LOCUS       DAOPNX010000001        73660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_442_length_73660_cov_7.352245, whole genome shotgun sequence.
ACCESSION   DAOPNX010000001 DAOPNX010000000
VERSION     DAOPNX010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 73660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 73660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73660
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_442_length_73660_cov_7.352245"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..559)
                     /locus_tag="PKN53_00005"
     CDS             complement(71..559)
                     /locus_tag="PKN53_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOD87371.1"
                     /translation="MKLLTLIRHAKSEQDTDDSDFDRVLTEKGVEDAYKMGEHIAKHL
                     PKPDLIISSPAERAAHTAEIIAEQIGYDEDEIEFVDEIYLCSTTEFLEVLMEQNVKTK
                     HIFLVAHNPGLTNLANLLTNSNIDNMPTCSVAHVELDFHKWDDIEAETGRVLEFFTLR
                     TI"
     gene            complement(703..2739)
                     /gene="metG"
                     /locus_tag="PKN53_00010"
     CDS             complement(703..2739)
                     /gene="metG"
                     /locus_tag="PKN53_00010"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOD87372.1"
                     /translation="MMKEFKRYTVTTALPYANGPVHIGHLAGVYVPADIYVRYLRLRH
                     KDVIFIGGSDEHGVPITLKARNEGVTPQQVVDRYHNIIKESFKKFGISYDIYSRTSSQ
                     IHHETAAEFFKTLYNKGEFIEKISKQYFDEAENQFLADRYIIGTCPKCEYEAAYGDQC
                     ENCGSSLSPEELIDPRSVISGNKPILKETKHWYLPLDKYENWLKQWILEEHKEWKPNV
                     YGQCKSWLDAGLFPRAVTRDLDWGVKVPLHEAENKVLYVWFDAPIGYISATKELRPDW
                     EKYWKDAETKLVHFIGKDNIVFHCIIFPSMLKQEGSYILPENVPANEFLNLEGDKIST
                     SRNWAVWLHEYLEEFPNKQDVLRYVLCANAPETKDSDFTWKDFQARNNNELVAILGNF
                     INRALVLTHKYYDGKIPVPSEITDKEKEIREEILKIKSDLEKNIENYKFREALKDFMQ
                     ISRIGNKYLADTEPWKLIKTDSERVKTIMYYSLQLCAISVVLGEIFLPFSMEKLRNML
                     NMEKTDWEDVLKFDLLKPEHKINQAELLFEIIDDAKIEEQLNKLAKTKEMNESSAKTV
                     SPQKEEITYDDFVKQDIRVATILEAEKVPKADKLLKLLVDTGIDKRTIVSGIAEYYKP
                     EEIIGKQVCVLVNLAPRKLRGIESAGMILMATDKDGSLKFVSPENLTANGSVIS"
     gene            complement(2795..3766)
                     /locus_tag="PKN53_00015"
     CDS             complement(2795..3766)
                     /locus_tag="PKN53_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87373.1"
                     /translation="MSELFKKFTIKPPKSAQYKNQYYADYIELVSLVSPDFVSQSDIK
                     DRLFDEGVKAEVEHPEDGEYGLLDSQINDKAEGYVNTYFEYLEKRKLIYGDNYPFIFD
                     KNKGIKVIDKSELSEQQKLYIYLLIASSLSSFRDIKQFITDDFEKLSELVLKAYLPSN
                     AKVFGFGSNSKYKGKTKEKIIKLGKDINVYARDDRIIDLIPDESTEDKGLDVVGWIPF
                     EDNNPNTIIIFGQCACGKNWFGKQIDTRRYDKFYTHYLTPFIHAMFYPEDFDNYGGKF
                     NLDLDLIENIVFERRRLLNLADENVFKHLSYSKHIVEECLNYREDIV"
     gene            complement(3774..4850)
                     /locus_tag="PKN53_00020"
     CDS             complement(3774..4850)
                     /locus_tag="PKN53_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HOD87374.1"
                     /translation="MSNVKHEKIPVTNLILDSKNPRLPKSMSNKSEKEIINFLLSDAS
                     LIELMLAISKNDFFEGEQLLVVPDEGDKYLVVEGNRRLSAVKLLHNPELGEVYKSKIE
                     QVIREAEHKPTDLPCLIFEDKDEILKYLGYRHITGIKSWKLLEKARYLAKLKNDYFPS
                     YSISSASREIAKMIGSRKDYVVRVLAGYKLYEIIENNGFYRISGLDDTTFYFNYLADS
                     LSRSNIAEFLGVDFNKDNPTENVQYTNLKEWTNWLFNKELPNKIIGDSENLNTLNKVI
                     ASPEALKAFRDGENLFVAIEFTDEFDVQFENAIKNSIRHMEKADLLTVKVVHFYSSLD
                     EDLNEIYQRIKKIKSAKGLRDDEF"
     gene            complement(4857..6440)
                     /locus_tag="PKN53_00025"
     CDS             complement(4857..6440)
                     /locus_tag="PKN53_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DndE family protein"
                     /protein_id="HOD87375.1"
                     /translation="MQINIKTSEKSQDIIRRLTPKLGDRTPENVIARIAFAYSLQKGV
                     KFNINHLFSQTDSKGKEYKDHILFDSRHRDFYIALICQHYEIYKTDENIPKYIKLHID
                     NGLELLDKIFDTSEYTFFDFLNEHIDKGIRHLVDVTVSAEHVRNAHQNIEKSYFSNII
                     KLEVGKTIDTKEPVYLSVNDNTKYNNCHIAVAGNSGTGKTQFALEFISQIYDKSNGHV
                     NYIYLDFKGLKQEDIKTYEKFFKDTKTEFVDVPRTQFPVNPLTFIDNVNESDKNMGID
                     KFVDIVCKYSNLGIKQRGKLREATSEAFVEQRDGTYPTISQVNEKLKETYDKQDTLTE
                     MMNDLSRYKIFLEDRKNESNFLNKNLYLSLSGDLPTSVRFTSLFLIINYIYNTFMNMD
                     NSPSENNIKSLRYVILIDEAHVLFKEKKYEEILEKMLREIRSKGVSIVLLSQGIEEYN
                     QKGFDFSSMCEIAFLLDIKDKNNTKAMEKFLGLSGKDTNKIVQSMSKIEKGQAISNIK
                     EFDKAKLFEVNQYWKRNKI"
     gene            complement(6430..8541)
                     /gene="dndD"
                     /locus_tag="PKN53_00030"
     CDS             complement(6430..8541)
                     /gene="dndD"
                     /locus_tag="PKN53_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA sulfur modification protein DndD"
                     /protein_id="HOD87376.1"
                     /translation="MFIKEVELNNFRIYKGVNRITLSPQCEKNIVIVSGQNGFGKTTF
                     LMSLVWCLYGKNMEQVDAMYKKEIEDKGNYTRYIMDSMNRKAAEDGETEFSVMITFSE
                     VNVQNVLCDVIVKRSYNIVTGTSDIVEVFISGQPQELIENLSQDNQKGEEIFIREFIL
                     PIAIAKFFFFDAEKITSLAEINQKDQRRELSRAYSEVLGIQKYETLKDNLEDKLDEYR
                     KKSAKNEDKQKLNQLEADIKNVELEIENCEIKINDLTEEIRSQKYESDDISLKLVKAG
                     DTMSLEELLNLRDEKNKLEEQKSKLQEQLKETFDFIPFALAGETLANMVEQIENERLL
                     SEQKYKQDDVKEKFTKIRQEIESEKQKLNFVPDIKTADFYIKQFEKLVKKYFYSDIQE
                     IDENMKILHDFSDSQTNEVNQLIVGIKNNFQVKFKSLNNEYSYVKNQIDSINRKIRDA
                     EKNAQDLHISDLRDKKEVLDGQISEKEREIGSLNQKIDDWREALKKHRQDQTAIRKQI
                     DDSAQYSEKEQKTKQLIKTLEEFILKFKQQKKESLQTKMLNSLQRLLHKKDFVQQVDV
                     DILTSEDIDIVLYGENNREIDKSGLSMGERQLYASALLSSLVSESEIDFPVFIDSPMQ
                     KFDNQHAENIIKHFYPNVAKQVVIFPLLHKELTENEYNLLQSNVCRTFLINNTSSDSS
                     EFLETEPNNFFSKYNQISNAN"
     gene            complement(8541..9905)
                     /gene="dndC"
                     /locus_tag="PKN53_00035"
     CDS             complement(8541..9905)
                     /gene="dndC"
                     /locus_tag="PKN53_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation system sulfurtransferase
                     DndC"
                     /protein_id="HOD87377.1"
                     /translation="MSNRINYIISELKDQYLIEDNLRSWIIGFSGGKDSTALLQLVWL
                     AMEQIPKELRKREIHVICNDTLVENPIIKEYVDDVLKKIEQTAVQRDMPISVKKTVPR
                     LEDTFWVNVVGRGYPVPNNTFRWCTDKMKIKPTSRYIVEEVDKNGEAIILVGTRRSES
                     VNRANTIKKHEVKGKRLTKHPNQVNTFVYSPIKELELEEVWYIINAISSPWGSNNSEL
                     FKIYADASADDYECPTMVSDDKHRSCGQSRFGCWTCTVVKEDKSLTAQVENGKEWLKP
                     LLDLRSSMFDQRNISENRDTTRRNGQKAVTADGHNQGNYTIEYRSKLLKQVLEVQKYI
                     QQFDPSIELIKNSELIAIQVIWRRDLAMPNKNYRELTSYKTVSEIYNEVYNKELDMKV
                     LDEKIQKEIDLLKSVCNDESHDFELIHELLELQKQKALLNRKRGLKDEMEQVIEKYLK
                     KETV"
     gene            complement(10081..10506)
                     /locus_tag="PKN53_00040"
     CDS             complement(10081..10506)
                     /locus_tag="PKN53_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87378.1"
                     /translation="MRTKALLISLIAIFAISFSSCNYTKKTMKTPNNYVEFKKDDFTF
                     SEQVVGRGAQTKVFGIDWSHLFVSKTGSIQALPFSFPIIGNLPIAGSEAYAIWDLMEK
                     NGAGYDVVFYPQYETQTKGVPFIFQKSTTTVKARLAKIK"
     gene            complement(10672..10744)
                     /locus_tag="PKN53_00045"
     tRNA            complement(10672..10744)
                     /locus_tag="PKN53_00045"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10709..10711),aa:Lys,seq:ttt)
     gene            11005..14889
                     /locus_tag="PKN53_00050"
     CDS             11005..14889
                     /locus_tag="PKN53_00050"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOD87379.1"
                     /translation="MKKIHLLLASLGLFLLVSNNAFSQPSYGGQPLSFTKNNPLSNEI
                     DHITINPPEMQATIAEADYLEQNDGLYRIATIIPVNLNMENSGTWDYLEDGTKIWRLH
                     INMEGAKGLALYYKDFHLPKYSQLFLYNQNKKQVIGAFDDRNNPKMFSDFSTEIIQGA
                     DTYIEYIQYPQATEDANIDIYNISYVFRGVSQLIGKYENSTKDPGNLGASGSCQVNVN
                     CPEGADWQTQKRGVAEIYCVQGFFGGLCTGSLVNNTANDGTPYFLTADHCGGSATNFS
                     NWQFYFNYEASGCTNPGTAPAYNTVTGAVKRARGDENTGSDMLLLELNTDQATLAGYN
                     AYYNGWYRGTDASPSGVCIHHPSGDIKKISTYTAPLTSATWQASSPANAWWRANWAQT
                     QTNWGVTEGGSSGSPIFNNNKLIVGTLTGGASGCGNAAAQQYDLYGKFDYHWQSNGGA
                     PADQLKPWLDPNNTGAETCPGFDPNNTGVVAPVADFVGNPTTVVVGGTVNFTDQSTNN
                     PTSWEWTFDGGTPPSLTGPTAQNPTITYNTAGTFTVTMTATNAAGSDTKTRTDYITVV
                     DPGTLSANFVADQTTINVGGTVNFTDQSVGNITSWSWQFPGGNPATSSVQNPTVTYGA
                     AGTFNVTLIVSDGTDQDEEVKVGYIHVTDNAAGLHASFVASSYNIIAGECINFNDQST
                     GSPNSWSWSFPGATPINSTNQHPTNICYPNAGIYDVVLQVQNADGQDTYICQECITVN
                     PDPNSPIAEFEADKRVIPVGGVVRFTNLSENGPFNQWAWEFEGGTPAVYNDSTPPPIA
                     YLQVGTYNVTLRCRKTNNVQDINTKVDYITVIPKATTPPTANFTANYTVVQPNDPINF
                     IDLSSGNPYQWTWEFPGSDQGTSNQKNPMGITYPEEGTYTVKLTVGNNFGTDIMIKEM
                     YITVSVDDPCTDEPTVAFNASARLIPAGGSVQFFDESEGFPTYHTWSFIGGSPSSSNE
                     GSPSSPIRYSTPGIYDVSLTVSNSCGSSTVVKHNYIYVFSSPVGQYCDTITTIKPGEV
                     VEPRVPDGQWGYLAGHNGLKIREYANRYDAHTFSEVSAVLVPLKQVKYDATDSYVKFF
                     VADANYSTPDSILGEKKVFLHQLTKNETNLIKFEPPISVNGPFYVGYKINYPDEDLDG
                     FSDDMLVVGIPMRPNDPAQNQLYMKKGSTWYTPNDFKSINPASSNFSSALPLKPIACL
                     VDIEEIIAENDINIYPNPTNGVVTIKIKDTYYSSFSFEIYDIMGRKVVSEIYKNGFDE
                     FTTDLSNYPEGLYIIRMKLGNLNLNKKILLTK"
     gene            14991..16121
                     /locus_tag="PKN53_00055"
     CDS             14991..16121
                     /locus_tag="PKN53_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOD87380.1"
                     /translation="MKFIYFLIFNFLVLFLLNSCNNQHIKTEEGQTIPNKYATDFEIQ
                     EISDGYILKVKNRFDQTNSGTYNYYLSYEPESSPNSNVTIPIPVSKVACLSTSHIAFI
                     STLNKTSSIKGISTPELVCNSDVSKLYAQGKIYNIGYDNQINYEKIISISPDVVFAFG
                     VDNTSVSKFQKLTDLGIPVVFVNDFTEAKPLGRSEWIKFFACFYDKLNFATEYFDSIE
                     NNYLNIKSSIPQTTEKPKVLVSLPWKGTWWVPGGNSFFANFINDAGGEYIFSNNNHSD
                     SQGLNIEEVFAKAKNISFWLNPNNVNTKTEILSIDNRLEFFEPYKTAKIYNNNLKENQ
                     AGGNDFWESGILHPDIILLDLKTIFYQDSTNIHNLYYYKQIN"
     gene            16132..17154
                     /locus_tag="PKN53_00060"
     CDS             16132..17154
                     /locus_tag="PKN53_00060"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOD87381.1"
                     /translation="MKKTKLTIKFILLSVFLIFLGLLDLLSGSIQISVSDIFNYIFSS
                     NTESEFYLLLKEFRFPRMSMAILAGASLSISGLIMQTLFRNPLAGPDILGISSGASLG
                     VAIVVLGGSYLFGNHFYHASNYITLIAAGIGAASILGLILIISYKLRDILSILIIGIL
                     LAGIISAIVNILQYFATGINVKSFVVWTMGSLTASSSKDLLLASPIILIFVLLAFSLS
                     KNLNLMLLGENFAKTMGVNIKLLRILSFTIVSILTGVITVYCGPIGFVGIAVPHISRW
                     IFNTSNHFVLIPASALLGGIFMLCGDILSNLFTSNGNLPINAITAILAAPFIIWVVFK
                     NKRTFV"
     gene            17154..17861
                     /locus_tag="PKN53_00065"
     CDS             17154..17861
                     /locus_tag="PKN53_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD87382.1"
                     /translation="MENSILNIINLKFGYKIPLCEQEVNLSANIGDLICLVGLNGSGK
                     STFLNTISGILKPISGKIIIDGQDVKKLSLNNKSKLISFVPTKIDFTANLTVRELAEM
                     GRSPYTNVFDKKSEADIKLVEKNLNDFDLLKLQNRQLWSLSDGERQKAMIARALIQET
                     PLMILDEPSAFLDYEMKHQLMRKLKEISTNKCLIFSSHDIETSTKFATKIWFINKSKF
                     EVLSPMEFIENHYKKSI"
     gene            17939..20311
                     /locus_tag="PKN53_00070"
     CDS             17939..20311
                     /locus_tag="PKN53_00070"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOD87383.1"
                     /translation="MKHIKLIFIIFIASCNLGAFAQKYTISGYVTDKASGEHIFSANV
                     YDMRTKQGTTTNEYGYFSLTLDADSVNLSISFVGYAAFTSDIYLTSDLNLNIQLDPNI
                     MLAEVVILDKKSDEIVKSTQMSMIEMPIHQIKALPVLLGEADVLKSLQLMPGVQSGSE
                     GSSGLYVRGGGPDQNLILLDGVPVYNASHLFGFFSVFNTDAISNVKLIKGGFPARYGG
                     RLSSVLDIRMKEGNLKEYHGEGSIGNVSSKFTFEGPIVKDKASFVVSARRTYIDILAA
                     PLVLAVNRAAGNSDKFRAGYYFYDANAKLNYKISDKDRLFFSIYTGKDKAYTRSKQSY
                     IYNDTTKTEAINFNLNWGNITSALRWNHVFHKKLFGNLTAVYSKYKFETIQKYEFLSG
                     KTIEEEYYFKYLSGIDNVGANLDFDYRPLPKHSVKFGANYLYHTFNPGGSSISMKFGG
                     NSITQDFSNKKIYANEFYVYAEDSWDIAALLKLNFGVHYSGFAVQKRFYQSIQPRASA
                     RFLINDKMSLKAAYSHMSQYIHLLTNTSIGLPTDLWLPSTDSVRPELSKQAALGFVYN
                     IDNNWDLSVEGYYKTMSNLIEYKEGAGFFQLAQNWESKLEIGEGYAYGAEVLLRKNYG
                     KLSGWLGYTLSWSWRKFKNINFGEPFPYKYDRRHDISLVLMYEFNEKVDMGLTWVYGS
                     GNAVTLGVARYNSLNLDYYNPGEYYYGEIEYFKGRNGYRMPAYHRLDLSINLKKKTKW
                     GSRTWSFGLYNAYSRQNPFYLYFGYDNNKRVLKQVSLFPIIPSVRYAFKF"
     gene            20324..21319
                     /locus_tag="PKN53_00075"
     CDS             20324..21319
                     /locus_tag="PKN53_00075"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HOD87384.1"
                     /translation="MKASIFKYILLFSILASLIFSIACEKTIDIDIEDSKPKIVLNAE
                     INPDSTIKVHISRSRHILDIANLSTLNDAEVKLFEDETFIGNLIYDASISCYCINYKP
                     IQGKNYKFVVSHQQFETINATSQILEKIKPISIDTSRSFGQYNEEQINISIKFKDPAN
                     EKNYYMLKLRSKYKAEIWDPDLITIDTIYEGPDTTIIDISQGGYYYADAIDPMHFSSN
                     DLNLEDGDYLRSLVIADEFFDGKEYVLKISLDAFSLPYTSDTTVVYIDFYAISQDYYK
                     YLRSEKKYQNSSGDPFSEPVMVFTNVENGLGILGSAHAHTDSIIITGRYGGHYDE"
     gene            21309..22142
                     /locus_tag="PKN53_00080"
     CDS             21309..22142
                     /locus_tag="PKN53_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOD87385.1"
                     /translation="MMNNETKLDITLTVLEVKDESLKPYIYNYWLFISSEKPNLCAAI
                     TAPPLGFPMLQFVFGNCESYYQIRNKNTTSIITGIYSKHATLTPAPKMGLVTINFKPY
                     GFYNFFGKTPPNGTQDSIDGYDIFKKENLNSAIENISITETNDELVNCLEDFLCKNQK
                     VNVKKNDYFDSLVDKIFAKKGLIDIDKLINEKFSIRTFQRYFSKVLGVSPKTFSRILR
                     HKYIIQLMYTKPNISWNDLCFQGFYFDQSHFIKDFKDFTKCKPTEYDYLKTSIIYELF
                     K"
     gene            22380..23843
                     /locus_tag="PKN53_00085"
     CDS             22380..23843
                     /locus_tag="PKN53_00085"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOD87386.1"
                     /translation="MLNQTDINYLKSFGIIENSIEEQIKLLKKHESFLNINNFASTKN
                     GITRIEDPQFYVNVFNNNSKNYKLAKFVPASGAATRMFKRYITYKLDPNLKNLNDGGF
                     YSVKNGLENIKNFAFYKKLKPLCTEPENLETVVEKILYSGLNYANLPKGLIDFHNYPD
                     SPRTAFEEHLHEANLLVQDQENFNIHLTVSPEFLENFQSKLKEINQKTGYKFNLSFSE
                     QEQSTNTISTYLNGEIVRDDNGNIMLRPGGHGSLLTNLNSLDSDIIFIKNIDNLTHGD
                     YLEETILWKKVLAGVLIEIVEKIQEIYSNLKNNPTTENLNKAKDFLEKHTNAIFDNSE
                     DLKTEVLNYINRPIRVCGMVKNTGEPGGGPFWVKDKNGKISLQIVEKAQINLDIPEQA
                     QYFNNSTHFNPVDLVCYVKDPEGQKFDLKNFVDKNSSFVSEKTHQGKTIKVLEHPGLW
                     NGSMADWISVFVEVPLITFNPVKELNDLLRKEHQPKE"
     gene            23847..24722
                     /locus_tag="PKN53_00090"
     CDS             23847..24722
                     /locus_tag="PKN53_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004382343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOD87387.1"
                     /translation="MTKAKISIRFFDDREVRAVWDEENNKWWFNVIDIIAILNEQGDY
                     TKANNYWRWLKRKLKSENNQVVSATHGFKFVAADGKKRLSDTLDSAGVIELAKSFPNT
                     NAIKFLDWFLYSDNTIDGQSKKKAYTLFESNLINEIEVGTTKGLQQIHAYLFGGLYDF
                     AGQIRQKNISKGGFKFASAQFLENTLQQIEKMPQNTFDEIVEKYVEMNIAHPFMEGNG
                     RSTRIWLDMMLKQKLKLCVDWSKVNKNDYMKAMEKSVTNSETLKSLLKNVLTHEINSR
                     EMFMKGIDYSYYYED"
     gene            complement(24757..25854)
                     /locus_tag="PKN53_00095"
     CDS             complement(24757..25854)
                     /locus_tag="PKN53_00095"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOD87388.1"
                     /translation="MNFRLNILLFLLIFSCSLKAQIAPKYSNEFMGIGVGARALGMGN
                     SVIASSDDINATYWNPAGLYKLDKKIEIGAMHSEYFAGIAKYDFLGASYKIDDKSALA
                     LSMIRFGVDDIPNTLDLIDSDGNIRYDRISSFSVADYAFLFSYSRKLNIEGLSVGGNA
                     KIIRRNVGEFANAWGFGLDASAMYHYKNWKFGANFRDITTTFNAWKFNQELLRDVFEA
                     TGNEIPENGLEITLPRLLLGAGYLYNISDDFSVYGELGMLTTTDGKRNTLIKTNLISI
                     DPSFGLEVSFRNIVFARAGVNNFQQIPEFDNTKSTSWQPNIGLGVNLFGLKIDYAFTD
                     IGNQSIALYSHVFSLSYRFNAEKLSQSKSRL"
     gene            25968..26672
                     /gene="pssA"
                     /locus_tag="PKN53_00100"
     CDS             25968..26672
                     /gene="pssA"
                     /locus_tag="PKN53_00100"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOD87389.1"
                     /translation="MKIYKSIPSLITSGNLICGAIAIFLAFSNNLEFAVYFVLLASVF
                     DFFDGFAARKLNAVSEFGKQLDSLADLISFGFAPSAMIYVFIENNEILPKNFSYLSIL
                     LVLFAAYRLAKFNTDESQTTEFKGLATPGMALFIMGIVYPKSLGLISTEQIFLNKIFI
                     IACLIILPVLMVSNIRFFSFKIKKFSFKNIVWQLVLLIFGIILLIIFGINGLAYLIIL
                     YLALSILKNLTTKKTI"
     gene            26674..26925
                     /gene="purS"
                     /locus_tag="PKN53_00105"
     CDS             26674..26925
                     /gene="purS"
                     /locus_tag="PKN53_00105"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOD87390.1"
                     /translation="MKFLAQINIMPQKALLDPQGKAVLNSAKKIGFDTVNNIRVGKHI
                     EIEVQAKSEQNAAEIIDDLCKKILINPIVESYEFKLFKI"
     gene            26978..27051
                     /locus_tag="PKN53_00110"
     tRNA            26978..27051
                     /locus_tag="PKN53_00110"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27012..27014,aa:His,seq:gtg)
     gene            27138..27213
                     /locus_tag="PKN53_00115"
     tRNA            27138..27213
                     /locus_tag="PKN53_00115"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27173..27175,aa:His,seq:gtg)
     gene            27310..27984
                     /gene="clpP"
                     /locus_tag="PKN53_00120"
     CDS             27310..27984
                     /gene="clpP"
                     /locus_tag="PKN53_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOD87391.1"
                     /translation="MTKDFRKYAIHNRGINGLVFDEYTSIYNNYISPTIIEERTLNIA
                     TMDVFSRLMMDRIIWLGAPIDDYVANIIQAQLLFLESTEPNNDIQIYFNTPGGSVYAG
                     LGIYDTMQYISSDIATICTGMAASMGAILLCAGATGKRSALKHSRVMIHQPMGGASGQ
                     VSDIEITTREILKLKKELYEIIAYHSGKDYKTVEKDADRDYWMTAEEAKSYGMIDEIL
                     IKNVSL"
     gene            28021..29235
                     /gene="clpX"
                     /locus_tag="PKN53_00125"
     CDS             28021..29235
                     /gene="clpX"
                     /locus_tag="PKN53_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOD87392.1"
                     /translation="MEKCSFCGAPKHKNDLFIAGMYANICEFCVERAYEIVQEDRLAN
                     KKSEFSLKSLKKPKEIKKYLDQYVIGQDNAKKVLSVAVYNHYKRIAQIIKEDDVQIEK
                     SNILLVGETGTGKTLLARTIAKMLEVPFAIVDATVLTEAGYVGEDVESLLVRLLQDAE
                     YDVKAAERGIVFIDEIDKIARKSDNPSITRDVSGEGVQQGLLKLLEGSVVNVPPQGGR
                     KHPEQKMIAVDTKNILFICGGAFDGINRHIAKRINTQVIGYGKEKREEKIDKENFVQY
                     IIPDDIRKYGLIPEIVGRLPVLAHLNPLDKDALRRILTEPKNSIIKQYIKLFEMDGIK
                     LTFKEEALDYIVEQAVKFKLGARGLRSICESIMLEAMFEFPSKKVNEFVLDKKYAESK
                     LDQTAITKFKSA"
     gene            complement(29232..29912)
                     /gene="ubiE"
                     /locus_tag="PKN53_00130"
     CDS             complement(29232..29912)
                     /gene="ubiE"
                     /locus_tag="PKN53_00130"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOD87393.1"
                     /translation="MQKENTPQMFDAIAQTYDKLNHRLSLNLDKVWRRKFVKFVSDRN
                     YDVVLDVASGTGDLLLELQKLKSQKYIALDPSKNMLEIAKAKVPTAEILISKAEKINL
                     QDDSVQLITVAFGVRNFASVDEAFKEFYRISQKNACLAIMEFALPKNFLCRFGFKLYL
                     NLIIPFYGKIISKNKQAYKYLSKSIVDFANNVDLTNKLEQAGFSDIKVKNLLCGAVKI
                     YKAKKIIF"
     gene            complement(29976..31910)
                     /gene="yidC"
                     /locus_tag="PKN53_00135"
     CDS             complement(29976..31910)
                     /gene="yidC"
                     /locus_tag="PKN53_00135"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOD87394.1"
                     /translation="MDRNTVIGLLVIGALLVGYMFLTKPTPEQIEARQKMQDSIAQAK
                     IKEDSLARVQIAMQDSIDKAIEQEKIENHENLSQEERDSINALKLEDKYGIFANAFDS
                     NDDGFTIIENNKMIIKVSNLGGKIASVELKDYKTYYQDPLILYKNDSLSTFGLELFVA
                     GKSIITNDMYFAPSTKEDTIKVVDKEEKLSMRLKAADDKYIEFLYTVYPDNYMLDFDI
                     NFVGLQNEINTLVNSYANMRWEVKVPRLERGIDWENNNTTIYLKIDKDIESLNAAKAK
                     DEFKTSGSAHWIAYKQQFFSSVLIADEALSSPVVNAEKIDDPSGNYLKTFSSTFSIPL
                     ENAEKQKVGFRMYFGPNKFSKLKKYDIKLEKLVPLGWGIFGWVNKWIIIPIFNWLGGF
                     INNYGLIIFLLTLIVKIGLAPLTYKSYLSSAKMRVLKPQVDALNEKYPKGKEMEKQQA
                     VMALYKKAGVSPMGGCLPMLLQFPILIAMFRFFPASIELRQQSFLWANDLSSYDAIVE
                     WSTHIPIISTFYGNHISLFTLLMAASMLVSTWITSRNQPQNNSMPGMKFIMYFMPVMM
                     IIWFNKYSSGLSYYYFLANVLTILQTLIIQKFIINEEKLLAQMETNKKKPAKPKSKWQ
                     QRIEEAQRAQQQKLQQQRKK"
     gene            complement(31922..33541)
                     /locus_tag="PKN53_00140"
     CDS             complement(31922..33541)
                     /locus_tag="PKN53_00140"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOD87395.1"
                     /translation="MANTKFIFVTGGVTSSLGKGIISASLAKLLQARGFTVTIQKLDP
                     YINVDPGTLNPFEHGECYVTEDGAETDLDLGHYERFLNVYTSQANSVTTGRIYQEVIN
                     KERRGEYLGKTVQIIPHITDEIKRRVKLLSTKGKYDFVITEIGGTVGDIESLPYIEAV
                     RQFRWEFKNRCMVIHLTLVPYLAASGELKTKPTQHSVKTLLEAGVQPDMLVLRTEQPL
                     TNDVKVKVANFCNVQKEAVIESRDMSTIYEVPLEMQKEKLDEFVLSHFGIAIGEKPKL
                     TAWKEFLKKTKNPKQSINLAFVGKYVDLKDAYKSINESFIHAGAFLETKVNINYIQAE
                     DVNDNSSTEFFKDAHAIFVGPGFGHRGFEGKISAVKYARENNIPFFGICLGMQAAVVE
                     FAQNVLKLEDANSIEMDRTCKNPVISLMEEQKMVNDKGGTMRLGSYSCKLVKGSIAHE
                     AYKSLKISERHRHRYEFNNKYKDAFVKAGLVATGINEEIDLVEIIEIPKHPWFVGVQF
                     HPEYKSTVLNPHPLFIDFINAAIDFKRQNKS"
     gene            complement(33551..35854)
                     /locus_tag="PKN53_00145"
     CDS             complement(33551..35854)
                     /locus_tag="PKN53_00145"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOD87396.1"
                     /translation="MHKIIRNTFFIFSFLPVTLYAQQGTIKGFVYDKDDGEPCMFANV
                     SIKGTNLGAATDLNGYFSIPKIPIGDYKIIITYVGYDSIVEDLRIESAKLYSKNFYMT
                     KSAFVLGETVVSAERQEMKTQTRISMVKVSPTQIKQLPSVGGEPDIAQYLQVVPGVTF
                     TGDQGGQLYIRGGSNIQNLVLLDGMTIYNPFHSIGLFSVFDSDIIRNADIYTGGFNAD
                     YGGRISSVMDLSIREGNSRRFTGKVSASSFGSKVLLEGPIVKYKENKVSASYLFSGKT
                     SYLEQSSKLLYTYINDGKGLPFNYTDLYGKVSLSSPEGSKINLFGFKFSDRVSYQGLS
                     QLGWDSYGIGANVSVVPLGSTVMIKSNFSYSDYKIGLQTLDNLPSKSGIRGYNFGLNF
                     LYFFGKNQFVWGVETLGFNTDFIYYNSIGRELKQEENTTELAAFAKYKLNLGSLLIEP
                     GLRLQYYASMSEASLEPRLGLKYNATERLRFKLAAGLYSQNLVAANSDRDVVNLFYGF
                     LSGDLNLPSSFKGEELRHKLQKSEHLIIGAEVDVTNHIDFNVEAYIKNFSQLSTINRN
                     KIYNDIPINGTKPDYLKKDFLIETGYAYGLDFLLKYDKNNLYVWFVYSLGWVKRDDGV
                     QLYSPHYDRRHNINLVTTYKLGKKQSWSISARWNLGSGFPFTKTAGFYEEVDFYHSLN
                     YPYWQGNGSLGIIYDELNTGRLPYYHRLDLTVNKIITFNKYTKLELNAGVTNVYNRRN
                     IFYFDRIDYNRVDQLPIMPSLGLSFTF"
     gene            complement(35847..37847)
                     /locus_tag="PKN53_00150"
     CDS             complement(35847..37847)
                     /locus_tag="PKN53_00150"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOD87397.1"
                     /translation="MIENAKNGLENQENTTKNTDQDNNNVEKVEINAENGSVETEQNK
                     TIEKVEIKSTEDKKINTDEVFISEIDADEDDEDVDNDNVPAENDFSNLNNEEIIEKLK
                     QLIYESNIEETRKDIDLLKRLFYRNLRRQQDALRQEIIEATGNELEVSLPKDPNEDYL
                     KELWAEYGKKRAELAKQQEAERLENLKKKEEIIEKIKVLANGEESMNKTFNEFKELQQ
                     EWLKIGPVPAEVSNELWNNYQLQIERFYDLVKLNKEARDFDFKRNLEQKIELCEKAEE
                     LLLSTDVVASYRKLQDYHELWKELGPVPNDKRVELWDRFSDISKKIRKNYQDHFEKLK
                     EEREANYKQKLILCEKVESILVENTATNGKEWAELSDKVLELQKIWKTIGMVPTAVNN
                     EVFERFRAGCNKFFEAKNEFFSEINAELKDNLQKKTDICLSAESLQDSTDWKKTTRMF
                     LDLQKQWKEIGPVPRKNSEQIWKRFRAACDHFFNAKSEFFKNIETEHKENLAKKEALI
                     EEIKNYKPLDDQSENVNKIKEFQNTWTEIGFVEISQRDRLYDEYRGAINELYKKLKLN
                     KSALEISNFINKVESIKGSGALNELAREKNRILQKIKELNAEILQIENNMGFFASGTE
                     SLVKDFNKKIEKAQEEIKTLKEKKKAIDLAERELKNKDGNDA"
     gene            complement(38219..40378)
                     /locus_tag="PKN53_00155"
     CDS             complement(38219..40378)
                     /locus_tag="PKN53_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOD87398.1"
                     /translation="MQEFFSELLAEFTLPLSNPILVFSVILFIILLSPILLQRFKIPG
                     IIGLIISGIIIGPYGFNIIANNSAIELFSTIGLLYIMFVAGLELNLAQFKMNKYKSLT
                     FGVFTFIFPFAIGYALCHYVLGYGINASVLTASMFATHTLVAYPIVSKLGVTKNEAVA
                     VTVGGTIFTDTAVLLILAIIIGNTQGSLDQEFFIRLGICFVLFLIIMFFLVPKVARWF
                     FSKLESEKHAHFIFALSVLFFAAFLAEISGLEPIIGAFVAGLALNPIIPKTSALMNRI
                     EFSGNALFIPFFLISVGMIVDVSVVVQGWGAAIVAGVLIVGAVFGKWIAAFLTQVIFR
                     YSGTQRQLIFGLSTSHAAATLAVILVGYRIGILDDNILNGTIILILVSCIIASFVTER
                     AAKTLALTEEDSQIGEKAYNKFAEHILIPVANLASVPKLLEFAVLIKNLKSSRPISLL
                     SIIQNDSNAEQNLLKTKEKLEDFIKYGAAAEVEVNAITSIDYNVVSGISRVSKEMMAD
                     ILILGWPGKAGIINKLAGERVNKIIYSGNKNVFVTSLENAFVANKRIILVTPHLANKE
                     KGFKLWTEKVLKLASELSAQILHFGTEEVDKSLKTLVKKNKFNVKIEFSEFSEWEDFL
                     ILARYIEKDDLMIVASARRNSFSYVPYFENIFSKIERYYSEYNRIVIFPQQIEGSIAQ
                     YDDLANNPLTKGIEAIENIGKGIGKIFVKSEEEEQNS"
     gene            complement(40684..40962)
                     /locus_tag="PKN53_00160"
     CDS             complement(40684..40962)
                     /locus_tag="PKN53_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HOD87399.1"
                     /translation="METSKFDIADYLDSNEMIAEYLNTVLAEGDDSDVIVAIGHVAKS
                     MGMTKIAKETGLSRSSLYKALSEGSNPRFETIMKILRAIGGQIQINPV"
     gene            complement(40968..41267)
                     /locus_tag="PKN53_00165"
     CDS             complement(40968..41267)
                     /locus_tag="PKN53_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOD87400.1"
                     /translation="MYIIYKTSDFDKWLRKLRDLKAKAIILFRIQKIEEDEHFGDCKH
                     VGDGIRELKIDYAKGYRIYFKESDGKIIILLIGGEKSTQQKDINKAKNILKKLKE"
     gene            complement(41608..42582)
                     /locus_tag="PKN53_00170"
     CDS             complement(41608..42582)
                     /locus_tag="PKN53_00170"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD87401.1"
                     /translation="MNKLKLASIVLIKHSKFFRKIFEIFFSRITFVKEKLLEINFLEQ
                     YIHSLYSEESLKEKRFYNIGAGSQRSKYDIWTYIDLESSKYSKEGIDIFYDLESLAPI
                     PLPDNHAEVVFNSFVIEHISIDATKNLCKEAYRILKKGGVFHSKVHCYDYSYKLYKNH
                     LITPKAPFECRESKEKLNNFIKNHKGRVKATFNDKNEYVIKSLVNSEELVFNSENTFL
                     YHNAVAAMDNLYAKSPDLRASLNDCDTGNVETFYKNVLNFVDENKKESYQHNADYFSK
                     EELFAYIKNLGFSEVYFTQAFQSISPALWEDKLNPIHKGFLFSIEAIK"
     gene            42704..43708
                     /locus_tag="PKN53_00175"
     CDS             42704..43708
                     /locus_tag="PKN53_00175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD87402.1"
                     /translation="MLSQRFKNDGKPIYKLNEIQLQAKADVERKIENGQYEFEEINCA
                     ACKSNEYELLAEKDRYGLKYYTVICKNCGLVYVSPRMTEKAYAEFYNAEYRKLYVGEN
                     VASEKFFADQVFRGKRIFQFINTNYEIKNKKLDVLEVGCGAGGILSVFKENGHNITGI
                     DLGEEYLNFGKEKYNLNLFKSNLNDMDKNYKADIIIYSHVFEHILDLDKELDAIKSHL
                     KPEGILYIEVPGIKYVHNTYEMNFLKYLQNAHVYHFSLNSLTRILHNNNFELIYGDEQ
                     IRAIFKLNKNLQDKIFENEYSTILNYLHKTEKMRFLNVFRFKKLKWQIKKIFLKLSFK
                     "
     gene            43766..44869
                     /locus_tag="PKN53_00180"
     CDS             43766..44869
                     /locus_tag="PKN53_00180"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD87403.1"
                     /translation="MKALFTLALLFIACLSYSQQQLLDSVEYIYWGDYTDGTIRKAKL
                     DGSDIDTIVDDDSKIRRIRLDRRDRKIYWASGYYGAIRSANYDGSDISTVLQTSLDID
                     LIEVDQKNKKIYFTEDYTGTIKRCNTDGTELETIITGTGLVLGMAIDTVRDILFYGDY
                     TNKKIFRAKPDGNDITEIFSTDKQIFDLYIDIKQMYVYFNNRTTNAIHRIFYDGSGFT
                     DIIQCCSNNCVVGSLAVSVEYNFLFWIERENGILARSNANGEDRTDIIYVPGSNFSGM
                     DIGKEYLTHVKVDENKLKQIKIYPNPCSNQFFVDAEINSDVSIYNLYGQKIKTSKLTS
                     SKSIDIDELPTGIYFVEVKNENDVFKQKIQVAK"
     gene            44966..47647
                     /locus_tag="PKN53_00185"
     CDS             44966..47647
                     /locus_tag="PKN53_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOD87404.1"
                     /translation="MRNLVFSFLILFMFSSCLKRVEVIEPSQFELNYNDKLAFAEAIG
                     SDFFEKLENYKSDSLVYQDLQYLFTYMPLSDFADYDFEFFLNQVKYAHLIKETFPWAK
                     DIPQDIFRHYVLPHRVNNENLDTARMVFYRELKDRLLPLNLTAEEAALEVNHWCHEKV
                     IYKPTDARTISPLGAVRSGFGRCGEESTFTVTALRAAGIPARQVYTPRWAHTDDNHAW
                     VEVWIDGEWKYLGACEPLPRLNMGWFTVSATRTMLVHTKQFGQDDEESSNYLGSNANY
                     TWVNAMKTYAPTKNLIILVVNKNNMPVWNAEVRFQLYNYAEMYPLHTAKTNINGMAKF
                     TTGYGSLEVSVSYKDKTISKTINPQDENIITLNIDTDPKFPAENVVYYPPVAGENHII
                     DPEEEKLNNERLKQEDKIRANNESSYYNQSKTKDFIKAFDYPESVAEILIGSKGNWME
                     IEAFLIEASYKNCKNEALALLTELSEKDWRDTKKEILSKHCELAIKHRNSNLADSIFN
                     KYVLNPRIHFELLTNYRPAILESLTDENIKNFKSNPEKIYQYISENIKTQFNINGKNI
                     DVQDFNNYNVSITPLGVNKVKHADPLSLKIYTVAFCRSLGIPARINLASNFAQYFHNN
                     EWVDFNFDKAKVEKAKLFFTNPDTTRDLKYRVHFAIARLESGFFNTVDLGWEIPISDF
                     SDGIEVLDGKYMLLTAYRNSDESVSVNRKYFDLKANETKTIEVTIPEDNSISLNIKAF
                     VHGNLTDNQTNSLIDSKLICKENTATAFCWINPTQEPSKHIIRDINLGLNNLKNNKIN
                     VVFITDGSEIQGLSKEIPTYTDKNFELLNKNSACSLSANSNVFPNIMLVSNNNCIFSS
                     KGYIINVSEILINEWKKSVKSDAMNGVEVLKVESNAN"
     gene            47637..48320
                     /gene="trmD"
                     /locus_tag="PKN53_00190"
     CDS             47637..48320
                     /gene="trmD"
                     /locus_tag="PKN53_00190"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOD87405.1"
                     /translation="MRIDIITVLPELIAGPLDLSIIKKAKDKGIVEINILDLREYGLG
                     KYKQVDDYPYGGEAGMVLMIEPIFNLIEKLKSERKYDLIFYTSPDGKPWTQKEANKIS
                     LLENIIILCGHYKGIDQRIREHLIDEEYSIGDFVLTGGELAAAMMCDSIVRLLPGAIG
                     DEQSALSDSFQDNLLAPPVYTRPEVFNNWKVPEVLLSGNFKKIEEWKEEQAYERTLKL
                     RPDLLKNDE"
     gene            48362..50245
                     /locus_tag="PKN53_00195"
     CDS             48362..50245
                     /locus_tag="PKN53_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOD87406.1"
                     /translation="MSSLLQGENLSKRYGEKLLFEGININISEGDKIALIAKNGTGKT
                     SLLNILTNTDTPDSGNITSKRDLRLAYLPQELNLDPEKTILEAVFFLDDEIISTIREY
                     HSVLSSNNHADLQAILDKMDSLNAWDYETKVKQVLSKLKIDDFSKKIKYLSGGQKKRV
                     ALAGTLISEPDLLILDEPTNHLDLDMIEWLEEYLTKTNSAIFMVTHDRYFLDRVCSTI
                     FELDMGQIFIYNGNYSYFVEKRAERIENLQANIDKAQNLMRKEQEWIRRMPKARTSKS
                     KYRIEAFDELKEQASQRIEDEKLQFGIANQRLGKKVINIYNISKSFGSEILISDFSYK
                     IAKNEKIGIIGNNGSGKTTFLNILTNKIKPDSGRVEIGETVKIAYYTQEGLSFDEDSK
                     PIDIIKNIAETVKLKNGKTLSASGFLQYFLFSPEMQYTYVKKLSGGEKRRLYLCSILM
                     TQPNVLLMDEPTNDLDIMTLQVLEEYLSEIEASVIIVSHDRFFMDKIVDHVFVFEGNG
                     KILDFPGNYTQYRYSPYYQRLNQEQKPEKKESPSKESLPKNVSQDKPKKVSFKDKYEY
                     EQLNKELEELNHKKQKLEIEINSGNFNHVELTEKSEEYSKLLEIIEEKEFRWLELDEL
                     INQ"
     gene            50324..51493
                     /locus_tag="PKN53_00200"
     CDS             50324..51493
                     /locus_tag="PKN53_00200"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF011105.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOD87407.1"
                     /translation="MLNIALFGPPGAGKGTQSKILIEEYNLSYISTGELLRKEIKNKT
                     KLGLEAQSIIAAGGLVSDEIIVQIIEKTITENPNANGFLFDGFPRTYIQAYILEGLMI
                     KLNTSLNCLISLAVPEEVSVQRLLERGRTSGRTDDNETVIRNRLNEYYEKTIPVINFY
                     KEKGIFSEVDGTQSVENVRKDIKAIVKKELSKRLLNIVLFGYPGSGRGSQGRAIAKKY
                     GLEYVATGPMLHEEIEKGSEIGKQIKDLFDNGKLIPDEIVVPLIEKKIENSKGVKGFI
                     FKGFPRTLVQSYILDGLLKKHSSSISKIIEIEVPTLELIKRLDERSKTENRMPYDSST
                     AKIVQRLQDHQTKTVPVIEKYNELHGVVKIDGMGSFDEVFKRISFEIENGFENLK"
     gene            complement(51572..52573)
                     /locus_tag="PKN53_00205"
     CDS             complement(51572..52573)
                     /locus_tag="PKN53_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOD87408.1"
                     /translation="MDITQYKDVYVFAEQRGGVVQKVAFELLGKARELADALNEKVVA
                     VLLGKDIENKAQDLIQAGADKVLVVDNDALKNYMTEPYTQALSQIINERKPDILLLGA
                     TTIGRDLGPRVSARVTTGLTADCTKLEIGENGEFLMTRPAFGGNLMATILCKDHRPQM
                     ATVRPGVMQRLEDTSARKGEVEKVEVSFNMEKINRVKLLESVVKPKTTKDITEAPVLV
                     SAGRGVHKEYYGHLRELADELNGEVSASRATVDAGNMDHDLQVGQTGKTVRPQLYVAC
                     GISGAIQHLAGMEESEYIIAINKDKFAPIFGVADIGIVGEVEKIVPLLTEKIRNLKK"
     gene            complement(52594..53376)
                     /locus_tag="PKN53_00210"
     CDS             complement(52594..53376)
                     /locus_tag="PKN53_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOD87409.1"
                     /translation="MNIIVCIKQVPDTTEVKINPQTGTLMRDGVPSIMNPDDKGGLEF
                     ALQLKDKYNAHVTVITMGLPQAEAILREALAMGVDRAILLTDRKLGGADTLATSNSLA
                     GALRTLDYDLIITGRQAIDGDTAQVGPQIAEHLDIPQVSYLEHLDFDGKKTLTIRKQT
                     EEGYQVLQMDTPCLVTVLASAVKPRYMHMRGIVAAYKKEIEVWGADKINVPEEKIGKA
                     GSPTSVAKAFPKSIKPAGSLFEGSAEEAVNLIVSKLKEQHII"
     gene            complement(53414..54559)
                     /locus_tag="PKN53_00215"
     CDS             complement(53414..54559)
                     /locus_tag="PKN53_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOD87410.1"
                     /translation="MDFSLSKTEELFLQMIREFAEKEIKPLAAEIDEQERFPIETVEK
                     MAKIGLMGIPVPVEYGGAGGTNMMYGIAVEEISRVCGTTGVVLSAHQSLCVAPIWENG
                     TEEQKKKYLPKLASGEWIGAFGLTEPNAGTDAAMQQTMAVLDGDEYVLNGTKIFITNA
                     EYAHVYIIFAMTDKSLGNKGISAFIVEKGTKGFEIGKKEKKLGIRGSATAELVFTDCR
                     IPKENLLGQVGRGFGIAMKTLDGGRIGIAAQALGIAQGAMDETIKYTKERKQFGRSIS
                     QFQNTQFQMADMETKVQAARLLVRNAHYKKDNGIPYSVDAAMAKLFASETAMEVTTKA
                     VQFHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVIAAKLFSKK"
     gene            complement(54754..55338)
                     /locus_tag="PKN53_00220"
     CDS             complement(54754..55338)
                     /locus_tag="PKN53_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87411.1"
                     /translation="MKKYLLITILFVFSLNISFSQKRMMYCYPVLNDYVEQNLFQEAL
                     DDIFAKDSLSNSDIYNLAICYSNLGDFEKAQKNLMDFIKKVEYNLTPAIFLDYRISAL
                     RESNYWENVYQLLIEKFLYESGPITDTALAIKIMEHGILDQKYRGLIITQPCELAPTK
                     QKRKVNERRKEFGFTTTVEEEIEKHNKNKVTFYK"
     gene            complement(55369..55992)
                     /locus_tag="PKN53_00225"
     CDS             complement(55369..55992)
                     /locus_tag="PKN53_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87412.1"
                     /translation="MIDYDYESSSVSVFISHFGRENFLFFLDKAYESAKNWESSWNQV
                     YEILMYQYWKFPEQRLILDENKRFYVSLSPLDYIYVDELTGIIDIENSMFEILGIAEC
                     AQYDKVLILPTKKYYSTHKKNAFRELFRINNICSKFGVSAFYFPIFQSGWKGKIKQII
                     EAENYESEFYIILFPSKASGYFLCRICPTILSKSKITEQDFNSIFKL"
     gene            complement(56166..56612)
                     /locus_tag="PKN53_00230"
     CDS             complement(56166..56612)
                     /locus_tag="PKN53_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87413.1"
                     /translation="MKQKLLFLLLVFPILTYSQESLKEYVHNEFNFRHLIDFSNKGNV
                     DSTYFYASKIINSKDKTFLPETYYRLAVLHNNKVKACANLLVLPTTSNLGKYFECKHK
                     HRNKSLNYLDKAVRFGLVFSDIKLEYDSLRYEKNRKFYESTYYIKS"
     gene            complement(56651..57643)
                     /gene="trpS"
                     /locus_tag="PKN53_00235"
     CDS             complement(56651..57643)
                     /gene="trpS"
                     /locus_tag="PKN53_00235"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOD87414.1"
                     /translation="METVLSGIRSTGNLHLGNYLGAVQNFVKMQNDYNCYFFIADYHS
                     LTTHPTPENLHNSVRQVLVEYLAAGIDPEKSTLYIQSDVPEITELYLLLNMNAYMGEL
                     ARTTTFKEKARQQPENVNAGLLTYPVLMAADIIIHNADKVPVGKDQEQNLEMARRFAR
                     RFNNMYSVEYFKEPQAFNFGKELIKIPGLDGSGKMGKSEGNGIYLADEASVIRKKVMR
                     ALTDSGPTEKNSEMPESIQNLFTIMKVVSTPDTVKFFEEQYANCEIRYGDLKKQIAED
                     VIKMLAPINQKIKEIDSNTEYLSKVAKMGAEKARENASKTLKDVKEIIGFRRFW"
     gene            57805..62034
                     /gene="glgP"
                     /locus_tag="PKN53_00240"
     CDS             57805..62034
                     /gene="glgP"
                     /locus_tag="PKN53_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOD87415.1"
                     /translation="MQNLNKPNYIFEVSWEVCNKVGGIHTVISTKALELAKNCDHHIL
                     IGPSIWRETKEHPEFIEDNSLFADWKHHLELKNTHIHIGYWNIPGKPKVFLIDFSKYI
                     SEKDSILAKFWEDYRLDSLAGQWDYIEPALFGYASAKIIESFVNFYLSVHDTIVAHFH
                     EWMTGTGILYLKSQMPQIATAFTTHATAIGRSIAGNGLPLYKNLSNYNGDILAGEFNI
                     VSKQSLEKLSAHNADVFTTVSEITAQECSQLLSKDVDFVTPNGFENNFVPQPAEYSEK
                     RKTARKKIIEVAQALMNYKYEKEPVIVATSGRYEFSNKGYDLFINALAELNKNTNLKE
                     EVLALILVPANNYGAVNQLYNILNNVEGDTNIVNNFLTHNIHDIEFDPISKRICEKQL
                     FNKAENKVKVIFVPTYLNGDDGIFDIKYYDLLPGLDLTAFVSYYEPWGYTPLESVAFG
                     IPTITTSLAGFGKWMQGVLDKNDKSVKVINRTDDNADEVVAEIINYFNFYLSLNQKER
                     ENLSKSAFAASDNALWTNLIKEYEKAYSFALEKVNDRQDEFVKQIPSRPISETYDKES
                     HSPQWRKLEVKTHVTDRFNALIAISLNLWWTWNKPARTMFKYIDPELWIEHAKNPVTF
                     LENVSISRLQELENDKYFTNLYDSVCKEFYEYIAKKEEKKAPKIAYFSMEYGFDDNLK
                     IFSGGLGILAGDFLKEASDTNTDLVGIGLLYRYGYFKQKITSIGEQNAEYIPQNFDKM
                     PIQPVRDDKNEQMKIMVYFPGRNVYAKIWKANIGRIPLYLLDTDVEENQEQDKYITSR
                     LYGGDIEFRFKQEMILGVGGIRALQALNIYPDVYHCNEGHAAFIGLERLRILRTRRNL
                     KFEEALEIIRASTLFTTHTPVPAGHDTFDENLMRTYMSHYPERLKITWDEMMRLGMLN
                     KGDKFSMSYLAANVSQEINGVSMLHGQVSKEMFKDLWKGYFAEENHVGYVTNGVHYHT
                     WTASAWQNLYLTTFGKEFLNDLSNQNYWSKIQDVDDEIIWDIRQKQRAKLVNFVKNKV
                     RRNWIRRYEDPKNLVAVTEKINENVLTIGFARRFATYKRGDLLLKNPERLARILNNPE
                     KPVQILFAGKAHPNDKAGQDLIKKIVQISKQPEFLGKIIFVEDYDISLAKHLVQGVDI
                     WLNTPTRPQEASGTSGMKAVMNGALHFSVLDGWWVEGYKEGAGWALPEKRTYQDQELQ
                     NELDTQTIYSLLENEIVPLFYNRDEKGIPHDWIKFIKNSIATIAPQFTTKRMIDDYFD
                     KYYNKLYQRSELMKPNLYEMASKIADWKKSVKRGWNDLEIVSVKFPDFDKHPLSVNEN
                     FTGEIEINLKGLSSDDIGVEVIVTDATTNGFTKIYAIHQAELVEVKNKIARYIVNSAP
                     KKPGFYNYAIRVYAKNDLLPYKQDSGLVLWA"
     gene            complement(62295..63134)
                     /gene="folP"
                     /locus_tag="PKN53_00245"
     CDS             complement(62295..63134)
                     /gene="folP"
                     /locus_tag="PKN53_00245"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008776467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOD87416.1"
                     /translation="MFIKVKSELLSVDTPLVMGILNLSKDSFYSGSIFPQVEDADKRI
                     EKLLADGADMIDIGAVSTRPGLTILKQEDEWQLLEPVLNLVNKKYSNIIFSLDTVYAE
                     TSKRAVKDFGIDIINDISGGEFDKKMFETVADLDVPYILMHMRGTPENMQEKTDYENF
                     IEDIIFYFSEKIAQLNSMGVNDIIVDPGFGFAKTVAQNFELLNNLERFKVFELPILAG
                     LSRKTMIWKTLGITPLEALNGTTVLNTIALMKGSHILRVHDVKEAKEVVKLYCNTKKT
                     VES"
     gene            63325..64014
                     /gene="tmk"
                     /locus_tag="PKN53_00250"
     CDS             63325..64014
                     /gene="tmk"
                     /locus_tag="PKN53_00250"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOD87417.1"
                     /translation="MAFFVIEGLDGAGKSTQLELLKDHFANIGKKTEYIHFPTTDSPI
                     FGDLVSRFLRGEFGGIKDVNPYLVALLFAGDRYNLKNKINSWLEEGKIVINDRYVFSN
                     IGFQCAKMNNEKEADELFDWIFNLEFEYFKIPKPDLSIFLDVPFSFTENKLKQTREGK
                     DREYLQGKKDIHEEDLSFQKSVRKTYLRAIEKYPNLIIRIDCSDENGHILSPESISKL
                     IIKEIEKRFTF"
     gene            64026..65570
                     /locus_tag="PKN53_00255"
     CDS             64026..65570
                     /locus_tag="PKN53_00255"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOD87418.1"
                     /translation="MKFLRNFSRLFTGIIFIFSGFVKVIDPLGSAYKFTDYFVAMHLD
                     FLNEGALVFAILMSIAELIIGIALVFNLLPKIAAWLLLLFMAFFTPLTLWLAVADPVS
                     DCGCFGDAIILTNWQTFYKNLVILAFTIIVFWQRKLFKPAYNLFNQWALTIAFTIASF
                     VLTLYCLYNLPIVDFRPYHIGANIQEGMQIPEEEKENVDIYESVFIYEKNGEQKEYSE
                     TELPDSTWTFVNADHKLVKKGYEPPIHDFTIEPIFVPGYSPEPENKYVNPWDLEFEFT
                     KDGETITCDLDSLPDQSWNFKKIIYNTKLNPDNLKLYFLNEEGEEIIANIKDLPDNNS
                     IFLDAEYIDTENENFLLKYGEDITNQVLEDESYAFFAIMTLLDEVNEKHLDKVAQISE
                     FCKNNNYKFYCITASNLEEVSAFINYHKPNYQFYNMDPITLKTIVRSNPGLVLVKKGT
                     VLNKWAAKNIPAPEQLHNDLTANSITKHQKAKNKYIYLTYIFGTLLFMSLFHGFYKYL
                     KTNRYI"
     gene            65589..66356
                     /gene="tpiA"
                     /locus_tag="PKN53_00260"
     CDS             65589..66356
                     /gene="tpiA"
                     /locus_tag="PKN53_00260"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOD87419.1"
                     /translation="MRKKIVAGNWKMNTLPAEGAELAKTICEHVKTLSQEEKYKIILG
                     VPFTHIDRVVNAIDLKFVKVSAQDCAMYDSGAYTGEVSAKMIKSINCEYAIIGHSERR
                     QYFGDTDEVIAKKLEMCYTSDLYPILCCGEKLEERENNSHFEVVEKQILNALSNVDAE
                     KMQKTIIAYEPVWAIGTGKTASPEQAQEMHEFIRKILAKKYGQEIANDTSILYGGSVN
                     SKNAQQLFDCKDIDGGLVGGASLKPDEFIAIINAIRK"
     gene            66353..67177
                     /gene="prmA"
                     /locus_tag="PKN53_00265"
     CDS             66353..67177
                     /gene="prmA"
                     /locus_tag="PKN53_00265"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOD87420.1"
                     /translation="MNYIEVKVNFNKEQTELSEILIAFLAEQGFESFYEENDSLFAYI
                     EEANYNLKNLDELLKSYKVSYSTKIIPQQNWNAEWEKNFEPTVVEDLLSIKAPFHDMD
                     FNTKYNIIIEPKMSFGTGHHATTWMMCKLIGEYDFENKDVLDYGCGTGILAIFAALLK
                     AKDIYAIDIDEWAYENTIENANRNNTPQIKAIQGDINSIPDKTFDFIFANINLNVLKK
                     DIPTLAKHLNNGGKIFFSGFLNNELVQIQKVCSENNLSFNKAIQKDNWTACVFNKI"
     gene            67180..71694
                     /locus_tag="PKN53_00270"
     CDS             67180..71694
                     /locus_tag="PKN53_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87421.1"
                     /translation="MKKLVFFFALILISNLIFAQAFPTEPDRFIKSFNSELGYTGEVA
                     STSKNLSKEFTTFWETDSLSFQEKEKFISTANNMAAKGCKGYPDFVCLADNRMWFSRK
                     AFDNSQYEIYEKGIFDILNAGKRPKLNDLSDYLLSFNYLLSKDIIAKNPRTHWKVENN
                     SFELKYDKGIKIQLSKVNLIGYQGVDSLKIYRTDAEYYPSQNLWKGVGGTVGWERVGY
                     SLDSIQAQLSNYQINTKNVSLTADSVTFNNVMYIKKPMLGRLIDKTGNLDNPKKSDYP
                     KFTSYNQHYYLKNFVPGIDYEGGFSVHGSSFIASGTKEEPATMLLTKSDSIYMEAKSL
                     AFYLDTKIIISDQCAISLHFGEDSIYHPYLTFKYYIHPRFLELIRSREGMNQVNYSNS
                     YHQINMDFTWMKWFVDKYKIEFTTMRTADSKEPNKALFESADYFRLERYRDIQKKDAQ
                     HPLAVITNFISSFWGSNDFQLNDLAKWMGYSPQQVVQMVLDLAYRGFLNYDPQSQEII
                     VYPNAWTFLQAYKNQKDSDVIQFYSVTDSNKTNAEFSLINFDLKIDGIHEIHLSDSQN
                     IRVFPNNKKITLQKNRTFTFDGTIQAGQFYFYGSNYKFDYNRFMIELNQCDSMKMVAE
                     TDFLDSDGTYKPAIVRNKLEQINGEFYIDEPLNKSGRQDFPEYPKFISNEKTYVYYDR
                     PDVYNGIYKRNNFYFEVDPFELDSIKGYSRDNLRFKGTLYSADIFPPIKETLVLRKND
                     FSLGFNTRTGPVGLPLYSGKATFWHEIDLSNQGLRGSGRIEYLAANMNPDYLIFFPKF
                     MEGHTPNFELKKQSAPIEYPKVEGRDNTLRWNVEDDDFMIKRDTSNFTMYEGKGSFEG
                     DLNLKSSGLEGDGTMFIDKAKIASELISFKQNVFDSDTANFDLYTINALNVDFKSDNV
                     KTHIDFIDRKGVFKSNGENTIWTFPKNKYMSEMNEMTWYMDKEEIAISADTDVLEKLE
                     KEGAFLEPDEWEAMFLEGPKFISIHPKQDSLYFVAPSARYDYINHIIYAEGVKFIKVA
                     DATIYTEDGNVTIEKDAVMQTLQNAKIIANSTSRHHTVYNASVNIYGKKNYNAFGDYD
                     FINSTKQPQTIHLTKIAVDASGQSYGTGTILEPDNFTISPYYKYQGDVTFYANNPELE
                     FNGAAKIIDDCDTTIFGWVKFKSFLDPEDIFIKIDSITYNINNTRLCSGLVLANTNQL
                     YPAFLTRKRNQYDQEIFSASGYLHYDQDDGKFIIAPKEKITETSLPGNLIRRHKSICN
                     VYGEGKFEFSKDFGLFKPNAIGNFMWYPETDTTEINVSMIADAYFSNAALKAMADKLN
                     STGGLSGLNVRDDVFEKALIEFLGTEKADQWFSNLSLGNYNKFPKELENKLIFTDLQF
                     YWHRALTSFVHYGPIGIANLGKEQVNKYVFGFIRIEKSRRGDVFEMLLEPDYSTWYYI
                     RYSAGTFTIISSDENFNNIVYNQKPNQREKTENGVFYQYGLGSSSYMKRFKKDMYKFF
                     KIDENINLDE"
     gene            71687..72967
                     /locus_tag="PKN53_00275"
     CDS             71687..72967
                     /locus_tag="PKN53_00275"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOD87422.1"
                     /translation="MNKANISICVIICIIIIQACVGVQPQNQTSRPSSYKYNFASRYN
                     PSSAEIQPEVRIFAKSKTEALVFYKIPTNEIQKTVNTIDDDARLIVKYTLRNADNFAI
                     VDSGTVVNMINLKQTNEEFNSYFTVNTPNQENYKIIISIYGEKANTGKRLLEDIELKQ
                     NNEAFFLPKILKNNEEEVLYTNFINSNNTYKLSSNICGPIVKIDYYKITEHVHLPPYY
                     IAPSQNVDATPDSTFTYSLNDSIKFNEKGIYFVREQNQSSGGLCLINIDDYFPEIKTV
                     ESMLEPLKLLTTNKEYKEIANSENLKQAIDYFWLTKSQSQKFAKEQIRVFYNRVALAN
                     KYFSENKEGWKTDRGMIYIMFGPPSIINISTNQEEWFYGEDPDVAGIFFLFTRIETAS
                     YNKSWILHRDANYQTVWAQALQIWRSGKIFTITN"
     gene            72972..>73660
                     /gene="rlmB"
                     /locus_tag="PKN53_00280"
     CDS             72972..>73660
                     /gene="rlmB"
                     /locus_tag="PKN53_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOD87423.1"
                     /translation="MENFIFGLHPVNEAIKSGKEIDKVLVKKNLTGNIALKLVSEIRQ
                     QGITIQYVPQEKLDKICKHNHQGIIAYVSPIKYQNLEEVIIKVQDENKTPLIIILDGV
                     TDVRNFGAIARSCECAGASAIVIPTSGSVRISDEAIKTSAGALYNIPICKVENLIDAV
                     LLLNQMNIEVVAVSEKAERLIYELDLTTACALIFGAEDKGISNSLIKRADKKAKIPLY
                     GKTSSLNVSVSA"
BASE COUNT        25934 a        11762 c        10851 g        25113 t
ORIGIN      
        1 gactgagtcc gaatattgaa agcaacgaag cagtttgaca cttatgagac accgcttttt
       61 tattttatta ttatatagtt ctaagtgtaa aaaactctaa taccctaccc gtttcagctt
      121 caatatcgtc ccatttatga aaatccaatt caacatgagc gacactacat gttggcatat
      181 tatcaatatt ggaatttgtc aacaagtttg caagatttgt taagccagga ttatgagcta
      241 ctaaaaatat atgtttagtt tttacatttt gttccattaa aacttccaaa aactctgttg
      301 tgctacacaa atatatttca tcaacaaatt ctatttcatc ttcatcatat ccaatttgtt
      361 cagcaataat ttcggcagta tgagcagctc tttctgctgg gctcgaaatt attaaatctg
      421 gttttggtaa atgttttgcg atatgttctc ccatcttgta ggcatcttca acaccttttt
      481 ctgttaatac cctgtcaaaa tcagaatcat ctgtgtcttg ctctgactta gcatgacgaa
      541 ttaatgttaa aagtttcatt tttgagaatt aaaataatct ggattttatc aaatacttaa
      601 atttctattt actatcaaat atttaaaagt taattaattt tgtgcaataa tatttatttt
      661 ttttgaaact acaaataaga attttgattt tttaaaaaaa ttttaactaa tgacagaccc
      721 atttgcagtt aaattttctg gactaacaaa tttcaaactt ccgtctttat ctgtagccat
      781 caaaatcatt ccagcagatt cgataccacg caattttcgc ggagccaaat taactaaaac
      841 acaaacttgc ttacctatta tttcttctgg tttataatat tcggcaatac ccgaaactat
      901 tgttcgctta tcaattcctg tatcgactaa aagttttaat aatttatcag ctttaggaac
      961 tttttctgcc tctaaaatgg tagcaactct tatatcttgc tttacgaaat catcataagt
     1021 aatttcttct ttttgcggac ttacagtttt tgccgaactt tcattcattt cttttgtttt
     1081 tgcaagttta tttaattgtt cttcaatttt tgcgtcatca ataatttcaa ataataattc
     1141 tgcttgattg attttatgtt caggctttaa caaatcaaat ttcaaaacat cttcccaatc
     1201 tgttttttcc atatttaaca tattgcgaag tttttccata gaaaatggta aaaatatttc
     1261 acccaaaaca acacttatag cacatagttg aagagaataa tacattatcg ttttaactct
     1321 ttctgaatct gttttaataa gtttccaagg ctctgtgtcg gctaaatatt tatttcctat
     1381 tctagaaatt tgcataaaat cttttaaagc ttctctaaac ttatagtttt ctatattttt
     1441 ttctaagtct gattttattt ttaaaatttc ctcgcgaatt tctttttctt tatcagtaat
     1501 ttcgcttgga acaggaattt ttccatcata atatttatga gtaagcacaa gtgctctatt
     1561 tataaaattc cctaaaatag caactaattc attattattt cttgcttgaa aatctttcca
     1621 agtaaaatcg ctatctttag tttctggagc atttgcacaa agcacataac gcaaaacatc
     1681 ttgtttgttt ggaaattcct ctaaatattc gtgcaaccaa actgcccaat tacgcgaagt
     1741 actaatttta tcgccttcta aatttaaaaa ctcatttgct ggaacatttt ctggtaaaat
     1801 ataacttcct tcttgtttaa gcatagaagg gaaaataata caatgaaaaa ctatattatc
     1861 ttttccgata aaatgaacca atttagtttc tgcgtctttc caatattttt cccaatcagg
     1921 tcttagttct tttgttgcag aaatatatcc aataggagca tcaaaccaaa cataaagcac
     1981 tttgttttcg gcttcatgca aaggaacttt tacaccccaa tctaaatcgc gagttactgc
     2041 acgtggaaaa agtccagcat ctaaccaaga tttacattgc ccataaacat ttggcttcca
     2101 ctctttatgc tcttctaaaa tccattgttt taaccaattt tcgtatttat ctaatggtaa
     2161 ataccaatgt tttgtttctt tcaaaattgg tttattgcca cttattaccg aacggggatc
     2221 gattaactct tctggactaa gcgaagaacc acaattttcg cactgatcac cataagcagc
     2281 ctcatattca cattttggac aagttcctat aatatatcta tcagctaaaa actggttttc
     2341 ggcttcatca aaatattgtt tgctaatttt ttcgataaac tcgcctttgt tatacaaagt
     2401 tttaaaaaat tctgcagctg tttcatgatg aatttggcta ctagtacgac tatatatatc
     2461 ataagaaatt ccaaattttt taaaagattc tttaatgata ttatgatagc gatcaacaac
     2521 ttgttgtggt gtaactcctt cgttacgtgc tttcaaagta attggtacac catgttcatc
     2581 agaaccacca ataaaaatca catctttatg tcttagtctt aaatatctaa cataaatatc
     2641 cgcaggcaca tacacacctg caagatgacc aatatgaaca ggtccatttg cataaggcaa
     2701 agcagttgtt actgtatatc ttttaaattc tttcatcata aaatttttgc aaatttaaaa
     2761 aaaatgggat aagcctttaa tataaattct atttttacac tatatcctct cgataattca
     2821 agcactcttc aactatatgc ttcgaataac ttaaatgttt aaaaacattt tcatctgcta
     2881 gattcaacaa ccttcttctt tcaaaaacta tattttcaat caaatcaaga tcaagattaa
     2941 attttccacc ataattatca aaatcttctg gatagaacat agcgtgtatg aaaggggtaa
     3001 gataatgcgt ataaaactta tcataacgtc gtgtatctat ttgttttcca aaccaatttt
     3061 ttccacacgc acactgtcca aatatgataa tagtatttgg attattatct tcgaacggta
     3121 tccaaccaac aacatccaac cctttatctt ctgtactttc gtctggaatt aaatcaataa
     3181 ttctatcgtc acgcgcataa acattaatat ctttacctaa tttaataatc ttctcttttg
     3241 ttttcccttt atatttagaa ttactaccaa acccaaaaac ttttgcatta cttggcaaat
     3301 acgctttcag cactaattct gataatttct caaagtcatc agtaatgaac tgttttatgt
     3361 ccctaaatga actcaaagaa gaagcaatta acaaataaat gtacaatttt tgttgctccg
     3421 atagttctga cttgtctata acttttatcc ccttattttt atcaaaaata aagggataat
     3481 tatcaccata gattaatttc cttttttcta aatattcaaa atatgtgttc acatatcctt
     3541 cagctttatc atttatttga gaatcaagca aaccatattc accatcttct gggtgctcaa
     3601 cttcagcttt aacgccttca tcaaatagtc tatctttaat atcagactga gagacaaagt
     3661 caggtgaaac aagactaact aattcaatat agtcagcata gtattgattt ttatactgtg
     3721 ctgatttagg tggctttatt gtgaactttt taaaaagttc actcatcttt ttattaaaat
     3781 tcgtcatctc taagtccctt tgctgatttt atttttttta ttcgttgata aatctcgttc
     3841 aagtcttcat ctaagctact ataaaaatgc acaaccttaa cagttaacaa atcagctttt
     3901 tccatgtgcc tgatagaatt ttttattgca ttttcaaatt gaacatcaaa ctcatctgta
     3961 aactcaattg caacaaaaag gttttcacca tctctaaatg cctttaaagc ttcaggcgaa
     4021 gctatgactt tattaagagt atttaaattt tcactatctc ctattatttt atttggcaat
     4081 tctttattaa acaaccaatt agtccattct ttcagatttg tatactgaac attttccgta
     4141 ggattatctt tattaaaatc aacccctaaa aactcggcaa tatttgaacg gcttaaacta
     4201 tcggctaaat agttaaaata aaatgtagtg tcatccaatc cacttatcct ataaaaacca
     4261 ttgttttcga ttatttcata tagtttgtat cctgctaaaa cgcgaacaac ataatcttta
     4321 cgactaccta tcatttttgc tatttctcta cttgctgaac taatagaata tgaagggaaa
     4381 taatcatttt taagttttgc taaataacga gctttttcta ataacttcca cgattttatt
     4441 cctgttatat gtctatagcc aagatatttt aatatctcat ctttatcttc aaatatcaaa
     4501 catggcaagt cagtaggttt atgctcagcc tctctaatca cttgctcaat cttgctttta
     4561 taaacttctc caagttctgg attatgaagt aatttcactg ctgatagtct tcgatttccc
     4621 tcaacgacta aatacttatc cccttcatca ggaacaacca ataattgttc tccctcaaaa
     4681 aaatcatttt tactaatggc taacattagt tcaattaaag aagcatctga taaaagaaag
     4741 ttaataattt ccttttcgct tttattactc attgatttgg gtaagcgagg atttttcgaa
     4801 tctaatatta aattcgtaac aggaattttt tcatgtttta cattagacat actgtattaa
     4861 attttatttc gtttccaata ttgatttact tcaaatagtt ttgctttatc aaattctttt
     4921 atatttgaaa ttgcttgtcc tttctcgatt tttgacatac tttgaactat cttatttgtg
     4981 tctttacccg ataatccaag gaatttttcc attgcttttg tattgttttt atctttaata
     5041 tcaagtaaaa aagcaatctc acacattgaa gaaaaatcaa aacccttttg attgtattct
     5101 tctattccct gcgacaacaa tacaattgat acaccttttg agcgaatttc tcgcaacatt
     5161 ttctctaaaa tttcttcata ttttttttct ttgaaaagaa catgtgcctc atcgattaaa
     5221 attacataac gtaatgattt tatgttgttt tcactgggac tattgtccat gttcataaac
     5281 gtattatata tgtaattgat aattaaaaac aaagatgtaa aacgaacaga tgttggtaag
     5341 tccccagaga gtgataagta aagattttta tttaaaaaat tagattcatt tttacggtcc
     5401 tctaaaaaga ttttataacg gcttaaatca ttcatcattt ctgtcaatgt atcctgctta
     5461 tcataagttt cttttagttt ctcattgact tgtgaaattg taggatatgt tccatctcgt
     5521 tgttcaacaa atgcttccga tgttgcctct ctaagttttc ctctttgttt tattcctaaa
     5581 ttagaatact tacaaacaat gtctacaaac ttgtcaattc ccatattttt atcactttca
     5641 ttaacattat caataaaagt aagcggatta acagggaatt gtgttctagg aacatctaca
     5701 aattcagttt ttgtatcttt aaaaaacttc tcgtaagttt ttatatcttc ttgcttcaaa
     5761 cctttaaaat ctaaataaat atagtttaca tgcccgtttg acttatcata aatttgtgaa
     5821 ataaactcaa gtgcaaattg tgtttttcct gtacccgaat tacctgcaac tgcaatgtga
     5881 caattgttat attttgtatt atcgttgaca ctcagatata ctggctcttt tgtatcaata
     5941 gtttttccaa cttctaattt gataatgttt gaaaaataag acttttctat gttttgatga
     6001 gcgtttctaa catgttccgc cgaaactgta acatctacaa gatgtcttat ccctttatca
     6061 atatgttcat tcaaaaagtc gaaaaaagta tattcactgg tatcaaaaat cttatccaaa
     6121 agctctaacc cattgtcaat atgaagttta atgtattttg gaatattttc atctgttttg
     6181 taaatttcat aatgttgaca aattaaagca atgtaaaaat cacgatgtcg agagtcaaaa
     6241 agaatatgat ctttatactc ttttcctttg gaatcagttt gactgaataa atgatttata
     6301 ttaaatttta cacctttttg caaagaataa gcaaatgcga ttcttgcaat tacattttca
     6361 ggagttcgat ctccaagttt tggtgttaaa cgacgaatta tatcctgact tttttcagaa
     6421 gtttttatat taatttgcat tgctaatctg attatatttt gagaaaaaat tgttaggttc
     6481 ggtttctaaa aactcagaac tatctgacga tgtattatta attaaaaatg tcctacaaac
     6541 atttgactgt aataaattat attcattttc ggtcaactct ttatgtaaca gaggaaaaat
     6601 cacgacttgt tttgcaacat ttggataaaa atgtttgata atattttcag catgttgatt
     6661 gtcaaacttt tgcattgggg aatctataaa aacaggaaaa tcaatttcag attctgaaac
     6721 aagtgaactt aacaaagctg aagcatacaa ctgtctttct cccattgaaa gtccactttt
     6781 gtcaatttct ctattattct ctccatataa gacaatatca atatcttcac tagtgaggat
     6841 gtcaacatca acttgttgca caaagtcttt tttatgtaaa agtcgctgta aagaatttag
     6901 catttttgtt tgtaatgatt ctttcttttg ctgtttaaat ttcaatatga actcttccaa
     6961 agtttttatc aattgtttcg ttttttgttc tttttcggaa tattgtgcag aatcatctat
     7021 ttgtttgcga atagccgttt ggtcttgtct atgttttttt aacgcctccc tccagtcatc
     7081 tattttctga ttcaacgaac ctatttctcg ttctttttca ctaatttgtc catccaacac
     7141 ttcttttttg tccctcaaat cagaaatatg taagtcttgt gcgttttttt cagcatctct
     7201 aattttacga tttatcgaat caatctgatt tttaacatat gaatactcat tatttaaact
     7261 tttaaattta acttgaaagt tgttttttat ccctacaatt aactggttga cttcatttgt
     7321 ctgactatct gaaaaatcgt gtaatatttt catattctcg tcaatttctt gtatgtctga
     7381 ataaaagtat ttttttacca atttttcaaa ttgcttaata taaaagtctg ctgttttaat
     7441 atctggaaca aaatttagct tttgtttttc agattcaatt tcttggcgaa tcttcgtaaa
     7501 tttctctttt acatcatctt gtttgtattt ctgttctgat aataatcttt cattttctat
     7561 ttgctctacc atatttgcca aagtttcacc tgctaatgca aatggtataa aatcaaaagt
     7621 ttcttttagc tgttcttgta gcttagactt ctgttcttcc aatttatttt tctcatctcg
     7681 tagattcaaa agttcttcta gactcattgt atcacctgct tttacaagtt ttaaagaaat
     7741 atcgtcagat tcgtattttt gagacctgat ttcttctgta agatcattga tttttatttc
     7801 acaattttcg atctcaagtt ctacattttt tatatctgct tcaagttgat tcaacttttg
     7861 tttatcttca ttttttgctg actttttacg gtattcatca agtttatctt cgagattatc
     7921 ttttaatgtt tcatattttt gaatacctaa tacttcagaa tatgctctac ttaactctct
     7981 tcgttggtct ttctgattta tttctgcaag agaagtaatt ttttcagcat caaaaaagaa
     8041 aaattttgca atagcaattg gtagaataaa ctctctaatg aaaatctctt cgcccttctg
     8101 attatcttga gacaagtttt caataagttc ttgtggttgc ccacttataa aaacctctac
     8161 tatatcagaa gtgcctgtaa caatattata gctacgtttt acaataacat cacacaaaac
     8221 attctgaaca ttaacctctg aaaaagtaat cataacagaa aattcagttt ccccatcttc
     8281 agccgccttg cgattcatgc tatccatgat gtatctcgta taattacctt tatcctctat
     8341 ttcttttttg tacattgcat caacttgttc catgtttttc ccgtacaaac accaaacaag
     8401 tgacattaaa aatgttgttt ttccaaaacc attttgacca ctcacaatta caatattttt
     8461 ttcacattga ggcgataaag taattcgatt tacacccttg taaattctga aattatttag
     8521 ttctacttcc tttataaaca ttacaccgtt tcttttttga gatatttttc aataacttgc
     8581 tccatttcat cttttaatcc gcgtttacga tttaaaagtg ctttttgttt ttgtaactca
     8641 agaagttcat gaattaattc aaaatcatga ctttcgtcat tgcaaacaga cttcaacaaa
     8701 tcaatttcct tttgtatttt ttcatctaaa actttcatat ctaattcttt attataaact
     8761 tcattgtaaa tttctgatac agttttataa cttgtaagct ctctgtaatt tttatttggc
     8821 attgctaagt ctcttcgcca aattacctga atagcaataa gttctgagtt cttaataagt
     8881 tcaatacttg gatcaaactg ttgaatgtat ttttgaactt ctaaaacttg ttttagcaac
     8941 ttactcctat attctattgt ataattaccc tgattgtgtc catctgctgt tactgctttt
     9001 tgtccattcc gccttgtagt gtccctgttt tccgatatat ttctttgatc aaacatacta
     9061 cttcttaaat ctaaaagtgg cttcaaccac tctttaccat tttctacttg agcagttagt
     9121 gacttatctt cttttactac cgtacaagtc caacatccaa agcgactttg cccgcaagaa
     9181 cggtgtttat catcagaaac cattgtagga cactcataat catcagcact tgcatcagca
     9241 taaattttaa ataactctga attgtttgaa ccccaaggcg atgatattgc attgattata
     9301 taccaaactt cttctaattc taactcctta attggagaat aaacaaaagt attaacttga
     9361 tttgggtgct ttgttaaacg tttccctttt acttcatgtt tttttattgt attagcacga
     9421 tttacgcttt cacttctgcg agtcccaact aaaatgatag cttcaccatt tttatctact
     9481 tcttcaacaa tataacgtga tgttggtttt attttcatct tgtcagtaca ccaacgaaaa
     9541 gtattattag gaactggata accacgtcca actacattta cccaaaaagt atcttccaaa
     9601 cgaggaactg ttttttttac tgaaatcggc atatctcgct gtacagctgt ttgttctatt
     9661 tttttcaaca catcatctac atattcctta atgattggat tttctaccaa agtatcgtta
     9721 caaataacat gtatttctcg ctttctcaac tcttttggta tttgctccat agccaaccag
     9781 accaattgta aaagtgcggt tgaatctttt cccccactaa aaccgataat ccatgatctt
     9841 aaattatctt ctattagata ttggtcttta agttcagata ttatataatt tattcgattt
     9901 gacattttta tttttatcag attacaaaat taataaaaaa ataagatttt gtgccattat
     9961 ctctattttt ttaaaatttt ggtttgtaaa tcattttgta ttttaataat tagacaaaaa
    10021 atactttaaa caaaaaagcc attcagtttt tgaatggctt atcttaaaaa caatttttca
    10081 ctatttaatt tttgccaatc tagcttttac tgttgttgtt gatttttgga atataaatgg
    10141 tactcctttt gtttgagttt catactgtgg ataaaaaacc acatcataac ctgcaccatt
    10201 cttttccatc aaatcccaaa ttgcataagc ttctgaccct gcaataggca aattgccaat
    10261 gataggaaaa ctgaaaggaa gtgcctgaat tgatcctgtt ttactaacaa ataagtggct
    10321 ccagtcaata ccaaatacct tggtttgagc tcctcgtcca acaacttgtt ctgaaaaagt
    10381 aaaatcgtct tttttaaact caacatagtt gttaggagtt ttcatggttt tcttggtgta
    10441 attacaactt gaaaaactaa ttgcaaaaat cgcaatcaaa gaaattaata aagcttttgt
    10501 cctcataata atttttttta actttattaa aagtttcagt tttcaagata attttatagt
    10561 attttatttg agaacttaag tattatggat agtaagattt ttttttctaa aaataattgc
    10621 aataaagaaa aatagctggc aaaaatacca gctattcttt tttaatgaga gtgattgatt
    10681 ggagactcga actccagacc ccctgcttaa aaggcaggtg ctctacctgc tgagctaatc
    10741 aatctcaata atcccttttt gggactgcaa agatatattt aaagattcat attttccaaa
    10801 ttttttgcaa ttttttttta ctttttcaat accgcactct ttagttgctt gacttttaat
    10861 aaattacaaa gaaaaaaata tttgtttttt taaagcaact tgttttataa atattcgttt
    10921 tataataaaa ttaaatatta tatttacatt ttgaaaaaat ttatttattt tgcataatat
    10981 tttatattta aaacaaagcg cacaatgaaa aaaattcact tattgttagc aagccttggc
    11041 ttatttttat tagtaagtaa caatgctttt tctcaaccca gttatggtgg acaaccttta
    11101 agttttacta aaaacaatcc tctgtccaat gaaattgatc atataacaat taacccacca
    11161 gaaatgcagg caactatagc cgaagcagat tatcttgaac aaaacgatgg actttataga
    11221 attgcaacaa ttattccagt taatttaaat atggaaaatt ctggtacttg ggattattta
    11281 gaagacggta caaaaatatg gcgacttcat ataaatatgg aaggagcaaa aggtcttgca
    11341 ttatactata aggattttca cttgccaaaa tattcacaat tatttttata taaccaaaat
    11401 aaaaaacaag taattggtgc ttttgacgat agaaataatc caaaaatgtt ttctgatttt
    11461 tctacagaaa ttattcaagg agctgacaca tatattgaat acatacaata tcctcaggca
    11521 acagaagatg ctaatataga tatttataac atatcttatg tttttagagg agtttcacaa
    11581 ttaattggca aatacgaaaa cagcactaaa gaccctggaa atcttggtgc ttcgggttct
    11641 tgccaagtaa acgtaaactg tcctgaagga gctgactggc aaacacaaaa aagaggtgtt
    11701 gccgaaattt attgtgttca aggttttttt ggtggtcttt gtacaggttc tttagttaac
    11761 aatactgcaa acgacggtac tccatatttt ttaactgccg accattgtgg tggttctgct
    11821 acaaatttta gtaattggca attttatttt aactatgagg ctagtggttg tacaaatcct
    11881 ggaactgcac ctgcctacaa tactgttact ggagctgtaa aaagagctag aggagatgaa
    11941 aacactggtt ctgacatgtt gcttttagaa ctaaatacag atcaggcgac tttagctggc
    12001 tacaatgctt attataatgg ttggtacaga ggaacagatg ctagtccaag tggagtatgt
    12061 attcatcacc caagtggaga tattaagaaa atttcaacat atactgctcc tttaacttct
    12121 gctacttggc aagctagttc tccagcaaat gcttggtggc gtgcaaattg ggcacaaaca
    12181 caaactaact ggggtgttac agagggtggt tcttctggtt ctcctatttt taataacaat
    12241 aaacttattg ttggaacatt aactggtggt gcttcgggtt gtggtaacgc tgctgctcaa
    12301 caatatgatt tatacggaaa attcgattat cactggcagt caaatggtgg cgcaccagcc
    12361 gatcaattaa aaccatggtt agatccaaac aatactggag cagaaacatg tcctggattt
    12421 gacccaaata acacaggcgt agttgcacct gttgctgatt ttgttggaaa tcccacaact
    12481 gttgttgttg gcggaacagt taactttaca gaccaatcta caaacaaccc tacatcttgg
    12541 gaatggactt ttgatggagg aacacctcct tcgctcacag gaccaactgc tcaaaaccca
    12601 actattacct ataatactgc aggaacattt accgttacaa tgacagccac aaatgcagca
    12661 ggtagcgaca caaaaacaag aacagactac attactgttg tggaccctgg tactttaagt
    12721 gcaaactttg tagcagacca aacaacaata aatgttggag gtacagttaa ctttaccgac
    12781 caatctgtag gtaatattac ttcttggagt tggcaattcc ctggaggtaa tcctgcaaca
    12841 agcagtgtgc aaaacccaac cgtaacttat ggagctgctg gcacatttaa tgttacacta
    12901 attgttagtg atggtactga ccaagacgaa gaagttaaag ttggatatat tcatgtaaca
    12961 gataacgctg ctggtcttca tgctagtttt gttgcttcta gttataatat tattgctggt
    13021 gagtgtatta attttaatga tcaatctact ggcagtccaa actcttggtc atggtcattt
    13081 cctggtgcaa cacctataaa ttctacaaac caacacccaa ctaatatatg ttatccaaat
    13141 gctggaattt acgatgtggt gctacaagtt caaaatgcag atggtcaaga tacatatatt
    13201 tgccaagaat gcataactgt taatcccgac ccaaacagtc ctattgcaga atttgaagca
    13261 gacaaaagag taattcctgt tggtggagta gtacgtttta caaatctttc cgaaaacgga
    13321 ccatttaatc aatgggcttg ggaatttgaa ggaggaactc ctgcagttta caacgattcc
    13381 acaccaccac caattgcata tttacaagtc ggtacttata atgtaacttt acgttgtcgc
    13441 aagacaaata atgttcaaga tattaataca aaagttgatt atattactgt tattccaaag
    13501 gcaacaactc caccaactgc taactttaca gcaaactaca ctgttgttca acctaacgac
    13561 ccaataaact ttattgattt aagctctggt aatccatacc aatggacatg ggagttccct
    13621 ggttcagacc aaggtactag caatcaaaaa aatccaatgg gaataacata tccagaggag
    13681 ggtacttata cggtaaaact aactgtaggc aacaactttg gaactgatat aatgattaaa
    13741 gagatgtaca taactgtaag cgtagatgat ccttgcaccg acgagcctac agttgccttt
    13801 aatgcaagtg ctagattaat cccagcagga ggaagcgttc aattctttga cgaatctgaa
    13861 ggttttccaa cttatcacac ttggtctttc attggtggtt ctccatcaag cagtaacgaa
    13921 ggctcgccaa gctctcctat acgatatagc actccaggca tttatgatgt aagcctaaca
    13981 gttagcaact cgtgtggctc aagcactgtt gtaaaacata attatattta tgtatttagt
    14041 agccctgttg gacaatattg tgatactatt acaacaataa aacctggcga agttgttgag
    14101 ccaagagtac cagatggtca atggggatat ttagctggac acaatggact taaaataaga
    14161 gaatatgcaa atcgctatga tgctcacaca tttagcgaag tatcggcagt tttagtacct
    14221 cttaaacaag taaaatatga cgctactgat tcatatgtga agttttttgt tgcagacgct
    14281 aattactcaa ctccagattc gattttgggt gaaaagaaag ttttcttaca tcaacttaca
    14341 aaaaatgaaa ctaatcttat taaatttgaa ccaccaatta gtgttaatgg tccattttat
    14401 gttggataca aaattaacta tccagatgaa gacctagatg gattctctga tgatatgctt
    14461 gttgtaggaa tacctatgcg tcctaatgat cctgcacaaa atcaacttta catgaaaaaa
    14521 ggaagcactt ggtacactcc aaatgatttt aaatcaataa acccagcaag ctctaatttt
    14581 agttcagcac ttcctttaaa acctattgct tgtttagttg atatagaaga aattattgca
    14641 gaaaacgaca taaacatcta tcctaaccca actaatggag tagtaactat aaaaataaaa
    14701 gacacttatt actcaagttt tagttttgaa atttacgaca taatgggtag aaaagttgtt
    14761 agcgaaattt ataaaaatgg atttgatgaa tttactactg atttaagcaa ctatccagaa
    14821 ggtttgtaca ttatcagaat gaaacttgga aatttaaatc ttaataaaaa gatattgtta
    14881 acaaaataaa agttgataca actaaaaaat taaaacctct gattatcttt gcatagtcag
    14941 aggtttttta taaccacatg ttaaaactat agtttcaaaa aaaataaaaa atgaaattta
    15001 tatacttttt aatatttaat tttttagtat tatttctttt aaattcatgt aataatcaac
    15061 acataaaaac tgaagaagga caaactattc caaacaaata cgcaacagat tttgaaatac
    15121 aagaaatttc tgatggctat attttaaaag ttaaaaatcg ttttgaccaa acaaatagcg
    15181 gtacttacaa ttattattta tcatatgaac cagaatcttc tcctaattca aatgttacaa
    15241 tacctattcc agtttcaaaa gttgcgtgtt tgtcaaccag tcatatagcc tttatttcaa
    15301 ctttaaacaa aacttcttct ataaaaggca taagtacacc agaattagtt tgtaattcag
    15361 atgtatcaaa actttatgca caaggaaaaa tttataatat tggatatgat aatcaaataa
    15421 actatgaaaa aatcatttca atttctccag atgttgtatt tgcctttggt gttgacaata
    15481 ctagcgtttc gaaatttcaa aaactaacag acctaggaat acctgtggtt tttgtcaatg
    15541 attttaccga agctaaacct ttaggtagaa gcgaatggat aaaatttttt gcttgtttct
    15601 acgacaaatt aaattttgca actgaatatt ttgacagcat tgaaaataat tatctaaata
    15661 taaaatcatc tattcctcaa accacagaaa aaccaaaagt attggtaagt ttgccttgga
    15721 aaggcacttg gtgggttcct ggaggaaatt ctttttttgc aaactttatt aatgatgcag
    15781 gcggagaata tatttttagc aacaataatc attctgatag ccaaggttta aatattgaag
    15841 aagtgtttgc aaaagctaaa aatattagtt tttggctaaa tccaaataat gtaaacacta
    15901 aaactgaaat tttatctata gacaacagat tagagttttt tgaaccatat aagactgcaa
    15961 aaatttacaa caataattta aaagaaaacc aagctggtgg aaatgatttt tgggaatcag
    16021 gaatattaca ccctgacata attttattgg atttaaaaac aattttttat caagattcaa
    16081 caaatataca caatctatat tattataaac aaataaatta gccatgcgta aatgaaaaaa
    16141 accaaactta caataaagtt tattttatta agtgtttttc taattttctt aggactctta
    16201 gaccttttat caggatccat tcaaatttca gtatcggata tttttaatta tattttttca
    16261 agcaatacag aaagtgaatt ttatttgtta ttaaaagaat ttcgtttccc aagaatgagt
    16321 atggctattt tagctggagc tagtttatca atttctggat taattatgca aaccttattt
    16381 agaaatcctt tagctggacc agatatttta ggaattagta gtggagcaag cttaggcgtt
    16441 gctattgtcg ttcttggtgg aagctatttg tttggaaatc atttttacca cgcatctaat
    16501 tacataactt taatagcagc aggaattgga gctgcatcaa tattaggatt aattctaata
    16561 atctcttata aacttcgaga tatactttca atattaatta taggtatatt attagctgga
    16621 attatttctg caatagtaaa tattttacaa tattttgcca caggaattaa cgtaaaatct
    16681 tttgtagtat ggacaatggg ttcattaaca gcaagttctt ctaaagattt attactcgcc
    16741 tcgcctatta tattgatttt tgtattgtta gctttttcgc tatctaaaaa tttaaactta
    16801 atgttactag gcgaaaattt tgcaaaaact atgggagtaa atataaaact tttaagaatt
    16861 ttaagtttta caatagttag catattaaca ggagttataa ccgtttattg tggtcctata
    16921 ggttttgttg gaattgctgt tcctcatatt agcagatgga tattcaacac ttccaatcat
    16981 tttgttctaa ttccagcatc agcactttta ggtggtattt ttatgttgtg cggagatatt
    17041 ttaagtaatc tctttacatc aaacggcaat ttgcctatta atgcgataac agcaatcctt
    17101 gctgctccat tcattatttg ggttgtgttt aaaaacaaaa gaacatttgt gtaatggaaa
    17161 actcaatctt aaatataatc aatcttaaat tcggatacaa gattccactt tgcgaacaag
    17221 aagtaaatct atctgcaaac attggagatt taatatgcct tgtaggatta aacggaagtg
    17281 gaaagtcaac atttttaaat acaatttctg gaattttaaa acctatttct ggaaaaatta
    17341 ttatagacgg acaagatgtt aaaaagctaa gtttaaataa taaatcaaaa ttaataagtt
    17401 ttgttccaac aaaaattgat tttactgcaa atcttacagt tcgtgaatta gcagaaatgg
    17461 gaagaagtcc atatacaaat gttttcgata aaaaatctga agctgacatt aaattagttg
    17521 agaaaaactt aaatgatttt gatttattaa aattacaaaa ccgacaactt tggagcttga
    17581 gcgatggcga aagacaaaaa gcaatgattg caagagcatt aatccaagaa acaccactta
    17641 tgattttaga cgaaccaagt gcttttctcg actatgaaat gaaacatcaa ctaatgagaa
    17701 aactaaaaga aataagcaca aacaaatgtt taattttttc aagccacgac atagaaacat
    17761 ctacaaaatt cgcaacaaaa atatggttta ttaataaatc aaaatttgaa gttttaagtc
    17821 ctatggaatt tatagaaaat cactataaaa aatcaattta attctacaaa aaatcatata
    17881 aaaaaattac taaatttgca cactttttgt tgtgaaatcg aaacaacaaa tttattgaat
    17941 gaaacacata aaattaattt tcataatttt cattgcttca tgtaatttag gagcttttgc
    18001 acaaaaatat accattagtg gttatgttac tgacaaagct tctggcgagc atatatttag
    18061 tgctaatgtt tacgatatgc gtacaaagca aggcactaca acaaatgaat atggatattt
    18121 tagtttaaca ttagatgccg attctgtaaa tctaagtatc tcttttgttg gatacgcagc
    18181 ttttacaagt gatatatatt taacatcgga tttgaattta aacatacaat tagatccaaa
    18241 cataatgttg gcagaagttg tgattttaga taaaaaatct gatgaaattg taaaaagcac
    18301 acagatgagc atgatagaaa tgccaattca tcaaataaaa gctttgcctg ttttgctcgg
    18361 cgaagcagat gttcttaaat ctttacagtt aatgccaggc gttcaatctg gttctgaagg
    18421 aagtagcggt ttgtatgtta gaggtggcgg tcccgaccaa aacctaatac tattagatgg
    18481 tgttcctgtt tataatgcct cacacttatt tggtttcttt tcggttttta atactgatgc
    18541 tatttctaat gtaaaactta taaaaggcgg ttttccagct cgctatggag gaagattatc
    18601 ttctgtttta gatattagaa tgaaggaagg aaacttaaaa gaatatcacg gcgaaggctc
    18661 tattggaaat gtttcttcaa aatttacttt cgaaggacca atagtaaaag ataaagcctc
    18721 ttttgtagta tcagcaagaa gaacttatat tgatatttta gctgctcctc tcgttctagc
    18781 tgtaaataga gcagcaggaa attctgacaa atttagagca ggatattatt tctatgatgc
    18841 aaatgcaaaa ctcaactata aaatttcgga caaagacaga ttgtttttta gcatttatac
    18901 aggaaaagac aaagcctaca caagatcaaa acaaagttac atttacaacg acaccaccaa
    18961 aacagaagca attaatttta atttgaattg ggggaatatt accagtgcat tacgctggaa
    19021 tcatgttttt cataaaaaac tttttggcaa tcttacagct gtatatagta aatataaatt
    19081 tgaaactatt cagaaatatg aatttttatc tggtaaaaca atagaagaag aatattattt
    19141 caaatattta tctggaattg acaatgttgg agcaaattta gactttgact atagacctct
    19201 accaaaacat tcggtaaagt ttggtgctaa ttatttatac cacactttta atcctggagg
    19261 ttcaagtatt tcaatgaagt ttggaggaaa tagtataact caagattttt ctaacaaaaa
    19321 aatatatgct aacgaatttt atgtttatgc tgaagactca tgggatatag cagccttatt
    19381 aaaattaaat tttggagttc attattcggg atttgctgta caaaaaagat tttaccaatc
    19441 aatacagccg agagcttctg caagattttt aataaatgat aaaatgtcgt taaaagctgc
    19501 ctactcacat atgtcgcaat acatccactt attaacaaat actagcatag gtttgccaac
    19561 agatttatgg cttccatcaa ccgattcggt gcgaccagag ctttcaaagc aagcagcctt
    19621 aggctttgtg tataatattg acaacaactg ggatttgagt gttgaaggat attataaaac
    19681 catgagtaat ttaatagaat ataaagaagg agctggattt tttcaattag cacaaaactg
    19741 ggaatcaaaa ctcgaaattg gcgaaggata tgcttatggt gcagaagttt tattaagaaa
    19801 aaactatgga aaactatcag gttggcttgg ttacacactt tcgtggtcgt ggagaaaatt
    19861 taaaaatatt aattttggag aacctttccc ttataaatac gacagaagac atgatatttc
    19921 gcttgtgctt atgtatgagt ttaacgaaaa agttgatatg ggacttactt gggtttatgg
    19981 aagtggaaat gctgtaactc ttggagttgc acgatataat agcttgaact tagattatta
    20041 taatcctggg gagtattatt atggtgaaat agaatacttt aaaggaagaa atgggtatag
    20101 aatgcctgct taccatagat tagatttaag tataaaccta aagaaaaaaa caaaatgggg
    20161 aagtcgaaca tggagttttg gcttatataa tgcatatagt cgccaaaatc cgttttattt
    20221 atactttgga tacgacaata ataaaagagt acttaaacaa gttagtttat ttccaattat
    20281 tccatctgta agatatgcat tcaaatttta aaaacttaaa caaatgaaag caagtatttt
    20341 taaatatatt ttactatttt caatattagc aagtttaatt tttagcattg cttgtgaaaa
    20401 aacaattgat atagatattg aagactcaaa accaaaaata gtactaaatg cagaaataaa
    20461 tcccgactca acaattaaag tacacataag tcgtagcaga catattttag atattgcaaa
    20521 cctttcaact ctcaacgatg cagaagtaaa attattcgaa gacgaaactt ttattggaaa
    20581 tttaatttat gatgcaagca ttagttgtta ctgcataaat tacaaaccaa tacaaggcaa
    20641 aaactacaaa tttgttgtta gccatcaaca atttgaaaca ataaatgcaa catctcaaat
    20701 tttagaaaaa ataaaaccca tatctattga cactagcaga tccttcggac aatacaacga
    20761 agaacagata aatataagca taaagtttaa agatccagca aatgaaaaaa actattatat
    20821 gttaaaactt cgctctaaat ataaggctga aatttgggat cctgatttaa taaccataga
    20881 tactatttat gaaggacctg atactacaat aatagatatt tctcaaggtg gatattacta
    20941 cgctgatgcg attgacccta tgcactttag ctcaaatgat ttgaatttag aagacggcga
    21001 ttacctgaga agtttagtga ttgcagatga gttttttgat ggtaaagaat acgttttaaa
    21061 aatttctctt gatgcattct ctttacctta tacctctgat actacagtag tatatattga
    21121 tttctatgca attagccaag attattataa atacttacgt tctgaaaaaa aatatcaaaa
    21181 ttcttcaggc gacccttttt cagaaccagt tatggttttt actaatgtcg aaaatggttt
    21241 gggaatttta ggttctgctc atgcccacac tgattctatt ataatcacag gaagatacgg
    21301 cggtcattat gatgaataac gaaacaaaat tagatattac tttaactgtt ttagaggtaa
    21361 aagacgaaag tctaaaacct tatatctata attattggct atttataagt tctgaaaaac
    21421 ccaatttatg tgcagccatt acagctcccc cgcttggctt tcccatgttg caatttgtat
    21481 ttggcaattg cgaaagttat tatcaaatta gaaataaaaa tactacatct attataacag
    21541 gaatttactc caaacatgca actttgacac ctgctcccaa aatgggtttg gtaaccataa
    21601 actttaagcc atatggcttt tataattttt ttggaaaaac cccgccaaat ggtacacaag
    21661 acagcattga tggatatgat atttttaaaa aagaaaattt aaattctgca atagaaaata
    21721 tttccattac agaaaccaac gatgagctag taaactgttt ggaagatttt ttatgcaaaa
    21781 atcaaaaagt aaacgttaaa aaaaacgact actttgatag cttagttgat aaaatttttg
    21841 ccaaaaaagg tttgatagat atagataaac tcatcaacga aaaattttct ataagaacct
    21901 ttcaaagata tttttctaaa gttctaggag tttcgccaaa aacattttct agaatattac
    21961 gacacaaata tattatacag ttaatgtaca caaaacctaa tataagttgg aacgacctat
    22021 gttttcaagg tttttatttt gaccaatcac attttattaa agattttaaa gacttcacta
    22081 aatgtaaacc cacagaatac gactacttaa aaacatctat tatttacgaa ctgtttaaat
    22141 aatttgaact tttatgtaaa tacttgtttt ttgtcgtttt tttacaaact tttgtcaatt
    22201 tttagagttt attttgtgta agcatttcat aatagatttg taagttctca cttttctcaa
    22261 agagccttga ttttccccaa aaaaattaag gcttttttct tttaataaaa attaaatatt
    22321 ttaagttaaa acattttgat aattaagctt ttaaagctat attgcaacaa aaacgcatta
    22381 tgttaaatca aacagatatt aattatttaa aaagttttgg aatcattgaa aactcaattg
    22441 aagaacaaat taaactatta aaaaaacatg agtctttttt aaacataaat aattttgcca
    22501 gcacaaaaaa tggcattaca agaattgaag accctcaatt ttatgtaaat gtatttaaca
    22561 acaattcaaa aaattataaa ttggcaaaat ttgtacctgc ttcgggtgct gcaacaagaa
    22621 tgtttaagag atatatcact tacaaattag atccaaatct taaaaactta aacgatggag
    22681 ggttttattc tgtaaaaaat ggattagaaa acattaaaaa ttttgctttt tacaaaaaac
    22741 taaaacctct ttgtactgaa ccagaaaatt tagaaacagt agttgaaaaa attctttatt
    22801 ctggtttgaa ttatgcaaat ttacccaaag gtttaataga ttttcataat tatcctgatt
    22861 ctccacgaac agcttttgaa gaacatttgc acgaagcaaa cctacttgtt caagatcaag
    22921 aaaattttaa tattcaccta actgtatctc cagaattttt agaaaatttc caatcaaaac
    22981 ttaaagaaat caatcaaaaa acaggatata aatttaattt aagtttttca gaacaagaac
    23041 aatcaaccaa tacaatttca acttatctaa atggcgaaat tgtaagagac gacaatggca
    23101 acattatgct tcgtccaggc ggacacggtt ctttattgac aaacttaaat agcctcgaca
    23161 gtgatattat ttttataaaa aacatcgaca acctaactca cggcgactat ttagaagaaa
    23221 ctattttgtg gaaaaaagtt ttagcaggag tattaatcga aattgtcgag aaaatacaag
    23281 aaatttactc aaatctcaaa aataatccaa caacagaaaa cttaaataaa gctaaagatt
    23341 ttttagaaaa acacacaaat gccatttttg ataattctga ggatttaaaa acagaagttt
    23401 taaattatat taatcgtcct atcagagttt gtggaatggt gaaaaatact ggcgaaccag
    23461 ggggcggacc attttgggtg aaagataaaa atggaaaaat aagtttacaa attgtcgaaa
    23521 aagcacaaat aaatcttgat attcctgaac aagctcaata ttttaataat tctactcact
    23581 tcaaccctgt ggatttggtt tgctatgtaa aagaccctga aggacaaaaa tttgatttaa
    23641 aaaactttgt tgacaaaaac tctagttttg tttccgaaaa aactcatcaa ggaaaaacca
    23701 taaaagtact cgaacaccca gggctttgga acggttcaat ggctgattgg ataagtgttt
    23761 ttgtagaagt gccacttatc acttttaatc ccgtaaaaga actaaacgat ttgttgagaa
    23821 aagaacacca gccaaaagaa taatttatga ctaaggcaaa aatttcaatt cgttttttcg
    23881 acgatcgcga agtccgtgct gtttgggacg aagaaaataa caaatggtgg tttaatgtta
    23941 ttgatataat tgctattcta aacgaacaag gcgactatac caaagcaaat aattattggc
    24001 ggtggctaaa acgaaaacta aaatcagaaa acaatcaagt tgtgagtgct actcacggtt
    24061 tcaaatttgt agcagctgac ggcaaaaaac gcttatccga cactcttgac agtgctggag
    24121 ttattgaatt agcaaagagc tttccaaaca ctaatgcaat aaaattttta gattggtttt
    24181 tatatagtga taatactatt gacggacaaa gtaagaaaaa agcttacaca ctttttgaaa
    24241 gcaatttaat aaacgaaatc gaagtaggca caacaaaagg cttacaacaa attcacgcct
    24301 atctttttgg agggctttat gattttgcag gacaaatcag acaaaaaaat atctcgaaag
    24361 gtggatttaa gtttgcctca gcccagtttt tagaaaacac tttacaacaa attgaaaaaa
    24421 tgcctcaaaa tacatttgac gaaattgtag aaaaatacgt ggaaatgaac atagcacacc
    24481 catttatgga aggcaatggc agaagcactc gcatttggtt ggatatgatg ttgaaacaaa
    24541 aactaaaact ttgtgtggac tggagcaaag taaacaaaaa cgactatatg aaagctatgg
    24601 aaaaaagtgt taccaatagc gaaacattaa agagtttgct aaaaaatgta cttacccatg
    24661 aaattaatag tcgcgaaatg tttatgaaag gcattgatta ttcatattac tatgaagatt
    24721 aattgaaaca ttcaaaaatt ctcaattaat tttaatttac agtctgcttt tgctttggga
    24781 taatttttcg gcattaaatc tataggacaa agaaaacaca tgagaataaa gagctatact
    24841 ttgatttcca atgtcggtaa atgcataatc tattttcaag ccaaacaaat tcacaccaag
    24901 tcctatatta ggttgccaag aagttgattt agtattatca aactctggaa tttgttgaaa
    24961 attatttact ccagcacgag caaaaacaat atttcgaaac gacacttcca atccaaacga
    25021 tggatctata ctaataagat ttgttttaat aagagtattt ctttttccgt ctgtagttgt
    25081 aagcattccc aactcgccat agacagaaaa atcatcactt atattatata aatatcctgc
    25141 acctaaaagc aagcgaggca aagtaatttc caatccattt tcaggaattt cgtttccagt
    25201 agcttcaaaa acatcgcgta aaagctcttg attaaatttc caagcattaa aagttgtagt
    25261 tatatcgcga aaatttgcac caaacttcca atttttataa tgatacattg cagaagcatc
    25321 tagaccaaag ccccaagcat tagcaaattc gccaacattt cgtctaatta tttttgcatt
    25381 tccaccaaca cttaatcctt caatatttaa tttgcgtgaa taagaaaata aaaatgcata
    25441 atcagcaaca gaaaaagacg aaattctatc atatcttata tttccatcgc tgtcgatgag
    25501 gtctaaagtg tttggaatat catcaactcc aaaacgaatc atgcttaatg ctaatgcaga
    25561 tttatcatca attttataag aagcacccaa aaaatcgtat tttgcaattc cagcaaaata
    25621 ttcggagtgc atagcaccaa tttctatttt tttatctaat ttatacaatc ctgcaggatt
    25681 ccaataagtt gcatttatat catcacttga agcaattaca gaatttccca ttcccaaagc
    25741 ccttgctcct accccaatgc ccataaattc attgctatat ttaggagcta tttgtgcttt
    25801 aagactacaa ctaaaaatta ataaaaacag taatatattt agtctaaaat tcatttcaaa
    25861 ttattatttc aacaaaataa cataattatt tgctatttat tgtaagtttt accaaaaaac
    25921 ttcaatattt ttgtgtaaaa ttaaatattt ttttaaaact tacaaaaatg aaaatatata
    25981 aatccatacc atctttaata acttcaggaa atttaatttg tggtgcaatt gcaatatttc
    26041 ttgcattttc gaacaattta gaatttgctg tttattttgt attgctagct tctgtttttg
    26101 attttttcga tggttttgct gctcgaaaat taaatgctgt ttcagaattt ggtaaacaat
    26161 tagattcact tgctgattta ataagttttg gttttgctcc tagtgctatg atctatgttt
    26221 ttattgaaaa taatgaaata ttgcctaaaa acttttccta tctttctatt ttattggttt
    26281 tatttgcagc atatagacta gcgaaattca acacagacga aagccaaacc actgaattta
    26341 aaggacttgc aacacctggc atggctttat ttattatggg aattgtttat ccaaaaagtt
    26401 taggattaat ttcaacagaa caaatatttt taaataagat ttttataatt gcgtgtctta
    26461 ttattctccc tgttttgatg gtttctaata ttagattttt ttcttttaag attaagaaat
    26521 ttagttttaa aaatattgtt tggcaactag ttttattaat ttttggaatt attttgttaa
    26581 taatatttgg aataaatgga ttagcatatc taataatttt gtatcttgca ctttcaattt
    26641 taaaaaattt aacaactaaa aaaactatat aaaatgaaat ttttagcaca aatcaacatc
    26701 atgccacaaa aggctttatt agacccacaa ggaaaagcag ttttaaactc tgcaaaaaaa
    26761 attggatttg atactgtaaa caatattaga gttggcaaac acattgaaat agaagttcaa
    26821 gctaaaagcg aacaaaatgc tgccgaaata atagatgact tgtgtaaaaa aatactaata
    26881 aacccaattg ttgagtctta cgaattcaaa ttatttaaaa tttaaaatag ttttgcaaat
    26941 aataaattag tttgtatatt tgcaagctca aattatggtg agtgtagctc agttggttag
    27001 agcatcggat tgtggttccg agggtcgtgg gttcgagtcc catcattcac ccaaaaaaag
    27061 cttcggtaaa acgaggcttt ttttatttac aattcttttt taaagaaatt gtttgtattt
    27121 ttgcgaactc aaatcatggt gtttgtagct cagttggtta gagcatcgga ttgtggatcc
    27181 gaaggtcatg ggttcgagtc ccatcattca ccctaaaaaa gcttcggtaa aacgaggctt
    27241 tttttatttc ttattctttt attttaggaa aatatttgta tttttgtgat gtatcaaaat
    27301 aaaataatta tgacaaaaga ttttagaaaa tatgcaattc acaatagagg aattaacggt
    27361 ttagtatttg acgaatacac aagtatttac aacaattata tttctccaac aatcatagaa
    27421 gaaagaactt taaacattgc aactatggac gttttttcaa gattgatgat ggatagaata
    27481 atttggttgg gagctcctat tgatgattat gttgcaaata ttattcaagc acaattgcta
    27541 tttttagaaa gtacagaacc taacaacgat atacaaattt attttaatac acccggcggt
    27601 tcggtttatg caggacttgg catttacgac accatgcaat atattagttc tgacattgct
    27661 acaatttgca caggaatggc agcatctatg ggtgcaatac ttttatgtgc tggagcaaca
    27721 ggaaaaagat cggcactaaa acatagcaga gtaatgatac atcaaccaat gggaggtgct
    27781 tcgggtcaag tttctgatat tgaaattaca acaagagaaa tattaaaact taaaaaagaa
    27841 ctttacgaaa ttattgctta tcattctgga aaagattata aaactgttga aaaagacgct
    27901 gacagagatt actggatgac tgctgaagaa gctaaaagct acggaatgat tgatgagatt
    27961 ttaattaaaa atgtaagttt ataattttaa atatttacaa accaatttat ataagttaga
    28021 atggaaaaat gttcgttttg cggagctcca aaacataaaa atgatttgtt catagcagga
    28081 atgtatgcaa atatctgcga attttgtgtt gagagagcat acgaaattgt gcaggaagat
    28141 agattggcaa acaaaaaatc agaattttct ttaaaaagct taaaaaaacc taaagaaata
    28201 aaaaaatatc tcgaccaata tgttataggt caagacaatg caaaaaaagt attatcagta
    28261 gctgtttaca atcattacaa aagaatagca caaattataa aagaagacga tgtgcaaatt
    28321 gaaaaatcta atatacttct tgttggcgaa acaggtactg gtaaaacact tttagctaga
    28381 actatagcta aaatgttaga agtgcctttt gcaatagttg acgcaacagt tttaacagaa
    28441 gctggctatg tgggcgaaga tgttgaaagc ttacttgtaa gattattgca agatgctgaa
    28501 tatgacgtta aagctgccga aagaggaatt gtatttattg acgagattga taaaattgct
    28561 cgcaaaagcg ataatcccag tattactaga gatgtttctg gagaaggcgt tcaacaaggt
    28621 ttgctaaaac ttttagaagg ctctgttgtt aatgttccgc ctcaaggtgg acgaaaacat
    28681 cccgaacaaa aaatgatagc tgttgacaca aaaaatattt tatttatttg tggtggagct
    28741 ttcgatggaa taaacagaca tattgcaaaa agaataaata ctcaagtaat tggttacgga
    28801 aaagaaaaaa gagaagaaaa aatagataaa gaaaattttg ttcagtacat aattcctgac
    28861 gacattcgca aatatggatt aattcccgaa attgttggtc gtttgcctgt gttagcacac
    28921 ctaaaccctc ttgataaaga tgcgttaaga agaattttaa ctgaacctaa aaattctata
    28981 atcaaacaat acataaagtt gtttgaaatg gacggcatta aattaacttt taaagaagaa
    29041 gctcttgatt atatagttga acaagccgtt aaatttaaac ttggagcaag aggattaaga
    29101 tcaatatgtg aatctattat gttggaagca atgtttgaat ttccttctaa aaaagttaat
    29161 gaatttgttc ttgacaaaaa atatgcagaa agtaaattag accaaactgc aataacaaaa
    29221 ttcaaatctg cttaaaaaat gattttttta gctttataaa ttttaacggc accgcacaaa
    29281 agatttttta cttttatatc agaaaaacct gcttgttcta atttgttagt caaatcaaca
    29341 ttattagcaa aatcaacaat agatttggaa agatatttat aggcttgctt gttttttgat
    29401 attatttttc cataaaaagg gattattaaa ttcaaatata atttaaaacc aaatctacac
    29461 agaaaattct ttggcaatgc aaattccatt attgcaagac aagcattttt ttgagaaatt
    29521 ctataaaatt ctttaaaagc ctcatcaaca gaagcaaaat tcctaacacc aaaagctaca
    29581 gtaatcaact gaacactatc atcttgtaga tttatctttt ctgctttaga aattaaaatt
    29641 tcagccgttg gtacttttgc ttttgcaatt tccaacatat tttttgatgg gtctaaagca
    29701 atatattttt gtgattttaa tttttgaagt tccagcaaca aatcccctgt tccacttgct
    29761 acatcaagca caacatcgta atttctgtca gaaacaaatt ttacaaactt tcttcgccaa
    29821 actttatcta aatttaacga caatctatga ttaagtttat cataagtttg agcaattgca
    29881 tcaaacattt ggggtgtatt ttctttttgc attaacatat aaaaagtggc gaaatcaaaa
    29941 caaatttgaa aacatatgaa acaccacttt agggtttatt ttttcctttg ttgttgcaac
    30001 ttttgttgtt gagcacgttg agcttcttct atgcgttgtt gccatttaga ttttggcttt
    30061 gcaggctttt tcttatttgt ttccatttga gccaacaact tttcctcatt aataataaac
    30121 ttttgtatta ttagagtttg tagtattgtc aaaacattag caaggaaata atagtagctc
    30181 aagccagaag aatatttatt aaaccaaatt atcatcatta ctggcataaa gtacattata
    30241 aatttcattc ctggcataga attattttga ggctgatttc ggcttgttat ccaagttgaa
    30301 acaagcattg aggctgccat taataacgta aacaaagaaa tatgatttcc ataaaaagta
    30361 gatattatag gaatatgtgt tgaccattca actattgcat cgtaagatga taaatcgttt
    30421 gcccacaaaa agctttgttg acgcaactca attgaagctg ggaaaaatct aaacattgca
    30481 attaaaatcg gaaactgaag aagcataggc aagcaaccac ccataggact aactcctgct
    30541 ttcttgtaca aagccattac tgcttgttgt ttttccattt ctttgccctt aggatatttt
    30601 tcatttaagg catcaacttg aggtttcaaa acccgcattt tagcagaaga tagatatgac
    30661 ttataagtta atggagctaa acctatttta acaataagtg ttagcagaaa tattatcaag
    30721 ccatagttat ttataaaacc gccaagccaa ttaaaaatag ggataattat ccatttgttt
    30781 acccagccaa aaattcccca accaagtgga actaattttt cgagtttaat gtcatatttt
    30841 tttaatttcg agaacttatt aggaccaaaa tacattctga aacctacttt ttgtttttct
    30901 gcattttcga gtggaataga aaaagtagag ctaaaagttt ttaaataatt acccgaaggg
    30961 tcatctattt tctcggcgtt tacaacaggc gaagacaaag cctcatcagc aataagaaca
    31021 gaagagaaaa actgttgttt ataagctatc caatgtgcag aaccactagt tttaaattca
    31081 tcttttgctt ttgctgcatt tagagattct atgtctttgt ctatttttaa atatattgtt
    31141 gtattattat tttcccaatc tatacctctt tctaagcgtg gaactttcac ttcccatctc
    31201 atattggcat aagagtttac aagagtattg atttcatttt gcaatccaac aaaatttata
    31261 tcaaaatcta acatataatt atcgggataa acggtatata aaaactcaat atacttatca
    31321 tctgcggctt ttaaacgcat agaaagtttt tcttctttgt ccacaacttt aattgtatct
    31381 tcctttgttg aaggagcaaa atacatatca ttagtaataa tagattttcc agcaacaaac
    31441 agttctaagc caaaagttga caaagaatca tttttatata atattagtgg atcttgataa
    31501 taagttttat aatctttcaa ttctacagaa gcgattttac ctccgagatt tgaaactttt
    31561 ataatcattt tattgttttc gataattgta aacccgtcat cattactatc aaaagcattt
    31621 gcaaaaatgc catatttatc ttctagttta agagcattta tagaatctct ttcttcttga
    31681 gacaagtttt catgattttc aattttttct tgttctatag ctttgtcgat actatcttgc
    31741 atagcaattt gaactcttgc aagagaatct tctttaattt tggcttgagc tatagagtct
    31801 tgcatttttt gcctagcttc tatttgctct ggagttggct tagtaagaaa catatagcca
    31861 accaacaaag ctccaattac caataatcct attaccgtat ttctatccat tattcaaact
    31921 cctaactttt attttgtctt ttaaaatcaa tagctgcatt aataaaatct ataaaaagag
    31981 ggtgtgggtt tagaacagta cttttatatt cgggatgaaa ttgaactcca acaaaccatg
    32041 ggtgttttgg aatttcgatt atttcaacaa gatctatctc ttcattaatt cctgttgcaa
    32101 ctaaacctgc ttttacaaaa gcatctttat atttattatt aaattcgtag cgatgtctgt
    32161 gtctttctga tattttaaga gatttataag cctcatgtgc tattgaacct tttactaatt
    32221 tacaagaata cgagcccaaa cgcattgtac cccctttgtc gtttaccatt ttttgttctt
    32281 ccattaagct aataactgga tttttgcagg ttctatccat ttctatagaa tttgcgtctt
    32341 cgagtttaag aacattttga gcaaactcaa caacagcagc ttgcattcct aaacaaatac
    32401 caaaaaatgg aatattattc tcgcgtgcat attttactgc tgaaattttt ccttcaaagc
    32461 ctctatgtcc aaaaccaggt cctacaaaaa ttgcatgtgc atctttgaaa aattctgtac
    32521 tagaattatc atttacatct tcagcttgga tataatttat atttactttt gtttctaaaa
    32581 aagctccagc atgtataaac gactcattta tagacttata cgcatctttc aaatcaacat
    32641 atttaccaac aaatgcaaga tttatagatt gttttgggtt tttagttttt ttcaaaaatt
    32701 ctttccacgc tgttaatttt ggtttttcgc ctatagcaat tccaaaatga gataacacaa
    32761 attcgtctag tttttccttt tgcatttcta aagggacttc ataaattgtg ctcatatctc
    32821 ttgattcaat aacagcttct ttttgcacat tacagaaatt tgcaactttt actttaacat
    32881 catttgttaa aggctgttct gttctaagaa ctaacatatc gggttgcact ccagcctcta
    32941 gcaatgtttt tactgaatgt tgagtaggtt tggttttcaa ttctccgctt gctgctaaat
    33001 aaggcactaa agttaaatgt ataaccatac atctattttt aaattcccat ctaaactgcc
    33061 taacagcttc aatataaggc aaagattcta tatcaccaac agttccgcca atttctgtaa
    33121 taacaaaatc gtactttcct ttagtactta aaagttttac tcttcttttt atttcatcgg
    33181 taatatgcgg aattatttgt acagtttttc caagatattc gccacgtctt tctttattta
    33241 taacttcttg ataaattctt ccagtagtaa cactatttgc ttgtgaagta taaacattta
    33301 aaaatctttc gtaatgacca aggtctaagt cagtttctgc accgtcttcg gtaacataac
    33361 attcgccatg ttcaaaagga tttaaagttc cagggtcaac attaatataa gggtctagct
    33421 tttgaattgt tacagtaaag cctctagctt gcaatagttt agctaaagaa gctgaaataa
    33481 ttccttttcc caaagaagaa gttacaccac cagttacgaa aataaattta gtattagcca
    33541 tcacaaaatt ttaaaaagta aaacttaacc caagactagg cataattgga agttggtcca
    33601 ctcgattata gtctattcta tcaaaataga aaatatttct tctattataa acatttgtta
    33661 ctccggcatt aagctctaac ttagtatatt tattaaaagt tataatttta tttacagtta
    33721 agtctaatct atgataatat ggcaaacgtc ctgtattaag ctcatcgtaa ataattccta
    33781 acgaaccatt tccttgccaa taaggatagt ttagactatg ataaaaatcg acttcttcgt
    33841 aaaatcctgc agtttttgta aatggaaatc ctgaacctaa attccatctt gcactaatgc
    33901 tccaagattg ttttttacct aatttatatg tagttactaa atttatgttg tgtcttctat
    33961 cataatgtgg actatataac tgcacgccat catcacgttt tacccaacct aaagaataca
    34021 caaaccaaac ataaagatta tttttatcat attttaataa aaagtcaagt ccgtaagcat
    34081 aaccagtttc gattaagaaa tcttttttca aataatctgg tttagttcca tttattggaa
    34141 tatcgttata tattttattt ctattaattg ttgaaagctg agaaaaattc tttatgtatg
    34201 cttctacatt aaagtctata tgattagtta catctacttc tgcaccaata attaaatgtt
    34261 ctgatttttg aagtttatgt cttaactctt cgcctttgaa cgagcttggt aaatttaagt
    34321 cgccactaag gaaaccataa aaaagattaa caacatctct atcactattt gcggcaacta
    34381 aattttgaga gtacagacca gcagctaatt taaacctaag tctctctgtt gcgttatatt
    34441 taagtcctaa tctaggttct aaagatgctt cactcataga cgcatagtat tgtagtctaa
    34501 gtcctggctc aataagtaaa cttccaagat taagtttata ttttgcaaaa gcagccaatt
    34561 ctgttgtgtt ttcttcttgt ttcaactctc taccgataga gttgtaataa ataaaatctg
    34621 tattaaagcc caaggtttct acaccccaaa caaactgatt tttaccaaag aaatacaaga
    34681 agtttaagcc aaaattatat cctctaattc cacttttact aggtaaatta tcaagagttt
    34741 gcaatccaat tttatagtct gaataagaaa agttagattt tatcataaca gtagaaccta
    34801 atggtacaac agaaacattt gctcctattc cataagaatc ccaaccgagt tggcttaaac
    34861 cttgataact aactctatcg ctaaatttga atccaaataa atttatctta cttccttcgg
    34921 gtgagcttaa agatactttt ccatacaagt ctgtatagtt aaatggtagt cctttgccat
    34981 cattaatata tgtgtataat aatttagacg attgttcgag ataagaagtt tttccagaaa
    35041 acaaataaga agcacttact ttattttctt tatatttaac aataggtcct tcgagtaaaa
    35101 cttttgaccc aaagctactt gccgaaactt ttcctgtaaa acgtcttgaa tttccttctc
    35161 gtatagataa atccattact gaagaaattc ttcctccata gtcggcatta aatcctccag
    35221 tataaatatc tgcatttcta ataatatcag aatcgaacac tgaaaacaaa cctatagaat
    35281 ggaatggatt ataaatagtc attccatcta aaagcacaag attttgaata tttgagcctc
    35341 ctctaatata tagttgccca ccttggtcgc ctgtaaaagt aacaccaggc actacctgca
    35401 aatactgagc tatatctggt tctccaccta ctgacggaag ttgttttatt tgagttggag
    35461 aaactttaac catagaaatt ctggtttgag ttttcatttc ctgcctttct gccgagacta
    35521 ctgtttcgcc aagaacaaaa gcagattttg tcatataaaa attcttagag tataattttg
    35581 cactttctat gcgcaaatct tcaactatac tatcatagcc cacataagta attataattt
    35641 tataatctcc aataggaatt tttggaattg aaaaatatcc attcaaatct gttgctgctc
    35701 ccaaatttgt tcctttaata gaaacatttg caaacataca aggttctcca tcatctttgt
    35761 catatacaaa accttttatt gttccttgtt gagcataaag tgttactggc aaaaatgaaa
    35821 atataaaaaa tgtatttctt attattttat gcatcgtttc cgtctttgtt ttttagttcg
    35881 cgttctgcca aatctatagc tttctttttt tctttaagag tttttatttc ttcttgtgct
    35941 ttttcaattt ttttattaaa atcttttacc aaactttcag ttccagaagc aaaaaatccc
    36001 atattatttt ctatttgcaa tatttctgca tttaattctt taattttttg taaaatacga
    36061 tttttctctc ttgcaagttc gtttaaagca ccacttccct taatagattc aactttgttt
    36121 ataaaattac taatttctaa agcagattta ttaagtttta attttttata caactcatta
    36181 atagctccac gatattcgtc atacaatcta tctctttgtg aaatttcaac aaagccaatc
    36241 tctgtccatg tgttttggaa ttctttaatt ttattaacgt tttcgctttg gtcgtcaaga
    36301 ggtttatagt ttttaatttc ttcgataaga gcctctttct tagctaaatt ttctttgtgt
    36361 tctgtttcaa tatttttgaa aaactctgat ttagcattaa agaaatggtc gcaagcagct
    36421 ctaaatcttt tccatatttg ttctgaattt tttcttggaa ctggaccgat ttctttccat
    36481 tgtttttgta aatccaaaaa cattctagtt gtttttttcc aatctgtact atcttgcaag
    36541 ctttctgcac taagacaaat gtctgtcttt ttttgcaaat tatcttttag ctcagcattt
    36601 atttcactaa aaaattcatt tttagcttca aaaaacttat tgcaaccagc tctaaacctt
    36661 tcaaaaacct cattattaac tgctgttggc accattccta tagttttcca aattttttgc
    36721 aattctaaaa ctttgtctga aagttctgcc cattctttac catttgttgc agtgttttca
    36781 actaaaatac tttcaacttt ttcgcaaagt ataagttttt gtttatagtt agcttctctt
    36841 tcttctttta gtttttcaaa atggtcttga taattttttc ttattttttt gcttatatca
    36901 gaaaatctat cccatagctc aacacgttta tcgttaggaa caggacctaa ctctttccat
    36961 aattcgtgat aatcctgcaa ttttctataa gaagcaacta catcggttga taaaagcaat
    37021 tcttctgctt tttcgcacaa ctcaattttt tgttctaaat ttcgtttaaa atcaaaatct
    37081 ctggcttctt tatttagctt aactaaatca taaaaccttt cgatttgaag ttgataatta
    37141 ttccataatt cgtttgaaac ttctgctgga actggtccaa ttttcagcca ttcttgttga
    37201 agttctttaa attcgttaaa agttttattc atcgactctt cgccatttgc taaaactttg
    37261 attttttcga tgatttcttc tttcttttta agattttcta atctttctgc ttcttgttgt
    37321 tttgctagtt cagcacgttt cttgccatat tcagcccaca attcttttaa ataatcttcg
    37381 tttgggtctt ttggcaaact aacctctaat tcatttcctg ttgcctctat aatctcttga
    37441 cgcaaagcat cttgttgtct tctcaaattt ctataaaaca atcttttgag caaatcaata
    37501 tctttacgag tttcttcaat attagattcg taaataagct gtttaagttt ttcaatgatt
    37561 tcttcattat tcaaattgct aaaatcattt tctgcaggaa cattatcgtt atcaacatct
    37621 tcatcgtctt cgtcagcatc aatttctgaa ataaaaacct catcagtatt tattttttta
    37681 tcttcagttg atttgatttc aactttttct attgttttat tttgctctgt ctcaacacta
    37741 ccattttctg cattaatctc aactttttca acattattgt tgtcttggtc agtgtttttt
    37801 gtcgtatttt cttggttttc taaaccattt ttagcatttt caatcatact aaatctctga
    37861 taattaaacg tttaacaaat tattaaatta atgttataaa acttacgaag gtatatttta
    37921 tataaattag aaacaaattt ttatcgattt tttctatttt gaaaatttat tttgcgaaaa
    37981 taatacaaat tttctataaa tttttaaaaa atgctgcctt ctctcgctca gatttatgtt
    38041 gcaacaaaat aattattatg gtatttgaat aaattggcat tgttgtaaaa tcaacgcatg
    38101 tgttgattct acaacatgct atataattta ttaaaaatca aactttcgac tttaccttcg
    38161 gtgagctcgt aaactcggtt cgacttttaa cacttcaact acgttcagtg catcgccttt
    38221 aactgttttg ttcttcttct tcagatttaa caaaaatctt ccctatcccc tttccgatat
    38281 tttctatagc ctcaatacct tttgtcaatg gattatttgc aaggtcgtca tactgagcta
    38341 tagagccttc tatttgctgt gggaaaatta caattctgtt atattcagaa taatatcttt
    38401 ctattttgct gaaaatattt tcaaaatatg gcacataaga aaacgaattt cttcttgccg
    38461 atgcaacaat cataaggtcg tctttttcaa tatatcttgc taaaattaaa aaatcttccc
    38521 attcgctaaa ctcgctaaac tcgattttta cattaaattt attctttttt acgagagttt
    38581 ttaggctttt atcaacttct tcggtgccaa aatgaagtat ttgtgccgac agttccgaag
    38641 caagtttaag cactttttct gtccacaatt taaaaccttt ttctttattt gctaaatgag
    38701 gtgtaactaa aataatcctt ttattggcaa caaaagcatt ttcaagagaa gtaacaaaaa
    38761 cgtttttatt tcccgaataa ataattttat tcactctctc tccagctagt ttattgataa
    38821 tgccagcttt accgggccaa ccaaggatta gaatatctgc catcatttct tttgacacgc
    38881 gtgaaattcc actaacgaca ttatagtcaa tgcttgtaat tgcgtttact tctacttcgg
    38941 cagcagcgcc atattttata aaatcttcta atttctcttt tgtttttaaa agattttgct
    39001 cagcattaga atcattttga ataattgata aaagagatat tggtcttgat gattttaagt
    39061 tttttatcaa tactgcaaac tctaaaagct ttggcacaga agctaagttg gcaactggta
    39121 taagtatatg ctcggcaaat ttattataag ctttttcacc aatttgacta tcttcttcgg
    39181 ttaaagccaa agttttagct gctctttcgg ttacaaatga agctattata caagaaacta
    39241 aaattagaat aattgtaccg ttaagaatat tgtcgtctaa aattcctatt ctatagccca
    39301 ctaaaattac tgccaaagtt gcggctgcgt gtgaagttga caagccaaaa attaattgtc
    39361 tttgagttcc agaatatcta aaaattactt gagttaaaaa agctgctatc cattttccaa
    39421 aaacagcacc aacaatcaaa actccagcaa caatagctgc accccaacct tgaacaacaa
    39481 cgcttacatc tactatcatt cccaccgaaa ttaaaaagaa aggaataaac aaagcatttc
    39541 cgctaaattc tattctattc atcaaagcag atgtttttgg aattatagga ttcagagcta
    39601 aaccagcaac aaaagcccca ataattggtt ctaaaccact tatttcggct aagaaagctg
    39661 caaaaaacaa aactgataaa gcaaatataa aatgagcatg tttttcgctt tctaattttg
    39721 agaaaaacca tctcgcaact tttggaacaa ggaaaaacat gattattaaa aacaaaacaa
    39781 aacaaattcc caatctaata aaaaactctt ggtctaagct accttgtgta tttccaatta
    39841 taatggctaa aatcaacaaa acagcagtat cggtaaaaat tgtaccacct acagttacag
    39901 caacagcttc gtttttagtt acgccaagtt tgctaactat aggataagca accaaagtgt
    39961 gagtagcaaa catgcttgcg gtcaacacac ttgcattaat gccatagccc aaaacataat
    40021 gacacaaagc atagcctatt gcaaatggaa aaataaaagt aaacacacca aaggttaaac
    40081 ttttatattt attcatttta aattgagcca aatttagctc caaaccagca acaaacatta
    40141 tatatagcaa acctatggta gagaatagct ctatagcact attattggct ataatattaa
    40201 agccataagg accgataatt ataccagaaa ttattaatcc tattattccg ggaattttga
    40261 acctttgcaa taaaattggc gaaagtaaaa ttataaaaag aataacagaa aacactaaaa
    40321 taggattact caaaggaaga gtaaattctg ctaataactc gctaaaaaat tcttgcatta
    40381 taaaaaattg ctatttagtt tttgcaaaaa taaaaaaata aatcagttaa aagttaaaag
    40441 agtttttagt gttgagtttt tagtgtttag tctattgctc aaacggattt aactttacga
    40501 actttaaaac tttttaactt ttaactgatt tgattgtgtt gcccttacgg gcaaggcttt
    40561 tgagggtcta actaccgcag gctacgccta cggttaatca catattgccc tacgggcaaa
    40621 cctttgagaa tcttgacgtt gcgacaaaat taaatactgt tttaaaaatt attttggaat
    40681 tttttaaact ggatttattt gtatttgtcc accaattgct cttaaaattt tcataattgt
    40741 ttcaaatcta ggattagaac cttctgataa tgctttatac aaacttgatc tgcttaatcc
    40801 tgtttcttta gcaatttttg tcatgcccat tgattttgcg acatgtccaa ttgctacaat
    40861 tacgtctgag tcatcacctt ctgctaaaac agtattaaga tattctgcaa tcatctcatt
    40921 actgtctaaa taatctgcta tgtcaaattt tgaggtttcc attatattta ttctttaagt
    40981 tttttcaaaa tatttttagc tttatttatg tctttctgct gagttgattt ttctccacca
    41041 atgagtaaaa taataatttt cccatctgat tctttaaaat atattctata tcctttagca
    41101 tagtcaattt ttagttccct aattccatca ccaacatgtt tacaatctcc aaaatgttca
    41161 tcttcttcaa tcttttgaat gcgaaacaat attatggctt ttgcttttaa gtctcttaat
    41221 tttcttagcc acttatcgaa atcagatgtc ttataaatta tatacataca tttctccaaa
    41281 cgtctccatt cggatacaaa attacataat atttttattt aaaacaaata tttattgcaa
    41341 atccgtgcaa attacagata gatgttgcga caaaaataga cactgtttta aaaattattt
    41401 ttgattttaa ataaattggc attgttaatt aatatttata tcatgaaact aacaaaggtg
    41461 tcttagaagt gttcaaatgc acatcagtta aaagttaaaa gttaaaaacg atgcactgaa
    41521 cgtagttgaa gtgttaaaag aggctgacgc gtctatagca tgaacggatt taactttacg
    41581 aactttatga actttaaaaa cttttaacta ttttatcgct tcgatagaaa atagaaaccc
    41641 cttatgaata ggattaagct tatcttccca caatgcaggg ctaatgcttt ggaaagcttg
    41701 agtaaaatag acttcagaaa atccaagatt ttttatatat gcaaacaatt cttcttttga
    41761 gaaatagtct gcattatgct gatatgattc ttttttattt tcgtcaacga aatttaaaac
    41821 gtttttataa aaagtttcca catttcctgt atcacagtcg tttaggcttg ctcttaaatc
    41881 gggagatttt gcatacaaat tatccatagc tgctacggcg ttatggtaaa gaaaagtatt
    41941 ttccgaatta aaaactaatt cttctgagtt taccaaactt tttataacat attcgttttt
    42001 atcgttaaat gtggctttaa ctctgccttt atgatttttt ataaaattat ttaatttttc
    42061 ttttgactct ctacattcaa atggtgcttt tggagtaatt aaatgatttt tataaagctt
    42121 gtatgaataa tcataacaat gaacttttga gtgaaaaact ccaccttttt tcaaaatcct
    42181 atatgcttct ttgcataaat ttttagtagc atcaattgat atatgttcta ttacaaaaga
    42241 attaaaaaca acttcagcat gattatctgg tagaggtatt ggagctaaag actccaaatc
    42301 ataaaatata tctattcctt ctttactata cttgcttgat tctaagtcga tataagtcca
    42361 tatatcatat ttacttcttt gagaacctgc acctatatta taaaatctct tttcttttaa
    42421 actctcttca ctatataaac tatgaatgta ctgctctaag aaatttatct ccaataattt
    42481 ttcctttacg aaagtaattc ttgagaaaaa tatttcaaaa attttcctaa aaaatttgga
    42541 atgctttata agcacaatgc ttgctaattt tagtttattc attctattaa atctattttt
    42601 gagaattttt acaaaattaa aataaataat gcaaagctat aaataattag aaaataatat
    42661 ttttgtgttt aatttttaat agatttgtaa aaaaatgaaa actatgctta gtcaaagatt
    42721 taaaaacgat ggtaaaccta tttacaaact aaacgaaata caattacaag caaaagccga
    42781 tgtagaacga aaaatagaaa atgggcaata cgagttcgaa gaaataaatt gtgcagcctg
    42841 caaaagcaac gaatacgaac tgcttgccga aaaagataga tacggattaa aatattatac
    42901 tgtaatatgc aaaaattgcg ggcttgttta cgtttctcca agaatgacag aaaaagcata
    42961 tgcagaattt tataatgctg aatatagaaa attgtatgta ggcgaaaatg tggctagcga
    43021 aaagtttttt gcagaccaag tatttagagg aaaaagaata tttcaattca taaatacaaa
    43081 ttatgagata aaaaacaaaa aattagatgt tttagaagtt ggttgcggtg caggtgggat
    43141 actttctgtc ttcaaagaaa atggacacaa tataacaggc atagatttgg gcgaagaata
    43201 tttaaatttt ggaaaggaaa aatataattt aaatttattt aaatctaatt taaatgatat
    43261 ggataaaaat tataaagctg atattataat ttattcccat gtatttgagc atattttaga
    43321 tttagacaaa gaattagatg caataaaaag tcatttaaaa ccagaaggaa tattatatat
    43381 tgaagtccct ggtataaaat atgttcataa cacttacgaa atgaattttt taaaatattt
    43441 acaaaatgct catgtttatc acttttcgct aaactctcta actaggattt tacataataa
    43501 caattttgaa ttaatctatg gagacgaaca aattagagca atttttaaac taaacaaaaa
    43561 tttacaagac aagatttttg aaaacgaata ttctacaatt ttaaattatt tacacaagac
    43621 tgaaaagatg agattcttaa atgtatttcg tttcaaaaaa ttaaaatggc aaataaaaaa
    43681 aatattttta aaattatctt ttaaataaac tgtacttata aaaattttag ctatatttgt
    43741 aataaataaa ttaatactta ttattatgaa agcattgttt actttagcat tattatttat
    43801 tgcttgtctg tcttactctc aacaacaatt acttgattct gttgaatata tttattgggg
    43861 agactatact gatggcacaa ttagaaaagc taaattagat ggttcagata tagataccat
    43921 agtagatgat gattctaaaa taagaagaat taggcttgat agaagagata gaaaaattta
    43981 ttgggcaagc ggatattatg gtgctattcg ctctgcaaac tatgatggga gtgatatttc
    44041 tacagtttta caaacatctt tagatattga tttaattgaa gttgaccaaa aaaataaaaa
    44101 aatttatttc accgaagact atactggaac aattaaaaga tgtaatactg atggcacaga
    44161 gctagaaaca ataattactg gaactggctt ggtcttagga atggctattg acacagtaag
    44221 agatatactt ttctatggag attatactaa taaaaaaatt tttagagcaa aacctgatgg
    44281 taatgatatt accgaaattt tttccacaga caagcaaatt tttgatttat atattgatat
    44341 taaacaaatg tatgtttatt ttaacaatag aactactaat gcaattcaca gaatttttta
    44401 tgacggaagt ggttttacag atattattca atgttgtagc aataattgtg ttgtcggctc
    44461 tttggctgtt agtgtagaat ataactttct tttctggatt gaaagagaaa atggtatttt
    44521 agcaagaagt aatgccaatg gtgaagacag aacagatata atatatgttc ctggatctaa
    44581 tttttcagga atggacatag gcaaagaata tttaacccat gtaaaagtag atgaaaataa
    44641 gttgaaacaa attaaaattt atcccaatcc ttgttcaaac cagttttttg ttgacgcaga
    44701 aataaattct gatgtttcta tttataattt atatgggcaa aaaattaaaa cttcaaaact
    44761 aactagctct aaatcaatag atattgacga actacccaca ggaatttatt ttgtagaagt
    44821 aaaaaacgag aatgatgtgt ttaagcaaaa aattcaagtt gcaaaataat tttcattcca
    44881 ttacttattt gttctaatct cataaagcaa agattttaat ttatataaat ctttttctaa
    44941 atttgtgttt ttatatttaa agtttatgag aaatttagtt tttagttttc ttatcctttt
    45001 tatgttttca tcttgtttaa aaagggttga agttattgaa ccaagtcagt ttgagttaaa
    45061 ttataacgac aaacttgcct ttgcagaggc aataggaagt gatttttttg aaaaattaga
    45121 aaattacaaa tccgacagtt tagtgtatca agatttacaa tatctgttta cctacatgcc
    45181 acttagtgat tttgcggatt atgattttga gttcttttta aatcaagtaa aatatgcaca
    45241 tttaataaaa gaaacttttc cttgggcaaa agatattccg caagatattt ttagacatta
    45301 cgttttacct caccgagtta acaacgaaaa cctagacact gctcgcatgg ttttttatcg
    45361 tgaacttaaa gacagactgc ttcctttaaa tttaactgcc gaagaagctg ctttggaagt
    45421 aaaccattgg tgtcacgaaa aagtaattta taaacctact gatgcaagaa caatttctcc
    45481 tcttggtgct gttcgttctg gctttggtcg ctgtggcgaa gaaagtactt ttactgttac
    45541 agcattaaga gctgcgggca ttcctgcacg acaagtttac acgccacgct gggcacatac
    45601 agacgacaac cacgcttggg tagaagtttg gattgatgga gaatggaaat atcttggagc
    45661 ttgcgaacca ctacccagac taaatatggg ttggtttact gtgtctgcaa caagaacaat
    45721 gttagtgcac acaaaacagt ttggacaaga tgatgaagaa agttcaaact accttggaag
    45781 taatgcaaat tatacttggg taaatgctat gaaaacttat gctccaacaa aaaatttaat
    45841 aatacttgtt gtaaataaaa acaatatgcc tgtttggaat gctgaagtaa gatttcaatt
    45901 atataattat gcagaaatgt acccattgca tactgccaaa acaaatatta atggaatggc
    45961 aaaatttaca acaggatacg gtagtttaga agtttctgta agctacaaag acaaaacaat
    46021 tagtaaaaca ataaatccac aagatgaaaa cataattaca ctaaatattg atacagaccc
    46081 aaaatttcca gcagaaaatg ttgtgtatta tccaccagta gcaggcgaaa accacataat
    46141 cgatcctgag gaagaaaaac taaataacga aagactaaaa caagaagata aaattagagc
    46201 taacaacgaa agttcgtatt acaaccagtc taaaacaaaa gattttataa aagcatttga
    46261 ctatcccgaa agtgtagcag aaattcttat tggtagcaaa ggaaattgga tggaaataga
    46321 agctttttta atagaagcaa gctataaaaa ttgtaaaaat gaagctctag cacttcttac
    46381 tgaattatca gaaaaagatt ggagagatac aaaaaaggaa attttatcta aacattgcga
    46441 attggctatt aaacacagaa acagcaattt agctgatagt atattcaata agtatgtgct
    46501 taacccacga atacattttg aactattaac aaattacaga cctgccattt tagaaagttt
    46561 aaccgatgaa aacataaaaa actttaaatc aaatcctgaa aaaatatatc aatacatttc
    46621 agaaaatatt aaaacacaat ttaatattaa tggcaaaaat attgatgttc aagactttaa
    46681 taattacaac gtaagtatta cgccacttgg tgtaaataaa gttaaacatg ctgacccatt
    46741 gtcgcttaaa atttatactg ttgctttttg tcgaagctta ggtattcctg cacgaataaa
    46801 tttagcaagc aattttgcac aatattttca caataatgaa tgggtagatt ttaattttga
    46861 taaagccaaa gttgaaaaag ctaaattatt cttcacaaat cccgatacta caagagatct
    46921 aaaatataga gttcattttg caatagcaag attagaatca ggatttttca acactgttga
    46981 cttgggctgg gaaattccaa ttagcgattt ttctgacgga atagaagttt tggacggaaa
    47041 atacatgctt cttactgcat atagaaattc tgacgaaagt gttagtgtaa atagaaaata
    47101 ttttgatttg aaagcaaacg aaacaaaaac aatagaagtt acaatacctg aagataatag
    47161 tatttcacta aatattaaag ccttcgttca tggcaattta actgataatc aaacaaatag
    47221 cctcatcgac agtaaattaa tttgcaaaga aaatactgca actgcttttt gttggataaa
    47281 tcctacgcaa gaaccatcaa aacacataat tagagatatt aatttaggtt taaataactt
    47341 aaaaaacaac aaaataaatg ttgtttttat aactgatggt tctgaaattc aaggactttc
    47401 taaagaaatc ccaacttata ctgacaaaaa ttttgaattg ctaaataaaa actcagcttg
    47461 ctcacttagt gcaaatagca atgtatttcc aaatataatg ttagttagca ataataattg
    47521 catattttca agtaaggggt atataattaa tgttagtgag atattaatta atgaatggaa
    47581 aaaatcagtt aaaagcgatg caatgaacgg agttgaagtg ttaaaagtcg aaagtaatgc
    47641 gaattgatat aattactgtt ttgcccgaat tgattgctgg tccgttagat ttgtcaatta
    47701 ttaaaaaagc aaaagacaag ggcatagtag aaataaatat tctcgatttg cgagaatatg
    47761 gtttgggaaa atacaaacaa gttgacgact acccatatgg cggcgaagct ggaatggttt
    47821 taatgattga gcctatattt aatttgatag aaaaattaaa gtcagaaaga aaatatgatt
    47881 tgatttttta tacctcacct gacggaaaac cttggactca aaaagaagct aacaaaataa
    47941 gtttactcga aaacataatt attttgtgcg gtcattataa aggaatagac caaagaataa
    48001 gagaacactt aattgacgaa gaatatagca taggcgattt tgtactaaca ggtggtgaat
    48061 tggctgcagc aatgatgtgc gatagtattg ttagacttct tcctggtgct ataggcgatg
    48121 aacaatctgc gttaagcgat tcttttcaag ataatttgtt agctccacca gtttatacac
    48181 gccccgaagt ttttaataat tggaaagttc ccgaagtttt gctttctgga aattttaaaa
    48241 aaattgaaga atggaaagaa gaacaagctt atgaaagaac tttaaaactt agaccagact
    48301 tattaaaaaa tgatgagtaa aatttcacta ttttattatc tttgcacttt aaaatttagt
    48361 gatgagcagt cttttacaag gtgaaaattt aagcaaaaga tatggtgaaa aacttctgtt
    48421 tgaaggaata aatattaata tttcagaagg agacaaaatt gcattaatag ccaaaaacgg
    48481 aacgggaaaa acttctttac taaacatttt aacaaatact gatacgccag atagcggaaa
    48541 tattacaagc aaaagagatt tgcgtttagc atacctgcct caagaactaa atttagaccc
    48601 cgaaaaaact attttagaag cagtattttt cttagatgac gaaattatta gcacaatccg
    48661 agaatatcat tctgttttat cttcaaataa tcatgctgac ttacaagcaa tattagacaa
    48721 aatggatagc ttaaatgctt gggattacga aactaaagta aaacaagtat taagcaaatt
    48781 aaaaattgac gatttttcaa aaaaaataaa atatctaagc ggaggacaaa aaaaaagagt
    48841 agcacttgcc ggaactttaa ttagcgaacc agatttatta attttagacg aacccacaaa
    48901 ccaccttgat ttagatatga ttgaatggct cgaagaatat ttaacaaaaa ctaactccgc
    48961 aatttttatg gttactcacg atagatattt tttagataga gtttgtagca ccatttttga
    49021 gttagatatg ggtcaaattt ttatttacaa tgggaattac tcatattttg ttgaaaaaag
    49081 agccgaaaga atagaaaacc tacaagccaa tattgacaaa gctcaaaact taatgcgtaa
    49141 ggagcaagaa tggataagac gaatgccaaa agcaagaact tcaaaatcaa aatatagaat
    49201 agaagcattt gatgaactta aagaacaagc atcacaacga attgaagacg aaaaattgca
    49261 atttggtata gctaaccaaa gattaggcaa aaaagtaata aacatttata atatttctaa
    49321 atcctttggt tcagaaattt taatatcaga cttttcttat aaaattgcta aaaacgaaaa
    49381 aattggaatt attggaaata acggaagtgg taagacaact tttttaaata ttttaaccaa
    49441 caaaataaag cctgattcgg gcagggttga aattggagaa actgtaaaaa ttgcttatta
    49501 cacacaagaa ggtttaagct ttgacgaaga ctctaaacca attgatataa taaaaaatat
    49561 tgcagaaaca gtaaaattaa aaaatgggaa aacactttct gcttcgggat ttttgcaata
    49621 ttttttattt tcgcccgaaa tgcaatatac ctatgtaaaa aaacttagcg gtggcgaaaa
    49681 acgcagatta tatctatgct caattttaat gactcaaccc aacgttttat taatggacga
    49741 acccacaaac gaccttgata ttatgacttt gcaggtttta gaagaatatc tatcagaaat
    49801 tgaagcaagt gtaattattg tttctcacga tagatttttt atggataaaa ttgttgacca
    49861 tgtttttgtt tttgaaggaa atggtaaaat tttggacttc cctggcaatt acacacaata
    49921 cagatattcg ccttattatc agagacttaa ccaagagcaa aaaccagaga aaaaagaaag
    49981 tccaagtaaa gaatctttgc caaaaaatgt ttcgcaagat aaacccaaaa aagttagttt
    50041 taaagataaa tatgaatacg aacaactaaa caaagagctt gaagaattaa accataaaaa
    50101 acaaaaatta gaaatagaaa taaattctgg taatttcaat catgttgagt taaccgaaaa
    50161 atcggaagaa tattcaaaat tattagaaat aatcgaagaa aaagagttta gatggttaga
    50221 attagatgaa cttataaatc aataaattaa tacaacaaat aattttttaa aaatttgatt
    50281 aagtctttta ttattttatt tatcttgcaa aaaaattaca attatgttaa atattgcttt
    50341 atttggtcct cctggagctg gaaaaggaac tcaatcaaaa attttaattg aagaatataa
    50401 tttatcctac atttctactg gtgaattatt acgtaaagag attaaaaata aaacaaaatt
    50461 aggcttagaa gctcaaagta ttattgctgc tggtggtttg gtttctgatg aaattattgt
    50521 tcaaattatt gaaaaaacaa ttacagaaaa tcccaatgca aatggttttt tgtttgatgg
    50581 ttttcctcgt acatatattc aggcttatat actcgaaggt ttgatgatta aattaaacac
    50641 ctcgttaaat tgtttgataa gcttagcagt tccagaagaa gtttctgttc agcgtttatt
    50701 agaacgtggt agaacttcgg gcagaacaga cgataacgaa acagttatta gaaatcgatt
    50761 aaacgaatat tacgaaaaaa caatacctgt aattaatttt tacaaagaaa aaggaatttt
    50821 ttcagaagtt gacggaactc aatctgttga gaatgttaga aaagacataa aagctattgt
    50881 taaaaaagag ttgagcaaac gtttgttaaa tattgtttta tttggttatc ctggttctgg
    50941 aagaggttcg caaggcagag ctattgccaa aaaatacggc ttagaatatg ttgctacagg
    51001 acctatgcta cacgaagaaa ttgaaaaagg tagcgaaata ggaaaacaaa ttaaagattt
    51061 gtttgacaat ggaaaactaa ttcccgatga gattgtggtt cctttgattg aaaagaaaat
    51121 tgaaaattca aaaggtgtaa aaggttttat ttttaaaggt tttcctcgaa ctcttgtgca
    51181 atcctatatt ttagatggct tattgaaaaa gcactcttcg tcaatttcta aaattattga
    51241 aattgaagtg cctactcttg aattaattaa aagattagac gaaagaagta aaactgaaaa
    51301 tcgcatgcct tatgactcaa gtactgctaa aattgttcaa agactacaag accaccaaac
    51361 caaaactgtt cctgttatag aaaaatacaa cgaattgcac ggagttgtaa aaattgacgg
    51421 aatgggcagt ttcgacgaag ttttcaaaag aataagtttt gaaatcgaaa atggattcga
    51481 gaatctgaaa taaaaaaaga caacttctgc aatgcaaaag ttgtcttaac caattaacat
    51541 aaaaacaata tttatctttc aactatgaaa gctacttttt taagtttctg attttctcgg
    51601 ttagaagtgg tacaattttt tcaacctcgc caacaatacc aatatctgca actccaaaaa
    51661 ttggtgcaaa cttatctttg tttatagcga taatgtactc actttcttcc atacctgcaa
    51721 ggtgctgaat agctcctgaa ataccacaag ccacgtaaag ttgtggacga acagttttac
    51781 cagtttgtcc tacttgtaaa tcgtgatcca tatttcctgc atctactgta gcacgtgaag
    51841 cagaaacttc gccgtttagc tcatcagcca attctctaag atgaccataa tattctttgt
    51901 gtacaccacg accagcagaa acaagcactg gagcttcagt aatgtctttt gttgtttttg
    51961 gttttacaac actttctaaa agtttcacac ggttaatttt ctccatattg aaacttacct
    52021 caactttctc aacttcgcct tttcttgctg aagtatcttc taagcgttgc ataacacctg
    52081 gacgaactgt tgccatttgt ggacggtggt ctttacaaag aattgttgcc ataagattac
    52141 caccgaaagc aggacgagtc attaagaact caccattttc gcctatttca agtttggtgc
    52201 aatctgctgt taaacctgta gtaacacgag ccgaaactct tggaccaagg tcacgaccta
    52261 ttgttgtagc acctaaaagt aaaatatctg gttttctttc gtttataatt tgacttagag
    52321 cctgagtata aggttcggtc atatagtttt ttaaagcatc gttatctacc actaaaacct
    52381 tgtctgcacc tgcctgaatc aagtcttgag ccttattttc tatatcttta cctaaaagca
    52441 cggctaccac tttttcattt aaagcatcag ccaactcgcg agccttacct agaagctcga
    52501 acgctacttt ttgcactacg ccaccgcgtt gttctgcaaa aacataaaca tctttatatt
    52561 gagtaatatc catattttct ttctttttta gggttaaata atgtgttgtt ctttaagttt
    52621 tgaaacaatc agatttacag cttcctctgc tgaaccttcg aatagtgaac ctgctggttt
    52681 aatactctta gggaatgctt ttgctacact tgtaggcgaa cctgcttttc caattttttc
    52741 ttcgggaacg ttgattttgt ctgctcccca aacttctatt tcttttttat aagctgcaac
    52801 tatacctctc atgtgcatat aacgtggttt tacagcagaa gccaaaactg ttacaagaca
    52861 aggagtatcc atttgtaaaa cttgatagcc ctcttcggtt tgttttctaa tagttaaagt
    52921 ttttttgcca tcaaaatcta agtgttcgag atacgaaact tgtggaatgt ctaagtgttc
    52981 agcaatttgt ggtcctactt gagcggtgtc tccgtctata gcttgacgac ctgtaataat
    53041 aagatcataa tccaaagtac gtaatgcgcc tgccaaagag ttggaagtgg ctaaagtatc
    53101 tgcaccaccg agtttgcggt cggttagtaa tatagctcta tctacaccca ttgccaaagc
    53161 ttcacgaaga atagcctcag cttgaggtaa gcccattgta ataacggtaa cgtgagcatt
    53221 gtatttatct tttagttgca gagcgaactc caatccacct ttatcgtcgg ggttcataat
    53281 acttggaact ccgtctctca taagcgtgcc tgtctgtgga ttaatcttta cttctgtagt
    53341 atcaggcacc tgttttatac aaactattat attcatattc tcttgatttt ctattttaaa
    53401 tttttaataa cttctatttt ttactaaaca acttagctgc aattaccatt ctttgaacct
    53461 ctgaagttcc ttcgtaaatt tcggtaattt tagcatcacg catcattctt tctacaggat
    53521 aatcacgagt atagccatat cctccatgga attgaacagc cttagtggta acctccatag
    53581 cagtttctga agcaaataat tttgccattg ctgcatcaac agagtaagga ataccgttat
    53641 cctttttata atgagcattg cgaactaaca aacgagcagc ttgaacctta gtttccatat
    53701 ctgccatttg aaactgtgta ttttggaatt gcgatatact acgaccaaat tgcttacgct
    53761 ctttggtata ttttatagtt tcgtccattg ctccttgtgc aatacctaag gcttgtgcag
    53821 cgatacctat acgtccgccg tcaagagttt tcatagcaat accgaaacct cttccaacct
    53881 gtccaagaag attttctttt ggtatgcgac aatctgtaaa tacaagttcg gcagtagcag
    53941 aaccacgaat accaagcttt ttctctttct taccaatctc gaagcctttt gttccttttt
    54001 ctactataaa agcagaaatc cctttattac caagcgactt gtcggtcata gcaaagataa
    54061 tgtaaacgtg tgcatattca gcatttgtga taaaaatctt agttccgttt aacacatact
    54121 catctccatc aagcacagcc attgtttgtt gcatagctgc atctgtacca gcatttggct
    54181 ctgtaagacc gaaagctcca atccattcgc ctgaagcgag tttaggtaaa tactttttct
    54241 tttgttcctc agtaccgttt tcccaaatag gtgctacaca tagcgactga tgtgccgaaa
    54301 gtactactcc tgtagtacca catacgcgtg aaatttcttc cacagctata ccgtacatca
    54361 tgtttgtgcc accagctcca ccgtattcca caggtacagg aatacccatc aaaccaattt
    54421 ttgccatctt ctctactgtt tcgattggaa atctctcctg ttcatcaatt tcggcagcca
    54481 aaggttttat ctctttttct gcaaactctc ttatcatttg cagaaaaagt tcttcggttt
    54541 ttgataaact aaaatccata attatactac tttttatctt ttcttttaat taattttatc
    54601 aaaaataaaa ttcagataat ttttcctatc tgaaaaataa attgcaaata tataaattat
    54661 ttaccataat gaaaagatat atcgtcaaaa ttaaaatttg tttgaatagc atatttgata
    54721 aaattaaagc catctcttaa tttaacataa aaattactta taaaaagtta ctttattttt
    54781 attatgcttt tcaatttctt cttcaacagt tgttgtaaat ccaaattctt ttctacgttc
    54841 attcactttt cttttttgct tagttggagc taactcgcaa ggttgagtaa ttattaaacc
    54901 tctatatttc tgatcaagta ttccatgttc cataatttta attgctaatg ctgtgtctgt
    54961 aataggacca ctttcataaa gaaatttttc aataagcaat tgataaacat tttcccaata
    55021 attactttct cgcaaagcac ttattctata atctaaaaaa atagcgggag ttaagttata
    55081 ttcaaccttt tttataaaat ccattagatt tttttgagct ttttcaaaat ctcccaaatt
    55141 tgaataacat attgccaaat tgtaaatatc tgagttagac aaactatctt tagcgaaaat
    55201 atcatctaag gcttcttgaa acaagttttg ctcaacataa tcatttaata ctggatagca
    55261 atacatcatt cttttttgag aaaatgaaat atttaggcta aacacaaata atattgttat
    55321 tagtaaatat tttttcatat tctgctattt ttatttctac aaagatattt ataatttgaa
    55381 aatagaattg aaatcttgtt cagtaatttt ggatttgctt aatattgtgg gacatattct
    55441 gcagagaaaa tatccagaag cttttgaggg aaataagatt atataaaact ctgactcata
    55501 gttttctgct tctataattt gttttatttt tcccttccaa cctgattgaa atattggaaa
    55561 ataaaatgca ctaactccaa atttcgaaca aatattatta attctaaata attctcgaaa
    55621 agcgtttttt ttatgagttg agtaatattt ttttgtaggc aagattaaaa ctttatcata
    55681 ttgtgcacat tctgctattc cgagtatttc aaacattgaa ttttcgatat caattatacc
    55741 tgtaagctca tctacataaa tataatctag cggtgataaa gaaacataaa atcttttatt
    55801 ctcatcaaga ataagtctct gttcaggaaa tttccaatat tgatacatca gaatttcata
    55861 aacctgattc caagaacttt cccaattttt agcagattcg tatgccttgt ctaaaaaaaa
    55921 caggaaattt tctcgtccaa aatgacttat aaatacagat acagaagaac tttcataatc
    55981 atagtctatc atagaaatat ctcgttgcga caaaaacttg ggccaaccat tattgtttac
    56041 ccaatctttt agtttgctac aatttataga gtcttgcttt gcaacaaggt cctatgaaat
    56101 tttcttttta ttatatttta ctctattatt ttggtcagat tccgatatta aatccaataa
    56161 atcaattagc tctttatata ataagtactt tcataaaatt ttctattttt ttcatatctt
    56221 agcgaatcgt attctaattt tatatcagaa aaaactaagc caaatcttac agctttgtca
    56281 agatagttaa gacttttgtt tctatgttta tgtttacatt caaaatattt acccaaattt
    56341 gatgtagttg gtaaaactaa taaatttgcg caagctttta ctttgttatt gtgcaataca
    56401 gcaagtctat aataggtttc tggcaaaaat gttttgtctt ttgaatttat aattttcgaa
    56461 gcataaaaat aagtgctatc aacatttcct ttattagaaa aatcaatcag atgcctaaag
    56521 ttaaattcat tgtgaacata ttctttcaaa ctttcttgag aatatgtaag aattggaaat
    56581 actaataata aaaataatag tttttgtttc atttgttatg tgtttttttg caattattaa
    56641 gcaagcaagc ctaccaaaac cttctaaacc caattatttc tttaacatct tttaaagttt
    56701 tacttgcatt ttcgcgtgct ttttcagctc ccatttttgc tactttactc aaatattcgg
    56761 tgttagaatc aatttcttta attttttgat ttataggagc taacattttt attacatctt
    56821 ctgctatttg ttttttcaaa tctccataac gaatttcaca gtttgcgtat tgttcttcaa
    56881 aaaatttaac tgtatcagga gtagaaacta ctttcattat agtaaaaaga ttttgaatag
    56941 attctggcat ttcagaattt ttctcagtag gtccgctatc tgtaagtgct ctcattactt
    57001 tttttcgtat aacagatgct tcatcggcta aataaattcc attgccttcg gatttaccca
    57061 ttttaccgct accatctaag ccgggaattt taattaattc ttttccaaaa ttaaatgctt
    57121 gaggttcttt aaaatattca acagaataca tattattaaa tcttcgagca aaccttcttg
    57181 ccatttcaag attttgttct tggtcttttc ctacaggaac tttatcagca ttatgaataa
    57241 ttatatcagc agccatcaaa acaggataag tcaataaacc tgcatttaca ttttcgggtt
    57301 gctgacgagc tttttcttta aatgttgtag tgcgtgctaa ttcgcccata tatgcattca
    57361 tatttaataa cagatacaac tctgttattt ctggaacatc agactgaata tataaagtgc
    57421 ttttttctgg gtctattcct gctgccaaat attcaaccaa aacttgccta acactattat
    57481 gtagattttc aggagttggg tgagttgtta acgaatgata atctgctata aaaaagtaac
    57541 aattataatc attctgcatt tttacaaaat tctgaactgc acctagataa tttcctaaat
    57601 gcaaattccc tgttgaacgt attccactta aaaccgtttc cataattata aatttgttgc
    57661 aaacttaaac aaaaaaaata attttacata acgctacaaa aacaaaattc ttgttaataa
    57721 aaaaattcat tttatttgaa tattgttttt atatttgtca agaattttta aaaaattata
    57781 aacctataag caaaatctta taatatgcaa aatcttaaca aaccaaacta catttttgaa
    57841 gttagctggg aagtatgtaa taaagttggc ggtattcata ccgtaatttc aactaaagct
    57901 cttgaattag caaaaaattg tgaccaccac attcttattg gtcctagcat ttggagagaa
    57961 acaaaagagc atcccgaatt tattgaagac aactccttat ttgcggattg gaaacatcat
    58021 ttagagttaa aaaacacaca tattcatatt ggctattgga atattccagg aaaaccaaag
    58081 gtgtttttaa ttgatttttc aaaatatatt agcgaaaaag acagcattct tgcaaaattt
    58141 tgggaagact atagattaga ctccttagcc ggacagtggg attatattga gcctgcttta
    58201 tttggatatg catctgccaa aataatagaa agttttgtaa acttttatct ctctgtacac
    58261 gacacaatag tcgcacattt ccacgaatgg atgactggta caggaatttt atatttaaaa
    58321 tcgcaaatgc cacaaatagc aactgctttt actactcacg caactgcaat aggacgtagt
    58381 attgctggca acggattacc actttacaaa aatctttcta attataacgg cgacatctta
    58441 gctggtgagt ttaatatagt ttctaaacaa agtctcgaaa aactaagtgc tcacaacgct
    58501 gatgtgttta caaccgttag cgaaattacc gctcaggaat gtagccaact tttaagcaaa
    58561 gatgttgatt ttgttacgcc aaatggtttc gaaaataatt ttgtgcctca accagcagaa
    58621 tatagcgaaa aaagaaaaac tgctcgcaaa aaaattattg aagtagccca agctttaatg
    58681 aattataaat atgaaaaaga gcctgtaatt gtagcaactt caggacgtta cgaattttca
    58741 aacaaaggtt atgatttatt tataaatgct ttagcagaat taaataaaaa cacaaattta
    58801 aaagaagaag ttttagcact tattcttgtt cccgcaaaca actacggagc tgtaaatcag
    58861 ctatataata ttttaaacaa tgttgagggc gacaccaata ttgtaaataa ttttttaact
    58921 cataatattc acgatattga atttgaccct atttcaaaaa gaatttgcga aaaacaatta
    58981 ttcaacaaag ctgaaaataa agtaaaagta atttttgtac ctacatattt gaatggagat
    59041 gacggaatat ttgatattaa atattacgat ttattaccag gcttagattt aactgctttt
    59101 gtttcttatt atgaaccttg gggttacact ccattagaat ctgtagcttt tggaattcct
    59161 acaattacaa caagtttagc aggatttgga aagtggatgc aaggagtttt agataaaaat
    59221 gataaaagtg ttaaggttat taacagaacc gatgacaatg cagatgaagt tgttgctgaa
    59281 ataattaatt attttaattt ttatttaagc ttaaatcaaa aagaaagaga aaatttatct
    59341 aaatctgcat ttgctgcttc tgacaatgct ttgtggacta acttaattaa agaatatgaa
    59401 aaagcatata gctttgcttt agaaaaagtt aatgaccgtc aagacgaatt tgttaaacaa
    59461 attccaagca gaccaataag cgaaacctat gataaggaat cacactctcc tcagtggaga
    59521 aaacttgagg taaaaactca tgtaaccgac agatttaatg ctcttattgc tatttcttta
    59581 aatttatggt ggacatggaa caaaccggct agaactatgt tcaaatatat agatcctgaa
    59641 ctatggatag aacatgcgaa aaatcctgtt acattcttag aaaacgtctc tatttcaaga
    59701 ttacaagaat tagaaaacga taaatatttc actaatttat acgattcggt ttgcaaagaa
    59761 ttttacgaat atattgctaa gaaagaagag aaaaaagctc caaaaatagc atactttagc
    59821 atggaatatg gatttgacga taatttaaaa atattttctg gaggtttagg aattttagct
    59881 ggcgactttt taaaagaagc tagcgataca aatactgatt tagttggaat tggactgctt
    59941 tacagatatg gatattttaa gcaaaaaatt acttccattg gagaacaaaa tgcagaatat
    60001 atcccacaaa attttgacaa aatgccaatc caacctgttc gtgatgataa aaacgagcaa
    60061 atgaaaatta tggtttattt ccctggtaga aatgtatatg caaaaatttg gaaagccaat
    60121 ataggaagaa ttccgctata ccttttagat actgatgtgg aagaaaatca agaacaagac
    60181 aaatatatta catctcgttt atacggtggc gatatagaat ttagatttaa acaagaaatg
    60241 atattgggag ttggaggtat tagagcttta caggctttaa atatataccc tgacgtatat
    60301 cattgcaacg aaggacacgc agctttcata ggattagaaa gacttagaat tttaagaaca
    60361 agacgcaatt taaaatttga agaagcatta gaaataataa gagcttctac cctgtttaca
    60421 actcacacgc ctgtccctgc tggtcacgac actttcgatg aaaatttaat gagaacttat
    60481 atgtctcact atccagaaag actaaaaatt acttgggatg aaatgatgag acttggaatg
    60541 ttaaataaag gcgataaatt ctcaatgtcg tatttagctg ctaatgtgtc tcaagaaata
    60601 aatggcgttt ctatgcttca tggacaagtt tcaaaagaaa tgtttaaaga tttatggaaa
    60661 ggttatttcg cagaagaaaa tcatgttgga tatgttacaa atggcgttca ttaccacaca
    60721 tggacagctt cggcatggca aaatttatat ttaacaactt ttggaaaaga atttttaaac
    60781 gacttatcta atcaaaatta ttggagtaaa atccaagatg ttgatgatga aataatttgg
    60841 gatattcgcc aaaaacaaag agcaaaactt gttaattttg ttaaaaataa agttagacga
    60901 aattggataa gacgctatga agaccccaaa aacttagttg cagttacaga aaaaattaat
    60961 gaaaatgtac taacaattgg ttttgctcgt agatttgcaa cctacaaaag aggagattta
    61021 cttttaaaaa atcctgaaag attagcaaga attttaaata atcccgaaaa acctgttcaa
    61081 attttatttg cagggaaagc acacccaaac gacaaagctg gtcaagattt aattaaaaag
    61141 attgttcaaa tatcaaaaca accagaattt ttaggcaaaa taatttttgt tgaagattat
    61201 gacattagct tagcaaaaca tttagtacaa ggagttgata tttggctaaa cacacctact
    61261 cgtcctcaag aagcaagcgg aacaagtgga atgaaagctg ttatgaacgg agcgttacac
    61321 ttctctgtac tcgatggctg gtgggtagaa ggatataaag aaggtgccgg ttgggcttta
    61381 cctgaaaaaa gaacctatca agaccaagag ttgcaaaatg aattagacac acaaacaatt
    61441 tattctctac tcgaaaacga aattgttcca ttattttata atagagacga aaaaggcatt
    61501 cctcacgatt ggattaaatt tattaaaaat tctatcgcta caattgctcc acagttcaca
    61561 accaaacgaa tgatagatga ctattttgat aaatattaca acaagctata tcaacgtagc
    61621 gagttgatga aaccaaacct ttacgaaatg gcatctaaaa ttgcagattg gaaaaaaagc
    61681 gtaaaaagag gttggaatga tttagaaatt gtaagtgtga aatttcctga ttttgataaa
    61741 catcctctaa gcgttaacga aaactttact ggcgaaattg aaataaattt aaaaggtctt
    61801 agctcagacg atattggagt tgaggttatt gttaccgatg ctacaaccaa tggctttaca
    61861 aaaatttatg ctattcatca agcagaatta gtagaagtga aaaataaaat tgcaagatat
    61921 attgtcaatt cagcacccaa aaaacctgga ttttacaatt atgcaattag agtttatgct
    61981 aaaaacgatt tattgccata taaacaagat tctggcttag tgctttgggc ataagtttta
    62041 acacttctga aacaccttga aaacttgctg gtttaacaaa tccgagcgag cgtcatgggt
    62101 gttgaagcga gcagagtgag caaagcgaag tctgcgaagc aaacccaagc gagcaaagcg
    62161 agcaaataat tcttaattat cgggtgttga gcgaagccga acgggtgttg agcgaagtcg
    62221 aacgggtgtt gagcgaagtc gaaacacaat tcttaattct taattcatct tcatactcaa
    62281 cactaaaaac tcttttaact ttcaactgtt ttttttgtat tgcaataaag ttttaccact
    62341 tccttagctt ctttaacatc gtgaactctt aaaatatgag agcctttcat tagagctatt
    62401 gtatttaaaa ctgtagtgcc atttaatgct tctaaaggag taattcctaa agttttccaa
    62461 atcattgttt ttcgtgaaag tccagctaaa ataggaagtt caaacacttt aaatctttct
    62521 aaattattta aaagttcaaa attttgtgct acggtttttg caaatccaaa tcctgggtca
    62581 acaattatat cattaacgcc catagaattt agttgtgcaa ttttttctga aaagtaaaaa
    62641 ataatatctt cgataaaatt ttcataatca gttttttctt gcatgttttc tggtgtgccg
    62701 cgcatgtgca taagaatata tggaacatct aagtcggcaa cagtttcaaa cattttttta
    62761 tcaaattcgc caccgctaat atcgtttatt atgtctattc caaaatcttt tactgctcgt
    62821 tttgaagttt cggcatacac tgtgtcaagc gaaaaaataa tattagaata ttttttatta
    62881 actaaattta aaactggttc caaaagttgc cattcatctt cttgttttaa aatagttaaa
    62941 cctggacgag tagaaactgc tccaatatca atcatatcag caccatctgc gagcaatttt
    63001 tcaatacgtt tgtcggcatc ttcaacttga ggaaaaatac ttccactata aaaagaatct
    63061 ttactaagat ttaaaatccc catcactaaa ggcgtatcaa cacttaacaa ttcagatttt
    63121 acttttataa acataatatc taattttaaa atttaagcaa aaatacattt tttaaaactt
    63181 atagaatatg atttatcaaa acctattcta tgcttttaag cacttcgcaa attattaatg
    63241 aattattaat aaaatattct ccaaaagcta tgagggtttt agaaaatttt gtatttttgt
    63301 gattgttcgt taaaaaaata tataatggct ttttttgtta ttgaaggttt agatggtgct
    63361 ggaaagtcaa ctcagttaga gcttttaaaa gaccattttg caaatattgg taaaaaaacc
    63421 gaatatatac attttcctac aaccgactct cctatttttg gagatttagt gtctcgattt
    63481 ttacgcggag aatttggcgg aattaaagat gtaaacccat atttagtagc tttattattt
    63541 gctggcgaca gatacaatct taaaaacaaa atcaactctt ggttagagga aggaaaaatt
    63601 gttattaatg atagatatgt tttttctaat attggttttc aatgtgctaa aatgaataac
    63661 gaaaaagaag ccgacgaact ttttgattgg atatttaact tagagtttga gtattttaaa
    63721 attccaaaac ctgatttaag tattttctta gatgtaccgt tttcatttac cgaaaacaag
    63781 ctaaaacaaa ctcgcgaagg gaaagacaga gaatatttac aaggaaaaaa agatattcac
    63841 gaagaagatt taagttttca aaaatctgtt agaaaaactt atttgcgagc aatagaaaaa
    63901 tatccaaact taataataag aatagattgc tctgatgaga atggacatat tttaagtcct
    63961 gagagtattt caaaattgat aataaaagaa attgaaaaac gttttacttt ttaaaaataa
    64021 ttattatgaa attcttgaga aattttagtc gtttatttac aggtataatc tttatttttt
    64081 ctggatttgt aaaagtaata gaccctcttg gttctgcata caaatttaca gattattttg
    64141 ttgcaatgca ccttgacttc ttaaacgaag gagcattagt ttttgcaatt ttaatgtcta
    64201 ttgctgaatt aataattggt attgcacttg tttttaattt attaccaaaa attgctgctt
    64261 ggttactact gctttttatg gcgtttttta ctccattaac actttggtta gccgttgccg
    64321 acccagttag cgactgcggt tgctttggag atgcaattat tttaaccaat tggcaaactt
    64381 tttataaaaa tttagtaatt ctggcattta caataattgt attctggcaa agaaagttat
    64441 ttaaacccgc gtataactta ttcaaccaat gggcattaac aatagctttc acaattgctt
    64501 cgtttgtact aacattatac tgcttataca atttaccaat agttgatttt agaccttatc
    64561 acattggagc taatattcaa gaaggaatgc aaattccaga agaagaaaaa gaaaatgttg
    64621 acatttacga aagtgttttt atttacgaga aaaatggtga acaaaaagaa tatagcgaaa
    64681 ctgaattacc agattcaaca tggacttttg taaacgccga ccataaacta gtgaaaaaag
    64741 gttacgaacc tcctattcac gattttacta ttgaaccaat ttttgttcct ggctatagcc
    64801 ctgaaccaga aaataaatat gtaaatcctt gggatttaga atttgagttt acaaaagatg
    64861 gcgaaactat tacttgcgat ttagatagtt taccagacca atcttggaat tttaaaaaaa
    64921 taatttacaa cacaaaactt aatcctgaca atttaaaact atatttctta aatgaagaag
    64981 gtgaagaaat tattgccaac ataaaagatt tgccagacaa caactctata ttccttgatg
    65041 cagaatatat tgatacagaa aacgaaaact ttttattaaa atatggcgaa gacattacca
    65101 atcaagtact tgaagatgaa tcatatgcgt tttttgctat tatgacatta cttgacgagg
    65161 ttaatgaaaa acatttagat aaagttgcac aaatttcaga attttgtaaa aacaacaatt
    65221 acaaatttta ttgcataaca gcttcaaact tagaagaagt ttcagcattt ataaattatc
    65281 acaaaccaaa ttaccaattt tacaatatgg atccaataac cctaaaaact attgtaagat
    65341 caaatcctgg attagtttta gtgaaaaaag gaactgtact aaacaaatgg gcggcaaaaa
    65401 acattcctgc tcctgaacaa ttacacaacg acctaactgc aaattcaata acaaaacatc
    65461 aaaaagctaa aaacaagtat atttatctaa cttatatttt tggtactttg ttatttatga
    65521 gtttatttca tggattttat aagtacttaa aaacaaatag atatatataa actaaaaaat
    65581 ataattttat gaggaaaaaa attgtagcag gaaattggaa aatgaatact ttgccagcag
    65641 aaggtgctga attagcaaag accatttgtg aacatgttaa aactttaagt caagaagaaa
    65701 aatataaaat aattcttgga gttccattca cacatattga tagagttgtt aatgctattg
    65761 atttaaaatt tgtaaaggtt tcggctcaag attgtgctat gtatgattca ggagcttata
    65821 ctggtgaagt gtctgcaaaa atgataaaat caattaattg cgaatatgca attatcggac
    65881 atagcgaacg ccgtcaatat tttggagata ctgatgaagt gattgctaaa aagttagaaa
    65941 tgtgctatac cagcgattta tatcctattt tatgttgtgg cgaaaaatta gaagaaagag
    66001 aaaacaattc acatttcgaa gttgtagaaa agcaaattct aaatgcttta tcaaatgttg
    66061 atgctgaaaa aatgcaaaaa acaattattg cttacgaacc agtttgggca ataggtacag
    66121 gcaaaactgc cagtccagaa caagctcaag aaatgcacga atttattcgt aaaatacttg
    66181 caaaaaaata cggacaagaa attgcaaacg acacatctat actttatgga ggtagtgtaa
    66241 attctaaaaa tgcacaacaa cttttcgact gcaaagatat tgacggtggt ttagttggtg
    66301 gtgcttcgtt aaaaccagat gaatttattg caataataaa tgcaattaga aaatgaatta
    66361 tattgaagta aaggtaaatt ttaataaaga acaaaccgaa cttagcgaaa ttttaattgc
    66421 atttttagct gaacaaggtt ttgaaagttt ttatgaagaa aatgattcct tatttgcata
    66481 tattgaggaa gctaattata atctaaaaaa tctagacgaa ttactcaaat cttacaaagt
    66541 ttcttattca acaaaaatta ttccgcaaca aaactggaat gctgaatggg aaaaaaactt
    66601 tgaacccaca gtagtagaag atttgcttag tattaaagca ccatttcacg acatggattt
    66661 taatacaaaa tacaatatta ttatcgaacc caaaatgtcg tttggtacag gacatcatgc
    66721 cacaacttgg atgatgtgta aacttattgg cgaatacgat tttgaaaata aagatgtgtt
    66781 agattatggt tgcggaacag gtattttagc aatatttgca gctcttttaa aagcaaaaga
    66841 tatttatgcc atagatattg acgaatgggc ttatgaaaat acaatagaaa atgcaaatag
    66901 aaataatact ccgcaaataa aagctataca aggcgatata aattcaattc ctgataaaac
    66961 ttttgatttt atatttgcaa atataaatct caatgtttta aaaaaagata taccaacttt
    67021 ggcaaaacat ttgaacaatg gtggaaaaat cttttttagt ggatttttaa acaacgaatt
    67081 agtacaaata caaaaagttt gctcagaaaa taatttgtct tttaataaag caatacaaaa
    67141 agacaattgg actgcatgtg tttttaataa aatttaatta tgaaaaaatt agttttcttt
    67201 ttcgctttaa tattaatttc aaatttaata tttgcccaag cttttccaac tgaaccagat
    67261 aggtttataa aaagctttaa ttcagaactt ggatatactg gtgaagttgc tagcacttca
    67321 aaaaatttga gcaaagaatt tactactttt tgggaaaccg atagtttaag ttttcaagaa
    67381 aaagaaaaat ttattagcac tgcaaacaat atggctgcaa agggttgcaa aggctatccc
    67441 gattttgtat gcttagccga caatagaatg tggtttagta gaaaagcttt tgataattca
    67501 caatacgaaa tatatgaaaa aggaattttc gatattttaa atgcaggcaa aagaccaaaa
    67561 cttaatgatt tatctgatta tcttttaagt ttcaattatt tattatcaaa ggatattatt
    67621 gcaaaaaacc cacgtacaca ttggaaagtt gaaaataatt cttttgaatt aaaatatgat
    67681 aaaggaataa aaattcaact atctaaagtt aatctcattg gttatcaagg agttgatagt
    67741 ttaaaaattt atagaacaga tgccgaatat tacccttcac aaaacttatg gaaaggagtt
    67801 ggcggaactg ttggctggga acgtgttggc tatagtttag atagtattca agctcaactt
    67861 tctaattatc aaattaatac aaaaaacgta agtctaactg ctgactccgt aacttttaac
    67921 aacgtcatgt atattaaaaa acctatgctt ggcagattaa ttgacaaaac tggaaattta
    67981 gataatccta aaaaatcaga ttatccaaaa tttacttcat acaatcaaca ttattatttg
    68041 aaaaattttg ttcccggaat agattacgaa ggaggttttt ctgttcatgg aagtagtttt
    68101 atagcatctg gaacaaaaga agaaccagct acaatgttgc ttacaaaatc cgacagtata
    68161 tatatggaag caaagtcatt agctttttat ttagacacaa aaataataat tagcgatcaa
    68221 tgtgctataa gcctacattt tggcgaagac tctatatatc atccttactt aacttttaaa
    68281 tattatatcc acccacgttt tttagaatta attcgttcta gagaaggtat gaatcaagtt
    68341 aattattcaa actcatacca ccaaatcaat atggatttca cttggatgaa atggtttgtt
    68401 gataaataca aaattgagtt tacaacaatg agaactgcag attctaaaga accaaacaaa
    68461 gctcttttcg agtcggcaga ttattttcgt ttagaaagat atagagatat tcagaaaaaa
    68521 gatgcacaac accctcttgc tgttattaca aattttattt cttctttttg gggaagtaat
    68581 gattttcaat taaacgactt agcaaaatgg atgggttata gtccgcaaca agtagtgcaa
    68641 atggttttag acttagcata ccgtggattt ttaaattacg accctcaaag ccaagaaatt
    68701 atagtttatc ctaatgcttg gacattttta caagcatata aaaaccaaaa agattctgac
    68761 gttattcaat tttatagcgt aactgacagt aataaaacaa atgcagaatt tagtttgata
    68821 aactttgatt taaaaattga tggaattcat gaaatacatt tgtctgattc tcaaaacata
    68881 agagttttcc ctaacaataa aaaaattact cttcaaaaaa atagaacctt tacttttgat
    68941 ggaacaatac aagccgggca attttatttt tacggtagca actacaaatt cgactacaac
    69001 agatttatga tagaattaaa tcaatgtgat tctatgaaaa tggttgcaga aacagacttc
    69061 ttagattcag atggaactta caaacctgct attgttagaa ataaattaga acaaataaat
    69121 ggagagtttt atattgacga accattgaat aaatctggaa gacaagattt cccagaatat
    69181 cctaaattta tttcaaacga aaaaacttat gtttactatg atagacccga tgtttataac
    69241 ggaatttaca aacgaaacaa tttttatttt gaagttgacc catttgagct agacagtata
    69301 aaaggttatt cgagagacaa tttgcgattt aaaggcactc tttattctgc cgatattttt
    69361 ccacctataa aagaaacttt agtattacgt aaaaatgatt tctcacttgg ctttaacaca
    69421 agaacaggac ctgttgggtt accgctatac agtggcaaag caacattttg gcatgaaata
    69481 gatttaagta accaaggact tcgtgggtct ggaagaatag aatatctcgc tgcaaatatg
    69541 aatcctgatt atttgatatt tttcccaaaa ttcatggaag gacatactcc aaattttgaa
    69601 ttgaaaaaac aatcagcacc tatagaatat cctaaagttg aaggaagaga caatacttta
    69661 agatggaatg ttgaagatga tgattttatg attaaaagag acacctccaa ctttacaatg
    69721 tacgaaggaa aaggaagttt tgaaggagat ttgaatttaa aatcttcagg acttgaagga
    69781 gatggaacaa tgtttataga taaagcaaaa atagcatcag agttaataag ctttaagcaa
    69841 aatgtttttg attctgacac tgcaaatttt gacctttaca caattaacgc tctaaatgtt
    69901 gattttaaat cagataacgt aaaaacacat atagatttta tagacagaaa aggagtgttt
    69961 aaatcaaacg gcgaaaatac tatttggact tttccaaaaa acaaatatat gagcgaaatg
    70021 aacgagatga cttggtatat ggataaagaa gaaattgcta ttagtgccga cacagacgtt
    70081 ttagaaaagt tagaaaaaga aggagcattt cttgaacctg acgaatggga agcaatgttc
    70141 ttagaaggtc ctaaatttat atctattcac ccaaaacaag actctctata ttttgttgct
    70201 ccaagtgcta gatatgatta tataaatcat attatttatg cagaaggggt taaatttatt
    70261 aaagtagctg atgcaacaat atatactgaa gatggaaatg taacaataga aaaagacgct
    70321 gtaatgcaaa cattacaaaa tgctaaaatt attgccaact ctacaagcag acatcacaca
    70381 gtctataatg catcagtaaa tatttatgga aagaaaaatt ataacgcttt tggagattat
    70441 gattttataa attctactaa acaaccacaa actattcatt taacaaaaat tgcagttgat
    70501 gcttcgggac aatcatatgg tactggcaca attttagaac ctgacaattt tactataagc
    70561 ccatattata aatatcaagg agatgtaact ttttatgcaa ataatcctga gctagaattt
    70621 aatggtgctg caaaaattat tgatgactgc gacacaacga tttttggttg ggtaaaattc
    70681 aaatctttcc tcgaccctga agatattttc ataaaaattg attccataac atacaatata
    70741 aacaacacaa gattatgttc aggtttagtt ttagcaaata ccaaccaact atatcctgca
    70801 tttttaacaa gaaaacgaaa ccaatacgac caagaaattt tttctgccag cggttatttg
    70861 cactacgacc aagatgacgg aaaatttatt atcgcaccaa aagaaaaaat caccgaaact
    70921 tctttgccag gaaatttaat tagacgacat aaatctattt gtaatgttta tggagaagga
    70981 aaatttgaat tcagcaaaga tttcggactg tttaaaccta acgccattgg taactttatg
    71041 tggtacccag aaaccgatac cacagaaata aatgtaagta tgattgcaga tgcttacttt
    71101 agtaatgccg cattaaaagc aatggcagat aaactaaact ctacaggagg cttgtcagga
    71161 ctaaatgtaa gagatgatgt gtttgaaaaa gcactaatag aatttttagg aactgaaaag
    71221 gctgaccaat ggttctctaa tctaagttta ggtaattaca ataaattccc aaaagaatta
    71281 gaaaacaaac ttatttttac cgatttacaa ttctattggc atagagcctt aacttccttt
    71341 gtacattatg gacctattgg aattgctaat ttaggtaaag aacaagtaaa caaatatgtt
    71401 ttcggtttta ttagaataga aaaatctaga cgtggcgatg tctttgaaat gcttttagag
    71461 cctgattatt caacttggta ttatataaga tatagtgctg gaacatttac cattatttct
    71521 tcagacgaaa actttaataa tattgtttac aaccaaaagc ctaatcaaag agaaaaaacc
    71581 gaaaatggtg tcttttatca atacggctta ggaagttctt cgtatatgaa gagatttaaa
    71641 aaagatatgt acaaattttt caaaattgac gaaaacataa atcttgatga ataaagctaa
    71701 tatatctatt tgcgttatta tatgtataat tataatacaa gcttgcgttg gagtacagcc
    71761 tcaaaaccag acttcacgcc caagttcgta taaatataat tttgcctctc gttacaaccc
    71821 ttcttcagca gaaatacagc ccgaagtaag aatttttgca aaatctaaaa ccgaagcttt
    71881 agttttttac aaaattccta ctaacgaaat ccaaaaaact gtaaacacta ttgatgatga
    71941 tgctagacta attgtaaaat atactttaag aaatgctgat aatttcgcca ttgttgatag
    72001 tggaacggtg gttaacatga ttaatcttaa acaaacaaat gaagagttta attcatattt
    72061 tacagtaaat actccaaatc aagaaaatta taaaataatt atcagtattt acggcgaaaa
    72121 agctaataca gggaaacgac tacttgaaga cattgaacta aagcaaaata atgaagcatt
    72181 tttcttacca aaaattttga aaaacaacga agaagaagtt ttatacacta actttattaa
    72241 ctcaaataac acatacaaac ttagtagcaa tatttgtgga cctattgtaa aaattgatta
    72301 ttacaaaata accgaacatg tacatttacc accttattat attgctcctt cccaaaatgt
    72361 tgacgcaaca ccagattcca catttacata ctcattaaac gacagtataa aatttaatga
    72421 aaaaggaatt tattttgttc gcgaacaaaa tcaaagttct ggtggactat gcttaattaa
    72481 tattgacgac tattttcctg aaattaaaac tgtggaatca atgttagaac cacttaaact
    72541 actaactacc aataaagaat ataaagagat tgcaaattca gaaaatttaa aacaagctat
    72601 agattatttt tggcttacta aatctcaaag tcagaaattt gcaaaagaac aaatacgggt
    72661 tttttacaat agagtagctt tggcaaataa atattttagc gaaaataaag aaggttggaa
    72721 aacagataga ggaatgattt acataatgtt tggaccacca tcaattataa atatcagcac
    72781 aaaccaagaa gagtggtttt atggcgaaga tcctgatgtt gctggaatat tttttctgtt
    72841 tactagaatt gagacagcta gctataataa atcttggatt ttacatagag acgcaaatta
    72901 ccaaactgtt tgggctcaag cactacagat ttggagaagt ggaaaaatat ttacaattac
    72961 aaattaaaaa aatggaaaat tttatttttg gactacatcc tgtaaacgaa gcaattaaat
    73021 ctggaaaaga aattgataaa gttttggtta aaaaaaattt aactggaaat attgcactaa
    73081 aattagtttc agaaattcgt caacaaggaa ttactataca atatgtgcct caagaaaaac
    73141 ttgataaaat ttgtaaacac aatcatcaag gcataattgc ttatgtgtcg ccaattaaat
    73201 accaaaattt agaagaagtt atcatcaaag ttcaagatga aaataaaacc ccattaataa
    73261 taattttaga tggagttact gatgtaagaa attttggagc cattgctcgc tcttgtgaat
    73321 gtgcgggagc ttctgcaata gtaatcccaa caagtggcag tgtaagaatt agcgacgaag
    73381 caattaaaac ctctgctgga gcattgtata atattccaat ttgcaaagtt gaaaatttaa
    73441 tagatgctgt tttgctttta aatcaaatga atatcgaagt tgttgcagtt tcagaaaaag
    73501 ccgaaagatt aatttacgaa ctcgacctaa caacagcttg tgctttaatt ttcggtgccg
    73561 aagacaaagg aatttcaaac tctcttataa aacgagccga caaaaaagcc aaaatcccat
    73621 tatatggaaa aacatcttct ttaaatgttt cagtgtctgc
//
LOCUS       DAOPNX010000002        70034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_497_length_70034_cov_6.485217, whole genome shotgun sequence.
ACCESSION   DAOPNX010000002 DAOPNX010000000
VERSION     DAOPNX010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 70034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 70034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70034
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_497_length_70034_cov_6.485217"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3305)
                     /locus_tag="PKN53_00285"
     CDS             complement(<1..3305)
                     /locus_tag="PKN53_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOD87424.1"
                     /translation="MKKLIFLIVFLGFSLFLFSQQSEIIPLSVNHNKLEFKGFTPTAF
                     SAKVQMKELKATNKVIENREFASIDLEGFASTGTVGQAGVPTLSRMIEVPHGAEVQVL
                     IHGYSQEVINLSEYGINRIEPVQPSYSKSTPIEEQYFVIDENYYNTDLYDTEDIVKVE
                     IYGTMRGVRIAGLTIRPYHYNPVENTLIVYNDLDFEIKFVNADLALTEEMKNRYYSPE
                     FAGAYSNLINFSAPAAKDQLTNFSAPLKYVIVANQAFQTTLQPFVQWKTKQGFNVIEH
                     YVASGTTNTTIKNYLQGLYDAGTASDPAPIYCLIIGDHSGTYSIPAFATKVSGESHIT
                     DLYFACFDGTSDYLPDMYYGRISAESTTELQNALDKILPYQQYSLSNFDYLDKALLVA
                     GVDPYWANKCGNAAILYATNNYINTAHGFANIYAYYYNTTSYPYSVMRSNSSSASGDI
                     NSKIGAGVGFGNYTAHCDHDGWADPAVLRSNVPDWANKNKYPFLIGNCCLSFQFNKSD
                     AFGEVLLHTKDAGAVGYIGTTNYSYWYEDTYWGLGLVSQATVDAGNITAYNTSNTGLG
                     FMDGVFHEHYGDDHANWYYTASQIITCGNLAVQASSETSRKQYYWEIYHVSGDPSLIP
                     YMTKPDPLTLTFTPPFVGATTLTVNTEPYTYVAISKNNVLLDAKWSGSGSTVTLNFPA
                     LTGENHCVVGTKQDRAPYINESVLPTVPSVPVANFSASPTTILEGETVSFTDASQYAA
                     SWSWNFGDGQTSTEQNPSHVYTAAGTYTVSLTVTNTLGSDTETKNNYITVNINTNPPT
                     ADFTASQTNIIIGTTVGFTDLSINNPTGWSWEFEGGTPATSTEQNPSVVYNTPGTYKV
                     KLTASNTYGSDVEEKLAYITVTLPTYCSAGSNSASYEGISNFTCNTINNSSGKSTYTD
                     FTHISTDLLIGQSYPFSITTANGYTSDQVLIWVDWNRDGDFIDDGENVFSGNGQGPFS
                     GNITVPSTVTQGEVRVRIRLHDTGSSYNPNATPCGNSGYGEVEDYTFNLISPNIPPVA
                     DFAISGPAFCSGEVSFTDMSSLADSWLWSFGDGNTSVAQNPTHSYASSGTYTVSLTVT
                     NGYGNDQKIVNDAV"
     gene            complement(3465..3932)
                     /locus_tag="PKN53_00290"
     CDS             complement(3465..3932)
                     /locus_tag="PKN53_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87425.1"
                     /translation="MKVLSIINTLLIISASFIALLSLKACADDDYITSVDSETIDCQY
                     CYEDMPELVDLKLVFNMKKYKEKVEFIVFSGYAFTSAVWMEGNTDVNELWIQVKPNQK
                     YTVVAEYFDGDKTIYVINDALVKTKFFKTGCDKPCHYVYEVSCDLKLGEHKYK"
     gene            complement(3892..5298)
                     /locus_tag="PKN53_00295"
     CDS             complement(3892..5298)
                     /locus_tag="PKN53_00295"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOD87426.1"
                     /translation="MKKYLLAYLLSILFLNLFSQSEEYYTDEYYYYGDRLLYCAYNGD
                     LEEVKEILEGGKTSVNFVDYYGVSALMFAAEQGHTSVVEFLVQNKADLNLASFENGNT
                     ALHSAVKFNHLKIVEILLNAKADINTKDFDERTCVHYASIYGYDAILDLLLSNEAEAD
                     LKDDYGFTPLMYAVMYNNNSAVMLLRLHDADVDFVLSDSSNVFHLAAENNNIWYFENF
                     RNEIDLKENIYGLTPIESSIIYGQYEMLKWFLENNYELRDTINSAYTPKTLAKYGGDR
                     NIKKIIKKLNIKDYNYLWFDRLALSQGMIFSKNDFFWTTGLGVLDARCGLEFETGFFY
                     RHFEKLVLLPILNNHSYYQLRESRKGIYLIGLKNFNLLRSKNNFYMDFYAGLRATKWW
                     GKYDGVEFKIIDGIIASPFIGISLHNRSNCKFKISTEYLTTNIYAQSKFFYSLSLSLL
                     VNFRKNESLINHKYIINY"
     gene            5370..5441
                     /locus_tag="PKN53_00300"
     tRNA            5370..5441
                     /locus_tag="PKN53_00300"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5403..5405,aa:Arg,seq:ccg)
     gene            complement(5700..6653)
                     /gene="gldN"
                     /locus_tag="PKN53_00305"
     CDS             complement(5700..6653)
                     /gene="gldN"
                     /locus_tag="PKN53_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HOD87427.1"
                     /translation="MKIVNKFFPLIVLISLCLPISAQVLDGAYVREHVTEHTFVPYQN
                     IREADAMYSKKILRVLELREKQNHPLYFPIVRMTYPGTGLDNEGRPVKIQPQRSRVNL
                     IYLMYNVGILGEQYSDITGELTLIDTVRRYPVYKLDAADITNWWRHPIEKDDELREHL
                     LAYKEKILVPDGEGGTYEETIDSKLDDTRVLDKLWVWEEWVFDKQRSLMDVRIIALAP
                     DGWVVKDETVERVWPFWIYFPDYRPLFARYEVFNSHNDAEHRTFDDIFFKRKFSSYIV
                     AESNNYDNRLISDYLLGIDAIREGERIQEKIALIEHDLWEY"
     gene            complement(6671..8302)
                     /gene="gldM"
                     /locus_tag="PKN53_00310"
     CDS             complement(6671..8302)
                     /gene="gldM"
                     /locus_tag="PKN53_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HOD87428.1"
                     /translation="MAATNCPETPRQKMIGMMYLFYTALLALNVSSEILTSFVKINES
                     IQKTTSNYSEKTKSLYAKIDNSALEQPGKYTKLAQQAHEVERQSNEFIQNIEDLKLLI
                     LRESQGPEATLDGEIKKMDDLHAATIVMVGEGGPMMGKQLRGWLDSYRELLLGIIEDT
                     SLTVYKNVAKNLEAKDQFDNKEAPWSWEETLCRGMPMIGTMAMLSKLQADIRNSEADV
                     LEFMIAELEGLDIRITALEALVSSPRSFVIRGGKYSSNVFLGARDTSMRPTIYLTYNA
                     PFYDSTVVNGEVQYKLRQGANYDTLPIDESGKGLHVRDCGGVGDFNYGGLVHYKSNKG
                     DMWLPYKAVYQVGDAGFTVSASACNVFYRGLDNPVEVAVSGYPQEAVSVSISGGASIS
                     RSGAGYIVKVPASVSAREVSITVSVRTEEGVRTLGSKTFSVLNVPPPTIVIAGAYKDG
                     ASVPKGAITRTPTLTANLESDFFPFKGVSYSVISYEFLYSVRGVTQTIAGTGSNIPQT
                     ILNNLNPGSAASFTNIRVSSPSGERKTNGITVKIQ"
     gene            complement(8348..9274)
                     /gene="gldL"
                     /locus_tag="PKN53_00315"
     CDS             complement(8348..9274)
                     /gene="gldL"
                     /locus_tag="PKN53_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HOD87429.1"
                     /translation="MSFSELFVSKGWKKFMGFIYGWGAAIVMVGALFKIEHLPGATIM
                     LTVGLLTEALIFFLSAFEPPHVEYDWGLVYPELDGIEENLNVLDKKAAISSKKSALEK
                     FDAMIENAEITPELFEKLGHGLQNLNNTAEKLQDVSDATVATNNYVANFEKASEKVSS
                     FADAYGSSAERLKVEADKLARTYEKSASVVGDSGEQLASTYNKLIENMNKELEMSTSG
                     GKTYNEQLSIMNKNLTALNAVYELQLQGTNQQIEATKAMYTGLDEILDNMKQSAEDTR
                     KYREEISKLSHNLAAMNTIYGNMLSAMNFKAD"
     gene            complement(9354..11012)
                     /locus_tag="PKN53_00320"
     CDS             complement(9354..11012)
                     /locus_tag="PKN53_00320"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOD87430.1"
                     /translation="MRKLLIISAVIIVSLSSCRYYGSGELTGIDRKVWYTPDPYGMLF
                     IPPGSYQMGPSDQDVPYSMTAQSKTVTVQGFWMDETEITNSEYRQFVAWVRDSMALTV
                     LGKETKNSVDFGDISTKKSYQPIDFIIDDTDVTEEESNFITNMYENGEFDFDDYDNYN
                     FLDWRGPLDYNDLEIKGILIKNEIPNNFNQTLYLLPPAELYHFMRQTDLNTDALYYSY
                     FWFDYKQAAQKHTFRPGADGVDQDVARTFSREGDHGLRIGRHFNPETGNYEGKIDDRE
                     KLDEDRPWDYFADREIKNYDGKTDAGRRGGRYSYWIREKVHIYPDTLCWVSDFTYSYN
                     EPMTKMYFWHPAYDYYPVVGVSWTQANAFCMWRTMLKNAYQRTVGEYHLNDYRLPTES
                     EWEYAARGGLDLSMYPWGGLYTRNYSGCFIANFKPMRGNYYDDGGVQTLMVATYDPNE
                     WGLYDMAGNVAEWTSNAYDESAYSFTHDLNPDYQYYAHPDDPPAKKRKVVRGGSWKDV
                     AYYMQNGTRTYEYQDSAKSYIGFRCVRPYLGRHRGDGTSYSQVY"
     gene            complement(11064..12017)
                     /locus_tag="PKN53_00325"
     CDS             complement(11064..12017)
                     /locus_tag="PKN53_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOD87431.1"
                     /translation="MRKVLLLIVFFGSIFVKYSQAQSDPHFSLFEYSQNIYNPGAAGS
                     NNAMCVTSLHRQQWVGFKEEGRPVTTLFLFDMPIPSLDMIGVGLNIQQDIIGFQKDLN
                     VSANVAYRLELDFGMLGVGVGAGIINRSINGNWRTWGSLIGQPVYIDPTIPHMESKTA
                     FDLNFGATLVGEKFWVGLSSTHLLNPVIKFDIDNPSYIARHFYLTGGYEYSLPNPSFD
                     LIGTGMVQSDGSGFPEIQINGKILYNKSFWGGLSYRYNDAIVPMIGVHLVNGLSFAYS
                     YDISLIKIGSNMLGSHELMVRYCFNLSMDKTPGRHRGVRRL"
     gene            complement(12355..14628)
                     /gene="topA"
                     /locus_tag="PKN53_00330"
     CDS             complement(12355..14628)
                     /gene="topA"
                     /locus_tag="PKN53_00330"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOD87432.1"
                     /translation="MSTNLVIVESPAKAKTITKFLGDDFVVKSSFGHIRDLSKKNLGI
                     DIENGFLPQYEVPADKKKIVAELKKHVADAKTVWLASDEDREGEAISWHLKKVLNLKK
                     DEVKRIVFHEITKEAIQNAIKNPRDIDENLVNAQQARRVLDRLVGFNLSPLLWKKIKP
                     ALSAGRVQSVAVKLIVEREREINNFVSKSYYKIIGIFKVDKNTIKAELNKKFKTLEEA
                     EAFLETCKTEDFVVSDITKKPGKKSPAPPFTTSTLQQEAGRKLGYSVGQTMKLAQSLY
                     EHGFITYMRTDSVNLSNLAIGSSKKVIEDTFGAEYVKTRKYTTKTKGAQEAHEAIRPT
                     FVANETIEGTKQEQKLYDLIRKRTIASQMQDAQIERTIITLNNSNDTISFVASGEVVV
                     FDGFLKLYIESTDDENMDETQSILPKVKKGDKAIVSKIEANQKFEQPPYRYTEASLVK
                     KMEELGIGRPSTYAPTISTIQQREYVIKENRSPKTRSLVNLVLENGKIEKKKEEEKFG
                     GEANKLFPTSMGIVVTDYLENHFDNIVSYDFTAKIEEEFDKIAVGEIAWNKMLADFYE
                     DFSKQVEDSLKEKETTKWEREIGIHPETGLPIVLKIGKYGPYASIEGVEPPRHASLRK
                     NQNIESVTLEEVLDLFKLPRNVGTYEEDDLIVAIGKYGPYVKNNGKFYSLKKQDDPYT
                     INKERAIEIINEKKSQQKEAFGRTFEEMPGLTILKGRYGPYISYEKKNYRIPKTYDPE
                     TMSLEDCKTIISQSKKV"
     gene            14721..17072
                     /locus_tag="PKN53_00335"
     CDS             14721..17072
                     /locus_tag="PKN53_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOD87433.1"
                     /translation="MMENKNAKVTFSKKFTKKFLITFWTCIGIFIVGLFIYFLLLSKG
                     VLGYMPSFEELENPKENLASRVYSSDLVLLGTYFRENRSNVSYDELSPHLIDALIATE
                     DVRFHSHSGIDFKALPRVFSGILTGNKKGGGSTISQQLAKMLFPREERLSKFKMVNRK
                     FQEWVIAVKLERNYTKEEIIEMYFNKFDFLNLAVGIESASRVYFDTIPIDLSITQAAM
                     LVGMAKNPALYNPLRRPEETLQRRNVVLNQMRKYGKITREEYDSLKQCPLGIRYQKVD
                     HNIGSATYFREFLRAWLSATEPQPNDYIDQRDYIEDSINWHTDPSYGWCNKNVKPNGE
                     NYDIYSDGLQIYTTIDSRMQKYAEEAVKEHIGGTIQPEFFKDQKYNKNSPFTNDLTKD
                     QIEKIMNASMRRSERWRVLKANGMSEDSIKLSFSKPEQMRVFSWKGEIDTVMTPLDSI
                     KYYKTFLRASFMSMEPKTGYVRAYVGGIDHKHFKFDQVTKSRRQVGSTIKPFIYCLAM
                     QNGYSPCTQVPNVEVTFKLPEGSAEEYYTPKFSTSKLDGEMVSLKTGLALSLNQISAW
                     VLKQFSPEAVVSIAKKLGVNSHIDPYPSICVGAPEVLLSEMVAAYAAFANGGLYTRPI
                     YVTRIEDQNGNVLATFGQIQTQALSEETAYLMIELMKGVVNEGTGSRLRFRYNLNSEI
                     AGKTGTTNNNSDGWFIGITPELVNGAWVGGEERSIRFRHMTFGQGASLALPIWGLYMQ
                     KVFDDKKLPYNSKATFTRPNEVSVETDCAEYNRNHTNINFNEL"
     gene            complement(17161..18504)
                     /locus_tag="PKN53_00340"
     CDS             complement(17161..18504)
                     /locus_tag="PKN53_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOD87434.1"
                     /translation="MAKKNVIIIGAAGRDFHNFNTYYRHNPNYNVVAFTAAQIPDIAG
                     RKYPKELAGEDLYPNGIPIYDQKDLEKLIKDLNVDECVFAYSDIEYRHIMNIAAMVNT
                     AGANFVLLGPKATSIKSTKPVIAVGAIRTGCGKSQTSRRIIEAFMELGIKVVAVRHPM
                     PYGDLNAQRVQRFATVEDLKKHNCTIEEMEEYEPHVVRGNVIYSGVDYEEILRAAEND
                     PNGCDVILWDGGNNDFSFYETDMMVTVVDPHRAGHELSYYPGETTLRLADVVVINKID
                     TASPEGIQEVRDNINKVNPNAIVVDAASPIAVDKPELIKGKKVLVVEDGPTLTHGDMT
                     IGAGTVAAKKYGAAEYVDPRPYTVGKLTTTFETYPGIGKLLPAMGYGAEQIADLEKTI
                     ANTPCDTVVIATPIDLSRVVKINKPTAKVEYELQEIGQPDFKSIIKDFVAKYVKK"
     gene            18779..19483
                     /locus_tag="PKN53_00345"
     CDS             18779..19483
                     /locus_tag="PKN53_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOD87435.1"
                     /translation="MQIKKSKKADLESRKSTFFLIGLSVSLALLLLVFEWTSKDVVAS
                     SLGSLGASDIIEEEIESTVQEQEQPPEEVEQPEQEPETIIEELTVVEDDVKVDDININ
                     TEADEKTQTSTRVVQVTEVEIVEEEVEPIAFAVVETKPEYPGGEVALLQYIAENTVYP
                     PVAKENGIQGRVFVQFVIDATGRVTKVQVARGVDPYLDTEAVRVVKTLPKWTPGKQRG
                     KSVPVTYIVPINFKLY"
     gene            19548..20732
                     /gene="hflX"
                     /locus_tag="PKN53_00350"
     CDS             19548..20732
                     /gene="hflX"
                     /locus_tag="PKN53_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOD87436.1"
                     /translation="MINKNFYISEEDAVLVGVATQYQTKEEVQEHLEELDFLAKTAGV
                     VPKKYFIQNLDLPHQKTYVGSGKLLEILDYITKNEIKLVIFDDELSPTQLRNIENVLQ
                     VKVLDRTNLILDIFAQRARTSHAKAQVELAQYQYILPRLTRMWTHLERQRGGIGMRGP
                     GETQIETDRRIILDKISKLKEELKSIDKQFTQQRKNRGQMIRVALVGYTNVGKSTIMN
                     LLSKSDVLAEDKLFATLDTTVRKVVINNLPFLLSDTVGFIRKLPHKLIESFKSTLDEA
                     KESDLIIHVIDVSHKSFQEQITVVDNTLKELKINDKPILMVFNKIDAFEYSPQDEFDL
                     TPKTKENYSLEDFKNMYFAKTNNPAVFISAKNKENIEEFREVLFNLVRDLHFERYPYN
                     TI"
     gene            20823..21482
                     /gene="upp"
                     /locus_tag="PKN53_00355"
     CDS             20823..21482
                     /gene="upp"
                     /locus_tag="PKN53_00355"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOD87437.1"
                     /translation="MKLNILGGRGSLLDQFVAEIRDTQIQQDRMRFRTNLMRVGEIMA
                     YEISKNLEFIDEEVTTSLGIATVPTLKQQPVISTILRAGLPFHQGFVNYFDHADNAFI
                     AGYRKYNKNGTFEIKIDYITAPSIEDRIVIINDPMLASGGSIELAYKGLLNFGKPKHV
                     HVATVIASKDGLDYVDKILPNNMITVWTIAVDDELTVKSYIVPGLGDAGDLAFGPKIN
                     Y"
     gene            21571..23322
                     /gene="aspS"
                     /locus_tag="PKN53_00360"
     CDS             21571..23322
                     /gene="aspS"
                     /locus_tag="PKN53_00360"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOD87438.1"
                     /translation="MYRTHNCGELRSKHIGQKVKLSGWVQLKRDKGGMIWIDLRDRYG
                     ITQLAIEEANTSKEIIETIDKIGREYVISVTGEVIERYSKNDKMPTGDIEIKVEEIEI
                     LNESETPPFTIIDNSDGGDELRMKYRYLDIRRNPVKNALLLRHKMAQEIRKYLDEKDF
                     VEVETPFLIKSTPEGARDFVVPSRMHQGEFYALPQSPQTFKQLLMIAGMDKYFQIVKC
                     FRDEDLRADRQPEFTQVDCEMSFVNQNDIIATFEAMTKHLFKTIKNIDIDYDFPQMTY
                     KEAMEKYGNDKPDIRFGMTITDITNLVKGKDFVVFDSAEYIGAIALENASEYSRKQID
                     ALTDFVKTPQIGAKGLVYCKYSTDGSFKSSVDKFFNENDWTKISQAMQAKQGDLILII
                     AGEKDASLHALSELRLKLADENGLRSKDNFAPLWVIEFPLLEYDKETDRFYAKHHPFT
                     APFDEDIKLFDTDPSKIRAKAYDLVINGVEIGGGSIRIFNKDLQALMFKNLGFSEEEA
                     QEQFGFLMNAFKYGAPPHGGIALGFDRLVSMFGGSDSIRDYIAFPKNNAGRDVMIESP
                     STISQAQLDELGLIIKK"
     gene            23439..25604
                     /locus_tag="PKN53_00365"
     CDS             23439..25604
                     /locus_tag="PKN53_00365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOD87439.1"
                     /translation="MAKQKTKHNLKQKPLPQQEIKKVTKNKKYNLFVFLTISVFAFIL
                     YGNTLSHDYALDDAIVITENTFTQKGLAGISDILKYDTFVGFWLTKNPNKTAEQIQEE
                     KKLVAGGRYRPLSLISFATEIEFFGKNIKNDAGKVVYKENPFVSHLGNIILYLLTTYL
                     LYLILAKLFPPDENKKWYLSFPFIASLLFLAHPLHTEAVANIKGRDEIMTLLGSLGAL
                     WFTLKYLDTKKAKFLVYSSLSLFLGLLSKENAITFLAIIPITVYYFTNHNLKSNFKSL
                     LPLLLVSGVFLLIRGSVLGMGTGEKQIAQEIMNNPFFGASVGEKFATIFFTLWMYIKL
                     LFFPHPLTYDYYPWQIEIINWSNPKAFLPLILYLALGIYALYGLIKKKDIFSYSIWFY
                     LIPLSVVSNIFFPVGTFMNERFIFISSIGFCIFIAILLTKYLNKLIKNTQTHSYFLVL
                     IAVSILGLYSFKTIDRNKAWKNDLTLFTTDVKTSTNSAKSNCSAGGKLIEEAQKPMYK
                     NDTATRNQMINQAIVYLERALEIYPEYIDAINLLGNAHYELNYNINKALYNYAKILKL
                     KPYHSIAYGNARIVLLNTIGLLNSQATVSTPDDVIAGCNEVLKAHPDFGEANHIMGTM
                     YGKYKNMLDSSLFYLEKANQCNFNKDAGFYRDLGVAYGMSGIFKPALDNFLKAMELDP
                     NDYQTYMNIGVSYQQLGDIANANKYYLKAQEFQNINTNQ"
     gene            25601..26359
                     /locus_tag="PKN53_00370"
     CDS             25601..26359
                     /locus_tag="PKN53_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD87440.1"
                     /translation="MIKDKKIVVVLPAYNAELTVESTYNEIPFDIVDEVILVDDKSKD
                     KTVEKAKELGIKHIFIHDENKGYGANQKTCYNNALKLGADIVIMLHPDYQYTPKLIHS
                     MAYLIANDVYPVVLGSRILGKGALKGGMPKYKYFFNRILTFSQNILMNQKLSEYHTGF
                     RAFSADVLKKINYNINSDDFVFDNQMLAQIFYAGYEIAEITCPTKYFEEASSINFKRS
                     VKYGMGVLTTSLKYFFNKLKLTKSDIFKPIASEE"
     gene            26359..27303
                     /locus_tag="PKN53_00375"
     CDS             26359..27303
                     /locus_tag="PKN53_00375"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOD87441.1"
                     /translation="MQSLDKILDIKYPVILAPMFLVTTPEMVRIALDCGITGAIPAMN
                     FRKESELKAAIEDLKKHSDKAFGINLITNRSNQKYKKQLKAILEAKPAFVISSLGNPK
                     ELITEAHKVGIKVFCDVVDLKFAKKVEDLGADAIIAVNSSAGGHSGIIERDELIGQLV
                     ANCQIPIINAGGVSTNSDLNHVMSLGAAGASVGTIFMASHECHISDDYKQALIKYGKD
                     DIVLTRKLSGVPSCVIKTEYVKKLGTKPNILELLIKNNTFLKKYAKYLITNFGMKKLE
                     KAAFSATYKNFWVASKAIENIKEIQNLSVIIKKLVQPK"
     gene            27358..27771
                     /locus_tag="PKN53_00380"
     CDS             27358..27771
                     /locus_tag="PKN53_00380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOD87442.1"
                     /translation="MKKLKAIFILLFVASISIYAQNYDDPQNIDLQYNRDAEYPGGMN
                     KFITDLWNSMSYTQEAIDARVDGTITISFDVEANGEVSKVDVIGGLGMGIDEEVANVI
                     KAMTFTPALVEGKAVKMNLMFTVPIRVGPNSRLKK"
     gene            28192..29565
                     /locus_tag="PKN53_00385"
     CDS             28192..29565
                     /locus_tag="PKN53_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87443.1"
                     /translation="MQEDLNGQVYIDEGACSISDTAGNLLFYAGPNGGTLPGENNMTV
                     YNANHEVMLNGTDIDCGWSGRQNSIIVPLPGNDNIYYLFTIGDVAVVEYNMDQPGFKY
                     NIIDMSLDGGLGAVTEKNIEIYSNQANDTLSEILTAVHHANCEDVWIVIHNYMTDVFM
                     VYKLTADGLDSNVVINQIGNSTWGNYHTWGWGMKFSPNGKYAAVNKNFMCPSNCQIKD
                     TLELYRFDNETGILSDRIAIPMDSAVAGKEFSFTNDLLYTFEAHIYELEIPPSIYQYD
                     ISKWNKSYIMDSKTLIGNYFFPTPDLHLTPLNTIIISGELAFNPPEGYIGVIENPNVR
                     GIGCSINENAFYISRNPGWNFPNFVRSYFNTDISVYNCNTVTVEDVIKNQAFEIYPNP
                     FSKQAEIKTYDKEIIYYDLFDLKGNKLLKDKDYKLRQIDNAYIFVNETLKEGIYINLQ
                     VLRQQIF"
     gene            complement(29758..30471)
                     /locus_tag="PKN53_00390"
     CDS             complement(29758..30471)
                     /locus_tag="PKN53_00390"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOD87444.1"
                     /translation="MEERINEQVNTMPGLNAPAPEFTANTTQGKINFPADYKGKWVIL
                     FSHPADFTPVCTSEFMTFASLEEKFNKANCELVGLSIDGLHSHIAWLRTIKEKIEYKG
                     MKNVEVKFPLIDDISMNVSRKYGMIQPDESATQAVRAVFFIDPKGILRAMIYYPQSLG
                     RNFDEIYRALIALQVSDEFGVATPADWQPGDDVIVSPASTCSAAQARFDEKDLDCKDW
                     FFCTKKLDKDTILKKIIKK"
     gene            complement(30509..30691)
                     /locus_tag="PKN53_00395"
     CDS             complement(30509..30691)
                     /locus_tag="PKN53_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87445.1"
                     /translation="MLDTRQEKPHHLYEIDSGKIEDYFDDELNSILEEYFRNKTIPGF
                     EIKDVKLQIKGKYISE"
     gene            complement(30678..30932)
                     /locus_tag="PKN53_00400"
     CDS             complement(30678..30932)
                     /locus_tag="PKN53_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOD87446.1"
                     /translation="MNTNNYISKLKETGLKVTPQRVAILKVLNELNHPCADEIAEALK
                     KELPGLSLATIYNNIETLVQKRLVSKVLSEDGKFRFDARH"
     gene            complement(31088..31663)
                     /locus_tag="PKN53_00405"
     CDS             complement(31088..31663)
                     /locus_tag="PKN53_00405"
                     /inference="COORDINATES: protein motif:HMM:NF017747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HOD87447.1"
                     /translation="MEMLVIQKQSHKTTNWSGGTTTELFIYPRESEYQKRNFLFRVST
                     ATIEIKESQFTKLDEVNRKIMILDGKIELIHEGRYSKNLDKFDTDTFSGEWNTRSIGK
                     ATDFNLMTKGNVNGHMLHLGFETENTKTVNFNVKTQKALFYIYSGKLQICDKTVESGD
                     VIFLETLSNNKSIDVKAVTKSDIIVVELAGF"
     gene            complement(31676..34549)
                     /locus_tag="PKN53_00410"
     CDS             complement(31676..34549)
                     /locus_tag="PKN53_00410"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HOD87448.1"
                     /translation="MQYKGLTTTEVEQNRQVYGSNLITQHKKRSAFKLFLEKFNDPLI
                     KILLIAALISFGIGFVNDQFIEAIGILFAIFLATGIGFWFEYDANKKFDILNLVNNDT
                     LVKVIRDGFVTEVRKGDIVVGDYVILSVGDEVPADGILIESVSLLVDESSLTGEPSTR
                     KGVDLNEFKKESTFPYNKVLRSSKILDGSGIFVVDAVGDSTEYGKVAKSSVEISNVKT
                     PLNIQLEKLAGFIGVVGTSVAILTFFALLIKDLMLKKISLTFEQSYFFISIIIFFAIL
                     SLKLWFPYFSSFKEMLFKKKSVFLEKVEKIKTIKLFVIALLVLILSVLVSLIFDFHVL
                     EKSAWMDIPTASRILQYFMVAVTLVVVTVPEGLPMSVTLCLALSMRNMLKSNNLVRKM
                     QATETMGACTVICTDKTGTLTKNQMEVVEAMFFGREVLDFNGGDDNLIFIKENISANS
                     TADLDYSDETKIKGVGNPTESALLLWLHKMHCPYEKVRNATEVLEQLSFSTDRKYMAS
                     VVFSPILNKKVLYVKGASEILFHMSKQVYCEDGVKPNSECSDLIKTILAKNHSQALRT
                     LGFAYEILEDDTPRIEDAKLVNHNLTFYGFVGIQDPIRDDVNEAVATCLKAGIDIIIV
                     TGDTYGIAVEIAKKIGLLNADYDEKAVLTGDEFSSLTDEELLLRLKDLKILCRARPLD
                     KKRLVQLLQELGEVVAVTGDGTNDAPALNFAHVGLSMGSGTAVAKEASDITIMDDSFD
                     SIGSAVLWGRTLYQNIQRFVVFQLSINLVAMLIVFIGSIVGHEIPLTITQMLWINLIM
                     DTFAAGAFASLPPNKSVMNNKPRKSNEFIINKTMRNSIFITGLSFVVILLALLYYFTD
                     EDGNISIYNLSLFFTTFVMLQFWNMFNVKALFTGKSALKSLHKSKAFLTVALAILVGQ
                     IFIVQFGGEMFRTVPISFVDWLIIFGATSLVLWIGEIKRLIFG"
     gene            34739..36688
                     /locus_tag="PKN53_00415"
     CDS             34739..36688
                     /locus_tag="PKN53_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD87449.1"
                     /translation="MKIFFKIKVTFIFALVANFAFSQDWTERLKITANERANNALFGA
                     SVAISGDFAIIGANQEKKDADNSEENILTDAGAAYIFKNESGEWIFYQKIVASDRSGG
                     DYFGHSVAIDGDFIVVGAPQEDEDENGQNTKLNTGSAYVFKNIGGNWTEIQKICASDR
                     NTQDWFGCSVGISGNDIVIGAYAQGTDENGGNQTDMAGAVYYFKNQGGTWTETQKMVA
                     HDRRTADYFGASLAIEGNRLVVGAYLQDYNANGYTPKTNAGAVYYLEKENNTWTIKQK
                     LVADDRKAESNFGYSVSISGDYIIVGAHYHDESSSLTSSGAAYIFKYSSNTWEQQTKL
                     IPSDLEANDRFGQSVAISGNYAIVGADLEDHDINGGNNFSNAGSAYLFELQDNNWVQI
                     QKLCASDRSLNDSFGYKVAIQGYNFIVTANQEDHDINNQNYLNNSGSAYIFRKKSNLI
                     VKKDNEIINNGQTLDFGNIVFNDDTLTISLNISTDGGDYICFTNENLFVIQGNNADNF
                     ALEFDKNIDSCLNPVSTINLGVKFIPISQGLKTAQLLLTNDSEQSNFKIILKGNGIAS
                     SSITNISQNISIFPNPASSQIFIDLGKVKVDVLNIYDISGKCLITRTNLKDIINIDIS
                     NLQSGVYILNAISDNEVLNFKFIVE"
     gene            complement(36690..38126)
                     /locus_tag="PKN53_00420"
     CDS             complement(36690..38126)
                     /locus_tag="PKN53_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOD87450.1"
                     /translation="MSPKPGSHIKSFRIFTIVLFVILLILGLFAVLHYFKIGFGIEES
                     KRDYEEIIESKELKVVLATNPIDYFIYQGQPMGFQLEMLESFAKSKGLELVIKIENDA
                     QKGLEMLLDRECDILAQGIINSLSQESYVNYTLPIRSTRLVLVKHVPRNEDILNLKPD
                     TDGNTTLKYIYVAKNQTDIKVLKTYLTEIIPNFYLVEIDSLSNEAIIKNVSEGIFDFA
                     VVDYNIAKISESYWHNIDYSTDVSVSKNISWSLRPESKQLLDSVNFWLEDFLQSNDYR
                     RLNLKYINNNKNLVDLESDFYSISTGKISEYDDKIRKYSKLINWDWRLVSSLIYEESR
                     FNPDLVSWAGAYGLMQLMPVTFNTFKDPEKSGIDAQIYAGVKYIEFLDKKFLEQVPKQ
                     EDRIYFVLASYNIGPGHVEDAMRLAEKYKDDVSSWESVAFYLSNMSNAKYYKDPVVIH
                     GYYPGIYAVSFAESIVKRYKHYQNVIPK"
     gene            complement(38209..40059)
                     /locus_tag="PKN53_00425"
     CDS             complement(38209..40059)
                     /locus_tag="PKN53_00425"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87451.1"
                     /translation="MKKILVLILFVQFAYSLFSQHENIERHFTSYKLYNLPCADDAYD
                     KDTLTDSFFLYLPQQKYSLHPLGLPNLGTAFYPSLFSEKQTHDYWFLNNYIPYIRQHD
                     EIVYFDAKKPFTLFKFAGGGKGQEYVRFLHTQNISPTFNFVFNYDIINSDGYYQYSKS
                     KINAISLATAYTKRKYQSHFNFIFNKINNLENGGLSDIDKYLNSSYSAGTYATNLMQA
                     QNTVSQLGFQYNQEFGFGKYDVDTIVQGKDTSLNKTFDSKFSIIHDLKADRYFRIYQD
                     KPSAYYPNTYFNLENTFDSSAYKVLDNNILLNFNIAQRGKIEKFKVLAGVKSYLYNYD
                     IDTLKSYLYFSNSLIGNLILETEKSYLNFEADYCFIGTDIGDFGVSGKYVLDIGKSTT
                     WQSGVNINSKNIDKFIYHYSSNHYAWENDFYKTQTFAANTNLNFENIKLSTGINTNLL
                     QNYIVFNQESMPVQVAQLNFIADAWLAKRFDFGKFHWLSKLSYQYIKDKSRIPLPDFV
                     AYSSIYFQSPMFKKVMILQLGFDVKYFSKTYLYAYNPALSTFYLQTERKYGNYPNMAF
                     NISVLIKRLRGFVRISNFNSFIMEKNYFVSHKMPDNPFSFNFGISWEFYD"
     gene            complement(40187..40861)
                     /locus_tag="PKN53_00430"
     CDS             complement(40187..40861)
                     /locus_tag="PKN53_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87452.1"
                     /translation="MKKIVLLFIITFCGLIGYAQNWKIGVYYNALNQRVNFLEESSHI
                     FQKNNDFNSFESGIKVEYLLNETLSLKMNVGISQKSSGKVDSSIPIFSYKAVPVGLRL
                     KTDLFNFSNFNIYVENGMSLAFCYSYTYNRIIGSSTSNITGSVSDYNFTNNLSFGFVN
                     GIGISYLIKSKFEIDIFANYYSGINKVWENNSILINEVGGGQNFYTISSNGSCINIGL
                     GLGYCF"
     gene            complement(40939..41631)
                     /locus_tag="PKN53_00435"
     CDS             complement(40939..41631)
                     /locus_tag="PKN53_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87453.1"
                     /translation="MVKSYIVKKIVLLFALCQISFIVFSQNFKVGTYFNVSNQTANYL
                     EKSEFLKPEKAMFFSWEFGLLFDYKFNETFRLKTSVGYSQNHLLFPNFFISSFHYNDF
                     PFSVVSKINVLKINNFSVFIENGLSLAFCHSYNYNRVIETSASTIKGSISDYSFTSNW
                     SFGFVNGIGISYLIKSKFEIDIFANYYSGINKVWENNSILINEVGGGQNFYTISSNGS
                     CINIGLGLGYCF"
     gene            41808..44423
                     /locus_tag="PKN53_00440"
     CDS             41808..44423
                     /locus_tag="PKN53_00440"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOD87454.1"
                     /translation="MKSAFFSKNLPHFIAVAAFLIIASIYSSPYLNGKRISTHDYNTY
                     TAIAKANNDYEKEHNRLVFWNNSMFSGMPTYAVSTAKQENIFFKIYKVLIFNDATPIN
                     MIFWYLLGFYILLNILRVNPWLSMLGALMFGFSSYYFIIIAAGHFTKAMAIGFMGPIL
                     GGVLLAFERKKPWAGMFLMTFFLALQILTNHVQITYYTGLAVIIYGIFELVWAIKEKY
                     LPRFFKTVGILAIGFVLSLGINAAFLMTTQEYIPYSIRGKSELATKQGAGYTSGLDKD
                     YATSWSYGIGETFTLLIPNAKGGASLIPNAKGGASYSALSEDSETHAVIKNVFGKQNA
                     NEICKNMPTYFGDQAFTAGPVYVGAIVVFLFVFGLLIVRGKLKWWLLTLTIFSIVLAW
                     GRNFEWLTHFFLDYVPGYNKFRTVSMILVIAETAMPLLAILSIAEIVKNKIDKRKLMS
                     SFYIALGVTAFVTLIFIISPSITGLSGENKSEVRTAEMFASYFPNEAEYANAKEQFKN
                     DFINAITQDRADLVRKDATRSLVFILLAAVLVYLLINKKLKPNLVIASLAVLVLADMW
                     TINKRYLNDDNFVTKRKYEVPFDKTPADSFILADNDPHYRVCNLSTGATSVFNDGSTS
                     YYHKSIGGYSGAKMRRYQEVHDSVLFREMYFSEYIIREGIKNQFEPAILQQLFDQQAR
                     TPIMDMLNTKYIIYSPEGKPIINKNALGNAWFVDSIIKVESPDEEITKLNSFSPKKEA
                     IVEQRYNEFIKDWKNIEDSTATITLKDVAPDYVVYESKTNSEQLAVFSEIYYPHGWKV
                     TIDGNETSHFRANYILRAMLVPAGKHEIKFEFIPEVYKKGVMISYASSIILFLMMGIG
                     LFFENKKKKQAKLNS"
     gene            44536..45315
                     /locus_tag="PKN53_00445"
     CDS             44536..45315
                     /locus_tag="PKN53_00445"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOD87455.1"
                     /translation="MINKIVILGSGNLAFHFCKAACIAGVEISQIFSRNLETASQLAN
                     MAGCDFTNDINKIDENADTFLFAMTDEANLSIVKKLKPYPDKTLLHTAGSLSMNIFEN
                     ITENYGVFYPFQTFSRNSKIDFSKLPLCIEASNKQTLNALIQLSERLRCNYYIINEEQ
                     RQVIHLSGVFGCNFMNHCISLAEKILEDNKIDKNIIKALLKQSFEKVIENGAFASQTG
                     PAVRRDYEIMEKHAQFLSKDELLLEIYKLMSKSIEETHFKK"
     gene            45312..45851
                     /locus_tag="PKN53_00450"
     CDS             45312..45851
                     /locus_tag="PKN53_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOD87456.1"
                     /translation="MINSLKNLNFKEKLKNLDTLIFDIDGVLSASNVLISETGELLRS
                     TNVKDGYILRYAIKKGLRVIILSGGTNESVKIRYEALGIPDVIIKSKRKIDDFAKLKE
                     KYNLDEGKTLYMGDDIPDYELLKTVGLACCPADAADEIKEISLYVSSKKSGEGCVRDI
                     TEQVLRAKGLWMDDDAFNT"
     gene            45863..46837
                     /locus_tag="PKN53_00455"
     CDS             45863..46837
                     /locus_tag="PKN53_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOD87457.1"
                     /translation="MNTNILKLIRWKNLFIIVLTMIAMRYAIAQALFAYYKIPPAFPD
                     IGFVFLVLSAVFLVMAGNFINDYFDRKTDFINKPKKTLVAFKVRRRQLISLNVVFNVL
                     GLICGFIAAYFAHKLTLGIMFIFIVAVLWTYSAELKRILFIGNFSLALIIALIPLSVA
                     LTEYFALEHSITQWEENSIRAIKLSVQFIIGFSIFAFLFTYIRELIKDCIEYNGDLET
                     GIKSFPTVFGKRKTDIIIAILSLVAIIAIILCWEVYLSKLLFLRNHIIAPFYIYAFLV
                     FPTALLFLIALTLKTPKKHKTMLTLINLIIVFGILFSFIFSSIVYGKI"
     gene            46824..47411
                     /locus_tag="PKN53_00460"
     CDS             46824..47411
                     /locus_tag="PKN53_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HOD87458.1"
                     /translation="MEKFNFVKPNYTILLASGSPRRQELISKFNFNLKIVKAPENKEI
                     IPDNVFRENIAIHLAKEKSLSYKDLKENEILVTADTIVWKDNKILGKPKDKEEAIEML
                     EFLSGDEHFVFTGVCLRSINKTKSFFAETKVKFSKLSKAEILYYIENYKPYDKAGAYG
                     IQEWIGMIAVEYIEGSFYNVMGLPVQKLYKEIMEF"
     gene            47957..50113
                     /locus_tag="PKN53_00465"
     CDS             47957..50113
                     /locus_tag="PKN53_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOD87459.1"
                     /translation="MKKLIILILSSILFFPALADEGMWIPILIERYNFDDMKAKGFKL
                     SAEDIYSINKASMKDAIVIFGGGCTGELISDQGLLITNHHCGYGQIQQHSSLENDYLT
                     NGFWAMSQKDELANPGLKVTFLISMSEVTDAVLEGVNDNMSEQERYSIIKKNIDKIKK
                     ASVEGSHYEARVEQFYNNNQYFLFINEIFTDVRLVGAPPSAIGKFGGDTDNWMWPRHT
                     GDFALFRIYCDKDGKPAPYSEDNVPYKPKKFFPISTKGYKQGDFTMVFGYPGTTTEYL
                     PSSAVEQVMNETDPSRIMIRKKKLDIINAAMNSDPLIRIQYSAKAASIANGWKKWIGE
                     INGLKRLDAVNVKKQYEQDFMNWVNADSERTKKYKYILSQYKTIYENLSPYNLAMIYI
                     NEAGFGSDAVLLALRMRSLEGVVGKSAESIEVLKKDAQTRADNHFKNYNEETDKKMFA
                     EMLKLYYENIPQEFHPNYFAQLDKIKVKTGNKYEAIAEHCYKTSIFGNQEKFNNFLNS
                     FSEKSYKTLMKDPIYLLMKDYVDIYINKVDAEFSRYSTQLDSLDRIYMKAQIEHQDDK
                     VLFPDANFTLRITYGKIDSYYPADGVKYNYFTTLEGIMEKDNPEVYDYDVPQKLRDLY
                     EAKDYGRYADADGSLHICFTASNHTTGGNSGSPVINGNGELIGINFDRNWEGTMSDIM
                     YDPEMCRNISIDIRYALFIIDKYAGATHLLKEMDIR"
     gene            50116..50763
                     /locus_tag="PKN53_00470"
     CDS             50116..50763
                     /locus_tag="PKN53_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87460.1"
                     /translation="MKVGFVSILLLIFATFGNVNSQNLYYHFYDDVQVFTDSTSLLNA
                     IFLDDVYKHVYKNDSISFWIIEADTCDASDVSGIPYCQSCSVGANLILQNSYKFSIID
                     EKLVVGTCWDYLNAIYRSVESSDLFRKTQVFNSKKSGPYAPKEMLKPGDWVYHINRQF
                     RNIEHSAIFVAWKDYEKGIAITMSYMGLGRARPARYGEYDLNSVYAIFRIEDEFW"
     gene            complement(50777..51496)
                     /locus_tag="PKN53_00475"
     CDS             complement(50777..51496)
                     /locus_tag="PKN53_00475"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOD87461.1"
                     /translation="MLPKIKRSKYILILLLVLSSYSLKAWEVRTDSTLFVNPWVGLSH
                     YKILGFPQSFSSNYLSYGIDFDYRFHSELSFKFGVSLMSKGNYYFASYVNAENTPMGE
                     FKTFNKFQYLSFPLLLTYNLFGGKKLNLFIDLGVNAEFLLQAKTYAKLPKTFEGVSVE
                     QVNVVNTDTYKPLNFSLVVGCGFEYKIKPNLLVFIQAKYDHGINQILKENSTYIFKHR
                     GYCAGIGIKIGIPIKYTTSTN"
     gene            complement(51471..52850)
                     /gene="xseA"
                     /locus_tag="PKN53_00480"
     CDS             complement(51471..52850)
                     /gene="xseA"
                     /locus_tag="PKN53_00480"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOD87462.1"
                     /translation="MSRQSLTLLELNEKIKSAINESFDAPVWLVAEINNINLHRSGHC
                     YLELVQKSEYDDKIIAQMRATIWASQYRFIAAYFKSVTQRDIEKGMSILVKVSVEFHE
                     LYSISLNIREIEPSFTLGDLEKRKKEIIDKLISEGVFDMNKLLHLPDVIQRVAIISSS
                     SAAGFEDFINHIENNKFGYKFELSLFEANMQGTGTEESVIEALNEIYENIENFDVVAI
                     IRGGGSKSDLSYFDNYNIAYHVTQFPIPVIAGIGHERDESVTDMVAHTKLKTPTAVAN
                     FIVDYNNVFETEVFEIFKSIVTETQYVIKNKEMYLASLGLKIYKIKDVLIKNNEDCNR
                     NMYRVKNASVQLIRKNENRLNNYFENLKLLTHQKTYLANKHLENSFSSLKKNTEILLS
                     KNELKLQNLSKNIELANPENVLKRGFSISKINGKIIDENTNIMVGDELETITFSNKIL
                     SNVTKNKKK"
     gene            complement(52852..54510)
                     /locus_tag="PKN53_00485"
     CDS             complement(52852..54510)
                     /locus_tag="PKN53_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOD87463.1"
                     /translation="MKIKFLVFFTFLSLNIFAQLEVAPNKYYFELTDKNHNLYSLDSP
                     EEFLSFRALQRRANQNIMINYSDLPVSQFYIDSIRNCGVEILNVSKWFNSVLVYSENP
                     EPIELIKNLSFVSGSITYEIKTEDERVLGNEYKVLRDIQKGEEGDFYYKYGPSQNQIF
                     MHNGQKLHNKGYRGQGMLIAVIDAGFTATLDVPSLQHVFQDNRVIATRNFVHGGDSVF
                     NYSTHGMRVFSILAGNSPYYLVGSAPEAEYVLLMSEDPITENLIEEINWISAAEFADS
                     IGTDIVNVSLGYLTFDMPVNNHSYEDMDGETTIIALAAREASHKGMLLVVSAANSGES
                     QSHPWIASPGDAKDIITAGAVYADSTYAAFSSIGPSYDGRIKPDLVAMGAGTFNQEPN
                     GGFGYGNGTSFSAPLLAGMFACLWQQFPEKTNFEIMEIAKQTANLYNNPNAYLGYGIP
                     DFDLASQILTVEKEISKMEKLTLSPNPYYHDFNIHLDNNIGDEIVIEVSDLLCRKHYL
                     WFKLRASENNQTVNIPDLANLKPSIYIVTVSINSKKYSAKLIKQ"
     gene            complement(54532..55224)
                     /locus_tag="PKN53_00490"
     CDS             complement(54532..55224)
                     /locus_tag="PKN53_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87464.1"
                     /translation="MKTRIFILLILSLGLVACNKENIKPRKFPLGQEYFPLIEGNSII
                     YKVTEINIDKPSNVYDTSIYYLNEIVESPFIDNENDTAWRIERYTRKDENYSWIINSV
                     WSAKLTNKYAVKVEENQRKIKIRFPIKENLSWNGNLLNNQKVQEYEILRFDHKFNIGS
                     VQFDSCLTVMQDSSVSLIHKDLAYEIYAKNIGLVYKKEIYINSQEVTHNVPIEERITV
                     GRIFIQEFVRED"
     gene            complement(55237..56313)
                     /gene="mnmA"
                     /locus_tag="PKN53_00495"
     CDS             complement(55237..56313)
                     /gene="mnmA"
                     /locus_tag="PKN53_00495"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOD87465.1"
                     /translation="MATKKKILLAMSGGTDSSVVALLLKEQGFDVQGVTFHAYDNILK
                     SCADNFDSFVEAENLAKKLGFPHQILDIREYFDKIVITNFISEYMAGFTPNPCVLCNK
                     TVKWGKMLDLMKNLNCEYYATGHYAQIVNENGRYFLRKAKDTNKDQTYFLWTLSQENL
                     AQTIFPLGSLLKSEVREIAHKHQCEKIANKKESQEICFIPDNDYRQFLRERIPEINDN
                     PGQGNFIDTNGNILGKHKGFPFYTIGQRKGLEIAMGYPVYVTNIDAKTNTITLGKREE
                     LLQTTVWIKDVNLMKYEEIKGEMQGNCKIRYNNEGEDCSFIMEGEMIKINFKQPVSAV
                     TPGQSAVIYENNDLVCGGIIAKIN"
     gene            complement(56368..58338)
                     /locus_tag="PKN53_00500"
     CDS             complement(56368..58338)
                     /locus_tag="PKN53_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOD87466.1"
                     /translation="MRQILVILIFCVISVLSYSQVDVKFTPANFPNKKEQLKVARSQI
                     KNADIYVYSENKIEEGLKLYLKANEFNPNNSDLNFKIGYCYYNSSQKYDCLEYFKKAY
                     ELNPKVDKKIEFYLARGYHFNYDFDKAISLYSSYLQDVKDKVELEAVGKHIAECRNGK
                     ELVKDTVKVEIINLGENINTEYREYGPMVVADGSKLYFTSRRKGSTGGQVAPDGMFFE
                     DIYESNKTEDGWTKARSVGSPLNTKLHDAVVGISPDGQTMYIYMDTNGGDIYETKLKG
                     SRWSKPTPLKGNVNTKYHESMAGISYDNKTLYFISDNPATSIGGRDIFYATLDKNGNW
                     SSPVNLGPVVNTPYDEVDFFLHPDGRTMYFCSKGHNSMGGLDIFKTVKNADGEWTTPV
                     NLGYPINTPLDDAFYVTTASGTTAYYASVRPEGYGLLDLYELKYHVDKETQKEEDKVL
                     VTLVKGVVRDATTTFPLEAKIEIIDNSIDEVIAEFITNSQTGGYLINLPSGKNYGLNV
                     SKEGYLFHSENFNIADTAEYNEAVIDVNLQRIEVGITVVLKNIFFDYDRATLRPESFA
                     ELNRVLAILNDQPKLKIEISGHTDDRGSLEYNKKLSRERAQSVVDYLIEKGVDKNRLT
                     YEGYAFEKPIATNETDEGRQMNRRVEFKVIAH"
     gene            complement(58349..60451)
                     /locus_tag="PKN53_00505"
     CDS             complement(58349..60451)
                     /locus_tag="PKN53_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOD87467.1"
                     /translation="MKTSKFLIIFSILFAIGVQTYSQVEVEINKKEFKQSKDGYKEAM
                     KALKVAEYYYSLGQSGAYRAALPEYLKANQYNPHSPELNYKIGACYLYSTEKTRALPF
                     LEKAYKAKPNVAVDVCYLLGMAYQYNYDFDQALTSYRSFKHEYSGVVSKKSKKKDGPE
                     YDVDKRIEECESGKKLIKNPIRVFIDNLGLAINSKYPDYGPVITADQSVLIFTSRREG
                     STGGELDPNDMIYYEDLYVSYNDEKGWSQAVNMRDLNTDGHDATVGLSPDGQTLYTYK
                     GVPDGTIFESKLRGNTWSKPKELNKNINTKYHETSASISYDGKRLYFVSDKPKDDFGN
                     SSYGGKDIYVSELQPNGNWGPSKNIGAPINSKYDEEGVFSHPDGRTLYFSSKREGGMG
                     GYDIYYSEKDDMGKWTEPVNIGYPVNTPEDDVFFTVAGNARYAYYSSSREGGYGLQDI
                     YKITFLGPEKMMVMGAEDILIASSNATISQKVKQEVVEVKKVRLTIMKGTITDAISTA
                     PVEASIEIVDNEKNELISTISSNAATGKYLVSLPSGKDYGIAVKAQDYLFYSENINIP
                     DATDYQEVTKDIVLSKVGIGSKIVLKNIFFDYGKETLRPTSLPELDRLVALLNSYPSM
                     TIEIGGHTDNHGSLKFNTDLSEARAKSVVDYLVDKGISKSRLTYKGYAYLQPIATNDT
                     EEGRQENRRVEFKVISIK"
     gene            complement(60479..61600)
                     /locus_tag="PKN53_00510"
     CDS             complement(60479..61600)
                     /locus_tag="PKN53_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD87468.1"
                     /translation="MKKQILIFVISIIPFFALAQEIDTSQIIIPIKIGYDSDRTAISD
                     TTSTLVLADDQEANVAFNDAVQLYQQGQYQAAIQSYSKALQMNPNLIDAYKGRGSSYV
                     QLSNFKSAESDFEKFFAKKQNSGEAAYLLAYSQYYLDKLEDAEKNFLKAKSLNHKDVN
                     LYYFLGVIKFNQEKYSEAIEFYNEAIKIDAKHVFSYNDRASANRMLENYDAAISDYSK
                     AIELDAYNPLLYNNLGSVKRLNKDYAGAIEAFDHALRIDRDYFLAINNKGITLYDQGK
                     YQEAIECFNNVLKLKKDYVYAYNNMGLCYYKDKDYKSAIEAYNKAIFFDPSYAYAYIH
                     RGSAYEMLRQMEDACADWKKARDLGLEVAKEYCSVCKEY"
     gene            complement(61672..64950)
                     /gene="secA"
                     /locus_tag="PKN53_00515"
     CDS             complement(61672..64950)
                     /gene="secA"
                     /locus_tag="PKN53_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOD87469.1"
                     /translation="MGFGSFILKLFGSKSEKDLKAIQPFLDKVLAIEPEITKDSNDEL
                     RARSENLKKKIAEHIKPEQDKITELKAKAESLDIWEREEIFNEIDKIEEKIDKDLESY
                     LNEILPEAFAIVKETARRFKENKEVVVTASEFDKNLATHNDFVEIKGDKAVWFNSWVA
                     GGNTVTWDMVHYDVQIIGGVVLHSGKIAEMATGEGKTLVATLPVFLNALTGRGVHVVT
                     VNDYLAKRDSEWMGPIYMFHGLSVDCIDKHEPNSEARRIAYMADITFGTNNEFGFDYL
                     RDNMALNPSDLVQRRHNYAIVDEVDSVLIDDARTPLIISGPTPKGEDQLFDELKPHVQ
                     KLVNAQRDLFNEIFNEAKKLIAADNEKDGGFKLLQAFKALPKHKPLIKFLSEQGMKTL
                     LQKTENFYMADNNRQMHLVTEDLYFVIDEKQNSIELTDKGFDLLAHATSDPNFFVLPD
                     IATELSELSKSTLPENERLNKKDAIISDFSIKSERIHTVSQLLKAYTLFEKDVEYVVI
                     DNKVKIVDEQTGRIMEGRRYSDGLHQAIEAKENVKIEAATQTYATITLQNYFRMYHKL
                     SGMTGTAETEAGEFWDIYKLDVVVIPTNRPIIRIDREDLVYKTNREKYGAVINEIERI
                     IKEGRPVLVGTTSVEISELLSKILTRKGIKHNVLNAKLHKREADIVAEAGYSSNVTIA
                     TNMAGRGTDIKLSDKVKEAGGLAIIGTERHESRRVDRQLRGRAGRQGDPGSSQFFVSL
                     EDDLMRLFGSERIARMMDRMGLKEGEVIQHSMISKSIERAQKKVEENNFGIRKRLLEY
                     DDVMNSQRTVIYKRRRNALDGERLGADIANMIYDVCVSLADEYFENYEYEEVKLELFR
                     KLSIELDLSEQEYENLKYDDFIDYIYGKARENHKRKAQAIADQAYPIIKTVFENKQSI
                     YNNILVPISDGKKEYPVIVNLEKTYNNKGEEIVNAYDKTIVLKTIDEYWKEHLREMDD
                     LRQSVQSASYEQKDPLLIYKLESFELFKAMLDKVNKSVVYSLMRGFIPVQEADEVREA
                     RVRSKLDMSKMKTGREDPGYHDPSKGKSVADPVRVEKKVGRNEPCPCGSGKKYKHCHG
                     INAD"
     gene            complement(65066..66154)
                     /locus_tag="PKN53_00520"
     CDS             complement(65066..66154)
                     /locus_tag="PKN53_00520"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOD87470.1"
                     /translation="MNNFFVKIRVFLVVLLLFLFGKSYSQIEFQSLFNTNLKEGVSCY
                     RIPALAKAPNGDLIAAIDERVVSCGDLRYNEDINIAIRRSEDNGKTWSEIEVIVDFPL
                     GESASDPSIIVDKITGEIFLFYNYMNLKLEKEVYYLHYVKSADNGKTWSKHVDITEQI
                     SKPEWHNNFKFITSGEGIQTNSGKLLHTLVNLENGLCVFGSDNHGETWYFIDNAIKPA
                     DESKIIELADGTLMINSRVNGLGYRYVHLSDDYGKTWTSKPDSALIDPSCNSSILNYS
                     YEIEGESKSILIFSNLESKNKRENLSVKYSLDNGATWSKSKTIYAGSAAYSSLCVLQN
                     GDIGLFFEKDNYTDNVFTSFSLNWLLAE"
     gene            complement(66147..66353)
                     /gene="xseB"
                     /locus_tag="PKN53_00525"
     CDS             complement(66147..66353)
                     /gene="xseB"
                     /locus_tag="PKN53_00525"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOD87471.1"
                     /translation="MSEKEFSYTKAMNELEEILAEIETDELDLDVLSKKVKRATFLIK
                     ECKSRLRKTTDEINGILSDWEKDE"
     gene            complement(66372..67901)
                     /gene="guaA"
                     /locus_tag="PKN53_00530"
     CDS             complement(66372..67901)
                     /gene="guaA"
                     /locus_tag="PKN53_00530"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOD87472.1"
                     /translation="MAEKILILDFGSQYTQLIARRLRDLSVYCEIYPYNKIPELDSTI
                     KGVILSGSPYSVRDKDALIPDLKNIKGKLPLLGICYGAQYLSHFYGGEVAYSNTREYG
                     RVNLKFVDSSDKLFKGVDQHSQVWMSHGDTIVKVPDNYKIIASTNDVKVAAYKIADEA
                     TWGIQFHAETSHSLEGNKILENFVVDICGCKKDWTSASFIDETISELKQKIGKDKVVL
                     GLSGGVDSTVTGVLLNKAIGSQLTCVFVDNGLLRKNEFEKVLESYKGMGLNVIGVDAK
                     QRFYKELAGVKDPEQKRKIIGKLFIEVFDEEAHKIEDVVWLGQGTIYPDVIESVSVKG
                     PSVTIKSHHNVGGLPDYMKLKIVEPLRLLFKDEVRIVGKDLGISDSLLGRHPFPGPGL
                     AIRILGEVTEEKVKIIQNVDDIFISSLKEWGLYDKVWQAGAILLPVQSVGVMGDERTY
                     ENVVALRSVNSIDGMTADWVHLPYEFLAKVSNDIINKVRGVNRVVYDISSKPPATIEW
                     E"
     gene            68000..69094
                     /locus_tag="PKN53_00535"
     CDS             68000..69094
                     /locus_tag="PKN53_00535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOD87473.1"
                     /translation="MNLKDIKYDCRHFDGAIPCVPNKKHGVDCLNCKYYDKIGKKILI
                     IKLGAMGDVIRTTPLVVKYRELYPDCHITWLTLSPEVLPKDKIDKILKWNESSIFIIQ
                     NKKYDIAINLDKEDEACILLQKVEAKEKYGFIWKDNHIDAATQAAEHKLITGFFDHYS
                     QKNTKHYLDEIFEICHLQFNDEPYLLNYNKNLAEQWQAKIKEKSKGKTIVGLNTGVGN
                     RWATRLLPEEHWISLINLLLKNDFYPIILGGPQENEKNSRFADKTGAYYPGTFSLEEF
                     FAISSACEIIVTQVSMMMHIATALQKKMVLMNNIFNKHEFYLYNRGVIVEPESGCDCF
                     YGNKCKRETSCMYDVKPETLLKEILKLKKI"
     gene            69094..>70034
                     /locus_tag="PKN53_00540"
     CDS             69094..>70034
                     /locus_tag="PKN53_00540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD87474.1"
                     /translation="MRIAYLSTFYPFRGGIAQFNASLFREFEKAGHTTQAFTFTTQYP
                     QFLFPGETQFVQENDIVDKIDSQRILSTANPFTWCSAAKKIRKTNPDLLIMKYWMSYF
                     GPSLGFVAGKMNKDTKVLTILDNVIPHEKKFFDSAFTRYFLKKNHAFIAMSEKVKNDL
                     LTFLPEAKCELLPHPLYNHFGDIQDKTKAKETLGIEPNKKTLLFFGFIRDYKGLDILI
                     ESFGELDKSYQLLIAGEVYGNFSKYDELINKNPNKENIHKHIKYISDNEVSTYFSASD
                     ICVLPYKSATQSGITGISFHFETPMIATDVGGLAEIIS"
BASE COUNT        24022 a        11715 c        10040 g        24257 t
ORIGIN      
        1 actgcatcat ttacaatttt ttgatcattt ccatatccat ttgttacagt aagagaaact
       61 gtatatgtac ctgaactagc ataactatga gttggatttt gtgcaacact agtatttcca
      121 tcaccaaaac tccaaagcca agaatctgct aagcttgaca tatctgtaaa gctaacttct
      181 cctgaacaaa atgcaggtcc acttatggca aaatcagcaa ctggaggaat atttggactt
      241 attaaattaa atgtataatc ttcaacttct ccatagccag aatttccaca aggagttgca
      301 tttggattat agctagagcc tgtgtcatgt aaacgtattc ttactctcac ttctccctga
      361 gttacagtac ttggaactgt aatatttcca gaaaatggtc cttgtccatt tccagaaaaa
      421 acattttctc catcatcaat aaaatcgcca tctctattcc aatcaaccca aatcaaaact
      481 tggtcactag tataaccatt tgcagtggta atagaaaacg gataagattg tcctatcaac
      541 aaatctgttg atatatgtgt gaaatctgta taagttgatt ttcctgaaga attatttatt
      601 gtattacaag taaaattaga aattccttca tagctagcac tattagaacc tgctgagcaa
      661 taagttggaa gagtaacagt aatataagct aatttttctt caacatcact tccatatgta
      721 tttgaagctg ttaatttaac tttataagtt cctggtgtat tataaactac actaggattt
      781 tgttcggtac ttgtagcagg tgtaccacct tcaaattccc aagaccaacc tgtaggattg
      841 ttaatagaca aatctgtaaa accaactgta gtacctataa ttatattagt ttgactagca
      901 gtaaaatctg ctgttggtgg gtttgtattt atatttacag taatataatt attttttgtt
      961 tctgtatctg agccaagagt attagtaaca gttagactta cagtataagt tccagctgca
     1021 gtatatacat gactaggatt ttgttctgtt gaagtttgtc catctccaaa attccaagac
     1081 caactagctg catattgaga agcatctgta aagctcacag tttcgccttc taatattgta
     1141 gttggacttg cactaaagtt agctactgga acactaggaa ctgttggtaa gacagactca
     1201 tttatatatg gagctctatc ttgttttgtt cctactacac aatggttttc gcctgtaagt
     1261 gcggggaaat ttaatgttac agtacttcca ctacccgacc acttagcatc taacaaaaca
     1321 ttattctttg agattgcaac ataagtataa ggttctgtat taaccgttaa tgtagttgct
     1381 ccaacaaaag gaggtgtaaa ggtgagtgtt agtggatctg gctttgtcat ataaggtatt
     1441 agagaaggat cgccagaaac gtgataaatt tcccaataat attgttttcg actagtttct
     1501 gaactagctt gtactgctaa atttccacaa gtaataattt gcgaagctgt ataataccaa
     1561 tttgcatggt catctccata gtgttcatga aaaactccgt ccataaaccc taagcctgta
     1621 ttagaagtat tataagcagt aatatttcca gcatcaactg ttgcctgaga aactaaacca
     1681 agtccccaat aagtatcttc gtaccaataa gaatagtttg tggttccaat ataacctact
     1741 gctcctgcat ctttagtatg taaaagcact tctccaaaag catcactttt attaaattga
     1801 aaagacaaac aacagtttcc tattaaaaat ggatatttat ttttatttgc ccaatctgga
     1861 acattagatc taagaactgc agggtctgcc cagccatcat ggtcacaatg agcagtataa
     1921 tttccaaaac caacaccagc tccaattttt gaatttatat caccagaagc acttgaagag
     1981 tttgatctca taaccgaata aggatatgaa gtcgtattgt aataatatgc ataaatattt
     2041 gcaaaaccat gagctgtatt tatataatta tttgttgcat ataaaatagc tgcatttccg
     2101 catttatttg cccaataagg atccactcca gcaactaaaa gagctttatc caaataatca
     2161 aaatttgaaa gtgaatattg ttgataaggt aaaatcttat ctaaagcatt ttgaagctct
     2221 gttgttgatt ctgctgaaat tctaccataa tacatatctg gaagataatc gcttgtccca
     2281 tcaaaacatg caaaataaag gtcagtgata tgactttcgc cagaaacctt agttgcaaat
     2341 gcaggaatag aataagtacc actatggtcg ccaattatca aacaataaat tggagctggg
     2401 tctgaagcag ttcctgcatc atataaacct tgtaaataat ttttaattgt tgtatttgta
     2461 gttccactag caacataatg ttcaatcaca ttaaaacctt gtttagtttt ccattgcaca
     2521 aatggttgca aagttgtttg aaaagcttgg tttgcaacaa taacatattt taatggagct
     2581 gaaaaatttg taagctgatc ttttgctgct ggtgccgaaa aattaataag attagaataa
     2641 gctcctgcaa attctggaga ataatatctg ttcttcattt cttcggttaa agctaaatca
     2701 gcatttacaa acttgatttc aaaatctaaa tcattatata ctatcaaagt gttttctact
     2761 ggattataat gataaggacg aatagtaaga cctgctattc taactcctct catagttcca
     2821 tagatttcaa ccttaacaat atcttcagta tcataaagat ctgtattgta ataattttca
     2881 tctattacaa aatattgctc ctcaattggt gtactcttgc tatacgaagg ctgaactggc
     2941 tctatcctat taatcccata ttccgaaaga tttataactt cttgggaata accatgaatt
     3001 aaaacctgaa cttcggcacc atgaggaact tcaatcattc tactcaaagt tggaactcca
     3061 gcttgtccaa cagtgcctgt ggaagcaaag ccttctaaat cgatagaagc aaattcgcga
     3121 ttttcgatta ctttatttgt cgcttttagt tctttcatct gaacttttgc agagaaagct
     3181 gtcggtgtaa atcctttaaa ttctaattta ttatgattta cagacaatgg aattatttcg
     3241 ctttgttgag aaaacaaaaa caaactaaaa ccaagaaata cgatgaggaa aattagtttt
     3301 ttcataatat ttaaagatat agttagacac aaaaaataat ttctttaaaa ataatcaatt
     3361 tttaatttaa ttacaaataa ttaaattatt ttatatgaac atatttttta cacaaaaaaa
     3421 actgcaataa aaacatactg tttgtaaaat ttgtaaaaaa agcactattt atatttatgt
     3481 tctcctaatt ttaaatcaca actaacctca taaacataat gacaaggttt atcacaacca
     3541 gttttaaaga atttagtttt tactaaagca tcatttatca cataaatagt tttatcaccg
     3601 tcaaaatatt cagcaactac agtatatttt tggtttggtt ttacctgtat ccacaattca
     3661 ttaacatctg tattcccctc catccaaaca gctgatgtaa aagcataacc agaaaacaca
     3721 ataaattcta ctttttcttt atattttttc atattaaaaa ctaatttcaa atcaaccaat
     3781 tctggcatat cctcgtagca atactgacag tcaatcgttt cgctatcaac agaagtaata
     3841 taatcatcat cagcacacgc ttttaaactt agtaaagcta taaagctagc actaataatt
     3901 aataatgtat ttatgattga taagactttc attttttcta aaatttacta aaaggctcaa
     3961 actaagcgaa taaaaaaact tagattgtgc atatatattt gttgttaaat attctgtaga
     4021 aattttaaac ttacaattag agcgattatg aaggctaatt cctataaatg gcgaagcaat
     4081 aattccgtca attattttaa attctactcc atcgtatttc ccccaccatt ttgttgctct
     4141 caaacctgca taaaagtcca tataaaaatt atttttgctt ctaagcaagt taaaattctt
     4201 taaacctata agatatattc cttttcttga ttcccgcaac tgatagtaag agtgattatt
     4261 aagaataggt agtaaaacta atttttcaaa atgtctataa aaaaaacctg tttcaaattc
     4321 taaaccacat ctagcatcta aaacacctag tcctgttgtc caaaaaaaat catttttgct
     4381 aaaaatcatt ccttggctaa gtgctaacct atcaaaccac aaataattat aatctttaat
     4441 attaagtttt ttaataattt tttttatgtt tctatcgcca ccatatttgg ctaaagtttt
     4501 gggtgtataa gctgaattaa ttgtatctct aagttcataa ttattttcca aaaaccattt
     4561 taacatttcg tattgtccat aaataattga agattcaatt ggtgttaatc catatatatt
     4621 ttcttttaaa tcaatttcgt ttctaaaatt ttcaaaatac caaatattat tattttcagc
     4681 tgctaaatga aaaacattgg agctatccga aagcacgaaa tcaacgtctg catcatgtaa
     4741 acgcaataac ataactgcac tattgttatt atacattaca gcgtacatca atggcgtaaa
     4801 gccgtaatca tctttcaagt ctgcttctgc ttcatttgat agcaacaaat cgagaatagc
     4861 atcatacccg taaatacttg cataatgtac acaagttcgt tcatcaaaat cttttgtatt
     4921 aatatctgct ttagcattta gtaaaatttc aacaattttt aaatgattaa atttcacagc
     4981 actatgtaaa gcggtatttc cattttcgaa agaagctaaa ttcaaatctg ctttattctg
     5041 aaccaaaaac tccactactg aagtatgtcc ttgttctgcc gcaaacatta aagcagaaac
     5101 gccataataa tcaacaaagt tcacactagt tttacctcct tcgagtattt cttttacctc
     5161 ttctaaatct ccattataag cacaatacaa aagtctatca ccataataat aatattcatc
     5221 tgtatagtat tcttctgatt gagaaaacaa attcagaaat aatatagaaa gtaaatatgc
     5281 tagcaaatac tttttcatgc aacaaattta aaaaaatatc taatacttta attaaataag
     5341 ctgaattaaa tttagtattt ttgcaacatg gtcccttagt tcaatggata gaatgtaaga
     5401 ttccggttct taagatagag gttcgactcc tctagggact actgatatca gttaaaagtt
     5461 gaaagcgatg cactgaacgt agttgaagtg ttgaaccgag tttacgagct caccgaaggt
     5521 aaagaggctg acgcgtcgat tgcttcgcag agttcgcaag ctcactctac tcgcatgggt
     5581 ttgttttaaa ccacaaaaga cacaaaagga cacaaaagta aaaagttgaa agtactttaa
     5641 aaaaaagagg ctgcttcaaa aataaaacag cctctttttt tattgttatt ctcaaacttt
     5701 taatattccc ataggtcgtg ttctattaaa gcaatttttt cttgtattct ttcgccttct
     5761 ctaattgcat caattcctaa caaatagtct gaaatcaaac gattatcata attatttgat
     5821 tctgctacaa tatagctaga gaattttctt ttgaagaaaa tatcatcaaa agttctatgt
     5881 tctgcatcgt tgtgcgaatt aaacacttca tatcttgcaa atagtggtct ataatcaggg
     5941 aaatatatcc agaatggcca tactctttca acggtttcgt ctttaacaac ccatccgtct
     6001 ggagcaagtg ctataattct cacatccatt aaagaacgtt gtttatcaaa aacccattct
     6061 tcccaaaccc acaatttatc taaaacacga gtatcgtcaa gtttggagtc gatagtttct
     6121 tcataagttc ctccttcacc atcaggcaca agaatttttt ccttataagc caataaatgt
     6181 tctctaagtt cgtcatcttt ttctattgga tgtctccacc aattagtaat atctgcagca
     6241 tctaatttgt acactggata tcttcttact gtatcaatca atgttaattc gccggtaata
     6301 tcactatatt gttcgccaag aatacctaca ttatacatca aataaatcaa gttaactcta
     6361 cttctttgag gttgtatttt cactggtcgt ccttcattat ctaatcctgt tccaggataa
     6421 gtcattctaa caattgggaa atacaacgga tggttttgtt tttcccttaa ttctaatact
     6481 cttagaattt ttttagagta catagcatct gcttctctaa tattttgata aggaacgaaa
     6541 gtgtgttctg ttacatgttc tctcacataa gctccatcaa gaacttgtgc acttattggc
     6601 aaacataggc tgataagtac aatgagaggg aaaaacttat taactatttt cataatatta
     6661 aatttttaaa ttattgtatt ttcacagtta ttccattagt ctttctttca cctgaagggc
     6721 tactaactct aatatttgta aagcttgctg ctgatccagg atttaaatta ttaagaattg
     6781 tttgtggaat atttgaacct gttccagcaa ttgtttgcgt tacacctctt acagaatata
     6841 agaactcata agaaataaca ctatagctta ctcctttaaa tgggaagaag tcagattcta
     6901 ggttagctgt taaagtaggt gttctagtaa tagctccttt aggaacagaa gcaccgtctt
     6961 tgtaagctcc tgcaataact attgttggag gtggtacgtt taacacagag aaagtcttag
     7021 aacctaaagt acgtacgcct tcttctgtcc taacagaaac agtaatactt acctctcttg
     7081 ctgatactga agctggaact tttactatat aaccagcacc agatctagat atagaagcac
     7141 cgccagaaat actaacactt accgcttctt gtggataccc agatactgca acttctacgg
     7201 ggttatcaag ccctctataa aatacattac atgcacttgc ggatacggta aagcctgcat
     7261 caccaacttg ataaacagct ttataaggca accacatatc acctttatta gatttataat
     7321 gcactaaacc accatagttg aaatcgccta ctccgccaca atcacgaaca tgcaaacctt
     7381 ttccagactc atcaataggt aaagtatcgt aatttgctcc ttgtctaagt ttatactgaa
     7441 cctcaccatt tactactgtt gagtcataaa atggagcatt ataagtaaga tatattgttg
     7501 gtctcataga agtatcacga gctcctagga acacgttaga agaatattta cctccgcgaa
     7561 taacaaaact acgtggtgaa cttactaaag cttcaagagc ggtaatacga atatctaatc
     7621 cttctagttc agctatcata aattctaaca catcagcttc tgaatttcta atatcagctt
     7681 gtaatttaga taacattgcc attgtaccaa tcataggcat accacgacat aaagtttctt
     7741 cccaagacca aggtgcttct ttattatcaa attggtcttt agcttccaaa tttttcgcta
     7801 cgtttttata aactgttaaa gaagtatctt caattattcc taataaaagt tctcgataac
     7861 tatctaacca acctctaagt tgttttccca tcataggacc accttctcca accatcacaa
     7921 tagttgcagc gtgcaagtca tccatctttt taatttcacc gtcaagtgtt gcctcaggac
     7981 cttgactttc acgcaatatt aacaatttta ggtcttcaat attttgaata aattcatttg
     8041 attgtcgctc aacctcatga gcttgttggg ctaatttagt atatttacct ggttgttcta
     8101 aagcactatt atcgattttc gcgtataaag acttagtttt ttctgaataa ttagaagttg
     8161 tcttttgaat actttcgttt attttaacaa aagaagttaa aatttcagaa gatacgttca
     8221 aagcgagcaa tgctgtataa aacaggtaca tcatcccaat cattttttgc cgcggggtct
     8281 ccggacaatt tgttgctgcc atatattttt aaattttgaa attcaacata aaattaacaa
     8341 cctctaatta atctgcttta aaattcattg ctgaaagcat attaccataa atggtattca
     8401 ttgcagccaa attatgactt aatttactta tttcttcacg atattttctt gtatcttcag
     8461 cagattgttt catattatct aaaatctcat ctaagccagt gtacatagct ttagttgctt
     8521 caatttgttg gttagttcct tgtaattgaa gttcatacac tgcatttaat gctgtaagat
     8581 ttttattcat tatacttagt tgttcattat aagttttacc tccgctagtt gacatttcca
     8641 actctttatt catattttca atcaacttat tataggtact tgctaattgt tcgcctgaat
     8701 caccaactac agaagcagat ttttcataag tacgagcaag tttatcagct tcaactttta
     8761 atctttctgc cgaacttcca taagcatctg caaaactaga aactttttca gacgctttct
     8821 caaaattagc aacataatta tttgttgcaa ctgttgcgtc agaaacatct tgaagttttt
     8881 ctgctgtgtt gttcaaattt tgaagtccat gtcctaattt ttcaaaaagt tctggtgtta
     8941 tttctgcatt ttcaatcatt gcatcaaatt tttctaatgc agattttttt gaagaaatag
     9001 cagctttttt atcaagcaca tttaaatttt cttctatacc atctaattct ggatagacta
     9061 gtccccaatc atattctaca tgaggtggct caaaagcaga aaggaagaaa atcaacgctt
     9121 cggttaaaag accaaccgtt aacattatgg tagctccagg taagtgttca attttaaata
     9181 atgcaccaac cataacgatt gcagcccccc aaccgtaaat gaaacccata aatttcttcc
     9241 atcccttact tacaaaaagt tcactaaaac tcataattgt gtaattttta attaatactc
     9301 tatttattta atttatattt atatttaaac ttttcaattt tcacacctat atattaatat
     9361 acttgagaat aagaagttcc gtcacctcta tgtcgtccta aataaggtct tacacaacgg
     9421 aagccaatat agctctttgc agaatcttga tattcataag tacgtgttcc attttgcata
     9481 taataagcaa catctttcca tgagccacca cgaactactt tacgtttttt tgctggtggg
     9541 tcgtctggat gagcataata ttgataatca ggattcaaat cgtgagtaaa actataagca
     9601 ctttcgtcat aagcattgct tgtccattct gctacatttc cagccatatc atacaagccc
     9661 cactcgtttg gatcgtaagt agcaaccatt aaagtttgta ctccaccatc gtcataataa
     9721 ttaccacgca ttggtttaaa gttagcaata aaacagccac tataattacg agtatataaa
     9781 cctccccaag gatacataga caagtctaat ccacctctag ctgcatattc ccattctgat
     9841 tctgttggta aacgatagtc atttaaatga tattctccaa ctgttctttg gtatgcattt
     9901 tttaacattg ttctccacat acaaaaagca tttgcttgag tccaactcac accaacaaca
     9961 ggataataat cataagcagg atgccaaaaa tacatttttg tcattggctc attatatgaa
    10021 taagtaaaat cacttaccca gcataaagta tcgggataaa tgtgtacttt ttcgcgtatc
    10081 caataactat atcttccacc acgacgacca gcatctgttt tgccatcgta atttttaatt
    10141 tctctgtctg caaaataatc ccaaggtcta tcttcatcaa gtttttcgcg gtcatcaatt
    10201 tttccttcat aatttccagt ttcaggatta aagtgacgac ctattcttaa accatgatca
    10261 ccttcacgac taaaagtacg agcaacatct tggtcaactc catcagcccc tggtctaaat
    10321 gtatgctttt gagctgcttg tttatagtca aaccagaagt agctataata caaagcatct
    10381 gtattcaagt cggtttgacg cataaaatga taaagttctg ctggaggcaa tagatataat
    10441 gtttgattga aattatttgg aatttcattt ttaattaaaa taccttttat ttcaagatca
    10501 ttataatcta gaggacctct ccagtctaga aaattataat tatcgtaatc atcaaaatca
    10561 aactcaccat tttcatacat gtttgtaatg aaattagact cttcttcggt aacatctgta
    10621 tcatcaataa taaaatctat cggctgataa gattttttag tactgatatc accaaaatcc
    10681 acactgtttt tggtttcttt acctaaaact gttaatgcca ttgaatctcg cacccaagct
    10741 acaaactgtc tgtattcaga atttgttatt tcagtttcgt ccatccaaaa accttgaaca
    10801 gtaactgttt tgctttgtgc agtcattgaa taagggacat cttggtcgct tggtcccatt
    10861 tgataacttc ctggaggaat gaacaacatt ccgtaaggat ctggcgtgta ccatactttt
    10921 ctgtctattc ctgtcagttc gccacttcca taataacgac agctacttaa agacactatg
    10981 attaccgcgc tgatgatgag taactttctc ataattattt tattttaagt aagtttcatt
    11041 tatacgtaaa aaattttaaa aggttacaat cgtctaaccc ctctatgtct tcctggtgtt
    11101 ttatccatag acaagttgaa gcaatatctt accattaact catgtgaacc caacatgttt
    11161 gaacctattt tgattaagct aatatcatag ctataagcaa aacttaatcc attaactaag
    11221 tgtacaccta tcattggtac tattgcatca ttatacctat aagataaacc tccccaaaaa
    11281 gatttattat ataatatttt tccatttatt tgaatttcag ggaaacctga gccatcagat
    11341 tgaaccattc ctgttccaat caaatcaaaa gaaggatttg gcaatgaata ttcataacct
    11401 ccagttaaat aaaaatgtct tgctatataa cttgggttat caatatcaaa cttaattact
    11461 ggattcaaca aatgtgttga tgataatccc acccaaaact tctctccaac aagagttgct
    11521 ccgaagttaa gatcgaaagc agtttttgac tccatatgtg gtatggttgg gtcaatataa
    11581 acaggttgcc caatcaaaga cccccaagtt ctccaattac cattaataga gcgattaatt
    11641 atacctgctc ctacaccaac tcctagcatt ccaaaatcca actctaaacg atatgcaaca
    11701 ttagcactta cattcaaatc tttttggaag ccaattatat cttgttgaat gtttaaacct
    11761 acacctatca tatcaagact tgggataggc atgtcaaaca agaacaaagt agtaactgga
    11821 cgtccttctt ctttaaaccc tacccattgc tgacgatgta aactagtaac acacatagca
    11881 ttattactac cggcagctcc tggattatat atattttgag aatattcaaa caagctaaag
    11941 tgtgggtctg actgagcctg cgaatattta acaaaaatac tcccgaaaaa tactattaac
    12001 aataaaactt tcctcatata attcttgttt atcactttca atacttaagt aaattttatt
    12061 aatttgtaaa taaaatacat tttttctata tctccaaatt ttattaacat ttttttgtta
    12121 atttaatgaa aaattgttaa taactttcat aaaacaaaaa ttcatttttt catttttact
    12181 tttttctaat catttttctg ttttaatttt cataaaacaa atcccaaaaa taataattat
    12241 ttcattaaac caacgttttt ttattatttt ttattattaa aaacataaaa acttattgtt
    12301 ttcgtcataa atttcgcatt tttaccttta ttttctacta aaaccaagta aaaattatac
    12361 ttttttactt tgagaaataa tagttttaca atcttccaaa ctcattgttt ctggatcata
    12421 agtttttggt attctataat ttttcttctc atatgaaata taaggtccat atcttccctt
    12481 taatatagtt aagcctggca tttcttcaaa tgtgcgacca aaggcttctt tttgttgaga
    12541 ttttttttca ttgataattt ctattgccct ctctttatta atggtataag ggtcgtcttg
    12601 cttttttaga ctataaaact ttccattatt ttttacatat ggtccatatt ttccaatagc
    12661 aactattaaa tcgtcttctt cgtatgtgcc aacatttcgc ggaagcttaa ataaatcaag
    12721 aacttcttct agtgtaacgc tttcaatatt ttgatttttt ctcaaactag catgtcgtgg
    12781 tggttctaca ccttcaatag aagcataagg tccatatttt cctattttca aaacaatagg
    12841 taaacctgtt tctggatgaa tacctatttc tctttcccat tttgtagttt ctttttcttt
    12901 taaactatct tcaacttgtt ttgaaaaatc ttcataaaaa tcagctaaca ttttattcca
    12961 agcaatttca ccaactgcaa tcttatcaaa ttcttcttca atttttgcag taaaatcata
    13021 actaactata ttatcaaaat gattttccaa ataatctgtt acaacaattc ccatggaagt
    13081 aggaaatagt ttattagctt cgccaccgaa tttttcctct tcttttttct tctctatctt
    13141 accgttttct aaaactaaat taacaagact tctagttttt ggactacggt tttcttttat
    13201 cacatattct ctttgttgaa tagttgaaat tgttggagca taagttgaag gtcgtccaat
    13261 tcctagctcc tccattttct ttaccaaact agcttctgtg taacgatatg gtggttgctc
    13321 aaacttttga tttgcttcaa tttttgaaac tatggcttta tctccttttt taaccttagg
    13381 taaaattgat tgagtttcgt ccatattttc atcgtcagta gattctatat ataattttaa
    13441 aaaaccatca aaaacaacaa cttcgccact tgcaacaaaa ctaatagtat cattagaatt
    13501 attaagtgta attatagttc tttctatctg tgcatcttgc atttgagagg ctatagttct
    13561 ttttcgaatt aaatcataaa gtttttgctc ttgttttgtt ccttcaatag tttcgtttgc
    13621 aacaaatgta ggtcttatag cttcgtgtgc ttcttgggct ccttttgttt tagttgtata
    13681 ttttcttgtt ttaacatatt cagcaccaaa tgtatcttct atcacttttt ttgaactgcc
    13741 tattgctaaa tttgacaaat taacagaatc tgttctcata taagtaataa aaccatgttc
    13801 gtacagactt tgagctaact tcattgtttg cccaacagaa tagcctaact tacgtcctgc
    13861 ttcttgctgt aaagtagaag ttgtaaaagg tggtgcagga gatttttttc caggcttttt
    13921 tgttatgtca gatacaacaa aatcttctgt tttacaagtt tctaaaaatg cttcagcttc
    13981 ttctaaagtt ttaaattttt tattcagttc agcctttatt gtatttttat caactttaaa
    14041 tatacctata attttataat aagacttaga tacaaaatta ttaatctcac gttctctttc
    14101 tacaattaat tttactgcaa cagattgaac cctgccagca gaaagagctg gttttatttt
    14161 tttccaaagt aaaggcgata aattaaaacc tactaaccta tctaaaactc gtctagcttg
    14221 ttgagcattt acaagatttt catcaatatc tctagggttt tttatagcat tttgaatagc
    14281 ttcttttgtg atttcatgaa aaacaattcg ctttacttca tcttttttaa ggttcaaaac
    14341 ttttttcaaa tgccaagata ttgcttctcc ttctcggtct tcatcggaag ctaaccaaac
    14401 tgtttttgca tcagctacat gtttttttaa ctctgcaact atttttttct tatcagcagg
    14461 aacttcatat tgtggtaaaa aaccattctc aatatcaatt cctaaattct ttttcgataa
    14521 atctctaata tgtccaaagc ttgatttaac aacaaaatca tcgcccaaaa acttcgtaat
    14581 ggtttttgcc tttgcagggg actctactat aactaaattt gtactcattt gaattgttaa
    14641 aatttttgtg ggtaaaaata aagaaaaact atatacatat taataaaaat aaatttaatt
    14701 ttacaccata aatttttaat atgatggaaa acaaaaatgc aaaggtaaca ttttctaaaa
    14761 aatttacgaa aaaattctta attacttttt ggacatgcat aggaattttt attgttgggc
    14821 tgtttattta cttcctttta ttatcaaaag gtgttttagg atatatgccc agctttgaag
    14881 aattagagaa cccaaaagaa aacttagctt cacgcgtcta ttcgagcgat ttagtcttgc
    14941 ttggaactta ttttcgtgaa aatcgttcaa acgtttcata tgatgaactt tcaccacatt
    15001 taatcgatgc actcattgct acagaagacg tgagatttca ttctcacagt ggaatagact
    15061 ttaaagcctt acccagagtc ttttctggaa ttttaacagg caataaaaaa ggcggaggaa
    15121 gtactatttc gcaacaatta gcaaaaatgc ttttccctag agaagaaaga ctttcaaaat
    15181 ttaaaatggt aaatcgaaaa tttcaagaat gggtaattgc cgtaaaactt gaaagaaatt
    15241 acaccaaaga agaaattata gaaatgtact ttaacaaatt tgactttcta aacttagctg
    15301 ttggtattga atctgcttca agagtttatt ttgacacaat tccaattgat ttatccatta
    15361 cacaagcggc tatgttagtt ggaatggcaa aaaaccctgc attatacaat ccgctaagac
    15421 gaccagaaga aactctacaa cgccgtaatg tagtattaaa tcaaatgcga aaatacggaa
    15481 aaattaccag agaagaatac gactctctaa aacaatgtcc attaggtatt agatatcaaa
    15541 aagttgatca caatataggt tctgcaactt attttagaga atttttaaga gcttggctaa
    15601 gcgctaccga acctcagcct aatgactaca ttgaccaaag agattatata gaagactcta
    15661 taaattggca tactgaccca tcgtatggct ggtgtaataa aaatgttaaa cctaatggtg
    15721 aaaactatga catatatagc gatggactcc aaatctacac caccatagac agcagaatgc
    15781 aaaaatatgc agaagaggct gtaaaagaac atattggcgg tactattcaa cccgaatttt
    15841 ttaaagacca aaaatataac aaaaattctc cttttacgaa tgacttaact aaagatcaga
    15901 tagaaaaaat aatgaatgct tctatgcgta gaagcgaaag atggagagtt ttaaaagcta
    15961 acggaatgag cgaagattct ataaaattat cgtttagcaa acccgaacaa atgagagttt
    16021 tctcgtggaa aggagaaata gatactgtaa tgacaccttt agattcaata aaatattata
    16081 aaacattcct acgtgccagt tttatgtcaa tggaaccaaa aactggatat gtaagagctt
    16141 atgttggagg catagatcac aaacatttta aatttgacca agtaactaaa tctagacgac
    16201 aagtaggttc tacgataaaa ccttttattt attgtttagc tatgcaaaat ggatattctc
    16261 cttgcacaca agttccaaat gtagaagtta catttaaact acccgaaggc tcggctgaag
    16321 agtattatac tccaaaattt tcaacttcaa agctagatgg tgaaatggta agtttaaaaa
    16381 caggcttagc attatcttta aaccaaattt ctgcttgggt tttaaaacaa ttttcacccg
    16441 aagcagtagt ttccattgct aaaaaattag gagtaaatag ccatatcgac ccttatcctt
    16501 caatttgcgt aggagcaccc gaagttttat taagtgaaat ggttgctgct tatgctgctt
    16561 ttgcaaacgg tggattatat acccgaccta tttatgttac aagaattgaa gaccaaaatg
    16621 gaaatgtttt agctactttt ggacaaattc aaacccaagc actgagcgaa gaaaccgcat
    16681 atctaatgat tgaattaatg aaaggtgtag tgaatgaagg aacaggttct agattacgat
    16741 ttagatataa cttaaactct gaaatagctg gaaaaacagg aactaccaac aacaattctg
    16801 atggctggtt tattggaata actccagaat tagtaaatgg tgcatgggtt ggtggagaag
    16861 aaagaagtat tagatttaga catatgactt tcggacaagg agcttcttta gcattaccaa
    16921 tttggggact atatatgcaa aaagtttttg acgacaaaaa actaccttat aatagcaaag
    16981 ctacatttac tcgtccaaac gaagtttcgg ttgaaaccga ttgtgcagaa tacaacagaa
    17041 atcatacaaa tataaatttt aacgagttgt aaaaactctt aactaacaaa aatcaagcta
    17101 ataaaaaaag gggctatcaa aacagcccct tttcttttta aaatttcgcg tatgaaaaaa
    17161 ttatttttta acgtattttg caacaaaatc tttaataatt gatttaaaat caggttgtcc
    17221 gatttcttgt aattcatact caacttttgc tgtaggtttg ttaattttca caactcttga
    17281 taaatcaatt ggagtagcaa ttactactgt atcacaaggt gtgttagcaa tagtcttttc
    17341 taagtctgct atttgctctg caccgtaacc catagctggt aataatttac caattcctgg
    17401 ataagtttca aaagtagtag ttaatttacc aactgtataa ggacgtggat caacatattc
    17461 tgcagcacca tattttttag cagctacagt acctgcaccg atagtcatat caccatgagt
    17521 tagagttggt ccgtcttcaa ctactaatac ttttttacct ttaatcaatt ctggtttgtc
    17581 aactgcaatt ggagaagccg cgtcaacaac aatagcatta ggattaactt tattaatatt
    17641 atctctaact tcttggattc cttctggaga agctgtatca attttgttaa taacaacaac
    17701 atctgctaat cttaaagttg tttcgccagg ataataagat aattcgtgac ctgctctatg
    17761 tgggtcaact actgtaacca tcatatctgt ttcgtagaaa gagaagtcat tgttaccacc
    17821 atcccaaagg ataacatcac aaccgtttgg atcattttcg gcagctctta aaatttcttc
    17881 ataatctaca cctgaataaa taacattacc tctaactaca tgaggttcgt attcttccat
    17941 ttcttcaata gtacagttat gttttttcaa atcttctaca gtagcaaatc tttgaactct
    18001 ttgagcattc aaatcgccat aaggcattgg gtgacgcaca gcaactactt ttattcccaa
    18061 ttccatgaaa gcttcaataa ttcttcttga agtttgactt ttaccgcaac cagttctaat
    18121 agcgcctaca gcaattacag gttttgtact ctttattgaa gtagctttag gacctaataa
    18181 tacaaagttt gcaccagcag tattaaccat tgctgcgata ttcataatgt gtctatattc
    18241 gatgtcgcta tatgcaaaca cgcattcatc aacattaagg tctttaatca atttttcaag
    18301 atctttttga tcataaattg gaattccatt tgggtataaa tcttcacctg ctaactcttt
    18361 tggatatttt cttcctgcaa tatcaggaat ttgagctgcg gtaaaagcaa ccacattgta
    18421 atttggattg tgtctataat aggtgttaaa attgtggaaa tctcttccag ctgcaccgat
    18481 aataattaca ttcttttttg ccataattaa tttagttttt aataaaaatt aaacattatt
    18541 ttcaattgcc acaaatatac aatttacaat tcaaaaaaaa gaatagtttt tactagattt
    18601 aatgtgttaa acaacatatt ttaaataaac atagctgaaa cgcttaaaat tattatctta
    18661 tatttatgga ataataaaat ttttgtatca aaatagtaaa caaaataata aaatttaaat
    18721 ttattgtttt gaaatgaaaa aaaaagttgt atttttacgt ttttaaattt tgacaattat
    18781 gcaaatcaaa aaatctaaaa aagcagacct agaatcaaga aaatcaacat ttttcttaat
    18841 cggtttaagt gtatcattag cactattact tctagttttt gaatggactt caaaagatgt
    18901 agtagcatcg tcattaggaa gtttaggagc tagtgatatt atagaagaag aaatagaaag
    18961 tactgtacaa gaacaagaac aaccaccaga agaagttgaa caaccagaac aagaacctga
    19021 aactattatt gaagaactta ctgttgtaga agatgacgta aaagttgatg atattaatat
    19081 taatacagaa gctgacgaaa aaacacaaac aagcacaaga gtagttcaag ttactgaagt
    19141 tgaaattgtt gaagaagaag ttgaaccaat tgcttttgcc gttgttgaaa caaaaccaga
    19201 atatccaggt ggcgaggtag ctttactaca atatatagcc gaaaacactg tatatcctcc
    19261 tgttgctaaa gaaaatggaa ttcaaggtag agtgtttgtg caatttgtaa tagacgctac
    19321 aggtagagtc acaaaagtgc aagttgctag aggcgttgac ccatatcttg acactgaagc
    19381 tgtaagagta gttaaaacac tacctaaatg gacaccaggt aaacaaagag gaaaaagtgt
    19441 acctgtaact tacatagtac ctattaattt taaattatat taaaatttac aaaaatattt
    19501 gtgcagaccc taccaaaatt atggtggggt ctttttatta ataatatatg ataaataaaa
    19561 atttttacat aagcgaagaa gatgctgttt tagttggagt tgcaactcaa tatcaaacaa
    19621 aagaagaagt tcaagaacat ctcgaagaat tagacttttt agcaaaaact gctggagtgg
    19681 ttcctaaaaa atattttatt caaaatttag atttacctca tcaaaaaact tatgtaggaa
    19741 gtggaaaact tctagaaata ttagattata ttactaaaaa cgaaattaaa ttagtaattt
    19801 ttgatgatga actatctcct acccaactta gaaatatcga aaatgtttta caagtaaaag
    19861 ttttagacag aacaaattta attctcgaca ttttcgccca aagagccaga acttcacatg
    19921 caaaagcaca agttgaatta gcacaatatc aatatatttt acctcgccta accagaatgt
    19981 ggactcacct tgaaagacaa cgtggaggta ttggtatgag aggacctgga gaaacacaaa
    20041 tcgaaacaga cagaagaatt atcctcgata aaatatctaa attaaaagaa gaacttaaat
    20101 ctattgacaa acaatttact caacaaagaa aaaatagagg acaaatgatt agagttgctc
    20161 tagtaggata tactaatgtt ggcaaatcaa caattatgaa tcttctaagt aaatctgatg
    20221 ttttagctga agataaactt tttgcaactt tagacacaac tgttagaaaa gttgtaatca
    20281 ataatttacc atttttatta agcgacactg taggttttat tcgtaaattg cctcacaaac
    20341 taatagaatc tttcaaatcg accctagacg aggcaaaaga atctgactta ataattcatg
    20401 ttattgatgt ttcgcataaa tcttttcaag aacaaattac tgttgttgat aatactttga
    20461 aagagctaaa aataaacgac aaacctatat taatggtttt taacaaaata gacgcttttg
    20521 aatattctcc acaagatgaa tttgatttaa ctcccaaaac taaagaaaat tattctctcg
    20581 aagattttaa aaatatgtac tttgcaaaaa caaacaatcc agcagtattt atttctgcaa
    20641 aaaacaaaga aaatattgaa gaatttagag aagtactttt taatcttgtt agagatttgc
    20701 attttgaaag gtatccgtac aatacaatat aaaaagctat aaaaacacag attttttcga
    20761 gaaaaactaa aatatttcaa aaatttttac taatattaca cattattata aatgttgttt
    20821 agatgaaact aaatatttta ggcggacgag gaagtctttt agaccaattt gttgcagaaa
    20881 ttcgcgacac tcaaatccaa caagacagaa tgagatttag aacaaatctt atgcgtgtgg
    20941 gagaaattat ggcttacgaa attagtaaaa acctagagtt tattgatgaa gaagttacaa
    21001 cttcgttagg aattgctaca gttccaactt taaaacaaca gccggtaata agcacaatat
    21061 tgcgagctgg actaccattt catcaagggt ttgtaaatta tttcgaccat gccgacaatg
    21121 cttttattgc tggctacaga aaatataata aaaatggaac cttcgaaata aaaattgact
    21181 acattaccgc cccttcaatt gaggacagaa ttgttataat aaatgaccct atgcttgcaa
    21241 gtggtggtag tattgaatta gcttataaag gcttattaaa tttcggaaaa ccaaaacatg
    21301 tacatgtagc tacagttatt gcaagtaaag atggtttgga ctatgtagat aaaatactac
    21361 caaataatat gattactgtt tggacaatag cagttgatga cgaattaaca gtaaaatcat
    21421 atatagtacc aggattaggc gatgcaggag atttggcttt cggtcctaaa ataaactatt
    21481 aaatattatt tttaccatac ctatttatat taatgcaaaa tcttaaatga ttttgttaat
    21541 tttgcaaagt ttttaaaaat gacaaagaat atgtatagaa cacacaattg tggcgaattg
    21601 cgtagcaaac acattggaca aaaagtaaaa ctaagtggct gggttcaatt aaaaagagat
    21661 aaaggcggaa tgatttggat agatttacgc gacagatatg gaattacaca actcgctata
    21721 gaagaggcaa atacctctaa agaaattatc gaaactatag ataaaatagg acgcgaatat
    21781 gtaataagtg ttactggcga agttattgaa agatacagca aaaacgacaa aatgccaaca
    21841 ggagacattg aaataaaggt tgaagaaatc gaaattctta acgaatctga aacgccacct
    21901 tttacaatta ttgacaatag cgatggcgga gacgaactta gaatgaaata tcgttatctc
    21961 gacattagac gaaatccagt aaaaaacgct cttttacttc gccacaaaat ggctcaagaa
    22021 ataagaaaat atcttgacga aaaagatttt gttgaagttg aaactccttt tctaataaaa
    22081 tcaactccag agggagctcg cgattttgtt gttccatcac gtatgcacca aggcgagttt
    22141 tacgctcttc cacaatcgcc tcaaactttt aagcaattgc taatgattgc tggaatggac
    22201 aaatatttcc aaattgtaaa atgctttaga gacgaagact tgcgtgccga ccgccaacca
    22261 gaatttactc aagttgactg cgaaatgtct tttgtgaacc aaaatgatat tatcgcaact
    22321 tttgaagcaa tgacaaaaca tttattcaaa acaattaaaa atattgatat agattacgat
    22381 tttccacaaa tgacctataa ggaagcaatg gaaaaatacg gaaacgacaa gccagacatt
    22441 agatttggaa tgacaattac cgatattaca aacctagtaa aaggaaaaga ttttgttgtt
    22501 ttcgactctg ccgaatatat aggagctata gcacttgaaa acgcaagcga atattctcgc
    22561 aaacaaattg atgcacttac tgattttgtt aaaactcctc aaattggggc aaaaggatta
    22621 gtatattgta aatacagcac agatggaagt tttaaatctt ctgttgacaa attttttaac
    22681 gaaaacgatt ggacaaagat aagccaagcc atgcaagcaa aacaaggcga tttaatcttg
    22741 ataattgcag gcgaaaaaga tgcaagtctg cacgctttaa gcgaattaag attaaaatta
    22801 gctgatgaaa atggtttgcg ttcaaaagat aattttgctc cgctttgggt tatagaattt
    22861 ccgcttttag aatatgataa agaaaccgat agattttatg caaaacacca tccattcaca
    22921 gctcctttcg acgaagacat aaaacttttt gacacagacc caagtaaaat tcgagctaaa
    22981 gcatacgacc tcgttataaa tggagttgaa atcggtggtg gctcaattag aatttttaat
    23041 aaagatttac aagctttaat gtttaaaaat cttggttttt ccgaagaaga agcacaagaa
    23101 caatttggat ttttaatgaa cgcttttaaa tatggagcac cacctcacgg gggaattgct
    23161 ttaggatttg ataggttagt ttctatgttt ggaggaagcg attcaattag agattatata
    23221 gctttcccaa aaaataatgc aggaagagat gtgatgatag aaagcccatc tacaatctct
    23281 caagctcaac ttgatgaatt aggtctaatt attaaaaaat aaaacctaaa atattttgta
    23341 ccttatttac tcaaaattct atttgagata ttttaatttt ctatgctttt gtctaagtta
    23401 ttttattatt tttgtatttc tatcaaaaca atattgatat ggctaagcaa aaaactaaac
    23461 ataatttaaa acagaaacct ttaccacagc aagaaattaa aaaagttaca aaaaataaaa
    23521 agtataattt atttgttttt ttaacaatta gcgtttttgc ttttattctt tatgggaata
    23581 ccctctcaca cgattatgct cttgatgacg caattgttat aactgaaaac acatttacgc
    23641 aaaaaggttt agctggaatt tcagatattt taaaatatga tacctttgtt ggtttttggc
    23701 ttacaaaaaa tcctaacaaa actgccgaac aaattcaaga agaaaaaaaa cttgtcgctg
    23761 gaggacgtta tcgcccccta tctttaatta gctttgctac agaaatagaa ttttttggta
    23821 aaaatattaa aaatgatgca ggaaaagttg tgtataaaga aaatcccttt gtcagccatt
    23881 tggggaatat aattttgtat ttattaacta cttacttgct atatctaatt ttggcaaaac
    23941 ttttcccacc cgatgaaaat aaaaaatggt atttaagttt tccttttatt gcttctttgc
    24001 tttttcttgc acaccctctt catactgagg cagttgcaaa cataaaaggt agagacgaaa
    24061 taatgacatt gcttggttcg cttggagctt tatggtttac tctaaaatat ttagatacta
    24121 aaaaagcaaa atttttagtt tattcttctc tttcattgtt tttaggacta ttatcaaaag
    24181 aaaacgcaat aactttttta gcaataatcc caattacagt ttattatttt acaaatcaca
    24241 atttaaaatc aaactttaaa tcattattgc ctttattgct tgtttcgggt gtgtttttac
    24301 ttattcgtgg ctccgtactt ggaatgggta caggcgaaaa acaaattgct caagaaatta
    24361 tgaacaaccc gttttttgga gcaagtgtag gagaaaaatt tgctacaatt ttctttacac
    24421 tttggatgta tattaaactt ttgtttttcc ctcacccact aacttacgac tattatcctt
    24481 ggcaaataga aattataaat tggtctaatc caaaagcatt tttaccatta atcttatatt
    24541 tggcactagg aatttatgct ctgtatggat tgattaaaaa gaaagatatt ttttcatact
    24601 caatttggtt ttatttaatt ccactatctg ttgtttcaaa tatatttttc ccagttggaa
    24661 catttatgaa cgaaagattt atttttattt catcaatagg attttgcatt tttatagcaa
    24721 ttttattaac aaaatattta aacaaactca ttaaaaacac acaaactcat tcttactttt
    24781 tggtattaat tgcagttagt attttaggtt tatattcgtt taaaaccatt gatagaaaca
    24841 aagcttggaa aaatgattta acacttttca caactgatgt taagacttct acaaactctg
    24901 caaaaagtaa ttgttctgcc ggaggcaaac ttattgaaga agctcaaaaa ccaatgtata
    24961 aaaacgatac agcaactcgc aaccaaatga taaatcaagc catagtttat ttagaaagag
    25021 cattagaaat ttatcccgaa tatattgatg ctataaatct tttgggcaat gctcattatg
    25081 aattgaatta taatattaac aaggctttat ataattacgc taaaatttta aaactaaaac
    25141 catatcatag cattgcatat ggaaatgcaa gaattgtttt actaaacacc attggtttat
    25201 taaattctca agcaacagtt tccactccag atgatgtaat agcaggctgt aacgaagttt
    25261 taaaagcaca cccagatttt ggtgaagcaa atcacataat gggaactatg tatggaaaat
    25321 ataaaaacat gcttgattct tcattatttt atttagaaaa agctaatcaa tgtaatttta
    25381 ataaagatgc aggtttttat cgcgatttgg gtgttgcata cggcatgtcg ggaatattta
    25441 aacctgctct tgacaatttc ctcaaagcta tggaattaga cccaaacgat tatcaaacct
    25501 atatgaatat tggagttagc tatcagcaac ttggagatat tgccaatgca aataagtatt
    25561 atttaaaagc tcaagaattt caaaatataa acactaatca atgataaaag ataaaaaaat
    25621 agttgtcgta cttcccgctt ataatgccga actcacggta gaaagcactt ataatgaaat
    25681 acctttcgac attgttgatg aagtaatttt agttgacgac aaatcaaaag acaaaactgt
    25741 tgaaaaagct aaagaacttg ggataaaaca catttttatt cacgacgaaa ataaaggcta
    25801 cggagcaaat caaaaaacat gctataataa tgctttaaaa ttaggagcag atattgtaat
    25861 tatgcttcac cccgattatc aatatacgcc aaaattaatt cactcaatgg cttatcttat
    25921 tgcaaatgat gtttatcctg ttgttttggg ctctagaatt ttagggaaag gagctttaaa
    25981 aggtggaatg ccaaaataca aatacttttt caaccgtatt ttgactttta gccaaaatat
    26041 tttaatgaat caaaaacttt ctgaatatca cactggtttt agagcttttt ctgccgatgt
    26101 gctaaaaaaa ataaattata atattaattc cgatgatttt gtttttgata atcaaatgct
    26161 tgcacaaata ttttatgctg gctacgaaat tgcagaaatc acttgtccta caaaatattt
    26221 tgaagaagcc tcatcaataa attttaaacg tagtgtaaaa tatggtatgg gcgttttaac
    26281 aacttcatta aaatattttt tcaacaaatt aaaacttaca aaatccgata tttttaaacc
    26341 aattgcttct gaagaataat gcaaagttta gataagatac tcgatattaa atatcctgtg
    26401 attttagcac caatgttttt ggttactacg ccagaaatgg tgagaattgc tttagattgt
    26461 ggtattactg gagcaattcc agcaatgaat tttcgcaaag aaagcgaact taaagctgct
    26521 atagaagatt tgaaaaaaca ttccgacaaa gcttttggaa ttaatcttat aacaaatcgt
    26581 tcaaaccaaa aatataaaaa acaacttaaa gcaattttag aagcaaaacc agcttttgta
    26641 atttcttcat taggaaatcc taaagaactt attacagaag ctcataaagt tggaataaaa
    26701 gtattttgcg atgtggttga cctaaagttt gctaaaaaag ttgaagactt aggtgccgat
    26761 gcaataattg cagtaaactc ttcggctggt gggcatagcg gaattataga aagagatgaa
    26821 ctaataggtc aacttgttgc taattgccaa attccaatta taaatgctgg tggtgtaagt
    26881 acaaattcag acttaaatca tgttatgtct cttggagctg caggagcttc ggtaggcaca
    26941 atatttatgg catctcatga gtgtcatatt tctgacgact acaaacaagc attaattaaa
    27001 tatggaaaag atgatattgt acttactcgc aaactttcag gagttccttc ttgcgtaata
    27061 aaaacagaat atgtcaaaaa acttggaact aagcctaata ttttagaatt gctaattaaa
    27121 aacaacactt ttttaaaaaa atatgcaaaa tatttaatta ctaattttgg aatgaaaaag
    27181 ttagaaaaag cagctttttc ggcaacttac aaaaacttct gggtagcaag taaagccatt
    27241 gaaaacatta aagaaataca aaatcttagt gtgataatta aaaaattagt acagccaaaa
    27301 taaaaccata taaatatttt tttgtaattt tgaacgtaaa taaaaattat tactaaaatg
    27361 aaaaaactta aagcaatatt tatcttatta tttgtagcat ctataagtat atatgctcaa
    27421 aattatgatg atccacaaaa tattgatttg caatataaca gagatgcaga atatccaggt
    27481 ggcatgaata aatttattac cgacttatgg aatagcatgt catatactca agaagctatt
    27541 gatgctcgcg ttgatggcac aataacaata agttttgatg ttgaagcaaa tggcgaagtt
    27601 agtaaagttg acgttattgg tggacttgga atgggcattg acgaagaagt tgccaatgtt
    27661 ataaaagcca tgacatttac tcctgctctc gttgaaggca aagctgttaa gatgaacctt
    27721 atgtttactg ttccaattag agttggtcct aattcgagac ttaaaaaata aaaaaagcgc
    27781 aggttttgtt acctaagcct cttctgtttt cagcgttgca acacccaaac acaataaatc
    27841 gcctgattaa cagagactta gcgggtgttg caaaaagtgc agaaaacagc tcagcaaagc
    27901 taaaatccaa acaaaaggag ggaaaaacac aaaaatatag caataaaaat gttttaacat
    27961 atttttttgt tttattaaat attttttata aatttggaaa cgaagattga tttgaagcat
    28021 ttggataaat tataaaaaaa ttaacactat gaaagcacca aaaacattat ttacaatttt
    28081 aattttattg cattgtgcaa tagtattttc ttaagttgga aactcaaaaa gggcttggca
    28141 ttggtatttt ggttggaatg cagggcttga ttttagcaat ggaactgaac tatgcaagaa
    28201 gaccttaatg ggcaagttta tatagacgaa ggagcttgct caatatcaga tacagcaggc
    28261 aatttattgt tttatgcagg accaaatggc ggaaccttac ccggtgaaaa caatatgaca
    28321 gtttataatg ctaaccatga agtaatgctg aacggaacag atatagactg cggatggagt
    28381 ggacgacaaa atagtattat tgttccacta cccggaaacg ataatattta ttatttattt
    28441 acaattggag atgtggctgt agttgaatac aatatggacc aacctggatt taagtacaat
    28501 attattgata tgagtttaga tggtggttta ggagctgtta cagaaaaaaa tattgaaatc
    28561 tactccaatc aagcaaatga tacactaagt gaaatattaa cagcagttca tcatgctaat
    28621 tgcgaagatg tttggatagt aatacacaac tatatgacag atgtgtttat ggtttataaa
    28681 ttaacagctg acggcttaga ttcaaatgta gtaattaatc aaataggaaa ttctacatgg
    28741 ggaaattacc acacatgggg ttggggaatg aaattttctc ctaatggtaa atatgccgct
    28801 gtaaataaaa atttcatgtg tccatcaaat tgtcaaatca aagatacttt agaattatat
    28861 agatttgata acgaaactgg tattttatca gatagaattg caatcccaat ggattcggct
    28921 gtagcaggta aagagttttc atttacaaat gatttattat atacttttga agcacatata
    28981 tatgaattag aaattcctcc gtcaatttat caatatgata ttagtaaatg gaataaatca
    29041 tatattatgg attctaaaac tcttattgga aattattttt tcccaacacc agatttacat
    29101 cttacacctt tgaatacaat tattataagt ggagaacttg catttaaccc gcctgaagga
    29161 tatataggag taatagagaa ccctaatgtt cgaggtattg gttgttcgat aaatgaaaat
    29221 gcgttttata tttcacgaaa ccctggatgg aatttcccaa attttgttcg ctcttatttt
    29281 aataccgata tttctgttta taattgtaat acagttactg ttgaagatgt aataaaaaac
    29341 caagcttttg aaatatatcc caatccattt tctaaacaag cagaaattaa aacttacgat
    29401 aaagaaatta tatattatga tttatttgac ttaaaaggta ataaattatt aaaagataaa
    29461 gactataaat taaggcaaat tgataatgcc tatatatttg ttaatgaaac acttaaagaa
    29521 ggcatatata ttaacttgca ggttctccga caacaaattt tttaatctga aacttagtgt
    29581 tattaattga aattatgaaa aaaaatcacc tgttttcagc cttgcaacac ccatgcacgc
    29641 caaatcgtct gattgtcaga gatttagcta gtgttacaaa aagtgcggaa aatagaaaaa
    29701 gataaaaaaa agcgcaggtt ttgttaccta cgctttctat ttttttatct acaaaaatta
    29761 cttcttaata atttttttca agatagtatc tttgtctaat tttttagtac agaagaacca
    29821 gtctttgcaa tctaaatctt tttcatcgaa tcttgcttgt gctgcactgc aagtagatgc
    29881 aggagatact attacatcat cacctggttg ccaatctgct ggtgttgcta cgccaaactc
    29941 gtctgatact tgtaatgcta tcaaagcacg gtaaatctcg tcgaaattac gacctaaact
    30001 ttgaggataa taaatcatag cacgcaaaat accttttgga tcgataaaaa atacagctct
    30061 aactgcttga gttgcacttt cgtcaggttg aatcattcca tatttccttg aaacattcat
    30121 agaaatatca tcaattaaag gaaatttaac ttctacattt ttcattcctt tatactctat
    30181 tttttcttta attgttctca accaagcgat atggctatgc aatccatcaa ttgaaagtcc
    30241 taccaattca caattagctt tattgaattt ttcttctaaa cttgcaaaag tcataaactc
    30301 tgatgtacac actggagtaa aatcagcagg atgactaaat aaaatcaccc attttccttt
    30361 ataatcagct gggaaattaa tttttccttg agttgtgttt gcggtaaatt ctggagccgg
    30421 agcatttaat cctggcattg tgtttacttg ttcgttaatt ctttcttcca ttttaattta
    30481 atttttacgg ttaatattta attttaattt attcactaat atatttccct ttgatttgta
    30541 acttaacgtc tttaatttca aatccgggaa ttgttttatt tcgaaaatat tcttctaaaa
    30601 tagagtttaa ctcatcgtca aaataatctt caatttttcc tgaatcaatc tcatacaaat
    30661 gatgaggctt ctcttgtcta gtgtctagca tcaaatctaa acttaccatc ttctgacaga
    30721 acttttgaca ctagtcgttt ttgcaccaat gtctcaatat tattatatat agtcgctaaa
    30781 gaaagccctg gcaattcttt tttcaaagct tctgcaattt cgtctgcaca aggatgattt
    30841 aactcattta aaacctttaa aattgcaact cgttggggag ttacttttaa tccagtttct
    30901 tttagtttcg atatataatt gtttgtattc atttctaaat aataataatt cttatttaag
    30961 aactattaaa tcaacaagct aaagcataaa atgttcaaca agtaattgca ttttatacaa
    31021 aacactattt ttacaacact tttaaaatat tagattatac ttttagcgta tgacaaattt
    31081 tatattatca aaatcctgcg agttcaacaa caattatatc agattttgta acagccttaa
    31141 catcaatgct tttattgtta gacaaagttt ctaaaaaaat aacatctcca ctttctacag
    31201 ttttatcgca tatttgcaat ttgccagaat aaatataaaa taaagccttt tgagttttta
    31261 cattaaaatt aactgtttta gtattttctg tttcaaatcc caagtgtagc atatgaccat
    31321 ttacatttcc ttttgtcatc aaattaaaat ctgtagcttt gccaatgctc cgagtattcc
    31381 actctccaga aaatgtatca gtatcaaatt tgtctaagtt tttggagtac ctaccctcgt
    31441 gaattagctc gatttttccg tctaaaatca ttattttgcg attaacttcg tcaagttttg
    31501 taaactgaga ctccttaatt tcaatagttg cggtacttac tcgaaacagg aaatttcgct
    31561 tttgatactc actttccctt gggtagataa aaagttctgt tgtagttccg cctgaccagt
    31621 ttgttgtttt atgagattgt ttttgtatta caagcatctc catattttat aaaaattaac
    31681 caaaaattaa tctttttatt tcgccaatcc acaaaactaa agaagttgca ccaaatatta
    31741 taagccaatc aacaaacgaa attggtactg ttctaaacat ttcaccgcca aattgtacta
    31801 tgaatatttg tcctactaaa atagctagtg ctacagttaa aaatgcctta cttttgtgta
    31861 gcgatttcag agcagatttt cctgtaaata aagcttttac attaaacata ttccaaaact
    31921 gtagcattac aaatgtagtg aaaaacaagc tcaaattata tatagagata tttccatctt
    31981 cgtctgtaaa ataatataat agagctaaca atataacgac aaaactaagt cctgttataa
    32041 aaatactgtt cctcattgtt ttgtttataa taaattcatt gctttttctt ggtttattat
    32101 tcattacaga tttatttggt ggtaaactag caaatgctcc tgctgcgaaa gtgtccatta
    32161 ttaaattaat ccacaacatt tgagttatag taagaggaat ttcatgtcca acaatagaac
    32221 cgataaaaac aataagcatt gctacaagat ttatacttaa ttggaatact acaaatcttt
    32281 gaatattttg atacaaagtt cttccccaca aaactgctga gcctatactg tcaaaagagt
    32341 cgtccataat agtaatgtcg cttgcttctt ttgctacagc agttccacta cccatagaaa
    32401 gtccaacgtg agcaaaattc aaggctggag catcgtttgt tccgtcacca gttactgcaa
    32461 ccacttctcc caactcttga agtaactgaa ccaatcgttt tttatctaaa ggtctagcac
    32521 gacataatat tttaaggtct tttagtctca acaataattc ttcatcagtt aaactactaa
    32581 attcatcacc tgttagaact gctttttcat cataatcagc atttaacaat cctatttttt
    32641 tagcaatttc aacagcaatt ccataagtgt cgcctgtaac aattattata tcaattcctg
    32701 ctttaagaca agttgctact gcttcgttaa catcatctct aattggatct tggattccta
    32761 caaaaccata aaaagttaaa ttatgattta ctaattttgc atcttcaatt ctaggagtat
    32821 catcttctaa tatttcataa gcaaagccta aagttcttag tgcttgagaa tggttttttg
    32881 ccaaaattgt ttttataaga tcactacatt ctgaatttgg tttaactccg tcttcgcaat
    32941 aaacttgctt cgacatatga aaaagtattt ccgaagcacc ttttacatac aaaactttct
    33001 tatttaagat tggcgaaaac acaacactcg ccatgtattt tctatctgtt gaaaaactca
    33061 attgttctag aacttctgta gcatttctaa ctttttcata tgggcagtgc attttatgaa
    33121 gccacaacaa caaagcagat tctgttggat ttccaacacc ttttattttt gtttcatcag
    33181 aataatctaa atctgcagtt gagtttgcag aaatattttc ttttataaaa atcaaattat
    33241 catcgccacc attaaaatcc aaaacttctc ttccaaaaaa catagcttca acaacttcca
    33301 tttgattttt tgttaaagtt ccagttttat cggtacaaat aactgtacaa gctcccatgg
    33361 tttcggttgc ttgcattttt ctaaccaaat tatttgactt cagcatattt ctcatactca
    33421 aagctaagca aagagtaacg ctcattggta aaccttcggg aacagtaaca acgactaaag
    33481 ttacagcaac cataaaatat tgtaaaatcc ttgaggcagt aggaatatcc atccaagccg
    33541 atttttctaa aacatgaaaa tcgaaaatca aggacactaa aacacttagt atcaacacta
    33601 agagagcaat aacaaaaagt ttgattgttt ttattttttc aactttttct aaaaacacac
    33661 tttttttctt aaaaagcatt tctttaaaac tgctaaaata tggaaaccac aattttaaac
    33721 taagaatagc aaaaaatata ataatgctta taaagaaata tgattgttca aaagttaagc
    33781 taatcttttt taacattaaa tctttaatca gaagtgcaaa aaaagttaaa atagctacgc
    33841 tagtaccgac aacaccaata aatccagcta acttttctaa ttgaatatta agtggagttt
    33901 ttacattaga tatttccaca gagctttttg ctacttttcc atattctgta ctatcaccaa
    33961 ctgcgtcaac tacaaaaatt ccgcttccat caagaatttt agaacttcgc aacaccttat
    34021 tatatgggaa agtgctttct tttttaaatt cattgagatc tacgcctttt ctcgtagatg
    34081 gttcacctgt caaactcgat tcatctacta ataaagaaac agactctatc aaaatcccat
    34141 cagctggcac ttcatcaccg acagacaaaa taacataatc accaacaaca atatctcctt
    34201 ttctaacttc ggtaacaaat ccgtctcgaa taacttttac caaagtatca ttatttacta
    34261 aatttaaaat atcgaatttt ttgttagcat catattcaaa ccaaaagcct atacctgttg
    34321 ctaaaaaaat tgcaaataat attccgatag cttcgataaa ttgatcgtta acaaaaccaa
    34381 ttccaaaaga tattaaagca gcaattagta atatttttat caaagggtca ttaaattttt
    34441 ctaaaaacaa tttaaaagca gacctttttt tatgctgtgt tataagattt gacccataaa
    34501 cttgtcggtt ttgctcaacc tctgttgttg ttaaaccctt gtattgcata gttttttata
    34561 tttatatatt tttttgactg aacgttcggt caaaaatagt aattatttta atacaacaac
    34621 acaaattttg cattttttta cattaaatat gaaatcttaa tggttagata taatttctag
    34681 gatttaaaag tcaataaaga attaattttt tatctttgta tttttaaaac taaagactat
    34741 gaaaatattt tttaaaatta aagtaacatt tatttttgca cttgttgcaa attttgcatt
    34801 ttcacaagat tggactgaaa gattaaaaat aactgctaac gaaagagcaa acaatgctct
    34861 ctttggtgct tctgttgcca taagtggaga ttttgccatt ataggggcta atcaagaaaa
    34921 aaaagatgca gataatagtg aagaaaatat tttaacagat gctggtgctg cttatatttt
    34981 caagaatgaa agtggagaat ggatttttta ccaaaaaata gtagcaagcg atagaagtgg
    35041 tggagactat tttggtcatt ctgttgctat tgatggtgat tttattgttg ttggcgctcc
    35101 acaagaagat gaagatgaaa atggacaaaa cacaaaatta aacactggtt cggcttacgt
    35161 ttttaaaaac attggtggca attggacaga aatacaaaaa atttgtgcat ctgaccgaaa
    35221 cacacaagat tggtttggtt gttctgtagg aattagcgga aatgacattg taattggtgc
    35281 ttatgcacaa ggcactgatg aaaatggtgg aaatcaaaca gatatggctg gagcagttta
    35341 ctattttaaa aatcaaggag gaacttggac agaaacacaa aaaatggttg ctcatgatag
    35401 aagaactgct gattattttg gtgcttcgct tgctatagaa ggaaacagat tagttgtggg
    35461 tgcttatttg caagattaca acgcaaatgg ttatacaccc aaaacaaatg ctggtgcagt
    35521 ttattattta gaaaaagaaa ataacacttg gactataaaa caaaaactag ttgctgatga
    35581 tagaaaagct gaaagcaatt tcggatactc tgtttctata agcggtgatt atatcatagt
    35641 tggcgctcat tatcacgacg aaagttctag tttaacatca agtggggctg catacatttt
    35701 taaatatagt tcaaacactt gggaacaaca aacaaaacta ataccttcag atttagaagc
    35761 caatgataga tttggtcaat ccgtagcaat aagtggaaat tatgcaattg ttggtgcaga
    35821 tcttgaagac cacgatataa atggcggaaa caacttttct aatgctggtt ctgcttatct
    35881 ttttgaattg caagataata actgggtaca aatacaaaaa ttatgtgcta gtgatagaag
    35941 tttaaatgat tcttttggat ataaagttgc aattcaaggc tataatttta ttgttacagc
    36001 aaaccaagaa gaccatgata taaataatca aaactattta aacaattctg gctctgcata
    36061 tatttttaga aagaaatcaa atcttattgt taaaaaagat aatgagatta taaataatgg
    36121 acaaacttta gattttggaa atatagtttt taacgatgat acacttacaa ttagtttaaa
    36181 tatttcaaca gacggcggtg actatatttg ttttactaat gaaaatcttt ttgtaattca
    36241 aggtaataat gctgataatt ttgcacttga atttgacaaa aatattgact catgcttaaa
    36301 cccagtaagc acaatcaact taggtgtgaa atttatacca ataagtcaag gattaaaaac
    36361 tgctcagcta ttattaacta atgattctga acaaagtaat tttaaaataa ttttaaaagg
    36421 taatggtatt gcatcttctt cgataacaaa tattagccaa aacatttcaa ttttcccaaa
    36481 tcctgctagt tcgcaaattt ttattgattt aggaaaagta aaagttgatg ttttaaatat
    36541 ttatgatatt agcggaaaat gtcttataac acgtaccaat ttaaaagaca taatcaacat
    36601 cgatataagc aacttacaaa gtggtgttta tattttaaat gctatttctg ataatgaagt
    36661 tttaaacttt aaatttattg ttgagtaaat catttaggta taacattttg atagtgttta
    36721 taccttttga caattgattc tgcaaagctc acagcatata tacctggata gtatccatga
    36781 attaccacag gatctttata gtattttgca tttgacatat tagaaagata aaaagcaact
    36841 gactcccaag aagagacatc atctttgtac ttttctgcaa gtctcatagc gtcttcaaca
    36901 tgtcctggac ctatattata agatgccagc acaaaataaa ttctatcttc ttgtttagga
    36961 acttgctcta aaaacttttt atcaagaaac tctatatatt ttactcccgc ataaatttga
    37021 gcatcaatcc ctgatttttc cggatcttta aaagtattaa atgttactgg cattagctgc
    37081 attagtccat aagcacctgc ccaacttact aaatcagggt taaatctaga ctcttcataa
    37141 attaatgacg atactaaacg ccaatcccaa tttattaact tactgtattt gcgaatttta
    37201 tcatcatatt cactaatttt tcctgtgcta atggaataaa aatcactttc taagtcaacc
    37261 aaatttttat tattgtttat atattttaga tttaaccttc tgtaatcgtt tgattgcaag
    37321 aaatcttcaa gccaaaaatt cacactatct aataattgtt tagattctgg acgaagcgac
    37381 caagaaatat ttttacttac acttacatct gtggaataat cgatattgtg ccaataactt
    37441 tcactaattt tcgcaatatt ataatcgaca actgcaaaat caaaaattcc ttctgaaaca
    37501 ttttttatta ttgcttcgtt agataaagaa tcaatttcta ctaaataaaa attaggaatt
    37561 atttctgtta aataagtttt taaaactttt atatctgtct gatttttagc gacataaata
    37621 tatttaagtg ttgtatttcc atctgtatct ggctttaaat ttaatatatc ttcatttctt
    37681 ggaacgtgtt taaccaaaac taaacgtgta gaacgaattg gcaaagtata attaacataa
    37741 gattcttgac ttaaagagtt tataattccc tgagccaata tatcgcattc tctgtccaac
    37801 aacatttcca aacctttttg ggcatcgttt tcaattttta ttactaactc taagccttta
    37861 gattttgcaa aactttctag catttctaac tgaaaaccca ttggctgacc ttgataaata
    37921 aaatagtcga taggatttgt tgctaaaaca acttttaatt ctttcgattc tattatttct
    37981 tcataatctc gtttactttc ttcaatacca aaaccaattt taaagtaatg caagacagca
    38041 aacaaaccca aaattagaag aattacaaat aatacaatag taaaaattct aaaacttttt
    38101 atatgacttc ctggtttagg gctcatgtaa aaattatttt tttacaagtt aacaaaaatt
    38161 atccttatat agattaattt aattgaaaaa ttttcaacaa tttatgaatt aatcataaaa
    38221 ctcccacgaa attccgaaat taaatgagaa tggattatct ggcattttat gcgaaacaaa
    38281 ataatttttt tccataataa aagaattgaa attactaatt cttacaaaac ctctaagtcg
    38341 tttaatgagt acactaatat tgaaagccat attaggataa tttccatact tacgctctgt
    38401 ttgcaaataa aaagtactta aagcaggatt ataagcatat aaataagttt ttgaaaaata
    38461 tttaacatca aatcctaatt gtaaaatcat aactttttta aacattggcg actgaaaata
    38521 aatcgaagaa taagcaacaa aatctggtag cggtattcga cttttatctt taatgtattg
    38581 ataactcaac tttgaaagcc aatgaaactt accaaaatca aaacgttttg ctagccaagc
    38641 atctgcaata aaatttaatt gagcaacttg aactggcata gattcttggt taaaaacaat
    38701 atagttttgt aataaattag tgtttattcc tgtagaaagt tttatgtttt caaaattcaa
    38761 atttgtattt gcagcaaaag tttgtgtctt ataaaaatca ttttcccaag cataatgatt
    38821 tgaagaatag tgatatataa atttatcgat attttttgaa ttaatattta ctcccgattg
    38881 ccaagttgtt gattttccaa tatctaagac atacttgcca gatactccaa aatctccaat
    38941 atcagtacct ataaaacaat aatctgcttc aaaattcaaa tatgatttct cagtttctaa
    39001 aattaaattt ccaattaaag aattagaaaa atacaaatat gatttaagag tatctatgtc
    39061 ataattatat aaatatgatt ttacacctgc taaaacttta aatttttcta tttttcctct
    39121 ttgagcaata ttaaaattta acagtatgtt attatccaaa actttatatg ccgatgaatc
    39181 aaaagtgttt tctaagttga aatatgtatt tggataataa gcagatggtt tatcttgata
    39241 aattctaaaa tatctatcag cttttaaatc atgaataatt gaaaatttgc tatcaaaagt
    39301 tttattcaag ctagtatctt ttccttgaac aattgtatca acatcatatt tcccaaagcc
    39361 aaactcctga ttgtattgaa atccaagttg tgaaactgta ttttgtgcct gcataagatt
    39421 tgttgcataa gttcccgccg aataactaga gtttaaatat ttatcaatat cactcaatcc
    39481 tccattttct aagttattta ttttgttaaa aataaaatta aaatgagact gatactttcg
    39541 tttagtataa gcagttgcga gacttattgc attaatttta gacttagaat attgataata
    39601 tccgtcagaa ttaataatat cataattaaa tacgaaatta aatgttggcg aaatgttttg
    39661 tgtatgtaaa aacctaacat actcctgccc ttttccacca ccggcaaact taaagagtgt
    39721 aaaaggtttt ttggcatcaa agtaaacaat ttcgtcgtgt tgtctaatgt agggtatata
    39781 attatttaaa aaccaatagt cgtgagtttg cttttctgaa aataaagatg gataaaaagc
    39841 tgttccaaga tttggtagtc ctaaaggatg caagctatat ttttgctgag gtaagtagag
    39901 aaaaaaagaa tctgtaagtg tgtctttatc gtaagcatca tcagcacaag gtaagttgta
    39961 gagcttatat gatgtaaaat gcctttcaat attttcgtgc tgagagaata aagaataagc
    40021 aaactgtaca aacaatatta aaacaagtat ttttttcacg cttcaaagat agaataaaag
    40081 tttttagttt ttagttttta attatgagtt tttagttttt agttaaactt ttatctatgg
    40141 agagcgtaat tcaaaatcaa ctaattagtt ttaaaatatt ttttaattaa aaacaatatc
    40201 ctaatcccag cccaatattt atacaagagc cattgctaga aattgtgtaa aaattttgtc
    40261 caccaccaac ttcgttaatt aaaatgctat tattctccca aactttatta attccagaat
    40321 aataatttgc aaaaatatct atttcaaatt tagatttaat taaataggat attccaattc
    40381 cattaacaaa accaaaagac aaattgtttg tgaaattata atctgaaaca ctacctgtaa
    40441 tattgcttgt tgaacttcca atgattctgt tataagtgta tgaatagcaa aatgctaatg
    40501 acatgccatt ctcaacataa atattaaaat ttgaaaaatt aaataaatct gttttaagtc
    40561 ttagtccaac aggcactgct ttataagaaa agataggaat tgaggaatca actttcccag
    40621 aagatttttg acttatacca acattcattt ttaatgataa ggtttcattt aataaatatt
    40681 ccaccttaat tccagattca aagctattaa aatcattatt tttttgaaaa atatgtgaag
    40741 attcttctag aaaatttacc ctttgattca gtgcgttata atatacccct attttccaat
    40801 tttgagcata gccaattaac ccacagaaag taattataaa aagtaaaact atttttttca
    40861 taatattttg aatttttatt ataaataatt tactaaactc actaaaattc aacgaagcac
    40921 ttttaaaact tcatttaatt aaaaacaata tcccaatccc agtccaatat ttatacaaga
    40981 accattgcta gaaattgtgt aaaaattttg tccaccacca acttcgttaa ttaaaatgct
    41041 attattctcc caaactttat taattccaga ataataattt gcaaaaatat ctatttcaaa
    41101 tttagattta attaaatagg atattccaat tccattaaca aaaccaaaag accagttact
    41161 tgtgaaacta taatctgaaa tgctaccttt tatagtgctt gcagaagttt caataactct
    41221 gttataattg taagaatgac aaaaggctaa tgacaaacca ttttcaataa agacgctaaa
    41281 attgttgatt tttagcacat ttatttttga aacaacacta aaagggaaat cattgtaatg
    41341 aaatgatgaa atgaaaaaat tgggaaataa taaatgattt tgactataac ctacactagt
    41401 ttttaatcga aatgtttcat taaatttata atcaaaaaga agtccaaatt cccaactgaa
    41461 aaacattgct ttttctggtt ttaaaaattc agatttttct aaataattag ctgtttgatt
    41521 gcttacatta aaatatgtac caactttaaa gttttgagaa aaaactataa aactgatttg
    41581 gcataatgca aaaagtaaaa ctattttttt tacaatataa gatttaacca ttattataat
    41641 tacgtatttt tacaaataat gtttcatttt gaagttttaa aaactaaaaa ctgaaaaaac
    41701 aattaaaaat tgcgactttc gacttttaac ttttaacttt taactgaatt aattattaca
    41761 atttcttaat tatttctatt tttacaccaa attaaagatt tgacattatg aaatcagctt
    41821 ttttctcaaa aaatttacct cattttattg cagtagcagc atttttgata attgcttcaa
    41881 tttacagcag tccgtatttg aatggaaaac gaatatcgac acacgactat aacacctaca
    41941 cggctattgc aaaagctaat aatgattacg aaaaagaaca caatagatta gtattttgga
    42001 ataatagtat gtttagcggt atgccaactt atgctgtttc tactgccaaa caagaaaata
    42061 tatttttcaa aatttataaa gttttaattt ttaatgatgc cacaccaata aatatgattt
    42121 tctggtattt gcttggattt tatattttat taaacattct tagagtcaat ccgtggttgt
    42181 cgatgttggg agcattaatg tttggatttt catcttatta ttttataatt attgctgctg
    42241 gacatttcac aaaagcaatg gctataggat ttatgggtcc aatacttggt ggagtgcttt
    42301 tagcttttga gcgaaaaaaa ccgtgggcag gaatgttttt aatgactttc tttttagctc
    42361 tgcaaatatt aactaaccac gttcaaataa catactacac tggtttagct gtaattattt
    42421 acggaatttt tgaactcgtt tgggctataa aagaaaaata tttaccacga ttttttaaaa
    42481 ctgttggaat actagcaata ggttttgttt tatcgcttgg aattaatgca gcatttttaa
    42541 tgaccaccca agagtatatt ccctactcta ttcgaggaaa atctgaacta gcaactaagc
    42601 aaggtgcagg ctatacttct ggacttgaca aagactatgc tacaagttgg agctatggaa
    42661 ttggagaaac tttcacttta ttaattccaa atgctaaagg tggtgcttca ttaattccaa
    42721 atgctaaagg tggtgcttca tactctgcat tgtcagaaga ttctgaaacc catgcagtaa
    42781 tcaaaaatgt ttttggaaaa caaaatgcaa atgaaatttg taaaaatatg ccaacctatt
    42841 tcggcgacca agcctttact gccggtcctg tatatgttgg tgcaatagtt gtgtttttgt
    42901 ttgtttttgg actgttaata gtaagaggaa aactaaaatg gtggcttcta acactaacta
    42961 tattctcaat tgtattagct tggggaagaa attttgagtg gcttactcat ttcttcttag
    43021 actatgtacc tggttacaac aagtttagaa ctgtttctat gatattagtg attgccgaaa
    43081 cagctatgcc acttttagct attttatcaa ttgccgaaat tgtaaaaaac aaaattgaca
    43141 aacgcaaact tatgtcgagt ttttatattg cacttggtgt aacagcattt gtaactctca
    43201 ttttcatcat aagtcctagc atcactggtt tgagtggaga aaacaaaagc gaagttagaa
    43261 cagcagaaat gtttgcctca tattttccaa acgaagcaga atatgccaat gcaaaagaac
    43321 agttcaaaaa tgattttata aatgccatta ctcaagacag agccgattta gttagaaaag
    43381 atgctactcg ttcacttgtg tttattttgc ttgcagcagt tttggtttat ttattaataa
    43441 ataaaaaact aaaaccaaac cttgtaattg catcgctagc agtattggtt ttagcagata
    43501 tgtggacaat aaacaaaaga tatttgaacg atgataattt tgttacaaag cgaaaatacg
    43561 aagtaccttt tgacaaaact cctgcagata gttttatcct agcagacaac gacccacatt
    43621 atagagtttg caatctttct actggagcaa ctagcgtgtt taatgacggc tcaacttcgt
    43681 attatcataa atctataggt ggttattctg gagctaaaat gcgcagatac caagaagttc
    43741 acgattctgt gctatttaga gaaatgtatt tctcagaata tattattaga gaaggaatta
    43801 aaaaccaatt tgaaccagca atacttcaac aattgtttga ccaacaagca cgtactccaa
    43861 ttatggacat gctaaataca aaatacatta tctacagccc tgaaggaaaa cctattataa
    43921 ataaaaacgc tttaggaaat gcttggttcg tggatagcat aattaaagtt gaaagtccag
    43981 acgaagaaat tacaaaatta aattcatttt cgcctaaaaa agaagcaata gtagagcaaa
    44041 gatataatga gtttattaaa gattggaaaa atattgaaga cagcacagct acaataacac
    44101 ttaaagatgt tgctcctgac tatgtggttt atgaaagcaa aacaaattct gaacaacttg
    44161 cagtattttc tgaaatttat tacccacacg gttggaaagt tactattgac ggcaacgaaa
    44221 cttcgcactt tagagcaaat tatattttaa gagcaatgct agttcctgct ggtaaacacg
    44281 aaataaaatt tgaatttata cccgaagttt ataaaaaagg agttatgatt tcttacgcta
    44341 gctcaataat tctattttta atgatgggaa ttggattatt ttttgaaaac aaaaagaaaa
    44401 aacaagctaa attaaactct taacttatca aaaaagatat tttgttcaga taattagttt
    44461 attttttaca aatattttta tttgttatta tatttcacat atttttatta aatttgtgtt
    44521 atcaaattca gacaaatgat aaataaaatt gtaattcttg gctctggcaa tttagctttt
    44581 catttttgca aagcagcttg catagctggg gtggaaattt ctcaaatttt tagtcgcaac
    44641 ttagaaactg catcgcaact agcaaatatg gctggctgcg attttacaaa cgatataaat
    44701 aaaattgatg aaaatgcaga cacttttcta tttgcaatga cagacgaagc aaatttaagt
    44761 attgtaaaaa aacttaaacc atatcccgat aaaactctgc tacatactgc cggtagttta
    44821 agtatgaata tttttgaaaa tataactgaa aattacggag tgttttatcc ttttcaaact
    44881 ttttcgagaa attcaaaaat agatttttca aaacttccac tttgtatcga agcttcaaac
    44941 aaacaaactt taaacgcatt aatacaatta agcgaaagac ttagatgcaa ttattatata
    45001 atcaacgaag agcaaagaca agtaattcat ctttctgggg tttttggctg taattttatg
    45061 aaccactgca taagcttagc cgaaaaaatt ttagaagaca ataaaattga taaaaatatt
    45121 attaaagctc tgctaaaaca aagttttgaa aaagtaattg aaaatggagc ttttgcttca
    45181 caaactggtc ctgctgtcag acgagattat gaaattatgg aaaaacatgc ccaattttta
    45241 agtaaagatg agcttttgtt agaaatttat aagttaatga gtaaaagtat tgaagaaaca
    45301 cattttaaaa aatgataaac agtttaaaaa atcttaattt taaagaaaaa ttaaaaaatt
    45361 tagacacttt aattttcgat attgatggag tattatctgc ttcaaatgtt ttaattagcg
    45421 aaacaggcga gcttttacgt tcaacaaatg ttaaagatgg ctatattcta agatatgcta
    45481 ttaaaaaagg tttgcgagta attattttat ctggtggaac taacgaatct gtaaaaattc
    45541 gttacgaagc acttggcatt ccagatgtaa taataaaaag caaaagaaaa attgatgatt
    45601 ttgctaaact aaaagaaaaa tataatctcg acgagggaaa aaccctatat atgggagatg
    45661 atattcctga ttatgagctt ttaaaaactg ttggcttagc ttgttgccct gctgatgcag
    45721 ctgatgaaat aaaagaaatt tcactttatg tttcttcaaa aaaaagtgga gaaggctgtg
    45781 tgagagacat tacagagcaa gtacttagag caaaaggatt atggatggac gatgatgcgt
    45841 ttaacaccta aaaacctaca atatgaacac taacattcta aaactaatcc gctggaaaaa
    45901 tttatttatc attgtactca caatgattgc aatgcgatat gcaattgccc aagcactttt
    45961 tgcttattat aaaattcctc ctgcctttcc tgatattggc tttgtatttc ttgtcctttc
    46021 agcagttttt ttagttatgg ctggtaattt tataaacgat tattttgata gaaaaactga
    46081 ttttataaac aaacccaaga aaactttagt agctttcaaa gttagaagac gacaattaat
    46141 ttctttaaat gtagttttta atgtactagg attaatttgt ggatttattg ctgcttattt
    46201 tgcacataaa ttaactctag gcataatgtt tatttttata gttgctgttt tgtggactta
    46261 ttctgccgag ctaaaaagaa ttttatttat aggcaatttt agcttagctt taataatagc
    46321 tttaattccg ctatcggttg ctcttactga atattttgcc ttagaacact caataacaca
    46381 atgggaagaa aatagcataa gagcaataaa actatctgtt caatttatta ttggcttttc
    46441 aatttttgct tttttattca cttatatccg agaattaata aaagattgca tagaatataa
    46501 tggcgattta gaaacaggaa taaaatcttt tcctacagtt tttggaaaaa ggaaaaccga
    46561 cattattatt gccatattaa gcttagttgc cataattgct attattcttt gttgggaagt
    46621 ttatttgtca aaacttttgt ttttgagaaa tcacataata gcaccgtttt atatttatgc
    46681 ttttttagtg ttcccaacag cattattatt ccttattgca ctaacattga aaacgccgaa
    46741 aaagcataaa actatgctca ctttaattaa tttaataatt gtttttggta ttttatttag
    46801 ctttatattt agctcaatag tttatggaaa aatttaattt tgtaaaaccc aactacacca
    46861 tattacttgc ttctggcagt ccgcgtcgcc aagaacttat ttctaaattt aatttcaatt
    46921 taaaaattgt aaaagcccct gaaaataaag aaattattcc cgataatgtt tttcgcgaaa
    46981 atattgccat tcatcttgca aaagaaaaat cgctaagcta caaagattta aaagaaaacg
    47041 aaattcttgt tactgccgac accatagttt ggaaagacaa taaaatttta gggaagccta
    47101 aagacaaaga agaagctatt gaaatgcttg agtttttgag tggcgatgag cattttgttt
    47161 tcacaggtgt ttgccttcgc tcaataaata aaaccaagag tttttttgca gaaactaaag
    47221 taaagttttc taaactaagc aaagccgaaa ttttatatta tatcgaaaat tataagcctt
    47281 acgacaaagc tggagcatat ggcattcaag agtggattgg catgattgca gttgaatata
    47341 ttgaaggttc attctataat gtaatgggtt tgcccgtgca gaaactttat aaggaaatca
    47401 tggagtttta attgataatt gataattaaa taatttagct tcgctacatt ttctgtcgtc
    47461 tctcggcata gttcaagcaa gcttgactct gcacttgctc cttagaaaat tgataattat
    47521 ttgctcgctt tgctcgcttg ggtttgcttc gcagggttcg caagttcact ctttttagtt
    47581 tttagtgttg agtttttagt ttttagtagt gagttcttaa aaacttcaca atgatagata
    47641 gaatttcttt agtgcagggg ttgtatattc ttgcttcgca gggttcgcaa gctcactctg
    47701 tttgcaatca taacacccat gatgctcgct ccaacaccta tgacgctcgc tcggatttgt
    47761 aatccgagtg tttggaaatg ttacactcgg attgcaaatc cgagcgagcg gggttgtgcg
    47821 gaaaaatccg agcgagcggg atagccaaca gagtttaaaa ttcgtaagtt aaaaaccaaa
    47881 acttcataaa attgaaattt tttactaaat ttgtgcttta attttaaatt ttataaatta
    47941 tttaatctta gaaataatga aaaaactaat tattttaatt ttatcaagca ttttattttt
    48001 ccctgcactt gcagacgagg gaatgtggat tccgatttta atagaaaggt ataatttcga
    48061 cgacatgaaa gccaaaggtt ttaagttaag tgcagaagat atttatagca ttaataaagc
    48121 ttcgatgaaa gatgctattg ttatttttgg cggtggctgt actggcgaac ttatttccga
    48181 ccaaggactt ttaattacaa atcatcactg cggttatgga caaattcaac aacattcgag
    48241 tttagaaaac gactatctaa caaatggttt ttgggctatg tcgcaaaaag atgaacttgc
    48301 aaatcctggc ttaaaagtta cgtttttaat cagtatgagc gaagttacag atgccgtact
    48361 cgaaggtgta aatgataata tgtcagaaca agaaagatat tcaataatta aaaaaaatat
    48421 tgataaaatt aaaaaagctt ctgttgaagg tagccattac gaagcaagag ttgagcagtt
    48481 ttataataac aaccaatatt ttctttttat aaacgaaatt tttacagacg ttagattggt
    48541 tggcgcacca ccttcagcaa ttggaaagtt tggtggcgat accgacaact ggatgtggcc
    48601 ccgccatact ggtgatttcg ctctttttag aatttattgc gataaagatg gcaaacctgc
    48661 cccttattct gaagataatg tgccttataa accaaaaaag tttttcccaa tttcaacaaa
    48721 aggctataaa caaggcgact ttactatggt gtttggatat cccggcacta caacagaata
    48781 tttgccctca tcggcagttg agcaagttat gaacgaaacc gacccttcaa gaataatgat
    48841 tagaaagaaa aaacttgaca tcatcaacgc tgccatgaac tcagacccac taattagaat
    48901 tcaatattct gcaaaagctg cttctattgc taatggttgg aaaaaatgga ttggcgaaat
    48961 caacgggctt aagcgcttag acgcagtaaa tgttaaaaaa caatacgaac aagattttat
    49021 gaattgggta aatgctgata gcgaaagaac aaaaaagtac aaatacattt taagccaata
    49081 caaaactatt tacgaaaatt tatctccata taatttagca atgatttata taaacgaagc
    49141 aggttttggt agcgatgcag ttttattggc tttgagaatg agaagtttag aaggtgttgt
    49201 tggcaaatct gctgaaagca ttgaagttct taaaaaagat gcacaaacaa gagcagacaa
    49261 tcattttaaa aattataacg aagaaacaga caaaaaaatg tttgctgaaa tgcttaaact
    49321 ttattacgaa aatattccac aagaatttca tccaaattac tttgctcaac ttgataaaat
    49381 taaagtaaaa actggaaaca aatatgaagc tattgctgaa cattgctaca aaacttcaat
    49441 ttttggaaat caagaaaagt tcaacaactt tttaaattcg ttttcagaaa aatcctacaa
    49501 aactttaatg aaagacccta tttatctatt aatgaaagat tatgttgata tttatatcaa
    49561 taaagttgat gcagaatttt ctcgttatag cacacaatta gacagccttg atagaattta
    49621 tatgaaagca caaatcgaac atcaagatga taaagttttg ttccctgatg ctaacttcac
    49681 tttgagaatt acttacggaa aaatagatag ctactatcca gctgacggtg ttaaatacaa
    49741 ctacttcact actttagaag gaattatgga aaaagataat cctgaagttt atgattatga
    49801 tgttccgcaa aaacttagag atttgtacga agcaaaagac tatggaagat atgcagatgc
    49861 tgatggtagc ttacatattt gttttaccgc ttctaaccat acaactggtg gaaattcagg
    49921 ctcgcctgta attaatggaa acggagaatt gattggaata aacttcgaca gaaactggga
    49981 aggcacaatg agcgacatta tgtacgatcc agaaatgtgt agaaatattt ctatcgacat
    50041 tcgctatgct ttatttataa ttgataaata cgccggtgca actcatttgc taaaagaaat
    50101 ggatattaga taaaaatgaa agtcggtttt gtaagcatat tattactgat ttttgcaaca
    50161 tttggaaatg taaattctca aaatttatat tatcattttt atgatgatgt tcaagttttt
    50221 acagactcta catcattatt aaatgctatt ttcttagatg atgtatataa acatgtttac
    50281 aaaaacgaca gtatttcttt ttggattatt gaagcagaca cttgtgatgc aagtgatgtg
    50341 tctgggattc cttattgcca atcttgtagt gttggtgcta atttaatttt acaaaattct
    50401 tataagtttt ctattattga cgaaaaactt gttgttggca catgctggga ttatttgaac
    50461 gcaatatata gaagtgttga atcgtctgat ttatttagga aaactcaagt ttttaattcc
    50521 aaaaaatctg gtccttatgc tccaaaagaa atgcttaaac ctggtgattg ggtttatcac
    50581 ataaatagac aatttagaaa cattgaacat tctgcaattt ttgtagcctg gaaagattat
    50641 gaaaaaggaa ttgctatcac aatgtcttac atgggtttag gacgagcaag accagcaaga
    50701 tatggcgaat acgatttgaa ctctgtatat gcgattttta gaattgaaga tgaattttgg
    50761 taaaattaaa actctactaa tttgttgaag tagtgtattt tataggtatt ccaattttta
    50821 ttcctatgcc tgcacaatat cctctatgtt taaaaatata agttgaattt tcttttaaaa
    50881 tttgatttat gccatgatcg tattttgctt gtataaagac taacaaattt ggcttaattt
    50941 tatactcaaa accacaacct acaactaaac taaaatttaa aggtttatat gtgtctgtgt
    51001 tcacaacgtt tacttgctca accgacactc cttcaaaagt tttaggtaat tttgcataag
    51061 tttttgcttg taataaaaac tcagcattta cgcctaagtc tataaataaa ttaagttttt
    51121 tgccaccaaa taaattataa gttaataaaa gtggaaaaga aagatattga aatttattaa
    51181 aggttttaaa ctctcccatt ggtgtgtttt cagcatttac ataacttgca aaataataat
    51241 tccctttact catcaaagaa acgccaaatt tgaaagataa ttccgaatga aagcgatagt
    51301 caaagtctat tccataagat aaatagttag aagaaaatga ttgtggaaat cctaaaattt
    51361 tataatgtga aagtcctacc catgggttta caaataaagt tgaatccgtc cttacttccc
    51421 aagcttttag actgtaagaa gataagacaa gcaataaaat taaaatatat ttacttcttt
    51481 ttatttttgg taacattact aagaatttta tttgaaaaag ttatggtttc aagctcatct
    51541 cctaccatta tatttgtgtt ttcatcaata atttttccat taattttaga aatagaaaaa
    51601 cctcgtttca aaacattttc aggattagct aattctatat tttttgataa attttgaagt
    51661 tttaactcat tttttgaaag caaaatttct gtatttttct tcaaacttga aaatgaattt
    51721 tctaaatgtt tatttgccaa ataagttttt tgatgagtta agagttttaa attttcaaaa
    51781 taattattta aacgattttc atttttacga attaattgaa cagaagcatt ttttactcta
    51841 tacatatttc tgttgcagtc ttcgttattt ttaattaaaa catctttgat tttatatatt
    51901 ttcaaaccca atgaagccaa atacatttct ttatttttga taacatattg agtttcagtc
    51961 actatagatt tgaaaatttc aaaaacttct gtttcaaaaa cattattata gtcaacaata
    52021 aaattggcta ctgctgttgg tgttttaagt tttgtatgag ccaccatatc ggtaacagat
    52081 tcgtctcttt cgtgtccaat tcctgctata acagggatag gaaattgagt tacatgataa
    52141 gcaatattat aattgtcgaa atagctcaaa tcgcttttag aaccaccacc tctaataatt
    52201 gctacaacat caaaattttc tatattttca taaatttcgt ttaaggcttc aataacagat
    52261 tcttctgttc ctgtaccttg catatttgcc tcaaacaagc taagttcaaa tttatatcca
    52321 aatttattgt tttctatgtg atttataaaa tcttcaaagc ctgctgccga actcgaagaa
    52381 attattgcaa ctctttgtat tacatcagga aggtgtaaaa gtttattcat atcaaatacg
    52441 ccttcgctta taagtttatc aataatttct ttttttcgtt tttctaaatc tcccaaagta
    52501 aacgatggtt ctatttctct gatattcaaa gaaatgctat aaagttcgtg aaattcaaca
    52561 cttactttaa ccaaaatgct cattcctttt tcaatatctc tttgggtaac agacttaaag
    52621 tatgcagcaa taaatctata ttgcgaagcc caaattgtag cacgcatttg agcaataatt
    52681 ttatcatcat attcggattt ttgaaccaac tccaaataac aatgtccact tctatgaaga
    52741 tttatattat ttatttctgc aacaagccaa actggggcat caaaactttc gttaattgcc
    52801 gattttattt tttcgtttaa ttctaataat gtcaggcttt gtcgagacat tttactgttt
    52861 tattaatttt gcagaatatt tttttgaatt aatgcttact gtaacaatat aaatacttgg
    52921 ttttaagttt gccaaatcag gaatatttac tgtttgatta ttttcagaag ctcttaattt
    52981 aaaccacaaa taatgttttc tacaaagtaa atcgcttact tcaatcacaa tttcatctcc
    53041 aatattatta tctaaatgaa tgttaaaatc gtgataataa ggatttggac ttagagtaag
    53101 tttttccatt ttggaaattt ctttttctac agttaaaatt tgtgaagcta agtcaaaatc
    53161 ggggattcca taacctaaat aagcatttgg attgttatat aaatttgcag tttgctttgc
    53221 tatttccata atttcaaaat ttgttttttc tggaaattgt tgccacaaac aagcaaacat
    53281 acctgccaaa agtggtgcag aaaaagaagt tccgttacca taaccaaacc ctccatttgg
    53341 ttcttgatta aaagtacctg ctcccatagc tactaaatct ggttttattc tgccatcata
    53401 acttggacct atagagctaa atgctgcata agttgaatct gcataaacag ctccagcagt
    53461 aataatatct tttgcatctc ctggtgaagc aatccatgga tgtgactgac tttctcctga
    53521 atttgcagca ctaacgacaa gtaacattcc tttatgtgag gcttcgcgag cagctaaagc
    53581 aattattgta gtttcgccgt ccatatcttc atagctatga ttatttactg gcatatcaaa
    53641 agttaaatag cctaacgaaa cattaacgat gtctgtacca atgctatcag caaattcagc
    53701 tgcagaaatc caatttattt cctcaataag attttctgta attggatctt ctgacattaa
    53761 aagaacatat tcagcctctg gtgcagagcc tacaagataa tatggagaat ttcctgcaag
    53821 aattgaaaac acacgcattc catgagtgct ataattaaat acagaatctc caccgtgtac
    53881 gaaatttcga gttgctatca ctctattgtc ttggaataca tgttgcaaac ttggaacgtc
    53941 aagcgttgcg gtaaatcctg catcaataac tgcaatcaac attccttgac cacgataacc
    54001 tttattatgc aacttttgtc cattgtgcat aaaaatttga ttttgactag gaccatactt
    54061 ataataaaaa tcgccttctt cgcctttttg tatatctcgc aaaactttat actcattacc
    54121 aagcacacgc tcatcttcgg tttttatctc ataggttatt gaaccactaa caaaacttaa
    54181 atttttaata agctcgatag gttcaggatt ttctgaataa actaaaacag aattaaacca
    54241 tttcgataca tttaaaattt caacaccaca atttcttatg ctatctatat agaactgact
    54301 tacaggcaaa tcagaataat taatcataat attttgattt gctcttcttt gcaaagctct
    54361 aaagcttaaa aattcttcag gcgaatctaa actataaaga ttgtgatttt tgtcggtcaa
    54421 ttcaaaataa tacttattag gtgctacttc aagttgagca aaaatattta aacttaaaaa
    54481 tgtaaaaaac actaaaaatt ttattttcat aaaaaaaatt ttaattaaga attaatcttc
    54541 cctcacaaac tcttgaataa aaattcttcc cacagtaatt ctttcttcta taggcacgtt
    54601 atgagttact tcttgagagt ttatatatat ttctttttta taaacaagtc cgatattttt
    54661 ggcatatatt tcataagcca aatctttatg aattagcgac acactagagt cttgcataac
    54721 cgtaagacat gaatcaaact gaacagaacc aatattaaat ttatggtcaa atcttaaaat
    54781 ttcgtattct tgaacttttt ggttgtttaa taaatttcca ttccaagaca gattttcttt
    54841 tattggaaaa cgaattttta tttttctttg attttcttcg actttcacag catatttatt
    54901 tgtgagtttt gccgaccaca ccgaattgat aatccaacta taattttcat cttttctagt
    54961 atatctttct attcgccaag ccgtatcgtt ttcgttatca ataaaaggcg attctacaat
    55021 ttcgttcaaa taataaatgc ttgtatcata aacatttgaa ggcttatcaa tattaatttc
    55081 tgtaacttta taaataatac tattcccttc aattaatgga aaatattctt gccctaatgg
    55141 aaattttcga ggtttaatat tttctttatt gcaagcaacc aaaccaagac ttaatattaa
    55201 taatatgaaa attcgtgttt tcaatttaaa gcaaaattaa ttaatttttg caataattcc
    55261 accacaaacg aggtcattat tttcataaat aactgccgat tgccctggag taaccgcaga
    55321 tacgggttgt ttgaaattaa ttttaatcat ttcgccttcc attatgaaac tacaatcttc
    55381 gccttcgtta ttatatctaa ttttacaatt accttgcatt tcgcccttta tttcttcata
    55441 tttcattaag tttacatctt ttatccaaac tgttgtttgc aaaagttctt ctcgttttcc
    55501 aagagtaatt gtgtttgttt tagcatcaat atttgtaaca taaactggat atcccatagc
    55561 aatttccaaa ccttttctct gtccaatagt ataaaatggg aatcctttat gtttacccaa
    55621 aatatttcca ttagtatcta taaaattccc ttgacctgga ttatcattaa tttctgggat
    55681 tctttctcgc aagaattgtc tatagtcatt gtctggaata aaacaaattt cttggctttc
    55741 tttcttattt gctattttct cgcattgatg tttatgtgca atttctctaa cctctgattt
    55801 taataaacta cctaaaggaa aaattgtttg agcaagattt tcttggctaa gagtccacaa
    55861 aaaataagtt tggtctttat ttgtatcttt ggctttacgc aaaaaatatc taccattttc
    55921 atttacaatt tgagcgtaat gtcctgttgc ataatattcg caatttaaat ttttcattaa
    55981 atctaacatt ttgccccatt ttactgtttt attgcatagt acacaagggt ttggcgtaaa
    56041 gcctgccata tattctgata taaaattagt tatcacaatt ttatcgaaat attctcttat
    56101 atcgagtatt tggtgaggaa aaccaagttt ttttgccaaa ttttcggctt ctacaaatga
    56161 atcaaaatta tctgcacaag attttaaaat attatcgtaa gcatgaaaag taaccccctg
    56221 aacatcaaaa ccttgctctt ttagcaacaa agctactaca gaagaatctg tccctccact
    56281 catagctaat aatatttttt tctttgttgc cattttttga aacaacaatt acgatttatt
    56341 aatgtttaca agttaaaaat aagattttta atgggcaata accttaaact caactcgtct
    56401 attcatttgt ctgccttcgt cagtttcgtt tgtagctata ggtttttcga atgcgtaacc
    56461 ttcataagtt aatctatttt tatcaacacc tttttctata agataatcaa caacagattg
    56521 agctctttct ctcgataatt ttttattata ttcaagcgag cctctatcat cggtatgtcc
    56581 agaaatttca atttttaatt ttggttggtc attcaaaata gctaaaactc tatttagttc
    56641 agcaaacgat tcaggacgca aagttgctct atcgtaatca aagaaaatat tttttagaac
    56701 tactgtaatt ccgacttcta ttctttgcaa atttacatca ataactgctt cattatattc
    56761 ggcggtatca gcaatattaa agttttctga atggaataaa tatccttctt tactaacatt
    56821 tagtccgtaa tttttaccag aaggcaaatt aattagatac ccacctgttt gtgagttggt
    56881 tataaattca gcaataactt catctatact attatctata atttcgattt tagcctctaa
    56941 agggaaagtg gttgtagcat ctctaactac tccttttaca agtgtaacta gaaccttatc
    57001 ttcttctttt tgagtttctt tatcaacatg atattttagt tcgtacaaat ctagcaatcc
    57061 ataaccttcg ggacgtaccg aagcataata agctgttgtt ccgcttgcag ttgttacata
    57121 aaatgcatca tctaatggtg tattaatagg ataaccaaga tttactggtg ttgtccattc
    57181 tccatctgca tttttaacag ttttaaaaat atctaaacct cccatcgagt tatgaccttt
    57241 agaacaaaaa tacattgttc taccatctgg gtgaaggaaa aaatcgactt catcatatgg
    57301 agtgtttact actggaccga gatttactgg tgatgaccaa ttcccatttt tatctaaggt
    57361 agcgtaaaat atatctcttc ctccaatcga agtagcaggg ttgtccgaaa taaaatacaa
    57421 agttttgtta tcataagaaa ttccagccat agattcgtga tacttagtat ttacattacc
    57481 ttttaatggt gttggttttg accatctact tcctttaagt ttagtttcgt aaatatctcc
    57541 accattagta tccatataaa tatacatagt ttgtccgtct ggagaaatac caacaacggc
    57601 atcatgtagt ttggtattta aaggcgaacc tacagatctt gcttttgtcc agccatcttc
    57661 agttttatta ctttcatata tatcttcaaa aaacattcca tctggtgcta cttgtcctcc
    57721 tgtagagcct tttcggcgag aagtaaaata cagttttgac ccgtctgcaa ctaccatagg
    57781 tccatattca cgatattctg tatttatatt ttctcccaag tttatgattt ctactttgac
    57841 agtatctttt accaattctt ttccatttct acattcggca atatgctttc caacagcttc
    57901 taactctact ttatctttta catcttgtaa ataactagaa tacaatgaaa tggctttatc
    57961 aaaatcataa ttaaaatgat agcctcgagc aagataaaac tcaatttttt tatctacttt
    58021 aggatttaat tcatatgctt ttttaaaata ttctaaacaa tcgtactttt gagaagaatt
    58081 ataataacaa tatcctattt taaaattcaa atctgaattg tttggattaa attcatttgc
    58141 tttaagatat aatttaagtc cctcttctat tttattttct gaatacacat aaatatcagc
    58201 attttttatt tgagaccttg ctacttttaa ttgttctttt ttattaggaa agtttgctgg
    58261 tgtaaacttt acatcaactt gagaatagga taaaacacta attacacaaa aaattaatat
    58321 tactaatatt tgtctcatta ttaacgattt attttataga tataacttta aattcaactc
    58381 ttctattttc ttgacgtcct tcttcggtat cgttagttgc aataggttgt aaatacgcat
    58441 atcctttata agttagtctt gatttgctaa ttcctttgtc aactaaataa tcaactacag
    58501 attttgctct agcttctgat aggtcggtat tgaattttag acttccgtga ttatctgtat
    58561 gtccacctat ttctatggtc atacttggat atgaattaag caatgctact aatcggtcta
    58621 attcaggtaa agaagttgga cgcaaagttt cttttccata atcgaagaaa atatttttaa
    58681 gtactatttt cgagcctatt ccgactttac tcaaaacaat atctttagta acttcttgat
    58741 aatctgtagc atcgggaata ttaatatttt cgctatagaa caaatagtct tgtgctttaa
    58801 ctgctatacc ataatctttt cctgaaggca agcttactag atatttacct gttgctgcat
    58861 tagaagaaat tgtagaaata agttcatttt tctcattatc aacaatttct atacttgcct
    58921 cgactggtgc agtagagata gcatctgtaa ttgttccttt cataattgtt agtcttactt
    58981 ttttaacttc tactacttct tgttttactt tttgtgagat tgttgcatta gagctagcta
    59041 ttaaaatgtc ttctgctccc ataaccatca ttttttcagg acctagaaaa gtaattttat
    59101 aaatatcttg caatccatat cctccttcac gcgagcttga atagtaagca tagcgagcat
    59161 ttcctgcaac agtaaagaaa acatcgtctt cgggtgtgtt aactgggtat cctatattca
    59221 ctggctctgt ccatttgccc atatcatctt tttcgctata ataaatatca tagcctccca
    59281 ttcctccttc gcgtttagaa gaaaagtaca gagttctacc atcgggatgt gaaaacactc
    59341 cttcttcgtc atattttgaa tttattggtg ctccaatatt tttagaaggt ccccaatttc
    59401 cattaggttg caattcgctt acataaatat ctttacctcc ataagaggaa tttccaaaat
    59461 catcttttgg tttatcacta acaaaataaa gtctttttcc atcataagag atagaagcag
    59521 aagtttcatg atatttagta ttaatatttt tgtttaattc ttttggtttt gaccaagtgt
    59581 ttcctcttaa tttactttca aaaatggttc catctggcac tcccttgtaa gtgtataaag
    59641 tttgtccatc tggcgacaaa ccaactgttg catcatgccc gtcggtattc aaatctctca
    59701 tatttactgc ttgcgaccaa cctttttcat cattataaga aacatataaa tcttcataat
    59761 atatcatgtc gtttgggtct aattctccac cagtactacc ttcccttcta gaagtaaaaa
    59821 ttaaaactga ttgatcggca gtaattacag gtccataatc aggatatttt gaattaatgg
    59881 caagtccgag gttatcaata aaaactctaa ttggattttt gattaatttt ttacctgatt
    59941 cacattcttc aattcttttg tcaacatcat attctggtcc atcttttttc ttagattttt
    60001 ttgaaacaac accagaatat tcatgtttaa aactcctata actagttaaa gcttggtcaa
    60061 aatcataatt atattgataa gccataccca ataaataaca aacatcaact gctacatttg
    60121 gttttgcttt ataagctttt tctaaaaaag gcaaagctct tgttttttct gttgaatata
    60181 aataacaagc acctatttta taatttaatt ctggagaatg tggattatat tgatttgctt
    60241 ttaaatactc tggcaaagct gctcgataag ctcctgattg tcctaagcta taataatatt
    60301 ctgcaacttt tagagccttc atagcttcct tataaccatc tttagattgc ttaaattctt
    60361 ttttattaat ttctacttct acttgagagt atgtttgaac tcctatcgca aaaagtattg
    60421 aaaatataat taaaaattta ctcgttttca tttttctaaa ttttaaaatt aaaattgact
    60481 aatattcttt gcatactgaa caatattctt ttgccacttc aagcccaaga tctctagctt
    60541 ttttccaatc agcacatgca tcttccattt gccttaacat ttcataagcg ctacctctat
    60601 gaatataagc ataagcataa gaaggatcaa aaaagatagc tttattatat gcttctatcg
    60661 cagatttata atctttatct ttatagtaac ataagcccat attattatat gcataaacat
    60721 aatctttttt tagctttaac acattattaa aacattctat tgcttcttga tattttcctt
    60781 gatcgtataa agtaattcct ttattattga tagctaaaaa ataatctcta tcaattctta
    60841 atgcgtggtc aaatgcttca atagctccag cataatcttt attcaaacgc tttacacttc
    60901 ccaaattatt atataacaga ggattgtatg cgtctaactc aatagcttta ctataatcag
    60961 aaatagctgc atcatagttt tccaacattc tatttgcaga agctctgtca ttgtatgaaa
    61021 acacgtgctt agcatcaatt tttattgcct cattataaaa ttctattgct tcagaatatt
    61081 tttcttgatt aaatttaatc acaccaagga aataataaag gtttacatct ttatgattta
    61141 atgacttagc ttttagaaag tttttttcag catcttctaa tttgtctaaa taatattgag
    61201 aatatgctaa taaataagct gcttctccgc tgttttgttt tttcgcaaag aatttttcaa
    61261 aatcagattc tgcactttta aaattagata attgcacata actagaacct ctacctttat
    61321 atgcgtcaat taaattagga ttcatctgta aagcttttga ataactttgt atagctgcct
    61381 gatactgacc ttgttgatat aattgtactg cgtcattaaa tgcaacattt gcttcttggt
    61441 catcagcaag gaccaaagtg cttgtagtat cagatatggc tgtgcgatca ctatcatatc
    61501 ctattttgat aggaataata atttgcgaag tatcaatttc ttgagccaat gcaaaaaatg
    61561 gaataataga aataacaaaa attaaaattt gtttcttcat aactcaacaa aattacagtt
    61621 tatactttaa aattatagca aatatataaa aattttcttg tatataaaaa attaatcagc
    61681 gtttattcca tgacagtgtt tatatttttt accacttcca catggacaag gctcatttcg
    61741 tcctactttt ttctccactc ttactgggtc tgcaacagat tttcctttac ttgggtcgtg
    61801 ataaccagga tcttcacgtc ctgttttcat tttactcata tctagcttag atcttactcg
    61861 agcctcccta acctcatcag cttcttgaac aggtataaaa cctctcatca aactataaac
    61921 tactgattta ttaaccttat ctagcattgc tttaaacaat tcgaaagatt ctaacttata
    61981 aatcaataat gggtcttttt gttcgtaaga agccgactgt accgattgtc ttaaatcgtc
    62041 catttctctc aaatgttctt tccaatattc gtcaatagtt tttaaaacaa tggttttatc
    62101 gtaagcgtta acaatttctt cgcctttatt attgtatgtt ttttccaaat ttacaataac
    62161 tggatattct tttttgccgt ccgaaattgg cactaagata ttgttatata ttgattgttt
    62221 attttcaaaa actgttttaa taataggata agcttgatcg gctatagctt gagctttacg
    62281 tttgtgattt tctcttgctt ttccatagat ataatctata aagtcatcgt atttaaggtt
    62341 ttcatattcc tgttcgctta aatcaagttc tattgaaagc tttctaaata gttctaattt
    62401 aacttcttcg tattcataat tttcaaaata ctcatcagcc aaacttacgc acacatcata
    62461 tatcatatta gcaatatctg ctccaagtct ttcgccgtct aatgcatttc tacgtctttt
    62521 ataaataact gtacgttgcg agttcatcac atcgtcgtat tccaaaagtc ttttacgtat
    62581 tccgaagtta ttttcttcta cttttttctg agctctttct attgacttag aaatcattga
    62641 atgttgaatt acttctcctt ctttcaatcc cattctatcc atcattctag cgattctttc
    62701 cgagccgaac aaacgcatca agtcatcttc taaagacaca aagaattgcg aagaacctgg
    62761 gtctccctga cgaccagcac gtcctctcaa ctgacggtca acacgacgag actcatgtcg
    62821 ttctgtaccg ataattgcca aacctcctgc ttctttaact ttatcgctta gtttaatgtc
    62881 ggttccacga ccagccatat ttgttgcaat agtaacattt gaagaataac cagcttctgc
    62941 aactatatca gcctcgcgtt tatgaagttt agcatttaaa acattatgct ttatcccttt
    63001 tcgagtaaga attttgctta aaagttcaga tatttctaca gaagttgtcc ctacaagaac
    63061 tggacgtcct tcttttatta ttctttcaat ttcgttaatt actgctccat atttttcgcg
    63121 atttgtttta taaaccaaat cttctctatc aatacgaata attggtctat ttgttggaat
    63181 tactacaaca tctaacttat aaatatccca gaactctcca gcttcggttt cggcagtacc
    63241 agtcattccc gaaagtttgt gatacattct aaagtaattc tgcaaagtaa ttgttgcata
    63301 agtttgagtt gctgcttcaa ttttaacgtt ttctttagct tctattgctt ggtgcaagcc
    63361 gtctgaataa cgtcttcctt ccattatacg tcctgtttgt tcgtcaacaa tcttaacttt
    63421 attgtctatc acaacatatt caacatcttt ttcaaataaa gtataagctt ttaaaagttg
    63481 acttacagta tggatacgct ctgattttat agaaaaatcg ctaataattg cgtctttttt
    63541 gtttaatctt tcgttttctg gcaatgtact ttttgataat tctgataatt ctgttgctat
    63601 atcgggcaaa acaaaaaagt taggatcaga agttgcatgt gccaataggt caaaaccctt
    63661 gtcggtaagt tctatagagt tttgcttttc atcaattaca aaatacaaat cttcggtaac
    63721 taaatgcatt tgtctgttat tgtctgccat gtaaaagttt tcggtttttt gcaaaagggt
    63781 tttcattcct tgctcactca aaaacttaat taatggttta tgttttggta aagctttaaa
    63841 tgcttgaagc aatttgaaac ctccatcttt ttcattgtcg gcagctataa gttttttagc
    63901 ttcgttaaag atttcgttaa ataaatcacg ttgagcattt actaattttt gcacatgagg
    63961 tttaagttcg tcaaataatt ggtcttcgcc ttttggtgtt ggtcccgaaa taataagcgg
    64021 agtacgtgca tcatcaatca aaacagagtc aacctcgtca actattgcat aattgtgtct
    64081 gcgttgaacc aaatctgatg ggtttaaagc catattatca cgcaaatagt cgaaaccaaa
    64141 ttcattatta gttccaaaag taatatctgc catatatgca attctacgag cttcagaatt
    64201 tggttcgtgc ttgtcgatac aatcaaccga caaaccgtgg aacatataaa tcggtcccat
    64261 ccactcggag tcacgttttg caagataatc gttaactgta actacatgca cacctcgtcc
    64321 tgtaagtgca tttaaaaaca ctggaagagt tgctaccaaa gttttacctt ctccagttgc
    64381 catttcggcg atttttccag agtgaagcac tactcctcca ataatttgta catcgtaatg
    64441 caccatatcc caagtaactg tatttccacc agctacccaa gaattaaacc acactgcttt
    64501 atcgccttta atttcaacga aatcgttgtg agttgctaaa tttttatcga attcgcttgc
    64561 agtaactaca acttctttat tttctttaaa tctacgtgca gtttctttaa ctattgcaaa
    64621 agcttcgggt aaaatttcgt ttaaataact ttcaaggtct ttatctattt tttcctctat
    64681 tttatcaatt tcgttaaaaa tttcttctct ttcccaaata tctaaagact ctgcttttgc
    64741 tttaagctct gtaattttgt cttgctcagg ttttatatgt tcggctattt tcttttttaa
    64801 attttccgac cttgcacgta attcatcatt agaatcttta gttatttcag gctctatagc
    64861 caatactttg tccaaaaatg gttgaattgc ttttaaatct ttttctgatt tagagccaaa
    64921 taactttaag ataaaactac caaatcccat atttttcttt atatattttt tataattaat
    64981 ttaacttaca aagttacatt aatttattag agctacaata aaatatgtga aataattgta
    65041 tttttaattt ttaaaaaaac atgtactatt ctgcaagcaa ccaatttagc gaaaaacttg
    65101 taaaaacatt atctgtataa ttatcttttt cgaaaaacaa tcctatatct ccattttgca
    65161 atacacatag cgaagaatat gcagcagaac ccgcataaat agttttagat tttgaccaag
    65221 ttgctccatt gtcaagactg tattttacag ataaattttc tcttttattt ttcgattcca
    65281 aatttgaaaa aattaatatg cttttacttt ctccttctat ttcgtaagaa taatttaaaa
    65341 tacttgaatt gcaacttggg tcaattaaag cagaatcagg ttttgaagtc caagtttttc
    65401 cataatcatc agaaaggtga acatatctat agcccaaacc attaactcta ctgtttatca
    65461 tcaaagtgcc atcagctaat tcaataattt ttgattcgtc tgccggtttt attgcattat
    65521 caataaaata ccaagtttca ccatgattat cagaaccaaa aacacataat ccattttcga
    65581 gatttaccaa agtgtgtaaa agttttcccg aatttgtttg aattccttct cctgaagtta
    65641 tgaatttaaa attattatgc cattctggtt ttgaaatttg ctcagtaata tcaacatgtt
    65701 tagaccaagt ttttccatta tccgcacttt taacataatg caaataataa acttcttttt
    65761 ctaattttaa attcatataa ttgtaaaaca aaaaaatctc gcctgtaatt ttatcaacaa
    65821 ttatcgaagg gtctgaagca gattcgccaa gaggaaaatc aacaatcact tcaatttctg
    65881 accaagtttt tccattatct tcgcttctgc gaatagcgat attaatatct tcgttataac
    65941 gcaaatctcc acaggacaca actctttcgt caattgctgc aattaaatcg ccatttggag
    66001 cttttgcaag agcaggaata cgataacaag aaacaccttc tttcaaattt gtgttaaaca
    66061 aggattgaaa ctctatttgg gaataagatt ttccaaataa aaaaagaaga agtacgacta
    66121 aaaaaactct aatttttaca aaaaaattat tcatcttttt cccagtcaga aagtatgcca
    66181 ttaatttcgt cagttgtttt tctcaagcgt gatttacatt ctttaatcaa aaaagttgct
    66241 cttttaactt tttttgacaa aacatctaaa tctaactcgt ctgtttcgat ttcggctaaa
    66301 atttcttcga gttcgttcat tgcttttgtg tatgaaaatt ctttttcgct catagtttta
    66361 aatttttgat tttattccca ttctattgtt gcaggcggtt ttgatgatat gtcatacacc
    66421 actctattaa cgcctcttac tttattaata atatcgtttg aaacttttgc taaaaattcg
    66481 taaggcaaat gaacccagtc ggcagtcatt ccgtctattg agtttacaga gcgaagtgct
    66541 actacatttt cataagttct ttcgtcaccc attactccaa cagactgcac aggcagtaga
    66601 atcgctcctg cttgccaaac tttatcataa agtccccatt cttttaaact tgaaataaaa
    66661 atatcatcaa cattttgaat aatttttact ttttcttctg ttacttctcc caaaattcta
    66721 attgccaaac caggtccagg aaaaggatga cgacctaaca agctatcaga aattcccaaa
    66781 tcttttccaa caattctaac ctcatcttta aataacaggc gaagtggttc aacaattttc
    66841 aatttcatat aatcaggcaa accaccaaca ttatgatgag attttattgt aacagaagga
    66901 ccttttacag aaactgactc aataacatca ggataaattg taccttgacc caaccaaacc
    66961 acatcttcta ttttatgagc ttcttcatca aatacttcga taaataattt gcctatgatt
    67021 ttgcgttttt gttcagggtc ttttactcca gccaattcct tataaaatct ttgttttgca
    67081 tcaacgccaa tcacgttaag tcccatgcct ttgtacgatt ccaacacttt ttcaaattca
    67141 tttttacgta acaaaccgtt atcaacaaaa acgcatgtaa gttgagaacc tattgccttg
    67201 tttagcaaaa ctccagtaac cgtactatca accccgcccg acaagccaag cacaacttta
    67261 tctttaccta ttttttgttt taattcagaa attgtttcat caataaacga agccgaagtc
    67321 cagtcttttt tacagccaca aatatccaca acaaaatttt ctaaaatttt atttccttct
    67381 aaagaatgag aagtttcggc atgaaattga attccccaag ttgcctcatc agcaatttta
    67441 tatgcagcaa ctttcacatc atttgtagaa gcaattattt tgtaattatc tggaactttc
    67501 acaatagtat cgccatgcga catccaaacc tgagaatgtt ggtcaacgcc tttaaaaagt
    67561 ttatcgcttg agtcaacaaa ttttagattt actcttccat attcgcgagt gtttgaataa
    67621 gcaacttcgc caccataaaa atgagataaa tattgagcgc cgtaacaaat tcccaaaaga
    67681 ggcaatttcc ctttaatatt tttcaaatca ggaattaaag catctttatc tctcaccgaa
    67741 tacggacttc cgcttaaaat cacaccttta attgtagaat cgagttctgg aattttatta
    67801 taaggataaa tttcgcaata aacgcttaaa tctctaagtc tgcgggctat caactgagta
    67861 tattgcgagc caaaatctaa aattaaaatt ttctctgcca ttttcgttat ttttcgtaat
    67921 gcaaatatga tgtttttttt tgaatattag aaattcaaaa agtttttctt acttttgtta
    67981 aattaatatt ttaattttta tgaatttaaa agacataaaa tacgattgca gacattttga
    68041 cggtgctatt ccttgtgtgc caaacaaaaa acatggcgtt gattgcttaa actgcaaata
    68101 ttatgataaa atcggtaaga aaattttaat tattaaactc ggagccatgg gcgatgttat
    68161 tcgcactacg cctttagttg ttaaatatcg cgaactttat cccgattgtc atattacttg
    68221 gttaacgctt tccccagaag ttttgccaaa agataaaatt gacaaaattt taaaatggaa
    68281 tgaaagttca atttttataa ttcaaaataa aaaatacgac attgcaataa accttgataa
    68341 agaagacgag gcttgtattt tattgcaaaa agtagaagct aaagaaaaat atggttttat
    68401 ttggaaagac aatcatatag atgcagccac acaagcagct gaacataaat taattacagg
    68461 attttttgac cattattcac aaaaaaacac aaaacactat ttagacgaaa tttttgaaat
    68521 ctgccatttg cagtttaatg acgagcctta tttattaaat tacaacaaaa atttagctga
    68581 acaatggcaa gcaaaaatca aagaaaaaag caaaggaaaa actattgtag gtttaaatac
    68641 tggtgttgga aatcgttggg caacacgact tttacctgaa gaacattgga tttctttgat
    68701 aaacctactt ttgaaaaatg atttttatcc aattattttg ggcggtcctc aagaaaacga
    68761 aaaaaatagc agatttgcag acaaaactgg tgcatattat cctggcactt tttctttaga
    68821 agaatttttt gctataagct ctgcctgcga aattattgtt acacaagttt cgatgatgat
    68881 gcacattgca actgctttgc aaaagaagat ggttttaatg aataatattt tcaataaaca
    68941 tgaattttat ctttacaata gaggagtaat tgtagagcca gaatctggct gcgattgctt
    69001 ttatggcaat aaatgcaaaa gagaaacttc gtgtatgtac gatgtaaaac ctgaaacttt
    69061 attgaaagaa attttaaaac ttaaaaaaat ataatgcgaa tagcttattt atcaaccttt
    69121 tatccattta gaggcggaat tgcacagttt aatgcttcgc tttttagaga attcgaaaaa
    69181 gcaggtcata ctactcaggc ttttacattt accacacaat atccacaatt tttatttcct
    69241 ggcgaaacac aatttgtaca agaaaatgat attgttgaca aaattgactc tcaaagaatt
    69301 ttaagtactg caaatccatt cacttggtgt tcggctgcaa aaaaaatccg aaaaacaaat
    69361 ccagatttac ttataatgaa atattggatg tcgtattttg gtccgtcttt aggttttgtt
    69421 gctggaaaaa tgaataaaga cactaaagtt cttacaattt tagataatgt tattcctcac
    69481 gaaaaaaagt tttttgattc tgctttcaca agatattttt tgaaaaaaaa ccatgctttt
    69541 attgcaatgt ctgaaaaagt aaaaaacgac ttgctaacat ttttaccaga agcaaaatgt
    69601 gaattattgc cacatccgct ttataatcat tttggagata ttcaagacaa aacaaaagct
    69661 aaagaaacat taggaataga accaaataaa aagactttat tatttttcgg ttttattcgt
    69721 gattataaag gtttggatat tttaatagaa agttttggag aacttgataa gtcatatcag
    69781 ttgcttattg ctggcgaagt ttatggaaat ttttcaaaat acgatgaatt aatcaataaa
    69841 aatccaaaca aagaaaatat ccataagcat ataaaatata tttcagacaa tgaagtaagt
    69901 acatattttt cagccagcga catttgtgtt ttaccatata aatcggctac ccaaagcgga
    69961 ataacaggaa tttcatttca ttttgaaaca ccaatgatag caacagatgt tggaggattg
    70021 gcagaaatta tttc
//
LOCUS       DAOPNX010000003        59100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_677_length_59100_cov_6.528868, whole genome shotgun sequence.
ACCESSION   DAOPNX010000003 DAOPNX010000000
VERSION     DAOPNX010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 59100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 59100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59100
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_677_length_59100_cov_6.528868"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2998)
                     /locus_tag="PKN53_00545"
     CDS             complement(<1..2998)
                     /locus_tag="PKN53_00545"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HOD87475.1"
                     /translation="MFNAFLFGQEKNGVSELSWINKVETDAEKFYENGTRICYLNIYG
                     EIPETYVQSIANQVLANSGVLRFHLYYGKEYSRCMCETKDDFTNEQLAVILDEIIDKY
                     YDSGEYLIDNYDAIIEKVSSNGVYAKRGDSESQPPSDDDYEPTVTNYRAGSACSPTTS
                     GGSTYYITNFTTTGGTTNINNSSGSSASGYQDFYNTVSMSANTGATINYSISCSSSGS
                     TYGRAIWIDWNENGVFEASEQVVSSGSYASSPLTGSFSIPTGVSSGTKRMRILASYTP
                     SNPSNPCSNSGSGEYEDYKIIIQAIEMKVPAAGNNSYTTCSGHLYDNGGASGNYANSS
                     NGYTVLYPGTTGQTVQVSGTITCEGGYDYLTIYNGVGTGGTVLWGGSAHGSGTGCATF
                     TVPAITSTTGPLTVKFNSDASNNCAGFDLTISCVPAQGTGEDCTSSIAFCTEDNYLFP
                     ASTNVTGYGQVGCLYSTPNPAWYYMQVGDPGDIIIYISSGYDVDFIAWGPYSSVDTYC
                     SEITMGPCTPSSCPNNTSNPSFYPYGDIVDCSYSAASYETVHILGAQTGEVYALLITN
                     YSNQVTNISFQQTSGSGTADCSIVAPPISSNSVVCVGNTLELYVTYSQPGATYQWTGP
                     GGWTSNQMNPTRPNATTAMSGTYSMVVTIGGLTSDPVTTQVTVAPNVTPTFNAVGPYC
                     QGASIPALPTTSTNGITGTWSPAINNNATTTYTFTPNAGQCASTTTMQIVVNPLPSPS
                     INPNPATICAGGSIQLTASGGATYNWSNSSTSPSINVSPASTTTYTVTVTSAEGCTAS
                     ASRQVTVNPLPTVAATVDCGLGVGQGVINVTSPTGANYQYSLNGGSFQSSPSFGNLAN
                     GSYTVVATNTTTTCSSPESIVVVDCGCANPPTVTLATTSGEICGISSPFTLGGNTFGG
                     SATAVTLSHNGGGNLNITSATSSPFSFTYTPAASDLGNTVTIIVTTNNPIGLPCTVEQ
                     KTLSLNVLTLPTVVVGYNPPVCETGTL"
     gene            3436..4908
                     /locus_tag="PKN53_00550"
     CDS             3436..4908
                     /locus_tag="PKN53_00550"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOD87476.1"
                     /translation="MRKFLFTYIIFCLFALFVHNNAFSQYANNRKFISSLKTNLNLLN
                     YDESDVLEYCADINFSELSTESEFVNLGNEVYSLNNIAYNLYSDLQNLDHKTKCFLLY
                     AMADLKTKNNFLYKDSEYLKNLDYLPILVSALDKHFVNSTTAYGLWALQFMPALRYGL
                     SIDTCYDQRLEPQLSSQAAGKYLMDLQKSFGSWDYAIMAYVCGPTVVRKAMKNTNSFD
                     ETVNNLEANYKNYFYYYLAIVKWFSEKEIINPSFFVSEKFYDKDTVFVKDRLHFEQIS
                     EYLQLNLKELKEMNPLFVGNIIDGRNSEKEIYLPYGYKDRFIANKDSIIAFKDSIYFP
                     VFQMPNVSDRSNANYVSISPGKDYEEIKHTIQTGDNLGSIAQKYGVRVSDLQDWNNIR
                     GTNIYAGKVLSIWVKKGSALASNANSNKNDNNKATKPKPFNAAGYELVETITIAKGDS
                     FYKISKNYSWASAEDIMYWNGIEDASKLQIGQKLKIYKKK"
     gene            4908..6365
                     /locus_tag="PKN53_00555"
     CDS             4908..6365
                     /locus_tag="PKN53_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87477.1"
                     /translation="MKVRHVLYFVVSVIALLGVIMFIFPEDGIRINKNWVLYFPSFEE
                     MFLDSKKATVSADSLINSQIDINEIIPEENINEIDSIDFEELKKSITPLEFPEDNPHA
                     LDNFYSKLHNMDKEKRVRVMHYGDSQIEGDRISSFIRSKFQTRYGGLGIGLCSPVAIY
                     AQYSLKQANSANWYRYNGFAYSENISPHKKYGPMIAYNRFAPIPDSATWEATTKYSAW
                     LEFSESNLGYSNTKTFKNIAIYYGNSKTKVQIKVISDGHELAVDSLEAGEDLYVYRYN
                     SDTYLNNVRLEFEAYDSPDFYAISLEDDTGVFVDNIALRGSSGTVFTTTDGSLLAKSF
                     AELGVDLFILQFGGNTVPYIEDAKSAERFANHFYYQLAYLKNVVPDVNIIVIGPSDMA
                     TKIKDDYQTYPNLENIVDELRKATHRAGCVYWDMYKAMGGENSMIAWVNANPPLASPD
                     FVHFTPNGANVVSNIFYNSLIINYAEYLERAKNAK"
     gene            6355..7614
                     /locus_tag="PKN53_00560"
     CDS             6355..7614
                     /locus_tag="PKN53_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOD87478.1"
                     /translation="MPNSFKIFLIGFVSIFVFQNNAKAQVSRHLFSEYKYEYIQYDSC
                     FLESFGNNKNFKTFYSKLEKLNFTGEGQISILHLGGSHIQAGIWSWQMRKNFETMMPG
                     MEGTLGLVFPFCIAKTNHPFYYTSKVEGKWEFQRITDKEVDVNIGLSGITAFTSDSTA
                     SILISFNPVSEIEKHKFDKFSIFHDVEDTCWNLTVKPEEFLDTVIVNFETGASEFYFT
                     EKLDSVQLFVTKKDSSDSKFYFYGAYLENSDPSINYCGIGINGASTSSYLKAELFEKH
                     LKLVNPDLVILSIGVNDAASKSFKESVYVENYKKIIDKILNINPNCAILLTTNNDFYY
                     YRGGVNPFSSQVYSSMKTLAIHYGGSVWNLFKAMGGLKSVNLWRKDELANRDRIHFTR
                     AGYIIVADLLFDAIIKDFAKFLQENKT"
     gene            7629..9293
                     /locus_tag="PKN53_00565"
     CDS             7629..9293
                     /locus_tag="PKN53_00565"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOD87479.1"
                     /translation="MSFLEFIKNIFTYSEESPLIFTRFFFWGFFAIVLLGYCFVHKRF
                     ALRSLYLLIVSLFFYYKSSGFFFLLLIFNTLFEYFVGLSLYKSKSEIYRKLLLAFSIV
                     VNLFILSYFKYTYFFTSVINDLFGTNLVAYNYLADFSNLLTGSHFDTSRIFLPVGISF
                     YTFQTMSYTLDIYKRKLEPVKNIIDFGFYVAFFPPLVAGPIVRAADFIPQIYKKFQLS
                     RYEFGLAVFMILKGLIKKITIGDYIAVNFVDRIFSNPEMYSGFESFMAMLGYSLQVYV
                     DFSGYTDIAIGVALLMGFRLRQNFNSPYKATNCGEFWKRWHMSLSSWLKDYLYIPLGG
                     NRNASVGTWVNFALILGIFALLAASFWVSIGLLIFGVFIISLMFIFPKSKKNIITNIN
                     LMITMLLGGLWHGSSWNFMIWGGLNGIGIVVYKFWRKISPWEKYNNVLATVWKIALTF
                     GFVTFTRVFFRAPDYDTAATMLHRIWNNFSPEVIPAVMAGYYKVWLIMLFGFITHWLS
                     NKFKDKWRDKFINAPHWSQVLIAVAIIFMVYQSICSEMQAFIYFQF"
     gene            9297..9962
                     /gene="hxpB"
                     /locus_tag="PKN53_00570"
     CDS             9297..9962
                     /gene="hxpB"
                     /locus_tag="PKN53_00570"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: protein motif:HMM:NF008087.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HOD87480.1"
                     /translation="MLDNFQAVIFDMDGILIDSEPLWRQASMEIFTSLGVEMNEELLN
                     KTSALSSLDFCKVWYSHQPWEGMSFEEIDKEMEKRVLQIIEEKDVTIPDVKQTIDFFY
                     NHGFKIALASNSSLYYVNRVLKKLNVEKYFSIVASAEQVENAKPAPDLYLHVANELKV
                     KPEKCIVFEDSIVGITAAKAANMFVITLPAQEDFSKHEFDIADKKIKSFSQLLKELKK
                     SVV"
     gene            10050..11699
                     /locus_tag="PKN53_00575"
     CDS             10050..11699
                     /locus_tag="PKN53_00575"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOD87481.1"
                     /translation="MRRILFISSFIIALCTGFVGKILAKNKIPAFWTDGKLEIYNYYY
                     GQINDTAYWFRFTDISENEAKGEYFTLTNTTIYADLKEFSFKKNKKNYILNFDNKSLD
                     LKIEANVSNNGFVLKCSKLKSKFLFFKKYVFLGNYSFKKYSKPDFKTFPNRYKEEIFS
                     DITVLKDVEYGKAEGYWTSYNTGSDRYLNVLVKGFLSSLFKETLSLKMDIYMPKTDEL
                     AKRPLIMFIHGGAFYIGDKEVETMVEMCKYFAKRGYVCASINYRLGFKVAKASIERAG
                     YRAIQDGHAALRFLVNKSETYKINPDYIFVGGSSAGGITALNLAFMRNKNRPETSKKS
                     FLYSDLGDIETDGNTLKDKFEIKAVINMWGAVNNLEMIDNSKASVISFHGDKDDVVPI
                     DHGHPFQDIKVGVNSLFLNKTYGSLPIHIKLKENANREFLHIFEGLGHSLNVDENDKI
                     NKNFYTICDESKDFLYEEIVPKSWGIFSIPPTLTNRALSLYEAKASGVKDYFWDIDGG
                     IIISTDKNKVRVVWFKEADKHVLKLSFITELNAGFYSEYEF"
     gene            11708..12322
                     /gene="recR"
                     /locus_tag="PKN53_00580"
     CDS             11708..12322
                     /gene="recR"
                     /locus_tag="PKN53_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018910434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOD87482.1"
                     /translation="MRFNSKSGKYLEEAIMQFSSLPGIGRKTALRFALHLLRRPENEV
                     ENFANAILSLKKDTKFCSVCHNISDSDLCNICADKSRDFSKICIVEGIHDVIALEATS
                     QYNGLYHVLGGVISPMEGISPSDLNIASLFSRLDEGNVQEVIFALPATMEGDTTNYFI
                     NRKIENKNIEVTILARGVAIGDELQYTDEITLGQSIINRKPFEK"
     gene            complement(12850..13218)
                     /locus_tag="PKN53_00585"
     CDS             complement(12850..13218)
                     /locus_tag="PKN53_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87483.1"
                     /translation="MELYFYGKSNPDTVTIEFISLGSCPVSFLTFKGFDKNFKLKYSY
                     SLFNDKLKIPTNQLKYFQIIAFNANSKLFSIDDFFIKENMMVFYDYIDPRNIEKYKFF
                     DMRIKNYCLSIWKPSLWSVF"
     gene            complement(13578..14030)
                     /locus_tag="PKN53_00590"
     CDS             complement(13578..14030)
                     /locus_tag="PKN53_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOD87484.1"
                     /translation="MSILDRINEDIKKAMLAKEKEKLEALRAVKAAFLLAKTEAGASE
                     ELAYEKELQIIQKLIKQRRDAAEIYKQQNRMDLYEPEEFQAEIISQYLPAQLSEEELR
                     LEISKIIQETGASSIRDMGKVMGIANSKLVGKADSKSISGIVKQLLTS"
     gene            complement(14043..15326)
                     /gene="ftsZ"
                     /locus_tag="PKN53_00595"
     CDS             complement(14043..15326)
                     /gene="ftsZ"
                     /locus_tag="PKN53_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOD87485.1"
                     /translation="MTELEFAIPREESSIIKVIGVGGGGNNAVNRMHELDVKGVDFIV
                     CNTDSQALLISPVATKITLGASLTEGRGAGNNPEKGKEAAIESLPEIEKVLDNNTKMV
                     FITAGMGGGTGTGAAPVIAKAAKERDILTVAIVSIPFLIEGATRINQALQGINEMKQY
                     VDALLVINNERLNEIYKNMKFTDAFKLADDVLATATKGIAEIITVTGYINVDFEDVKT
                     VMKDSGVALMGSATAEGEERDVEAIKQALEFPLLKDNDIKGAKNILLNIISSKGDYEL
                     GMSEFGRITNYLKEVVGKDTNTNIIWGTGFDDSLGEKISVTIIATGFDANKELYIKQD
                     KIKVNLLDDDFEEDEFDIDEKEEDINPEIGQNTIELELDFSPSSQTESEIILPNLDPK
                     NYEHMKDLNKLNDTNFLEGLENQPANERMKNIDKD"
     gene            complement(15370..16686)
                     /gene="ftsA"
                     /locus_tag="PKN53_00600"
     CDS             complement(15370..16686)
                     /gene="ftsA"
                     /locus_tag="PKN53_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOD87486.1"
                     /translation="MENNNEIIAALDIGTTKIVAIAGYKTKDNNFEVVSFGTSPSHGI
                     QRGIVVNIDETVRSIKNAILKAEHFSDYKFSEVFVGIAGQHVLTTTTSCALALGDDEE
                     EIQESHLLVLQDQIISLANNPGEEIIDIIPQSYIVDKKPGVANPIGMKASTLQGNFHV
                     ITGKMGMVNNIKKCVQLCDLKIKSLILEPIASSEAVLSKEDKENNVVMIDIGGGTTDL
                     AIFQNNKIIKTAVIPIGGNTITADIVNTFGLSEKQAEKLKIAYGAAIEEKSHKETLLS
                     IEVRKQDVLDISLSELTRIISSRLSEILFSVKFQIENTSVKNDLEAGVVITGGGSLTK
                     NLKQLATFVLGNSVRIGYPNTFIVGEYAENINNPQFSTAIGLIIKGIEAFEIEAEELR
                     KLDLINQKLNEEKEEEEKDTKTKNKDKNLVKKFVNTFLNLLDEDVN"
     gene            complement(16690..17457)
                     /locus_tag="PKN53_00605"
     CDS             complement(16690..17457)
                     /locus_tag="PKN53_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87487.1"
                     /translation="MIKVILKILQWAILFVYLIVALSFTNTKRDEILSSTVSINIFGK
                     HNFLNKELVEKLFKENNIVLDSVNIKSLDFNAIEKFLEENPYVKNAEIYNNLDGNITI
                     DLAQKKPVMRVITDQVNLYIDEDCNPMPISENYTAQLIVVTGNLDQEFFKTQNKSKLN
                     RDGLSLLNLFDFVNFINSHELWGSQVEQINISPKGDIELVPRVGNHVIILGNLDNYEY
                     KLKKLETLYLKGFDIVDWNKYSSINLKYSDQVICKKR"
     gene            complement(17454..18833)
                     /gene="murC"
                     /locus_tag="PKN53_00610"
     CDS             complement(17454..18833)
                     /gene="murC"
                     /locus_tag="PKN53_00610"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOD87488.1"
                     /translation="MNKLYENIYFIGIGGIGMSALAQYFLNQGFRVSGYDRVRNEMCI
                     RLEKLGITIHYEDNINMINSYFKNPENTLVVYTPAIPTTHSELNWFKNNNFKIQKRSE
                     TLGNITSHKKTIAIAGSHGKTSVSGISAHIISNTEKSCTAFIGGILKNRNSNFIINAK
                     SENIVVEADEYDRSFLNLEPSLALITSVEEDHLDIYENLENISSAFQDFANKTKNKGI
                     VILNSKIDPKFKENLRKDLNIYHYGLNNENCDFYLYNIRYENGECYFDIKFLKDELKD
                     IKYIFPGNHNFENVLAAASISYLSGVSLEEIRKGIETFKGIVRRFDYIVKNHEHIYID
                     DYAHHPSEISAFLTAVRQIFPNKKLTVIFQPHLYSRTQSFYEEFAKSLSISDDLILLP
                     IYPAREEPIPGINSELILSLVSTKEKQVCEKNNLIELIESKKPELLVTMGAGDIDQFV
                     QKIKHIFIK"
     gene            complement(18826..19929)
                     /gene="murG"
                     /locus_tag="PKN53_00615"
     CDS             complement(18826..19929)
                     /gene="murG"
                     /locus_tag="PKN53_00615"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006281599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOD87489.1"
                     /translation="MKNLRIIISGGGTGGHIFPAISIANALKAKWADTEILFIGAEGR
                     MEMEKVPAAGYKIIGLPVIGLKRKISLSIFTFIFKTIKSYRKAKKIIKEFKPDVVVGV
                     GGYASGPALKAAASLKIPYLVQEQNSYPGVTNKLLAKKASKICVAYDNMDKFFPAEKI
                     IKTGNPIRADIQNITNKSSEAFKFFDLNPDKATVLVIGGSLGARTINNSIANTLNEYV
                     KSDVQVIWQTGKHYFDKADEIVGEINSDLIFVSDFIYRMDYAFSVADIIISRAGAGSI
                     SELCVVGKPTILVPSPNVAEDHQTKNALALSTVNAAILVKDSDAEKELTKQTLELLNN
                     KEQREILAKNIKKLELADAANIIADEIKKLANE"
     gene            complement(19916..21193)
                     /locus_tag="PKN53_00620"
     CDS             complement(19916..21193)
                     /locus_tag="PKN53_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HOD87490.1"
                     /translation="MGKIKKKIFKGDMGIWIIILLLSIYSMLAVYSASSQLAYREAGG
                     NTTFYLIRQAVMLMIGVATILVVHNIPYKYFSRASVFLWYLAIGLLVFTLFFGINVNE
                     ARRWIMLPGGITFQTSDFAKVALIMYIARNLAIKEEEVNTWKGCFKKLYFPILLTVGL
                     ILPANLSTAILLFGTTMLLIFFSNLKFKYFAAMIGASLVLGILFFGAVKTFKHVGRFE
                     TWNNRIENFTNKTDKEENIQEKHSKIAIAKGGLFGLGPGKSNQRNILPHPYSDYIFAI
                     IIEEYGLIFGAIPLIVLYLYLFMRVRRIVKNTNRKFGAYIVSGLAALIVLQAFSNMAV
                     TTNVIPVTGQPLPMVSYGGTSIIFMGMALGIILSVSREVEEMKDAKKSQGMEDDIPNA
                     EIENADSEVIEPDEKVLEESLNLVINTKENEES"
     gene            complement(21203..22552)
                     /gene="murD"
                     /locus_tag="PKN53_00625"
     CDS             complement(21203..22552)
                     /gene="murD"
                     /locus_tag="PKN53_00625"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOD87491.1"
                     /translation="MEERIVILGAGESGTGAAILAAVKGYDVFVSDKGSIDEKYKTLL
                     KQYNVNFEENKHSEDIILSASEIIKSPGIPDSVPMIKKIKEIGTPIISEIEFAARYTD
                     AIKICVTGSNGKTTTTMLIYHILQKAGLNVGLAGNVGQSFAYQVATGNFEYYVIELSS
                     FQLDTMFDFKSELSVLLNITPDHLDRYDYKFQNYVDSKFRILQNTTFKDVFIYCSDDQ
                     TIMQEISKREILAQQFEFTQNKDEIENGALLNKNTNEINYITNHKQDFTMQINELSIQ
                     GKHNVYNSMAAGIVAQVLNIRKDVIRESLRDFKSIEHRLESVISVHGIKFINDSKATN
                     VNSAWYALESVNSPIIWIVGGIDKGNDYDSLKDLVREKVKAIVCLGKDNTAIHKAFAD
                     IVPKIVDTTSMPEAVNTAYYLGKKNDTVLLSPACASFDLFENYEDRGNQFKLAVRAL"
     gene            complement(22554..23795)
                     /gene="mraY"
                     /locus_tag="PKN53_00630"
     CDS             complement(22554..23795)
                     /gene="mraY"
                     /locus_tag="PKN53_00630"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOD87492.1"
                     /translation="MLYYLFSYLQELDVPGAGVFEYITFRAAMATITSLIVSIFFGKK
                     IIRLLQKRQIGEEIRDLGLEDQMKKKGTPTMGGLIIIAAIIIPTLLFAKLDNIYVLLM
                     LITTVWLGTIGFLDDYLKIKNKNKVGLKGKYKIIGQIALGFIVGGILYFSDDVVIRTK
                     VCGTTNQEVVEVVDNQNCSEFVEYVDVKSTKTTIPFFKNNEFDYAMLVWFLGENAEKV
                     AWLVFIIVTILIVTAVSNGANLTDGLDGLATGSSAIIGATLGILAYVSGNRIAAEYLH
                     ILYIPNTGELVIFMAAFIGATIGFLWYNSFPAQIFMGDTGSLTIGGIIAVFAIIIKKE
                     LLIPILCGVFLAESLSVMIQVSYFKFTKRKYGEGRRVFLMAPLHHHYQKKGFPEPKIV
                     TRFWIISLILAVISIITLKLR"
     gene            complement(23795..25252)
                     /locus_tag="PKN53_00635"
     CDS             complement(23795..25252)
                     /locus_tag="PKN53_00635"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOD87493.1"
                     /translation="MNIKNYISEINAVESIFNNDGNFTKIEFDSRNVKENDMFVAIVG
                     SLQDGHEYINQAIEKGASVIICEKMPETLNPKVSYISVKSSSKALGILASNFYDNPSQ
                     KLRLVGVTGTNGKTTIATSLYNLIQKLGYKAGLISTIQVIIDNKKIEATHTTPDQLKL
                     NKYLSEMVDSGCDYCFMEVSSHAIVQNRIAGLQFVGGVFTNLTHDHLDFHKTFKEYLK
                     AKQQFFTSLSPKAFAITNNDDKNGQVMLQNSKAKKISYGIKTVADYRASVLESHFEGM
                     LLQIDNKEVWTNFIGRFNVSNILAIYSTAIELGFESDEVLRNISALKPVEGRFETLHS
                     NNNITAIVDYAHTPDALENVLTTINEINAGEGKIITVVGAGGNRDKTKRPEMACVAAE
                     LSDKLILTSDNPRCEDPEEILKDMLKGVNIVQQKKTIKITDRKEAIKTAVMLANKGDI
                     ILVAGKGHEKYQEINGVKHHFDDKEVLSEIFNLLQ"
     gene            complement(25249..27354)
                     /locus_tag="PKN53_00640"
     CDS             complement(25249..27354)
                     /locus_tag="PKN53_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOD87494.1"
                     /translation="MSSRRGILIKYNLLYLVLIAIGVFFICKIIYFQFFYDKELIDNA
                     LKTTKAYTSIEPRRGDIYSSDGKLLVTSIAYYEVGMDPNSDAITQKIFDENLDSLSLC
                     LSQLLPDKTKQQYRSELIKARDSGSRYYRFSFNLSYTDFKKMSNFPLLRKGRLKGGFI
                     YQKTYRRENLFGELAKRTLGTQPTDRAKGVGLEDSFNEELEGVEGYTINERIAGNLWM
                     PIEDAQYIEPKDGLDLITTINIELQDIVETALIKQLTKYEASFGTAVVMDVKTGKVLA
                     IANKELNSNGEYVEKTNHAVAYAIDPGSTFKLVSAIIALEDGYVTPDDIIDTGDGSEK
                     ISYKTVKDTKKGGYGAISFQRAIEVSSNVAIAKVVYENYKLQPLKFYKGIERLNLTEP
                     LGVSIKGETKPTVNIPYQGTWSGISLVQMSMGYELQITPLQILAFYNAIANNGKFVKP
                     LFAEALADKGKIVKYFEPEVLNEQICSPKTLSQVQKMLVGVVEEGTARNIKSENYQIA
                     GKTGTAQMNYGRDSLQRGYHASFVGYFPADNPEYSCIVSIYEPKNAYYGGSVSAPVFK
                     EIADKIYSLSPKYYKSSAEEELAYSLPSVKKGCKQETSNVFKWLNYEVDIKDVNQTKW
                     ITGVYKGDDIVKLNPVSFSDNTKVPDVRNMGLKDAVVILEDCGLSTQVVGRGRIYRQV
                     PEAGSDLRKGDKIILYLCE"
     gene            complement(27351..27704)
                     /locus_tag="PKN53_00645"
     CDS             complement(27351..27704)
                     /locus_tag="PKN53_00645"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HOD87495.1"
                     /translation="MKKENTSHIKQIRSTIKGLFNGSILVSDFVRRQIPFVVFLFILG
                     LIYISNRYNAESIFRQTDEAKKRIENLRSEKIEIQSPLMQNSRKERVLQLLNEKGSQL
                     KEPTKPPYKISRSKE"
     gene            complement(27701..28597)
                     /gene="rsmH"
                     /locus_tag="PKN53_00650"
     CDS             complement(27701..28597)
                     /gene="rsmH"
                     /locus_tag="PKN53_00650"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOD87496.1"
                     /translation="MYHEPVLLKESIEGLNINSDGVYVDATFGGGGHSAEILSKLSEK
                     GQLIAFDQDKDAHKNKIKDDRLTLIYGNFRYIQNYLEYLGHETVDGVLADLGISSYQI
                     DEYSRGFSYRLGGKLDMRMNDKQETKASDVLNNYDEAKLLYIFNNYCDIPNARRLVSL
                     ILEYRLYNKIETIEEFIEIISPVTPKNKEYKYLSQVFQAIRISVNDEINALNDFLSSC
                     SKITKPGSRLSIISYHSLEDRLVKNLIKTGNTEGKLETDIFGQTDLSFKAVNRKIILP
                     NDAEIEKNPRAKSAKLRIAERI"
     gene            complement(28604..29065)
                     /locus_tag="PKN53_00655"
     CDS             complement(28604..29065)
                     /locus_tag="PKN53_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOD87497.1"
                     /translation="MFVGEYIVKLDAKGRIAFPSGLKKQIPAEDLEKPFVVKKDIYEP
                     CLILYPEQEWDTHMSIMKRKLNPFNRVHARIIRDYHRDSARLPFDGSFRLLIPKKLLD
                     DVNIDKEMVLAGQEGKIEIWSKEEYDKQRMDQEVFAKNIEEILGNDTNFYE"
     gene            29240..30082
                     /locus_tag="PKN53_00660"
     CDS             29240..30082
                     /locus_tag="PKN53_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOD87498.1"
                     /translation="MEEDQSLRIDVDAVLEAKNPKLARRLPRFVKSYLKRIVHQDTIN
                     KFLEESKGKQGIEFVEATIKFLDLKVEIEGFENIPDSGRYIFAANHPLGGLESLVLMS
                     TVNKKFKDFKFVVNDILMFLKPLAVLFLPINKHGSQSRESFTLINEYYASDYQILYFP
                     AGLVSRKIKGKVQDLAWQKSFVNKAVEFKRDIVPIFIEGRNSNFFYNLSRFRKFLGIK
                     ANIEMLYLADEMMKQRGKTIKIVFGEVFPYTNIDKSKNATEWAAYFRNLVYSLQKSKN
                     NQKK"
     gene            30120..31109
                     /locus_tag="PKN53_00665"
     CDS             30120..31109
                     /locus_tag="PKN53_00665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HOD87499.1"
                     /translation="MEAVIPPVETELLLAELTKDKFLRKSNFGNTELYVFNCKDSPNL
                     TKELGRLREITFRMAGGGTGKSYDIDEYDLGEDPYLQLIVWDPDEKQIIGGYRFILGD
                     TVLDNPEEKLATSHLFEFSENFLENYLPYSIELGRSFVQPGYQYGRNARKGMFALDNL
                     WDGLGALTVEYPEMKYLFGKITMYKHFDISARDHILSFLGLYFPDTEKLLTPKDPIAF
                     KKVENVFAGNDYANDYKILNAKVRETGEKIPPLVNAYMNLSPSMITFGTVENDEFGGV
                     EETGILINIADIYASKSDRHIKTYNRRIKLAQIFPRNIKIFNPKRWRSSKKQK"
     gene            31182..32708
                     /locus_tag="PKN53_00670"
     CDS             31182..32708
                     /locus_tag="PKN53_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOD87500.1"
                     /translation="MRIFCFIAFLIFTFNFVFSQNLVENGSFENAWTCPESFTTFAYD
                     RPYPSWLNPNKGTPDQFHPCSLGDASVPFNFAGFMFPADGAAYAGIILRETFDDSTKT
                     YKGVSREYIQTKLLEPLKKEELYCVKLSYANASKSLFSVDALGVTLTNSKIGVKDAGL
                     IIQRPQIINKPGHIMDNFNHWIEMCGTYRAFGGETYLTIGNFFDNTQTNYKINDVEFT
                     DSAFYYAYYYIDDVRVFKIDHDFECGCIDDLSFGSDWMADNYDPRTGYNTKGLLAQNS
                     DDKNRSADGAEGKDLADGTSGKNGSGENSGTDDIDGNGGTDGKDSTGEADGKDSYSGT
                     SGTDKNNLADNNSNTSANSKDSNDGKNLAGNIDGNNSSNFAMLNLKESEITEQAFEKA
                     RVGSKFNLNRIFFEFNSSELLALSYIELDKLVEILTSKPNLRIEVRGHTDNVGTESYN
                     RTLSMKRAQAVYDYLISSGIDKKRMKYRGFGTKVPVASNETEEGRSLNRRVEIMIVEL
                     "
     gene            complement(32714..34441)
                     /locus_tag="PKN53_00675"
     CDS             complement(32714..34441)
                     /locus_tag="PKN53_00675"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOD87501.1"
                     /translation="MLLFFLPQKFFHKQYYKKILFTTAFVFNIVILIPSIIDAFYYPF
                     VFRRTSYDTLLMLVKMKSEMKNLWLDFIVDFWEAIVLFIVFCVILILVLKKFFKYKTF
                     ENRGVKSYLQSSAIFILVLFCSFILMRGGFERRPISLVTAAKYAPTNHSPIVLNSAFS
                     IIRTWNKIGLERKTFFENENELNKYFQGYKSYEKEKFNKKNIVIIILESFSCEHLSSL
                     NPENPLDKNKNFAPFLDSLIYESYFCSNAFANGKRSIEGIPAILSSVPTLMGTPFILS
                     PYINNNIPSIASLLKEEGYYSVFYHGGHNGTMNFDAYAELAKFDKYSGRDEYPYPEHF
                     DGHWGIRDEEYLQFIAEELSKNEQAFIATIFTLSSHHPYTVPEKYENVLPDGPLEIHK
                     SIAYADLALKKFFETASTKEWYNNTLFVICADHSSEPWLDYYKSPAGLFSIPILFYDP
                     ANNLKGEYKKTCQQIDVMPSILDYIGYPHKFFSFGSSVFDSLNTNNTAVSFFNDNYQI
                     ISDSTVIVFSNDKPTKLWNYKNDYHGNINLIDDENYQKKKNDLNLFYQAFIQCYNNTL
                     IDNNIKINP"
     gene            34806..35789
                     /locus_tag="PKN53_00680"
     CDS             34806..35789
                     /locus_tag="PKN53_00680"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOD87502.1"
                     /translation="MNLYSDIYQLLKFNDCVIIPGFGGFIANYSSAKVDFNSQEFFPP
                     SKSIAFNKSLNSNDGLLLNHISKSRNLSWEKAVEEVENFVESVNNSLKNNDVVEFPGL
                     GCFVLNQNVLVFTPFNYNDLLEDSYGLSVFTYPMLKFERKPVFVQTKDKDKKQRKSLR
                     PLVYTLTSAAVLAGLVTLSFHFGLFENQTKNTETAKVVSVENIIKPSITPETTEIAVL
                     PTEEIQEDIIEEEIVESIIEEDFVKPDLTINTNTQSHSVHVIAGVFSSSENAHNLCEK
                     YKSQGFESIILPHNNMYRVSLKSFADTKTAYAELPNLKEKADNQALWVLGE"
     gene            complement(35821..36678)
                     /locus_tag="PKN53_00685"
                     /pseudo
     CDS             complement(35821..36678)
                     /locus_tag="PKN53_00685"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
     gene            complement(36828..37043)
                     /locus_tag="PKN53_00690"
     CDS             complement(36828..37043)
                     /locus_tag="PKN53_00690"
                     /inference="COORDINATES: protein motif:HMM:NF040319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HOD87503.1"
                     /translation="MFKKASSKNLAFVGEISTLAIQALRKIGKDQVTVEEINHIKNIL
                     KNENPKHLQHDLLLAPVWIRKLLTINE"
     gene            complement(37013..37564)
                     /locus_tag="PKN53_00695"
     CDS             complement(37013..37564)
                     /locus_tag="PKN53_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87504.1"
                     /translation="MLLSVLYELVRNGEFVSEFSSEKIAYFLQRFGAQETFKLNFQPN
                     FYGPYSGKVKHVLYYLNGSYIMGYSAKDKKPFEELNLIPDAETEVNEFLNKPENKEHN
                     DIVQKTKAFLSGFYSPFALELLSTIDFIITEKNVHTQEEIIKELEKWSNRKKTLFFIL
                     THRQEKLKLENNLLCLKKQVLKT"
     gene            complement(37447..38070)
                     /locus_tag="PKN53_00700"
     CDS             complement(37447..38070)
                     /locus_tag="PKN53_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOD87505.1"
                     /translation="MIQYKIGNLLNSEAEALVNTVNTVGVMGKGIALQFKNMFPNNYK
                     LYVNACKSKELKIGKLLVTEEETLLSGKKIIINFPTKTNWRLPSEYQYIEAGLVELVK
                     VIEEKNIKSIAIPPLGSGHGGLDWNRVKQLLEKYLADVDCDIYIYEPSTDVQEIMKKE
                     RVKLLLQEQCCFLFCMNWFAMESSCQNFHLKKLLIFFNVLELKKLLS"
     gene            complement(38369..38584)
                     /locus_tag="PKN53_00705"
     CDS             complement(38369..38584)
                     /locus_tag="PKN53_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87506.1"
                     /translation="MSSDKTLRNYAKNKSIEYHGMLWIFDKFVETTALTPKEAKIKLN
                     QLVNFNFLFRNNQKLVEEIEKRLKIWK"
     gene            complement(38581..38880)
                     /locus_tag="PKN53_00710"
     CDS             complement(38581..38880)
                     /locus_tag="PKN53_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87507.1"
                     /translation="MKLKVAVTDANIFIDLYDLGLIKSFFNLDLEIHTTSAVLFELYS
                     EQQEVLKAYQSVGKLEVHNLQEKDFIEIYNENYPNLYPKQINLFYMLPIKLMLVC"
     gene            complement(38907..39965)
                     /locus_tag="PKN53_00715"
     CDS             complement(38907..39965)
                     /locus_tag="PKN53_00715"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOD87508.1"
                     /translation="MKKIFAERLKSARLMKGFSLQDLANALSNQLSRQALHRYEKGEV
                     MPDNDKINLLSKALGVNPDYFFRRTKVELGEIEYRKLSKIPRKEASIIKEKTKEYLSR
                     YLELEEILGLRVEFENPLKNLAAITSYEQVNIAANQLREYWNLGNGAIFNVVELLEDK
                     NIKVVKLDVNDDFDGLQTFVNGSIPVIAYNVLKINKPDRIRFTLLHELAHLLLTFGDI
                     TLKQKETLCFQFAGAMLLPEKTIKAELGDRRNKLSINELGNIKKQYGISMQAIVMRAK
                     DCGIINEHYTKQFFFFIKQMNWKIDEPFEYLGVEESNRFEQLLFRALVEEQISMSKAA
                     SLSNQTLADFRSKHQMIF"
     gene            40307..41554
                     /locus_tag="PKN53_00720"
     CDS             40307..41554
                     /locus_tag="PKN53_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87509.1"
                     /translation="MKFIKSLVLIIILTTASAIIFSCNFSKNNKPDKNDKPAKSIDTL
                     DIDTLSDKIDYPKDTSLDQIAALLAGDNANAFKNIFTDKMTFWNNYKASIDTPWQKIT
                     ETRLSKMDKWTKDEICTKINDTALVFYPFSGPDFLNAFHLFPNANDYILVAKEKLGNI
                     PDLYSMTEEDLDSYLNAVNFGLRDIYQRSYFITLNMDQDLRKHKADGVLPLLYVFLNR
                     TGHEIYEFGYYKLNNDAKSFTKIEKPSNSLKTAECIWFKIRKKGDDKLKNLLYFYADI
                     SNEGFENNPAFLQYLQNLRTCNTFLKSAAYLNHYGNFSNIRNIILNSASVLEDDTGVP
                     FRYFNNDEWTHYLYGVYDKPIPDFSSTYLFQEDLEKAYNTSDVKALNFSLGYHWRDGN
                     QNWMLFVKNVDSTEIETNILVRE"
     gene            complement(41755..43137)
                     /locus_tag="PKN53_00725"
     CDS             complement(41755..43137)
                     /locus_tag="PKN53_00725"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD87510.1"
                     /translation="MKRKLLEQLVEWKNSKRRLPLLLEGARQVGKTYLLKKLFGEQYF
                     EKVVHVNFEKADLELINLFEGNIQPQRIISYLALKFNTIINQDDTLIIFDEIQEVPRA
                     LTSLKYFAEDAPEYYVVATGSLLGIALHSGTSFPVGKVDRLRLYPMDFEEFCWANNMQ
                     NQTEEAHAAIKLLRNPLIASGFKDVFQQYLAIGGMPAVVSEWIENKNLDTVEIILKNI
                     LADYKDDFSKHTSNAESRKIGNLWQSLPQQFAKENHKFFYKTVEPSARGRDYAFALDW
                     LIDAGLIYKINLTAHGDKLPLSFYANNSDFKIYALDVGVLRQLANLPSSVVVGDDNIW
                     SNFGGALAENFVLQQLRSLGFDEAYYWTSNANTPNQPKGRSELDFIISEKKAIYPIEV
                     KSGEQVKAQSLRVFREKYNPELSIRFSLKELEYNEGLLNIPLYYSFLFKDLLEQKEKL
                     PISDFAFKEK"
     gene            complement(43475..44083)
                     /locus_tag="PKN53_00730"
     CDS             complement(43475..44083)
                     /locus_tag="PKN53_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOD87511.1"
                     /translation="MSNSYKYIDPDYTYTDPATGVLRNLQDVTDPEVLLFVESGAVTK
                     RLQELYENPIKIKGIDSLFEIHKHLFQDIYAWAGKKRTVEISKGGKQFFPTTYFNNAF
                     MHIEQLIVDFKKIPQDNKRHLAEKLAEILDNVNYLHPFREGNGRVQREFLRLLALEKG
                     LTLNLNPPDNNSVYERYMKGTVESDIETLTELIYDLIVEKNE"
     gene            complement(44076..44297)
                     /locus_tag="PKN53_00735"
     CDS             complement(44076..44297)
                     /locus_tag="PKN53_00735"
                     /inference="COORDINATES: protein motif:HMM:NF037437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="HOD87512.1"
                     /translation="MFNKIEIDRNNLTIMGVQFSDLKTLESTANALGSNMFEGFEPSP
                     KGIEIIRDYVMGKISLVEFVELAKQKAYV"
     gene            complement(44419..45633)
                     /locus_tag="PKN53_00740"
     CDS             complement(44419..45633)
                     /locus_tag="PKN53_00740"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD87513.1"
                     /translation="MNKLINRKNYIQQLEKLKDVHIIKIVTGLRRSGKSTLLNIFAQQ
                     IIDSGIPENRVHKYNFEKPIFPEEYTWREIYNEILSKTDKHNMNYIFLDEPQQISEFE
                     RLVDALFVEANIDLYVTGSNAYFLSGEIATLLSGRYITIHVLPFSFAEFLESQEDAEE
                     DKYKLFNSYLYETSLPQGVLLRDKGSDLQNRYIQDVYNTIVEKDIKQRYNIQNMRSFD
                     NLSKFLMGAIGSTVSPSSISRAMKQDFQNIHHNTVQTYIDYLVNSYVFYQVNRFDIKG
                     KQQLATQEKYYLVDIGFRNLKLGKFQYQDLGHILENIVYLELNRRAYQIWLGKIDSYE
                     VDFIVKNSQNKIEYYQVTLSMSDPKTAEREIRPLKAIDDNYPKYIISTDLITAEVEGI
                     EHINIVDWLLQE"
     gene            complement(45739..47721)
                     /locus_tag="PKN53_00745"
     CDS             complement(45739..47721)
                     /locus_tag="PKN53_00745"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOD87514.1"
                     /translation="MDLLFDIQLSKEDNLLKRFEEIHDYIYANDGLSPQQTLEEFVKV
                     LFIKIFDENENINKFTISAEEWILLKSGKTNQSFVERISNLFELTKQAYVDVFDCDDR
                     IKLSSIALGFTVNKLQSISLLNSSQDAKGLAFQKFLSHHEKDGRGQFFTPEPVIDFCV
                     EILQPKPNETIIDPACGSGGFLMTALKYLQRNNKNLNTSEIVSNHLFGLDINKSIARI
                     AKMKLLLESNGKTNIICTNSLEDLDSLKLLISQKDSFDIVLTNPPFGAKITNVSTLSK
                     FDLGYKWSNRENKFYKTKTVYTNQNAEILFIERCLQLLKEGGRMAIVLPNGNFENPSL
                     EYLRNYIKLKAKILAIVNLPQETFIPFGTGVKTSLLFLEKDTQNINKQYPVFFGRVTK
                     LGYQGNKNGTPLYQKDKYGQILKDSAGQPKLEEDFSIIVSEYKKFQKETMIECENAFS
                     INYSELDGRFDYDFYSPENRKLFSKLNNVKTVRLGDICEIVKVKSKKLKDKNLTVEYV
                     ELSDINSHSYEIINSTTYQVYELPSRASYELQEGDIITAIAGNSVGTRKHATALVTKE
                     FEGNICTNGFRVLRNFKIDTYYLLYFLKSEVFLRQMLMYRTGAAIPNVSDNDLCNIII
                     QLPNEKIISEISKKMRRAFELRQESKKQIESITLEL"
     gene            complement(47777..48724)
                     /locus_tag="PKN53_00750"
     CDS             complement(47777..48724)
                     /locus_tag="PKN53_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87515.1"
                     /translation="MNLLKNYIKNKFGIDEQKFLEVLKMSPGAEGYLFGSLGELLFKE
                     YAESLGYEVLRIKEKPDGGYNAKNEEARGDFYIRKKGNKKDEWFVVECKGVKSNAEKR
                     SGLTKLSSCLTLLVKHAIDRDKHVKSIYEKGLKTYNNAKDKWEKQNRGVFPKFIWSKK
                     NPGAGIPDLTGVWKSKAEIKEWLDSFSDEDFSENAYWDLKAPIRLIQTHMPSTRTDSV
                     TKIKSTGPLVTEFNILCVDLFLRTGKHEFVFANSLQLNPQGKSPNHLQQNYTIDILTA
                     KDGYKRHKLLEPWYDDIEKCINKTKPKPRKLDKSQLDER"
     gene            complement(48730..48906)
                     /locus_tag="PKN53_00755"
     CDS             complement(48730..48906)
                     /locus_tag="PKN53_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOD87516.1"
                     /translation="MKRLRLEKGFSQESLAFSADLDRTYITSIEKGERNVSITVVEKL
                     ANALNVSILEFFKD"
     gene            complement(49099..49224)
                     /locus_tag="PKN53_00760"
     CDS             complement(49099..49224)
                     /locus_tag="PKN53_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87517.1"
                     /translation="MDTSSLILGLVFLVIIILPIIFLARASKKKNNQNENLDQYK"
     gene            complement(49331..49693)
                     /locus_tag="PKN53_00765"
     CDS             complement(49331..49693)
                     /locus_tag="PKN53_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87518.1"
                     /translation="MKSKNYIIIILSFLIFALNANIYNYQSIEIKTNQESGKNNVYFS
                     NLEYFNFTFQKQNFKNDNFVNLKQVNVLFSNKITKPCLQIKSQIIPDNYLVLYKIFIY
                     SKNIFKDYHQVFPFHYFY"
     gene            complement(49696..51234)
                     /locus_tag="PKN53_00770"
     CDS             complement(49696..51234)
                     /locus_tag="PKN53_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOD87519.1"
                     /translation="MLVKSFGSAVNGINAETITIEVNISRGINFYLVGLADSAVKESQ
                     QRIISALTTNNFKWPGKKIVINMAPADLRKEGSAYDLPIAVGILAASEQISFSKLEDY
                     IIMGELSLDGNVNPIKGALPIAINARENGFKGLILPVENTQEAAVVDGIDVYGVKHIM
                     DLVGFLNDSKDLEPVRIDIEAEFQKSVKELEIDFGDVKGQENVKRALEVAAAGGHNII
                     LIGPPGSGKTMLAKRMPGILPPLSLDEAIETTKIHSVAGKTEANKSLLLQRPFRSPHH
                     TISDVALVGGGTYPQPGEISLAHNGVLFLDELPEFKRTVLEVMRQPLEDRVVTISRAK
                     FTVEYPASLMLVAAMNPCPCGYYNHPEKDCLCTQNSIQKYLNKISGPLLDRIDIHLEV
                     TPVNFENLAQTKPAEKSSEIQKRVIQARKIQTERFKNKKNTYCNAQMTSKMIKEFCPL
                     DEESLSLLKNAMEKLDLSARAYDRIIKLSRTIADLENSKDIMPSHIAEAIHYRSLDRD
                     SWGK"
     gene            51354..52385
                     /locus_tag="PKN53_00775"
     CDS             51354..52385
                     /locus_tag="PKN53_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOD87520.1"
                     /translation="MKNFVLFIFCLALTFANLFSQEKKEYKIACVTFYNVENLFDTIP
                     GTNDSEFAPDANKKWNSKKYYAKLDTIASVISKIGKDVTKTAPAIVGLSEIENITVLK
                     DLVKVKDIKNFNYQIVHFDGPDRRGVDCALLYRPEYFKVTNTAMHHVYTPEDPDFRTR
                     DQLLVSGIFDGEEMHFIVLHWPSRSGGEKRSEPKRFAAADVTRSIVDSILTINPKAKI
                     VIMGDLNDDPDNKSVRKRLQTQYKKEKAQLPYLYNATEPLFRNGVGSLAYRDKWNLFD
                     QIIITPEFLEKDKSSYRFYQAHVFNKPYLMQKEGRFKGYPWRTYVGDTYMGGYSDHFP
                     TYIYLIKEK"
     gene            complement(52714..54036)
                     /locus_tag="PKN53_00780"
     CDS             complement(52714..54036)
                     /locus_tag="PKN53_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD87521.1"
                     /translation="MKFYDRTKEIAELQRIKNLSFSEYSRMTVVTGRRRIGKTSLIMK
                     SVEDEPSVYLFVGRKTEATLCNEYIQEISNSLNVFVPQEISSFRSLFQYLMEIGKQRS
                     FHLIIDEFQEFFNINESIYSDIQNLWDQYRRKTKVNLIVSGSVYSLMNKIFQNSKEPL
                     FGRADNTIKLSPFDTDTLKTIISDYNPDYQNDDLLALYTFTGGIPKYVELICDGCELK
                     VDSMIEFIVRENSPFIDEGKNLLIEEFGKNYGTYFSILSCISGGINTQPAIMTALGEK
                     SIGGHIKRLIEDYNILKRVRPFGAKEGSQTVRYEIIDNFLQFWFNYFERNRSMIEIKA
                     FSGLQNIIKSDYTTFSGKMLERYFRAKLAESGLYREIASWWETKGEANEIDIVALLFE
                     KNKALAVEVKRNKERFRPNMFAEKVKRLKQIELPKYTIETKCLSLEDM"
     gene            complement(54151..54843)
                     /locus_tag="PKN53_00785"
     CDS             complement(54151..54843)
                     /locus_tag="PKN53_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD87522.1"
                     /translation="MYYFGVNTLEPEFTYLSGDTVFRYKNNKFNILYNFGAQPGDTWD
                     LGIDTNQWLCSKSIIEVDSVKTITINEMPLRCLFVRPHANSSGYFYGWVIEKFGASSG
                     YLFPLENNCDSLLAIELDDINFSCYKDDSFPLINYTDKDCEYYWDLINVPEFTIDKIS
                     ISPNPIIDKLTVNVSENSAIENIRIFNLQGELKGVYDNSNINICDYQSGLYLLEVRLT
                     DGKTIRKKIIKI"
     gene            55812..56390
                     /locus_tag="PKN53_00790"
     CDS             55812..56390
                     /locus_tag="PKN53_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87523.1"
                     /translation="MAREPRKEIKVERAKTTGWWTWEANWYDENGEWMCGTGGHGSSK
                     SDAKRQGKNAECPCCDSSKLAAKIENVKKIFIEPPLVDAEVVNGYLCNFSMYNPEGEK
                     IKFSTSEISDNTAYWIFGLDCFEPNLSERSELVIAMLKIWGVYRRGKDKKAIMEIHNL
                     TEEYFDIIADKSWSNINIKFDEEDNMYWDIIV"
     gene            56353..57000
                     /locus_tag="PKN53_00795"
     CDS             56353..57000
                     /locus_tag="PKN53_00795"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOD87524.1"
                     /translation="MKKITCIGILLFSITTLIGNFAIAQKIPYMTQIDSKEMKYIDNT
                     EVSISDWREYEFDMKNRFGSNSQEFLSTIPDTALFRRYYNYSFIHPNANNMEKQNFSN
                     ADVGFMKNNYDKYPMIGVSYEQCIDYCKWRTERFNWKIKGVKQIEFSLPSPEDYKKAE
                     SYAEITFNPPLSILKDKKSGKPQGITDNVIEYTLGNKDQVLDRPIGFRCIAKIIK"
     gene            complement(57551..57862)
                     /locus_tag="PKN53_00800"
     CDS             complement(57551..57862)
                     /locus_tag="PKN53_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87525.1"
                     /translation="METALDYDKKPKKKTEVKKFIEKIELVCSSLKESKNENYGFYWD
                     YYVPYFIEMQDGKFIKTFAHIAFATSGYPDVDKWLKKHEKDINNFYEWSSNFNWQQNG
                     K"
     gene            complement(57908..58621)
                     /locus_tag="PKN53_00805"
     CDS             complement(57908..58621)
                     /locus_tag="PKN53_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87526.1"
                     /translation="MKKLILLLVFSLLCSTLFGQQEEIEKLIGEGVYFHDKGDYKSAI
                     DKYDQVLQLDEDNLLALAEKAMSLMYLQKYDDAIKNCKRAIEKHQGDDGLKLVYVTCG
                     NAYDALKETDKSLEMYDEGLKLFPDFFLLYFNKAITLSNIERYEEAIICSQKSVSFNP
                     QHASSHNSLGRLLCVSENRIPAILALSRFLILEPQTNRAKDNLELLQYVMKANVEKTG
                     KNTINVNINPNMLSDTTED"
     gene            complement(58687..58905)
                     /locus_tag="PKN53_00810"
     CDS             complement(58687..58905)
                     /locus_tag="PKN53_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD87527.1"
                     /translation="MFPNPFRDVIYIDNFDGGYGQLKLFNLYGKEVLSTKIYQSSEIV
                     VKDLSAGMYLYKIDFEDGNVFTGKIIKE"
BASE COUNT        19689 a         9673 c         8340 g        21398 t
ORIGIN      
        1 ttaaagttcc tgtttcgcaa acaggtgggt tataaccaac tactactgta ggtagtgtta
       61 aaacatttaa acttaatgtt ttttgttcta cagtacatgg aagtccaatg ggattattgg
      121 ttgtaacaat tattgtaaca gtattaccca aatcagaagc tgctggagta taagtaaagc
      181 taaatggact acttgtagca gaagtgatat tcaaattacc acctccatta tgactaagtg
      241 taactgctgt agcagaacct ccaaaagtat ttccaccaag tgtgaaagga gatgatattc
      301 cgcaaatctc gccagatgtt gttgctaaag ttactgtagg tggatttgca caaccacaat
      361 caacaacaac tatactttcg ggagaagaac aagtagtagt tgtattagtt gcaactactg
      421 tgtaagagcc attagctaga tttccaaaac ttggagaaga ttggaagcta ccaccattaa
      481 gactatattg atagtttgct cctgttgggc ttgttacatt aataacacct tgcccaacac
      541 ctaaaccaca atctacagtt gcagcaactg ttggaagtgg atttacagtt acttgacgac
      601 ttgcactagc ggtacaacct tcagcactag taactgttac cgtatatgtt gttgtacttg
      661 ctggacttac atttatgctt ggagaggttg agctattact ccaattataa gttgcaccgc
      721 cgcttgctgt aagctgaata cttcctcctg cacaaatagt tgctgggtta ggatttatac
      781 taggactagg aagtggattt acaactatct gcatagttgt agtgctagca cattgaccag
      841 catttggagt gaaagtataa gtagttgttg cattattatt tattgcaggg ctccaagtac
      901 ctgttattcc atttgtagaa gttgttggca aagctggaat acttgctcct tgacaatatg
      961 gaccaactgc attaaatgtt ggtgtaacat tcggagcaac agttacctgg gtagtaactg
     1021 gatcactcgt taagccacca atagttacaa ccatagagta agtgcctgac atagctgttg
     1081 ttgcatttgg tcttgttgga ttcatttgat ttgaagtcca accacctgga ccagtccatt
     1141 gataggtagc accaggttga gaataagtaa catacaattc taaagtgtta cctacgcaaa
     1201 ccacgctatt actactaatt ggaggagcaa caatactaca atctgccgtt cccgaacctg
     1261 aagtttgctg gaacgatata tttgttactt gatttgaata atttgtaatt aataaagcat
     1321 aaacttcccc tgtttgtgct ccaaggatat gaactgtttc atatgatgca gcactataac
     1381 tgcaatcaac aatatcgcca tatggataaa aagaaggatt tgatgtatta ttaggacatg
     1441 aagaaggagt acaaggaccc atcgttattt ctgaacaata agtatctact gaactataag
     1501 gtccccatgc tataaaatcc acatcataac cactagatat ataaataatt atatcgcctg
     1561 ggtcaccgac ctgcatataa taccaagcag gatttggtgt tgagtataaa caaccaactt
     1621 gtccgtaacc agtaacattt gttgatgctg ggaaaagata attatcctca gtacagaacg
     1681 caatacttga agtacagtct tctccagttc cttgtgcagg gacacaactt atagttaaat
     1741 caaaacctgc acaattatta gaagcatctg aattaaactt tactgttaaa ggacctgttg
     1801 tagaagtaat agctggtaca gtaaatgtag cacaaccagt tccagaacca tgtgcgcttc
     1861 ctccccacaa aacagttcca cctgttccaa caccattata aatagtaaga tagtcgtatc
     1921 ctccttcgca ggttattgtc ccagacactt gcactgtttg accagttgtc cccggataca
     1981 atactgtata tccattagaa gaatttgcat aattacctga ggcacctcca ttgtcgtaca
     2041 aatgtcctga acatgttgta taagaattat ttcctgcagc aggaactttc atttcgatgg
     2101 cttgaattat aattttataa tcttcatatt ctccactacc gctattacta caagggttgc
     2161 taggattgct aggtgtatag cttgctagta ttctcatacg ctttgtccca gatgaaactc
     2221 cagttggtat tgaaaaacta cctgtcaaag gcgaagatgc ataactacca cttgaaacca
     2281 cttgctctga agcttcaaac actccatttt cattccaatc aatccaaata gctctaccat
     2341 aagtagaacc agaactagaa catgatatag agtaattgat tgttgcacca gtattagcag
     2401 acatagaaac tgtgttataa aaatcttgat aaccgcttgc tgagctaccc gaactgttgt
     2461 taatattagt tgttccacct gttgttgtga aatttgttat ataataagtt gaaccgccac
     2521 tagttgttgg actacaagcc gacccagctc tatagttagt tacagtaggt tcataatcat
     2581 catctgaagg aggttgagac tctgagtctc cccttttagc atatactccg ttgctactta
     2641 ctttttcaat aattgcatca taattatcaa tcaaatactc tccagaatca taatatttat
     2701 caataatttc atctaaaatt acggcaagtt gctcatttgt aaaatcatct tttgtttcac
     2761 acatacatcg cgaatactcc tttccgtaat aaagatgaaa ccttagcaca ccagagtttg
     2821 caagcacttg gttagctata gattgcacat aagtttctgg tatttcacca taaatattca
     2881 aataacaaat acgagttcca ttttcgtaaa acttttcggc atctgtttct actttattaa
     2941 tccaacttaa ttctgataca ccattctttt cttgtccaaa taaaaaagca ttgaacaaaa
     3001 aagaaaagaa aataacaatg actttttttc atattataaa ataattagat aaattatact
     3061 ttacttgtaa taaccgcgat aaagtttcgt ttaaaaatta tacatattaa agaacttcac
     3121 ttaacaaacc ccactttcct ttgcttaaat atcataaata ttaaaaaaaa cgatatttaa
     3181 taattattat acgatacgca aaaattttaa aaaggttgtg taaaacataa aaaaacattc
     3241 tcattttaaa ttagttacac tttttttgaa tagtatttgt gattaaggta gtatttaatt
     3301 ttatttatgc gtataataaa cgagtgcttt tgatcgttga gtaaaaccaa aacttctcat
     3361 aaataggaca ttatatattt aaaacttttt attcccaaat tcagaaattt acttattttt
     3421 gtgcgattaa aatctatgcg aaagttttta tttacatata ttatattttg cttgtttgca
     3481 ttatttgttc ataacaatgc tttttcacaa tatgctaata atcgcaaatt tatttctagt
     3541 ttaaaaacaa atttaaactt acttaattat gatgaaagtg atgttttaga atattgtgca
     3601 gacataaatt tttctgaact ttctaccgaa tctgaatttg taaacctagg taatgaagtt
     3661 tactccttaa ataatattgc ttataattta tattctgatt tgcaaaatct cgaccataaa
     3721 accaaatgct ttttacttta tgcaatggca gacttaaaaa ccaaaaataa ttttttatat
     3781 aaagattctg aatatttaaa aaacttagat tatttgccaa tattggtttc tgcacttgat
     3841 aaacattttg taaattctac tactgcttat gggctttggg ctttgcaatt tatgcctgct
     3901 ttgcgttacg gcttaagtat tgatacttgc tatgaccagc gtttagaacc acaactaagt
     3961 tctcaagctg ctgggaaata tttaatggat ttgcaaaaaa gttttggctc ttgggattat
     4021 gctatcatgg cgtatgtatg cggtcctact gttgtgcgta aagcaatgaa aaacacaaat
     4081 tcctttgatg aaacagtaaa taatttagaa gctaactata aaaattattt ttactactat
     4141 ctagctattg ttaagtggtt tagcgaaaaa gaaattataa atccttcgtt ttttgtttca
     4201 gaaaaatttt acgataaaga cactgttttc gtaaaagaca gactgcattt tgagcaaatt
     4261 tcagaatatt tacaacttaa tttaaaagaa ttaaaagaga tgaatccttt gtttgttggc
     4321 aatattattg acggaagaaa ttcagaaaag gaaatttatt tgccttacgg atacaaagac
     4381 agatttattg caaacaaaga ttctataata gctttcaaag attcgattta ttttcctgtt
     4441 tttcaaatgc caaatgtcag cgacagaagc aatgcaaact atgtatctat aagtcctggc
     4501 aaagattatg aagaaattaa acacactata caaactggag acaatttagg atctattgct
     4561 caaaaatatg gagttagagt ttctgatttg caagactgga ataatattag aggtacaaat
     4621 atatatgcag gaaaagtttt aagtatttgg gtaaaaaaag gttctgcact tgcatcaaat
     4681 gcgaattcta ataaaaacga caataacaaa gctacaaaac caaaaccttt caacgctgct
     4741 ggttatgaat tagttgaaac tataactata gccaagggag attcttttta taaaatctct
     4801 aaaaattatt cttgggcaag tgccgaagat attatgtatt ggaatggaat tgaagatgct
     4861 tcaaaattac aaataggtca aaaattaaaa atttataaga aaaaataatg aaagtcaggc
     4921 atgttttata ttttgttgtc tctgtaattg ctttattagg cgtaataatg tttatttttc
     4981 ctgaagatgg aatcaggatt aataaaaatt gggttttata ttttccaagt tttgaagaaa
     5041 tgtttttaga ttctaaaaaa gcaacagtat ctgccgactc attaataaac agtcaaattg
     5101 atataaatga aattattcct gaagaaaaca ttaatgaaat agatagtata gattttgaag
     5161 aattaaaaaa atcaatcact ccattagagt ttcctgaaga taatccacat gctttagaca
     5221 atttttactc caagttgcat aatatggata aggaaaagcg tgtaagagtt atgcattatg
     5281 gagattcgca gattgaaggc gacagaataa gttcttttat acgtagtaaa tttcaaactc
     5341 gctacggtgg acttggcata ggtttgtgtt cgccagtagc aatttatgct caatatagct
     5401 taaagcaagc aaactctgcc aactggtatc gatataatgg ttttgcttat agcgaaaata
     5461 tatcgcctca caaaaaatat ggtcctatga ttgcatataa tcgttttgca ccaataccag
     5521 attctgcaac ttgggaagca acaacaaaat atagtgcttg gttagaattt agcgagtcga
     5581 atttaggata ttccaatact aaaactttta aaaatattgc tatttattat ggaaattcta
     5641 aaactaaagt tcaaataaaa gtaatatcag atggacatga gcttgctgta gattctttag
     5701 aagctggcga agatttatat gtttatagat ataattccga cacatatttg aacaatgtta
     5761 gattagagtt tgaagcttat gatagccctg atttttatgc tatttcttta gaagatgata
     5821 ctggtgtttt tgttgacaat atcgctttaa gaggaagttc tggcaccgtt ttcacgacta
     5881 cagacggcag tttattggct aaatcttttg ctgaattagg tgttgacctt tttattttgc
     5941 aatttggcgg taataccgtt ccttatatcg aagatgcaaa aagtgctgaa agatttgcta
     6001 atcattttta ttaccaatta gcatatttaa aaaatgttgt tcctgatgtt aatattattg
     6061 tcatcggtcc aagtgatatg gcaacaaaaa ttaaagacga ttatcaaact tatccaaatt
     6121 tagaaaacat agttgacgaa ttaagaaaag ccacacatag agcaggttgt gtttattggg
     6181 atatgtataa agcaatgggt ggagaaaatt ctatgattgc ttgggtaaat gccaacccac
     6241 cattagcaag tccagatttt gttcacttta caccaaatgg agcaaatgta gtctcaaata
     6301 ttttttacaa ttcattaata attaattacg cagaatattt agaaagagca aaaaatgcca
     6361 aatagtttta aaatatttct tataggtttc gttagcattt ttgtttttca aaataatgct
     6421 aaagctcagg tgtcgaggca tttgttttca gaatataaat atgaatacat tcaatacgat
     6481 tcttgtttct tagaaagttt tgggaacaat aaaaatttta aaacttttta ctcaaaatta
     6541 gaaaaactta attttactgg cgaaggtcaa atttctattt tacatttagg aggctcgcat
     6601 attcaggcag gaatttggtc atggcaaatg cgaaaaaatt tcgaaacaat gatgcccggg
     6661 atggaaggta cacttggctt ggttttcccg ttttgtattg caaaaacaaa tcacccattt
     6721 tattatactt caaaagttga aggcaagtgg gaatttcaaa gaattaccga caaagaagtt
     6781 gacgtaaata tcggactttc tggcattaca gcttttacaa gcgacagtac agcttcgata
     6841 ttaatttctt ttaatccagt ttcagaaatt gaaaaacata aatttgacaa attcagcatt
     6901 tttcatgatg ttgaagatac ttgctggaat ttgacagtaa aacctgaaga atttttagat
     6961 actgtaattg taaattttga aactggagcc agcgaatttt attttacaga aaaattagac
     7021 tctgttcagc tttttgttac taaaaaagat tcgtctgata gcaaatttta tttttacgga
     7081 gcatatctag aaaattcaga cccaagtatt aattattgcg gaattggaat aaatggtgct
     7141 tcaacctcat catatttaaa agcagaattg ttcgagaaac atttaaaact agtaaatcct
     7201 gatttggtta ttctatcaat tggcgtgaac gatgctgcta gcaaaagttt taaagaaagt
     7261 gtttatgttg aaaattataa gaaaattatt gacaaaatct taaatataaa tcccaactgt
     7321 gctattttgt tgacaacaaa caacgatttt tattattaca gaggaggagt taatcctttt
     7381 tcatctcaag tttattcaag tatgaaaact ttagcaattc attatggcgg aagtgtgtgg
     7441 aacttgttta aagctatggg aggacttaaa tctgtaaatt tgtggcgtaa agatgaatta
     7501 gcaaatagag acagaataca ttttacaaga gctggctata taattgttgc agacttactt
     7561 tttgatgcaa ttattaaaga ttttgcaaaa tttttacaag aaaataaaac ttagagaata
     7621 gattgtgtat gagtttttta gagtttatta aaaacatatt tacttatagt gaagaatctc
     7681 ctttaatttt tacaagattc tttttctggg gattttttgc tatagtactt ttaggttatt
     7741 gttttgtaca taaacgcttt gcactaaggt ctttatatct tttgatagta agtttgtttt
     7801 tctactataa atctagcgga tttttctttc tacttttaat tttcaatact ttatttgaat
     7861 attttgtagg cttatcactt tataaatcca agtctgaaat atatagaaaa ttactgcttg
     7921 cttttagtat tgttgtaaac ttatttattc tttcatattt taaatacaca tattttttca
     7981 cttctgttat aaatgattta tttggaacta acttggtggc ttacaattat ttagcagact
     8041 tttccaacct tttaacaggc tcgcatttcg acacttcaag aatatttttg ccagttggaa
     8101 tttcattcta cactttccaa acaatgtcat ataccttaga tatttataaa cgaaagttag
     8161 aacctgtaaa aaatattata gactttggtt tttatgttgc atttttccct ccattagttg
     8221 ccggtcctat tgttagagct gcagatttta ttcctcaaat ttataaaaaa tttcaactct
     8281 cgcgttatga atttggactt gcagtcttta tgattttaaa aggcttgatt aagaaaatta
     8341 ctattggcga ctatattgct gtaaattttg ttgacagaat attttccaat cctgaaatgt
     8401 attcgggttt tgaaagtttt atggcaatgt taggatattc tttgcaggta tatgttgact
     8461 tttctggata tactgatatt gccataggag tagctttgtt gatgggtttt agactaagac
     8521 aaaactttaa ttcgccttac aaagcaacaa actgcggaga attttggaaa agatggcata
     8581 tgtcgctatc gtcttggttg aaagattatt tatatattcc tttaggcgga aatcgaaatg
     8641 caagtgttgg cacttgggta aacttcgctt tgattttagg aatatttgcc ttacttgctg
     8701 caagtttctg ggtaagtata gggttgctga tttttggagt atttataata tcgttaatgt
     8761 tcatttttcc aaaaagcaaa aaaaatatta ttaccaatat aaacttaatg ataacaatgc
     8821 ttttaggagg cttgtggcac ggctcaagtt ggaattttat gatttggggc ggactaaacg
     8881 gaattggtat tgttgtttat aagttttggc gaaaaatatc tccttgggaa aaatacaata
     8941 atgttttagc tactgtatgg aaaatagctt taacttttgg ttttgttact ttcacacgtg
     9001 ttttctttag agcacccgac tatgatactg ccgcaacaat gctacataga atttggaata
     9061 actttagtcc tgaagtaatt ccagcagtta tggcaggata ctataaagtt tggttaatta
     9121 tgctttttgg atttataaca cattggttaa gcaataaatt taaagataaa tggcgtgata
     9181 aatttataaa tgcacctcat tggtctcaag ttttaattgc tgttgcaata atttttatgg
     9241 tttaccaatc catttgttct gaaatgcaag cctttattta ctttcaattc taaaaaatgt
     9301 tagataattt tcaagcagta attttcgata tggacggaat tttaattgat tccgaacctt
     9361 tgtggcgaca agcaagcatg gaaattttca cttctttggg tgttgaaatg aacgaagaac
     9421 ttttaaacaa aacatcagca ttaagctcct tagatttttg taaagtttgg tatagccatc
     9481 aaccttggga aggaatgagc tttgaggaaa ttgataaaga aatggaaaaa cgcgttctgc
     9541 aaataataga agaaaaagat gtaacaattc ctgatgtaaa acaaacaata gattttttct
     9601 ataatcatgg ttttaaaata gctcttgcct caaattcttc tttgtactat gtcaatagag
     9661 ttttgaaaaa attaaatgtc gaaaaatatt tttctatagt tgcatctgcc gaacaagttg
     9721 agaatgctaa acctgctcct gatttatatt tacatgttgc taatgaatta aaagtaaaac
     9781 ccgaaaaatg tattgtgttc gaagattcta ttgttggcat tacagcagct aaagcagcaa
     9841 atatgtttgt gataactttg cctgcacaag aagatttttc aaaacatgaa tttgatattg
     9901 cagataaaaa aattaaatct ttttcccaac ttctaaaaga acttaaaaaa tctgttgttt
     9961 agtaaatttt tgcagaaatt atttttaatt ttgcacaaac agacctaatg aacaaaaaaa
    10021 ttaaaataaa attttttaaa tcaaggaaaa tgcgtagaat attatttatt tcttctttta
    10081 taattgctct ttgcacgggc tttgttggaa aaatcttagc caaaaataaa atcccagctt
    10141 tttggactga tggcaaatta gaaatttaca attattacta cggacaaatt aatgataccg
    10201 catattggtt tcgttttaca gatataagcg aaaatgaagc taaaggagaa tatttcactc
    10261 ttaccaacac tactatttat gcagatttaa aagaattttc cttcaaaaaa aataaaaaaa
    10321 attatatttt aaattttgac aataaatcac ttgacttaaa aatagaagcc aatgtgtcta
    10381 acaatggttt tgttctcaag tgttctaaat taaaatcaaa atttctattt ttcaagaaat
    10441 atgtatttct aggcaattat tcatttaaaa aatattcaaa acctgatttt aaaacttttc
    10501 ctaatagata taaagaagaa atttttagcg acattaccgt tttaaaagat gtagaatacg
    10561 gaaaagctga aggctattgg acttcttata acactggttc tgacagatat ttgaatgttt
    10621 tagttaaagg atttttatct tctttattca aagaaacttt atctttaaaa atggatattt
    10681 acatgcctaa aactgatgag cttgccaaac gtcctttaat aatgtttatt catggtggtg
    10741 cgttttatat tggcgacaaa gaagtagaaa caatggttga aatgtgcaaa tattttgcaa
    10801 aacgaggata tgtttgtgct tcaataaatt atagattggg ctttaaagtt gcaaaagctt
    10861 ccattgaaag agctggttac agagctatac aagatggaca tgcagcttta agatttttgg
    10921 taaacaaatc cgaaacttac aagataaatc ccgattatat ttttgttggt ggaagtagtg
    10981 caggtggaat tactgcttta aacttagctt ttatgcgaaa taaaaacaga cctgaaactt
    11041 caaaaaaatc atttttatat tcagatttag gcgatataga aactgatgga aacaccctta
    11101 aagataaatt tgagataaaa gctgttatta atatgtgggg tgcggtaaac aatttagaaa
    11161 tgattgataa ttcaaaagct tctgtaattt cttttcatgg cgataaagat gatgtggttc
    11221 ctatcgacca tggacatcca tttcaagata taaaagttgg cgttaatagt ttgttcctaa
    11281 acaagactta tggttccttg cctattcaca taaaacttaa agaaaatgca aatcgtgaat
    11341 ttttacatat tttcgaaggc ttaggacaca gtttaaatgt tgacgaaaat gataaaataa
    11401 ataaaaactt ttacactatt tgtgatgagt ctaaagattt tttgtatgaa gaaattgttc
    11461 caaaaagttg gggtattttt tcaattccac ctactttaac aaatagagca ttgtctttat
    11521 atgaagctaa ggcttcaggt gtaaaagatt atttttggga tattgatggt ggaataatta
    11581 tcagcacaga caagaacaaa gtgagagttg tttggtttaa agaagcagac aaacatgttt
    11641 tgaaattatc tttcataaca gagcttaatg ctggttttta ctcagaatat gaattttaaa
    11701 tttttgtatg cgttttaata gtaaatcagg aaaatattta gaagaagcaa taatgcagtt
    11761 tagctctttg cccggaatag gcaggaaaac agctttgcgt tttgccttac atttattaag
    11821 gcgaccagaa aacgaagttg aaaattttgc aaatgcaatt ttaagtttaa aaaaagacac
    11881 aaaattttgt tctgtatgcc acaatatttc tgattccgac ttatgtaata tttgtgctga
    11941 caaatcaaga gatttttcaa aaatttgcat agtagagggc attcacgatg ttatagctct
    12001 tgaagcaact tctcaatata atggattgta tcatgttttg ggtggtgtaa tttcgccaat
    12061 ggaaggaatt agtccttctg atttgaatat tgccagttta ttttccagat tagacgaagg
    12121 caatgttcaa gaagttatat ttgccttgcc cgcaactatg gaaggcgaca caacaaatta
    12181 ttttataaat agaaaaatag aaaataaaaa tatagaagtt actattctag cgcgtggcgt
    12241 agcaataggc gatgaattgc aatacacaga tgaaatcact ctaggtcaat caattataaa
    12301 tagaaaacct tttgagaaat aatatagttc ttatttttac cgttttctca tctccccaac
    12361 ctgctaaaac gcctgtagga aaagggttaa aacaaagaat aaatttaagc taatgctact
    12421 aatatatata tgagtacgat taaaaaaatg aataacagta tgggtattct cctgagccat
    12481 atagctcttc tgtatatttt tatatattta tttttttcat caccattata ggtttcaata
    12541 aattttctaa atttactgta agaaaaaata aattctaaac tagaagtagg tatcccttct
    12601 gcattcaaaa tattaaccat tttaaacata taaacacttg aaaatattcc tagtaaaatc
    12661 catgttataa aaataaaaat agcgataaac gtgttcatgt tattttatta ttttgtgtcc
    12721 ctatcttagc cccctaattc atcggacaaa attatagttt aaaaatataa ttaattttgt
    12781 tttttcaaat ttataaaaag atttactaac ataaacattt acaatcaaaa tattaaaaaa
    12841 cttgtgtttt taaaaaacac tccataagct aggtttccaa atagacaggc aatagttttt
    12901 tattctcata tcaaagaatt tgtatttctc tatattacga gggtcaatat aatcgtaaaa
    12961 aaccatcata ttttctttaa taaaaaagtc atctattgag aatagtttac tatttgcatt
    13021 aaaagctata atctgaaaat attttaattg gttagtagga atttttaatt tgtcattaaa
    13081 taaagaatat gaatatttta atttaaaatt tttatcaaaa cctttaaatg ttaaaaaact
    13141 tacaggacaa gaacctaaag aaataaattc aatagttaca gtatcgggat ttgattttcc
    13201 gtaaaaatat aattccacat gatttaacga atcctttgaa taataactac taactttaat
    13261 actgtctcct ttttgtttta ttatttactg ataaatcatc tggatttgat ggaataaact
    13321 tagctttata gcatttatct ataacaatct ttccatcatc tatttttatc tctatttgag
    13381 aaaatagtaa aaaattaaaa gttaaaaata gcagagtaaa atatgtcttt tttactatca
    13441 ttatttcttt aaaatatcaa caactataat attgctgttt tgtattttct aaaaagcttt
    13501 atattaactt taaagctatc acaccaaact cactctcggt caaccaatta caaacaaaaa
    13561 aacttttgca aaataattta gctagttaat aattgtttta caatacccga aattgatttg
    13621 ctatcagctt ttcctacaag tttagaattt gctattccca taacttttcc catatctctt
    13681 attgaagaag ctcctgtttc ttgaataatt ttagaaattt ctaaacgtaa ttcttcttcc
    13741 gaaagctgtg ctggcaaata ttgtgatatt atttctgctt gaaattcttc tggttcatat
    13801 aaatccatgc ggttttgttg tttgtaaatt tcggcagcat ctcttctttg ctttataagc
    13861 ttttgaataa tttgtaattc tttttcgtaa gctaactctt cgcttgctcc agcctctgtt
    13921 ttagcaagta aaaaagcagc ctttactgct cgtaatgctt caagtttttc tttttccttg
    13981 gctaacatag ctttttttat gtcttcattt attctatcta aaatgctcat agtatttatt
    14041 ttttaatctt tgtctatatt tttcattctc tcatttgcgg gttgattttc taaaccttcc
    14101 aaaaaatttg tatcgttaag tttattaaga tctttcatat gttcataatt tttggggtct
    14161 aaatttggca atataatttc agattcagtt tgtgaacttg gcgaaaaatc caattctaac
    14221 tcaattgtgt tttgtccaat ttctggattt atatcttctt ctttttcgtc tatatcaaat
    14281 tcgtcttctt caaaatcatc atctaataaa ttaactttta ttttgtcttg tttaatatat
    14341 agctctttgt ttgcatcaaa acctgtagca attattgtta cactaatttt ttcgcccaaa
    14401 ctatcatcaa aaccagttcc ccaaataatg tttgtatttg tatcttttcc aacaacttct
    14461 ttaagataat ttgtaattct accaaactca ctcataccaa gctcataatc acctttagaa
    14521 ctaataatat ttaacaaaat attttttgct cctttaatat cattgtcttt gagtagaggg
    14581 aattctaaag cttgttttat tgcttctaca tctctttctt ctccttcggc agttgcagaa
    14641 cccataagag ctaccccact atctttcatt actgttttca catcttcaaa gtcaacgttt
    14701 atatatcctg ttacagtaat tatttcggca attcctttgg ttgcagtagc taaaacatcg
    14761 tctgccaatt taaaagcatc tgtaaacttc atgtttttgt agatttcgtt tagcctctcg
    14821 ttattgataa ctaacagagc atcaacatat tgcttcatct cgtttattcc ttgcaaggct
    14881 tgatttattc ttgttgcacc ttctattaaa aaaggaattg acactatagc tacagttaaa
    14941 atatctcttt ctttggctgc tttagcaatt actggtgctg cacctgttcc tgtaccaccg
    15001 cccattcctg cggtaataaa aaccattttt gtattgttat ctaatacttt ttctatttcg
    15061 ggcaaacttt ctatagctgc ttcttttcct ttttcgggat tgtttcctgc ccctctgcct
    15121 tcggtaagac ttgctcccaa tgttatttta gtagcaacag gacttattaa aagtgcttga
    15181 gaatcagtat tacaaacaat aaaatcaaca cctttaacgt ctagttcgtg cattcggtta
    15241 actgcattgt tacctcctcc tccaacgcct ataactttaa taattgatga ttcttctcgt
    15301 ggtattgcaa attcaagttc tgtcatattt tatttctcct ttttattagt tagttatttt
    15361 ctttattatt taattcacat cttcgtccaa caagtttaag aacgtattaa caaatttttt
    15421 aactaaattt ttgtccttat tttttgtttt agtatctttt tcttcctctt ctttttcttc
    15481 attaagtttt tgatttataa ggtctagttt tcgcaattct tcagcttcaa tctcaaaggc
    15541 ttctattcct ttaataatca aacctatagc tgttgaaaat tgaggattat ttatgttttc
    15601 agcatattcg ccaacaataa atgtgtttgg gtagcctatt cttacactat taccaagtac
    15661 aaatgtagcc aattgtttta gattttttgt taacgaaccg ccaccagtaa ttacaacgcc
    15721 agcttcaagg tcatttttta cagatgtatt ttctatttgg aattttacag agaataatat
    15781 ttcgctaagt ctagaactaa ttatgcgtgt aagttcgctc aagctaatgt ccaatacatc
    15841 ttgttttcta acttctattg acaatagcgt ttctttatga cttttttctt ctattgctgc
    15901 accataagct attttcaatt tttctgcctg tttttctgaa agtccaaatg tattaacaat
    15961 atctgctgta atagtatttc cgccaattgg gattaccgca gtttttataa ttttattgtt
    16021 ttgaaaaatc gccaaatctg ttgtaccgcc accaatatca atcattacaa cattattttc
    16081 tttatcttct ttacttaaaa ctgcctcgct tgaagcaata ggttctaata ttaaagattt
    16141 tatttttaaa tcacatagct gaacacattt ttttatattg ttaaccattc ccatttttcc
    16201 agtaataacg tgaaaattac cttgaagcgt tgaagctttc ataccaatag gatttgcaac
    16261 tccaggtttt ttatcaacaa tataggattg tggaataata tcaataattt cctcgccagg
    16321 attatttgct aaacttataa tttgatcttg caatacaaga aggtgagatt cttgaatttc
    16381 ttcttcatca tcgccaagag ccaatgcaca actagtagtt gttgttagca catgttgccc
    16441 tgcaatgcct acaaaaactt cagagaactt ataatctgag aaatgctcag cttttagtat
    16501 tgcattttta atagacctca ccgtttcgtc aatatttaca acaattcctc tttgtatccc
    16561 atgcgatggc gatgtcccaa aagaaacaac ttcaaaatta ttgtctttgg ttttgtatcc
    16621 tgctattgca acaatctttg ttgttccaat atctaatgct gctataattt cattattatt
    16681 ttccatttgt tacctttttt tacaaattac ttgatctgaa tattttaagt ttatgcttga
    16741 atatttgttc caatcaacaa tatcaaaacc ttttaaatat aaagtttcta atttttttaa
    16801 tttatattca taattatcta aatttcctaa aataattaca tgattaccaa ctctaggtac
    16861 taattctatg tctccttttg gactaatatt tatttgttct acttgcgacc cccataattc
    16921 atgagaattt ataaaattta caaaatcaaa cagatttaat agacttagcc catcacgatt
    16981 taattttgat ttattttgtg ttttgaaaaa ttcttggtct aaatttcctg taactacaat
    17041 taattgtgct gtataatttt cagatattgg cataggattg cagtcctcgt ctatatacaa
    17101 gttcacttgg tcggtaataa ctctcataac aggtttcttt tgcgctaagt ctattgttat
    17161 attgccatct aaattattat aaatttcagc attcttaaca taagggtttt cttctaaaaa
    17221 cttttcaatt gcattaaaat caagactttt aatatttaca gaatctaaaa cgatattatt
    17281 ttctttaaat aatttttcaa ctaattcttt gttcaaaaaa ttgtgtttgc caaaaatatt
    17341 tatactaaca gtgcttgaga gtatttcatc tcttttagtg tttgtaaaac ttaatgctac
    17401 aattaagtat acaaacaaaa tagcccattg caatattttt agaattactt ttatcatttt
    17461 ataaaaatat gtttaatttt ttgaacaaat tggtctatat ctcctgcacc catagtaact
    17521 aaaagttcag gttttttgct ttctatcagt tctattaaat tatttttttc gcaaacttgt
    17581 ttttctttag tacttaccaa acttaaaatt aactctgagt ttattcctgg tataggttct
    17641 tcgcgtgcag gataaatagg caacaaaatt aagtcgtcac taatagatag gctttttgca
    17701 aattcctcat aaaacgattg agttctgcta tacaaatgtg gttgaaaaat tactgtgagt
    17761 tttttatttg ggaaaatttg tcttactgct gttaaaaatg ctgaaatttc gcttggatga
    17821 tgtgcgtaat cgtcgatata aatatgttcg tgatttttta caatataatc aaatcttcta
    17881 actattccct taaatgtttc aattcccttg cgaatttctt caagcgaaac tcctgacaaa
    17941 tacgaaatac ttgctgctgc taaaacattt tcaaaattgt gatttcccgg aaaaatgtat
    18001 tttatatctt tcaactcatc tttaagaaat tttatatcaa aataacactc tccattttca
    18061 tacctaatat tatataggta gaagtcgcaa ttttcattat ttaaaccata gtgataaata
    18121 ttcaaatctt tgcgtaaatt ttctttaaat tttgggtcta ttttactatt taaaattact
    18181 attcctttat tttttgtttt attcgcaaaa tcttgaaaag cagaacttat gttttctaaa
    18241 ttttcataaa tatctaaatg gtcttcttct acacttgtta ttaatgctaa actaggttct
    18301 aaatttaaaa acgatctgtc gtattcgtcg gcttcgacta caatattttc gctttttgca
    18361 tttataataa aattagaatt tctatttttt aaaattccac ctataaaagc tgtacatgat
    18421 ttttctgtat ttgagataat atgtgcacta attcccgaaa cagaagtttt accatgagaa
    18481 ccagctattg caattgtttt tttatgactt gtaatattcc caagtgtttc ggaacgtttt
    18541 tgaattttaa aattattatt tttaaaccaa ttaagttctg aatgagttgt tggtattgca
    18601 ggtgtgtaaa caactaaagt attttctgga tttttaaaat atgaatttat catattaata
    18661 ttatcttcgt aatgtattgt tatgccaagc ttttctagtc taatgcacat ttcatttctt
    18721 accctgtcgt aaccagaaac tctaaaacct tggttaagaa aatactgtgc caaagccgac
    18781 attcctatac cgccaattcc tataaaataa atattttcgt acaatttatt catttgctaa
    18841 ttttttaatt tcatctgcta taatatttgc tgcatctgct aactctaatt tttttatatt
    18901 tttagctaag atttcgcgtt gttctttatt atttaataat tctaaagttt gttttgtcaa
    18961 ttctttttct gcatctgaat ctttaaccaa tattgctgca tttactgttg atagtgctaa
    19021 agcatttttt gtttggtggt cttcggcaac attaggcgaa ggaacaagta ttgtaggttt
    19081 acctactaca caaagttcgg aaatacttcc agcaccagca cgtgatatta ttatatcggc
    19141 aacagaaaaa gcataatcca ttctataaat aaaatcgctt acaaaaatta aatctgaatt
    19201 tatttctcct acaatttcgt cagctttgtc aaagtagtgt tttccagttt gccaaattac
    19261 ttgaacatct gattttacat attcgttcaa agtgtttgct atactattat ttattgttct
    19321 tgctcctaaa cttccgccta ttactaaaac tgttgctttg tcgggattaa gatcgaaaaa
    19381 tttaaaagcc tcgctagatt tattcgtaat attttgaata tcagcacgta ttgggttgcc
    19441 tgtttttatt attttttctg ctgggaaaaa tttatccata ttatcataag caacacaaat
    19501 tttacttgct ttttttgcca aaagtttgtt tgttacacct ggataagaat tttgctcttg
    19561 aaccaaataa gggattttca aacttgcggc agcttttaat gctggtccgc ttgcatatcc
    19621 gccaacgccg acaaccacat caggtttaaa ttctttaatt attttttttg cttttctata
    19681 gctttttata gttttgaaaa taaatgtgaa aattgataaa gaaatttttc ttttcagacc
    19741 aatcactggt agtcctatta ttttatatcc tgcggcagga actttttcca tttccattct
    19801 accttcggca ccaataaaca atatctcagt atctgcccat tttgctttta atgcattagc
    19861 aatagaaata gcaggaaaaa tatgtcctcc tgttcctccg ccactaatta ttattctaag
    19921 attcttcatt ttcttttgta tttataacta aatttaaact ttcttctaat actttctcat
    19981 ctggctctat cacttcagaa tctgcatttt caatttctgc atttggaata tcatcttcca
    20041 taccttgtga tttttttgca tctttcattt cttcaacttc cctactaaca ctaagaatta
    20101 ttcctagtgc cattcccata aatataattg aagttccgcc ataacttacc ataggcaaag
    20161 gctgaccagt tactggaata acatttgttg ttactgccat atttgaaaac gcctgtaaaa
    20221 ctataagagc tgctaatccg ctcacaatgt aagcaccaaa ctttctatta gtatttttta
    20281 caattcgtct aactctcata aataaataca gataaagcac aataagcgga attgctccaa
    20341 aaatcaaacc gtactcttct ataattattg caaaaatata gtctgaatat ggatgtggta
    20401 aaatatttct ttgatttgat tttcctggtc ccaagccaaa caaaccacct ttagctattg
    20461 ctatttttga atgtttttct tgaatatttt cttccttatc agttttgtta gtaaaatttt
    20521 ctattctatt attccaagtt tcgaaacgtc ctacatgttt aaaagttttt actgctccaa
    20581 aaaacaaaat accaaggacc aaactggctc ctatcattgc tgcgaaatac ttaaatttta
    20641 aattgctaaa aaatatcaat agcatagtag ttccaaataa aagtatagca gtagataaat
    20701 tggcaggtaa aatcaaacct actgttaata atattgggaa atatagcttt ttaaagcaac
    20761 ctttccaggt atttacctct tcttctttta ttgctaaatt tctggctata tacattataa
    20821 gtgcgacttt agcaaaatct gaagtttgaa aggttatacc accagggagc attatccatc
    20881 tacgagcttc gtttacatta attccaaaaa acaatgtaaa tactaataag ccaattgcaa
    20941 gataccacaa aaatacagaa gctcgtgaaa aatatttata agggatatta tgtactacta
    21001 atatagttgc tactcctatc attagcatta ccgcttgccg tataagataa aaagtagtat
    21061 ttcctcctgc ctcgcgatat gccaattggc tcgaagcaga ataaacagca agcatagaat
    21121 aaatggataa aagtagaata attatccaaa tacccatatc gcctttaaat attttctttt
    21181 taatctttcc catttttatt ttctataaag ctcttactgc tagtttaaat tgatttcctc
    21241 tatcttcata gttttcgaac aaatcaaaac ttgcacatgc tggcgacaat aaaactgtat
    21301 cgttcttttt gccaagataa tatgcagtgt ttactgcttc tggcattgaa gtggtatcaa
    21361 caatttttgg aactatgtca gcaaaagcct tgtgaatagc agtattatct tttcctaaac
    21421 atacaatagc ttttactttt tctctcacta aatcttttaa tgagtcgtag tcatttcctt
    21481 tgtctattcc accaactatc catattatag gcgaatttac agattctaaa gcataccaag
    21541 cagagtttac atttgttgct tttgaatcat taataaattt gattccatgc acacttatca
    21601 cagactctag cctatgttct atacttttaa aatctcttaa actttctcga attacatctt
    21661 ttcttatgtt taaaacttga gcaacaatac ctgctgccat tgaattgtaa acattgtgtt
    21721 tcccttgaat tgataactca tttatttgca tagtaaaatc ctgtttatga ttagtaatat
    21781 aatttatttc gtttgtgttt ttgtttaata aagctccatt ctctatttca tctttgtttt
    21841 gtgtaaattc aaattgttga gccaaaattt ctctcttgct aatttcttgc attatagttt
    21901 ggtcgtcaga acaataaata aacacatctt taaatgttgt attttgaagt attctaaatt
    21961 ttgagtcaac ataattttga aatttataat catatctatc taaatggtct ggtgtgatat
    22021 ttagcaaaac cgacaactcc gacttaaaat caaacatagt gtctaattgg aaactgctta
    22081 attcaattac gtaatattca aaatttcctg ttgctacttg ataagcaaaa ctttgtccta
    22141 catttcctgc taagccaaca tttaaacctg ctttttgcaa aatatgataa atcaacattg
    22201 ttgttgttgt tttgccgttg cttccagtaa cacatatttt tattgcatca gtatatcttg
    22261 ctgcaaattc aatctctgaa attattggag ttcctatttc ttttatcttt tttatcatag
    22321 gaacactatc aggaatacct gggcttttta taatttcaga agctgataaa attatgtctt
    22381 cgctatgttt gttttcttca aaatttacat tgtattgttt taataaagtt ttatattttt
    22441 catcaatact tcctttatct gaaacaaaaa catcatagcc ttttactgct gctaaaattg
    22501 ctgcaccagt tccactttcg cctgcaccta atattacaat tctctcttcc atcttatctt
    22561 aattttaggg ttataattga tattacagca agtatcaaag aaattatcca aaatcttgta
    22621 acaatttttg gctctggaaa tcctttcttt tggtaatgat gatgaagcgg tgccattaaa
    22681 aacacccttc gcccctcgcc atactttcgc ttagtaaatt taaaatatga aacttgtatc
    22741 attaccgaca agctttcggc aagaaaaact ccacataaaa ttggaattag cagttctttc
    22801 tttataatta ttgcaaatac agcaattatt ccacctattg tcaaacttcc tgtatcgccc
    22861 ataaaaattt gagctgggaa agaattatac cataagaaac caattgttgc tccaataaaa
    22921 gctgccataa atataactag ctctccagta tttggaatgt acaaaatatg taaatattct
    22981 gctgcaattc tgtttccaga gacataggct aaaattccta aagtagctcc tattattgcc
    23041 gacgagccag tagccaaacc atctaaacca tcggttaagt ttgctccatt agacaccgcc
    23101 gttactatca gaattgtaac aataataaaa accaaccaag ccactttctc tgcattttct
    23161 cccaaaaacc aaactagcat tgcatagtcg aactcattat ttttgaaaaa aggtatagta
    23221 gttttggttg atttaacatc aacatattct acaaactcac tacaattttg attatcaaca
    23281 acttccacta cttcttggtt ggttgttcca catactttag ttcttattac aacatcatca
    23341 gagaaataca aaattccacc aacaataaaa cccaaagcta tttgtccaat tattttgtat
    23401 tttcctttta atccaacttt atttttattt ttaattttta aataatcgtc taaaaagcct
    23461 attgttccaa gccatacggt tgtaataagc atcaacaaaa cataaatatt atcaagtttt
    23521 gcaaataaca atgttggaat aattattgcc gcaattatta taagtccacc cattgttgga
    23581 gttccttttt tcttcatttg gtcttctaag cccaaatctc ttatttcttc tccaatttga
    23641 cgcttttgta aaagtctaat aattttttta ccaaaaaata tactaacaat caaagatgtg
    23701 attgttgcca tagcagctct gaaagtaata tattcaaaaa ctcccgctcc tggaacatct
    23761 aattcttgta aataactaaa taagtagtat agcattattg taacaaatta aaaatttcac
    23821 ttaaaacttc tttatcatca aaatgatgtt taacaccatt aatttcttga tatttttcat
    23881 gtccttttcc agcaacaaga ataatatcgc ctttattagc aagcatcaca gctgttttta
    23941 ttgcttcttt tctatctgta atttttatag tttttttctg ttgaactata ttcacacctt
    24001 taagcatatc ctttagtatt tcttctggat cttcgcaacg tggattatca gatgtaagaa
    24061 tcaatttatc gctaagttct gctgcaacgc aagccatttc ggggcgtttg gttttatctc
    24121 tatttccacc tgctccaaca acagtaatta ttttgccttc accagcattt atttcgttta
    24181 ttgttgtcaa aacattttca agagcgtctg gtgtgtgtgc atagtcaact attgctgtaa
    24241 tattgttgtt agaatgtaat gtttcaaatc taccttctac tggttttaaa gctgaaatat
    24301 ttcttaaaac ctcatcgctt tcaaatccta attctattgc agtggaataa attgctaaaa
    24361 tattagacac attaaatctt cctataaaat ttgtccaaac ttctttattg tcaatttgta
    24421 acaacattcc ctcaaaatga ctttctaaaa cactagctct gtaatcagca acagttttaa
    24481 taccgtagga tattttttta gcctttgagt tttgcaacat tacttgtccg tttttatcat
    24541 cattgttagt tattgcaaaa gcctttggag aaagagaagt aaaaaattgt tgctttgctt
    24601 ttaaatattc tttaaaagtt ttatgaaaat ctaagtggtc gtgagttaaa tttgtgaaaa
    24661 ctccacctac aaactgtaaa ccagcaattc tgttttgaac tatagcatga gagctaacct
    24721 ccataaaaca gtaatcgcaa ccagagtcaa ccatttccga caaatattta tttagtttga
    24781 gttgatctgg tgttgtatgt gtagcttcta tttttttatt atcaataatt acttgtatag
    24841 tagaaatcaa gccagcttta taacctaatt tttgtataag attatataaa cttgtagcaa
    24901 ttgttgtttt tccattagta cctgtaacgc caactaatct taacttttga gatggattat
    24961 cataaaaatt tgaagctaaa attccaagtg cttttgatga tgattttaca cttatataac
    25021 tcacttttgg attaagagtt tctggcattt tttcacaaat aattacactt gcaccctttt
    25081 caatagcttg atttatatat tcatgcccat cttgaaggct accaacaata gcaacaaaca
    25141 tgtcattttc tttcacattt ctagagtcga actctatttt tgtaaagttg ccatcattat
    25201 taaaaatgct ttcaactgca tttatttctg atatgtaatt ttttatattc attcgcatag
    25261 atataaaata attttgtcgc cttttcttaa atcacttcct gcttctggaa cttgcctgta
    25321 aattctgcct ctaccaacaa cttgagtact aagtccacaa tcttcaagaa ttactactgc
    25381 atcttttaat cccatatttc ttacatcagg aactttagta ttatcactaa aactaactgg
    25441 atttaatttc acaatatcat cacctttata aactccagta atccatttag tttgatttac
    25501 atctttaata tccacttcat aattcaacca cttaaaaaca ttgcttgttt cttgcttgca
    25561 tccttttttc acacttggta aagagtaagc tagctcttct tctgctgatg atttataata
    25621 ttttggactt aaagaataaa ttttgtctgc tatttcctta aacacaggag cactaacact
    25681 tccaccgtaa taagcgtttt taggttcata aattgacact atacatgaat actctggatt
    25741 gtcggctgga aaatatccaa caaaagaagc gtgataacct ctttgaagac tatctcttcc
    25801 atagttcatt tgtgcagttc ctgtttttcc tgcaatttga tagttttcgc tttttatatt
    25861 tctagcagtt ccttcttcaa ctacccctac aagcattttt tgaacttgac tcaatgtttt
    25921 tggtgagcaa atttgttcgt taagaacttc tggctcaaaa tattttacta ttttgccttt
    25981 atcggcaagt gcttcggcaa aaagtggttt tacaaactta ccattgttag cgatagcatt
    26041 ataaaaagct aatatttgta aaggtgtaat ttgcaactca tatcccatag acatttgcac
    26101 taaagatatt cccgaccatg ttccttgata aggtatattt actgttggtt ttgtttcacc
    26161 ttttatgctc acacctaaag gctctgtcaa atttagtctt tcaattcctt tataaaactt
    26221 taaaggctgg agcttgtagt tttcatatac aacctttgca attgcaacgt ttgaagaaac
    26281 ctctattgct ctttgaaaac taattgcacc ataaccacct ttttttgtat ctttaactgt
    26341 tttgtatgaa attttttcac ttccatctcc tgtatcaata atatcatcag gcgttacata
    26401 accatcttca agagcaataa ttgcagaaac aagtttaaat gttgaacctg gatctattgc
    26461 ataagcaacc gcatgatttg ttttttctac atattcaccg tttgagttta attctttatt
    26521 agcaattgcc aacaccttgc ctgttttcac atccattaca acagctgttc caaaacttgc
    26581 ctcatattta gtaagttgtt ttatcaatgc tgtttcaact atatcttgta gttctatatt
    26641 tattgtcgta attaaatcaa gaccatcttt tggttctata tattgtgcgt cttcaattgg
    26701 catccataaa tttcctgcaa ttctttcgtt tatagtataa ccctctactc cttctagctc
    26761 ttcattaaaa gaatcttcta aaccaactcc tttggctctg tcagttggtt gagtacctaa
    26821 tgttcttttt gccaattctc caaataaatt ttctcttcta taagtttttt gataaataaa
    26881 tcctcctttt agccttcctt ttcttagaag tggaaaattc gacattttct taaaatcagt
    26941 ataactaaga ttaaaactaa atctataata tctagaacct gagtctctgg cttttattaa
    27001 ttcacttctg tactgttgtt ttgttttatc tggcaatagt tgagataagc acaaacttaa
    27061 agaatctaaa ttttcatcaa atattttttg tgttatagca tctgaatttg gatccatgcc
    27121 aacttcgtaa tatgctatag aagtaaccaa caattttcca tcactagaat aaatatcgcc
    27181 tcgccttggt tcaattgaag tataagcttt tgtagttttt aaagcattat caattaattc
    27241 cttatcataa aaaaattgaa agtaaataat tttgcatata aaaaacactc ctatagctat
    27301 taaaactaga tatagcaagt tgtatttaat caatatgcct cgtcttgaac tcattcttta
    27361 cttctcgaaa ttttatatgg tggtttagtc ggttctttta gttgtgagcc tttttcgttc
    27421 aataattgca acactctttc ttttctgcta ttttgcatta aaggagattg tatttcaatt
    27481 ttttcagatc tcaaattttc tattcttttc tttgcttcat cagtttgtct aaaaatactt
    27541 tcagcattat atctatttga gatgtatatc aagcccaata tgaaaaggaa aacaacaaaa
    27601 ggaatttgcc tgcgaacaaa atcacttacc aaaatagaac cgttaaacag tccttttata
    27661 gtagatctta tttgctttat atgtgatgtg ttttcttttt tcatattctt tctgctattc
    27721 ttaattttgc tgactttgct cttgggtttt tctcaatttc tgcatcatta ggtaaaataa
    27781 tttttctatt aaccgcttta aaacttaaat cggtttgacc aaaaatgtcg gtttctaatt
    27841 ttccttctgt gtttccagtt tttataagat tttttaccaa tctatcttct aaagaatggt
    27901 aagaaattat agacaatcga cttccaggtt ttgtaatttt agaacaagaa cttaaaaaat
    27961 catttaaagc atttatttca tcattaacac ttatcctaat ggcttgaaat acctgagaca
    28021 aatatttata ttctttattt ttaggagtaa ctggacttat aatttcaata aattcttcaa
    28081 ttgtttcaat tttattataa agtctatatt ctaaaattaa gcttaccaat cttcttgcat
    28141 ttggaatgtc gcaatagtta ttaaaaatat aaagcaattt tgcctcatca taattattta
    28201 aaacatcact tgccttagtt tcttgtttgt cgttcattct catatcgagt tttccaccta
    28261 agcgatacga aaagcctctg ctatattcat caatttgata agacgaaatt cccaagtcag
    28321 ccaaaacccc atctactgtt tcatgaccta aatactccaa ataattttgg atatatctaa
    28381 aattgccata aattaaagtt agcctatcgt ctttgatttt atttttatgt gcatctttat
    28441 cttggtcaaa tgcaattagt tgtccttttt ccgaaagttt agaaagtatt tctgcagaat
    28501 gtccaccacc accaaaagta gcatctacat aaactccatc tgaatttatg ttcaatccct
    28561 ctatgctctc ttttagtaat actggttcgt ggtacatatc ttcctattca taaaaattag
    28621 tgtcgttgcc caaaatttct tctatatttt tggcaaaaac ttcttgatcc attctttgtt
    28681 tatcatactc ttctttagac catatctcga ttttcccttc ttgtccagct aataccattt
    28741 ctttgtcgat attaacatca tctaacaatt tcttaggaat caacagtcta aagcttccat
    28801 caaaaggaag acgagcacta tctctgtgat aatcacgaat tatgcgtgca tgcactctgt
    28861 taaaaggatt gagtttacgc ttcataatag acatatgagt atcccattct tgttctggat
    28921 acaaaatcag acaaggctcg tagatgtctt tttttacaac aaaaggtttt tccaagtctt
    28981 cggcaggaat ttgcttcttc aagcccgaag gaaaagctat tctccctttt gcgtctaatt
    29041 tcactatata ttctccgaca aacatgtttt aaaatttatg cgtaaaaata tataaaatta
    29101 ctacacaatg caacatttta ctacatttta atacactttg ttaataactt tttatttgaa
    29161 aaaacttaac tatttgataa ccagatgttt gttgttttgt aaaaaacatt tatatttgca
    29221 gaaattaaca agtaataaaa tggaagaaga tcaatcattg cgaattgatg tcgatgcagt
    29281 tttagaggca aaaaatccga aattagctcg ccgtctgcct agatttgtaa aatcatattt
    29341 aaagcgaata gttcatcaag atactatcaa taaattttta gaagagagca aaggcaaaca
    29401 agggattgaa tttgttgaag caaccataaa atttttagac ttaaaagttg agattgaagg
    29461 ttttgaaaac attcccgatt ctggtcgtta tatttttgca gctaatcatc cattgggagg
    29521 tctcgaaagc ttagtattga tgagtactgt aaacaagaaa tttaaagatt tcaaatttgt
    29581 tgtaaatgat attttaatgt ttttaaaacc tcttgcagta ttatttttac ccattaacaa
    29641 acatggctct caatctcgag aaagtttcac tttaatcaac gagtattatg cttctgacta
    29701 tcagatttta tattttcctg caggtttggt ttctcgaaaa ataaaaggaa aagttcagga
    29761 tttagcttgg caaaaaagct ttgtaaacaa agctgtagag ttcaagcgtg atatagttcc
    29821 gatttttata gaaggtagaa attccaattt tttctataat ttatctcgtt ttcgtaagtt
    29881 tttaggaatt aaagcaaata ttgagatgct ttatttagct gatgaaatga tgaagcaaag
    29941 aggaaagact ataaaaatag tttttggcga agtttttcct tatacaaaca ttgataaatc
    30001 gaaaaatgcc actgaatggg ctgcatattt tcgaaacttg gtatattctt tacaaaaaag
    30061 taaaaataat caaaaaaaat gagtattttt acaaaaaatt tttttttgaa atgacgacaa
    30121 tggaagcagt aattccgcca gtagaaacag aacttttact tgcagaatta acaaaagata
    30181 aatttctacg aaagtccaat tttggcaaca cagagcttta tgtttttaat tgtaaagatt
    30241 cgcctaattt aactaaagaa cttggacggc ttcgagaaat tacttttaga atggcaggtg
    30301 gcggaacagg caaatcttac gatattgatg aatatgattt aggcgaagat ccttatttac
    30361 aattaattgt ttgggatcct gatgaaaaac aaatcatagg tggctatcgc tttattttag
    30421 gtgatacagt tcttgataat cccgaagaaa aattagctac ttctcattta tttgaatttt
    30481 ctgaaaattt tttagaaaat tatttaccct actctattga attaggaaga tcgtttgtgc
    30541 aacccggtta tcaatatgga agaaatgctc gcaaaggcat gtttgcttta gataatttat
    30601 gggacggact aggtgcttta acagttgaat acccagaaat gaagtatctt tttggaaaaa
    30661 taacaatgta taaacacttt gacattagtg cgagagatca tattttaagt tttttaggtt
    30721 tgtatttccc tgatacagaa aaattattga caccaaaaga ccccattgct ttcaaaaaag
    30781 tagaaaatgt ttttgctggc aacgactatg caaacgacta taaaatttta aatgcaaagg
    30841 ttagagaaac tggcgaaaaa attccacctt tggtaaatgc gtatatgaat ttatctcctt
    30901 caatgataac ttttggaact gttgaaaatg acgagtttgg tggcgttgaa gaaacaggaa
    30961 ttttaattaa tatcgcagat atttatgctt caaaatccga tagacatata aaaacttata
    31021 atcgtagaat taagcttgct caaatttttc cacgaaacat aaagattttt aatccaaaaa
    31081 gatggagatc ttcaaaaaag caaaaataaa tctgcaaaat atttcttaaa gaataatatt
    31141 ttttgtaatt ttatagtttt attaggagtt aaattgtttt gatgagaata ttttgtttta
    31201 tagctttttt aattttcact tttaattttg ttttttctca aaatcttgta gaaaatggaa
    31261 gttttgaaaa tgcttggact tgtcccgaat ctttcaccac ttttgcttat gatagaccat
    31321 atccaagttg gctaaatcca aacaaaggca ctccagacca atttcaccca tgtagcttag
    31381 gcgatgcttc tgtacctttt aattttgcag gttttatgtt tcctgccgat ggagcagcat
    31441 atgctggaat aattctaaga gaaacttttg acgattctac aaaaacttat aaaggcgttt
    31501 caagagaata tattcaaaca aaattattag aaccattaaa aaaagaagag ctgtattgtg
    31561 tgaaattatc ttacgcaaat gcttctaaat cattattctc ggttgacgca cttggagtta
    31621 cacttacaaa ctcaaaaatt ggtgttaaag atgctggttt aattattcaa agaccacaaa
    31681 tcataaacaa gcccggacac ataatggaca atttcaacca ttggatagaa atgtgcggca
    31741 cttatcgtgc ttttggtgga gaaacttatt taacaatagg taattttttt gataatacac
    31801 aaacaaacta taaaattaat gatgtagaat ttacagactc tgctttttat tatgcttact
    31861 attatattga tgatgttaga gtttttaaaa tagaccacga ttttgaatgt ggctgcatag
    31921 atgatttatc gtttggctcc gactggatgg cagataacta tgacccaaga actggataca
    31981 acacaaaagg tttgttagcc caaaactccg atgacaaaaa tagaagcgca gacggagcag
    32041 aaggaaaaga cttagctgac ggaacaagtg gaaaaaatgg aagtggagaa aacagcggaa
    32101 ctgatgatat agacggaaat ggaggaactg acgggaaaga tagcactgga gaagctgatg
    32161 gtaaagacag ctatagcggc acaagcggaa cagacaagaa caatttagca gataataatt
    32221 caaacacttc tgcaaattca aaagatagca atgatggtaa aaaccttgca ggaaatattg
    32281 atggaaataa ttcgtcaaac tttgctatgc tcaatttaaa agagtctgaa attacagaac
    32341 aggcttttga aaaagcaaga gttggttcta aatttaatct aaatagaata ttttttgaat
    32401 ttaattcttc tgaattacta gctctctcct atattgaatt agataaatta gttgaaattt
    32461 taacttcaaa accaaatttg cgaattgaag taagaggaca taccgacaat gttggaaccg
    32521 aaagctacaa cagaacactt tctatgaaaa gagcacaagc tgtttatgat tatttaattt
    32581 cttcgggtat tgacaaaaaa cgcatgaaat acagaggatt tggtacaaaa gttcctgttg
    32641 catcaaacga aacagaagaa ggacgaagtt taaatagacg tgtggaaatt atgattgttg
    32701 aattgtagcc gttttaagga ttaattttta tattattgtc aattaaagta ttgttgtaac
    32761 actgaataaa cgcttggtaa aacaagttta aatcattttt tttcttctga taattttcgt
    32821 catcaattaa atttatattc ccgtgataat catttttata attccataat tttgttggtt
    32881 tatcattgct aaatactata actgttgaat cggaaattat ttgataatta tcgttaaaaa
    32941 atgatactgc tgtattattt gtatttagac tatcaaaaac gctagaacca aatgaaaaaa
    33001 acttatgtgg atagccgata taatcaagaa tagacggcat aacgtcaatt tgctgacaag
    33061 tttttttgta ttcgcctttt aaattatttg ctgggtcata aaacaaaatt ggaatagaaa
    33121 acaatcctgc cggactttta taataatcaa gccatggttc tgaagagtgg tcggcacata
    33181 ttacaaacag agtgttatta taccattctt tagtcgatgc tgtttcaaaa aatttcttca
    33241 aagccaaatc tgcataagct atagatttat gtatttcaag cggtccatct ggtaagacat
    33301 tttcatattt ttcgggaact gtataaggat gatgagacga aagtgtgaaa atagtagcaa
    33361 taaatgcttg ctcatttttc gaaagttctt cagcaataaa ctgcaaatat tcttcatctc
    33421 taattcccca atgtccatca aaatgctctg gatatggata ctcgtcccta ccagaatatt
    33481 tatcaaattt tgcaagttcg gcataagcat caaaattcat agtgccatta tgtccaccat
    33541 gataaaaaac gctataatat ccttcttctt taagcaaact tgctatactt ggaatattat
    33601 tatttatata aggcgacaaa ataaaaggag ttcccatcag tgttggcaca gaagacaata
    33661 ttgctggtat gccttctata gaacgttttc catttgcaaa agcatttgag caaaaatatg
    33721 attcgtaaat caaagaatct aaaaaaggtg caaaattttt atttttatca agaggatttt
    33781 caggatttaa actgcttaaa tgctcacaag aaaaactttc taaaatgata attacaatgt
    33841 tctttttatt gaatttttct ttttcataag atttatagcc ttgaaaatat ttattcaatt
    33901 cgttttcatt ttcaaagaaa gtttttcttt caagacctat cttattccaa gttctaataa
    33961 tagaaaaagc actgtttaga actattggcg agtgatttgt cggagcatat tttgccgcag
    34021 taactaaaga aattggtctt cgttcaaaac cacctctcat taaaataaaa gaacaaaata
    34081 aaacaagaat aaaaatagca ctagattgaa gatatgactt cactcctctg ttttcaaagg
    34141 ttttgtactt gaaaaacttt tttaatacaa gtattaatat aacacaaaaa actatgaaca
    34201 agactattgc ctcccaaaaa tcaacaataa aatcaagcca taaatttttc atctcgcttt
    34261 tcatcttcac gagcataaga agtgtgtcat aacttgttct acggaaaaca aaaggataat
    34321 aaaaagcatc aataatactt gggattaata ttacaatatt aaatacaaaa gcagttgtga
    34381 aaagaatttt cttataatat tgtttatgaa aaaatttttg aggtaaaaag aataaaagca
    34441 aaaataaaaa atttgcaaag cacaaagaag acaagtcaaa cctaagacct gtaaaataat
    34501 aataaaaaaa ttgtgaatat gtaatgtcga ataaattata attaaatata aaaatcagta
    34561 tcctagctag actatagata aagagcaaaa ttgccatcaa gacaaatact cgcatcaaca
    34621 aagacagctg agatttaatt attttttttt tcatgtttta taagttaccg cttattaaaa
    34681 cctatttttc acatctatga tttacaaaat tagcaattat tattaacaag ggaaaaactt
    34741 gtttaatatt tttaatcttt gttttgattt ttttattatc tttacattct caaaaatagg
    34801 tgaagatgaa tttgtatagt gatatatatc aactacttaa gtttaatgat tgcgtaataa
    34861 ttcccggttt tggtggtttt attgcgaatt attctagtgc aaaagttgat tttaacagtc
    34921 aggaattttt tccgccatct aaaagtatag cttttaataa aagtttaaac tcaaatgatg
    34981 gtttattgct aaatcacatt tcaaagtcaa gaaatctatc ttgggaaaaa gctgtagaag
    35041 aagttgaaaa ttttgttgaa tcagtaaaca attctttgaa aaacaatgat gttgttgagt
    35101 ttcctggctt gggttgcttt gttttaaatc aaaatgtttt agtttttact cctttcaact
    35161 ataatgattt attagaagat tcatacgggc tttcggtttt tacctatcct atgcttaaat
    35221 ttgaacgcaa gcctgttttt gttcaaacca aagataaaga taaaaaacaa agaaaatcgc
    35281 tacgtccatt agtttacact ctaacttctg cagcagttct tgccggactg gtaactttat
    35341 ctttccattt tggtttattt gaaaatcaaa caaaaaatac tgaaactgca aaagttgttt
    35401 ctgtagaaaa tattataaaa ccaagcatta ctcccgaaac tactgaaatc gctgtacttc
    35461 caactgaaga aattcaggaa gatataattg aagaagaaat tgtagaaagc attattgaag
    35521 aagattttgt aaaaccagat ttaacaataa acactaacac tcaaagccat tcagttcatg
    35581 ttatagctgg tgttttttct tcttctgaaa atgcacataa cttgtgcgaa aaatacaaat
    35641 ctcaaggttt tgaatctatt attttaccac acaataatat gtatagagtc agcctcaaat
    35701 cttttgcaga cacaaaaacc gcttacgccg aacttccaaa ccttaaagaa aaagctgata
    35761 atcaagcact ttgggtgttg ggagagtaat actgtttctg aaaattgact tatactttat
    35821 ttacaactta tttatcctgt cttgcaactc ttttaatcgt tttattagtt cgtcaaatga
    35881 caatagactg ccgtaaatca tattggattt catttcttcg taatcaagtt cccacttttt
    35941 tatgattgaa tcaggtggaa taaacataat gcttttggga ttatgtttgt catagtttat
    36001 acctgaaatg ggtgtaaatc ttctgcgatg ttcaacgatt gtattgtaaa gtctagagtc
    36061 attcaatgct attgttgcat agtctgtttg actcaacttt tcaatatcgt ataagtgtct
    36121 gctaagtcgt tcaactctta ttttgtcttg aggtttttga aattcctcgt gcaacaaaaa
    36181 tattttttct aaaaatgttc gctcaggatt tacaacagga atacttatcg gtttatccgc
    36241 gaaaagttta tctttataaa tttgtgaaac aaatgttcca aaagttcttt gcgtaaatgg
    36301 ttctctcaac gaacgactgc caatttctac caaaatacct ggttttaaat aggtatctat
    36361 ttccgtaagt ttattgtaat aaatttcaat aatcaaaggg tcttggtcac gattttctac
    36421 ttcacgaggt tttacattta cttccctaaa acctaactcg gcaaattttt ttttcagttc
    36481 ttcagcaaag acttctttta taaatataaa cgattatttt cttaattttt ttacatcttt
    36541 tttggataat tcgcctgtaa aacctaaaaa ttctctatct aacgccagat caatatcttc
    36601 tgaaaaacgt tgtatcaaat tccaagcttt acttaatgat gttcctcctt taaacgttaa
    36661 agcttttgca cagtccattg aaaaaatcac agacaaagta tgcgtaaccc accaatcttt
    36721 ttctgtggct gatgaagacg gtaatcccac ttttctactt gtttctgcaa acaatctttt
    36781 ttttagtttc atctggcaat tgaaaccatt cctgcaaatg ttcaaattta ttcattgata
    36841 gtaagtaatt ttcgaatcca aacaggtgct aacaacaaat cgtgttgcaa atgcttagga
    36901 ttttcatttt ttaaaatgtt tttaatatga tttatttctt cgacagttac ttggtcctta
    36961 ccaatttttc gtaacgcctg aattgctaat gtactaattt ctccaacaaa agctaagttt
    37021 ttagaacttg cttttttaaa cacaatagat tgttttccaa ttttaatttt tcttgccgat
    37081 gtgtcagaat aaaaaacaat gtttttttac gattgctcca tttttccaac tctttaataa
    37141 tttcttcttg cgtatgaaca ttcttttctg taatgataaa atcaatagtt gaaagcagtt
    37201 ctaaagcaaa gggtgaataa aatcctgata gaaatgcttt agttttttga acaatatcat
    37261 tatgctcttt gttttcgggt ttgtttaaaa attcatttac ttctgtttcg gcatctggaa
    37321 ttaagtttaa ttcttcaaat ggttttttat cttttgcact atatcccatt atatagctac
    37381 cattaaggta gtacaaaacg tgctttactt ttccagaata aggtccataa aaattgggtt
    37441 gaaagtttaa cttaaaagtt tcttgagctc caaaacgttg aagaaaataa gcaatttttt
    37501 cagatgaaaa ttctgacacg aactctccat tgcgaaccaa ttcatacaaa acagaaagca
    37561 acattgctct tgcaggagta gttttactcg ctcttttttc attatttctt gaacatctgt
    37621 acttggttca taaatataaa tatcacaatc cacatctgcc aaatattttt ctaaaagttg
    37681 tttcactcta ttccaatcca aaccaccatg accagaacct aatggtggaa tagcaataga
    37741 ttttatattc ttttcctcaa ttactttcac aagttcgact aaacctgctt caatatattg
    37801 atactccgaa ggtaaacgcc aattagtttt tgtgggaaaa ttaataataa tttttttacc
    37861 cgacagcaat gtttcttctt cggtaaccaa aagtttccca atttttaatt ctttactctt
    37921 acaagcatta acatataatt tataattgtt tggaaacata tttttaaact gcaaagcaat
    37981 gcctttcccc atcacaccta cagtattgac agtgtttacc aaagcttctg cttcgctgtt
    38041 taataaatta cctattttgt attgtatcat attttaaaaa taataatttg gtctaacatg
    38101 cacattagta acatcttcgc caatttgtgc cgttatcaac ggcattaata catttttatt
    38161 aataccacgt ttcacaaagc aaatatacac ttttcttaga agcaaaacta acaatactta
    38221 aagattttta tagcttgttg tgtgttaaga actttagaat ttactttctt tcaataaaaa
    38281 caattaaaat acacattatc taaactgaca taaatacgtg ctaacgacaa actatattgt
    38341 gctcacattt taaaaattca tctgataatt atttccaaat tttcaatcgt ttttcaattt
    38401 cttcaaccaa tttctgattg tttcggaaaa gaaaattaaa attaacaagc tgattcaatt
    38461 ttatcttagc ttctttaggt gttaaagctg ttgtttcaac aaacttgtca aaaatccaaa
    38521 gcataccgtg atattcaata cttttgtttt tagcataatt tctaagagtt ttatcactac
    38581 tcaacacaca agcattaatt ttattggcaa catgtaaaac agatttatct gcttcggata
    38641 aagatttgga taattttcat tataaatttc aataaagtct ttttcttgca gattatgaac
    38701 ttctagttta ccaacagact gataggcttt aagaacttct tgttgttcag aatacaactc
    38761 aaacaaaacc gcagaagtag tatgtatttc taaatcaaga ttaaaaaaag attttatcaa
    38821 tcctaaatca tacaaatcaa taaaaatatt agcatcggta actgcaactt ttaatttcat
    38881 cttttactaa ttgaaatgac acaaaactaa aaaatcatct gatgtttact tctaaaatct
    38941 gccaatgttt gattactcaa agaagctgcc tttgacattg agatttgttc ttcaactaaa
    39001 gcacgaaaca aaagttgttc aaaacgatta ctttcctcta caccgagata ctcaaaaggt
    39061 tcatcaattt tccaattcat ttgcttaatg aaaaaaaaga attgcttagt ataatgttcg
    39121 tttataatac cacagtcttt agccctcata acaattgctt gcatagagat gccatattgt
    39181 tttttgatat tacccaactc atttattgaa agtttatttc tgcgatcgcc caactctgct
    39241 tttatagttt tttctggcaa taacattgca cccgcaaatt gaaaacataa agtttccttt
    39301 tgttttaaag taatatcgcc aaaagttaaa agcaagtgtg ctaattcgtg aagtagtgta
    39361 aacctaattc tatcgggctt attgattttt agcacattat atgcaattac aggaatagaa
    39421 ccatttacaa aagtttgtaa accatcaaag tcatcattaa catctaactt taccacctta
    39481 atatttttat cttctaacag ttcaacaaca ttaaaaattg ctccattacc taaattccaa
    39541 tattctctta attgatttgc agctatattt acttgttcgt aagaagtaat agcggcaagg
    39601 tttttaagcg gattttcaaa ttcaaccctc aatcctaaaa tttcttctag ttccaaataa
    39661 cgagaaagat actctttagt tttttcctta ataattgaag cctcttttcg tggtattttg
    39721 ctcaatttac gatactctat ctcgcctaac tccactttag ttcttctaaa aaagtaatct
    39781 ggatttacac ctaatgcctt acttaataaa ttaattttat cattatcggg catcacttca
    39841 cctttctcgt aacgatgcaa agcctgacga gagagctgat tactcaaagc attagccaaa
    39901 tcctgtaatg aaaaaccttt cattaaacga gctgacttta atcgctctgc aaatatcttt
    39961 ttcattttgt attaatttga attatactgc aaagataata attttagttt acaaataaat
    40021 aatatttata acattaatat ttacaatttg taactacgaa aatacaacat gcgaacataa
    40081 cattagtatt atcatacttt ttatattgat aacaaaatct ataaacccat aaacaaagtt
    40141 gacaaattta gaaaatacat cttactttta aacctataaa aaacattttc cactccctaa
    40201 atatacaaaa ttctttaaca taaaactttt tactggctaa aatattagtt ttatagtatc
    40261 catattgaga aaaaatgtat ttttgcagcg tttttaatat tattttatga aatttattaa
    40321 atctcttgtc ttaattatta tacttacaac tgcaagtgca ataatttttt cttgtaattt
    40381 tagtaaaaac aacaagcctg acaaaaacga caaacctgct aaaagcattg acactttaga
    40441 tattgataca ttatccgata aaattgatta tcctaaggat acaagcttag accaaatagc
    40501 agcattattg gctggcgaca atgccaatgc ttttaaaaat attttcacag ataaaatgac
    40561 tttctggaat aattataaag cttccattga cactccttgg caaaaaatta ctgagacaag
    40621 actttcgaaa atggacaagt ggacaaaaga tgaaatttgc acaaaaataa atgacaccgc
    40681 actagtattc tatccatttt ctggacctga tttcctaaac gcctttcatt tattcccaaa
    40741 tgcaaatgac tatattttgg ttgctaaaga aaaattaggc aatatccctg atttgtactc
    40801 tatgaccgaa gaagaccttg actcatattt gaatgctgta aatttcgggt taagagatat
    40861 ttaccaacgt agttatttca ttactttgaa catggatcaa gacttgcgta aacataaagc
    40921 tgatggtgtt ttacccctac tttatgtgtt tttaaaccgc actggacacg aaatttatga
    40981 atttggttac tataaactaa acaatgatgc aaaaagtttc actaaaatcg aaaagccaag
    41041 caattctctg aaaacagcag aatgtatttg gtttaaaatc agaaaaaaag gtgatgacaa
    41101 actaaaaaat ctactttatt tttatgctga tatttctaat gaaggttttg aaaacaatcc
    41161 tgcatttttg cagtatttac aaaatttaag aacttgtaat acatttctaa aatctgctgc
    41221 atatttaaac cactatggaa acttttcgaa cataagaaat ataatattaa attcagcatc
    41281 tgttttggaa gacgatactg gtgttccttt tagatatttt aataatgatg agtggacgca
    41341 ttacctttat ggagtttacg ataaaccaat tccagatttt tcttctacat atttattcca
    41401 agaagacctc gaaaaagctt ataatacttc tgatgttaaa gctcttaatt tttcgttggg
    41461 ctatcactgg cgtgatggaa atcaaaactg gatgctgttt gtgaaaaatg ttgatagcac
    41521 tgaaatagaa acgaatattc ttgttaggga ataatgttta ataaaaaata cgattgaatt
    41581 tttgtacaaa aaatcacatt attagtacca ataatgtgta aaattccaca tttttagcac
    41641 caatatttag ttaaaatcaa ttttcttaaa agaatgatat taggaaattt gtgcagacag
    41701 cgtctgcaaa attaacattt tgttacttac ataaaatatt aagtaaggat aatatcattt
    41761 ctctttaaat gcgaaatctg agattggtaa tttttctttt tgctctagca agtccttaaa
    41821 taaaaacgaa taataaagag gaatatttaa aagtccttcg ttgtattcca attcttttag
    41881 agaaaaacga atacttagtt cgggattata tttttcacga aacactctca aactttgtgc
    41941 ttttacttgt tctcctgatt ttacttcaat tggataaatt gccttttttt ctgaaataat
    42001 aaaatcaagt tcacttctcc ctttaggctg atttggagtg ttagcattgc ttgtccagta
    42061 atatgcctca tcaaaaccaa gagaacgcaa ttgttgtaga acaaagtttt cggctaaagc
    42121 accgccaaaa tttgaccaaa tattatcatc tccgacaaca actgagcttg gcaaatttgc
    42181 taactgtcgc aacacaccga catcaagagc ataaatttta aaatctgaat tatttgcata
    42241 aaatgacaga ggcaatttgt cgccatgagc agtaagattt attttataaa ttaatcctgc
    42301 atcaatcaac caatcaagtg caaaagcata atcgcgtcca cgagctgatg gttcaacagt
    42361 tttataaaaa aacttatgat tttctttagc aaattgctgt ggcaggcttt gccataaatt
    42421 tcctattttt cttgattccg cattacttgt atgcttgcta aaatcatctt tatagtcggc
    42481 aagtatgttt tttagaatta tttctacagt atcaagattt ttgttttcaa tccattctga
    42541 tactacagca ggcattcctc ctattgctaa atattgctga aaaacatctt taaatccgct
    42601 tgctattagt gggtttctta ataatttgat agcagcatga gcttcttctg tttgattttg
    42661 catattgttt gcccaacaaa attcttcaaa atccattgga tacaaacgta atctatcaac
    42721 ttttccaaca ggaaatgatg ttccactatg caaagcaatg ccaagtaaac ttcctgtagc
    42781 tacgacataa tattcaggag catcttctgc aaaatatttt aaagaagtca atgcacgagg
    42841 cacttcctga atttcatcga aaattatcaa tgtgtcatct tgatttataa tagtgttaaa
    42901 tttcaaagct aaatagctaa taatcctttg aggttgaata ttgccttcaa acaaattaat
    42961 taactctaaa tctgcctttt caaaattaac atgaactact ttttcaaagt attgttcacc
    43021 aaaaagtttt tttagaagat aagttttacc aacttgtcga gccccttcta atagtaatgg
    43081 caaacgtctt ttactatttt tccactcaac taattgttct aataattttc gtttcataaa
    43141 tataaattct atttcgttta caaagatatg aaaaaaaaca cattattagt actaaaaatg
    43201 tgtaaaattt cacattttta gcaccaatgt tttatgattt ggattatttg aaaaatttaa
    43261 acaacgctat ttgcctacta ctcaacaaat tacactctgc tttgtcaaaa cttttaattt
    43321 ttgaaagttt tgataaagtg gttaattgca aaaaaataat ttctttgaaa tgtatgtctt
    43381 atattttagc atttatatgt gtttactttg taaaagtttg tttgtctgta aattattcct
    43441 gaatgaattt attaaagttg aacaaaatat ttttttattc atttttttca acaatcaaat
    43501 cataaattaa ttctgttaaa gtttctatgt cactttcaac agtccccttc atatatcttt
    43561 cgtaaacgct attattatct ggtggattga gatttaaagt caaacctttt tctaatgcca
    43621 ataaccttag aaactctctt tgtactctcc catttccttc tctaaatgga tgcaaatagt
    43681 taacattgtc taaaatttct gctaacttct cagccaaatg tcttttattg tcttgaggaa
    43741 tttttttaaa atcaactatt aactgttcaa tatgcataaa agcattatta aaatatgtag
    43801 tcgggaaaaa ctgttttcct cctttactta tttcaacagt tcgttttttc cccgcccaag
    43861 catatatgtc ttgaaataag tgtttatgaa tttcaaaaag gctgtcaatc cccttgattt
    43921 ttatcggatt ttcataaagc tcttgaagtc gttttgttac agcaccactt tcaacaaaga
    43981 gcaatacctc aggatctgta acatcttgta aattccgcaa aactcctgtc gcagggtctg
    44041 tataggtata gtctgggtct atatatttgt aagaattaga cataggcttt ttgttttgcc
    44101 aattcaacaa attcaactaa tgatattttt cccattacat agtctctaat aatttcaatt
    44161 ccttttgggc ttggttcaaa accttcaaac atattacttc ctaaagcatt tgcagtactt
    44221 tcaagagttt ttaagtcaga gaattgcaca cccattattg ttagattatt tctgtctatt
    44281 tcaattttgt tgaacatata tttagtgttt aaacttcaat tacaaattta taaaattctt
    44341 gaacaaaaag ctaataaaaa cgattatttt aacctagtaa cagattttaa tcaaagttag
    44401 atttgaattt ggtaaaaatt actcctgcaa tagccaatca acaatattta tatgttcaat
    44461 tccttcaact tcggcagtta tcaagtcggt agaaataatg tattttggat aattatcatc
    44521 aattgctttt aatggacgta tttctcgctc tgctgttttt gggtcggaca ttgacaatgt
    44581 tacttgataa tattcgattt tattttgtga gttttttaca ataaagtcaa cctcgtaaga
    44641 gtcaatttta ccaagccaaa tctgataagc tcgtctgttt aattctaaat acacaatatt
    44701 ttctaaaata tgaccaaggt cttgatattg aaacttacct aattttaaat ttctaaagcc
    44761 aatatcaacc aaataatatt tttcttgtgt agcaagttgc tgttttcctt ttatatcaaa
    44821 acgatttact tgataaaata cataagaatt tactaaataa tcgatataag tttgcacagt
    44881 attatggtga atattttgaa aatcttgttt catcgctctt gatatactgc ttggagatac
    44941 agtacttcca attgctccca ttagaaattt tgaaagattg tcaaaacttc tcatgttttg
    45001 aatattgtat cgttgtttaa tatctttttc tacaattgta ttataaacat cttgaatata
    45061 acgattttgt aagtcagaac ctttatctcg caataaaaca ccttgtggca aagaagtttc
    45121 gtaaagataa gagttaaata atttgtattt atcttcttct gcatcttcct gactttcaag
    45181 aaattcggca aaagaaaatg gtaaaacatg aatagttata taacgacccg aaagcaaagt
    45241 agcaatttct cccgacaaaa aataagcatt tgaacctgtt acatataaat caatatttgc
    45301 ttcaacaaac aaagcgtcaa caagtcgttc aaactcagat atttgctgag gttcatcaag
    45361 aaaaatataa ttcatattgt gcttgtctgt ttttgataga atttcattgt aaatttcacg
    45421 ccaagtgtat tcttcgggga aaattggctt ttcaaaattg tacttatgaa ctctattttc
    45481 aggaattcca gaatcaataa tttgctgggc aaaaatattc aataaagtag attttcctga
    45541 acgtcttaat ccagtaacaa tcttgataat atgtacatct ttcaactttt ctaattgttg
    45601 aatgtagttt ttccggttaa tcagcttatt catatattgc tgtttttcat ttagttacgc
    45661 cttactttgt caaaactttc aaaaatcaaa agttttgaca aagatactct tttttttgaa
    45721 aaactgcaat aggattaatt ataattcaag agtaatgctt tcgatttgtt tttttgattc
    45781 ttgtctaagt tcaaaagctc ttctcatttt cttactaatt tcactgatta ttttttcatt
    45841 tggtagttga ataattatat tgcacaagtc attatcagaa acatttggaa ttgctgcacc
    45901 tgttctatac attaacattt gtctcaaaaa cacttccgat ttgagaaagt ataataaata
    45961 ataagtatct attttaaagt ttcttaaaac tctaaatcca ttagtacaaa tattcccttc
    46021 aaattctttt gttactaatg ccgtagcatg ttttcttgtt ccaaccgaat tacctgcaat
    46081 tgcagtaata atatcccctt cctgcaattc ataacttgct ctactcggta attcataaac
    46141 ttgataggta gtcgaattaa taatttcgta tgaatgagaa tttatatctg aaagttcgac
    46201 atattcaact gttaggtttt tatctttaag ttttttactt tttactttta ctatttcaca
    46261 tatatctcct agcctaacag tttttacatt atttaatttt gaaaacaact ttcgattttc
    46321 aggcgaataa aaatcgtagt caaatcttcc atctaattca ctataattta ttgaaaatgc
    46381 gttttcacat tcaatcatag tttctttttg aaattttttg tattctgaca caattatact
    46441 aaaatcttct tctaattttg gttgacctgc tgaatccttc aatatttgtc catacttgtc
    46501 tttttgatat aaaggtgttc catttttatt accttgataa cctaatttag ttactctacc
    46561 aaaaaaaaca ggatattgtt tattgatatt ttgtgtatct ttttctaaaa ataataatga
    46621 tgtttttact cctgtaccaa aggggataaa agtttcttgt gggagattga caatagctaa
    46681 aatttttgct tttaatttta tataatttct taaatattct aaagaaggat tttcaaaatt
    46741 cccatttggc agtacaatag ccattctacc tccttctttt aagagttgca aacaacgttc
    46801 tataaacaga atttcagcat tttgatttgt gtaaacagtt tttgttttat aaaatttatt
    46861 ttctctgttt gaccatttat atcctaaatc aaattttgag agtgttgaaa cattagtaat
    46921 ttttgcacca aatggtggat tagtaagcac tatatcaaaa ctatcttttt gggaaattaa
    46981 aagtttgaga ctgtctaaat cctctaatga gtttgtacaa ataatatttg ttttgccatt
    47041 agattctaat aataatttca tttttgcaat tctcgcaata cttttgttta tgtctaatcc
    47101 aaaaagatga tttgaaacaa tttcagaagt attaagattt ttattatttc tttgaagata
    47161 ttttaaagct gtcattaaaa agccaccgct accgcaagct ggatcaatta ttgtttcatt
    47221 tggttttggt tgcaatatct caacacaaaa atcaatcaca ggttcaggtg taaaaaactg
    47281 acctcttcca tctttttcat ggtgtgaaag aaatttttga aaagccaatc ccttagcatc
    47341 ttgcgatgag tttaataaag aaatactttg aagtttattt acagtaaaac ccaatgcaat
    47401 tgaacttagt ttaattctat cgtcacaatc aaagacatct acatatgctt gcttggtaag
    47461 ctcaaacaga ttagaaattc tttcgacaaa tgattgattt gttttaccag atttaagcaa
    47521 aatccactct tctgcagaaa ttgtaaattt gtttatattc tcgttttcat cgaaaatttt
    47581 gatgaacaat actttcacaa attcttctaa tgtttgctga ggtgataagc catcgtttgc
    47641 atagatatag tcgtggattt cttcaaatct ttttagtaaa ttatcttctt ttgataactg
    47701 aatgtcaaat aataaatcca tattttgatt ttacgcattt gtgacaaaaa taatgcacat
    47761 ttgtgtaatc accaaattat ctttcatcaa gttgactttt atcaagtttc cttggttttg
    47821 gtttagtttt gtttatacat ttctcaatat catcatacca tggttcaagt aatttatgtc
    47881 ttttatatcc atcttttgca gtcaaaatat caattgtata attttgttgt aaatggttag
    47941 gtgattttcc ttgtggattt aattgcaaac tattcgcaaa aacaaattca tgcttacctg
    48001 ttctaaggaa taaatctaca cataaaatat taaactctgt aactaaggga ccagtacttt
    48061 taattttcgt aaccgagtca gttcttgtag aaggcatgtg tgtctgaatc aaccttattg
    48121 gtgcttttaa atcccaatag gcattttcag aaaagtcttc atctgaaaag ctatctaacc
    48181 attcttttat ttcagcttta cttttccaaa cacctgttaa atcaggaatt ccagctccag
    48241 gatttttttt actccaaata aattttggaa aaactcctct attttgcttt tcccacttat
    48301 cttttgcgtt attataagtt ttcaatcctt tttcataaat acttttaaca tgtttatctc
    48361 tatcgatagc gtgttttaca agcaaggtaa ggcaagatga taatttagtt aaaccactcc
    48421 ttttttcggc atttgatttt actcctttac attcaaccac aaaccattca tcttttttat
    48481 ttcctttttt tcgtatgtaa aagtccccac gagcctcttc attttttgca ttatagcctc
    48541 cgtctggctt ttccttaatt ctcagaacct cataacctaa tgactcagca tattccttaa
    48601 acaacaactc tcccaaagaa ccaaataaat atccctctgc tcctggactc attttcaaaa
    48661 cctctaaaaa tttttgctca tcaattccaa atttattttt aatgtaattt tttaataggt
    48721 tcatatttgc taatctttaa aaaactctaa aatgctaaca ttaagtgcat tagctaattt
    48781 ttcaacaact gtaatggata catttctttc tcccttttct atacttgtaa tatacgttct
    48841 atctaaatct gcagaaaatg caagcgattc ttgtgagaaa cctttttcta acctgagacg
    48901 tttcaaattt ttaccaaatt ttttcttaat atccatgtta caaaactacg attatgacga
    48961 catttgtaca acggactact gtcaacatta tttattgttt ttcaattata tattacttta
    49021 tcaaaacaca attctcccaa aaaaaaacac ccctatttca tcaaacagag gtgtttctca
    49081 ataaatcaaa aaaactattt atttatattg atctaaattt tcattttgat tgtttttctt
    49141 tttactagct cttgctaaga agattattgg tagtataata ataactaaaa aaactaatcc
    49201 taatattaaa cttgatgtat ccataatttc tatatttata atatttaaca tttaaacttg
    49261 acaaacaata cgctgtaagt caataattta taaaattatt aaaacctcta aaaatatttt
    49321 aacaagctat ttaatagaaa taatggaaag gaaaaacctg atgataatct ttaaaaatat
    49381 ttttactata aataaaaatc ttatataaaa cgagatagtt gtcgggaatt atttgtgatt
    49441 ttatttgtaa acaaggcttg gtaattttgt tagaaaacaa aacatttacc tgctttaagt
    49501 ttacaaaatt gtcgtttttg aaattttgct tttgaaaagt aaaattaaaa tactctaaat
    49561 tagaaaagta aacattattc ttaccagatt cttgatttgt cttgatttca atactttggt
    49621 agttgtatat atttgcgttt aatgcaaata ttaaaaaact aagaataatt attatgtaat
    49681 ttttactttt cacaattatt tcccccaaga atctctatct aaacttctgt aatgaattgc
    49741 ttctgcaata tgcgaaggca taatatcttt tgaattttca aggtctgcaa tagtgcgtga
    49801 taattttatt attctatcat aagctcttgc cgacaaatcc aatttttcca tagcattttt
    49861 caacaaactc aaagactcct catctaaagg acaaaattcc tttatcattt ttgatgtcat
    49921 ttgagcattg caataagtgt ttttcttatt tttaaatctt tctgtctgaa tttttcgtgc
    49981 ttgaataact cgcttttgaa tttcagaaga tttttctgct ggttttgttt gtgctaaatt
    50041 ttcaaaattt acaggcgtaa cttcaagatg aatgtcaatt ctatcaagca atggtcctga
    50101 aattttattc aaatattttt gaatggaatt ttgagtgcaa aggcaatctt tttctggatg
    50161 gttataatat ccacaaggac atggattcat tgctgctacg agcattaaac ttgcaggata
    50221 ttccacagta aattttgctc ttgaaattgt aactactctg tcctccaaag gctgacgcat
    50281 aacttctaaa actgtgcgtt taaattcggg aagctcgtct aaaaataaaa ctccattgtg
    50341 agctaaagaa atttctcccg gctgaggata agtaccgccc ccaactaaag ctacatcaga
    50401 aattgtatgg tgaggcgaac gaaaaggacg ttgcaaaaga agtgatttat tggcttcagt
    50461 ttttccagct acagaatgga tttttgtagt ttcaattgcc tcatctaaac ttaatggtgg
    50521 caaaattccc ggcattcgct tagcaagcat agttttccca gaacctggcg gaccaatcaa
    50581 aataatatta tgaccaccgg cagcagcaac ttctaatgct cgtttaacat tttcttgtcc
    50641 ttttacatca ccaaaatcaa tttctaattc tttaacactt ttttgaaact ctgcttcaat
    50701 atcaattctt acaggctcta aatctttgct gtcgttaaga aaacctacta aatccataat
    50761 gtgtttcaca ccataaacat caataccatc aacaacagca gcttcttgag tattttccac
    50821 aggcaaaatc aagcctttaa aaccattttc acgagcattt atagcaatag gcaaagcacc
    50881 tttaattgga tttacatttc catctaaaga caattcgccc atgataatat aatcttctaa
    50941 ctttgaaaaa cttatctgtt cagatgctgc gagaatacct acggcaattg gcaaatcgta
    51001 agcagaacct tcttttcgca aatcggcagg agccatatta attactattt ttttgccggg
    51061 ccacttaaaa ttattggtcg ttaaagcaga aataatcctt tgttgacttt ctttaacagc
    51121 agaatcagcc aaaccaacta aataaaaatt tatacctctc gaaatattaa cttcgatagt
    51181 aattgtttcg gcatttattc cattaacagc tgaaccaaaa gactttacaa gcatctactt
    51241 atctaaaaat atatttgtga gcaaagttaa taatatttta taacttttat atcatttgaa
    51301 aaacaattaa aaaaaagata ttatctttga gtaaaatttt taaggcttat tttatgaaga
    51361 attttgtttt atttattttc tgtttggctt taacatttgc gaatttattt tcgcaagaaa
    51421 aaaaagaata caaaatagct tgtgttactt tttataatgt tgaaaatctt ttcgatacaa
    51481 ttcccggcac aaacgacagt gagtttgctc ctgatgcaaa caaaaaatgg aactcaaaga
    51541 aatactatgc caaactcgat acaatagctt ctgttatttc aaaaattggt aaagatgtaa
    51601 ccaaaacagc accagcaatt gtaggactta gcgaaattga aaatattact gtcttaaaag
    51661 atttggttaa agttaaagat attaaaaact ttaattatca aatcgttcat tttgatggac
    51721 ctgatcgtcg tggagttgac tgcgcacttt tatatcgtcc tgaatacttt aaagttacaa
    51781 atactgctat gcaccatgtt tatacaccag aagatcctga ctttagaaca agagaccaat
    51841 tattggtaag cggtattttt gatggagaag aaatgcactt tatcgttctg cattggccct
    51901 cacgtagtgg tggagaaaaa agaagtgagc caaaaagatt tgcagcagca gatgttactc
    51961 gtagcattgt tgactcaata ttaacaatta atccaaaagc taaaattgtt attatgggcg
    52021 acttaaacga tgaccctgac aataaaagcg taagaaaacg tttacaaaca caatacaaaa
    52081 aagaaaaagc tcagcttcct tacttgtaca atgctacaga acctcttttt agaaatggtg
    52141 taggctcttt ggcatataga gataaatgga atctttttga ccaaattatt attacgccag
    52201 aatttttaga aaaagataaa tcaagctata gattttatca agctcatgtt tttaacaagc
    52261 cttatttaat gcaaaaagaa ggtcgcttta aaggatatcc ttggcgtact tacgttggcg
    52321 acacatatat gggtgggtat agtgaccatt ttccgactta tatttactta ataaaagaga
    52381 aatagtcggc attttataag tgtccaactg ctttacttca acttcgttca gcaaccacgt
    52441 tgctttcgat attcggactc agtcacgtac acttcggctt tgctcagtgc atcgcttctg
    52501 gtacgctcct tcgtcctcaa tctcagacgc cttgcatttg aacacttcta aaacgcctta
    52561 aacttaatgt taaaaatctg attttaagag gaatagagca taagcataca cgctagaatt
    52621 tagaaaacat aaacaaaatt cctaaaactt tcaaaatttg agaactttta ggaatttggt
    52681 gatgtcttta cggataatct ttgcaattta tttctacata tcttctaacg acaggcactt
    52741 agtttcaatc gtatattttg gcagttctat ttgttttaat ctttttactt tttcagcaaa
    52801 catattagga cgaaaacgct ctttgttgcg ttttacctct accgccaatg ctttgttttt
    52861 ttcaaaaagt aatgcaacta tgtctatttc gtttgcctcg cctttagttt cccaccacga
    52921 agctatttca cggtataagc cactttctgc caactttgca cgaaaatatc gctctaacat
    52981 ttttcccgaa aatgttgtat aatcagattt tataatattt tgtaagcctg aaaacgcctt
    53041 tatttctatc atagaacgat tgcgttcaaa ataattgaac caaaattgca agaaattatc
    53101 aattatctca taacgaacag tttgtgatcc ttcttttgca ccaaagggac gcactctttt
    53161 caaaatattg taatcttcta tcaatcgctt aatgtgtcca ccaatacttt tttctccaag
    53221 tgctgtcata atagcaggtt gcgtgttaat tcctccagaa atacaactca atatagaaaa
    53281 ataagtgcca taatttttgc caaattcttc tattaacaag tttttgcctt catcaataaa
    53341 aggagaattt tcgcgcacaa taaattcaat catagaatca actttcaact cgcagccatc
    53401 acaaatgagt tctacatact ttggaatacc tccagtaaaa gtatataacg ccaacaagtc
    53461 atcattctga tagtcaggat tataatcgct gataatggtt ttcaaagtat cagtatcgaa
    53521 aggtgaaagt ttaatagtat tatcggcacg accaaaaagc ggttctttag aattttgaaa
    53581 aattttattc atcaacgaat aaaccgagcc actcacaata agattgactt ttgtttttcg
    53641 gcgatactga tcccaaaggt tttgtatgtc gctatatatc gactcattaa tattaaaaaa
    53701 ctcctgaaat tcgtcaataa tcaaatggaa tgaacgttgc tttccaattt ccataagata
    53761 ctgaaaaaga gagcgaaaac tcgaaatctc ttgtggtaca aacacattca gagagtttga
    53821 aatttcttga atatattcgt tgcaaagagt tgcttcagtt tttctaccaa caaaaagata
    53881 gacgcttggt tcgtcttcaa ctgacttcat tatcaaactt gttttgccaa ttcttcgtct
    53941 gcctgtaact acagtcattc gagaatattc gctaaaagag agatttttaa ttctttgtaa
    54001 ttcagctatt tcttttgttc tatcgtaaaa tttcatattt aaaaacttta gttaccaccg
    54061 ttactgttac ggcagttaca aaattataaa atatttttga aatacaacaa atttttgaaa
    54121 attattttct tctatataag tagaaataat ctaaatttta attatttttt tacgaattgt
    54181 tttaccatcg gtcaatctca cttctaagag gtaaagtcct gattgataat cacatatatt
    54241 tatattagaa ttgtcataaa cgcctttaag ctctccttgt aagttaaaga ttcgtatatt
    54301 ttcaatagct gaattttcag atacatttac tgtgagttta tcaattattg gattaggact
    54361 aattgaaatt ttgtctattg taaattcagg aacattaatt aaatcccagt aatattcaca
    54421 atccttatca gtataattaa ttaaagggaa agagtcgtct ttatagcatg aaaaatttat
    54481 atcatcaagt tcaatagcca agagagaatc acaattgttt tctagtggga atagatatcc
    54541 cgatgatgct ccaaattttt caattaccca accataaaag tatccactag aatttgcatg
    54601 tggcctaaca aacagacaac gtaaaggcat ttcattaata gtaatagttt taacactatc
    54661 tacttcaatt atagatttag aacatagcca ttgattggtg tcaattccta aatcccaagt
    54721 atctcctggc tgagcgccaa aattgtataa aatgttaaac ttattattct tgtatctgaa
    54781 tacagtatca ccagataaat aagtaaattc aggctcaagt gtgttaacac caaaataata
    54841 catcgattat tttgatctac tgaaaataca tacttagatt gttctagttt ttgacaaact
    54901 ttcccatcga taattgtatc tgccacgtaa tttactttgt aaaaaccaag atccgaaaat
    54961 ccataatacc gataatgcca ttctgctcct gcgtcaatcc atgtttgact aaataaaaaa
    55021 gcattgaaaa acattagaat ataaatctct attccttgca ttggaatcaa aagccgaaga
    55081 ctccaccccc tgattttttt tcaccataga ccaaaaagaa gtctgctgaa taatgggagt
    55141 tgaataaaga tcaacaatct cttttttcag aatactaagt gtcattatta gaaataaaat
    55201 ttttgcaaag ttacccctaa taaaacttct aaccaaatta agagaagttc aaaaaaataa
    55261 atatttatac attttgtttg gtagattgaa ataaagtttg taggtttata ggcgtaatat
    55321 tctcataaat ttaatttgag tatggaaata ttgattacaa ttattttaat cctttctatt
    55381 ctgattatct tttcaacaaa aaaagcagaa aggtttttgt attctcatat tctccccttc
    55441 ctgaccaaga ttaaattctc agacaaatac aagaatcata attattcaaa attcaaccca
    55501 aatcttgatt tagatgttct tcgcaatcaa tacttacgca cacttttgcg agaacgtcat
    55561 ttcttacagc gttgcgttac gggcaaaagt gcttgtgttt gtaaccaaat aaatacactg
    55621 tatgtgtaag aaagcacagc gtctttggaa ggataaggtt tgtaaactcc tttgttgaga
    55681 cgaagtcaac gattgaaata tcataacacg atataactaa aaatctacaa gccactattt
    55741 aagtattctg tccttgatgt aagaatagtg tgtgttaata ttaaggacaa aaaaataaaa
    55801 agaaaaatat tatggcaaga gaaccgagaa aagaaattaa agtagaaaga gcaaaaacaa
    55861 caggttggtg gacttgggaa gctaactggt atgatgaaaa tggtgaatgg atgtgcggga
    55921 caggaggaca tggctcaagc aaaagtgatg ccaaaagaca agggaaaaat gcagaatgtc
    55981 cttgttgtga cagctcaaaa ttagcagcta aaatagagaa tgttaaaaaa atattcattg
    56041 aacctcctct tgtagatgct gaagtagtta atggctattt atgtaatttc agtatgtata
    56101 atcctgaagg agagaagata aaattttcaa cttctgaaat ctccgataat actgcttatt
    56161 ggatatttgg gttggattgt tttgaaccta acctatctga aaggagcgaa ttagtcatag
    56221 ctatgctaaa aatatgggga gtatacagaa gagggaaaga caaaaaggca ataatggaaa
    56281 tacataatct tactgaagaa tattttgata ttattgcaga taaatcatgg tcaaacataa
    56341 atataaaatt cgatgaagaa gataacatgt attgggatat tattgtttag tatcacaact
    56401 ctgattggta attttgcaat tgctcaaaag attccttata tgactcaaat agatagtaag
    56461 gaaatgaaat atattgacaa tacagaggtt tctatcagtg attggcggga atatgaattt
    56521 gatatgaaaa accgttttgg tagtaattct caagaatttc tttcaacaat tccagatact
    56581 gcattattta gaaggtatta taattattct tttattcacc caaatgcaaa caatatggag
    56641 aaacaaaatt tttcaaatgc agatgtaggt tttatgaaga ataattatga taaatatcca
    56701 atgataggtg tttcttatga gcaatgtata gattattgca agtggagaac tgaacgattt
    56761 aattggaaaa taaaaggagt aaaacaaata gagttctctt tacctagccc agaggattat
    56821 aagaaagctg aatcctatgc agagataact ttcaatcctc ctttgtcaat tcttaaagat
    56881 aaaaaatctg ggaagccaca gggaataacg gataatgtta tagaatacac tttgggaaat
    56941 aaagaccaag ttctcgatag acctataggc tttaggtgca ttgctaaaat aattaaatag
    57001 aaaaccatat ttcaaaattc aaaataggag ttcaaaatta attgaactcc tattttgttt
    57061 ctatcttgtt aagtatgttc ctatcctaag aatctaaccg attattaaaa tatttaacaa
    57121 tcaataaaca agtatttgat gtttggggta tgattatttg atgccgaagg tatcagaatc
    57181 aaatggtttc gcctgatttt tcttcaccat agaccaaaac gaggtctgct gaataatggg
    57241 agttgaatat aggtcaacaa tctctttttt cagaatacta agtgtcatta taacaaataa
    57301 aaaatttttg caaagttacc cctaataaaa ctcctaacca aataaagata aattcaaaaa
    57361 aataaatatt tatacatttt attttgtaga ttaaaataaa gtttgtatgt tttgttatgt
    57421 aattaaaaaa atgatacgaa atgagctaaa aatatacgat agtgatacga aaatttccac
    57481 gctcaataaa attacaaact aagcccgcaa aacaaattca gcggagttaa atctggcgtg
    57541 cttaggagtt tcatttccca ttttgttgcc aattgaaatt acttgaccat tcataaaagt
    57601 tgttaatatc tttttcatgt ttttttagcc acttgtcaac atctggatag ccagaagttg
    57661 cgaatgctat gtgagcaaat gtttttataa attttccatc ttgcatttct ataaaatatg
    57721 gcacataata atcccaataa aaaccataat tttcattttt cgattctttt aatgaagaac
    57781 aaacaagttc aattttttcg ataaattttt taacctctgt tttcttttta ggtttcttgt
    57841 cgtagtccaa tgcggtttcc attgttaaaa gcaagtctgt agttgcaaaa ttattttctt
    57901 taatttttca gtcttcagtt gtgtcgctaa gcatattggg atttatgttg acattaatag
    57961 tgtttttccc tgttttttct acatttgctt tcattacgta ttgtaataat tccaaattat
    58021 cttttgctct attcgtttgt ggttctaaaa ttagaaatct gctaagggct aaaattgcag
    58081 gaattctgtt ttctgataca cagagtaatc tacccaaaga attgtgagaa ctcgcatgtt
    58141 gtgggttgaa agatacggat ttttgactac aaataattgc ttcttcatat ctttcaatat
    58201 ttgataatgt tatagctttg ttgaaatata acaggaagaa gtcgggaaat agttttaacc
    58261 cttcatcata catttcaagc gacttgtcgg tttcctttaa tgcgtcataa gcgtttccac
    58321 atgttacata aactaatttt agaccatcgt ctccttgatg tttttcaatt gctcttttac
    58381 aatttttaat agcgtcatcg tatttctgta aatacattaa agacattgct ttttcagcca
    58441 atgctaataa attgtcttca tcaagttgca atacttgatc atatttatca attgcacttt
    58501 tatagtcgcc tttgtcgtga aaataaacac cttctccaat gagtttttca atttcttctt
    58561 gctgtccaaa taaagtgcta catagcaaag aaaacaccaa taataaaatt aactttttca
    58621 tatttactgt ttttatttaa gcattatcgc aaatcacttt tttataaaat tttcaaacta
    58681 taatgtttac tctttaatta tctttccagt aaatacattt ccatcttcaa agtctatttt
    58741 atataaatac atacctgcac ttaaatcttt cacaacaatc tcagaacttt gatagatttt
    58801 agttgacaaa acttcttttc catatagatt aaatagtttt aattgaccat aaccaccatc
    58861 aaaattatct atataaatta catctctaaa tggatttggg aacaggctaa tactttttat
    58921 ttcatcatct acatttgtta catgtgaaca cttattagta cctatgaatt cattatttga
    58981 taagtattca ccccaccatg ggcatccatt atatacatca acatattcta tcggaacata
    59041 tatagttagg gatattcgtt aaattcaaaa ttttaatttt gtcatttatt tattttgttt
//
LOCUS       DAOPNX010000004        54678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_770_length_54678_cov_7.315581, whole genome shotgun sequence.
ACCESSION   DAOPNX010000004 DAOPNX010000000
VERSION     DAOPNX010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 54678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54678
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_770_length_54678_cov_7.315581"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PKN53_00815"
     CDS             <1..604
                     /locus_tag="PKN53_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87528.1"
                     /translation="ITGKGYKIVDRQDLITFFSNNVVDNDIYVDFVENINSLESSIKS
                     FETLPIKNWDYYSWQGFYQFLDSQIKVVDWQYVSNPAGGFLGLWWHFIDWEGYKVYLQ
                     IEQDNFCFKIGPVEEKRSAVRNKWYSVLSKYIESKKMYEIKKPTRFGNGTYMTVGVVS
                     REDWLGKDNAILDKDVVIRNLKKYEKFLSNCVRNSKRNVK"
     gene            856..2553
                     /gene="ftcD"
                     /locus_tag="PKN53_00820"
     CDS             856..2553
                     /gene="ftcD"
                     /locus_tag="PKN53_00820"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOD87529.1"
                     /translation="MKQIIECVPNFSEGRDMSIIKQITDVITAIDGVKLLDVDPGAAT
                     NRTVVTFVGTPNEVLEAAFQAVKKASQIIDMSKHKGAHPRFGATDVCPLIPISNISME
                     ETAELARKLAKRIGEELNIPVYCYENAAFTEKRRNLANCRSGEYEGLAKKITSQEWKP
                     DFGSNVWDAHTMRTGATAVGARNFLVAYNINLNTTSVFKANSVAFDVREKGRVKREGH
                     PVTGKPVLDKDGNKVYEPGLLKSVKAIGWYIEEYGVAQISMNLTDISVTPLHVAFDTV
                     CERATANGLRVTGSELVGVMPLKAILDAGKYFLKKQGRSTGISDAEIIKIAVKSLGLD
                     ELKPFDPKKNIIEYMLEDDNNKKLVDMNLVDFCHLAGSEAPAPGGGSVSAYIGALGAA
                     LGTMVANLSANKYRHDDQWENFSDWADKGKAYHDELLKLVDEDTVAYSRIIESYGLPK
                     GNDEEKALRSQAIQDATKYAIEVPFKIMKVCYNSMEVMLEMAKTGLEASVSDAAIGAI
                     AARTGVQGGFLNVKINAPSYKDKSFVDKVIAEGNEIERKAIALEKEIMEIALAKINK"
     gene            2720..3340
                     /locus_tag="PKN53_00825"
     CDS             2720..3340
                     /locus_tag="PKN53_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87530.1"
                     /translation="MDFWQLRNQFYNLVCFNINQVYAWRPNFEKTNLTRWVKQNYIIK
                     LRNSWYSFPEYLETPNIQHFISNKIYSPSYISLHSALAFYGIIPEAIVRTTAVSSLKK
                     MSFENAFGSFSYQQILPELMFGSEQKIFLNKHSLIFASPEKAILDLLYLYPQYTTEQE
                     IRDLRFDEEFMQEDLNLERLNEFTERFQSKILRNRVNTLLKIYDLC"
     gene            3334..4119
                     /locus_tag="PKN53_00830"
     CDS             3334..4119
                     /locus_tag="PKN53_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOD87531.1"
                     /translation="MLNFEQIKNYFPPQIRENPAHNKYILKEYIQLMILNFLSSSKYV
                     KKIAFIGDTNLRLLKGIDRFSEDLDFDCKDFSIEEFVEMSQSVLTFLKRSGIKAETRE
                     KQSDKLTAFRSRFYFPELLFELGLSAYKEERFLIKIESQNQKFEYTPQMATISGCGFF
                     MSFPVPPDDILCAMKISALLSRAKGRDFYDVMFLLSQTNPNYDYLAQKQGLHNLMELK
                     IKLEETINKVDLHNKSKDFEHLLFNKENKNRILHFKEFVEKLN"
     gene            complement(4417..6216)
                     /locus_tag="PKN53_00835"
     CDS             complement(4417..6216)
                     /locus_tag="PKN53_00835"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HOD87532.1"
                     /translation="MRLVCLFISLFLYVVAFSQTNSGKLIVLHSNDLHSNLTGFSPES
                     EYTPCSVDDDDTRGGFARIAALIDAEKKANPDNVLVVDAGDFLMGTFFHIFEVNDGFQ
                     LHLMKKMGYDVVSIGNHEFDFGPNAFAQIVNKNLEKGEIPQLVLSNAELSKKSDLDDN
                     LEKLFEKGIIKPYTIVEKAGLKIGVFGIMGDDANEVAPNAKPVIIQNRIKISKQIVKT
                     LRYQEKVDVVICLSHCGVTKDKNGNWTGEDVELALKVKGIDLIVSGHTHTEIHEPIIV
                     NNTYIVQTGAQGQNLGRFEMNVESGKVKSARFQLLAVDDKIYGDCKIHQIISNRIKLI
                     DDSILRPLGLGYFRPLAETDYQLGCDREEELSKSNLGPLVADAIHYYIHNFSNQKADI
                     SLVAAGVIRDKFRVGKNGIQTAVDVFRIMSLGEGDDSMPGYPLAKVYLTAKEIKNLFE
                     VLLLAPKMSPSYLCYFSGAKVYYNSEKGVLKKVVKIELDGKEIDISKKNKNLYSLAAN
                     SYMLEFVGKVRGMTMGLVKIVPKNAQGEKITNNKTTWIDFDENKPGIQEGKEWIALVK
                     FLQSFPDTDNNKIPNFPEKYKTAIVNTIDVATK"
     gene            6467..7309
                     /locus_tag="PKN53_00840"
     CDS             6467..7309
                     /locus_tag="PKN53_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOD87533.1"
                     /translation="MEDKKNQDILVYTDFTEVGEKSVLWASFLARKFKRNLQIIHVIN
                     ENSLIYFSKDDTYDEAKEALAALCEDISSTHGIKCKYIVEEGCTCTIINSNAERIDAF
                     FVVLGTHGKNDPQFLSGSSAIKIIRKARIPYFVVQKNSPLPDSKKRFVLPMNFKKEAK
                     QKVGWTTYFALNLKLEIDLFYYENPQDNLKKNIHFTNTFFNNYSFKYEHKPQPKKYKS
                     IEKLAVDYADEIDAFCICLLISSKENLVTKIFGFPETSIITNSAGIPVLCVNPKKDLY
                     IPCI"
     gene            7428..8000
                     /locus_tag="PKN53_00845"
     CDS             7428..8000
                     /locus_tag="PKN53_00845"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOD87534.1"
                     /translation="MIKLKQLPYEMNALEPYISAKTVEFHYTKHHQGYVNNLNNLIEG
                     TEFAGKDLEEIIKTSEGPIFNNAAQVWNHTFYWEGFSADPQKEPKGKLAELINAKWGS
                     FEKFKEEFTKTATGLFGSAWAWLVIDPKGELKIVGTSNAATPLTTNDKALLTADVWEH
                     AYYLDYQNLRATYLKSFWELVDWKKVEARM"
     gene            complement(8018..10639)
                     /gene="mutS"
                     /locus_tag="PKN53_00850"
     CDS             complement(8018..10639)
                     /gene="mutS"
                     /locus_tag="PKN53_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOD87535.1"
                     /translation="MKKSAETPLMKQYYSIKAKHPDAILLFRVGDFYETFEDDAIKTS
                     SILDITLTKRANGTASHIDLAGFPHHALDNYLPKLVRAGQRVAICEQLEDPKMAKTIV
                     KRGITELVTPGLSLNDNVLERKENNFLASIYIGKQFIGIAFLDISTGEFFVAQGQANY
                     IDKLLQSFKPKEVIYEKNKKKKFEENFSGNFKDFGFDDWVFTIEHAKEKLNKQFGTAN
                     LKGFGISNLESAIIAAGAIIEYLDFTHHTAREHIASISRIDESNYVWLDKFTIRNLEL
                     FGSSNEEGKSLIEILDNTLSPMGGRLIKRWLSLPLKNLDAINRRLDVVEFFINNPDFF
                     TKLKEQLHQISDIERLSSKIAVLRVNPRELLSLRNSLVAIQPIAEMFKNSDNKIIQNL
                     GENIELCDELVKKISLEINPECPALLNRGNVIAQGVSNELDELRSLASGGKEYLENLE
                     KSEAERTGITSLKIGFNNVFGYFFEVRNTHKDKVPEEWVRKQTLVSAERYINEELKLY
                     EEKIMGAEERIQSIENQIFNDLVLFAGEYVGLIQKTAGIIAEIDCLHSYAVTAIQNNY
                     CKPKLNESNVLDIKDGRHPVIEKMLDFGEEYVPNDVYLDDKTTQIMMITGPNMSGKSA
                     LLRQTALICIMAQIGSYVPASSAEIGIVDKIFTRVGASDNISMGESTFMVEMLEAASI
                     MNNLSPKSLILLDELGRGTSTYDGISIAWAIAEYIHEHPTAKAKTLFATHYHELNEME
                     KSFKRIKNFNVSVQESKDKVIFLRKLKEGGSEHSFGIHVAQMAGMPKSVVYRAKKILE
                     QLEGKSKEMNKGVNLNKPVEKIATSREGYQLSFFQLDDPVLSQIKKELQGIDIDSLTP
                     LEALNKLNEIKKLSGSK"
     gene            11248..11937
                     /locus_tag="PKN53_00855"
     CDS             11248..11937
                     /locus_tag="PKN53_00855"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD87536.1"
                     /translation="MAKRNKKAAEKDGFKSVESAFSRAENFIQTNQKAFIYGLAGVLV
                     VVLGIIGYFKLIREPHVKEAYSVSFMAERYFVQDSFNLALNGDGDNLGFIDIVDEYNN
                     TPMGNAAKYYAGVCFMRLKDFESAIEYLEKFKSKDPMVGALAKSLIGDANLELGNIDA
                     ALDNYLKAIEQADNELLSPMLLMKAGRTYELQKDYAKALEIYKRIDTEYYGTSEHRNI
                     EKYIKRCEMNL"
     gene            12001..12549
                     /gene="ribH"
                     /locus_tag="PKN53_00860"
     CDS             12001..12549
                     /gene="ribH"
                     /locus_tag="PKN53_00860"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOD87537.1"
                     /translation="MASSLKNLSQYSEQGIAKNTKKNPLKIGIAVSEWNQEVTASLAE
                     GAIETLKKYGFSDKNIVLKSAPGSFELPLLARYFIEHANIDAVICLGCVIQGETRHFD
                     YVCSGVTQGIMQVNLEYNIPVAFGVLTTDTLQQALDRAGGKHGNKGVEAAIAALKMLQ
                     IKLDFEEEDELRFQEMNRSFFE"
     gene            12542..13117
                     /locus_tag="PKN53_00865"
     CDS             12542..13117
                     /locus_tag="PKN53_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87538.1"
                     /translation="MNKKIKILISILNIVLIFTLFSCSFSKEWLEAEVTYTNQETESL
                     LVKPNPFSYYTFIRSKKTIDEKTKVLIPENVYKIKGKDFAYVAMFFDEANYGMESWSF
                     GKVLAGDNIMLVETKYQAKTCACNTSGKFFKDYFLVYGNYFLKIKTYKKTNICDKQEI
                     YSFLDTYYKNSYNKEFNNIEDLTKLIRDINN"
     gene            13257..14057
                     /locus_tag="PKN53_00870"
     CDS             13257..14057
                     /locus_tag="PKN53_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD87539.1"
                     /translation="MGKIIVLANQKGGVGKTTTAINLAASLAVLEYKVLVVDADPQAN
                     ATSGLGFDVRHIKTGLYETLIKDEDPRGLILSCNIPGLFLLPSHIDLVGAEIEILDLP
                     NREKILKHVLEKVKDDYDFILIDCSPSLGLIVVNALTAADSVLIPVQCEYFALEGLSK
                     LLNTIRIIQEHLNPDLDIEGFVMTMYDSRLRLSDQIYEEVRKHFDTMVFETVIHRNVK
                     LGEAPSFGQTIFDYDVTSKGATNYLNLARELLQRNNMTKIKNEEKILE"
     gene            14059..14940
                     /locus_tag="PKN53_00875"
     CDS             14059..14940
                     /locus_tag="PKN53_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOD87540.1"
                     /translation="MAKKNALGRGLNALIESGDYENLNKTREPNEIKIDLIEANPFQP
                     RSNFDEEALQELADSIKELGVIQPITVRALENGKYQLISGERRLRASKLVGLEKIPAY
                     IRNTDDQGMLEMALVENIQREDLNAIEVAISYQRLMDECNLTQDMLSQRVGKKRSTVA
                     NYTRLLNLPAKIQLGIKEDKISMGHARALLAVKDTDTQLMVYDQILKYDFSVRKVEEI
                     VKELSNEEKVKEPAKPKKTPKTTEDYLQLQKHLSSCFAADVEFKRSAKGSGKIVIPFK
                     NDYDLERIIALLDKLNV"
     gene            14944..15483
                     /locus_tag="PKN53_00880"
     CDS             14944..15483
                     /locus_tag="PKN53_00880"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOD87541.1"
                     /translation="MNCKTLIYIIFILFFFPNLIFAQNIEANKSKPKHSPRKASIMSA
                     CLPGLGQAYNKKYWKIPIVYAGIGGFGYLAINNQIQFNKYKNAYKLLENGQANDYPDF
                     NQEALVHMMDGYRKKRDLCILGTVAFYVLQIVDASVDANLFDFDVDDNLSVSIKPQLQ
                     SDFTNSTIIPGLSCSINIK"
     gene            15483..17024
                     /locus_tag="PKN53_00885"
     CDS             15483..17024
                     /locus_tag="PKN53_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOD87542.1"
                     /translation="MKKVLSILIILLSQASFAQQDEDTVFQHRKNDGYSMADHLDSLL
                     ASWYYYTGRDTIAYLSLTERHTHHYLDIPDSIFEKRIRKIVTPIELTYNKNVQKYLDK
                     YVKKGKWIAPKFLGLSLQYFSLFEEKLDAYDIPLELKYLPVIESALNPKAKSRAGAVG
                     LWQFMYKTGLGMGLEINSYVDERMDPEKSTDAACRYLKSLHAMYDNWTLALAAYNAGP
                     GTINNAIRRSGGKTNYWELYPYLPNETRNYVPGFTAIVYLFTYADKHGYKPEYIEFIE
                     DFDTVMVRKELHFAQLDSVLGISVGESRELNPQYKLDIVPAKNKEYPLKIRRKYLSKF
                     MELEDSIYNFMDTLFFNPKTPVVISDKYVETIVPATQPEGTVALQYTVKSGDAVGLIS
                     NWYGISINQLKAWNGLASNNIRVGQVLKIYVPAALESKYKIVNSLTFEEKQRMAGVNT
                     SSTPVKEEPIDPAWEYYTVKSGDTPYGISLKYPGVSVDDIMKNNNITNASGLRIGQKL
                     KIRKK"
     gene            17037..18011
                     /locus_tag="PKN53_00890"
     CDS             17037..18011
                     /locus_tag="PKN53_00890"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002527.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOD87543.1"
                     /translation="MRNIAVLAGGYTSERHISLNSGKQVLESLDKTLYNAFLVEVNVD
                     GLFCIDKEKKYQVNLNDFSCALPEKTVKFDFAYIVLHGSPGEDGKIQGYLDILNIPYS
                     SCSVYASAITFNKLASKKLLENTGVHLAKSMRIEQGMSFCLDELVDEIGLPCFVKPNT
                     AGSSYGITKVYEKDKLIDAINHAAEEDSTIIVEEFIKGVEVSCGVFKTKNQEIVFPVT
                     EISTKNDFFDTEAKYQPGLTDEITPARISDEETKAVQDFTSLIYDILELSGIVRIDYI
                     VKDKKPYFLEVNSIPGMSAESIIPKQIRAINKTVTEILSLVIESKFED"
     gene            complement(18012..18620)
                     /locus_tag="PKN53_00895"
     CDS             complement(18012..18620)
                     /locus_tag="PKN53_00895"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HOD87544.1"
                     /translation="MKIKFIALFLIFLGISFLEANAQDKGKPKYYHKELNVDSIAKQI
                     SDPLYVFPQVNIYPQKAFKNARERRRYDKLVNNFKKVYPYVLELSATYNNIEDSLKNF
                     PNDKYREVYVKGREKQLMKYYRPILSRFTLSQGVLMVKLLNRESGSTAYDIIKELKGN
                     VNAFFWQTFAVLFGNNLKREYDAAGKDYDIEILVMRYHDGYL"
     gene            18788..19489
                     /locus_tag="PKN53_00900"
     CDS             18788..19489
                     /locus_tag="PKN53_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOD87545.1"
                     /translation="MKNEIKSIEVLFNSPDSVFSKLVKADKEYLLANTEYRVYKKNEI
                     VFSENTKPTGLLCLSEGKVKIYKEGAGGRDQIIRLSRPGGFIGYRALFAEQNYSASAA
                     AIENSVAFIIEKKALFSIMKRDPNLTFGILKSLATELGVSNMRTVSLTQKHIRGRLAE
                     SLLFLIDTYGYYDDGTTIKALLSREDLANLSNMTTSNAIRTLSSFAQEKVIALDGRKI
                     KVLDLQELEKISDMG"
     gene            19597..22011
                     /locus_tag="PKN53_00905"
     CDS             19597..22011
                     /locus_tag="PKN53_00905"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOD87546.1"
                     /translation="MISQECKGEAAKLLVEDLKFSNNFEYINTLLNETDEMMSICMLK
                     DNYPAQNYPEIGIAINKLKIEGTSLQLFEIINIRQVLEQAKSLKHFFKLDEKNQYPFL
                     NKIVNQISFPNYLYDRINSVVSKNGTIKDSASQNLKEIRKSLNDTQTNITKKLNNVLS
                     AFKKEGWVSKDLSATYVNGRLVLPIETIHKRKIKGLIHDESASGKTSYIEPQEVVELN
                     NTLRELEIAENREIQKILFELTDDFKPYREELLTLYENIAYLDFVWAKAKFSLRIEAI
                     VPKIDKDPVLDLHKAKHPLLFLNFKKEKKEVVPSSFYLNSENRILLISGPNAGGKSVC
                     LKATALLTYMNQCGLPTSVGGNTVMGIFDKIFLDIGDQQSIENDLSTYSSHLLNMKYF
                     LKNSDSRTLILIDEFGSGTDPILGGTLAEAILEELNTKNVFGLITTHYSNLKIFASNT
                     PGIFNGAMLIDQNKMEPTFILETGIPGSSYAIEIASRIGLDQKIIENTKLKAGDEQIN
                     IDKFLRRLIRDKKYWEKKRQKIRIQEKKIEELVEKNIEELEKLKTSRRQILDSAKKQS
                     EQMLADVNRQIENTIRQIKESNADKEISKKARQEIEEFKEDFKNSLSKEQDEIERKYN
                     HYKKKQKEIEAKKAATQKAKPEIIKIDENKINIGSKVRIKGQEVIGEVIDLGEKNAVV
                     SFGNMYSSIAFEKLEKVSETLYKQQNRSASNLHFNYNEKVLNFKPYIDVRGDRVEECL
                     KKISNLIDEAVMLSFKEIKILHGRGNGILRQYIRDYLRTLPEVESFHNEDIRFGGDGI
                     TIVKLR"
     gene            22012..22596
                     /locus_tag="PKN53_00910"
     CDS             22012..22596
                     /locus_tag="PKN53_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOD87547.1"
                     /translation="MIDIEDIIVHYDIFKECFCCDLEKCKGICCVEGDSGAPIEVEEE
                     IKIKQILPIIIDELTDEAKKTIEEHGVSMVDIDGDLTTSICGNTGPCVFIQTDENGIA
                     YCVIEKYWEKGLIDFRKPISCHLYPLRISKYSTFEALNYHMWDICADARVLGKKLNLK
                     IFQFLKEPLIRKYGEEWYEDLLVAAKLFEEENKE"
     gene            22657..22729
                     /locus_tag="PKN53_00915"
     tRNA            22657..22729
                     /locus_tag="PKN53_00915"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22690..22692,aa:Lys,seq:ctt)
     gene            complement(22793..24349)
                     /locus_tag="PKN53_00920"
     CDS             complement(22793..24349)
                     /locus_tag="PKN53_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87548.1"
                     /translation="MKKFVLILLALIAFAKLTNAQVSTIFGYDKEIVLPAMNEYVRLI
                     GADSDGFYALRIDEKDALHLDFYNNTSMSRENSNQLILPMLGGMQSEYVEMFYLDSKL
                     ILFFQVVDNTRKEKTLYIQHVNRTGQVIGEATAIGKLTNQNMSVDFHVELMDNQQNIF
                     VYYHRPFQTYNEEPFFFKVYDPDMREIYNNIVKLPLVNQAFEIEQIKIANSGNVYMLA
                     RVSPDPRQLKRMKTIIYDYKLLYFNVSAGTVKDVDIKGKKHVLVDAIIGVDEEENIDI
                     FGFMVRKGKTDYEGIFHQKYNPTTETFVTGDSKKADYVFSKTEVPEFRAPHLAKNYSE
                     LYNYKLIDVLHLSNGGSAVIAEHQNHWVDSIVVPGTKEILYNDYYRFNDVLVAYCSPE
                     NNMEWMTRIPKTQYSYNDLGQFSSIASFAIGEKIFVFYNDNAKNVKLLQNSQLTGNDL
                     KEIASPSRRGVAVAVSIFSDGKMHGDYLFTKKNKKFRIMPEFFKDFNGRHYIYTQSGT
                     KVKFGMFVGR"
     gene            complement(24390..25442)
                     /gene="rlmN"
                     /locus_tag="PKN53_00925"
     CDS             complement(24390..25442)
                     /gene="rlmN"
                     /locus_tag="PKN53_00925"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOD87549.1"
                     /translation="MSSKINIRALNHEQMIANFANLGEPKFRANQVYEWLWYKSASNF
                     EEMTNLPKAFRDFLDENFFIDFLEIIFKQQSKDKTVKFLFQTADSHNFEGVLIPNKDR
                     VTACISTQVGCPLNCSFCATGKLGFKRNLSAGEIFEQIFQLNKMSEQIFNRKLSNIVV
                     MGMGEPLLNYENTIKALTHISHENSLHFSPQRITLSTAGEAQGIKKLADDNLKINLSI
                     SLHSADNEKRNLIMPINKKYDLDTLKNAISYYYQKTKKRITYEYLLLGGLNDSLEDAK
                     KLTDFTKISPCKINLIEYNPSSESKFTASNKKNTEDFINFIENKNLIVTLRKSKGKDI
                     SAACGQLANKNKNFNN"
     gene            complement(25716..28283)
                     /gene="clpB"
                     /locus_tag="PKN53_00930"
     CDS             complement(25716..28283)
                     /gene="clpB"
                     /locus_tag="PKN53_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOD87550.1"
                     /translation="MNLNDYTIKAQSLIQTSIIKAKELKNQAIEPGHLLLALLNDDDN
                     IFSIILSKLEIDKHNFESAVLSIVNSYPKVEGGEVYLASNTQDVFNLANKHKIEMGDK
                     FISIEHLILALSESKDIIGQMMRDAGVKKDFLKKAISDLRAGSKVNSQSAEDSYDALN
                     RYAVNLIERAQQGKLDPVIGRDDEIRRVLQILSRRTKNNPLLIGEPGVGKTAIAEGLA
                     YRILKGDVPDNLKSKKVFSLDMGLLIAGAKYQGEFEERLKSVIKEVVAADGEIILFID
                     EIHTLVGAGQTQGAMDAANILKPALARGELKAIGATTLNEYQKYFEKDKALERRFQTV
                     MIDEPDIPSSVSILRGLKERYENHHKVRILDEAIVAAVELSHRYITERYLPDKAIDLI
                     DEAASKLRLEVNSVPEEIDVLNRKIQQLEIEKEAIKRENDTKKLEEIATQLSDLNEER
                     NVLTAKWQEEKSLIDKIQAEKQNIENYKIQAEQAERSGDYGKVAEFRYGKITESQQNI
                     EKLKTKLEELQGGHALVDEVVGAEDIADVVCKWTGIPVSKMVLSEREKLMNLEEELHK
                     RVIGQEEAITAVADAIRRSRAGLQDAGKPIGSFIFLGSTGVGKTELAKALAEFMFDDE
                     SMMTRIDMSEYQEKHSVSRLVGAPPGYVGYDEGGQLTEAVRRKPYSVVLLDEIEKAHP
                     DVFNILLQVLDDGRLTDNKGRTVNFKNTIIIMTSNIGSQFLQEQIKSGNENYKETEAQ
                     VLEALKAHVRPEFLNRIDEIIMFTPLSLEQIYEIVKLQLSKLENKLTEAGYELEISED
                     SIKTIAKLSYDPQYGARPVKRFIQKELENQIAKFIISGTSDSKKIKVEFQGDELVIN"
     gene            28613..32566
                     /locus_tag="PKN53_00935"
     CDS             28613..32566
                     /locus_tag="PKN53_00935"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOD87551.1"
                     /translation="MRKILSTFIIILTLGFFNNIFAQANCGAAVAIPLEVYGSCGDMF
                     FTNVDFTAATTSTDAPAPTCGGFNTATEDMWYSFEVPAGVTELAFHAFNSPVPLPMVS
                     PACAPGMAVYRGTCGNLTLIDCFNADAGFMGISNGEIRWEVLNVTPGETIYVRLWEKT
                     NNETSIFFAASVITSLPEADCNNPPELGTTGCNILAPSGTIQAPDDCGWTTTDNVVFY
                     SFEVLPTDPQPVVIEIEYGQCWGNETGGLLPSNPEIQFAVYSWNGVNCNGIGGGPTSD
                     PANTTTYYGCENGVGTVVYSNTLPPGQYVLAMDGHSLPSGNSLCTFGIAASFLDEPPI
                     TGELSVSLSKVNVGCGQLGSATITINSACSTNPTINWSTSATGTTVNNLTVGAYSVTV
                     SDQAPCTDTIINFNIADQSNFAVAITTSGNPCTGPVTLLANVMGANPANVDFVWSPGG
                     QTTQSITVNNGGTYTVTATYGTCVDDASTTVTDGDFDFSVNYTPTFCAGSSGAAQFNL
                     ISGTGPFMYEWSTGAISPGIQITEPGNYCLTATDLNNGCSLTKCFNVIELPSVDVSIV
                     SKDITCHNANDGEVTAIASGGTSPYSYEWNGYIPGETMTNLGAGNYSVVVTDAAGCTG
                     YATTFVNNPPQFTYSISPNQGICYGEQAEISVIANGGVEPYTYVWSDEPTNNIDTRIV
                     TPDTTTTYWVTVYDANNCMYPPQQTKVIVSLPRNISVIVDNVLCHGVCSGSAVIEITG
                     GVPPFNISWTSSDDSDISYSHTIPNRTDSWASLCAGNYAVSLTDKYECEGSAVFAITE
                     PDTMYISTLSGNATCFGYADGFVQVEATGGVPYTNEFGSFYEYHWSNGFSADSLAVGY
                     GIYYVTVNDANGCSHVGMAFVDQPDDIYITAPWNGQICIGEPFSTHVAATGGTGPYEF
                     IWYGSDNSIWYGDGLNVSPEVTTTYQLVTIDSRGCSGPVQTVVVKVHLPIKINEISSS
                     KDYICLGDNLTVEIEYEGGNGGPYTITSPQQGIVNTPYTFSPDSTGYYSFTVADDCGS
                     PVDTDSIYVVVHKAPRVAFFADKTSSCPPGTFHFTNTNEDLNNTYLWDFGDGGFSVEK
                     NPVHTYDKTGNYTVSLTAWTEFGCKKTKTYNNLIFIHPKPRAEFTASPELVSILNAEI
                     TFDNFTEGGTSYFWDFGDGETPDAWSEDKEPKKYTYRSVGEFDITLITKNQFECTDSI
                     HKKIRVHDEFTFYAPEAFTPNGDGINDYFHLIGHGIDKKQFYMVIYDRFGIKVFETNI
                     WDEENPYRMAWDGTHNGSVENGDKILPNGMYRWYASFVDFTGKPHEESGTVTIIR"
     gene            32623..34098
                     /locus_tag="PKN53_00940"
     CDS             32623..34098
                     /locus_tag="PKN53_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87552.1"
                     /translation="MIKKIALILSIIVSFTFVWLINISCEKNNFTTDPEDKLIFSKDT
                     VQFDTVFTQKGSATRYFVIKNSNKQKPILINKIFLAGGDHSPYRINIDGFPVNNLNNY
                     ELERGDSLFIFVEVTINPGQNDMVEEDSIVFVSNGNVQNVKLIAFGQNVTLLNSKFIS
                     NDTTWTDAKPVLIYNSALVEENVTLQVTPGAKVYFHRGASLFVKGSIKVNGDKDNRVL
                     FTGDRLEPYYAELAGQWGAFLEDAHRNTIGIFGGIHLLAGSKNSEINFADIKNAIIGL
                     QVDSCVTPGTPTVRLNSTNIENSKIIGLYALGSTIEAENCVFANSGQYNVACLIGGDY
                     RFLHCTMANYWLENRQTPQLILNNYYNYRDANGNIQTSFRDLHDAYFGNCIIYGSKDN
                     EIKLDFDNRSLYNFRFDNCLIKQKDFKDFEGTDFFIDNIWNENPKFKDIIKPFDYELD
                     TLSPAKDVGKLSIANQVPLDQLGNNRLLDGKPDLGAYERQE"
     gene            34163..35260
                     /locus_tag="PKN53_00945"
     CDS             34163..35260
                     /locus_tag="PKN53_00945"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOD87553.1"
                     /translation="MKNLCLLIVLSFVFLLIGCKSTKDTDQARKTYNPENVNSDFRIM
                     FYNVENLFDTENDPYKNDDEFTPDTGRYWTKTRYNEKLRNIYKVIVAVGGWDLPQIVG
                     LSEVENLKVLKDLINYTPLYKSDYKIIHYDSPDARGIDVALLYRENYFSVISHKPIPV
                     VWPKHIGTGTTRDILYVCGVTNLEDTLHVFVNHWPSRWGGQIETEEKRMHVAKLLKHS
                     TDSIFKINKKANIVVMGDLNDHPTDRSLTESLQAQTNFDKIKSNKLYNLSYYLQVEKK
                     LGTHKHQDQWGIIDQIIVSGALLSGEGKIYTTLDDAHVFNEDFLLETDQKFTGKKVKR
                     TYIGFKYHGGYSDHLPTYVDLKQNKDKVASE"
     gene            35336..36613
                     /locus_tag="PKN53_00950"
     CDS             35336..36613
                     /locus_tag="PKN53_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD87554.1"
                     /translation="MIEIINSIRKYNFWDFNPIDLGFARTFYTNKIGQYLGNKLVKVL
                     VGQRRTGKSYILRQIAGNLLSEGVKSENILYINKEYIELTGLRNAVDLEQLYKTYRET
                     LKPKGKVYIFIDEIQYINEWERFVNSHSQDFAEPCELFISGSNSNLLSGELATLLSGR
                     YIEFEILPYSYIEFCELTQQEIGSKTYKNFLQSGALPELFNLPNDEMKQNYISSIKDT
                     VMLRDIVARYNVKDVKLLDDLFVYLVNTASNLISITNIINFFASKNRKTNYETLSSYI
                     SYLENSFLIHRVERYNIKGKDTISGNSKYYINDLGYRNYLYSGNGYGIGYLLENAVYL
                     SLRRAGYQVYVGTIQNSEVDFVAIKGERKIYLQATLQLTEEKTIEREYRALKLIEDNF
                     DKYVVSMDDYKIPSNEGIEHISAWNLENVLNSL"
     gene            36647..37435
                     /locus_tag="PKN53_00955"
     CDS             36647..37435
                     /locus_tag="PKN53_00955"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOD87555.1"
                     /translation="MKENLKLISTEYLEEYSNKVGVDLFEIFNKLEIKNQFSFEDFEY
                     YIISSSLYSSKIEGNTLDANSFFRSRGKKTSPKKKEVEEIETLMEAYVFASDNKLNRT
                     NFLKSHAILSKTFLPTKERGVLRKEQVGVRDSKTFRPVYLAVEPEFLKVEFDNFFDDI
                     DELLKRDLSHTEIFYFTSMLHIWLAQIHPFTDGNGRAARLLEKWFLVSKLGKSAWSIN
                     SEKYYWDNRTDYYQNISLGYNYYALFWERCISFLLMLPKAVGLS"
     gene            complement(37490..38692)
                     /locus_tag="PKN53_00960"
     CDS             complement(37490..38692)
                     /locus_tag="PKN53_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD87556.1"
                     /translation="MILRQQYIEQLIALKDTNLIKVLTGVRRCGKSTIMQMFKDYLLK
                     NGVSEKQIVFLNFEDLDNEIWLKNYKGLYKEIIGRLDPQKKSYVFLDEIQQVEEFERL
                     IDGLYVKPNIDVYITGSNAYLLSSELGTLLTGRYISIHVLPFSFKEFLYTQPENARTD
                     LMFNKYLVTGGMPGIFELPDFAVQNYIQEVIQNIIQKDILVRYNWRNEDHFNKTTAFL
                     FDSIGSVISPKKITNILRTENAISLSHNTVGNYINALTEAYLFYKVKRYDLQGKALLA
                     TQQKYYSVDLGFKKYYLSDKANLDLGHNLENVVFLELLRRGYRVNIGKAGNTEVDFVV
                     RNPKGNMEYIQVAWTAKEKNTYEREIKSFELIKDFNKRTLITSDVEPITYFNGIKKIN
                     IIDWLLNE"
     gene            complement(38852..39286)
                     /locus_tag="PKN53_00965"
     CDS             complement(38852..39286)
                     /locus_tag="PKN53_00965"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD87557.1"
                     /translation="MAISYRRLEKFKQALKYYQKSMDIYPEGYLAILNTAVIYSFMND
                     YDKSLNYYNFLTYLYADDPEGYFGAGKILFLKSDYKNALENVFIAHIMYVNTNNTDYI
                     KDSEYLISLMYKELKEKGMLDLFNEVAEKFNIKINEDENQNQ"
     gene            complement(39298..39747)
                     /locus_tag="PKN53_00970"
     CDS             complement(39298..39747)
                     /locus_tag="PKN53_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87558.1"
                     /translation="MKHLKVFIIVFLLATCIKAQTFYEKVAIKSCDCFSSLETLQELE
                     DSVVDCVSAAMSRVLNENPADEMEILNTVDGVNVAFEEVGKLLYSYCYSVRRLIINEK
                     ESEFYQLSSNQKANEHYSTGNYFMDKGDYKKSISEFKKAVKIDKILS"
     gene            complement(39902..39988)
                     /locus_tag="PKN53_00975"
     tRNA            complement(39902..39988)
                     /locus_tag="PKN53_00975"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39952..39954),aa:Ser,seq:gct)
     gene            40098..41021
                     /locus_tag="PKN53_00980"
     CDS             40098..41021
                     /locus_tag="PKN53_00980"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOD87559.1"
                     /translation="MKREIVLAYIGLAVMTLIIGFSFIFVKTGLNYSNPYDLLAHRFN
                     MAFLVILILLIFKKIKFPKVSFKKLMKLILIAMFYPVLCFGLQAIGMVNSTASEAGLI
                     FSFLPVITLVAGNIFLKEKSSTVQKIGVLLSVFGIVFILYFKQGLSNFNVQSIIFLFL
                     SVFSMIGYFIFGKKEISKIDSLSLTAIMISLGFVVFNVVSISRHLAINKDLNLYFEAY
                     KSSQFIISVVYLGVLSSVLTSFLSNYSLNYLPTFKVSIFSNLNPIIAIVAGVLILKDD
                     FYWFDFIGVSLVFIGVIMVLFYKPKQKQATV"
     gene            complement(41034..41684)
                     /locus_tag="PKN53_00985"
     CDS             complement(41034..41684)
                     /locus_tag="PKN53_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOD87560.1"
                     /translation="MRYFLLVILFNLAFNFGFSQTYRIALLKYSGGGDWYANPTSLPN
                     LIKFCNSNLGTNINPEPATVEPGSADIFNYPFVHLTGHGNIVFSDYEARNLRNYLIAG
                     GFLHIDDNYGLDQYIRREMKKVFPESEFIELPFSHPIYHRKYDFPKGLPKIHEHDNKP
                     PQGFGIIYQGRLVVFYSFETDLGDGWEDEEVHNNSYETRLKALKMGANLINYVFEN"
     gene            complement(41688..42395)
                     /locus_tag="PKN53_00990"
     CDS             complement(41688..42395)
                     /locus_tag="PKN53_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87561.1"
                     /translation="MKKSILILFALISLTAFSQRNSETVKDKDLYGSDTKWSCADKHF
                     GYFYVNYSKPIPINNSNIEKSLCSNIFSAGYTYRFKIAKPFDIGAEIAFQKRTSSIKK
                     DDLPTFDPSMFYDKISSFHNYASGGIYFRFNLAGATYRNLGYYIDLGGLYSYAFSYGM
                     KYRMDNEFLNQKLKFKKPEYLSPFEYSLFLRVGLSNFALIASWHIDDWISDFKPENLN
                     FKRNPLTVGVQLNLYSK"
     gene            complement(42418..43578)
                     /gene="dnaJ"
                     /locus_tag="PKN53_00995"
     CDS             complement(42418..43578)
                     /gene="dnaJ"
                     /locus_tag="PKN53_00995"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOD87562.1"
                     /translation="MAKRDYYEILDVNKDASAEEIKKAYRKKAIQYHPDKNPNDKEAE
                     EKFKEAAEAYEVLSNPDKKARYDRFGHAGMGGAGGFSGEGMTFDDIFSRFGDIFGDAF
                     GGGFGSFFGGGGGSGSRTRVNRGTNLRVTVKLTLDEIANGTTKKIKVKKYVACKTCGG
                     NGEKNGNSSKTCPTCNGRGVQTRVVNTIMGQMQTSTTCQTCGGDGKVIQEKCTACYGE
                     GVVTGEELIELKIPAGVSEGVQLSVSGKGNAARRGGINGDLIVLIQEIEHPEFQRDGN
                     NLIYNLFISIPDAVTGTNAEIPTLSGKVRIKVEPGTQSGKILRLRGKGLPDLNAYSKG
                     DLLVRINVFIPSSINKEDKALLQKMKESDSFNPKDKKTESIFDKFKGYFSAF"
     gene            complement(43582..44202)
                     /locus_tag="PKN53_01000"
     CDS             complement(43582..44202)
                     /locus_tag="PKN53_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOD87563.1"
                     /translation="MNENINNSQDLENKEAKDIEIENSEDTCDSINNEQNEQVEEKKT
                     YKSSGRRKERKLKEEISLLQQQNDELKDKYLRIVAEYENFRKRTAKEKLEIRETAKAS
                     LMLDFIQVVDDMDRAMQHLDNVKDIESTVEGVKLIHQKFLDFLKAQGIEEIAAQDLEF
                     DLDFHEAVTRVPVEDEEKKGKVVDVVQKGYKLNDRVIRYSKVVVGE"
     gene            complement(44305..44967)
                     /locus_tag="PKN53_01005"
     CDS             complement(44305..44967)
                     /locus_tag="PKN53_01005"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOD87564.1"
                     /translation="MKKFLILILIAHFSNFIFSQQVGINIGDIAPDIKLPNPKGDSIS
                     LYSLRGQVVLIDFWASWCGPCRKENPHVVQAHEKYHDKNFKIGKGFTIYGVSLDKNKE
                     NWEKGIKEDKLNWHNVSDLAYWQSPVAGKYGVKGIPSNFLIDKDGIIVAKNLRGSILE
                     ETLEKYVIIDPVISFEDKLKDLKLEYNNLEHSDEYGNSKELKKLKKNIDNIEKSIKTL
                     KK"
     gene            complement(44979..46289)
                     /gene="murA"
                     /locus_tag="PKN53_01010"
     CDS             complement(44979..46289)
                     /gene="murA"
                     /locus_tag="PKN53_01010"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOD87565.1"
                     /translation="MSTFKVTGGYKLKGDVYPQGAKNEALQVLCAVLLTEEKVKISNI
                     PDIRDVNKLIELLGSLGVEVTKNGKGVYTFQAKDINLEYLQSDEYRADGSALRGSVMV
                     LGPLLARFGSVCLPKPGGDKIGRRRLDTHFLGFQKLGAEFIFDPKDNFYTLSGKELKG
                     TYIHLEEASVTGTANIIMAAVLAKGETTIYNAACEPYIQQLCKLLINMGAKISGVGSN
                     LLRITGVEKLGGAEHKILPDMIEIGSFIGMAAMTSSEITIKNVNYDMLGIIPNSFRKL
                     GIKLERINDDIFIPAQENYEIESFIDGSIMTIADAIWPGLTPDLLSVFLVTATQAKGA
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGLDRAIKLRATTMQSPDIRAGM
                     ALLIAAMSAEGTSIIHNIEQIDRGYENIDQRLNQLGAKIERINY"
     gene            complement(46296..46922)
                     /locus_tag="PKN53_01015"
     CDS             complement(46296..46922)
                     /locus_tag="PKN53_01015"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HOD87566.1"
                     /translation="MDYNTQRPQLKISEYGRTIQQMIDYACTIENREERTKAAHTLVA
                     IMGNMYPQFKDVSDFRHKLWDHLAIMSDFKLDIDWPYPLPDIKLLKEKPEKIPYSEGK
                     IKYRHYGQIIQRILNDVSNIEEAEKKEVLIEQMANQMKRSYMTWKKENISDDIIFKEL
                     EILTKGGVGIPEGLKLNDFREVLVQNKPNNPKQASKASSKKRSSRRKK"
     gene            complement(46976..49294)
                     /locus_tag="PKN53_01020"
     CDS             complement(46976..49294)
                     /locus_tag="PKN53_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOD87567.1"
                     /translation="MENYLLKDLDATQKTAVKYCEGPQLVVAGAGSGKTRVLTYKIAY
                     LISQGIKPHRIMALTFTNKASNEMKDRIDKLLGYQASQNIWMGTFHSIFGRILRIEAN
                     KVGFDSNYTIFDAADSLSLIKTIIKELNLSDDTYKPKAVAARISAAKNELWTAEGYAQ
                     NNDFLIQDYKENREHFHAIYTKYAQRCKASNVMDFDDLLLYTNILFKISPETLAHYCQ
                     RFDYILVDEYQDTNYSQYLIIKKLSSTHRKLCVVGDDAQSIYSFRGAKIQNILNFKND
                     YPDYQLHKLEQNYRSTQNIVNAANSLINKNKNQIQKKLFSENTIGSKIKIEETITDGL
                     EGIAVAETISKLLADNNYTASDCAVLYRTNAQSRIMEEALRKFNIPHKIHGGLSFFQR
                     KEIKDVVAYIRFSVNQKDSEAFRRIVNYPSRKIGATTVNKIFQFSEDSNIPIWEIINY
                     PENYNVGLNVGTQQKLKSFANLIDSFAKQIETSNASEFLVEVVNKSGINHELFSDKTP
                     EGISKYENLQELFNAAQNFCDQRKALAESDSIIDYLENISLLSDLENNEEEDNNENDK
                     VSLMTVHFAKGLEFKNVLIVGAEENLFPSAMSIQSTVELEEERRLFYVAITRAKENLF
                     IFYATNRFKFGNVQSMRPSRFIREINSDFVEWEGKDESQTPSYTNERTGFDEIFESKP
                     QYKTFENKPQKQLSQVPKGNFVSVNKASKTNTGTIDISAYKVGMRVAHNSFGKGEILE
                     IIDEGSNSKLIIKFDTGDTKTLLLKFAKLLIL"
     gene            49662..50135
                     /locus_tag="PKN53_01025"
     CDS             49662..50135
                     /locus_tag="PKN53_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87568.1"
                     /translation="MKTTKIIFLLAFIFGSFICNAQEVNQKLLVKFDANELKEMKKTN
                     VENLNFLEYYVSDALYFVPVPDKPVNYKELVRINPKTKAISENQTITQADIDDFNPLE
                     YDISYEPDRYTYYLAGNTGMLLVVPSQRDLKLGSDNRVKSLKLQQTQQLDKINKK"
     gene            50142..>54678
                     /locus_tag="PKN53_01030"
     CDS             50142..>54678
                     /locus_tag="PKN53_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOD87569.1"
                     /translation="MKKLLSLILSVFVLSLYGYGQYDMVTNNGQTISACSGSLSLGSY
                     TVGQTYTLTICSNDPVNKHITFSVTGGYTFPAGTEVCFYDGASTSAPLILCWDSGTTS
                     GQLAVQTGGGNESGCLTVRFTGGAAGASLGTNNISCNFVCQPREVVIVSSDPPLVGGT
                     YIDVCWDEANNQTFPITFNAQGTYPATGYECTDATNTFTWNFNDGSPLVTGIGMSTVT
                     HTFPERRGYTVTVNIEDSEGCVNTNAEQKRVRVSLPPIWNNSTTVLTTTPPTTPPAIC
                     MGSPVNACAYWSNPQWTSVIALASGDTVCVPDTPPLCYESTLIETSFEPGQILTSIND
                     IISIDMNLAHDYLGDLTFYIICPNNQTVMIGNQGGGSANLGVPGPDRNSCEGQQGWMY
                     HITPTATQTMQQAGSGIWSGSLPSGNYASYQSLTGLVGCPLNGQWKLKICDNWNLDAG
                     TIFGWSIQFQESLYPEVWDYSQTYTPTIWDGLYGSVVNDPNNQNCMTGTYITSATPDV
                     NSLQPFTITITDNFGCTHDTSLYVTVRHQLDEECCQIPNVNAGADVAVCALTYDLSAS
                     PLAYPSNTGNWELISGPGTATFTNVTSPTTTVNVSVYGTYVFRFHERYNDNPGCASSD
                     DVTITFNPTYNPTLSPVANMCVNAPPFMLEMVDFGTLSVNINPELLNVTTGQFNPSKP
                     GNYTITNTVQDPCSGGSLTHSITFTVHDAITVQNFTEYCGPTGSPTYFYVEFDVVGSQ
                     GNPFTGYYVNGTLQNNPHYTATIQSPGSYGYTVTDAYNCSSYPINGFRDCGCPFYAGT
                     MASLQTVILCQTECSGDEVSHNNNQETDGGAGVFEFMIHNGNNQPIAYSNTPNFCRNS
                     TTPPLSFNTLYYISPICGLPTGPGGHANITPPNCYSIGQGTPVMWLQDPVAHVGPSKD
                     TCGLIIQLQGNAPTDGMYGYWSSTCDFITINGTSHTDPNAVVMVSDYEDCTFTWNIVN
                     GECVANADVLMRFLATPAPYAGPDLVVCGNSAEMAGVASMGGNLTWSGSGTIFNPQTS
                     ADATSNISVFGTYQYTLIEDNGTCTGSDKVLVTYIKEPSPITTPNVDTACGVTYNLKV
                     TTVPNAVGQWTSYAWNEATSEWELAYPYMTPNSQSTDVQVQIGNFSGITRRFRFVWTE
                     TNTTGGIVCQGTASKEVVFAKIPQASVVQDQLEVCGSCVDLHANVTSETYNYDYYWIP
                     KIDGNWTNPEGRFSPDASFCVEGLAVFGDSASVNAEFYWIVRNGTSCVSTAAVNVTFH
                     QQPQANAGLDTIICGLEANLHAYWSFPPNPSYSPSGVWSTVPKPGATATIQDKYNPIS
                     PVTVSEAGVWNFVFRENNENLGSCYSTDTVQIEFVETPIPFAGEDKHVCGKCVTLEAT
                     SGGFSGTWLPNGASFVDYEDPHTNACVAAYDTVVFIWQESNPSITDPAFACVATDEVN
                     IIFWRVPTANIITAVADSAVCGLTFYELRAELPGTGITGYWYCHNSGLEYGDKFSNNT
                     WVKVPHYGYYDFYWIEQT"
BASE COUNT        18580 a         8207 c         8891 g        19000 t
ORIGIN      
        1 gattacaggc aaaggatata aaatagttga tagacaggat ttaattacat ttttttcaaa
       61 taatgtggtt gataatgaca tttatgttga ttttgtggag aatattaatt cgcttgaaag
      121 ttcgataaaa tcatttgaaa ctttacctat aaaaaactgg gattattata gttggcaagg
      181 attttatcaa tttttagata gccaaattaa agtagtagat tggcaatatg tttctaatcc
      241 tgctggaggt tttttgggtt tatggtggca ttttatagac tgggaaggtt ataaggtgta
      301 tttacaaata gagcaagata atttttgctt taaaattgga ccagtagaag aaaaacgtag
      361 cgctgttaga aataaatggt attcagtatt atcaaaatac atagagtcta aaaaaatgta
      421 tgaaatcaaa aaaccaacac gatttggaaa tggaacatat atgactgtag gagtggtttc
      481 tcgagaggat tggcttggga aagacaacgc tatattagat aaggatgttg taatacgaaa
      541 tttgaaaaaa tatgaaaaat ttctatcaaa ttgcgtaaga aactcaaaga gaaacgttaa
      601 ataagaatat gtgttagtgt ttgatagtca gtttgtttaa gatgttgaag ctgattttaa
      661 aagtaatttt agtctggtag aaaacttata aaaactcaag agtttatata tgtaaactat
      721 tgtgaaaaag tttaaatatt cacaaaataa attttataaa ttaccttccc tcttttcttc
      781 ccacaaaaaa tttctaaaac tcattatttt ttttattttt gcagaaatag taaaaaccta
      841 aaaattttaa taaatatgaa acaaattatc gaatgtgttc caaattttag tgaaggtcgt
      901 gatatgtcaa ttataaagca aattacagat gtaattactg ctattgacgg tgttaaactt
      961 ttagatgttg acccaggtgc tgcaacaaat cgcactgttg taacttttgt aggtacgcca
     1021 aacgaagttt tagaggctgc ttttcaagct gtgaaaaaag cttctcaaat tattgatatg
     1081 agcaaacaca aaggagccca cccacgcttt ggagcaactg atgtttgtcc acttattcct
     1141 atttctaata taagcatgga agaaactgct gaactggcac gaaaactagc taaaagaata
     1201 ggcgaagaac taaatattcc tgtttattgc tacgaaaatg ctgcatttac agaaaaacgc
     1261 agaaatttag ctaactgccg tagcggtgaa tacgaaggtt tggcgaaaaa aattactagc
     1321 caagagtgga aaccagattt cggctctaat gtttgggacg ctcacacaat gagaacaggt
     1381 gctactgcgg ttggtgcaag aaatttctta gtagcatata atataaacct aaacacaact
     1441 tctgtgttta aagcaaactc tgtagctttc gacgttagag aaaaaggaag agtaaaaaga
     1501 gaaggacatc cagttacagg aaaaccagta cttgataaag acggaaataa agtttacgaa
     1561 ccaggacttt taaaatcagt aaaagctatt ggctggtata tcgaagaata tggagtagct
     1621 caaatttcta tgaaccttac agatattagc gttacacctt tgcatgtagc tttcgatact
     1681 gtttgtgaaa gagcaacagc taatggttta agagttacag gctcagaact tgttggtgtt
     1741 atgccattaa aagcaatttt agatgcggga aaatatttct taaaaaaaca aggacgttca
     1801 acaggaattt cagatgctga aattattaaa attgctgtaa aatctttagg attagacgag
     1861 ctaaaacctt tcgacccaaa gaaaaatatc atagagtata tgcttgaaga tgacaataac
     1921 aaaaaacttg ttgacatgaa tttagttgat ttctgtcatc ttgcaggctc agaagcacca
     1981 gcaccaggtg gaggttcggt atctgcttat attggagctt tgggagctgc tttgggaaca
     2041 atggtagcaa atcttagtgc taacaaatat cgccacgacg accaatggga aaactttagc
     2101 gattgggcag ataaaggaaa agcttatcat gacgagttac taaaacttgt tgacgaagat
     2161 actgttgctt attctagaat tattgaatct tacggcttgc caaaaggaaa tgatgaggaa
     2221 aaagcactac gctcacaagc aattcaagat gctacaaaat atgcaattga agtacctttt
     2281 aaaattatga aagtttgtta caatagcatg gaagttatgt tagaaatggc aaaaacagga
     2341 cttgaagctt cggtaagcga tgccgctatt ggtgctattg ctgcaagaac aggagtacaa
     2401 ggcggattcc taaatgttaa aataaatgca cctagctata aagacaaatc ctttgttgat
     2461 aaagtaattg cagaaggtaa tgaaattgaa agaaaagcca tagcccttga aaaggaaatt
     2521 atggagattg cattagccaa aatcaacaaa taatgcggat taaattaaca attggcatat
     2581 tgtagaacct acgcatgcgt aggttctaca acaatgccaa tttatttata ttccaaaata
     2641 atttttaaaa aacactcaaa aaaattgttt ttaattctgt ttttatgtat ttttgcataa
     2701 aattagaagt aaaacaggca tggatttttg gcaattaaga aatcaatttt ataacttggt
     2761 ttgttttaat atcaatcaag tatatgcttg gcgacctaac tttgagaaaa ctaacctcac
     2821 tcgttgggtt aagcagaatt atattataaa actacgtaat tcttggtata gttttcctga
     2881 atatttagaa actcctaata ttcagcactt tatatctaac aaaatttata gtccatcata
     2941 tataagttta cactctgcat tagcgtttta tggcattatt ccagaggcaa tagtaagaac
     3001 aacagcagta agttcattga aaaaaatgag ttttgagaat gcttttggtt cgttttctta
     3061 tcagcaaatt ttgccagaat taatgttcgg tagtgaacaa aaaatatttt taaataaaca
     3121 tagtttgatt tttgcttctc ccgaaaaagc aattttagat ttactatatc tttatccaca
     3181 atatactaca gaacaagaaa tcagagactt gcgttttgac gaagaattta tgcaagagga
     3241 tttaaactta gaacgtctta atgagtttac agaaagattt cagtcaaaaa tattacgaaa
     3301 tagagttaat acacttttaa aaatttatga tttatgctaa attttgaaca aataaaaaat
     3361 tattttcctc cacaaataag agaaaatcct gcacacaata aatatattct aaaggagtat
     3421 attcaactga tgattttaaa ttttttatca agctcaaaat atgtaaaaaa aattgctttt
     3481 ataggtgata caaatttacg tttgttaaaa ggaattgata gattttctga agatttagat
     3541 tttgattgta aagatttttc tatagaagaa tttgttgaaa tgtcgcaatc ggttttgact
     3601 tttttaaaac gttctgggat aaaagccgaa acaagagaaa agcaaagtga taaattaact
     3661 gcttttcgtt caagatttta ttttccagag ttattgtttg aattaggttt gtcggcatac
     3721 aaggaagaac gatttttgat taaaatcgaa agtcaaaatc aaaaatttga atatacacct
     3781 caaatggcaa cgattagcgg gtgcgggttt tttatgtcgt ttccagttcc acctgatgat
     3841 atactttgtg caatgaaaat atctgcatta ctttcacgag caaagggacg agatttttac
     3901 gatgttatgt ttttgctatc tcaaacaaat ccaaattatg attatcttgc tcaaaaacaa
     3961 ggtcttcata atttaatgga actaaaaatt aaattggaag aaactataaa caaagttgat
     4021 ttacataata aatctaaaga ttttgaacat ctgcttttca ataaagaaaa caaaaataga
     4081 atactgcatt ttaaagaatt tgtagaaaaa ttgaattagg aaaaaatccc aaaatcgaaa
     4141 tataaaaagg cattaattaa taaatggcat tgttgtagaa acaacgcatg cgttgtttct
     4201 acaacaatgc caatttattt atatttcaaa ataattttta aaacagcatc ttatttcgtt
     4261 gcaacatcaa ttcgaacaaa cgaacaaatg tcgattagga caacatagac cctcaaaggc
     4321 ttgcccgtag ggcaacatgt gattaaccgt aggcgaagcc tgcggtgaga atcccctcaa
     4381 aagctttgcc cgcaagggca acacaataaa cagcccttat ttcgttgcta catcaatagt
     4441 atttacaatc gcagttttat atttttctgg aaaattagga attttattat tatcggtatc
     4501 ggggaaggat tgtaaaaatt tcacaagagc tatccattct ttaccttctt gaataccagg
     4561 tttgttttcg tcaaaatcaa tccaagtggt tttattattt gtgatttttt cgccttgtgc
     4621 atttttagga acgattttta ccaaacccat tgtcataccg cgaactttac caacaaattc
     4681 taacatatat gagtttgctg ccagactata aagattttta ttttttttcg atatatcaat
     4741 ttccttaccg tctaattcaa ttttaacaac ttttttcagc actccttttt cgctattata
     4801 ataaactttt gcaccgctaa aataacagag gtaagaagga ctcatttttg gagctaacaa
     4861 taaaacctcg aacaagtttt taatttcttt agcagttaaa taaactttag ctaatggata
     4921 gccaggcatt gagtcgtcgc cttcgcctaa gctcataatt ctaaaaacat caacagcagt
     4981 ttgaatacca tttttgccaa cacgaaattt atcacgaata accccagcag caactaaaga
     5041 aatgtcagct ttttgatttg aaaaattatg tatataataa tgaatagcat cagcaacaag
     5101 tggtcccaaa ttgcttttgc ttaattcttc ttctctgtcg caacccaatt ggtagtcggt
     5161 ttctgctaaa ggtctaaaat aaccaagacc tagaggacgt aaaatagagt cgtcaattaa
     5221 tttaattcta tttgaaatta tttgatgaat tttacaatca ccataaattt tgtcgtcaac
     5281 agctaaaagc tgaaatcttg ctgattttac ttttccactt tctacattca tttcaaatct
     5341 acctaaattt tgaccttggg caccagtttg cacaatataa gtattgttta caataattgg
     5401 ctcatgaatt tctgtatgag tatgtccaga aactattaaa tctattcctt ttacttttag
     5461 tgctaattca acatcttcgc cagtccagtt tccattttta tctttagtaa caccgcaatg
     5521 tgataaacaa ataaccacat caactttttc ttggtagcgt aatgttttta ctatttgttt
     5581 tgagattttt attctatttt ggattataac aggttttgca tttggtgcta cttcgttagc
     5641 atcatcgccc ataattccaa aaaccccaat ttttaaacca gctttttcaa caattgtgta
     5701 aggttttata attccttttt caaataattt ttcaagatta tcatctaaat ccgatttttt
     5761 tgacaattct gcattagaaa gtacaagttg tggaatttcg cctttttcta aatttttatt
     5821 aacgatttgt gcaaaagcat taggtccaaa atcaaactca tgatttccaa ttgatacaac
     5881 gtcatagccc atttttttca ttaaatgcaa ctgaaagccg tcattcactt caaaaatatg
     5941 gaaaaaagta cccattaaaa agtcgccagc atcaaccact aaaacattat cggggtttgc
     6001 ttttttctca gcatcaatca aagctgcaat tcttgcaaaa ccaccacgag tatcatcgtc
     6061 atcaacagaa caaggagtat attcactttc tggagaaaat cctgttaaat tgctgtgtaa
     6121 atcattggag tgtaaaacaa taagctttcc agagttggtt tgagaaaatg caacaacgta
     6181 caaaaacaag gaaataaata aacaaactaa tctcataata ttaaaatttt acaaaaaata
     6241 attattaaaa tttaacacaa atataggttt tttaatttat aaacttgtat ttattctaaa
     6301 aaatataaag ttctataaat cttttattgc atagctttgg cttgtcgtaa cagtaattta
     6361 acatctaatt tttgtaattt tttaatttaa aaaacaaagt ttaatcaaaa tcaataataa
     6421 attagtttat aaaattataa atttgcatat atttgaaaaa ataaatatgg aagataaaaa
     6481 aaatcaagat attttagttt atacagattt tactgaagtc ggcgaaaaaa gcgttttgtg
     6541 ggcaagtttt ttagctagaa aatttaaacg taatttgcag attatacatg ttataaacga
     6601 aaattcttta atatatttta gcaaagatga tacttatgat gaggcaaagg aggctttggc
     6661 agcactttgt gaagatataa gttcaactca cggcataaaa tgtaaatata ttgttgaaga
     6721 aggctgtact tgtactatta ttaattcaaa tgcagaaaga atagatgctt tttttgttgt
     6781 tttaggaact catggtaaga acgacccaca atttttatca ggttcttcgg ctataaaaat
     6841 tattagaaaa gcaagaattc catattttgt tgttcaaaaa aactcgccac taccagacag
     6901 taaaaaacga tttgtcttgc ctatgaactt taaaaaagaa gcaaaacaaa aagttggttg
     6961 gactacatat tttgctttaa atctaaaatt agaaatagat ttgttttatt atgaaaatcc
     7021 acaagataat ttaaagaaaa atattcattt tactaataca ttttttaata attactcttt
     7081 taaatacgag cataaacctc agccaaaaaa atataaaagt atagaaaaac tagcagtgga
     7141 ttatgctgat gaaatagatg ctttttgtat ttgtctttta atttcttcta aggagaattt
     7201 agttacaaaa atttttggtt ttcccgaaac atcaataatt acaaactctg ccggaattcc
     7261 agttttgtgt gtgaatccta aaaaagactt gtatattccg tgtatttaaa ggcgtaattt
     7321 agaatgtttc taaacatatt tatttttaaa acataatgct taaaaatttg tatattaata
     7381 aaatagaaaa aataaattta ataaaaacac tttaaaacta aataaaaatg ataaaattaa
     7441 aacaactacc ttacgaaatg aacgcactag aaccatatat aagtgcaaag actgttgagt
     7501 ttcattacac aaaacaccat caaggttatg tgaataattt gaataattta attgaaggca
     7561 ctgagtttgc cggcaaagat ttagaagaaa ttataaaaac ttctgaaggt ccaatattta
     7621 ataatgctgc tcaagtttgg aatcatactt tttactggga aggcttttcg gctgatccac
     7681 aaaaagaacc aaaaggaaaa cttgcagaat taataaatgc taaatggggc tcttttgaga
     7741 aatttaaaga agaatttaca aaaaccgcaa caggtttatt tggctcggca tgggcttggt
     7801 tagttattga cccaaaaggt gaactaaaaa ttgtaggaac ttcaaatgct gctacaccgc
     7861 taaccacaaa cgataaagcc ttgctaacag cagacgtttg ggaacatgct tattatctag
     7921 attatcaaaa tcttagagca acttatttaa aaagtttttg ggaattagta gattggaaaa
     7981 aagttgaagc aagaatgtaa aagtttgtgg cgggcaatta ttttgacccg ctcagctttt
     8041 taatttcatt taatttattt agagcttcta agggagtaag actgtcaatg tcaattcctt
     8101 gaagctcttt ctttatttgg gaaagtactg ggtcgtctaa ttggaaaaaa cttagctgat
     8161 aaccttcgcg actagtagca attttttcta caggcttgtt taaatttaca cctttgttca
     8221 tttctttaga tttgccttca agttgttcta agattttttt tgctctatat accacagact
     8281 ttggcatacc agccatttga gcaacatgaa ttccaaaact atgttcactg ccaccttctt
     8341 taagttttct taaaaagatt accttatctt ttgattcttg aactgaaacg ttgaaatttt
     8401 taatacgctt aaaagatttt tccatctcgt ttagttcgtg atagtgagta gcaaataaag
     8461 tttttgcttt tgctgtagga tgttcgtgaa tatattctgc aatagcccaa gcaatgctta
     8521 tcccatcata agttgatgtg cctctaccaa gttcgtctaa aagtattaaa ctttttggag
     8581 ataaattatt cataatgctt gcagcttcga gcatttcgac cataaaagtt gattcgccca
     8641 tagaaatatt atcagaagca ccaactcgag taaaaatctt atcaacaatt ccaatttcgg
     8701 ctgaacttgc aggcacgtaa gaaccaattt gtgccataat gcaaatcaaa gctgtttgac
     8761 gcaaaagtgc agatttacca gacatgttag gaccggtaat catcattatt tgagtagttt
     8821 tgtcgtcgag ataaacatcg tttggaacat attcttcacc aaaatcaagc attttttcaa
     8881 taactggatg gcgtccatct tttatatcta aaacattgga ttcgttgagc tttggtttgc
     8941 aataattatt ttgaattgcg gtaacggcgt aagaatgtaa acaatcaatt tctgcaatta
     9001 tgccagcagt tttttgaatt aaaccaacat attcgccagc aaaaagaact aaatcattaa
     9061 aaatttggtt ttctatggac tgaattcttt cttcagcacc cataattttt tcttcgtaaa
     9121 gttttaattc ttcattaata tatctttctg ccgaaactaa agtttgtttt cttacccatt
     9181 cttcaggaac tttatcttta tgggtatttc tgacttcgaa aaagtaaccg aagacattgt
     9241 taaaaccaat ttttaaagaa gtaatacctg ttcgttcggc ttcgcttttt tctaaatttt
     9301 ctaaatattc tttaccaccc gatgctaagg atcttaactc atctaattcg tttgaaacac
     9361 cttgagcaat aacatttcca cgatttagca aagcaggaca ttcgggatta atttcaagag
     9421 agattttttt cactaattca tcgcatagtt caatgttttc gcctaaattt tgaattattt
     9481 tgttgtcaga atttttaaac atttctgcta ttggttgtat agcgactaat gaatttcgta
     9541 gcgacagcaa ttctcttgga ttaactctta aaaccgcaat ctttgaggat aatctttcaa
     9601 tatcagaaat ttggtgtaat tgttctttta attttgtgaa aaaatcagga ttatttataa
     9661 aaaattctac aacatcaagt cttctattga tagcatctaa attttttaaa ggtaagctaa
     9721 gccatctttt tattaaacgt ccacccattg gacttagagt gttgtcaaga atttcgatta
     9781 ggcttttacc ttcttcgtta ctacttccaa aaagctctaa gtttctaatg gtaaacttat
     9841 ccaaccaaac gtaattgctt tcgtcaattc gcgaaatact tgcaatatgt tcgcgtgctg
     9901 tgtgatgtgt aaaatcgaga tattcgataa tagcacctgc ggcaattatg gcagattcta
     9961 aatttgaaat tccaaaacct tttaaatttg cagttccaaa ttgtttattt agtttttctt
    10021 tagcgtgttc aatggtaaaa acccagtcat caaaaccaaa gtctttgaaa tttcctgaaa
    10081 aattttcttc gaattttttc ttcttatttt tttcataaat tacttcttta ggtttaaaac
    10141 tttgaagcaa tttatcaata tagtttgctt gtccttgagc tacaaaaaat tctccagtag
    10201 aaatatcaag aaaagcaatg cctataaatt gttttccgat ataaattgaa gctaaaaaat
    10261 tattttcttt tctttctaaa acattgtcat ttagtgaaag tccgggagtt actagttctg
    10321 taattccgcg tttgacaatt gtttttgcca tttttgggtc ttctagctgt tcgcatattg
    10381 caactctttg tccggctctt actaattttg gtaaataatt atccaaagca tgatgtggaa
    10441 aacctgctaa atcaatatga ctagctgtgc cattagctct ttttgttaaa gtaatatcga
    10501 gaatagaact tgttttaatg gcatcatctt caaaagtttc ataaaaatcg cctaccctaa
    10561 atagtaaaat agcatcaggg tgttttgctt ttatgctgta atattgcttc atcaaaggag
    10621 tttcggcact cttcttcata aaataatctt caaaaataat tgcgtaaaga tacaaaatta
    10681 aaatgtcaat tttacagttc ttaatacttt gttgaaaact taattaatga taaagagttt
    10741 ttagttgtga gtgtttagtt tttagtagtg agttttttaa acttcacaat gatagaattc
    10801 ctttagtgca agatttgtat atacttgctt cgcagagttc gcaagctcac tcttattagt
    10861 ttttagtgtt gagtttttag taaaatttgt aaaaagttaa aacaattcag ttaaaagtta
    10921 aaagtggttt tttgcttggc attcctttat gatcttttta ctttataaac ttttaactga
    10981 atacgcgtca gccactttta actttacctt cggtgagctc gcaagctcgg ttcaactttt
    11041 aacacttcaa ctacgttcag tgcatcgctt ttaactttta actgagtgta gcagtttgac
    11101 atttatgaaa caccgtaatt caaaacaaca atttttagtg tgcaataaaa aaaagagaat
    11161 taatatttta tatattcgat ttaaaaatat aactttgcac actttaaatt tgaaaaaaaa
    11221 tattttagta agaattattt tattattatg gcaaagagaa ataaaaaagc tgcagaaaaa
    11281 gatggtttta aatcagtaga atctgcattt agtcgtgcag aaaattttat tcaaactaat
    11341 caaaaagcat ttatatatgg cttagcagga gtgttggttg ttgttttagg tataattgga
    11401 tactttaagc taatacgcga acctcatgta aaagaagctt actctgtttc gtttatggca
    11461 gaaagatatt ttgtacaaga ttcttttaac ttggctttaa acggcgatgg cgacaatctt
    11521 ggatttattg atattgttga cgaatacaat aatactccta tgggaaatgc tgctaaatat
    11581 tatgcaggag tttgttttat gagattaaag gattttgaat ctgctataga atatttagaa
    11641 aaattcaaat caaaagatcc tatggttgga gcattagcaa aaagccttat cggagatgca
    11701 aatttagaat tagggaatat tgatgcagct ctagataatt atctaaaagc tattgaacag
    11761 gctgataatg agttgttatc gcctatgctt ttaatgaaag caggaagaac ttatgagttg
    11821 caaaaagatt atgcaaaagc tcttgaaatt tataaaagaa tagatacaga atattacgga
    11881 acatcagagc atagaaatat tgaaaaatat attaaaagat gcgaaatgaa cttgtaaaaa
    11941 gttcaaaact ttttgctctc tttattgctt aacactctat ttttataaaa taaaaaaatt
    12001 atggctagtt cactaaaaaa tttatctcaa tattcagaac aaggaattgc aaaaaacact
    12061 aagaaaaatc ctttgaaaat tggtattgca gtttctgaat ggaatcaaga agttactgct
    12121 tcgctagccg aaggagcaat agaaacacta aaaaaatacg gattttcgga taaaaatata
    12181 gtacttaaat ctgccccagg aagttttgaa ttacctttgc ttgcaagata ttttatcgaa
    12241 catgctaata tagatgctgt aatatgttta ggctgtgtaa ttcaaggcga aactcgacat
    12301 tttgattatg tatgttcggg agtaacacaa ggaattatgc aagtgaattt ggaatataat
    12361 atcccagttg cttttggagt attaactaca gatactctgc aacaagcttt agatagagct
    12421 ggaggaaaac atggaaataa aggtgttgaa gctgctattg ctgctctaaa aatgttgcaa
    12481 ataaaattag attttgaaga agaagatgaa ttaaggtttc aagaaatgaa cagatccttt
    12541 tttgaataag aaaatcaaaa tattaatttc aatattaaac attgttttaa tatttacttt
    12601 gttttcgtgt tcattttcaa aagaatggct agaagctgaa gtaacttata caaaccaaga
    12661 aacagaaagt cttttagtta aacctaatcc tttttcttac tatactttta taagaagcaa
    12721 aaaaactatt gatgaaaaaa caaaagtttt aattcctgaa aatgtttata aaattaaagg
    12781 aaaagatttt gcttatgttg caatgttttt cgatgaggct aactatggaa tggagtcttg
    12841 gagttttgga aaagttttag caggcgataa tattatgcta gttgaaacaa aatatcaagc
    12901 taaaacttgt gcttgcaata cttcgggtaa gttttttaaa gattatttct tagtttatgg
    12961 aaattatttt ttaaaaataa aaacttataa aaaaactaat atatgcgaca agcaagaaat
    13021 ctattctttt ttagacacat actacaaaaa tagttacaat aaggaattta ataatatcga
    13081 ggatttaact aagttgataa gagatattaa caattaattt ttttaaacca cacaacatca
    13141 acaagataga ttttgtgatt tttaaaattg ttaataagtt aaaaaaaact aagttgtaaa
    13201 tcttcgtaat tatcttaatt tgtagtattt ttgagctttt caaattaatt gattaaatgg
    13261 gaaaaattat tgttttagcc aaccaaaagg gtggggtagg aaaaactact acagctataa
    13321 atttagctgc aagtttagcg gtgttagaat ataaagtttt ggttgtagat gctgaccctc
    13381 aggcaaatgc aacatcgggc ttagggtttg atgttagaca tataaaaact ggtttgtacg
    13441 aaaccttaat aaaagatgaa gaccctcgtg gcttaatttt atcttgtaat atccctggac
    13501 tatttttgct tcccagtcat atcgacttag ttggtgcaga aatagaaata ttggatttac
    13561 ctaatagaga aaaaattcta aagcatgttt tagaaaaagt taaagacgat tacgacttta
    13621 ttttgataga ctgttcgcct tctttaggac ttattgttgt aaatgcactt acggcagcag
    13681 attctgtgct tattcctgtg caatgcgaat actttgcttt agaaggactt agcaaactgc
    13741 taaatactat tagaataatt caagaacatt tgaatccaga tttagatatt gaaggttttg
    13801 taatgactat gtatgattct cgcttaagat tatctgacca aatttatgaa gaagttagaa
    13861 aacattttga tactatggta tttgaaacag taatacatag aaatgttaaa cttggcgaag
    13921 ctcctagttt tggacaaaca atttttgatt atgatgtaac gtctaaagga gcaacaaatt
    13981 atctaaatct tgctagagaa ttgctacaaa gaaacaatat gacaaaaatt aaaaacgaag
    14041 aaaaaattct tgaataatat ggcaaagaaa aatgctttag gacgtggttt aaacgcttta
    14101 attgaaagtg gcgattatga aaatttgaat aaaacacgtg aacctaacga aataaaaata
    14161 gatttaatag aagcaaatcc ttttcaacct cgcagtaatt ttgatgaaga agcattgcaa
    14221 gaacttgcag actctatcaa agaacttgga gttattcaac caattactgt tagagcttta
    14281 gaaaatggaa aataccaact tatttctgga gaaaggcgtt taagagcttc taaacttgtt
    14341 ggattagaaa aaattccagc atatatacga aataccgatg accaaggaat gttagaaatg
    14401 gcattggtag aaaatataca acgcgaagac ttaaacgcta ttgaagttgc aatttcttat
    14461 caaaggttaa tggacgaatg taatttaact caagatatgc taagccaaag agttggtaaa
    14521 aaacgctcta cagttgctaa ttatacaaga cttttaaatt taccagcaaa aattcagctt
    14581 ggaataaaag aagataaaat tagcatggga cacgcaaggg ctttgttggc agttaaagat
    14641 actgacacgc aactaatggt ttatgaccaa atcttaaaat atgatttttc tgttagaaaa
    14701 gtagaagaga ttgttaaaga actttcaaac gaagaaaaag taaaagaacc agcaaaacca
    14761 aaaaagaccc caaaaacaac agaagattat ttgcaactac aaaaacattt gtcatcttgt
    14821 tttgcagcag acgtagagtt taaacgttct gcaaaaggta gtggcaaaat tgttatccct
    14881 tttaaaaatg attatgattt agaaagaatc attgctttac ttgacaaatt aaatgtatag
    14941 gttttgaatt gtaaaactct catatatata atttttatat tattcttttt ccctaattta
    15001 atatttgcac aaaatattga agcaaataaa tcaaaaccta aacattcgcc tcgaaaagca
    15061 agtataatgt cggcttgttt gccaggatta gggcaggcat acaataaaaa atattggaaa
    15121 atccctatag tttatgcagg aataggtggt tttggatatt tggcaattaa caatcaaatt
    15181 caatttaata aatataaaaa cgcctacaag cttttagaaa acggtcaagc taatgattat
    15241 ccagatttta atcaagaagc attagtgcat atgatggatg gatatagaaa aaaaagagat
    15301 ttgtgcattt tgggaacagt agcgttttat gtgcttcaaa ttgttgacgc aagtgttgat
    15361 gcaaacctct ttgactttga tgttgacgat aatttaagtg tgagtataaa acctcaactt
    15421 caatcagatt ttactaattc tacaataata ccaggtctgt cttgttctat aaatataaag
    15481 taatgaagaa agtactgtca atattaataa ttctgttaag ccaggcaagt tttgcacaac
    15541 aagacgaaga cactgttttt cagcatagaa aaaatgatgg ttattctatg gcagaccatt
    15601 tagatagctt acttgcttcg tggtattatt acacaggacg agatactatt gcttatttat
    15661 ccttaacaga aagacatact catcactatt tagacattcc agattcaatt tttgaaaaaa
    15721 gaattagaaa aattgttaca ccaattgaat taacttacaa taaaaatgtt caaaaatatt
    15781 tggataaata tgtaaaaaaa ggaaaatgga tagcaccaaa atttcttgga ctttccttgc
    15841 aatatttttc tctttttgaa gaaaaacttg atgcttatga tattccgctt gagttaaaat
    15901 atttaccagt tattgaatct gcattaaatc caaaagcaaa gtcgcgtgct ggtgcggttg
    15961 gactttggca gtttatgtac aaaacgggtc ttggaatggg tttagaaata aattcttatg
    16021 ttgacgaaag aatggatcca gaaaaatcaa cagacgcagc gtgtagatat ttaaaaagtt
    16081 tgcatgcaat gtatgataat tggactttag ctcttgctgc ttataatgca ggaccaggaa
    16141 caattaataa tgcaattaga cgttcaggcg gtaaaacaaa ttattgggaa ctttatccat
    16201 atctacctaa cgaaactaga aattatgttc caggatttac agcaatagtt tatttattta
    16261 cttatgccga taaacatgga tataagccag aatatataga atttatagaa gattttgata
    16321 ctgtaatggt aagaaaagaa ttgcattttg ctcaactaga ttctgtttta ggtatttctg
    16381 ttggcgagtc aagagaatta aatcctcaat ataaattaga tattgttcct gcaaaaaata
    16441 aggaatatcc attaaaaatt cgacgaaaat atttaagtaa atttatggaa ttagaagatt
    16501 ccatatataa ttttatggat actcttttct ttaatcctaa aacaccagtt gttatttcag
    16561 ataaatatgt tgaaactatc gtgccagcaa ctcaaccaga aggaacagta gctttgcaat
    16621 atacagtaaa atcaggagat gctgtaggtt taatatctaa ttggtacggt ataagcataa
    16681 atcagctaaa agcatggaat ggacttgcaa gcaacaatat tagagttggg caagttttaa
    16741 aaatttatgt gccagcagct ttagagtcga aatataaaat tgtgaatagc ttgacttttg
    16801 aagaaaaaca aagaatggca ggagtaaaca cttcgagtac tccagtaaaa gaagaaccta
    16861 tcgacccagc atgggaatat tatactgtaa aatctggcga cactccttat ggaatatctt
    16921 taaaatatcc gggagttagt gttgatgata ttatgaaaaa taataatatt acaaatgctt
    16981 ctggcttgag aataggacaa aaattaaaaa ttagaaaaaa ataaaacaat caaaatatga
    17041 gaaatatagc agttttagct ggaggatata cttccgaaag acacataagt ttaaattctg
    17101 gaaagcaggt tttagaaagt ctcgacaaaa ctttgtataa tgcttttttg gtagaagtta
    17161 atgttgacgg attattttgt attgacaaag aaaaaaaata tcaggtcaac ttaaatgatt
    17221 tctcatgtgc attgcccgaa aaaactgtaa aatttgattt tgcatatatt gtccttcatg
    17281 gttcgccggg cgaagatgga aaaattcaag gttatttaga tattttaaat attccatatt
    17341 cttcttgttc ggtttatgcg tctgcaataa cttttaataa gttggcaagt aaaaaacttt
    17401 tagaaaacac aggcgttcat ttggcaaaat caatgcgaat agaacagggt atgagttttt
    17461 gcttagacga acttgttgat gaaattggct taccatgttt tgtaaaacca aatactgctg
    17521 gctctagcta tggtataaca aaagtttatg aaaaagacaa actcatagat gcaataaacc
    17581 atgctgcaga agaagatagc actattattg ttgaagaatt tatcaaaggt gttgaagttt
    17641 cttgtggtgt ttttaaaaca aaaaatcaag aaatagtatt tccagttact gaaatttcaa
    17701 caaaaaacga tttttttgat actgaagcta aatatcaacc aggattaaca gacgaaatca
    17761 caccagcacg cattagcgat gaagaaacta aagctgtgca agactttaca tcactaattt
    17821 acgacatctt agagttaagc ggaattgtta gaattgatta tattgttaaa gacaaaaaac
    17881 catattttct tgaagtaaat tcaattcctg gtatgtcggc agagagcatt attccaaaac
    17941 aaattagagc tataaataaa actgttacag aaattttaag tttagtaata gaaagtaagt
    18001 ttgaggatta attataaata tccatcatga taccgcataa ctaaaatttc aatatcgtaa
    18061 tctttaccgg cggcatcgta ttcgcgttta aggttattcc caaacagtac agcaaaagtt
    18121 tgccaaaaaa atgcattaac attacccttt agttctttaa taatatcata agcagtactt
    18181 ccactttctc tatttagtaa tttaaccatt aaaactcctt gtgaaagtgt aaatcgagag
    18241 aggatgggtc tataatattt cattaattgt ttttctcttc ctttcacata aacctctcta
    18301 tatttatcgt taggaaaatt ttttaaagaa tcttcaatat tgttgtaagt tgcagacaat
    18361 tctagaacat atggataaac ttttttgaaa ttattaacca atttgtcgta tcttcttctt
    18421 tctctggcat ttttaaaagc tttttgagga taaatattaa cttgtggaaa cacatataaa
    18481 gggtcagaga tttgttttgc aatactgtca acattaagtt ctttatgata atacttaggc
    18541 tttcctttat cttgagcatt tgcttcaaga aaacttattc ctagaaatat aagaaataaa
    18601 gctataaatt ttattttcat ttcaataatt tttaatgttc attttataaa tattcaatta
    18661 tcataccaaa tatttttata gaacaaaatt tctttaaaaa tgatttttca aataaaaaac
    18721 aaacttattg attattttat tgttttaaaa aataaaaact attaaatttg ccttaaaatc
    18781 tttatgtatg aaaaacgaaa ttaaatccat agaagtatta tttaattcgc ccgattcagt
    18841 gttctcaaaa ttggtaaaag cagataagga atatttgctt gcaaacactg aatatcgtgt
    18901 gtataaaaaa aatgaaatag tattttcaga aaatacaaaa cctacaggtt tgttatgtct
    18961 ttcagaagga aaggtaaaga tatataaaga aggtgctggc ggtagagacc aaattatacg
    19021 cctttcgcgt ccaggtggat ttattggata tagagcttta tttgctgagc aaaattattc
    19081 cgcttctgct gctgcaatag aaaattcagt agcttttata attgagaaaa aagcattatt
    19141 ttctattatg aaaagagatc caaatttaac tttcggaatt ttaaaatctc ttgcaacaga
    19201 acttggagta tctaatatga gaacagtatc tttaactcaa aaacatatta gaggaagatt
    19261 ggcagaatct ttattgtttt taatagatac ttatggatat tacgatgacg gaacaacaat
    19321 aaaagctcta ctttcaagag aagacttggc aaaccttagc aatatgacta caagcaatgc
    19381 aataagaaca ctttcttcat ttgctcaaga aaaagttatt gctttagacg gaagaaaaat
    19441 taaggtttta gaccttcaag agttagagaa aatatcagat atgggctaaa aaataattaa
    19501 agcctaaaac taaaattaat aataaaaaac attatttttt gagtgatata tcccgataat
    19561 ttcgaacaaa aaatcggttt cgataaaata aagcaaatga tttcgcaaga atgcaaaggc
    19621 gaagctgcta aattattggt cgaagactta aagtttagta ataattttga atatattaac
    19681 actttattga acgaaaccga cgaaatgatg agtatttgta tgctaaagga taattatcct
    19741 gcacaaaatt atccagagat aggtattgct attaataaat taaaaattga aggcacaagt
    19801 ttgcaattat tcgaaattat caatattaga caagttcttg agcaagcaaa atcattaaag
    19861 cattttttca aattagacga aaaaaatcaa tatccatttt taaataaaat tgtaaatcaa
    19921 ataagttttc caaactattt atatgataga ataaatagtg tagtttctaa aaatggaaca
    19981 ataaaagatt ctgcatctca aaatttgaaa gagataagaa aaagtttgaa tgacactcaa
    20041 accaatatta ctaaaaagct aaacaatgta ttatctgcat ttaaaaaaga gggttgggta
    20101 agcaaagatt tgtcggcaac ttatgtaaat ggacgtttgg ttttaccaat agaaactatt
    20161 cataaacgaa aaattaaagg tttaattcac gatgagtcgg caagtggaaa aacatcttat
    20221 atagagccac aggaagttgt tgagctaaat aatactttga gagaacttga aattgcagaa
    20281 aatagagaaa ttcaaaaaat cttatttgaa cttactgatg atttcaaacc ttatcgcgaa
    20341 gagttgctta cattatatga aaatattgct tatttagatt ttgtttgggc aaaagcaaag
    20401 ttttcgctta gaatagaagc tatagtgcca aaaatagata aagacccagt tttagattta
    20461 cataaagcaa aacatccact tttattttta aactttaaaa aagaaaaaaa ggaagttgtt
    20521 cctagcagct tttatttgaa ttccgaaaat agaattttat taatatcggg accaaatgca
    20581 ggtggaaaaa gtgtttgtct taaagctacg gcactattga cttatatgaa tcagtgtggc
    20641 ttgccaacgt ctgttggtgg taatactgtt atgggaattt ttgataaaat ttttctcgat
    20701 attggcgacc agcaatctat agaaaacgac ttgagcacat atagttctca tttgttgaat
    20761 atgaaatatt ttttaaaaaa ttctgacagt agaactctta ttttaattga tgaatttggt
    20821 tcaggaaccg atccaatatt aggcggaact ttagccgaag caattcttga agaactgaac
    20881 actaaaaatg tttttggact aataacaact cattattcta atttaaaaat ttttgcttca
    20941 aacactccgg gaatatttaa tggtgcaatg cttatagacc aaaacaaaat ggaacctact
    21001 tttattttag aaacaggaat tccaggaagt tcttatgcca tcgaaattgc aagcagaata
    21061 ggtttagacc aaaaaattat tgaaaatact aaacttaaag caggagacga gcaaattaat
    21121 attgacaaat tcctgagaag attgataagg gataaaaaat attgggagaa aaaacgtcaa
    21181 aaaattagaa tacaagaaaa aaagattgaa gaattagttg agaaaaatat tgaagaatta
    21241 gaaaaattaa aaactagtag aagacaaatt ttagattctg caaaaaaaca gtctgagcaa
    21301 atgcttgctg acgtgaatag gcaaattgaa aatactatca gacaaataaa ggaatcaaat
    21361 gcagataaag aaattagcaa aaaagcccga caagaaattg aagaatttaa ggaagatttt
    21421 aaaaattcat tgtcaaaaga acaagacgaa atagaaagaa aatacaatca ttataaaaag
    21481 aagcaaaaag aaatagaagc taaaaaagct gcaactcaaa aagctaagcc agaaatcatt
    21541 aaaatagatg agaataaaat taatattggt agtaaagtga gaataaaagg tcaggaagtt
    21601 attggcgaag ttattgattt gggcgaaaaa aatgcggtag taagttttgg aaatatgtat
    21661 tcgagtatag cttttgagaa attggaaaaa gttagcgaaa cactttacaa gcaacaaaat
    21721 cgttcggctt ctaacttgca ttttaattat aatgaaaaag ttttaaattt caagccttac
    21781 atcgatgtta gaggcgatag agtagaagag tgtcttaaaa aaatttctaa cttaattgat
    21841 gaggcagtta tgttgagttt taaagaaatt aaaattttac atggacgcgg aaatggaatt
    21901 ttaagacaat atatacgtga ttatttaaga accttacccg aagttgaaag ttttcataac
    21961 gaagacatta ggtttggtgg tgatggaata actatagtaa aacttagata aatgattgat
    22021 attgaggaca taatagttca ttatgatatt tttaaagaat gtttttgttg cgatttagaa
    22081 aaatgtaaag gaatttgctg tgtagaaggc gattcaggtg cacccataga agttgaagaa
    22141 gagattaaga taaagcaaat cttacctatt ataatagatg agcttactga tgaggcaaaa
    22201 aaaacaatag aagagcatgg tgtttctatg gttgatatag atggagattt aacaacaagc
    22261 atttgtggta acacaggacc ttgtgtgttt attcaaaccg atgaaaacgg catagcttat
    22321 tgtgtaattg aaaaatattg ggaaaaaggg cttatagact ttagaaaacc tatttcttgt
    22381 catttatatc ctttacgaat tagcaaatac agcacttttg aagccttgaa ctatcatatg
    22441 tgggatattt gtgcagatgc tagagtttta ggaaaaaaac ttaatttaaa gatttttcaa
    22501 tttttaaaag aaccgcttat aaggaagtac ggagaagagt ggtacgaaga tttgttagtt
    22561 gcagcaaaac tttttgagga agaaaataaa gaataatttg ttttttcttg taaaatattt
    22621 tcaagtttaa taattttgca cgcgaaaata atttttcggt ccgtagctca gtaggtagag
    22681 cacgtgactc ttaatcatgg ggtcgggagt tcgatcctcc ccggaccgac aaaaaaaaga
    22741 gctgtcttat ttttgagaca gctcttttca tttattcatt tacaaaaatt aattatcttc
    22801 caacaaacat tccaaatttt actttagttc ccgattgagt gtaaatgtaa tgtctgccgt
    22861 taaaatcttt aaaaaattct ggcattattc taaatttctt gttctttttt gtaaacaagt
    22921 aatctccatg cattttgcca tctgagaata tagaaactgc tacagcaact cctcttctgc
    22981 ttggagaagc aatttcttta aggtcatttc ctgttagctg tgagttttgt agaagtttga
    23041 cgtttttagc attgtcgtta tagaaaacaa atattttttc tccaatagca aacgaagcaa
    23101 tagacgagaa ttggcctaaa tcgttgtagc tatattgtgt tttaggaatt cttgtcatcc
    23161 attccatatt attttcaggg ctacaataag caactaaaac atcattaaag cgataatagt
    23221 cattatatag aatttctttt gttccaggaa caactattga gtcaacccaa tggttttgat
    23281 gttcggcaat aacggcagaa cctccgttag acaaatgtaa tacatctatt aatttatagt
    23341 tgtacagttc tgaataattt tttgctaaat gaggagctct aaattcagga acttctgttt
    23401 ttgaaaaaac ataatctgct tttttgctat cgccagttac aaaagtttct gttgttggat
    23461 tgtatttttg atggaaaatt ccttcataat ctgtttttcc ttttcttacc ataaatccaa
    23521 agatgtcgat gttttcttct tcgtcaacac caattatagc atcaactaaa acatgttttt
    23581 tacctttaat atcaacgtct ttaacagttc ctgcgctaac attaaaatac aaaagtttat
    23641 aatcataaat aatagttttc attcttttga gttgtcttgg gtctgggcta actctagcaa
    23701 gcatatatac atttccagaa ttagcgattt ttatttgttc tatctcaaaa gcttgattta
    23761 ccaaaggtaa ttttacaata ttattgtaaa tttctctcat gtctgggtca taaactttaa
    23821 agaagaaagg ttcttcgttg taagtttgga aaggacgatg gtaatacaca aaaatatttt
    23881 gttgattatc cattaattct acatgaaaat ctacgctcat attttggttt gtaagttttc
    23941 ctatggcggt tgcttctcca ataacttgtc ctgtacggtt tacgtgctgt atgtataatg
    24001 ttttttcttt tctagtatta tcaactactt gaaagaacaa aataagttta ctgtcaagat
    24061 aaaacatttc aacatattct gattgcatgc cacctaacat aggtaaaata agttgattac
    24121 tgttttccct actcatagaa gtattgttgt aaaagtcaag atgtaaagca tctttttcat
    24181 caattcttag agcataaaat ccgtcagagt cggctccgat aagtctaaca tactcgttca
    24241 tagcgggtaa aacaatttct ttatcatacc caaaaatggt agaaacctgt gcattagtaa
    24301 gctttgcaaa agctataagt gctaaaagta taagaacaaa ttttttcata atctttagtt
    24361 ttatagagtg ctaatataga tatttttatt taattattaa aatttttatt tttatttgca
    24421 agttgtccgc aggcagcact aatgtccttg cctttagact ttcgcaaagt tacaataaga
    24481 tttttatttt caataaaatt aatgaaatct tctgtatttt ttttgttaga agccgtaaat
    24541 ttgctctcag aacttggatt atattctatt aaattaattt tacaaggact aatttttgta
    24601 aaatctgtta gtttttttgc atcttctaaa gaatcattta agcctccaag caacaaatat
    24661 tcgtatgtta ttcgtttttt agttttttga taataatatg aaatagcatt ttttagagta
    24721 tctaaatcat attttttgtt aattggcatt atcaaatttc gcttttcgtt atctgccgaa
    24781 tgtaaagata ttgataagtt tatttttaaa ttatcatcag caagtttttt aattccttga
    24841 gcttctcctg cagtagaaag cgtaattctt tgaggcgaaa aatgcagtga gttttcgtgg
    24901 cttatatgag ttaaagcctt tatggtattt tcgtagttta aaagcggttc gcccatgccc
    24961 ataacaacaa tatttgaaag ttttctatta aatatttgtt cactcatttt atttaactga
    25021 aaaatttgct caaatatttc gccagcactt aaatttcttt taaaaccaag ttttccagtt
    25081 gcacaaaaac tacaatttaa aggacagcca acttgagtag aaatgcaagc agtaactcta
    25141 tctttattgg gaattaaaac tccctcaaag ttatgactat cagcagtttg aaataggaat
    25201 ttgacagttt tgtctttact ttgttgtttg aaaataattt ctaaaaaatc tatgaaaaaa
    25261 ttttcgtcta aaaaatctct aaaagctttg ggtaaattag tcatttcttc aaaatttgaa
    25321 gccgatttat accacaacca ttcataaact tgatttgctc taaacttagg ttctcccaaa
    25381 tttgcaaaat tagcaatcat ttgttcgtgg tttaaggctc ttatgtttat ttttgaagac
    25441 atgaaaaata aagatttata aaaatatata catcaaaatc ttaaatataa tacaaaatta
    25501 ataaatgttt ttgtacttgc taagttttaa gggaaaattt tgtattttga aattttccct
    25561 caacactcaa caattcatag agctacaatc gaaatatttc tatactttaa ataccttctt
    25621 ttgttccttt tgtgtcttaa aactaaaaac ttaacactaa ttatcgggtg tcgagctaag
    25681 ccgaacgggt gttgagcgaa gtcgaaacac aattcttaat taattaccag ttcatcaccc
    25741 tgaaattcca cttttatttt cttagagtcg ctagttccgc tgattataaa cttagcaatt
    25801 tgattttcta attctttttg tataaatctt ttaactggtc tagctccgta ttgtgggtcg
    25861 taagatagtt ttgcaatagt tttaatagag tcttcagaaa tttcaagctc atatcctgct
    25921 tctgttagtt tgttttctag tttcgacaat tgaagtttta ctatttcata aatttgctct
    25981 aaacttagag gagtaaacat aattatttcg tcgattctat ttaaaaattc tggtctaaca
    26041 tgtgctttca aggcttctaa aacctgagct tcggtttctt tataattttc gtttcctgat
    26101 tttatttgct cttgaagaaa ttgcgaacca atattggaag tcataattat aatggtgttt
    26161 ttaaaattca cagttctacc tttattgtca gtaagtcgcc catcatctaa aacttgtagc
    26221 aaaatgttaa aaacatcggg gtgtgctttt tcaatttcat ctaataaaac cacagaataa
    26281 ggtttacgtc taactgcttc agttagttgt cctccttcat catagccaac atatcctgga
    26341 ggagctccaa ctaaacgtga aacgctatgt ttttcttgat attcgctcat gtcgattctt
    26401 gtcatcatag attcgtcgtc gaacataaat tcagccaaag cttttgcaag ttctgttttc
    26461 ccaacaccag ttgagccaag gaagataaaa gaaccaatag gttttcctgc atcttgtaat
    26521 cctgcacgtg agcgacgaat agcatcagct acagcagtaa ttgcctcttc ttgacctatt
    26581 actcttttat gtaattcttc ttctaaattc attagctttt ctctttcgct caaaaccatt
    26641 ttgctaactg gaataccagt ccatttgcaa acaacatcgg caatatcttc agcaccaaca
    26701 acttcgtcaa ccaaagcatg ccctccttgt aattcttcta atttggtttt aagtttttca
    26761 atattttgtt gactttcagt aatttttcca tatctaaatt cagctacttt tccataatcg
    26821 ccactgcgtt cagcttgctc agcttgaatt ttataatttt caatattttg cttttcggct
    26881 tgaattttat caattaaaga tttttcttct tgccattttg ctgttaatac attgcgttct
    26941 tcgtttaaat cagataattg tgtcgcaatt tcttctagct ttttggtatc gttttcacgt
    27001 tttatggctt ctttttcaat ttctagttgt tgaattttac gatttaaaac gtcaatttct
    27061 tcgggaacgg aatttacttc caatctaagt tttgaagccg cttcatcaat taagtcaatg
    27121 gctttatctg gtaaatatct ttcagtaata tatctatgcg ataattcaac tgctgcaaca
    27181 atagcctcgt ctaaaattct aactttatgg tgattttcat atctttcttt taatcctcga
    27241 agaatagaaa ccgagcttgg gatgtcgggt tcatcaatca taacagtttg aaatcttctt
    27301 tctaaggctt tatctttttc aaaatatttt tgatattcgt ttaaagttgt agcaccaatt
    27361 gcttttaatt cgccacgagc aagagctggt tttagaatgt ttgcagcatc cattgcacct
    27421 tgtgtttgcc cagcaccaac taaagtgtga atttcatcaa taaataaaat aatttcgcca
    27481 tcagctgcaa caacttcttt aattacagat tttagtcttt cttcaaattc tccttgatat
    27541 ttagctccag caattaacaa acccatgtct aaggaaaata cttttttgga ttttaaatta
    27601 tctggaacat cacctttaag aattctgtag gctaaacctt cggcaatggc tgtttttcca
    27661 acgccgggct caccaattaa taaaggattg tttttagtac gtctcgacaa aatttgcaaa
    27721 actcttctaa tttcatcgtc tctaccaatt actggatcta attttccttg ctgagctctt
    27781 tctattaaat ttacagcata acgatttaga gcatcataac tgtcttcagc tgattgtgag
    27841 tttacttttg aaccagctct taaatcagaa attgctttct ttaaaaaatc tttttttact
    27901 ccagcatcac gcatcatctg tccgataatg tctttggatt cagataaagc caaaattaaa
    27961 tgttcaatag aaataaactt gtcgcccatt tctatcttat gcttgtttgc aagattaaaa
    28021 acatcttgag tgttagaagc tagataaact tcgccacctt caacttttgg ataagagttt
    28081 acaatactta aaactgccga ttcaaaattg tgcttatcta tttctaattt agacaaaatg
    28141 attgagaaaa tattatcatc atcatttagt aatgcaagca aaagatgacc tggctcaata
    28201 gcctgatttt taagctcttt tgcttttatt attgaagtct gaattagaga ctgtgctttt
    28261 attgtataat cattcaaatt cataatatta ttgttttaaa aatttctaat ataaattctt
    28321 caatttatga accaaacagc acaacaggac aaattgacat gctttctttt gttgtttgcg
    28381 acaatttgac agtttgtgtt tgtgaaatga tttaacaacg gtataaaaat cttataaaaa
    28441 acctgaattt tagcaaccaa aatgcaattt tttagtctaa aaataagtta aaatgatggc
    28501 aataaataaa taaattaaaa aaaacgaata attttaatgt attttaaagt atttttaaat
    28561 agatttatta actttgtcaa attaataatt gtaaaaactt tattatagcc ttatgagaaa
    28621 gatactctca acatttataa taattcttac gcttgggttt tttaacaaca tatttgcaca
    28681 agctaattgt ggtgctgcag tggcaattcc tttagaagta tatggttctt gcggtgatat
    28741 gtttttcacc aatgttgact ttactgcggc aacaacttcg actgatgcac cagcaccaac
    28801 ttgtggtggt tttaatacag caaccgaaga tatgtggtat agttttgaag tgcctgctgg
    28861 tgttactgaa ttagcttttc acgcatttaa ttcgccagtg ccattgccaa tggttagtcc
    28921 agcttgcgct cctggtatgg ctgtttatag aggaacttgc ggtaacttaa cattgataga
    28981 ctgttttaat gctgatgcag gttttatggg aatatcaaat ggtgagattc gttgggaagt
    29041 attaaatgtt acgcctggtg aaactattta tgttcgtttg tgggaaaaga ccaataatga
    29101 aacttctata ttttttgcag cctctgtaat aacatcttta ccagaagctg attgcaataa
    29161 tccaccagaa ttaggtacta cgggctgtaa tattttagct ccttccggaa ctatccaagc
    29221 tcctgatgat tgtggttgga caactactga taatgtcgtt ttttattctt ttgaagtttt
    29281 accaacagac ccacagccgg tagttattga aattgaatat ggacaatgtt ggggaaacga
    29341 aactggagga ttattaccaa gtaatcccga aatacaattt gctgtttact cttggaatgg
    29401 agtaaattgt aatggaattg gaggtggacc aacaagcgac ccagcaaata ctactacata
    29461 ctatggttgt gaaaatggtg taggaacagt agtttattca aatactttgc cacccggtca
    29521 atatgttttg gctatggacg gacatagttt accaagtgga aattctcttt gtacttttgg
    29581 aatagcagct tcttttcttg atgaacctcc tattactgga gagctttctg taagtctttc
    29641 aaaagtaaat gttggttgtg gacaacttgg ttcagcaaca ataactataa atagtgcttg
    29701 ttctacaaat ccaacaataa attggagtac tagtgctact ggcactacag taaataacct
    29761 tactgttgga gcttattctg taacagtttc agaccaagct ccttgtacag atacaataat
    29821 taattttaat attgcagacc aaagtaattt tgcagttgca attactactt caggaaatcc
    29881 ttgtactggt cccgttacgc ttcttgcaaa cgttatgggt gctaatcctg ctaatgttga
    29941 ctttgtgtgg agtcctggcg gacaaacaac tcaaagtata acagttaaca atggtggcac
    30001 atatactgta accgcaactt atggaacttg tgttgacgat gctagtacaa cagtgacaga
    30061 tggagatttt gattttagtg taaactatac tccaactttc tgtgcaggaa gcagtggagc
    30121 agctcaattt aacttaattt ctggaactgg tccttttatg tatgaatggt caactggggc
    30181 aattagtcct ggtatccaaa ttacagaacc tggtaattat tgtttaacag ctactgactt
    30241 gaacaatgga tgtagtttga caaaatgttt taatgtaata gaacttcctt ctgttgatgt
    30301 aagtattgta tcaaaagata ttacttgtca taatgcaaat gatggagaag ttacagcaat
    30361 agctagtggc ggaacatctc catattctta tgaatggaat ggatatattc caggcgaaac
    30421 tatgacgaat ttaggagcag gtaattattc tgttgttgtt acagatgctg ctggatgtac
    30481 tggttatgct actacatttg taaataatcc tccacaattt acttatagca tttctccaaa
    30541 ccaaggtata tgctatggcg agcaagcaga aatcagtgtt attgctaatg gaggagtaga
    30601 gccttatact tatgtttggt ctgatgaacc tactaataat attgacacta ggattgtaac
    30661 tccagatact actactactt attgggttac tgtttacgat gctaataatt gcatgtatcc
    30721 accacaacaa acaaaagtga ttgtaagttt gccaagaaat atttcagtta ttgttgacaa
    30781 tgttttatgt catggggtgt gttctggtag tgctgttata gaaattactg gaggtgttcc
    30841 tccttttaat ataagttgga ctagtagtga tgatagcgat atttcttatt ctcatactat
    30901 cccaaataga acagattctt gggctagctt atgtgcaggg aattatgcag tttcattaac
    30961 agacaaatat gaatgtgagg gaagtgctgt ttttgcaatt acagaaccag acacaatgta
    31021 tataagcaca ctttcgggta atgcaacttg ttttggatat gcagatggtt ttgtgcaagt
    31081 tgaagcaact ggtggtgttc cttatacaaa tgaatttggt tcgttttatg aatatcactg
    31141 gagtaatgga tttagtgcag attctcttgc agtaggttat ggtatttatt atgtaaccgt
    31201 taatgatgca aatggttgtt cacatgttgg tatggctttt gtcgatcaac cagacgatat
    31261 ttatataacc gctccttgga atggacaaat ttgtatagga gaaccttttt ctacacatgt
    31321 tgcagcaaca ggaggtactg ggccttacga gtttatttgg tacggtagtg ataattctat
    31381 ttggtatgga gatggtttaa atgtaagtcc cgaagttacc acaacttatc agctagttac
    31441 tatcgactct agagggtgtt ctggacctgt tcaaactgtt gtggtaaaag tacatttgcc
    31501 aataaaaatt aacgaaataa gcagttctaa agattatatt tgtttgggtg ataatcttac
    31561 agtagaaatt gaatatgaag gtggaaatgg tggaccatat acaattacat ctccacagca
    31621 aggaatagtt aatactcctt atacttttag tcctgatagt acagggtatt atagctttac
    31681 agtagctgat gattgtggtt cgcctgtgga tacagattct atttatgtag ttgtacataa
    31741 agctcctagg gttgcatttt ttgcagacaa aacaagttct tgtcctccag gaacttttca
    31801 tttcacaaat actaatgaag atcttaataa cacatattta tgggattttg gcgatggagg
    31861 attttctgta gaaaaaaacc cagttcatac ttacgacaaa acaggtaatt atacagtttc
    31921 tttaacagca tggacagagt ttggttgcaa aaaaactaaa acttataata acctaatatt
    31981 tattcatcca aaaccacgag cagaatttac agcaagtcca gaattagttt ctattttaaa
    32041 tgcagaaatc acatttgata actttacaga aggtggaact tcatatttct gggactttgg
    32101 cgatggagaa actccagatg cttggtcgga agataaagaa ccaaaaaaat acacatatag
    32161 atctgttgga gaattcgata ttactttaat caccaaaaat caatttgagt gtacagattc
    32221 tattcacaaa aaaattagag ttcatgacga atttacattt tatgctccag aagctttcac
    32281 accaaatggt gacggaatca atgattattt ccatttaatt ggacatggaa ttgataaaaa
    32341 gcaattttat atggttattt acgatagatt tggaataaaa gtgtttgaaa caaatatatg
    32401 ggacgaagaa aatccttata gaatggcttg ggacggcact cacaacggaa gtgtagaaaa
    32461 tggagataaa atcctgccaa atggaatgta taggtggtat gcaagttttg ttgactttac
    32521 tggtaaacct cacgaagaaa gcggaacggt tactataata agataaatgc aattttctca
    32581 aaataaaatt tgagtttgag cgttttaaag aaaatttttg gaatgataaa aaaaatcgca
    32641 ttaattctta gtataattgt tagctttact tttgtgtggc ttattaatat ttcatgtgag
    32701 aaaaataatt ttactacaga ccctgaagat aagttaattt tttcaaaaga cacagtgcag
    32761 tttgatacag tttttactca aaaaggaagt gctactagat attttgttat taaaaactca
    32821 aacaaacaaa aaccaatttt aattaataaa atttttctcg ctggtggcga ccattctcct
    32881 tatcgaataa atattgacgg tttccctgta aataatctaa acaattatga gttagaaaga
    32941 ggagattctc tttttatctt tgtcgaagtt accattaacc ccggacaaaa cgatatggtt
    33001 gaagaagatt ctatagtttt tgtttcaaat ggaaatgtac aaaatgtaaa actaatagct
    33061 tttggtcaaa atgttacatt gctaaatagt aagtttataa gtaatgacac tacatggaca
    33121 gatgcaaagc ctgtattaat atataattct gcattagttg aagaaaatgt tactttgcaa
    33181 gtaactccgg gagctaaggt ttattttcat agaggagctt ctttatttgt aaaaggaagt
    33241 ataaaagtta atggcgataa agataatcgc gtattattta caggagatag gttagaacca
    33301 tattatgccg aacttgcagg acaatgggga gcttttctcg aagatgctca tagaaataca
    33361 ataggaattt ttggaggaat acatttgtta gctggctcaa aaaattccga aattaatttt
    33421 gctgatataa aaaatgcaat tataggttta caagtcgatt cttgtgttac tccagggact
    33481 ccaacagtaa gattaaattc tacaaatatc gaaaattcaa aaattatagg tctgtatgca
    33541 cttggctcaa ctattgaagc tgaaaattgt gtttttgcaa atagcggaca gtataatgtt
    33601 gcttgtttga taggcggaga ttatagattt ttacactgta ctatggcaaa ttattggtta
    33661 gaaaatcgac aaacaccaca acttatttta aacaattatt ataactatcg tgatgctaat
    33721 ggcaatattc aaacttcatt tagagatttg cacgatgctt atttcggaaa ttgtattatt
    33781 tatggttcaa aagataatga gataaaattg gattttgaca atagaagtct ttataatttt
    33841 agatttgata actgtttgat aaaacaaaaa gattttaaag attttgaagg aacggatttt
    33901 tttatcgata atatttggaa cgaaaatcca aaatttaaag atattataaa gccttttgat
    33961 tatgaattag atactctttc tcctgcaaaa gatgtgggaa aattaagcat agcaaatcaa
    34021 gttcccttag atcaattagg caacaatagg ttgttagatg gaaagccaga tttaggagct
    34081 tacgaaagac aagaataata agtttgcata aaattgtttc aattaaaatt ttattactaa
    34141 ttttgagggt aatattttaa ttatgaaaaa cctttgttta ttaatagtat taagttttgt
    34201 gtttttgcta attggttgta aatcaaccaa agacacagac caagcaagaa aaacttacaa
    34261 tcctgaaaat gtaaattcag actttagaat aatgttttat aatgttgaaa atttatttga
    34321 cactgaaaac gacccatata aaaatgatga tgaatttact ccagacacag gaagatattg
    34381 gacaaaaaca agatataacg aaaaattaag aaatatatat aaagtaattg ttgccgttgg
    34441 tggttgggat ttgcctcaaa ttgttggttt gtccgaggtt gaaaatttaa aagttttaaa
    34501 agatttaata aattacacac cactttataa atctgattac aaaattattc actacgactc
    34561 tccagatgca cgtggtattg atgttgcatt gctttataga gaaaattatt tttcagttat
    34621 atcacataaa cctatacctg tagtttggcc caaacatatt ggaactggaa ctactcgcga
    34681 tattttatat gtttgtggag taaccaattt agaagacaca ctgcatgttt ttgtaaatca
    34741 ttggccctca cgttggggag gacagataga aacggaagaa aaacgtatgc atgtagccaa
    34801 attgctaaaa cattcaacag attctatttt taaaattaat aaaaaggcaa acattgttgt
    34861 tatgggcgac ttaaatgatc atccaacaga tagaagccta acagaatctt tacaagcaca
    34921 aactaatttt gataaaataa aaagtaataa attatataat ttatcatatt atttacaagt
    34981 tgaaaaaaaa cttggaactc ataaacatca agatcaatgg ggaattattg accaaattat
    35041 tgtgtcggga gcattattaa gtggtgaagg aaaaatatat accactttgg acgatgctca
    35101 tgtttttaac gaagactttt tattggaaac agatcaaaaa tttactggaa aaaaggtaaa
    35161 aagaacttat attggtttta aatatcatgg aggatatagc gatcacttgc ctacatatgt
    35221 tgatttaaag caaaataaag ataaggttgc ttctgaataa gtaattaatt gaaaaaatac
    35281 agaaaaattt tcaaacgtat ttgaaaataa ttatatttgt agaaaatttt caaacatgat
    35341 tgaaataatt aattccatac gcaagtataa tttttgggat tttaatccta tagatttggg
    35401 ttttgctcga acattttaca ctaataaaat tggtcaatat cttggaaaca aattagtaaa
    35461 agtgcttgtt ggtcaacgcc gtacagggaa gagttatatt ttacgtcaaa ttgcaggaaa
    35521 cttactgtcc gaaggagtaa aaagtgaaaa tattctttac atcaataaag aatatataga
    35581 gttaactggt ttacggaatg cagttgattt ggagcaactt tataaaactt atcgtgagac
    35641 cttaaagcct aaagggaaag tttatatttt tattgatgag atacaatata taaatgagtg
    35701 ggagcggttt gtaaattcac attcacaaga ttttgcagag ccttgcgaat tgtttatcag
    35761 tggttcaaat tcaaatcttt tgtctggtga gttagcaacg cttttatcgg ggcgatatat
    35821 tgagtttgag atattaccct atagttatat agaattttgt gaacttactc aacaagaaat
    35881 agggagtaaa acttacaaga attttcttca aagtggtgca ttaccagagc ttttcaattt
    35941 gccaaatgat gagatgaaac aaaattacat atcgtccata aaagatactg tgatgttgcg
    36001 agatattgtt gcgagatata atgtaaaaga tgtaaaactc ttagatgatt tatttgttta
    36061 tttggttaat acagcatcaa atttgatttc aataacaaat attattaatt tttttgcttc
    36121 aaaaaatcgt aaaactaact atgaaacttt gtcatcatac ataagttatt tggaaaattc
    36181 atttctaatt catcgtgtcg aacgctacaa tattaagggg aaagatacaa tttctggcaa
    36241 cagtaaatac tatattaatg atttaggcta taggaactat ctctactctg gaaatggtta
    36301 tggaattggt tatcttttag aaaatgctgt ttatttgagt ttgagacgtg ctggttatca
    36361 agtttatgta ggaacaatac aaaattctga agttgatttt gtagcaatta aaggtgaacg
    36421 taaaatttat ctacaagcaa cacttcagtt gacagaagaa aaaacaatag aaagagaata
    36481 tcgtgcgtta aaacttattg aagataattt tgataaatat gtagtaagta tggacgatta
    36541 taaaattcct agcaatgagg gaattgaaca tatttcagca tggaatttag aaaatgtttt
    36601 aaatagttta taaaggcagg caatgaacaa gttttaatta aaaattatga aagaaaattt
    36661 gaaacttatt tcgactgagt atttagaaga atactcgaat aaagtaggtg ttgatttgtt
    36721 tgaaatattc aataaattgg aaataaaaaa ccaattttca tttgaagatt ttgagtatta
    36781 cataatttca tcgtctttgt attcatcaaa aatagagggg aacacacttg atgctaatag
    36841 ctttttcagg agtaggggca aaaaaacttc tccaaagaaa aaagaagttg aagaaataga
    36901 aacacttatg gaggcatatg tatttgcttc agataataaa ttgaatagaa ctaatttttt
    36961 aaaatcccat gccattttat caaaaacatt tttaccaaca aaagaaagag gagttttaag
    37021 aaaagaacaa gtaggtgtga gggattccaa aacatttaga cctgtatatt tagctgtaga
    37081 acctgaattt ttaaaagtag aatttgataa ttttttcgat gatattgatg aattgttaaa
    37141 aagagatttg agtcatacag aaatttttta ttttacttca atgcttcata tttggttggc
    37201 tcaaatccac ccttttacag atggtaacgg aagagcagca cgattattag aaaaatggtt
    37261 tttagtttca aaacttggaa agtcagcttg gtcaattaat tcagaaaaat attattggga
    37321 caatcggact gattattatc aaaatatttc tcttggatat aactactatg ctttattttg
    37381 ggaaaggtgt atttctttct tgttaatgtt gcctaaggct gtaggtttat cgtaatatgt
    37441 ttgcttacaa tatttaattt tgcgtatgtt tattaaattt ttattgtatt tattcattta
    37501 ataaccaatc aattatgttt atttttttta ttccattgaa gtaagttatg ggttctacat
    37561 cacttgtgat tagtgtacgc ttattaaaat cttttataag ttcaaatgat ttaatttctc
    37621 tttcgtaagt gtttttttct tttgccgtcc aagcaacttg aatgtattcc atattgcctt
    37681 ttggattacg aacaacaaaa tcaacttctg tattacctgc tttgccgata tttactcgat
    37741 aaccacgtct taatagttct aaaaatacta cattttcaag attatgacct aaatcaagat
    37801 ttgctttatc gcttaaataa tactttttaa atcctaaatc aacactataa tatttttgct
    37861 gtgttgctaa tagtgctttg ccttgtaagt cataacgctt gactttgtaa aatagatatg
    37921 cttctgttag agcattaata taattcccta ctgtgttgtg tgacaatgaa atagcatttt
    37981 cggttctcaa tatgtttgta attttttttg gagaaattac agagccaata ctatcgaaaa
    38041 gaaatgctgt tgttttgtta aaatggtctt cgttccgcca attataacgt actaaaatat
    38101 ctttttgaat aatgttttga attacttctt gaatatagtt ctgtacagca aagtctggca
    38161 attcgaaaat tcctggcatt ccacctgtaa ccaaatattt attgaacatt aagtcggttc
    38221 gagcattttc tggttgtgta tataaaaatt ctttgaacga aaacggtaaa acatgaatag
    38281 aaatatatct gcctgtgagt aaagtgccaa gctcactaga aagtaggtaa gcatttgaac
    38341 ctgtaatata tacatcaata ttgggcttta cataaagtcc atctatgagg cgttcaaatt
    38401 cttccacttg ttgaatctcg tcaagaaaaa cgtatgactt tttttgggga tctagtcgtc
    38461 caattatctc tttatacagt cctttgtaat tttttagcca aatttcgtta tctaaatcct
    38521 caaaattaag aaaaacaatt tgtttttcac ttacgccgtt tttgagcaga tagtctttaa
    38581 acatttgcat tatagtagat ttaccgcagc gtctaacacc agtaagaact tttattagat
    38641 tggtatcttt cagcgcaatg agttgctcta tgtattgttg ccttagtatc atgtttttaa
    38701 attttataga ccaaactatt gaaattttag aagtttggtc tgtaaaatta atgtttttta
    38761 tcgataaaag caaatgattt ataattatct aaaatttatt aattataaga ttttacaaga
    38821 aaaatttaaa ccttcgcaat caccgaaatt cttattgatt ctgattttcg tcttcgttta
    38881 ttttaatatt aaatttttca gcaacttcat taaatagatc aagcattcct ttttctttta
    38941 gctctttata catcaaagaa attaggtatt cactatcttt tatatagtca gtattattag
    39001 tgttaacata cattatatgt gcaataaaaa cattttcaag agcatttttg taatcagact
    39061 ttaaaaatag aatcttgcct gcaccgaagt aaccttctgg gtcatctgca taaaggtatg
    39121 ttagaaaatt gtaataattt aatgatttgt cataatcgtt cataaacgaa taaatcacag
    39181 cagtatttaa aatagccaaa tagccttcgg gataaatgtc cattgatttt tggtagtatt
    39241 ttaatgcttg tttaaacttt tctaatcttc tatatgatat tgccaaatga tcgtatgcta
    39301 agacaaaatt ttgtcaattt taacagcttt cttaaattct gatattgatt ttttataatc
    39361 gcctttatcc ataaaataat ttccagtgct gtagtgttca tttgcttttt gatttgatga
    39421 gagttgataa aattcgcttt ccttttcgtt aataattaat cgtcttacgc tatagcaata
    39481 tgaataaagt agcttgccta cttcttcaaa agccacgtta acaccatcaa cagtattaag
    39541 aatttccatt tcgtctgctg ggttttcatt taaaacacgc gacattgctg ctgatacgca
    39601 atctacaaca ctatcttcta attcttgtaa tgtttctaat gagctaaaac aatcacatga
    39661 tttgattgcc accttttcgt aaaatgtctg tgcttttata catgtggcta gtaaaaaaac
    39721 aatgataaag actttaagat gtttcatatg tgttatgctt tttttataaa tataaatgtt
    39781 ttgcggagaa taacgcactt attcttcgca ttttttgcgg agaaaataaa tttgaaaaaa
    39841 taaaacacag caaactaaaa aagctctaac ttaataatat tagagctttt ctttactaga
    39901 gcggagagag ggggattcga acccccggta ccgttgccag tacgtcagtt tagcaaactg
    39961 gtggtttcag ccactcaccc acctctccag ttgcgataat atgagattgc aaaggtagtc
    40021 cttttttaca tatttcaaaa ttatttgata aattatttaa gttagagtgt tttttttatt
    40081 tttgtaaaaa ttttataatg aaacgagaaa tagttttagc ttatataggt ttggctgtga
    40141 tgacccttat tatagggttt tcatttattt ttgtaaaaac aggcttaaac tattctaatc
    40201 cctacgattt gcttgcccat agatttaata tggcgttttt ggtaatttta atccttttaa
    40261 ttttcaaaaa aataaagttc ccaaaagtct catttaaaaa actgatgaaa cttattttaa
    40321 ttgcaatgtt ttatcctgtt ttgtgctttg gattgcaagc tattggcatg gtaaactcaa
    40381 cagcttctga agcaggattg attttttcat ttttgcctgt aataacatta gttgcaggaa
    40441 atatttttct taaagaaaag tctagcactg ttcaaaaaat tggagtgctt ttatctgtat
    40501 ttggaatagt atttattttg tacttcaaac aaggactatc taactttaat gtacagagca
    40561 ttatctttct tttcctttct gtattttcta tgattggcta ttttattttt ggcaaaaaag
    40621 aaatttcgaa aatcgattct ttaagtttaa ctgctataat gattagtttg ggatttgtgg
    40681 tttttaatgt agttagcatt tcgcgacatt tagcaataaa taaagattta aacttatatt
    40741 tcgaagcgta taaaagcagt caatttatta tctcggtagt atatttggga gttttgtctt
    40801 ctgtattaac ttctttttta tcaaattatt ctttaaatta tttgccaaca tttaaagtaa
    40861 gtattttcag taatttgaat ccaataattg ctattgtagc aggagtttta atcttaaaag
    40921 acgattttta ttggtttgat tttattggtg taagtctagt ttttattggt gttataatgg
    40981 tgttgtttta taaaccaaaa caaaagcaag caacagttta ggtttgtaag tttctaattt
    41041 tcaaacacgt aattaattaa atttgctccc attttaagag cttttaatct tgtttcataa
    41101 gaattgttgt gtacttcttc gtcttcccag ccgtcgccta agtcggtttc gaaagaataa
    41161 aaaacgacaa gtctgccttg ataaattatt ccaaaacctt gaggtggctt attatcatgt
    41221 tcgtgaattt ttggcaatcc ttttggaaaa tcatattttc gatgatatat ggggtgggag
    41281 aaaggtagtt ctataaattc actttctgga aaaacttttt tcatttctct tctaatatat
    41341 tggtctaaac cataattatc atcaatgtgt aaaaagccgc ctgcaattaa ataattacgt
    41401 aaatttctcg cttcataatc agaaaacaca atgtttccat gtccggttaa atgcacaaaa
    41461 gggtaattaa agatatctgc acttcctggt tctacagtag ctggttcggg gttgatattt
    41521 gtgcctaagt tagaattaca aaattttatt aaatttggaa gcgaagttgg atttgcatac
    41581 caatcaccac cgccactgta ttttaaaaga gcaattctgt aagtttgtga aaaaccaaaa
    41641 ttaaaagcaa gattaaataa tattacaagc aagaaatatc tcataattta ttttgaatac
    41701 aagtttaatt gaactccaac agtaagagga tttcgtttaa agtttaagtt ttcaggtttg
    41761 aaatcactaa tccaatcatc aatatgccaa cttgctataa gtgcaaaatt gctcaaccct
    41821 actcttaaaa ataaagaata ttcaaaagga gataagtatt ctggtttttt aaatttcaat
    41881 ttttggttta aaaactcatt atccattctg tacttcattc catatgaaaa tgcgtaagaa
    41941 tataatcctc ccaaatctat ataatatcct aagtttctgt aagttgctcc tgctaaatta
    42001 aatctaaaat aaattcctcc agaagcataa ttatgaaaac ttgaaatttt atcataaaac
    42061 atagatgggt caaaagttgg caaatcatct tttttaattg aagaagttct tttttgaaaa
    42121 gctatttctg ctccaatatc gaaaggttta gctattttga aacgataagt atatcctgca
    42181 gaaaaaatat tagagcaaag tgatttttct atgttactat tattaatagg tataggttta
    42241 gaataattta cataaaaata tccaaaatgc ttatcggcac atgaccattt agtgtcggag
    42301 ccatataaat ctttgtcttt aacagtttct gaatttcttt gagaaaaagc agttaaactt
    42361 attaaagcaa ataaaattag aattgatttt ttcattttgt ttttttcaca aagataatta
    42421 aaaagcagaa aaatagcctt taaatttatc gaaaatactt tcagtttttt tgtcttttgg
    42481 attaaagcta tcactttctt tcattttttg aagaagagct ttatcttctt tatttataga
    42541 agaaggtata aaaacattaa ttcttactaa taaatctcct tttgaataag catttaagtc
    42601 tggtaaacct tttcctctaa gtctgagtat tttgccagat tgagttcctg gctcaacttt
    42661 aattcttact tttccgctaa gagttggaat ttctgcattt gttccagtaa ctgcatctgg
    42721 gatactaatg aataggttat atattagatt gttaccgtct ctttgaaact cgggatgttc
    42781 gatttcttgt attaaaacaa ttaaatctcc atttatgcct ccgcgtcttg ctgcattgcc
    42841 ttttccagag acagaaagtt gtacgccctc acttactcca gcaggaattt ttaattcaat
    42901 taattcttca cctgttacta caccttcgcc atagcaagca gtacattttt cttgaataac
    42961 tttgccgtct cctccgcaag tttggcaggt agtagatgtt tgcatttgtc ccataatggt
    43021 atttactacg cgagtttgca ctcctctacc attacaagtt ggacaagttt tgcttgagtt
    43081 tccatttttt tcgccgttgc caccacaggt tttacaggct acgtattttt ttactttaat
    43141 tttttttgtt gtgccatttg cgatttcgtc gagggttaat tttacagtta ctctaaggtt
    43201 agttccccta ttaactctgg tgcgacttcc gcttccgcca ccacctccaa agaaagagcc
    43261 aaatccacca ccaaaggcat ctccaaaaat atcaccaaat cttgaaaaaa tatcgtcaaa
    43321 ggtcattcct tcgccactaa aaccaccagc accacccatt ccagcatgac caaacctgtc
    43381 gtatcttgct tttttgtcgg ggttagataa aacttcgtaa gcttctgctg cttctttaaa
    43441 cttttcttcg gcttctttat catttggatt tttgtcagga tgatattgaa tagccttttt
    43501 gcggtaagct ttttttattt cttccgcaga agcatctttg tttacatcta atatttcgta
    43561 ataatctctt tttgccatat tttattctcc gacaacaact ttagaatatc taattactct
    43621 gtcgtttagt ttataacctt tttgtacaac atctacaact tttccttttt tctcttcgtc
    43681 ttcaactggg acgcgagtaa cagcctcgtg aaagtctaaa tcaaattcta aatcttgagc
    43741 agctatttct tcaatacctt gtgcttttag aaaatctaaa aatttttgat gtattaattt
    43801 aactccttca accgtagatt caatatcttt tacattgtct aagtgttgca tagccctatc
    43861 catatcgtca acaacttgta taaaatcaag cattagcgat gctttagctg tttcgcgtat
    43921 ttcaagtttt tctttagcag ttctttttct aaaattttcg tattctgcaa caattctaag
    43981 atatttatct tttaattcat cgttttgttg ttgcaaaaga gaaatttctt ctttaagttt
    44041 tctttctttc cttcttcctg aagatttgta agtcttcttt tcttctactt gctcattttg
    44101 ttcattatta attgaatcgc atgtatcttc agaattttca atctctatgt ctttcgcttc
    44161 tttgttttct aaatcttgcg aattgttaat attttcgttc atgtttaagt tttaatattt
    44221 tttaattgcg gtattataat cacaaacagt ttgccacaat aatattacga caatttgtca
    44281 tgtaaaatag tttttgctgt cagattattt ttttagagtt ttaatagatt tttcaatatt
    44341 atctatattt ttctttaatt ttttcaactc cttagaattt ccgtactcat ctgaatgttc
    44401 taaattatta tattcgagtt ttaagtcttt tagtttatct tcaaaagata taactgggtc
    44461 tataattaca tatttttcta aagtttcttc tagaatactg cctctaaggt ttttagcaac
    44521 aataattccg tctttatcta ttaaaaaatt actgggaatt cctttaactc catattttcc
    44581 tgcaactgga gattgccagt atgccaaatc gcttacatta tgccaattta gtttatcttc
    44641 tttaattcct ttttcccaat tttctttatt tttatcaagc gatactccgt aaatagtaaa
    44701 tccttttccg attttgaagt ttttatcatg gtatttttcg tgtgcttgca ctacatgtgg
    44761 attttctttt ctgcatggtc cacaccatga agcccaaaaa tcaattaaaa caacttgtcc
    44821 tcgcaacgaa tataacgaaa tgctgtcgcc tttgggattt ggaagtttta tgtctggagc
    44881 aatgtcgccg atatttatgc caacttgttg agaaaatatg aaattagaaa agtgggctat
    44941 taaaattaga attaaaaatt ttttcataca ataaaatttt agtaattaat tctttcgatt
    45001 ttagcaccta attgatttaa tctttgatct atattttcgt agcctctatc aatttgttca
    45061 atattatgaa taatgctagt tccttcagcc gacattgctg ctattagcaa agccatacca
    45121 gccctaatat caggcgattg cattgtagta gctctaagtt ttattgctct atctaaacct
    45181 ataacagtag cacgatgtgg gtcgcataaa ataatttgag cacccatatc aataagtttg
    45241 tccacaaaga ataaacgact ttcgaacatt ttttgatgaa ttaaaactgc tcctttggct
    45301 tgagttgctg ttacaagaaa tacgcttagc aagtcgggag taagtccggg ccaaatagca
    45361 tcggcaatag tcattataga accgtcaata aagctttcga tttcataatt ttcttgagca
    45421 ggaataaaaa tgtcatcgtt aatgcgttca agtttaatcc ctaattttct aaacgaattt
    45481 ggaattattc ctagcatatc gtaatttaca tttttaatag taatttcaga gcttgtcata
    45541 gcagccatgc cgataaaact gccaatttca atcatatctg gcaaaatttt atgttctgca
    45601 ccgccaagtt tttctactcc tgtaattctt aaaagatttg agccaactcc agagatttta
    45661 gctcccatat taattaacaa tttgcataat tgttgtatat atggttcgca agctgcattg
    45721 taaattgtag tttcgccttt agctaaaaca gcagccatta tgatattggc agttcctgta
    45781 accgaagcct cttctaagtg tatataagta ccttttaatt cttttccgct aagtgtataa
    45841 aaattgtctt tagggtcgaa aataaattct gctccaagtt tttgaaaacc taaaaagtga
    45901 gtatctaatc gtcttctacc aattttatcg ccaccaggtt taggtaagca cacagaacca
    45961 aatcttgcta gtagtggacc taaaaccatt accgaacctc ttaatgcaga accgtcagct
    46021 cgatattcat cagattgtaa atattctaaa ttaatatctt tggcttgaaa agtgtaaaca
    46081 ccttttccat tttttgtaac ttctactcca agcgagccta atagttcgat aagtttattt
    46141 acatctctta tgtctggtat attactaatt ttaacctttt cttcggttag taaaaccgca
    46201 cataaaactt gtagagcttc gtttttagct ccctgaggat aaacatcacc ttttagttta
    46261 taaccacctg taactttaaa agtactcata ttaaattatt tttttcttct tgaacttctt
    46321 tttttagaac tagctttaga tgcttgtttt gggttgttgg gtttattttg tactaatact
    46381 tctctaaaat cgtttaactt taaaccttca ggaattccca caccaccttt agttaaaatt
    46441 tctaattctt taaatataat atcgtctgag atattttcct ttttccatgt catatacgag
    46501 cgtttcattt gatttgccat ttgctcaatc aaaacttctt ttttttcagc ttcttctata
    46561 ttcgaaacgt catttaagat tctctgtatt atttgtccgt aatgtctata ttttattttt
    46621 ccttcagaat aaggaatttt ttctggtttt tcctttaata attttatgtc tggcaaaggg
    46681 tagggccagt ctatatcaag tttaaaatct gacattatag ctagatgatc ccaaagttta
    46741 tgtctgaagt cggaaacgtc tttaaattga ggatacatgt tccccataat tgcaactaaa
    46801 gtgtgagcag ctttagttct ttcttctcta ttttcaatgg tgcaagcata atcaatcatt
    46861 tgttgaatag tgcgtccata ttcagagatt tttagctgtg gtctttgagt attataatcc
    46921 atataaaata atttttacaa aaataggatt tattatgtaa atcacaaaat taggtttata
    46981 aaattaataa ttttgcaaat tttaataata aggtttttgt gtcgcctgtg tcaaatttaa
    47041 ttattagttt tgaattacta ccttcgtcaa tgatttctaa tatttcacct tttccaaatg
    47101 aattatgtgc cactctcatt ccaactttat atgctgaaat gtctattgtg cctgtgtttg
    47161 ttttacttgc tttgttaaca ctaacgaaat ttccttttgg aacttgactt aactgttttt
    47221 gtggtttgtt ttcaaatgtt ttatattgag gcttgctttc aaaaatttca tcaaaaccag
    47281 ttctttcgtt agtgtaactt ggtgtttgac tttcgtcttt gccttcccat tctacaaaat
    47341 cgctatttat ttctctaata aatctgcttg gtctcatgga ttgtacgtta ccaaacttaa
    47401 acctattagt tgcataaaaa ataaataaat tttctttagc tcttgttatt gcaacataaa
    47461 acaaacgtct ttcttcttca agctcaacag tagattgaat actcattgca cttggaaata
    47521 aattttcttc ggcaccaact attagcacat ttttaaattc caaaccttta gcaaaatgca
    47581 cggtcattaa actaacctta tcattttcat tattatcctc ttcttcattg ttttctaaat
    47641 ctgaaagtag agagatgttt tctaagtaat caataataga gtctgattct gcaagagctt
    47701 ttctttggtc gcaaaaattt tgagcagcat taaataattc ttgtaagttt tcgtacttgc
    47761 taattccttc tggggttttg tcgctaaata gttcatggtt tatgcctgac ttatttacta
    47821 cttctaccaa aaattctgaa gcgttactag tttctatttg tttggcaaag ctatcaatta
    47881 agttagcaaa acttttaagt ttttgttgtg tcccaacatt taatccaaca ttgtagtttt
    47941 cgggataatt tataatttcc caaataggaa tatttgaatc ttcagaaaat tggaaaattt
    48001 tatttacagt tgttgctcca attttgcgag atggatagtt tactattctt ctaaaagctt
    48061 cagaatcttt ttggttaaca gaaaatctaa tatatgccac aacatcttta atttctttac
    48121 gttgaaaaaa cgatagcccg ccatgtattt tatgtggaat attgaatttt ctaagagctt
    48181 cttccattat tcgcgattga gcatttgttc tatataaaac agcacaatca gaagcagtat
    48241 aattgttatc agctaaaagt ttagaaatgg tttcagcaac tgcaattcct tctaaaccat
    48301 ctgttattgt ttcttcgatt tttattttac taccaatggt attttctgaa aataattttt
    48361 tctgaatttg atttttattc ttattaatta atgaattggc agcattaaca atattttgag
    48421 tgcttctgta attttgctca agtttatgta gttgataatc aggataatcg tttttgaagt
    48481 ttagaatatt ttgaattttt gcacctctaa atgaatatat actttgagca tcatcaccaa
    48541 caacacaaag tttacgatga gttgacgata atttttttat aattaaatat tgagaatagt
    48601 ttgtgtcttg atactcatca accaaaatat aatcaaatct ttgacagtag tgagctaatg
    48661 tttctggact aattttaaat aaaatatttg tatataaaag caaatcatca aaatccatca
    48721 cattagaagc tttgcagcgt tgtgcatatt ttgtgtatat agcatgaaaa tgctctctgt
    48781 tctctttgta atcttgaatt aaaaaatcat tgttttgagc atagccttct gctgtccata
    48841 attcgttttt tgcagcagaa atacgtgcag caacagcttt tggtttatac gtgtcatcgc
    48901 taaggtttag ttctttaata atagttttta tcaaacttaa tgaatctgca gcatcaaaaa
    48961 ttgtataatt actatcaaaa ccaactttat tggcttcgat tctcaaaatt cttccaaaaa
    49021 tagaatgaaa agtacccatc caaatatttt gagaagcttg atatcctaag agcttatcaa
    49081 ttctatcttt catttcgttt gaggctttat ttgtaaaagt taaagccata attctatgtg
    49141 gcttaatacc ttgtgaaatt agataggcta ttttatatgt taaaactcta gtctttccag
    49201 atccagcacc agcaaccact aattgaggtc cttcgcagta ttttacagca gttttctggg
    49261 tagcgtctaa atctttcaat aaataatttt ccacaaatct tttttttgac aaaaatataa
    49321 tatgaaaaaa ctgttatcaa ctatttttat aagtatttat taacgaaata atgaaaaata
    49381 cttaaaaaag atttttttaa tgtttttaga aaaataatta attagtttat taaaaatttt
    49441 tatcaacaca tatctatata aaatatggct ttagaaacat ggatattgtg cttatatata
    49501 atttagtgta aggaattgtt aataaaataa caggataaaa atatttttta tacatttgta
    49561 tttaagaaaa atgaaaaaaa aattaaaact catgcaacgt ttttgatgtt tcaactgtct
    49621 ataaggagag tttattttgt taaattttaa aattttaaaa tatgaaaact acaaaaatta
    49681 tttttttact tgctttcatt tttggttctt tcatctgtaa tgcacaggaa gtaaaccaaa
    49741 aattgttagt taaatttgat gcaaacgaac tcaaagaaat gaagaaaacc aatgttgaaa
    49801 atcttaattt cttagagtat tatgttagcg atgctttgta ttttgtacca gttccagaca
    49861 aacctgtcaa ttataaagaa ttggttagaa ttaacccaaa gacaaaagcg atttctgaaa
    49921 atcagactat tacacaagct gatatagatg attttaatcc tttggagtat gatattagtt
    49981 acgaacctga tagatatact tattaccttg ctggtaatac aggaatgctt ttagttgtgc
    50041 cttcacaaag agatttaaaa ttaggttctg ataatagagt aaaatctcta aaattacaac
    50101 aaacacaaca attagataaa attaataaaa aataagatac tatgaaaaag ttacttagtt
    50161 taatattgag tgtatttgtc ttatccctat atggatatgg acaatacgat atggttacta
    50221 acaatggaca aactatatct gcttgttctg gtagtttgag cctaggttct tatactgtgg
    50281 gtcaaacata cactttaact atatgttcaa atgatcctgt aaataagcat ataacatttt
    50341 cagtaactgg aggttatact tttcctgcag gaacagaggt gtgtttttat gacggtgcgt
    50401 caacatctgc tccattaatt ttatgctggg acagtggaac aaccagtggt cagttagcgg
    50461 tgcaaactgg aggcggaaac gaaagtggtt gtttgactgt gaggtttact ggaggtgctg
    50521 ccggagctag tttaggaaca aataatataa gttgtaactt tgtttgtcaa ccacgtgagg
    50581 ttgttattgt ttcttccgat cctccattag ttggtggtac atatattgat gtatgttggg
    50641 atgaagctaa taaccaaact tttcctataa cttttaatgc acagggaact tatccagcaa
    50701 caggttatga atgtactgat gctacaaata cttttacttg gaactttaac gatggttctc
    50761 ctttagttac tggaattgga atgtcaacag ttactcacac tttccctgaa cgtagaggat
    50821 atactgtaac tgtaaatatt gaagattctg aaggttgtgt gaatacaaat gcagaacaaa
    50881 aaagagtaag ggtttcttta ccgccaatat ggaataactc aacaacagtt ttaacaacta
    50941 caccgccaac tacgccacct gcaatttgta tgggttcgcc tgtaaatgct tgtgcttatt
    51001 ggtcaaatcc acaatggact agtgttattg ctcttgcaag tggagatacc gtttgtgtac
    51061 ctgatacacc accactttgt tatgagtcaa cattgatcga aacttcattt gagccaggac
    51121 aaatacttac ctcaataaat gatattatat ctattgatat gaatttagct catgactatt
    51181 taggagattt aacattttat attatttgtc ctaataatca aacagttatg attgggaatc
    51241 aaggtggagg ctctgcaaac ttaggcgttc caggaccaga tagaaacagt tgcgagggac
    51301 aacaaggttg gatgtatcac attacaccaa cagcaactca aacaatgcaa caagcaggaa
    51361 gtggtatttg gagtggtagt ttaccttctg gcaactacgc ttcttatcag tctctaactg
    51421 ggttagtagg ttgtccttta aatgggcagt ggaagttaaa aatatgcgac aactggaacc
    51481 ttgatgctgg aacaatattt ggctggtcta ttcaattcca agaaagttta tatccagaag
    51541 tttgggacta ctctcaaaca tatacaccaa caatttggga tggactttac ggttcggtag
    51601 ttaacgatcc aaataatcaa aactgtatga caggaactta tattacatct gcaacaccag
    51661 acgtaaactc actacaacct tttacaatta caataacaga caactttggc tgtactcacg
    51721 atacttcttt atatgtaaca gttcgtcatc aacttgacga agaatgttgt caaattccaa
    51781 atgttaacgc aggtgctgat gtagcagttt gtgctttaac ttatgactta tctgctagcc
    51841 cattggctta tccatcaaat acaggaaatt gggaactaat ttcaggtcct ggcactgcaa
    51901 catttactaa tgtaacaagc ccaactacta cagtgaacgt atcagtttat ggaacttatg
    51961 tgtttagatt tcacgaaagg tataatgata atcctggttg tgctagttct gacgacgtta
    52021 ctattacatt taatcccact tataatccaa cactttctcc agttgcaaat atgtgtgtaa
    52081 atgcacctcc ttttatgttg gaaatggttg attttggaac tttaagcgtt aatataaatc
    52141 ctgaattatt aaatgtaaca acaggacaat ttaatccttc aaaaccaggt aattatacta
    52201 taactaatac tgtacaagat ccatgttcag gaggttcgct aactcatagt attacgttta
    52261 ctgttcatga tgctattact gttcaaaatt tcacagaata ttgtggacca acaggtagtc
    52321 ctacatactt ttatgtagaa tttgatgttg tcggttctca aggaaaccca tttacaggtt
    52381 attatgtaaa tggaacattg caaaataacc cacattatac tgcaactata caatctccag
    52441 gttcttatgg atatactgtt acagatgcct ataactgtag tagttatcct ataaatggat
    52501 ttagagattg tggttgtcca ttttacgcag gtactatggc ttccttacaa acagtaatac
    52561 tttgtcaaac cgaatgttcg ggagatgaag tttcacataa taataaccaa gaaacagatg
    52621 gtggagctgg agtttttgaa tttatgatac ataatggtaa taatcaacca attgcatata
    52681 gtaacactcc aaacttctgt cgaaattcaa caacaccacc tttgtcgttt aatactcttt
    52741 attatatatc tccaatttgt ggtttaccta ctggacctgg tggacatgct aatataactc
    52801 ctccaaattg ttattctata ggacaaggta caccagtaat gtggttgcaa gatccagtgg
    52861 ctcatgtagg accaagtaaa gatacttgtg gattaattat tcagttacaa ggtaatgctc
    52921 caacagatgg tatgtatggt tactggtctt caacttgtga ttttattact attaatggaa
    52981 caagccatac agatccaaat gcagtagtta tggtttctga ctatgaagac tgtacattta
    53041 cttggaatat tgtaaatgga gaatgtgttg ctaatgccga tgttttaatg agatttttag
    53101 caactcccgc accttatgcc ggtcctgatc ttgtagtttg tggaaattct gcagaaatgg
    53161 caggggtagc aagtatgggt ggaaacttaa cttggtcggg aagtggaaca atatttaatc
    53221 ctcaaacatc agcagatgct acttctaata ttagtgtttt tggaacatac caatatacac
    53281 tgatagaaga taatggtact tgtactggtt cagataaagt tttagtaact tacataaaag
    53341 aaccttcgcc aattacaaca ccaaatgttg atactgcttg cggagttact tataatctta
    53401 aagtaactac tgttccaaat gctgttgggc aatggacatc ttatgcgtgg aatgaggcta
    53461 caagcgaatg ggagcttgct tatccatata tgacaccaaa ttctcaatct actgacgttc
    53521 aagtacagat tggcaatttt agtggtatta ctcgtcgttt ccgctttgtt tggactgaaa
    53581 caaatactac aggaggcata gtttgtcagg gaacagcatc taaagaggtg gtttttgcaa
    53641 aaataccaca ggctagcgtt gtacaagacc aacttgaagt atgtggtagt tgtgttgact
    53701 tacatgctaa tgtaacaagt gaaacttata attatgatta ttattggata ccaaaaatag
    53761 atggaaattg gacaaatcca gaaggacgtt tttctcctga tgcaagtttc tgtgttgaag
    53821 gtctagcagt ttttggagat tctgcaagcg ttaatgctga gttttactgg attgttagaa
    53881 atggtactag ttgtgtgtca actgctgcag tgaatgttac tttccatcaa caaccacaag
    53941 caaatgcagg tttagataca attatttgtg gacttgaagc taacctccat gcttattgga
    54001 gttttccacc aaatccaagc tattctccta gcggagtttg gtcaacagtt ccaaaaccag
    54061 gagcaacagc aactatacaa gataaatata atcctatttc gcctgtaact gtgagcgaag
    54121 ctggagtttg gaacttcgtc tttagagaaa ataatgaaaa tttaggtagt tgttactcta
    54181 ccgatactgt tcaaattgag tttgtagaaa ctcctatacc ttttgctgga gaagataaac
    54241 atgtttgcgg aaaatgtgta actctagaag ctacatctgg tggattttct ggtacttggt
    54301 tgccaaatgg agcatctttt gttgattatg aagacccaca tactaacgct tgcgtagctg
    54361 cttatgatac agtagtgttt atatggcaag agtcaaatcc ttctataaca gatcctgcat
    54421 tcgcctgtgt tgctacagat gaagtaaata taattttctg gcgagtgcca acagctaata
    54481 ttataacagc tgtagcagat tctgcagttt gtggtttaac tttctatgaa ttaagagcgg
    54541 aattaccagg tactggaata acaggctatt ggtattgtca taattcagga cttgaatatg
    54601 gagataaatt ctcaaacaat acctgggtaa aagttccaca ttatggatat tatgattttt
    54661 attggataga acaaacag
//
LOCUS       DAOPNX010000005        52488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_826_length_52488_cov_6.366449, whole genome shotgun sequence.
ACCESSION   DAOPNX010000005 DAOPNX010000000
VERSION     DAOPNX010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 52488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52488
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_826_length_52488_cov_6.366449"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..1129)
                     /gene="mltG"
                     /locus_tag="PKN53_01035"
     CDS             complement(71..1129)
                     /gene="mltG"
                     /locus_tag="PKN53_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOD87570.1"
                     /translation="MGKAKKKNRKRIIIIILVVFLIIAAIAGVFAKKFYDYAFAPNVK
                     LEEKTAYLYIPTNSNYEDVLQILEDTEYLEDMESFKLVAEKKNYPSKVFAGRYEIRNG
                     MSNNDLVNLLRSGKQTPVKVSFISVRSLDILAGKVAANIEADSTEIAQLLKSPETAQK
                     YGFDERTFMAMFIPNSYEFYWNTSAEQFVERMAKEFKAFWTEERLQKAENIGMTQSQV
                     STLASIVEVETQQNDEKARVAGVYINRLNLGMLLQADPTVVYAVGDFTLKRVLNKHLE
                     IESPYNTYKYSGLPPGPINIPSISSIDAVLNYEKHNYKFFCAKDDFSGYHVFAVTNAQ
                     HEANARKYHKALRNAGIR"
     gene            complement(1168..2403)
                     /locus_tag="PKN53_01040"
     CDS             complement(1168..2403)
                     /locus_tag="PKN53_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOD87571.1"
                     /translation="MKKTCFTISLIFIIFVCCTNRLRNEELVKTIVTEKQEDNPKILP
                     GIYQTQEYLPLLSDKKVAIVSNQTGIINNVHLADTLLSSGVDVVKVFCPEHGFRGTAD
                     AGAKVDNTIDEKTGLPIISLYGKNKKPLPEQMQDFDILVFDLQDVGVRFYTYISTLHY
                     VMEACAEANIPVIVLDRPNPNAHYVDGWILDTANYRSFIGMHPVPVVYGMTIGEYGQM
                     INGENWLGNGVKCDLTIIPCKNYTHNSKYSLPVKPSPNLPNDRAIELYPSLCFFEATN
                     LSVGRGTNKQFQIIGHPLLENVEQANFEFTPMPNEGASNPVLNGKKCFGFDLSDSNSI
                     FEIKENQLNLDLTLKTYELFPEKSEFFKKSNSLELLSGSGEFRKQVESGVSEKEIRES
                     WQDDLENFKRIRDKYLIYE"
     gene            2616..4679
                     /locus_tag="PKN53_01045"
     CDS             2616..4679
                     /locus_tag="PKN53_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOD87572.1"
                     /translation="MDFALTYPETKVTDVKDNYFGTEVADPYRWLEDDNSEETAEWVK
                     AQNEVSFKYLESIPYREKIRDRLVKIWDYPKVSAPTEKDGLYFYFRNDGLQNQSVLYY
                     KKGLESEEIELLDPNNFDKEGTVSLSNVCISEDCKYLVYAISRAGSDWREIYVRDIET
                     LKDLDDHLEWVKFSSIAWLNDGFYYTKYPEPEKGQELSGINTNSKIYYHKVGTDQSED
                     ILTYEDPKNPEIGFSAYVTDDEKYLIIHATKSTSGNSLYYKDLEKPNSTFVKLVDDFD
                     NDYTVIHSKDNKLYVYTNYQAPMYKMIEIDLTKPDRKFWKDFIAEDKSVLETVNYIGE
                     RFIANYMVDAHSVVKIFDEQGKFLHDLDNKEIGTIYGFSGDKDSDVTFYTFTSFTTPA
                     VIYSYDIKANKSSVYEKANINFNTDDYETKQIFYTSKDGTKVPMFIVHKKGIALDGKN
                     PTLLYGYGGFNISLTPSFSISRCVWLEQGGIVAIPNIRGGGEYGEEWHKAGTLMRKQN
                     VFDDFIAAAEYLISNQYTSPERLAIQGGSNGGLLVGAVANQRPDLFAVALPAVGVMDM
                     LRYHKFTIGRYWATDYGTSEDSKEMFEYLYKYSPLHNIKPDVEYPAVLVTTGDHDDRV
                     VPAHSFKYIATLQATYKGERPVLIRIETQAGHGAGKPTKKIIDEIADTYAFTFYNMGF
                     TPVFQ"
     gene            4805..5347
                     /locus_tag="PKN53_01050"
     CDS             4805..5347
                     /locus_tag="PKN53_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87573.1"
                     /translation="MKKISIIILATLGFSMIFSACKKYEEGPALTLRTPKQRVVGTWN
                     LTETRYNNVKIDFNNLQDFFNSFDLGGLDLSNAPDSTTFDLSQLSLKSIKLNLDKEGN
                     GAISIELAYGMLQFPYSQEITWSFDDKKDALIFKIKDVAEEQSFKILKLTKDELWLEK
                     TDVNNGVASIVNFEFEKIKD"
     gene            5413..>6320
                     /locus_tag="PKN53_01055"
     CDS             5413..>6320
                     /locus_tag="PKN53_01055"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD87574.1"
                     /translation="MINKLYSLFPPFLKRFAIFFHRTLRLFYFNLTKKKSYKNCSSIS
                     QKVFEEFNKSRPYGPLKKICYAPFSSMFFSRHGLVAPCYATYNENSSHISNNSIKDIW
                     FSGSINEIRKQHSRCDLSTSCSFCENILNSNSYKSALINKYEHYAFSKSKYPKIMEFE
                     LSNKCNLACIMCDTNLSSSIEKGKNQNFTGNLFYKESFFEELREFIPHLQLAEFTGGD
                     PFMIEEYYKIWDIIMELNPKCRILITTNANTMNPRIERLMNMHKNLYFNISIDSLQKE
                     NYEKIRINGNFEYAMKNTQKFIDYCK"
     assembly_gap    6321..6420
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6421..6729
                     /locus_tag="PKN53_01060"
     CDS             <6421..6729
                     /locus_tag="PKN53_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87575.1"
                     /translation="NFENLIELLKSWSKENELDIKHDNIEEKTLSETEIINLLKLKVN
                     ETKPEFFKKFDNFSQEVLQLPNKDIILKKIYNFSDEIFFEYLENKNIDELIEICKTIK
                     "
     gene            6726..8165
                     /locus_tag="PKN53_01065"
     CDS             6726..8165
                     /locus_tag="PKN53_01065"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD87576.1"
                     /translation="MKNKIHNIEFFARTKIKKFYNLIKYFPISKHINSNIENKKNLVK
                     YNYSRSIGVEPCVCHAPLRSIYFDMKGNATACCFNRTHILGKYPEQSIKEIVFSEKRD
                     FLQKELCKQNFMYGCKHCLNLIEAQNFEGVEARLYDNLKAQGKMPSEMIFELDNTCNL
                     ACEMCHEGFSSTIAKLKNIKVPPPPYDSKFLEQLKEFIPHLEVAKFLGGEPFLINIYY
                     QIWDLILELNPKCRIHLQTNGTIFNEKIKSYLEKGNFYIGVSIDSLDPENYKKIRQNS
                     NLEKVISNLFKFAEISKEKHTFINISVCPMLQNWKEVPQIVDFCNQNSFFVYFNTVYT
                     PGFAISDADEDLLLEIINYYKNFTFKTKGIIAKRNLKFFNNLLTQIKAIYKPKAEASE
                     YERKRHKWTSNMLKNFMLKKVNNDKELSSKIEELFTNTNNEFYFSDKDLENLEKLKIE
                     DLITALKNETLIELQERIKRFINFGRFSK"
     gene            8253..9419
                     /locus_tag="PKN53_01070"
     CDS             8253..9419
                     /locus_tag="PKN53_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87577.1"
                     /translation="MKQVWILVFLFSFVILSCNNKKKEAQVADNDTETIEAPIHVYDT
                     FSYKGFNEIANYIGGTQPEEEAIYFSKYFSDPDWKNYANANSESWNLHSQQVLDIVSD
                     WSEKNLTYGDTVFYPFGGPDFNYLDAMFPKAKHFVMMGLENVGDIPDLEDIEKYGTKS
                     YLSNIQQAIQHNLNLSFFQTNSMKVVLNSDIINGTIPIIMYFLNRHDKVIVNINPASI
                     NEQGDISVENPNEEFAYNYKKKFNNGVEFVYREKDTKELKKLTYLSLDICDIMFTKPH
                     ELQFITNYTKNNTVYLKAASYLCFRDTFSKIRNIILDNASQVVTDPSGIPYKYLKSKF
                     DVKLYGEYNGPIKLFAERNQPDLKQAIDSIKPGRLPFKYGYHPTHWCIMVATKR"
     gene            complement(9442..10833)
                     /gene="lpdA"
                     /locus_tag="PKN53_01075"
     CDS             complement(9442..10833)
                     /gene="lpdA"
                     /locus_tag="PKN53_01075"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOD87578.1"
                     /translation="MNFDLIVIGSGPGGYVAAIRGAQLGLSVAVVEKSELGGVCLNWG
                     CIPTKALLKSAQVMNYVKNSEAYGIINDGTAKPNFAKVISRSRDVSANMSKGIEFLFK
                     KNKITVIRGYGKLKSASEVVVTDSENNSETYSAKHIILATGARSREIPGIAIDRKNII
                     TYREALCLEKLPQSMVVVGSGAIGSELAFFYSSMGAEVTLIEFMPEIVPLEDEEVSKQ
                     LARSFKKAGIKVMTGTAVKNVDVVDGRCIVNYTDKKGENTIECEVVLSAVGVTANIEN
                     LGLEELGIKTEKGKILVDEYYRTNVDGVYAIGDIVPGHALAHVASAEGICCVENIIGL
                     NPPVVDYSNIPSCVYTTPEVASVGFTEQKLKEMGKAYKVGKFQFIASGKAVASGFRDG
                     FVKLLFDEETDELLGAHFVGDNIAEMISEMVVLRKLKGTAKDLINSIHPHPSLSEAIM
                     EAAAAAHGEAVHL"
     gene            10981..11781
                     /locus_tag="PKN53_01080"
     CDS             10981..11781
                     /locus_tag="PKN53_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOD87579.1"
                     /translation="MERNILVPYEFSNESNYALDHAYELAKTSNYPIHMLYVASNHED
                     ADEWLIELEKVAKKFSEEHNNHKTVATVRVGNLFKTINDYGEEINAYFAVMGTHGLQT
                     IDKAMKLIKKFDRMPSILIQKPMHYGEYDKICVPIDSDKKSRAKFLWVKYLSNIFESK
                     VYVVHPDFKNSLRKQNLNSNLNFAGTIFERENIDFEVKVLPEENYADHLYDYISEIEA
                     DIVLFMTDKYKRIITNVQRPRNIELSKKIPIMCVNIRTDIQKLGGFTY"
     gene            11919..14624
                     /gene="ppdK"
                     /locus_tag="PKN53_01085"
     CDS             11919..14624
                     /gene="ppdK"
                     /locus_tag="PKN53_01085"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOD87580.1"
                     /translation="MTKIKRVYSFGNKKAEGDAKMKELLGGKGANLAEMALMGLPVPA
                     GFTITTDTCTEYYNNNHELPEGLMDEVWAAMKKVEADMGLKYDDPDNALLVSCRSGAR
                     SSMPGMMDTVLNIGLSSNTIPGLIKKTNNPRFVYDSYRRLIMMYADVVMDKAEDLDKN
                     IRRQLDDMFEEYKVQRNYKSDTELTAEDLMIISDLFKKKIKAVLGTEFPDDAKKQLEG
                     SIKAVFKSWNGKKAVSYRRIEHIPDDWGTAVNVQAMVFGNMGETSATGVAFTRNPATG
                     ENLFYGEWLVNAQGEDVVAGIRTPNPLNNDTKNSQNEHLMSMQEQFPAIYKELDDIRT
                     TLEDTFKDMLDIEFTVQEGKLYMLQCRVGKRTGTAALNMAMEMLEEGRIDEKTAILRV
                     EPSQLDELLHPIVDPAAEKNHEPIVKGLPAGPGAATGKVVFTAEDAVEWTKRGEKVLL
                     VRKETNPEDVEGMRVADGLLTTLGGMTSHAALVARGWGKCCIVGAGNLEVREDEKIAI
                     VKGTDIVIKEGDILTLNGTTGNAYNTVIKLMDSSENPLFQKFMTLVDKHRKMGVRTNC
                     ESPEDAKLAIEFGAEGIGLFRTEHMFYGKGADEPLFILRKLILASSKEERIAAVNELF
                     PFVKRDMKDTMAVMNGLPVTFRLLDPPLHEFAPHTKDKQAEIANAIGISAEEVAQRVD
                     ALSETNPMMGHRGVRLGITYPEITEMQFRALFEATAELMKEGKKVTPELMVPVTADVK
                     ELDITKVLFDKVKKEVEDKFGIKLSCAYGTMIEIPRACLLANDMAKTAEFFSFGTNDL
                     TQMTFGFSRDDVSGFMHDYLENQIIPVDPFQTIDQAGVGQLIEMAVVKGRATRNNLKI
                     GICGEQGGDPASVEFCYKMGLNYVSCSPYRVPIARLAAAHASIKF"
     gene            14636..15001
                     /locus_tag="PKN53_01090"
     CDS             14636..15001
                     /locus_tag="PKN53_01090"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HOD87581.1"
                     /translation="MELEGKLIVKLDPVTGEGRNGPWKKQSFVIETLDNYPKKVCFTI
                     WGDRVPLNNFTESDTIRVLFDAESREYNGNWYTDLKCWKIELSSPGNVGQAPMETPPF
                     SADDAPFELDSDPGDDLPF"
     gene            complement(15065..15487)
                     /locus_tag="PKN53_01095"
     CDS             complement(15065..15487)
                     /locus_tag="PKN53_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOD87582.1"
                     /translation="MSFNYEYPRALNTVDAIIISKKENKILLIKRLNEPFKNFWALPG
                     GFIEMEETLLESVKREVLEETSLDNINLREFKVYGDPGRDPRGRTITFVYYGYCDSPE
                     LAQAGDDAKELAWFSLNELPDLAFDHKKIIDDFLSLLI"
     gene            complement(15477..16142)
                     /locus_tag="PKN53_01100"
     CDS             complement(15477..16142)
                     /locus_tag="PKN53_01100"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD87583.1"
                     /translation="MKSADKILQRIKEGEGLNLDFKHSINDAKKIARSLSAFANTKGG
                     SLLIGVRDNGSIAGVNSEEEYFMVETAALIYCKPSIKFSVNNFNVLGKQVLEIIVSES
                     ETKPHTAPDNDGVFKAYVRVNDENIIAPKPLVEVWKRLQNNVKGVKINFDENISFLLK
                     EINQNGSISKSQFVRHTGIKSYVANKILTNLIIMNILDLEITASETRYVFNKNFDFNK
                     HEF"
     gene            complement(16270..17505)
                     /gene="rocD"
                     /locus_tag="PKN53_01105"
     CDS             complement(16270..17505)
                     /gene="rocD"
                     /locus_tag="PKN53_01105"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOD87584.1"
                     /translation="MSKDVKRTSQDFIDLEAKYGAHNYQPLPAVLDRGEGPFLWDVEG
                     KKYFDFLSAYSAVNQGHCHPKIINALIEQAKKLTLTSRAFYNSTLGDYEKFITEYFGY
                     DKVLPMNTGAEAVETALKLARRWGVVKKGIPNDDVKIICCENNFHGRTITIVTMSSDP
                     DSYANYGPLTPGFIRIPYNDADALENVLKEHGKTVAGFLLEPIQGEAGVFVPDDGYLA
                     KTKAICEKYNVLFIADEVQTGIARTGKLLACDHENVRPDIIILGKALSGGVIPVSAVL
                     ADDDIMLTIKPGEHGSTFGGFPLAGKVATAALEVVRDEKLAERAEYLGKIFRSEMKSI
                     KSDMIQLVRGKGMLNAVVINNKPGKTALDVCMQMKELGVLAKPTHGNIIRFAPPLIIT
                     EEQLREAIELVKEAFKKFE"
     gene            17719..19041
                     /gene="ffh"
                     /locus_tag="PKN53_01110"
     CDS             17719..19041
                     /gene="ffh"
                     /locus_tag="PKN53_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOD87585.1"
                     /translation="MFENLSERLERSFKLLKGEGKITEINIAETLKDVRRALLDADVN
                     YKTAKTFCDDVREQAIGQNVIKSLKPNQLMIKIVHDELAKLMGGKAVDINIKGNPAVI
                     LMSGLQGSGKTTFSGKLANMLKTKKGKNPLLVACDVYRPAAIEQIKVLGEQIDVKVYA
                     EEDNKNPVKIAQNSIKHAKMYGHDLVIIDTAGRLAIDEEMMDEIENIKKAVNPQEILF
                     VVDAMTGQDAVNTAKEFNDRLDFDGVILTKLDGDTRGGAALSIRSVVNKPIKFVGTGE
                     KMEAIDVFHPERMADRILGMGDIVSLVEKAQEQFDEEESRKLQKKIAKDQFDFEDFLA
                     QIKQIKKMGNIKDLASMIPGLGKAIKNLDIDDDAFKGIEAIIYSMTLEERRNPNIING
                     SRRKRIADGSGTTVVEVNRILKQFDETKKMMRALSPNKTKKKMARRMR"
     gene            19085..19960
                     /locus_tag="PKN53_01115"
     CDS             19085..19960
                     /locus_tag="PKN53_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HOD87586.1"
                     /translation="MELIDGNKVAKEIKEEIKIKALQIKQKCGRAPHLAAILVGNDPG
                     SESYVNYKMKDCEEVGFESSLIRLEENAAEEELYKIIDKLNSDPLIDGFIVQLPLPKQ
                     FDSQKVIELIDPQKDVDGFHPINVGKMTIDLDSFKSATPMGITILLNKYGIETSGKHC
                     VIIGRSNIVGRPLSILMSQKAMNSTVTVVHSKTKNIAEICRHADILMTAMGSPKFVTA
                     DMVKEGAVVIDVGTTMIESNKTKSGYKLTGDVDFENVAEKCSYITPVPGGVGPMTRVA
                     LLLNTLKAAEQNIKK"
     gene            20031..21122
                     /gene="hydE"
                     /locus_tag="PKN53_01120"
     CDS             20031..21122
                     /gene="hydE"
                     /locus_tag="PKN53_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOD87587.1"
                     /translation="MHNKIADILQKSVLSKNDLIILLSANKDNAEIIFNKAAEIKTKY
                     IGNYTYFRGLIELSNICTRNCLYCGIRSGNKNAERYMISDEEVFNSVKYALDRHWGSV
                     VIQSGERSDTAFIDKVEHILRQITQMCEKKPGITLSLGEQKTDTFKRWFDAGATRYLL
                     RIESSNPDIFKKIHPQDKLHNLERRIENLYTLQKLGYITGTGVMIGLPFQTIEDLAND
                     LLFMQKLDIDMIGMGPYIEHEDTPLYQYRDLLLPIEERYFLSLKMIAVLRILMKDVNI
                     ASSTALQAINPDGREEGIRVGANVIMPNITPVRYHKSYNLYKGKPDIAPETDNYIKIL
                     EKQIALAGDKVGYGDKGDPKHFFRRTVER"
     gene            complement(21214..21762)
                     /locus_tag="PKN53_01125"
     CDS             complement(21214..21762)
                     /locus_tag="PKN53_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87588.1"
                     /translation="MKTKNLFLLSVLIFVNSFTFSQNQNLYENTAEEKVLYDLIMAYR
                     KQFDLPRIPISRSLNFVAHTHAIDLKNNYKHNDRCNLHSWSNKGNWTACCYTPDHANA
                     DCVWSKPGELTNYKHYGFEIAHWNPSGPTASSALNAWKKSPAHKNMIINKENWSDNEW
                     KAIGIAIYEEYAVVWFGMEPDR"
     gene            complement(21860..24199)
                     /locus_tag="PKN53_01130"
     CDS             complement(21860..24199)
                     /locus_tag="PKN53_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HOD87589.1"
                     /translation="MHLYFDKPATCWEETLPLGNGRIGMMIDGAIDMEEVILNDITMW
                     SGSVDLETINPNAKEQLPLIRQLLLEGKNEQAQELVYHYFKCGGKGSNFGNGKDAPYG
                     SFQLLGKLKVKHFYPNKSEVTEYSRKLSLNDAVATTQFKKGKFTYKRQYFASHADDLL
                     VAHYTTDNKGELSMEIMLERPERVKIDVYADEIVMQGQLNDGYDGDKGVRYFFTLKIQ
                     KNGGMHSTTDTSLIISNASEVTLLMSTSTNMLDKNYIQTVQDNLSNAEKYSFEQLKKR
                     HIEVYREKFDRVKLDLGVQKLDVSTDQRLLQFQLDDDPSLVALYFQYGRYLMISGTRE
                     NSLPLNLQGLWTNQVQTPWNGDYHLNINLQMNYWLAEVGNLAELHLPLVRLTESMQAS
                     GEKTAQGFYNADGWVAHMMTNPWYFTAPGEHASWGATNTGGAWLCSHLWEHYQYGLDT
                     AFLRSVYPIIRGAAQFFLSSMIEEPQYHYLVTAPSSSPENGFKIPNSDKTIYVCHGPT
                     MDVQIIRELFNNLLSAAKILNINDNITEAVEQALLRLPPNRISPKGYLMEWLEDYEEV
                     DIHHRHVSHLYGLHPSNQISPDFTPELAQAAKVTLNRRGDAGTGWSRAWKINFWARLK
                     DGNRAYSLLKKLLEPAWNPENSEEQRAGTYPNLFCAHPPFQIDGNLGGSAGIAEMLIQ
                     SQHGFIELLPALPDKWKNGSIKGVCVRGAGEIDFVWENHQVKEFTLRAKADHQYKIKI
                     PDYVKTVKLNNKKIEGKDTPFLNLNLKKGDEVRVKLIPY"
     gene            complement(24641..25801)
                     /locus_tag="PKN53_01135"
     CDS             complement(24641..25801)
                     /locus_tag="PKN53_01135"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD87590.1"
                     /translation="MVVHREIVTAMRQLGDKFPVVVLTGPRQSGKTTLLKAMFYDYRY
                     VSLEDPDMRIFAESDPRGFLKQYDKYIIFDEIQRVPHLFSYIQSIVDDSGIMGQFIFS
                     GSQNFHLMQNITQSLAGRVAIFRLFPFDFKELKRAGLLQNDYTTHLLKGFYPAIYNRK
                     IKPADFYSNYIETYVQRDVSELLAIQDMSLFKKFLSLCATRAGQILNMSSLAKESGIS
                     DPTVKAWLSALENSYVVFLLQPYYKNFSKRITKSPKLYFYDTGLLCNLLRINNVSKIK
                     NQAVKGALFENMIVSEYVKKKYHQNARQTDLWFWRDSHGNEVDLLLEKPQETEIVEIK
                     ATQTVMPDLFKGLNYYAALETEIKLTKTLIYGGSERQERSVAQVVPWNEVSV"
     gene            complement(26243..27382)
                     /locus_tag="PKN53_01140"
     CDS             complement(26243..27382)
                     /locus_tag="PKN53_01140"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOD87591.1"
                     /translation="MLLRKFNFQDVETNSIFLWGARQTGKSTLLKMRFPGVRYIDLLK
                     SDEFARYSRRPALLREELSLLPKNELIIIDEIQKIPALLDEVHWLISNHHLRFILSGS
                     SARKLRRSGVNLLGGRAIRKHLYPFVSVEIPEFDIVKACNNGMLPPHYLVENATNRLQ
                     AYVGDYLQQEIKAEALTRNLNTFTRFLEVAALSNGEILNYNNISLECGVSAPTVKEYF
                     SILEETLIAYIIPAFTRNVKRRVIQSPKFYYFDVGIANFLLKRTSVNPGSPEFGHAFE
                     HFIMQEIIAYIGYFRPLQNLSYWRTSSGYEVDVIIGNAEVAIEIKSSDEVQSHHTKGL
                     KAFSEEFPNCRLIVVSLDKYARRLNNVEVYPALEFLSMLWNGVFF"
     gene            complement(27542..30172)
                     /gene="alaS"
                     /locus_tag="PKN53_01145"
     CDS             complement(27542..30172)
                     /gene="alaS"
                     /locus_tag="PKN53_01145"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOD87592.1"
                     /translation="MMDSKQIRQKFKDFFEEKSHKIVASDSMIIKNDPSLMFTNAGMN
                     QFKDIFLGSRKPESLRVANSQKCLRVSGKHNDLEDVGHDTYHHTMFEMLGNWSFGDYF
                     KKEAIAFAWELLTEVYKIDKSRLYASVFGGDEAENLEIDTEAEEIWKEYVPEDRIVRG
                     CKKDNFWEMGDMGPCGPCSEIHVDIRSDEERKKIDGKDLVNKDHPQVIEIWNLVFIQY
                     NRKADGSLEVLPQKHVDTGMGFERLCAVLQGVKSNYDIDIFQILINHISEISGKKYGE
                     DEKIDIALRVIADHLRAVAFAIADGQLPSNNKAGYVIRRILRRAVRYAYSFLDLKEAF
                     IYKLVDTLVDLMGDSFPELISQKSIIEKVIKEEETVFLRTLEKGIRLLDTEMERLKKE
                     NKNTVNGTQAFILYDTYGFPLDLTELIARENGFVVDVEGFDGEMQKQKERARAAAEID
                     TDDWTVLKNIEETEFVGYDNLKAEINIVKYRRVEQKNSKYYHLIFDKTPFYAEAGGQV
                     GDSGFIRSINENIEIINTVKEHNLVIHISKYLPQNPEATFTAEVNVENRTSTANNHTA
                     THLLHHALRGVLGSHVEQKGSLVSPERLRFDFAHFNKLSDEEIRLIERQVNKNIRQNI
                     SKNEKRNIPIKKAKEMGAVALFGEKYGDEVRVIQYGDSVELCGGTHVESTGQIGFFKI
                     VSESAIAAGIRRVEAVTGEVAENLVFEKFDTVSDIALIFNNQKDLLKAINNVLEENEK
                     LKKQFEKFHAEELKVVKRQLLEQAENVGDVKLIVSEIDLKDANDLKEICSQIRGEVSQ
                     FACVLATAIEDKPSLIIAFSENLTKEKSLNAGSLIRDAAKFIQGGGGGQAFVATAGGK
                     NPNGISQALEFAKNEILKVI"
     gene            30278..31255
                     /locus_tag="PKN53_01150"
     CDS             30278..31255
                     /locus_tag="PKN53_01150"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOD87593.1"
                     /translation="MTKIKTKYKFNSETLQYEKIKFSFKKFFLKLFQRSVVSFSFGAI
                     LMVLYIIIYESPEEKLLRAEVKFLQENLNKINQRLKVNDSLLNVIAKNDNYIYRTTFE
                     LDTIPTSVRNAGVGGSNRYKIYEGYNSTEIIKDVAKQLDEFELKLKIQDESYKILLAE
                     MKKRGNIYKSLPVLQPIHVNELTRIGSFYGYRPHPILGVIQMHHGIDMVAPTGTPVYA
                     SGDGVVELVEKIRTGYGNNIVINHGVNGLKSRYAHLHTINVKKGQKVKRGELIGTVGS
                     TGLSTAPHLHYEILVNNSSVNPLRYMLVPNPSEYEQLINETQYPGKSFD"
     gene            31221..32720
                     /locus_tag="PKN53_01155"
     CDS             31221..32720
                     /locus_tag="PKN53_01155"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOD87594.1"
                     /translation="MKLNIRVNLLISLIVIALIFFCSFSFLKLPPKPDDDGIKLIIPT
                     FLGNEERNYYGNIAPESLDIIWKTTLGCGKTVFPNKDQEVRDMCGAGWTGQALLIEDQ
                     GELYLIQGAYDYNLRKIKAKTGEVVWKYPYDDVIKSTGCVWVNPNRTKDDPDKIVIFQ
                     GSRRGFKVPLHEKYIFSYRAISFRTGKELWRYNSVYGGSFSRDVDGTALIVDDTIFLG
                     LETGDFVKLNPNPDSAQIIDSYKQPVEFFKQALYTKEDIQAHRRNVVIESAPSLLDNF
                     VFITSGAGYLYKYDRNSGQIDIQFKTGSDMDGSPIITREKKVLISIEKEYIPGKGGAM
                     LIDPTKHKDSCVVWFYPTGDKHFVSWYGGIIGSCAINDRYISENENSLAAFVGIDGYL
                     YVVENELTNPYDLVKGPCDKTLYPQPQEIFKYETGPSISTPIFTKDRLIVLTYEGLYM
                     FGYDKNCNFSLLEKNTNIRGEATPIIHYGRLFVASRDGNLYCLGKVIKN"
     gene            complement(32817..34154)
                     /locus_tag="PKN53_01160"
     CDS             complement(32817..34154)
                     /locus_tag="PKN53_01160"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOD87595.1"
                     /translation="MKNSKTVQKIGLLTAISYVIANMVGTGVFTSLGFQLAGGVTDVA
                     AVMILWLMGGIIAFTGAQVYGELGSVMPRSGGEYHFLSELYHPALGFMSGFFSMFVGF
                     AAPIALASMALGTYFSNVYAGINPQYIAAGVIVLVTIVHSTSLKSGGAFQNIFTIIKI
                     LLIIAFIVAGFVFTPEPQNVSIIPSSVTWSQVLSSGFAVSLIFVYYSYSGWNASAYFV
                     SELKNPAKNLPRSLIIGTLIVTVLYLLVNFIFLYVSPVSSLTGQLDIGAIVAGDIFGA
                     KGGMIMSGLISLLLISTISSMVFAGPRVIQVIGEDYPALSFIKRKRKNGVPLVAILIQ
                     SSISLILLFTSTFEQLLSYLGFVMNIFAVLTVIGVFIHRRKYPNMSRPFKTKGYPIVP
                     IIFILFVIWNMIFMFKEKTLETLIGLATLLIGLAFYYIAKAFAKKGGVRNINS"
     gene            34229..35146
                     /locus_tag="PKN53_01165"
     CDS             34229..35146
                     /locus_tag="PKN53_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOD87596.1"
                     /translation="MKKIIPLIILTLAMCSACNNLKEKKTVENAKTTESQEITKNQEV
                     VQIPHKNDTILSYKYDCCFIVSTKIIYPPKDANIKGAILLLHGWNLPADEWCEKTSFC
                     EKALNQNYVLIIPDYSKSNYTLEIYPQTIANYKKYPTITWIMETQIAMIQESMNLLLP
                     GQNTFVAGISTGGRGATLLAYYMPNIFKAVASLSGDFDITTMTDEYIYYSFLGHYEDF
                     PERWKKECFAYDCKNFKTPIYIAHGEADRVSPVEQSKAMYDSIKLHQPNLKIKANFPT
                     AAGHNYDFWEYETDNVLKFFEEVYQELPK"
     gene            35253..35759
                     /locus_tag="PKN53_01170"
     CDS             35253..35759
                     /locus_tag="PKN53_01170"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOD87597.1"
                     /translation="MKTKLLLFALILCSSFVFGQNKDFESPAKTSKQSIILFKSLVYD
                     FGKIKHESEAKAVFVFKKVSKKPVKITNVQTSCGCAAADWSREEITNKKKQKISIVYD
                     SRIIGKFTKYAYVYVEGQDEPIQLQIKGEVLPSDTQEQAIENKTNVSKKENKTKSMNQ
                     KKKKINNN"
     gene            35775..36971
                     /locus_tag="PKN53_01175"
     CDS             35775..36971
                     /locus_tag="PKN53_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOD87598.1"
                     /translation="MPKISKKGMAMPSSPIRKLVPYAEAAKARGVKVYHLNIGQPDIK
                     TPEVALNAVKGMKDTVIAYSHSAGNISYRKKLVEHYKKIGLELTPEDIIVTNGGSEAI
                     TISFMSTLNEGDEVIIPEPFYANYNGFAIQTGVVIKPITSSIKNDFGLPALEEFEKLI
                     TPKTKGIVICNPNNPTGYLYSKEELYQLAEIVKKHDLFLYADEVYREFCYDGNTHLSV
                     LNIPGLEQHAIMLDSVSKRYSMCGVRVGWIVSKNKEVMATAMKFAQARLCPPSLGQIA
                     AEAAIDTPDSYFEEVYNEYIARRNFMVAELNKIPGVYCPNPKGAFYTVVKLPIDDADK
                     FAQWLLEDFEYNKQTVMLAPATGFYNTPGLGKNEVRIAYVLKIEDIKNAIETLRKALE
                     VYPGRV"
     gene            complement(37061..38143)
                     /locus_tag="PKN53_01180"
     CDS             complement(37061..38143)
                     /locus_tag="PKN53_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HOD87599.1"
                     /translation="MKIRRIIYRILSLCLFVFGIFITSCMKDDFRPITEFDTNSRGVF
                     IVCEGNFMYGNASLSYYSPLEKKVENTVFIRANGMPLGDVAQSLIIYDKTAWIIVNNS
                     GKVYAIDIDNFKIKGKITDLVSPRYMRFINPQKAYITDLYAKNIYVVNPQDYSLTKTI
                     NIDDGSGKYYNHSSEQIVVFENKLFINTWSYDNKILVIDTNSDEVIDSIEVFKQPRKI
                     VADKEKNLWVLSDGGSDYSDYKGDAGITKINMQTHKIEQSFVLPSKDNITDMQINKTG
                     DTIFYVNGNIFRFPINATSLPQKEWINVGHKTIYGIGQDPYNYDFYISDAVDYMQSGV
                     IYRYNSNLHCVDSFAVGIIPNSFVFK"
     gene            complement(38140..40107)
                     /locus_tag="PKN53_01185"
     CDS             complement(38140..40107)
                     /locus_tag="PKN53_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOD87600.1"
                     /translation="MIRKFLNIIIILLVGNCLFSQEIHIPDTINLIEEVFIYAPKNLE
                     ETGQKINRLDSLIIENKFLDDISSLLSENTSVFIKSYGKSSLSTASFKGTNASHTKLT
                     WNNVELNSPLMGIVDMSLIPVAISENIKIYYGAASLSKNSNALGGLIELNSSTTWSKG
                     VKAKIVSQVASYSSYDNLGEIRLGNAKVQSVTKLYHSISKNNFPFFNNDITNQQIEYR
                     QNADYLKYGFVQEVYFRFNHKNIISAKFWKQLSDRGVPGLTSNEAGPNNNKNRQNSGM
                     NVYATNYTHYGERLKFELNHGANFQNLNYKSTNYINGVGYLNVVNSFSKSNSIYNSAS
                     ANYKFNEKAELNAQINYNFHFVNSEDSIVNQYLTAKRQEGGLNVSAYYEIFKNFKLAL
                     LLRQDFCDKNLNPFVPVLSAEYFFKNNLSLRASVGRNYNLPSLNDLYLKPSGNPDLKA
                     EIGNNSNLGVSYFLNKKNFSLKTDFELTYNKINNWILWRPTAMGFWRPDNIDAVIASG
                     CETNLIFEYKLRKVKFKNIVNYAFTKSVRADENANPNDQSLGKQLPYIPRHSANLFSQ
                     VSWSNFFFNYQWTYFSTRYTSSVAEPGVLVSIYPYFMNDIGIGKNFIFKKVDLVLNFK
                     IYNLFNEKYRSVLWQPMPGRNYSLQLGIKLK"
     gene            complement(40100..41101)
                     /locus_tag="PKN53_01190"
     CDS             complement(40100..41101)
                     /locus_tag="PKN53_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD87601.1"
                     /translation="MKLSLLFSVFCFSSILLFAQEFPQGTNAIHKDSSIIVSWAVSAV
                     VERGYINIADTAFTYSQGGVTSNKAWHGKVQDALGKADGNAISLGDGGHIILEFENPI
                     FDGNGPDFVVFENAIFTPQGQTKMAFLELGFVEVSSNGVDYARFPAVTEYQFETQIGS
                     FDAMDRDFLHNFAGNFPTFYGTPFDLAEINDPNVDVQNITHVKIIDVVGNINPEYASY
                     DSQGNIVNDPWPTPFYSCGFDLDAVGVINSAVNVNEIAKNNFQIFPNPTKDFIKINSE
                     NNIQEVYIFDNQGRVLIYKNNLESNSILDLSSLNSGVYFIRIFDGNNFLNQKIIKLD"
     gene            complement(41116..42342)
                     /locus_tag="PKN53_01195"
     CDS             complement(41116..42342)
                     /locus_tag="PKN53_01195"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HOD87602.1"
                     /translation="MRKKLPLVLALMLSVSFVFAQTFVNLVDFESLTLAPESHYAGED
                     NAGGFEVDYLKFYNSYSPDYGGMWYGFAYTNETDVTTPGSTNQYSSASGSGVNASENY
                     VVGFMGFDWVSTFAPIPSVIKLSKENPEAIIPGMYVNLNTYASLYMADNDFYKTEKHW
                     YKLKIVALSTTSYFTNEAEFFLADYRSDNEEMQFKIDDWTYVDLTWAGVADSLLFYVE
                     SSDTNAYGMLTPSYYCIDDFAATCPETVPEFITDIKTNYNASVGEEIQLSAFVKGGVQ
                     PYSFEWSNAEILDDATSQMPVATVTGSTTLTVTVSDAKENTITETVNVFVETNIAENK
                     AFEPKVYINQNNILNIESENEIKSLEIYDITGKQLISNSSLNNNMNFDLSYFPQGIYI
                     VRLSDGISMYSTKIFK"
     regulatory      complement(42455..42647)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(42689..43237)
                     /locus_tag="PKN53_01200"
     CDS             complement(42689..43237)
                     /locus_tag="PKN53_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87603.1"
                     /translation="MKILVVLFLCVGFVKSAKSQYLENDPTYVQRIFYGGDIGFSFGY
                     ATYIAVNPIVGYRITNRLSSGIGGNYTFAKSNYYGYEGSMYGGNIFASFTIIKNLGNA
                     ISFLEGNGILIYAEYNVVNIKNYYSIPGREINYVASPMLGFAFQTPIGGRSYLLMMIL
                     YNFNETSISPYPNPVFKFSYQF"
     gene            complement(43268..45775)
                     /locus_tag="PKN53_01205"
     CDS             complement(43268..45775)
                     /locus_tag="PKN53_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HOD87604.1"
                     /translation="MENFENKELKLAQEFVLYTHKNIFLTGKAGTGKTTFLKNLKLYC
                     PKRMVVVAPTGVAAINAGGVTIHSFFQLPFSPFISAYTTISENSKNEQTDNSAYTYKL
                     SRNKIKLIQSLDLLVIDEISMVRADLLDAVDDVLRKYRHTDKPFGGVQLLMIGDLHQL
                     APVVKEDEWNILRDFYDSAFFFSSKALQKTELVTIELKHIYRQSDETFISLLGEIREN
                     KVSQKSIELLNSRYEPDFNLNENEGYITLTTHNANAQEINDEKLKKIKLRSKKFKAKI
                     KDDFPEYMYPTEYELELKVGAQVMFVKNDSSFEKLYYNGKIGIITKFVEDLIVVKCDN
                     QDEIYVKTEMWENVRYTLNNETKEIDEEVIGSFEQYPLKLAWAITVHKSQGLTFEKAI
                     IDVNAAFAFGQVYVALSRCKSLEGLVLISKIPSSAIKTDSTISRFNENVAENAPDQEK
                     LQDSKLIYQQNLILEQFDFLNLKKRYFSVKKIYTENILRFGEGFVKIFEEITAQAQSE
                     IFDVNDKFIKQLNYLFNTNKEELPENSEQILDRIAKASKYYIEKFNFIFISKFKSLYF
                     DCDNKEISNEMDNAINRFELELKLKLGAMQASVEKFETLKYLRALSDTEVDFKSEFNR
                     PTVYSSHTENKNLFAELNAWRKSLAEDSGIDVYKILPYKTLRELVEKLPQNLTELGKI
                     KGIGKVKLKQFGDDILDIIYEYCERNNIKRAEPVEEIISKTKAKKTQAEKLIKEPKKD
                     TKTISFELFKQGKSIEEIAEERSLVFSTIFGHLSHFVLKGELEITELVEQVRLDKIVN
                     FIEEQENLNLRDLVEKSNYEFNYNELKLIIACKEKFE"
     assembly_gap    45926..46025
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(46331..46657)
                     /locus_tag="PKN53_01210"
     CDS             complement(46331..46657)
                     /locus_tag="PKN53_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD87605.1"
                     /translation="MKMQGKNKIIVGGFYNDNFVITRLNANSKSGEDPASLEKNQLNT
                     LSIYPNPAKDNLYFNEETQAEIIDIQGRVLYKTTEAVKSVNISNLKSGIYFIKTDNKI
                     QKFVKE"
     gene            complement(46867..47751)
                     /locus_tag="PKN53_01215"
     CDS             complement(46867..47751)
                     /locus_tag="PKN53_01215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87606.1"
                     /translation="MLATSASSQSFSLDNTFGNQGVITSVDASDVYFIDFDDNGNIIA
                     IASKIGSQPSDYHYLSIIKTDANGVVMETFGNEGVMILTDYANARIYSSQITNENKIL
                     VFGYFSETYFGDSQAMFMQFAENGNLDESFGNNGKIIINTALNDDVYANCASFGSSDF
                     LLVGKRNHLDFSEVKCYISKYNYDGNLDESFGQEGNAYLTDNATYRIVPLSIYVLDDQ
                     SVLVAGFNEFKGDGNYELALCKLDQNGNLVTDFANNGVWLMDIVQDFNLDYEYFNKII
                     ENNGDIIVSGKGKEGKLF"
     gene            48051..48503
                     /locus_tag="PKN53_01220"
     CDS             48051..48503
                     /locus_tag="PKN53_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HOD87607.1"
                     /translation="MFLEGILSISGQAGLFKLISKGNNNVIVESLNTGKRMPTFTSSR
                     ISTLSDVAIYTDDNEDISLREVLIKMFEKFNGETIKIGKDSNKELAQMLESVLPNYDK
                     SRVYISDIKKLTNWYNSLIEHKILTKENIKIESEEAKKSAEKENNATE"
     gene            complement(48594..49169)
                     /locus_tag="PKN53_01225"
     CDS             complement(48594..49169)
                     /locus_tag="PKN53_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOD87608.1"
                     /translation="MSIIGTKTEQNLLKAFAGESQARSRYTMFAKTAKKEGFEQIAAI
                     FLETAEQEFEHAKTFFKYLESGENLEITAMYPAGKVGTTAENLKAAAMGENEEWTELY
                     PAFADIAEKEGFKEIATSFRMIAKGEAEHEKRYLKLLDNVKNNKVFEKEEEVEWMCRN
                     CGYVHKGKKALKTCPACKHPEAYFEIHSVNY"
     gene            49300..49803
                     /locus_tag="PKN53_01230"
     CDS             49300..49803
                     /locus_tag="PKN53_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOD87609.1"
                     /translation="MLNSKIEKILNEQIEKEGFSANLYLAMASWTETLGMSGTASWLY
                     AQAEEEKMHMMKFVSYINERGGHAIIPAFEKPASSWKDIFVMFDEVLKHEQYISKSIN
                     DILELAIKEKDFATQNWIQWFVSEQVEEESSVQGVIDKLKLLGKASLYMFDRDIMSFR
                     NDVEDAE"
     gene            49875..51305
                     /gene="hydG"
                     /locus_tag="PKN53_01235"
     CDS             49875..51305
                     /gene="hydG"
                     /locus_tag="PKN53_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOD87610.1"
                     /translation="MKFVPERYKIKDERMKSFIDTEEIEGYLEETKNPSKEEVLAVIE
                     KSLNKNRLNLRETAILINAESPELINLIKEGARTLKEKIYGNRIVLFAPLYIGNKCKN
                     NCLYCGFRISNKDANRKTLSDQEIVKEIEALEDNGQKRLILVYGEHDSYNPEYIAHTV
                     RLAYSVKKGRGEIRRVNINAAPLDIEGFRTVQRSGIGTYQVFQETYHQETYKKYHVAG
                     PKKDYDYRLTSLDRAQEAGLDDVGIGALFGLYDWRFEVLALVRHVNHLEACYNVGPHT
                     ISFPRIKDASGLNIDDKYLVSDEEFVRLIAILRLAVPYTGLILTAREPAVIRDEVMRF
                     GVSQIDGGTKLELGSYANSQNEKQDLNKEQFEINDTRSLGEIIGELLDKDYIPSFCTA
                     CYRLGRTGEHFMEFSVPGFIKRFCTPNALLTLTEYLIDYASDEVKEKGWKQIDKEIAK
                     IEDPKLAESIKEKIEKIKQGERDLYY"
     gene            51310..51570
                     /locus_tag="PKN53_01240"
     CDS             51310..51570
                     /locus_tag="PKN53_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87611.1"
                     /translation="MKKELNIVVIKIADRIKEAGLVQKTLSDYNEIIKTRFGFHELST
                     EVCSRQALIILELAGELEQEKKLVSELNQIGGIKTEVMNFEL"
     gene            51573..51809
                     /locus_tag="PKN53_01245"
     CDS             51573..51809
                     /locus_tag="PKN53_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87612.1"
                     /translation="MEYNIVSILVKQPQLDASKLQNLFRDYGCIIRSRLGLNQTAVKG
                     GIIILELSGDSNQIDLFLEDLKTFKGIEYKFVKI"
     gene            51873..52307
                     /locus_tag="PKN53_01250"
     CDS             51873..52307
                     /locus_tag="PKN53_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOD87613.1"
                     /translation="MTKILALSEANAIAFHGMALIAKSKVAISATEIAELTNSSKHHT
                     SKVMQRLVKSGLIGSTRGPSGGFFLKKKPTEIKAIDILEALEGKVDLKDCPIDNIHCP
                     FGSCLLGNVCNEIGTKLINHLQTHTLQDMINNIANEDILVKK"
BASE COUNT        18612 a         8489 c         7538 g        17649 t
ORIGIN      
        1 attattaaag cagatgaaaa tatctattac ctaaaaattt agggtttgag tttaactgct
       61 cgttttacgt ttatctaatt cctgcatttc tgagtgcttt gtgatatttt cgagcattgg
      121 cttcgtgttg tgcatttgta acggcaaaca catgataacc cgaaaaatcg tcttttgcac
      181 agaaaaactt ataattatgt ttttcgtaat tcagaactgc atcaatagaa gaaattgatg
      241 ggatatttat tggtcctggt ggtagtccgg agtatttgta ggtattataa ggcgattcaa
      301 tttccaaatg tttatttaaa actcttttca aagtaaaatc tcccacagca taaacaacag
      361 ttggatcggc ttgtaaaagc atgccaagat ttaacctatt aatatataca cctgcaacac
      421 gagctttttc gtcattttgt tgagtttcaa cttctacaat agatgccaaa gtagaaactt
      481 gagattgagt catgccgata ttttctgctt tttgcaatct ttcttctgtc caaaaagctt
      541 taaattcttt tgccattctt tctacaaact gctcagccga agtattccaa taaaactcat
      601 aagaattagg aataaacata gccataaaag ttcgctcatc gaaaccatat ttttgagctg
      661 tttctggaga ttttaatagt tgagcaattt ccgtagagtc ggcttcaata tttgcagcta
      721 cttttcccgc caaaatatca aggctacgaa cagatataaa acttactttc acaggagttt
      781 gctttccact tcttagcaaa tttaccaaat cattattcga cattccatta cgaatttcgt
      841 aacgcccagc aaaaactttt gaaggataat ttttcttttc tgccacaagt ttaaaagatt
      901 ccatatcttc taaatattct gtatcttcaa gtatttgcaa aacatcttca taatttgaat
      961 ttgtagggat atagagataa gcagtttttt cttctaattt aacatttgga gcaaaagcat
     1021 aatcatagaa ttttttagca aaaactccag caattgctgc aataatcaaa aaaactacta
     1081 atattattat aattattctc tttctgttct ttttcttcgc tttgcccata gttttaaaat
     1141 tatttgaaaa gcacaaaaat attaatttta ttcataaatt aaatatttat ctcttattct
     1201 tttaaaattt tctaaatcat cttgccaaga ttctctaatt tctttttcgc ttactccgct
     1261 ttcaacttgt tttctaaact ctccacttcc tgaaagaagc tctaaagagt ttgatttttt
     1321 gaaaaattca cttttttcag ggaatagctc atatgtttta agtgttaaat ccaaattcaa
     1381 ctgattttct ttaatttcga aaatagaatt tgaatcagac aaatcaaaac caaagcattt
     1441 ttttccattt agaacaggat ttgaagctcc ttcgtttggc attggagtaa attcaaaatt
     1501 tgcttgttca acattctcta acaaaggatg tccaataatt tgaaactgtt tatttgttcc
     1561 tcgaccaacg cttaaatttg tggcttcaaa aaaacacaaa cttggatata gctcaatagc
     1621 tctatcgttt ggcaggtttg gagatggttt tactggcaaa gaatattttg aattgtgagt
     1681 ataattttta caaggaataa ttgttaaatc acattttacg ccattgccta accaattttc
     1741 tccatttatc atttgtccgt actctcctat tgtcattcca taaaccactg gtactgggtg
     1801 cattccaata aaagagcgat aatttgcagt atctaaaatc caaccatcaa cataatgagc
     1861 atttggattt ggtctatcca atacaattac tggaatattt gcttcggcac aagcttccat
     1921 aacataatgc aaagttgaaa tatatgtata aaatcttact ccaacatctt gcaagtcaaa
     1981 aacaagaata tcaaaatctt gcatttgttc tggtaaaggt tttttatttt ttccatacag
     2041 cgaaattatt ggcaaacctg ttttttcgtc tatagtatta tcaactttag caccagcatc
     2101 ggcagttcct ctaaaaccat gttctggaca aaaaactttt actacatcaa cccccgaact
     2161 caataaagta tcagccaaat gaacattatt gataattcca gtttgatttg aaacaatagc
     2221 aacttttttg tctgacaaca aaggcaaata ttcttgagtt tgataaatac caggtaatat
     2281 tttaggatta tcttcttgtt tttctgtaac aatagttttt accaattctt catttctaag
     2341 tcgatttgta caacaaacaa aaattataaa aatcagacta atcgtaaaac atgttttttt
     2401 catatcatta attttacaca aaaataattc aaaaatttga atgataaaaa tacttaatat
     2461 tgtgttaaat tttaaaaact tattgttttt ataagtattt ttgggactaa gaacaaaaca
     2521 attatatttt tatgaagtct gatttttata ttttaatcat tttttcaaca atattaatta
     2581 cattttcttc gtgtaataat caaaaaacta ataacgtgga ttttgcatta acctaccccg
     2641 aaactaaagt taccgatgtt aaagacaact attttgggac agaagttgcc gatccttatc
     2701 gctggttaga agatgataat tctgaagaaa ctgcagaatg ggtaaaagct caaaacgaag
     2761 tgagttttaa atatttagaa tctatcccat atagagaaaa aattagagat agattggtta
     2821 aaatttggga ctatcctaaa gtttctgcac ctacagaaaa agatggactt tatttctatt
     2881 ttagaaacga tggacttcaa aaccaaagtg ttttatacta taaaaaaggc ttagaatcag
     2941 aggaaataga attattagac ccaaacaatt ttgataaaga aggaacagta tcgctttcta
     3001 atgtttgtat ttctgaagat tgtaaatatt tagtttatgc tatttcgcgt gcaggttctg
     3061 actggagaga aatttatgtt agagatattg aaactttaaa agaccttgac gatcatcttg
     3121 aatgggtaaa attttcatct atagcatggt taaatgatgg attttactac actaaatatc
     3181 cagaacctga aaaaggacaa gagctttctg gcattaacac aaattcaaaa atttattatc
     3241 acaaagttgg cacagaccaa agtgaagata ttttaactta tgaagaccct aaaaatccag
     3301 aaattggatt ttctgcttat gttactgatg acgaaaagta tttaataatt catgctacaa
     3361 aatctacatc tggaaattca ttatattaca aagacttaga aaaaccaaac tctactttcg
     3421 taaaacttgt tgacgatttt gataatgatt atactgtaat tcattcaaaa gacaataaac
     3481 tttatgttta tacaaactat caagctccga tgtataaaat gatagaaatt gatttaacaa
     3541 agcctgatag aaaattttgg aaagacttta ttgcagaaga taaaagtgtt ttagaaactg
     3601 taaattatat tggtgaaaga tttattgcta actatatggt tgatgcgcat tcagtagtta
     3661 aaatttttga cgaacaaggt aaatttttac acgatttaga taataaagaa attggaacta
     3721 tatatggttt ttctggcgat aaagatagtg atgttacttt ttacacattc acttcattta
     3781 caacaccagc agttatatac tcatatgata taaaagcaaa taaatcaagc gtttatgaaa
     3841 aagctaacat aaactttaac acagacgatt acgaaacaaa gcaaattttt tatacatcaa
     3901 aagatggtac aaaagtgcct atgtttattg ttcataaaaa aggaattgct ttagatggta
     3961 aaaatccaac tttattatat ggttatggag gtttcaatat ttctttaact ccttcattta
     4021 gtatttctcg ctgtgtgtgg ttagaacaag gtggtattgt tgcaattcca aatattagag
     4081 gtggaggcga atatggcgaa gaatggcata aggctggtac tttaatgaga aaacaaaatg
     4141 tttttgatga ctttattgct gcagcagaat atttaatttc aaatcaatac acatcaccag
     4201 aacgacttgc tattcaaggc ggttctaatg gtggcttgtt ggttggagct gttgcaaacc
     4261 aacgtccaga tttattcgct gtagcactcc ctgctgttgg tgtaatggat atgcttagat
     4321 accacaaatt tactattggc agatattggg caacagacta cggaactagc gaagacagca
     4381 aagaaatgtt tgaatattta tataaatatt cgcctctaca taacataaaa cctgatgtag
     4441 aataccctgc tgttttagtt actactggcg atcacgatga cagagttgtc ccagcacatt
     4501 cgtttaaata tattgctact ctgcaagcca cttacaaagg cgaaagacct gtactaatca
     4561 gaatcgaaac tcaagcagga catggtgcag gaaaaccaac taaaaaaatt attgacgaaa
     4621 tcgctgacac ttatgctttc actttttata atatgggatt tacgccagta tttcaataag
     4681 tttacttttt tttcataact atcataaaat aaaaagtcca aattttgaaa tttggacttt
     4741 ttgcattaat aaattttgta tattagcaga aaatttaatt tgtaatttat aaaaaataat
     4801 atctatgaaa aaaatttcaa ttatcattct tgctacacta ggctttagca tgatattttc
     4861 ggcttgtaaa aaatacgaag aaggacctgc actaacttta cgaacaccta aacaaagagt
     4921 tgttggcact tggaatctaa cagaaactag atacaataat gtaaaaatag attttaacaa
     4981 cttgcaagac tttttcaaca gttttgattt aggcggtcta gatttgagta atgcaccaga
     5041 ttctaccaca tttgatttaa gtcaattatc tctgaaatca attaaactaa atcttgacaa
     5101 agaaggaaat ggagctataa gtattgaact tgcttatgga atgttacagt ttccatatag
     5161 tcaagaaatt acttggagct ttgatgacaa aaaagatgca ttaattttta aaataaaaga
     5221 tgttgctgaa gaacaaagtt tcaaaatttt gaaacttaca aaagatgaat tatggttaga
     5281 aaaaaccgac gtcaacaatg gagttgctag tattgtcaac tttgaatttg aaaaaattaa
     5341 agactaaata aacaaaaaac tttagagaaa attataaatt agactaataa ttgtgtgtct
     5401 taaaaacaga agatgattaa taaactttat agtctatttc ctccattctt aaaacgtttc
     5461 gctatttttt ttcatcgcac attgcgatta ttttacttta atttaaccaa aaaaaaatct
     5521 tataaaaatt gttcatcaat ttcacaaaaa gtttttgaag aatttaataa atctcgccct
     5581 tatggaccat taaaaaaaat atgctatgct cctttttcaa gcatgttttt ttctcgacat
     5641 ggcttagtag ctccgtgtta tgctacatat aacgaaaatt catctcatat ctctaataat
     5701 tctatcaaag acatttggtt ttcgggaagt ataaacgaaa ttagaaaaca acattccaga
     5761 tgtgatttat caacatcatg ttctttttgt gaaaatatac ttaattccaa ttcttataaa
     5821 tcagcattaa ttaataaata tgaacattat gctttttcga aatcgaaata tccaaaaata
     5881 atggagtttg aactttcaaa caaatgtaat ttagcttgta taatgtgtga tactaactta
     5941 tcatcaagta ttgaaaaagg gaaaaatcaa aattttactg gcaatttatt ttacaaagaa
     6001 agtttttttg aagaattgcg agaatttatt ccacatttac aattagctga atttactggt
     6061 ggcgatcctt ttatgattga agaatactat aaaatttggg atattataat ggaattaaat
     6121 ccgaaatgtc gtattttaat aacaacgaac gcaaacacaa tgaatccacg catagaaaga
     6181 ttaatgaata tgcacaaaaa cttgtatttt aatatttcaa tagattctct tcaaaaagaa
     6241 aattatgaaa aaattcgaat aaacgggaat tttgaatatg caatgaaaaa cactcaaaaa
     6301 tttattgatt attgcaaaaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 aattttgaaa atttgataga gcttttaaaa tcttggtcta aagaaaatga attagacata
     6481 aaacatgaca atatcgaaga aaaaacctta tccgaaactg aaataatcaa tttgctcaaa
     6541 ctgaaagtaa atgagacaaa gcctgaattt tttaaaaagt ttgataattt ttcacaagaa
     6601 gttttacaat tacccaataa agatataatt ttaaaaaaaa tttataattt ttctgatgaa
     6661 atattttttg aatatttgga aaataaaaat attgatgagt tgatagaaat ttgtaaaact
     6721 ataaaatgaa aaataaaata cataatatag aattttttgc aagaacaaaa attaaaaagt
     6781 tttacaactt gattaagtat tttccaattt caaagcatat taattcaaat attgagaata
     6841 aaaaaaacct tgtaaaatat aactacagcc gaagtatagg tgtcgagcca tgtgtttgtc
     6901 atgcaccttt gcgaagcata tatttcgata tgaaaggaaa tgcaacagct tgttgtttta
     6961 atagaacaca tattttagga aaatatcctg agcaaagtat taaagaaatt gtatttagtg
     7021 aaaaacgcga ttttttacaa aaagaattat gtaagcaaaa cttcatgtat ggttgtaaac
     7081 actgtctcaa tctcattgaa gctcaaaact ttgaaggtgt tgaagccaga ctttatgata
     7141 atttaaaagc tcaaggtaaa atgcctagcg aaatgatttt cgaacttgat aatacatgta
     7201 atcttgcttg cgaaatgtgc cacgaaggtt tttcttcaac aattgctaaa ctaaaaaaca
     7261 taaaagttcc accacctccc tacgattcaa agtttctaga acaattaaaa gaatttattc
     7321 cacacttaga agttgctaaa tttcttggtg gagaaccttt tttaataaat atttattacc
     7381 aaatttggga tttaatttta gaactcaatc ctaagtgtag aattcactta caaacaaacg
     7441 gaacaatatt taatgaaaaa attaaatctt atttagaaaa aggcaacttt tatattggcg
     7501 tttcaataga ttcattagat ccagaaaact ataaaaaaat aagacaaaat tccaatcttg
     7561 aaaaagtaat aagtaatctt tttaaattcg ctgaaatttc aaaggaaaag cacactttta
     7621 taaacatttc tgtttgtcca atgctacaaa attggaaaga agttcctcaa attgttgact
     7681 tttgtaatca aaattcattt ttcgtatatt tcaacactgt ttatacgcca ggttttgcta
     7741 ttagtgatgc agatgaagat ttattattag aaatcataaa ctattataaa aattttactt
     7801 ttaaaactaa aggaataata gctaaacgaa acttaaaatt tttcaataac ttattaactc
     7861 aaattaaagc tatttataaa cccaaagcag aggcttcaga atacgaaaga aaaagacaca
     7921 aatggacaag caacatgcta aagaatttta tgctaaagaa agttaacaat gataaagaac
     7981 tatcaagcaa aatcgaagaa ttatttacaa atactaataa tgagttttat ttttctgata
     8041 aagatttaga aaacttagaa aaactaaaaa ttgaagattt aattactgct ttaaaaaatg
     8101 aaactctcat agaattacaa gaaagaatca agcggtttat taattttggt agatttagca
     8161 aataatttat cttttaaacc caattgtttt ttattataag tatcatttgt caatttcgta
     8221 atattttata cttttgacaa aaaattttaa ttatgaaaca agtttggatt ttagtatttc
     8281 tcttttcgtt tgtaatatta tcatgtaata ataagaaaaa agaagctcaa gttgctgata
     8341 atgacacaga aactattgaa gcacctattc atgtttatga cacattttct tacaaaggtt
     8401 ttaatgaaat agcaaattac ataggtggca ctcaacctga agaagaagcc atttattttt
     8461 ctaagtattt ttcagaccct gattggaaaa attatgcaaa tgcaaatagt gaaagttgga
     8521 atcttcatag tcaacaagta ttagacattg tttctgattg gtcagaaaaa aacttaactt
     8581 atggtgacac agtattttat ccttttggtg gtccagattt taattatctc gatgcaatgt
     8641 tcccaaaagc taaacatttt gtaatgatgg gattagaaaa tgttggcgat attcctgact
     8701 tagaagatat agaaaaatac ggtacaaaat cttatttatc aaatatacaa caagcaattc
     8761 aacacaactt aaatttaagt ttttttcaaa ctaattcaat gaaagttgta ttaaactctg
     8821 atattatcaa tggaacgatt ccaattataa tgtattttct aaatcgtcat gataaagtta
     8881 ttgtaaatat aaatccagca agtataaatg aacaaggcga tataagtgtt gaaaatccta
     8941 atgaagaatt tgcatataac tacaaaaaga aatttaataa tggtgttgaa tttgtttatc
     9001 gtgaaaaaga cacaaaagaa ttaaaaaaac ttacttatct aagtcttgat atttgcgaca
     9061 taatgtttac caaaccacat gaactacaat tcataacaaa ctacacaaaa aacaatactg
     9121 tttatctcaa ggctgcgtca tatttatgct ttagagacac attttcaaaa attagaaata
     9181 taattttaga taatgcttcg caagttgtta ccgacccatc tggaattcca tacaaatatt
     9241 taaagtctaa atttgatgta aaactttatg gcgaatataa tggtccaata aaactttttg
     9301 cagaaagaaa ccaaccagat ctaaaacaag ctattgattc cattaagcct ggtcgtcttc
     9361 cttttaaata tggttaccac cctacccatt ggtgcattat ggttgccacc aaaaggtaga
     9421 atggtatatt aaattttata tttataagtg tactgcttcc ccatgtgcag ctgctgctgc
     9481 ttccattatt gcttctgata aacttggatg tggatgaata gaatttatta aatcttttgc
     9541 tgttcctttt aattttctta aaactaccat ttcagaaatc atttcagcaa tattatcacc
     9601 tacgaaatga gctcctaaaa gttcatctgt ttcttcgtca aataaaagtt ttacaaatcc
     9661 atctctaaat ccacttgcaa cagctttacc agaggctata aattgaaatt ttccaacttt
     9721 ataagctttt cccatttctt ttaatttttg ctcagtaaat ccaacagaag caacttcagg
     9781 agttgtataa acacatgaag gaatgttaga ataatcaaca acaggtggat ttaaacctat
     9841 aatattttca acacagcaaa ttccttcagc cgaagcaacg tgtgctaaag catgacctgg
     9901 aactatatcg cctatagcat aaactccatc tacatttgtt ctataatatt cgtcaactaa
     9961 aattttccct ttttcagttt ttattcctaa ttcttcaagt cccaagtttt ctatattagc
    10021 tgttacacca accgctgaaa gaacaacttc acattctata gtattttcac cttttttgtc
    10081 agtataattc acaatacatc taccatcaac tacatcaaca tttttcacag cagttcctgt
    10141 catcacttta attcctgctt ttttaaagct tcgggctagt tgtttagaaa cttcttcgtc
    10201 ttctaatgga actatttcgg gcataaattc aattaatgta acttctgcac ccattgaaga
    10261 atagaaaaac gctaattctg agcctattgc tccagaacca acaactacca ttgattgagg
    10321 aagtttttct aaacataagg cttcgcgata agttataata ttttttctat ctatagcgat
    10381 tcctggtatt tcgcgtgaac gagcaccagt ggctaaaata atatgctttg ccgaataagt
    10441 ttcagaatta ttttcactat ctgtaacaac aacctcacta gcagatttta attttccata
    10501 accacgaata actgtaattt tattcttttt aaataaaaac tctattcctt tactcatatt
    10561 tgcagaaaca tcacgacttc gagatattac tttagcaaaa tttggcttag ctgtaccatc
    10621 attaataata ccataggctt cgctattctt aacataattc atcacttgag cagattttag
    10681 caaagctttt gtagggatac aaccccaatt taaacaaacg ccacctaact cagatttttc
    10741 aaccactgca acagaaagcc ctaattgagc tccacgaatt gctgcaacat atccaccagg
    10801 accagaacca attactatta agtcaaaatt cataattatt aatatttttg aaaagtttaa
    10861 aaaagatttt gtcaaatata atattttttt actatatttg tttatattaa ataaattata
    10921 tatttttaaa aatatttatt ttacaaagag aataaaacag tttaataaaa aattaaattt
    10981 atggaaagaa atatcttagt tccttatgag tttagcaacg aatctaacta tgctttagac
    11041 cacgcttatg aattagctaa aacttcaaat tatcctattc acatgcttta tgtggcatca
    11101 aaccatgaag atgctgatga atggctaata gaattagaaa aagttgctaa aaaattttct
    11161 gaagaacata ataaccacaa aactgtagct acagtaaggg tcggaaatct ttttaaaacc
    11221 attaatgatt atggcgaaga aatcaatgcc tactttgctg ttatgggaac tcacggcttg
    11281 caaacaattg ataaagctat gaaactcatt aaaaaatttg atagaatgcc atctattcta
    11341 attcaaaaac ctatgcatta tggcgaatat gacaagattt gtgttcctat cgattctgac
    11401 aaaaaatcaa gagctaaatt tttatgggta aaatacttat caaacatatt tgaatctaaa
    11461 gtctatgttg tacatccaga ttttaaaaat tcgctaagaa agcaaaactt aaacagcaat
    11521 ttaaactttg ctggcacaat tttcgaaaga gaaaatatag attttgaagt gaaagtgttg
    11581 ccagaagaaa attatgccga ccatttatat gattacataa gtgaaattga agctgacatt
    11641 gtgttattta tgacagataa atacaaaaga attattacta atgtacaacg cccaagaaat
    11701 atagaacttt caaaaaaaat acctatcatg tgtgtaaata taagaactga catacaaaaa
    11761 ctaggtggtt ttacttatta attaagtttt aacttttaac agcaaatttt atacttttat
    11821 aaacatttaa tattttaaat gttgtaaaaa aattatatat ttgtgggcat gaaaaataaa
    11881 gaaattaatt ttaataaaac ttaataacaa actttaaaat gacaaaaatt aaaagagttt
    11941 acagctttgg aaataaaaaa gctgaaggag atgcaaaaat gaaggagctt cttggaggta
    12001 aaggagcaaa cttagccgaa atggctttaa tggggttacc agttcctgct ggttttacta
    12061 ttacaacaga cacttgcacc gaatattata acaacaatca cgaacttcct gaaggattga
    12121 tggacgaggt gtgggctgct atgaaaaaag tagaagcaga tatgggatta aaatacgacg
    12181 accctgataa tgccctattg gtttcttgcc gttctggtgc acgtagctca atgcctggta
    12241 tgatggatac agttttaaac atcggtttaa gtagcaatac aatccctgga ttaatcaaaa
    12301 aaactaacaa tccacgcttt gtttacgatt cgtatcgccg cttaattatg atgtatgctg
    12361 acgttgtaat ggataaagca gaagaccttg acaaaaatat tcgtcgccaa ttagacgata
    12421 tgtttgaaga atataaagtt caaagaaatt ataaatcaga tacagaattg actgctgaag
    12481 acttaatgat tatttctgat ttattcaaga aaaaaattaa agctgtttta ggtactgaat
    12541 tccctgatga cgcaaaaaaa caattagaag gtagtattaa agcagttttc aaaagctgga
    12601 atggcaaaaa agctgtttcg tatcgtagaa ttgagcatat tccagatgat tggggtacag
    12661 cggttaacgt tcaagctatg gtttttggaa atatgggcga aacttcggct acaggtgttg
    12721 catttactag aaatccagct accggtgaaa acttattcta tggagaatgg ttagttaatg
    12781 ctcaaggaga agacgtagtt gcaggtattc gtactccaaa cccacttaac aacgatacta
    12841 aaaactctca aaacgagcat ttaatgtcaa tgcaagagca attcccagca atatataaag
    12901 aacttgacga cattagaact actcttgaag atacatttaa agacatgctc gatattgagt
    12961 ttacagttca agaaggtaaa ctttatatgc tacaatgccg tgtaggtaaa agaactggaa
    13021 cagctgctct aaacatggca atggaaatgt tagaagaagg cagaattgat gaaaaaactg
    13081 caattttacg cgttgagcca agtcaattag acgaacttct tcacccaata gttgaccctg
    13141 cggcagagaa aaaccacgaa ccaatagtta aaggtttgcc tgctggacca ggtgcagcta
    13201 ctggaaaagt agtatttact gccgaagatg ctgttgaatg gacaaaaaga ggagaaaaag
    13261 ttcttttagt tcgtaaagaa acaaatcctg aagacgttga aggtatgaga gttgctgacg
    13321 gacttttaac tacacttggt ggaatgactt cacacgcagc tcttgttgca cgtggctggg
    13381 gaaaatgctg tattgttggt gctggtaact tagaagtaag agaagacgaa aaaatagcaa
    13441 tcgttaaagg aactgatatt gttattaaag aaggcgatat ccttactctt aatggtacta
    13501 caggtaacgc atataataca gttattaaac taatggactc ttcagaaaat ccattattcc
    13561 aaaaatttat gactttagtt gataaacatc gtaaaatggg cgttcgtaca aattgcgaat
    13621 ctccagaaga tgctaaatta gctatagaat ttggtgcaga aggtattgga cttttccgta
    13681 cagaacatat gttctatggt aaaggtgctg atgagccatt atttattctt cgtaaattaa
    13741 ttttagcttc gtcaaaagaa gaaagaatag ctgctgttaa cgaactattc ccattcgtaa
    13801 aacgcgacat gaaagacaca atggctgtaa tgaacggtct tcctgtaact ttccgtttat
    13861 tagacccacc tctacacgag tttgctcctc atacaaaaga taaacaagca gaaatagcaa
    13921 atgctattgg tatttctgct gaagaagtag ctcaaagagt tgatgctctt agcgaaacca
    13981 acccaatgat gggacatcgc ggagtacgtc taggaattac ttatcctgaa attacagaaa
    14041 tgcagtttag agctttattc gaagcgactg ctgaactcat gaaagaaggt aaaaaagtta
    14101 ccccagaact tatggttcct gtaactgctg atgtaaaaga attagatatt acaaaagttt
    14161 tatttgataa agtaaaaaaa gaagttgaag ataaatttgg aattaaatta agctgtgctt
    14221 atggtactat gattgaaatt cctagagctt gcttattagc taacgatatg gcaaaaacag
    14281 ctgaattctt ctcttttgga acaaacgact taactcaaat gacttttgga tttagccgtg
    14341 atgacgtaag tggttttatg cacgattatt tagaaaatca aattattcct gttgacccat
    14401 tccaaactat tgaccaagca ggtgttggac aattaattga aatggctgtt gtaaaaggac
    14461 gtgctaccag aaataatctt aaaataggta tctgcggtga acaaggtggc gacccagcat
    14521 ctgtagaatt ctgctacaaa atgggcttaa attatgtttc ttgctctcct tacagagttc
    14581 ctattgcacg cttagctgct gctcacgcta gtattaaatt ttaataaaaa ttgatatgga
    14641 attagaaggt aaattaattg taaaattaga tccagtaact ggtgaaggca gaaatggtcc
    14701 ttggaaaaaa caaagttttg taatcgaaac attagataat tatccaaaga aagtttgctt
    14761 tactatttgg ggcgacagag ttccattaaa caattttaca gaatcagaca ctataagagt
    14821 tttatttgat gccgaaagtc gtgaatacaa cggcaattgg tacactgacc ttaagtgctg
    14881 gaaaattgaa cttagctctc caggcaatgt aggacaagca cctatggaaa ctccaccttt
    14941 tagtgctgat gatgctcctt tcgagctaga tagcgaccct ggtgatgatt taccattcta
    15001 aaaacaaata aaaaaactgc gattaaattt agtcgcagtt tttttttata taaaactaaa
    15061 atatctaaat caacaatgat aaaaaatcat ctattatttt tttatgatca aaagctaaat
    15121 ctggtaactc atttaaacta aaccatgcca actctttagc atcatctcca gcttgtgcta
    15181 attcaggcga atcacaatat ccataataaa caaaagttat agttcttcca cgtggatcac
    15241 gtcctggatc gccgtaaact ttaaattctc tcaaattaat attgtccaat gatgtttctt
    15301 ctagaacctc tctttttaca gattctaaca aagtttcctc catttcaata aagccacctg
    15361 gcaatgccca aaaattctta aaaggttcat tcaacctttt tatcaataat attttatttt
    15421 cttttttcga aataattata gcatctacag tatttagtgc gcgtggatat tcgtaattaa
    15481 aactcatgct tattaaaatc aaaattttta ttaaaaacat atcttgtctc agaagctgtg
    15541 atttccaaat ctaaaatatt cattattatt aaatttgtta aaattttatt ggctacatag
    15601 ctttttattc ctgtatgcct aacaaactga gattttgaaa tactgccatt ttgattaatt
    15661 tcctttaata aaaaagaaat attttcatca aaattaattt tcactccctt tacattattt
    15721 tgcagacgtt tccaaacttc gactaatggt ttaggggcta taatgttttc atcatttact
    15781 ctgacataag ctttaaaaac tccatcgttg tcgggggcag tatgaggttt agtttcactt
    15841 tcagaaacta ttatttctaa aacctgtttt cctaaaacat taaaattatt tacagaaaac
    15901 tttatagaag gtttacaata aataagtgca gcagtttcta ccataaaata ttcttcttcg
    15961 gaatttactc ctgcaatact tccattatcc ctcactccta tcaatagact tccacctttt
    16021 gtatttgcaa aagctgataa agaacgtgca atttttttag catcatttat cgaatgctta
    16081 aagtctaaat ttagtccttc accttctttt attctttgta atattttatc tgcacttttc
    16141 aaaacaacaa aaagatgctg ccttagaagt acccaagttt ttaacttggt aacgcagtaa
    16201 aaaacttcta agacagcatt tattttttta atctctaaat aattttgaga tagttttaat
    16261 tataattatt tattcaaatt ttttaaaagc ttcttttacc aattcaatag cttcacgcaa
    16321 ttgttcttca gtaattataa gaggtggtgc aaagcgaata atatttccgt gagtaggttt
    16381 tgctaacacg ccaagttctt tcatttgcat acaaacgtct aaagcagttt ttcctggttt
    16441 attattaatt acaacagcat ttaacatacc tttacctcta accaattgaa tcatatcaga
    16501 ttttatgctt ttcatttcgc ttctgaaaat tttgccaaga tattctgctc tttctgctaa
    16561 tttttcatct ctaacaactt caagagcagc agtagcaact tttcctgcta atggaaaacc
    16621 gccaaatgta gagccatgtt cgccgggttt tattgtaagc ataatatcat catcagctaa
    16681 aactgccgaa acaggaataa ctccacctga aagtgctttt ccaagtataa taatatctgg
    16741 acgaacattt tcatgatcgc aggctaataa ttttcctgta cgagcaatac cagtttgtac
    16801 ttcatcagca ataaataaaa cattatattt ttcacaaatt gctttagttt ttgctaaata
    16861 tccatcatct ggaacaaaaa cgcctgcttc gccttgaatt ggctctaaaa gaaaacctgc
    16921 aacagttttt ccatgttctt ttaaaacatt ttctaaagca tctgcatcat tataaggaat
    16981 acgaataaat ccaggtgtca aaggaccata attagcataa gaatctggat cactcgacat
    17041 agttacgatt gtaattgtac gaccatggaa attgttttca caacaaataa ttttcacatc
    17101 atcatttgga attccttttt ttacaactcc ccaacgacga gctaatttca aagctgtttc
    17161 aacagcttcg gcacctgtat tcattggtaa aactttgtca taaccaaaat attcggtaat
    17221 aaatttttca taatcaccca aagttgaatt ataaaaagct ctcgaagtca aagttaattt
    17281 ctttgcttgt tctattaaag catttataat tttaggatga caatgtcctt gatttacagc
    17341 cgaatatgca cttagaaaat caaaatattt tttcccttca acatcccata aaaaaggacc
    17401 ttcacctcta tcaagcactg caggtagtgg ttggtaatta tgtgcaccat attttgcttc
    17461 taaatcaata aaatcttgcg aagttctttt aacatctttt gacataatat aaatatttta
    17521 agttttacaa taattttttc aaacaacaaa aatgtaacgt ttttatcgaa ataaaaataa
    17581 taaatctcag ttttttattc aatctgctat ttaacataaa tttattaaca aatagagact
    17641 taaaaacaaa tttatcacgg agctaaatta taaaactttt attatttttg caacttagaa
    17701 attgatttta gagtaattat gtttgaaaat ttatcggaac gattagaaag gtcttttaaa
    17761 ttattaaaag gcgaaggtaa aattaccgaa ataaatattg ctgagacttt aaaagatgtt
    17821 cgtagagctt tgcttgatgc tgacgtaaac tataaaactg caaaaacttt ttgcgatgat
    17881 gttcgcgaac aagcaattgg tcaaaatgtt ataaaatcat tgaagcccaa tcaattaatg
    17941 attaaaatag ttcatgatga acttgcaaag ttgatgggtg ggaaagctgt tgatataaat
    18001 attaaaggaa atccggctgt tatattgatg tcagggcttc aaggttcggg taaaactact
    18061 tttagtggta aattagccaa tatgctaaaa actaaaaaag gcaaaaatcc acttttagtt
    18121 gcttgtgacg tgtatcgtcc agctgctatt gaacaaataa aagtacttgg cgaacaaatt
    18181 gacgtaaaag tttatgctga agaagacaat aaaaaccctg taaaaatcgc tcaaaattca
    18241 attaaacatg ccaaaatgta tggacatgat ttagttataa ttgatacggc tggtcgtttg
    18301 gcaattgacg aagaaatgat ggatgaaata gaaaatatta aaaaagctgt taatcctcaa
    18361 gaaattcttt ttgtagttga tgcaatgact ggacaagatg cagttaatac agctaaagaa
    18421 tttaacgaca gacttgattt tgatggtgta attttaacaa aattagatgg tgatacacgt
    18481 ggtggagcag ctctttcaat tcgctctgtt gtcaataaac ctattaaatt tgttggtact
    18541 ggcgaaaaaa tggaagctat tgacgtattt catcccgaac gtatggctga ccgcatattg
    18601 ggaatgggtg atatagtttc tttagttgaa aaagcacaag aacaatttga tgaagaggaa
    18661 tctcgcaaac tacaaaagaa aattgctaaa gaccaatttg actttgaaga ctttttagct
    18721 caaattaagc aaattaaaaa aatggggaac ataaaagacc tcgcgagcat gattcctgga
    18781 cttggaaaag caattaaaaa tttagacatt gacgatgacg cttttaaagg aattgaagca
    18841 ataatttact ccatgacttt agaagaacgc agaaacccta atattataaa cggaagcaga
    18901 agaaaaagaa ttgcagacgg ctctggaact actgttgttg aagtaaatag aatattaaag
    18961 caatttgatg agacaaaaaa aatgatgaga gcactttcac ctaataaaac taagaaaaaa
    19021 atggcaagaa gaatgagata gtgtaaaaac aaataagtaa ataacaagtg taaatcaaac
    19081 tactatggaa ctaattgacg gaaataaagt tgcaaaggaa ataaaagaag aaataaaaat
    19141 caaagctttg caaatcaaac aaaaatgtgg tcgtgctcca catcttgctg ctatattagt
    19201 tggtaatgat ccaggctctg aatcttatgt taactataaa atgaaagatt gtgaagaagt
    19261 tggctttgag tctagtttaa tacgattaga agaaaatgct gctgaagaag aactttacaa
    19321 aattattgac aaattaaatt cagacccttt aattgatgga tttattgttc aactaccact
    19381 acccaaacag tttgacagtc aaaaagtaat agaattaatt gatccacaaa aagatgtgga
    19441 cggttttcat ccaatcaatg ttggaaaaat gacaatagat ttagattctt ttaaatctgc
    19501 aactccaatg ggcatcacta ttcttctaaa taaatatgga atagaaactt ctggaaagca
    19561 ttgtgttatt attggacgta gtaatattgt aggtcgtcca cttagtattt taatgtcgca
    19621 aaaagctatg aatagcactg ttactgttgt gcatagtaaa acaaaaaata ttgcagaaat
    19681 ctgtcgccat gcagatattt taatgactgc tatggggagt ccaaagtttg ttacagccga
    19741 tatggttaaa gaaggtgctg tcgtaattga tgttggaaca actatgattg aatctaataa
    19801 aacaaaaagt ggatataaac ttactggtga tgttgatttt gaaaatgttg ccgaaaaatg
    19861 ttcatatatt actccagtac ctggtggtgt tggtccaatg acaagagtag ctttattact
    19921 taacacttta aaagctgcag aacaaaatat taaaaaatag tttcaaaacc atcgtaaaca
    19981 atatacaaat aaacttgtta attttgttta gataaaacta taacaaatac atgcacaaca
    20041 aaatagcaga tattcttcaa aaatccgttt taagtaaaaa tgatttaata attttattat
    20101 cagcaaataa agataatgca gaaataattt ttaataaagc tgcagaaatt aaaactaaat
    20161 atattggtaa ctacacctac tttcgtggac taatcgaact atcaaatatt tgcactagaa
    20221 attgcttgta ttgcggaatt agaagtggca ataaaaatgc tgagagatat atgattagtg
    20281 atgaagaagt tttcaattct gtaaaatatg ctttagaccg tcattggggt tctgtagtta
    20341 ttcaatcagg tgaaagaagt gatactgctt ttattgataa agttgagcat attttaagac
    20401 aaattaccca aatgtgtgag aaaaaaccag ggattacact ttctctcggt gaacaaaaaa
    20461 ctgacacatt taaacgttgg tttgatgctg gtgccacacg atatttatta agaattgaaa
    20521 gctcaaatcc tgacattttt aaaaaaattc atccacagga taaacttcat aatttagaaa
    20581 gaagaataga aaatctttat acactacaaa aactaggtta tatcacagga acaggtgtaa
    20641 tgattggttt acctttccaa actattgaag atttagccaa tgatttgtta tttatgcaaa
    20701 aactcgacat tgatatgatt ggaatgggac cttacatcga acatgaagac acgccacttt
    20761 atcaatatcg tgatctatta cttccaattg aagaaagata ttttttaagt ttaaaaatga
    20821 ttgcagtttt acgtatttta atgaaagatg taaatattgc ttcatcgaca gcacttcaag
    20881 ccataaatcc agacggcaga gaagaaggaa ttagagttgg tgcaaatgta attatgccaa
    20941 atattactcc tgtacgctat cacaaaagtt ataatcttta taaaggcaaa cctgacattg
    21001 cacctgaaac agataattat atcaaaattt tagaaaaaca aattgcttta gctggcgaca
    21061 aagtgggcta tggcgacaaa ggcgatccaa aacatttttt taggagaact gtagaaagat
    21121 aagttttata agtttatttt tatttgtctt attgcattta tactgcacaa ttactaaaaa
    21181 aattaaactc caaaagcatt tgttgaatta tttttatcta tctggctcca taccaaacca
    21241 cacaacagca tattcttcat aaattgcaat tccaattgcc ttccattcgt tatcagacca
    21301 gttttcttta ttgataatca tatttttatg agctgggctt tttttccaag catttaaagc
    21361 agaacttgct gttggtccag aaggattcca atgagctatc tcaaaaccat aatgtttata
    21421 atttgtcaat tctcccggct tgctccacac acaatctgca tttgcatgat ctggagtata
    21481 acaacaagca gtccaattac ctttatttga ccatgaatgc aaattacacc tatcattatg
    21541 tttataatta ttctttaaat caatggcatg tgtatgagca acaaagttta acgaacgtga
    21601 aattggaatt ctaggcaaat caaattgctt acgataagcc attatcaaat cataaagcac
    21661 tttttcctct gcggtgtttt catataaatt ttgattttga gaaaaagtaa aactattcac
    21721 aaaaattaaa acacttaaca aaaataaatt tttagttttc ataatctcag gtttctttaa
    21781 taatataacg tcctataact acaatttatt ctatctgttt taaaaaacca tttccacgcc
    21841 actcacaatt attccccatc taataaggaa tcaacttcac cctaacttca tctccttttt
    21901 tcaaatttag gtttaaaaat ggagtatctt ttccctctat ttttttatta ttcaatttta
    21961 ctgttttaac atagtcgggg attttgattt tatattgatg atctgctttt gctctgagcg
    22021 taaactcttt tacctgatga ttttcccaaa caaaatcgat ttctcctgct ccacgaacac
    22081 aaactccttt gatactaccg ttcttccatt tatcgggcaa tgcaggaagt agctcaataa
    22141 aaccatgctg actctgaatt aacatttcag caattccagc acttccgccc aaattaccat
    22201 cgatctggaa aggtggatga gcgcaaaaaa ggttaggata agttcctgct ctttgctctt
    22261 cactattttc aggattccaa gctggttcga gcagtttttt caacaaacta taagctcggt
    22321 ttccatcttt caatctagcc caaaagttaa ttttccaagc tcttgaccac cctgttcccg
    22381 catcacctcg tctattgagt gttacttttg cagcctgagc caattctgga gtaaaatcag
    22441 gagaaatttg atttgacggg tgcaatccat acaaatgaga tacatgtcgg tgatgaatat
    22501 caacttcttc ataatcttcc aaccattcca ttaaatatcc ttttgggctt attctatttg
    22561 gtggaagacg taataatgcc tgctcaacag cttccgtaat attatcattg atattcaata
    22621 ttttagctgc cgaaagaaga ttattaaaaa gttcacgaat aatctgtacg tccatggtcg
    22681 gaccatgaca aacatagatt gtcttatctg aatttggaat tttaaaacca ttctctggag
    22741 aagaagaagg agctgttacc aaataatgat attgaggttc ttctatcata gatgaaagaa
    22801 aaaattgagc tgcaccacga ataataggat atacagatcg taaaaaagct gtgtccaatc
    22861 catattgata atgctcccaa agatgcgaac aaagccaagc tccaccagta ttagttgctc
    22921 cccaagaagc atgctcaccc ggagctgtaa aataccatgg atttgtcatc atgtgagcca
    22981 cccaaccatc tgcattataa aatccttgtg ctgttttctc tcctgaagct tgcatcgact
    23041 cagtcaatct aaccaaaggc aggtgaagtt cagccaaatt tcctacttca gccaaccaat
    23101 aattcatctg tagattgata ttcaaatggt aatctccatt ccatggtgtt tgcacttgat
    23161 tagtccaaag tccttgtaaa ttaagtggca aactattttc tctagtacca ctaatcatca
    23221 agtagcgacc atactgaaaa taaagtgcaa ccaaagatgg atcatcatct aattgaaatt
    23281 gaagtaagcg ttgatctgtt gagacatcaa gtttttgaac tcctaaatcc agcttaactc
    23341 tatcaaattt ttcacgataa acttcaatat gacgcttttt caactgctca aagctatatt
    23401 tttcagcatt gctcaagttg tcttgaaccg tttgaatata attcttatcc aacatattgg
    23461 ttgaagtact catcaatagc gttacctcag aagcattgct aataatcaaa gaagtatctg
    23521 tagtgctatg cattcctcca tttttttgaa tttttaaagt aaaaaaataa cgaactcctt
    23581 tatcgccatc ataaccgtca tttaattgtc cctgcattac aatttcatca gcatagacat
    23641 caattttcac tctttcggga cgctctaaca ttatctccat cgacaattca cctttattat
    23701 cagtggtgta atgagcgacc aacaaatcat ctgcgtgaga agcgaaatat tgtcttttgt
    23761 atgtaaattt tcctttttta aattgcgtag tggcaacggc atcatttaaa gagagcttac
    23821 gactatactc tgtaacttca gatttattgg gataaaaatg tttaactttc agtttaccaa
    23881 gcagttgaaa agaaccgtaa ggagcatctt taccatttcc aaaattagaa ccttttccac
    23941 cacatttaaa atagtgatac accaattctt gagcctgctc attttttcct tcaagcaaaa
    24001 gctgacgaat caaaggaagc tgttcttttg cattggggtt tatagtttcc aaatctacac
    24061 ttcccgacca cattgtaatg tcgtttaaaa tcacctcctc catatcaata gctccatcta
    24121 tcatcatgcc tattcttcca ttccctagag gaagcgtttc ctcccaacaa gtggctggct
    24181 tatcaaagta caagtgcaaa acctgcttgg attctttact tatcgccttt ccagaaaaga
    24241 tgaaaagaaa aaaatgagta gccaatatca atggtaaatg tttcatctta aagagataat
    24301 atgtgcaaat ataagtttat tttttcatct gtgattaaca cataataaat attttaaatt
    24361 tacacaagtg gtattttaaa tttacacaag tggtatttta aatttacaca atgaatatct
    24421 taattttaca cgaataatat tttaatatta cataacgaat attttaaatt ttcacaacaa
    24481 ataaaacttt atttttacat atatttttaa agtatagctt tggatttgca tagattttaa
    24541 acataaaata ttatataaca aatgtgttaa cttttttagg ataagaatgt tatactttac
    24601 taaaactgtt aatacataac caattaccaa atccttttca tcaaacactc acctcattcc
    24661 aaggcaccac ctgagccaca cttctttcct gacgttcgct gcctccataa attagtgttt
    24721 ttgtcagttt tatttctgtt tcaagtgctg cataataatt taatccctta aataaatcag
    24781 gcataacagt ttgtgtagct tttatttcta caatttctgt ttcttgtggt ttttctaaaa
    24841 gtaaatccac ctcattacca tgagaatcac gccaaaacca caaatctgtt tgtctcgcat
    24901 tttgatgata tttctttttc acatactccg aaactatcat attttcaaat aaagcacctt
    24961 ttacagcttg attttttatt ttagacacat tatttattct gagcaaatta cagagcaacc
    25021 cagtatcata aaaataaagt ttaggcgatt ttgtaatacg tttgctaaag tttttataat
    25081 aaggttgcaa cagaaaaaca acatagctat tttccaaagc agacaaccat gcttttacag
    25141 ttggatccga aattccactt tcctttgcca aactactcat atttaaaatt tgtcctgctc
    25201 ttgtagcgca taaagataaa aactttttaa acaaactcat atcttgaata gcgagcaact
    25261 cacttacatc tcgctgaaca taagtttcaa tataattaga ataaaaatct gctggtttaa
    25321 tttttcgatt ataaatcgca ggataaaatc cctttagtaa atgtgttgta tagtcatttt
    25381 gtagcaatcc tgctcttttt aattctttaa aatcaaaagg aaataatcta aaaatagcaa
    25441 ctctacctgc caaactttgt gtaatgtttt gcatcaaatg aaaattttgc gagccagaaa
    25501 aaataaattg ccccattatt ccgctgtcat caacaataga ttgtatatat gaaaacagat
    25561 gcggtactcg ttgtatttcg tcgaaaataa tatatttatc atattgtttc aaaaaaccac
    25621 gtggatcact ttctgcaaaa atcctcatat ctgggtcttc aagactaaca tatctatagt
    25681 cataaaacat tgctttcaac aaagtcgttt ttcccgattg ccttggtcct gtaagaacaa
    25741 caactggaaa cttatctcct aactgtcgca ttgcagtaac tatttctcga tgaactacca
    25801 taattatttt ttaatgtatt tataatgcaa atataaataa ttattttaaa ttttcacaat
    25861 atttttctta atattacaca aagactattt taaatataca caacctacat tttaatatta
    25921 cacaactatt attttaaaat cacacaatac aaatcttaaa tttacacaat tattatttta
    25981 aaactacaca agcattattt taaatataca cgaacaatat cttagttttg cacaagattt
    26041 attttaattt tacacaaata ctattttaaa tatacacaaa taatatttta atattacatg
    26101 aaacatattt taaaattaca caataaatat cttaagattt cacaaatatt tttcaaattt
    26161 acacaacaaa catattaaaa ttacacaagg aatatcttaa atttacacaa acaatatttt
    26221 aaatttatcg aaaagtatat ttttaaaaga aaacaccatt ccacaacatt gagaggaact
    26281 ctagggcagg ataaacttcc acgttgttta atctgcgagc atatttatct aacgaaacaa
    26341 caataagtcg gcaattggga aattcttctg aaaaagcctt caaacctttt gtatgatgtg
    26401 attgcacttc atctgaagat ttaatttcta tggcaacttc ggcatttccg ataattacat
    26461 caacttcgta acccgaagat gtacgccaat atgacaaatt ttgcaaaggt ctgaaatatc
    26521 cgatataagc aataatttcc tgcataataa aatgctcaaa agcatgtcca aactctggtg
    26581 agccaggatt taccgaagtt cttttaagca gaaaattagc aatacctaca tcaaagtagt
    26641 aaaatttagg cgactgaata acacgccgtt tcacatttcg agtaaaagct ggaataatat
    26701 aagctataag cgtttcttcc aaaatagaaa aatactcttt aactgtagga gcactaacac
    26761 cacattccaa agaaatatta ttataattca aaatttctcc attacttagt gctgcaactt
    26821 ccaaaaatcg agtaaaggta tttagatttc gagtaagcgc ttcagctttt atttcttgtt
    26881 gcagataatc acccacataa gcctgaagtc tgtttgtagc attttctact aaatagtgtg
    26941 gtggaagcat tccattatta caagctttca caatatcaaa ttctggaatt tcaacactaa
    27001 caaacggata taggtgtttg cgaatagctc taccaccaag caagttcaca ccacttcggc
    27061 gtaatttgcg agcacttgaa ccactcaaaa taaagcgaag atgatgatta cttattaacc
    27121 aatgcacttc atcaagtaaa gcaggtattt tctgaatttc gtcaataatt attaattcat
    27181 tcttgggaag taaacttaac tcttcacgta aaagagcagg acgacggcta taacgtgcaa
    27241 attcatctga ttttaataaa tcaatataac gaacaccggg aaaccgcatt ttcagtaacg
    27301 tacttttccc tgtttgacga gcaccccaaa gaaaaatact attggtttca acatcttgga
    27361 aattaaattt tcgcaataac atatctaagt gttattaatt taagttacac aatgcaaata
    27421 tataatattt ttatgtaaat ctaaaatata actttatttt tacatgtatt tctaaagtac
    27481 aacttttgat ttgcatagat tgttgacgca aaaaatgtca attttttagg acataaaaac
    27541 tctaaatcac ttttaatatc tcgtttttag caaattctaa tgcttgagaa attccatttg
    27601 gattttttcc acctgcagtt gcaacaaatg cttgtcctcc accaccacct tggataaatt
    27661 ttgctgcatc acgaattaaa cttcctgcat ttaaagattt ttctttagtt aaattttcag
    27721 aaaacgcaat tattaaagat ggtttatctt ctatagctgt tgctaataca caagcaaatt
    27781 gagatacttc tcctctaatt tgtgaacaaa tttctttcaa atcattagca tcttttaagt
    27841 ctatttccga aacaataagt ttcacatctc caacattttc agcttgctct aataattgac
    27901 gtttcactac ttttaattct tctgcatgga atttttcaaa ttgttttttc aatttttcat
    27961 tttcttccaa aacattattt attgctttta gcaaatcttt ttgattattg aaaattaaag
    28021 ctatatctga aactgtatca aatttttcga aaactaaatt ttcagcaact tcgcctgtaa
    28081 ctgcttctac tcttctaatg ccagcagcaa tagcagattc agaaacaatt ttaaaaaatc
    28141 ctatttgacc tgtgctttca acatgagtac caccacaaag ttcaacggaa tcgccatatt
    28201 gtataactct gacttcatct ccatattttt cgccaaacaa tgcaacagct cccatttctt
    28261 tagccttttt tataggaata tttctttttt cgtttttgct aatattttga cgaatatttt
    28321 tattcacttg tctttcaatc aagcgaattt cttcatcact tagtttattg aaatgtgcaa
    28381 aatcaaaacg caatctttct ggtgaaacca aagagccttt ttgctctaca tgacttccta
    28441 aaacaccccg caaagcatga tgtagcaagt gagtagctgt atgattattt gcagtagaag
    28501 ttctattttc aacatttact tcagcggtaa aagttgcttc tggattttgt ggtaaatatt
    28561 ttgaaatatg aattactaaa ttatgttctt ttacagtatt aataatttca atattttcgt
    28621 ttatactacg aataaaaccg ctatcaccaa cttgtccacc tgcttcagca taaaatggag
    28681 ttttatcaaa aataagatga taatatttac tatttttttg ttctactcgt ctgtatttta
    28741 cgatatttat ttctgctttc aagttatcgt aacccacaaa ttctgtttct tcaatatttt
    28801 tcaaaactgt ccaatcgtca gtatctattt ctgcagcagc tctggctctt tctttttgct
    28861 tttgcatttc gccatcaaaa ccctcaacat caacgacaaa accattttca cgagcgatta
    28921 gttcagttaa atcaagagga aatccataag tatcatacaa aataaaagcc tgagttccgt
    28981 taacagtatt tttattttct tttttcaatc tttccatttc agtatctaac aagcgaatac
    29041 ctttttccaa agtacgtaaa aacacagttt cttcctcttt aataactttt tcaataatac
    29101 ttttctgact aataagttct ggaaaagagt ctcccattaa atcaacaaga gtatcaacta
    29161 atttataaat aaaagcttct tttaaatcta agaacgaata agcatatcgt acagcacgtc
    29221 ttaaaattcg cctaataaca tagccagctt tattatttga aggaagttgt ccatcagcaa
    29281 tagcaaaagc aacagcacgt aagtggtcgg caattacacg taatgcaatg tcgatttttt
    29341 catcttctcc atattttttg ccagaaattt cagaaatatg atttattaaa atttggaaaa
    29401 tatctatatc ataatttgat tttacgcctt gcaatacagc acacaaacgt tcaaatccca
    29461 tgcctgtatc cacatgtttt tgtggtaaaa cttctaaact tccgtctgct tttctattat
    29521 attgaataaa taccaaattc caaatttcaa taacttgcgg atggtcttta tttactaaat
    29581 ctttcccgtc aatttttttg cgttcttcat cgcttctaat atcaacatga atttccgaac
    29641 atggaccgca tggacccata tctcccattt cccaaaaatt atctttttta caaccacgta
    29701 caattctatc ttctggaaca tattctttcc aaatttcttc agcttctgtg tctatttcta
    29761 aattttcggc ttcatcacca ccaaaaacac tagcgtaaag acgagattta tcaattttat
    29821 aaacttctgt taataattcc caagcgaaag caatggcttc ttttttaaaa taatcaccaa
    29881 aagaccaatt tcccaacatt tcaaacatag tatgatgata ggtatcgtgt cctacatctt
    29941 ccaaatcgtt atgtttacca gaaactctga ggcatttttg tgaattagca actcttaaac
    30001 tttcaggttt tcgtgaacca agaaaaatat ctttaaactg gttcatacca gcatttgtaa
    30061 acattaaact tggatcgttc tttattatca tagaatcact agccactatc ttatgagatt
    30121 tttcctcgaa aaaatcttta aatttttgtc taatttgttt agaatccatc atatacaaaa
    30181 tcaattatca agttattaaa aactttattt tattttagtt tgcaaagtta gttaaattat
    30241 gtaattttgc actttatttt agtattaaat atttttaatg acaaaaatta aaacaaaata
    30301 taaatttaat tcagaaacgc ttcaatacga aaagattaag ttcagtttta aaaagttttt
    30361 ccttaaactt tttcaacgtt ctgttgtatc tttttcattt ggtgctattt taatggtatt
    30421 atatattata atttatgagt ctccagaaga gaaactctta cgagctgaag ttaaattttt
    30481 gcaagaaaat ctaaataaaa ttaatcaacg tttaaaagta aacgacagct tattaaatgt
    30541 gattgctaaa aacgacaatt atatttatcg caccactttt gaattagaca ctattcctac
    30601 tagtgttcgc aatgctggag ttggaggcag taatagatat aaaatttatg aaggttataa
    30661 tagcactgaa attataaaag atgttgctaa acaattagac gaatttgagt tgaaattaaa
    30721 aattcaagac gagtcgtata aaattttact agctgaaatg aaaaagcgag gtaatattta
    30781 taaatctctt cctgttttac aaccaattca cgtaaacgaa ctaactcgta taggttcttt
    30841 ttatggttat cgtcctcatc caattttagg ggtaatacaa atgcatcatg gaattgatat
    30901 ggttgctcct actggcactc ctgtttatgc ttcgggcgat ggtgttgttg aacttgttga
    30961 aaaaattaga actggttatg gtaataatat agtaattaac catggggtta atggtctaaa
    31021 atcaagatat gctcatttac atactataaa tgtaaaaaaa gggcaaaaag ttaaacgagg
    31081 cgaacttatc ggaactgttg gttcaactgg tttatcaact gcaccacatt tgcattacga
    31141 aattttagtt aacaattcaa gtgtcaaccc acttaggtat atgcttgttc caaacccctc
    31201 agaatatgaa caattaataa atgaaactca atatccgggt aaatcttttg attagtttaa
    31261 tagtaatagc tcttattttt ttttgtagct ttagttttct taaacttcca ccaaaacctg
    31321 atgatgatgg tataaaactt ataataccaa catttttagg aaacgaagaa cgcaattact
    31381 acggaaatat agctcccgaa agtttagata ttatatggaa aactacatta ggttgtggaa
    31441 aaactgtttt tcctaataaa gatcaagaag ttagagatat gtgtggtgcc ggctggactg
    31501 gtcaagcctt gcttatagaa gaccaaggcg agctttatct aattcaaggt gcttacgatt
    31561 ataatttaag aaaaattaaa gctaaaacag gcgaagttgt ttggaaatat ccttatgacg
    31621 atgttataaa atcgactggt tgtgtttggg ttaatccaaa tagaactaaa gatgaccctg
    31681 ataaaatagt gatttttcaa ggcagcagaa gaggatttaa agttccactt cacgaaaagt
    31741 atatttttag ttacagagcg atttcgttta gaacaggaaa agaattatgg cgatataatt
    31801 ctgtatatgg tggctcattt agcagagatg tggacggaac agcattaatt gttgacgata
    31861 caattttttt aggattggaa actggtgatt ttgtaaaact caacccaaat ccagattctg
    31921 cacaaataat tgattcttac aaacaacctg tagaattttt taagcaagca ctttatacaa
    31981 aagaagatat tcaagctcat cgtagaaatg tcgtaattga aagcgctcct tcattattag
    32041 acaattttgt ctttataacc tctggtgctg gatatttata taaatatgac aggaatagcg
    32101 gacaaattga tattcaattt aaaactggct cagacatgga cggatcgcca attatcacaa
    32161 gagagaaaaa agttttaata agcattgaaa aggaatatat tcctggcaaa ggtggagcaa
    32221 tgctaattga cccaacaaaa cataaagact cttgtgttgt ttggttctac cctaccggag
    32281 acaaacactt tgtaagttgg tatggcggga ttataggctc gtgtgctatt aatgataggt
    32341 atatttctga aaatgaaaac tcattagctg cttttgttgg aattgacggt tatttgtatg
    32401 ttgttgaaaa tgaacttact aacccctacg atttggtaaa aggaccctgt gataaaactt
    32461 tatatcctca acctcaagaa attttcaaat atgaaacagg accttcaatt tcaactccaa
    32521 tttttacaaa agacagactt atagtcttga cttacgaagg cttgtacatg tttggatatg
    32581 ataaaaattg taattttagt ttattagaga aaaacaccaa tattagaggc gaagcaactc
    32641 caataattca ttacggaaga ctttttgttg cttcaagaga tggaaattta tattgcctgg
    32701 ggaaagtaat taagaattga gaattaagaa ttaagaattg tatctcgact tcgctcgaca
    32761 ccccgacttc gctcgacacc ccgacttcgc tcgacactca ataattgaaa tgagaattaa
    32821 gaattaatgt ttctaacacc acccttcttt gcaaaggctt ttgcaatata ataaaatgct
    32881 aatccgatta acaaagttgc cagtccaatt aaagtttcta aagttttttc tttaaacata
    32941 aaaatcatat tccatattac aaaaagtata aatattattg gaacaattgg atagccttta
    33001 gttttaaaag gtctggacat atttgggtat tttcttctat gaataaatac tcctattact
    33061 gttaaaacag caaaaatatt catcacaaaa cccaaataag acaataattg ttcaaaagtt
    33121 gatgtaaata ataaaatcaa acttattgaa ctttgaatta aaatagctac taaaggcact
    33181 ccatttttgc gttttctttt tataaaactc aaagctggat aatcttctcc aattacttga
    33241 attactcgtg gtccagcgaa aaccatagaa gaaatagtag aaattaataa cagagaaatt
    33301 aatcccgaca taatcattcc gccttttgca ccaaatatat cgccagccac aattgcacca
    33361 atatctaatt gtccagttaa agaacttact ggtgatacat ataaaaatat gaaatttacc
    33421 aataaatata aaacagtaac tattaaagtc cctataatca aggatcttgg taaatttttt
    33481 gctggatttt taagttccga aacaaaatat gctgatgcat tccaacccga ataagaataa
    33541 tacacaaaaa ttaatgatac tgcaaagcct gagcttaaaa cttgagacca agttacagaa
    33601 cttggtataa tggaaacatt ttgcggttct ggtgtaaata caaaaccagc aacgataaaa
    33661 gcgataatca ataaaatttt aattattgtg aaaatatttt gaaatgcacc acctgatttt
    33721 aaactagtag aatgtacaat agtaactaaa actataactc ctgctgcaat atattgggga
    33781 ttgattcctg cataaacatt agaaaaataa gtacctaaag ccattgaagc caatgctatt
    33841 ggtgcagcga atcccacaaa cattgaaaaa aaaccagaca taaatcccaa agcagggtga
    33901 tataattctg aaagaaaatg atattcaccg ccagaacgag gcattactga gcccaattcg
    33961 ccataaactt gagcaccagt aaaagcaata attccaccca ttagccacaa aatcattaca
    34021 gcagccacat ctgtaactcc accagcaagc tgaaatccaa gagaagtaaa cactcctgtt
    34081 ccaaccatat tcgcaatcac ataagaaatt gcagtcagta aacctatttt ttgtacagtt
    34141 tttgaatttt tcacaatatt ttatttattt tggcacaata ataatacaat tcggtttaat
    34201 tttaaaatca taaaataaat ttaatttaat gaaaaagatt attcccctga ttatccttac
    34261 attagcaatg tgttctgcat gtaataatct taaagaaaaa aaaactgttg aaaacgcaaa
    34321 aacaacagaa agtcaagaaa taactaaaaa tcaagaagtt gtacaaattc ctcataaaaa
    34381 cgacactatt ctttcatata aatatgactg ttgttttatt gttagcacaa aaataattta
    34441 tcctccaaaa gatgctaata ttaaaggtgc aattttatta ttacatggtt ggaatttacc
    34501 tgctgacgaa tggtgcgaaa aaaccagctt ttgcgaaaaa gctttaaatc aaaattatgt
    34561 tttaataatt cctgattaca gcaaatcaaa ttatacttta gaaatttatc cacaaactat
    34621 tgcaaattat aaaaaatatc caacaatcac atggattatg gaaacacaaa ttgctatgat
    34681 acaagaaagt atgaacttgc tgttgccagg tcaaaacacc tttgttgcag gaattagcac
    34741 aggtggtaga ggtgctacac ttcttgcgta ttatatgcca aatattttta aagcagttgc
    34801 aagcctttct ggtgattttg atattacaac aatgacagat gaatatatat attattcatt
    34861 tttaggtcat tacgaagatt ttcctgaaag atggaaaaaa gaatgctttg cttatgattg
    34921 caaaaatttt aaaactccaa tttatattgc acatggcgag gctgatagag tatcgcctgt
    34981 tgagcaaagc aaagcaatgt atgactcaat taaattacat caaccaaatt taaaaattaa
    35041 agcaaatttc cctactgcag caggacataa ttacgacttt tgggaatatg aaacagataa
    35101 tgttttgaaa ttttttgaag aagtttacca agaattacct aaataattgt tttaacaata
    35161 attttttaag ggcttttata aaacattaaa caaaaaacat ttaacattct ttaattagaa
    35221 taattttgta taaatttacg caaaattatt ttatgaaaac taaactttta ctttttgcat
    35281 taattttgtg ttcaagtttt gtttttggac aaaataaaga ctttgaatca cctgcaaaaa
    35341 cttcaaaaca atcaataatt ttgtttaaaa gtttagttta cgattttgga aaaattaaac
    35401 atgaatccga agctaaagct gtttttgttt ttaaaaaagt aagcaaaaag cctgtaaaaa
    35461 ttactaatgt acaaacaagt tgtggttgtg ctgctgctga ctggtctcga gaagaaatta
    35521 caaataagaa aaaacaaaaa atttcaattg tttatgattc tcgaataatt ggaaaattta
    35581 caaaatatgc ttatgtttat gtcgaaggac aagatgaacc tatacaactt caaattaaag
    35641 gtgaagtttt gccttcagac acacaagaac aagctataga aaataaaaca aatgtttcta
    35701 aaaaagaaaa caagacaaaa agtatgaatc aaaaaaagaa aaaaattaat aataactaat
    35761 atatttaaaa gattatgcca aaaatttcta aaaaaggtat ggcaatgcca tcttcaccta
    35821 ttcgcaaatt agttccttac gcagaagcag caaaagctcg tggcgttaaa gtttaccact
    35881 taaatattgg tcagcctgac attaaaactc ctgaggttgc tttaaatgca gttaaaggaa
    35941 tgaaagatac tgtaattgct tacagtcact ctgctggtaa tatttcttat cgcaaaaaat
    36001 tagttgaaca ttacaaaaaa atcggtcttg aacttacacc cgaagacatt attgttacca
    36061 acggtggttc tgaagctatt acaatttcgt ttatgtcaac tttaaacgaa ggcgatgaag
    36121 ttattattcc agaaccattt tatgcaaact ataacggttt tgctattcaa acaggagtag
    36181 ttattaaacc tattacatca agtattaaga acgattttgg acttcctgca cttgaagagt
    36241 ttgaaaaact tatcactcca aaaaccaaag gtattgtaat ttgcaatcct aacaacccaa
    36301 caggatattt atattcaaaa gaagaactgt accaacttgc tgaaatagtt aaaaaacacg
    36361 atttattcct ttatgctgac gaagtttata gagagttttg ttatgacggc aacactcact
    36421 tgtcggtttt aaatattcct ggcttagaac aacatgcaat tatgttagat tctgtttcta
    36481 aacgttatag catgtgtggt gtgagagttg gttggatagt ttcaaaaaat aaagaagtga
    36541 tggcaactgc tatgaaattt gctcaagcaa gactttgtcc tccatcatta ggacaaattg
    36601 ctgccgaagc tgctatcgac acaccagatt catatttcga agaagtttat aacgaatata
    36661 ttgctcgtag aaactttatg gttgcagaac ttaacaaaat acctggcgtt tattgtccaa
    36721 acccaaaagg agctttctat acagttgtaa aacttcctat tgatgatgct gataaatttg
    36781 ctcaatggct gttagaagat tttgaatata acaaacaaac tgttatgctt gctcctgcca
    36841 caggttttta caatactcct ggacttggca aaaatgaagt tagaattgct tatgttttaa
    36901 aaatcgaaga tattaaaaac gcaatagaaa ctttaagaaa agctcttgag gtttacccag
    36961 gaagagttta aagcgatgca ctgaacgtag ttgaagtgtt aaaaattttt aaagaaaatt
    37021 gcataaaaat agagatattg cacacaatgt ctctattttt ttacttaaac acaaaagaat
    37081 ttggaataat tcccaccgca aaactatcaa cacaatgtaa atttgagttg taacgataaa
    37141 ttactccaga ttgcatatag tcaactgcat cggaaatata aaagtcgtaa ttataagggt
    37201 cttgtcctat gccataaata gttttatgtc ctacatttat ccattctttt tgaggtaagc
    37261 tagttgcatt tattggaaat ctaaaaatat tgccatttac ataaaaaatt gtatctccag
    37321 ttttgttgat ttgcatatct gtaatattgt ctttgcttgg aagaacaaaa ctttgctcaa
    37381 ttttatgagt ttgcatattt atttttgtaa ttccagcatc gcctttataa tcactgtaat
    37441 cgcttccgcc atcgcttaaa acccatagat ttttttcttt gtcagcaaca atttttcttg
    37501 gttgtttaaa gacttcaatg ctgtcaatta cttcatctga attagtatct ataactaaaa
    37561 ttttattgtc gtaagaccaa gtatttataa ataatttatt ttcaaaaacc acaatttgct
    37621 ccgatgaatg attatagtat tttccacttc cgtcatcaat atttattgtt tttgtcaaac
    37681 tataatcttg aggatttacc acataaatat tttttgcata caaatcagta atataagcct
    37741 tttgaggatt tataaatctc atatatcttg gcgaaactaa gtctgtaatt tttcctttaa
    37801 ttttaaaatt atcaatatct attgcataaa cttttccgct attatttaca attatccacg
    37861 cagttttatc ataaattata agactttgag ccacatcgcc aagcggcatt ccatttgcac
    37921 gaataaaaac tgtattttca acttttttct ctaaaggcga ataatatgaa agcgaggcat
    37981 ttccatacat aaaattccct tcacaaacaa taaatactcc tcttgaattt gtatcaaact
    38041 ctgttattgg acgaaaatca tctttcatac aagaagttat gaaaatgcca aaaacaaaca
    38101 aacacaaaga caaaattcta tatattattc ttctaatttt cattttaatt ttatacctaa
    38161 ctgtaaacta taattccgtc ctggcatagg ttgccacaaa accgacctat atttttcgtt
    38221 aaataaatta tatattttaa aattaagaac taaatcaact tttttgaaaa taaaattttt
    38281 gccgatacca atatcattca taaaatatgg ataaatactt accaaaactc ccggctcagc
    38341 aactgaactt gtgtagcgag tgctaaaata tgtccattga taattaaaga aaaagtttga
    38401 ccacgaaacc tgagaaaata aatttgcaga atgtcgtggt atataaggca actgcttacc
    38461 taaagattgg tcgtttggat ttgcattttc gtctgctcta accgattttg taaaagcata
    38521 atttacaata tttttaaatt ttacttttcg caatttatat tcaaaaatta aatttgtttc
    38581 gcaacctgaa gcaattacag catcaatatt atcgggtcgc caaaagccca tagcagttgg
    38641 tcgccacaat atccaattat ttattttatt gtaagtaagt tcaaaatcgg tttttaaaga
    38701 aaaattcttt ttatttaaaa aataagaaac tccaagattt gaattatttc caatctctgc
    38761 ttttaaatct ggatttccac ttggtttgag atataaatcg tttaaacttg gcaaattata
    38821 atttcttcca acagatgctc gcaaactcaa attattttta aaaaagtact ctgccgaaag
    38881 cacagggaca aatggattta agtttttatc acaaaaatct tgtcgcaata aaagagctaa
    38941 tttaaaattt ttaaaaattt cataatatgc agaaacatta agtccaccct cttgtctttt
    39001 tgctgtaaga tactgattta caatactatc ttctgaattt acaaagtgaa aattataatt
    39061 aatttgtgca tttagctctg ctttttcatt aaatttataa tttgcagaag ctgaattata
    39121 aattgaattt gattttgaaa aagaattaac aacattcaaa taaccaacac cattaatata
    39181 gtttgtactt ttataattca aattttgaaa atttgcacca tgattaagct caaatttaag
    39241 tctttctcca taatgagtat agtttgttgc atagacattc attccagaat tttgacgatt
    39301 tttgttatta ttaggtccag cttcgttgct tgttaagcct ggtacacccc tatccgacaa
    39361 ctgtttccag aattttgcag aaataatatt tttatgatta aatctaaaat aaacttcctg
    39421 aacaaaacca tattttaaat aatctgcatt ttggcgatat tcaatttgtt gattagtaat
    39481 atcgttatta aaaaatggaa aattattttt tgaaattgaa tgatataact ttgtaacaga
    39541 ctgaacctta gcgtttccaa gtctaatttc gcccaaatta tcataagaag aataagatgc
    39601 aacttgactt acaattttag ctttcactcc tttagaccaa gttgttgaag aatttagctc
    39661 tatcaagcca cccaaagcat ttgaattttt gcttaaagat gcagccccat aataaatttt
    39721 aatattttcg ctgatagcca caggaattag cgacatatcc acaattccca ttaaaggtga
    39781 atttaactca acattattcc atgtaagttt tgtatgagat gcatttgtcc ctttaaacga
    39841 agccgttgat agagatgact ttccataaga ttttataaaa acacttgtat tttcagaaag
    39901 aagcgatgaa atatcgtcta aaaatttatt ttctatgatt agactatcta atctattaat
    39961 tttttgacca gtttcttcaa gattttttgg tgcataaata aaaacttctt caataagatt
    40021 aattgtgtcg ggaatatgaa tttcttgtga aaacaaacaa ttcccaacta acaaaattat
    40081 gataatattg agaaattttc taatcaagtt ttataatttt ttgatttaaa aaattatttc
    40141 catcaaaaat tctaataaaa tacactccag aattaagcga acttaaatcc aaaatggaat
    40201 tgctttctaa attgttctta taaattagaa ccctaccctg attatcgaaa atatacactt
    40261 cttgaatatt attttcagaa ttaattttta taaaatcttt tgttgggttt ggaaaaattt
    40321 ggaaattatt ttttgcaatt tcatttacat ttacagcaga attaattact ccaacagcat
    40381 ctaaatcaaa accgcaagaa taaaaaggtg tgggccaagg gtcgttcact atatttcctt
    40441 gagaatcgta agaagcatat tctggattta tatttcccac gacatcaata atttttacgt
    40501 gagtaatgtt ttgcacatca acatttgggt cattaatttc agctaaatca aaaggagttc
    40561 cgtaaaaagt aggaaagttc cccgcgaaat tgtgcaaaaa atctctatcc attgcatcaa
    40621 aagaaccaat ttgagtttcg aactgatatt ctgtaactgc tggaaatctt gcataatcaa
    40681 ctccattgct actaacctcg acaaagccca actctaaaaa agccattttt gtttgtcctt
    40741 gaggtgtaaa aatagcattt tcaaaaacta caaaatcagg accgtttcca tcaaagatag
    40801 gattttcaaa ttctaaaatt atatgtccac catcgcctaa agaaatagca ttaccatctg
    40861 cttttccaag tgcatcttga acttttccat gccaagcctt gtttgatgta actcctcctt
    40921 gagaataagt aaaagcagta tctgcaatgt tgatataccc acgctcaaca acagcagata
    40981 ccgcccaaga aacaatgatg gacgaatctt tatgaattgc atttgtgcct tgcggaaact
    41041 cctgtgcgaa aagcaagata ctcgaaaagc aaaaaacact aaataataaa gataatttca
    41101 ttgtttgaca gattattatt taaagatttt tgttgaatac attgaaatgc catcacttaa
    41161 acgaacgata taaattcctt gtggaaaata tgataaatca aaattcatat tattatttaa
    41221 actagaattt gaaatcaact gttttcctgt aatatcataa atttcaagag attttatttc
    41281 gttttcggac tcaatattta aaatattgtt ttgattaata taaacttttg gttcaaaagc
    41341 cttattttca gcaatattag tttccacaaa aacattaact gtttcggtta ttgtattttc
    41401 ctttgcatcg cttactgtta cagttaatgt cgtacttcca gttactgtag caactggcat
    41461 ttgacttgtg gcatcatcta aaatttctgc attagaccat tcgaagctat aaggctgaac
    41521 tccacctttt acaaaagcac ttaattgaat ttcttcgcct acacttgcat tataattagt
    41581 tttaatatct gtaataaact caggaactgt ctcaggacat gtagctgcaa aatcatctat
    41641 gcaataatag gaaggagtaa gcattccata agcattagta tctgatgatt caacataaaa
    41701 caaaagacta tcagcaacac ctgcccaagt taagtcaaca taagtccaat catcaatttt
    41761 aaattgcatt tcttcattgt cgcttctgta atcagccaaa aagaattctg cttcgttagt
    41821 aaaatacgat gtagtactta aggcaacaat tttcaactta taccaatgtt tttcggtttt
    41881 ataaaaatca ttatctgcca tgtataaaga agcataagta ttcaaattta catacattcc
    41941 tggaattatg gcttcaggat tttctttact aagttttatt actgaaggga ttggagcaaa
    42001 ggtacttacc caatcaaaac ccataaaacc gaccacataa ttttccgaag cattaactcc
    42061 tgagccagaa gcagacgaat actgatttgt agaaccagga gtagtgacat ctgtttcatt
    42121 agtataagca aaaccatacc acatccctcc ataatcaggg ctgtaagaat tataaaactt
    42181 caaataatct acttcgaagc ctcctgcatt atcctcgccg gcataatgcg attcgggagc
    42241 taatgtaaga ctttcaaagt caacaagatt tacaaatgtt tgtgcaaaaa caaacgaaac
    42301 actaagcatt aaagctaaga ctaaaggtaa tttttttctc atcattaaaa aatttaaaaa
    42361 ttaaacaaaa aaataaattc actttgtcaa agtctcaaat ttgcgggaaa ttttgtaatg
    42421 acattacttc attaagcttt ttgtcccgaa agcttttaac tatgactgtt atggcaggtc
    42481 ttctgactta ctctgagttg tgttgccttc ccacttcgtt tggcgaagca gtgactttgt
    42541 tacacaacat ttttaagagc ttacagcagc gggtactgta caggattttc acctgtttcc
    42601 ctcttagttt cggaaaattc cgaaaaacca ataacgccac aaagataaat gaattaatat
    42661 tataaaaaaa taaaaactat atattttttt aaaactgata actaaatttg aatacagggt
    42721 ttggatatgg actaatagag gtttcgttaa aattgtataa aatcatcatt aataaataac
    42781 tccgcccacc aataggagtt tgaaatgcaa aacccaacat tggcgaggct acataattta
    42841 tttctctacc tggaatagaa taataatttt ttatgttcac cacattatat tctgcataaa
    42901 tcaaaattcc atttccttca agaaatgaaa ttgcatttcc caagttttta ataatagtaa
    42961 aacttgcaaa tatatttcct ccatacatac taccctcata tccataataa tttgattttg
    43021 caaaagtgta gtttcctcct attccactcg acaagcgatt tgtaattcta tatcccacga
    43081 taggatttac ggcaatatat gttgcataac caaaagaaaa gcctatatct cctccataaa
    43141 aaattctttg aacataagtt gggtcgttct ccaaatattg cgactttgct gattttacaa
    43201 aacctacaca caagaaaagc acaactaata ttttcaaaaa tttctgcata aaacttgggt
    43261 aaagatatta ttcaaatttt tctttacaag caattattaa tttaagttca ttgtaattaa
    43321 actcgtaatt tgatttttcg accaaatcgc gtaagttgag gttttcttgt tcttcaataa
    43381 agtttacaat tttatcaagt cgcacttgct caacaagttc cgttatttcc aattctcctt
    43441 tcagcacaaa atgagacaaa tgcccaaaaa ttgttgagaa aacaagactt ctttcttcag
    43501 caatttcttc tattgatttg ccttgcttaa atagctcaaa actaattgtt ttagtatctt
    43561 ttttaggctc ttttattaat ttttccgctt gtgttttttt tgctttagtt ttggaaataa
    43621 tttcctcaac tggctctgcc ctttttatat tatttctttc gcaatactca taaattatat
    43681 cgagaatatc atctccaaat tgttttagtt taactttgcc aatcccttta attttaccta
    43741 attcagttag attttgtggt aatttttcca ctaattctct taaagtttta tatggtaaaa
    43801 ttttataaac atcaattcct gaatcttcag ctaaagactt tcgccatgcg tttaattctg
    43861 caaacaaatt tttattttca gtatgcgaag aataaaccgt aggtctgtta aattcagatt
    43921 taaaatcaac ttcggtatcg gataaagctc gtaaatattt taaagtttca aatttttcaa
    43981 cagaagcctg cattgctcct aacttcaatt ttaattctaa ttcaaatcta ttaatggcat
    44041 tatccatttc attgcttatt tctttattgt cgcaatcaaa atataagctt ttgaacttgg
    44101 aaataaaaat aaaattaaat ttctcgatat aatacttaga agccttggca attctatcta
    44161 aaatttgctc tgaattttca ggtaattctt ctttatttgt attaaataaa tagtttaatt
    44221 gcttaataaa tttatcatta acgtcaaaaa tttcgctttg tgcttgtgct gtgatttcct
    44281 caaatatttt cacaaacccc tcaccaaatc ttaaaatatt ttcagtataa attttcttaa
    44341 ctgaaaaata tctctttttc agatttaaaa aatcaaattg ttctaaaatt aaattttgtt
    44401 gataaatcaa ttttgaatct tgtagttttt cttggtcggg tgcattttct gcaacatttt
    44461 cattgaatct cgaaattgtt gaatctgttt ttattgccga acttggaatt tttgaaatta
    44521 aaaccaaacc ttctaacgat ttacaacggc tcaaagcaac atacacttgt ccaaaagcaa
    44581 aagcagcatt tacatctata attgctttct caaatgtcaa accttgactt ttatgaacag
    44641 ttattgccca agccaatttt aaaggatatt gctcaaaact acctataact tcttcatcaa
    44701 tttccttagt ttcgttattc aatgtatatc ttacattttc ccacatttct gttttaacat
    44761 aaatttcatc ttggttatca cattttacaa caattaagtc ttcaacaaat tttgtaataa
    44821 ttccaatctt accattataa tatagtttct caaatgaaga atcgtttttc acaaacatta
    44881 cctgagcacc gactttaagt tctaattcat attctgttgg atacatatat tcaggaaagt
    44941 cgtcttttat ttttgcttta aatttttttg aacgcaattt tattttcttt aatttttcat
    45001 cgttaatctc ttgagcatta gcattatgcg ttgttaatgt tatatatcct tcattttcat
    45061 tcaaattaaa atctggctca tatcgagagt ttaaaagctc aatagacttt tgcgaaactt
    45121 tattttctcg aatttctcca agcaatgaaa taaaagtctc atcagactgg cgataaatat
    45181 gttttaattc tattgtaaca agttcggttt tttgcaatgc tttagaacta aaaaagaaag
    45241 ccgaatcata aaaatctcgc aaaatattcc attcatcttc ttttactact ggtgctaact
    45301 gatgcaaatc gccaatcata agcaactgta caccaccaaa tggtttatca gtatgacgat
    45361 attttcttaa aacatcatct acagcatcta acaaatctgc acgcaccatg ctaatttcgt
    45421 caattaccaa caaatcaaga ctttgtataa gtttaatttt attcctactt aatttatagg
    45481 tataagcaga attgtcagtt tgctcatttt tagaattttc agatattgta gtataagcac
    45541 taataaaagg cgaaaatggt aattgaaaaa aggaatgtat tgttactcca cctgcattaa
    45601 ttgctgccac acctgtaggt gcaaccacaa ccatacgctt aggacaatat aattttaaat
    45661 tttttagaaa agtagtcttt cctgtgcctg cttttcctgt taaaaatata tttttatgtg
    45721 tataaagcac aaattcttgt gctaatttta attccttatt ctcaaaattt tccatatggt
    45781 gcaaaattgc aacaataata caaaaaaaaa ttaaaaaaaa ggaatataaa tcagttaaaa
    45841 gttaaaagag tttttagttt ttagttttta gtgtttagtt gttagtgttt agttgttagt
    45901 gtttagttgt tagtgtttag ttgttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    46021 nnnnngtgtt tagttgttag tgtttagttg ttagtgttta gttgttagtg tttagttgtt
    46081 agtgtttagt tgtttagtta gacaacttta aaaactttat aaacttttaa ctaattttat
    46141 tgtgttgccc ttgcgggcaa tcctttgaca gacttgtttt gtactcacgt acaacccaca
    46201 ttagctcact caaacgccca tgtcgctcgc tcggatttac aatccgagtg cttggttatg
    46261 ttacactcgg attacaaccg aagtccgtag gacgagctca gcgaagctaa atccgaacga
    46321 gcgagggact ttattctttt acgaattttt gaattttatt atcagttttt atgaaatata
    46381 ttcctgattt taaattgctg atatttacac ttttaactgc ttctgtagtt ttatacaata
    46441 ctcttccttg tatatcaatt atttcagctt gagtttcttc gttaaaatat aagttatctt
    46501 ttgctggatt tggataaatt gacaaagtat taagttgatt tttttctaaa gatgctgggt
    46561 cttcgcccga ttttgagttt gcatttaagc gtgtaattac aaaattatca ttataaaatc
    46621 caccaacaat aattttattt tttccttgca ttttcatatc gtgataaata atatcttcaa
    46681 aagtaaataa attattgtca gatattccat tttcgttaac acttataatt ctatcgtgat
    46741 tacctattat ataactttcc caatttatta aaactggatt aaattctgta tatccttgaa
    46801 tttcagaata ataaaatcca ttttcgccaa aatttgtatc aattacacca gttgtcgaaa
    46861 atttacttaa aaaagtttac cttctttacc tttaccagaa acaataatat ctccattatt
    46921 ttctataatt ttattaaaat attcataatc caaattaaaa tcttgcacaa tatccataag
    46981 ccatactcca ttatttgcaa aatctgttac taagtttccg ttttggtcta atttacataa
    47041 tgccaactca taattaccgt ctcctttaaa ttcattaaat cctgctacaa ggacagactg
    47101 gtcgtctaaa acataaatag atagtggaac aattctatat gttgcattat ctgttaaata
    47161 agcatttcct tcttgtccaa aactttcatc taaattccca tcgtaattat attttgagat
    47221 ataacattta acttctgaaa aatcaagatg atttcttttt ccgactaata gaaaatcact
    47281 gcttccaaaa cttgcacaat ttgcataaac atcatcattt aatgctgtat tgattattat
    47341 tttcccatta tttccaaaac tttcgtctaa atttccattt tcagcaaatt gcataaacat
    47401 tgcttgtgaa tcgccaaaat aggtttcgct aaaatagcca aatactaaaa ttttgttttc
    47461 attagttatt tgagaactat atattcttgc atttgcataa tctgtcaaga tcatcactcc
    47521 ttcgttccca aaagtttcca taacaactcc atttgcatct gtttttatta tggataaata
    47581 atgataatca ctaggttggc ttccaatttt gcttgctatt gcaattatat ttccgttatc
    47641 atcaaaatct ataaaataaa catctgatgc atctacgctt gttattactc cttgatttcc
    47701 aaaagtatta tcaagcgaaa aagattgtga actagcactt gtagctagca ataaaataaa
    47761 aatagttgaa catagacttg ctatggcttt ttgtagggta ttacctctct taaccgcaaa
    47821 aagatttgtt ttttagtttt ttcattttac aacaaattaa attaattatt aaacaacaat
    47881 acaaatatag catttatttt tataaaaagc aaatttttgt gtttttttat ttagggtaga
    47941 ttgacatttt ttgttagtat gctttttttc ggcattaaaa ttgcatttat aaaattaatt
    48001 ttttgtaatc aaatacaaat attattttta cggtctaaaa ttttgaaatt atgtttttag
    48061 aaggaatatt atcaatttcg ggtcaggcag ggctttttaa gttaatttca aaaggcaaca
    48121 acaacgttat tgttgaatcg ctaaatacag gtaagcgcat gcctactttc acaagttcaa
    48181 gaattagcac actttctgat gttgctattt acacagacga caatgaagac atctcattac
    48241 gagaagtttt aattaaaatg tttgaaaaat ttaatggtga aactataaaa ataggtaaag
    48301 attcaaataa agaacttgcc caaatgttag aatctgtact tcctaattat gacaaaagta
    48361 gagtttatat ttccgatatt aaaaaactta caaattggta taatagctta attgagcata
    48421 aaatactaac taaggaaaat ataaaaatcg aaagtgaaga agcaaaaaaa tctgctgaaa
    48481 aagaaaataa tgctactgaa taaaataaaa aagctgtctt agaaatttct ttgacagctt
    48541 ttttattatt tgttcttgaa aacttatttt agaatagttt tttattaatt ttattaataa
    48601 tttactgaat gaatttcaaa ataagcttct gggtgtttac atgctggaca agtttttaat
    48661 gcttttttgc ctttatgtac atatccgcag ttgcgacaca tccattctac ttcttcttct
    48721 ttttcaaaaa ctttgttatt ttttacattg tcaagaagtt ttagatagcg tttttcgtgt
    48781 tcggcttcac cttttgctat cattctaaaa gaagttgcaa tttctttaaa gccttctttt
    48841 tctgcaatat cagcaaatgc aggatataat tctgtccatt cttcattttc gcccattgca
    48901 gcagctttca agttttcggc agttgttcca actttaccag ctgggtacat tgctgtaatt
    48961 tcaagatttt cgccagactc taaatatttg aaaaaagttt ttgcatgttc aaattcttgt
    49021 tctgctgttt ctaaaaaaat cgctgcgatt tgttcaaaac cttctttttt tgcagttttc
    49081 gcaaacattg tataacgtga gcgagcttga gattcacctg caaatgcttt caataaattt
    49141 tgttctgttt ttgttccaat aatactcata ataaatttta attttaagtt atactttttt
    49201 taatattatt ttgtgttaaa aataaacttt ttatttgtaa ttttgtttac aaaataaaca
    49261 ataattttat aatttaaaaa cattctaaat attacgacta tgttaaattc aaaaatcgaa
    49321 aaaattttaa acgagcaaat cgaaaaagaa ggcttttcag ctaatcttta tttagctatg
    49381 gcaagctgga cagaaactct tggaatgagt ggtacagcta gttggttata tgcacaagca
    49441 gaagaagaaa aaatgcatat gatgaaattt gtatcttata ttaatgaaag aggtggtcat
    49501 gcaattattc ctgcattcga aaaacctgca agctcttgga aagatatttt tgttatgttc
    49561 gatgaagttt taaaacacga acaatatatc agcaagagta ttaatgacat tttagaatta
    49621 gcaataaaag aaaaagactt tgctactcaa aattggatac aatggtttgt ttcagaacaa
    49681 gttgaagaag aatcttctgt tcaaggagtt attgacaaac ttaaattatt aggaaaagca
    49741 agtctatata tgttcgacag agatattatg tcgttcagaa atgacgtaga agacgctgaa
    49801 taattgaaaa gtataaaata atttatattt ttatgtgtaa gccacagaat taaagtgatt
    49861 gtaaatttat aaaaatgaaa tttgtacccg aaagatataa aataaaagat gaaagaatga
    49921 aatctttcat tgataccgaa gaaattgaag gctatttaga agaaaccaaa aatccttcta
    49981 aggaagaagt tctagctgtt attgaaaagt cattgaataa aaatagatta aatctaagag
    50041 aaacggcaat tttaataaat gctgaatctc cagaattgat taatcttata aaagaaggtg
    50101 cacgcacact aaaagaaaaa atttacggaa accgcattgt actttttgct ccattatata
    50161 ttggaaataa atgtaaaaac aactgtcttt attgcggttt caggatttcg aacaaagatg
    50221 caaacagaaa aactttaagc gaccaagaaa ttgttaaaga aattgaagct cttgaagaca
    50281 atggtcaaaa aagattaatt ttagtttacg gcgaacacga ttcttacaat cctgaatata
    50341 ttgcccatac tgtacgtttg gcgtatagtg ttaaaaaagg cagaggtgaa ataagacgtg
    50401 taaacatcaa tgcagcaccg cttgatattg aaggttttag aacagttcag cgttctggaa
    50461 ttggaactta tcaagttttt caagagactt accatcaaga aacttataaa aaatatcatg
    50521 ttgcgggtcc aaaaaaagat tatgattata gattaacatc tttggataga gcccaagaag
    50581 ctggcttaga cgatgttggg attggtgctt tatttggttt gtatgattgg cgttttgaag
    50641 ttttagcttt agttcgccat gttaatcatc ttgaagcctg ctataatgtt ggtccacaca
    50701 caatttcttt tccacgaata aaagatgcat caggattaaa tattgatgat aaatatttag
    50761 tttctgacga agaatttgta agacttattg ctattcttag acttgcagtt ccatatacag
    50821 gattgatttt aacagctcgc gaaccagcag ttataagaga cgaagttatg cgttttggtg
    50881 tttcacaaat tgatggtggt acaaaattgg agttaggttc ttatgcaaat tcacaaaacg
    50941 aaaaacaaga tttaaacaaa gaacaatttg aaataaacga cacaagatct ttgggcgaaa
    51001 ttattggcga acttttagac aaagattata ttccatcttt ctgtactgca tgttacagat
    51061 taggtcgcac aggcgaacat tttatggaat tttctgtacc aggatttatt aagcgattct
    51121 gtactccaaa tgctctttta actctaacag aatatttgat agactatgca agcgatgaag
    51181 ttaaagaaaa aggttggaaa caaatagata aagaaattgc taaaatagaa gacccaaaac
    51241 ttgcagaatc tattaaagaa aaaattgaaa aaattaaaca aggcgaacgc gatttatatt
    51301 attaaaatta tgaaaaaaga attaaatata gtagttatca agatagccga cagaataaaa
    51361 gaagctggac ttgttcaaaa aactctaagc gactataacg aaattatcaa aacaagattt
    51421 ggatttcatg agcttagtac agaagtttgc agtcggcaag ccttgataat tttagagcta
    51481 gcaggcgaat tagagcaaga aaagaaatta gtttccgaac taaatcaaat tggtggtatt
    51541 aaaacagaag taatgaattt tgaattataa ttatggaata caatattgta agtattttgg
    51601 taaaacagcc acaattagat gcaagtaaat tacaaaactt atttcgagat tacggctgta
    51661 ttattcgaag cagattagga ctaaatcaaa cagcagttaa aggtggaatt attatattag
    51721 aactttcagg agattcaaat caaattgatt tatttttgga agatttaaaa acttttaaag
    51781 gaatagaata taaatttgtg aaaatttaaa ggaattatat atatttgtga aaatttaaag
    51841 gaattatata tatttgtgaa aatttaaagt aaatgacgaa aattcttgct ctatctgaag
    51901 ctaatgcgat tgcttttcat ggaatggctt taattgcaaa atcaaaagtt gctataagtg
    51961 caacagaaat tgctgaacta acaaactcat caaaacatca cacttctaaa gtgatgcaaa
    52021 gattggtaaa atctggttta ataggttcaa cacgaggacc aagtggtggt ttttttctaa
    52081 agaaaaagcc aacagaaatt aaagctattg acatcttaga agctttagaa ggcaaagttg
    52141 accttaaaga ttgtcctatt gacaatattc actgtccttt tggctcatgt ctattaggta
    52201 atgtatgcaa cgaaattgga acaaaactaa ttaatcattt gcaaacacat actttacaag
    52261 atatgattaa taatatagct aacgaagata ttttagtaaa gaaataaaag caattaccaa
    52321 aaatatcaac actcagaaat ttgtctgagt ttcatgagca ggcacataca catctaaaat
    52381 actactacaa actcaattat ttttcctata aaacaccatt aaagcatttt ttatttatac
    52441 agaaaataaa atcttacaaa tcaaatgtta ataaaaaaac atatttta
//
LOCUS       DAOPNX010000006        49743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_901_length_49743_cov_7.180788, whole genome shotgun sequence.
ACCESSION   DAOPNX010000006 DAOPNX010000000
VERSION     DAOPNX010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 49743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49743
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_901_length_49743_cov_7.180788"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..655
                     /locus_tag="PKN53_01255"
     CDS             110..655
                     /locus_tag="PKN53_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87614.1"
                     /translation="MKPTLKILLAISIVSTILTMSACKKTYDEGPNFSLCSKMNRITG
                     KWRLVSMEGVERLNPEVEQYMILTKDEIYDGNYKARFINFQEETGYAYGSIGEGLLTA
                     DGYWRFFDGAFGSSCNIGEYLDKNEGLVLIIDTTIGSCARDSWKILKLTNKELKIVNN
                     VCEMRPCTFYWNDRTLTFEKE"
     gene            821..7201
                     /locus_tag="PKN53_01260"
     CDS             821..7201
                     /locus_tag="PKN53_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87615.1"
                     /translation="MLVSFSYAQLKPEVKLTVDEQNLYNKSYNYFFEEDYISANQGFS
                     QLVSLYPKEEVFSYYYGACLVKLAAEPKSGVKYLEYSANKGINTANFYIGLANHFMYD
                     FEKALSFYDIYQKNASQKELVNFELEQYINMANSGRQLVKYAYELKLVSLKRVSKKNF
                     HYSYNLNDFGGNIIVKTDQFRTKIDRKIMETDLMYISDEHNVLFFSSYGDSKRNSLDI
                     YMSRKVNGNWTAAQKLPETINTKYDEAYPFLSEDGKTLYFASKGHNSMGGYDIFKTNW
                     DNENNQWSEPKNLDFPINSPFDDIMYAVDKFGETAFFTSTRESKTNECGVFRILVETN
                     PKKREMRNIDDIYLSANPSVSELAISELSNRQKTRTELSDTIIIKDVQTIEVKNLESD
                     IDLAIQEMFAKLDEISGEVSEYKKYASTANKLSQESLSEIKKINSEVSKLKTANAKAN
                     EAKIQDLQNQINDLGDRAIQLNEIAKYFYDFSVRTEGLLNYYLAEVKVLDKTSRNEKS
                     LEKSVYLTQAEINKIDRSFPLDKYIDELNKIINSENDKLAKYDKLNKPLFSELEQLNT
                     KITTKIEVAKSETEFELREKYIYDIKTYENQKIDLIDKIKENQIQIEFAKFDIAENNK
                     KIAEANLLLEEIKGNLFLDPNLNLSKANNDIEVLKSHKSQTNLKELLNVQESINTDLS
                     LYSPIIDYDAILYGEDLANNEVVIDKEEYINKFSNYISNETKTFGESLITNDSLKNII
                     ANLELDFDKTESPEEKQNIINNINSTQIKINENKNVLRENSKNISQASNQKNISNLEV
                     LKAKDEESKFKSEIAEIQNLISISSEIEEDIADFQILSANDESLDLWLLKLIKNDIDQ
                     NAEIKISELENQMKGEVLQIQSIMAEMRQRLSSNQNRINQSKPILAEFEKAKSLFAVS
                     ETLNKTQEKNQLISQANGIIEENLKNSIDYLNTSLQDFYSDYLLYSANIKKYDLADNR
                     TQSYSLKAEYHKQIADSLQILAEENSSESISLLGEAYKNIEIANLNLNYIFEFLENGA
                     KYVPNKNFGNRLETLNFINEITTIKTKELLPEEIEVIVELEPKNIEELILKSEQTSNE
                     IQALTAKFASSNDEEKDEILKDIEKLNQDIKLQLKTINEEIMQVQQRTINEQLAEIVS
                     NNPKAEISDLKEKIKHYTDDCNTYYETYDYYLLSEVVIRGANVLNQQNELLKNKKVII
                     LPQSRIDNLIANSAKFENFVDNESQILAENKKLIAEQSETKEQENIIQSEVIENKVDT
                     TEASLAINEPQPEIAVTISETVEPQAEIVENQPVTSETKSEIIEPKPEIAETKTEQAG
                     ESSQTQEIKPSDTERDLVVVGENIEVEKQTYNLDEFAQYLSSNTGDSEFLSKNTEINN
                     TQNQIDKNQTKILDLNNKIEQTNSQKQKSKLISEKSILESEQTKILIEQSQNKLSFVE
                     EVNETYAKTNPELINNSEYQKLIENYKDNRNSVISYSNFYSNEELIEIYEKANETENL
                     ILSEIAKNLKSQGFVELAKLETREIASNELKPENEISYQNNYEDEKTLNSLQDRLEKL
                     EISIKNNENQIQEYNKLISESSKNSQIKKHKKSINKIEKQQVKLIKDYAETAKNYEIL
                     TYNTRTKYFDEHLKSEDEIINTVSDSLKTQSEQDFEELISIYYKLLSFDSKVENQTVF
                     EEFEKAKKLSQLSFKKIELANEVLSFQDKNTEVLMPYYKQLVIEDLAENTETKTSVET
                     YVPEKEVIADSQENVEEEVIQVREKLDEIAQELETYDFYYRIQFAAYNEMVGEENFPT
                     LLPLFTERIENSRLIRFMTGTYFQLATAQQNLPRVRATGFEDAFIVAYFNGKRITIAE
                     ARRLEQELQVQKQEELLVSKDETTTETTNAEIETDETFVTPDKTAEPEPVVAETRTTI
                     NTELVQTTDVFYCIQVGVYRERINPERLYNLAPLFYDEYRPGLVRHVFGKYYDLNTAL
                     QEQNRIRQLGITDAFVTAYANGKQIYLYEARNLLANENLENKQEITLSEEHVAQAQVQ
                     AQERPEMQREPREPEQQQEVAKIEYYIQLGAFRNEPNNLVRDMFLKLAGNKKLYKTNS
                     NNLLIYRVGSFDKYSEVLTQLNNIKNLGVTDAFVLAFNNGKRINLTTARQLE"
     gene            7258..7962
                     /gene="tsaB"
                     /locus_tag="PKN53_01265"
     CDS             7258..7962
                     /gene="tsaB"
                     /locus_tag="PKN53_01265"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOD87616.1"
                     /translation="MNFILNIETSTDVCSVSLSQNGETKFLKIHNPNLENGEKASHSK
                     LLASYIQDILKENSILPESIKAVAISGGPGSYTGLRIGLSTAKGFATALDLPLIAIDS
                     LQIVAAMAKSKIDFSYDYIIPMTDARRMEVYTAIFDSYLNKISETEAKIIDENSFSEF
                     SNKKLVFCGNGAEKCKTVLMSENFIFLDDIFPSAEFMSDFSYKKFIGKEFVDLVYYEP
                     FYLKDFVATTPKNKII"
     gene            7974..8708
                     /locus_tag="PKN53_01270"
     CDS             7974..8708
                     /locus_tag="PKN53_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HOD87617.1"
                     /translation="MKFTSTSNPVLSEKAFEALSGEGTMSIKGTLNKSIILFLLLLVS
                     ASFSWKLATYDLELLSPLMWGGAIVGFILAMVTIFAKKYAKFTAPLYALAQGFVLGSI
                     SMVYSSLYSGIVLQAVLITFGIMAVMLVLFRSGIIKVTNKFRSGIIIATGGIALVYLV
                     SFILGFFGIQIPYLHSGGPIGIGISIFIIIIAALNLILDFNFIQESCERGAPKEAEWF
                     AAFGLMVTLVWLYLEVLRLLSYLNKR"
     gene            8708..9874
                     /locus_tag="PKN53_01275"
     CDS             8708..9874
                     /locus_tag="PKN53_01275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD87618.1"
                     /translation="MFEIAIVTEKNEKDFFQAALNIYENDENWVAPLNGDILGFFYSG
                     NKPREKKCNFQLWVLYKNKIPVGRISAFLPVKKHKANDIKAGGIGFFDCINSQEAANM
                     LFDVAVDWLKAENMQAVDANTVPGENFNHWGVLVDGFIPQAYGMPYNKEYYKELFENY
                     GFQNYFEQYSYHVDLQKPFPQRHIDFAQRVIDSGDFDFKHLDFKQTDKFVDDVTTVFN
                     EVWSYFHADHTKVERKEFEKMFKAMKPIVNPKFVWFVYKDGRPIGMEVCLPDLNQVIK
                     PFKGKLNLFNSLKLVSKIKKVKRARVIVFGVHPDYQRIGVTHALYVKMLGGLREAGVT
                     ELEMSWVGDYNQAVNRIYRYLGNSHLAKTHITYRYMIDENCKFERFTNEKQENQ"
     gene            complement(10142..11005)
                     /locus_tag="PKN53_01280"
     CDS             complement(10142..11005)
                     /locus_tag="PKN53_01280"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOD87619.1"
                     /translation="MQINFTNIFPSYELIDCGNGKKLERFGNIVLIRPEITASNKPHL
                     TYKDWKDMANAEFIETSKNSGYWNIFKDFEKPWYIEYKDEQIYIKACLNLTQSKHIGI
                     FPEQVLNWNFIKSISKNYQNMKFLNLFGYTGLTSIAAAPFSETITHIDSIKKVVDWAK
                     LNAKNSGFDNIRAICEDAPKFVEREIKRNNKYNAVILDPPPIGVGANNEKWILNKMLD
                     KLLKNISILLEEKSFVIMNSYSHSLSENAINTMIEENFPNHKTTLNEKLFGLSKYGNT
                     IDHGIFIHLES"
     gene            complement(11094..12848)
                     /locus_tag="PKN53_01285"
     CDS             complement(11094..12848)
                     /locus_tag="PKN53_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HOD87620.1"
                     /translation="MAKEIKFSLLYRDMWQSSGKYVPRVDQLKKIAPVIIEMGCFDRI
                     ETNGGAFEQVNLLYGENPNQAVREWTAPFNKAGIQTHMLERALNGLRMFPVPADVRRL
                     MYKVKKAQGVDICRSFCGLNDHRNLELSVKYAKEAGMISQAALSITHSKIHTVEYYME
                     IVEKLVEYGAEEICLKDMAGIGRPVTLGRLVSEIKASFPNVIVQYHGHSGPGFSVASM
                     LEVAKAGADYIDVAMEPLSWGTSHPDVITIQEMLKDAGFKVPNINMNAYMKARSLTQE
                     FIDEWLGLWINPKNREMTALLIGSGLPGGMMGSMMADLKDVHTGINAQLKAQGKKELT
                     TDEVLVMLFDEVQYVWPKLGYPPLVTPFSQYVKNISLMNVILMLQGKNRFEMIDKNAW
                     DMIQGKAGILPGPLAPEIIEIAKAQGKEFYTGVPQEAYPDELDKYRAEMIENGWDLGQ
                     DDEELFEFAMHERQYRDYKSGLAKERFEEELNSLKYKKAENKSITPDATKVEKNLVEI
                     KSPDKGKVYYDLSVELDREIIKPNDTITKGKRLFYLESNTHYQEILADFNGKLASVLI
                     KQGDLVDKDDVIALVEIN"
     gene            complement(12963..15332)
                     /locus_tag="PKN53_01290"
     CDS             complement(12963..15332)
                     /locus_tag="PKN53_01290"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOD87621.1"
                     /translation="MKKTALLSLFLIFSVSFLFSQTSPIITQSGIPVTTLIQDTLING
                     CVQASNVTANNGAYGYFRNPGNPHFPFKSGIVMSTGAISAAAGPNTSTSSSGASPGSG
                     SDPDLAALVSGANINDACVIEFDFIPASDSIVFNYIFGSEEFPEYANSSFNDVFGFFL
                     SGPGINGPYTNNAINIALLPNNMPVTINNVHNYNYYYAQPNAGSTSPGSYYGAVQFDG
                     NTILLTARAEVQACQTYHIKLAIGDRSDSSYDSGVFLEAGSFISGGAVSVANTAQHGD
                     ENDLWEGCTNYYVISRAEGVAVDQDLTIEMAIDPSSTATEGVDFTPFPESITIPAGEM
                     TDTIYYSAFNDGIEEGTESIIIAFFTSCPCGNQSMAIYDTIWIYDAEFIKGGIQDIET
                     AFYCGEEAPTTITLVGECNRDPFIDYHWSTGENTNSITIYPQLGSTTYYLTMLDECDN
                     EVLDSITILVNGLTLSNANIINPSCHNACDGIIELVVDGEDGPFTYKYAREPYHFFPD
                     SIHTTTNPVLSGLCPGNYRIEVKDMIGCFKRYQIELPNPPPVDLALGIAEDDVKSCET
                     LESVTFTAVSNQPNPMFTWSTGATTPSITVTPQVGETTYWVKIFDGCGEYKYDEVKVQ
                     YSQIEISSQTLDDTGECNGIATAFANGGVYPYTFYWESPIGNFGETQIGLCEGWYQVK
                     VTDAIGCSKIGVVNIALDTGNYVPQTFYDDIFTVFPNPTTNEFTIDYKKDDFNDISIN
                     ITDIKGSLILESKMTSNKSVIKNLKAGIYFVNLRDKNGIIAVQRVVVTK"
     gene            15456..15881
                     /gene="ruvX"
                     /locus_tag="PKN53_01295"
     CDS             15456..15881
                     /gene="ruvX"
                     /locus_tag="PKN53_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOD87622.1"
                     /translation="MKRYLALDIGRKRTGIAVTDTARIISSALETEETHKLEEFLKKY
                     FAENEVEKIIVGMPKQLNNQPSEAVKYIVPVLNRLKKVFKEYEFIEVDERFTSKMAFQ
                     TMIDAGSKKQQRQEKSIIDKISAAIILQSYLDSENYKKK"
     gene            15878..16429
                     /gene="def"
                     /locus_tag="PKN53_01300"
     CDS             15878..16429
                     /gene="def"
                     /locus_tag="PKN53_01300"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOD87623.1"
                     /translation="MILPIYLYGHPVLRKKSVDISKDYPDLKQLIENMFETMYKAEGI
                     GLAAPQIGENINLVVVDARVLAKDRPELQDFIRVFINPKITCNSEQKVTVEEGCLSIP
                     SIYENVNRCESLNVEYFNENFEKVTETLDGHKAIVVQHEFDHLQGIVFTDRISPIRRK
                     MLQKRLNNIAKGKVGVSYKVKIA"
     gene            16445..16969
                     /locus_tag="PKN53_01305"
     CDS             16445..16969
                     /locus_tag="PKN53_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87624.1"
                     /translation="MKIKCFFILIIIVGTLFSCGSKTKSDTKNEDLKNQIVEKEKETY
                     SSDSLNLDLANDLITMYLAYANEFPQDSLAPEYLFKAAEISMNINKAKNSIDYLTEIE
                     NNYKNYDKYVATLFLKAFILENYLKNFEDAEKYYSIVVEQYPEHSLAQDAEAAISFLG
                     IDDEELIKMFQQSM"
     gene            complement(16972..17199)
                     /locus_tag="PKN53_01310"
     CDS             complement(16972..17199)
                     /locus_tag="PKN53_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87625.1"
                     /translation="MKIKFIRTPFIQILENFRSINANTTKAIAEATKIDKLITEEKVK
                     FILITIPMIKGITILINGFLRRLVINMSYIA"
     gene            17138..19117
                     /locus_tag="PKN53_01315"
     CDS             17138..19117
                     /locus_tag="PKN53_01315"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOD87626.1"
                     /translation="MIERKFSRIWINGVLINLIFISSGMFLTQSLNEKAVESLSSNKQ
                     GNLIGVINKEPKIMEASTKVELNIIALKSQDQWLETSGSTILYIENNEDVNSLKVGDK
                     LIFSPDLQEIGNKGNPEEFNYKKYLSYNLILSTDYLSSSEWKILKENKNIKLQHRFSR
                     FRTKLIDLLKANNLHDDELAVVSALALGYQDNISYELRHAYSSSGAMHILAVSGMHVG
                     IVYGVITFLLSFLKSKKLRIPKIFLSIILVWAYAFLTGLSPSVSRAALMFSVAALGNI
                     QKHKSSSLNAIAASAFILLLINPYNLLNLGFQLSYIAVIGIILLYKPIYEIVKPKNKI
                     INSIWSMTAVSLAAQIVTAPLGMYYFHQFSNYFLITNYLLIPISTIAIWLIITLFAVS
                     FIPFLSAFIAKILVYVIKAMNYCALGIESLPFSVTNDIYINLPQLILLYLIIIFVFLF
                     FFQTKLYRHLVCAISFIIIFLTIGLFKDIESSKQKYFIVYNMNKNTVINIVNAKKNIV
                     FASLDDELEQNIEYTAKNNWLKKGLEHQKYVDISSKSNLFLTNLLSISDSEIFYKNNF
                     IYFSGLRIYVLNDNFKMLKSDEEFKKLDTDYIVLSNNPEIKLSEIAEFFNFDEIIIDA
                     SNYKNKTEKWIAENKDLNYKLFDIREQGAFVLKIE"
     gene            19182..21959
                     /gene="uvrA"
                     /locus_tag="PKN53_01320"
     CDS             19182..21959
                     /gene="uvrA"
                     /locus_tag="PKN53_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOD87627.1"
                     /translation="MQNTNKISLRGVRVNNLKNIDVEIPHDKLVVITGLSGSGKSSLA
                     FDTIFAEGQRRYVESLSSYARQFLGRMNKPEVDFIDGIPPAIAIQQKVNTRNPRSTVG
                     TSTEIYEYLKLLFARIGKTISPISGKEVKKHNVADVVEFIVNQEDGTRVLISFTYKFN
                     SEMSKTEQLALLEKQGYARVYCNDKLVKTEDVLNENCKGISKEIDIIIDRLVIRKNDD
                     LYARIADSVQTAFFEGNYECKVIILGEDKNKVSKFSNHFEADGIKFEIPHEHLFSFNN
                     PLGACPLCEGFGKILGIDEDLVIPNKTLSVYEGAVACWKGDVMQQWEKEFILRAGKFN
                     FPIHRPYYQLSEKEKDFLWNGNDAVYGINDFFSFLESQKHKIQYRVMISRFRGKTNCT
                     ECKGKRLKKEAFYVKINNHSIGDLIDMSFEELKVFFKNLKLSKVEKETASRLLEEITL
                     RIDSVLDVGLPYLTLNRLSSTLSGGESQRINLATIFGSGLVGSLYVLDEPSIGLHSRD
                     TQRLIKVLKNLRDRGNTVVVVEHDEEIIRAADYIIDIGPYAGENGGEVVFAGDVKKML
                     QSSNTLTAKYLKGELKNPIREKPSQWRNYIEIIGARHNNLKNINVKFPLGVITVVTGV
                     SGSGKSSLVKGILYPALKKQLGHFADRTGEHESLAGDYKLCSDIEYVDQNPIGKSSRS
                     NPATYIKAYDEIRKLFAEQRHAKANGYKASHFSFNVAGGRCETCLGEGEITVGMQFMA
                     DVHLVCDACNGKKFKEDILEVKYKGKNIYDILEMPISEAVEFFGSENKATEKKIASLL
                     QNYLDVGLGYVKLGQSSNTLSGGESQRIKLASYLSMEKANPTIFVFDEPTTGLHFYDI
                     GKLLLAFEKMRAKGHTLIIVEHNPEVIRFADWIIDLGPEGGDKGGEIVFEGTPEDLKL
                     SGTYTGKSLAK"
     gene            complement(21961..26973)
                     /locus_tag="PKN53_01325"
     CDS             complement(21961..26973)
                     /locus_tag="PKN53_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOD87628.1"
                     /translation="MIKRIFYRIFLTCFFVAVFCSAFSQLNYVENKGQWNEKVLYKLR
                     LNEAALFLEENCVTWALTDPHSMAHSHAHHGCEEHNNNLLKDAHAYKVHFVGANKNPQ
                     IQAQNPQLDYVNYFIGNNPKNWASNVKLYEKVLYTDIYNQIDLLYYTHEKGVKYDFIV
                     KPSGNYKDIILEYEGVDNIKIKDNQLVVTTSVNEVIELQPFAYQIFDGEKIPIRCNYK
                     LKKNKLSFSFPNGFDNSRDLIIDPSLVFSTYTGSTGDNWGFTATWDYNDNVYSGGIVF
                     DIGYPTSTGAYQINFAGGTAPIAGSSFYANGCDVGIIKYNANGTQRLYATYLGGSNGQ
                     EMPHSLIVTEENELVIMGTTGADDFPVSTNAFQTTFAGGDSVVYDNVIGFNNGVDIFV
                     TKLSEDGTQLLASTYVGGSGNDGLNFKPHMAIHYVLMHGNDSLYYNYADGARGEVIVD
                     GKNNVYVGTNTFSTDFPSGINPGYQTTSGGKQDGIVFKLNPDLSQLIWSSYLGGNEDD
                     AIFSMDLSPNEDVLVTGATVSHNFPVTSGAYNTSHNGGSTDAFVSKLNNMGNTLLASS
                     YFGSSAFDNAYFVRSDKFGHIFITGLTKAPANALVYNATYNHPNSGQFISKFNSNLSS
                     LVWSTRFGTDNGRPNISITAFAVDICNRIYLTGCGREWVSSPYNAQWDTFAWEDDYGT
                     KGMEITADAIQTETDGQDFYVMVLSEDASELEYASFFGELHYDGCSYSGHDHVDGGTS
                     RFDKKGHIIQSVCSSCGACQQFPTAPNPGVWSSTNNSSNCNNAVFKIRIIENLADASF
                     DPIPAGCAPYTVNFQNTSQGTSFLWDFGDGSPTSTALNPNHTYTVGGEYTVTLIVGDP
                     LSCNFYDTIQRTFSVIEPGLSTLPDLEICPGQSIVIGPEGNYPAGTTFSWVQGSNLNN
                     TTIKNPVATPTETTEYILIATGICVDSLIQTVSIYEPDIDLYASADTTICPGGTAHLH
                     ATSTGDVDSWAWSTSTSFNSILSNSQNLTVSPSQNTTYYVRVKEDVCNTFLIESVNVN
                     VHQFNYSIVPDQILCPGDETHLTITNNKPSDILTYSWQPSSTIISGANTNSPLVKPTT
                     PTTYTVTITNQMGCTTTNSVYVDIDNISWSPPDLTHNPCFGNCLGTATVSANGIAPYS
                     YAWSGNPSQNETASNLCAGTYTVTVTDGNNCTSETSVTINQPPQLLASFTSVQHPVCD
                     GIGYGSATVYPTGGTTPYTYSWSNGGGSNATNNTCFTGQNYVTITDNNGCQRVDSVFM
                     SSPSDLTSSLSNVVHISCYGFCDGEIHASVSLGTPPYSYLWSNDSTSTSITELCQGVY
                     TLTVIDANNCVTHQYQYIYEPPELLVTTQISEEIKCFGETGSVSASAIGGTYPHSYNW
                     SQGSTQTSLDNVSAGIYQVTVLDYNNCKTVSEIELTQPPLLEMNNIVTNMLCTGVCNG
                     SIHTELIGGTPPYYYSWSNGSQKANIENLCEDEYELTIKDKNNCSLTRNFTIINQDYI
                     PDLSVAASDTIIYRGQSVNLLALSSSSGYYLWDYSKFLSNIEISNPIATPDDTTMFYV
                     KFTDLHGCRIRDSIKIYVKEVICGDPYIYVPNAFTPNNDGKNDYFKPYYPYGLVTEVF
                     FVVYDRWGNIVFQTDEIWTKGWDGTYRGEQLTSDVYVYFLRARCLNGEEYSHKGNVTL
                     LR"
     gene            complement(27055..27870)
                     /locus_tag="PKN53_01330"
     CDS             complement(27055..27870)
                     /locus_tag="PKN53_01330"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HOD87629.1"
                     /translation="MQIQENKNLLIELAIKAGKETLKYFRKNFDVNLKADNSPVTIAD
                     ISANKIIIEGLKKTNIAIISEEIENLSYQDRKDLKKFWIIDPLDGTKQFVKGEDEYTI
                     NIALVCENKVVEGVVFAPAKSELYYGNVNSNSVKINTLTNKIENITLDHKIEIPKFVI
                     SKSHLNSNTTSFVNTFKEKFPESECRNVGSSLKLCAVASNSANIYLRIGQINEWDIAA
                     GHAVLKAAGGNIFSFQTGKEIIYNSESLKTPDFFAIKDFNFKNEIINTYKKLS"
     gene            complement(27874..28635)
                     /locus_tag="PKN53_01335"
     CDS             complement(27874..28635)
                     /locus_tag="PKN53_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOD87630.1"
                     /translation="MKKTALISGATSGIGKATALLLSENGYNLILTGRRKQRLDDLKS
                     EIEAKSNAKVFCLNFDIRSNDEVCKAIDSLPEDFKKIDLLINNAGLAMGLSTIQTGDL
                     QDWETMIDTNIKGLLYLTLKVAPLMIEQKSGHIINIGSIAGKEVYPNGNVYCATKHAV
                     DALSKAMRIDMLPYKIKVTQIAPGMVDTEFSTVRFHGDKERADKVYDGLTPLYAEDIA
                     EIILFVANRPKHVNINDILVTPLAQASSANVVRNS"
     gene            complement(28653..29570)
                     /locus_tag="PKN53_01340"
     CDS             complement(28653..29570)
                     /locus_tag="PKN53_01340"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOD87631.1"
                     /translation="MITSYQFVKFREDSFVEFHRSLTEINTVSCFDFEDSVLDFKTND
                     SNRYKSDHREKVLKFLNKFKVKILKTWLGFRINEYGTNRYFDDIEAIRSLGNIKIRCI
                     FLSNCSSVEVLKTFLESTEDIRYDEVIPIIESNEAFNNLEEIIKFKDPRFKNIAFGHC
                     DYNLSCDLFPFIHQDNPKYWYWIEKFATLCKKENIGFINSIYLNVNHNDYFTLMLKNT
                     SKFFDNWGQITLTTEQSKTCYNFKNDQTINLPEFNYMRTDSVEEFAGKIITLFEKYNP
                     EGKGYSVMPDTRTFISPHEYVAAKRFLNK"
     gene            complement(29763..31013)
                     /locus_tag="PKN53_01345"
     CDS             complement(29763..31013)
                     /locus_tag="PKN53_01345"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HOD87632.1"
                     /translation="MLQITNKKTLGINGLGRIGKLTLWYHLSKRYFDGIVINVGREVG
                     KKFEDLIQVISSDTTYGRLDNFLYGHSGKKVEIKIVDRDAGLISIDGFSVRFLYTDRN
                     PKDIKWREAGVKVVVDCTGKFLDPTVPANDPKGSIAGHIEAGALKVIASAPFKIKNIA
                     LDNPDEYPTLIYGINHNSFDAEKHHIISAASCTTTGLAHMVKPLLEDEETKRVLTASM
                     TTVHAATNTQSVLDSVPATGTSDLRKNRSVLNNIILSSTGAAKTLERVIPQATSFGFM
                     ADSIRIPTPTVSLISLNITFTSRLNELSMPFINAQYIKDIYKTAAAGAQKNLLYFSDL
                     QNVSTDLMGLNAAVIIEGNELHTRTGFLQLHPETLLQAGIKEANAVNLPVTHAKILGW
                     YDNEYGSYVYCLGQLAEFVDSQIN"
     gene            complement(31031..35131)
                     /locus_tag="PKN53_01350"
     CDS             complement(31031..35131)
                     /locus_tag="PKN53_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOD87633.1"
                     /translation="MKQEINSKALEVNLSETQKIKVEIPEKQLWFLSLSKNYYGINQR
                     TSDFLKELNHPYFNSKIIVDGLVKILNDEFWVYKELDEKEKVYDVLFDIFRDLLEKNI
                     SDKYTKSIVQNYLQFFSNNFDDIKSLKNGIEHFIKILDEEMESNLFAYLSNISFFRTC
                     LKKAVDNESSKELVFNFTKQLLLNNVEFWESTTKIEEWYESVKNKFSKDYSSTIYEIG
                     KKFYNYYKEKLNQANDYNELIKYSFTFSEIIESLRNRIVDFEEATDQFVYIFFLLHLP
                     GTIFHRDYILVDLNKAIKRISSECSTTQCINSIDILYSLFADFKYSNTSFILDSILTL
                     GKEIINTKNKELIHYFEEKTILFGFINPGVAYLTNDWELKVNPNHIKNIRVWQELIAY
                     DPFTMKKLLSSLIINLRIGGIFIFDTDLFQKDISKFLNSNISPIYKQVKQLLRIYPVY
                     FNEIGAEGQLRDVTTVVDELTNRNDKLIHFLRKQIHTEGSNSHIDITLEIIKFWYTKD
                     KSLLESIVPENVFVTIDPDGQWVDGVHKVLVKLCKLNNSSLEELINLDKNSLQAHLNS
                     FPDPDCKDCQRVALMIELYHLLREKYLFSTNNLGALLSKYHYIEQDTINSLLEYIEAG
                     NHFEALKTIYSIMKILNDRILDEKQSEAWENIYYKRHIAFGIPSMYGQYHEEKFEAMG
                     LTFRLEQIASSLVDRILDEIKTDYFTARTLKDVFLVLQLIREGLTLDGIYDQGLDSNL
                     KMLQYSLSSESFTIRQYINIFQFMEKNLKEIINNYFIRPYENQLPVIIPSYLDKEDRK
                     NKHTVRKLIIQHSEVFYRDLLSSAFLVQSLDAFIGRVLNKLRKMADTLSDVEVQNIMS
                     YNPDSVISPIYRETLEIDNQVFLGSKAYYLKKLYLNDFPVPPGFVITTEVFRRLNSIM
                     KIQSLYDEIENLIRYHISELERLTGLEYGNPEKPLLMSVRSGAAISMPGAMNTFLNVG
                     MNDEITEALSKKDNYPWTSWDCYRRLLQTWGMAYGLDRNDFDNIMLEYKTKYDIQRKI
                     DFNPKIMREIAFAYKKHLEDNNITFESDPFKQIMQAVLSVFNSWDSARAKVYREHMQI
                     ADEWGTAVIIQQMIFGNLHKESGSGVLFTHDTQDNIPGINLTGDFSFLSQGEDIVAGL
                     INTLPISENQRKRYYHNSPISLESGFPKIYNKLKEISYDLIENHGFAHQEIEFTFEHT
                     EPEDLYILQTRSMIINKQDKVEVFATLEKNMELVGTGIGIGNRVLNGRVVFDLKDLKL
                     LKEKYPEDNAMLVRPDTVPDDIELIFECEGLLTGKGGATSHAAVTAGGLGKVCVVNCA
                     DMLVYENEKKAVIRDVEFRSLDLIAIDGIKGNVYKGNYPLKTQNLF"
     gene            complement(35128..36141)
                     /gene="gap"
                     /locus_tag="PKN53_01355"
     CDS             complement(35128..36141)
                     /gene="gap"
                     /locus_tag="PKN53_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOD87634.1"
                     /translation="MKKEVIRLGVNGFGRIGKIVCRMLLSDNSFDLVQINDPMSAKMI
                     AHLLKYDSSHGQFDAEIGHGDDFITVNGKKIVVTNFTSPSQIPWKDNKVNIVVDSSGK
                     FKTATSLQGHLQMGAEKVILSSPGEDSSIKRTVVMGINQQNISNDDKIISNLSCTTNC
                     VAMVFEVLFRHFGVKSAFMNTVHPFTNNQNLQDGFHKDMRRARSAMNNIIPTTSSAIK
                     ATTLLYPELANRFDGFATRVPVADCSFVELTAQLNKKVDVEAINSAFLKESQGDLKKY
                     LEYTEDPIVSSDIQGNKHSAIFDSQLTRVIGDNFIQIVAWYDNETGYSARIVDLVKYI
                     SNL"
     gene            36467..38068
                     /locus_tag="PKN53_01360"
     CDS             36467..38068
                     /locus_tag="PKN53_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOD87635.1"
                     /translation="MKFRIFGLVLSAILFFNFATYSQDVNSETRKFNKMLTLIDAMYV
                     DTVDMKKIVETAIVGMLKEMDPHSVYISADEIEKMKEPLEGSFEGIGVQFRLMEDTLL
                     VVGVISGGPSEKVGILTGDRIVVVDDEVIAGVGIDNAGVAKRLRGKKGTVVKVEVVRR
                     GEKQNLVFNITRDKIPIYSVDAAYVTKDKIGYVKLNKFAKSTTEELDSVFVTFAKAGV
                     KDIILDLSGNTGGYLDKAVNLCDEFLQSDKLIVYTEGAKIPRIEYKSTKKGRFYRGKL
                     VVIVDEASASASEIVSGALQDWDRAVVVGRRTFGKGLVQREMMLTDGSAVRLTIARYY
                     TPTGRLIQKPYDEGYDEYSADIVNRYKHGEFLHKDSIVFADSLKYKTLVNKKTVYGGG
                     GIMPDDFIPVDTTLRSDYHIELLRKGVLFSFEAKYVDTHRAELNEKYPDVETFIKKFE
                     VTDQILEELLAQAKEAKINVDSLPAATKEQDKYLRTHLKSLIAGDIWKNNEFWMIFNE
                     QNPFFLRAQEIILDNKLYDSLLKGE"
     gene            38068..38661
                     /gene="pnuC"
                     /locus_tag="PKN53_01365"
     CDS             38068..38661
                     /gene="pnuC"
                     /locus_tag="PKN53_01365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOD87636.1"
                     /translation="MTQIINWLITNWVETIAAFLGLLGIFLQIKQNAWYWFTAIIMVL
                     LYIYVFYVSGFYADMAFQFYYLVVGIYGWIHWVRTKNIAKNEKLQVFKLSKLHNIFAL
                     LASAVFFGIIYFILVNFTDSQVPLGDAFTTALSITATWLLARKILENWLYWIVVDLVS
                     TILYVYKSLYPSAILFSVLTILAVVGYFKWRKALVYE"
     gene            38654..39175
                     /locus_tag="PKN53_01370"
     CDS             38654..39175
                     /locus_tag="PKN53_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD87637.1"
                     /translation="MNKTLKIVLTGPESTGKTTLAEQLSSHYSLPWVEELARAYVESL
                     NRPYNQADVLEIAKLQITQEHKALAKNHKAVFFDTDLIITKIWLLHVYGTSPSWIDQE
                     ISNYKNCIHLLCYPDLEWVADGVRENPDIRLYLFEKYELEIKNYGFHYKIIKGSEDGR
                     LKRALEFVNLYLR"
     gene            complement(39317..40453)
                     /locus_tag="PKN53_01375"
     CDS             complement(39317..40453)
                     /locus_tag="PKN53_01375"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOD87638.1"
                     /translation="MNIPMVDLKSQYLKIKDEVDARIAKVIENTEFVNGTDVKEFKKE
                     LAEYLNVKHVIPCGNGTDALQIAMMSLGLVPGDEVISSDFTFIATVETIALLKLTPVM
                     VDVDPNTFCIDPDRIEKAITPKTKAIIPVHLFGQSCDMDRIMDIAKKHNLFVIEDTAQ
                     AIGSDFTSAKGWTKKVGTIGDIGCTSFFPSKNLGCYGDGGAIFTNNDELAEKITSIAN
                     HGMEVRYYHDRIGVNSRLDTIQAAVLRTKLPHLDNYNTARRKAADYYDKAFENCSKIK
                     TPTRSKFSTHTFHQYTLVLNNVDRKALMEHLSSKGIASAVYYPVALHNQKAFQDFKYN
                     SKDFPVTDKLCESVISLPMNTELDDKTLKYICDSVLEFVEKNHS"
     gene            complement(40510..41388)
                     /gene="dapA"
                     /locus_tag="PKN53_01380"
     CDS             complement(40510..41388)
                     /gene="dapA"
                     /locus_tag="PKN53_01380"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOD87639.1"
                     /translation="MKTNFIGTGVAMVTPFTEDKQIDFPAFAKLINDLIANGVDYLLV
                     MGTTAESATISEEEKQEILEFAKKEINSRVPIMYGIGGNNTMDVINRIKSTNLNGVDG
                     ILCVAPYYNKPNQEGLYQHFATIASVCPTQLILYNVPGRTGVNILPDTVVRLANDFEN
                     ITAIKEACGSIDQMMQVIKNKPRSFTVISGDDGLTLPLIAVGGEGVISVAGNAFPKEM
                     SQLVRLALNKDFENAKTIHYKLLDSISNMFAEGNPCGVKEYLSQQGKMKNEFRLPMVP
                     ASQKLSEKIKNDLRRY"
     gene            complement(41395..41946)
                     /locus_tag="PKN53_01385"
     CDS             complement(41395..41946)
                     /locus_tag="PKN53_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87640.1"
                     /translation="MKFIERLRYKIGLRKFRKTARRPEANAIACNLYKAKSIGIVYDA
                     TLNEDFEKVKTFVEELKSKIHKVDSIGYVDSAELSEFHTKHEDFKFYSKKDLNKFFKP
                     ISEITKEFCNCKFDILIDLNINEKLPNCFIVAESQAMFKTGLYSKNEPVYYDLMINID
                     KNTENLLEELIKQTEHYLKMINT"
     gene            42012..42344
                     /gene="rnpB"
                     /locus_tag="PKN53_01390"
     ncRNA           42012..42344
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKN53_01390"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            42359..42691
                     /locus_tag="PKN53_01395"
     CDS             42359..42691
                     /locus_tag="PKN53_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HOD87641.1"
                     /translation="MMTNEWKKREGIVYSTKADFVYQTNEIEEPETLPAEKQKLKIAL
                     DRKQRKGKTVVLITGFVGKSDDLENLAKELKNKCGTGGSAKNGEIIIQGEMIEKVKDI
                     LRNKGFKV"
     gene            42699..44117
                     /locus_tag="PKN53_01400"
     CDS             42699..44117
                     /locus_tag="PKN53_01400"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOD87642.1"
                     /translation="MKIKNLILVSTLILISAWSCGDKKSKTEKQDEHLIEIALNIANY
                     SNDYIVATCNEQVDTVKVGEDGKYLYSTFLGQDSYLEIKIGRKRVLIYTKPKAEMQIE
                     VDVQDFHNSLKFSGDYAEINNYLAKQSKLLFDAKPASENFIYAGDYSTFSNSFKKYDD
                     ESKNNLNKFVEANPNFEDFVILEKERQKLINTILYLSYYSPLVYTEQSDEALENEINS
                     LINSTDKNNPKLLCLRIFYEYAETLIPYKLNLKYREAGIENIDDAEYINDYFDLIGEL
                     FSESEVYEAVFYSGLKSFIMYYGTESVSEQFSKYEDFSMNREHLSELNKIFAEYDKLK
                     PGKPSIDWEFPDKDGKIYSSKDFAGKYIYIDVWASWCGPCVRESKFYKEIATKFKGKN
                     IAFVSISVDENKDEWTNFIGNENNNIVNLWAGGWNNDLCNFFKINSIPRFILIDKNGL
                     IINANASNPSGDIEEVLNNLDL"
     gene            complement(44129..45790)
                     /locus_tag="PKN53_01405"
     CDS             complement(44129..45790)
                     /locus_tag="PKN53_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87643.1"
                     /translation="MKFKIINILFLVIFATSYTFANITDSLNSKYINYRNRLLNEWVI
                     ISSDVEQFGVNIPAVDRRLDKAGNISWVSWSDGNSNFNHWLGLLSTEYRLLKNNNQDY
                     QETLSMLLYAMIALERLDLYSEYTLRKYHKTEPNKSDFELIKYPEDINGFLIRDDVSL
                     GFWKQYYKHFNEEYGKYNKIKNGTNRYLSIFQKGVIPQQGMSQDNIVRMLQGLALVKK
                     LVDDEDVSKIKVKFRNDFIPNYLKSKGIWQGNTISFKKWVDDLTDRLVRQCHQEYPQQ
                     RIVLKPKNEFKARPSQTRFGALFSSYWYIMNPVTNDLVAEGNGEDMGVWINSYGFGEA
                     GKSITGNDIYHYDNSHKGLAAYVFKSILFKNLKILWLAGFPMPENFDDYMFRDLASIS
                     DINWNTKSYDLLFLLSDKRNKYTYEHCPLILVTLHNQKYQNIYKPGNQLYENDKIFYQ
                     QLLSFAPFSGPSSDTSRNDYHPYWSASSRLVWPNNEGNKSSTRHYEYVGMDYMFLYNL
                     YRLVFEPNNYSLKDNTILKTKTISVYPSPELDDEEVEYFYLPPEK"
     gene            45907..46830
                     /locus_tag="PKN53_01410"
     CDS             45907..46830
                     /locus_tag="PKN53_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOD87644.1"
                     /translation="MRILFTIYCFFIAGLAYSQITPEQYVSTYSDLAVKEMKRTGVPA
                     SITLAQGMLESGNGNSYLATKGKNHFGIKCHKWTGDKIYRDDDAKDECFRKYKNVEQS
                     FIDHSNFLKENSRYHFLFDLEITDYKAWAKGLKKAGYATNPNYATSLINLIEKHELYN
                     FDLGISKPKEDKPTKRPERTKAPVKDDFIVNPYLNAYNYNNNVPYIVVVDTIEIADLA
                     RQLDMMRWQLVKYNDLDKNSILYPGDNIYIKPKRNKAEKQYNVHIVEQNETVRDISEK
                     YAVKSKKLLKFNDLTDIDQELKVGSEIKLRR"
     gene            47019..49580
                     /gene="gyrA"
                     /locus_tag="PKN53_01415"
     CDS             47019..49580
                     /gene="gyrA"
                     /locus_tag="PKN53_01415"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOD87645.1"
                     /translation="MLEEEKSSRVIRINIEDEMKSAYIDYSMSVIVSRALPDVRDGLK
                     PVQRRILYGMSELGLASNKPYKKSARIVGEVLGKYHPHGDSSVYLAMVRMAQDWALRY
                     TLVDGQGNFGSVDGDSPAAMRYTEARMAKIADEILMDIEKETVDFNLNFDDSLEEPSV
                     MPTRLPNLLVNGASGIAVGMATNMAPHNLSQTVDCIVAYIDNNEITSGELGEIMQAPD
                     FPTGGIIYGVEGVREAFETGRGRVVIRAKAEIEVGDNGREKIIVTEIPYMVNKSELII
                     KIADLINDKKIDGISNINDESDREGMRIVIDIKRDAVANIVLNKLYKLTALQSSFSIN
                     NIALVKGRPMTLNLYDLVKYFVEHRHDVVVRRTEFDLKKAEERAHILEGLIIASDNID
                     EVIRIIRASQSPDQAREALMERFKLTEIQARAIVEMRLRQLTGLEQDKLRAEYAELME
                     KIAYYKEILSNLSLRMQIIKDEMLEIKEKYGDERKTEIVYASEELNPEDFYADEDIVI
                     TISDMGYIKRTALTEFRAQNRGGVGAKGSTTREEDFIEHVFVASMHDVLLLFTKKGKC
                     YWIRAFEVPEGTKTSKGRAIQNLINIEPDDEIKAFMNVVAKRDDDYLDNSYIIMCTKN
                     GVFKKTRLREYSRPRQTGVNAINIREGDSLLTANLIEDKPGEEPVNIMIGTKNGKVVR
                     FPHDLVRPIGRTATGVKGITLDEGSEVIGMITVRTDQDVLVVSENGYGKRSSIDDYRV
                     TNRGGKGVKTINITKKTGDLIDISAVTDNDHLIITNKSGITIRLAVDTLRVMGRATQG
                     VRLINLRDDDSIASVAKIEDGALEEKNAENHEELDENNGENLENNDENANNDSKE"
BASE COUNT        17534 a         7276 c         8102 g        16831 t
ORIGIN      
        1 tagttgatga agaaagactg aaaatgattt gtgcttaggt cgaactcacg ttaattaggg
       61 aatatcaaaa aacagaaaaa ccgaatttaa tattttaaaa aatataatca tgaaacctac
      121 attaaaaatt ttacttgcaa ttagtatagt tagtacaatt ttaacaatga gtgcttgcaa
      181 aaaaacttat gacgaaggac caaatttttc attgtgtagc aaaatgaacc gcataacagg
      241 taaatggcgt ttagtttcta tggaaggagt agagagatta aatcctgaag ttgaacaata
      301 tatgatacta actaaagatg aaatatatga tggtaattat aaagcaagat ttattaattt
      361 tcaggaggaa acaggttatg catatggtag tattggagaa ggtttattaa cagcagatgg
      421 atattggcga ttttttgatg gagcatttgg aagtagctgt aatattggag aatatttgga
      481 taagaatgaa ggtttagttc tcattattga tacaactata ggtagttgtg caagagacag
      541 ttggaaaatt ttaaaattga caaataagga attaaaaata gttaataatg tttgtgaaat
      601 gagaccttgt acattttatt ggaatgatag aacattaaca tttgaaaagg aataatgtga
      661 gtaaaactaa aaaccacaat taatcctatt tttaatcttt ctcccatata ttataatcaa
      721 ttttacaaaa aaatattaat tttgctttat atttgaatta tgataaacac aaattggctt
      781 gaaaatttgt aaatacatat atttaaacat tattttttta atgttagttt cttttagcta
      841 tgctcagtta aagccagaag ttaaattaac agtagatgag caaaatcttt acaacaagtc
      901 gtataattat ttttttgaag aagactatat ttctgctaat cagggttttt ctcagcttgt
      961 gagtttatat ccaaaagagg aggtttttag ttattattat ggagcatgtt tggtgaaact
     1021 tgcagccgag ccaaaatctg gagttaagta tcttgaatat tctgctaata aaggaataaa
     1081 cactgcgaat ttttatattg gtttagcaaa tcattttatg tatgattttg aaaaagcttt
     1141 gagtttttat gatatttatc aaaaaaatgc aagtcaaaaa gaacttgtaa actttgagct
     1201 tgagcaatac ataaatatgg caaatagcgg aaggcaatta gttaaatatg cttatgaatt
     1261 aaaattagtt agtcttaaaa gagtttctaa aaagaatttt cattattcat ataacttaaa
     1321 tgattttggt ggaaatatta ttgtgaaaac cgaccagttt agaacaaaaa tcgatagaaa
     1381 aattatggaa acagatttga tgtatatctc tgatgaacat aatgttctgt ttttctcatc
     1441 ttatggagat tctaaacgta atagcttgga tatttatatg tctcgaaagg taaatggaaa
     1501 ttggacagca gcacaaaaac ttccagaaac tataaacaca aaatacgatg aggcttatcc
     1561 ttttttaagt gaagatggaa aaaccttgta ttttgcatca aaagggcata atagcatggg
     1621 cggttatgat atttttaaaa caaattggga taatgaaaat aatcaatggt ctgagcctaa
     1681 aaatttagat tttccaatta attcgccttt tgatgatata atgtatgctg ttgataaatt
     1741 tggagaaacg gcatttttta cttctacacg cgaaagcaaa acaaatgaat gtggagtgtt
     1801 tagaattttg gtggaaacta accccaaaaa acgcgaaatg cgtaatatag atgatattta
     1861 tttaagtgca aacccaagtg taagcgaatt ggctattagc gaactgagta atagacagaa
     1921 aactagaact gaattaagcg atacaattat tataaaagat gtacaaacaa tagaagttaa
     1981 aaatttagaa tctgatattg atttagcaat tcaggaaatg tttgcaaaat tagacgaaat
     2041 ttctggcgaa gtaagtgaat ataaaaagta tgcttctact gcaaataaac tttcacagga
     2101 aagtctaagc gaaattaaaa aaataaattc tgaagtttca aaattaaaaa cagcaaacgc
     2161 aaaagctaat gaggcaaaaa tccaagattt acaaaaccaa attaatgatt taggagatag
     2221 ggcaattcaa ttaaacgaaa ttgctaaata tttttatgat ttttcggtta gaacagaagg
     2281 tttgttaaat tattatttgg cagaagtaaa agtgttggat aaaacttcaa gaaatgaaaa
     2341 aagtttagag aaatctgtat atctaactca agcagaaatc aataaaatag atagaagttt
     2401 tcctttagat aaatatatag acgagctgaa caaaataata aattccgaaa atgataaatt
     2461 agcaaaatat gataaattaa ataaaccgct tttttctgag ttggagcagt taaatacaaa
     2521 aattactaca aaaatagaag tagctaaaag cgagacagaa tttgaattgc gagaaaaata
     2581 tatttatgac attaaaacct atgaaaatca aaaaattgac ttaattgata aaataaaaga
     2641 aaatcaaatt caaattgagt ttgcaaaatt cgacattgca gaaaacaata aaaaaatagc
     2701 agaagcaaat ttattacttg aagaaataaa gggaaattta tttttagacc caaacttgaa
     2761 tttatcaaaa gcaaataatg atatagaagt gcttaaatcg cacaagtcgc aaactaatct
     2821 taaagaatta ttaaatgttc aggagagtat aaataccgac cttagtctat attctccaat
     2881 tattgattat gatgctattt tgtatggcga agatttggct aataatgaag ttgttattga
     2941 taaagaagaa tatatcaata aattttcaaa ttatatttca aacgaaacaa aaacttttgg
     3001 agaaagcctg attacaaatg attctttaaa aaatattatt gcaaatttgg aactagattt
     3061 tgataaaaca gaatctcctg aagaaaagca aaatataata aacaatataa atagcacaca
     3121 aattaagatt aacgagaata aaaacgtatt aagagaaaat tctaaaaata ttagccaagc
     3181 tagcaatcaa aaaaatataa gcaacttaga agtattgaaa gcaaaagatg aagaatcaaa
     3241 atttaaaagt gaaattgccg aaatacaaaa tttaatttct atttcttctg aaattgaaga
     3301 agatatagct gattttcaaa tattaagtgc taatgacgaa agtcttgatt tatggctttt
     3361 aaaattaatt aaaaatgata ttgaccaaaa tgccgaaata aaaatttcag aacttgaaaa
     3421 tcaaatgaaa ggtgaagtgc ttcaaattca gtctattatg gcagaaatga ggcaaaggct
     3481 atcaagtaat caaaatagga taaatcagtc taagccaata cttgcagaat ttgaaaaagc
     3541 aaaatccttg tttgcagttt ctgaaacctt aaataaaact caagaaaaaa atcagttgat
     3601 aagtcaagcc aatggaatta ttgaagaaaa tttaaaaaat tctattgatt atctaaatac
     3661 ctcgcttcaa gatttttatt cagattattt gttgtactca gccaatatta aaaaatatga
     3721 tttagcagat aatagaactc aatcttattc gctaaaagct gaataccaca aacaaattgc
     3781 agatagtctt caaattttgg cagaagaaaa tagctcagaa tcaattagtt tgcttggcga
     3841 ggcttataaa aatattgaaa ttgctaatct aaatttgaat tatatttttg aatttttgga
     3901 aaatggagct aagtatgtcc ctaataaaaa ctttggcaat agacttgaaa ctttaaattt
     3961 tattaatgaa attactacaa ttaaaacaaa agaattattg cctgaagaaa ttgaagttat
     4021 tgttgaacta gaaccaaaaa atatagaaga attaatttta aaatctgaac aaacaagtaa
     4081 tgaaattcaa gctcttacag caaaatttgc aagttctaac gatgaggaaa aagatgaaat
     4141 tttaaaagat atagaaaaac ttaatcaaga cataaaatta caacttaaaa caataaatga
     4201 ggaaataatg caagttcaac aacgaactat aaatgagcaa cttgcagaaa ttgtttcaaa
     4261 taatccaaaa gctgaaattt ctgatttaaa agaaaaaata aaacattaca ctgacgactg
     4321 caacacttat tacgaaactt acgattatta tttgttaagt gaagttgtta tcagaggtgc
     4381 aaatgtttta aatcagcaaa atgaattgtt gaaaaacaag aaagtaatta ttcttcctca
     4441 atctcgtatt gataatttaa ttgcaaattc agctaagttt gaaaattttg tggataatga
     4501 aagtcaaatt ttggcagaaa ataaaaaatt aatcgcagaa caatctgaaa ctaaagaaca
     4561 agaaaacatt attcaatccg aagtgattga aaataaagtt gatacaacag aggcgagtct
     4621 tgcaataaac gaaccacaac cagaaatcgc tgtcactatt tcagaaacag ttgaacctca
     4681 agctgaaata gttgaaaatc agcctgtaac gagtgagact aagtctgaaa taattgaacc
     4741 taaacctgaa atagcagaaa ctaaaactga acaagcaggc gaaagttcgc aaactcaaga
     4801 gattaagcca agcgatacag aaagagattt agttgttgtt ggcgaaaata ttgaagttga
     4861 aaaacaaaca tataatttag atgagtttgc acaatattta agttcaaata caggagattc
     4921 agaatttttg tctaagaata ctgaaataaa taatacccaa aaccaaattg acaaaaatca
     4981 aacaaaaatt ttagatttga ataataaaat tgagcaaaca aattctcaaa aacaaaaatc
     5041 taaacttatt tcagaaaaat caatattaga aagtgaacaa actaaaatct tgatagagca
     5101 aagtcaaaat aaattgagtt ttgttgagga agtaaatgaa acttatgcga agacaaaccc
     5161 tgaattgata aacaactcag aatatcaaaa gctaattgaa aattataaag ataatagaaa
     5221 ttctgtaatt tcttatagta atttttactc taacgaagaa cttattgaaa tttacgaaaa
     5281 ggcaaacgaa acagaaaatt taattctttc agaaattgct aaaaatttga aatctcaagg
     5341 ttttgttgag ttagctaaac tcgaaacaag agaaattgca agcaatgaac taaaacctga
     5401 aaatgagatt tcttatcaaa ataattatga agatgaaaaa actttgaact cattgcaaga
     5461 caggcttgaa aaactcgaaa tcagcattaa aaataatgaa aatcaaattc aagagtataa
     5521 taagttaata tctgaatctt ctaaaaattc tcaaattaaa aagcacaaaa aatcaattaa
     5581 caaaatagaa aaacagcaag ttaaactcat taaagactat gcagaaacag ccaaaaatta
     5641 tgagatatta acttataata caagaacaaa atattttgat gagcatttaa aatcagaaga
     5701 tgaaattata aatactgttt cggatagttt gaaaactcaa tctgaacaag attttgaaga
     5761 attaatttct atttattata aacttttgag ttttgattca aaagtagaaa accaaactgt
     5821 ttttgaggaa tttgaaaaag ctaaaaagct ttcacaactt agctttaaaa aaatagaatt
     5881 agctaacgaa gttttaagtt tccaagataa aaatactgaa gtcttgatgc catattacaa
     5941 gcaacttgtg attgaagatt tggctgaaaa taccgaaacc aaaacaagtg tggaaactta
     6001 tgtgcctgaa aaagaggtga ttgcagacag tcaggaaaat gttgaggaag aagtgataca
     6061 agttagagaa aaacttgacg aaattgctca ggaattagaa acttatgatt tttattatcg
     6121 cattcagttt gctgcttata atgaaatggt tggtgaagaa aatttcccaa ctttattgcc
     6181 attatttacc gagcgcatag aaaattctcg cttgatacgt tttatgacag gcacatattt
     6241 tcagttggca acagctcagc aaaatcttcc aagagttaga gccacaggat ttgaagatgc
     6301 atttattgta gcttatttta atggaaaacg aattactatt gccgaagctc gaagactaga
     6361 acaagaattg caagttcaaa aacaagaaga acttttggtt agtaaagatg aaaccaccac
     6421 agaaacaaca aatgctgaaa tagaaactga tgaaactttt gtaacacctg ataagactgc
     6481 tgagcctgag cctgtggttg ccgaaacaag aactacaatt aatacagaat tagtacaaac
     6541 tacagatgtt ttctattgca ttcaagttgg agtttatcgc gaaagaatta atcctgaaag
     6601 gctttataat ttagctcctt tgttttatga tgaatataga cctggtttgg ttcgccatgt
     6661 ttttggaaaa tattatgatt taaacactgc acttcaagag caaaacagaa ttagacagct
     6721 tggaattact gatgcttttg taactgctta tgctaatgga aaacaaattt atttatacga
     6781 agccagaaat ttattagcaa acgaaaatct tgaaaataaa caagaaatta ctttgtctga
     6841 agagcatgtt gcacaagcac aggtacaagc acaagagcgt ccagaaatgc agagagaacc
     6901 aagagaacca gagcaacaac aagaagtagc taagatagaa tattatattc agttgggagc
     6961 ttttaggaat gaaccaaata atttagttag agacatgttt ttgaaattag caggaaataa
     7021 aaaactttat aaaactaata gtaataattt acttatttat agagtcggca gttttgataa
     7081 atacagtgaa gtacttacgc aattaaataa tattaaaaat ttaggtgtaa cagatgcttt
     7141 tgttttggca ttcaataatg gtaaaaggat taatttaacc actgcaagac agttagaata
     7201 agcacaaaaa ttgcatcaaa ttaagaattt ttatattttt gcaaaaaata atttggaatg
     7261 aattttattt taaacatcga aacctcaact gatgtttgtt cagtttcact atcgcaaaat
     7321 ggcgagacta aatttttaaa aattcataat cctaatttgg agaatggcga aaaagcctct
     7381 cattctaaac ttttagcaag ttatattcaa gatattttaa aagaaaattc tattttgcca
     7441 gaaagcatta aagctgttgc tattagtggt ggtccaggtt catatacagg tttaagaatt
     7501 ggtctaagta ctgcaaaagg atttgctaca gctttagatt tgcctttaat tgctattgat
     7561 agtttacaaa ttgttgcagc tatggcaaaa agcaaaatag atttttctta tgactatata
     7621 attccaatga cagatgccag acgcatggaa gtttataccg cgatttttga ttcatatttg
     7681 aataaaattt cagaaacgga agcaaaaata attgatgaaa attcattttc ggaattttct
     7741 aataaaaaac ttgtattttg tggcaatgga gcagaaaagt gtaaaactgt attaatgtca
     7801 gaaaatttta tttttcttga cgatattttt ccttcagctg aatttatgtc agatttttct
     7861 tataaaaaat ttatcggcaa agaatttgtc gatttagtat attatgaacc tttttattta
     7921 aaagattttg tggcaacaac gcccaaaaat aaaataattt aaaatttaat gttatgaaat
     7981 ttacaagcac atcaaatcct gttctttcag aaaaagcatt tgaagctctg tccggtgaag
     8041 gcactatgag cataaaaggc actttaaaca aatctataat tttgtttttg ttattactag
     8101 tttctgctag tttttcatgg aaattagcta cttacgacct tgaacttttg tctcctttaa
     8161 tgtggggagg agcaattgtg ggatttattc ttgcaatggt tacaattttc gctaaaaaat
     8221 acgctaagtt tacagcaccg ctttatgctc tagctcaagg gtttgtctta ggctcgattt
     8281 ctatggtgta tagctcactt tattcaggaa tagttttgca agctgtgctt attacttttg
     8341 gaataatggc agtgatgcta gtattattta gatctggaat tataaaagtt acaaataagt
     8401 ttcgctctgg aataattatt gccacaggtg gcatagctct tgtttactta gtttctttca
     8461 ttttaggatt ttttggaata caaattcctt atttgcactc tggcggtcca ataggaattg
     8521 gaattagtat atttataatt ataattgctg cattaaattt aattttggat tttaatttta
     8581 ttcaagaatc ttgtgaaaga ggagctccta aagaggcaga atggtttgca gcatttggtt
     8641 taatggttac tttagtttgg ttgtatttag aagttctaag acttttaagc tatttgaata
     8701 aaagataatg tttgagattg ctattgttac agaaaaaaat gaaaaagatt tttttcaagc
     8761 ggctttaaat atttatgaaa atgatgaaaa ttgggttgct cctttgaatg gagatatact
     8821 tggttttttc tattcaggaa ataagccacg agaaaaaaaa tgtaattttc aattatgggt
     8881 tttgtataaa aacaaaattc cagtaggcag aatttctgca tttttaccag taaaaaaaca
     8941 taaagctaac gatattaaag ctggtggaat aggctttttt gattgtatca attctcaaga
     9001 agcagcaaat atgctttttg atgtagctgt tgattggtta aaagcagaaa atatgcaagc
     9061 agttgacgct aatactgttc ctggtgaaaa ctttaatcac tggggagtat tggttgatgg
     9121 atttatacca caagcttatg gaatgccata taataaagag tattataagg aacttttcga
     9181 aaattatggc ttccaaaatt attttgagca atattcttat catgttgatt tacaaaaacc
     9241 ttttcctcaa cgacatattg attttgcaca aagggttata gattcaggcg actttgactt
     9301 taaacattta gattttaaac aaaccgataa atttgttgat gatgtaacca cagtttttaa
     9361 tgaagtgtgg tcatattttc atgcagatca tactaaggtt gaaagaaaag aatttgaaaa
     9421 aatgtttaag gcaatgaaac ctattgtaaa tccaaagttt gtgtggtttg tttataaaga
     9481 tggtcgccca ataggaatgg aagtttgttt gccagactta aatcaagtta ttaagccttt
     9541 taaaggaaag ctaaatttat ttaattcatt gaaattagtt tcaaaaataa aaaaggttaa
     9601 acgagctaga gtaattgtgt ttggcgtgca tcccgattat caaagaattg gagtaactca
     9661 tgctttgtat gtgaaaatgc ttggcggttt acgagaagct ggagtaactg aattggaaat
     9721 gtcatgggta ggcgattaca accaagctgt aaatagaata tacagatacc ttgggaattc
     9781 tcatcttgca aaaactcata taacttatag gtatatgata gatgaaaatt gcaagtttga
     9841 aagatttaca aacgaaaaac aagaaaatca gtaatattgt attgtagatc tgaagttatt
     9901 tgttcgttag tacaatacaa gactatcaaa ggcttgtccg taggacaaca tgtgattaac
     9961 cgtaggcggc agcctgcggt ggtgatgcca tcaaaagcct tgcccgcaag ggcaacacaa
    10021 aatcccgaat gaggaatgaa aattacacac tttttaggac actactatag tttgtgtttt
    10081 attttgtgat tttagggtgc tacaacgctg aaaacagaag caaatctatt atattgtgga
    10141 tttaactttc tagatgaata aaaattccat ggtctatagt gtttccatat tttgaaagac
    10201 caaaaagttt ttcgtttaat gttgttttat gatttggaaa attctcttca atcattgtgt
    10261 ttatggcatt ttcgctaagg ctatgactgt aggaattcat tattacaaaa gatttttctt
    10321 ctaagagaat agaaatattt ttaagaagtt tatccaacat tttatttaat atccattttt
    10381 cattatttgc acctacacct attggtggcg ggtcgagaat tactgcgttg tatttattat
    10441 tccttttaat ttctctttca acaaactttg gagcgtcttc gcaaatggct cttatattgt
    10501 caaagcctga atttttagca ttaagttttg cccagtccac caccttttta atggaatcta
    10561 tatgagtaat ggtttcagaa aatggagcag cggcaatgct tgttaaacct gtatatccaa
    10621 ataggtttag gaatttcata ttttggtaat ttttagaaat agatttaatg aaattccaat
    10681 ttaaaacttg ttcgggaaaa atgccaatat gttttgattg agttaagttt aagcaagcct
    10741 taatgtatat ttgttcatct ttatattcaa tataccacgg tttttcaaaa tctttaaaaa
    10801 tattccaata acctgagttt tttgaagttt caataaattc tgcatttgcc atatctttcc
    10861 aatctttata agtgagatga ggcttgtttg atgcagttat ttctggtcga attaaaacaa
    10921 tatttccaaa gcgttctaat ttcttaccat tgccacaatc aattagctca taggaaggaa
    10981 aaatatttgt aaaatttatt tgcacagaat ttatttaaaa attgtacgcc cattgagcgt
    11041 agtcgaaatg agcgtacaat tttcacattt taattgataa aaaaacagtt taatcaattt
    11101 atttctacaa gagcaatcac atcatcttta tcaacaagat caccttgttt aatcaaaaca
    11161 gaagctaatt ttccattaaa atctgccaga atttcttgat aatgagtgtt agactctagg
    11221 tagaacaagc gtttcccttt agtaatagta tcgttaggtt taataatttc tctgtcaagt
    11281 tcaacgctca aatcgtaata aactttacct ttatcaggag attttatttc tactaaattc
    11341 ttttcaacct ttgtagcatc aggagttatt gatttgtttt cagctttttt atattttaaa
    11401 gaatttagtt cttcttcaaa tctttctttt gctaaacctg atttataatc gcgatattga
    11461 cgctcgtgca ttgcaaattc gaaaagttct tcatcatctt gtcctaaatc ccagccattt
    11521 tctatcattt ctgcacgata tttatctaac tcatctggat atgcttcttg aggtacgcct
    11581 gtgtagaact ctttaccttg agcttttgca atttcgataa tttctggagc aagaggtcct
    11641 ggtaaaatac ctgctttgcc ttgaatcata tcccaagcgt ttttgtcaat catttcaaat
    11701 ctatttttac cttgaagcat gagtattaca ttcatcaaag aaatgttttt cacatattga
    11761 ctaaatggag ttactaaagg tgggtatcct aatttgggcc aaacatattg aacttcgtca
    11821 aatagcataa ccaaaacttc atcggttgtt aattcttttt taccttgagc ttttaattgt
    11881 gcatttattc cagtatgaac atcttttaag tctgccatca tagagcccat cattccgcca
    11941 ggcaagcccg aaccaataag tagagctgtc atttctctat tttttggatt tatccataaa
    12001 ccaagccatt cgtcaataaa ttcttgagtt aaacttcttg ccttcatata agcgttcata
    12061 tttatgtttg gaactttaaa tccagcatct ttaagcattt cttggatagt tatcacatca
    12121 gggtgagaag ttccccaaga aagtggttcc atagcaacat caatatagtc agcaccagct
    12181 ttggcaactt ctaacattga agctacagaa aatcctggac cagaatgacc atgatattga
    12241 acaattacat ttggaaaaga agctttaatt tcgctaacta aacgacctaa agttactgga
    12301 cgccctatgc cagccatgtc ttttaagcaa atctcctctg caccgtattc tactaatttt
    12361 tctactattt ccatataata ttcaacagta tggattttag aatgagtaat acttaatgca
    12421 gcttgagaaa tcatgccagc ttcttttgca tattttacag atagttcaag atttctatgg
    12481 tcgtttaaac cacaaaacga acgacagata tcaactcctt gtgctttttt aactttgtac
    12541 attaaacggc gaacatcagc aggtactggg aacattctta aaccatttaa agctcgctct
    12601 aacatatgag tttgaattcc agctttgtta aatggagctg tccattctct aaccgcttgg
    12661 ttaggatttt caccataaag taaattaact tgttcaaaag cacctccatt ggtttctatt
    12721 ctgtcgaaac aacccatttc aataattaca ggtgctattt tttttaattg atctacgcgt
    12781 ggaacatatt tgccactcga ttgccacatg tctctgtata aaagactaaa ttttatttct
    12841 tttgccattt tctattaacg tttttttcaa aaataacaat tatttttgga taaaaaaaac
    12901 gaaaaaggtc ttcaattata tgttattaaa gacctttttt gtgctattat actaattact
    12961 gattattttg taacaacaac cctttgaaca gcaataatgc catttttatc tctaagattt
    13021 acaaaataaa tacctgcttt aagatttttt attacagatt tattagatgt catctttgat
    13081 tctaaaatca gacttccttt tatgtcagta atatttatag aaatatcatt gaaatcgtcc
    13141 tttttataat caatagtaaa ttcatttgtt gtaggatttg ggaatacagt aaagatgtca
    13201 tcataaaatg tttgtggaac ataattgcca gtatccaatg caatatttac aactccaatt
    13261 ttgctacagc cgatagcatc agtaacttta acttgatacc atccttcgca taagccaatt
    13321 tgagtttcgc caaaatttcc aataggactt tcccaataaa aagtatatgg atatactcca
    13381 ccattagcaa aagcagtagc tatgccgttg cattcgccag tgtcatcaag tgtttgagag
    13441 cttatttcta tttgagaata ttgtactttt acttcgtcat atttatattc accacagcca
    13501 tcaaagattt ttacccaata agtagtttct ccgacttgag gcgttacagt tattgaagga
    13561 gttgttgctc cagtactcca tgtgaacatt gggttaggtt ggttagatac agctgtaaag
    13621 gtaacgcttt caagagtttc gcatgatttt acatcatctt ctgcaattcc tagtgctaaa
    13681 tcaacaggcg gaggatttgg taattcaatt tgatagcgtt taaagcaacc aatcatatct
    13741 ttcacttcta ttctataatt gccggggcac aatccagata gtacaggatt tgttgtagta
    13801 tggattgaat ctgggaagaa atgataaggt tctcgagcat atttatatgt gaaaggacca
    13861 tcttcaccat cgacaactaa ttcaattatt ccatcgcaag cattgtggca actcgggttg
    13921 ataatgtttg cgttggatag agttagtccg tttactagaa ttgtaattga gtcaagtacc
    13981 tcattatcac attcatcaag cattgtaagg taataggttg tgcttccaag ctgtgggtat
    14041 atagtaatag aatttgtatt ttctcctgta ctccaatgat aatcgatgaa tggatctcta
    14101 ttacattcgc caactaaagt tatagtggtt ggagcttctt ctccgcaata aaaagcagtt
    14161 tcaatatctt gaattcctcc ttttataaat tctgcatcgt atatccaaat tgtgtcataa
    14221 attgccatag attggtttcc acaaggacaa gatgtgaaaa aagcaataat aattgattct
    14281 gttccttctt caattccatc attaaaagct gaataataaa tagtatctgt catttctcca
    14341 gcaggaattg taatagattc tggaaatgga gtaaaatcaa caccttcggt agcagtactt
    14401 gatgggtcta ttgccatctc aatagttaaa tcttggtcaa ctgcaactcc ctctgctcta
    14461 gaaataacat aataatttgt acaaccttcc cataagtcat tttcgtctcc atgttgagca
    14521 gtattagcaa ccgatacagc accaccggag ataaaacttc cagcttctaa aaatacacca
    14581 gagtcatatg aagagtcgct acgatcgcct atagctaatt taatatggta agtttggcaa
    14641 gcttgtactt ctgctctagc agttagcaaa atagtgttac catcaaactg aaccgcacca
    14701 tagtaggaac ctggagatgt tgagcctgca ttaggttgtg cataataata attatagtta
    14761 tgtacattat ttatagtaac tggcatatta ttaggcaaaa gagcaatatt aattgcatta
    14821 tttgtgtaag gaccatttat gccaggacca gataggaaaa acccaaatac gtcattaaaa
    14881 gaggaatttg catactcagg aaactcttca gagccaaaaa tataattaaa tacaatagaa
    14941 tcacttgctg gaataaagtc aaattcaatt acgcaagcat cgtttatgtt tgctccagaa
    15001 accaaagcag ctaaatcagg gtcagaacca gagcctggcg atgcacctga agatgaagta
    15061 gatgtgttag gacctgcagc ggctgatatt gcgcctgttg acattacaat ccctgactta
    15121 aaaggaaaat gtggattacc aggatttcta aaatatccat aagcaccatt atttgctgtt
    15181 acattagaag cttgaacgca accatttatc aaagtgtctt gaattaatgt tgtaacaggt
    15241 attccacttt gagtaataat tggacttgtt tgtgaaaaca aaaaagatac actaaagatt
    15301 aaaaataaag atagtaatgc agtttttttc ataatatata tattttatat aaattgtaaa
    15361 gttacaaaat gttttataaa tgccactaaa atatgacggt ttttttcaca tttggttgtt
    15421 tatttttaaa tttcatgtta aatttgccaa taattatgaa aagataccta gcattagata
    15481 ttggacgaaa gcgaactggt attgctgtta ccgataccgc tagaataatt tcatctgcat
    15541 tagaaactga agaaacacac aagttagaag agtttttaaa aaaatatttc gctgaaaacg
    15601 aagttgaaaa aattattgta ggaatgccaa aacaattaaa caatcaacca tctgaagcgg
    15661 ttaaatatat tgtacctgtt ttaaatagac taaaaaaagt ttttaaagaa tatgaattta
    15721 ttgaagttga cgaaagattt acatcaaaaa tggcttttca aacaatgata gatgcaggaa
    15781 gtaaaaaaca acaaagacaa gaaaaatcaa taatagataa aattagtgct gcaattatat
    15841 tgcaatcata tttagattca gaaaattata aaaagaaatg atattaccaa tatatttata
    15901 cggacatcca gttttaagga aaaaatcagt tgatatttct aaagattatc ctgatttaaa
    15961 acagcttatt gaaaacatgt ttgaaacaat gtacaaagcc gaaggtatag gtttggctgc
    16021 accacaaatt ggagaaaata tcaaccttgt agtagttgat gcaagagttt tagcaaaaga
    16081 tagacctgag cttcaagact ttatacgtgt gtttataaat ccaaaaataa catgtaattc
    16141 cgagcaaaaa gtaacagtag aagaaggctg tttaagcatt cccagtattt atgaaaatgt
    16201 aaatagatgt gaaagtttga atgttgaata ttttaatgaa aatttcgaaa aagtaactga
    16261 aactcttgac ggacataaag ctattgtggt acaacatgaa tttgaccatc ttcaaggaat
    16321 tgtgtttact gatagaattt ccccaatacg ccgaaaaatg ctacaaaaaa gattaaataa
    16381 tattgctaaa ggcaaggtag gagtttcata taaagtaaaa atcgcttaat taaaactaaa
    16441 tattatgaaa attaaatgtt tttttattct gataattatt gttggcacat tgttttcttg
    16501 tggctcaaaa acaaagtctg atacaaaaaa tgaagatttg aaaaatcaaa ttgtagaaaa
    16561 agaaaaggaa acatacagtt ctgatagtct taatcttgat ttggctaacg atttgataac
    16621 tatgtattta gcatatgcta atgaatttcc tcaagatagc ttggctcctg aatacctttt
    16681 taaagctgct gaaattagta tgaatattaa caaagctaaa aattcaattg attatcttac
    16741 tgaaattgaa aataattata aaaactatga taaatatgta gccactcttt ttttaaaagc
    16801 ttttattttg gaaaactatt taaagaattt tgaagatgca gaaaaatatt attctattgt
    16861 agttgaacaa tatcccgaac attctttagc tcaagatgct gaagccgcta tatctttttt
    16921 aggaattgat gatgaggaat tgataaaaat gtttcaacag tcgatgtagg ctcaagctat
    16981 gtacgacata ttgataacta gccttcgtaa aaatccattt ataaggattg taattccttt
    17041 tatcataggt attgtaataa gtataaattt tactttttct tcagttatta gcttatcaat
    17101 ttttgttgct tctgcaattg cttttgtagt atttgcattg atagaacgga aattttcaag
    17161 aatttggata aatggagttc ttataaattt aattttcatc tcttcgggaa tgtttctaac
    17221 tcaatcgctt aatgaaaaag ctgttgaaag tttaagttca aataaacaag gcaatttaat
    17281 tggcgtaatt aataaagagc caaaaattat ggaagcgtca acaaaagttg agctaaatat
    17341 aattgcattg aaaagtcaag accaatggtt agaaacaagt ggaagtacaa ttttatacat
    17401 tgaaaataat gaagatgtaa atagtttgaa agttggagat aaattgattt ttagtcctga
    17461 tttgcaagaa attggaaata aaggcaatcc cgaagagttt aattataaaa aatatttgag
    17521 ctataattta attttaagta ctgattattt gagtagttct gaatggaaaa tcttaaaaga
    17581 aaacaaaaat ataaaacttc aacataggtt ttcaagattt agaactaagc taatagattt
    17641 attaaaagcc aataatttac acgatgatga attggctgtg gtttcagctc ttgctttagg
    17701 ctatcaggat aatataagtt atgaattgcg tcatgcttat tcttcgtctg gagcaatgca
    17761 tattttggca gtttcaggaa tgcatgtcgg aattgtttat ggagtaataa cttttctttt
    17821 atctttttta aaatccaaaa aattacgtat tcctaaaata tttttatcaa taatattggt
    17881 ttgggcttat gcttttttaa caggtttgtc gccttcggtt tcgcgtgctg cacttatgtt
    17941 ctcagtagct gcgttaggaa atatacaaaa gcataaatca tcttcactaa atgcaattgc
    18001 tgcatctgca tttattttgc ttttaataaa tccatacaat ttacttaatt taggatttca
    18061 actttcatat atagcggtaa taggaataat tttgctttac aaacctattt acgaaatagt
    18121 aaaaccaaaa aataaaataa ttaatagcat ttggtctatg acggcagtat cgcttgctgc
    18181 tcaaatagtt acagcacctt taggaatgta ttattttcat caattttcaa attatttttt
    18241 aataacaaat tatttactta ttccaatttc aaccattgct atttggttga ttattacact
    18301 ttttgctgtg tcatttattc cgtttttgtc tgcttttatt gcaaaaattt tagtctatgt
    18361 cataaaagca atgaattatt gtgctttggg catagaaagt ctgccatttt cggtaacaaa
    18421 tgatatttat ataaatttac ctcaattaat tttgctgtat ttaattataa tttttgtttt
    18481 tctgtttttc ttccaaacta agctatatcg acatttggtt tgtgctataa gttttataat
    18541 aatttttctt acaattggct tgtttaaaga tatagaaagt tctaagcaaa aatattttat
    18601 cgtttataat atgaataaaa acacagttat aaatattgta aatgctaaga aaaatatagt
    18661 ttttgcttcc ttagacgatg aacttgaaca aaatattgaa tatactgcca aaaataattg
    18721 gttgaaaaaa ggtttggaac atcaaaagta tgttgatata agctccaaga gtaatttatt
    18781 tttaacaaat ctgctttcaa tttcagattc agaaattttc tataaaaata actttatata
    18841 tttttcaggt ttaagaattt atgtcttgaa cgataatttt aaaatgttga aatccgatga
    18901 ggaatttaaa aagttagata ctgattatat tgtattatca aataatccag aaataaaatt
    18961 atctgaaatt gctgaatttt ttaatttcga tgaaattata attgacgcat caaattataa
    19021 aaataaaaca gaaaaatgga tagccgaaaa taaagactta aattataaat tatttgatat
    19081 tagagaacag ggagcttttg ttttgaaaat agaatgaaaa gaggctaata aacattttta
    19141 aaattcctta attttccgtt ctttggactt aaattttttt gatgcaaaac acaaataaaa
    19201 tatcacttag aggcgtaaga gtcaataatt taaaaaatat tgatgtggaa ataccacacg
    19261 ataaattggt tgtgataaca ggtttatctg gttctggtaa gtcgtcttta gcttttgata
    19321 caatttttgc agaagggcaa agacgctatg ttgaaagttt atcatcttat gctcgccagt
    19381 ttttaggtag aatgaacaag cctgaagttg attttattga tggaattccg ccagctattg
    19441 caattcaaca aaaagtaaat acacgaaatc ctcgctctac tgttggaaca agcacagaaa
    19501 tttatgaata tttaaaactt ttatttgcaa gaataggaaa aaccatttcg ccaatttcgg
    19561 ggaaagaagt gaaaaagcac aatgttgcag atgttgtcga gtttattgtt aatcaagaag
    19621 atggaactag agttttgatt agttttactt acaaatttaa ttcagaaatg agtaagacag
    19681 aacaacttgc attgctcgaa aaacaaggtt atgcaagagt ttactgcaac gataaacttg
    19741 tcaaaactga agatgtatta aatgaaaatt gcaaaggaat ttctaaagaa atagatatta
    19801 taattgaccg tttagttatt cgtaaaaatg atgatttgta tgcaagaatt gctgattctg
    19861 ttcaaactgc tttttttgag ggaaattatg aatgtaaagt aattatttta ggtgaagaca
    19921 aaaataaagt ttcaaaattt tctaatcatt ttgaagctga tggaattaaa tttgaaattc
    19981 ctcacgagca tttatttagt tttaacaatc ctcttggtgc atgtccgctt tgcgaaggct
    20041 ttggtaaaat tcttggtatt gatgaagatt tagttattcc taacaaaact ttgtcggttt
    20101 acgaaggagc tgttgcttgt tggaaaggcg atgtgatgca acaatgggaa aaagaattta
    20161 ttttacgggc tggcaaattt aattttccaa ttcatcgtcc atattatcaa ttgagcgaaa
    20221 aagaaaaaga ttttttatgg aatgggaatg atgcggttta tggaataaat gattttttta
    20281 gttttttgga atctcaaaag cataagatac aatatagggt tatgatttcc agatttagag
    20341 ggaaaactaa ctgtacagaa tgtaaaggca aaaggcttaa aaaagaagct ttttatgtaa
    20401 aaataaataa tcattccatt ggcgacctta tagatatgag ttttgaagaa ttaaaagttt
    20461 ttttcaaaaa tttaaaactc tcaaaagtag aaaaggaaac agcttcacgc ttacttgaag
    20521 aaattacttt aagaatagat tctgtgcttg atgtgggatt gccgtattta acattaaatc
    20581 gtctttcttc aactctttcg ggtggtgaat cgcaaagaat taatttagct acaatttttg
    20641 gttctggttt ggtaggatcg ctttatgttt tagacgaacc aagtattggt cttcattcgc
    20701 gggatacaca aagattaata aaagttttaa aaaatttacg cgacagagga aatacagttg
    20761 ttgttgttga acatgatgaa gaaataatta gagctgctga ttatattatc gacattggtc
    20821 cttatgctgg cgaaaatggg ggtgaagtgg tttttgctgg agatgtgaaa aaaatgttgc
    20881 agtcaagtaa tacgcttact gccaagtatt taaaaggaga attaaaaaat ccaattcgcg
    20941 aaaaacctag tcagtggaga aattatattg aaattattgg agccagacat aataatttaa
    21001 aaaatattaa cgtcaaattt cctcttggtg taattactgt cgtaactggt gtaagcggtt
    21061 ctggaaagtc tagtttagta aaaggaattt tatatccagc tttaaaaaaa caattggggc
    21121 attttgctga tagaaccgga gagcatgaaa gtcttgctgg cgattacaaa ctttgtagcg
    21181 atatagaata tgttgaccaa aatccaatcg gaaagtcatc gcgttcaaat ccagcaactt
    21241 acataaaagc ttatgatgaa attcgtaaac tttttgcaga acaacgtcat gctaaagcaa
    21301 atggatataa agcttcacac ttttctttta atgttgctgg tggacgttgt gaaacttgtc
    21361 tcggtgaagg cgaaattact gtcggaatgc agtttatggc tgatgtccat ttagtttgcg
    21421 atgcttgtaa tggaaaaaaa tttaaagaag atattttaga agtaaaatat aagggcaaaa
    21481 atatttatga tattttagaa atgcctatta gtgaagctgt tgagtttttt ggctcggaaa
    21541 ataaagctac agaaaagaaa attgcaagtt tattacaaaa ttatttagat gttggcttag
    21601 gctatgtaaa acttggacaa tcttctaata ccttgagtgg tggcgaaagt caaagaataa
    21661 aacttgcaag ttatttaagt atggaaaaag caaatccaac aatttttgtt tttgacgaac
    21721 caactactgg gcttcatttt tatgatattg gaaaactttt attagctttt gaaaaaatgc
    21781 gagccaaagg gcatactctt attattgtgg aacataatcc agaagtgatt aggtttgctg
    21841 attggataat tgatttaggt ccagagggtg gcgataaagg tggtgaaata gtttttgaag
    21901 gaacaccaga agacttgaaa ctttcgggaa cttatacagg aaaaagttta gcaaaataat
    21961 ttatctcaat aatgtaacat ttcctttatg agaatattct tcgccattca aacagcgagc
    22021 gcgtaaaaag taaacataga catcgcttgt tagttgctca cctctatatg ttccgtccca
    22081 accttttgtc catatttcgt ctgtttggaa aacaatattt ccccaacgat cataaactac
    22141 aaaaaatact tctgtaacta aaccatatgg gtagtaaggt ttgaaatagt cgtttttgcc
    22201 atcgttattt ggagtaaacg catttggaac gtaaatataa ggatcgccac aaataacttc
    22261 tttcacataa atttttatag aatctctaat cctacaacca tgcaaatcgg taaatttaac
    22321 ataaaacatt gttgtatcat caggtgtagc aataggatta gaaatttcta tattagataa
    22381 aaattttgaa taatcccata aataataccc tgaacttgat gaaagtgcaa gtaaatttac
    22441 agattgtccg cgataaataa ttgtatcact tgcagcaaca gataaatcag gaatataatc
    22501 ttggtttatt attgtgaaat tcctagttaa tgaacaattg tttttatctt ttatagttag
    22561 ttcatactca tcttcgcata agttttcaat atttgctttt tgcgaaccat ttgaccacga
    22621 ataatagtaa ggtggcgtgc ctccaatcaa ttctgtatga atactgccat tacaaacacc
    22681 tgtacacagc atatttgtta cgatattatt catttctaat aaaggtggtt gagttaattc
    22741 aatttcacta acagttttac aattattata atctaaaaca gtaacttgat aaattcctgc
    22801 agaaacatta tctaaagatg tttgtgtact tccttggctc cagttgtatg aatgaggata
    22861 tgtgccacca attgcagaag ctgagacaga accagtttca ccaaagcatt ttatttcttc
    22921 gcttatttgt gttgttacca gtaactctgg aggttcatat atgtattgat attgatgagt
    22981 tacacaattg ttggcatcaa ttactgttaa tgtataaact ccttggcaaa gttcagtaat
    23041 ggaagtactc gtagaatcat ttgaccataa atacgaatag ggtggagttc ccaaactaac
    23101 gctggcgtga atttctccat cgcaaaatcc atagcatgaa atatgaacaa cattacttaa
    23161 acttgaagtt aaatctgaag gagaagacat aaaaacagaa tcaactcttt ggcaaccatt
    23221 attatctgtt attgtaacat aattttgtcc tgtaaaacaa gtgttgttag ttgcattact
    23281 tccgcctcca ttcgaccaag aatatgtgta tggagttgtg cctcctgttg gatatactgt
    23341 tgcagaacca taaccaattc catcgcaaac ggggtgttgg acactagtaa aacttgctaa
    23401 aagttgtgga ggttggttta tagtaacaga agtttcagaa gtacaattgt ttccatcagt
    23461 aacagtaact gtataagttc ctgcacataa atttgaagca gtttcatttt gacttggatt
    23521 tccactccat gcgtaagaat aaggagcaat tccattagct gaaactgtcg ctgttccaag
    23581 acaatttcca aagcaaggat tgtgagttaa gtctggagga gaccaagaaa tattgtcaat
    23641 gtcaacataa acagagtttg ttgtagtaca acccatttga tttgtgattg taacagtgta
    23701 tgtagttggt gtagttggct taactaaagg cgagtttgta tttgctccac taataattgt
    23761 agaacttggt tgccagctat aagttaaaat atcagatggt ttattgtttg ttattgttaa
    23821 gtgagtttca tcgccaggac ataaaatctg atcaggaaca atagaataat tgaattgatg
    23881 aacgttaaca tttacacttt ctattaaaaa agtattgcaa acatcttctt tgactctaac
    23941 gtagtaggtt gtgttttgac ttgggcttac ggttagattt tgtgaatttg ataaaattga
    24001 gttaaaactt gtacttgttg accacgccca gctatctacg tctccagttg atgtggcatg
    24061 taaatgagca gttcctcctg gacaaattgt tgtatctgca gatgcgtata aatcaatatc
    24121 gggttcataa atagaaacag tttgtattaa agaatcaacg caaattcctg ttgctatcaa
    24181 tatatactct gttgtttcag ttggtgttgc tactggattt ttaattgttg tattattcaa
    24241 attagagcct tgaacccatg aaaaagtggt tcctgctgga taatttccct caggaccaat
    24301 aacaatactt tgtccgggac aaatttctaa atcaggcaaa gtagaaagcc caggttctat
    24361 tacagaaaat gttctttgta tagtgtcgta aaagttgcaa cttaatgggt ctccaacaat
    24421 taaagttaca gtatattctc ctccaactgt ataagtgtga tttggattta gagcagtgct
    24481 tgttggcgaa ccgtctccaa aatcccataa aaaactagtt ccttgcgaag tgttttgaaa
    24541 atttacagta taaggagcac agccagcagg aataggatcg aagctagcgt cagctaagtt
    24601 ttctataatt cttattttaa aaactgcgtt attacaattt gaagaattat ttgtagaaga
    24661 ccaaactccg ggattaggtg ccgttggaaa ttgttggcaa gcaccacaac ttgagcatac
    24721 ggattgaata atatgtcctt ttttatcaaa acgacttgtg ccaccgtcaa catggtcgtg
    24781 tccagaatac gaacatccat cataatgcaa ttcgccaaaa aagcttgcat attctaattc
    24841 cgaagcgtct tcgcttaaaa ccataacata aaaatcttgt ccatctgttt cggtttggat
    24901 agcatcagca gttatttcca ttcctttagt tccataatca tcttcccaag caaacgtatc
    24961 ccattgagca ttataaggcg aagaaaccca ttctctgcca cagccagtta aataaatacg
    25021 gttgcaaata tcaaccgcaa aagctgtaat tgaaatattt ggtcgtccat tatctgttcc
    25081 aaagcgagtt gaccaaacca aagaacttaa atttgaatta aatttgctta taaattgtcc
    25141 actatttggg tgattatatg tagcattata aactagagca tttgcaggag cttttgtgag
    25201 cccagtaata aaaatatgtc caaatttatc agaccttaca aaataagcat tgtcaaaagc
    25261 agaagagcca aaatatgatg aagctaagag cgtatttccc atattgttta attttgaaac
    25321 aaaagcatcg gttgaaccgc cattatggga agtgttataa gcaccactag taacagggaa
    25381 attatgtgaa acagtagcac ctgtaactaa aacatcttcg ttaggactta aatccatact
    25441 aaaaatagcg tcatcttcgt ttccacccaa atatgaactc caaatcaatt gacttaaatc
    25501 tggattgagt ttaaaaacaa ttccatcttg ttttccaccg cttgtagtct gatatccagg
    25561 atttattccg ctaggaaagt ctgttgagaa agtatttgtg cctacataga cattattttt
    25621 accatcaaca ataacttcgc ctcttgctcc gtcagcataa ttataataaa gcgagtcgtt
    25681 tccgtgcatt aacacatagt gtattgccat gtgtggttta aaatttaatc catcattacc
    25741 actgccacct acgtaagtgc tagcaagtaa ttgagttcca tcttcgctaa gttttgtaac
    25801 gaaaatatca actccattat taaagccaat aacattatca taaactacac tgtcgcctcc
    25861 tgcgaatgtg gtctgaaaag catttgtact cacaggaaaa tcatcggcac cagtagttcc
    25921 cataattacg agttcatttt cttcagtaac aatcagactg tgaggcattt cttgtccatt
    25981 agacccacca agataagttg cataaaggcg ttgagttcca tttgcattat atttaataat
    26041 tccaacatca caaccatttg cataaaaaga actaccagca attggtgctg taccacctgc
    26101 aaaatttatt tgatatgcac ctgtacttgt tggatagcct atgtcaaaaa caatcccgcc
    26161 agaataaaca ttgtcgttat agtcccaagt tgcggtaaat ccccaattat cacctgtaga
    26221 acctgtatat gttgaaaaaa ctaaagatgg atcaattatt aaatcacgag aattatcaaa
    26281 accattagga aaagagaaac taagtttatt tttctttaat ttgtaattac aacgaatagg
    26341 tattttttcg ccatcgaaaa tttgataagc aaaaggctgt aactcaatga cttcatttac
    26401 acttgttgta actaccaatt gattgtcttt aatcttgata ttatcaacac cttcatattc
    26461 taatattatg tctttgtaat ttccagaagg ctttacgatg aagtcatatt taacgccttt
    26521 ttcatgagta tagtacaata aatcaatttg attataaatg tctgtgtata agactttttc
    26581 gtatagtttt acatttgaag cccagttttt gggattatta ccaataaagt aatttacata
    26641 atctaattgt ggattttgag cttgaatttg cggattttta tttgctccta caaaatgtac
    26701 tttataagcg tgtgcatctt ttaataaatt gttgttgtgt tcttcgcaac catgatgagc
    26761 gtgagaatga gccatactat gtgggtcggt taaagcccaa gttacacaat tttcttcaag
    26821 aaaaagagca gcttcgttta atctaagttt atataaaact ttctcgttcc attgtccttt
    26881 gttttctaca taatttaatt gtgaaaatgc tgagcagaaa acagcaacaa aaaaacaagt
    26941 taaaaatatt ctataaaaaa tcctctttat cataagaaaa acaatatttt ataaaaataa
    27001 tgttttattt tttaatagac aaatattttt tgcaaaacct tataaaatat tttcttaact
    27061 aagtttttta taagtattaa taatctcgtt tttaaagtta aaatctttga tagcaaagaa
    27121 atcaggagtt ttaagacttt ctgaattata aataatttct ttgcctgttt gaaaagagaa
    27181 aatatttcca cctgcagctt ttaatactgc atgtccagcg gcaatatccc attcgttaat
    27241 ttgtcctatt cgaagataaa tgtttgcaga attgcttgca acagcacata actttaatga
    27301 gcttccaaca tttctacatt cgctttctgg aaatttttct ttaaaagtat ttacaaagct
    27361 tgtagtgttt gagttcaagt gtgatttgct tataacaaat tttggaattt cgatcttgtg
    27421 atctaatgta atattttcaa ttttgtttgt taatgtattt atttttacag aattagaatt
    27481 tacatttcca taatataatt ctgatttagc aggagcaaat acaacgcctt ctactacttt
    27541 gttttcacaa actaaagcaa tgtttatagt gtattcgtct tcgcctttta caaattgctt
    27601 agtaccgtct aacgggtcaa ttatccaaaa ttttttaaga tcttttctgt cttggtaact
    27661 taaattttct atctcttcgc taataatggc gatattagtt ttttttaagc cttcaataat
    27721 aattttgttt gctgaaatat ctgctatcgt aacaggcgaa ttgtctgctt ttagatttac
    27781 atcaaaattt tttctaaaat attttaaagt ttcttttcct gctttaattg ctaattcaat
    27841 aagtaaattt ttattttctt gaatttgcat attttacgaa tttcttacaa catttgcaga
    27901 cgaagcctga gcaagtggag ttaccaaaat atcgttaatg tttacgtgtt ttggtcgatt
    27961 agctacaaat aaaataattt cggcaatatc ttcggcgtac aatggtgtga gtccatcata
    28021 tactttatct gctctttcct tatcgccatg gaaacggaca gttgaaaatt ctgtatccac
    28081 cattccagga gctatttgtg taacttttat tttatatgga agcatatcta tacgcatagc
    28141 tttgcttaaa gcatcaacag cgtgttttgt tgcacaatat acatttccgt tgggatatac
    28201 ttctttgcca gcaatagaac caatgttaat aatatgtcca gatttttgct ctatcattaa
    28261 aggagcaact tttaaagtta aatacaaaag tcctttaata ttggtgtcta tcattgtttc
    28321 ccaatcttgt aaatcaccag tttgaatagt gcttaatccc attgctaagc cagcgttatt
    28381 tattagtaaa tcaattttct taaaatcttc gggtaaactg tcaatagctt tgcaaacttc
    28441 atcattagag cgaatatcga aatttaggca aaatactttt gcatttgatt ttgcttcgat
    28501 ttcagatttt aaatcatcta atctttgttt gcgtcttcct gttaaaatta aattgtatcc
    28561 gttttcagac aataatagtg ctgtggcttt tccaattcca gatgtagcac cagatattaa
    28621 agcagttttt ttcattttta taagtttgaa aattatttat ttaaaaatct ttttgcagca
    28681 acatattcgt ggggactgat aaaagttctt gtatcaggca ttacgctata acctttacct
    28741 tcgggattgt atttttcgaa aagtgtaatg attttacctg caaattcttc tacagaatct
    28801 gttctcatgt aattaaattc gggaagattt attgtttggt catttttaaa attatagcag
    28861 gttttacttt gttcggtagt taaagttatt tgcccccaat tgtcaaaaaa tttacttgta
    28921 tttttcaaca tcaaagtaaa atagtcatta tgatttacat tcaaataaat agaattaata
    28981 aagcctatgt tctctttttt gcaaagtgtt gcaaattttt caatccaata ccaatatttt
    29041 ggattatctt gatgaataaa tggaaataaa tcacaactta aattataatc gcaatgtcca
    29101 aatgcaatat ttttaaatct agggtcttta aactttatta tttcttctaa attattaaaa
    29161 gcctcattac tttcgataat tggaataact tcatcgtagc gaatgtcttc tgttgattct
    29221 aaaaatgttt ttaaaacttc aacagacgaa caattagata aaaatatgca acgaattttt
    29281 atgtttccta aacttcttat tgcttcaata tcatcgaaat atcgatttgt gccgtattca
    29341 ttaattctaa atcctaacca agtttttaaa attttaactt taaatttatt taaaaacttc
    29401 aaaacttttt ctctatggtc tgatttatag cgattagaat cattggtttt aaaatctaaa
    29461 acgctatctt cgaaatcaaa acatgaaact gtgtttattt cagttaatga acgatgaaat
    29521 tcaacaaaag agtcttctct aaatttaaca aactgatatg aagtaatcat aagctcaaaa
    29581 tattatttat cccacttagt catgttataa gaactttgat aatataatct gaaaattcgt
    29641 tttaacattt ttattaaaat tctgttacaa aagtacaaaa aaatctaaaa tttagaaagt
    29701 aaacttagaa aaatcttaat tttaagtact ttaaagaagt taagtatttg attgtattgt
    29761 gtttaattta tttgtgaatc aacaaattca gcaagttgtc ctaaacaata aacatagctt
    29821 ccatactcat tatcgtacca gcctaatatt tttgcatgag ttacaggcaa gtttacagca
    29881 ttagcttctt taatccctgc ttgtaacaaa gtttcaggat gtagttgcaa aaatcctgtt
    29941 ctggtgtgca attcatttcc ttcaataatt acagcagcat ttagtcccat taaatctgta
    30001 gaaacatttt gaaggtcaga gaagtataaa agattttttt gagcgccagc agcagcggtt
    30061 ttgtaaatat cttttatgta ttgagcattt atgaatggca ttgacaattc gtttaagcgt
    30121 gaagtaaagg taatgtttag cgaaataagc gaaactgttg gtgtaggaat acgaatagag
    30181 tcagccataa atccaaaact agttgcttgt ggtattactc tttctaaagt ttttgcagca
    30241 cctgtagaac ttaaaataat attgttgagt acagatctgt tttttctcaa atcagaggtg
    30301 cctgtagcag gaacagaatc taaaacactt tgtgtattag tagctgcgtg tacagttgtc
    30361 atactagcag ttaaaactct tttagtttct tcatcttcta aaagtggttt taccatatga
    30421 gccaaacctg ttgttgtaca actagcagca gaaataatat ggtgtttttc agcatcaaaa
    30481 ctattgtggt taattccata aataagagtt ggatactcgt cagggttgtc taatgctatg
    30541 tttttaattt taaatggcgc actggcaata acttttaaag ctccagcttc tatatgacca
    30601 gcaatgcttc ctttagggtc gtttgcaggt acagttgggt ctaaaaactt tccagtacaa
    30661 tcaacaacaa ctttaacccc tgcttctctc cattttatgt ccttaggatt tctatctgta
    30721 tataaaaatc tgacagaaaa accatcaatg cttatcaaac ctgcgtctct atcaacaatt
    30781 ttgatttcaa ctttcttgcc actatgacca tataggaaat tatccaatct accgtaagtg
    30841 gtgtctgagc ttataacctg aattaagtct tcaaactttt ttcccacttc tcttcctaca
    30901 ttaattacaa taccgtcaaa ataccttttt gacaaatgat accataaggt aagtttaccg
    30961 attctaccta aaccgttaat tcctagtgtt tttttgttag tgatttgtaa cataattttt
    31021 tcttttaaag ttaaaataaa ttttgagttt tcaaaggata attgccttta tatacatttc
    31081 ctttaattcc atctatagca attaaatcaa gcgaacgaaa ttcaacatct ctaataactg
    31141 cttttttctc attttcataa acaagcatgt cggcacaatt taccacacaa actttaccta
    31201 aacctccagc tgttacagca gcgtgtgatg ttgcaccgcc tttaccagtt aataaacctt
    31261 cgcattcgaa aattaattca atatcatcag gaactgtgtc gggtctaact aacattgcat
    31321 tgtcttcggg gtatttttct ttaagcaatt ttaaatcttt caaatcaaag acaacccttc
    31381 cattcaacac acgatttcca attccaatgc ctgttccaac taattccatg tttttttcta
    31441 gcgtagcaaa gacttctact ttgtcttgct tgtttattat catgcttctt gtttgaagta
    31501 tataaaggtc ttctggttct gtatgttcaa aagtaaattc aatttcttga tgtgcaaagc
    31561 catgattttc aatcaggtcg taagaaattt cttttagttt attgtaaatt tttggaaatc
    31621 cactttctaa agatattgga ctattatgat agtatctttt tctttggttt tcgcttattg
    31681 gcaatgtgtt gattaatcca gcaacaatat cttcgccttg acttaagaaa gaaaaatcgc
    31741 ctgttaaatt aatgccagga atattatctt gtgtgtcgtg agtgaagagt acaccagagc
    31801 cagattcttt atgaaggtta ccaaaaatca tttgttgaat tatcactgct gttccccatt
    31861 catcggcaat ttgcatatgt tctcgatata cttttgctct tgcagagtcc caactgttaa
    31921 aaacagaaag aacagcttgc ataatttgtt taaatggatc tgactcgaaa gtaatattgt
    31981 tatcttctaa atgtttttta tatgcaaaag caatctctct catgattttt ggattgaaat
    32041 caatttttct ttgaatatca tacttagttt tgtattctaa cattatgtta tcaaagtcat
    32101 tcctatctaa tccatacgcc attccccaag tttgtagcaa tcggcggtag caatcccatg
    32161 atgtccaagg atagttgtct tttttgctta gagcttcggt aatttcgtca ttcatgccca
    32221 catttaaaaa agtattcatt gcaccgggca tgctgattgc agctccagat cttacgctca
    32281 tcaataatgg tttttcggga ttaccatact ccaagcctgt aagtcgctct aattccgaaa
    32341 tgtggtatct aatcaaattt tcaatttcgt cgtataatga ttggattttc attattgagt
    32401 ttagtcgcct aaatacttcg gtagtaataa caaaacctgg tggaacagga aaatcgttga
    32461 gatataattt ttttaaataa taagctttag aaccaagaaa aacttgattg tctatctcaa
    32521 gtgtttcgcg ataaattgga ctaataacac tatcaggatt atacgacatt atattttgga
    32581 cctctacatc agataaagta tcagccattt ttcttaattt attaagtact cgcccaataa
    32641 acgcatctaa agactgaaca agaaatgctg aagaaagtaa gtctctataa aatacttctg
    32701 aatgttgtat gattaatttg cgtactgtat gtttgttttt tctgtcttct ttatcaagat
    32761 atgatggtat aataactggt aattgatttt cgtatggacg tataaaataa ttgttgatta
    32821 tttcttttag gtttttttcc atgaattgga aaatgttgat gtattggcga atagtaaaac
    32881 tttcggaact taaactatac tgaagcattt ttaagttaga atctaatcct tggtcataaa
    32941 ttccatcaag ggttaaacct tctctaataa gttgtagtac taaaaaaaca tcttttagtg
    33001 ttcgtgcagt aaagtagtcg gtttttattt cgtctaaaat tctatccact aacgaagagg
    33061 caatttgctc taagcggaaa gtaagtccca ttgcttcaaa tttctcttcg tgatattgtc
    33121 catacataga cggaatacca aaagctatat gacgtttgta ataaatgttt tcccaagctt
    33181 cactttgttt ttcatcaaga attctatcat tcaaaatttt cataatagaa tagatagttt
    33241 tcaaagcttc aaaatgattt ccagcttcaa tatattcgag caaagaattg atagtgtctt
    33301 gttctatata atgatatttg cttaataaag cacctaagtt gttggtggaa aataaatatt
    33361 tttcccttaa taaatgatat aattctatca ttaaagctac acgctgacaa tctttgcagt
    33421 ctgggtcagg aaaactattc aaatgagctt gtaaactatt tttatcaaga ttaattagtt
    33481 cttctaggct gctgttgttt aatttgcaaa gttttactaa aaccttatga actccatcaa
    33541 cccattgccc atcaggatct attgttacaa aaacattctc aggaacaatt gattctaata
    33601 aactcttatc tttagtgtac caaaatttaa taatttctaa agtaatatca atatgagagt
    33661 tgctgccctc ggtatgaatt tgctttctta aaaaatgaat tagcttgtcg ttacgatttg
    33721 ttagttcatc aacaactgtt gtaacatctc taagttgacc ttcggcacca atttcattaa
    33781 aataaacagg gtatattcta agtaattgtt tgacttgctt atagataggc gaaatgttgg
    33841 agtttaaaaa tttactaata tctttttgga aaaggtcggt gtcgaaaatg aaaatacctc
    33901 ctatacgtaa attaattata agcgatgaaa gtagtttttt catcgtaaat gggtcgtaag
    33961 caataagttc ttgccaaacg cgtatgtttt ttatgtggtt tggattaact ttaagttccc
    34021 aatcgtttgt taaataagca acaccaggat ttataaagcc aaataaaatg gttttttctt
    34081 caaaataatg tattaattct ttgttttttg tatttatgat ttctttaccc aaagttaaaa
    34141 tggaatctaa gataaaacta gtattgctat atttaaaatc agcaaataaa gaatataaaa
    34201 tatctatgga attaatacat tgagtagtgc tacattcgct actaattctt ttaatggctt
    34261 tgtttaaatc tactaaaata taatctctgt ggaatattgt tccaggtaga tgaagtaaaa
    34321 agaaaatata aacaaattgg tctgtagctt cttcaaaatc aactattcta tttcgtaaag
    34381 attctataat ttcagaaaaa gtaaagctgt attttatcag ttcattataa tcattggctt
    34441 gatttagttt ttctttataa taattataaa attttttacc aatctcataa atagttgatg
    34501 aatagtcttt tgagaacttg ttttttacac tttcatacca ttcttcaatt ttagttgtgc
    34561 tttcccaaaa ttcaacattg ttgagtaaaa gttgtttagt gaaattaaaa actagctctt
    34621 tactactttc gttatctaca gcttttttta aacaggttct aaaaaatgaa atattactta
    34681 ggtaagcaaa taagttgctt tccatttctt cgtcaagaat ttttataaaa tgttcaattc
    34741 cattttttaa tgattttata tcatcaaaat tatttgagaa aaattgcaga taattttgaa
    34801 caatagattt agtgtattta tcacttatgt ttttttctaa taaatctcta aaaatatcaa
    34861 acagaacatc ataaactttt tctttttcgt ctaactcttt ataaacccaa aactcatcgt
    34921 ttaaaatttt aacaagtccg tcaacaatga ttttgctatt aaaatatggg tgattaagtt
    34981 cttttaaaaa atctgaagtg cgctgattga tgccgtaata atttttagaa agcgacaaaa
    35041 accaaagttg tttttcggga atttcaactt taatcttttg tgtttccgaa agatttacct
    35101 caagagcttt tgaattaatt tcctgtttca taaatttgaa atatatttta ctaagtccac
    35161 aatacgagca gaatacccag tttcgttatc gtaccaagca acaatttgaa taaaattatc
    35221 tcctataact cgtgttagtt gagaatcaaa aattgctgag tgtttgtttc cttgaatatc
    35281 gctactaaca atagggtctt ctgtatattc taaatatttt tttaaatctc cttgtgattc
    35341 tttcaaaaaa gctgaattaa tagcttctac gtcaactttt ttatttaatt gtgcagtaag
    35401 ttccacaaaa gaacaatctg ctaccggtac tctagttgca aaaccatcaa aacgatttgc
    35461 taattctgga tataaaaggg tagtagcttt aattgccgag ctagttgtag gaataatatt
    35521 attcattgcc gaccttgctc tacgcatgtc tttgtgaaat ccatcttgaa gattttggtt
    35581 atttgtaaaa ggatgtacag tattcataaa ggcacttttt acgccaaaat gtctgaataa
    35641 aacttcaaac accattgcaa cgcagtttgt agtacacgat aagttagaaa ttattttatc
    35701 atcattgctt atgttttgct ggtttatgcc cattacaaca gttcgtttga tagacgaatc
    35761 ttcacctggc gagcttaaaa ttactttttc agctcccatt tgcaaatgcc cttgaagact
    35821 tgttgctgtt ttaaatttac cactcgaatc cacaacaata ttaactttat tatctttcca
    35881 tggaatttga gaaggactag taaaatttgt aacaactatt ttctttccgt taacagtgat
    35941 aaaatcatca ccatgcccaa tttctgcatc aaactgccca tgtgaactat cgtattttaa
    36001 aagatgagca atcattttag ctgacatagg gtcgtttatt tgaactaaat caaaactatt
    36061 gtcgcttaat agcattcggc aaactatttt tcctatccta ccgaaaccat ttactcctaa
    36121 tcgtattact tcctttttca taaaaatttt ttaaacgact cagaccaata atgtgtattt
    36181 catataaaat tctttataat gaaatttttt gcaaatataa taataaataa gtttataaag
    36241 tcggtaaagt ttgtaaagtt tttaaagtcg gtcaaactcg caatgctttt cacttttagc
    36301 agaattaaat aattcttcat tcttcattct taattcttaa ttctcaatta ttcaattatc
    36361 aattctcaat tctttaaaaa aacttttcct ataaaattta aaataatgtt aaacattagg
    36421 ctttcattta atgtttttat atttttgtat gtttaaaatt aatgttatga agtttagaat
    36481 atttggttta gtcttgagtg caattttgtt tttcaatttt gctacttatt ctcaagatgt
    36541 aaattcagaa acaagaaaat tcaacaaaat gcttacgctt atcgacgcta tgtatgttga
    36601 tactgttgat atgaaaaaga tagttgagac ggcaattgtt ggaatgttaa aagaaatgga
    36661 cccacactct gtttatatta gtgctgacga aattgaaaaa atgaaagaac ctttagaagg
    36721 aagttttgaa ggaattggtg tacaatttag attaatggaa gatacacttc ttgttgttgg
    36781 agtaatttca ggcggacctt ccgaaaaagt aggaatttta accggcgata gaattgttgt
    36841 agttgatgat gaggttattg caggtgttgg cattgataat gccggagtag caaaacgttt
    36901 gcgtggcaaa aaaggaactg ttgttaaagt tgaagttgta cgtagaggcg aaaaacaaaa
    36961 tttagttttt aatattactc gtgataaaat tccaatttat agcgttgatg cagcttatgt
    37021 aacaaaagat aaaataggat atgtgaaact aaataaattc gcaaaatcaa caaccgaaga
    37081 gcttgattct gtgtttgtta catttgcaaa agcaggcgtt aaagatataa ttttagattt
    37141 aagcggaaat accggcggat acctcgacaa agccgttaac ttatgtgatg aatttttaca
    37201 aagtgataaa ttaattgttt atacagaagg cgctaaaatt cctcgaatag aatataaatc
    37261 aacaaaaaaa ggaagatttt acagaggcaa attagttgta attgttgatg aggcttctgc
    37321 ttcggcaagc gaaattgttt ctggtgcttt gcaagattgg gacagagctg tagtggttgg
    37381 tcgaagaact ttcggaaaag gtttagtaca acgcgaaatg atgcttactg atggctcagc
    37441 agtgagatta acaatagcaa gatattacac accaacaggc agacttattc aaaaaccata
    37501 cgacgaaggc tatgacgaat attctgctga tattgtaaac cgctataaac atggcgaatt
    37561 tttgcacaaa gacagtatag tttttgcaga ttctttgaag tataaaactt tagttaataa
    37621 aaaaactgtt tatggcggtg gcggaattat gccagatgat tttattcctg ttgatacaac
    37681 tttgcgttct gattatcata tagaactttt aagaaaagga gttttattta gtttcgaagc
    37741 aaaatatgtt gacactcaca gagctgaatt aaatgaaaaa tatccagatg ttgaaacttt
    37801 tataaagaaa tttgaagtta cagaccaaat acttgaagag cttttagctc aagctaaaga
    37861 agcaaaaata aatgttgatt ccttgcctgc tgcaacaaaa gagcaagaca aatatttaag
    37921 aacacattta aaatctttaa tagctggcga tatttggaaa aataatgagt tctggatgat
    37981 ctttaacgaa caaaatccat tctttttaag agctcaagaa attattttag acaataaact
    38041 ttacgactcg cttcttaaag gagaataatg acacaaatca ttaattggct tataaccaac
    38101 tgggtagaaa ccatagccgc atttttagga ctgcttggaa tattcctaca aataaaacaa
    38161 aatgcttggt attggtttac agctataata atggttttgc tttatattta tgttttttat
    38221 gtttcgggat tttatgctga tatggctttc caattttatt atttagttgt tggtatttac
    38281 ggctggatac attgggtaag aacaaaaaat atagcaaaaa acgaaaaatt acaagttttt
    38341 aaattatcta aattacataa tatatttgcc ttgcttgctt cggcagtgtt ttttggaata
    38401 atttatttca ttttagtaaa ttttacagat tcgcaagttc ctttgggcga tgcatttaca
    38461 actgctttga gcattactgc aacttggttg cttgctcgca aaattttaga aaattggctt
    38521 tattggattg tagttgattt agtttctaca attttatatg tttacaaatc gctttatcct
    38581 tcagcaatat tgttttctgt gttgactatc ttagctgtag ttggatattt taaatggaga
    38641 aaagctcttg tttatgaata aaactttaaa aatagtatta acaggaccag aatcaaccgg
    38701 caaaacaacc ttagcagagc agttgtcaag tcattattct ttaccatggg tcgaagaact
    38761 tgcacgcgcg tatgtcgaaa gtttaaatcg tccttacaac caagcagatg ttttagaaat
    38821 tgcaaaattg caaatcactc aagaacacaa agccttagct aaaaatcata aagcagtttt
    38881 ttttgatact gatttaatta ttacaaaaat ttggctttta catgtttacg gcacttctcc
    38941 aagctggata gaccaagaaa tttctaatta taaaaactgc atacatttat tgtgttaccc
    39001 cgatttagaa tgggttgctg atggcgttag agaaaatccc gacataagat tatatttatt
    39061 tgaaaaatac gaattagaaa taaaaaacta tggctttcac tacaaaatca tcaaaggaag
    39121 cgaagacgga agactcaaac gagccttaga gtttgtaaac ttgtatttaa gataagaatc
    39181 ctggtgttgt gattacaagc agagtgagct tgcgaactct gcgaagcaac agacgcgtca
    39241 gcctctttta gcttttaact taagtaattc ttcattctta attctcaatt atcaattatc
    39301 aattattttt caacttttaa ctgtggtttt tttctacaaa ttccaaaaca gaatcgcaaa
    39361 tatattttaa tgttttatca tctaattctg tattcatagg aagtgaaata acgctttcgc
    39421 atagcttgtc ggtaacagga aaatcttttg aattatattt aaaatcttga aaagcttttt
    39481 gattatgaag agcaacagga taataaacag ccgatgcaat tccttttgaa cttagatgtt
    39541 ccatcaaagc ttttctatca acattattta aaaccaaagt gtattggtgg aaagtgtgtg
    39601 tagaaaattt tgaacgagtt ggagttttaa tttttgagca attttcaaaa gctttgtcat
    39661 aataatctgc tgctttgcga cgagctgtat tataattatc aagatgagga agtttcgttc
    39721 taagaaccgc tgcttgaatt gtgtctaagc gtgagtttac accaattcta tcgtggtaat
    39781 atctcacttc cattccatga tttgcaatag aagttatttt ttcggctaac tcatcattat
    39841 ttgtaaaaat agcaccgcca tcgccatagc aacccaaatt ttttgaaggg aaaaatgaag
    39901 tgcaaccaat atcgccaata gtgccaactt tttttgtcca gccttttgca gaagtaaaat
    39961 cacttcctat agcttgtgca gtatcttcga ttacaaataa attatgtttt ttcgcaatat
    40021 ccataattct gtccatatcg caggattgtc caaaaagatg aacaggaata attgccttag
    40081 tctttggagt aattgctttt tcaattctgt cagggtctat gcagaaagta ttagggtcaa
    40141 catcaaccat tacaggtgtg agttttagta aagcaatagt ttctactgtt gcgataaaag
    40201 taaagtcaga agaaataact tcatcgccag gcactaaacc aagactcatc attgcaattt
    40261 gcaaggcatc tgtgccgttg ccacaaggaa taacatgctt tacattcaaa tattcagcaa
    40321 gttctttttt aaattccttt acatctgtac catttacaaa ttcagtattt tcaataactt
    40381 tagcaattcg agcatcaact tcatctttta tttttagata ttgtgatttt aaatcaacca
    40441 ttggaatatt catagcaaaa aatttttata aattaataat ttagacaact ttaaaacatg
    40501 caaactttct taatatctcc tcaaatcatt cttaatcttt tcgctaagtt tttggcttgc
    40561 aggaaccata ggtagtctaa attcattttt cattttgcct tgttgagaca aatattcttt
    40621 tacgccacaa ggatttcctt ccgcaaacat atttgaaata gaatcaagaa gtttataatg
    40681 aatagtttta gcgttttcaa aatctttgtt taaagctaat cttactaatt gtgacatttc
    40741 ttttggaaat gcatttcctg caactgaaat aacaccttcg ccaccaactg caattaaagg
    40801 aagtgttaag ccatcatcgc ccgaaataac tgtaaaactt cgaggtttgt ttttaataac
    40861 ttgcatcatt tggtcgattg aaccgcaagc ttcttttatt gctgttatgt tttcaaaatc
    40921 gtttgccaat cttacaactg tatctggaag aatatttact cctgttctgc cgggaacatt
    40981 atataaaatt aattgtgtag gacaaacact tgctatagtc gcaaaatgtt gatataaacc
    41041 ttcttgattt ggcttgttgt aataaggagc aacacataaa atgccatcaa ctccattaag
    41101 atttgttgat tttatacgat taattacgtc cattgtgttg ttgccaccaa ttccatacat
    41161 aattggaact ctagagttta tttctttttt tgcaaactct aaaatttctt gcttttcttc
    41221 ttcagaaatt gttgccgatt cagctgtagt acccattaca agcaagtaat caactccatt
    41281 agcaattaaa tcattaatta attttgcaaa agcaggaaaa tctatttgtt tgtcttctgt
    41341 aaacggcgta accattgcta caccagttcc tataaaatta gttttcataa tattttaagt
    41401 atttatcatt tttaaataat gttcagtttg ttttattaat tcttcaagca agttttcagt
    41461 gtttttatca atatttatca tcaaatcgta atacacaggc tcgtttttac tataaagtcc
    41521 agttttaaac attgcttgag attctgctac aataaagcag tttggtaatt tttcattaat
    41581 gtttaaatca attaaaatat cgaatttaca attgcaaaat tccttagtaa tttcagaaat
    41641 tggtttaaaa aacttattca aatctttttt agaataaaac ttaaaatctt catgttttgt
    41701 gtgaaattct gataattcag cagagtcaac atatccaatg ctgtcaactt tatgaatttt
    41761 gctttttaat tcttcaacaa aagttttaac tttttcaaaa tcttcgttta atgtggcatc
    41821 ataaactata cctattgatt ttgctttata aaggttgcaa gcaatggcat ttgcttcggg
    41881 tcgtcgggca gtttttctaa attttcttaa cccaatttta tatcttaatc tttcgataaa
    41941 cttcacagtt tttttctgca aatttaatta aatataagaa tattgaaaag ttttttataa
    42001 ttttgccttt agaagtaggc aggtctgtcg cctcgttagt tttagcgggg aggaaagtcc
    42061 ggacaacaca gggtgctgca ctcttgaaat aagagatgtt cgtgaggcat gaaggcaaag
    42121 aagaaaataa ccgcccaagt ttatcttgtg gtaaggatga gaaggtagag taagagccta
    42181 ccagtgataa tggcaacatt atcagctgtt tgccctgcag gttgcaagac catgtatatc
    42241 ggcgttttaa agctacccgc ttgagccgag gggtaggtcg catagataaa tgacagacgg
    42301 catactatat gttacagaat ccggcttata tgcctacttc tattttttaa agaatgaaat
    42361 tatgacaaac gaatggaaaa aacgcgaagg tattgtttac tcaactaaag ccgactttgt
    42421 ttatcaaaca aacgagatag aagagccaga aaccttaccc gcagaaaagc aaaaattgaa
    42481 aatagcttta gatagaaagc aaagaaaagg taaaactgta gttctaataa caggatttgt
    42541 tggcaaatca gatgatttag aaaatttagc caaagaattg aaaaataaat gtggaactgg
    42601 aggttcagca aaaaatggag aaattattat tcaaggcgaa atgattgaaa aagtaaaaga
    42661 tattttaaga aataaaggat ttaaagttta aaagagatat gaaaatcaaa aatttaattt
    42721 tagttagcac attaatatta ataagtgcat ggtcttgtgg agataaaaaa tctaaaacag
    42781 aaaaacaaga cgaacatttg attgaaatag ctttaaatat tgctaattat tcaaacgatt
    42841 atatcgtagc aacttgtaac gagcaggttg atactgttaa agttggtgaa gatggaaaat
    42901 atttgtactc aacttttctt ggtcaagatt cttatttaga aattaaaata ggaagaaaaa
    42961 gagttttgat atatactaag ccaaaagccg aaatgcaaat agaagttgat gttcaagatt
    43021 ttcataattc tttgaaattt tctggcgatt atgccgaaat aaataattac ttagcaaaac
    43081 aatctaaatt attatttgac gctaaaccag cttccgaaaa ttttatttat gcaggagatt
    43141 attccacttt cagcaattca tttaaaaaat atgatgacga aagtaaaaat aacttgaata
    43201 agttcgtgga agcaaatcca aatttcgagg actttgtgat tttagaaaaa gaaagacaaa
    43261 aattaattaa tacaattttg tatttgtctt attattctcc tttggtttat accgaacaaa
    43321 gcgatgaggc acttgaaaat gaaataaact ctttaattaa ttccacagac aaaaataatc
    43381 ctaagttatt gtgtttgaga atattttatg aatatgcgga aactttaatt ccttataaat
    43441 taaatttaaa ataccgtgaa gctggaatcg aaaatattga tgatgctgaa tatattaatg
    43501 attattttga tttaattggt gaattgtttt cagaatcaga ggtttatgaa gctgtttttt
    43561 atagtggact aaaatctttt attatgtatt atgggacaga aagtgtgagt gaacaatttt
    43621 caaaatatga agattttagt atgaatagag agcatttgtc agagttgaat aaaatttttg
    43681 ctgaatatga taaattaaaa ccaggtaaac ctagcattga ctgggaattt cctgataaag
    43741 atgggaaaat ttatagctca aaagattttg caggaaaata tatttatatt gacgtgtggg
    43801 ctagctggtg tggaccatgt gttagagaat caaaatttta taaagaaatt gcaactaagt
    43861 ttaaaggaaa aaatattgca tttgtatcca tttcagttga tgaaaataaa gacgagtgga
    43921 caaattttat tggtaacgaa aacaataata ttgtaaatct ttgggcagga ggttggaata
    43981 acgacctgtg caacttcttt aaaatcaatt caataccacg ctttatttta attgataaaa
    44041 atgggttaat tataaatgca aatgccagca atccatctgg tgatatagaa gaagttttga
    44101 ataatttaga tttgtaaaaa attttatttt acttttccgg tggtaaataa aaatattcta
    44161 cttcttcatc gtcaagttct ggactagggt aaacacttat tgttttggtt tttaaaatgg
    44221 tattgtcttt taaactgtaa ttattaggtt caaaaaccaa tctgtacaag ttgtataaaa
    44281 acatataatc catgcctaca tattcataat ggcgtgtcga acttttattg ccctcattat
    44341 tgggccaaac taatcttgac gaagccgacc aataagggtg gtagtcattt cgggaggtat
    44401 cactcgaagg accagaaaat ggagcaaaac ttaatagttg ttggtaaaaa atcttatcgt
    44461 tttcataaag ctggttgccg ggtttgtaaa tgttttggta tttttgatta tgtaaagtta
    44521 ctagaattaa agggcagtgt tcataagtgt atttgttgcg tttgtcgctt aataaaaata
    44581 ataaatcgta agattttgta ttccaattta tatctgaaat actagctaaa tctctaaaca
    44641 tataatcgtc aaaattttca ggcataggaa atcctgcaag ccacaagatt tttaaatttt
    44701 taaataatat agatttaaac acataagcag ccaagccttt atgagaattg tcgtagtgat
    44761 aaatatcatt tcctgttata ctttttcctg cttctccaaa gccgtatgaa ttaatccaaa
    44821 ctcccatatc ttcgccattt ccttcagcaa ccaagtcgtt tgtaacaggg ttcattatat
    44881 accaataact agaaaataat gcaccaaatc ttgtttgaga aggacgtgct ttaaactcat
    44941 tttttggctt taaaacaatt ctttgttgtg gatattcttg atgacattgt ctaactaatc
    45001 tgtctgttag gtcatcaacc cattttttaa aagaaatagt gtttccttgc catatgcctt
    45061 tagattttaa ataatttgga ataaaatcat ttctgaattt tacttttatt ttgctaacat
    45121 cttcatcgtc aaccaatttt ttaactaaag ctaagccttg caacatcctt acaatattat
    45181 cttgactcat tccttgctgt ggaattacgc ctttttgaaa aatagacaaa tatctgttag
    45241 tgccgttttt aattttatta tacttgccat attcttcgtt aaaatgtttg taatactgtt
    45301 tccaaaagcc taagctaaca tcgtctctaa ttaaaaatcc atttatatcc tctggatatt
    45361 ttataagttc aaaatcgctt ttgttgggtt ctgttttatg atattttcgc aaagtgtatt
    45421 ccgagtaaag gtctagtctt tctaaggcaa tcatagcata gagcaacata gataaagttt
    45481 cttgataatc ttgattattg ttttttaata atctgtattc tgtagaaaga agtcctaacc
    45541 aatgattaaa attactgttg ccatcactcc aactaaccca agatatattt ccagctttat
    45601 ctaatcgtct atctacagca ggaatgttaa cgccaaactg ctcaacatca gaacttataa
    45661 taacccattc gttaagtaat ctgtttctat aatttatata ttttgaatta agggaatctg
    45721 taatgtttgc aaatgtgtaa cttgtagcaa aaatcactaa aaatagtatg tttattattt
    45781 taaatttcat tttaaaattt tttcaaaaat aattagtttt ttcaatttaa cataaacttg
    45841 ttaatactta taatgaaaaa tagggtgtta atattttgag aataggtatt tttgaataaa
    45901 aaataaatgc gtattttatt tacaatatat tgctttttta ttgcaggttt agcatattca
    45961 caaatcacgc cagagcaata tgtttctaca tatagcgatt tggctgtgaa agaaatgaaa
    46021 agaacaggag ttccggcaag tataacttta gcacaaggaa tgttggagtc gggaaatgga
    46081 aattcgtatt tggcaacaaa agggaaaaat cactttggaa taaaatgcca taagtggact
    46141 ggagacaaaa tttatagaga tgatgatgca aaagatgaat gttttagaaa atataaaaat
    46201 gtagagcagt cttttatcga ccactctaac tttttgaaag aaaattctag ataccatttt
    46261 ttatttgatt tagaaattac agattataaa gcttgggcta aaggacttaa aaaagcaggt
    46321 tatgcaacaa atcccaatta tgcaacatct ttaataaatt taatcgaaaa gcatgagtta
    46381 tataactttg atttaggaat ttctaaacca aaagaagata agccaacaaa acgtccagaa
    46441 agaacaaaag ctcctgtgaa agatgatttt attgtaaatc catatctcaa tgcatataat
    46501 tacaataaca acgtgccata tattgttgtt gttgatacca tagaaattgc agatttagca
    46561 cgccaattgg atatgatgag gtggcaattg gtaaaatata acgatttaga taaaaattca
    46621 atattatatc caggtgataa tatttacatt aaaccaaaac gcaataaagc tgaaaaacaa
    46681 tacaatgttc atattgttga gcaaaatgaa actgtaagag atatatctga aaaatatgca
    46741 gtaaaatcaa aaaaattgtt gaaatttaac gacttaacag atattgacca agaattaaaa
    46801 gtaggtagcg aaataaaatt aagaagataa tttatagtag tgataaatta attaaaaaac
    46861 caaatctaaa ctacagtttt tataaacaag ttttgttgaa aaatagaatt tgttaattat
    46921 ggaaaatcca aagattaatt atatctttaa acacttttgt ttaaaagttc aatttattga
    46981 ttataaagaa attacgaaat taaaaatatt taataattat gttggaagaa gaaaaaagct
    47041 cgagagtaat taggattaat atagaagatg aaatgaaatc tgcttacatt gattattcaa
    47101 tgtcggttat cgtttctcgt gctttacctg atgttagaga tggcttaaaa ccagttcaaa
    47161 gaagaatttt gtacggaatg tcggaacttg gtttggcttc aaataaacct tataaaaaat
    47221 ctgctagaat tgtgggtgaa gttttaggaa aataccaccc tcatggagac tcatctgttt
    47281 atttagcaat ggttcgtatg gctcaagatt gggctttgcg ttatacatta gttgatggac
    47341 aaggaaactt cggctctgtt gacggtgata gcccagcagc tatgcgttat accgaagcaa
    47401 gaatggctaa aatcgctgac gaaattttga tggatataga aaaagaaaca gtagatttta
    47461 atttaaactt cgacgattct ctagaagaac caagcgttat gcctacacga ctaccaaact
    47521 tgttggtgaa tggtgctagc ggtattgctg taggtatggc aacaaatatg gctcctcata
    47581 atcttagcca aactgttgat tgtatcgttg cttatatcga caataacgaa attactagcg
    47641 gagaattagg cgaaattatg caagcacctg atttccctac tggaggtata atttatggag
    47701 ttgaaggtgt acgcgaagct ttcgaaacag gaagaggaag agtagtaatt agagcaaaag
    47761 cagaaatcga agtcggtgat aacggaagag aaaaaataat tgttaccgaa attccttaca
    47821 tggtaaataa atcagaactt ataattaaaa tagctgattt aattaacgat aaaaaaatag
    47881 acggaatttc aaatatcaac gacgaatcag accgcgaagg aatgagaata gtgattgata
    47941 ttaaacgcga tgcagttgcc aacattgttc ttaataaact atataagtta acagcattac
    48001 aatcttcgtt tagcataaat aatatcgctc tagtaaaagg tcgtcctatg acactaaacc
    48061 tttatgattt agtaaaatat tttgttgaac atcgccacga tgtagtagtg cgtagaacag
    48121 aatttgacct taaaaaagcc gaagaacgcg cccatatcct cgaaggttta attatagcta
    48181 gcgataatat agatgaagtt attcgcataa ttcgtgcttc acaatcgccc gaccaagcaa
    48241 gagaggcttt aatggaaagg tttaaattga ccgaaattca agcaagagct attgtcgaaa
    48301 tgcgtttgag gcaacttacc ggactcgaac aagacaaact tagagctgaa tatgcagaac
    48361 taatggaaaa aatcgcatac tataaagaaa ttttaagcaa tttaagtttg agaatgcaaa
    48421 ttattaaaga cgaaatgctt gaaattaaag aaaagtatgg tgatgaaaga aaaactgaaa
    48481 ttgtatatgc ttcagaagaa ttaaatccag aagattttta tgcagacgaa gatattgtta
    48541 ttacaatttc tgacatggga tatattaaac gtactgcttt aacagagttc cgtgcacaaa
    48601 atagaggagg agtaggagca aaaggttcta caactcgtga agaagacttt atagaacatg
    48661 tatttgtggc atcaatgcat gatgttctat tgttgtttac taaaaaagga aaatgttatt
    48721 ggataagagc attcgaagtg ccagaaggaa caaaaacatc aaaaggtaga gcaattcaaa
    48781 atttaataaa tatagaacct gatgatgaaa taaaagcatt tatgaacgtg gtagcaaaaa
    48841 gagacgatga ctacttagat aattcatata ttataatgtg tactaaaaat ggtgttttca
    48901 aaaaaaccag actaagagaa tattctagac cacgtcaaac tggagttaat gcaataaata
    48961 ttcgcgaagg cgatagctta ttgaccgcta atcttattga agataaacca ggcgaagaac
    49021 cagtaaatat catgataggt actaaaaatg gaaaagtagt tcgcttccct cacgatttag
    49081 ttagaccaat tggtagaact gctacaggag taaaaggtat tactctcgat gaaggttcag
    49141 aagtaattgg aatgattaca gttagaacag accaagatgt tttggtagtg tctgaaaatg
    49201 gatatggaaa acgttctagc atagatgatt atagagttac taatagaggc ggaaaaggcg
    49261 ttaaaacaat taatattact aaaaaaacgg gagatttaat agatatttcc gcagttacag
    49321 ataacgacca cttaatcatt acaaataaat caggaattac aatacgactt gcagttgaca
    49381 cattaagagt tatgggaaga gcaactcaag gggtaagact aataaatctc agagacgacg
    49441 actctatagc ttctgttgca aaaattgaag atggagcatt agaagaaaaa aatgctgaaa
    49501 atcatgaaga actagatgaa aataatggag aaaatctaga aaataatgat gaaaatgcaa
    49561 ataatgatag caaagagtaa atttttggca aacaaattgt atatttgtat tttaaagaat
    49621 atgaattatt aaatcgaaat attatgagaa agttaatgtt tttaatcgca acaatcgctc
    49681 taagtttttc gctttcagca caaaaatcag tagtaacaag tgcatggagc tatctaaaag
    49741 atg
//
LOCUS       DAOPNX010000007        48781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_932_length_48781_cov_6.202294, whole genome shotgun sequence.
ACCESSION   DAOPNX010000007 DAOPNX010000000
VERSION     DAOPNX010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 48781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48781
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_932_length_48781_cov_6.202294"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1061)
                     /locus_tag="PKN53_01420"
     CDS             complement(117..1061)
                     /locus_tag="PKN53_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87646.1"
                     /translation="MKKQKKRMATSYEQLNILMFSNFDLGGDFSCDETDEETPAESKE
                     VLTDVPEEEVVKVDPFKDFPKGNIIAQEGDYVLTPSLNWQIDATKKGPESQTFIFYSA
                     KMAKPGNEYSVIDFIFDDDTEIPNYMIIPIRPDESAKVGDIVLTWWQSGSGMKRAIVT
                     NASDPKAPEVNYIDIDWNNPAKTDDVGIGQKTEKIEPNTFHVLKNKWEAGTSVAVKVN
                     NDYKVATIVNVSGDKVLTIGWAGIMKIYDKNDCVALDIIPNVKVGDEIQAPWVGKFVN
                     TKVVKVDKKFGRVWCDDPHSDDPMIVPFGNIATTLEIK"
     gene            complement(1024..1371)
                     /locus_tag="PKN53_01425"
     CDS             complement(1024..1371)
                     /locus_tag="PKN53_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87647.1"
                     /translation="MKKVLLFTVSALIIFGLSSCGSKKAENLADLKQKYDGKEFADCD
                     EYIKAANEVIDVFIATVEKATKGDEKAFKELEELGAIMVQFGKQEKKLEKECPDKIKA
                     FNEKAKKKNGNFL"
     gene            complement(1582..2055)
                     /locus_tag="PKN53_01430"
     CDS             complement(1582..2055)
                     /locus_tag="PKN53_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87648.1"
                     /translation="MKKVLLFAISALIIFGLTSCNKKAENLADLKKKYDGKEFADCDK
                     YIEAAKEIIDIYIATVEKAAKGDENAKKEFEEFDAFMEQFYKQQEKFENECPEKLEAL
                     EEEISKKMEALLPQLMTLMFGEMDLGDDFSWDETEDVDVESEEVLTDVPEEEVVE"
     gene            complement(2182..2661)
                     /locus_tag="PKN53_01435"
     CDS             complement(2182..2661)
                     /locus_tag="PKN53_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87649.1"
                     /translation="MKKVLLFAISAVIILGLSSCGKKKAENFADIKAKYEGKTFANCD
                     EYIEASTEIIDVYIATVKKAAEGDEAAKKEFEEFTTFMENFDGDEAKFQAECPEKLEA
                     FEKEVEKKMEAVFPQLMTLMFGEMDLGDDFSWDEEEVAVDEEMTEEVAEEVAEEVVE"
     gene            complement(2831..3310)
                     /gene="coaD"
                     /locus_tag="PKN53_01440"
     CDS             complement(2831..3310)
                     /gene="coaD"
                     /locus_tag="PKN53_01440"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOD87650.1"
                     /translation="MDKLSNCAIFPGSFDPFTAGHESVVRRALPLFDKIIIGIGDNNS
                     KKEFLSLEKRLAIIKKVFADEPKIDVKIYSGLTVDFCKNNNCKFILRGLRTSADFEFE
                     RAIGQTNKLLENKIETVFILTLPEHTFISSSIVRDIIKNKGDLSKFLPKGIDETDLK"
     gene            complement(3303..4979)
                     /locus_tag="PKN53_01445"
     CDS             complement(3303..4979)
                     /locus_tag="PKN53_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOD87651.1"
                     /translation="MEEKKEALNFIEQIITNDLASGKHKSIITRFPPEPNGYLHIGHA
                     KSICLNFGLKETFNGKTNLRFDDTNPSKEELEYVNSIIEDVHWLGFEYDNLFYASDYF
                     DQLYEWAIMLIKDGKAYVDEQTPQDISLQRTNPTTPGIESPYRNRSVEENLRLFEEMR
                     AGKHDEGSMVLRAKIDMNSPNMHMRDPVMYRIMKQSHDRTGDKWCIYPTYDYAHGQSD
                     YIEGITHSICTLEFEVHRPLYDWFLDQIDTSGKRPRQIEFARLNLSYTVMSKRKLLEL
                     VKGNYVKGWDDPRMPTISGLRRRGYTPESIRNFADRIGVAKRENIIDVGLLEFFIRED
                     LNKKANRVMAVLDPIKVVITNYPEGKTEMLEAQNNPEDETAGTRNLSFSREIFIERDD
                     FMEDAPSKFFRLKPGGEVRLRYAYFIKCESVVKDKDGNITEIHCTYDPESKGGKSPDG
                     RKVKGTIHWVDSKTCIDAELRLYDRLFTKENPDDLEEGETYLDYLNPDSLQIISAKLE
                     KHLENAEIGNKFQFERKGYFCVDKDSSHKKLVFNRIVDLKDSWSKISKNG"
     gene            5088..5462
                     /gene="folB"
                     /locus_tag="PKN53_01450"
     CDS             5088..5462
                     /gene="folB"
                     /locus_tag="PKN53_01450"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOD87652.1"
                     /translation="MNKILITGMEFFAFHGHYEEEKLAGNKFVVDLTLWYNTRKAEQS
                     DNLEDALNYQSVYISVKEVISNTKVNLLEHLAAKILDTLFSEFDNLRKAEVYLKKVNP
                     PMGGQIKDVGVVMRKKNKKEKK"
     gene            5576..6004
                     /locus_tag="PKN53_01455"
     CDS             5576..6004
                     /locus_tag="PKN53_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87653.1"
                     /translation="MRTKLTFLVFLGAFFFLANTVEAQKVITAQKYVKSRGASEYVIP
                     SGDLAIEKPVLSRGGCLIKFDNWTGYYVDLWVDKVYKGRLNPWENSQLLLPEGYKEVY
                     CRTMGETYQWQSAGKCNEEFLLKLEEGSNAAATSTDSDEF"
     gene            6062..8089
                     /locus_tag="PKN53_01460"
     CDS             6062..8089
                     /locus_tag="PKN53_01460"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOD87654.1"
                     /translation="MKKIVLLIISIIFCVSFLKADKYALIIAIGEYDQELTGWYPISA
                     GNDVPLIERTLLDLGFPENNIMYLKDQDATKQGIESALAELYSKVSHGDQVVIHYSGH
                     GHQIFDDNGDEIDGLDEALVCFDAPMEMYEGYTGEKHFRDDELGDWVAKFRIKLGANG
                     HILLFLDSCHSGTGTRGSAKVRGGAPALVPAGWKPKNVEADDKVGFGISDKKAIASRG
                     VSTNDLAKFVVFSGASANELNYETFDDENNAVGSLSYCLFKSFCQMKEGDTYRQVFSR
                     IMAEMATKAPHQNPAIEGDIDYTVFGGDFYPQAGYFNLTSTKTDSLVEINGGRLSGIN
                     PGSIIVFVEQGTSNLNENTPIINSGKVLQTNNFFSQIRLDNKMKFDNNQSAWAFVKTR
                     TFTDQKLKVQIDDVMDADVKENLLTEIQKSNFVEITDDNPEIFLSYKKTRGAKVIGIT
                     NAHFDTELNKISNSNTESLNSDVVSTLNNYIQGKVIKDLDYYNEKYDVVMELIPVKAK
                     VYEDGNFDILEFYDANSILVNDVPEYNEDMMALIKVTNHGTHKSYFNIIDIQPDGFIN
                     PVIPTVNDIDGREYVLEPGETAILKNTLVMFGPPYGNEMFKLIAGDKPFNLSNTITGH
                     GNKTRGDSSNIFEAALQIGQTGTNTRGAKTVSSKTREDKISTYEYTFKIVE"
     gene            8159..9418
                     /locus_tag="PKN53_01465"
     CDS             8159..9418
                     /locus_tag="PKN53_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOD87655.1"
                     /translation="MKPLAERLRPKTFDDYVGQEHLVGKDAPLRKIVESGNIPSIILW
                     GPPGVGKTTLVNIIANLSEKDFYNLSAINSGVKDVREVIEKAKKSNLFSKSNPILFID
                     EIHRFSKSQQDSLLGSVEDGTLTLIGATTENPSFEVISPLLSRCQIYILKHLEEKDLD
                     KLIDRAINEDEELKKIKFTIKERDALYRFSGGDARKLYNILELLSSTAINNEITITNQ
                     FVVDKLQENINIYDKSGEQHYDIISAFIKSVRGSNPDAAVYWLARMLDAGEDIKFIAR
                     RLIILASEDIGLANPNALLLANACFDAISKIGMPEARIILSEITIYLATSPKSNSAYL
                     AIDKALALVKETGNLPVPLHLRNAPTQLMKNLNYGKDYKYPHDFPNNFIEQQYLPDKL
                     NSETIYEAGQNRKEKTITDYLNFLKNI"
     gene            9522..12824
                     /locus_tag="PKN53_01470"
     CDS             9522..12824
                     /locus_tag="PKN53_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOD87656.1"
                     /translation="MKKLSYILVLLFSTINLFSQSLVSLQEGRTNATIISNTQYSLSF
                     TSSLSGFDILGVKAKDGEYIRIVVPEYYPDKNVSNPELPVMTRLIEVPLDAEFEVRIK
                     SFDEQIVNLNEYGIDLQILPNQPSVSKSADYDKLPFYKNSSIYEDKEFYETQPVSVEL
                     LSKMRGIQIAQIRVSPFSYDIESNTLIIKNNIEAEIIYKNADFKKTAQLKADKYSPAF
                     GSAYNSLWNFKAPATKGAIDRYPIKYVIVSDRMFEAALNSFIKWKTKKGFYVTVAYTD
                     EIGTTATAIKAYLQGLYNAGTAEDPAPTYVLFVGDVAQVPYSKQVAGSGWLDEGHVTD
                     MYYCEYDGGTDYIPEVYFGRFSASTVAHLAPQIEKTLMFEQYTFPDPSYLGEAVLVAG
                     DDNSWASTHANGQVNYAHNYYFNADHGVTTLHKYLYPQSSQLAAEIRSKLGQGVGYAN
                     YSAHCDYDGWANPSFTISHIPSLNNEDKYFFSVGNCCLSNKFEVGECFGEALVRANKK
                     GAVAHIGGSNSTYWDEDFYWTVGLTSTITANVTYEQSGLGAYDHLFHDRGEDPYPSAY
                     EMIYAGNMAVEASTSSLKKYYWEIYHVMGDPSLMPYVGVPEQIQATYLSVMQIGMSSL
                     TVNTEENAYVALSNNGVLLDAVLANSSGIAVLNFETLTSTGNLDIVITKQFRAPLIEQ
                     IMVIPSDNDYDAMLSSIIAPQSQIHINNASLVPSIEIMNLGLLNLTSVNVGYILDEQA
                     PVTLNWTGNLAQLETAIVNFPEITLTSGNHTFTAFVSEPNEQTDQYPANDQKTQNVLV
                     YSGNVKIESIQSPEDKICNTSLLSPKVTIKNMDNEPLSSCVVSYVCGGITDEFTWSGN
                     LAQNETTQITFPENSFPDGNNTITFTIETVNGGGNSANFGASLEKNFKVISVANTIVF
                     DITSDYFPSHNAWSLTHNETSELIYSESLSNANEHHITELCLGDGCYTFEITDSNGDG
                     MNGWFLFPDKGSVKITNSTTSEVLWEYTSSGNWTSKTFEFCLDLTHAATRMISLNVFP
                     NPSTGIVNVESTYGISNICVYNNFGQIVENKNYKGQNIIEMDLQSLPNGVYIMKITDN
                     TEVTFKKIVLNK"
     gene            12923..13282
                     /locus_tag="PKN53_01475"
     CDS             12923..13282
                     /locus_tag="PKN53_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87657.1"
                     /translation="MKKICPVCNQEFEGRSDKKFCCDQCRNTYNNNKYADFSNQTRNI
                     NRILKSNRKILSDFFAKGVTKLNKERLLKAGYNFDYLTNIYTTRTGNTYHYCYDFGIT
                     NIEDNYFLIVEKKEYID"
     gene            13386..14804
                     /locus_tag="PKN53_01480"
     CDS             13386..14804
                     /locus_tag="PKN53_01480"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOD87658.1"
                     /translation="MIQLISKHKENENIVYIISKKTQVKKLELSNDEQIYVQKMFDNK
                     SELVHLNSLSFSKFVVLANEEKEINSRTEALRNLANKVANILNELKYESVNIIDFSDK
                     YLLDFAEALCLSTYSFNKYFKNKEEKSKSLKSINIISDKISKKEVLLKNSLCAGVFVA
                     RDIINEPASFMTAQKLGNEIVDLSKKYGFKAEVFDKAKIESLKMGGLLAVNRGSVEPP
                     TFSVLSYEPKNAVNKDYIVLVGKGLVFDTGGINLKPSGFLETMKSDKSGAAAVVGTIS
                     AIAKAKLPIKIIGLIPSTDNRPGNNAYVPEDIIKMFDGTTVEVMNTDAEGRLILADAL
                     AYAKKYNPELVIDLATLTGAAVVAIGESATVVMGNSEKHISELKKTGFEVWERLVEFP
                     LWDDYAEQLKSQVADMKNVGPRWGGAITAAKFLEHFTAYNWVHLDIAGTAFTEKDDSY
                     RGNGGTGVGVRLLFNYFLKILK"
     gene            14879..15946
                     /gene="pdxA"
                     /locus_tag="PKN53_01485"
     CDS             14879..15946
                     /gene="pdxA"
                     /locus_tag="PKN53_01485"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOD87659.1"
                     /translation="MSRLRIGITHGDINGISYEVILKTLSDSRLVNNITPIIYGSPKV
                     LAYYRKAFNNNSQTMTINSPEEARLKNINIINCNSEDVRVEMGLSTEMAGLAAYQALE
                     KAVQDLKDNKIDLIVTAPINKKNIQAAGFNFLGHTEFFAKEFNSKNHLMILMNDVIKI
                     GVVTEHIPISQVPSTLSKELILEKINTLNESLKSDFLIRKPRIAVLSLNPHCGDDGLI
                     GDEEKNIIIPAIEEAKTQDVLAFGPYPADGFFGAALYRNFDAVLAMYHDQGLAPFKVI
                     AFEDGVNYTAGLPIVRTSPAHGTAYDITGKNIASEQSFRSALYTAIDIYNNRNFQQEL
                     EEGKLNVDELENIKELKQNGE"
     gene            15946..17211
                     /locus_tag="PKN53_01490"
                     /pseudo
     CDS             15946..17211
                     /locus_tag="PKN53_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802942.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
     gene            17355..19139
                     /locus_tag="PKN53_01495"
     CDS             17355..19139
                     /locus_tag="PKN53_01495"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD87660.1"
                     /translation="MKKYVIILSVLSFTITNCVFAQTATQKFETAKELNDKQNFAEAI
                     IILEDLLADEFKNDEYFQELAYTYFNLLDFDKASEYYKKALELNKNCAICYANYSRAE
                     FEKGNFEKAESIANEGLELDANYSLLYMTRGLIYLATNRKELALSDFNKSIELDPDED
                     DYYITRTNYYIQINELYNAYSDVTKAIKLKPQNPQYYYYRAFILTNLKVHDEALIDID
                     KAIELNNNNVNYYNLKSTILMNWGKYEEAEIVIYKSLELVPDDYNAYLALSEVYLALS
                     SLERYCEVVDNAIDLCPTSELQKVEELKMWKNMYCFDDKLSYYTSRAFAYHLKSDYGN
                     AINISKQGVQKLGASSLLYDVMGSSYLARKEYELAKNCFDSCMNINELTVKEISECYG
                     LNISPQNAEIIAQTYHIRAEFGLAIIFLTKNDYKSAFLHIMNVINKAEKLENFANLEL
                     LYITKGLIYLGQKDIANAEAAFKIAREKNLYNPISRVNLAMLDVLKITNSKLKKLKFE
                     YEGVISCSRLIVPDVKLPKNFDLELLDPALEMCTFATEMSPKNPVPWLYLAKILQLKG
                     DPDYCKAAQNARDYGLYDSFTEIKADCK"
     gene            19154..20425
                     /locus_tag="PKN53_01500"
     CDS             19154..20425
                     /locus_tag="PKN53_01500"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF010697.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOD87661.1"
                     /translation="MKLDVSNSIKFFGENAFLRSKEYALKQLEVLKTGEGKGSEFLGW
                     VNLPQSINKTELNDIVEQAKSLQNLESVVIVGIGGSYLGAKAVIEALKPHFEQTKPEI
                     LFAGHTLNGNYHKELLEYLQNKEYGVIVISKSGTTTEPAIAFRLLYSDMKKRWDKKTI
                     RERLVVITDAKKGALRQLADKEKIKSYIIADDIGGRFSVLSAVGLLPIAVAGFDINEF
                     LKGASDAQNICLEPSLENPAIKYAAIRNLLYAMGHKIEIFVNYNHKLMYLAEWWKQLF
                     GESEGKENKGIFPASVSFTTDLHSLGQYIQQGERHLFETTIFIEEDSDFPAIPKDDEN
                     LDKLNYLAGKNMEYVNQKAAQATIMAHVEGKVPNMIIAINKLDEYNLGFLIYFFEVAC
                     GISAYMLGVNPFDQPGVEDYKKNMFKLLGKE"
     gene            complement(20523..20795)
                     /locus_tag="PKN53_01505"
     CDS             complement(20523..20795)
                     /locus_tag="PKN53_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87662.1"
                     /translation="MVKLTKELNADMTKATEDGVISDDEAKKINDSLKDFQNLMSKLE
                     KYENDKEVNEYLEKEGFESEVEKFAGAIVGLIMCEGADKIDLEGLM"
     gene            complement(20991..21365)
                     /locus_tag="PKN53_01510"
     CDS             complement(20991..21365)
                     /locus_tag="PKN53_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87663.1"
                     /translation="MKSNLFFIFIISFSLTIYSCKPTGPRQEVERLSKKIVEHTKILN
                     KAIEDKKITEQEAVEIKTAIIDLQKAFIQFDKKYKNDPDAQEIIQIYFKDKEAELEKI
                     YENYFTTLMEVYNCEGSEKVIF"
     gene            complement(21509..23218)
                     /locus_tag="PKN53_01515"
     CDS             complement(21509..23218)
                     /locus_tag="PKN53_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD87664.1"
                     /translation="MKKTLFFILATVTIFSLSSCNGLKKMIQNADDINYSVNPEILEM
                     HGGKVAVNITGNFPEKYFAKKVTATITPTLVWEGGEKALTPVYVQGEKIQGNAKVIQY
                     KAGGSFSYNDSFDYEPAMRRSHLELRIVGSQGKKTAEFDPEFIADGIVATPDLVKMQG
                     ASSVAKDKFIKDNPDVKVAAINYDKNRAELKNAETKKDEIVELKDFVNNVANDDRKEF
                     VNVELLSYASPEGTLEINTKVSNDRSKTVDKYVKNEFKKIDEFKNNDFFEHLVTEEDW
                     EGFKTVVQESNLADKDMILRVVNMNSDPDKREEEIRKMTATFEELEKLVHPLLRRSEI
                     KVNIMLIGHTDDEIKDLFKNSPEELTVEEFLYLGNLYDNDEDKLAVYTKASEVYNKEW
                     RCFNNLAAVQYRMKDYAAAKISIEKAKALDANATVFNNLGNVYLAEGNTSEAEKNYQS
                     ATGVPEASAGQGVLAIKNAQYKNAVDFYANMNCFNAGLARLLNGDSNGAIEAASNGKD
                     KEDALNYYLKAIAGARKGDTEVLFNNLRTACTKDSKLKDFASKDVEFIKYFENDTFKS
                     IVK"
     gene            complement(23326..23961)
                     /locus_tag="PKN53_01520"
     CDS             complement(23326..23961)
                     /locus_tag="PKN53_01520"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOD87665.1"
                     /translation="MSIRYLVIEGNIGAGKTSLCKKIAEDRNAKLILEQFAENPFLPK
                     FYENPERFAFPLELSFLADRYNQLKTNVENFDLFSDLIIADYFFMKSLIFSASTLQED
                     EYKLYRQLFDIIYSSLPKPDLYIYLHKSVENLQKNIKHRARDYEQNISSDYLKSIENG
                     YFNFFKQQTDMKIVLIDTNNIDFVNNEKHYKQIVEAIFEQNYLIGVNRIVF"
     gene            24210..25205
                     /locus_tag="PKN53_01525"
     CDS             24210..25205
                     /locus_tag="PKN53_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD87666.1"
                     /translation="MDTQIDIKELNEKIQRESSFIDVINFEMKKVIVGQKSLIEGLLI
                     GLLSDGHILLEGVPGLAKTLAINTLADVIDAKFARVQFTPDLLPADLIGTMIYSQKKE
                     EFFVKKGPIFANFVLADEINRAPAKVQSALLEAMQERQVTIGEISYPMEKPFLVMATQ
                     NPIEQEGTYPLPEAQVDRFMLKLKITYPEFEDERAIIRENLQGEMPKASKVLKPEDIL
                     KARSVVREVYLDDKIETYILDIVFASREPGKYGLPNLTNMIEFGGSPRASINLALAAK
                     AYAFIKRRGYVLPEDVRAVCHDVLRHRIGLTYEAEAENISSEEIISEILNSVEVP"
     gene            25254..26117
                     /locus_tag="PKN53_01530"
     CDS             25254..26117
                     /locus_tag="PKN53_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOD87667.1"
                     /translation="MEAKDLLKKVRKIEIRSRGLSSQVFAGEYHSAFKGKGMAFSEVR
                     EYQYGDPIKDIDWNVTARFSHPYVKVFEEERELTVMLLIDVSGSKAFGTSQKFKQELS
                     TELAAVIAFSAIQNNDKVGVILFSDKVEKFIPPKKGKKHILRIILELLDFEPESKGTD
                     VSQALKFFTNAIKKRSTAFIISDFMSTDFTDALKIAGNKHDLAALRVYDNFEKELPDL
                     GLAYFKDLETGQQKWVDTSLKNVRREYRINLQKQYDENTNLFKKSKLDFVDIRTDQDY
                     VKPLITLFKNR"
     gene            26136..27551
                     /locus_tag="PKN53_01535"
     CDS             26136..27551
                     /locus_tag="PKN53_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87668.1"
                     /translation="MKNILKYNILLILSLIFYTQAFSQEIKADIKLQSDTIIIGVPNE
                     LNISLRVDKTVEVFFPVFKDTISKNIELLGEFKPNIVDSEAFKEIQKKYLITSFDTGT
                     NIIPELRIKLVSGKDSSFVFTAETELFVKPYVLLDTIPVDTIYADNSGVVVFGKDAFK
                     NEIEQYIPDSIKQSVSVDSLNQIKSLVYEQLLSMYSSELLKNTGFNNQEEIKKIVDAT
                     SQNLFLVDKSGILNKYIVAGSVDTVFVQEFQQVQKGQALFTLYQIKDIKEDMLNTPFN
                     FAEFWYYFKKFLKDFWWALLALLVVVAGLIYFFKFYKKGVKPTFLKIKPEEPPHVIAL
                     RELEIIRKEKIWSKGQIKEYHVQITDVVREYIEKRFGVFAVEMTTSEILESFENNELI
                     SEADFMKLRQILELADNVKFAKYQALQNENDLSLKNSFDFVENTKEIIDENSKIIQTE
                     AVIEITQEENHEPTKDNNDDE"
     gene            27553..28536
                     /locus_tag="PKN53_01540"
     CDS             27553..28536
                     /locus_tag="PKN53_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOD87669.1"
                     /translation="MEFANKEYFWLFLIFIPMIVWYVWRLKKSVATMKISSLSNLKKN
                     PLPWRYILRHFLFGLRLLALSALIIVLARPQSMDRWENRKVEGIDIVIAFDVSGSMLA
                     EDFKPNRIEAAKEIGMEFVASRPDDRMGLVIFAGESYTQCPLTIDHAVLLNLFSEIKD
                     GIIEDGTAIGMGLATAVNRLKESDAISKVIILLTDGVNNMGNISPMAAAEIAKEFGIK
                     VYTIGIGKTGMAPYPFQTPFGIQYQNIEVKIDEEVLQNIASQTGGKYFRATDNQKLRD
                     IYKEIDSLEKTELEVKTYSQPREEFTLILMIAMLIFVVEQLLGLTILKNTF"
     gene            28538..29581
                     /locus_tag="PKN53_01545"
     CDS             28538..29581
                     /locus_tag="PKN53_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOD87670.1"
                     /translation="MFRFQNSEYLWLLLLLVVFIALFIIGLFIRKQKLKTAGDLKLIN
                     LLIPDFSLSRRIWKICLISLAWVFMVLALARPQFGSKLAEESTKGVEIIIALDVSNSM
                     LAQDLRPNRLESAKKFIERIISKLQNDKLGLIIFAGDAFVQMPITDDVRSARLFLSSI
                     STDLIPVQGTAIGQAIDLASKSFTENQDVSKVILLLSDGENHEDDALAASQFLTENRI
                     KVFTVSVGSKAGAPIPLKRGQGFLKDRNGNVVVSIPDEKVLVELANATDGIYVKLDNT
                     ANASDKIVDEISKLQQGEIKKQVYSEYDDQFTKFAALAFIFLIVEAVISERKNDIFRK
                     INLFKDKSNIKKS"
     gene            29587..30348
                     /locus_tag="PKN53_01550"
     CDS             29587..30348
                     /locus_tag="PKN53_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD87671.1"
                     /translation="MKFLKNIINIKTLSLLGLLTFCIVNLQAQEERKIIREGNKLYKN
                     KDYENAELNYKKALEINPELEKLHGNVSASQYRSGKFNDAVSSYQNVLKLLGNDKDKA
                     DAFYNIGNSYLQAGEYDKSIQAYKNAIRLNPEHDQSRYNMAVATKIQQQQNSGGDGQN
                     QDQNQDKQNDQNKDNKDNKDNQDKQDKQDDSKQDEKQDQNKDKEKQNQQQDNQMSREE
                     MERYLEALQQQEKDVQDKVNKEKIKTQKRVIEKNW"
     gene            complement(30372..31970)
                     /locus_tag="PKN53_01555"
     CDS             complement(30372..31970)
                     /locus_tag="PKN53_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HOD87672.1"
                     /translation="MLKKIPHTYVIVFFIIVFSAILTWIIPAGEFKREQKLMADGTTK
                     TMIVENSFEYVDSQPQTWQIFSSIFNGFVAQAHIIVFILLIGGAFWILNASKALDAGI
                     LSFIKATKKLERYKFLKKIGVNNIVMILIMTMFSLFGAIFGMSEETIAFVIVIVPLAI
                     SMGYDSIVGLGLVYVAAHIGFAGAILNPFTIGIAQGLSDLPLFSGWEYRVFTWLIINA
                     VGFTFILLYAKKIKKHPEKSLTYDIDDYWRKQKVNEDAKLTYYTPLSAWISFGILLIV
                     LILFSVFYPISKLSVGNAEFTFAALPVATALFAISSFFALRKSVHFYILNLLAFTVIF
                     LIIGVMGYDWYVMEIGTLFFAMGLAAGLAMNKSANDITKLFIEGAKDIFSAAFVVGLA
                     GGIIVILTDGKIIDTIMYGLSNMMASFGKETSLFSMYSIQTCLNLIIPSGSAKAALTM
                     PIMAPFSDLIGISRQATVLAFQFGDGFTNMITPASGVLIGCLGIAKVPYGVWFKFIWK
                     LVLIMFVLGFLLLLPTLYFNINGF"
     gene            complement(32059..32676)
                     /locus_tag="PKN53_01560"
     CDS             complement(32059..32676)
                     /locus_tag="PKN53_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87673.1"
                     /translation="MFSVVMLIVMLVAYSNTVKNKFKVKGERKIFTSYGGPFRRINGK
                     LIKDKKEIKKNVVPPVFFLELDGKPQAVKLRFSPPLRILRIILLALIILFLPVIIALF
                     LNGFDFERLELGGSAVWFCIVVIIVPVFLIKRYRIELYENPWIYIIKENGKEKRYRTK
                     SKITITKEDARSIGRLFYVPWGCLATWAIIIIFCMMCYLTAFGFD"
     gene            complement(32776..33384)
                     /locus_tag="PKN53_01565"
     CDS             complement(32776..33384)
                     /locus_tag="PKN53_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87674.1"
                     /translation="MPELIIPFRITEEEARNCLLDWCKANSQKKEAKNLEKKINLLKG
                     FYLPYEFVKGPVKSKVSRMDAYSSYNCGGYVDNVFINCSKQLDNMLLDGMEPFELDEL
                     QAFDFSYVAGHRVKVGDIDKNVLVERVEQEVSNDYASVVRKTLETKAIEVNTDTSSVF
                     RMPVLLPVYYICSGNEMAAVNGQTGKVSVRSIKRQTHYFIPW"
     gene            33371..33511
                     /locus_tag="PKN53_01570"
     CDS             33371..33511
                     /locus_tag="PKN53_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87675.1"
                     /translation="MSSGISSDKRKSFLTRERPQNGHWDRASPSSKTMVPPHPEQLAL
                     SK"
     gene            complement(33717..34790)
                     /locus_tag="PKN53_01575"
     CDS             complement(33717..34790)
                     /locus_tag="PKN53_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HOD87676.1"
                     /translation="MQSQEKYLLYDDTLKLYLAMLEDINQAKRSVYLQTYIFGNDSIG
                     QRFRQALTEAVRRGVEVKVLIDSWGTAVGQSFFKEFVELGGKLKFFEKIKVSFDVFSR
                     NHRRNHRKLLLIDSNITYIGSANITAYCLSWRECCLRIVSDMLCIVFEKIFSYDYYLS
                     SRFFQNKKYGTKILKGDEMAIVQDVPGTRKQPVKYVFRKLIDSAEKEVVIETPYFLPG
                     SLLKRSLINASKRGVKVTIIVPKNSDVTLFDILRNRYFGTYHKNGVEILEYRPSNLHA
                     KVFLSDNKYFFTGSSNFDYRSFLYMHEINIAGRQPDILRLLTEHIENTKKDCDKFNYE
                     KWKKRHGLQKLVETLLVPFRHLF"
     gene            complement(34884..35042)
                     /locus_tag="PKN53_01580"
     CDS             complement(34884..35042)
                     /locus_tag="PKN53_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HOD87677.1"
                     /translation="MAKKAVATLRTGGGQNLAKCIKMVKNPETGAYQFKETVTYKESA
                     KDFFNKKK"
     gene            complement(35051..35239)
                     /gene="rpmG"
                     /locus_tag="PKN53_01585"
     CDS             complement(35051..35239)
                     /gene="rpmG"
                     /locus_tag="PKN53_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOD87678.1"
                     /translation="MSKKAKGNRIQVILECTEHKESGMPGTSRYITVKNRKNTPDRME
                     LKKYNPILKKVTIHKEIK"
     gene            complement(35261..35497)
                     /gene="rpmB"
                     /locus_tag="PKN53_01590"
     CDS             complement(35261..35497)
                     /gene="rpmB"
                     /locus_tag="PKN53_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOD87679.1"
                     /translation="MARVCQITGKRAMVGNNVSHSKRRTKRVFDVNLTTKRFYDEETK
                     QWITLKVSTRGIKTISKKGLQAALRDARKKGFIN"
     gene            complement(35649..36350)
                     /locus_tag="PKN53_01595"
     CDS             complement(35649..36350)
                     /locus_tag="PKN53_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87680.1"
                     /translation="MEENKTLKEKNISILKETSRLKQEVYDKTLEAFGLLKNILQEIV
                     GEYNTDLEDVDDRVRLEFRDRGNFDAEIKIAGDVLIFNMHTNIFEFPRNHEIWKDQHY
                     IDQPLITYSGIINIYNFLSDSLKYNRVDDYGYLVGRLFINKENTFFVEGRRELGFNLP
                     VFGRNNVNPENFRTLIESAIAYALKFDLLVPPYNDVKIINVSYMNDKILKHQMQTGKR
                     LGFQYNAIKTNTDVK"
     gene            complement(36363..37835)
                     /gene="proS"
                     /locus_tag="PKN53_01600"
     CDS             complement(36363..37835)
                     /gene="proS"
                     /locus_tag="PKN53_01600"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOD87681.1"
                     /translation="MAKILTNKEENYSQWYNDLVIKADLAENSSVRGCMVIKPYGYSI
                     WEKMQATLDRMFKETGHVNAYFPLFIPKSFFSKEANHVDGFAKECAVVTHYRLRNAEG
                     GGVKVDPNAKLEEELIVRPTSETIIWNTYKNWINSYRDLPLLINQWANVVRWEMRTRL
                     FLRTAEFLWQEGHTAHATKEEAIEEAEKMIHVYEEFARDYLAIPVHIGTKTESERFAG
                     ADDTYCIEALMQDGKALQAGTSHFLAQNFAKAFDVKFLNQENKEEYVWATSWGVSTRL
                     MGALVMAHSDNNGLVLPPKLAPIQVVIVPIYKSKEELEKISERVHDLCKTLKTKGISY
                     KFDDRDSHRPGFKFAEWELKGVPIRLVIGPKDYENNTWEVARRDTFKKEIISNDKLLE
                     HIDFLLEDIQKSIYEKALKFREDNTYRVDDYEEFKRILEETPGFIEAHWDGTRETEEK
                     IKEETKATIRFIPFNPQLEEGKCIYSGKPSKFRVIFARAY"
     gene            37921..>38574
                     /locus_tag="PKN53_01605"
     CDS             37921..>38574
                     /locus_tag="PKN53_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87682.1"
                     /translation="MKAIFYILIIALLGMSSCTLNFYTTGGYDDVYLTGSEPKQGAIN
                     KQIVDEHKANYEETYNAEFNNTDDYYYDEYYDENGNLVQNDDGNDTYNYYGDTYNFND
                     YGYDLDDSYSYYFNNFRYYYPSYYYDPFYYSYYWDWGWGWGYRPYYCSYYYYPYYYYW
                     HRPYYYYGWGSSYWVGYNHGYTDGYWDGYWWGSNSNYIVDNNTYYIGHRGSSGGYGTG
                     "
     assembly_gap    38575..38674
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <38675..39677
                     /locus_tag="PKN53_01610"
     CDS             <38675..39677
                     /locus_tag="PKN53_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87683.1"
                     /translation="IIDKTPIDSKLEVSKAENSLSLSNSGKTPAQSGRNDVEPNTEEM
                     SGKKPVAVEGRINNIGVNKDNETGKQPIGVSGRVDNSSLSKGEVAAKQPTDAGRRVDN
                     SSRSEIPARQPIGVSSRADNPNLNRQVDPNAGKTQGSSSTPDNTGRVSTDNKPKEPAT
                     YNKPDLNSRNNYNQNTSGSRNNTDVRQQPSRNPSNVGQQPSRQPASRGGATPSQSYSR
                     PSNTNTGSFNSGSSSGRSSSSSSSAVRSNTNSNRSSYSTTPSSSRSSSTYNSRPSSSQ
                     RSSSSYNSGSSSSRSSGSSYSGSSSSSRSSSSSSSSSSRSGSSSSSGFGGSSSGGRR"
     gene            39698..41161
                     /locus_tag="PKN53_01615"
     CDS             39698..41161
                     /locus_tag="PKN53_01615"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOD87684.1"
                     /translation="MKKFLIITISVLISWTTLFAQNEIDALRYSYLIPGGTARYNAMG
                     GSFGALGADLSVMSFNPAGIGVYRSSELTFSTGFVLTQNYATINDNKASDFDFNFNIN
                     NISYLSSIKLKQNNSLKALNFGFAYNRLNNFNENIIIENPKSVNSMTDWFAARGNRTL
                     WSDLGAKDQFYSKLAWETYLIDQNLPDTLSYISAFNGKYGIEQKQSIYRSGNQGSYDF
                     SLSANIEDKLFVGGSLGITSVKFKELKRLEEVDTHDSIPEFNGFAFKEQLETSGSGIN
                     FKFGVLYSPVTWMRVGAAIHTPTFYKLRDKYSTQAESDFLDASMSLTESSPLGRFDYE
                     LSTPFRANASLGFILASKFLINVDYDYLDYSKAKLRSFDYGFYTENKNIEKAFDQAHN
                     LRLGLEYKIGGVALRTGVAYFDSPFKTGQVNQNAYYMTYNGGIGLRTNSMYFDIAYSY
                     INNEINYYMYQGLGVDSSRTLLKLQRHRIVLSLGFKF"
     gene            41357..42217
                     /locus_tag="PKN53_01620"
     CDS             41357..42217
                     /locus_tag="PKN53_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOD87685.1"
                     /translation="MRQLKITKSITNRESASLDKYLQEIGREELISVDEEVELAQRIK
                     QGDREALEKLTRANLRFVVSVAKQYQNQGLSLPDLINEGNLGLMRAAEKFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINKAFSKFEQENERKPTTEELAK
                     ALDLPEYKINDTLRVSGHHISVDAPFVQGEENSLLDVLENTDLPNTDAQLIKESLAKE
                     IERALATLTERERDIVKLFFGISCQEMTLEEIGDQFGLTRERVRQIKEKAIRRLRHSS
                     RSKLLKSYLG"
     gene            complement(42279..43088)
                     /locus_tag="PKN53_01625"
     CDS             complement(42279..43088)
                     /locus_tag="PKN53_01625"
                     /inference="COORDINATES: protein motif:HMM:NF016377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD87686.1"
                     /translation="MDDGNKIVNALWIGEHLSSVELLTIKSFLNNGHSFVLWAYDEIK
                     TELPKGVILKDANEIFPKSKVFSYKYSNQFGHGKGSFAGFSDIFRYKLLYLHGGWWTD
                     MDVTCLKPLDFETDYVFRTHHDLELVGNIMKCPKNSLLMKKCFEEAVEKVDENNRDWH
                     LPIQILVDNVKELGLTSYIRNFSNQDSWNLIRKFLSKNIEIPQEWYVIHWVNEEWRRN
                     RIDKHTFLKKSLFVKLADINNVSIKKAGFFKSLTIRFKLSKLGFGLKLFLK"
     gene            43217..44722
                     /locus_tag="PKN53_01630"
     CDS             43217..44722
                     /locus_tag="PKN53_01630"
                     /inference="COORDINATES: protein motif:HMM:NF033640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitch domain-containing radical SAM protein"
                     /protein_id="HOD87687.1"
                     /translation="MRQIYDFAKYYYQKLSRLKLPFMRYPDSKTKNFKSIPRSKMKEF
                     NKVRFHGPKKLACYNPFVNLFFNSRGQAVVCCRNQDIVLGTYPENTISEMWFGNVANK
                     LREHMLNNDLSMGCGYCEHQFKTSRFYGLPSMHADYYSSTKLTYPKIIELELSNICNL
                     QCVMCSGIVSSSIRKYREKQEPLKIVYDDKFIEQLKEFLPYAKEIKFYGGESFLIDNY
                     YKIWDFLLEIKSKAKLHVVTNGTTLNDKVKTYLKHLNFTITVSFDAMKKDLFESIRVG
                     ANFEKVKSNILEYNKILGGKGLSLSITPMKINCKEVPEIIDFCNDLNATVNMSYVENP
                     AEMALWTMPSKQLEELIEFYKFYKFKPIKSVNADYNFKAFSQFVNQISQYQKVNAKIE
                     KEYELTLKTKDECYQIISQTLDFVLRENIIFESDKTEIYKVIQEVETELSEDDKKFFY
                     GNLTNFLETKGVPALKNFVANGFDVKKLKDVLSSLSERPNIYIKNYKNISL"
     gene            44735..45877
                     /gene="dinB"
                     /locus_tag="PKN53_01635"
     CDS             44735..45877
                     /gene="dinB"
                     /locus_tag="PKN53_01635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOD87688.1"
                     /translation="MSSRRIVHVDLDSFYVSVERLLNSRLNGLPIIVGGNSDRGVVSS
                     CSYEARAFGVHAAMPIRKAKQLCPDAVFITGNMEKYSKYSNLVTDIIAENSPVYEKAS
                     IDEHYIDISGMDKFYSSLKWAQDLKSKIDKNTGLPISFAVSVNKTVAKIGTGEVKPLG
                     EICIEQAEVNNFLDPLAITKIPMLGKKTFEILSGIGLKTIKDIRETPVEKIVKLLGKN
                     GQSIWEKANGIDNSEVIPYQEQKSMSSETTFANDINDINKMLDIISEMAVNLAYKLRK
                     EELLTSTVTVKIRYSDFDTQTLQKQIPYSAFDHHLISHAKELFLKLYKRRAKVRLIGV
                     KFSQLVRGATQLNLFEDATEMTNLYKALDKIKNKYGEQIIKRGNSL"
     gene            46152..>46392
                     /locus_tag="PKN53_01640"
     CDS             46152..>46392
                     /locus_tag="PKN53_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87689.1"
                     /translation="MIIKTIIVTLGFLFVASLGFAQTQLSNDFNNATEMYVNTECIFE
                     TYRYNHDFGSDLPDLKCDRMYPGVSSYFIFKITENS"
     assembly_gap    46393..46492
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <46493..>48781
                     /locus_tag="PKN53_01645"
     CDS             <46493..>48781
                     /locus_tag="PKN53_01645"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOD87690.1"
                     /translation="KIQFETEKFFGLAFYTLQNGEYVELKCDTYKSTEGELKIYWDEG
                     LANVDIIARFWIMGELGSGEVDICLSSERQNLQPKVLNVSTTQYTVQELVQEVLITGC
                     LTASNITFTGNSAQIGYFSNAIPGLDFSSGVILSSGRVTDAPGPNTSGSTSTSFGGAG
                     DPQLNSLIPGYTTRDAAVLEFDFVPASDMLEFQYVFASEEYPEYAPPNTSTYNDVFGF
                     FISGGPENYNNVNVALIPGTVTPVSINNVNAVTNSQYYINNQGGADMEYDGFTVTFTA
                     SKPVTACATYHIKLAVADVGDMIYDSSVFLKENSFASGESYVVESYNAWSPSLSVMRG
                     CTNYVKFCRTTAIPLSQPVPVELFISGTAVMGVDYSAIPTNFQIPAGQECVIIEFDAF
                     DTGVLGDATIVLSFDSGCPCDIEMAEHVITLVDPYPVEATIIGSAPICVGETTSLSVD
                     ITTNDMTYVDIVWSTGVEDVTTINVSPTSTTTYTCNVIYPCDTITLETTVVVAQLPVV
                     DLGSDFSVEAVTANLNANMTPGNTGYWTVISGPGNATISPINSPNATVTVDTYGTYSF
                     SWTETSLAPNCVDSDIINVTFFHVPIADLEASPILCFGDNTIITFTGEAIPGFATFNW
                     DFGSGTIVDGTGQGPYIVNFGTPGSHSVSVTVTEAGMTSSNSVNVYVPYPISATLTTN
                     DASCYQTCDGSASVSVTGGTPPYTYSWGANVINNLCAGNYGLTITDNNACEYITSFTI
                     DEPSALTSSVTVQTNQICSTPGS"
BASE COUNT        17304 a         7090 c         7778 g        16409 t
ORIGIN      
        1 ggatgacgtg tttaatagcg gtagcacttg ggaagtgtaa taacaaaatt ttaaaataaa
       61 aaaaggctgc caaatttggt agcctttttt attgtttttt caaacagatt caaatattat
      121 tttatttcta gtgtagttgc aatatttcca aatggtacta tcattgggtc gtcagaatgt
      181 ggatcatcgc accaaactct accaaatttt ttatcaactt taacaacttt tgtatttaca
      241 aactttccaa cccatggtgc ttgaatttca tcacctactt taacatttgg tataatatct
      301 aaagcaacac aatcattttt atcataaatt ttcataatgc ctgcccagcc tattgtaagt
      361 actttatcgc cacttacatt aactattgtt gctactttat aatcattatt aactttaact
      421 gctacactag ttcctgcttc ccatttgttt tttaggacat ggaatgtgtt aggttcaatt
      481 ttttcagtct tttgaccaat acctacatca tcagtttttg caggattatt ccaatctata
      541 tctatatagt tcacttcagg ggctttggga tcagaagcat ttgtaacaat tgctcttttc
      601 ataccactgc ctgattgcca ccatgtaaga actatatcac caacttttgc agactcgtct
      661 ggtcttatag gtataatcat gtaatttggt atttcggtgt catcgtcaaa tatgaaatct
      721 ataacactgt attcatttcc tggctttgcc attttagccg aataaaaaat aaaagtctga
      781 gattcaggac ctttttttgt tgcatcaatt tgccagttaa gagatggagt taatacataa
      841 tcgccttctt gtgcaataat gttacctttt ggaaaatcct taaaagggtc aacttttaca
      901 acttcttctt ctggaacatc tgttaaaact tctttacttt cagcaggggt ttcttcatct
      961 gtttcatcac aagaaaaatc accgcctaaa tcaaaattgc taaacataag tatgttgagt
     1021 tgctcataag aagttgccat tctttttttt tgctttttca ttaaaagcct ttattttgtc
     1081 aggacattct ttttcaagtt ttttttcttg tttaccaaac tgtaccataa tagcaccaag
     1141 ttcttctagc tctttaaatg ctttttcatc accttttgta gctttttcta cagttgcgat
     1201 aaaaacatca attacttcat ttgcagcttt tatatactcg tcgcaatctg caaattcttt
     1261 accatcgtat ttttgcttta agtctgcaag gttttctgct tttttactac cacacgaaga
     1321 aagtccaaaa ataattaatg ctgaaacagt gaataacaat acttttttca taattataat
     1381 tctttagtta aacataaatt tgtttacaca aagataagct taatactttg ataatgctaa
     1441 tttattatgt aataaatcct gttttgtata aattgattaa caaatttggt gttttttatt
     1501 gtgttataaa ttttatttaa aaaatgaaat agcaaaaaaa aagctacata tggtagcttt
     1561 ttttttactt tttcatcaat attattcaac aacttcttct tctggaacat ctgttaaaac
     1621 ttcttcgctt tcaacatcaa catcttctgt ttcatcccaa gaaaaatcat cgcctaaatc
     1681 catttcacca aacataagtg tcataagttg aggtaaaaga gcttccattt ttttacttat
     1741 ttcttcttct aatgcctcta gcttttcagg acattcattt tcaaattttt cttgttgttt
     1801 gtaaaattgt tccataaatg cgtcaaattc ttcgaattct ttttttgcat tttcgtcacc
     1861 ttttgcagct ttttctacag ttgcgatata aatatcgatt atttcttttg cagcttcaat
     1921 atacttgtcg caatctgcaa attctttgcc gtcatatttt ttctttaaat ctgcaaggtt
     1981 ttctgctttt ttgttgcaag aagtaagtcc gaaaataatt aacgctgaaa tagcgaataa
     2041 taatactttt ttcataatta taatttttta attaaacata aatttattca acaaagataa
     2101 atataatgtt taataaaaac aaaatagaat aaaaaaaaag ctacacaagg tagctttttt
     2161 tatttttttt aaatgttatt cttattcaac aacttcttca gcaacttctt cagcaacttc
     2221 ttcagtcatt tcttcatcaa ctgcaacttc ttcttcatcc caagaaaaat catcgcctaa
     2281 atccatttca ccaaacataa gtgtcataag ttgagggaaa acagcttcca tttttttctc
     2341 tacttctttt tcaaaagctt ctaatttttc aggacattca gcttggaatt ttgcttcatc
     2401 accatcaaaa ttttccataa aagtagtaaa ttcttcgaat tctttttttg cagcttcatc
     2461 accttctgca gcttttttaa cagttgcgat atatacatct atgatttctg ttgaagcttc
     2521 gatgtactcg tcacaattag caaatgtttt gccttcatac tttgctttaa tgtcagcaaa
     2581 attttctgct ttctttttac cacaagaaga aagacctaaa ataattaccg ctgaaatagc
     2641 gaataataat acttttttca taattataat tttttaatta aacataaatt tattggtaca
     2701 aatataagac taaagtttta ataaaactaa ttttagcata agatttatta acatttttat
     2761 ttaaagtttt aacaaaatga atacaagcct tgttttaatt gcagttttat gaaggcaaaa
     2821 ccgacagtgt ttattttaaa tctgtttcat caatcccttt tggtaagaat tttgacaggt
     2881 cgcctttatt tttaataatg tctctaacta tactactaga aataaaagtg tgttctggta
     2941 aagtaagaat aaaaacggtt tcaattttat tttctaataa tttatttgtt tgtcctatag
     3001 ctctttcaaa ttcaaaatct gccgaagtac gtaagcctct caaaataaat ttgcaattat
     3061 tatttttaca aaaatcaact gttaatccag aataaatctt aacatctatt ttaggctcat
     3121 cggcaaaaac ttttttaatt attgcaagcc ttttttctaa acttaaaaac tcttttttac
     3181 tattattatc acctatgccg ataataattt tatcaaacaa aggtaatgct cttctaacaa
     3241 cagattcatg tccagcggta aaaggatcga aagagccagg aaaaattgca caattactaa
     3301 gtttatccat ttttagaaat tttagaccaa gaatctttta aatctacaat tctattaaac
     3361 accaattttt tatgactaga atctttatca acacaaaaat agcctttgcg ttcaaattgg
     3421 aatttgttac ctatttcagc attttcaaga tgtttttcta gtttggcact tattatttgt
     3481 aaagaatcag gattcaaata atcaaggtat gtttcgcctt cttctaaatc gtcaggattt
     3541 tcttttgtga aaagtctatc gtaaagtctt aattctgcat ctatacaagt ttttgaatca
     3601 acccaatgta ttgtgccttt tactttacgt ccgtctggag atttgcctcc tttactttct
     3661 ggatcgtaag tgcaatgaat ttctgttata ttaccatctt tatctttaac aacagattca
     3721 cattttatga aataagcgta acgcaatctt acttcgccac ctggcttcaa tctaaaaaac
     3781 ttgcttgggg catcttccat aaaatcatca cgctcaataa aaatttcgcg gctaaaagat
     3841 aaatttcttg ttccagcagt ttcgtcttca ggattatttt gagcttcaag catttcagtt
     3901 tttccctcag gataatttgt aataacaact tttataggat ctaaaactgc catcacacgg
     3961 tttgcttttt tattcaagtc ttcacgaata aaaaattcta acaaacctac atcaattatg
     4021 ttttcgcgtt ttgctacacc aattctgtct gcaaagttac gaattgattc aggagtatag
     4081 ccacgtcttc ttagaccact aattgttggc atacgagggt cgtcccaacc ttttacataa
     4141 tttcctttaa caagttctaa caatttacgt ttgctcatta ctgtataaga aagatttagt
     4201 cttgcaaatt caatttggcg aggacgtttt ccgcttgtat ctatttgatc caaaaaccaa
     4261 tcgtataaag gacgatgaac ctcgaactct aatgtgcaaa ttgaatgagt aattccttca
     4321 atatagtcgc tttgaccatg agcatagtca tacgttggat aaatgcacca tttatcgcct
     4381 gttctgtcgt gggattgttt cataattctg tacataacag gatctcgcat gtgcatatta
     4441 ggagaattca tatctatttt tgcacgtaaa accatgctac cctcgtcgtg ttttccagca
     4501 cgcatttctt caaacaaacg taagttttct tcaacactac gatttctata aggactctca
     4561 attccgggag tagtagggtt tgttctttgc aaacttatgt cttgaggagt ttgctcgtca
     4621 acataagcct taccatcttt tataagcata atagcccatt cgtaaagttg gtcaaaataa
     4681 tcggaagcat aaaacaagtt gtcatattca aaaccaagcc aatgaacgtc ttcaattatt
     4741 gaatttacat attcaagttc ttctttagaa ggatttgtat catcgaaacg taaatttgtt
     4801 ttgccattaa aagtttcttt taaaccaaaa tttaaacaaa ttgatttggc atgacctata
     4861 tgtaaatatc catttggttc tggtggaaag cgagtaataa tacttttgtg cttaccagaa
     4921 gctaaatcat tagtaataat ttgctcaata aaatttaagg cttctttttt ttcttccatt
     4981 tgataaaatt ttaaagtaca aagatataaa agttttagtt ttaaagattc gtaataattt
     5041 ataattttgc atacatatta attaataagt ataatacatt tcaaaaaatg aataaaattt
     5101 taattactgg aatggagttt tttgcttttc atggtcatta tgaagaagaa aaacttgctg
     5161 gaaataaatt tgttgttgac ctaactcttt ggtacaatac aagaaaagcc gaacaaagcg
     5221 ataacttgga agatgcactc aattatcagt cagtttatat aagtgttaaa gaagttatta
     5281 gcaatacaaa agtaaactta ttagaacatt tagcagctaa aattttagat actttatttt
     5341 ctgaatttga taatttgcga aaagcagagg tttatttaaa gaaagttaat ccaccaatgg
     5401 gcggtcaaat caaagatgta ggagtcgtga tgagaaaaaa gaataaaaaa gaaaaaaaat
     5461 aaaccaaaca ttgttttttt tgtcaaataa ttaatgtttt tggcacataa tttgctttta
     5521 agttagatat aaaaaaagag taaataaaat gaatagtaat ttaaaaaatt tagccatgag
     5581 aacaaagtta acattcttag tatttctagg agcatttttc tttttagcaa acactgtaga
     5641 agcacaaaag gtaataactg ctcaaaagta tgtaaaatct agaggagcat ctgaatatgt
     5701 tattccttct ggagatttag caattgagaa accagtatta tctcgcggtg gttgcttgat
     5761 taagttcgat aactggacag gttattatgt tgacttatgg gttgacaaag tttacaaagg
     5821 tcgtttaaat ccatgggaaa attcacaatt acttcttcct gaaggatata aagaagtata
     5881 ttgcagaaca atgggcgaaa cttatcaatg gcaatcagca ggaaaatgca atgaagaatt
     5941 cttgttgaag cttgaagaag gtagcaatgc tgcagctaca agtacggatt cagatgaatt
     6001 ttaaagaaaa aaattattac tgccactttt tagtggcagt ttttttaaaa aattagaaga
     6061 catgaaaaaa atagtattac taataataag tattatcttt tgcgttagct ttttaaaagc
     6121 tgataaatac gcattgataa tcgccatagg agaatacgac caagaactta caggctggta
     6181 ccctattagt gcaggaaatg atgtcccatt aatagaaaga accctgttgg atttaggttt
     6241 ccccgaaaac aatatcatgt atctaaaaga tcaagatgca acaaaacaag gtattgagag
     6301 tgcgttagca gaattatact caaaagtttc gcatggagac caagttgtaa ttcattatag
     6361 tggacatgga catcaaatat ttgatgataa tggtgatgaa attgatggct tagatgaagc
     6421 cctagtttgt tttgacgcac caatggaaat gtatgagggt tatactggtg aaaaacattt
     6481 tagagatgat gaacttggag attgggtggc aaaatttaga ataaaacttg gagcaaatgg
     6541 gcatattctt ttgttcttag actcttgcca ttctggaact ggaactagag gctctgcaaa
     6601 agttagaggc ggagcacctg ctttagtccc tgcagggtgg aaacctaaaa atgttgaagc
     6661 agatgataaa gtaggtttcg gaataagtga taaaaaagca attgcaagca gaggtgtatc
     6721 aacaaacgat ttagcaaaat ttgtagtttt ttctggagca agtgcaaacg aattaaatta
     6781 cgaaactttt gatgatgaaa ataatgctgt tggttcgctt tcgtattgtc tgtttaagtc
     6841 tttttgccaa atgaaagaag gagatactta tcgtcaagtg ttttcaagga ttatggcaga
     6901 aatggcaaca aaagcaccac atcaaaatcc agctattgaa ggagatattg attatactgt
     6961 gtttggtgga gatttctatc ctcaagcagg atattttaat ctaacttcta ctaaaacaga
     7021 ctctttagtc gaaattaatg gtggtagatt atctggaata aatcctggaa gtataattgt
     7081 ttttgtagaa caaggaacat caaatctaaa cgaaaataca cctattatta attctggtaa
     7141 agtattgcaa acaaataatt tcttctcgca aatacgatta gacaataaaa tgaaatttga
     7201 caacaatcaa tctgcttggg cttttgtgaa aactcgaaca tttaccgacc aaaaactaaa
     7261 agttcaaata gatgatgtta tggatgctga tgttaaagaa aatcttttaa cagaaattca
     7321 aaagtctaat tttgttgaaa taactgatga caatccagaa atatttttaa gctacaaaaa
     7381 aaccagagga gctaaagtaa ttggtattac caatgctcat ttcgatacag aattaaataa
     7441 aatatcaaat tcaaatacag aatctttaaa ctcagatgtt gtttcaactc tgaacaacta
     7501 catacaagga aaagtaatta aggatttaga ttattacaat gaaaaatatg atgtggttat
     7561 ggaattaatt ccagtaaaag ctaaagttta tgaagatgga aactttgata ttttagaatt
     7621 ttatgatgca aactcaattc tagtgaatga tgtaccagaa tataatgaag atatgatggc
     7681 attaatcaaa gttacaaatc acggaactca caaatcatat tttaatatta ttgatattca
     7741 gcctgatggt tttataaatc ctgttattcc aacagttaat gatatagatg gacgcgaata
     7801 cgtcttagaa ccaggcgaaa cagcaattct taaaaatact ttagtaatgt ttgggcctcc
     7861 ttatggaaat gaaatgttta aacttattgc tggcgacaaa ccttttaatt tgtcaaacac
     7921 aattactgga cacggtaata aaacaagagg tgatagttct aatatttttg aagcggcttt
     7981 gcaaatagga caaacaggaa caaatacaag aggagctaaa acagtaagtt ctaaaactag
     8041 agaagataaa attagtactt atgaatatac tttcaaaatt gtagaataaa atttactacc
     8101 tattatatgg ttttgattta aaaaatgttt ttgtattttt acaaaaacat ttttttttgt
     8161 gaaaccttta gctgaaagat taagacctaa aacttttgac gactatgtcg gacaagaaca
     8221 tttagtggga aaagatgctc ctcttagaaa aattgtagaa tcaggaaata taccatcaat
     8281 aattttatgg ggacctccag gtgttggaaa aacaacttta gtaaatatta ttgctaattt
     8341 aagtgaaaaa gatttttata atctgagtgc aataaattcc ggagttaaag atgttagaga
     8401 agtaatagaa aaagctaaga aatctaatct tttttcaaaa tcaaacccaa tattatttat
     8461 tgatgaaata caccgcttta gcaaatcgca acaagattca ttattaggct ctgttgaaga
     8521 tggtactcta acattaattg gagcaacaac agaaaatcct tctttcgaag taattagtcc
     8581 acttttatct cgttgccaaa tttatatctt aaaacatctc gaagaaaaag atttagataa
     8641 actcattgat agagcaataa acgaagatga agagcttaaa aaaattaaat ttacgataaa
     8701 agaaagagat gcattataca gattttcggg tggagatgct agaaaattgt acaatatttt
     8761 agaattattg agtagtactg ccataaataa tgaaataaca attactaatc agtttgttgt
     8821 cgataaatta caagaaaata taaatattta tgataaatca ggcgagcaac attatgatat
     8881 tatttcagct tttataaaat ctgtaagagg ttcaaatccc gatgcagcgg tttattggtt
     8941 ggctagaatg ttagatgcag gcgaagatat aaaatttatt gcacgaagat taattatttt
     9001 ggcaagcgaa gatattggac ttgcaaatcc aaacgctctt ctacttgcaa atgcctgttt
     9061 tgatgctatt tcaaaaatcg gaatgcctga agcaagaatt attttatctg aaataacaat
     9121 ttatttagct acaagtccca aaagcaattc tgcatatttg gctattgata aagctttagc
     9181 tttggttaaa gaaacaggta atttaccagt acctttgcac ctaagaaatg caccaaccca
     9241 attaatgaaa aatttaaact atggaaaaga ctacaaatat cctcacgatt ttccaaataa
     9301 ttttatagaa caacagtatt tgcctgataa attaaattct gaaactatat atgaggcagg
     9361 acaaaacaga aaagaaaaaa caattacgga ttatttgaat tttttgaaaa atatttgaat
     9421 atagaatact attaacttta atttaagtat taaaaataga aataatagaa tgaatgataa
     9481 tttttttgct taattttaca caaaatttaa aattatatct tatgaaaaaa cttagttaca
     9541 tattagtttt acttttttca accattaact tgttctcgca atcattggta agtttacaag
     9601 aaggcaggac aaatgcaaca attatttcaa acactcaata ttctttgagc tttactagtt
     9661 ctttatctgg ttttgatatt ttgggcgtga aagcaaaaga tggtgagtat attagaattg
     9721 ttgttccaga gtattatcct gacaaaaatg tctctaatcc agaattacca gtaatgacaa
     9781 ggcttataga ggttccttta gatgctgagt ttgaagttag aataaaaagt tttgatgagc
     9841 agattgtaaa cttaaatgaa tatggtatag atttacaaat attgccaaat caaccaagtg
     9901 tatcaaagtc tgccgattat gacaaacttc cattttataa aaattcaagc atttacgaag
     9961 ataaagagtt ttacgaaaca caacctgtaa gtgttgaatt actgagtaaa atgcgtggaa
    10021 tacaaattgc tcaaattaga gttagtccgt tttcttatga tatagaaagc aatactttga
    10081 ttattaaaaa taatattgaa gcagaaatta tttataaaaa tgcagatttt aaaaagactg
    10141 cacagctaaa agctgataaa tattctcctg catttggttc tgcatataat agtttgtgga
    10201 attttaaagc acctgcaacc aaaggagcaa ttgatcgcta tccaataaaa tatgtaattg
    10261 ttagtgatag aatgtttgaa gcagctctta attcatttat taagtggaaa actaaaaaag
    10321 gtttttatgt tactgttgca tataccgatg aaataggaac aactgctaca gctataaaag
    10381 catacttaca aggtttatat aacgcaggta cagccgaaga cccagcacct acttatgtcc
    10441 tttttgttgg tgatgttgca caagtacctt attcaaaaca agttgctggt agtggctggt
    10501 tggacgaagg gcatgttaca gatatgtatt attgcgagta cgatggtgga actgattata
    10561 tcccagaagt ttattttgga agattttcgg caagtactgt tgcacatcta gctcctcaaa
    10621 tagaaaaaac cttaatgttt gaacaataca cttttccaga tccaagttac ttaggcgaag
    10681 cagtattggt tgctggtgat gataattctt gggcatcaac tcatgcaaac ggacaagtta
    10741 attatgcaca taactactac tttaatgcag accatggagt tacaactttg cacaaatatc
    10801 tttaccctca atcatcgcaa ttagcagcag aaataagaag caaacttggg caaggtgttg
    10861 gctacgctaa ctatagtgca cactgcgact atgatggttg ggcaaaccct tcgtttacca
    10921 tttctcatat tccttcatta aacaatgaag ataaatactt ttttagtgtt ggtaactgct
    10981 gtttatcaaa taaatttgaa gtaggcgagt gttttggaga agcacttgtt agagcaaata
    11041 aaaaaggagc agtagctcat attggtggtt caaatagtac ttattgggac gaggattttt
    11101 attggacagt aggtttaact tctacaatta cagctaacgt aacttatgaa caatctggct
    11161 taggagctta tgaccattta ttccacgata gaggcgaaga tccatatcca agtgcttacg
    11221 aaatgattta tgcaggaaat atggcagtgg aagcatcaac ttcttcacta aaaaaatatt
    11281 attgggagat ttatcatgta atgggcgatc cttctttaat gccttatgtt ggtgtgccag
    11341 aacaaattca agctacatac ttatcggtta tgcaaatagg aatgagttca ttaactgtta
    11401 atacagaaga aaatgcttat gtggctttaa gtaataatgg agtgttactt gatgcagttt
    11461 tagcaaactc atctggcata gctgttttaa attttgaaac attaactagt actggaaatc
    11521 ttgatattgt aattacaaaa caatttagag ctccattaat tgaacaaata atggttatac
    11581 caagcgataa tgattatgat gcaatgctta gctcaattat cgcacctcaa agtcaaattc
    11641 atataaataa tgcaagcctt gttccttcta tagaaattat gaatcttgga ttgcttaatt
    11701 taacttctgt taatgttgga tatattttag atgaacaagc accagttacg ttaaattgga
    11761 caggtaattt agcacagtta gaaacagcta ttgtcaattt tcctgaaatt acacttacta
    11821 gtggtaatca tacttttact gcttttgtta gtgagccaaa tgaacaaacc gatcaatatc
    11881 cagcaaatga ccaaaaaact caaaatgttt tagtttattc tggaaatgtg aaaatagaat
    11941 ctatacaaag ccctgaagac aaaatttgta acacatcttt actaagtcca aaagtaacaa
    12001 ttaaaaatat ggataacgaa cctctaagtt cttgtgtagt ttcttatgtt tgtggcggaa
    12061 ttaccgatga gtttacatgg tcaggaaatc ttgctcaaaa tgaaacaacg caaattactt
    12121 tccctgaaaa tagtttccca gatggaaata atactattac ttttactata gaaactgtga
    12181 atggaggagg caattctgct aattttggag ctagtttaga aaaaaacttt aaagtcataa
    12241 gtgtcgcaaa tacaatagtg tttgatatca caagtgatta tttcccttca cataatgctt
    12301 ggagtttaac acataacgaa acatctgaac ttatttattc agaaagttta tctaatgcaa
    12361 atgaacatca tattacagaa ttgtgcttag gagatggctg ttatactttt gaaattaccg
    12421 atagtaatgg tgatggaatg aatggatggt ttttgttccc agataaaggt agcgttaaaa
    12481 ttactaattc aacaacttct gaagttttat gggaatatac cagcagtgga aactggacta
    12541 gtaaaacttt tgaattctgt ttagatttaa cacatgcagc aactcgaatg ataagtttga
    12601 atgttttccc aaatccaagt acaggaattg ttaatgtcga aagcacatat ggaatctcaa
    12661 atatttgtgt ttataataat tttggacaaa ttgtagaaaa taaaaactat aaagggcaaa
    12721 atattattga aatggattta caaagtctgc caaatggagt ttatattatg aaaataactg
    12781 ataatacgga agttactttt aagaaaatag ttttaaataa ataaacatta ctttaatata
    12841 attttgcaaa aagcccgatt tttaattggg ctttttgtat tttataaaag aaattactaa
    12901 atttgtgaaa aaagcataag taatgaaaaa gatttgtcca gtttgcaatc aagagtttga
    12961 aggaagatca gataaaaaat tttgttgtga tcaatgccgc aatacttata acaataacaa
    13021 gtatgcagat ttttccaatc aaacacgaaa tataaatcga attttaaaaa gtaataggaa
    13081 aattttatcc gatttttttg caaaaggtgt aacaaaactt aataaagaaa ggctgttgaa
    13141 agctggttat aattttgatt atttaacaaa tatttatact acaagaacag gaaatactta
    13201 tcattattgc tacgactttg gaattacaaa tattgaagat aattattttt taatagttga
    13261 gaaaaaagaa tatatagact aagtaaaagt tatttccaaa taatatttac tgcttttaaa
    13321 caaagttcta agtttttgtt tattaattta gaatattaat tttgtaacaa cttaaatttt
    13381 aaattatgat acaattaatt tcaaaacata aagaaaacga aaatatcgtt tatattatct
    13441 caaaaaaaac tcaggtaaaa aaacttgaat taagcaatga cgagcaaatt tatgtacaaa
    13501 aaatgttcga taataaatct gaattagttc atttgaactc tttgagtttt tcaaaatttg
    13561 tagttttagc aaacgaagaa aaggagatta attctaggac agaagctttg agaaatttgg
    13621 caaacaaggt agccaatatt ttaaatgaac ttaaatatga aagcgtaaac attattgatt
    13681 ttagtgataa atatttgtta gattttgcag aggcattgtg tttaagcaca tactcgttca
    13741 ataaatattt taaaaataaa gaagaaaaat caaaaagttt gaaatcaata aatattatca
    13801 gtgataaaat ttctaaaaaa gaggttttgc taaaaaattc tttatgtgct ggagtttttg
    13861 ttgctagaga tataattaat gagcctgctt catttatgac tgctcaaaaa cttggtaatg
    13921 aaattgtgga tttgtcaaaa aaatatggtt ttaaagctga agtttttgat aaagcaaaaa
    13981 tcgaaagttt aaaaatgggc ggacttttgg cagtaaatcg tggaagtgta gaaccaccaa
    14041 cattttcggt attgagttat gaacctaaaa atgcagtaaa taaagattat atcgttttgg
    14101 ttggaaaagg tttagttttt gatactggag gaattaattt gaaaccaagt ggttttttag
    14161 aaacaatgaa aagcgataaa tctggtgctg ctgcagtagt aggaactatt tcagcaatag
    14221 caaaagcaaa attgccaatt aaaattattg gtttaattcc atctaccgac aacagaccag
    14281 gaaataatgc ttatgtgcca gaagatataa ttaaaatgtt tgatggcact actgtagaag
    14341 ttatgaatac tgatgcagaa gggcgactaa ttttagccga tgctttagct tacgcaaaaa
    14401 aatataatcc cgaattagtt attgatcttg caacgcttac tggtgctgca gttgttgcta
    14461 ttggtgaaag tgcaacagta gtaatgggaa attcagaaaa acatatttca gaacttaaaa
    14521 aaactggttt tgaagtttgg gaaagacttg tagagtttcc tctttgggac gattatgcag
    14581 aacaattaaa atctcaagtt gctgatatga aaaatgttgg accacgctgg ggcggagcaa
    14641 ttacagcagc aaaattttta gaacatttta ccgcttataa ttgggtgcat ttagatattg
    14701 caggaacagc ttttacagaa aaagacgatt cgtatcgagg aaatggcgga acaggcgttg
    14761 gcgttagatt actttttaat tatttcttaa aaatattgaa ataaactgaa agaaagtaag
    14821 atatttttag tgaaaatagt ttaaaaaaat taactttgta agaattaaaa aataaaagat
    14881 gagcagatta agaataggaa ttactcatgg tgatataaac ggaattagct atgaagtgat
    14941 tttaaaaaca ttatcggatt caagactagt aaataatatt actccaataa tttatggttc
    15001 gccaaaagtt ttggcatatt atcgaaaggc atttaataac aacagccaaa cgatgacaat
    15061 caactctccc gaagaggcaa gactgaaaaa tattaacata atcaactgca atagcgaaga
    15121 tgttagagta gaaatgggct tgtccacaga aatggcaggt ttagcagctt atcaggcatt
    15181 agaaaaagca gttcaagatt taaaagataa taaaattgac ttgattgtaa ccgctccaat
    15241 taataaaaaa aatattcaag cagctggatt taatttcctt gggcatacag agttttttgc
    15301 taaagaattt aattcaaaaa atcatttgat gattttaatg aatgatgtga taaaaatagg
    15361 agtggtaacc gaacatattc ctataagtca ggttccttcg actttaagta aagaattaat
    15421 tttagaaaaa ataaatactt taaacgaaag tttaaaaagt gattttttaa ttagaaaacc
    15481 acgaattgca gtgcttagct taaatcctca ttgtggcgat gatggtttga ttggagatga
    15541 agaaaaaaat attataatcc ctgcaataga agaagctaaa acacaagatg tacttgcatt
    15601 tggaccttat ccagccgatg gatttttcgg agctgcatta tatagaaatt tcgatgcagt
    15661 tttagcaatg taccacgatc aaggtttagc accatttaaa gtaatagctt ttgaagacgg
    15721 agttaattat acagcaggtt tgccaatagt aagaacttca ccagcacatg gcacagctta
    15781 tgatattaca ggcaaaaata ttgcttctga acaatctttc agatctgcat tatacacagc
    15841 aatagatatt tataacaata gaaattttca gcaagaactt gaagaaggaa aattaaatgt
    15901 tgatgaatta gaaaatatta aagaattaaa acaaaacgga gagtaatggc aatttttaca
    15961 aaaaaagaag ccctagaata tcacagcaat ggtagaaaag gaaaagtaga agtagttcct
    16021 acaaaatcat ttcaaacaca aaaagattta tctttagcat actcaccagg agttgcagaa
    16081 ccttgcttag aaatagaaag agacaataaa accgcttacg attatactgc aaaaggaaat
    16141 cttgttggtg taatttcaaa cggaacagct gttttaggat tgggcgatat tggagcttcc
    16201 gcatcaaaac cagtaatgga aggaaaaggg tttttgttta aaatttttgc cgatgtagat
    16261 gtttttgata ttgaaatcaa tgaaaaaaat ccagaaaaac ttatagaaat tgttaaaact
    16321 ttagaaccta cttttggagg gataaattta gaagatatta aagctccaga atgttttgtt
    16381 attgaagaaa gattaaaagc agaaatgaat attccagttt ttcatgacga ccaacatgga
    16441 acagcaataa ttgtaggtgc aggtttatta aacgctttaa aattagtaaa taaaaaaatt
    16501 gaagacgtta aagttgtgtt taatggtgct ggagctgcgg gtatagcttg tgctagattt
    16561 cttctaagtt taggcgtaag aaaagaaaat attgtgcttt gcgatttaaa aggagttgtg
    16621 agacacgaca atgttggaaa taatatttat caaaaagaat ttgtaacaga tagaaacata
    16681 agtgctttat cagaagctat ggtcggtgca gacgtgttta taggtgtttc agtagcaaat
    16741 gttgttagta aagaaatgct taaacaaatg gctgaaaatc ctattgtgtt tgctctagca
    16801 aatccaaatc ctgaaatttc ttataacgat gcaaaaaacg caagacctga tgtgattatg
    16861 gcaacaggaa gaagcgatta tccaaaccaa gttaataatg ttttaggctt tccttttatt
    16921 tttagaggtg ctttagatgt acaagcaact tgtataaacg aagaaatgaa aatcgcagct
    16981 gctaaagctc ttgcagaatt ggctttgttg cctgtcccag aatatgttag caaagcttat
    17041 ggacaagaaa aaatagagtt tggaaaagaa tatattatcc caaaaccatt agacctaaga
    17101 gttattgaat gtgtcgcagt agcagtagca aaagcagcaa ttgatacaaa agtggcaaat
    17161 aaaactatag aaaattggga aacttataaa gcagaattaa gaaaacgagt agaaagagta
    17221 agaaaaaact attctagctg taatttttag tgttgacgaa aaaaatataa aatatatttt
    17281 ataaatttgt agtattaaaa ctttataact taaattagta gcaattaata gaagttattt
    17341 aaaaaattat agctatgaaa aaatatgtga taattttaag tgtactaagt ttcacaatca
    17401 ctaattgtgt atttgcacaa actgcaactc agaaatttga aactgcgaag gaacttaacg
    17461 acaaacaaaa ttttgcagaa gcaataataa ttttagaaga tttacttgct gatgaattca
    17521 aaaatgatga gtattttcaa gaattagctt atacttattt taatttattg gattttgata
    17581 aagcaagtga atattataaa aaagcattag aacttaataa aaattgtgca atttgttatg
    17641 caaattattc gcgtgcagaa tttgaaaaag gaaattttga gaaggctgaa tctattgcta
    17701 atgagggctt ggagcttgat gctaactatt cgctactcta tatgacacga ggtttgattt
    17761 atttagcaac taacagaaaa gaacttgctt tatcagattt taataaatct attgaattag
    17821 atcccgacga agatgattat tatattacta gaacaaatta ttacattcag attaatgaat
    17881 tatataatgc ttatagtgat gtaactaagg caataaaact aaaaccgcaa aacccacaat
    17941 attattatta tagagcattt atacttacaa atttaaaagt tcatgacgaa gcacttattg
    18001 atattgataa agctatagaa ctaaataaca acaatgtgaa ttattacaat cttaagagta
    18061 ctattcttat gaattggggg aaatatgaag aagcagaaat agtaatttac aaatctttag
    18121 aattagtgcc agatgattat aatgcatatt tggctttgtc agaagtttat ctagcattaa
    18181 gtagtttaga acgttattgt gaagttgtcg ataatgcaat tgatttatgc ccaacttcag
    18241 aacttcaaaa ggtagaagaa ttaaaaatgt ggaaaaatat gtattgcttt gatgataaac
    18301 tttcttatta tacatcacgt gcttttgcat atcatttaaa aagtgattat ggaaatgcta
    18361 tcaacatatc aaaacaaggt gttcaaaaac ttggtgcttc atcacttttg tacgatgtta
    18421 tgggctcatc ttatttagct cgtaaagaat atgagcttgc taaaaactgt tttgactctt
    18481 gtatgaatat taacgaactt acagtaaaag aaatatcaga atgttacggc ttaaacatat
    18541 ctcctcaaaa tgcagaaatt atagcacaga cttatcatat tagagcagag tttggattgg
    18601 caataatatt tttaactaaa aacgattata aatcggcttt tttacatatt atgaacgtaa
    18661 ttaataaagc cgaaaagtta gaaaattttg ctaatctaga gttgctatat attacaaaag
    18721 ggttaattta tttaggacaa aaagatatag ctaatgctga agctgctttt aaaatagcaa
    18781 gagagaaaaa tctttacaac ccaatatcta gagtaaattt agctatgtta gatgtgttaa
    18841 aaataacaaa ttctaaatta aaaaaattaa aatttgaata tgagggagta atatcttgtt
    18901 caagattaat tgtacctgat gtaaaattac ccaaaaattt tgatttagaa ttattagacc
    18961 cggctttaga aatgtgcaca tttgctacag aaatgagtcc gaaaaatccc gttccttggt
    19021 tgtatttagc aaaaatactt caactaaaag gagacccaga ttattgtaaa gctgcacaaa
    19081 atgctagaga ttacggtttg tatgattcgt ttacggaaat aaaagctgat tgcaaataat
    19141 aaaactaaaa tatatgaaat tagatgtatc aaattcaata aaattctttg gagaaaatgc
    19201 ttttctacgc tcaaaagaat atgctcttaa acaattagaa gtattaaaaa caggggaggg
    19261 taaaggctcc gaatttttag gttgggtaaa tttaccgcaa tctattaata aaacagaatt
    19321 aaatgatatt gttgaacagg caaaaagtct tcaaaattta gaaagtgttg taattgttgg
    19381 tattggtggt tcatatctag gagcaaaagc agtaattgaa gctctaaaac ctcattttga
    19441 acaaacaaaa ccagaaattt tatttgccgg gcatacttta aatggtaatt atcataaaga
    19501 attgttagaa tatttgcaaa acaaagaata tggagttatt gtaatatcaa aatcaggaac
    19561 tacaacagaa ccagcaattg ctttcagact tttgtatagc gatatgaaaa aacgctggga
    19621 caaaaaaaca attagggagc gtcttgttgt aattacagat gcaaaaaaag gagctttacg
    19681 ccaacttgca gataaagaga agattaaaag ttatattata gctgatgata ttggaggtag
    19741 attttctgtt ttgtcagcag ttggactttt acctattgca gtagcaggat ttgatatcaa
    19801 tgagttttta aaaggagctt ctgatgccca aaatatttgc ttagagccaa gtttggaaaa
    19861 cccagctata aaatatgctg ctattagaaa tttactttat gcaatgggac ataaaatcga
    19921 aatttttgta aattataatc ataaattaat gtatttagca gaatggtgga agcaactttt
    19981 tggagaaagt gaaggaaaag aaaataaagg tatttttcca gcatctgtgt cttttacaac
    20041 cgatttacac tcacttggac aatatattca acaaggagaa aggcatctat ttgaaactac
    20101 aatttttatt gaagaagact ctgactttcc tgcaattcca aaagatgatg aaaatcttga
    20161 taaattaaat tatttggcag ggaaaaatat ggaatacgta aatcaaaaag ctgctcaagc
    20221 aactattatg gctcatgttg agggaaaagt tccaaatatg attattgcta ttaataaatt
    20281 agatgaatat aatcttggct ttttaattta cttcttcgaa gttgcttgtg gaattagcgc
    20341 gtatatgctt ggagtaaatc cattcgacca accaggagtt gaagattata aaaagaatat
    20401 gtttaaattg cttgggaaag agtaggaatt taaaaacttt atttgaagca tatatataaa
    20461 aaaggctgtc ttaaaaattt attttttggc agcctctttt tataacttat aaattaattt
    20521 ttttacataa gaccttctaa atcaatttta tcagcacctt cgcacataat tagacctaca
    20581 attgctccag caaatttttc aacttctgat tcaaatcctt ctttttcaag atattcattg
    20641 acttctttat cgttttcgta tttttctaat ttactcataa gattttgaaa atctttcaat
    20701 gaatcattga ttttttttgc ttcgtcatca ctaattactc cgtcttcagt tgctttagtc
    20761 atgtctgcat taagttcttt agttagtttt accaattttt cagcctctcc aacaggacta
    20821 ctatactaaa catagagcaa atgcaacaat tgctactaat aatccaattt ttctcataat
    20881 ttttaattta attttgttaa tactaatttt atctgttaag gaactacaat tatttgtaaa
    20941 aagcaaattt ttttaaaata ttttattttt tatgaaataa tttaaaattc ttaaaaaatt
    21001 actttttcac ttccttcaca attatacact tccattaatg ttgtaaagta attttcatat
    21061 attttttcta attcagcttc tttatctttg aagtaaattt gaattatttc ttgagcatct
    21121 ggatcatttt tgtatttttt atcaaactgt atgaaagctt tttgtaaatc aataatagct
    21181 gtttttattt ctacagcttc ttgttctgtt atttttttat cttcaatagc tttgtttaag
    21241 atttttgtat gttcaactat tttttttgag agtctctcaa cttcttgtct aggtccagtt
    21301 ggtttgcacg aatatattgt aagtgaaaaa ctaataataa aaataaaaaa taaattagac
    21361 ttcataaagt ttaatttgtg ttaaaaaatt taaaagtaaa gatataaaat atttttgttt
    21421 tgtgaataaa ctgcaatggt ttaaacagtg gtaaataaaa aaagctgtct tattaggaca
    21481 gcttttttcg agttttatga aaacaaattt attttactat tgatttgaaa gtatcgtttt
    21541 caaaatattt aataaattca acgtctttgc ttgcaaaatc ttttagttta gaatctttag
    21601 tacaagctgt tcttaagttg ttgaataaaa cttcagtgtc acctttgcgt gcgccagcga
    21661 tagcttttaa gtaatagttt aaagcatctt ctttatcttt accattactt gctgcttcaa
    21721 tagcaccgtt tgaatcaccg ttaagaagtc ttgctaaacc agcgttaaag caattcatgt
    21781 ttgcataaaa atcaacagca tttttgtatt gagcattttt aattgctaat acaccttgtc
    21841 ctgcacttgc ttctggaaca cctgtagctg attgataatt tttttctgct tcactagtat
    21901 taccttctgc tagataaacg tttcctaagt tgttgaatac agttgcgtta gcatctaaag
    21961 ctttagcttt ttctatactg atttttgctg cagcgtaatc tttcattctg tattgaacag
    22021 cagctaagtt attaaaacat ctccattctt tattgtaaac ttcgctagct tttgtataaa
    22081 cagctaattt gtcttcatca ttgtcgtata agttacctaa gtataagaat tcttcaacag
    22141 taagttcttc tggagaattt ttgaataagt ctttaatttc gtcatcagta tgaccgataa
    22201 gcatgatgtt aactttaatt tcagaacgac gtaatagagg gtgaacaagt ttttcaagtt
    22261 cttcgaaagt agctgtcatt ttacgaattt cttcttcgcg tttgtctggg tcagagttca
    22321 tgtttacaac acgtaaaatc atgtctttgt cagctaaatt agattcttga actactgttt
    22381 taaagccttc ccagtcttct tctgtaacta aatgttcaaa gaaatcattg tttttaaatt
    22441 catcaatttt tttgaactca tttttaacat atttgtcaac agttttagat ctgtcattag
    22501 aaacttttgt attaatttct aatgttcctt caggtgaagc ataagataaa agctcaacgt
    22561 ttacaaattc tttgcgatcg tcatttgcaa cattgtttac aaaatctttt aattctacaa
    22621 tttcatcttt ttttgtttct gcatttttca attctgctct atttttatca tagttgatag
    22681 cagcaacttt cacatcagga ttgtctttta taaatttatc tttagcaact gaactagcac
    22741 cttgcatttt tacaagatct ggagttgcaa caataccgtc tgctataaat tcaggatcaa
    22801 attcagcagt ttttttacct tgactaccta caatgcgaag ttctaagtgt gatcttctca
    22861 tagcaggttc ataatcaaaa ctatcattgt aagaaaaact tccaccagct ttatattgaa
    22921 ttactttagc atttccttgg attttttcac cttgcacata aactggagtt aaagcttttt
    22981 cgccaccttc ccaaactaaa gttggtgtaa tagtcgcagt tacttttttt gcgaaatatt
    23041 tttcagggaa attgcctgta atattaacag caactttgcc tccgtgcatt tctaaaattt
    23101 ctggatttac tgagtaatta atatcatcag cattttgaat catttttttc aaaccattac
    23161 agctactaag tgaaaaaata gtaactgtgg ctaaaataaa gaataaggtc tttttcataa
    23221 tttaaactct tttatttatt taacaattaa attttttaaa cattatttat gcaaaaataa
    23281 taagtttttc acaaaaaatc gcataaaatt cgtttttttt tcattttaaa atacaattct
    23341 attaacacct attaaataat tttgttcaaa aatcgcttca actatctgtt tgtaatgctt
    23401 ttcgttgttt acaaaatcaa tattgtttgt gtctattaaa actattttca tgtcagtttg
    23461 ttgcttaaaa aaattaaaat atccgttttc tatgcttttt aaatagtcag aactgatatt
    23521 ttgctcgtaa tctcttgctc tatgtttgat gtttttttgc aaattttcta cagatttatg
    23581 taaataaata tataaatctg gtttgggaag cgaactgtaa attatgtcga aaagttgacg
    23641 atatagtttg tattcatctt cttgtaaagt tgaagccgaa aaaatcaaag atttcataaa
    23701 aaaataatct gcaatgatta agtcggaaaa caaatcaaaa ttttcgacat tagtttttaa
    23761 ttgattatac ctgtcggcta aaaaagataa ttctaatgga aaagcaaatc gctcaggatt
    23821 ttcatagaat tttggcaaaa acggattctc ggcaaattgc tctaaaataa gctttgcatt
    23881 gcggtcttca gcaattttct tacataaaga agtcttgccc gcacctatat taccttctat
    23941 aactaaatag cgaatactca ttagccaaag gtaaaacttt ttattaaatt ttaaaactaa
    24001 ataattaaaa aattaatgtg tttaaaaaat taaatttaat agcaaagctc gaaacatcaa
    24061 cagcacaaag ctttgtaaca aaatttaatt aacagcgtat taacagtttt aacaaaaaac
    24121 taagataaaa acatctaaga aggcttgata aaaatcaatt aaaattatat tttcgtaaca
    24181 ttgtttttat aaagttaaaa aatttaatta tggatactca aattgatatt aaagaattaa
    24241 atgaaaagat acaacgagaa agtagtttta ttgatgtaat caactttgaa atgaaaaaag
    24301 taatcgttgg tcaaaaaagt ttaattgaag gtttattaat aggtttatta tctgatggac
    24361 atattttgtt ggaaggtgta ccaggtttgg caaaaacctt agcaatcaac acattagccg
    24421 atgttattga tgcaaagttc gcaagagtgc aatttactcc agatttatta cctgccgact
    24481 tgattgggac aatgatttat agtcagaaaa aagaagaatt ctttgttaaa aaaggtccta
    24541 tttttgcaaa ttttgtttta gcagacgaaa ttaatcgtgc cccagctaaa gtccagtcag
    24601 ctttgttaga agctatgcaa gagagacaag taaccatagg agaaattagc tatccaatgg
    24661 aaaaaccttt tttagttatg gcaactcaaa atcctattga gcaagaagga acttatcctc
    24721 ttcccgaagc tcaagttgat agatttatgc taaaactaaa aattacttat ccagagttcg
    24781 aagacgaaag agctatcatt cgcgaaaatt tgcaaggaga aatgccaaaa gcttctaaag
    24841 ttttaaaacc tgaagatatt ctaaaagctc gttctgttgt tagagaagtg tatcttgatg
    24901 acaaaatcga aacttatatt cttgatattg tatttgcaag tagagaacca ggaaagtacg
    24961 gacttcctaa cttaacaaat atgatagaat ttggtggttc gccacgtgca tctattaatt
    25021 tagctttagc agcaaaagct tatgctttta taaaaagaag aggctatgtg ttgccagaag
    25081 atgttcgtgc tgtttgccac gatgtgctta gacatagaat tggtttaact tacgaagccg
    25141 aagcagaaaa tattagttca gaagaaatta ttagcgaaat attaaactct gtcgaagttc
    25201 cataatagaa aacaataaaa taaaaattgt tttaatgtta aaatgaatat ttagtggaag
    25261 caaaagattt attaaaaaaa gttagaaaaa ttgaaattcg ctcgcgaggg ctgtcttctc
    25321 aagtgtttgc aggagaatat cattctgcct ttaaaggaaa gggaatggct ttctccgaag
    25381 taagagaata tcagtatggc gacccaatca aagatataga ttggaatgta acggcaagat
    25441 ttagccatcc ttatgtgaaa gttttcgaag aagaacgcga acttactgtt atgttgctga
    25501 tagatgttag tggctcaaaa gctttcggaa cttcacaaaa atttaaacaa gaacttagta
    25561 cagaattagc tgcggtaatt gctttttcgg ctattcaaaa taacgacaaa gtgggggtaa
    25621 tacttttttc ggataaagta gaaaaattta ttccacctaa aaaaggaaaa aaacatatac
    25681 taagaataat tcttgaatta ttagattttg aaccagaaag taaaggaaca gatgtttcgc
    25741 aagctttaaa attctttaca aatgcaatca aaaaaagaag tacagctttt ataatttctg
    25801 actttatgtc aacagatttt accgatgcgc ttaaaattgc tggtaacaaa cacgacttgg
    25861 cggctcttag agtgtatgac aatttcgaaa aagaattgcc agatttaggt ttagcttatt
    25921 ttaaagattt ggaaacagga caacaaaaat gggttgatac ttctttaaaa aacgtaagaa
    25981 gagaatatag aataaatttg caaaaacaat atgatgaaaa tacaaattta tttaaaaaat
    26041 caaaattgga ttttgtcgat attagaaccg accaagacta tgttaaacca ttgattactt
    26101 tgtttaaaaa tagataaaat ttgaatttct ataaaatgaa aaatatttta aaatacaata
    26161 tacttctaat tcttagtttg attttttata ctcaagcatt ttcgcaagaa atcaaagcag
    26221 atataaaact tcaaagcgac actattataa taggtgtgcc aaacgagtta aatataagtt
    26281 tgcgtgtaga taaaactgta gaagtatttt ttcctgtctt taaagatact atttcaaaaa
    26341 atattgaact tttaggtgaa tttaaaccta atattgttga ttctgaggct tttaaggaaa
    26401 tacaaaaaaa atatttaatt actagttttg atacaggaac aaatattatt cccgaactaa
    26461 gaataaaact tgtttcagga aaagatagta gctttgtttt tactgctgaa acagaattgt
    26521 ttgtaaagcc ttatgtcttg cttgatacaa ttcctgttga tactatttat gccgataatt
    26581 caggtgtagt agtttttggt aaagatgctt ttaaaaacga aatcgaacaa tatatcccag
    26641 attctataaa gcaatcagtt tctgttgata gcttaaatca gataaaatcg cttgtgtatg
    26701 aacaattact aagcatgtat tcttctgaat tgcttaaaaa tacagggttt aataatcaag
    26761 aagaaattaa aaaaattgtt gatgcaactt ctcaaaattt atttttagtt gataaaagtg
    26821 gtattctaaa taaatatatt gttgcaggaa gtgttgatac tgtttttgtt caggaatttc
    26881 aacaagttca gaaggggcaa gctcttttta ctctttatca aattaaggat ataaaagaag
    26941 atatgcttaa tacaccattt aattttgctg agttttggta ttatttcaaa aaattcttaa
    27001 aagatttttg gtgggcttta cttgctttgc ttgtagttgt tgcaggttta atttacttct
    27061 ttaaatttta caaaaaagga gttaaaccaa catttttgaa aataaaacca gaagaaccgc
    27121 ctcatgtaat tgctctaagg gaattggaaa ttattagaaa agaaaaaata tggagcaaag
    27181 ggcaaataaa agaataccat gttcaaatta cagatgttgt tagagaatat atagaaaaaa
    27241 ggtttggtgt atttgctgtt gaaatgacaa caagtgagat tttagaaagt tttgaaaata
    27301 atgaattaat ttcagaagca gattttatga aattaagaca aattttagaa ttagctgata
    27361 atgtgaagtt tgctaaatat caagcattgc aaaacgaaaa tgatttgtct ttaaaaaaca
    27421 gcttcgattt tgttgaaaat acaaaagaaa ttattgacga aaactctaaa attattcaaa
    27481 ctgaggctgt aattgaaatt actcaagaag aaaatcacga accgacaaaa gacaataacg
    27541 atgatgaata atattgaatt tgcaaataag gaatattttt ggttgttttt gattttcata
    27601 ccaatgatag tttggtatgt gtggcgtctg aaaaagtcgg ttgctacaat gaaaatctca
    27661 agcctatcaa atctgaaaaa aaatccactt ccttggcgtt atattttacg tcattttttg
    27721 tttggattga gacttttagc tttatcagca ttgataattg ttttagctcg tcctcaaagt
    27781 atggataggt gggaaaatag aaaagtagaa ggaatagata ttgtaattgc ttttgacgtg
    27841 agtggctcta tgcttgccga agattttaaa ccaaatagaa ttgaagcagc taaagaaatt
    27901 ggaatggaat tcgtagcaag ccgacccgac gatagaatgg gattggtaat ttttgcaggc
    27961 gaaagttata ctcagtgccc tttaactata gatcatgcag ttttgttaaa tttattttcc
    28021 gaaattaaag atggaattat tgaagacgga acagcaatag gaatgggatt ggcaacagca
    28081 gtaaatagac taaaagaatc tgacgcaatt agtaaagtga taattttact tacagatggt
    28141 gtaaataata tgggaaacat ttctccaatg gctgctgccg aaattgctaa agaatttgga
    28201 attaaagttt acacaattgg aatagggaaa actggaatgg ctccttatcc ttttcaaaca
    28261 cctttcggaa ttcaatatca aaatatagaa gttaaaattg atgaggaagt tttacaaaat
    28321 attgctagtc aaacaggtgg taagtatttt agagcaacag ataatcaaaa acttagagat
    28381 atttataaag aaatagatag cctcgaaaaa actgaattgg aagtaaaaac ttattcgcaa
    28441 ccaagagaag agtttacttt gattttaatg atagcaatgc taatttttgt ggtagaacaa
    28501 ttattaggtt taacaattct taaaaatact ttttaatatg tttagatttc aaaattcaga
    28561 atatttgtgg cttttattac tattagtagt ttttatagct ttatttataa taggattatt
    28621 tattcgtaag caaaaactta aaacagctgg agatttaaaa cttattaatt tattaattcc
    28681 tgattttagt ctatcaagaa gaatatggaa aatttgttta atttctttag cttgggtttt
    28741 tatggttttg gctttagcac gtccacaatt tggtagtaaa ttagccgaag aaagcactaa
    28801 aggagtagaa attattattg cattagatgt ttcaaattct atgttggctc aagatttaag
    28861 accaaataga ttagaaagtg ctaagaaatt tatagaaaga ataatttcaa aattacaaaa
    28921 cgataaacta ggattaataa tttttgctgg tgacgctttt gtgcaaatgc ctattactga
    28981 cgatgttagg tcggcaagat tgtttttatc atcaatttct acagatttaa ttcctgtaca
    29041 aggaactgca attggtcaag ctattgattt ggcgtcaaaa tcatttaccg aaaatcaaga
    29101 tgtgtctaaa gtaattttat tgttgagtga tggcgaaaat catgaagacg atgctttagc
    29161 ggcaagtcag tttttaacag aaaatagaat taaagttttt actgttagcg tgggttctaa
    29221 agccggagca ccaattcctt tgaaaagagg acaaggtttt ttgaaagata gaaatggaaa
    29281 tgttgtagtt tctattcctg atgaaaaagt attagtagaa ttggcaaacg ctacagatgg
    29341 aatttatgtt aaattagata atactgcaaa tgcttcagat aaaattgttg acgaaatttc
    29401 aaaactacaa caaggcgaaa ttaaaaagca agtttattca gaatatgatg accagtttac
    29461 taaattcgca gctttggcat ttatatttct aattgtagaa gcggttattt ctgaacgtaa
    29521 aaatgatatt tttagaaaaa taaatttatt taaagataaa tctaacataa aaaagagcta
    29581 aataaaatga aatttttaaa aaatataatc aatataaaaa ctttaagtct gttgggatta
    29641 ttgacttttt gtatagtgaa tttgcaagct caggaagaaa gaaaaattat tagagaaggc
    29701 aataaattat ataaaaacaa agattatgaa aatgctgaat taaactataa aaaagcatta
    29761 gaaataaatc cagaattaga aaaactacat ggaaatgttt cagcatctca atatagaagt
    29821 ggaaagttta atgatgctgt aagttcttat caaaatgttt taaaactttt gggaaatgat
    29881 aaagacaagg cagatgcttt ttacaatatt ggaaattcat atctacaagc aggagaatac
    29941 gataaaagta ttcaggctta taaaaatgca atacgcttaa atcccgaaca cgaccaaagt
    30001 cgttataata tggcagtagc tactaaaatt caacaacaac aaaattctgg tggcgatggt
    30061 caaaatcaag accaaaatca agataagcaa aacgaccaaa ataaagataa caaggataac
    30121 aaagacaatc aagacaagca ggataagcaa gatgatagca agcaagacga aaaacaagac
    30181 caaaacaaag ataaagaaaa gcaaaaccaa cagcaagaca atcaaatgtc tcgagaagaa
    30241 atggaacgtt acttagaagc tctacaacaa caagagaaag atgtgcaaga taaggtaaat
    30301 aaagaaaaaa ttaaaacaca aaaacgagtg atagaaaaga attggtaaga actgattggt
    30361 ttgttgtaat cttaaaatcc atttatatta aaatataaag ttggtaacag taataaaaat
    30421 ccaagtacaa acataattaa aacaagcttc caaataaact taaaccacac gccataaggt
    30481 acttttgcaa ttcccaagca accaattaaa actcctgatg ctggtgtaat catatttgta
    30541 aagccatctc caaattgaaa agctaaaact gttgcttgtc gagaaattcc aataaggtcg
    30601 gaaaatggag ccataatagg catagtgagt gcagcctttg ccgaaccaga tggaataatt
    30661 agatttaaac aagtctgaat gctgtacata gaaaataaag aagtttcttt tccaaaactt
    30721 gccatcatat ttgataatcc atacatgatt gtatcaataa tttttccatc agttaaaatt
    30781 acaataattc caccagctaa acccacaaca aaagcagccg aaaaaatatc tttagctcct
    30841 tctataaaaa gtttagtaat atcatttgct gatttattca ttgccaatcc agcagctaag
    30901 cccatagcaa aaaataaagt tcctatttcc ataacatacc agtcgtagcc catcacacca
    30961 ataatcaaga aaattacagt aaaagccaaa aggtttaaaa tataaaaatg aaccgattta
    31021 cgtaatgcaa agaaagaact tatggcaaac aaggctgtag caaccggtag tgctgcaaaa
    31081 gtaaactctg catttccaac acttaattta gaaataggat agaaaacaga aaataaaatt
    31141 aaaacaatca gtaaaattcc aaaactaatc caagcactta aaggagtata atatgtcagt
    31201 tttgcatctt cattaacttt ttgcttacgc caataatcat caatatcata agtcaagctt
    31261 ttttcagggt gtttttttat ttttttagca taaagtaaaa taaatgtaaa acctacagca
    31321 tttataatca gccaagtaaa aactctgtac tcccaaccac tgaacaaagg taaatccgaa
    31381 agaccttgtg caatgcctat tgtaaatgga tttaaaatag ctccagcaaa acctatatga
    31441 gcagcaacat aaacaagtcc taaaccaaca atagaatcat aacccataga aatagctaaa
    31501 ggcactataa caataacaaa agctatggtt tcttcgctca ttccaaaaat agcaccaaaa
    31561 aggctaaaca ttgtcataat caaaatcata acaatattat ttacacctat ttttttaaga
    31621 aacttatatc gttcaagttt ttttgtggct ttaataaaag ataagattcc tgcatcaaga
    31681 gctttgctag catttaaaat ccaaaaagca ccaccaatta ataatataaa cacaataata
    31741 tgagcttgtg ctacaaaacc gttaaaaatc gatgaaaata tttgccaagt ttgaggttga
    31801 gaatcaacgt attcaaagga attttcaact atcatagtct ttgtagtacc gtcagccatg
    31861 agtttttgct ctcttttaaa ctcgcctgct ggaataatcc aagttaaaat agctgaaaaa
    31921 acaataatga aaaaaactat aacgtaagta tgtggaattt tctttagcat aataattttt
    31981 taatatttat acttgtttat tttgtaaacg ctacaaagct ataaaaaaag tacaaatatt
    32041 gaataaacta acttaatttt aatcaaaacc gaaggcagtt aaataacaca tcatgcaaaa
    32101 tataataatg atagcccaag ttgccaaaca accccatggc acataaaata acctacctat
    32161 gctacgagca tcttctttag taattgtaat tttacttttt gtgcgatagc gtttttcctt
    32221 gccattttct ttaattatat aaatccaagg gttctcgtaa agctcgatac ggtagcgttt
    32281 tattaaaaaa acaggaacta ttataaccac tatgcaaaac cacacggcag aaccaccaag
    32341 ttctaatctt tcaaaatcaa atccatttaa aaacaaagct attatcacag gtaagaagag
    32401 tataattaaa gctagcaaaa tgattcgtag tatccttaat ggtggagaaa atctcagttt
    32461 cacagcttgt ggtttaccat cgagttctaa gaaaaataca ggcggaacaa catttttctt
    32521 aatctctttt ttatctttga ttaattttcc atttattcgt ctaaatggac caccatatga
    32581 ggtgaaaatt ttgcgttcac cttttacctt gaacttattt ttaacggtat tactataagc
    32641 tacaagcata acaattaaca ttacaacact aaacatgccc gtaatttcaa ggctagagtc
    32701 aacatcccaa tcaaaataaa tgagagcacc aaataatatt gaagatgtta atattgtagc
    32761 tataatagct ttgagtcacc acggaataaa ataatgagtt tgtcttttta ttgacctaac
    32821 gcttactttt ccagtttgcc catttacggc agccatctcg ttgcctgagc atatataata
    32881 tacaggtagc aaaactggca tgcgaaatac agagctagta tcagtattga cctctattgc
    32941 ttttgtttct aaagtttttc gaacaacaga tgcgtagtcg ttacttactt cttgttctac
    33001 tcgctcaaca agaacatttt tatcaatatc accgactttt actctatgtc cagcgacata
    33061 ggaaaaatca aatgcttgta gttcatctaa ttcaaaaggc tccatgccgt ccaaaagcat
    33121 attgtcaagc tgtttggagc aattgataaa aacattatca acatatccac cgcaattata
    33181 tgaactatac gcatccatgc gactgacttt tgacttgaca ggacctttaa caaactcata
    33241 tggaagataa aatcctttta aaagattgat ttttttctca agattttttg cttctttttt
    33301 ttgcgagttc gctttgcacc agtcaagtaa gcagttgcgg gcttcttctt cagtgattct
    33361 aaaagggatt atgagttctg gcatttcttc agataagaga aaatcttttc taactagaga
    33421 acgtccacag aatgggcatt gggatagagc atcgccttct tcaaaaacaa tggtaccacc
    33481 acatccagaa cagttagcgc ttagtaaatg atgtttctca gcactttcct tgattttagc
    33541 ttgttgaata cttctaaatc cttgtttttg agcatgtgct tcattaatac ctaccttgct
    33601 attgcagtgc tacaaagata ctctcccttt attatataaa ttctgcagga actccgcaat
    33661 taggacaact tatatcagca aaattacttt tcatagctat ttattaaaca agttacttaa
    33721 aataaatggc ggaaaggaac taacaaggtt tcaactaatt tttgtaagcc atgtcttttt
    33781 ttccatttct catagttaaa tttatcacaa tctttttttg tgttttctat atgttcagtt
    33841 aagagtcgca aaatatctgg ttgtcttcca gcaatgttta tttcgtgcat atataaaaaa
    33901 ctgcgatagt caaagttgct tgagcctgta aaaaagtatt tgttatcaga caaaaatact
    33961 ttagcatgca agtttgatgg tctgtattct aaaatttcaa ctccattttt atgataagtt
    34021 ccaaaatatc tgtttcttaa aatatcaaaa agtgtaacat cagagttctt aggaacaatt
    34081 attgtaactt ttacgcctct tttactagca ttaattaagc ttcgttttaa taaagaacca
    34141 ggtaaaaaat atggagtttc aattacaact tctttttctg cagaatcaat tagttttcga
    34201 aacacgtatt ttactggctg cttccttgtt ccaggaacat cttgtactat agccatttca
    34261 tcacctttta gtatttttgt tccgtatttt ttattttgaa aaaatctact cgataaataa
    34321 tagtcataag aaaatatttt ctcaaacaca atacacaaca tgtcgctaac aattcgcaag
    34381 caacattctc gccagcttaa acaataagcg gtaatatttg cagaacctat gtaagtgatg
    34441 ttggagtcta ttagtaaaag tttgcggtga tttcgcctgt gattacgtga aaatacatca
    34501 aagctgactt ttattttttc aaaaaacttt aatttaccac ctaattcaac gaattcttta
    34561 aaaaaggatt gaccaacagc agttccccaa gagtcaatta aaaccttgac ttccacacct
    34621 cttctaacag cttcagttag tgcttgtcta aatctttgtc ctatgctatc gttgccaaaa
    34681 atgtaagttt gtaaataaac acttcgcttt gcttggttaa tgtcttcaag catagcgaga
    34741 tacaacttta aagtgtcgtc gtaaagtagg tatttttctt gactttgcat tttttaaagt
    34801 tttgacaaaa atacaaaact tagaaataaa taaaaaagtt atgtttataa aatgctaagt
    34861 caaacaaatt ggaattggtt tgcttatttt ttcttattaa aaaaatcttt agcagattct
    34921 ttgtaagtaa ctgtttcttt aaattggtaa gcacctgttt caggattttt taccatttta
    34981 atacattttg ctaaattttg tccgccacct gttctaaggg tagcaactgc tttctttgcc
    35041 ataacttaat ttatttaatt tctttatgta tcgtaacttt ctttaaaatt ggattatact
    35101 ttttaagctc cattctgtcg ggagtatttt ttctgttttt tacagttata tatcttgatg
    35161 ttcccggcat accgctttct ttatgctctg tgcattccaa aataacttga atgcggtttc
    35221 ctttagcttt ttttgacatt taaatttcct ccgactattt ttaattaata aatccttttt
    35281 ttctagcgtc tcttaaagct gcttgtaaac cttttttaga aatggtttta atgcctcttg
    35341 tggacacttt taatgttatc cattgttttg tttcttcatc ataaaagcgt tttgttgtaa
    35401 gatttacatc aaaaactctt tttgttctgc gttttgagtg agaaacattg ttcccaacca
    35461 tcgccctttt tccagtaatt tgacaaactc gtgccatagt aatcgtttct ttaaattttt
    35521 taggactgca aaagtcggca ttttttcttt tatatgcaaa ctttttatga acttttttta
    35581 taaataatga aatttagtgt tgtgagcctt gtaaaatcag caatttgata gtttaatata
    35641 taatgtgttt attttacatc tgtgttggtt tttattgcat tatactgaaa accaagtcgt
    35701 tttccagttt gcatttggtg ttttagtatt ttatcattca tataacttac gttaatgatt
    35761 ttaacatcat tatatggagg tactaataaa tcaaatttta aagcataagc aattgctgat
    35821 tctataagag ttctaaagtt ttctggattt acattattcc taccaaaaac aggtaaatta
    35881 aaaccaagtt ctcgtctgcc ttcaacaaaa aatgtatttt ctttattaat aaataacctt
    35941 ccaactaaat agccatagtc gtcaactcgg ttatatttta aagaatctga aagaaaattg
    36001 taaatattaa ttatccctga ataggtaata agaggttgat ctatataatg ttggtctttc
    36061 caaatttcgt gattacgagg aaactcaaaa atattagtgt gcatgttgaa gatgaggaca
    36121 tcacctgcaa tcttaatctc tgcatcaaaa tttcctctat cgcgaaattc taatctaacc
    36181 ctgtcgtcaa catcttctaa atcagtgttg tattctccaa caatttcttg taaaatgttt
    36241 ttaagcaaac caaatgcttc tagagttttg tcgtaaacct cttgtttgag cctagatgtt
    36301 tcttttaata ttgaaatgtt tttttctttt aatgttttat tttcttccat aatattttgt
    36361 ttttagtaag ctcttgcaaa tataactctg aatttagaag gtttgcctga gtaaatacat
    36421 ttcccttctt ctaattgtgg attaaatggt ataaatctta ttgtagcttt tgtttcttct
    36481 ttgatttttt cttcggtctc tcttgtgccg tcccagtgag cttcaataaa accaggagtt
    36541 tcttctaata ttcttttaaa ttcttcgtaa tcatctactc tgtaagtgtt gtcttcacgg
    36601 aattttaaag ctttttcata aatgcttttt tgaatatcct caagtaaaaa gtctatatgt
    36661 tctaataatt tgtcgttaga aataatttcc tttttaaaag tgtcgcgacg tgctacttcc
    36721 caagtgttgt tttcataatc ttttggtcca atcacaagtc tgataggtac accttttagt
    36781 tcccactctg caaatttgaa accaggacga tgcgaatctc tatcatcaaa tttataagaa
    36841 attcctttgg tttttaaagt tttgcataaa tcgtgtacgc gttccgaaat tttttctaat
    36901 tcttctttac ttttataaat tggtacaatt acaacttgaa ttggtgcaag ttttggaggt
    36961 aaaaccaaac cgttattgtc agaatgtgcc attactaaag ctcccatcaa acgagtagaa
    37021 acaccccacg aagtagccca aacatattct tctttgtttt cttggtttaa aaactttacg
    37081 tcaaatgctt ttgcaaaatt ttgagctaaa aaatgtgatg tgccagcttg taaggctttc
    37141 ccgtcttgca ttagagcttc gatgcagtat gtgtcgtcag caccagcaaa tctttcgctt
    37201 tctgtttttg ttcctatatg cacaggaata gcaagataat cgcgagcaaa ttcttcataa
    37261 acatgaatca ttttttcagc ttcttctata gcttcttctt ttgttgcatg agcagtatga
    37321 ccttcttgcc ataaaaattc tgcagtacgc aaaaataaac gagtacgcat ttcccaccta
    37381 acaacatttg cccattgatt tataagcaaa ggcaaatctc tgtatgaatt aatccaattt
    37441 ttataagtat tccaaatgat tgtttcggaa gtaggtctaa caattagttc ttcttctaat
    37501 tttgcattgg gatcaacttt aactcctccg ccttcagcat ttcttaacct atagtgcgta
    37561 acaacagcac attctttagc aaaaccatca acatgatttg cttctttgct aaaaaaagat
    37621 ttaggaataa aaagtggaaa atacgcattc acatgaccag tttctttaaa cattctgtct
    37681 aaagttgctt gcattttttc ccaaatgctg taaccataag gtttaataac catacaacct
    37741 ctaactgacg aattttcagc taagtctgct ttaatcacta aatcgttata ccactgtgag
    37801 taattttctt ctttatttgt aagtattttt gccatattaa ttggtatgaa atttgcatta
    37861 aaattagtaa aaaaagttct ttaaaaataa ttttacaaaa ctataaaaat tcatgatatt
    37921 atgaaagcaa ttttttacat attgattatc gccttgctag gaatgagttc ctgcacttta
    37981 aatttttaca caacaggcgg ttatgacgat gtttatctaa ctggttccga accaaaacaa
    38041 ggagctatca ataaacaaat cgttgatgag cataaagcta attacgaaga aacttataat
    38101 gcagagttta ataatactga tgattattac tacgatgaat attatgatga aaatggaaac
    38161 ttagtccaaa atgatgatgg taatgatact tataattatt atggtgatac ttataatttt
    38221 aatgattatg gctatgattt agatgatagc tattcatact actttaataa ttttagatat
    38281 tattatcctt catattatta cgacccattc tattatagtt actattggga ttggggctgg
    38341 ggctggggct atagacctta ttattgctct tattactact atccttatta ttactattgg
    38401 cacagaccat attattatta tggttggggt agtagttatt gggtcggata taatcatgga
    38461 tataccgacg gctactggga cggatactgg tggggaagta attctaatta catagtggac
    38521 aataacactt attacatcgg acatagaggc tctagtggtg gatatggcac aggcnnnnnn
    38581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaattat agacaaaaca ccaattgata
    38701 gtaagttaga agtaagtaaa gctgaaaata gtctatcttt aagtaatagt ggcaaaactc
    38761 cagcacaatc aggacgtaac gatgtggaac caaatactga agaaatgtct ggaaaaaaac
    38821 cagtcgctgt tgaaggtcgt attaataaca taggtgtaaa taaagacaat gagactggaa
    38881 agcaaccaat aggcgtgagt ggacgcgttg ataattcaag tttaagtaag ggtgaagtag
    38941 cagcaaaaca accaactgat gcgggcagac gtgttgataa ttcaagcaga agcgaaatcc
    39001 cagcaagaca accaatagga gtaagtagtc gtgctgataa tccaaactta aatagacaag
    39061 ttgaccctaa tgcaggaaaa actcaaggaa gctcttcaac acctgataat acaggacgcg
    39121 tttctactga taataaacct aaagaaccag ctacttataa taaaccagac ttaaattcaa
    39181 gaaataatta taatcaaaac actagcggtt ctcgtaataa tactgatgtg agacaacaac
    39241 cttctcgcaa tccttctaac gtaggacaac agccttcacg tcaacctgca agtagaggag
    39301 gagctacacc aagtcaaagt tattcacgcc catctaatac aaatacagga agttttaatt
    39361 caggaagtag tagtggacgc tcaagttcta gcagttctag tgcagtacgc tcaaatacta
    39421 actcaaatag atcttcttat tctactactc caagttctag tcgtagttca agtacatata
    39481 attcaagacc ttcttcaagt caaagaagtt ctagctcata taattcagga agctcatctt
    39541 cacgtagttc aggttcttca tattcaggaa gttcatcaag ctcgagaagc tcatcaagta
    39601 gctcttcaag tagttcaaga agtggaagct cttctagctc aggatttggt ggttctagct
    39661 ctggaggaag aagataaaca aattaaatta ttattttatg aaaaagtttt taatcataac
    39721 aattagtgtc ctaatatctt ggacaacatt attcgcccaa aacgaaatag atgctttgag
    39781 atattcatat ttaatccctg gtggaacagc tcgatataat gctatgggtg gttcttttgg
    39841 tgctttggga gcagaccttt ctgttatgag ctttaatcca gctgggattg gagtgtatcg
    39901 aagttctgaa cttacttttt caacaggatt tgttttaaca cagaattatg caacaataaa
    39961 tgataataaa gcttctgatt ttgattttaa ctttaatatt aataatatat catatttaag
    40021 ttctattaaa ctaaaacaaa ataatagcct aaaagcatta aactttggtt ttgcttacaa
    40081 tcgcttgaat aattttaatg aaaatattat aatagaaaac cctaaaagtg taaattctat
    40141 gacagattgg tttgctgcaa gaggaaacag aactttatgg tccgacttag gtgcaaaaga
    40201 ccaattttat agcaaattag cttgggaaac ttaccttatt gaccaaaatc taccagatac
    40261 tttgagctat atttctgcat ttaatggaaa atatggaatt gaacaaaaac agtctatcta
    40321 tcgttcagga aatcaaggtt cttacgactt ttctctatca gctaatattg aagataaact
    40381 ttttgtagga ggtagtttgg gaattacttc tgttaaattt aaagaactaa aacgtttaga
    40441 agaagtagat actcatgact ctataccaga atttaacggt ttcgcattta aagaacaact
    40501 tgaaacaagc ggtagtggaa taaattttaa atttggtgtt ttatattcgc ctgtaacatg
    40561 gatgagagtc ggagcagcaa ttcatactcc tactttctat aaactccgtg ataaatattc
    40621 aactcaagca gaatcagact ttttagatgc aagcatgtct ttaaccgaaa gctcaccttt
    40681 gggacgtttt gattatgaac tatcaacacc tttccgagct aatgctagct taggatttat
    40741 tttagcaagt aagtttttaa tcaatgtgga ttatgattat ttagattact ctaaagctaa
    40801 attgagaagt tttgattatg gattttatac cgaaaataaa aatatagaaa aagcatttga
    40861 tcaagcacac aacctaagac ttggattaga atataaaatt ggaggtgtgg ctttgagaac
    40921 aggtgtagct tattttgatt caccttttaa aacaggtcag gttaatcaaa atgcttatta
    40981 tatgacttat aatggaggta ttggtcttag aacaaactca atgtattttg atattgctta
    41041 ttcttacata aataatgaga taaattatta catgtatcaa ggacttggag ttgatagttc
    41101 tagaacatta cttaaactgc aaagacatag aattgtttta agtttaggtt tcaaattcta
    41161 aataatttaa caatataaga aattaagaga ctagttttgt ctcgcaaaaa ttatatgaat
    41221 gtatcgtaat tttgtgcgtt cgtatatttt taggttttaa tatacttaaa cttaaaacct
    41281 tttttgttaa aatccgtatt gactacgaca aaataataaa ttttatacta attattagat
    41341 atatttcaaa ataaaaatga gacaattaaa aattacaaaa tcaattacca atagagaaag
    41401 tgcttcttta gacaaatatt tacaagaaat aggaagagaa gaacttattt ctgttgacga
    41461 agaagtggaa cttgctcaac gtattaagca aggtgatagg gaagcattag aaaaacttac
    41521 tcgtgcaaat ctgcgttttg ttgtttcagt tgcaaagcaa tatcaaaacc aaggtttaag
    41581 tttacctgac ttaataaatg aaggaaactt aggtttgatg cgtgctgcag aaaaatttga
    41641 cgaaacacga ggttttaaat ttatttctta tgcagtgtgg tggatacgtc aatcaatttt
    41701 gcaagcttta gcagaacagt cacgaatagt gagattacca ttaaaccaag ttggttcttt
    41761 gaataaaatc aataaagcat tttcaaaatt tgaacaagaa aatgaaagaa aaccgactac
    41821 tgaagaattg gcaaaagctc ttgatttacc tgaatataaa attaatgata cattaagagt
    41881 ttcgggtcat catatttctg tagatgcacc atttgtacaa ggcgaagaaa attcattgtt
    41941 ggacgttctt gaaaatacag atttacctaa taccgatgca cagctgataa aagagtcttt
    42001 agctaaagag atagaaagag ctttagcaac tttaactgaa agagaaagag atattgtgaa
    42061 attgtttttt ggtatttctt gtcaagaaat gacattagaa gaaattggcg accaattcgg
    42121 cttgactaga gaaagagttc gtcaaattaa agaaaaagct atcagaagat taagacattc
    42181 ttcacgaagc aaattactga aatcttattt aggataaatt ttagataaat aaaatacaaa
    42241 aaactgttgt ctcaaataaa ttttgggata acagtttttt attttaaaaa taatttcaaa
    42301 ccaaacccaa gtttgctaag tttaaatcta attgtaagag atttaaaaaa tcctgctttt
    42361 ttaatactta cattattaat gtcagcaagt ttgacaaata aagatttttt taaaaaagta
    42421 tgtttatcaa ttctgtttcg tctccactct tcgtttaccc aatgaatgac ataccattct
    42481 tgaggaattt caatattttt gcttagaaat ttgcgaatta aattccaaga atcctgattt
    42541 gaaaaatttc tgatatagct tgttaaacca agttctttaa cattatcaac taatatttga
    42601 attggaaggt gccaatctct attgttttca tctacttttt ctacagcttc ttcaaagcac
    42661 tttttcatca atagactgtt ttttggacat ttcataatgt ttccaacaag ttctaaatca
    42721 tggtgagttc tgaaaacata gtcagtttca aaatctaagg gtttcaaaca agttacatcc
    42781 atatctgtcc accaaccgcc atgtaaatat aaaagtttat atctaaaaat atcactaaat
    42841 ccagcaaatg aacctttgcc atgtccaaat tgattagaat atttataaga aaaaacttta
    42901 gattttggaa aaatttcatt ggcatctttt aaaataacgc ctttgggtaa ttcagttttt
    42961 atttcatcat aagcccataa tacaaaacta tgcccattgt ttaaaaatga cttaatagtt
    43021 aataattcga ctgaagataa atgctcgcca atccataagg cgttaacaat tttgttgcca
    43081 tcatccattg ttcaaaatct tttgaataac aaaattaata aatttttttt aaaagcttta
    43141 ttttttatca gaaaaatgca attaaatata atttttactt tgatttcatt atatttgtag
    43201 tttgaatttt aaacttatga gacaaatata cgactttgca aaatattact atcagaaact
    43261 tagtcgctta aaattaccat ttatgcgata tcctgatagt aaaactaaaa attttaagtc
    43321 gattcctaga agcaaaatga aagagtttaa taaagttcgt tttcatggac caaaaaagct
    43381 tgcttgttac aatccttttg taaatttgtt ttttaatagc cgtggacaag ctgttgtctg
    43441 ttgcagaaat caagatatag tattgggtac ataccccgaa aacacaatta gtgaaatgtg
    43501 gtttggaaat gttgcaaata aacttagaga gcatatgtta aataacgatt tgtcaatggg
    43561 ttgtggatat tgcgaacatc agtttaagac ttctcgtttt tatgggttgc cttctatgca
    43621 tgcagattat tattcaagca caaaacttac atatccaaaa atcatagaac tagaattgtc
    43681 aaatatttgt aatttacaat gcgttatgtg ttcggggata gtttcctctt cgattagaaa
    43741 atatcgcgaa aaacaagaac ctttaaaaat tgtttatgac gataagttta ttgaacagtt
    43801 gaaagagttt ttgccttatg ctaaagaaat taaattttat ggtggagaat catttttaat
    43861 tgacaattat tataaaattt gggattttct gttagaaata aaatctaagg caaaactaca
    43921 cgtagttact aatggaacaa ctttaaatga caaagtaaaa acttatttaa aacatttaaa
    43981 ttttacaatc acagtatctt tcgatgcaat gaaaaaagat ttgttcgaat ctatacgtgt
    44041 tggagcaaat tttgaaaaag taaagtctaa tattttagaa tataataaaa ttttaggcgg
    44101 aaaaggtttg tcattgagca ttacaccaat gaagataaat tgtaaggaag tccctgaaat
    44161 tattgatttt tgtaatgatt tgaatgccac agtaaatatg tcatatgttg aaaatcctgc
    44221 agaaatggcg ttatggacaa tgccttcaaa acaattggaa gagttgatag agttttataa
    44281 attttataaa tttaaaccta ttaaatctgt aaatgcagac tataatttta aagcgttttc
    44341 tcaatttgtg aatcaaattt ctcaatatca aaaagttaat gcaaaaatcg aaaaagaata
    44401 tgaacttact ttgaaaacaa aagacgagtg ctatcaaatt ataagtcaga ctttagattt
    44461 tgttttaaga gaaaatataa ttttcgaatc tgataaaaca gaaatttata aagttataca
    44521 agaagttgaa acggaattgt cagaagatga caagaagttt ttttatggta atttgacaaa
    44581 ttttttagaa acaaaaggtg tccctgcttt aaaaaatttt gtagcaaatg gttttgatgt
    44641 aaaaaagctt aaagatgttt taagtagtct ttcggaaaga ccaaatattt atatcaaaaa
    44701 ttataaaaat attagtttgt aattttttat taaaatgagt tctcgcagaa tagttcatgt
    44761 tgatttagat agcttttatg tttcagtaga gaggctactt aattctcgct tgaatggact
    44821 tccaataatt gtaggcggaa attcagatag gggtgttgtt tcatcttgtt cgtacgaagc
    44881 aagggctttt ggtgttcatg cagctatgcc aataagaaaa gcaaaacaat tatgtccaga
    44941 tgcagttttt atcactggaa atatggaaaa atactccaag tattctaatt tagtaacaga
    45001 tattattgca gaaaattcgc ctgtttacga aaaagctagt attgatgaac actacataga
    45061 tataagtggt atggataaat tttattcatc acttaaatgg gcacaagatt taaaaagcaa
    45121 aattgataaa aatactggct taccaataag tttcgcagta tctgttaata aaaccgttgc
    45181 aaaaattggt acaggtgaag ttaaaccact tggagaaata tgtattgaac aagcagaggt
    45241 aaataatttt ttggatccgt tagcaataac taaaattcca atgttaggaa aaaagacatt
    45301 cgaaatttta agtggaatag gcttaaaaac tataaaagat attagagaaa cacctgttga
    45361 aaagattgtt aaattgttgg gaaagaatgg tcaatcaatt tgggaaaaag caaatggaat
    45421 agataatagc gaagtaattc catatcaaga acaaaaatcg atgagtagtg aaaccacttt
    45481 tgctaacgac atcaatgata ttaacaaaat gcttgatatt atttctgaaa tggctgtgaa
    45541 tttagcttat aaactacgca aagaagaatt attaacctca acagttactg taaaaatacg
    45601 atattcagat tttgacactc aaacacttca aaaacaaatt ccttattcag ctttcgacca
    45661 tcacttaata tcacatgcta aagaactttt cttaaaactt tataagcgaa gagcaaaagt
    45721 aagattaata ggtgtgaaat tttctcaatt agttcgagga gcaacacagc ttaatctttt
    45781 tgaagatgct acagaaatga ctaatcttta caaggcttta gataaaatta aaaataaata
    45841 tggcgagcaa ataattaaaa gaggaaattc tctgtaagta ttcaaaaact attgctaaaa
    45901 tcactttaat taagtttttg ataagttttt attgaaactt tcaaaaaata attgttaaag
    45961 aatgcaaaac aattgttaaa aaaaataaaa atatttctac tatttaacac aaccttttta
    46021 aaaataatac gtaataaatt atagagaagt aagtgtttca tttgttaggc aaagttcttt
    46081 gatttcggat tttatcaact tttaaacatc gactttagtg ttttatcgga atgtttaacc
    46141 tttaaaaaat tatgattata aaaacaatta ttgttacttt aggctttttg tttgttgctt
    46201 cgttaggctt tgctcaaact cagcttagca atgattttaa caatgcaaca gaaatgtatg
    46261 ttaatactga atgcattttt gaaacttata ggtataatca tgattttggt tctgatctac
    46321 cagacttaaa atgtgataga atgtatccag gtgttagttc ttactttata tttaaaatca
    46381 cagaaaattc aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    46441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaaattca
    46501 atttgaaaca gaaaagttct tcggtttagc tttttatact ttgcaaaatg gagaatatgt
    46561 agagttaaaa tgtgatactt ataaatcgac agaaggagag ttgaagattt attgggacga
    46621 aggattggca aatgttgaca ttatagcacg cttttggatt atgggtgaat taggctcagg
    46681 tgaggtggat atttgtttgt catcggaacg ccagaattta caaccaaaag ttttaaatgt
    46741 tagcacaact caatatactg ttcaagaact tgttcaggaa gtgttaatta ctggctgttt
    46801 aacggcttct aatattactt ttactggaaa ttctgctcaa ataggctatt tttctaatgc
    46861 aataccaggt cttgattttt ctagtggagt aattttaagt tcaggtagag taactgatgc
    46921 tccaggtcca aatacatcag gttcaacttc tacatctttt ggaggagcag gcgatcctca
    46981 actaaactct ttaataccag gctatactac tcgagatgca gcagttttgg agtttgattt
    47041 tgtacctgct tctgacatgc ttgagtttca atatgttttt gcaagtgaag aatatcctga
    47101 atatgctcca ccaaacactt caacatataa tgatgtattt ggttttttca ttagtggagg
    47161 tcctgaaaat tataataatg ttaatgtagc tcttattcca ggtacagtaa caccagtaag
    47221 tataaataat gttaatgctg ttacaaattc gcaatattat attaataatc aaggaggggc
    47281 tgatatggaa tatgatggtt tcactgttac gttcaccgct tccaagccag taacagcatg
    47341 tgctacatat catattaaat tagcagtagc tgatgttggt gatatgatct atgattcatc
    47401 tgtgtttttg aaagaaaact cttttgcttc gggagagtct tatgttgttg aaagttataa
    47461 tgcttggtcg ccaagtttaa gtgtaatgag aggttgtact aattatgtta agttctgtag
    47521 aacaacagca attcctttaa gtcaaccagt acctgttgaa ctgtttattt cgggtactgc
    47581 agtaatgggt gtagattatt ctgcaatacc tacaaatttt caaattccag cagggcaaga
    47641 atgtgtaata attgaatttg atgcttttga tacaggagtt cttggtgatg ctacaatagt
    47701 tttaagtttt gatagtggtt gtccttgtga tattgagatg gctgaacatg ttataacact
    47761 agttgaccca tatccagttg aagctactat aataggtagt gctccaattt gtgttgggga
    47821 aacaacaagt ttatctgttg atataactac aaatgatatg acttatgttg atattgtgtg
    47881 gagtacagga gttgaagatg taactacaat aaatgtatct ccaacttcaa caacaactta
    47941 tacttgtaat gttatttatc cttgcgatac tattacttta gaaacaacag tggttgtagc
    48001 tcaacttcct gtagttgatt taggttcaga tttttctgtt gaggctgtaa cagctaatct
    48061 taatgctaac atgacccctg gaaatacagg ttattggact gttatttctg gtcctggaaa
    48121 tgcaactatt tcaccaataa atagtccaaa tgcaacagtt acagttgata catatggtac
    48181 ttattccttt tcttggacag aaactagttt agcaccaaac tgtgttgatt cagatataat
    48241 aaatgttact tttttccatg ttcctattgc agatcttgag gcttctccaa tattgtgttt
    48301 tggcgataat accataatta cttttactgg tgaggcaatt cctggttttg ctactttcaa
    48361 ctgggatttt ggaagtggaa ctatagttga tggaacaggt caaggtcctt atattgtgaa
    48421 ttttggaact ccaggaagtc attcagtaag tgttactgta acagaggctg gcatgacttc
    48481 aagcaatagt gtaaatgttt atgtaccata tccaattagt gcaacgctaa caactaatga
    48541 tgcatcttgt tatcaaactt gcgatggctc agcaagcgtt tctgttacag gaggcacgcc
    48601 accttatact tattcttggg gggctaatgt tattaacaat ttgtgtgcag gtaactatgg
    48661 tttaacaata acagacaata atgcttgtga gtacataact tcattcacaa ttgatgaacc
    48721 ttcagcttta acaagtagtg ttacagttca aactaatcaa atttgttcta cacctggttc
    48781 t
//
LOCUS       DAOPNX010000008        45667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_1046_length_45667_cov_7.060927, whole genome shotgun sequence.
ACCESSION   DAOPNX010000008 DAOPNX010000000
VERSION     DAOPNX010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 45667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45667
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1046_length_45667_cov_7.060927"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..774)
                     /locus_tag="PKN53_01650"
     CDS             complement(61..774)
                     /locus_tag="PKN53_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOD87691.1"
                     /translation="MMKCIIVDDEPIAREGMKMNIGELSNLTLVGEFEDAASANEFLK
                     NNQVDIMFLDIEMPGQSGLDFLRSLDDAPMVILTTAYPQYAVEAYELDVVDYLVKPIK
                     LDRFIRAVAKAETFLSLAENTAVFEAVEDDFIFIKSERKYVKIRFDELLFVEGLKDYV
                     IVHATHGKYMTAMNVKTIHNKLPEDMFFRVSKSFIINVNHIDDIDGNVINIRENEIPI
                     GRSYRDVFLDFVNKRLLRR"
     gene            complement(771..1790)
                     /locus_tag="PKN53_01655"
     CDS             complement(771..1790)
                     /locus_tag="PKN53_01655"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOD87692.1"
                     /translation="MQKKSNYNFLIAFVNVVFWLFYFLSALSLYFSGLISVPFEGFLS
                     IVLIDLLSITFLVYYYVDKVKLLEKTYYKIFHAYLAVLFVNFGINFLLQNFIFGDFQS
                     SFSTCLIKQSYSFILSFVFISFAFIMKTLLTYRDKLETITHAQNAKISTELDELKNQI
                     NPHFLFNVFNNIYIQIQVDPQKAREMLLKFSDILRYQLYDCVEGKVFVKSEVEFLQNY
                     IELQKMRIANIDIKFEQKGNFSGLMVYPFMFLPFIELAFKKVGNVSNNEKFIDIYIAV
                     VEKNIIFTVKNSENAISSNFDLENDENFIKIKNRLEFFYKHNYELKMKNFDNYFIVEL
                     KLYIK"
     gene            complement(1946..3622)
                     /locus_tag="PKN53_01660"
     CDS             complement(1946..3622)
                     /locus_tag="PKN53_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87693.1"
                     /translation="MKLIFRYSLLFIVFCVFIQKINFAQQLNILTINSENSEQISLKT
                     QNFTDSVDAENKLNQFLLSEFSKGFYSAGYDSIVYAEKLVNAYYNSGQKYILKELDFE
                     IDSLAKTLNFKDFDKEAFFDPSFNHEKYLKVLADKGYPFAKFEIENIEIENDSISAII
                     KLDSGEYFVFDSIIIKSEAKIKPYYINKVLNIKKNDAFSYSKIKNINKSISGIPFLQQ
                     TRAFQLAFGENKSDILLYLKNKKAGSFSGVLGILPNNITTGKLLLTGDLQLNLVNSFA
                     RGEVLSVNWKKYDAFSQNLETDFLYPYIFKSDFGIGLGFNLEKKDSTWLNTLFDAQII
                     YGNSNATGFNLFLENKNSIVLSSATDGLSSVKSKLFGFGFRLINIDNILTPNKGIVLF
                     LNSGFGLKNEDFIEEDKKNIFQTKNKLELTAYLPIYRNLIFKFKSNTSSIFSKKLFEN
                     ELDRIGGLKTIRGFDDLSLPASSYSIASTEILYRIEENSAVFALCDFAYFEKRNTINK
                     EYNYAVSFGAGIELNTGAGIFSLVWAVGKLNSNSFMFNASKIHFGYRNMI"
     gene            complement(3619..4767)
                     /locus_tag="PKN53_01665"
     CDS             complement(3619..4767)
                     /locus_tag="PKN53_01665"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HOD87694.1"
                     /translation="MKKLVFLIVFFGFVNASFAQIKVYSISNEELKVKENMYVYSLPK
                     VVFQVKVNVATECFYPGPFAQYAEKYLIISDVKTEYSEFSDISNIEIIPKYITDFDAS
                     FVVQSKKEPKIYLNENGVISSYGVQIDSDDNLLLDSDLLSESFEFSNFHFPLFTDLTV
                     EKNFSNITDTTYKVVQIDSIYQKIPVYNTSITSKTFEQKAEEAANFILKIREEKFFLL
                     AGMFEWENPPTELEFMVAKLEELEKEYLSLFIGKVEYKYQEYDFEIIPDGNVETQKVK
                     LLNLHAEEPVQVYFELTKNQKHSKISNFYKNKANSTKANKPQGLYYRIPGNATIKVKV
                     ENVDYYKANVVVPQFGQIEYLPIKMFKNKKLKIIFNENYGSIQSIGNE"
     gene            complement(4828..6525)
                     /locus_tag="PKN53_01670"
     CDS             complement(4828..6525)
                     /locus_tag="PKN53_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOD87695.1"
                     /translation="MKVFTSEQIKKIEEYTIKNRPIASIDLMEEAAAELLDPIVECLR
                     KKDDTIDIRETRFYFVCGPGNNGGDGLALSRMYKALDFDVLTVFCQFSSKISPECKTN
                     LNKLKRNKNHNLHIITDVEQLPDFEDRVIIDCIFGNGLNREVLGKYAEVIEKINESQN
                     TVVSVDIPSGLFGEDNSENNGAKVRADFTLNIGYLPLSSLFAENEKYFGETICVKIGF
                     LEEIEQKLETPFYVISKNLPQDMLVDRKRFAHKGDFGHALLIAGSYGKGGAAILAAKA
                     CMRMGVGLLTVHVPEKLVDVLQISIPEAMLSIDENEKYFSQIEDFEKYNVIGIGPGLG
                     LNDITKNAFANFLKTNTKPLVIDADGLNIISENKELLKYLKPGTILTPHPKEFERLFG
                     KFDSEFKAIEFMRDFSKKTGIIIVRKSGVTAISTPEAKVYFNTIGNPGMATAGSGDVL
                     TGMITSLLAQNYSAQNAALLAVYIHANAGDFASFVKSQETMIASDIIDHLGNSFNYLR
                     NVNSPLSELFDEAHNMPDQHDEFFDDYWADDDEDDYWTDDEDDDYWTDDEDDEVYLN"
     gene            6667..8259
                     /locus_tag="PKN53_01675"
     CDS             6667..8259
                     /locus_tag="PKN53_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HOD87696.1"
                     /translation="MFRNHPKGLLGASLANMGERFGFYTMMAILVLFLQAKFGLNGKD
                     AGLIYSIFYFSIYILAFVGGLIADRLRNYKGVIFVGLIVMATGYLMIGIPTETPVANQ
                     LTMLVITCVGLFVIAFGNGLFKGNLQALVGQMYDNEKYGKMRDSGFSLFYMFINVGGF
                     FAPFTAIGIRNWWLERHGYAYDANLPALCHQHIDNTLEATKVVGTGALDKAQGVAEKV
                     FSLDPISKFQNLASEVGGQTENLTNFANDYLNIFTSGFHYAFIAAIGAMIISLTIYLF
                     NKKHFPNVDKKAKATESQDSEIKMDAKEVKQRLYALLAVFGVVIFFWFSFHQNGLTLT
                     MFARDYTDLSGIKINLGFVTIQGAEIFQSINPFFIVFLTPIIMAIFGWLRARGKEPST
                     PKKIAIGMGIAAIAYVVMAIGSYDLPLFKDIQSSPLDNALKVTPFLLIGTYFILTVAE
                     LFISPLGISFVSKVAPPHLQGLMQGGWLGATAIGNQLLFIGAILYESTPLWLTWTVFV
                     VACLLSMITMLAMLKWLERITK"
     gene            8280..8771
                     /locus_tag="PKN53_01680"
     CDS             8280..8771
                     /locus_tag="PKN53_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87697.1"
                     /translation="MKKIIYFFVIVALSLTTIACSKQNAVEDVERSIAKLNQFNSTFE
                     KFYNDGVIDGINTKGDKNKDEDSEYNQLKKLAEEYYELINRINSNINEDKENVKKGKK
                     SNGYEDEYKKAVESKKDEIEKATDLFIKNLSILNNCSEDYKIIEEETQDVITEETVEE
                     TVE"
     gene            8873..11554
                     /locus_tag="PKN53_01685"
     CDS             8873..11554
                     /locus_tag="PKN53_01685"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOD87698.1"
                     /translation="MKQIFKFIGLSLALMLFSIVIFAQQDCSNPHMITTIPFSATGLS
                     TEGMGNLFDADDLCTSNAMVNEEYIFQFTPAEDMQINIALENTEVTYSAFPMATDDAN
                     IGLFLTNKCPDEPAGTCIASVDNQTSNPSLNNINVSAGQNYFIIVSSANVPFLGGTNV
                     SFDIKITRIVDYDLEVTSISLPNSSCSLGDEQISCVIKNNGTSNVTSPFNISYSVNGG
                     TSVVESCSDPINSNSEITYTFTNLVSFTNTGEYNIEVTIDFPLDENVNNNSLSATTYY
                     LPTYTSFPFTEDFEAGKGFWSTGGTSSSWAYGNPSASPEDLVINSAASGQFCWITNLN
                     GNANTNENSYIESPCYDLSALLLPCLEVNYWGDFAIYGNSASIIASADNGATWDTIAN
                     LNASENWETLIIALPNLVNETNVKFRVTFKSSFLVANGLAIDDFTIKESVLKDVGVSN
                     IISPNTGCGLSDIETVIIEVTNHGIQAQDNIPVNYSIDGGVTWLASDEIIPSIVSEGT
                     VQHTFTITGDFSEIGEYEIIAKTNLTGDEVADNDSFTKTVYKQNTIVVTDTYSESFET
                     GNAGWLAYGTNSSLELAQPNGTIINQAGDGEYAWVTNPNGPHNTNETSYLESPCFDFS
                     TLTNPIFKTMIQYETQMIMSNFYVEYSTDGIAWDTVRAGTVSNNWYGSGFMSFGTWGG
                     ASEGWLDASTEIGFLAGQSSVKFRFVFNSSSFPWGGENTEGVAIDFIRIEECADIPEA
                     MFSFETNQLQVQFTDESTNANTWEWSFGDNAFIPSTSTEQNPSFTYSQDGQYTVSLTV
                     TNACGVSTYETTVNVTTTNLNENYKDLTNIYPNPTSNFINIENIQNSEINIYDVNGKL
                     VYSKFIKENKLVLDIKDWANGIYFIKTPTTSQSFIKE"
     gene            complement(11791..12231)
                     /locus_tag="PKN53_01690"
     CDS             complement(11791..12231)
                     /locus_tag="PKN53_01690"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOD87699.1"
                     /translation="MTDLSSRIKAVSASPMKTIVKARNFEIIIDEPQEMGGTDEGASP
                     VEYLIAAFSGCINVVAHLVAKEMNFELRGVEIDIYGDLNPAKLFGQSNEERAGFKQIN
                     LSIKADTDADEATLEKWLEAIESRCPISDNLQNPTPVKISYKKK"
     gene            12398..13057
                     /locus_tag="PKN53_01695"
     CDS             12398..13057
                     /locus_tag="PKN53_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOD87700.1"
                     /translation="MQEIKRFSWATNLTEEEYSELIKSSSLISYNKNETIVKQGSLAS
                     QILMLEEGFVKLNIEIKGKDSTFRFSGDGDFIGLACSFVKKKLDFSAVAVTTSKVRVF
                     DRNIFEDLIQKNGKFAIDLITIMSEMTSAAIHSLIMISHRNANGALAILLLRLYTLFK
                     SEKFTLPFTREEMAISLAYSKESVINILSDFQKDGIIKLSGRNIEILKLDSLKIIAEN
                     G"
     gene            13085..14497
                     /locus_tag="PKN53_01700"
                     /pseudo
     CDS             13085..14497
                     /locus_tag="PKN53_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304575.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(14616..16697)
                     /locus_tag="PKN53_01705"
     CDS             complement(14616..16697)
                     /locus_tag="PKN53_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87701.1"
                     /translation="MQIYFEYPKYLILVAVALAAATSFLLYYKDKLYKELAKWKKILM
                     ASLRFVFVLIILLLLLNPIFKSSETIVQKPILAVVQDNSASILLNKDSVYYKNQYKTE
                     LNKLLADLKNRFDLRYLQFDEAINTDTVLNFKGQLTNISSIFPEIISSYAGMNLGAVL
                     IATDGIFNSGENPVYLNNINFPVYSIALGDTISQKDLMLKDIMHNHIAFLGNKFPVRI
                     LISAEKCLEKESEIIVTKDEKIIYKEKFELPENSKQKYIDIELFADKVGTQQYSVELK
                     KLDSEISYENNFADFIINIVDNRNKILLLANSPHPDLGAIQYALKNNPDFELDLRYIY
                     DFNESVKNYDLVILHQLPSVQFAATTILSEIKKNSIPSLYIIGANTSIQAFNSVETGM
                     KIVANSNKMDDALGSLNLNFVAFELGVDQSFFKYLSPLKVNFADYNFENEAKILLYQS
                     INGVKTTKPLIAFVDDNLGSKLGVICGEGIWRWRLDDYKYNSDHESFNAFINKTVQYL
                     LVKRIKDKLNVEFKQIYNENEPVLINASFYNEAFDLVPNLDLSLVLTNEEGKEFTSSF
                     LNFGNSYRLDMGRLEAGKYKFNVETKFDNRIYEFAGQFLVKSINIESLITTANHSVLH
                     QLAEKTNAKVFYPNEMNKIREQIEADQNIADVAYKNEKLHDFTDLYLLFFVILLFAAV
                     EWGLRKFWGSY"
     gene            complement(16705..17451)
                     /gene="fabG"
                     /locus_tag="PKN53_01710"
     CDS             complement(16705..17451)
                     /gene="fabG"
                     /locus_tag="PKN53_01710"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOD87702.1"
                     /translation="MKLLDGKVVLITGAARGIGRAIALACGKAGANVAFTDMNADDNF
                     KSLEKELTSFGVKAKGYVSNAADFNSTEKTVNEVVADFERIDVLVNNAGITRDALLMR
                     MSEEQWDLVINVNLKSVYNLTKTVQKTMLKQRSGSIINMSSVVGVSGNAGQANYSASK
                     AGLIGFTKSIAKELGPRNIRVNAIAPGFIETEMTKAIPEEARQAWAEKIPLRRAGQPE
                     DIANLVLFLGSDMSSYISGQVINVCGAMLT"
     gene            complement(17564..18439)
                     /locus_tag="PKN53_01715"
     CDS             complement(17564..18439)
                     /locus_tag="PKN53_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOD87703.1"
                     /translation="MELFQTYSIGDLELISEVKASTIRMWEKRYELFSPLRSVQNERI
                     YSQKDLNLLLKITVLQSFKFKISYLSSLSEAEFENLFEEYSLKFNSSNKYLPEFLFFF
                     LEKDGEGFENLFDKVYEQLFPEEFIVNVLEPLIFKIKKLIQIRNIDSIYADYFFNKLT
                     LKILCLAEKEKTFRSSKEILIFQSDSSAFPCNLALVYFLATVKKYKIHFYFNKLTIES
                     LEKMKGVFQPDVVYTEFNENITSAKMLKYFEVLEEAFPMSKNIIGGSRLKDGWKLLLD
                     KIYTVKNLESLSKIL"
     gene            complement(18575..19996)
                     /locus_tag="PKN53_01720"
     CDS             complement(18575..19996)
                     /locus_tag="PKN53_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87704.1"
                     /translation="MHYHKIVFFSSLKNFSIKLILVKLIQMVCIVYLVCSCSNINKKE
                     EEFKISYNLDEYISIYNAGRSPIEFKVLPNNKDFSSLDNIVSYIKDKAKEFPEKPLEI
                     CAWEFVYNNTFHDKDIVIDDWTRTTMPELILSSVGGGLCGTRSAVLTNILVALGYKAR
                     SWCIEGHVVTEVFSDGKWKVLDPDLGVYFMNDDKQIASYKELCDNPQWFNDTARYVVI
                     DSPKMTFTMAFVMNNALLFSTLEDNRVFYTNFDYKLENENIYFLPEGAELIIPWKNSE
                     RGNFFLCAKLIMPSNYKGKIKIPLVLDKIKGKGKIKFNKKRYEIDGCMTNFGDFNLML
                     GNFSIIENHGMEFYFFINPQLFFEKEINEIVVKSRDINELEIKKEKRKEDEILLLPQC
                     KKLLTNNIYNMLNEYSQNHNLEIFDSSDKTIEYLCDTCCNLYFSKPIQLIDTLKDLDI
                     MEQKYFFNQLEYFIQNKCDCKNE"
     gene            complement(20479..20724)
                     /gene="rpmA"
                     /locus_tag="PKN53_01725"
                     /pseudo
     CDS             complement(20479..20724)
                     /gene="rpmA"
                     /locus_tag="PKN53_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381900.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
     gene            complement(20744..21055)
                     /gene="rplU"
                     /locus_tag="PKN53_01730"
     CDS             complement(20744..21055)
                     /gene="rplU"
                     /locus_tag="PKN53_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOD87705.1"
                     /translation="MYAIVEIAGQQFKVEKGNKFFVHRLENEEGSEVTFDKVLLLDDN
                     NKVTVGAPVVEKAQVSAKVLRHCKSDKVLVFKKKRRKGYQTLNGHRQYLTEIEIQDIK
                     A"
     gene            21290..22033
                     /locus_tag="PKN53_01735"
     CDS             21290..22033
                     /locus_tag="PKN53_01735"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOD87706.1"
                     /translation="MRNFIFYISVLSFLLPINLSLAQKPIPMNLPKLDQQVAHFGYSL
                     GLNTMDFNIRPSANFLSEFDTVMAVEVERFVGFNINIISNLRLAEHLDLRFLPGLVFG
                     QRNLEYKFIKNNQFRRHTMMIESTFVDLPICLKYRAARINNWRPYVLAGSSVRIDLAS
                     QRKINPEERPKIRLKPFDTYYEVGVGLDFFLEYFMFGIELKSSWGIFNIVAYDKTQYS
                     QYYKKLNSHMVILGFHFEGGKIDKIKWWK"
     gene            complement(22335..22955)
                     /locus_tag="PKN53_01740"
     CDS             complement(22335..22955)
                     /locus_tag="PKN53_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOD87707.1"
                     /translation="MIIKNIIFDLGNVILNIDINKSEIEFKKHGLENFDQLYSLVSQT
                     KIFDYLETGKISPQEFYSQLRQISNTDLDDKIIETCWNALILDFPPKRIELLLSLKNK
                     YRIFLLSNTNAIHYEYYNANFVKEFGFASFDEIFERAYYSHTIGFRKPDLQAFEFVLN
                     DSKLKASETLFIDDNKANIEAAKQLGINTIWLPDNNLETLEILQTL"
     gene            complement(22969..23382)
                     /locus_tag="PKN53_01745"
     CDS             complement(22969..23382)
                     /locus_tag="PKN53_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008822334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87708.1"
                     /translation="MKIAVDFDGTIVEHKYPDIGNVIPGSFEVLRELQAQGHKLILWT
                     VRDGIDLQNAVNFCLENGVIFWAINESYPNEEFNKYISRKVDVDIFIDDKNIGGFLGW
                     QKIREILIPDSPHIVKLEEPKVKKRTSFWKRKSKK"
     gene            complement(23448..23876)
                     /locus_tag="PKN53_01750"
     CDS             complement(23448..23876)
                     /locus_tag="PKN53_01750"
                     /inference="COORDINATES: protein motif:HMM:NF025032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOD87709.1"
                     /translation="MKTRLFLLLGLMFIFSINSYAQNLGANNSKMIIGKWKVDSLDVG
                     DSPLAIQYKPIIEEKMAQIIETTEVLFYPNKKYLKKGFDGTTEGTWRISKDGQYVSVK
                     IKGRDKEDRTKIITLTEDKLIIAPEDPNAVNAKVYMYKVK"
     gene            complement(23887..24999)
                     /gene="recF"
                     /locus_tag="PKN53_01755"
     CDS             complement(23887..24999)
                     /gene="recF"
                     /locus_tag="PKN53_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005939955.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOD87710.1"
                     /translation="MNMHLKNLSLINFKNFESFELEPCAKINCLIGDNGIGKTNLLDS
                     IHYLSFCKSFLNPSDRQNIKTGEDFFVIKGKFELDDNVENIYCGVQKDKRKSFSRNGI
                     EYQKLSEHIGLLPVVCSSPYDSDLIHQGSDIRRKFADSIISQFDKSYLNNLINYNKVL
                     EQRNILLKNSFSTKRIEPDEIEIWDFKLSENAEKIYEVRKQFAEELIPVFQKYYTLIS
                     DNNEKVELVYSSQLHKNNLYDSLRKNLERDLALGYTSYGIHRDDFDFIIENQPIKRYG
                     SQGQQKTYVISLKFAWFEYIRNKLDMAPILLLDDIFDKLDKKRVKVITELVADNEFGQ
                     IFITDTSYSRMPDILKNLDISYKVLNLSTNEIITNK"
     gene            complement(25167..25523)
                     /locus_tag="PKN53_01760"
     CDS             complement(25167..25523)
                     /locus_tag="PKN53_01760"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB domain-containing protein"
                     /protein_id="HOD87711.1"
                     /translation="MFAKYRANLEENYANVVVIVNNPCLEFWFLLHFKKTSKYYDTCA
                     EAKTELKKYLKNYEKTRKFFTKQGDDIYLKLKPRLKIAVENSIALGNYDKKNPKKAMC
                     EMELFFQSSELKKHFE"
     gene            complement(25593..25808)
                     /locus_tag="PKN53_01765"
     CDS             complement(25593..25808)
                     /locus_tag="PKN53_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87712.1"
                     /translation="MRKPKRYAPKPPTSIAVVVDGDDEVWYLQMLKRNEPKIRISIKP
                     EIPNKKSVEEQYNLVRDLSEKEFTKVF"
     gene            complement(25805..27109)
                     /locus_tag="PKN53_01770"
     CDS             complement(25805..27109)
                     /locus_tag="PKN53_01770"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD87713.1"
                     /translation="MILNFNVQNFGPIKDEQTLSFEADNSTHLEDAYVANIGGQRILK
                     LALIYGANPSGKTTILKALDFLRQIVLEPEDKKTTELDFSPFLFDSITPKQNSIISLE
                     FFQNEIKYFYEVEFCKKAIVSESLYFHNPNKANIFKRKTDLKKQFTEITFGSKISADK
                     VFKKTLEANTLWNNTVLGGYLKTNIDFKELQEVIDWFVKYLNPLIYARTQLEGFVTSQ
                     IDNKKISKSDVVSILKKADLNISDIVIQKEEKDFPGAFIEFLKNQVNVPSDKARELEE
                     KGKLTAINVEFEHEVNNVKYTLPLDEESEGTRRYYGFAGLLALLIQNSTAFLIDELEA
                     SLHPDLYLHFLISFLLNSENSQIIATTHNREILDNRDVFRNDVIWFTDKQENCSTELY
                     SLADFDSSVVRNTTNVLNAYKSGKLSATPNLGDTFIDFNVKL"
     gene            27333..28568
                     /locus_tag="PKN53_01775"
     CDS             27333..28568
                     /locus_tag="PKN53_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOD87714.1"
                     /translation="MTSEILFIIIITIVVLDFFVEQILEYLNASWFNKPIPEILKDIY
                     DTEKYKKQQSYKSENYRFGIISSSFSFILIVLMLVFNGFAYVDTLAGQISENFILKPL
                     VFFGIILLAMDILSTPFSVYDTFVIENKYGFNKTTVKTYILDKIKTYLLGGTIGAGLF
                     ALILWIYHLDPEYFWLYACLVIIAFSLFMNMFYSTLIVPLFNKQTPLEDGELKTKIME
                     FSKKAGFNISKIFVIDGSKRSTKANAYFSGIGPKKRIVLYDTLINEMTNDEILAVLAH
                     EIGHYKHKHIYWSMIAGIIEIGIMFYIFGLFASSDMLSQALGLEKASFHIALIAFSII
                     YSPISSAFAVVSSIFSRKAEYQADNFASKHGLSNELISALKKLSSHNMSNLTPHPWVV
                     FMTYSHPPVFNRIKALMKH"
     assembly_gap    28751..28850
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(28969..30963)
                     /locus_tag="PKN53_01780"
     CDS             complement(28969..30963)
                     /locus_tag="PKN53_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD87715.1"
                     /translation="MKKFTILMAFVALASFAMAQAIQAPSNIACKNIEKDTRTMVEKY
                     MERQALKANVAWTQDFDGAEAWTTYANPVVGQYKWQTVNVEQFMAAGWGDFENGWYLF
                     PMADSATDANKWAFIDIYGPILRAATADRPDSVANMPVVFEAYIQFDDIDLSTVNNPM
                     LLWNQYYRTFNASMQKLYVDVSVDGATWTSYQINKSADIAHGEYAEYEQALLLGIAAN
                     ESNVSIRFRWTHDRTGYEIYATGYAWGIDDVMVIEAPQYDLKHLETAMNFFVYIDYHE
                     AGNANGYHYSGHFGQIPKEQMDTEASYMFFNTIVENNGLQVATPIVKVEVTNPEGTVV
                     YNNTITGPEIASGAVDTIDIMAPEFGLEAPIVTGEYVASIEITIQGQEDQNPNNNLYE
                     TRFFVTGDGRYARDLDNITQSYGLAAWNIGGNNEEMLATKYFINETTTIESVDVYIEG
                     DPDAENWGTIGNTVQLVLLSWGGEEYVAFAESEPLILAAEHLNTWVNFTFADNTEIIV
                     DLVNEGVGEVFVAVKMFYTGSYSKNTIDIGMDKSTNFGAWSGWYRILYGGEWGWYYGA
                     SDGKAFAIRINLPHDNYVKEDVVANFKVYPNPTQDILKIETQSQKSILNIYSVDGKLV
                     RNVEVSNNAEINVSEFATGVYTVVLTDENGNVGTQRFIKN"
     gene            complement(31051..31812)
                     /locus_tag="PKN53_01785"
     CDS             complement(31051..31812)
                     /locus_tag="PKN53_01785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD87716.1"
                     /translation="MLKLSAVIITLNEEKNIGKCLHSLQTLTDDIVVVDSGSTDKTEE
                     ICKQYNVKFFVRDFDDYSSQKNYGNSFAKYDYILSIDADEVISEKLRSSIEKIEFGEN
                     EFYGFNLLNHYCGKPIRFGGWYPDKKYRIWNKNYGKWEGKIHEKIVFSQPPKKCFLKG
                     NLLHFTYESKEIHKQKTIKYAKLMAQKDFENGKNYGYLMLYLKPIYKFISMYIIKLGF
                     LDGKRGFLLAKTSAYASFVKVFEFFLKKKHKNFCI"
     assembly_gap    32195..32294
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <32295..32506
                     /locus_tag="PKN53_01790"
     CDS             <32295..32506
                     /locus_tag="PKN53_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87717.1"
                     /translation="NPSERGGLQIRASGEDYKSERAGRIANPSERGGLQIRASGENKK
                     FYIYIIKAKHIFFMLFAQKIRLYEL"
     gene            32627..33274
                     /locus_tag="PKN53_01795"
     CDS             32627..33274
                     /locus_tag="PKN53_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HOD87718.1"
                     /translation="MNKIITVEEAVKKINDGMTIMIGGFLAAGSPHTIIKGLVEKNVK
                     NLTLIVNDTGFADKGIGLLISNRQVKKVIASHIGTNTATLEQFNNKELEIEFVPQGTL
                     AERVRCGGAGLGGVLTPTGLGTVVEEGKQVINVNGKDYLLETALRADVALIGASIADE
                     SGNLYYKGTTQNFNPLMATAADLVIAETKEMVKIGEIAMENVHTPALFVDYIVKS"
     gene            33287..33679
                     /locus_tag="PKN53_01800"
     CDS             33287..33679
                     /locus_tag="PKN53_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HOD87719.1"
                     /translation="MKTTSMIRLRMGTNDAHYGGNLVDGAKMLQLFGDVATELLIIND
                     GDEGLFVAYDNIEFLAPVYAGDFIEATGEIVSQGNSSRKMVFEAKKVIKPRTDISASA
                     ADFLEEPIVVCRASGTCVVPKNCQRKNK"
     gene            33686..34510
                     /locus_tag="PKN53_01805"
     CDS             33686..34510
                     /locus_tag="PKN53_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOD87720.1"
                     /translation="MDKLIITAAICGAEVTKEHNPAVPYTVEEIVREAKSAVDAGAAI
                     VHLHVREDDGTPTQSKARFKENIDAILKACPEVILIPSTGGAVGMSPEERLQPTELPI
                     EMATLDCGTLNFGDEVFENTLPMMRAFGKRMIERNIKPEYECFEMGHVEAVLDMVRKQ
                     QSPGAPLHFNFVLGVPGAAKATINNLVWMVNAIPQGSTWCATGVGRHEFPLAAHAIAM
                     GGHVRVGFEDNLYLERGVLAKSNGELVAKVVRMAKELGREVANFAEAREILGLKQK"
     gene            34533..35576
                     /locus_tag="PKN53_01810"
     CDS             34533..35576
                     /locus_tag="PKN53_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOD87721.1"
                     /translation="MQKKGNKYGLHRVIEPIGVLPQPAKKVNNNMDEIWDNEILIDVQ
                     TLNVDSASFTQIKEQAGGDKAKIAEIMLDIVAKQGKHRNPVTGSGGMLLGTVEKIGDA
                     LKDKIDLKVGDKIATLVSLSLTPLRIDKIKEIRPDIDQVDIDGKAILFESGIYAKIPN
                     DMPEGLALSALDVAGAPAQVAKLVKPGDTVLIIGAGGKSGMLCCYEAKKRAGVTGKVI
                     GIGHSERSTKRIMDLGLCDHVFSADATQPVPILEKIEELTKGEMCDVTINNVNIEDTE
                     MTSILCTKNSGTVYFFSMATSFTKAALGAEGVGSDVTMIVGNGYTKGHAEITLQILRE
                     SEKLRKVFNELYA"
     gene            35653..36888
                     /gene="ablA"
                     /locus_tag="PKN53_01815"
     CDS             35653..36888
                     /gene="ablA"
                     /locus_tag="PKN53_01815"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HOD87722.1"
                     /translation="MNEGRKKMFPNATDEQWNDWKWQVKNRIETLDELKKHIKLTKEE
                     EEGVAKSLKTLRMAITPYYLSLIDPKDKNCPVRRQAIPTGAETHQSAADLLDPLSEDE
                     DSPVPGLTHRYPDRVLFLITDMCSMYCRHCTRRRFAGQTDKASPNDRIQQGIDYIART
                     PQVRDVLLSGGDALMVSDKMLESIIQRLREIPHVEIIRIGTRTPVVCPQRITDDLVNM
                     LKKYHPIWLNTHFNHSKEITAESAEACAKLADAGIPLGNQSVLLRGVNDCVYTMKELV
                     QKLVKIRVRPYYIYQCDLSMGLEHFRTPVSKGIEIIEHLRGHTSGYAVPTFVVDAPGG
                     GGKTPVMPTYIISQSPGKVVLRNFEGVITTYTEPTDYKEECCCKDCKEKTKNEGVASL
                     LRGEQLALEPKELARKKRF"
     gene            37222..38232
                     /locus_tag="PKN53_01820"
     CDS             37222..38232
                     /locus_tag="PKN53_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87723.1"
                     /translation="MPFLKEILKYKSLSIIGTEKNTGKTECLNYIIQSLKNSNKTIAI
                     TSIGIDGESVDAVTETPKPEIEIYKNNIFLSSEKHFLQKQITAEILDVSEKQTSLGRL
                     ITARAMDTGKLIFSGPPDTKWLKEEIQNSQKYGVDLFLVDGALSRKSTASPSVTESMI
                     LTTGAALSANIPTLVKKTKFICDLIELPVFETNLTNELLQIKNGLWAIDSDNIIHDLD
                     IKSTLLLDKAKDKLYSKGCVIFASGIITNNILEFMRIQKNIKETILIVKDFTKIFVSQ
                     EKYNSFTKAGGKIMVLLKPNLISICVNPWSPRGIVLDSQKLREELFKAINLPIYDIKA
                     LD"
     gene            38232..39614
                     /locus_tag="PKN53_01825"
     CDS             38232..39614
                     /locus_tag="PKN53_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOD87724.1"
                     /translation="MKSFRKYISEIAGLRFVYENLELNSSAGRKKLLKSQLFTKEFEL
                     TVNIQALSEIYDFIQDKKNKKIVSDICTTLNQVNDISASINNIRFNRVSDDIELFEIK
                     KFCINSQKILKQISPFDFSLILLHDLSEVITILDPQNTGIPAFYIYSDYSPELAQLRK
                     EKDKAKTPEEAENLRLKCVEIEDKIRENLSKQLANYVNKLEYNLNRLADFDILIAKAK
                     FAVKFRLTEPKITKEKTIYKGLFNPQIKTILESQNKNFQAIDIEIFPFPTLITGANMS
                     GKSVLLKTVALSQFMFQFGFFVPTQNAEIILVEEILLNIGDNQDELNGLSSFAVEILN
                     INKIISHAKSGKKILALIDEPARTTNPDEGKAIANAIVEILTELKVNSLICTHYSGIK
                     ANKKLRVKGLQNISDNENINIENINDFMDYSLIQVDEEKAPAEAIRIAEILGADKDFI
                     EKAKKEVNNI"
     gene            39749..41518
                     /gene="sppA"
                     /locus_tag="PKN53_01830"
     CDS             39749..41518
                     /gene="sppA"
                     /locus_tag="PKN53_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOD87725.1"
                     /translation="MKQFFKYTLASLLGSILTFAILIGILIAIISAFVAGFSEQEAII
                     DDNSVLKLRLATDIPDKASDNPFSNIDFISMTSTKTMGLNNILKNIEKAKKDDKIKGI
                     FLDVGSIPSGLGTVEEIRNKLIDFKESGKFIIAYSSGYTQKSYYLASVADKIYLNPEG
                     SFDWKGLYSQVIFFKGALDKLGIEAQIFRHGQFKSAVEPFFTDKMSEASKLQTITLIQ
                     SIWDDMCAKISKSRNIEIEQLNLIADSLWAYDAKTAKEYKFVDGLVYYDEVLAELKTK
                     SASEEAPEDFIVSLGDYASVKIKTKANNSKDKIAVIFAEGEIIDGGKSEGSLAGDWMS
                     EIIREVREDEDIKAVVLRVNSPGGSGLASDIMWREITLTKEKKPVVVSMGNLAASGGY
                     YIACPANYIFALPSTLTGSIGVFGMLPNVEKLMTEKLGITVDGVGTNAQSDFGQITRK
                     TTTSEANLIQNQVEEFYDTFIQKVADGRGLTKAEVDSIGQGRVWSGLNAIEIGLVDEI
                     GGLNDAVAKAASLANLSEYRLIEYPQKEDAFETFIKDYNKNVTEKVIKETLNKNYKFW
                     QIIKNAQNMSGIQARMEYELEIN"
     gene            complement(41913..42173)
                     /locus_tag="PKN53_01835"
     CDS             complement(41913..42173)
                     /locus_tag="PKN53_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87726.1"
                     /translation="MGNKYLLLILGLLTTVLGLLTIFFSIYGAYLKINHLENGNLFLI
                     ISVCIAPLFVLSLAFFINKILKKKQKKSFNLMTMLSIFEKYA"
     gene            complement(42223..43014)
                     /locus_tag="PKN53_01840"
     CDS             complement(42223..43014)
                     /locus_tag="PKN53_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HOD87727.1"
                     /translation="MAKKRRRSRKKKNKNRRNIFLVIALAIIAFLTYWYYEEIQSFFF
                     PNQHVKGNFSKLKLEGYSVYGIDVSQYQGKIDWKALKTEEKPDFVFIRACAGKNHKDT
                     KFDYNWKQAEKQNLLRGAYHYYRPNENSTEQANFFIKTVKLEKGDLPPILDIEKYSRI
                     QNLPKLKDGLLNWLQIVEEHYGVTPILYTYHNFYLNTFVHDKRFDKYPLWIAWYNTKK
                     NPNKINKDWVFWQFTDRARVKGIKGDVDMNVFNGNLQDLDGLRLR"
     gene            complement(43014..44006)
                     /locus_tag="PKN53_01845"
     CDS             complement(43014..44006)
                     /locus_tag="PKN53_01845"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOD87728.1"
                     /translation="MKLAVVGVTGMVGASLLKVLEEYKFEFDEFIPVASEKSVGKSVY
                     LLGKEYKVVSLEQALEQKPDIAIFSAGKSVSLDWAPKFASNNCFVVDNSSAWRMDKDI
                     PLIIPEVNISILENYNKIIANPNCSTIQLAVVLWALHLKYKLKRVVVSTYQSVTGTGH
                     KGVNQLMVERAGKKPENMAYPHQIDLNCIPQGGEFLENDYTEEEMKLVHETRKILNLP
                     ELKLTSTVVRVPVLGGHSESVNVEFENEFDISELRDILEETDGVSLLDNPQKSIYPMP
                     LTAENSNLIFVGRVRRDESCENAVNMWIVADNLRKGAASNAVQIAQYICDNFIE"
     gene            44206..45396
                     /gene="kbl"
                     /locus_tag="PKN53_01850"
     CDS             44206..45396
                     /gene="kbl"
                     /locus_tag="PKN53_01850"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOD87729.1"
                     /translation="MYSNYQKFLQEEISEIKKAGLYKSERVIVSPQGAEIEVSSGQKV
                     LNFCANNYLGLANNPKLIEAAKNALDTHGYGMSSVRFICGTGDLHKELERKIANFFGT
                     EDTILYAACFDANGGVFEPLFTQEDGIISDTLNHASIIDGVRLSKAQRFRFKNADMED
                     LEKQLIAAKDCRFKIIVTDGVFSMDGNVAPLDKIYELANKYDALIMVDESHSAGVVGK
                     TGRGITEHFNLMGKVEIITGTLGKAFGGAIGGFTTGKKEIIEMLRQRSRPYLFSNSIP
                     PLVAAAGIKMIEMMSETNELQDKLHDNTEYFMKKMSKLGFDIKPTESAICAIMLYDAK
                     LAQDFAAKLLDEGIYVIGFSFPVVPRNEARIRVQLSAAHEKEHLDKAIAAFEKIGKEM
                     GVLK"
BASE COUNT        15847 a         7298 c         6566 g        15756 t
ORIGIN      
        1 ttcaactttt aacacttcaa ctacgttcag tgcatcgctt ttaactttta actgactgtt
       61 ctatctgcgg agtaagcgtt tattaacaaa atccaagaaa acatcacgat aagatcttcc
      121 tattggaatt tcgttttcac ggatattaat aacattaccg tctatatcgt caatgtggtt
      181 tacattgata ataaaagatt tgctcactct aaagaacata tcttctggca atttgttatg
      241 aatagttttt acattcatag cagtcatata tttgccatga gttgcatgca ctataacata
      301 atctttcaaa ccttcgacaa ataataattc atcaaatctg attttaacat attttctttc
      361 agatttaata aatataaaat catcttcaac agcttcgaac acagcagtat tttcagctaa
      421 agataaaaaa gtttcagctt tagcaactgc tctaataaat ctgtcaagtt taataggttt
      481 tacaagataa tcaaccacat caagttcgta tgcttctaca gcatattgag gataagcggt
      541 tgttaaaata accattggtg catcgtcaag acttctcaaa aaatccaaac ccgattgtcc
      601 tggcatttct atatccaaaa acataatgtc gacttggttg ttttttagaa attcgtttgc
      661 agaagctgca tcttcaaatt caccaactaa agttaagttt gatagttcgc caatattcat
      721 tttcattcct tcgcgtgcaa taggctcatc atcaacgata atacatttca tcattttata
      781 taaagtttaa gttcaacaat aaagtaatta tcaaaatttt tcatttttaa ttcataattg
      841 tgtttataaa aaaactctaa gcgatttttt atttttataa aattttcatc attttctaaa
      901 tcaaaatttg aacttatcgc attttccgaa tttttgacgg taaagataat atttttttct
      961 acaactgcaa tataaatatc aataaatttt tcattattac tcacatttcc cactttttta
     1021 aaagccagct cgataaatgg caaaaacata aaaggatata ccattaatcc actaaaatta
     1081 cctttttgtt caaattttat gtcaatgttg gcaattctca tcttttgaag ttctatgtaa
     1141 ttttgcaaaa attccacttc tgatttaaca aacacttttc cttcaacaca atcataaagt
     1201 tggtatctta aaatatccga gaatttcaaa agcatttctc ttgctttttg agggtccact
     1261 tgtatttgta tataaatatt attaaaaaca ttaaacaaaa aatgtggatt tatttgattt
     1321 ttcaattcat ctagttctgt agaaatttta gcattttgtg catgggttat tgtttctaac
     1381 ttatctctat aagtaagcaa agttttcata ataaatgcaa aacttataaa aacaaaagat
     1441 aagataaaac tgtatgattg tttaattaaa catgtgctaa aacttgattg aaaatctcca
     1501 aaaataaagt tttgcaaaag aaaattaatt ccaaaattaa caaaaagtac tgccaaataa
     1561 gcatgaaata ttttataata tgttttttct aaaagtttga ccttatcaac ataatagtaa
     1621 actaagaaag taattgaaag taaatctatt aaaacaattg acaggaatcc ctcaaatggt
     1681 acagatatta aaccgctaaa atatagactt aaagcactta aaaaatagaa aagccaaaaa
     1741 actacattaa caaatgctat caaaaaatta taatttgatt ttttttgcat atctcttcag
     1801 tttttacaaa atccccaaat ataagcattt caaacataca aaatgcaaat taaatctaat
     1861 tttttgccaa ctaataatta ttttgttagc aaaactttaa ttattgctat gataaagttt
     1921 agttaaagga ttgtatttat aaaaatcaaa tcatatttct atacccgaaa tggatttttg
     1981 atgcattaaa cataaaacta tttgagttta attttcctac agcccagact aatgaaaaga
     2041 ttcctgctcc tgtatttagt tctattcctg ccccaaagct gactgcataa ttatattctt
     2101 tatttattgt atttcttttt tcgaaataag caaaatcgca caaggcaaaa acagcagaat
     2161 tttcttcaat tctataaagt atttctgtgc tcgcaataga atatgagctt gctggtaatg
     2221 acaagtcgtc gaaacctcta atagttttca aacctcctat cctatccaat tcattttcaa
     2281 acaatttttt actaaaaata ctcgaagtgt tagatttgaa tttaaaaatt aaatttctat
     2341 aaattggtaa atatgctgtt agctcaagtt tgttttttgt ttggaaaata ttttttttat
     2401 cttcttcaat aaaatcttcg tttttaagtc caaaaccact attcaaaaac aaaacaattc
     2461 ctttattcgg cgttaaaatg ttatctatat taattaatct aaagccaaaa ccaaaaagtt
     2521 tagatttaac actacttaat ccgtcagtgg cagaacttaa aacaattgag tttttatttt
     2581 caagaaataa gttaaatcct gttgcatttg aatttccata aattatttgt gcatcaaaca
     2641 atgtgtttaa ccaagtgcta tctttttttt ctaaattaaa cccaagtcct attccaaaat
     2701 cagatttaaa aatataggga tacaaaaaat cagtttctaa attttgagaa aaagcatcat
     2761 attttttcca atttaccgac aaaacttcgc cacgagcaaa agaattaact aagtttaact
     2821 gtaaatcgcc tgttagcaaa agttttcctg tagttatatt atttggtaaa attcctaaaa
     2881 ctccgctaaa actacctgct tttttatttt ttaaatataa taaaatatct gacttattct
     2941 caccaaaagc aagttgaaaa gctcgtgttt gctgcaaaaa tggaatgcca gaaattgatt
     3001 tatttatgtt tttaattttt gaataactaa aagcatcgtt ttttttaata tttaatacct
     3061 tattaatata atatggttta attttagctt cggacttgat aattatacta tcaaaaacaa
     3121 aatattctcc tgaatcaagt tttattatag cagatattga atcgttttca atttcaatat
     3181 tttcaatttc aaattttgca aaaggataac ccttatctgc taaaactttt aaatattttt
     3241 catgattaaa acttgggtca aaaaatgctt ctttatcaaa atccttaaaa ttcaaggttt
     3301 tagcaaggct atcaatctca aaatcaagtt ctttaagaat atatttttga cctgaattat
     3361 aataagcatt tactaacttt tcggcataaa caatgctatc atagcctgct gaataaaatc
     3421 cttttgaaaa ttctgataat aaaaactgat ttaatttatt ttcagcatca acactatcgg
     3481 taaaattctg agtttttaaa gaaatttgtt cagaattttc agaattaatt gtaaggatat
     3541 ttaactgttg agcaaaattt attttttgta taaaaacaca aaaaactata aaaagaagag
     3601 aatatctaaa tattaatttc attcatttcc tatactttgt atagaaccat aattttcgtt
     3661 aaatattatc tttagctttt tatttttaaa catttttatt ggcaaatatt ctatttgtcc
     3721 gaactgaggc actacaacat tagctttata ataatcaaca ttttcgactt ttacttttat
     3781 tgttgcattc cctggtattc tgtaataaag tccttgaggt ttatttgcct tagttgaatt
     3841 agctttgttt ttataaaaat tagagatttt agaatgtttt tgattttttg taagctcaaa
     3901 ataaacttgt acaggttctt cggcatgtaa atttaaaagt tttacctttt gggtttcaac
     3961 atttccatcg ggaataattt caaaatcata ttcttgatat ttatattcaa cttttccaat
     4021 aaaaagcgac aaatattctt tttctaattc ttctaattta gcaaccataa attccaattc
     4081 tgttggtgga ttttcccatt caaacattcc tgctaataaa aagaactttt cttcgcgtat
     4141 tttaagaata aaattagcag cttcttcggc tttttgttca aaagttttac ttgttatact
     4201 tgtattataa acaggaattt tttgatatat agagtctatc tgaacgacct tataagttgt
     4261 atcagtaatg tttgaaaagt ttttttcaac cgtcaaatct gtaaacaaag gaaaatgaaa
     4321 attagaaaac tcaaaacttt cacttaacaa atcactgtct aacaacaggt tatcatcgct
     4381 atctatttgc actccatacg aagatattac accattttca tttagataaa ttttaggttc
     4441 tttttttgat tgtactacaa aacttgcatc aaaatctgta atatatttag gaattatttc
     4501 aatatttgaa atatcagaaa actcagaata ttctgtttta acatcgctaa ttatcaaata
     4561 tttttcggca tattgggcaa atgggccagg ataaaaacat tctgttgcaa catttacttt
     4621 aacttgaaaa acaactttag gtaaagaata cacatacata ttttctttaa ccttcaattc
     4681 ctcattagaa atgctataaa ctttaatttg agcaaacgaa gcatttacaa aaccaaaaaa
     4741 aactattaaa aacactaact ttttcatatc gtacactctt tttttgcaaa aataataaat
     4801 cctctgaaat aataagcatt ataaaaatta gtttaagtaa acctcatcat cttcgtcatc
     4861 agtccaataa tcgtcatctt cgtcatcagt ccaataatca tcttcatcgt cgtcagccca
     4921 ataatcatca aaaaactcgt catgttggtc tggcatatta tgtgcttcat caaacaattc
     4981 tgatagtggc gaatttacat ttctaagata attaaaacta tttcctaaat gatcaataat
     5041 atcagaagct atcatagttt cttgactttt tacaaaactg gcaaaatctc ctgcatttgc
     5101 atgtatatac acagccaaca aagctgcatt ttgagcagaa taattttgag ctaacaaaga
     5161 agttatcatt cctgtaagca catcgccaga accagctgtt gccattcctg gattacctat
     5221 agtattaaaa tacactttag cttctggtgt cgaaattgct gttactccag attttctaac
     5281 aattataatt cctgtttttt tagaaaaatc tcgcataaat tcaattgctt taaattcaga
     5341 gtcaaatttt ccaaataatc tttcaaattc tttaggatgt ggggttaaga ttgttcctgg
     5401 ttttaaatat ttaagcaatt ccttattttc agaaattatg ttcaaaccat ctgcatcaat
     5461 aactagaggt tttgtattag tttttagaaa atttgcaaaa gcattttttg taatatcatt
     5521 caaacctaag ccaggtccaa ttccaattac gttatacttt tcaaaatctt caatctgaga
     5581 aaaatatttt tcattttcat caattgaaag cattgcttct ggaatagaaa tttgcaaaac
     5641 atcaacaagt ttttcgggta catgtacagt tagcaaacct acacccattc gcatacaagc
     5701 ttttgctgcc aatattgctg ctcctccttt tccatacgaa cccgctatca acaaggcatg
     5761 tccaaaatct cctttatgag caaaacgttt tctatccaca agcatatctt gtggcaaatt
     5821 cttactaata acataaaatg gagtttctaa tttttgctct atttcttcta aaaagcctat
     5881 ttttacacaa attgtttcac caaaatattt ttcattttca gcaaataatg atgacaaagg
     5941 caaataacca atatttaaag taaaatctgc tcttacttta gctccattat tttcagaatt
     6001 atcttcgcca aacaaaccag aagggatatc cacagaaact acagtatttt gagactcatt
     6061 gattttttca ataacttcag catatttgcc aagcacttct ctattcaaac catttccgaa
     6121 aatacaatct ataatcactc tgtcttcaaa atctggtaat tgctcaacat cagttatgat
     6181 atgtaagttg tgatttttgt ttctctttaa tttattcaaa tttgttttac attcaggact
     6241 aatttttgaa gagaactgac aaaaaacagt aagcacatca aaatctaagg ctttatacat
     6301 acgacttaga gctaaaccat ctccaccatt attaccagga ccgcacacaa aataaaatct
     6361 tgtttctcta atatcaatag tgtcatcttt tttgcgtaag cactccacaa ttgggtctaa
     6421 caattctgca gctgcttctt ccattaaatc tattgaagca attggtctat ttttgatagt
     6481 gtattcttct atttttttta tttgttcaga tgtaaatact ttcatgattt ttttcgttaa
     6541 aaataaaaaa tattccaata catataaaat gttattaaaa ttaatttaaa acaaaatgaa
     6601 atatcaaaaa cattcctata tttgcaaagt taaataaaag aattattaat taataaattt
     6661 tagattatgt ttagaaatca tcctaaggga cttttaggag catctttagc gaacatgggt
     6721 gaacgctttg gtttttacac catgatggct attttagttt tgttcttaca agccaaattt
     6781 ggtttaaatg gaaaagatgc gggtcttatt tattcaattt tttatttttc catctatatt
     6841 ttagcttttg taggtggttt aatagctgac agattgagaa attacaaagg agtaatattt
     6901 gttggtttga ttgttatggc gactgggtat ttaatgattg gaattccaac agaaactcct
     6961 gtagcaaatc aactaactat gctagttatc acttgtgtag gcttgtttgt aattgcattt
     7021 ggtaacggtc ttttcaaagg aaatcttcaa gcccttgtcg gacaaatgta cgacaacgaa
     7081 aagtacggaa aaatgagaga ttcaggattt tctttatttt atatgtttat taatgttgga
     7141 ggcttttttg ctcctttcac tgcaattgga atcagaaatt ggtggttaga aaggcacggt
     7201 tatgcatacg atgcaaatct tccagcactg tgtcatcaac acatagacaa cacattagaa
     7261 gcaacaaaag ttgttggcac aggcgcttta gacaaagctc aaggagttgc tgaaaaagta
     7321 tttagtttag accctattag taagtttcaa aatttagctt cagaagttgg aggacaaact
     7381 gaaaacttaa caaacttcgc aaacgactac ttaaatatat ttacttctgg atttcattat
     7441 gcttttattg cagctattgg tgccatgatt atctcgttaa ccatttattt attcaacaaa
     7501 aaacacttcc ctaatgttga taaaaaagct aaagctacag aaagccaaga ttctgaaatc
     7561 aaaatggatg ctaaagaagt taaacaacgc ttatatgcat tgttagcagt gtttggcgtt
     7621 gtaattttct tctggttctc tttccaccaa aacggcttaa cactaactat gtttgctcgt
     7681 gattatacag atttatcggg aattaaaatt aatttaggct ttgtaacaat acaaggagct
     7741 gaaattttcc aatccattaa tccatttttt attgtattcc ttactcctat tataatggct
     7801 atatttggat ggctaagagc aagaggtaaa gagccttcaa cacctaagaa aattgcaatt
     7861 ggaatgggaa tagctgcaat tgcttacgtt gttatggcta taggctcata cgacttgcca
     7921 ttatttaaag acatacaaag tagtcctcta gataatgcac ttaaagttac tcctttctta
     7981 ctaattggaa catattttat tttaacagtt gctgaacttt ttattagtcc actaggaatt
     8041 tcttttgttt caaaagtggc tccacctcat ttgcaagggc taatgcaagg tggttggcta
     8101 ggtgcaacag caatagggaa tcaactacta tttataggtg ctattttata tgaaagcaca
     8161 ccactatggt taacatggac agttttcgtt gttgcttgct tattgtcaat gattacaatg
     8221 ttagcaatgt taaaatggtt agaaagaatt actaaataat taatttaaat tttataaaaa
     8281 tgaaaaagat aatatatttt tttgttattg ttgctttatc acttacgaca atagcctgtt
     8341 caaaacaaaa tgctgtagaa gatgttgaac gaagtattgc caaacttaat caatttaatt
     8401 ctacatttga aaaattttat aatgacggtg ttatagacgg aattaacaca aagggagata
     8461 aaaataaaga tgaagattca gagtataacc agcttaaaaa attagctgaa gaatattatg
     8521 aattgattaa tcgtatcaat tctaacatca atgaagataa agaaaatgtt aaaaaaggta
     8581 agaaatcaaa tggctacgaa gatgaatata aaaaagctgt tgaaagtaaa aaagatgaaa
     8641 tagaaaaagc tactgattta tttattaaaa accttagtat cttaaacaat tgttccgaag
     8701 actataaaat aattgaagaa gaaacacaag atgttattac tgaagaaacc gttgaagaaa
     8761 ctgttgaata gatagaaata aataattata aaataaaaaa agttgcaaaa tatttgcaac
     8821 tttcttttat taatagtaat tttacaatca ttaattttaa tacattatat ttatgaaaca
     8881 aatttttaaa tttattggtt taagtttagc tttaatgcta ttttctattg tgatttttgc
     8941 acaacaagat tgttcaaatc cacacatgat cactacaatc cctttttcag caactggact
     9001 aagtactgaa ggaatgggaa acttatttga tgctgacgat ttatgcacat ctaacgctat
     9061 ggtaaacgaa gaatatattt ttcagttcac tcctgcagaa gatatgcaaa ttaatattgc
     9121 tttagaaaat acagaagtaa cttattcagc tttccctatg gctactgatg atgcaaacat
     9181 aggactgttt ttaactaaca aatgtcctga tgaacctgca ggaacttgca tagcatctgt
     9241 tgacaaccaa accagcaatc cttccttaaa caacattaac gtatctgcag gtcaaaatta
     9301 ttttataatc gttagctctg ctaatgtccc atttttaggc ggcactaatg taagttttga
     9361 cataaaaata acaagaattg tagattatga tttagaagta acatctattt ctctacctaa
     9421 tagttcttgc agcttaggtg atgaacaaat ttcatgtgta ataaaaaata atggaacatc
     9481 taatgttact agtccattta atatttcata ttcggtaaat ggtggaacat ctgttgtaga
     9541 aagttgctca gatcctatta attcaaattc agaaataact tacacattta caaatcttgt
     9601 ttcgtttact aatactggcg aatacaatat tgaagtaaca atagacttcc cacttgacga
     9661 aaatgtaaac aacaactctt taagtgcaac aacatattat ttgccaactt atacaagttt
     9721 cccatttact gaagattttg aagccggtaa gggcttttgg tcaactggtg gaacatcatc
     9781 aagttgggct tatggcaatc catcggcatc accagaagat cttgtgataa actcagcagc
     9841 atcaggacaa ttttgttgga taacaaattt aaacggcaat gcaaatacta acgaaaattc
     9901 atatatcgaa agtccttgct acgatttatc tgcactgctt ctaccttgtt tagaagtaaa
     9961 ctattgggga gattttgcta tatatggaaa ctctgcaagc ataatagcct ctgccgataa
    10021 tggcgcaaca tgggacacaa ttgctaatct gaacgcttct gaaaactggg aaactcttat
    10081 aattgcactt cctaatttag ttaacgaaac aaatgttaaa tttagagtta ctttcaaaag
    10141 tagtttcctt gttgctaacg gtttagctat cgatgatttc acaattaaag aatctgtttt
    10201 aaaagatgtt ggagttagca atattattag tccaaatact ggctgtggac tatccgatat
    10261 agaaacagta ataattgaag ttactaatca cggtatccaa gctcaagaca atattcctgt
    10321 aaattattct atagatggtg gggtaacttg gttagcaagt gacgaaatta ttccttctat
    10381 cgtttcagaa ggtacagtac aacacacttt tactattact ggagatttta gcgaaattgg
    10441 agaatatgaa attattgcca aaaccaatct tacaggagat gaagttgctg acaatgatag
    10501 ctttacaaaa actgtttata aacaaaacac aatagttgtt accgatactt attcagaatc
    10561 atttgaaact ggaaacgctg gctggctggc ttatggaaca aattctagtt tagagttagc
    10621 tcaacccaat ggcactataa taaaccaagc tggagatggc gaatacgctt gggtaacaaa
    10681 tcctaacggt ccgcataata ctaacgaaac ttcttactta gaatctcctt gttttgattt
    10741 cagtacccta acaaacccaa tatttaaaac aatgatacag tatgaaacac agatgataat
    10801 gtcaaatttt tatgttgaat attcaacaga cggaatagct tgggatactg taagggcagg
    10861 aactgtttca aataactggt atggtagtgg ttttatgagt tttggaactt ggggtggcgc
    10921 atcagaaggc tggctagatg cttcgactga aattggtttc ttagctggac aatcaagtgt
    10981 aaaatttcgc tttgtattta attcatcatc attcccatgg ggtggagaaa ataccgaagg
    11041 tgtggctata gattttatta gaattgaaga atgtgcagat attccagaag ccatgttttc
    11101 attcgaaaca aaccaactac aagttcaatt taccgatgaa tcaacaaatg ccaacacttg
    11161 ggaatggagt tttggagaca acgctttcat accttcaact tcaaccgaac aaaatccaag
    11221 ttttacttac tcacaagacg gacaatatac tgtaagctta acagtaacaa acgcttgtgg
    11281 tgtatcaact tatgaaacta ctgtaaatgt tactactact aatctaaatg aaaattacaa
    11341 ggatttaact aatatttatc caaatcctac aagtaatttc ataaatatcg aaaatattca
    11401 aaactcagaa ataaatattt atgatgtaaa tggaaaatta gtctattcta aatttataaa
    11461 agagaacaaa ttggttttgg atattaaaga ttgggcaaat ggaatttatt ttattaaaac
    11521 gcctactact tcacaatcat ttattaaaga ataaaaaaac ggtgttttat aagtgtcaaa
    11581 ctgctgcgtt gctttcaata ttcggactca gtcgcgtaca cttcggcttt gctcagtgca
    11641 acgcttcatg tacgctcctt cgtcctcaat ttcagacgcc ttgcatttta acacttctga
    11701 aacaccttga aatcacgttg caaaatagtg cttaaaacaa gaaaaaacgg ctactcaaaa
    11761 ggtagccgtt ttttttcata tttaatttaa ttatttcttt ttatagctaa tcttcacagg
    11821 agttggattt tgtaaattat cacttatagg acatctgctt tcaatagctt cgagccattt
    11881 ttccaaagta gcttcatcag catcagtatc tgctttgata cttaggttaa tttgtttaaa
    11941 accagcacgt tcttcattag actgtccaaa tagttttgca ggattcaaat ctccatagat
    12001 atcgatttct acacctctta actcaaaatt catttccttt gctactaaat gagctacaac
    12061 atttatacag ccagaaaaag ctgctataag atattcaact ggacttgcac cctcatcggt
    12121 tccacccatt tcttgaggtt cgtcaatgat aatttcaaaa tttcttgctt ttacaattgt
    12181 tttcattgga ctagcgctta ctgctttaat ccttgagctt aaatctgtca taatatttta
    12241 atttaaaagt ttataatatt tatttgtttc aaatatagaa ttgcaatacc tttttgcaaa
    12301 attaaaaaca cattaatatt tcataaacaa acatgcaatt ttacttgttt taatatctat
    12361 taatacttaa ttttacgtaa attttgaaat tttatatatg caagaaataa aaagattttc
    12421 atgggcaaca aatcttaccg aggaagaata ttctgaatta attaaaagtt caagtttaat
    12481 tagctacaac aaaaacgaaa cgatagtaaa acaaggcagt ttagcaagcc aaatcttgat
    12541 gttagaagaa ggttttgtga agctaaacat tgaaattaaa ggaaaagact ctacatttag
    12601 atttagtgga gatggcgatt ttattggctt ggcatgttca tttgtaaaga aaaaattaga
    12661 cttttctgct gtagctgtaa caacatcaaa agttagagtt ttcgacagaa atattttcga
    12721 agacttaata caaaaaaacg gaaaatttgc catagattta attactataa tgtcggaaat
    12781 gacaagcgct gccatacaca gtttaataat gattagccac aggaatgcaa atggtgcttt
    12841 agcaatttta ttattgcgtt tatacacact ttttaaatca gaaaagttta ctttaccttt
    12901 cactcgcgaa gaaatggcaa tatcattagc ttactcaaaa gaaagtgtaa tcaacattct
    12961 atctgatttt caaaaagatg gaattataaa acttagcgga agaaatattg aaattttaaa
    13021 attagattca ctaaaaataa ttgctgaaaa cggataagaa aatcaacaca aaaaactatt
    13081 ttcaatgaaa aaaatattca attttatatt agttgcagtt tttacattta cagcttgtaa
    13141 ttcgacaaaa agtcctataa aagttaaaaa tggtgtatta cagcctattg aaacagaagt
    13201 tcctgttcgt cctgctgggc aaaaacacgt agttggactt acagctccaa aaattgacgt
    13261 tgttcgcgtt ggttttatag gtttaggaat gcgtggacca agtgcagtat atcgttttac
    13321 tcatattcca ggagttgaaa ttaaagcact ttgcgacctt caccctgata gagttgaaaa
    13381 cactcaaaaa atattagacg aaagaggttt accacgtgct acaggctatt caggaagtga
    13441 agaagcttgg aaagcaatgt gtgaacgtga tgacattgat ttaatctata ttgtaactga
    13501 ctggaaacgt catgcaaata tggctatcta cgcgatggag catggaaagc atgtagcggt
    13561 tgaagttcct ggtgctatga ctttagatga tatttggaga ataataaata cagctgaaag
    13621 aactcaaaaa cattgtataa tgcttgaaaa ctgcgtgtat gactactttg aactaactac
    13681 acttaatatg cttcaaaaag gtgtgttcgg cgaagtgctt taggctgaag gtgcttatat
    13741 tcatcagttg gaagaatttt ggggtgctta cgaatctgac tggcgtttgg actacaaccg
    13801 caaatttcgc ggagatgttt atcctactca cggcatggga ccagcttgtc aagcgttaga
    13861 cattcatcgt ggcgataaaa tgaactactt ggtagctatg gacagtaaac ctgtaagcat
    13921 tcctgcattt attgagaaaa aaacaggcga aaaagttaca gattttaaaa acggacagct
    13981 tactatgact atgattcgta ctgaaaaagg caaaactata catattcaac acgacgtagc
    14041 aactccaaga ccgtattctc gcttgtataa tatagctggg acaaaaggtt acgcctctaa
    14101 atatccatcg ccaaactacg cgatacaagg agagcaatta gattcaagtg ttactccaaa
    14161 tcacgaagat ttgaatgctc actctttcgt ttcaaaagaa gttagagaaa acctgatgaa
    14221 taaatacaaa caccctatac acattgagtt ggaagaacaa gcaaaaaaag ttggcggaca
    14281 tggtggtatg gactttatca tggattaccg cctagtttac tgcttgcaaa acggtcttcc
    14341 tctagatatg gacgtttatg acttagctga atggagttgc ttagtaccgc tcacacaaat
    14401 ttctttggaa aataattctg caccagtcgc catacctgac ttcacccgtg gttattggaa
    14461 tatcacagaa ggctatcgcc acgcttttgc aaaataaaaa aagaaaataa aatgttcttt
    14521 ctctcagctt ctttcaactt taccttcggt gagctcgcaa gctcggttct acacttcaac
    14581 tacgttcagt gcatcgcttt taacttttaa ctgaatcagt aacttcccca aaatttcctc
    14641 aagccccact ctacggcagc aaaaagaagt atcacaaaaa acaataaata caaatctgta
    14701 aaatcgtgta atttttcatt tttataagct acatctgcaa tgttttgatc tgcttctatt
    14761 tgttctcgga ttttattcat ttcattaggg taaaaaacct tagcatttgt tttttcagcc
    14821 aactgatgca agacagaatg attagctgtt gtaatcaaag attctatatt aatacttttc
    14881 actagaaatt gcccagcaaa ttcataaatt ctattgtcaa attttgtttc aacattaaat
    14941 ttatattttc ctgcttcaag tcttcccata tcgagcctat aggagtttcc aaaattcaaa
    15001 aatgaagaag taaattcttt accttcttca tttgtcaaca caaggctaag atctaaattt
    15061 ggcactaaat caaaggcttc gttatagaat gacgcattta tcaaaacagg ttcgttttcg
    15121 ttatatattt gtttaaactc tacgttcagt ttatctttaa ttcgtttaac caaaagatat
    15181 tgcacagttt tatttataaa agcattgaaa gattcatggt cggaattata tttataatca
    15241 tcaagtcgcc atctccaaat tccttcgcca catataacac caagttttga gcctaaattg
    15301 tcgtcaacaa atgcaattaa tggtttagta gttttaacgc cattaataga ttgatacaaa
    15361 agaattttag cttcattttc aaaattataa tctgcaaaat tcacttttaa tggcgataaa
    15421 tatttgaaaa aactttggtc aacacctaac tcaaaggcaa caaaattcaa atttaaactt
    15481 ccaagtgcat cgtccatttt gtttgaattg gcaacaattt tcattcctgt ttcaacagag
    15541 ttaaacgctt gaattgaagt atttgctcca ataatataca aacttggaat agaatttttc
    15601 ttaatttctg aaagtattgt tgttgcagca aactgaacag atggcaactg atgtaaaatt
    15661 actaaatcat aatttttcac actttcatta aaatcgtaaa tataccttaa atcaagttca
    15721 aaatcgggat tatttttaag agcatattgt atagctccta aatcggggtg tggcgagttt
    15781 gccaagagca aaattttatt tctattgtca acaatattta tgataaagtc tgcaaaatta
    15841 ttttcgtagc ttatttctga atctaatttt tttaattcta cagaatattg ttgagttcca
    15901 actttatcag caaaaagctc tatgtcaata tatttttgct ttgagttttc aggtagttcg
    15961 aacttttctt tataaataat tttctcatct ttagtaacaa ttatttcgct ttctttttct
    16021 aaacattttt cggcagaaat taaaattcta actggaaatt tatttcctaa aaatgcaata
    16081 tgattgtgca ttatatcttt taacatcaaa tctttttgag aaatagtatc tcctaaagca
    16141 atagaataaa cagggaaatt gatattgtta agataaactg gattttcgcc actattaaaa
    16201 atcccatcag ttgcaattaa cacagctcct aaattcattc ctgcataact actaataatc
    16261 tctggaaaaa tacttgaaat attagtaagt tgtcctttaa aatttaaaac ggtatctgta
    16321 tttattgctt catcaaactg caaatatctt aaatcaaatc tattttttaa atctgcaagt
    16381 agcttattaa gttctgtttt atattgattt ttataataaa cagaatcttt atttagtaaa
    16441 attgacgcag aattgtcttg cacaacagct aaaattggct tttgaacaat agtttctgaa
    16501 cttttaaaaa taggatttaa gagtaataat aaaattatca gcacaaaaac aaaccttagg
    16561 cttgccatta aaatcttttt ccattttgca agttctttgt aaagtttgtc tttgtaatat
    16621 aataaaaaag aagtagccgc tgcaagagca acagctacta agattaaata ttttggatat
    16681 tcaaaataaa tttgcacttt gtaattaggt tagcattgct ccacaaacgt taatcacttg
    16741 tcctgatata tatgaagaca tatcagagcc taagaaaagt acaagatttg caatatcttc
    16801 gggttgtcca gcgcgtctca atggaatttt ttcagcccaa gcttgacgag cttcttctgg
    16861 aattgctttt gtcatttcag tttcgataaa accaggagct atagcgttta ctctaatatt
    16921 acgaggacct aattccttag caatagattt tgtaaatcca ataagtccag ctttagaagc
    16981 agaataatta gcttgtcctg catttcctga aacgccaact acagagctca tatttataat
    17041 tgagccactg cgttgtttta gcatagtttt ttgaactgtt tttgttaaat tataaaccga
    17101 ttttaagtta acattaataa ccaaatccca ttgttcttcg ctcattctca tcaataaagc
    17161 atcgcgtgta atacctgcat tattaactaa cacatcaatt ctttcaaaat ctgcaactac
    17221 ttcattaaca gttttttcag tagaattaaa gtcagcagca ttagaaacat agcctttagc
    17281 tttaactcca aatgaggtta attctttttc taaactttta aaattatcat cagcattcat
    17341 atctgtaaat gctacatttg caccagcttt tccacatgct aaggcaatag ctctgcctat
    17401 tccgcgtgct gcgccagtaa ttaaaacaac ttttccgtct aataatttca tattttgtaa
    17461 attttaattt atgcaaaatt acaataatat aaattaagaa caactattaa acaaaaatca
    17521 tattgatttt ttacgacttt tcttaattta tatttgtata gttttataaa attttactta
    17581 aactttctaa atttttgaca gtataaattt tatcgagtaa cagcttccag ccatctttta
    17641 agcgacttcc gcctataata ttttttgaca taggaaaagc ctcttccaaa acttcaaaat
    17701 atttgagcat ttttgcacta gtaatatttt cattaaattc tgtataaaca acatctggct
    17761 gaaaaacacc tttcattttt tctaaacttt ctattgtcaa cttgttaaaa taaaagtgaa
    17821 ttttatattt tttcactgtt gctaaaaaat aaactaaagc taaattacat gggaaagctg
    17881 atgaatcaga ctgaaatatc aaaatctctt ttgagcttct aaaggttttt tccttttcag
    17941 ctaaacacaa tatttttaaa gttaatttat taaaaaaata atccgcataa attgaatcaa
    18001 tattccgaat ttgtatcaac ttttttattt taaaaattaa tggttctaaa acattaacaa
    18061 taaattcttc gggaaataat tgttcataaa ctttatcaaa caaattttca aaaccttcgc
    18121 catctttttc taaaaagaaa aataaaaatt ctggcaaata tttattagac gaattaaact
    18181 ttaaactgta ttcttcaaac aagttttcaa attctgcttc ggacaaagaa cttaaataac
    18241 taattttaaa cttaaagctt tgtaaaacag taattttcaa caacaagttc aaatcttttt
    18301 gggaataaat tctttcattt tgcacagaac gtaaaggaga aaaaagttca tagcgtttct
    18361 cccacatacg aattgttgat gcttttactt cgctaataag ttctaagtct cctatgctat
    18421 atgtttgaaa taattccaat aaaaaagatt ttgtttgtca atttgctata caaaaataca
    18481 attatttttc aaatgtaagc aaaaagtata atttttttca ctatttgacg aaaaatattg
    18541 tcaattttat ttattgacga gtttaatttg agtattattc atttttacaa tcacatttgt
    18601 tttggataaa atattccaat tgattaaaaa aatatttttg ttccattata tctaaatctt
    18661 taagtgtatc aattagttgt atcggttttg aaaaatataa attacagcaa gtatcacata
    18721 aatattcaat tgtcttatcg ctgctatcaa atatttccaa attatgattt tgagaatatt
    18781 catttaacat attataaata ttattagtaa gtaatttttt acattgtggc aataataaaa
    18841 tttcatcttc tttacgtttc tcttttttaa tttctaactc atttatatct ctacttttaa
    18901 caactatttc attaatttct ttttcaaaaa acaactgagg attaataaaa aagtaaaatt
    18961 ccataccgtg attttcaatt attgaaaaat tcccaagcat caaattaaaa tccccaaaat
    19021 ttgtcatgca cccatcaatt tcataacgtt ttttattaaa ttttattttt cctttccctt
    19081 taattttatc taaaacaaga ggaattttaa tttttccttt ataattactt ggcataatta
    19141 attttgcaca taagaaaaaa ttaccacgct cgctattttt ccacggtata atcaattctg
    19201 ctccctctgg taaaaaataa atattttcat tttcaagttt ataatcaaaa ttggtgtaaa
    19261 aaactctatt atcttctaga gtagaaaaaa gtagtgcatt attcattaca aaagccattg
    19321 taaaagtcat cttaggacta tctatcacaa catatcttgc tgtatcatta aaccattgtg
    19381 gattatcaca caattcttta tatgaggcta tttgtttatc atcgttcata aaatacacgc
    19441 ctaaatcagg atcgaggact ttccacttac catctgaaaa aacttctgtt acaacatgtc
    19501 cttcaataca ccagctacga gctttgtaac ccaatgctac aaggatattt gttaaaacag
    19561 cagatcttgt tccacaaagt cctcctccaa cagagcttaa aataagttct ggcatagtag
    19621 ttcgtgtcca atcatcaata acaatatctt tatcatgaaa cgtattatta taaacaaatt
    19681 cccaagcaca aatttctaaa ggtttttcag gaaattcttt agctttgtct ttaatataac
    19741 taacaatatt gtccaaactt gaaaaatcct tgttgtttgg taacacctta aattcaatag
    19801 gcgaacgtcc tgcattataa atagaaatat attcatctaa attatatgag attttaaatt
    19861 cctcttcttt tttattgata ttactacacg aacatactaa ataaacaata caaaccattt
    19921 gaattagctt aacaagaata agttttattg aaaaattttt caaactacta aaaaaaacaa
    19981 ttttatgata atgcataact cttaaaaatt taatatatca caaagttata aaataactgc
    20041 aaaaacaaga tttttctcaa aatgaaaaag cctactcaat tcagagcagg ctttttcgat
    20101 acttattatg aaaaaaaaaa tttacttagt ttcttcgtcg tcttttttag ctttagctgc
    20161 tgtttgcttt gcaggttttt cttctgtttc ggctttagct ttagcctctg tttttgcctt
    20221 tggagctgct tttgctgtag tgtctgcttt tgcttttgga gtagcctttg cttttggttc
    20281 agtttttggt tcaacttcgg cttttacctt tggagcagtt ttagctgttg tttctttctt
    20341 tacaggtttt tcttctacaa caacttcttt ttcaacttta acctcttttg ctacaggttt
    20401 ttcgtcagca acaggttgag gcttagctac tttttttgca ggtttttctg caaccattgt
    20461 ttcaactgtt tcaaaaggaa gaacagagac ataagatctg ttatttcttt tcttctcgaa
    20521 ctttactcta ccatcaataa gcgcaaacaa agtatgatct ttacccatac ctacattatt
    20581 gcctggatga tgttgagtgc cacgttgacg aactataata tttccagcga tggcagtttg
    20641 cccgccatat attttaactc ctaatctttt gctttcggat tcacgaccat tacgcgaact
    20701 accggctcct ttcttatgtg ccatttctaa ttactttttt aaattatgct ttaatgtcct
    20761 gaatttctat ttcagttaaa tactgacgat gtccatttaa agtttgataa ccttttctgc
    20821 gttttttctt gaatacaaga actttgtcgc tcttacagtg tctgagaact tttgctgaaa
    20881 cttgagcttt ttctacaaca ggtgcaccaa ctgtaacttt attattatca tctaataata
    20941 atactttatc aaatgttact tcgctacctt cttcgttttc taagcgatgt acaaaaaatt
    21001 tgtttccttt ctcgactttg aactgctgtc ctgcgatttc tactattgcg tacattgttt
    21061 attcaattaa atatatttct aaattttaaa aaatcggact gcaaagatag aaattattat
    21121 tctattaaca aactattttt cattattttt tttataagtt ttaattttct cgcaatacca
    21181 agggtttaaa cttaaaaaat cacatatttt cagtagtttt aaataaaaaa atctgaatta
    21241 aatactaaaa cttccaaaac tcagttatat ctttgcacaa aatttgattt tgagaaattt
    21301 tattttttac atatcggttt taagtttttt attgcctata aatttaagtt tggcacaaaa
    21361 accaattcca atgaacttac caaaattaga tcagcaagtt gctcattttg gatattcttt
    21421 gggcttaaac actatggact ttaatattag accttcagca aactttcttt cagaatttga
    21481 cactgtaatg gcagttgaag ttgaaagatt tgttggtttt aatattaata ttatttcaaa
    21541 tctaagactt gccgaacatc ttgatcttag atttttacca ggattagttt tcgggcaaag
    21601 aaacttagaa tataaattta taaaaaacaa tcaatttagg cgacacacta tgatgataga
    21661 atcaactttt gttgatttac caatatgtct taaatatcgt gcagcgagaa ttaacaattg
    21721 gcgtccctat gttttagctg gctcaagtgt tagaattgat ttggcttcac aacgtaaaat
    21781 aaatcccgaa gaaagaccaa aaattagact taaacctttc gacacctatt atgaagttgg
    21841 tgttgggcta gattttttct tagagtattt tatgtttgga atagaactaa aatcttcgtg
    21901 gggaatattc aatatagttg cttacgacaa aacacaatat tcacagtatt ataaaaaatt
    21961 aaattctcat atggtaattt taggctttca tttcgaaggt ggtaaaattg ataaaattaa
    22021 gtggtggaaa taagcaatac agttaaaagt taaaagaggg aaattaaaag tctgaaattc
    22081 aataaattat acagcatagt gtagagacag tgcatgcgcc gtctcttatc tatacaaatt
    22141 ataatttatt gtactatcct aattctgtgc cgaacaatta cgccttgccc gcaagggcaa
    22201 catgtgatta accgtaagat ttatcttgcg gcgaaaacac tcccccagac cctttgcccg
    22261 caagggcaac acaataaaaa cagttaaaag ttgaaccgag tttacgagct cgctgaaagc
    22321 aaaagtttct aaagttacaa agtttgcaaa atttctaaag tctctaagtt gttgtctggt
    22381 agccaaatgg tattaatgcc gagttgtttt gcagcttcaa tatttgcttt attatcatca
    22441 ataaataatg tttcgcttgc ttttagttta gaatcgttta atacaaattc aaaagcttgc
    22501 aaatctggtt ttctaaaacc tattgtgtga gaatagtatg ctcgttcaaa aatttcatca
    22561 aacgatgcga agccaaattc ttttacaaaa tttgcattat aatattcgta atgaattgca
    22621 tttgtattac ttagcaaaaa tattctgtat ttatttttca acgataataa aagttctatt
    22681 cgttttggtg gaaaatctaa aataagtgca ttccaacaag tttctataat tttatcatct
    22741 aaatctgtat ttgaaatttg cctaagttga gaataaaatt cttgtggact aatttttcct
    22801 gtttctaaat aatcgaaaat ttttgtttga ctaacaagac tataaagttg atcaaaattt
    22861 tctaaaccat gctttttaaa ttctatttcg cttttattta tgtcaatatt taaaataaca
    22921 tttcctaaat caaatattat attttttatt atcataaagt cttattgtct attttttaga
    22981 ttttcgtttc caaaacgatg ttcttttctt tacttttggt tcttcaagtt ttacaatgtg
    23041 aggcgagtct gggataagaa tttctctaat tttttgccat cctagaaaac ctccaatatt
    23101 tttatcgtca ataaagatat caacatctac ttttctacta atatacttat taaactcttc
    23161 gtttggataa ctttcattta ttgcccaaaa aatcacacca ttttcaagac aaaaattaac
    23221 agcattttgc aaatctatac catctctcac tgtccacaaa attaatttat gtccctgagc
    23281 ttgaagttca cgtaaaactt caaaagaacc cggtataaca tttcctatat caggatattt
    23341 gtgttccaca atggtaccat caaaatctac tgcaattttc aattccattt tttgtgttta
    23401 atttgctagt ttacttgctt ggtttatata aattatagtt gcgccaatca ttttacctta
    23461 tacatatata cttttgcatt tactgcattt ggatcttctg gtgctataat tagtttatct
    23521 tccgtaagag tgattatctt agttctatct tccttatctc ttccttttat ctttacgcta
    23581 acatattgtc catctttaga aattctccaa gtgccttcag ttgtaccatc aaaacctttc
    23641 tttaaatatt ttttgttagg ataaaacagt acttctgtag tttcaataat ttgtgccatt
    23701 ttttcttcta taatcggctt gtattgaatt gctaatggac tatcccccac atctaaagaa
    23761 tccactttcc actttccgat aatcatttta ctattattcg ctcctaagtt ttgagcatac
    23821 gagtttatag aaaagataaa cattaaacct agtaataaaa aaagtcttgt cttcataaaa
    23881 aaggtattat ttgtttgtaa taatttcatt tgttgacaaa tttaatactt tataagatat
    23941 atccaaattt ttcaaaatat ctggcattct tgaataacta gtgtcggtaa tgaaaatttg
    24001 cccaaactca ttatctgcaa ccaattcagt aattacctta actctttttt tatcaagttt
    24061 gtcaaatata tcatctaaaa gcaaaattgg tgccatatcc aacttgttac gaatatactc
    24121 aaaccaagca aatttaagac taatgacata tgttttttgc tgaccttgag agccgtatct
    24181 ttttataggc tgattttcga ttataaaatc aaaatcgtct ctatgaattc cgtaagaagt
    24241 atatcccaaa gccaaatcac gttctaagtt ttttctcaaa ctatcatata aattattttt
    24301 atgaagttga gatgaataaa ccaattctac tttttcatta ttatcagaaa ttaatgtgta
    24361 atatttttga aaaacaggaa ttaattcttc tgcaaattgt tttctcacct cataaatttt
    24421 ctctgcattt tcggaaagtt taaaatccca aatttcaatt tcatcaggtt ctattctttt
    24481 tgtagaaaag ctgtttttta acaaaatatt tcgttgctca agtactttgt tgtaatttat
    24541 aagattattt aaatatgatt tgtcaaattg agaaattatt gaatctgcaa actttcttct
    24601 aatatcactt ccttgatgga ttaaatcaga atcgtatgga ctggagcata ctactggaag
    24661 taaacctata tgttctgaaa gtttttgata ttcaatacca tttcggctaa aagatttgcg
    24721 tttatctttt tggacgccac aatatatatt ttcaacattg tcatcaagct caaacttgcc
    24781 ttttataacg aaaaaatctt cgccagtttt tatattttgc ctatccgatg ggtttaaaaa
    24841 acttttacaa aaagataaat aatgaatgct atcaagaagg ttagtttttc caattccatt
    24901 atcaccaatt aaacaattta ttttagcaca aggctctaat tcaaaacttt caaagttttt
    24961 aaaattaatt agtgataaat tctttaagtg catattcatt tttacaaaag cacaaattta
    25021 ataaaaatgt gaagatttat aaatttaatt ttgtaaatat ttttaacatg tttattgagc
    25081 gttaaggatt cttacctatt tattaatata tcaaaaaaac tttatttcat tgtaccaata
    25141 aattaatatt tgtgtataga ttattttcat tcaaagtgct ttttcaattc gcttgattga
    25201 aaaaacaatt ccatttcaca cattgctttt ttgggatttt tcttgtcata atttccaagt
    25261 gcaatagagt tttcaacagc tatttttaat cttggtttta actttaagta aatatcgtct
    25321 ccttgtttag tgaaaaactt tcgcgttttc tcatagtttt ttaagtattt ttttaattca
    25381 gtttttgctt ctgcacaagt gtcataatac tttgatgttt ttttaaaatg aagcagaaac
    25441 caaaattcaa gacaaggatt atttacaatc acaacaacgt ttgcataatt ttcttctaaa
    25501 tttgctctat attttgcaaa aatttcaagt ggtgtttctt tcccttttag tgcttcgtta
    25561 tcttctttga taacagaatc aaggtcaact attcaaaaaa cttttgtaaa ttccttttcc
    25621 gacaaatcac gaactaaatt gtattgttct tctacacttt ttttatttgg aatttcgggc
    25681 tttatgctaa tacgaatttt tggttcgttt cttttcaaca tttgcaaata ccaaacttcg
    25741 tcatcgccgt caactacaac agcaatagat gttggtggtt taggtgcata tcttttaggc
    25801 tttctcatag tttcacatta aagtcaatga aagtatcgcc caaattaggt gtagcactta
    25861 attttccact tttataggcg tttaaaacat ttgtagtatt acgaacgaca gagctgtcaa
    25921 aatcagccaa agaataaagt tcagttgagc aattttcttg tttatcagta aaccatatta
    25981 catcatttct aaaaacatct ctgttatcta aaatctctct attatgtgtt gttgcaatga
    26041 tttgagaatt ttcagaattc aaaagaaaag aaataagaaa atgcaaatac aaatcagggt
    26101 gtaaagaagc ctctaattcg tcaattagaa atgctgttga attttgaatt agcaaagcta
    26161 acaaaccagc aaacccatag taacgccttg tcccttctga ttcttcatct aaaggtaatg
    26221 tgtatttgac attattgact tcgtgttcaa attcaacatt aatagctgtt aattttcctt
    26281 tttcttccaa ctctctagct ttatcactcg gaacatttac ttgatttttt aaaaactcaa
    26341 taaaagcacc cggaaaatcc ttttcttctt tttgaattac aatatcagaa atatttaagt
    26401 ccgctttttt taaaatactg accacatctg atttagaaat tttcttatta tcaatttgcg
    26461 aagtaacaaa accttctaat tgagttctgg cataaattaa tgggttcaaa tatttaacaa
    26521 accagtctat aacttcttgt aattccttaa aatcaatatt tgtttttaga tacccaccta
    26581 gaacagtatt attccaaaga gtattggctt cgagagtctt cttaaaaact ttatctgcag
    26641 aaattttact tccaaaagtt atttctgtaa actgcttttt taaatctgtt tttcgtttaa
    26701 aaatattagc tttattagga ttatgaaaat acagcgactc tgaaacaatg gcttttttac
    26761 aaaattctac ttcataaaaa tatttaattt cattttgaaa aaactctaac gaaataattg
    26821 agttttgttt tggtgtaatc gagtcaaata gaaaagggct aaagtctaat tctgtagttt
    26881 tcttatcttc tggctcaaga actatttgtc gcaaaaaatc aagtgcttta agtattgttg
    26941 tttttcctga tgggtttgcc ccataaatta atgcgagttt taaaatccgt tgtcctccta
    27001 tattagcgac ataagcgtct tcaaggtggg ttgaattatc tgcctcaaaa gaaagagttt
    27061 gttcatcttt tattggacca aaattttgaa cattgaaatt aagaatcatt ttttacactc
    27121 gttttgtaat tgttttgcaa atttacaaat aaaactaaca caaaagcaaa ttttttgctt
    27181 aatttttaca atccgcttac aaattctcgc ctaattatat tttgcagatt gttttaagta
    27241 aatattttta taattttgcg tgattttatg attaattcat tgaaaatatt aaagcaaaat
    27301 aatctgagtt ttacagacaa tattaaataa ttatgacttc agaaatttta ttcatcatca
    27361 ttataacaat tgtagtttta gattttttcg tagaacaaat tttagaatat ttaaatgctt
    27421 cgtggtttaa taaacctatt cctgaaattt tgaaagacat ttacgacacc gaaaaatata
    27481 aaaaacaaca aagttataaa tcagaaaatt atcgctttgg gattataagt tcaagtttta
    27541 gttttatttt aatagttcta atgcttgttt ttaatggatt tgcctatgtt gacactcttg
    27601 ctggtcaaat ttctgaaaat tttattctaa aacctctagt gttttttgga attattttgc
    27661 ttgctatgga tattttaagt actcccttta gtgtttacga tacttttgta attgaaaaca
    27721 aatatggctt taataaaacc actgtaaaaa catatatttt agacaaaatt aaaacttatt
    27781 tgctaggcgg aactattggt gctggcttat ttgctttaat tttgtggatt taccatttag
    27841 accctgaata tttttggcta tatgcttgct tggtaattat tgctttttcc ttatttatga
    27901 atatgtttta ctcaactctg atagttcctt tatttaataa gcaaacaccg cttgaagacg
    27961 gcgaacttaa aacaaaaatt atggaattta gcaaaaaagc aggtttcaat atttcaaaaa
    28021 tatttgttat tgatggttct aaacgctcaa caaaagcaaa tgcatatttt agtggtatag
    28081 gaccaaaaaa acgtattgtt ttgtacgaca ctttaattaa tgaaatgaca aatgatgaaa
    28141 ttcttgcagt tttggctcac gagattggac attacaagca caaacacatt tactggtcga
    28201 tgattgctgg cattatagaa ataggaatta tgttttatat ttttggactt tttgctagta
    28261 gcgacatgct ttctcaagca ttaggattag aaaaagcaag ttttcatatt gcacttatag
    28321 ctttctcaat tatttactct cctatttcaa gtgcttttgc agtagtttct tcaattttta
    28381 gtagaaaagc agaataccaa gcagacaatt ttgcatcaaa acatggacta tcaaacgaac
    28441 taatttctgc acttaaaaaa ctcagctctc ataatatgag taatttaact cctcaccctt
    28501 gggttgtatt tatgacttac tcacacccac ctgtttttaa tagaattaaa gctttaatga
    28561 aacattaaat attttcgcat ttactataaa caaaaatatt tttttcatta cctattaata
    28621 ttaatagaaa agtaactttt aactagatta tttcagttat gctcatttcg gctccgctca
    28681 atgagcaaaa ttgaacgctc aatgagcaaa attgaacgct caatgagcaa aattgaacgc
    28741 tcaatgagca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaattgaacg
    28861 ctaaatgagc aaaattgaac gctcattgag cgtagtcgaa atgagcgtac aaaaaaaaag
    28921 gctgtcatat aaacagcctt ttttactttt gttaacttta aatttacttt agtttttaat
    28981 aaatctttga gttccaacat tgccattttc gtctgttaat acaacagtat aaacgcctgt
    29041 agcaaattca gaaacattaa tttcagcatt attagaaact tctacatttc ttacaagttt
    29101 tccatctacg ctataaatat ttaagataga tttttgtgat tgagtttcaa tttttaatat
    29161 atcttgtgtt ggatttggat aaactttaaa gttagctaca acatcttctt taacataatt
    29221 gtcatgagga agatttatgc gaattgcaaa tgctttgcca tcagaagcac cataatacca
    29281 tccccattct ccaccgtaca atattctgta ccaaccagac caagcaccaa aattagttga
    29341 tttatccata ccaatatcaa tggtgttttt tgaataagaa cctgtataga acattttcac
    29401 agccacaaaa acttcgccaa caccttcatt aacaagatca acgataattt ctgtattatc
    29461 tgcaaaagtg aaattaaccc atgtatttaa atgctctgct gctaaaatta atggttctga
    29521 ctctgcaaaa gccacatatt cttcaccgcc ccaacttaac aaaacaagtt gaacagtatt
    29581 acctattgtt ccccaatttt cagcatcagg atctccttca atataaacat caacggattc
    29641 aattgttgtt gtttcgttta taaaatattt tgtagcaagc atttcttcat tattaccacc
    29701 aatattccaa gcagctaaac cataagattg agttatattg tccaagtccc ttgcatatct
    29761 accatctcct gtaacaaaaa atcttgtttc atataaattg ttatttggat tttgatcttc
    29821 ttgtccttga atagtaattt caatagaagc tacatattct cctgttacaa taggtgcttc
    29881 aagcccaaat tcaggtgcca taatatcaat agtatcaaca gctccagaag ctatttcagg
    29941 acctgtaata gtgttattat aaacaactgt tccctcagga tttgtaactt caactttaac
    30001 aataggagtt gctacttgca aaccgttatt ttctactata gtattaaaaa acatataaga
    30061 agcttctgta tccatttgtt cttttggtat ttgtccaaaa tgtccagaat agtggtatcc
    30121 gttagcattt ccagcctcat gataatctat atatacaaaa aagttcatag ctgtttcaag
    30181 atgttttaaa tcatattgtg gagcttcaat aaccataaca tcatcaattc cccatgcata
    30241 gccagttgca tatatttcat aacctgttct atcatgagtc caacggaatc taatagaaac
    30301 attagattca tttgctgcta ttcctaaaag taaagcttgt tcatattctg catattcacc
    30361 atgtgcaata tcggcacttt tattaatttg atatgatgtc caagtagcac catcaacaga
    30421 aacatcaaca tataattttt gcatactagc gttaaaagtt ctataatatt ggttccaaag
    30481 tagcatagga ttattaacag tacttaaatc aatatcatca aactgtatat aagcttcaaa
    30541 cacaacaggc atattagcaa ctgagtctgg tctatccgct gtagctgcac gtagaatagg
    30601 accataaata tcaatgaaag cccatttgtt agcatctgtt gcagagtctg ccatagggaa
    30661 caaataccaa ccattttcaa aatcacccca acctgctgcc ataaattgtt caacatttac
    30721 tgtttgccat ttgtattgac caactacagg attagcataa gtagtccaag cttcagctcc
    30781 gtcaaaatct tgagtccaag ctacattagc ttttaaagct tgtctttcca tatacttttc
    30841 aaccatcgtc ctagtatcct tttctatgtt tttacaagca atgttactag gtgcttgtat
    30901 agcctgagcc attgcaaacg atgcaagagc aacaaaagcc attaaaattg taaatttttt
    30961 cataatttta aaatttaaat taatacttat tttattttct tattcattac aatattaaaa
    31021 aatatcaatt acaaaaataa tgtttttttt ttatatacaa aaatttttat gctttttttt
    31081 caaaaaaaac tcgaaaactt taacaaaact cgcataagcg ctagtttttg ccaataaaaa
    31141 tcctcgtttc ccatctagaa aaccaagttt tataatatac atagatataa atttataaat
    31201 tggctttaaa taaagcataa gatatccata attttttcca ttttcaaaat ctttttgtgc
    31261 cattaatttt gcatatttaa tagttttttg cttatgaatt tctttgcttt cgtaagtaaa
    31321 atgcaacaaa tttcctttta aaaaacattt ttttggaggc tgagaaaaca caattttctc
    31381 gtgtattttc ccttcccatt ttccataatt tttattccaa attctatatt ttttatcggg
    31441 ataccaacca ccaaacctaa ttggttttcc acaataatga tttaaaagat taaacccata
    31501 aaattcattt tctccaaatt caattttttc aatagacgaa cgtaattttt cagaaataac
    31561 ctcatctgca tctatagata atatataatc atattttgca aaagagttac cataattttt
    31621 ttgagagcta taatcatcaa aatctcttac aaaaaattta acattatatt gtttacaaat
    31681 ctcttctgtt ttatctgttg aacctgaatc aacaacaaca atgtcatcgg ttaaagtttg
    31741 taaagaatgt aaacatttgc ctatgttttt ttcttcgtta agagtaatta taactgcact
    31801 tagttttagc attttttact tcaaaaattt tattttacaa aaataataat ctaaataaaa
    31861 taaaaaaaat ttacatttat ataattaaag ctaagcacat attttttatg ccatttgctc
    31921 aaaaatttcg accttttgaa ctttaaaact ttcagctgtc gtgtcctata gacaaacttt
    31981 tgataaagtg atgaaagtta atttgccact gccaacaccc tagatgctcg ctcaaacacc
    32041 ccagatgctc gctcaaacac cccagatgct cgctcggatt tgcaatccga gtgcatggta
    32101 atgttacact cggattgcaa atccgagcga gcggggagga ttgcaaatcc gagcgagcgg
    32161 ggaggattgc aaatccgagc gagcggggag gattnnnnnn nnnnnnnnnn nnnnnnnnnn
    32221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32281 nnnnnnnnnn nnnncaaatc cgagcgagcg gggaggattg caaatccgag cgagcgggga
    32341 ggattacaaa tccgagcgag cggggaggat tgcaaatccg agcgagcggg gaggattaca
    32401 aatccgagcg agcggagaaa ataaaaaatt ttacatttat ataattaaag ctaagcatat
    32461 attttttatg ctatttgctc aaaaaattcg actttatgaa ctttaaaaac tttactaact
    32521 ttaaaaactt attttgtaat ttaaacaaca taaaaaaaaa tttttttttt acatctatta
    32581 taagtatttt tggaaaaatt ttaaaaaaac ataaaaatac gaaattatga ataaaattat
    32641 cactgttgaa gaagccgtta aaaagataaa tgatggtatg acaataatga taggtggttt
    32701 tttagctgct ggttctcccc atacaataat taaaggatta gttgaaaaaa acgtaaaaaa
    32761 cttaactctc attgttaacg acactggttt tgcagataaa ggaattggtt tattaatatc
    32821 caatcgtcaa gttaaaaaag taattgcttc gcatattggt acaaatactg ctactttaga
    32881 gcaatttaat aataaagaat tggaaattga atttgttcca caaggtactt tagcagaacg
    32941 tgttagatgt ggcggtgctg gtttgggtgg tgtgcttact ccaacaggtt taggtactgt
    33001 tgtagaagaa ggcaaacaag ttataaatgt aaatggcaaa gattatttac tagaaactgc
    33061 tttacgtgct gacgttgctc taattggagc aagcattgcc gatgaaagtg gaaacttgta
    33121 ttacaaaggt actactcaaa actttaatcc gcttatggca acagctgcag accttgtaat
    33181 tgctgagact aaagaaatgg taaaaatcgg cgaaattgca atggaaaatg ttcatactcc
    33241 tgctttattc gttgattata ttgtaaaatc ttaaaaaaaa gaaaatatga aaactacatc
    33301 tatgattaga ctaagaatgg gcacaaacga tgctcattat ggtggaaatt tagttgacgg
    33361 agcaaaaatg cttcaactat ttggagatgt tgcgactgaa ctattaatta ttaatgatgg
    33421 tgacgaaggt ttgtttgttg cttatgacaa tatagaattt ttagctcctg tttatgctgg
    33481 cgattttatc gaagcaactg gcgaaatagt tagccaagga aacagttcta gaaaaatggt
    33541 ttttgaagca aaaaaagtta tcaaacctcg cacagatatt agtgcttctg ctgctgactt
    33601 tttagaagaa cctatagtgg tttgtcgtgc tagcggaact tgtgttgttc caaaaaattg
    33661 tcaaagaaaa aataaataaa ttattatgga taaattaatt attacagcag caatatgtgg
    33721 tgcagaggtt acaaaagaac ataacccagc tgttccatat actgttgaag aaattgttag
    33781 agaagcaaaa tcggcggttg atgctggagc agctattgtt caccttcacg ttcgcgaaga
    33841 cgacggcact ccaactcaaa gcaaagcaag atttaaagaa aatatagacg ctattttaaa
    33901 agcatgtcca gaagtaattc ttattccatc aactggtgga gctgttggca tgtcgcctga
    33961 agaaagactt caaccaaccg aattgccaat agaaatggct actttagatt gtggcacatt
    34021 aaatttcggc gacgaagtgt ttgaaaatac tcttccaatg atgagagctt ttggaaaaag
    34081 aatgatagaa agaaatatta agccagaata cgaatgtttt gaaatgggac acgtagaagc
    34141 ggttcttgat atggttagaa aacaacaatc tcctggtgcc cctctacatt ttaactttgt
    34201 gcttggagtt ccaggtgcag caaaagcaac tatcaataac ttagtatgga tggtaaatgc
    34261 tattccacaa ggttcaactt ggtgtgctac cggagtagga agacacgagt ttcctttagc
    34321 agctcatgca attgcaatgg gtggacatgt aagagttggc ttcgaagata atctttacct
    34381 tgaaagagga gttttagcaa aatcaaatgg cgaattagtt gctaaagttg taagaatggc
    34441 taaagaatta ggacgtgaag ttgctaattt tgccgaagca agagaaattt taggtttaaa
    34501 acaaaaataa attttaatta atttttaaat atatgcaaaa aaaaggaaat aaatatggct
    34561 tacatagagt aattgagcca ataggagtat tgccacaacc agcaaaaaaa gtaaataata
    34621 atatggacga aatatgggat aacgaaatcc taattgatgt tcaaacttta aacgttgact
    34681 ctgcaagctt tactcaaatt aaagaacaag caggtggcga caaagccaaa attgcagaaa
    34741 taatgcttga tatagttgct aaacaaggta aacaccgcaa tcctgtaaca ggttcgggtg
    34801 gtatgttact tggaacagtt gaaaaaattg gagatgcact aaaagacaaa attgacctta
    34861 aagttggcga taaaattgca actttagttt ctctttcttt aactccactt agaattgata
    34921 aaattaaaga aatacgtcct gatattgacc aagttgacat agacggaaaa gcaattttat
    34981 ttgaaagtgg tatttacgca aaaattccta atgatatgcc agaaggttta gctctttctg
    35041 ctcttgacgt tgcaggcgct cctgctcaag ttgctaaatt agtaaaaccc ggagatacag
    35101 tattaattat aggtgctggc ggaaaatctg gaatgctttg ctgctacgaa gctaaaaaac
    35161 gtgcaggcgt aactggtaaa gttataggca taggacatag cgaaagaagt acaaaacgta
    35221 ttatggactt aggtctttgc gaccatgtat tttcagcaga tgcaacacaa ccagttccta
    35281 ttttagaaaa aattgaagaa cttactaaag gcgaaatgtg cgatgtaaca atcaataacg
    35341 tgaatattga agatactgaa atgacaagta ttctttgcac aaaaaatagc ggaacagttt
    35401 atttcttctc aatggcaaca tcatttacaa aagcagctct tggagcagaa ggtgttggaa
    35461 gtgatgttac aatgattgta ggaaacggct acaccaaagg tcatgctgaa attactttgc
    35521 aaattttaag agaaagcgaa aaactaagaa aagttttcaa cgaattgtac gcttaataga
    35581 tgataaaata ttaaaataca ataactttgg cacaaatcag tgtctaatat aagacataaa
    35641 aattaaaata aaatgaacga aggaagaaaa aaaatgtttc caaacgcaac agatgaacaa
    35701 tggaacgatt ggaaatggca ggtgaaaaac cgaatcgaaa ctttagacga gttaaaaaaa
    35761 catattaaac tcactaaaga agaagaagaa ggtgttgcca agtctttaaa aacattaaga
    35821 atggcaatca caccgtatta tttaagttta attgatccaa aagataaaaa ttgccctgta
    35881 cgcaggcaag caattccgac aggagctgaa actcatcagt ctgctgctga tttgcttgat
    35941 ccattaagcg aagatgaaga ttctcctgtt cctggtttaa cacaccgcta tcccgacaga
    36001 gttttatttt tgattactga tatgtgctca atgtattgca ggcattgcac tcgtcgtcgt
    36061 tttgctggac aaacagataa ggctagccca aacgatagaa ttcaacaagg tatcgactat
    36121 atcgcaagaa caccacaagt aagagacgtg cttctttctg gtggcgatgc tttaatggtt
    36181 tcagacaaaa tgcttgaatc tataattcaa cgtttaagag aaattcctca tgttgaaatt
    36241 attagaatag gaactagaac tcctgttgtt tgtcctcaaa gaattacaga cgatttggtg
    36301 aatatgctaa aaaaatatca tccaatttgg ctaaacactc attttaacca ctcaaaagaa
    36361 attactgcag aatctgctga agcttgtgct aaattagcag atgcaggaat tcctttagga
    36421 aatcaatccg ttttgttgcg tggcgttaac gattgcgttt acacaatgaa agaattagta
    36481 caaaaattgg ttaaaattcg tgttcgtcct tattacatat atcaatgcga cctttctatg
    36541 ggactagaac attttagaac tccagtttct aaaggaatag aaattattga acatttacgc
    36601 ggacatactt ctggttatgc tgtaccaact tttgttgttg atgcaccagg tggaggagga
    36661 aaaactcctg ttatgccaac ttatataatt tctcaaagtc caggcaaagt tgttcttaga
    36721 aattttgaag gtgttattac aacatacaca gaaccaacag attacaaaga agaatgttgt
    36781 tgcaaagatt gtaaagaaaa aactaaaaac gaaggtgttg cttcattatt aagaggcgaa
    36841 cagcttgcgt tagagcctaa ggagcttgct cgaaaaaaac gattctaaaa tataacggtg
    36901 tttcataagt gtcaaactgc tacattgagt taaaagttaa aagcgatgca ctgaacgtag
    36961 ttgaagtgtt aaaagcgatg cactgaacgt agttgaagtg tcgaacctag tttacgagct
    37021 caccgaaggt aaagcgatgc actgaacgta gttgaagtgt tgaaccgagc ttgcgagctc
    37081 accgaaggta aagtttgcat ttgaacattt ctgagacacc tttttttcga tatagtttgt
    37141 tttaaaataa ttattttcca agtattccac aaattcaaaa tttcttatta attttgggca
    37201 attattttta aacactaaaa attgcctttc ttaaaagaaa tattaaaata caaaagcctt
    37261 tctattatag gtacagaaaa aaacactggc aaaactgagt gtttaaatta tattattcaa
    37321 tctttaaaaa attcaaataa aacaattgca ataacatcta taggaataga tggagaaagt
    37381 gttgacgctg ttacagaaac tcctaagcct gaaatagaaa tttataaaaa caatatcttt
    37441 ttaagctctg aaaaacactt tttacaaaaa caaataacag ccgaaatttt agatgtttct
    37501 gaaaaacaaa cttcgctagg acgcttgatt acagctagag ctatggacac aggtaaatta
    37561 attttttctg gtccgcccga cacaaaatgg ttgaaagaag aaattcaaaa ctcacaaaaa
    37621 tatggagttg atttattctt agttgatgga gcattgtctc gtaaaagcac tgcctcacca
    37681 tcggttactg aaagcatgat tttaacaacc ggtgcagcac tttcggcaaa tattccaact
    37741 ttggtaaaaa agacaaaatt tatatgtgat ttaattgaac tgccagtttt tgaaaccaat
    37801 ttaacaaacg aacttttaca aataaaaaac ggtctttggg ctattgattc agataatatt
    37861 attcacgatt tagatataaa atccactcta ctacttgaca aagcaaaaga taaactttac
    37921 tcaaaaggtt gtgtgatttt tgctagcgga attattacta ataatatctt agagtttatg
    37981 agaattcaaa aaaacataaa agaaacaatt cttattgtaa aagattttac aaagattttt
    38041 gttagtcaag aaaaatataa ttcgtttaca aaagctggcg gaaagattat ggttttgtta
    38101 aaaccaaacc taatttctat ttgtgtaaac ccatggtctc caagaggaat tgtattagat
    38161 tctcaaaaac ttagagaaga actttttaaa gcaattaatt tacctattta cgatataaaa
    38221 gcacttgatt aatgaaaagt tttagaaaat atataagcga aattgcagga ttaagattcg
    38281 tttatgaaaa tcttgaacta aactcctctg cgggtcgaaa aaaactgctt aaatctcaac
    38341 tttttaccaa agagtttgaa ttaacagtaa atattcaagc tttaagcgaa atttatgatt
    38401 ttatacaaga taaaaaaaac aagaaaattg tttcagatat ttgcactact ttaaatcaag
    38461 ttaatgatat ttcggcaagt attaataata ttagattcaa cagagtttct gacgatatag
    38521 agctttttga aataaaaaaa ttctgcatta attctcaaaa aattctcaaa caaatatctc
    38581 catttgattt ttcattaatt ttactccacg atttaagcga agtaataaca attttagacc
    38641 ctcaaaacac tgggattcca gctttttata tttattccga ctatagtcca gaattagctc
    38701 aacttcgcaa agaaaaagac aaagcaaaaa ctcctgaaga agctgaaaat ttaagattaa
    38761 aatgtgtaga aattgaagat aagattagag aaaatttaag taaacaatta gcaaattacg
    38821 taaacaagtt agaatacaac ttaaatcgcc ttgcagattt tgacatttta attgcgaaag
    38881 caaaatttgc tgtaaaattc agattaacag agccaaaaat tacaaaagaa aagactattt
    38941 acaaaggttt atttaatcct caaataaaaa ctattttaga aagccaaaat aagaattttc
    39001 aagctataga tatagagatt ttcccatttc caactttaat tacaggtgct aatatgtcgg
    39061 gaaaatctgt tttgctaaaa accgttgctc tctcacaatt tatgtttcaa tttggatttt
    39121 ttgtgcctac tcaaaatgcc gaaattattc ttgttgaaga aattttatta aacattggcg
    39181 acaatcaaga tgagctaaac ggtctatcgt cttttgctgt agaaattcta aatataaaca
    39241 agattatttc tcatgcaaaa tcaggtaaaa aaatcttagc acttattgac gaacctgctc
    39301 gcacaacaaa tcccgacgaa ggtaaagcta ttgcaaatgc aattgtagaa atactcactg
    39361 aattgaaagt aaattcctta atttgcacac attatagtgg aattaaagct aacaaaaaac
    39421 ttagagtaaa aggattgcaa aatatttcag ataatgaaaa tataaatatc gaaaacataa
    39481 acgattttat ggattactct ctaattcagg ttgatgaaga aaaagctcca gccgaagcca
    39541 ttagaattgc cgaaattctt ggtgccgaca aagactttat tgaaaaagca aaaaaagaag
    39601 taaacaatat ttaacgtttt tgtaataagt ttgtaattct tattgttttt gtttgataaa
    39661 taaaaataaa ataagcaata tttataaaaa aatatattaa ctttacactc aatattataa
    39721 atttttaaac taactaaaaa tattttttat gaaacaattt tttaaataca cacttgctag
    39781 tttacttggc agtattttaa cctttgcaat tttaattgga attttaattg caattatttc
    39841 agcttttgta gctggcttta gcgaacaaga agcaataatc gatgacaatt ctgttttaaa
    39901 gttaagactt gcaacagata ttcccgacaa agcttccgac aatccattta gtaatattga
    39961 ttttatatca atgacttcga ctaaaactat gggattgaat aatattttaa aaaatattga
    40021 aaaagctaaa aaagacgata aaattaaggg aatatttttg gacgtaggaa gcattccttc
    40081 tggtttggga actgtagaag aaatacgtaa taaacttatt gattttaaag aatctgggaa
    40141 atttataatc gcgtattcat cgggttatac acaaaaatca tattatcttg ctagcgttgc
    40201 agataaaata tatttgaatc ctgaaggttc ttttgattgg aaagggcttt attctcaagt
    40261 aatatttttt aaaggagctt tagataaatt aggcatagaa gcacaaattt tccgtcatgg
    40321 acaattcaaa tcagctgttg aaccattttt tactgacaaa atgagcgaag ctagtaagtt
    40381 gcaaacaata actttaattc aaagtatttg ggacgacatg tgtgcaaaaa tttctaaatc
    40441 aagaaatata gaaatcgaac agttaaatct tattgctgat agcttatggg cttatgatgc
    40501 caaaactgca aaagaataca agtttgttga cggtttagtt tattatgacg aagttttagc
    40561 tgaattaaaa acaaagtcag cgtctgaaga agccccagaa gattttattg tgagcttagg
    40621 tgattatgca agtgtaaaaa ttaagacaaa ggcaaataat tctaaggaca aaatagcagt
    40681 aatttttgct gaaggcgaaa ttattgacgg tggaaaaagc gaaggttcac ttgctggcga
    40741 ctggatgtct gaaattatac gagaagtaag agaagacgaa gacattaaag ctgtagtatt
    40801 aagagtaaat tctcctggag gctctggatt ggcttctgat attatgtggc gtgaaataac
    40861 tttaacaaaa gaaaagaaac ctgtagttgt atcaatggga aatcttgcag cttcgggagg
    40921 ttattatatc gcttgtcctg caaactatat tttcgcttta ccaagtacat taactggttc
    40981 tattggagtt tttggtatgc ttccaaatgt tgaaaaacta atgacagaaa aacttggtat
    41041 aactgttgat ggcgttggca caaacgcaca atctgacttt ggacaaataa ctagaaaaac
    41101 cacaactagc gaagctaatc taatacaaaa tcaagttgaa gaattttatg atacatttat
    41161 acaaaaagtt gctgacggaa gaggtttaac aaaagctgaa gttgattcaa ttggtcaagg
    41221 tagagtgtgg agtggactta atgctataga aattggctta gttgatgaaa ttggcggatt
    41281 aaacgatgct gtagccaaag ctgcaagtct agctaatcta agcgagtaca gacttataga
    41341 atatccacaa aaagaagatg cttttgaaac atttataaaa gactataaca aaaatgttac
    41401 tgaaaaagta attaaagaaa cactaaataa aaactacaaa ttttggcaaa taattaaaaa
    41461 tgctcaaaat atgtctggaa ttcaagctag aatggaatat gaactagaaa taaattaaaa
    41521 aaacttgaat ttctaagtct aactttttaa taagttaatt tgcttctttt ataagatatt
    41581 ttgtgagatt gttgaattta cgcttcatca gttttgccat aaaaatattg aatccaatct
    41641 tgatatctat cttgtgccga aagacaagta tctattaaat aagctcttac agaattaaat
    41701 tctatacaac ggtttcgaat atataacgag tctggtcttc tgtcaaacga tttccctcga
    41761 tgcgattaga attgtaagca aaatttattt gggtattatg gtataagcaa ccgtttattc
    41821 ttaaatcttt ttcgtttata agttgcgttt ttaaatccat tttaaaatta ttttccttac
    41881 taaattatgt aattagaaaa atttattaaa atttacgcat atttctcaaa aatagaaagc
    41941 atcgtcataa ggttaaagct ctttttttgt ttttttttca aaatcttatt tataaaaaac
    42001 gctaaactca atacaaacaa tggagctata caaacagaaa taatcaaaaa taaatttcca
    42061 ttttctagat ggttaatttt taaataagcc ccataaattg aaaaaaatat tgtcaataaa
    42121 cctaaaactg ttgtcaataa gcctaaaatt aatagcaggt atttatttcc cataattata
    42181 tgttttatcc tttctatgca aatataaaca ttttttttta cattatctca accttaaacc
    42241 gtctaaatct tgcagatttc cattaaacac attcatgtca acatcacctt taattccttt
    42301 aactcttgct ctgtcggtaa attgccaaaa cacccaatct ttgttaattt tatttggatt
    42361 ttttttggta ttataccacg caatccacaa aggatattta tcaaaacgct tatcatgaac
    42421 aaaggtgttt aaataaaaat tatggtaagt atataaaata ggtgttactc cataatgttc
    42481 ttctactatt tgaagccagt tgagcaaacc gtcttttaat tttggtaaat tttgaattcg
    42541 gctatatttt tcaatatcta aaattggtgg taaatctcct ttttcaagtt ttacggtttt
    42601 tataaaaaaa tttgcttgct ctgtactatt ttcattagga cgataataat gatatgcacc
    42661 tcttaataaa ttttgttttt cagcctgttt ccaattataa tcaaatttag tgtccttgtg
    42721 attttttcct gcacaagcac gaataaatac aaaatcaggt ttttcttcag tctttaaggc
    42781 tttccaatca attttacctt gatattgcga aacgtctatc ccataaacag aatagccttc
    42841 aagctttaat tttgaaaaat taccctttac atgttgattt ggaaagaaaa agctttgaat
    42901 ttcttcgtaa taccaataag tcaggaaagc aattattgct aaagcaataa ctaaaaatat
    42961 attcctccta tttttatttt ttttctttct tgaccttctt cttttttttg ccattattca
    43021 ataaaattgt cacaaatata ttgtgctatt tgaactgcat tgcttgctgc tccttttcgt
    43081 aaattatctg ctactatcca catattcact gcattttcac atgattcatc tcttcttacc
    43141 cttcccacaa aaataagatt tgaattttca gcagttaatg gcattggata aatacttttt
    43201 tgaggattat ctaaaagact aacaccgtct gtttcttcta aaatatctct aagctcagaa
    43261 atgtcaaact cattttcaaa ctcaacattt acagattcgc tatgtccacc taaaactggc
    43321 acacgcacaa ctgtagaagt aagtttgagt tcgggcaaat tcaaaatttt tcgagtttca
    43381 tgaactaatt tcatttcttc ttcggtataa tcattttcta aaaattcgcc accttgtgga
    43441 atacaattca aatcaatttg atgtggataa gccatatttt caggcttttt ccccgctctt
    43501 tctaccatta attggttaac tcctttatgt cctgttccgg taacagattg ataagtagaa
    43561 acaactactc gttttagttt atacttcaaa tgtaaagccc acaatactac tgcaagctga
    43621 atagtactac aatttgggtt tgcaattatc ttattataat tttctaaaat agaaatattt
    43681 acttctggaa ttattaatgg aatgtcttta tccattcgcc aagcagaaga attatcaaca
    43741 acaaaacaat tattacttgc aaattttgga gcccaatcta aagaaacgga ttttcctgcc
    43801 gaaaaaatcg caatgtcagg tttttgctct aaagcttgtt caagacttac aactttatat
    43861 tcttttccta ataaatatac tgacttccct acagattttt ctgatgcaac tggaataaat
    43921 tcatcaaatt caaatttata ttcttctaaa actttaagta aacttgcacc taccattccg
    43981 gtaactccaa ctactgctaa tttcataagc caaaaatttt ttctattatg taaaaataca
    44041 agattttttt aattttatct gattttacta agtttatttt aaaatttcaa atttaatttt
    44101 tctaaaatca tatttatgag aaataatatt tttaaaagag caaaaaatta ttaactttgt
    44161 ttgctaaaat tacacatatt tttaaaataa aaaattatta tatctatgta ttcaaattat
    44221 caaaaatttt tacaagaaga aatttcagag attaaaaaag cagggcttta caaatctgaa
    44281 agagttattg tttctccaca aggagcagaa atagaagtta gtagtggtca aaaagtttta
    44341 aacttttgtg ctaataatta tttaggttta gcaaataatc ctaagcttat agaagctgca
    44401 aaaaatgctt tagacactca tggttatgga atgtcgtcgg ttagatttat ttgcggaact
    44461 ggagatttgc acaaagaatt agaaagaaaa attgcaaact ttttcggaac tgaagacact
    44521 attctctatg ctgcttgttt tgatgctaat ggtggagttt tcgagccttt atttactcaa
    44581 gaagacggaa ttattagtga cacattaaat catgcttcaa tcattgacgg agttagatta
    44641 tctaaggcac aacgttttag atttaaaaat gccgacatgg aagatttaga aaaacaatta
    44701 attgctgcta aagattgcag atttaaaatt attgttactg acggtgtttt ctctatggac
    44761 ggaaatgttg ctcctcttga taaaatttat gaactagcaa acaaatatga tgcgttgatt
    44821 atggttgacg aatctcactc tgctggagta gttggaaaaa ctggtcgtgg cattactgaa
    44881 cattttaatt tgatgggaaa agttgaaatt atcaccggaa ccttaggtaa agcatttggt
    44941 ggtgctattg gtggttttac aactggcaaa aaagaaatta tagaaatgtt acgtcaaaga
    45001 tcaagaccat atttattctc aaactcaatt ccgccacttg ttgctgcagc aggaataaaa
    45061 atgattgaaa tgatgagcga aacaaatgag ctacaagata aacttcatga taataccgag
    45121 tattttatga aaaaaatgag caaacttgga ttcgacatta aaccaacaga atcagctatt
    45181 tgtgctatca tgttgtatga tgctaaactt gctcaagatt ttgctgcaaa acttttagac
    45241 gaaggtattt atgtaattgg cttttctttc ccagtagtac ctcgcaacga agctagaatt
    45301 agagtacaac tttcagcagc ccacgaaaaa gaacatttag ataaagctat tgctgctttt
    45361 gaaaaaatcg gaaaggaaat gggagtttta aaataaaaac attctgttaa aagtcaaaaa
    45421 gtaggcggtg tttcataagt gtcaaactgc tgacctgagt ttataaagtt aaaagttcat
    45481 aaagttcctt aaatccgcaa ctaattatct agaattaaaa acaaagcctt gcaaataaaa
    45541 tgttgcaagg ctttgcagtt tcaaaaaaaa tcgttttgcg gctttgcgaa ggtggcgatt
    45601 ttcaccacta aacttcattc gaagcactaa attttgataa accacttcac tgtcatacga
    45661 agcactg
//
LOCUS       DAOPNX010000009        44698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_1089_length_44698_cov_6.040857, whole genome shotgun sequence.
ACCESSION   DAOPNX010000009 DAOPNX010000000
VERSION     DAOPNX010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 44698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44698
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1089_length_44698_cov_6.040857"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(317..1540)
                     /locus_tag="PKN53_01855"
     CDS             complement(317..1540)
                     /locus_tag="PKN53_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87730.1"
                     /translation="MTHLKYFFAIIFSLFLENAYCCTTAVISGKATKDGRPIIWKLRD
                     TDFLENYAKKFPAEKGKYAFIGIVNSIDSLGKEVWGGNNELGFAIMNSASFNVNLNDS
                     AKVKDNEGYFMKKALETCKNLEDFENLLNNTPKPMGLATHFGVIDAKGGAAFYEVNNY
                     TWTKYDANDSNIAPEGFILRTNFSETGKPNIGYGFVRLQTAKLIFDKAIKENSIDYRD
                     IIQKFSRCLYNPITKNDYREIYEKETASDKFIHSDNLITSYGSASCIVIQGVKNNEVL
                     QFTTMWTMIGYPNTCIALPLWASESELPNAVIYNDSLKNSKLNHYNMKLFKECYPIDN
                     PDGYHYLQISKLVNKENNGYIQIIEPIERNIFDETEKKLAKWRNKTPPKSEIENFYTE
                     LNDIVEKTYEKLIEK"
     gene            1683..2096
                     /locus_tag="PKN53_01860"
     CDS             1683..2096
                     /locus_tag="PKN53_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87731.1"
                     /translation="MKVKIIKIILPTILGLLTVLGVLVVLNFIIYDGDAFSKPDNGFF
                     TIFVPISIFIAMIIQLVSLPFWEKFKSYKKVWGLTLFQFTTILCIISGLIFGLVFWER
                     SFGFGEFIAVSITGIIAFAIYWTVNLITIKQIEKL"
     gene            2116..2772
                     /locus_tag="PKN53_01865"
     CDS             2116..2772
                     /locus_tag="PKN53_01865"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HOD87732.1"
                     /translation="MPKFYFLFCLSFFILFLSCENKNDEFNLQTGDLLFSVGKEESGL
                     HNAIQSSTGQNTELAFTHVGIVNVENEKTHVLEAIPSGGVVKTPLEDYFENSAFVDNK
                     YLIAVGRVKKEFEYIIPNAIKNAEKNLGKKYDFAYDESNDESYCSELVRFAYLDSLQN
                     PIFESLSMTFKNSKTNSIDAYWVQHFEKLGKPVPEGEPGTNPADMSKSPVIEIVYRYY
                     "
     gene            2899..4374
                     /locus_tag="PKN53_01870"
     CDS             2899..4374
                     /locus_tag="PKN53_01870"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOD87733.1"
                     /translation="MNKQLKKLAGQTVIYGFSSVLGRLLNYLLVPLYTRLFLPEVYGI
                     VTELYAYVTFLLILLTYGMETGLFRFGNDKQNFNKVYSSILFSLTVTSSVFIIIVLVF
                     LEPIASAMQYSEHPEYILWMALIVALDAFTSIPFARLRLENKAWKFAIIKLINICTNI
                     GFNLFFLIYCPKLLNSNPDSFILNIYSPNIGVGYVFISNLLANIITLIILLPEIFNVK
                     IKVDFKLLKTILKYSLPLLVAGFAGMINETLDRVMLKHLVSEELNPMAQLGIYGANYK
                     LAVLMTLFIQMFRYAAEPFFFQNKNESNARQLYAKATKYFIIFGLMIFLGVMFYIDII
                     KYFISPNYHEGLKVVPILLIANLFLGIFFNFSIWYKLNDMTKYGAWLAIFGAVITIVL
                     NLVLVPIYGYVGAAWATLVCYFLMTLLSFYWGQKYYKVPYEVGNGVFYFVLALSLYFV
                     SVFLKPESQTLMYVLNTILLLVFVAIFVVKEKFWREFVKVK"
     gene            complement(4491..4871)
                     /locus_tag="PKN53_01875"
     CDS             complement(4491..4871)
                     /locus_tag="PKN53_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87734.1"
                     /translation="MKNYICPKCNGYLSIDNEIVFLTKNNKGESAIVLLSAELGNYSF
                     RKNDNVDFEEGDHVNFICPICYENLNAENIDDNLACVKMIDSNGKAKKIMFSKVLGEK
                     CTYAISDEGVDSFGDNKDTYSNRF"
     gene            complement(4960..5142)
                     /locus_tag="PKN53_01880"
     CDS             complement(4960..5142)
                     /locus_tag="PKN53_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87735.1"
                     /translation="MKEIENKEEWNFILKETNKKDRNNHIKREILFALQIELSKPKPN
                     MDFYKEMKMKYLSLDD"
     gene            complement(5153..5833)
                     /locus_tag="PKN53_01885"
     CDS             complement(5153..5833)
                     /locus_tag="PKN53_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOD87736.1"
                     /translation="MKTNDLILESTTLWDFPTQNYGEMPHGNNKYNGVTPAFVIWNLL
                     QRYTKENDLVVDPMCGSGTTIDVAKELNRKVIGYDINVVRPDVIKNDARQIPLKNETV
                     DFIFIDSPYSNNINYSDNPLCFGKISCEKTEFYDELEKVMIEAKRVLKKNKVIAWLIG
                     DQWIKKKFTPTGFLLYQRLEKHFEPIDLICVTRHNQTSNTGLWHNRARRYNFYLRGFK
                     YLIIMRKI"
     gene            complement(5830..6504)
                     /locus_tag="PKN53_01890"
     CDS             complement(5830..6504)
                     /locus_tag="PKN53_01890"
                     /inference="COORDINATES: protein motif:HMM:NF036920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsaWI family type II restriction enzyme"
                     /protein_id="HOD87737.1"
                     /translation="MTFKELQELYDKKKATFGKDAYKHISEIFEEAKALHKKDFKGRD
                     HEQSWRAFKGKNLEKLIEYIISDEIKRIGLDVVNGNSLERTECANLSKKLGKVKRNLA
                     IDFGSEYGGLHMPDVDIVIFNPKDCEIIAVLSVKVTLRERIAQTGYWKLKLAADKNTK
                     HIKVFFVTPDEDGTLTKKKPFKKGRAIVEKDLDGGYVMTEAKIETSDKIKMFDKLVAD
                     LKKIIK"
     gene            complement(6501..7784)
                     /locus_tag="PKN53_01895"
     CDS             complement(6501..7784)
                     /locus_tag="PKN53_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOD87738.1"
                     /translation="MTEYFDNIEIDYSYSFTDKTTKDTSYITHGYYTYPAKFIPQLAE
                     RLIKENSKEGDIVIDPFMGSGTTVVESIVNNRIGIGTDINEIAFLLSKVKTTPIKNSD
                     LLTEYAKFEQDLPYKLDGSFDYFLNKAQRYIPENERIDYWFLPEQKDKLSVIFAQILE
                     IQNKNVQDFYLIAFAQILKSCSIWLQKSIKPTRDKKKKVYDPLALFLQQSKKMIKKHY
                     EFDKLLNKNVKENIDLYRNVSCGDSRNLNCETEKANLIVTSPPYVTSYEYADLHQLPS
                     LWFGYLNDLPEFRQKFIGSAYKNRENIDLKSNLANEIVSKLKNKKQIEVENYFADMLE
                     TFKEMKRVLKRGGRACVVIGNTEFKGVQIKNAEVFQEQFHNIGFKTHDVIKREIPSKM
                     LPSTRNPKTGRFGKISDKNIKLAYPTEYILITEKI"
     gene            8027..10117
                     /locus_tag="PKN53_01900"
     CDS             8027..10117
                     /locus_tag="PKN53_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOD87739.1"
                     /translation="MKCKILSLFLCFPLLMLAQQKPSQQWLDQKFSMFIHFGLYSVYG
                     GVYDGKPVERGYSEQIQSFAGIFSDWYGNTAKQFNPTKWNPDEIVKLAKDAGMRSIVF
                     TSKHHDGFCMYHSQYTDFNIVDATPYGRDLMKELADACKRGGVDFAVYYSLIDWNYPH
                     AYPISSHNADPLTDEHYEFNKKQVEEIMTNYGDISEIWFDMGSLTPAQSKGLYDLVKR
                     LQPQCMISGRLGNDYADFSVMPDNKYPDYKLGIPWQTAASMFDETWGYRSWQKRGSVQ
                     EKVDEKITSLIKVISRGGNYLLNIGPRGDGSVVEFERDVLLEMGKWIKANSEAIYGTS
                     ANPFDHTFNWGEVTTKNGDLYLFVKKDFYGKDIGISGFKGSIAEVKVLETNELCDFYP
                     PKKYGDDWNIRLPKTTSELPYSVLKVSFENSFAILPKIVANTVLTPQNSSPVFGHSSL
                     NYYCGYKSLIGYDWAFNTTKRHINPEIMFSENELGREIIVEIDENKQNITLNSSNKKI
                     VRLVQNTVKWGDLYRKRSSMVFGNLTDLEGNPNIIPDDKWTKINQFNYGQIVTEEVLS
                     KSGTVFLQEIESIKEQKIAIEIKSGNALYILLNGEYITAHFSPDRVNSNQEIVFLPLK
                     KGKNQLAIVFYNGFANALSYKIEALQQWTVYTQKLSSSKLNKEKQFHTLSVRDANPIS
                     NVGPLRMNNIEIKF"
     gene            10121..10258
                     /locus_tag="PKN53_01905"
     CDS             10121..10258
                     /locus_tag="PKN53_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87740.1"
                     /translation="MKSKLNNKNTTLGKSASVVSVILKGLGQIMLQTYIYKFIVSYSL
                     F"
     gene            complement(10353..11156)
                     /gene="rhuM"
                     /locus_tag="PKN53_01910"
                     /pseudo
     CDS             complement(10353..11156)
                     /gene="rhuM"
                     /locus_tag="PKN53_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795764.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            complement(11153..11740)
                     /locus_tag="PKN53_01915"
     CDS             complement(11153..11740)
                     /locus_tag="PKN53_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOD87741.1"
                     /translation="MTNFRIHTRRSIRLKGYDYSQEGLYFITICVQDKICRFGHIENG
                     KMIYNQYGTIAHNEWINTPNIRTNIILHEFVIMPNHIHGIIEITEKLSYNGAYDTPSE
                     SEIGEYDTPQRTDELHSSVRSHLRSPSQTIGAIVRGYKSAVTKQLNALGLTEKLWQRN
                     YYEHIIRDEQSYIRITEYIRNNPKKWADDKIKIKK"
     gene            complement(11824..15123)
                     /locus_tag="PKN53_01920"
     CDS             complement(11824..15123)
                     /locus_tag="PKN53_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HOD87742.1"
                     /translation="MKLKIYILILLSLSFISLKAQPDSTDCFISYNSIWAKSLGSNMF
                     DEVLGVASDNSGNIVVVGQFHGSLNFQSQTVVSNGGSDFFVAKLDSFGNLIWLQSGGG
                     TENDVAKAVSIDSQGKINVIGNFRGTLNIGGNQINSNGESDIFIIQYSPDGNYLWGKF
                     LSGFYEDSGNDICVDADDNILVTGYYDQFLGIGNGTLIAKGAKDFLLAKYDANGNFLW
                     RANHGSTQNDIATSIACDKDNNIFIAGEFSGTINFGSLNLSANGSLNTFVAKYNKSGV
                     FQWAKAFGSNSNTASQASVATDIFGNAYISYKSELNNNMAKIHKFNPEGIEILNKSFG
                     GLGNIIPNDIKVDYGQNIYIAGKFNGLTDFGDGNINSTANFDCFLSKLDNNGVFKKKE
                     LKGVSDTNSFNAMCINELNQLIVAGSFSNTLSFNSDILNSNGNEDGLIVKYGLGFGFS
                     EIIISSNNCDPNDMCIDIQTINGVPPFTYYCNNTLISQNICGLSVGNYEIIVVDKNNC
                     YIKTKIELQALQGPQIAIPTNITLCPYESIVLNAGNGDYEYLWSTNETSQIIEINEGG
                     VYSVTVTDLGTNCSTSASTNVTKIPNPALLPETDSLCFGDELTITTFDTYASYFWSDA
                     SINQYFTSGNSGIHWVKVFDSNTHCHYYDTITFVYFPTIDLNLEKNIVICDGDSVLVS
                     APPGLASYHWSNNTFSESIWLSQAGTYSLTVTDQYSCTASSEINVGLGESPIINLGED
                     FSICTNEAVVLSANAQGDNLTYLWNDNSTEETLTITSSGTYWVKVSALTGCDAIDTIK
                     VDIFPQPVLDLGNDIEFCYGETYLIEVDSTYATYQWSNNLTTSTISVNSTQTLFLTVT
                     DSNNCTATGKINVIEHDLIEPFIGYDTTFCEGNTYILKTDREYFKYKWQNGSNASSFT
                     VTQAGNYALTVYDEIGCSGSTEINVEFLEGPVWDSYTSGGGMIEVFASGGVQPYVYSY
                     DGDTWQTSNIFTNLPSGVYTISIMDDNYCLISKEIFLDESLSIPNFFTPNGDGYNDTW
                     VITGLYHYPDAVVQIFDRFGKKLYSSTGADFSWDGTYAGKELPSETYWYVITLDSNKK
                     QVLKGSITIKR"
     gene            complement(15127..16143)
                     /locus_tag="PKN53_01925"
     CDS             complement(15127..16143)
                     /locus_tag="PKN53_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOD87743.1"
                     /translation="MLKIKNILLVIFATTLFCTELKSQDLPVHEQYMFDFMLVNPSFT
                     GLTQSSIFKAIHRQQWIGFENAPKTSLFLFKHRLKGREGGIGGYVFSDLNGPNQRFGA
                     QFNFSYQVFLYNKRNHKFIMSFGMAFRGWAHVLDQTKFTRNMYDPIVTYSKETTVNPN
                     ASAGVLFSYNHTFLGVAFDNLLPWTDRMYNRSLEPINHVLTNLHAGSILQIHKKVQLR
                     PSCLFKSNFHGLNQLDVNLKVHYGGTSSRKSKFVRKDNDMWFGLSYRNTLDKGNSSPL
                     SIAPSFGMSIDQFSIFYQYEIGLTSLQLYHNGTHQIGLGLKIFHDKYIKWGKFHNAIF
                     YDEF"
     gene            complement(16158..16829)
                     /locus_tag="PKN53_01930"
     CDS             complement(16158..16829)
                     /locus_tag="PKN53_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOD87744.1"
                     /translation="MSEIKKNIDNILKQIPNNTKLVAVSKTHSIETIKETFDAGQRDF
                     GENKVQELVDKHNQLQNPEIKWHLIGHLQRNKVKYIADFVELIHSVDSLKLLTEINRQ
                     AKKHDRVINCLLQFHIADEDTKFGLNYDEAIDILESDEFKSFENIKICGVMGMATFTD
                     DTEQVRKEFKKLKEIFSEIKDKYFKNIDYFCEISMGMSGDWKIAIEEGATILRIGSII
                     FGDRT"
     gene            complement(16835..17533)
                     /locus_tag="PKN53_01935"
     CDS             complement(16835..17533)
                     /locus_tag="PKN53_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87745.1"
                     /translation="MKKQDLIFILAIIIFFLPFFVFDEVYNFYNDFNNKYSLIMAFIK
                     FAILATLGESIGLRIKTGVYNQKGFGLLPRAIAWGFFGMWIAIAMGVFRHGIPEFLDK
                     FSIFNGIAFAMNGDFTALKLVGAFFISLMMNTTFAPVFMTLHKITDTHIMNNGGKVSA
                     FLKPIPIKKIITNLNWEVQWGFVFKKTIPLFWIPAHTITFILPQNYQVLTAALLGVVL
                     GVLLSIAAIQGNKK"
     gene            complement(17636..19693)
                     /locus_tag="PKN53_01940"
     CDS             complement(17636..19693)
                     /locus_tag="PKN53_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOD87746.1"
                     /translation="MINDKLLNPKSVVVVGGSNDVYKPGGKVLKNLIDNDFKGNLYVV
                     NPKLDEVQGIKAFRDVNDLPQVDCAIIAIAAKFCYDTVETLVKQKATGGFIILSAGFS
                     EENEEGAKLEKKIVDLLNEHKASLIGPNCVGLLNQTYSGIFTTPIPKLDSQGIDFISG
                     SGATAVFIIESAVSKGLKFSSVYSVGNSAQLGVEEILKYLDETYVHGKSAPVKLLYVE
                     SINKPKMLYKHAASLIRKGAKIATVKSGTSEAGSRAASSHTGALASPDVAVEALFRKA
                     GIVRCNGREELATIASVFMHPTPKGKNVAIITHAGGPAVMLTDALSNGGLNVPAIEGP
                     KAQELLDKLFGGSSVANPIDFLATGTAEQLGFIIDACNNDFDNIDSMAVIFGSPGLFP
                     VYDVYDVLHEKMKTSKKPIYPILPSIINVKDEIEKFIEKGRINFPDEIAFGNALSKIL
                     NTPKPQAENIELPKIDKERIRKVIDSAGNGYLSPDKIYELLDAAGIDRVIEKVVTTKE
                     DALAFAEKHGFPVVMKVVGPVHKSDVGGVVLNVKDNETIVREFDRLMKIKDTYAVLMG
                     EMISGTEVFIGAKREDKFGHMVLAGLGGIFIEVLHDVNASLSPVSKNEAEYMIKNLKS
                     YKIIEGVRGQEGINQEIFIDQIQRISALVETAPEIFEMDLNPLLGNMKYVKAVDARIR
                     IEK"
     gene            19937..20926
                     /locus_tag="PKN53_01945"
     CDS             19937..20926
                     /locus_tag="PKN53_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOD87747.1"
                     /translation="MEKKVFLISRTDSIGDVILTLPICAWIKNKFQNSYIIFLGNSYT
                     KPIVECYPYVDEIIDWRELKGLDKKTQINILKQKNIDVCIHVFPKKEIAKLAKKLKIP
                     TRIGTSHRVFHFFTCNLRPNFSRRKSDLHESQLNFELLRPLGLEKIPSLAEIQEYLSS
                     FIIPKVHLDFDLSAKNKIILHTKSKGSAVEWPMNKFFELAVKLADLDYEVYFTGTEAE
                     AVHFRDKLPKHQKIHDMSAKFTLTEFIYFISQCEVIVAGSTGPLHIGAVSGIKTIGIY
                     SQTRPIHQARWRPIGKNVSIVTNKQEPEIGKINPQYLKDIEVDEVFKLVIADK"
     gene            complement(20931..22331)
                     /locus_tag="PKN53_01950"
     CDS             complement(20931..22331)
                     /locus_tag="PKN53_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOD87748.1"
                     /translation="MLTHKIFKLIRDFDETDNTEVYVIGGYVRDKLLKRADKKDIDIV
                     VKGNGIEFAQKLANKLKIKKLSIYKTYGTAMFQFEDTELEFVGARKESYSPESRNPKV
                     EQGSIEDDQKRRDFTINALAISLNKETYGEITDPFNGIEDLHYRTIRTPLDPDITFSD
                     DPLRMMRAIRFASQLDFIIDDECFEAIKRNKHRIEIISAERIHTELNKILLSKQPSIG
                     LKLLFDSELLEIIFPELYNLAGVSTQAGVKHKDNFLHTIQVVDNVAENSDNLWLRWAA
                     LLHDIAKPKTKKYVEGIGWTFHAHNFIGQKMVPEIFARLKLPLNEKMKYVQKLVDLHM
                     RPIALVEDEVTDSAIRRLIVDAGEDLDDLLTLCEADITSKNEKKVKQYLTNFKALRQK
                     IVEVEERDSLRNFQPPISGDEIISYFNIEPSRTVGIIKNAIKDAILDGEIPNDKEKAW
                     EYMISKAKSLGIERQE"
     gene            22427..22786
                     /gene="trxA"
                     /locus_tag="PKN53_01955"
     CDS             22427..22786
                     /gene="trxA"
                     /locus_tag="PKN53_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOD87749.1"
                     /translation="MAEHLTKEKFIERIFDYENETEWKYKGESPVFIDFYADWCGPCK
                     MIAPVVEELSKEYEGLIDIYKVDTEDQQELASIFGIRSIPSLLFIPLSGQPQMMTGAV
                     PKDGLIQIFEDVFKIKK"
     gene            22942..24426
                     /gene="hutH"
                     /locus_tag="PKN53_01960"
     CDS             22942..24426
                     /gene="hutH"
                     /locus_tag="PKN53_01960"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOD87750.1"
                     /translation="MNTHFITTEHLSLDKLQEIFKNGYKIELSEQAKNNIIKCRTYLD
                     NKIKTHDKPIYGISTGFGSLCNTNIASEDLEQLQTNLVISHACGMGAEIDSEIVKLMM
                     LLKVQALSYGISGVCLETVERLVYFYNNDIRPVVYELGSLGASGDLAPLAHITLALIG
                     LGEVNYKGKRQKTETVLSELNLKPLKLKSKEGLALMNGTQFMLAHAITGVWRAEKIMF
                     NADRIASLSLDGYNGLIAAFYPSLHTIRPHKGQIETAKNIRTFLEGSEIVAQTKTHVQ
                     DPYSFRCIPQVHGASKDTLNYVKSVVETELNSVTDNPMVFPDEDLVLSGGNFHGQALA
                     LVLDFLSIAVSEIGNISERRIYRLISGDRGLPAFLVANPGLNSGFMIPQYTAASMVSQ
                     NKQLCTPASVDSISSSNEQEDHVSMGANAATKLHRVIANTERIIGIELFAASQALSFR
                     EPLKTSPELQKYLNKFRKIVPLVKDDIVMYEMLHKAEEFVKSDE"
     gene            24538..25944
                     /locus_tag="PKN53_01965"
     CDS             24538..25944
                     /locus_tag="PKN53_01965"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD87751.1"
                     /translation="MKIKFIGAAQEVTGSKHLLTLDNGKKILLDCGMFQGKGMETDRM
                     NRNLGFEPKEIDHIILSHAHIDHSGLIPYFYTQGFEGSVICTDATRNLCSYMLPDSGY
                     IQEQDIKWYNKKLLKQGLLPIEPIYRHSDAVKSMKLFIGVAMDRKFRIDENITVKFTD
                     TGHILGGAVVNLEIKENGKTTKIAYTGDIGRPYNKIIKPPAAFPQADYLITESTYGNR
                     LHEDKADAEEKLFNVIVDTCVRKGGKLIIPSFSVGRTQEIVLLMNNWYNEGRLPNIDV
                     YVDSPLSVNITEVFKMHSECYNDEVLEVLTYDPDPFGFEGLHYIKNVNASKKLNDTTE
                     PCVIISASGMAEAGRVKHHIANSISNPKNTILLVGYCAPTTLGARLQEPGLKKISIFG
                     TEHEVRASVKLLEAFSGHGDYVEMAEFISCQDKDLLKKIFLVHGDLESGEHYRKYLLK
                     MGFKNIEIPKPFDEVELV"
     gene            complement(25962..27740)
                     /gene="argS"
                     /locus_tag="PKN53_01970"
     CDS             complement(25962..27740)
                     /gene="argS"
                     /locus_tag="PKN53_01970"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOD87752.1"
                     /translation="MEINIKKYIKEALNSLYSVDTTNIDVAVEKTKEGINGDFTVVVF
                     AFSKFSKKSPADTAKDLGAFIKQKYDDVSSYNVINGFLNFRMTNEYWIDRLNSLNLNN
                     KKSEEFDKETIMVEFSSPNTNKPLHLGHIRNNILGDSVSRILKASGNHVIKTNLINDR
                     GIHICKSMLAWMKWGNGETPETSGKKGDKLVGDYYVKFEAEYKKQIEELIKAGQTETQ
                     AKANAEIILEAHEILRKWENGDEEIRKTWRMMNSWVYKGFEQTYSDLGISFDKVYYES
                     NTYLLGKDIVKQGLENEIFVRDEDSSVWIDLTDEGLDRKILLRSDGTTVYMTQDIGTA
                     IKRIEEFNPSKIIYVVGNEQDYHFAVLKKIISKINKSYSDVIEHLSYGMVELPEGKMK
                     SREGKVVDADDLMSDMIETARNISNEAGKIHDLEDDEKEKIIRMIALGALKYFIIKVD
                     PKKQIIFNPKESIDFNGNTGPFIQYTHARIKSILRNAEKMNIKFHGKASEEADIVDLE
                     IELISHLCTFEKLIQESANRLDPSLIANYTYALTKLFNQYYHEHSILKETESKTLQLR
                     LLLAQTVAQTIKSAMDLLGIEVPEIM"
     gene            complement(27787..28431)
                     /gene="nth"
                     /locus_tag="PKN53_01975"
     CDS             complement(27787..28431)
                     /gene="nth"
                     /locus_tag="PKN53_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOD87753.1"
                     /translation="MTIKEKYKYVLDYFRENIKLAETELDYQNDFQLLVAVILSAQCT
                     DKRVNLITPALFNDYPDAQTMSFASPEVIFEYIKSCTYPNNKAKYLAQTAKILTEKYD
                     GKVPEDVDELQKLPGVGRKTAHVIASVLFRQPKMPVDTHVFRVSARIGLTKNAKTVLQ
                     TEKQLVKHIPEKDIPDAHHWLILHGRYVCTARNPKCKTCGISIYCDFYQQNDKS"
     gene            complement(28555..29484)
                     /gene="trxB"
                     /locus_tag="PKN53_01980"
     CDS             complement(28555..29484)
                     /gene="trxB"
                     /locus_tag="PKN53_01980"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOD87754.1"
                     /translation="MSEKIKCLIIGSGPAGYTAAIYTARANLNPVLYEGLQPGGQLTT
                     TTEIDNFPGYPEGVSGPIMMDQLKAQAQRFGTDVRFGCITKVDFSKRPFKVVADNVHE
                     IEAETVIIATGATANYLGLESEEKFKGLGVSACATCDGFFYRNKDVAVVGGGDTAAEE
                     ASYLAGLCNKVYMIVRRDVLRASKAMQEKVMNNPKIEILWNHNTVEVLGDSSGVTGAR
                     LRNNKTNEEKNIDIHGFFLAIGHTPATSLFKDYIELDEQSYIKTKPGTSKTNVPGVFA
                     CGDVQDPNYRQAITAAGSGCKAAIDAERFLSEN"
     gene            complement(29507..29959)
                     /locus_tag="PKN53_01985"
     CDS             complement(29507..29959)
                     /locus_tag="PKN53_01985"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HOD87755.1"
                     /translation="MKILTLISFVFLMSLSAFSQENPVTWNINQSRSEDGSFNFHIKA
                     DIKNNWYVYGMNLDEGGPLPLKLDIKDKEDMVYNADIKEVTPAITVYDDVFTMNVSSY
                     QGNSEFVINYVPKTEINSLTIIIDGQACNTENGMCVLVYEEIPITIQK"
     gene            complement(30117..31049)
                     /locus_tag="PKN53_01990"
     CDS             complement(30117..31049)
                     /locus_tag="PKN53_01990"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HOD87756.1"
                     /translation="MKMKLRNIIFLLLIIITSSCIKDISLELPKADEKLVIEAMIDSD
                     DYPIVFISKNSSYFTNIDTTILNASIVKGSDACVIVSNGAITDTLKPTIMERYPYHGY
                     RGTKFKGEVNKSYDLKILYQDKEYNSTTTIPEPVPIDSVWFNLLPGNDTMGFLGFKFT
                     DPEKFGNYYTFYVKVLEQQKWYYRPSFGSHILDDKLLNGQEFSYTPLTKGYERNAYYH
                     NVWLDEDLDFLSLVCFKVGDSVSLKMSAIDANSFNFWSSVYRNVLTGSNPFTNPASVK
                     SNIYGSPANGHWIGSGTHFTNVYITDSATLILIP"
     gene            complement(31053..33431)
                     /locus_tag="PKN53_01995"
     CDS             complement(31053..33431)
                     /locus_tag="PKN53_01995"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOD87757.1"
                     /translation="MKLKLIYFAIFIFIGMPLFSQKYTVSGYIKDKHTGEDLLGANIM
                     VKDQNIGTTTNAYGFYSLSIPAGEYEIIYSFVGYEEVVEKIKLNSNKKLNISLETSAI
                     ISQEVIVSGEKNKNVEDSKMSVIKLPVETIKTLPAFMGEVDILKTLQLLPGIQSAGDG
                     NSGFYVRGGGPDQNLVLLDEATVYNAAHLFGFFSVFNADAIKDMEIYKGGMPAEYGGR
                     ISSVLDISMKNGNMNKYQVEGGIGTIASRLTVQGPIVKEKSSFIISGRRTYADILIQP
                     FIKETSPFKGSGYFFYDLNAKLNYRFSDKDRLYLSAYYGKDIFDFRSKEANFNVKIPW
                     GNGTGSIRWNHLFNSKFFSNTTLVASDYKFQTTIEMGDEDKPNEGMRFIQNSGVRDYY
                     IKQDFTLLPNPKHTIKFGLNYIYHIFTPNSVNAKLGETEYDFSSVQKQKAHDAAIYIG
                     DDFKISDKVTIYGGFRAAMFNQVGKFVRYVKDENLMNNIDTITYGKGETVAFYDSYEP
                     RASIKVSLDKNSSVKASFMQNKQFIHLASLSASTLPTDLWVPCSDVVKPQGGRQYAIG
                     YFRNFFNDHFETSVEAYYKSLYDLIEYADGALPGNDVNDNPDNYFIFGDGYSYGAELF
                     IKKRTGRFTGWIGYTWSKTERIFDDINGGEPFPAKYDRRHDLSLTTTYQITEKWTASL
                     VFVFATGNTTTLPIARYMIDGDLISEYGPRNSYRMDPYHRLDLSVTYQHKKTDKWESS
                     WNFSIFNVYNRKNPYFIYLEDKGSVETGDFTTTAKQISLIPILPSITWNFKF"
     gene            33622..35142
                     /gene="gpmI"
                     /locus_tag="PKN53_02000"
     CDS             33622..35142
                     /gene="gpmI"
                     /locus_tag="PKN53_02000"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOD87758.1"
                     /translation="MTKQKVVLMILDGWGKGNGQKADVISNAKTPNYDKFIKNFPNSE
                     LLTDGENVGLPKGQMGNSEVGHLNIGAGRVVYQDLVKINKEIESGKFFQNPVLLKAFK
                     YAKENDKQVHLLGLVSNGGVHSTEDHLLAIVKLSDDLGLKKTFIHALTDGRDTDPKSG
                     IGFLKKVTDALENSNVKLASLVGRYYTMDRDKRWERVKKGYDLMLEGIGKKSTDILKS
                     MQESYDNDITDEFIEPIVMTDKDGNPVTKIQSGDVVICFNFRTDRLRQITTVLTQKDM
                     PEHGMKTLDLEYFTMTRYDESFKGINVIYEKDNLNKTLGEVIAKNGLSQLRIAETEKY
                     AHVSFFFSGGREEKFENETRILIPSQKVATYDLKPEMSAPEVKKALMAEIEKNIHNLI
                     VVNFANCDMVGHTGVYDAIVKAVETVDTAMGEVVELAIKNDYQILIIADHGNADNALN
                     DDGTPNTAHSLNPVPCILINSEYKKIENGVLADVAPTILKMMNLAIPSEMTGKILV"
     gene            35205..35708
                     /locus_tag="PKN53_02005"
     CDS             35205..35708
                     /locus_tag="PKN53_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOD87759.1"
                     /translation="MLKILGFCGKIIITVADWFYKPFSSYIPQVTFRYGFTGGLNTVL
                     DIFLYFVFYNYVFQKQNLDLYFVTLSPHIAAFAFVFPITFTTGFLLAKYVTFTQSQLR
                     GRKQLFRYGLTVIGAIFLNYIFLKFFVEYCNIWATLSKILTTCLVIVYSYFMQRYFSF
                     KTTPVKE"
     gene            complement(35717..37519)
                     /locus_tag="PKN53_02010"
     CDS             complement(35717..37519)
                     /locus_tag="PKN53_02010"
                     /inference="COORDINATES: protein motif:HMM:NF039639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HOD87760.1"
                     /translation="MKPNSLFSKIKNQINNGNLLYALIICLLLFSIFPFIYLIQYNLP
                     GASDDMLNSHVFKEFSYFDAISEWYKTGYNGRYANSALLLLPGRPYMNVWFARTIPHL
                     SFLCLLLSAYFLVRSIFKNINIKNSILITLTFVSFYICFIPSIREFYWFSGSSVYILP
                     AILYLLMLGLLISFFNKNNNPLVIIALIVCLFFTIGSHEAWVVLCFFTILGFVIFELI
                     NKRKFKLSTYIIIACTLVLTYFFIKAPGNTNRLYNESQVFDNGDFLGSAIMSTFESMS
                     LISKWFVNFGFLLIIVAILTYPRKNEQTKYAGTKTILLVVAILLSVFISVFILKFSLG
                     HLTYPMRYRGIIPGYTIALILFLCLLLNSRNVFYIKERYKNINLSYALLLLGLIFNIT
                     SSENLKDAYVDIVSHRAKIESEQVQWVENFISSSDQETIYIPSINKGTKTLYFLEIPE
                     PNTWYAWVLKVHTNKNIFIDHSLNFEKFQFSHDSTTLSLTVNPQDEFSHTHEFPIDDI
                     KKNNFTKLRIRAIINNDSNISHENKILLVIQCGDFWKAYNIEDLKAIHNNGLFSITHN
                     INTKILNSDVLKVYFWNTEMDSLNISKFKIYLEE"
     gene            complement(37509..38459)
                     /locus_tag="PKN53_02015"
     CDS             complement(37509..38459)
                     /locus_tag="PKN53_02015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD87761.1"
                     /translation="MKKNISIIASVYNEQEGLNEFYSVLKSELLKINENYNFEVIFVN
                     DGSKDNSQKIINQIIENETSLKIKSIEFSRNFGHEAAMIAGIDNSESDFIICMDADLQ
                     HPPSKIEDMLAETEKSAEIILMNRTNREDQKAMGKLSSSLFYKTVSFLSGHRFEPNAS
                     DFFLISKDVANVLRNNFRERNRFLRGYIQIIGFNITSLDYVASARIAGTSNYNFKKLF
                     KLAFTAIFAFSEKPLRISVIISMLYFLFSIILIIVSLVSYFLENKIPSGYTTIIISQS
                     IGFAILSFLIAILSIYFGKSLSEIKDRPIYIIKNSKTNEA"
     gene            38585..39103
                     /locus_tag="PKN53_02020"
     CDS             38585..39103
                     /locus_tag="PKN53_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOD87762.1"
                     /translation="MKNRLSIHNGDITKLEVDAIVNAANKTLLGGGGVDGAIHRAAGT
                     ELLNECRKLNGCETGEAKITLGYNLPAKYVIHTVGPVWRGGNNKEAELLANAYINSLK
                     IAAEKQFETIAFPSISTGVYSFPIDKATKIAVTETIKFLEQNKFPEKVIFCVFGKEAE
                     EIYKEMFLNLNL"
     gene            complement(39152..39616)
                     /locus_tag="PKN53_02025"
     CDS             complement(39152..39616)
                     /locus_tag="PKN53_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87763.1"
                     /translation="MKTKDKILKYSILGMILIFTFIFTFCGKTETDLEWETKILGYWT
                     PTGTDNKPIFDMPNYKFDEKGRGISFLSDVSKADSFAWEIKKTELKIYYDHAPKYYVG
                     YDKYNSRSLFKIKSMSDTAFFVIQYFGTGYQKEYYMKKASFVDNGDDWDDVF"
     gene            complement(39606..40436)
                     /locus_tag="PKN53_02030"
     CDS             complement(39606..40436)
                     /locus_tag="PKN53_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87764.1"
                     /translation="MTKTINLNGIKNSFNITIVKLVFFSTLSFSFACGSTEIADSKDV
                     NQSKIHQYYSISYDASEGNEYDIFAQFRFGGNKGTTLKLSEPSQISVNNIKMKEEQSS
                     FRGCFYKLAIKDSNTFNFKFTDTENKIYSNSVVVNSIEIVKIEKLYAEKNQKIYWQGK
                     SLENKEEVIAIIEDNDNNKVEVSTDIIGSNYISLNAEDMEELVAGSGQIYMIRKYYSS
                     LQEVADDGGSVDTEYISMKMPIEIISNKTKSDKDEEDDKASKDSVVDIDNEYNEDNEN
                     "
     gene            complement(40399..42306)
                     /locus_tag="PKN53_02035"
     CDS             complement(40399..42306)
                     /locus_tag="PKN53_02035"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOD87765.1"
                     /translation="MSKFREVLLKYWGYRNFREMQEEIVESIASGKDTLGLLPTGGGK
                     SITFQTYSMATPGVCLVVTPLIALMKDQVENLQKRKIKAVAIYSGMTKIEIERALDNV
                     IYGDYKFLYLSPERLQNFYFQEKLVHMKVNLIAVDEAHCISQWGYDFRPSYLEIAKIR
                     EKLPDIPVLALTATATPKVVDDIQEKLLFKEKNVLQKSFERKNLVYYVINTEDKEKEL
                     FKLITKIEGCAVIYVRNRRKTREYAMLLQKYNISADFYHAGLDAQTRAYKQSEWQKDN
                     IRIMVCTNAFGMGIDKPDVRLVIHLDLPDSPEAYFQEAGRAGRDGKKAWAFLVTNSAD
                     KAQAKRNITTAFPDLKKVIEVYNAICNYLQIPIGDGKDMDYPFDIFELAKNFKLDVLS
                     IYNSIKILEANNMLTYSEEANTVSRIMINVNRDDLYKFQVENEALDNFIKMLLRTYTG
                     IFNDYVNISEEYLARKANTKPDTIIELLKVLARYKIISYIPRINKPLMHFNAERLEDK
                     GILFDIKELNERKERTLSRMEAMLNYATSNNKCRSQHLLAYFGETDTQRCGNCDVCLR
                     RNELGLNKYEFDIILEEIKEIINFEPCLLNELVEKIKFDEYKIIKVVQWLFENKKMIY
                     DKDNKLKWNKE"
     gene            42426..43904
                     /gene="guaB"
                     /locus_tag="PKN53_02040"
     CDS             42426..43904
                     /gene="guaB"
                     /locus_tag="PKN53_02040"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOD87766.1"
                     /translation="MKENNKIILEGLTFDDVLLIPAYSEILPSSVSLVSNFSRNIVLN
                     TPLVSAAMDTVTDSKMAIAIAREGGIGVIHKNMSIVSQAKHVYQVKRAENAMILNPVF
                     IDINGTVGDAKAIMSEYNIGGLPVVDSENKLKGIVTSRDMRFQTDMSLSIKAIMTPKE
                     RLITTEQGIELEDAKAIFMKHRIEKLPVVDKDDKLVGLITYKDITKAASAPLSCKDSH
                     GRLRVAAAVGITSDTFERMEALVDAGVDAIVIDTAHGHTKSVNEVLIQAKKKFTNIDI
                     VVGNIATAEAAKLLVKNGADGVKVGIGPGSICTTRVVAGVGMPQFSAIMNVAEALKGS
                     GVPLIADGGIRYSGDIVKALAAGANSIMAGGLFAGTEEAPGDTIIFNGRKFKVYRGMG
                     SVEAMNQGSKDRYFQDMEEDIKKLVPEGISGRVPFKGNLHEIVYQLMGGLKAGMGYCG
                     AKNIQTLHENAKFVRITSSGIVENHPHDVTITREAPNYSRSE"
     gene            43939..>44698
                     /locus_tag="PKN53_02045"
     CDS             43939..>44698
                     /locus_tag="PKN53_02045"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOD87767.1"
                     /translation="MKKIFVLLSFLVFVNLAFSQSNVILEIDDEKITTDEFLHIYKKN
                     NTNTNAMTYQAMEEYLDLFVNFKLKVHEAKLLGLDTLKSFKNELSGYRSQLAQPYLTD
                     KKVEEDILAESYERMRWDVEVSHILIKCDQSASIADTVKAWNKINDIYKKLKKGESFE
                     ELVKKYSEDEGTVPTNGSLGYKTVFNLVYEFESAMYETKVGDFSQPFRSRFGYHILKV
                     TDKRPAKGKYQIAHVMTIVPEGSSRHLWKKAEDKI"
BASE COUNT        14933 a         6779 c         6737 g        16249 t
ORIGIN      
        1 taatgttttt ttgcaaagat aacaaattat ataatagaaa gcaaattagt attagactgg
       61 ggcactttga tggtaaccgc cattttagga ttaatttttt cgtttatttt gctgtggaat
      121 ccagtgttcg caggtgttag tgtagttgtt tggacagcag tagcatttgt tataataggt
      181 gcttacactg tttatttttc ctttaaattg cgaaaactca ataaaatgat gaaagaagaa
      241 tagactgttt tgttgtctgt ttatatggtt aaaagcagtt actacaacaa taagtggtaa
      301 ctgcttttta taaatcttat ttctcaatca atttttcata agttttttcc acaatatcgt
      361 ttaattcagt gtaaaaattt tctatttctg atttaggagg tgttttattt cgccatttag
      421 ctaatttttt ttctgtttca tcaaaaatgt tcctttcaat aggttcaata atctgaatat
      481 atccattgtt ttctttattt accaatttag aaatttgtaa atagtggtat ccgtctgggt
      541 tgtctattgg gtaacattct ttaaatagct tcatattgta atgatttagc tttgaatttt
      601 ttaacgaatc attgtaaatt actgcatttg gtaattctga ttcgcttgcc catagtggca
      661 aagcaataca tgtgttagga tagcctatca ttgtccacat tgtagtaaat tgtagaactt
      721 cattattctt aacaccttga ataacaatac aagaagcaga accatagctt gtaattaaat
      781 tgtcggaatg tataaattta tcgcttgcag tttctttttc gtaaatttct ctgtaatcat
      841 tttttgtaat aggattatat aaacatcttg agaatttttg tattatatcg cgataatcta
      901 tagaattttc ttttattgct ttatcaaaaa taagttttgc agtttgcaat cttacaaagc
      961 cgtatccaat atttggtttt cctgtttcag aaaaatttgt tcttaaaata aaaccttctg
     1021 gagcaatatt tgagtcgttt gcatcatatt ttgtccaagt ataattattt acttcgtaaa
     1081 aagcagcacc accttttgca tcaattactc caaaatgagt agctaatccc ataggttttg
     1141 gagtgttgtt tagtaaattt tcaaaatctt ctaagttttt gcatgtttct aaagcttttt
     1201 tcataaaata gccttcatta tctttaactt ttgcactatc atttagattt acgttaaatg
     1261 aggcactatt cataattgca aaacctaatt cattgttacc gccccaaact tctttaccta
     1321 aactgtcaat tgaattaaca attcctataa aagcatattt tcctttttcg gcaggaaatt
     1381 ttttagcata attttctaaa aaatcagtat ctctcaattt ccaaattata ggacgaccat
     1441 ctttagtggc tttgcctgat attacagcag ttgtacagca atacgcattt tctaaaaata
     1501 atgaaaagat tatagcgaaa aaatatttca gatgtgtcat aaaacaaaat tattaacaaa
     1561 cattgcgaaa atacataaaa ttagaagaca ctaggttaat ttgtgcaatt ttttattgaa
     1621 atttgaaaca aaaaaataaa atatttcaat aatttgagcg tttaaaacta aaaaatataa
     1681 atatgaaagt taaaatcatt aaaataatat tgccaacaat acttggatta ttgacagtgc
     1741 ttggagtgtt agtagttctc aattttatca tttacgatgg agatgccttt agcaaacctg
     1801 ataatggctt ttttacaata tttgtaccta taagcatatt tattgcaatg ataattcagt
     1861 tagtatcact acctttttgg gaaaagttta agtcttataa aaaagtttgg ggattaacac
     1921 tttttcaatt tactacaata ctttgcatta tatcaggatt aatttttgga ttagtatttt
     1981 gggaaagaag ctttggcttt ggtgaattta ttgcagtttc tataactggt attattgctt
     2041 ttgccatata ctggacagta aacttaataa caataaaaca aattgaaaaa ttgtagtata
     2101 ataatatttt taattatgcc taaattctat tttttatttt gtcttagttt ttttatttta
     2161 tttctttcat gtgaaaataa aaatgatgag tttaatcttc aaactggtga tttgttattt
     2221 tctgtaggga aggaggagtc tggtttacat aatgctattc aaagctcgac aggacaaaac
     2281 acagaacttg cttttactca tgttggaatt gtaaatgttg aaaatgaaaa aactcatgta
     2341 ttggaagcaa ttccttctgg tggagttgtg aaaacacctt tggaagatta ttttgaaaat
     2401 tctgcttttg tcgataataa atatttaatt gcagtaggga gggtgaaaaa agaatttgaa
     2461 tacattattc ctaacgctat aaaaaatgca gagaaaaatt taggtaaaaa atacgatttt
     2521 gcttatgatg aaagcaacga tgaatcttat tgtagtgaat tagttcgctt tgcatattta
     2581 gactcacttc aaaatccaat ttttgaatct ttatcaatga ctttcaaaaa ttctaaaacc
     2641 aacagcattg atgcttactg ggtgcaacat tttgaaaaat taggaaaacc agtacccgaa
     2701 ggcgaaccag gcacaaatcc agccgatatg agcaaatcgc cagttataga aatagtttat
     2761 aggtattatt aaaagaataa gctactttac gaactttacg aactttacaa actttacaaa
     2821 ctgttgtccg ttaataactc aaatcaaaac acttcctaaa tcaaattcat ttaattatta
     2881 ttgtgaaaaa tatttacggt gaataaacaa ctgaaaaaat tagccggtca aactgtgatt
     2941 tatggattta gcagtgtttt gggacgcttg ctcaactatt tattagttcc actctataca
     3001 cgtttgttct tgcccgaagt ttatggaatt gttactgaat tatatgctta cgtaactttt
     3061 ctgttgatac ttttaactta tggaatggaa actggcttgt ttaggtttgg caacgataaa
     3121 caaaatttta ataaagttta ttcttccata ctttttagtt taactgttac ttccagtgtt
     3181 tttataatta tagtcttagt ctttttagag cctatagcat ctgctatgca atattcagaa
     3241 catcccgaat atattttgtg gatggcactt attgtcgctt tagacgcatt tacttcaatt
     3301 ccatttgcaa gacttagatt agaaaataaa gcatggaaat ttgcaattat aaaacttata
     3361 aatatttgta ctaacatagg ttttaattta ttcttcctaa tttattgtcc aaaactatta
     3421 aattcaaatc ccgattcatt tatactaaat atttattctc caaatattgg tgtaggctat
     3481 gtgtttatat caaatttatt agcaaatatt atcacgttaa taatattgtt accagagata
     3541 tttaatgtaa aaattaaagt tgattttaaa cttctaaaaa caattttaaa atattcttta
     3601 cctttattgg tagcgggttt tgcaggaatg ataaatgaaa ctttagatag agttatgttg
     3661 aaacatttgg tgtcagaaga attaaaccct atggctcaac ttggaattta tggtgcaaac
     3721 tataaattag ctgttttaat gacgcttttt atccaaatgt ttagatacgc tgcagaacct
     3781 ttctttttcc aaaataaaaa tgaaagtaat gctcgtcaac tttatgcaaa agcaacaaaa
     3841 tattttataa tcttcggttt gatgatattt ttaggtgtaa tgttttatat tgatattata
     3901 aaatatttta tttcccctaa ttatcacgaa ggtttgaaag tcgtaccaat attattaatt
     3961 gcgaatttat ttttaggaat tttctttaat ttttcaattt ggtacaaact aaacgacatg
     4021 acaaaatacg gagcttggct tgcaatattt ggagcagtta taactattgt tttaaattta
     4081 gtattagttc caatttatgg ctatgttggt gcagcatggg ctacacttgt ttgttatttc
     4141 ttaatgacat tactttcttt ctactggggg caaaaatatt ataaagtacc ttatgaagtt
     4201 ggaaatggcg tgttctattt tgttttagct ttgagtttgt attttgttag tgtattctta
     4261 aaaccagaat ctcaaacttt aatgtatgtc ctaaatacta tccttttact tgtgtttgtt
     4321 gcaatttttg ttgttaaaga aaaattttgg agagagtttg taaaagttaa ataaaaatag
     4381 aaaatatttt aatattttta aatagggtag agccataata ataaaaaaaa ggtgcgaaga
     4441 aaaattcaac gcaccttaaa acaacctata tgaaaacaaa aaataaaaaa ctaaaaccta
     4501 tttgaatacg tatctttatt gtccccaaaa gagtcaactc cttcgtcgct aattgcgtaa
     4561 gtgcattttt cgcctaaaac ttttgaaaac attatttttt ttgcttttcc gttactgtct
     4621 atcatcttaa cacaagctaa attatcatca atattttctg cattaagatt ttcgtaacaa
     4681 attgggcaaa tgaaatttac gtggtcgcct tcttcaaaat ccacattgtc attttttcta
     4741 aaactgtaat taccaagttc tgcacttaat aatactatag cagattcgcc tttgttattt
     4801 ttagttaaaa acacaatttc attatcaata cttagataac cattgcattt aggacaaata
     4861 taatttttca tattttaatg tcttttgttg gtaacaaagg tagaaaaata ttttaataaa
     4921 acaaaattat gggaaaaatt cgttaaaatt ttgtaaaatt tagtcatcta aactcaaata
     4981 tttcattttc atttctttat aaaaatccat attcggttta ggcttggaca gctctatttg
     5041 caaagcaaat aatatttcac gtttaatatg attatttcta tcttttttat tggtttcttt
     5101 taaaataaaa ttccactctt ctttattttc aatttctttc attttatact aattaaattt
     5161 tacgcattat tattagatat ttaaaacctc gtaaataaaa attataacgt ctagctctat
     5221 tatgccaaag ccctgtatta gaagtttgat tatgtcgagt aacacaaatt aaatctattg
     5281 gttcaaaatg tttttctagt ctttgataaa gtaaaaatcc tgttggtgta aactttttct
     5341 taatccactg gtcgccaata agccatgcaa taactttatt tttttttaga actcttttag
     5401 cttcaatcat aaccttttct aactcatcat aaaactctgt tttttcacat gaaattttac
     5461 caaaacatag aggattgtca gaataattta tattgttaga ataagggctg tcaataaaaa
     5521 taaaatccac agtttcattt ttaagaggaa tttgtcttgc atcgttttta ataacgtcag
     5581 gtcttacaac gtttatatca tatcctatta ctttacgatt taattctttt gcaacatcaa
     5641 tagtcgttcc acttccacac attggatcaa caactaaatc gttttcttta gtgtatcgtt
     5701 gtaataaatt ccaaattaca aaagctggtg taacaccatt gtacttattg tttccatgtg
     5761 gcatttctcc atagttttgt gttggaaaat cccaaagtgt tgttgattca aggattaaat
     5821 catttgtttt cactttatta tcttttttaa atctgcaact aatttgtcaa acatttttat
     5881 tttatcgctt gtttcaattt ttgcttctgt cataacataa ccaccatcta aatctttttc
     5941 tacaatagct cgaccttttt taaatggttt tttcttcgtc aaagttccat cttcatctgg
     6001 tgttacaaag aagactttta tatgttttgt atttttgtca gcagcaagtt ttaatttcca
     6061 ataacctgtt tgagcaattc tttctcttaa agtaactttt actgacaaga cagcaataat
     6121 ctcacaatct ttgggattaa aaataacaat atctacatca ggcatatgca aaccaccata
     6181 ctcgctgcca aaatcaattg ctaaattgcg ttttacttta ccaagttttt ttgataaatt
     6241 agcacattca gttcgttcga gagagttgcc attaactaca tcaagtccaa ttcttttaat
     6301 ttcgtcagaa ataatatatt caatgagttt ttctaagttt ttacccttaa aagctctcca
     6361 agattgttca tggtctctgc ctttaaaatc ttttttgtgt aaagcttttg cttcttcaaa
     6421 aatttcagaa atgtgcttat aggcatcttt gccaaaagta gctttctttt tgtcatatag
     6481 ttcttgtaat tctttaaatg tcatattttt tctgtaatta agatatattc tgttgggtat
     6541 gctaatttta tatttttgtc agaaattttt ccaaatcttc ctgttttggg attgcgtgta
     6601 gaaggtaaca tttttgatgg aatctcacgc tttatcacat catgtgtttt aaaaccaata
     6661 ttatgaaatt gctcttgaaa aacttctgca ttttttattt gtacgccttt aaattctgta
     6721 tttcctatta caacgcaagc acgtccacca cgttttaaaa ctctcttcat ttctttaaaa
     6781 gtttccaaca tatctgcaaa ataattttca acttcaattt gttttttgtt tttgagtttt
     6841 gatacaattt catttgctaa attacttttt aagtctatgt tttctcgatt tttataagca
     6901 ctaccaataa atttttgacg aaattcaggc aagtcgttca aataaccaaa ccacaatgaa
     6961 ggaagttgat gtaaatcagc atattcatat gatgtaacat aaggaggcga agtaacaatc
     7021 aaatttgctt tttctgtttc gcaattcaaa tttctgctgt cgccacaact tacatttctg
     7081 tataaatcta tattctcttt tacatttttg tttaataatt tatcaaattc ataatgtttt
     7141 tttatcattt tttttgactg ttgcaaaaat aaagctaatg gatcataaac tttctttttt
     7201 ttgtcacgtg ttggttttat acttttttgt agccaaatcg aacatgattt taaaatttgt
     7261 gcaaaagcaa tcaaatagaa atcttgtaca ttcttatttt gaatttctaa aatttgtgca
     7321 aaaataacag acagtttatc tttttgttcg ggcaaaaacc aatagtctat gcgttcattc
     7381 tcaggaatat atctttgtgc tttatttaaa aaataatcaa aactgccatc aagtttgtac
     7441 ggtaaatctt gttcaaattt tgcatattca gtaagtaaat ctgaattttt tattggtgta
     7501 gttttgactt ttgacagcaa aaaagcaatt tcattaatgt cagttcctat gcctattcta
     7561 ttgtttacaa ttgattctac tactgttgtc ccgctaccca taaatgggtc aataacaata
     7621 tccccttctt tagaattttc cttgataagg cgttctgcaa gctgtggaat gaatttcgca
     7681 ggatatgtgt aatatccatg cgtaatgtaa gatgtgtctt ttgtcgtttt atctgtgaac
     7741 gagtaggaat aatcaatttc aatattgtca aaatattcgg tcattactta ttgttttcat
     7801 tcataggcaa aattaatgat tttttactaa tttacaataa ccttgtaaat aatgttgttg
     7861 ttacataaag tttaaatcgt ctgaaattat tggattgtag cagttgatgt gaactaacct
     7921 gcaattttaa agttagagtt atgtataatc gttaaaaatc aactttcaaa cttctacaat
     7981 ctaaaaaaca ttatatttgc gaaataaatt attggaaaat ttaaagatga agtgcaaaat
     8041 tttaagtttg tttttgtgtt ttcctttgct tatgttggct caacaaaagc catctcagca
     8101 atggctcgac caaaagtttt caatgtttat acattttgga ctttattctg tttatggggg
     8161 agtatatgat ggaaaacctg tagagcgtgg ctatagtgaa caaattcaat ctttcgctgg
     8221 aatttttagc gattggtatg gaaatactgc aaagcaattt aatccaacaa aatggaatcc
     8281 agatgaaatt gtgaaactgg caaaagatgc aggaatgcgt tcaattgttt ttacatctaa
     8341 acatcacgat ggtttttgca tgtatcattc tcaatacacc gattttaata ttgttgatgc
     8401 tactccttat ggaagagatt tgatgaaaga attagccgat gcttgtaaaa gaggaggtgt
     8461 tgattttgct gtttattact ctcttattga ttggaattat cctcatgctt atccaatttc
     8521 gagccataat gccgatccat tgaccgatga acattacgaa tttaacaaaa aacaagtgga
     8581 agaaattatg actaattacg gtgatatttc tgaaatttgg ttcgatatgg gttcgctcac
     8641 acctgcacaa agtaaaggtt tgtatgactt agtcaaacgt ttgcaaccgc aatgtatgat
     8701 tagtggaaga ttaggcaacg attatgcgga tttttcagta atgcccgata ataaatatcc
     8761 tgattacaaa ctcggaattc catggcaaac cgctgcttct atgtttgatg aaacttgggg
     8821 ctatcgttct tggcaaaagc gtggaagtgt tcaggagaaa gttgatgaaa aaataacaag
     8881 tttgataaaa gttattagtc gtggcggaaa ttatttactc aatatcggac cacgtggcga
     8941 tggttctgta gttgaatttg aacgcgatgt gctattagaa atgggaaaat ggataaaagc
     9001 aaattctgaa gcaatttatg gcacaagtgc aaatccattt gatcacacat ttaattgggg
     9061 tgaggttacc acaaaaaatg gagatttgta tctttttgtg aagaaagatt tttatggaaa
     9121 agatattggt atttcaggtt ttaaaggttc tattgctgag gtaaaagtgt tggaaacaaa
     9181 tgaactttgc gatttttatc ctccaaaaaa atatggtgat gattggaata tacgccttcc
     9241 gaaaacaacg tctgaactac cttattctgt acttaaagta tcttttgaaa atagttttgc
     9301 aattttgcct aaaattgttg caaatactgt gctaactcca caaaattctt ctccagtttt
     9361 tgggcattca agcctcaatt actattgcgg atataaaagt ttaattggtt atgattgggc
     9421 tttcaatact acaaaacgtc atattaaccc agaaataatg ttttctgaaa acgaacttgg
     9481 acgagaaatc attgttgaaa ttgatgaaaa caagcaaaat attacgttaa actcatctaa
     9541 taaaaaaatt gtgagattgg ttcaaaatac agtaaaatgg ggtgatttgt acagaaagcg
     9601 tagcagtatg gtttttggaa acttaacaga tttagaagga aatcccaata taatccccga
     9661 tgataaatgg acaaaaataa atcaatttaa ttacgggcaa attgttacag aggaagtttt
     9721 gtcaaaatct ggaactgtat ttttgcagga aattgaatct ataaaggagc agaaaattgc
     9781 gatagaaatt aaaagtggaa atgctcttta tatcttgcta aatggcgagt acattacagc
     9841 acatttttcg ccagaccgtg taaattctaa tcaggaaatt gtttttcttc cacttaaaaa
     9901 aggcaaaaat caattagcaa tagtctttta caatggtttt gcaaatgcgc tttcgtataa
     9961 aatcgaagct ttgcagcaat ggactgttta tacgcaaaaa ctttcatcat caaaactaaa
    10021 taaagaaaaa caatttcata ctttatcagt tcgtgatgct aatcctattt ctaatgtagg
    10081 acctctgaga atgaataata tagagattaa attttagaag atgaaatcaa aattaaacaa
    10141 taaaaataca acacttggca aatctgcttc tgtcgtttct gttatactta aaggacttgg
    10201 acaaataatg cttcaaacat atatctataa atttattgta agttactcat tgttttaatt
    10261 acagtcaatt aaaaacttaa caaaaatatg aaaaaagtgt ttaatagata tattattttt
    10321 tatttattta tcaacgacac cagataatct caaaacccag tttgtaaatt ttctaatttt
    10381 tctaattgtt cttttaatag ctgttttgtt tgtgtatctt tttctggtag atagtttttt
    10441 gcgatagtaa catctgattt taaaatacgt ccatcaggcg agtttttcca tgttgttaag
    10501 cccatgtgtt ctttattcct gtcagcttta ttataaataa tttcagctgc ggtttgtcca
    10561 gttatagcat agtgaaattt attttgcacc atggcgtaaa acgttcttgt ggtaggcgag
    10621 ttgcggtcat aatcgatgct acattctgca aaaatatcag ttatttgttg ccaaatacgg
    10681 cgttcactag cacgtataga acgaacacgt tcaagcaatt ctcgaaaata atccttacca
    10741 aacagtgttt cgccttgttt taaacgttcg tcatcaagta caaacccttt gagcatatat
    10801 tctttcagta ctttggtagc ccaaatgcga aaatgtgtag cttttgccga atttactcga
    10861 taccccacag aaataatagc atctaaattg taaaactgtg taccttgtat ttgagttttt
    10921 ccttcaatag caccgtgttg agtggttatt tccaaaatgg aaacaaccac tttttcatct
    10981 agttcgcctt cgttaaatat attcttcaaa tgtttgctta tagcgggaac ctgcacgcca
    11041 aataattgag acatagcttt ttgcgttagc catagtgtgt cgtcttttac aacagcttca
    11101 atttttacgt tttcttgagg cgtgttgtat aaaataaatt gaatttcttt cttcattttt
    11161 ttattttaat tttatcatct gcccattttt ttggattatt gcgaatgtat tccgtaatgc
    11221 ggatatatga ttgttcgtcg cgtataatat gttcgtaata attgcgttgc cataattttt
    11281 cggttagtcc caatgcgttt aattgttttg ttacagccga tttatatccg cgtacgatag
    11341 cgccaattgt ttgcgatggc gatcgtaaat gcgaacgtac tgacgaatgc aattcgtctg
    11401 tacgttgcgg cgtatcgtat tcgccaattt ccgattccga tggcgtatcg tatgccccat
    11461 tgtaggataa tttttcggtt atttcaatta tgccgtgaat atggtttggc attattacga
    11521 attcgtgtaa aataatgttg gttcgtatat ttggtgtgtt tatccattcg ttatgtgcaa
    11581 ttgtgccgta ttgattatat atcattttgc cgttttcaat atgtccaaat ctacaaattt
    11641 tatcctgcac acaaatcgtt ataaaataca atccttcctg tgaataatca tatcctttta
    11701 gacgaatgct acggcgggta tgtatgcgaa aattggtcat taattatttt ttattttttt
    11761 gccgaaaatc ggtataggta atttttgata tatttaccaa atttataaac ttttaactgt
    11821 tccttacctc tttatcgtaa tgctaccttt caaaacttgc tttttattgc tatccaatgt
    11881 gattacatac cagtaagttt cgcttggtaa ttctttgcca gcataagttc cgtcccagct
    11941 aaagtcagca cctgttgaag agtataattt tttgccaaat ctatcaaaaa tttgtacaac
    12001 agcatctgga taatggtata aaccagtaat tacccaagtg tcattgtagc cgtctccatt
    12061 tggagtgaag aagtttggaa tacttagcga ttcatctagg aaaatttctt tgcttattaa
    12121 acagtaattg tcgtccatta ttgaaattgt atatactcca gatggtaaat ttgtaaaaat
    12181 atttgaagtt tgccaagtat cgccgtcgta tgaatataca taaggttgta cacctccact
    12241 tgcaaatact tctatcattc ctcctcctga agtgtaagaa tcccaaacag gaccttcaag
    12301 aaattcaaca ttaatttctg tgcttccaga acagccaatt tcatcataaa ccgttaaagc
    12361 ataatttcct gcttgtgtta cagtaaaact agaagcatta gagccatttt gccatttata
    12421 tttaaaatat tctctatctg tttttaatat atatgtgttg ccttcgcaaa atgtagtatc
    12481 ataaccaata aaaggttcta ttaagtcatg ttcaataaca ttaattttgc ccgttgcagt
    12541 acaattattt gaatctgtaa ctgttaaaaa taaagtttga gttgaattta ctgatattgt
    12601 acttgtggtt aagttattgc tccattgata agttgcgtaa gtactgtcaa cttctattaa
    12661 atatgtttcg ccataacaaa attcaatatc attgcctaag tctaaaactg gttgtggaaa
    12721 aatatcaact tttattgtgt ctatagcatc gcaacctgtt aatgcactaa ccttaaccca
    12781 atatgttccg ctacttgtta ttgttaatgt ttcttcggta gaattatcat tccataaata
    12841 tgttaaatta tcgccttgag catttgcact taaaactaca gcttcgtttg tgcaaataga
    12901 aaaatcttct cctaagttta ttattggtga ttcacctaaa cctacattta tttcactact
    12961 tgcagtacaa gaatactggt cagttacagt gagactatat gtgccagctt gacttaacca
    13021 aatgctttct gaaaaagtgt tgttactcca atgataagaa gctaaacctg gaggtgcaga
    13081 aactaaaaca gaatctccat cacaaataac aatatttttt tctaaattga gatcaattgt
    13141 tgggaaatat acaaaagtta ttgtgtcata ataatggcaa tgagtattgg aatcaaaaac
    13201 ttttacccaa tgaattccac tattgcccga tgtaaaatat tgattaatag aggcatcaga
    13261 ccaaaaataa gaagcgtaag tatcaaaagt tgtaattgtt aactcatctc caaaacataa
    13321 agagtcggtt tcaggtaata aggcaggatt aggaattttt gtaacatttg tgctagcact
    13381 tgtgctacaa ttagtaccta aatcagtaac agttacagaa tacacaccgc cttcatttat
    13441 ttcaattatt tgactagtct cattagtact ccataaatat tcataatctc catttccagc
    13501 atttaaaact atgctttcat aagggcataa agtaatattt gttggtatag caatttgtgg
    13561 tccttgaaga gcttgtaatt ctatttttgt ttttatatag caattattct tatcaaccac
    13621 aataatttcg taattaccaa cacttaaacc acaaatattt tgagaaatta atgtgttgtt
    13681 acaataataa gtaaatggag gaacaccatt aatagtttga atatcaatac acatatcatt
    13741 ggggtcacag ttgttgcttg atattattat ttcagaaaat ccaaagccta atccatactt
    13801 tacaattaat ccatcttcat taccatttga gttaagaata tcagaattga aacttagtgt
    13861 atttgaaaaa cttcctgcca caattaactg gtttaattca tttatacaca tagcgttaaa
    13921 tgaatttgtg tcagaaacgc cttttaactc tttcttctta aaaacaccgt tattatcaag
    13981 tttagataag aaacagtcaa aatttgctgt tgaattaata ttcccatcgc cgaaatctgt
    14041 aagtccatta aattttccag ctatatatat gttttgtcca taatctactt taatatcatt
    14101 aggaataata tttccaagtc cgccaaagct tttgtttagg atttcaattc cttcaggatt
    14161 aaatttatgg attttcgcca tattattatt tagctctgat ttgtatgaaa tataagcatt
    14221 gccaaagatg tctgtggcaa cactagcttg acttgcggtg ttgctattac ttccaaaagc
    14281 ttttgcccat tgaaatactc ctgatttatt atattttgca acaaaagtgt ttaaacttcc
    14341 atttgcactt aaatttaaac ttccaaaatt gattgttcct gaaaattctc cagcaataaa
    14401 tatgttattg tctttgtcgc aagcaatact agtggcaatg tcattttgag ttgacccatg
    14461 atttgctctc cacaaaaaat ttccattagc atcatatttt gccaaaagaa aatctttagc
    14521 acctttagca attaaagtgc catttccaat ccctaaaaat tggtcataat atcctgtaac
    14581 taaaatattg tcatcagcat caacacatat atcgttacca gaatcttcgt aaaagccact
    14641 taaaaatttc ccccataaat agtttccatc aggagagtat tgaataataa atatatcgct
    14701 ttctccattt gaattgattt ggtttcctcc aatattaagt gtgcctctaa agtttccaat
    14761 aacattaatt ttgccttggc tatcaatgct aacagctttt gcaacatcat tttctgtgcc
    14821 tccacctgat tgtagccata ttaaattgcc aaaactatct aatttagcaa caaaaaagtc
    14881 gcttccacca tttgaaacaa ctgtttgaga ttgaaaattt aaacttccat gaaactggcc
    14941 tacaaccaca atatttccgc tattatcgct agctactccc aagacttcgt caaacatatt
    15001 gcttcctaaa cttttagccc aaatagaatt gtaactaatg aaacagtcag tactgtcggg
    15061 ttgagcttta agactgataa agctaaggct taataatatt aatatgtaga tttttagttt
    15121 catcttttaa aattcatcat aaaatattgc attatgaaat ttaccccatt taatgtattt
    15181 atcatggaat atttttagtc ctaagccaat ttggtgagtt ccattatgat aaagttgtag
    15241 tgaggttaaa cctatttcgt attggtagaa aatagaaaac tggtcaatag acattccaaa
    15301 acttggagct attgataggg gtgaggaatt acctttgtct aaagtatttc tgtacgatag
    15361 tccaaaccac atatcgttgt cttttctaac aaatttagat tttcttgatg atgttccacc
    15421 gtaatgaact ttaaggttta catctaactg gttgaggccg tgaaaattgg atttgaataa
    15481 acatgatggt cttaattgta cttttttatg aatttgtaaa atagagcctg catgcaaatt
    15541 tgtgagaaca tgatttattg gttctaaact tctgttatac attctgtcag tccatgggag
    15601 taagttgtcg aatgctactc ccaaaaacgt atggttgtat gagaataata ctccagcact
    15661 tgcatttggg tttacagttg tttctttgga ataagtaact attgggtcgt acatatttct
    15721 agtaaatttg gtttggtcta gtacgtgagc ccagcctcta aaagccattc caaacgacat
    15781 tataaattta tggtttcgtt tattatacaa aaatacttgg taagaaaagt tgaattgagc
    15841 tccaaatctt tggtttggtc cattcaaatc tgaaaataca tatcctccaa ttccaccttc
    15901 tctaccttta agtctatgtt tgaataaaaa taacgatgtt ttaggagcat tttcaaagcc
    15961 tatccattgt tgcctgtgaa tggctttaaa tattgaagat tgagtaagtc ctgtaaaaga
    16021 tgggtttaca agcataaaat cgaacatata ttgttcatgt acgggcaagt cttgagattt
    16081 caactctgta caaaacagtg ttgtcgcaaa tattactaat aatatgtttt taatctttaa
    16141 caactgtctt tcatttttta agttctatct ccaaaaatta tacttcctat tcttaaaatt
    16201 gtcgcacctt cttcaattgc tattttccaa tcgccactca tccccatgct tatttcacaa
    16261 aaataatcaa tatttttgaa atatttatct ttaatttcag aaaaaatttc ttttaatttt
    16321 ttaaattctt ttcttacttg ttcagtatcg tctgtaaagg ttgccattcc cattactccg
    16381 cagattttta tgttttcaaa acttttaaat tcatcagatt ctaaaatatc aatagcttca
    16441 tcataattta agccaaattt tgtgtcttcg tcggctatgt gaaattgtaa aagacagttt
    16501 attactctgt cgtgcttctt ggcttgtctg tttatttcgg ttaaaagttt taggctatca
    16561 acactatgaa ttagttcaac aaaatctgca atatatttaa ctttatttct ttgtaaatgt
    16621 ccaataagat gccattttat ttcaggattt tgaagttgat tgtgtttgtc aactaattct
    16681 tgaactttat tttctccaaa atctctttgt ccggcatcaa aagtttcttt tattgtttct
    16741 atgctgtgag tttttgaaac agcaacaagt ttagtattgt ttggaatttg ttttaatata
    16801 ttatcaatat tttttttaat ttctgacata aaaattattt tttatttcct tgaattgctg
    16861 caatagaaag caatactcct agtacaactc ctagtagagc agcagttaaa acttggtaat
    16921 tctgtggtaa aataaatgta attgtatgtg ctggaatcca aaataaagga atagtttttt
    16981 tgaacacaaa tccccattga acttcccaat ttaggtttgt aataattttt ttgataggaa
    17041 ttggttttaa aaatgcagaa acttttccac cgttattcat aatatgagtg tcggttattt
    17101 tgtgtaaagt cataaaaaca ggagcaaaag tggtgttcat cataaggctg ataaagaaag
    17161 ctccaacaag ttttaatgct gtaaaatcac cattcattgc aaaggcaatt ccgttgaaaa
    17221 tggaaaattt atctaaaaat tcaggaatcc catggcggaa aactcccatt gcaatggcta
    17281 tccacatacc aaaaaagccc caagcaattg ctcgtggtaa taatccaaaa cctttttgat
    17341 tataaacacc agttttaatt ctaagtccta tcgattcacc aagagttgct aaaatggcaa
    17401 attttataaa agccattatt aagctatact tattattaaa gtcgttgtaa aaattataaa
    17461 cttcatcaaa cacaaaaaaa ggtaagaaga agattattat agctaaaata aaaattaagt
    17521 cttgtttttt catagtttta gttttgtaat tttctttata tgtaaatcga aaatgtttgt
    17581 gttgtcggaa gaatttatga gcatatcaaa taaaatatgc tcataaatta atgatttatt
    17641 tttcaattct aattcttgca tcaaccgcct taacatattt catatttcct aacagtggat
    17701 ttaagtccat ttcaaaaatt tcaggagcag tttccactaa tgcagaaatt ctttgaattt
    17761 ggtcaataaa aatttcttga tttatacctt cttgtcctct tacaccttca attattttgt
    17821 aagatttaag gtttttaatc atatattcag cttcattttt tgaaactgga gaaaggcttg
    17881 cattaacatc atgtagaact tcgataaaaa ttccgcctaa tcctgctaat accatgtgtc
    17941 cgaatttgtc ttcgcgtttt gctccaataa acacttctgt tcctgaaatc atttcaccca
    18001 ttaaaacagc gtatgtgtct tttattttca ttaatctatc aaattcacga acaatggttt
    18061 cgttatcttt cacatttaat acaactccgc caacatctga tttgtgaact ggtccaacta
    18121 ctttcataac aactgggaag ccatgttttt ctgcaaaagc aagagcatct tcttttgtag
    18181 taacaacttt ttctattact ctgtctattc cagcagcatc aagaagttcg tagatcttat
    18241 caggacttaa atatccattt ccagcagagt caataacttt tctaattctt tctttatcaa
    18301 tttttggtag ctcaatattt tcagcttgtg gttttggagt atttaaaatt ttgcttaaag
    18361 catttccgaa agctatttca tcagggaaat ttattctacc tttttcaata aatttttcta
    18421 tttcatcttt aacattaatt atagatggaa gtattggata aataggtttt ttgctagttt
    18481 tcattttttc gtgcaataca tcatatacat cataaacagg gaaaagtccg gggctaccga
    18541 aaattaccgc cattgaatct atattgtcga aatcattatt acaagcatca ataataaaac
    18601 ctaattgttc tgctgttcct gttgctaaaa agtctatagg atttgctaca gaagaaccac
    18661 caaaaagttt gtcgagtagt tcttgagctt ttggtccttc aattgcagga acatttaaac
    18721 caccattgct aagtgcgtca gtaagcataa ctgctggacc gcctgcatgt gtaataattg
    18781 caacattttt tccttttggt gttgggtgca taaaaacaga agcaatagtt gctaattctt
    18841 ctcttccgtt gcaacgcaca ataccagctt ttctaaacaa agcttcaaca gcaacatcag
    18901 gacttgctaa agcacctgtg tgagatgatg ctgctcggct accagcttct gaagttccag
    18961 atttaacagt tgcaatttta gctccttttc taattaatga agccgcatgt ttgtacagca
    19021 ttttgggttt gttaatactt tctacgtata ataattttac tggtgctgat tttccatgta
    19081 cataagtttc gtctaaatat tttaaaattt cttcaacgcc taattgtgct gaattaccaa
    19141 cagaatagac acttgagaat tttaaacctt tagaaacagc tgactcaatt atgaaaaccg
    19201 ctgttgctcc agagcctgaa ataaaatcta tgccttgaga atcaagtttt ggaataggag
    19261 tggtgaaaat accgctataa gtttgattta aaagaccaac acaatttggt cctattaaac
    19321 ttgctttatg ctcgtttaat aaatcaacaa ttttcttttc aagtttagca ccttcttcgt
    19381 tttcttcact aaaaccggca gacaaaataa taaatccgcc agtagctttt tgttttacta
    19441 aagtttcaac tgtgtcgtaa caaaattttg ctgctatagc tataatagca cagtcaactt
    19501 gtggcaagtc gttaacatct ctaaatgctt ttatcccttg aacttcatca agtttaggat
    19561 ttacaacata taagttgcct ttaaaatcgt tgtcgattaa gttttttaaa acttttccac
    19621 ctggtttgta aacatcgttg gaaccaccaa caacaacaac acttttcgga tttaataatt
    19681 tatcgtttat catatttata ttttttatgt atttctattt ccacaaaaat ataattttat
    19741 ttcgagcata aaaataaaaa gactaaaagt tttaataaaa ttttacaatt atttttgtta
    19801 ttttacctaa gtcttgttaa atcaatcaaa atgctaagtt attttaaaat atttgtgaaa
    19861 attaaaaatt tttaagcaaa aaatgaatgg tatataatta tttattgtac ttttattgct
    19921 aaatttttgt ggctttatgg aaaaaaaagt gtttcttatt tctcgtacag atagcattgg
    19981 cgatgttatt ttgactttgc caatttgtgc gtggataaaa aataaatttc aaaattctta
    20041 tattattttt cttggaaatt catacacaaa acctatagtt gaatgttatc cttatgttga
    20101 tgaaataata gattggcgag aacttaaggg gttagataaa aaaacacaaa ttaatatttt
    20161 aaaacaaaaa aatattgatg tttgtattca tgtttttcca aaaaaagaaa ttgctaaatt
    20221 ggcaaaaaaa cttaaaatac caacaagaat tggaacttcg catagagttt ttcatttttt
    20281 tacttgtaat cttagaccaa atttttccag aagaaaatct gatttgcatg agtctcaatt
    20341 aaattttgaa ctattacgtc cattaggttt ggaaaaaatt ccgagtttgg ctgagataca
    20401 agaatatttg tcttcattta ttattcctaa agtgcattta gattttgatt tgtctgcaaa
    20461 aaataaaatt atacttcata ccaaatcaaa aggcagtgca gtagagtggc ccatgaacaa
    20521 attttttgaa ttagcagtaa aattagcaga tttggattat gaggtttatt ttacaggaac
    20581 agaagcagaa gctgtccatt ttagagataa attgccaaag catcaaaaaa ttcatgatat
    20641 gtctgctaag tttacactta cagagtttat ttactttatt tctcaatgcg aagtaattgt
    20701 tgcagggagt acaggacctt tgcatattgg tgcggtaagt ggaataaaaa ctataggaat
    20761 ttattctcaa acacgaccaa ttcatcaagc tagatggcga ccaataggga aaaatgtttc
    20821 tattgtaaca aataagcaag aaccagaaat cgggaaaata aatcctcaat atttaaagga
    20881 tattgaagtt gacgaagttt ttaaattggt tatagcagat aagtaaaact ttattcttgc
    20941 ctttcaattc caagactttt agctttacta atcatatatt cccaagcttt ttctttatca
    21001 ttaggaattt cgccgtcaag aatagcatct tttatagcat ttttaattat tccaacagtt
    21061 ctgcttggtt caatattaaa gtagcttata atttcatctc cgctaattgg cggttggaag
    21121 tttctgagcg aatctctttc ttctacttct acaatttttt gtcttagtgc tttaaaattt
    21181 gttaagtatt gctttacttt tttttcgttt tttgaagtaa tatcagcttc acacaaagtc
    21241 aataaatcat ctaaatcttc gccagcatca acaattaacc ttcttattgc agaatcggta
    21301 acttcatctt caactaaagc tattggtcgc atgtgcaaat cgactaattt ttgcacatat
    21361 ttcatttttt catttaaagg gagttttagt cttgcaaaaa tttcaggcac cattttttgt
    21421 ccaataaaat tgtgtgcgtg aaatgtccag cctatacctt cgacatattt ttttgtttta
    21481 ggttttgcga tgtcgtgaag taatgcagcc catcttaacc ataaattgtc gctattttct
    21541 gcaacattgt caacaacttg aattgtgtgc aaaaagttgt ctttgtgttt tacacctgct
    21601 tgagtgctaa caccagctaa attgtataat tcaggaaaaa taatttctaa aagttcagaa
    21661 tcgaatagta gttttaaacc aatagacggc tgtttgctaa gcaaaatttt atttagctcg
    21721 gtatgaattc tttctgcact aataatttca attctatgtt tatttctttt aattgcttca
    21781 aaacactcgt catcaattat aaaatcaagc tgagatgcaa atctaattgc tctcatcatt
    21841 cttagcggat cgtcagaaaa tgtaatatca gggtctaaag gcgttcttat tgttctataa
    21901 tgcaaatctt caattccatt aaaaggatcg gtaatttcgc cgtaagtttc tttgtttaag
    21961 cttattgcta aagcatttat agtaaaatct cgtctttttt ggtcgtcttc aatgctccct
    22021 tgttcaactt ttggattacg actttcggga gaataagatt cttttcttgc accaacaaat
    22081 tctaattctg tgtcttcaaa ctggaacatt gctgtgccat aggtcttgta aatgcttagc
    22141 ttttttattt tgagcttatt agcaagtttt tgagcaaatt caattccatt acctttaact
    22201 actatgtcta tatctttttt atcagctctt tttaaaagtt tatcacgcac ataaccgcca
    22261 ataacataga cttcagtatt gtcagtttcg tcaaaatcgc gaattaattt aaatatttta
    22321 tgtgttaaca attccgaaat attattacta ttctttacca cttgaacaaa aacttttaaa
    22381 ttttgttata aattcagtta caaaaatatt taaaaagatt gagaaaatgg cagaacattt
    22441 aacaaaagaa aaatttatcg aaagaatttt cgattatgaa aatgaaactg agtggaaata
    22501 taaaggtgaa agtcctgtgt ttattgactt ttatgcagac tggtgtggtc catgtaaaat
    22561 gatagcacct gttgttgaag agttgagcaa agaatatgaa ggtttaatag atatttataa
    22621 agttgatacc gaagaccaac aagaattggc atcaattttt gggataagaa gtattccttc
    22681 tttgttgttt attcctttaa gtggacaacc acaaatgatg actggtgcag ttccaaaaga
    22741 cggtcttatt cagatttttg aagatgtttt taaaataaaa aaatagtttt ttttgtgtta
    22801 gtgatttttg gtttttagag tccaatccga cttatttatg gtgctagtga atttgcttgg
    22861 attggacttt ttttatgttt ttaaactttt ttagacattt tttcgtttta aaatcttatt
    22921 ttcgcaaaaa tattttatgt tatgaataca cattttatta caacagaaca tttgagttta
    22981 gacaaactac aagaaatttt taaaaacgga tacaaaatcg agctttccga acaagcaaaa
    23041 aacaatatta ttaaatgtcg tacttatctt gacaataaaa taaaaactca tgataagcct
    23101 atttatggta taagtacagg ttttggttcg ctttgtaata caaatattgc ttcagaagat
    23161 ttagaacaac tacaaacaaa ccttgtaata tctcatgctt gtggaatggg agcagagata
    23221 gactcagaaa ttgtaaaact aatgatgtta ttaaaagttc aggctttatc ttatggaatt
    23281 tctggtgttt gtcttgaaac agttgagaga ttagtgtatt tttataataa tgatataaga
    23341 ccagttgttt acgaattagg ttcattaggt gcttcgggag atttggctcc attagctcat
    23401 ataacacttg cccttattgg tttaggagag gttaattata aaggtaaacg acaaaaaact
    23461 gaaactgttt tatcagaatt aaatttaaaa ccattaaaat taaaatctaa agaaggttta
    23521 gctttaatga acggaactca gtttatgttg gctcatgcaa ttacaggtgt ttggcgtgcc
    23581 gaaaaaatta tgtttaatgc cgacagaatt gcttctttat ctttagatgg atataatggt
    23641 ttgatagcag ctttttaccc aagtttacat acaattcgtc cacataaagg acaaattgaa
    23701 actgcaaaaa atataagaac attcttggaa ggttcagaaa ttgttgcaca aacaaaaact
    23761 catgttcaag acccatattc ttttagatgt attcctcaag tgcatggtgc gtctaaagac
    23821 actttaaact atgtaaaatc tgttgtggaa actgaactca attctgtaac tgataatcca
    23881 atggttttcc cagatgaaga tttggtttta agcggcggaa atttccatgg acaagcttta
    23941 gctttagttt tagacttttt gtcaatagca gtttctgaaa ttggaaatat ttctgaaaga
    24001 cgtatatatc gtttgattag tggcgataga ggtttaccag cttttcttgt tgcaaatcca
    24061 ggattgaata gcggttttat gatacctcaa tatactgctg cttctatggt tagccaaaac
    24121 aaacaacttt gtactccagc aagtgtagat tctatttcat caagcaacga acaagaagac
    24181 catgtgagta tgggtgctaa tgcagctaca aaattgcata gagtgattgc aaatacagaa
    24241 agaattatag gaattgagct ttttgcagct tcacaagcac tttcattccg cgaaccatta
    24301 aaaacatctc cagaacttca aaaatatctt aacaaattta gaaaaattgt acctttggta
    24361 aaagatgata ttgtgatgta tgaaatgctt cataaagcag aagaatttgt aaaatcagac
    24421 gaatagttta ataggctata aacactatac ggttaaaaat tttggaaaat aaatacttac
    24481 aaaattttta ttgtcttctt aattcattaa atttgctgta attttaaagt aaattttatg
    24541 aaaatcaagt ttataggagc tgcacaagaa gttacaggta gtaaacatct gttgacttta
    24601 gacaatggta agaaaattct tttagactgt ggtatgtttc aaggcaaagg aatggaaact
    24661 gatagaatga atcgtaattt aggtttcgaa ccaaaagaaa tagaccatat aatattatca
    24721 catgctcata tcgaccattc tggattaatt ccatattttt atactcaagg tttcgaaggt
    24781 tcggtaattt gcaccgatgc aactcgaaat ttatgttcat atatgttacc cgattctgga
    24841 tatattcaag aacaagatat aaaatggtat aataaaaaac ttctaaaaca aggattgcta
    24901 ccaattgagc ctatttatag acattctgat gctgtaaaat caatgaagct ttttataggc
    24961 gttgcaatgg atagaaaatt tagaattgat gagaatatta cagttaaatt tacagatact
    25021 ggacatattt taggtggagc agtagttaat ttggaaatta aggaaaatgg aaaaactaca
    25081 aaaattgctt acactggaga tattggaaga ccttataata aaattattaa accgccagca
    25141 gcttttccac aagcagacta tttaataaca gaatcaactt atggcaatag attgcatgag
    25201 gataaagctg atgctgaaga aaagttattt aatgttattg tggacacttg tgtaagaaaa
    25261 ggcggaaaat tgattattcc ttcatttagt gtaggaagaa cacaggaaat agttctttta
    25321 atgaataatt ggtataacga aggaagatta ccaaatattg atgtttatgt tgatagtcct
    25381 ttgtcggtaa atattacaga agtttttaaa atgcattcag aatgttataa cgatgaagtt
    25441 ttagaagttt taacatacga cccagatcca tttggctttg aaggtttgca ttatataaaa
    25501 aatgttaatg cttctaaaaa actaaatgat acgacagagc catgtgtaat tatttctgca
    25561 tctggcatgg ctgaagcggg tagggtaaag catcatattg ctaatagtat tagtaatccc
    25621 aaaaacacaa ttttacttgt tggttattgt gccccaacaa ctttaggtgc gagattgcaa
    25681 gaaccaggac ttaaaaaaat atctattttc ggtacagaac atgaagtaag agcaagtgtg
    25741 aaacttttag aggcttttag cggacatggc gattatgtag aaatggcaga atttatttca
    25801 tgtcaagata aagatttatt gaaaaaaata tttttagtac atggcgattt agaatctggt
    25861 gaacattata gaaaatatct tttaaaaatg ggatttaaaa atatagaaat accaaaaccc
    25921 ttcgatgaag ttgagctagt ttaaagattt aggttttact tttacattat ttctggaact
    25981 tctattccta ataaatccat tgcagatttt atagtttgtg caacagtttg tgctaacaat
    26041 agtctgagtt gtagcgtttt agattctgtt tcttttaata tgctatgctc atgataatat
    26101 tgattaaaaa gttttgttaa agcataagta taattagcaa taaggctggg gtcaagtcta
    26161 tttgcagatt cttgtataag tttttcaaaa gtgcataaat gagaaattaa ttcaatttct
    26221 aagtcaacaa tatctgcttc ttcacttgct ttaccatgaa acttgatatt cattttttca
    26281 gcattgcgta aaatagactt tatgcgtgca tgtgtatatt gaataaatgg tcctgtattt
    26341 ccgttaaaat caatagattc ttttggatta aatattattt gttttttagg gtcaacttta
    26401 ataataaaat attttaaagc tcctaaggca atcattctga tgattttttc tttttcatca
    26461 tcttctaaat catgaatttt accagcttcg ttagatatgt ttctagctgt ttctatcatg
    26521 tcgctcatta aatcatcagc atcaactact ttgccttcac gcgatttcat tttgccctcg
    26581 ggtaattcta ccattccata agaaagatgt tcaattacat cgctgtaaga tttatttatt
    26641 ttagaaataa tcttttttaa aacggcaaaa tgataatctt gctcatttcc aactacataa
    26701 attatttttg aaggattaaa ttcttcaatt cttttgatag cagttccaat atcttgagtc
    26761 atataaactg ttgttccgtc actgcgtaaa agaattttcc tatccaaacc ttcatctgtt
    26821 aaatctatcc aaaccgagct gtcttcatct ctaacaaata tttcgttttc taatccttgt
    26881 tttacaatgt cttttcctaa taaataagtg ttggattcat aataaacttt atcaaaacta
    26941 attcctaaat cagaatatgt ttgctcaaaa cctttgtaaa cccaagagtt catcattctc
    27001 caagttttgc gaatttcttc gtctccgttt tcccattttc taagaatttc gtgagcttct
    27061 aaaattattt cagcattagc ttttgcttgt gtttcagttt gtccagcttt aataagttct
    27121 tcgatttgtt ttttatattc tgcttcaaat tttacataat aatcaccaac taatttatcg
    27181 ccttttttgc cacttgtttc gggagtttcg ccatttcccc atttcatcca agctagcata
    27241 gatttacaaa tatgtattcc tctgtcgttt attaaatttg ttttaataac atgattgcca
    27301 gaggctttta atattcttga gacagaatca ccaagaatgt tgtttctaat atgtcctaaa
    27361 tgtagaggtt tatttgtatt tggagaagaa aattcaacca ttatggtttc cttgtcaaat
    27421 tcttcagatt ttttattgtt taaattcaaa ctgtttagtc tgtctatcca atattcattg
    27481 gtcattctaa aatttaaaaa accgtttata acattatatg acgaaacatc atcatatttt
    27541 tgtttaataa aagcacctaa atctttagcg gtatcggcag gcgatttttt actaaattta
    27601 gaaaaagcaa aaacaactac tgtaaagtcg ccattaattc cttctttagt tttttctacc
    27661 gctacatcaa tgttggttgt atcaacagaa tacagagaat taagagcttc ttttatatat
    27721 ttttttatat taatttccat aatttaaaaa tcaactgcaa aaataaaaaa aaaaacaata
    27781 aaaaaactaa gatttatcat tttgttgata aaaatcacaa tatatagaaa ttccgcaagt
    27841 tttacatttt ggatttcttg ctgtgcatac gtatctacca tgtaaaatta accaatgatg
    27901 tgcatctgga atatcttttt caggaatatg tttaactaat tgcttttcgg tttgtaaaac
    27961 agtttttgca ttttttgtta aaccaattct tgccgaaact ctaaaaacat gagtatcaac
    28021 aggcatttta ggttgtcgaa ataaaaccga agcaataaca tgagctgttt ttctaccaac
    28081 accgggtaat ttttgaagtt catcaacatc ttcgggaact tttccatcat atttttcggt
    28141 taatatcttt gctgtttgag caagatattt tgctttgttg tttggataag tacaagattt
    28201 aatatattca aaaattactt ctggactagc aaagctcata gtttgagcgt ctggataatc
    28261 attgaataga gcaggagtga tgagattaac tcttttatct gtgcattgag ctgacaatat
    28321 tacagctact aataattgaa aatcgttttg ataatcaagc tcagtttcgg ctaattttat
    28381 attttcccga aaatagtcta aaacatattt gtatttttct tttattgtca tagtgccaaa
    28441 aaaatgacaa catcttataa aaacttagct aatgtattac tttagatatt gcagtttaaa
    28501 tatcttagta tcagaattta gcatttataa gatgctgtca aattttatgt tgttttaatt
    28561 ttcagataaa aatctttctg catctattgc ggctttacag ccagaacctg cagcagtaat
    28621 agcttgtcta taattagggt cttgaacatc accgcaggca aaaacaccgg gaacattagt
    28681 tttagaagtt cctggttttg ttttaatata agattgctcg tcgagctcta tataatcttt
    28741 aaataggcta gttgcaggag tgtgtccaat tgcaaggaaa aaaccatgaa tatcaatatt
    28801 tttttcttcg ttggttttgt tatttcttaa tctagctcca gttactccag aactatctcc
    28861 aagaacttct acagtattat gattccaaag tatttcgatt tttggattat tcataacttt
    28921 ttcttgcatt gcttttgaag cgcgtaaaac atcgcgtctt acgatcatat aaactttatt
    28981 gcaaagtcct gcgagataac tagcttcttc agcagcagta tcgccaccac caacaacagc
    29041 tacatcttta tttctataaa agaaaccatc gcaagtagca catgccgaaa cgcctaatcc
    29101 tttaaacttt tcttcagatt ctaagcctaa ataattagca gtagcaccag ttgcaataat
    29161 tacagtttct gcttcaattt catgaacatt gtcggcaaca actttaaaag gtcttttact
    29221 aaaatcaact tttgtaatac aaccaaatct aacatctgtg ccaaatcttt gggcttgagc
    29281 ttttagttga tccatcatta taggaccaga aactccttcg ggatagccag gaaaattatc
    29341 tatttctgtt gtcgtagtta attgtccacc tggttgtaaa ccttcgtaaa gcacaggatt
    29401 caaattagct cttgcagtat aaatagcagc agtataacct gctggaccag agccgattat
    29461 caaacatttt attttctcac tcattgtaaa attattttat atattattat ttttgaattg
    29521 tgataggaat ttcttcataa actaaaacac acataccatt ttcagtatta caagcctgac
    29581 cgtcaataat tatggttagt gagtttattt cagtttttgg aacataatta attacaaatt
    29641 cactgttgcc ttgatatgag ctaacattca tggtaaaaac atcatcatat acagttattg
    29701 caggtgtcac ttctttgatg tcagcattat aaaccatgtc ctctttgtct tttatatcaa
    29761 gttttaatgg taaaggaccg ccttcatcta gattcattcc gtaaacatac caattgtttt
    29821 taatatcagc tttaatgtga aaattaaaag aaccatcttc tgaacgcgac tggttaatgt
    29881 tccatgtaac aggattttct tgagagaaag ctgacaagct catcaaaaaa acaaaactta
    29941 ttaatgttaa aattttcata gtaaaaatat tttttataat aatttcataa atatataact
    30001 aaaaagacta caaatagttt gcccgaaatt aatatttttt ttaattctaa ataaaaattt
    30061 gttatttttt ttgaatttac atatggtttt gaaagaaaat ataaataata attattttat
    30121 ggaattaata tcaaagttgc actatctgtg atataaacat ttgtaaaatg agttccagaa
    30181 cctatccaat gaccattagc tggactgcca taaatatttg attttactga tgcagggttg
    30241 gtaaaaggat tgcttcctgt taaaacattt ctgtaaacag aactccaaaa attaaaacta
    30301 tttgcatcaa ttgctgacat ttttaaactt acactgtcgc caactttaaa gcaaactaaa
    30361 cttagaaaat ctaaatcttc atctaaccaa acattatgat agtaagcatt tcgttcataa
    30421 cctttagtta atggtgtgta agaaaattct tgaccattta ataatttatc gtctaaaata
    30481 tgtgagccaa acgaaggacg ataataccat ttttgttgtt ctaagacttt aacataaaag
    30541 gtgtaataat ttccaaattt ttctgggtca gtaaatttaa agcctaaaaa acccattgta
    30601 tcatttcctg gcaatagatt aaaccataca gaatcaatag gtacaggctc aggtattgtt
    30661 gttgttgagt tatattcctt atcttggtat aaaattttta aatcatatga tttgttaacc
    30721 tcgcctttaa attttgtgcc acggtaaccg tgatatgggt atctttccat aattgtaggc
    30781 tttaaagtgt cggtaatagc tccgttagaa acaattacgc aagcatctga acctttaaca
    30841 attgaagcat ttagtattgt tgtatcaata ttagtaaaat aacttgaatt ttttgaaata
    30901 aaaactattg gataatcatc agaatctatc attgcttcta ttacaagttt ttcgtcagct
    30961 ttaggtaatt ctaagctaat atcttttatg caagatgaag ttatgattat taaaagtaaa
    31021 aatataatat ttctcagttt cattttcata aattaaaatt taaaattcca agttattgat
    31081 ggtaaaatag gaattaaaga aatttgttta gctgttgttg taaaatcgcc agtttcaaca
    31141 gaacctttat cttctaaata aataaaatat ggattttttc tattatacac attaaatatt
    31201 gaaaagttcc aagaagattc ccatttgtcg gtttttttgt gttgatatgt tacggaaaga
    31261 tctaaacgat gatatggatc cattctgtaa gaatttcgtg gtccatattc gcttatcaaa
    31321 tcaccgtcta tcatatatct tgctattggt aaagtagttg tatttcctgt tgcaaaaaca
    31381 aaaactaagg aagcagtcca tttttcagta atttgataag ttgttgttaa agacaaatcg
    31441 tgtcggcggt catattttgc aggaaatggt tcaccaccgt ttatatcatc aaaaattctt
    31501 tctgttttag accaagtgta accaatccag cctgtaaaac gtccagttct cttttttata
    31561 aacaattctg ctccgtaaga ataaccatct ccaaaaataa aataattatc tggattgtcg
    31621 tttacatcat tgccgggtaa agcaccatcg gcatattcaa ttaggtcgta aagacttttg
    31681 taataggctt cgacagaagt ttcgaaatgg tcgttaaaaa aattcctgaa atatcctatt
    31741 gcatattgac gaccgccctg tggttttaca acatcgctac aaggcaccca caaatctgtt
    31801 ggcagggtcg aagccgacaa agaagctaaa tgaataaatt gtttattttg cataaaagat
    31861 gcctttacag aagaattttt gtctaaacta acttttatgc tagctcttgg ttcgtaagag
    31921 tcgtaaaatg caacagtttc tccttttcca tatgttatag tgtctatatt attcattaaa
    31981 ttctcgtctt taacatatct tacgaatttt ccaacttgat taaacattgc agctctaaaa
    32041 ccaccgtaaa tagtaacttt gtcagaaatt ttaaaatcgt ctccaatata aattgcagca
    32101 tcgtgagctt tttgtttttg tacagatgaa aaatcatact cagtttcgcc taattttgca
    32161 tttacagaat taggagtaaa aatatgataa atataattta acccaaattt aatcgtgtgt
    32221 ttaggatttg gtagaagtgt gaaatcttgt ttaatgtaat aatctctaac gccagaattt
    32281 tgaataaatc tcatgccttc attgggtttg tcttcatcgc ccatttctat tgtagtttga
    32341 aatttataat ctgaggctac taaagttgtg ttggagaaaa atttagaatt aaaaagatga
    32401 ttccacctaa tgctaccagt tccatttccc caaggaattt ttacgttaaa atttgcttct
    32461 tttgatctaa aatcaaaaat atcttttccg taatatgcgc taagatataa tctgtctttg
    32521 tccgaaaatc tataattaag ttttgcattc aaatcataaa aaaagtagcc cgaaccttta
    32581 aatggagatg tctcttttat aaaaggctgt attagtatat ctgcataagt tctacgacca
    32641 gaaatgataa aagatgattt ttcttttaca ataggacctt gcacagtaag tcttgatgca
    32701 atagttccaa ttccaccttc tacttgatat ttgttcatat ttccattttt cattgaaata
    32761 tcaagcacag agcttatgcg tccaccatat tctgctggca ttccaccttt ataaatttcc
    32821 atgtctttta ttgcatctgc attaaagaca gagaaaaagc caaacaagtg agcagcgtta
    32881 tatacagtag cctcgtctaa tagtactaaa ttttggtcgg gacctccacc acgtacataa
    32941 aaaccagagt ttccatcgcc agcagattga atgccaggaa gtagctgtaa agtttttaaa
    33001 atatctactt cacccataaa tgcaggaagg gttttaatcg tttcgacagg caattttatt
    33061 acagacattt ttgagtcttc aacgtttttg tttttttcgc cactaacaat tacttcttga
    33121 gaaattatag cagaagtttc taaagagata tttaattttt tatttgaatt taattttatt
    33181 ttttcaacaa cttcttcata gccaacaaaa gaatatatta tttcatattc gcctgcagga
    33241 atagaaagtg agtaaaaacc ataggcattt gttgttgtac caatgttttg gtctttaacc
    33301 attatgtttg cacctaataa atcttcgcct gtgtgtttgt cttttatata gccactcaca
    33361 gtatattttt gagaaaacaa aggcatacca ataaatataa aaattgcaaa atatattaat
    33421 ttaagcttca ttttttttga ttataacgta tgcaaatata tctaaacttt ttatttattt
    33481 acaagcaaaa tttagatttt taaatatgtt ttacaagcac taataatcat caatcaaaat
    33541 catttttttt attatttttg cctcaaaata aaactaattt taaattttac tgtttattga
    33601 ataagataaa aatataaaaa aatgacaaag caaaaagtag ttttaatgat tttagacggc
    33661 tggggcaagg gaaatggaca aaaagccgat gttattagta atgcaaaaac accaaattac
    33721 gataaattta taaaaaactt tccaaattct gaattactta ctgacggaga aaatgttggt
    33781 ttacctaaag gtcaaatggg aaattctgaa gttggacatt tgaatattgg tgctggacgt
    33841 gttgtgtatc aagatttagt taagataaat aaagaaatag aaagtggtaa gttttttcaa
    33901 aatccagttt tattaaaagc ttttaaatat gcaaaagaaa atgataaaca agttcatctt
    33961 ttaggtttag tttcaaatgg cggagttcac tcaactgaag accatttgct tgcaatagta
    34021 aagctttctg atgatttagg cttgaaaaaa acttttattc atgctttaac agacggtaga
    34081 gatactgacc caaaaagtgg aattggtttc ttaaaaaaag ttacagatgc tttagaaaat
    34141 tccaatgtta aactagcaag ccttgttggt cgttactata caatggatag agacaaaaga
    34201 tgggagagag tgaaaaaagg ttacgacttg atgttggaag gaattgggaa aaaatcaact
    34261 gatattttaa aatcaatgca ggaatcttac gacaacgaca ttacagatga gtttatagaa
    34321 ccaatagtaa tgaccgataa agacggaaat cctgtaacaa aaattcaatc tggagatgtt
    34381 gttatttgtt ttaattttag aaccgataga ttaaggcaaa ttactacagt tcttactcaa
    34441 aaagatatgc ctgaacatgg aatgaaaact cttgatttag agtattttac catgactcgt
    34501 tacgacgaaa gctttaaggg tataaatgtt atttatgaaa aggataattt aaacaaaact
    34561 cttggcgaag ttattgctaa aaatggttta agtcagctta gaattgctga aacagaaaaa
    34621 tatgcacatg taagtttctt ttttagtggt gggcgtgaag aaaaatttga aaacgaaacc
    34681 agaattttaa ttccttcgca aaaagttgca acctatgact tgaagccaga aatgagtgct
    34741 ccagaagtta aaaaggcgtt aatggctgaa attgaaaaaa atattcataa tttaatagtt
    34801 gtaaattttg ctaattgtga tatggtcgga catacaggag tgtatgatgc aattgttaaa
    34861 gctgttgaaa ctgttgatac tgcaatgggc gaagtggttg aattagcgat taaaaatgat
    34921 tatcaaattt taataattgc cgaccatgga aatgctgaca atgctcttaa tgacgatgga
    34981 acaccaaata cagcacattc attaaatcct gtaccttgta ttttaataaa ttctgaatat
    35041 aagaaaattg aaaatggagt gttagcagat gtggctccaa caattttaaa aatgatgaat
    35101 ttagctattc ctagcgaaat gacaggaaaa attttggtat aaaaataaaa taataaagca
    35161 ggctattaat aaaatttttt ataaatttgt cataaagaaa gtttatgctg aaaattttag
    35221 gtttttgtgg aaaaataata atcacagtag ccgactggtt ttataagcct tttagttcat
    35281 atattccaca agttacgttt agatatggct ttactggagg tttaaataca gttttagata
    35341 tttttctgta ttttgttttc tacaactatg ttttccaaaa acaaaattta gacttgtatt
    35401 ttgtaactct cagtcctcat attgcagctt ttgcttttgt ttttcctatt acatttacta
    35461 caggattttt attagcaaaa tatgtaactt ttactcaatc gcaactaaga ggaagaaagc
    35521 aactttttcg ctatggactt acagttatag gagcaatttt cttaaactat attttcttaa
    35581 aattctttgt tgaatattgt aatatttggg caacattatc aaaaattcta actacgtgct
    35641 tagttattgt atattcatat tttatgcaaa gatatttttc ttttaagaca acgcctgtaa
    35701 aggaatgaaa ttagtattat tcttctaaat aaattttgaa tttacttatg tttaaagagt
    35761 ccatttcagt attccaaaaa taaactttta atacatcaga gttgagaatt tttgtattga
    35821 tattgtgagt tattgagaaa agaccattat tatgaatagc ctttaaatct tcaatattat
    35881 aggctttcca aaaatctcca cattgaatta ccaacaatat tttattttca tgactaatat
    35941 tgctatcatt gttaataatt gcacgaattc taagtttagt aaaattattt tttttaatat
    36001 cgtcaatagg aaattcatga gtatgactaa attcatcttg agggtttaca gttaggctta
    36061 gggtagttga gtcatgtgaa aattggaatt tctcgaaatt taatgaatgg tctataaata
    36121 tgtttttatt tgtatgaact ttcaataccc atgcatacca agtgttgggt tcaggtattt
    36181 ctaagaaata aagagttttt gttcctttat ttatacttgg tatgtatatg gtttcttggt
    36241 cgctagatga aataaaattt tcaacccatt gcacttgttc agattctatt tttgctctat
    36301 gtgatactat gtcaacataa gcatctttta agttttcaga tgatgtgata ttgaagataa
    36361 gacctaatag cagcaaagca taagataaat ttatattttt atatctttct ttaatataaa
    36421 aaacatttcg agaatttaat aataagcata aaaatagtat taatgcaata gtataacctg
    36481 gaataattcc tctatatctc atagggtaag tcaagtgtcc taaagaaaat tttaaaatga
    36541 aaacactaat aaaaacagat aataatattg caactacaag taaaatagtt tttgtgccag
    36601 cgtattttgt ttgttcattt tttcttggat aggttaaaat tgctacaatg ataagtaaaa
    36661 atccaaaatt aacaaaccac ttgctaatca agctcatgct ttcaaatgtt gacattatgg
    36721 cagaacctaa aaagtcgcca ttgtcaaaaa cttgagattc gttatataat ctatttgtat
    36781 ttccaggagc ttttataaaa aaataagtta aaactaaagt acatgcaatt ataatatatg
    36841 tagatagttt aaattttctt ttgtttatta attcaaaaat aacaaatcct aaaattgtga
    36901 aaaagcaaag tactacccat gcttcgtgag atcctattgt gaaaaataaa caaacgatta
    36961 acgcaataat tactagtggg ttattatttt tattaaaaaa acttataagt aagccaagca
    37021 ttagtagata taaaattgct ggtaagatat aaacacttga cccagagaac caataaaatt
    37081 cacgaataga tggaataaaa caaatataga aagaaacaaa agttaatgtt attaaaattg
    37141 aattttttat attaatgttt ttaaaaatgg agcgaactaa aaagtatgca gaaagtagta
    37201 aacataagaa acttaaatgt gggattgttc tagcaaacca tacgttcata taaggtcgtc
    37261 caggaagcaa tagtaatgca gaattagcat atctgccgtt ataacctgtt ttataccatt
    37321 cagaaattgc atcaaaatac gaaaattctt taaatacatg agagttcagc atatcgtcgc
    37381 tagcacccgg caaattgtat tgaataagat aaataaatgg aaaaattgag aaaagcaata
    37441 agcaaataat taaggcatat agcaaatttc cattatttat ttgatttttt attttactaa
    37501 ataaactatt aggcttcatt tgtcttagaa tttttaatta tataaatagg tctgtctttt
    37561 atctcggata ggcttttgcc aaaatatatt gataaaatag caattaagaa acttagaatt
    37621 gcaaatccaa tactttgtga aataattatt gttgtgtaac cagatggtat tttgttttct
    37681 aaaaaatatg aaactaaact aacaataata agaattattg aaaataaaaa atataacatt
    37741 gatatgatta cagatattct taatggtttt tctgaaaaag caaatattgc ggtaaaggct
    37801 aatttgaaaa gctttttaaa attataattg cttgttccag caattcttgc actagcaaca
    37861 taatctaaac ttgttatgtt gaaaccaata atttgtatat agcctcttaa aaaacgattt
    37921 ctttctctaa aattatttcg taaaacattt gcaacatctt ttgaaattaa gaaaaaatca
    37981 gaagcatttg gctcaaagcg atgaccagat aggaaactta cagttttata aaataatgaa
    38041 gaggataatt tgcccattgc tttttggtct tcacgatttg ttctgttcat aagaataatt
    38101 tcggcagatt tttcggtttc tgcaagcata tcttctattt tacttggcgg atgttgcaaa
    38161 tctgcatcca tacaaattat aaaatcagat tctgaattgt caatacctgc aatcatagct
    38221 gcttcatgac caaaattacg agaaaattct atgcttttta tctttagcga agtttcgttt
    38281 tcaataattt gatttatgat tttttggcta ttgtctttac tgccatcatt tacaaaaatc
    38341 acttcaaaat tataattttc attaatttta agtaattctg attttaaaac agaatagaat
    38401 tcgtttaagc cttcttgctc attgtaaaca gatgcgataa ttgatatgtt ttttttcact
    38461 gctatatttt gtaaattaaa taataaaagc aaaaatagtt aatattttgt aaacaactaa
    38521 tatttcgttg tggtaaatta aaataattta tgtaaattta tgaaaatttt aaaaatcaaa
    38581 acaaatgaag aacagactta gtattcacaa cggcgatatt acaaaattag aagttgatgc
    38641 aattgtaaat gctgctaaca aaactttatt aggtggaggt ggagttgatg gtgctattca
    38701 cagagcagct ggtaccgaac ttttaaacga atgtcgtaaa cttaatggct gcgaaactgg
    38761 agaagcaaaa attacacttg gatataactt gcctgcaaaa tatgttattc atactgttgg
    38821 tcccgtttgg agaggtggaa ataataaaga agctgaatta ttagcaaatg cttatataaa
    38881 ttctttgaaa attgctgcag aaaaacaatt tgaaactata gctttcccct caatttcaac
    38941 aggtgtgtat tcttttccaa ttgataaagc aactaaaatt gcagttactg aaacaatcaa
    39001 atttttagaa caaaacaaat tcccagaaaa agtaatattt tgtgtctttg gaaaagaagc
    39061 agaagaaatt tataaagaaa tgtttttgaa tttaaacttg tgattgtgtt agtttttgtt
    39121 ggaaataata tcttaataaa atgaatgtta tttaaaacac atcgtcccaa tcatcgccat
    39181 tatcaacaaa ggacgctttt ttcatataat attctttttg atagcctgtt ccaaaatatt
    39241 gaataacaaa aaatgcagta tcactcattg attttatttt aaaaagactg cgactattgt
    39301 atttgtcata gcctacataa tatttaggag cgtggtcgta ataaattttt aattctgttt
    39361 ttttaatttc ccaagcaaaa ctatcggctt tgcttacatc gcttaaaaaa gatattcctc
    39421 tgcctttttc gtcaaattta tagttaggca tatcaaaaat tggcttatta tcagtgcctg
    39481 taggtgtcca atagcctaaa atttttgttt cccattctaa atctgtttca gtctttccac
    39541 aaaatgtaaa tataaatgtg aaaattaaaa tcattcctaa aatagaatat tttaatattt
    39601 tgtctttagt tttcattatc ttcattatat tcattatcaa tatcaactac actatcttta
    39661 ctagctttat catcttcttc atctttatcg ctttttgttt tgttagaaat aatttctata
    39721 ggcattttca ttgatatgta ttcagtgtca acacttccgc catcatctgc aacttcttga
    39781 agtgagctat aatattttct tatcatataa atttgaccgc taccagcaac taattcttcc
    39841 atatcttcag catttaacga aatgtaattg cttcctataa tatctgtaga tacttctact
    39901 ttgttgttgt cgttatcttc aataattgct attacttctt ctttgttttc taaacttttg
    39961 ccttgccaat atattttttg gtttttttcg gcatatagtt tttcaatttt cacaatttca
    40021 atagagttta caacaacact gtttgagtaa attttgtttt ctgtgtcagt aaatttgaaa
    40081 ttgaatgtat tactatcttt tatagccaat ttataaaaac atcctctaaa agaactttgt
    40141 tcttctttca tttttatatt gttaacacta atttggctgg gttcgcttag ctttaaagtt
    40201 gtgcctttat ttccgccaaa tctaaattga gcaaaaatat catactcatt accttcagaa
    40261 gcatcgtagc taatagagta atattggtga attttgcttt gatttacatc tttagaatct
    40321 gctatttcgg ttgagccaca agcaaaacta aaacttagtg ttgaaaaaaa cactaacttc
    40381 acaatagtta tattaaaact attctttatt ccatttaagt ttattgtctt tgtcataaat
    40441 cattttttta ttttcaaata accattgaac tactttaatt attttatatt catcaaattt
    40501 aattttttca actaattcgt ttaataaaca aggttcaaaa ttaataattt ctttaatttc
    40561 ttccaaaata atatcaaatt catatttgtt aagtcctagt tcgtttcttc ttaaacatac
    40621 atcgcaatta ccacaacgtt gagtgtcagt ttctccaaaa tatgctaaaa gatgctgact
    40681 tcggcattta ttattgcttg tagcgtaatt caacatagct tccattcgtg ataatgttct
    40741 ttcttttctt tcgttaagtt ctttaatatc aaataaaatt cctttgtctt ccaatctttc
    40801 ggcattaaaa tgcattaatg gtttattaat tcttggtata tagctaatga ttttatatct
    40861 agctaaaact ttcaacaatt caataatggt atcaggtttg gtattggctt tccttgctaa
    40921 atattcttcg ctaatattta cataatcatt aaaaattcct gtataagttc taagaagcat
    40981 ttttataaaa ttatcaagtg cttcattttc tacttgaaat ttgtataagt catctctgtt
    41041 aacattaatc attattctag aaacagtatt tgcttcttct gaataagtta acatattatt
    41101 tgcttctaaa atttttatag aattataaat gcttaaaaca tctaatttaa aattttttgc
    41161 aagttcaaaa atatcaaatg ggtaatccat atctttccca tcgcctatag gtatttgcag
    41221 ataattgcaa atagcattat aaacttctat aacttttttc aaatctggaa aagctgttgt
    41281 aatatttctt tttgcttgtg ctttgtcagc agaatttgta actaaaaatg cccatgcttt
    41341 tttgccgtct cttcctgctc ttcctgcttc ttgaaaatat gcttctggcg aatcgggcaa
    41401 atccaaatga attactaatc ttacatctgg cttgtctatt cccattccaa aggcatttgt
    41461 gcaaaccata attcttatgt tgtctttttg ccattcgctt tgcttatatg ctctggtttg
    41521 tgcgtccaaa cctgcgtgat aaaaatctgc cgaaatatta tatttctgca aaagcatggc
    41581 atattctcta gttttacgtc tatttctaac gtaaatcaca gcacagcctt caatttttgt
    41641 aattaatttg aataattctt tttctttatc ttcggtatta attacgtaat agacaagatt
    41701 ttttctttca aaactttttt gtaatacgtt tttttcttta aaaagtaatt tttcttgaat
    41761 atcatcaaca acttttggag ttgcggttgc tgttagtgct aggacaggaa tatcaggaag
    41821 tttttcgcgt atttttgcaa tttctaagta tgaaggtcta aaatcatagc cccattgcga
    41881 aatacaatga gcttcgtcaa ccgcaataag gttaactttc atgtgaacaa gtttttcttg
    41941 aaaatagaaa ttttgcaaac gctcaggcga taaatataaa aatttataat cgccataaat
    42001 aacattgtct aaagctcttt ctatttcaat ttttgtcatt cctgagtaaa tagctactgc
    42061 tttaattttt cgtttttgca aattttcaac ttggtctttc attaaagcaa taagcggagt
    42121 aactaccaaa catacaccag gagttgccat agagtaagtt tggaaagtta tcgactttcc
    42181 gccaccagta ggtagcaagc caagcgtatc tttccctgaa gcaatagatt caacaatttc
    42241 ttcttgcatt tcacgaaaat tacgatagcc ccaatatttt aacaatactt cgcgaaactt
    42301 actcattaag cattaaaaat ttcaataaaa ttaattaata ttcttaaaat ttttacaaaa
    42361 ttaaaaaaaa tctttgtcat aaacacaaaa aattgtaatt tcgtaaaatt ttaattcata
    42421 atttaatgaa agaaaataat aaaattattc tcgaaggtct aacatttgac gatgttttac
    42481 tgataccagc ttattcggaa attttaccta gctccgtaag tttggtttct aatttttcac
    42541 gtaatatagt tttgaatacg cctttagttt ctgctgcaat ggatacagtt acagatagta
    42601 aaatggctat tgcaattgct cgtgaaggcg gaataggtgt tattcataaa aatatgtcta
    42661 tagtaagcca agcaaaacat gtgtatcaag tgaaacgtgc cgaaaatgca atgattttaa
    42721 atcctgtttt tatagatatt aacggaactg taggagatgc aaaagcaata atgtcagaat
    42781 ataatattgg cggtcttcct gtggttgatt ccgaaaataa actaaaagga attgttactt
    42841 ctcgagatat gcgtttccaa acagatatga gcttgtctat taaagcaata atgacaccaa
    42901 aggaaagact tattactaca gaacaaggaa tagaattgga agatgcaaaa gctattttta
    42961 tgaaacatcg tatcgagaaa cttcctgtgg ttgataaaga tgataaacta gttggtttaa
    43021 taacttataa agatattact aaagcagctt cagcaccttt gtcgtgtaaa gatagtcatg
    43081 gacgtttgag agttgcagca gctgttggaa ttacaagtga tacttttgaa agaatggaag
    43141 cacttgttga tgcaggagtt gatgcgattg ttattgatac agcacatgga catacaaaaa
    43201 gcgtgaatga ggttttaata caagcaaaga aaaaatttac aaatattgat attgttgttg
    43261 gaaatattgc aacagccgaa gcagctaagc tattagtgaa aaatggcgct gatggtgtaa
    43321 aagtgggaat aggtccaggt tctatttgta caactagagt tgtggcagga gtgggcatgc
    43381 ctcagttttc agcaattatg aatgttgcag aagcattaaa aggaagtgga gtgccactaa
    43441 ttgcagacgg aggaattaga tattcaggtg atatagtaaa agctttagct gctggtgcaa
    43501 attctattat ggcaggtgga ctttttgcag gaacagaaga agcacctggc gatacaatta
    43561 tttttaatgg tagaaaattc aaagtttata gaggaatggg ctctgtagaa gctatgaatc
    43621 aaggctcaaa agatagatat ttccaagata tggaagaaga tattaaaaag cttgtgccag
    43681 aaggaatatc tggaagagtg ccatttaaag gtaatttgca cgaaattgta tatcaactaa
    43741 tgggcggatt aaaagctgga atgggatatt gcggtgcaaa gaatattcaa acattacatg
    43801 aaaatgctaa atttgttaga ataacttcga gtggtattgt ggaaaaccac ccacacgatg
    43861 ttacaatcac aagagaagca ccaaactact caagaagtga ataattatga atttttattt
    43921 gatttaaaat aatatagtat gaaaaagatt tttgttttat taagtttttt agtttttgta
    43981 aacttagctt tctctcaatc taatgtaatc ttagaaattg atgatgaaaa aattactaca
    44041 gatgagtttt tgcatattta taagaaaaat aacacaaaca caaatgcaat gacttatcaa
    44101 gctatggaag aatatttaga tttatttgtt aattttaaac ttaaagtaca cgaagccaaa
    44161 cttttaggtt tagatacttt aaaatcgttt aaaaatgaac tttcaggcta tcgtagtcaa
    44221 ttagcacaac catatttaac agataaaaaa gttgaagaag atattttggc agaatcttat
    44281 gaaagaatga gatgggatgt tgaagtttcg catattttaa taaaatgcga ccaaagtgct
    44341 agcattgcag atactgtgaa agcttggaat aaaattaacg atatttataa aaaacttaaa
    44401 aaaggagaaa gttttgaaga acttgtgaaa aaatattccg aagacgaagg aactgtacca
    44461 acaaatggta gcttgggtta taaaacagtt tttaatttag tttatgaatt tgaatctgct
    44521 atgtatgaaa ccaaagtggg agatttttca caaccattcc gctcacgttt tggctatcat
    44581 attttaaaag ttacagataa aagaccagca aaaggaaaat atcaaattgc tcatgtaatg
    44641 actatagtgc cagaaggttc tagcaggcac ttatggaaaa aagcagaaga taaaatta
//
LOCUS       DAOPNX010000010        42966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_1159_length_42966_cov_5.949733, whole genome shotgun sequence.
ACCESSION   DAOPNX010000010 DAOPNX010000000
VERSION     DAOPNX010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 42966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42966
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1159_length_42966_cov_5.949733"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PKN53_02050"
     CDS             complement(<1..464)
                     /locus_tag="PKN53_02050"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOD87768.1"
                     /translation="MKRKFAQNLLDVMQYSKEEAIRLGNDYLGPEHLFLGILREPNCD
                     AMEILKSLDINTLELKFKIENHVKNVNNQFTMDDMDKIVPTKTTERIQKFMYSEAISL
                     NSEEVHTGHLLLAILKVPSRFSQLLDELNIEYDMIKMTMELDDFSDVSSSRE"
     gene            731..1978
                     /gene="hutI"
                     /locus_tag="PKN53_02055"
     CDS             731..1978
                     /gene="hutI"
                     /locus_tag="PKN53_02055"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOD87769.1"
                     /translation="MKILIKNIKELVGVDNKARLLVKGADMAKLNTIKNAYLLINGEI
                     IEDYGKMEDLSNSLLDNGKIKEIDASGKMVFPSFCDSHTHLVYAGSREIEYSDKIRGL
                     SYEEIAKRGGGILNSAKLLHKTSEDELYRQAMQRVNEIISQGTGAVEIKSGYGLNTED
                     ELKMLRVIKRIKETTPLIVKSTFLGAHAFPTEYKDNPDEYVDLIINEMIPQVAAEGLA
                     DYVDVFCDKGFFTVEQTDRILMAGIKYGMRPKIHANELAKSGGIQVGVKYNALSVDHI
                     EYTEEEEIQALLNSETMPTILPGAAFFLNMPYSPARKMMQAGLPVAIASDYNPGSSPS
                     GNMKLMMSFACVNYKMLPEEAINATTINTAYAMGVSDEVGSISKGKIANVFITKEIPT
                     YEFFPYAYGSDLIDTVILKGKIR"
     gene            2055..2225
                     /locus_tag="PKN53_02060"
     CDS             2055..2225
                     /locus_tag="PKN53_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HOD87770.1"
                     /translation="MENRDLILKVLKGKEMKAGEIAEQTGLDKKVVDKEMNALKKENL
                     IISPKRCYWTAK"
     gene            complement(2375..3457)
                     /locus_tag="PKN53_02065"
     CDS             complement(2375..3457)
                     /locus_tag="PKN53_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOD87771.1"
                     /translation="MILPESLSIDWLQRIARENNFPNITMLEKAVRALSLLESLALSG
                     CPFIFKGGTALMLHLDSSKRLSIDIDIICPPETDIEKYISKNIEKYGFREIKLIERKT
                     TNLIPKSHVKFFYEVTYRTNIKKDYILLDVMFDDNYYTNLVSLPIESKFLKTEGKQIF
                     VLVPSVEDMLGDKLTAFAPNTTGIPYYKNNRLCSMEIIKQLYDIASLFDIVNDLTVTT
                     NTFHRFAKVELAYRNLDMTNIQQVLDDIFQTSLCICLKGQEDKEHFLLLQDGIKRIQS
                     FIHSERYVLDTAVLQASKVAYLSTLIANKLTKIERFDRSVISDLKNASIENPMSTKLN
                     KLKKTNIEAFYYWFKTNEINNQIIAE"
     gene            complement(3454..4146)
                     /locus_tag="PKN53_02070"
     CDS             complement(3454..4146)
                     /locus_tag="PKN53_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOD87772.1"
                     /translation="MSISTSILNFAAGRLSFSRSELLEYLSDLSDNSLSQQLYRLVKA
                     NRLERIGHGIYRLPASYLPISNEMEEINSKLKEQFPFVHFCLWSSDFIMPFMHHIPEL
                     NYIYIDVEREATEAIFYFLKNELHRPVFFRPSKEEYQRYIVGNQAVIIRALISESPLL
                     SLNNMQVPTLEKILVDIVDDIEFDFLQGTEILYFYRNVLDSKKINKRKLFRYATRRGR
                     LNQVKKLYNDAL"
     gene            complement(4240..5469)
                     /locus_tag="PKN53_02075"
     CDS             complement(4240..5469)
                     /locus_tag="PKN53_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD87773.1"
                     /translation="MKKIIYLFALLSLTLNLNAQSGFSFSFSSALESDNQHFTGLYAF
                     ENKYFATTDYNNASYYYDGETLYLCPDFDINLMSGVEKSNEYLFVACIKYRYSGETSG
                     YYYIYTWHSDSINWEKSFENQNQEFTKIFVLNENEIYVWGHDSNKKKYIWLFNPNEKT
                     LTEMYNFHDINTYSCEFMEAKSSNNILLLLKRGQKGLWQRYNGVNLEVLFDFGKINSF
                     DEIKASHDKSIFFFLPDSFDKLYLWKEEDQKMDSIIFDMSVQSFFPLDNENLMLFSGA
                     ENYCFNIKSGEKERVYGGPGPVNMSDYAGKTTAFVASGTKLYFMFITGSNVEFKEINN
                     ISLYPNPANNVLRLECPVEGAKHVKILDLRGQILMEESFYDSDNMSLNIANLSSGVYV
                     IQINTTKGVVNKKFVKN"
     gene            complement(5515..6612)
                     /gene="lpxB"
                     /locus_tag="PKN53_02080"
     CDS             complement(5515..6612)
                     /gene="lpxB"
                     /locus_tag="PKN53_02080"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOD87774.1"
                     /translation="MKYFIIAGEASGDLHGSHLIKELKLLKPNAEIQAWGGNLMQEQG
                     AFIKKHINELAFMGFVEVLENLRTILKNFKLCKKQIKEFNPDTVIFIDYPGFNLRMAK
                     WVKKNNYKSVYFISPNVWAWKSSRAYKIRDYIDQMIVILPFEKEFYKKYGVEVKYFGH
                     PLMDAIKNHEKTSFEEFCAENNLSNKPKIAVLPGSRKQEIKHILPIMTEIVDKFPEYE
                     FVIAGTSTIPIDLYKQNIKSENVKLLFGKTYDILANSTAGLIKSGTSTLEAALFDLPQ
                     VVCYKGGKISVAIAKIVAKVKYISLVNLILDKPSVKELIQNDLNKDNLIQELYKILPK
                     GENYQTIKADYNVLFKLLDSKDIYKNIARIL"
     gene            complement(6612..6923)
                     /locus_tag="PKN53_02085"
     CDS             complement(6612..6923)
                     /locus_tag="PKN53_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87775.1"
                     /translation="MNTNKTKKLNKTWFGFALGMILPIISFLVYYMYKLSINNEMSLK
                     KFIDILIENEALIAVLSICLLPNLLFFFLLQKLNYWYAIKGVIATVVIYTLFVVVIKL
                     M"
     gene            complement(6933..7706)
                     /gene="surE"
                     /locus_tag="PKN53_02090"
     CDS             complement(6933..7706)
                     /gene="surE"
                     /locus_tag="PKN53_02090"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOD87776.1"
                     /translation="MKDRKLILVSNDDGVDAKGLKHLVEIAKKFGDVFVAAPEREQSG
                     KSHSINLGEPLRVRRIEREDGVKFFAIKGTPVDCVKLAINQLVPRKPDLMLSGINHGS
                     NSAVNVIYSGTMGAAIEASLYGIPAIGFSLLDHSKDANFDLIDENADVIIKDVLENGL
                     PYQVSLNINYPVIDKKDFKGYKICTQTKGVWKEEYEKRHDPYGREYYWLTGEFTNHQL
                     DNPETDEWALKNNYAAIVPTKIDFTEYNTIEILKSRLKV"
     gene            complement(7770..12236)
                     /locus_tag="PKN53_02095"
     CDS             complement(7770..12236)
                     /locus_tag="PKN53_02095"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOD87777.1"
                     /translation="MNRIRIKRFLLILSLLIVKYLAIVIILINSAFLQSKLTKFVIDY
                     FAKHYDIALSIDNVYIKLPKTIVLQNITLLDEQNDTLLCSQEINAKIASISLRNMDLN
                     LEKISLINPYISVQVDSLGVANYESILEKFASDPDTTSDSEETDFSLSCNNFEIVNAR
                     LKYKDVRYLNNIEDEFNYSDAEISGFNLELSDFKYGADTIQLRLNNFSFAEKSGLNIE
                     KIAGHLKYYDTGIELNDFVFKTKLSEFICSEIAISGDSTDYLSDPIHKAKLKINIDTL
                     IFNIADLAPIKPEYRFMRDKIYLSGNFNGNLSKLKLTDFNLNYGENTRFSTDIDIDGL
                     DNPEMAFIFGNVSELRINKRDIDRLLRIIDPENPMILPSIVSDIDNLSFNGNITGFLS
                     DLVAYGEFRTPLGSIRTDIALVTDFESFDLDYSGKINLMSVDAGKLLGDSETFGKISM
                     NAIIAGNLDSLGNYNANIKSKVSELDILKYKYSEITINGDISNTLFNGEIFVDDPNFK
                     FQFLGQYALGRNTERVNFSTDLYANLNALHITNDSLESEIKFVMSADVEGPLSTKPLG
                     NLNINDLKLVLNGQAVKLEHFNLESFIDEKQMHCLNLKSDYIDLKFAGDFEFDELGVR
                     VYNLLSHYAPSYIKPIEVKDDTKSNTAKFDLRLKNLSEVLKLFMPDLVIDDDVFAEGS
                     INGKEEKFDMVFSFPVIKYDSIYLWGSELNVVGNSKLLNIGLTSQEISSKSFPWLENI
                     VLNLGVQQDSVLLELNWNNFDDINNSGNINILTKFIPVKDRAMPKIESSIFESNLVIA
                     NRNWEVRPSSVIVDDKNITINKFGLVHENQMLNIDGEISENPTKLLRFLISDVDITNF
                     NPYLAFTGYQVQGALSGNGRVANIYDNPSLKASVSINDFKINNEDFGRFDISAIWEGR
                     KNGFKIDGVNKFMKIRGVYMAETDSLDVNFLMDNFNLEILEPYTSDFDISNLKGHLDV
                     VVDVRGKLAQPDISGYIEFQKAELVYDFLKLHAFINDKIFITNDAISFKDFKIYDEHN
                     NKGVINGGLYHKNFSDIRFDILVDATNMKLMNTTEKDNSLYYGTAFATGNVRIAGDLD
                     KFGIDVVAKTEPNTVFVLPMTESYEGSDVSYVTYIQRPKEDEEGETETKAIKTDMEYY
                     FKLDVEVTPDAEAQIVFDSKVGDLIKGYGSGNLKMEYTSDEEFYMYGDIEIVSGDYLF
                     TLENIINKKFHIKPGGTIVWSGDAYDAQLDLDAVYHAKAPLIDLMAAVADSSNIYKKP
                     TNVECHMHMSGSLMSPDIKFSIEIPNANDKVKTQLANMTQDEINKQMLYLLILNRFYS
                     ADAENVSPQIGNTTNAFGVTSAELLSNQLSNWLSQMSKDFDIGINYRPGSEVSERELE
                     VALSTTFLNDRLLINGNVGVGENKSTTSNLIGDIEVQLKMNQQGSFRLKGFTRVNPET
                     QAELGPYTSGVGVFYTEDFDTFGELVKSLWDKITFKKAREKRKAKKEKQVIY"
     gene            12351..13376
                     /gene="tsaD"
                     /locus_tag="PKN53_02100"
     CDS             12351..13376
                     /gene="tsaD"
                     /locus_tag="PKN53_02100"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOD87778.1"
                     /translation="MKTIVLGIESSCDDTSAAVVCDGKLYSNVINTQSIHYKYGGVVP
                     ELASRAHQQNIVLVVKEALEQAKISFDDISAIAFTQGPGLLGSLLVGSSFAKGVAIAK
                     DIPIIGVNHLQGHMFAHFLREKDEEEFIPKFPFLCLLVSGGHTQIIVVESYTKYEIIG
                     STIDDAAGEAFDKCAKVMNLGYPGGPLIDKYAQTGNPNKFKFSKPNVADLNFSFSGLK
                     TSFLYFLRDQIKENENFISENLPDLCASLQFQIVDILLEKLSKAVKQTGITEITISGG
                     VSANSGLRIKLTELAQEKDWTVHIPKIRYTTDNAAMIALIGYYHFLDGKTDGLDIVSV
                     ANLAQLS"
     gene            13523..14086
                     /locus_tag="PKN53_02105"
     CDS             13523..14086
                     /locus_tag="PKN53_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOD87779.1"
                     /translation="MNKEFANPWHSVSPGKDAPEIVNGIIEIPKGNRAKYELDKNSGL
                     LKLDRVLYSSVYYPVNYGFIPQTYCNDKDPLDILIMAQIEIVPMCIVPAKVIGVMRML
                     DSNVADDKIIAVSAGDPSMNHINDISELPDHFISEIRNFFEEYKKLENKTVVVEEYLD
                     KYTAMEIINESFEEYKQKLEFLKSYKF"
     gene            14098..16524
                     /gene="pheT"
                     /locus_tag="PKN53_02110"
     CDS             14098..16524
                     /gene="pheT"
                     /locus_tag="PKN53_02110"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOD87780.1"
                     /translation="MNISLNWLKNYINISDEPETIAADLTSLGLEVGTITEFSSVKGG
                     LKGLVVGEVLTCEKHPNADKLSLTTVDIGSGEIQKIVCGAPNVAAGQKVIVATVGTTL
                     YSNDESFTIKKAKIRGEESNGMICAEDEIGIGSSHAGIMVLPNDTPVGLPASKYFNIE
                     EDVVFEVDLTPNRIDAASHIGVARDLAAFRNIKYNIPDVSKFKVSKNTYSIDLEVANP
                     EACPRYSGICMDNVKVTDSPQWLQNRLKAIGLNPINNIVDVTNFVMFETGQPLHAFDG
                     DKIANNKIVVRTVDENHKFVTLDGVERSLKSTDLMICNAVEPMCIAGVFGGLDSGISK
                     NTKKLFIESAYFNPGWVRKTAKTHGLSTDSSFRYERGADVNITLYAAKRAAMLIEQIG
                     AGEISSELKDVYPHKIENNIVDFSYETCCKLIGKEISKPEIDKILTALEIKILESKDD
                     NLKLEIPSYRVDVTREADVIEEVLRIYGYNNIENPEKVSFSVLNSEKLSLDKLQNTIS
                     DLLVSDGFNEIKCNSLISAKYFNQDDKSIVRICNPLSADLAIMRPTPIIGGLETIVFN
                     VHHKISDLKLFEFGRTYHANTEIAELSKIERYYESKFLALWITGNKDVLSWNQKEKPS
                     DFFTLKSYVTKITTRLGIDLAELKEDFVSDSNFEYALQYKYKEIVFCSFGKLNAKLAK
                     STDCDKDVFYAEINWDLVKELVAKNKISYKSISRFPTVKRDLALVIDKNISYKQMVDL
                     AKKTEPNYISEVNLFDVYEGKNIEQGKISYALSFSLDDHEKTMTDKQINKIMERLIKA
                     FETQLNAILR"
     gene            complement(16608..17636)
                     /locus_tag="PKN53_02115"
     CDS             complement(16608..17636)
                     /locus_tag="PKN53_02115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD87781.1"
                     /translation="MMKYLITNKLGQIIVSNSDVQEIETEIIHCNSDCERILCKYTNE
                     FRRQGVHKTESYNYYLYSYDKKLTNRIFKFYLDAFSILAIHLEKDLIVQKSKESQKTR
                     RLKHNLITHSSNILLELYKLFPQDYFRNGNNHLDNIKNIIEKDISKAAYTYLKILKNS
                     NLMKAEFDVYEMLENENPYMDFSKHPIHKVLLLTLNPFWLDLVERNIVIDIDQYHGTV
                     TIDYKSVSVILSHIFDNMSKYIMPHSTLHILFKEEDEKVQIQMEMTSLKVEPTELKNI
                     FTENYSGHWAKKLDLGGDGIGMYVIQKLTRLNNGNVYFEINVDNKDTIFLNSKPYENN
                     RIIIELKK"
     gene            complement(17633..18277)
                     /locus_tag="PKN53_02120"
     CDS             complement(17633..18277)
                     /locus_tag="PKN53_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87782.1"
                     /translation="MIFWKKRKSVLKERNLNEIKSVCKILFIDDKVFPVVDILKNAGW
                     QNTQRIKDAESIDQTEIREAHILFVDIQGVGKKLKFKDEGLGLIIALKEKYPNKKVVV
                     YSAEDQGKIQAFHAGIDKADKRLSKNADPYQFQTVVENYAKEAFSLKECIERIKQQIL
                     IEFGYTLESEKIQKYLNTIYLKKDFSAKNIANVFNLQNGASLANIIQLFFTASL"
     gene            complement(18554..19963)
                     /locus_tag="PKN53_02125"
     CDS             complement(18554..19963)
                     /locus_tag="PKN53_02125"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOD87783.1"
                     /translation="MEGLEVEEVELSETFKNKDFRIDSDFWTKEPYRNPKLKYDKIGN
                     VLIKAQYGISISMNEDNVGYPIYRMNEIHNMLTDLDVEKSADISKEEFIKFKLKDRDV
                     LFNRTNSYEWVGRTGLYRKVEKQEYTFASYLVRFVPDENIVNPEYLTAFLNTKYGIRD
                     IKRRSRQSINQTNVNPEEVKEILLPILNKSIQLEIKNCFDTAHESRLKSKQLYQQAET
                     LLLETLGLTDFELSSDPINIKSFKESFLETGRLDAEYYQKKYDDYLELIQNYSSGFDQ
                     LASVCELKDKNYNPKDNIEYKYIELANIGTSGDITDCTVSRGVDLPSRARRLVKADDV
                     IISSIEGSLSSCALVTEKYNKALCSTGFYVINSKQINSETLLVLFKSEPMQNILKQNC
                     SGTILTAINKNEFQNIPIPLIKIEIQKQIKEKITESFRLKQQSEELLEAAKLAVEIAI
                     EKGENEAITHLKNQLKVKS"
     gene            complement(19999..22077)
                     /locus_tag="PKN53_02130"
     CDS             complement(19999..22077)
                     /locus_tag="PKN53_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOD87784.1"
                     /translation="MTLADILRDTNYRLTQFSDDKIKELEENIIIRESRNKIEPYINC
                     IVRKKEIKLTPEEVIRQLYVMVLTQDLAYPISRIELEYAVTFGREKKRADIVIFDKIQ
                     KTTPYIIVELKKPKLKEGKEQLKSYCNATGAPIGIWSNGESISYYHRKDPNYFEDIPN
                     IPRADQKLSDILEERWTIENLIEKDKLVTERKSLKDLILEMEDEVLANAGVDVFEELF
                     KLIFTKLFDEMESGRNPSRHLEFRNYGDTESELKNKLQGLFDNAKQKWEGVFTDDAKI
                     MLTPSHLSICVSSLQDVKLFNSNLDVVDEAFEYLINKSSKGEKGQYFTPRYVIDMAVK
                     MLNPKEDETMIDTASGSCGFPVHTIFHVWEQIMKDEGLNKNHLFTIDAKPPRCIDYVQ
                     DKVFAIDFDEKAVRVGRTLNLIAGDGQTNVLHLNTLDWERWDEKTEDEDWQDIYYLGW
                     KKLKKLRADKNSNRDFTFDILMANPPFAGDIKESRILAKYELGKKEDGKYQTKVGRDI
                     LFIERNLDFLKPGGRMAIVLPQGRFNNSSDKRIREFIAERCRILAIVGLHGNVFKPHT
                     GTKTSVLFVQKWDDKLCPKLDDYPIFFATMQEPSKDNSGDKIFVRKKDFPKAAEGNFH
                     SAEIEDSDILSDVQNHYQATPNDLEEYLLDSHGHLIVKHDLFNHDNCTKDGIAEAFIE
                     FAKKERLSFF"
     gene            complement(22173..22736)
                     /locus_tag="PKN53_02135"
     CDS             complement(22173..22736)
                     /locus_tag="PKN53_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87785.1"
                     /translation="MKTSTLIFLLLAMFFINSCRNIECPCFDKSQLTWMPYEVGDRLI
                     YVNQQNDSLIFTVKSKHLSEAYKEKKRFEDDCISNAVFHLSDTERSQYYFNIDNNEAG
                     TSIACKISLQISDNVFSSGIASSYIEGKLDTMTINGKFYNEVLVIERDTATYSENIWK
                     IIIANNYGIIRFFDRKTGYVWTLSVKS"
     gene            complement(22866..23891)
                     /locus_tag="PKN53_02140"
     CDS             complement(22866..23891)
                     /locus_tag="PKN53_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87786.1"
                     /translation="MKRIYLIIISLISLFIFSCSTDVDVNGEWDDIPIVYCVLDQSTE
                     YQYVKVNKSFLGNKPASEMAQHSDSLFYKKVDVYIHEYVNTYKTRTWTFEPVEIDKEE
                     GYFANDKNIVWRQKMDMRDDAVYKLEVNINDGEHIVTGETKLISGISITMPSGVEVLP
                     VELRHYAGNSEYRYYNGENGKVFQMCLTFNYFEVYNDDTTYYSIPWLQAKTYKTTEGN
                     SEVSGKFSVVAFYNLLQSNIPAPREGAKRYVKMPESLVYNLVVGDENYMIYMDITEPS
                     SGLAQDKPAFTNLNDGFGLMASRYNVYRAKKLDRYTLDSIHRGIYTKHLGFVSPYDDY
                     YKPYFIY"
     gene            23960..24772
                     /gene="panB"
                     /locus_tag="PKN53_02145"
     CDS             23960..24772
                     /gene="panB"
                     /locus_tag="PKN53_02145"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOD87787.1"
                     /translation="MSVHKEIKKITTNVLLEMKRNNEKISMLTAYDYSLAKIVDAAGI
                     DVVLVGDSAANVMAGFDSTLPITLDQMIYHAESVVRAVNRALVVVDLPFGTYQGNSEL
                     ALSSAIRIMKETGAGAVKLEGGLEIADSVKRIISAGIPIMGHLGLMPQSIHKYGTYTV
                     RAKEEQEAKKLIEDAKFLEESGCFSIVLEKIPAKLTQQVLKEVDIPIIGIGAGPADGQ
                     VLVLHDMLGITQEFSPRFLRRYASLDKIMLDAVNQYIEDVKSTDFPNENEIY"
     gene            24778..25461
                     /locus_tag="PKN53_02150"
     CDS             24778..25461
                     /locus_tag="PKN53_02150"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOD87788.1"
                     /translation="MHKQLEIIYEDNHIIAVNKRASQIVQGDKTGDRALSELVKEFLK
                     EKYSKQGNVYLGLPHRLDRPVSGVVLFAKTSKALTRLNAIFKSASVQKTYYAIVANPP
                     AKTEDKLIDYIYRNTKQNKSYVVDSSNQEAKKAILEYKLIAKSERYYLLEINLITGRH
                     HQIRAQLANMGCPIRGDLKYGYPRSNKNGGINLHSRKISFIHPVQKEEITITAPFPEE
                     KIWTIFNIS"
     gene            complement(26025..27932)
                     /gene="rpsA"
                     /locus_tag="PKN53_02155"
     CDS             complement(26025..27932)
                     /gene="rpsA"
                     /locus_tag="PKN53_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOD87789.1"
                     /translation="MTEINDNVNVEPQKTEEVQNHEIKNEANTETVVAEEKAKPEIKP
                     LENFNWDLIGEKDDAYSKEERAKLEELYNNTFKLIEEGEVVMGTVVAINKREVVVNIG
                     YKSEGIINANEFRTETEIKVGDKVEVFVENMESIDGQLILSHKKAKSLKSWDRVNEAH
                     DNDEIVKGYIKCKTKGGMIVDVFGLEAFLPGSQIDVKPIRDYDVYVGKTMEFKVVKIN
                     EEFKNVVVSHKALIEAELEEQKKEIVAKLEKGQVLEGIVKNITSYGVFIDLGGVDGLI
                     HITDLSWGRVTHPEEIVSLDSKIQVVILDFDEDKKRIALGLKQLTPHPWDALDANLKV
                     GDKVSGKVVVLADYGAFIEIAPGVEGLIHVSEMSWSQHLRSAQEFMKVGDDIEAVILT
                     LDREERKMSLGIKQLHPDPWENIETKFNIGTKQTAKVRNFTNFGIFVELEEGVDGLVH
                     ISDLSWTKKIKHPAEFTTIGAELEVVVLEIDKENRRLSLGHKQIEENPWEVFETIFTI
                     DSIHEGTIVEMSDKGSIIALPYGVEGFSTPKHLIKEDGSSAKIEEKLDFKVIEFNKDS
                     KKIFVSHSRIYEDLNKEKEAESKKAKGEKTTKRTKAQIENKIEKTTLGDVTDLAAIKE
                     ELLEKEKKTKK"
     gene            complement(28140..28213)
                     /locus_tag="PKN53_02160"
     tRNA            complement(28140..28213)
                     /locus_tag="PKN53_02160"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28177..28179),aa:Arg,seq:acg)
     gene            28409..29719
                     /locus_tag="PKN53_02165"
     CDS             28409..29719
                     /locus_tag="PKN53_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOD87790.1"
                     /translation="MKTPIDVKLVDAQIEKLGVKDLSKATIREIVTLVNWIHEESGKE
                     FVRMEIGEPGLTPPSVGIEAEINALKSGVASKYPMISGVPELKNEASRFIKNFINIDI
                     SPNSCIPTVGSMQGGYATFLICANLDAKKDTALFIDPGFPVQKQQYRVMGHKFESFDI
                     FEYRGKKLREKLESYLSKGNINSIIYSNPNNPSWICLTEEELQIIGELATKYDVIVIE
                     DLAYIAMDFRRDLHQPGKPPYQVSVANYTDNYVLLISGSKAFSYAGQRIAVAAISDKI
                     YNRKYPNLLQRFGTEGFGYTFVSRIIYALSSGVSHSAQYALAAMFKAANDGKFDFVKE
                     VKEYGVRAKKMKEMFLDAGFKIVYNKDLDEDIADGFYFTISYPGMEGGELVKELLYYG
                     ISAISLLTSGSTRSEGLRACVSQIVPDKMKLLEERLKVWKENHK"
     gene            29725..30063
                     /locus_tag="PKN53_02170"
     CDS             29725..30063
                     /locus_tag="PKN53_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87791.1"
                     /translation="MATHVLREDKDINSVSGLLQEKYPQAKFKNRKTNIIIKNKMIML
                     VIRQGRKSFKITGGLNFSYLPLLILIIASILLSTAALILVLIILEIIFRRKINKIKKD
                     VANVLFGAVG"
     gene            30029..30817
                     /locus_tag="PKN53_02175"
     CDS             30029..30817
                     /locus_tag="PKN53_02175"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOD87792.1"
                     /translation="MLQMFCLEQLVDFQNVYRDLLKHYIKGFKDACKDENVLLNIELK
                     EVHSLNVAENCLKIAKSENLNQEKTLIAYLCGLFHDVGRFEQFTKYHTFRDNESLYHG
                     ELGVEVIKKEQFFKDLPKETQDIICTAVYNHGLREIQANVKNDELYFSKLVRDADKID
                     IYRIVCKYYNNSGPRNIALEYGLEDKAEISTKPLNDFINKNLISKEDLESVNDFKLMQ
                     ISWIYDLNFNFSKQIIKEKAYIDTILDTIKGENVKQIIKQYITL"
     gene            30875..32239
                     /locus_tag="PKN53_02180"
     CDS             30875..32239
                     /locus_tag="PKN53_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOD87793.1"
                     /translation="MKAFKAYDIRGIYNKDFNKEDVYKIGFFIPQVLLTDKVLVGRDV
                     RESSPEALEYLVKGILDSGADVYYAGLATTPMIYYSTAKYGFGASVMITASHNPAEYN
                     GLKVSGKNAKPIGYDTGLKKVEELMKTQEIIVSDQKGKYHEINVRDTYFDFLKSYVPK
                     LNDLKISMDISNGMAALFIKELLGDKVMYFYDELDGSFPNHEPNPLVPKNIVDLQKHV
                     IENKSDIGVIFDGDADRVMFVDEKGTFISPDLMIAVLAHYFLEEKGLKGKVIQDIRTS
                     KAVGEYIAQFGSEMEMWRVGRAFAAPKLKEIDGIFGGELAGHYYFKDFYYSDSGILAC
                     LLILRVVAQMHEKGITVSELIGKIKKYENSGEINFKIEEKTKAMDAVIAHFKEKEKPD
                     AFFDFDGYRIEYKNWWLNIRPSNTEPYLRFLAEANTEKLLKEIVDTVVGIVESYGGKL
                     VSGH"
     gene            32333..33391
                     /locus_tag="PKN53_02185"
     CDS             32333..33391
                     /locus_tag="PKN53_02185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOD87794.1"
                     /translation="MKNFTEETTNFSFNRLPKDLQKSLSTIYSHTEPVANPLKVNAIH
                     QGDASELLKKIEPNSIAVSVWSPPYFVGKNYEKHLSFEGWKNLLKSVIANHFSIIKPG
                     GFLAINIADILVFKDEKMPKIQADAVHRKRISISKENILEVLAKNPTMNRNDLAKHFK
                     CSEQTIDRRLHGNNVRGGKQETQSRVKIIGGLVENWALEAGFYPYDRRIWVKDAAWEN
                     SRWANLSYRSVDEFEYIYIFWKPGITRYDRNRLSNDEWKNWGSRGVWQFPSVRANDDH
                     EAKFPLELPTRIIKLLSDENDIVLDCFMGSGTTAIAAIKENRNYIGIELNPEYVKLSK
                     ANILKETGVNSIITFNAN"
     gene            33381..34085
                     /locus_tag="PKN53_02190"
     CDS             33381..34085
                     /locus_tag="PKN53_02190"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF022924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SfiI family type II restriction endonuclease"
                     /protein_id="HOD87795.1"
                     /translation="MLINPNTLQDNLERLEAIEKASLRLVVQAIYDFRESAKEIFEFE
                     SDLVADIGEDITREALDKLGVSKIEQRLFGKIDYKKARYLFQPDFAIKQALFVDSKAE
                     KISGRGTATLQTSQFSLIVKQFRANEHIEVKGKLPCIIDNSFITTTIFVKYNYEEIKG
                     KNILNDITIAALPNGFLQNIYNPTAQDTIFVAGRNAPTRDEEFRVRLSFSRLKAKAKW
                     RVQNIQMSPNEFHWDE"
     gene            complement(34150..35781)
                     /locus_tag="PKN53_02195"
     CDS             complement(34150..35781)
                     /locus_tag="PKN53_02195"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOD87796.1"
                     /translation="MEFASSEYSKNVGIKIAKATKLANVSTATIRNWIKTGYLTKINA
                     GLISKKSFDKFMQEVAGKEKLNSRSNKLLKDSHDHVKVRKTLKNLIDTTKSADIGSEY
                     EKLLSNSYKNKEGIYYTPPCIVRDMFKNIYISPDFKFLDPCCGSGNFIIEAIRLGVKP
                     ENVYGYDTDENAVMITRERIKKEFRYESPNIICCNFLEKAPKLKKSNILFDLIFTNPP
                     WGKKIDKQDKQKYSKLFNCGNSFDTTSLFLAASLNILKNKGKLGFLIQEAFFNIGQFE
                     DIRRKIIKKKILRFVDYGKAFNSLITKAKAIIIENKTSSKNDEIICENNDKSHTRFIS
                     SFINNPKCIFNFEATNEENKIINHLYSINHISLKSNADWALGIVTGNNKKFCISQEKE
                     GYLPIYKGSDITKSGLKKPSNFILNDFSQFQQVAPIENFLAKEKIIYKFITSNLCFYC
                     DKNQVLILNSSNILIPKNIDISAQQLTDLLNSEIINWLFKKIFSTHKVLRGDLELLPI
                     HNGYFRKYEKFTEENYLKYLQIQKDNNGEYQLVKS"
     gene            complement(35799..37199)
                     /gene="gltA"
                     /locus_tag="PKN53_02200"
     CDS             complement(35799..37199)
                     /gene="gltA"
                     /locus_tag="PKN53_02200"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOD87797.1"
                     /translation="MMAKIPRVPVREQAAQERIKNFEEVSYGYNAEEAVLEAHRCIQC
                     KNPRCVQACPVQIKIPEFISAVKNKDFAQAADIIAEDSSLPAICGRVCPQESQCEGSC
                     VLGVKGEPVNIGKLERFVADWGRKENYYNPTKVPTNGFKVAVIGSGPAGLACATDLAK
                     LGYQVKIFEALHLPGGVLEYGIPEFRLPKEEVVRYEINNVIKLGVEIETNVIVGRTIT
                     IDDLLDKEGYHAIFLGSGAGLPKFMNIPGENLNGVVSANEFLTRSNLMKAFDSEYDTP
                     IYVGKRVAVVGGGNVAMDAARVARRLGAEVYLVYRRSEKELPARVEEVHHAKEEGIIF
                     KLLNNPVEILGNEKGWVTGMRCVEMGLGEPDESGRRRPIPIKGSEFDIDCDVVIMSLG
                     TSPNPLIASTTPNLNTEKWGGIIIDENTSQTSREGVFAGGDNVTGAATVILAMGAGRK
                     AAEQIDVFIKNKFGKK"
     gene            complement(37201..38070)
                     /locus_tag="PKN53_02205"
     CDS             complement(37201..38070)
                     /locus_tag="PKN53_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOD87798.1"
                     /translation="MYKIVKKRPLAEQIFLMDIEAPRVAKSAQPGQFVILRVRDKGER
                     IPLTICDYDREKGTVTIVTQCIGVSTHLICEHNEGDYILDFAGPLGQASELIHATDEE
                     LANKKILFVGGGVGAAPVYPQVKYLSNRGFKPDVIIGAKSKNYVILEEEMRAVSNNYY
                     ITTDDGSYGFKGMVTDKLKELVEKENKKYDLVVTIGPMIMMKFVSLLTKEYGIPTVAS
                     LNTLMVDGTGMCGACRVSIGGQTKFTCVDGPEFDAHLVDFDEAMRRQNMYRTIEGQKR
                     EELEKNHKCKIGR"
     gene            complement(38229..39119)
                     /locus_tag="PKN53_02210"
     CDS             complement(38229..39119)
                     /locus_tag="PKN53_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOD87799.1"
                     /translation="MWKLIVKIILGLVFIASAILKLFTIDDFELYIFTINLFSLDLSI
                     LLARLIIGFELFLGVLLCLNLYKFITDLTLITTAAFTVYLGIRAIGGDVENCQCFGSN
                     IFLSPVQSIIKNAVIIAGLVWVRNSKYFKFKYKKLVTISIFVISIVGVNIISPPDFIY
                     YKQYSKLTDIYSVEHFEKFKENPVLKERQNKKTIICFLSPYCKLCNLTAKKISSMVKN
                     HNIDKSNVLYIFVESDDIAKFLDDTKSTDIKYIQMPKEEVMQITAGGVPVIILYENKV
                     VDAFVYRGIVERKIVDFFNN"
     gene            complement(39338..39535)
                     /locus_tag="PKN53_02215"
     CDS             complement(39338..39535)
                     /locus_tag="PKN53_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87800.1"
                     /translation="MKKTIFIALALVGIALLGFTSCSKECTCTTYINGKEISSVTFEK
                     EKGTKCSDGNQKVLNTEIKCK"
     gene            complement(39753..41861)
                     /gene="recG"
                     /locus_tag="PKN53_02220"
     CDS             complement(39753..41861)
                     /gene="recG"
                     /locus_tag="PKN53_02220"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006281290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOD87801.1"
                     /translation="MAILDNELTYLPGVGPKRAELLKNELGLTTWNDLLYYFPYRYID
                     RSKFYNINELNKDLPYIQIKGKFTHLETSNISRNKKKLTGYFTDATGVIEIVWFNATN
                     WIISSISRDKEYVLFGKPTLFGNRLNIVHPDIEEFTKWQKQLVSNLYPQYSTTEKLKT
                     GYINSKVIQKLCDTLLKQVKGQIPETLPPWFRQKYNLKGLEETLFELHFPTDTQSLAK
                     AQYRIKFEELFYIQLNLMQLKLNRKQNTPGFIFDRKKDKILKDCFFKNIKFKLTNAQT
                     RVLREIRKDVASGKQMNRLLQGDVGSGKTLVALLAMLMAVDNGYQACIMAPTEILAQQ
                     HLKSISNFLKDLDVNVALLKGSVKKKDRVKIHEDLESGALNIIIGTHALLEDTVKFHN
                     LGMVVIDEQHRFGVEQRARLWAKNVKPPHIIVMTATPIPRTLAMTLYGDLDISVIDEL
                     PPGRSKVITRHFRDRDILRVFGFIKKQIDQGRQIYVVFPLISESENMDYKDLEDGLEG
                     YSRAFPPPKYQISVVHGRMKPADKEFSMKMFVDKVTQIMIATTVIEVGVDVPNATVMI
                     IQSAERFGLSQLHQLRGRVGRGTAQSYCLLMTADELSENAYDRISTMVNSTDGFEIAE
                     TDLRLRGPGNLEGTQQSGFTLDLKMANLSQDGQLVQYVKDIAEEILTNDPQLNRNENA
                     IFKIIIKQMNKGFEDFGSIS"
     gene            41983..42759
                     /locus_tag="PKN53_02225"
     CDS             41983..42759
                     /locus_tag="PKN53_02225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD87802.1"
                     /translation="MKHYKRLFPNISYFFKQKHNNILPDTFNSYILNKNIKVDNFILG
                     NKYRNEVLSDNTEIKIKDFGAGSQKLGKETRKIKNIAKISGSKLNFGLFIQKLIKTFE
                     IKSALEMGTSVGIATIFMSYASENIQIDGIEACPETANYLKSKIKERNIKNINIYNND
                     FDSVFENQELNNKTYDLIYIDGNHRGEYLLKYFKILKEKHCSTPCIIIVDDINWSNDM
                     FEAWRELTKTKNATFLNFYRFGVIFLGHNLKHGVFNVKFV"
     gene            42780..>42966
                     /locus_tag="PKN53_02230"
     CDS             42780..>42966
                     /locus_tag="PKN53_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD87803.1"
                     /translation="MNKIIELKDIIKDYYVGSITVNALKGINLNICKNEYVAIMGPSG
                     SGKSTLMMHLIACITSGK"
BASE COUNT        14642 a         6732 c         6096 g        15496 t
ORIGIN      
        1 ttttctctgg aacttgaaac atcagaaaaa tcatctaatt ccatagtcat tttaatcata
       61 tcatactcta tattaagctc atcgagtaat tgcgaaaaac gacttggcac ttttagtatt
      121 gccaataata aatgccctgt atgaacttct tcgctattta gagatattgc ttccgaatac
      181 ataaattttt gaatacgctc tgttgtcttt gttggaacaa ttttatccat atcgtccata
      241 gtaaattgat tattcacatt tttcacatga ttttctattt taaattttaa ctcaagcgta
      301 ttgatatcca agcttttaag aatttccata gcatcgcaat ttggttctcg taaaatccct
      361 aaaaacaaat gctctggacc taaataatcg ttaccaagtc taattgcttc ttctttgcta
      421 tattgcataa catctaataa gttttgtgca aattttctct tcataaatag aataatttat
      481 atataatata aacttataac ctcaaataat attttgcaaa tataatcaaa aatacaatta
      541 gtttaaacta ataaacattg aatttttaaa acaatttaaa agccaaatat tatgccaaat
      601 tatacacgaa aacatagtaa aaatcaacta atatcaagtt ttaagaattt tttagattta
      661 aaactgtttt ttacttgtaa taatgtgata aaattgctta ttttgttaga taataaaaaa
      721 tggaaaagaa atgaaaattt taataaagaa tattaaagaa ctcgtaggag ttgacaacaa
      781 agccagatta cttgtaaaag gtgcagatat ggcaaaatta aacaccatta aaaatgctta
      841 tttattaata aatggtgaaa ttattgagga ttatggaaaa atggaagatt tatctaattc
      901 cttacttgat aatggtaaaa ttaaagaaat tgatgcttct ggtaaaatgg tttttccatc
      961 tttttgtgat tctcacactc atcttgttta tgctggaagc cgtgaaattg aatatagtga
     1021 taaaattcgt ggcttgagtt acgaagaaat tgcaaaaagg ggtgggggaa ttttaaattc
     1081 tgccaaatta ttgcataaaa cctctgaaga tgagctttat agacaagcaa tgcaaagagt
     1141 taatgaaatt atctctcaag gcactggtgc agttgagatt aagagtggtt atggtttaaa
     1201 tacagaagat gaattaaaaa tgttgagagt aataaaacga ataaaagaga caactccttt
     1261 aatagtaaaa tcaacatttt taggtgctca tgcatttcca acagaatata aagacaatcc
     1321 tgacgaatat gtggatttaa ttataaacga aatgattccg caagttgcag cagaagggtt
     1381 agctgactat gttgatgttt tttgcgacaa aggatttttt acagtagaac aaaccgacag
     1441 aattttaatg gctggaatta aatatggaat gcgtccaaaa attcatgcta acgaattggc
     1501 aaaatctggt ggaattcaag ttggtgtgaa atataatgct ttatcggttg accatatcga
     1561 atatactgaa gaagaagaaa ttcaagcatt attaaattcg gaaactatgc caacaatttt
     1621 gccaggagct gcatttttct taaatatgcc atattcgcca gcacgaaaaa tgatgcaagc
     1681 aggcttgcca gttgcgattg cttcggatta taatccaggt tcatcgccat caggaaatat
     1741 gaagcttatg atgtcgtttg cttgcgttaa ttataaaatg ttaccagaag aagcaattaa
     1801 tgcaactaca ataaatacag catatgccat gggcgttagc gatgaagttg gaagtatttc
     1861 taaaggcaaa attgctaatg tttttataac aaaagaaatt ccaacatacg aattttttcc
     1921 atatgcatat ggttcagatt taattgatac agtaatttta aaaggtaaaa tacggtaaaa
     1981 tttcgtaaaa atttaaacat tattaatttt aaattgttgt acaagtatta taaatcaatt
     2041 aaaaaaaaag aattatggaa aatagagatt taattttaaa agttttaaaa ggaaaagaaa
     2101 tgaaagctgg agaaattgct gaacaaactg gcttagataa aaaagttgtt gataaagaaa
     2161 tgaacgctct taaaaaagaa aatttaatta tttctccaaa aagatgttat tggactgcta
     2221 aatagtggct ttaattgctt gtggcagaat aattatatac aattatatag gattatttta
     2281 aaattaaaac aaatgtaata taatttatat tatgttaaat agaattaatt gagttaaaag
     2341 ttaataaagt ttgtaaagtt gaatagtttt agctctattc tgctataatt tgattgttta
     2401 tttcatttgt tttaaaccaa taataaaatg cttcaatatt tgtttttttc aatttattta
     2461 attttgttga cataggattt tcaatgcttg cattctttaa gtctgaaata acgctcctat
     2521 caaaacgctc tattttagtt aacttatttg caattaaagt tgatagatat gctacttttg
     2581 aagcttgaag aactgctgta tcaagtacat atctttcgct gtgaataaaa ctttgtattc
     2641 gttttatgcc atcttgtaaa agtaaaaaat gttctttgtc ctcttgacct tttaagcaaa
     2701 tacaaagtga agtttgaaag atatcatcta aaacttgctg aatgttagtc atatcaagat
     2761 ttctatatgc caattcaact tttgcaaacc tatgaaaagt gttggttgta acagttaaat
     2821 cattaacaat atcaaataaa gaagcaatat cataaagctg ctttataatt tccatagagc
     2881 ataatctatt attcttataa tatggtatcc ctgtagtatt tggtgcaaat gctgtaagct
     2941 tgtcccctaa catatcttct acactaggta ctagaacaaa aatctgtttg ccttcagttt
     3001 ttagaaattt gctttctata ggtaatgata ctaaattagt gtagtaattg tcatcaaaca
     3061 ttacatcaag caatatataa tcttttttta tatttgttct ataagtaact tcatagaaga
     3121 atttcacatg acttttaggt attaaatttg ttgttttgcg ttcaataagt ttaatttcac
     3181 gaaaaccata tttttcaatg tttttactta tatatttttc gatatctgtt tctggtggac
     3241 agataatgtc aatatcaata gacaagcgtt ttgaactgtc taaatgtaac attagtgctg
     3301 ttcctccctt gaatataaac ggacaacctg ataatgctaa tgattccaat aaagacaatg
     3361 ctctaacagc tttttctagc attgttatat tagggaaatt attttcacgt gcaattcttt
     3421 gcaaccaatc tattgataaa ctttctggta gtatcataat gcatcgttat atagtttttt
     3481 aacttgatta agacgacctc ttcgagtagc atagcgaaat aattttcttt tgttgatttt
     3541 cttgctatcc aatacatttc tgtaaaaata taatatctca gttccttgta aaaaatcaaa
     3601 ttcaatatcg tctactatgt ccactaaaat tttttctaaa gttggaactt gcatattatt
     3661 caaacttagt aatggtgatt ctgatattaa tgctctaatg attacagctt ggttccctac
     3721 aatataacgt tgatattctt ctttactagg acgaaaaaat acaggtctat gtaactcatt
     3781 ttttaaaaag taaaatatag cttctgttgc ctcccgctcc acgtcaatgt aaatataatt
     3841 gagttcgggt atgtggtgca taaaaggcat tataaaatcg ctactccaca gacaaaaatg
     3901 aacaaaagga aattgctctt taagttttga attaatttct tccatctcgt ttgaaattgg
     3961 caaataagat gctggtaagc gataaatgcc atgccctatg cgttctaaac gattggcttt
     4021 aacaagtctg taaagttgtt gcgatagtga attgtcagaa agatcagaaa gatattctaa
     4081 tagctcacta cgagaaaacg aaagtctgcc tgctgcaaaa tttaatattg aagtactaat
     4141 actcattgta aaatttttta attataatgc aaatatacta cattatttgg caataaacaa
     4201 aaatattgcc aaaaaatgta gaagttttaa taaataaaat taatttttta caaacttttt
     4261 atttacaact ccttttgttg tattaatttg aattacatac acgccagaac ttaaattagc
     4321 aatatttagg ctcatattat ctgaatcgta aaaactttcc tccataagta tttgccctct
     4381 taaatcgagt attttaacgt gcttagctcc ttctacagga cattctaaac gtaacacatt
     4441 gttagctgga ttaggataaa gagaaatgtt attaatttct ttaaattcaa catttgaacc
     4501 agtgataaac ataaagtata attttgttcc acttgctaca aaagcggttg tctttcctgc
     4561 ataatcagac atatttacag gaccaggacc accataaact ctttcctttt ctccactttt
     4621 tatattgaag cagtagtttt cagctcccga aaacaacatt aaattctcgt tgtctaatgg
     4681 gaaaaaactt tgaacagaca tgtcaaaaat aattgagtcc attttttgat cttcttcctt
     4741 ccaaagatat agcttgtcaa aagaatcagg taagaaaaag aaaattgatt tatcatgtga
     4801 agcctttatt tcatcaaaac tatttatttt cccaaaatca aataaaactt ctaggtttac
     4861 accattatat ctttgccata gccctttctg ccctcttttt aaaagtaata gaatattgtt
     4921 gctacttttt gcttccataa actcacagga gtaagtgtta atgtcgtgaa aattatacat
     4981 ttcagttaga gttttttcat ttgggttaaa gagccaaata tattttttct tattactgtc
     5041 atgcccccaa acgtaaatct cattttcatt tagaacgaat attttagtaa attcttggtt
     5101 ttgattttca aatgattttt cccaatttat gctatcgcta tgccatgtgt aaatataata
     5161 atagcctgaa gtctcaccac tgtaacgata cttaatacaa gcaacaaaaa gatattcatt
     5221 gcttttttca actccagaca ttaaattaat atcaaaatca ggacacaagt ataaagtttc
     5281 cccatcgtaa taataagaag cattattata atctgttgtg gcaaaatatt tattttcaaa
     5341 agcatatagt ccagtaaagt gctgattatc agattctaaa gcactactaa aactaaagct
     5401 aaaaccactt tgagcattta agtttaacgt taaggataat aatgcaaata agtaaattat
     5461 ttttttcatc atgttaattt ttatgtttta ataaaataca aatatatgtt tttcttacaa
     5521 aatccttgca atattcttat aaatatcttt tgaatcaagc aatttaaaaa gtacattata
     5581 atctgctttt atggtttgat agttttctcc ttttggtaag attttgtata attcttgaat
     5641 taaattgtct ttatttaagt cgttctgtat aagttcctta actgatggtt tgtcaagtat
     5701 tagattaact aaagaaatgt actttacttt agctacaatt ttagctattg ctacagaaat
     5761 tttgcctcct ttatagcaaa caacttgtgg taaatcaaaa agtgctgctt ctaaagtcga
     5821 ggttcctgat tttattaaac cagctgtaga attagctaaa atatcataag ttttaccaaa
     5881 taataatttt acattttcag attttatgtt ttgtttgtat aaatctattg gaattgttga
     5941 cgtgcctgca attacaaatt catattcagg aaatttatca acaatttctg tcataattgg
     6001 taaaatatgc tttatttctt gctttctgct tcccggcaaa actgctattt taggcttatt
     6061 gctcaaatta ttttcggcac aaaattcttc aaagctcgtt ttttcatggt ttttaattgc
     6121 atccatcaat ggatgtccaa aatacttgac ctctacccca tattttttgt aaaattcctt
     6181 ttcaaaaggc agtattacaa tcatttggtc gatgtaatcg cgaattttat aagctcttga
     6241 acttttccaa gcccaaacgt ttggcgaaat aaaataaaca gatttatagt tgttcttttt
     6301 tacccatttt gccattctta aattaaagcc aggataatca atgaaaatta ctgtgtcagg
     6361 attaaattct ttgatttgtt ttttacagag tttaaagttt ttgagtattg ttctgagatt
     6421 ttctaaaacc tcaacaaagc ccataaatgc aagttcgtta atatgttttt taataaaagc
     6481 tccttgttct tgcattaaat ttcctcccca agcctgaatt tcggcattag gttttaagag
     6541 ctttaattct tttattaaat gcgagccatg taaatcgcca gaagcttctc ctgcaataat
     6601 gaaatacttc attacataag ttttataaca actacaaaca gcgtatatat cacaactgtt
     6661 gcaattactc ctttaattgc ataccaatag ttaagttttt gcaataaaaa gaaaaacaat
     6721 aaatttggca ataaacaaat acttaaaact gcaattaatg cttcgttttc tattaatata
     6781 tcaataaact tttttagact catttcgttg tttattgaca gtttatacat gtaataaacc
     6841 aaaaaactaa ttattggcaa tatcattcct aatgcaaagc caaaccaagt tttatttaat
     6901 ttttttgttt tatttgtatt cataattaaa ttttaaactt ttaaacgtga ttttaaaatt
     6961 tctattgtgt tatattcagt aaaatcaatt tttgttggta caattgccgc ataattgttt
     7021 tttaaagccc attcgtcggt ttctggatta tcgagttggt gatttgtaaa ttcgcctgta
     7081 agccaataat attctcttcc ataagggtcg tggcgttttt cgtattcttc tttccacact
     7141 ccttttgttt gtgtacaaat tttatatcct ttaaaatctt ttttatcaat tactggataa
     7201 tttatattta aagaaacttg ataaggcaag ccattttcca acacatcttt aattataaca
     7261 tctgcatttt catctattaa atcaaaattt gcatctttag aatggtctaa aagcgaaaaa
     7321 ccaatagcag gaattccgta caaacttgct tctattgctg ctcccattgt tcctgaataa
     7381 attacattaa cagccgagtt tgagccatgg tttattccag ataacattaa gtctggtttg
     7441 cgtggcacaa gttgatttat ggctaattta acacaatcaa caggtgtgcc ttttattgca
     7501 aaaaacttaa ctccatcttc tctttcaatt ctacgtactc taagcggttc gcctaaatta
     7561 attgaatggc tttttccaga ctgttctctt tctggtgctg caacaaaaac atcaccgaat
     7621 tttttagcta tttcaacaag gtgttttaaa cctttggcat ctactccatc gtcattcgac
     7681 actaaaatca actttctatc tttcacacta tatcttttta aaaattattt gtcgcaaatt
     7741 taataaaatt attattattt catattttgc taatatatca cttgtttttc ttttttggct
     7801 tttcgttttt ctcttgcttt tttgaaagtt attttatccc ataaactttt tactaattcg
     7861 ccaaaagtat caaaatcttc agtatagaac acccccactc cgcttgtata aggacctaat
     7921 tcagcttgtg tttcaggatt tactcttgta aaacctttta atctaaacga accttgttga
     7981 ttcatcttta attgtacttc tatatcacca ataagattgc ttgttgttga tttattttcg
     8041 cctactccaa cattaccatt tattaataat ctgtcgttta agaaagttgt tgataaagcc
     8101 acttcaagtt cacgttccga aacttctgaa ccaggacgat aatttatacc tatatcaaaa
     8161 tctttactca tttgcgataa ccagttgctt aattgatttg acaataattc tgctgaagta
     8221 actccaaaag cattagttgt atttccaatt tgagggctta cattttcagc atctgcacta
     8281 taaaatctat tcaaaataag taaatacaac atttgtttat ttatttcgtc ttgagtcatg
     8341 tttgctaatt gtgtttttac cttatcgttt gcatttggta tttctatgct aaacttaata
     8401 tcaggagaca ttaaacttcc cgacatgtgc atatgacatt ctacattagt aggtttttta
     8461 taaatatttg aagagtccgc tactgctgcc attaaatcaa ttagcggagc ttttgcatgg
     8521 taaacagcat ctaaatctaa ttgagcatca tacgcatcac cagaccaaac tattgtgcct
     8581 ccaggtttta tatggaattt tttattgata atattttcca aagtaaataa gtaatcaccc
     8641 gaaacgattt ctatatctcc atacatgtaa aactcctcgt cagaagtata ttccattttt
     8701 aaattacctg aaccatagcc ttttataagg tctccaactt tagagtcgaa cacaatttgt
     8761 gcttctgcat ctggagtaac ttcaacatca agtttaaaat aatactccat atcagttttt
     8821 atagctttag tttcagtttc cccttcttca tcttcttttg gtcgttgtat atatgtaaca
     8881 taacttacat cactaccttc atacgactca gtcataggta atacaaacac agtattaggt
     8941 tctgtttttg caactacatc aatgccaaat ttatctaaat ctccagctat tctaacattt
     9001 cctgttgcaa aagcagttcc atagtaaaga ctattatctt tttcagtagt gttcataagt
     9061 ttcatattag tagcatctac caaaatatca aatcttatat cactaaaatt cttgtgatat
     9121 aaaccaccat ttataacacc tttattatta tgttcatcgt aaattttaaa atctttgaaa
     9181 gaaattgcat cgttagtaat aaatatttta tcattgataa acgcatgtaa ttttaagaaa
     9241 tcataaacta actcggcttt ttgaaattca atatatccag aaatatcagg ttgtgcaagt
     9301 tttcctctta cgtcaactac aacgtctaaa tgtcctttta aattactaat atcaaaatct
     9361 gaagtatatg gctctaatat ttctaaatta aaattatcca taagaaaatt tacgtcaagg
     9421 ctatctgttt cagccatata aacgccacgt attttcataa atttgttcac tccgtcaatt
     9481 ttaaagccgt ttttacgtcc ttcccagata gctgaaatat caaaacgtcc aaaatcttca
     9541 ttattaattt taaagtcatt tatacttact gatgctttta aacttggatt atcataaata
     9601 ttagcaactc ttccatttcc agaaagcgct ccctgtactt gataacctgt aaaagcaaga
     9661 tatggattaa agttcgttat atcaacatct gaaattaaaa agcgtaaaag tttagttgga
     9721 ttttcagaaa tttccccatc aatatttaac atttgatttt cgtgaactaa accaaattta
     9781 ttgatagtaa tatttttgtc atcaacaata acgcttgaag gtcttacctc ccaatttcta
     9841 tttgcaataa ctaaatttga ttcaaaaata gaactttcaa ttttaggcat tgctctgtct
     9901 tttacaggaa taaactttgt taaaatattt atattaccag aattatttat atcatcaaaa
     9961 ttattccaat ttaattctag taacacacta tcttgttgaa cacctaagtt taaaacaata
    10021 ttttctaacc atggaaaact tttgctacta atttcttgac ttgttaatcc aatatttaaa
    10081 agttttgaat ttcctacaac attcaactca gaaccccata aataaatgct gtcatatttg
    10141 attacaggga aactgaaaac catatcaaat ttttcctctt taccattaat actaccttcg
    10201 gcaaaaacat catcatcaat tactaaatcg ggcataaaga gttttagaac ttcgcttaaa
    10261 ttttttaatc ttaaatcaaa tttagcagtg tttgattttg tgtcgtcctt tacttcgata
    10321 ggttttatat atgacggagc ataatgcgat agcaagttat atactctaac tccgagttca
    10381 tcaaactcaa aatcgccggc aaattttaaa tcaatataat cagattttaa gtttaaacaa
    10441 tgcatttgtt tttcatcaat aaaagattct aaattgaaat gttcaagttt tactgcttgt
    10501 ccatttaaaa caagctttaa atcgttgata tttaagtttc ctagaggttt tgtgcttaaa
    10561 ggaccttcta cgtcggcaga cataacaaat ttaatctctg actcaagact atcgttagtt
    10621 atgtgcaaag cattgagatt agcataaagg tcagtagaaa agttaactct ttcggtattt
    10681 ctacctaaag catattgtcc aagaaattga aatttaaaat ttggatcatc aacgaaaatt
    10741 tctccattaa acagagtatt tgaaatatct ccatttatag taatttcaga atatttgtat
    10801 tttaaaatat ctagttctga aactttagat tttatatttg cattataatt accgagagaa
    10861 tcaagattac cagctattat ggcattcata cttatttttc caaaagtttc actatctcca
    10921 agcagttttc ctgcatctac agacattaaa ttaattttac cactataatc aaggtcaaaa
    10981 ctttcaaaat cagtaactaa tgcaatgtcg gttcttatag agccaagtgg tgttctaaac
    11041 tctccataag caactaaatc acttaaaaaa cctgtaatat ttccattaaa agataaatta
    11101 tcaatatcgc ttacaattga aggcagaatc atagggtttt cggggtcaat aattctcaaa
    11161 agcctatcta tatccctttt atttattctt aattcactta catttccaaa tataaatgcc
    11221 atttcaggat tatccaagcc atcaatatct atatctgttg aaaatcttgt gttctcgcca
    11281 taatttagat taaaatcagt taatttaagt tttgaaagat ttccattaaa atttcctgat
    11341 aaataaattt tatctcgcat aaacctatat tcaggcttaa ttggggctaa atctgcaata
    11401 ttgaaaatta atgtgtcgat atttatttta agctttgctt tatgaattgg atctgacaaa
    11461 taatctgtag aatcgcctga gatagcaatt tcagaacaaa taaattccga aagtttagtt
    11521 ttaaaaacaa aatcatttaa ttcaattcca gtatcataat attttaaatg tcccgcaatt
    11581 ttttcaatat ttaaaccaga tttttcagca aaactaaagt tgtttaatct tagttgtatt
    11641 gtgtcagctc catatttaaa atcactaagt tcaagattaa aaccagaaat ttcggcatcg
    11701 gaataattga attcgtcttc aatattattt aagtatctta catctttata ttttaatctt
    11761 gcattaacaa tttcaaaatt attgcaggac aagctaaagt ctgtctcctc gctatctgat
    11821 gttgtgtctg gatctgatgc aaatttttct aaaatacttt cgtaatttgc aactcctaaa
    11881 ctgtcaacct gaacacttat ataaggattt attaaagaaa ttttctctaa attcaaatcc
    11941 atattacgaa ggcttattga agcaattttc gcatttattt cttgagaaca cagtaaagtg
    12001 tcattttgtt cgtctaataa tgtaatattc tgtaaaacaa tagtttttgg caattttata
    12061 taaacattgt ctatgcttaa agctatatcg taatgttttg caaagtaatc tatcacaaat
    12121 tttgttaatt tactttgtaa aaacgctgaa ttaattagaa taataactat agctaagtat
    12181 ttaactatta gcaaactaag tattagcaaa aatcgtttta ttctaattct attcaatttc
    12241 taaagtttta atcaaataaa aaacatttta attaatttac aaatattcaa aaaaataatt
    12301 gaataattac aattaagatg tacttttgta aaccttttta tgttgataaa atgaaaacta
    12361 ttgttttagg catagaatct tcttgtgatg acacttcagc agctgttgtt tgtgatggga
    12421 aattatattc aaacgtaata aacacacaat caattcatta taaatatggt ggtgttgtac
    12481 ctgaattagc tagcagagca catcaacaaa atattgtgtt agtagtaaaa gaagcattag
    12541 agcaagcaaa aataagtttt gatgatattt ctgccatagc ttttactcaa ggtccaggtt
    12601 tattaggctc tttgcttgtt ggttcttcat ttgctaaagg tgtggcaata gcaaaagata
    12661 ttcctattat aggcgttaat catttgcaag gtcatatgtt tgcacatttt ttaagggaaa
    12721 aagatgaaga agaatttatt ccaaaatttc cgtttttatg tttgttagtt tcgggcggac
    12781 atactcaaat tattgttgtg gagtcatata caaaatacga aattattggc tcaacaattg
    12841 acgatgctgc tggcgaagct tttgataaat gtgctaaagt tatgaattta ggttatcctg
    12901 gcggaccatt gattgataaa tatgcacaaa ctggaaatcc aaataaattt aagtttagca
    12961 aacctaatgt tgccgattta aactttagct ttagtggctt aaaaacttca tttttatatt
    13021 ttttaagaga ccaaataaaa gaaaacgaaa attttatttc cgaaaaccta cccgaccttt
    13081 gtgcttcttt gcaatttcaa atagttgata ttttattgga aaaattatca aaagctgtaa
    13141 aacaaactgg aattacagaa attacaattt ctggtggcgt ttcggcaaat tctggcttac
    13201 gaataaaatt aacagaactc gcacaagaaa aagattggac tgttcatatt ccaaaaatta
    13261 gatatactac cgacaatgca gctatgatag ctttaattgg atattatcat tttttagatg
    13321 gaaagactga tggacttgat attgtatctg ttgcaaattt agcacaattg agttaaaagt
    13381 gatacactga acgtagttga agtgttaaaa gttaaaagcg atgcactgaa cgtagttgaa
    13441 gtgtcgaaag tggattttag atttaattca gctaatagat gaaatttttt attaaatttg
    13501 caaacattaa tttttaaaga ttatgaataa agaatttgct aatccttggc atagtgtttc
    13561 gccgggtaaa gatgcaccag aaattgtaaa tggaattatt gaaattccaa aaggtaatcg
    13621 tgcaaaatac gaattagaca aaaatagtgg tttgttgaag ttagacagag ttttatactc
    13681 ttcggtttat tatccagtaa attatggatt tattccacaa acttattgca acgacaaaga
    13741 tccacttgat attttaatta tggctcaaat cgaaattgtt cctatgtgca ttgtgccagc
    13801 aaaagttatc ggtgtgatgc gaatgttgga cagtaacgta gctgacgata aaataatagc
    13861 tgtaagtgct ggcgacccaa gtatgaatca tattaatgat atttcagagc ttcccgacca
    13921 ttttatttct gaaattagaa acttttttga agaatataaa aagttagaaa ataaaactgt
    13981 agtggttgag gaatatttag ataaatatac tgctatggaa attattaacg agagttttga
    14041 agaatacaaa caaaagttag aatttttaaa gtcttataaa ttttaaactt ttaaattatg
    14101 aatatttctc taaattggtt gaaaaactat ataaatatta gcgacgaacc cgaaactatt
    14161 gcagcagatt taacaagttt aggtttagaa gttggaacca ttacagaatt ttcatcagta
    14221 aaaggtggat taaaaggtct tgttgttggc gaagttttaa cttgcgaaaa gcacccaaat
    14281 gctgataagc ttagccttac aactgttgat attggttctg gagaaattca aaaaattgtt
    14341 tgtggcgctc ctaatgtggc tgctgggcaa aaagttatag ttgcaactgt tggaacaact
    14401 ttatattcta atgacgaatc ttttacaata aaaaaagcaa aaattcgtgg cgaagaatca
    14461 aatggaatga tttgtgccga agatgaaatt ggaataggaa gttcacatgc aggaattatg
    14521 gttttaccaa atgatactcc tgtgggtttg cctgcaagca agtattttaa tatagaagaa
    14581 gatgtagttt ttgaagttga cctcactcct aaccgcatcg atgctgcaag tcatattggt
    14641 gttgcaagag atttggctgc ttttagaaat ataaaataca atattccaga tgtttctaaa
    14701 tttaaagttt caaaaaatac atattcaatt gatttagaag ttgcaaaccc cgaagcttgt
    14761 cctcgctatt ctggaatttg catggataat gtaaaagtta cagattcgcc acaatggttg
    14821 caaaatcgct taaaagcaat aggcttaaat cccataaata atattgtaga tgttaccaac
    14881 ttcgtaatgt ttgaaacagg acaaccttta catgcttttg atggcgataa aattgcaaat
    14941 aataaaattg tagttagaac tgttgacgaa aatcacaaat ttgtaactct tgatggtgtt
    15001 gaaagaagtt tgaaatcaac agacttaatg atttgcaacg ctgtagaacc aatgtgtatt
    15061 gcaggagttt ttggtggttt agattctgga atttctaaaa atactaaaaa actttttatt
    15121 gagtcggctt attttaatcc tggttgggtt agaaaaaccg ctaaaactca cggactttca
    15181 acagattcat catttaggta tgaacgcggt gcagatgtaa atataacact ctatgctgct
    15241 aaacgtgctg ctatgcttat tgaacaaatt ggtgctggcg aaatttcttc agaacttaaa
    15301 gatgtttatc cacataaaat tgaaaataat attgttgatt tttcttacga aacttgctgt
    15361 aagctaattg gaaaagaaat ttcaaaacca gaaatagaca aaattttaac agctttagaa
    15421 attaaaattt tagaaagtaa agacgataat ttaaaattag aaattccttc ttatagagtt
    15481 gatgtaacac gtgaagctga tgttattgaa gaagttttac gaatttacgg ttacaataat
    15541 atcgaaaatc ctgaaaaagt ttcgttttct gttcttaatt cagaaaaact tagtcttgat
    15601 aaattacaaa atacaatttc tgatttattg gtttcagatg ggtttaatga aattaaatgt
    15661 aattctttga ttagtgcaaa atattttaat caagacgata agtcaattgt tagaatttgc
    15721 aatccactaa gtgcagattt agcaataatg cgtcccactc ctattattgg aggtttagaa
    15781 acaatagttt ttaacgtaca tcacaaaatt tcggatttaa aattatttga atttggacgc
    15841 acttaccatg caaatactga aattgcagaa ctttcaaaaa ttgaaagata ttatgaatct
    15901 aaatttttag cactttggat aactggaaat aaagatgttt taagttggaa tcaaaaggaa
    15961 aaaccaagcg atttctttac tttaaaatct tatgttacaa aaattacaac tcgtcttgga
    16021 atcgacttgg cagaactgaa agaagatttt gtttcagact caaattttga atatgctttg
    16081 caatataaat acaaagaaat tgttttttgc agttttggaa agttgaatgc taaattagca
    16141 aaatcaacag attgtgataa agatgttttt tatgcagaaa ttaattggga tttagtaaaa
    16201 gaacttgttg ctaaaaacaa aatttcatat aaatctatct caagatttcc aacagtaaaa
    16261 cgcgatttag ccttagtaat tgataaaaac ataagctaca aacaaatggt tgacttagct
    16321 aaaaaaaccg agccaaatta tattagcgaa gttaatttat ttgatgttta cgaaggcaaa
    16381 aacatagaac aaggtaagat ttcctatgct ttaagtttta gtttagatga ccacgagaaa
    16441 acaatgactg acaagcagat aaataaaata atggaaagac ttattaaggc gtttgagacg
    16501 caactaaatg ctattttaag ataaaagtta aaagttttta aagttcgtaa atttggatat
    16561 ttaatatatt atatttttta atgatattat aaagccgttg caaattatta ttttttgagt
    16621 tcaataatta ttctattgtt ttcatatggt ttgctattta gaaaaattgt atctttatta
    16681 tcaacattta tttcaaaata tacatttcca ttatttagtc ttgttaattt ttgaataaca
    16741 tacataccta ttccatcgcc acccaaatca agtttttttg cccaatgtcc agaataattt
    16801 tctgtaaaaa tattttttag ttctgttggc tcaactttca aacttgtcat ttccatttgg
    16861 atttgaactt tttcatcttc ttctttgaat aaaatatgca atgtcgaatg tggcataatg
    16921 tacttagaca tattatcaaa aatatgagac aatattacag aaacagactt gtagtctatg
    16981 gttactgtac catgatattg atcaatatct ataacaatat tccgctccac taaatcaagc
    17041 caaaatggat taagagttag aagtaatact ttatgtatag gatgttttga aaagtccata
    17101 tatggattct cattttcaag catttcgtaa acatcaaatt cagctttcat taaatttgaa
    17161 ttttttaata ttttcaaata tgtataagct gctttactta tatctttttc aattatattt
    17221 ttgatattat caagatgatt attcccattt ctaaaataat cttgtggaaa taatttatat
    17281 agttctagca aaatattcga actatgtgtt attagattat gttttaatct tcgagttttt
    17341 tgactttctt ttgatttttg aacaattaaa tctttttcta aatgaatggc taaaatggaa
    17401 aatgcatcaa gataaaattt aaatatccta tttgttaatt ttttatcata tgaataaagg
    17461 taataattat aactttctgt tttatgcact ccttgtcttc taaattcatt tgtatatttg
    17521 cagagaattc tttcgcaatc gctattacaa tgaataatct cagtttctat ttcttgtacg
    17581 tcactattgc ttacaataat ctgccctaac ttatttgtaa tcagatactt catcatagac
    17641 tagcggtaaa aaaaagttga ataatattcg ctaaagaagc tccattttga agattaaaaa
    17701 catttgcgat attttttgca gaaaaatcct tctttaaata aatagtatta aggtattttt
    17761 gaatcttctc tgattcaagt gtatatccaa attctatcaa tatttgctgt ttgattcttt
    17821 ctatgcattc ttttaatgaa aacgcttctt ttgcataatt ttcaactaca gtttgaaatt
    17881 gatatggatc agcgttttta cttaatcgct tatctgcctt gtcaattcca gcatgaaagg
    17941 cttgtatttt tccttgatct tccgcactat aaacgactac ttttttatta gggtattttt
    18001 cctttaatgc tataataaga ccaagtcctt catctttgaa ttttaatttt ttcccgacac
    18061 cttgtatgtc aacaaatagg atatgtgctt ctcttatttc agtttgatca attgattctg
    18121 catctttaat tctttgtgta ttttgccatc ctgcattttt taatatatca actacaggaa
    18181 aaactttatc atcaatgaac agaatcttac atacactttt gatttcgttc aaatttctct
    18241 ctttaagtac actttttctt tttttccaaa atatcatacg aatttgtttt agtaaataat
    18301 tattattaaa acatgttata cgacacaaaa ataaaaaaaa ttaaaatata aacctaaaat
    18361 tttacacatt attttcaata attattaaca cttttacttg attagagcaa tttaccagct
    18421 ctaattataa atttgagcga atatttaact ttaggtgtag cttgttgaca atagtacaca
    18481 acactccacc cattgagaat tgacataaaa ttaaaactct cactttttta gacgcgtcag
    18541 ccactttcga cttttaactt ttaactttca actgattttt caagtgagtg atagcctcat
    18601 tttccccttt ctcaatagca atttcaactg caagtttggc agcttctaaa agctcttctg
    18661 attgttgttt aagacggaaa ctttctgtta ttttttcttt tatttgtttt tgtatttcta
    18721 tctttatcag aggaatagga atgttttgaa attcattttt attaattgct gttaaaattg
    18781 ttccagaaca attttgtttt aaaatattct gcattggttc agatttgaaa agaactaata
    18841 aagtttcaga attaatttgt ttggaattaa ttacatagaa tcctgtagaa caaagtgctt
    18901 tgttatattt ttctgtaact aaagcacaac ttgatagcga gccttctatt gaggaaataa
    18961 ttacatcatc agcttttaca agtcttcttg ctcttgatgg taaatctact ccccttgata
    19021 ctgtacagtc tgtaatatca cctgatgtac cgatattagc taattcaatg tatttatatt
    19081 ctatattatc ttttggattg taatttttat cttttaattc acaaacagaa gccaattggt
    19141 caaaaccact tgaataattt tgaataagtt ctaaataatc atcatacttc ttctgataat
    19201 attccgcgtc taacctacca gtttctaaaa atgattcttt aaaacttttt atatttattg
    19261 ggtcgctact tagctcaaaa tcagttaaac ctaaggtttc aagtaaaagg gtttcggctt
    19321 gttggtatag ttgtttagat tttaatctac tttcatgagc agtatcaaaa caattcttta
    19381 tttcaagttg tattgattta tttaaaatag gcaataatat ttcttttact tcttcaggat
    19441 tgacattcgt ttgattaatt gattgacgag aacgtctctt tatatcacga attccatatt
    19501 ttgtgtttaa aaaagctgta agatattcag gattaacaat gttttcatca ggaacaaatc
    19561 taaccaaata agatgcaaat gtatattcct gtttttcaac tttacgataa agtccagttc
    19621 taccaaccca ctcataagaa ttagttctat taaataagac atctctgtct tttaatttaa
    19681 atttaataaa ttcttctttt gaaatatctg cacttttttc aacatctaaa tcagttaaca
    19741 tattatgaat ttcattcatt ctataaatag gataaccaac gttgtcttca ttcatagata
    19801 tagaaattcc atattgagcc tttattaaaa cattaccaat cttatcatat ttcaattttg
    19861 ggtttctata aggttcttta gtccaaaaat cactatctat tctaaaatct ttgtttttaa
    19921 aagtttcaga taattcaact tcttctactt ccaacccttc caacaatgct ttatatttag
    19981 cttcgttgaa agggttattt aaaaaaaact tagtcgttct tttttagcga attcaataaa
    20041 tgcttcggca attccgtctt tagtgcaatt atcgtggtta aaaaggtcgt gttttactat
    20101 taaatgtccg tgagagtcaa gtaaatattc ttctaaatcg ttaggcgttg cttgataatg
    20161 gttttgcaca tcgctaagta tatcgctgtc ttctatttca gcagagtgaa aattcccctc
    20221 cgctgccttt ggaaagtctt ttttacgaac aaaaatttta tcgccagaat tatctttact
    20281 tggctcttgc atagttgcaa agaaaattgg gtaatcatca agtttaggac aaagcttatc
    20341 gtcccatttt tgcacaaata aaacacttgt ttttgtacct gtatgtggtt taaatacatt
    20401 tccatgcaaa ccaacaattg ctaaaatacg gcatctttct gcaataaact ctcttattct
    20461 tttgtcgcta ctattattaa aacgtccttg tggcaaaaca atagccatac gaccaccagg
    20521 ttttaaaaaa tcaagattac gttcaataaa taaaatatct ctaccaactt tagtttgata
    20581 ttttccatct tcttttttgc ccaactcata tttagcaagt attcggcttt cttttatatc
    20641 tcctgcaaat ggtggatttg ccataagaat atcaaacgta aaatctctat tactgttttt
    20701 atcagcacgt agctttttca attttttcca gcctaaataa taaatatctt gccaatcttc
    20761 gtcttcagtt ttttcgtccc atctctccca atctaaagta tttaagtgaa gcacattagt
    20821 ttgaccatct cctgcaatta aatttaaagt tcgtccaact ctaacagctt tttcatcaaa
    20881 atctatagca aatactttat cctgcacgta atcaatacat cttggcggtt ttgcatctat
    20941 agtaaataaa tggttcttgt ttaaaccttc gtctttcatt atttgttccc aaacgtgaaa
    21001 aattgtatga acagggaaac cacaacttcc tgatgctgta tcaatcattg tttcatcttc
    21061 ttttggattt agcattttca cagccatatc aattacataa cgtggcgtaa aatattgtcc
    21121 tttttctcct ttactacttt tatttatcaa atattcaaaa gcttcgtcaa ccacgtctaa
    21181 atttgaatta aatagcttaa cgtcttgtag tgacgaaaca caaatagata agtgagatgg
    21241 tgtaagcatt atttttgcat catctgtaaa cacaccttcc catttctgtt tagcattatc
    21301 gaaaagtcct tgaagtttat tttttaattc tgattcagta tcgccgtagt ttctaaattc
    21361 taaatgacga gaaggattgc gaccgctttc catttcgtca aataattttg taaaaataag
    21421 cttaaaaagt tcttcaaaaa catcaacacc agcattagcc aacacttcat cttccatttc
    21481 aagaatcagg tcttttagcg atttacgttc tgttaccaat ttatcttttt caattagatt
    21541 ctcaattgtc caacgttctt ctaaaatatc tgagagtttt tggtcggctc gcggaatatt
    21601 gggaatatct tcaaaatagt tagggtcttt tctatgataa tacgaaattg attctccatt
    21661 actccaaatt ccaattggcg ctccagttgc gttgcaatac gactttaatt gctcctttcc
    21721 ttcttttaat tttggttttt taagctcaac aattatataa ggggtagttt tttgtatttt
    21781 atcaaaaatt acaatatctg cacgcttttt ttcacgtcca aaagtaacag catattcaag
    21841 ttctattcgc gaaataggat aagctaaatc ttgagttaat accattacat aaagttgcct
    21901 tataacctct tcgggtgtaa gttttatttc tttttttcga acaatacaat taatataagg
    21961 ttcaatttta ttcctacttt ctcgaattat aatattttct tcaagttctt tgattttatc
    22021 atcactaaac tgtgttagcc tataattagt gtctcgcaga atatcagcta aagtcataat
    22081 atcttttgtt tgcgttatta aaatgcaaat gtaataattt ttttattcat tttatttgat
    22141 tttttcccag acgcgccagc cacttttaac ttttaacttt taactgacaa agtccataca
    22201 tagcctgttt ttctgtcgaa aaatctaatt attccataat tatttgcgat aattattttc
    22261 catatgtttt ctgaataagt ggctgtatct ctttctatta ctaaaacctc attataaaat
    22321 ttaccattaa ttgtcattgt atcaagtttc ccttctatat aagatgaagc aattcctgaa
    22381 ctaaacacat tatcacttat ttgtaatgag attttacaag caattgaagt gcctgcctcg
    22441 ttattgtcaa tattaaaata atattgcgaa cgttcagtat cagaaaggtg gaatacagca
    22501 tttgaaatgc aatcgtcttc aaaacgtttt ttttctttat atgcttcaga taaatgttta
    22561 ctttttactg tgaaaattaa gctgtcattt tgttgattaa cataaattaa tctatctccc
    22621 acttcataag gcatccatgt taattgtgat ttgtcgaaac aaggacattc tatatttctg
    22681 caactattta taaaaaacat tgcaagtaaa agaaaaatta atgttgatgt tttcatagtt
    22741 tgtttaaatt tcaactaact tagggatatt accaagttaa aattaactct atccagacac
    22801 gccacagcca cttttaacac ttcaactacg agtgcatagc ttttaacttt taactgattt
    22861 ggtgtctagt aaataaaata tggtttataa taatcatcgt aagggcttac aaatcctaaa
    22921 tgtttagtat aaattccacg atgaatagaa tctaatgtat agcgatcaag tttttttgct
    22981 cgataaacat tatatctgct tgccattagt ccaaaaccat cgtttaaatt tgtaaatgca
    23041 ggtttgtcct gtgccaatcc acttgaaggt tcagtaatgt ccatataaat catataattt
    23101 tcgtcaccaa cgactaaatt ataaactaag ctttctggca ttttaacata tcgttttgct
    23161 ccttctctag gtgctggaat attagattgt aataaattat aaaaagctac aactgagaat
    23221 ttgccactaa cttcagaatt gccttcagtt gttttataag tttttgcttg tagccatgga
    23281 attgaataat aagttgtatc atcattataa acctcgaaat aattaaatgt taggcacatt
    23341 tgaaacactt taccattttc tccattataa tatctatatt ctgaatttcc tgcataatgt
    23401 cttaattcaa caggcaatac ttcaactcca gatggcatag taatagatat tccagaaatc
    23461 aactttgttt cgcctgttac aatatgctca ccgtcattaa tatttacctc aagtttataa
    23521 acagcatcat ctctcatatc cattttttga cgccatacaa tatttttatc atttgcaaaa
    23581 taaccttctt ctttgtctat ctctacaggt tcaaatgtcc aagttctagt tttataagta
    23641 tttacatatt cgtgtatgta aacatcaact tttttgtaaa ataatgaatc gctatgctgt
    23701 gccatttcag aagcaggctt attgccaaga aagcttttat ttacttttac atattgatat
    23761 tcagtagatt ggtcaagtac acaatacaca ataggaatat catcccattc tccattcaca
    23821 tctacatcag tactacatga aaaaataaat aaagaaatta aactaataat tattaagtag
    23881 attcgtttca tcttgttata tttgcaaaaa tttaaacgtc aaaattataa aaaatatttc
    23941 atacataaaa aataaaaata tgtctgttca caaagaaatt aaaaaaatca ctactaatgt
    24001 cttgttagag atgaagcgta acaatgaaaa aatttctatg cttaccgctt acgattattc
    24061 acttgcaaaa attgttgatg ctgctggaat tgatgttgtt ttagttggcg attcagccgc
    24121 taatgtaatg gcaggttttg attctaccct accaattact ctcgatcaaa tgatttatca
    24181 tgctgagtct gttgtaagag ccgtaaatag agctttagtt gttgttgatt taccttttgg
    24241 cacataccaa ggaaattcgg aattagcttt atcttctgct attagaatta tgaaagaaac
    24301 cggtgcaggt gctgtaaaat tagaaggcgg attagaaatt gctgattctg taaaacgaat
    24361 tatttctgct ggtattccaa ttatgggtca tcttggttta atgcctcaaa gtattcataa
    24421 atacggaact tacacagtta gagctaaaga agaacaagaa gctaaaaaat taattgaaga
    24481 tgctaaattt ttagaagaaa gtggttgttt ttctattgtt cttgaaaaaa tccctgcaaa
    24541 attaactcaa caagtactta aagaagttga tattcctatt attggaattg gtgcaggacc
    24601 agctgacgga caagttttag tattgcacga catgttagga attactcaag aattttcacc
    24661 acgtttttta cgtagatatg caagtttaga taaaataatg ttagatgctg taaatcaata
    24721 tattgaagat gtaaaatcta ctgattttcc taacgaaaac gaaatttact aacttacatg
    24781 cacaaacaac ttgaaataat ttacgaagac aatcacatta ttgcagtaaa caagcgagct
    24841 tcacagattg ttcaaggcga taaaactggc gacagagcac tttctgagct tgtaaaggag
    24901 tttttaaaag aaaaatactc taagcaagga aatgtttatt taggtttgcc acatcgctta
    24961 gatagacctg tgagtggtgt tgtgttattt gccaaaacat caaaagcttt aactcgatta
    25021 aatgctattt ttaaatctgc tagtgtacaa aaaacttatt atgcgatagt agctaatccg
    25081 cctgcaaaaa cagaagataa attaattgat tatatttata ggaatacaaa gcaaaataag
    25141 tcttatgtgg ttgacagcag caatcaagaa gcaaaaaaag caattttaga atataaatta
    25201 atcgcaaaat cagaaagata ttatttatta gaaataaatc ttattactgg acggcatcat
    25261 caaattaggg ctcagttagc aaatatgggc tgtcctattc gtggcgattt aaaatacggt
    25321 tatccacgct caaataaaaa tggtggtata aatttacact caagaaaaat tagttttata
    25381 catcctgtgc aaaaagaaga aataaccatt actgccccat ttcctgaaga aaaaatatgg
    25441 actattttta atattagtta aaaaccatca gttaaaagtc gaaagttaaa ccacaaaaga
    25501 cacaaaaggg cacaaaagtt aaaagcgatg cactgaacgt agttgaagtg tcgaaccgag
    25561 cttgcgagct caccgaaggt aaagtattgt gttgccctta cgggcaaagc aaatcagtta
    25621 aaagcgatgc actgagcgta gttgaagtgt taaaagtagc ttgcggattc ggctttactt
    25681 ttttttataa acttttaact gtattggctt cacctacgat taattacatg ttgtcctacg
    25741 gacaagcctg ttaaagtata aaaatattgt tctaaagcag tagtagatat tgtagtcaat
    25801 taaggtgttt tagaagtatt caaatgcaag gcgtctgaga ttgagaacga aggagcgtac
    25861 cagaagcgat gcactgagca aagccgaagt gtacgtgact gagtttgaat atcgaaagca
    25921 acgaatcagt tggatactta taaaacaccg ccccttataa taaaaaaaag ttggacagaa
    25981 aaatatctat ccaacttttt ttcatgttat atcaagtcaa attattattt tttagttttc
    26041 ttttcttttt ctaataattc ttctttaata gcagctaagt cggtaacgtc tccaagagtt
    26101 gttttctcaa ttttattttc gatttgagct ttagttcttt ttgttgtttt ttcgccttta
    26161 gcttttttgc tttctgcttc tttttcttta ttcaagtctt cataaattct tgagtgagaa
    26221 acgaaaattt tcttagaatc tttattaaat tcaataactt tgaaatctaa tttttcttca
    26281 attttagctg agctaccgtc ttctttaatc aagtgttttg gagtagagaa accttcaaca
    26341 ccataaggaa gagcaatgat agaaccttta tcgctcattt caactattgt accttcatgt
    26401 atagaatcaa tagtgaaaat agtttcgaat acttcccaag gattttcttc tatttgtttg
    26461 tgtcctaggc ttaaacggcg attttcttta tctatttcaa gaactacaac ttctaattca
    26521 gcacctatag tagtaaattc tgctgggtgt ttaattttct tagtccatga taagtctgaa
    26581 atatgcacta aaccatcaac tccttcttct aattcaacaa atattccaaa attagtaaag
    26641 ttacgaactt ttgctgtttg ttttgttcct atgttaaatt tagtttcaat gttttcccat
    26701 ggatctgggt gaagttgttt aattcctaaa gacattttgc gttcttcgcg gtctaaagtt
    26761 agaataacag cttcgatatc atcaccaact ttcataaatt cttgagcaga acgtaagtgt
    26821 tgcgaccacg acatttctga aacgtggatt aaaccttcaa caccaggagc aatttctata
    26881 aatgctccat agtctgctaa gacaacaact ttaccactaa ctttatcacc aactttcaaa
    26941 ttagcatcta gagcatccca tggatgtggt gttaattgtt ttagacctaa agcaatacgt
    27001 tttttatctt cgtcaaaatc aagaattacc acttgaattt tagaatcaag acttacaatt
    27061 tcttctggat gagttacacg tccccaagat aagtctgtaa tgtggataag tccgtcaacg
    27121 ccacctaagt caataaatac gccgtagctt gtaatatttt taacaatacc ttcaagaact
    27181 tgtccttttt ctaatttagc aacaatttct ttcttttgtt cttcaagttc agcttcgata
    27241 agggctttat gtgatacgac aacattttta aattcttcgt tgattttcac aactttaaat
    27301 tccattgttt taccaacata aacatcataa tcacgaatag gcttaacatc aatttgagaa
    27361 ccaggtaaga aagcttcgag tccaaatacg tcaacaatca taccgccttt agttttacat
    27421 ttaatataac ctttaacgat ttcatcattg tcgtgagctt cgttaacgcg atcccaagat
    27481 ttaagagatt ttgctttttt gtgagataaa attagttgac catcaatgct ttccatattt
    27541 tctacaaata cttcaacttt atcacctact ttaatttctg tttcggttct aaattcgttt
    27601 gcattaatga taccttcaga tttgtagcca atgtttacaa caacttcgcg tttgttgatt
    27661 gctacaacag tacccataac aacttctcct tcttcgatta gcttaaaagt attattgtat
    27721 aattcttcta attttgcacg ctcttctttg gagtaagcat catctttttc accaattaaa
    27781 tcccaattga aattttcaag aggtttaatt tcgggttttg ctttttcttc agctactaca
    27841 gtttctgtgt tcgcctcgtt tttaatttcg tgattttgaa cttcttctgt tttttgtggt
    27901 tcaacattca cattgtcatt aatttctgtc attttttaat aagtttcttt aatttggtta
    27961 gacattatgt taattaaaat ttttagagtg caaaggtata gttttatttt taaaaagcaa
    28021 ttagtttttg caaataaact aaaacgcaca aaatcaagta atttcaattg ttttattaaa
    28081 tttttgaaaa aacaaataaa aactaatcaa aaaaagccga caaaaatcgg ctttttttgt
    28141 gaactaggga ggagtcgaac ccccaacctt ctgatccgta gtcagatact ctatccaatt
    28201 gagctactag tccaaaattg agtttgcaaa tatatattat atttttcatt acacaaaata
    28261 tttttttcaa aaattacatc gattcaaaat tcaacagtat tatttttaaa aaattttcta
    28321 aaacatataa ataaaattct tttgatgaaa tattttcatt atacttgtag caatatttaa
    28381 tttaataatt taatttaatg tttaagatat gaaaacacca atagatgtaa aattagtaga
    28441 tgctcaaatt gagaaattag gcgttaaaga ccttagtaaa gctacaattc gtgagattgt
    28501 tactttagta aattggattc atgaagaatc aggtaaagag tttgtaagaa tggaaatagg
    28561 cgaacctggc ttaacaccac caagtgtagg tattgaagca gaaatcaatg ccttaaaatc
    28621 tggagttgca tcaaaatatc ctatgattag tggagttcca gaattaaaaa acgaagcgtc
    28681 aagatttata aaaaacttta taaatattga tattagtcca aattcatgta tcccaactgt
    28741 aggttcaatg caaggtgggt atgcaacatt tttaatatgt gcaaaccttg atgctaaaaa
    28801 agatacagct ttgtttatag acccaggatt tcctgtgcaa aaacaacaat atagagttat
    28861 gggtcataaa ttcgaaagct ttgacatttt tgaatatcgt ggcaaaaaac tacgcgaaaa
    28921 attagaatct tacctctcta aaggaaatat aaactcaatt atttattcaa accctaataa
    28981 tcctagctgg atttgcctaa cagaagaaga acttcaaatt attggtgaat tagcaactaa
    29041 atatgacgta attgttattg aagacttggc ttatattgca atggatttcc gcagagactt
    29101 acaccaacca ggaaaaccac cataccaagt ttcggtagct aattatacag ataattatgt
    29161 gcttttaatt tcaggttcaa aagcatttag ttatgcaggt caaagaattg cagttgcagc
    29221 tatttccgac aaaatctata atagaaaata tcctaaccta ttacaaagat ttggaacaga
    29281 aggatttggt tatactttcg tttcaagaat tatttatgca ctaagctcag gagtatcaca
    29341 ttctgcacaa tatgctcttg ctgcaatgtt caaagctgct aacgatggca aatttgattt
    29401 tgtaaaagaa gtgaaagaat atggcgtacg tgctaaaaaa atgaaagaaa tgttccttga
    29461 tgcaggattt aaaattgttt ataataaaga cttagacgaa gatatagctg acggttttta
    29521 tttcacaatt tcatatccag gaatggaagg tggcgaatta gtaaaagaac ttttatatta
    29581 tggaataagt gcaatttctc tactaacttc aggaagtact cgtagcgaag gactgagagc
    29641 ttgcgtatcg caaattgttc cagacaaaat gaaattatta gaagaaagat taaaagtttg
    29701 gaaagaaaac cataaataaa acctatggca acacatgtcc tacgtgaaga taaagatata
    29761 aatagcgttt caggattatt acaagaaaaa tatcctcaag caaaatttaa aaatagaaaa
    29821 actaacatta tcattaaaaa taaaatgata atgttagtta ttagacaagg acgtaaatca
    29881 tttaaaatca caggtggttt aaatttttct tacttacccc tactaattct aattattgct
    29941 agcattctat taagtactgc tgccttaatt ctggttttaa taatattaga aataatattt
    30001 agaagaaaaa ttaataaaat taaaaaagat gttgcaaatg ttttgtttgg agcagttggt
    30061 tgattttcag aatgtttata gagatttgtt aaagcattac attaagggat ttaaagatgc
    30121 ttgtaaagat gaaaatgttt tattaaatat tgaactaaaa gaagttcata gtctaaacgt
    30181 agctgaaaat tgcttaaaaa ttgcaaaatc tgaaaattta aaccaagaaa aaacacttat
    30241 cgcctattta tgcggtttgt ttcacgatgt tggaagattt gaacagttta caaaatatca
    30301 cacttttaga gataacgaaa gcctatatca tggcgaactt ggtgttgaag taattaaaaa
    30361 agagcagttt tttaaagatt tgcccaagga aactcaagat attatttgta cagcggttta
    30421 taatcatgga ttgcgtgaaa ttcaagctaa tgtaaaaaat gatgagttat atttttctaa
    30481 acttgtgcgt gatgccgata aaattgatat ttatagaatt gtttgcaaat actacaataa
    30541 ttctggacca cggaatatag ccttagaata tggactcgag gataaagctg aaatttccac
    30601 aaaaccttta aatgacttca taaacaaaaa tttaatttca aaagaagatt tagaaagcgt
    30661 taacgacttt aaattaatgc aaatttcgtg gatttacgat ttgaatttta atttttcaaa
    30721 acaaattatt aaagaaaaag cctatattga cactatttta gacacaataa aaggcgaaaa
    30781 tgtgaaacaa ataataaaac aatacattac tttataatat aattcattaa aaatagttaa
    30841 atttacaaat gtttaatttt ttacttttat agatatgaaa gcatttaaag catacgacat
    30901 tagaggaatt tataataaag acttcaacaa ggaagacgtt tataaaatag gtttttttat
    30961 tcctcaagta ttattgactg acaaagtttt ggttggtaga gatgtaagag agtcatcgcc
    31021 tgaagcacta gaatatttag tgaaaggcat tttggatagc ggtgcagacg tttattacgc
    31081 tggtttagcc actaccccaa tgatttatta ttcaactgca aaatacggat ttggtgcgtc
    31141 tgtaatgata acagcttctc acaatccagc agaatataat ggtttaaaag tttcgggtaa
    31201 aaatgcaaaa cccattggct acgacactgg tttaaagaag gttgaagaat taatgaaaac
    31261 tcaagaaatc attgttagcg accaaaaagg taaatatcac gaaataaatg tgagggatac
    31321 atattttgat ttcttaaaat cttatgttcc aaaattaaat gacttaaaaa ttagcatgga
    31381 tataagcaac ggcatggcag ctctttttat taaagaattg ttaggcgaca aagtaatgta
    31441 tttttacgat gaattagacg gtagcttccc aaatcatgaa ccaaatcctt tagttccaaa
    31501 aaatattgtt gatttacaga aacatgtaat agaaaacaaa tctgatattg gggttatttt
    31561 cgatggcgat gcagacagag tgatgtttgt tgatgaaaaa ggcacattta tttcgccaga
    31621 tttaatgatt gctgttttgg cacattattt ccttgaagaa aaaggcttaa aaggcaaagt
    31681 aattcaagat attagaactt ctaaagctgt gggcgaatat attgcacaat ttggtagcga
    31741 aatggaaatg tggcgagtag gaagagcttt tgctgcacca aaactaaaag aaatagatgg
    31801 aatttttggt ggcgaattag caggacatta ctattttaaa gatttttatt attcagattc
    31861 tggaattttg gcttgtttgc taattttaag agttgtagca caaatgcacg aaaaaggaat
    31921 tacagtttct gaacttatag gaaaaataaa aaaatacgaa aattcaggcg aaataaactt
    31981 taaaatagag gaaaaaacaa aagctatgga tgctgtaatt gctcatttta aagaaaaaga
    32041 aaaacctgat gcattttttg attttgacgg atatagaatt gaatataaaa attggtggtt
    32101 gaatattcgt ccttcgaata cagaacctta tttgagattt ttagcagaag caaatacaga
    32161 aaaacttttg aaagagattg ttgacactgt tgttggaatt gttgaatctt atggtggaaa
    32221 acttgtaagt ggacattaaa atctatttta agacagtctc taaattgtgt taaagtgaaa
    32281 ctttatttta acgattacgt atatttacta ttatataatt gaattatctt gcatgaaaaa
    32341 ctttacagaa gaaacaacaa attttagttt taacaggttg ccgaaagatt tgcaaaaaag
    32401 tctttctact atctatagtc acactgaacc tgttgcaaac ccattgaaag ttaatgctat
    32461 tcatcaaggt gatgctagtg aacttctaaa aaaaatagag ccaaatagta ttgctgtaag
    32521 tgtttggtct ccaccctatt ttgttggtaa aaattatgaa aagcatttaa gttttgaagg
    32581 gtggaaaaac cttttgaagt cagttattgc aaatcatttt tcaattataa aaccaggtgg
    32641 atttttagct ataaacattg cagatatttt ggtatttaaa gatgagaaaa tgcctaaaat
    32701 tcaagctgat gcagttcata gaaagcgaat ttctatttca aaagaaaata ttttagaagt
    32761 tttagctaaa aatcccacta tgaatagaaa tgacttagcc aaacacttta aatgtagtga
    32821 acaaacaatt gatagaagat tgcatgggaa caatgtaaga ggtggaaaac aagaaactca
    32881 atcaagagtg aaaattattg gtggattagt tgaaaattgg gcattagaag caggttttta
    32941 tccttatgat agacgaattt gggtaaaaga tgcagcttgg gaaaattcaa gatgggcaaa
    33001 tttatcatat cgttctgttg atgagtttga atatatttat attttttgga aaccgggcat
    33061 tacaagatat gatagaaatc gtttatctaa tgatgaatgg aaaaattggg gttctcgtgg
    33121 tgtttggcaa tttccgtcag taagagcaaa tgatgaccac gaagctaaat ttccacttga
    33181 attaccaaca cgaataatta aacttttatc tgacgaaaac gacatagtgc ttgattgttt
    33241 tatgggtagc ggaactacag ccattgcagc tatcaaagaa aatagaaatt atatcggaat
    33301 tgaactaaat cctgaatatg taaaactatc aaaagctaat attttaaaag aaactggtgt
    33361 aaattcaatt attacgttca atgctaatta atccaaacac attacaagat aatttagaaa
    33421 gattggaagc aattgaaaag gcttcgctaa gacttgttgt tcaagctatt tatgacttta
    33481 gagaaagtgc aaaagaaata tttgaatttg aaagtgattt agttgctgac attggtgaag
    33541 atataactag agaagcactt gataaactcg gcgtatctaa aattgagcaa aggctttttg
    33601 gcaaaattga ttataaaaaa gcaagatatt tatttcaacc agactttgct attaaacaag
    33661 ctctttttgt tgactcaaaa gccgaaaaga taagtggacg aggtactgca actttacaaa
    33721 catctcaatt ttctttgatt gtaaaacagt tcagagcaaa tgaacatatt gaagttaaag
    33781 gaaagttgcc atgtattatt gacaattctt ttattacaac aactattttc gtaaaatata
    33841 attatgaaga aattaaagga aaaaacattt tgaatgacat tactattgct gcattaccaa
    33901 atggatttct tcaaaacatc tacaatccta ctgcacaaga tacgattttt gtagcaggac
    33961 gaaatgctcc aactcgtgat gaagaattta gggtaagact aagttttagt cgccttaaag
    34021 caaaagctaa atggcgtgtg caaaatatac aaatgtctcc aaatgaattt cattgggatg
    34081 aatagttatc taatctttta aactcgcatg gctcggttct acacttcaac tacgttcagt
    34141 gcatcgcttt taacttttaa ctaactggta ttcaccatta ttatctttct gaatttgtaa
    34201 gtatttcaaa tagttttctt cagtaaattt ttcgtatttt ctaaaatatc cgttgtgaat
    34261 aggtaataat tccaaatcgc ctcttaagac tttatgtgtt gagaaaattt tcttgaaaag
    34321 ccaatttata atttcactat taagcaaatc tgttagctgt tgtgcagaaa tatcaatgtt
    34381 tttaggaatt aggatatttg aactgtttaa aattaaaact tggtttttat cacaataaaa
    34441 gcataaatta gaagtgataa acttataaat tattttttct ttagctaaaa aattttctat
    34501 tggtgcaact tgctgaaatt gcgaaaaatc atttaaaata aaattacttg gttttttaag
    34561 tcctgatttt gtgatgtcag aacctttgta aattggcaaa taaccctctt tttcttgact
    34621 tatgcagaat tttttattat ttcctgttac aatgcctaaa gcccaatctg cattagattt
    34681 taagcttata tgatttatag aatacaaatg attgattatc ttattttctt cgtttgttgc
    34741 ttcaaaattg aaaatacatt ttggattatt aataaaagaa cttataaatc ttgtgtgtga
    34801 tttatcatta ttttcacaaa taatttcatc atttttactt gaagttttat tttcaataat
    34861 tatcgcttta gctttagtaa tcaaactatt aaatgctttg ccataatcaa caaacctcaa
    34921 tatttttttc tttataattt ttcttctaat atcctcgaat tgaccaatat taaaaaatgc
    34981 ttcttgaatt aaaaaaccaa gttttccttt attttttaaa atatttaaac tagctgccaa
    35041 gaacaaagat gttgtatcaa aactattgcc acagttaaat agcttggaat atttttgctt
    35101 atcttgtttg tcaattttct ttccccaagg tggatttgta aaaattaaat cgaataaaat
    35161 attgcttttt ttaagttttg gtgctttttc taaaaaatta cagcaaataa tatttggaga
    35221 ttcatatcta aattcttttt taattctttc tcttgtaatc attacagcat tttcgtctgt
    35281 atcgtagcca taaacatttt caggctttac tcctaaccta attgcttcaa taataaaatt
    35341 tcccgaaccg caacatggat ccaaaaattt aaaatcagga gaaatgtaaa tatttttaaa
    35401 catatctctt actatacaag gcggtgtgta gtaaatccct tctttgtttt tataagaatt
    35461 tgacagtaat ttttcgtatt cagaaccaat atcagcagac ttggtagtgt ctattaagtt
    35521 ttttagtgtt ttacgtactt taacatggtc gtgagaatct tttaaaagtt tatttgagcg
    35581 agaatttaat ttttccttgc ctgcaacttc ttgcataaac ttatcaaaag actttttcga
    35641 aattaatcca gcatttattt tagttaagta tcctgttttt atccagttgc gaattgttgc
    35701 tgtggagaca tttgctagtt ttgtagcttt agctatttta attccaacat ttttagaata
    35761 ttctgaactt gcaaattcca caacttttta ctattttctt acttttttcc aaatttgttt
    35821 ttaataaaaa catctatttg ttctgctgct ttacgtccag cgcccattgc tagaattaca
    35881 gttgcagctc cagtaacatt atcgccacca gcaaacacgc cttctcttga agtttggcta
    35941 gtattttcgt caataataat accgccccat ttttccgtat taagatttgg tgttgtactt
    36001 gcaattaaag gattaggcga agttcccaaa gacataataa ccacatcgca atctatatca
    36061 aattcagaac ctttaattgg tattggtcgt ctgcgtccgc tttcgtcagg ttcgccaagt
    36121 cccatttcta cacaacgcat tcctgtaacc caaccttttt catttcctaa aatttctaca
    36181 ggattattca aaagtttaaa aattattcct tcttctttgg cgtgatgtac ctcttcgact
    36241 cttgctggca attctttttc gcttcttcta tacactaaat acacctctgc tcctaaccta
    36301 cgtgcaactc tagcagcgtc catagctacg tttccaccac ctactacagc aacgcgtttt
    36361 cccacataaa ttggtgtatc atattctgag tcaaaagctt tcattaaatt agagcgagtt
    36421 aaaaattcat tagcagaaac cactccattc aaattttcgc cgggaatatt cataaacttt
    36481 ggcaatccag caccagaacc taaaaatata gcatgatagc cttctttatc aagtaaatca
    36541 tcaatggtta tagtccgccc aacaattacg ttagtttcta tttctacgcc taattttata
    36601 acattgttga tttcgtatct tacaacttct tctttaggca aacgaaactc aggaatgcca
    36661 tattctaaca cgccaccggg taaatgaaga gcttcaaata tttttacttg ataacctaat
    36721 tttgccaagt cggttgcaca agctaatcct gctggtccgc taccaataac tgctacttta
    36781 aagccgtttg taggaacttt tgtaggattg taataatttt cttttcttcc ccaatcagca
    36841 acaaaacgtt ctaattttcc gatatttact ggttcgcctt taactccaag cacacatgag
    36901 ccttcgcatt gcgattcttg tggacaaact ctaccacaaa tggctggtaa acttgaatct
    36961 tcggcaataa tatcagcagc ttgtgcaaaa tctttatttt ttacagctga aataaattct
    37021 ggaattttaa tttgcactgg acatgcctga acacaacgag gatttttaca ctgaatacaa
    37081 cgatgagctt ctaatacagc ttcttcagcg ttataaccat agctcacttc ttcaaaattt
    37141 ttgatacgtt cttgtgctgc ttgttctctt actggaactc taggtatttt tgccattata
    37201 ttatcttcct attttacatt tatgattttt ttctaattct tctctttttt gtccttcaat
    37261 agttctatac atattttggc gacgcattgc ttcgtcaaaa tcaactaaat gagcatcaaa
    37321 ttcaggaccg tcaacacagg taaactttgt ttgtccacct atgcttactc tacaagctcc
    37381 acacattcct gttccgtcaa ccattaaagt gtttaaactt gcaactgtag gaataccata
    37441 ttctttagtt aacaaactta caaatttcat cattatcatt ggtccaatgg taacaactaa
    37501 atcgtatttt ttattttctt tttcaactaa ttctttaagc ttatcagtta ccatgccttt
    37561 aaaaccatag cttccatcat cagttgtgat ataataattg ttagaaactg cacgcatttc
    37621 ttcttcgaga attacataat ttttagattt tgcacctatt attacatcag gtttaaaacc
    37681 tctattgctc aaatatttca cttgaggata aacaggtgct gctccaactc caccgccgac
    37741 aaaaagtatt tttttattag caagttcttc gtcagttgca tgaattaatt ccgaagcttg
    37801 acctaaaggt cctgcaaaat ctaaaatata atcaccttca ttatgttcgc aaattaagtg
    37861 agtagaaaca cctatacact gtgttacaat agttacagtt cctttttctc tatcataatc
    37921 acaaatagta agcggaattc tttcaccttt atctctaact cttaaaataa caaattgacc
    37981 tggttgagct gattttgcaa ctcttggtgc ctcaatatcc attaaaaata tttgttcggc
    38041 aagtggtctt ttctttacaa ttttatacat ctaatttatt atatttttta aattcaacct
    38101 atctgcaaat ataaacagat tttcggatta acaaaaaatg aaaatacaaa atttttcttt
    38161 taaataaata tgatttttat atgattggga attttaatta ccagcttgat tactaggtgt
    38221 tttgcgtttt aattattaaa aaaatccact atttttcttt ctacaattcc tctataaaca
    38281 aatgcatcaa caactttatt ttcataaagg ataattacag gaactccacc agcagttatt
    38341 tgcataactt cttcttttgg catttgaata tactttatgt cggttgactt ggtatcatct
    38401 aaaaattttg caatatcatc agactcaaca aatatataga gtacattact tttatcaata
    38461 ttatgatttt tcaccatgct tgaaattttc tttgcagtta aattacataa tttacagtaa
    38521 ggacttaaaa agcaaattat agtttttttg ttttgtcttt cttttagaac ggggttttct
    38581 ttaaattttt caaaatgttc aacgctataa atatcggtaa gttttgaata ttgtttataa
    38641 tatataaaat ctggtggaga tataatatta actccaacaa tacttataac aaaaatgctt
    38701 atagttacaa gtttcttata tttgaattta aaatattttg aatttctaac ccagactaaa
    38761 cctgcaataa taactgcatt tttaataatt gattgaacgg gacttaaaaa tatattagaa
    38821 ccaaaacatt gacaattttc cacatcacct ccaattgctc taatgcctaa atacactgta
    38881 aaagcggctg tagtaataag ggttaagtca gtgatgaatt tgtataaatt taggcataat
    38941 aatactccta aaaacaattc aaaaccaata attaatcttg ctaataaaat tgataagtct
    39001 aaggaaaata aattaatagt aaaaatatat aattcaaaat catctattgt aaaaagtttt
    39061 aaaatagcag aagctataaa aactaatcct aatatgattt taactattag tttccacata
    39121 aaaaaataaa aaagcttgac ggtgtttcat aagtatcaaa ctgctacgtt gatttcaata
    39181 ttcggactca gtcacgtaca cttcggcttt gctcagtgca tcgcttctgg tacgctcctt
    39241 cgtcctcaat ttcatcgcct tgcattttaa cacttctgaa acaccttgaa aggttattat
    39301 cttaacactt gtttgaaaca ccgtcaagct caaaacttta tttgcattta atttcagtat
    39361 ttaaaacttt ttggtttcca tctgaacatt ttgtgccttt ttctttttca aaagttactg
    39421 aactaatttc ttttccatta atataagttg tacatgtaca ttcttttgaa caactagtga
    39481 aacctaataa tgcaattcct actaaagcta aagcaataaa aatagttttt ttcataattt
    39541 aattatttta tacaatgcct actcttatgt ttttcggctt ccacatccta ctcttttatg
    39601 gattttcgga tacttccata caatattatt atttttttgt cgcttattaa aatcaaaact
    39661 tagtttttaa atctgttgca aatataatac ttataatgat tactcaaaat attttattta
    39721 aataatatac aaaattacat tttattaagt ttttaactta tagaaccaaa gtcttcaaaa
    39781 cctttattca tctgcttaat aattatttta aaaatagcat tttcatttct attaagttga
    39841 ggatcatttg taagaatttc ttctgcaatg tcttttacat attgtaccaa ttgtccatct
    39901 tgactaagat ttgccatttt taaatctaaa gtaaatccac tttgttgtgt cccttctaaa
    39961 tttcctggtc cacgtaatct caagtcagtt tcagcaattt cgaagccatc tgtagaatta
    40021 accattgtag aaattctgtc ataagcattt tcgcttaatt catctgctgt cattagtaaa
    40081 caataagact gtgctgtccc cctacctact ctacctctca actgatgcaa ctgcgacaag
    40141 ccaaatcttt cagcagattg aattatcata accgttgcat taggcacatc aacacctacc
    40201 tcaattacag ttgtggcaat cataatttgg gtaactttat caacaaacat tttcattgaa
    40261 aattctttat cggctggttt cattcttcca tgaacaacag aaatttgata ttttggaggt
    40321 ggaaatgctc tgctatatcc ttctaaaccg tcctccaagt ctttatagtc catattttca
    40381 gattctgaaa tcaatggaaa tacaacataa atttgcctgc cttggtcaat ttgtttttta
    40441 ataaatccaa acactcttaa aatatctcta tctctaaaat gtcgagttat aacttttgag
    40501 cgtcctggtg gaagttcgtc aataaccgaa atatctaaat cgccataaag tgtcattgca
    40561 agtgttcgag gaattggcgt tgctgtcatt acaattatat gtggtggctt cacgtttttt
    40621 gcccaaagtc tagctctttg ttcaactcca aatctatgtt gctcatcaat aactaccatt
    40681 cctaaattat ggaatttaac agtatcttca agtaaagcgt gagtacctat tataatattc
    40741 aaagctccag attctaaatc ttcgtgaatt ttaaccctat ctttcttttt aacagaacct
    40801 ttaagaagtg caacattcac gtccaaatct tttaaaaaat tacttataga ttttaaatgt
    40861 tgttgagcta aaatttctgt tggagccatt atgcaggctt gataaccatt atcaactgcc
    40921 ataagcattg caagaagtgc taccaaagtt tttccacttc ctacatcgcc ttgcaacaaa
    40981 cgattcattt gcttgccact cgctacatct tttctaattt ccctcaacac tcgcgtttga
    41041 gcgtttgtga gtttaaattt tatgttttta aaaaagcaat cttttaaaat tttgtctttt
    41101 ttcctgtcaa aaataaatcc cggtgtattt tgcttacgat ttagttttaa ttgcattaag
    41161 tttagttgaa tataaaaaag ttcttcaaac ttaattctgt attgtgcttt agctaaactt
    41221 tgtgtgtctg ttgggaaatg aagctcaaaa agcgtttctt ccaagccttt taaattatat
    41281 ttttgacgaa accaaggtgg taaagtttca ggaatttgtc ctttaacttg ttttaataaa
    41341 gtatcgcaaa gtttttgtat tacctttgaa ttaatataac ctgttttaag cttttctgtt
    41401 gtactgtatt gaggatataa attacttacc aactgctttt gccattttgt aaattcctca
    41461 atatcagggt gtacgatatt tagtctgttc ccaaaaagtg taggttttcc aaaaagtaca
    41521 tattctttat cgcgagaaat tgaactaatt atccaatttg tagcattaaa ccaaacaatt
    41581 tcaattactc cagtagcatc agtaaaataa cctgttaact ttttcttgtt tctcgaaata
    41641 ttgcttgttt ctaaatgtgt aaattttcct ttaatctgaa tataaggcaa atctttattt
    41701 agttcattta tattgtaaaa tttgcttcta tctatgtagc gataagggaa atagtacaac
    41761 aaatcattcc aagtggttaa acctaattca tttttcaata attcggctcg cttaggtcct
    41821 acaccaggca aatatgttaa ttcattatct aaaatcgcca ttttaaacaa attgtctgtc
    41881 cacaaaaata aaaaaaatat tgaaataatt tataaatata acatttttaa aattaagatt
    41941 ttatgtatct tgcggataaa ataaattgaa acttatttat aaatgaaaca ttataaacgg
    42001 ctttttccca acatatcata ttttttcaaa caaaagcata acaacatatt gcctgataca
    42061 ttcaattcct atattttaaa taaaaatatt aaagtcgata attttatttt aggaaataaa
    42121 tatagaaatg aagtgttaag cgataataca gaaattaaaa taaaagattt tggtgctggt
    42181 tctcaaaaat taggaaaaga aacaagaaaa attaaaaata tcgcaaaaat ttctggttct
    42241 aaactgaatt ttggtttatt tattcaaaaa ttaattaaaa cttttgaaat aaaatctgct
    42301 ttagaaatgg ggacatcggt tggaattgca actattttta tgtcttatgc ctccgaaaat
    42361 atacaaatag acggaattga agcctgtcct gaaactgcaa attacttaaa gtcaaaaatt
    42421 aaagaaagaa atattaaaaa tataaatatt tataataatg attttgactc agttttcgaa
    42481 aatcaagaat taaataacaa aacctacgat ttaatttata tagatggaaa tcatcgtggc
    42541 gaatatttac ttaaatattt caaaatttta aaagaaaaac attgctctac cccttgtatc
    42601 ataatagttg acgatataaa ttggtctaac gatatgtttg aagcttggag agaattaact
    42661 aaaactaaaa atgcaacttt tttaaatttt tatcgctttg gtgtaatttt tttaggacat
    42721 aatttaaaac atggagtttt taatgtaaaa tttgtttaat ttcgtaaaaa ttttgtaagg
    42781 tgaataaaat aattgagcta aaagatatta ttaaagatta ttacgttggt agcattactg
    42841 taaatgcctt aaaaggaata aatttaaaca tttgcaagaa tgaatatgtt gcaataatgg
    42901 gtccttctgg aagcggaaaa tcaactttaa tgatgcacct gattgcttgc attacttccg
    42961 gcaaat
//
LOCUS       DAOPNX010000011        39888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_1325_length_39888_cov_6.518088, whole genome shotgun sequence.
ACCESSION   DAOPNX010000011 DAOPNX010000000
VERSION     DAOPNX010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 39888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39888
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1325_length_39888_cov_6.518088"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..1004
                     /locus_tag="PKN53_02235"
     CDS             93..1004
                     /locus_tag="PKN53_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOD87804.1"
                     /translation="MKPTLFILAAGMGSRYGGLKQLDGLGPNGETIMDYSVYDAIESG
                     FGKLVFVIRKSFEQDFREKIVKKYENKILIEIVFQELDYLPAGFTLNPERVKPWGTNH
                     AVMMGKNVISEPFAVINADDFYGRDAFRVLGKFLSELKNSENKYCMVAYQVGNTLSER
                     GTVARGICQSDKNNFLTGVVERTKVIRNDGKVQYLDENEKWVTIADDTPVSMNMWGFT
                     PDYFKHSDKLFIDFLNENSNNITAEFYIPRVVNDLIVNNISTVEVLKTTAKWFGVTYA
                     EDRSEVVEKLAQLVKKGEYPSPLWEIS"
     gene            complement(1074..1994)
                     /locus_tag="PKN53_02240"
     CDS             complement(1074..1994)
                     /locus_tag="PKN53_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87805.1"
                     /translation="MKRFILNTVLFLLFASIFYIVVLNLWGYYLPQSLKPNINYRIGS
                     YGHMYSRLSEVKNYGEVDILFLGSSHTYRGFDTRIFAEKGYKTFNLGSSSQTPIQTKV
                     LLHRYLDRLNPKLVIFEVYPEAFSLDGVEASLDIIANDKNDFQSIKMAFIINNVKTYN
                     TLIYGLTRELLGLNSPLIEDTIKRADKYVSGGFVEREMGFYQPEKIEEKEIVFQDYQL
                     KAFSEIVEKFEEKGIELILVYAPIPRPTYERYTNAKYFDSVMQGYATYYNFNEMISLN
                     DSLHFYDSHHLNQNGVEIFNEKIIELIESR"
     gene            complement(1998..3449)
                     /locus_tag="PKN53_02245"
     CDS             complement(1998..3449)
                     /locus_tag="PKN53_02245"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOD87806.1"
                     /translation="MLFNSIDFAIFLPIVFILYWFVINKNLKLQNAMLVVASYVFYGW
                     WDWRFLSLITFSIVVDYFMGLLLGKEENKKKRKLLLWVSIFVNLGFLGFFKYYNFFVD
                     NFVAAFSFFGQDIKANTLNIILPVGISFYTFQTLSYTIDVYRRKIEPTKDFIAFAAFV
                     SFFPQLVAGPIERATNLLPQFYKKRFFNYSKAVDGMRQILWGLFKKIIIADNCAEFAN
                     QIFNNSANMSGSMLVLGAVFFAFQIYGDFSGYSDIAIGTARLFGFNLMQNFNFPYFSR
                     DIAEFWRRWHISLSTWFRDYLYIPLGGSRGGTLMKVRNTFIIFIVSGFWHGANWTFII
                     WGALNAIYFLPLLLTKTNRTNLEIVAKGKIFPSLKETLFMLITFGLTVFAWIFFRAEN
                     IEHAISYISGIFSSSFFTIPKFEETARAITTMILVVIFILIEWSGREGQYAIEHIGEK
                     WYRPLRWIFYATIIFLIGMFMPTSESPFIYFQF"
     gene            complement(3600..5261)
                     /locus_tag="PKN53_02250"
     CDS             complement(3600..5261)
                     /locus_tag="PKN53_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HOD87807.1"
                     /translation="MKINFLNLLVIATLVVTMSACNSKKKDESKKEELTEMQKLVADY
                     AEVELTANLDHLTDGDKQAIVKLIEVAQIMDDLFWKDAIGDKEAFLNSIEDEDALKYA
                     LINYGPWDRLNGNKSFIEAYGEKPLGANYYPSDIAKEEFNAFDDPNKMSWYTLIRRDD
                     AGKLKCVWYYEEYAQEIEKAAQLLEEAAELVSDKEFQTYLRLRATALRTDDYLTSDLA
                     WMDMKNNKIDFVVGPIESYEDAFMGVKAAHSGQILIKDLEWSKNIEHFNEVLPQLQAG
                     LPVAPEYKTESATNSGDMNVYNAIYYGGDCNSGSKNIAINLPNDPRVHATKGSRKLQL
                     KNSMEAKFNTILLPIAELLIDETQLKHVKFEDAFFQNVMFHEVAHGLGVKYTINSKKS
                     VREALEEYYSPVEEAKADIAGLYMVTQLYEMGEFPEKDLMDNYVTFMAGIFRSVRFGV
                     ASSHGKANMMEFYYLKERGAFVKNEETGKYSVNFEKMKAAVAEMVNDIIVIQGDGNLE
                     AAKKWVDTQSNIGEELQKDLDKIAEAGIPKDIVFKQGLEVLGLKK"
     gene            complement(5285..6397)
                     /locus_tag="PKN53_02255"
     CDS             complement(5285..6397)
                     /locus_tag="PKN53_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87808.1"
                     /translation="MDEQNFNPELSETNQPDVVCKNCAAKLVFEPGTTSLACPYCGTV
                     NEIEIRDEEIEELDLEEHIQLAEEGSEIEEVSTVACEACGAVTTLDKNVVSSECPFCG
                     NKLIVKSESTTKIIKPKSLLPFKITVKEAGEHFKKWIRKRWFAPPGFKKRATQSNQMS
                     GVYLPYWTYDSDTYTEYRGQRGDDYQETETYTDGNGKTQTRTVTRTRWTSVSGSVDVF
                     FDDVLVLASKSLPDKNVTVLEPWDLENLTPFDEKFLSGFKAETYAIGLKDGFDIAKVK
                     MDPIIDSHIRSDIGGDRQRITYKNVTYSNITFKHTLLPIWISAYRFKNKVYRFIVNGR
                     TGKVQGERPWCWWKIALFILAIIAVIVLLAYLGENQ"
     gene            complement(6580..7458)
                     /gene="fabD"
                     /locus_tag="PKN53_02260"
     CDS             complement(6580..7458)
                     /gene="fabD"
                     /locus_tag="PKN53_02260"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOD87809.1"
                     /translation="MKAYVFPGQGSQYVGMGQELYNSNPKAKEMFEKANEILGFRITD
                     IIFNGTDEDLKQTKVTQPSIFLHSVITYMCLEEKPEPAMVAGHSLGEFSALVAAGALS
                     FEEGLKLVSIRAKAMQKACELNPSTMAAVLNLDFDIIDKICSEIDEVVVAANYNSPGQ
                     VVISGSNKGIEIAMEKMKEAGARRVLQLPVGGAFHSPLMLPAQEELSIAINQTNFKTP
                     VCPIYQNVDAKPYKNPEQIRENLIKQLTSPVRWTTTIQNMIADGAKHFIELGPGNVLQ
                     GLIAKIDRDVTTEGIK"
     gene            complement(7480..8094)
                     /gene="folE"
                     /locus_tag="PKN53_02265"
     CDS             complement(7480..8094)
                     /gene="folE"
                     /locus_tag="PKN53_02265"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOD87810.1"
                     /translation="MVESLNNSENGGYAKIEKYDDEKTRLLAQHYSEILKLLGEDAER
                     EGLLKTPKRVAKSMQFLTQGYNMNPEEILRSAMFREDYKQMVIVKDIELYSMCEHHLL
                     PFVGKAHVAYIPNHYITGLSKIARVVDVYARRLQVQERLTTQIKDCIQNTLQPLGVAV
                     VIEAQHLCMQMRGVQKQNSITTTSDFTGAFLKDATRQEFLSLIK"
     gene            8164..8811
                     /locus_tag="PKN53_02270"
     CDS             8164..8811
                     /locus_tag="PKN53_02270"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87811.1"
                     /translation="MKTLIITATSLEAKQIVDNLHFIKRSDTVFSNTNYNCDILTTGV
                     GLVATMFSMFTCVNIKEYDLLINLGTVGSFSENLKIGELVNVNSDSFEDICIRDNGKK
                     INVFNSKFNQHFRNLINNGRIYNTSDYPHFFHNLKKVSGTSVNIPEQNPISNYEIESM
                     EGAAFMLVCKHFKKNFIQIRGISNIILKTKRENWDFNTPINNYSDLTIDFLKQNK"
     gene            8808..9635
                     /locus_tag="PKN53_02275"
     CDS             8808..9635
                     /locus_tag="PKN53_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HOD87812.1"
                     /translation="MKLKLAISPCPNDTFMFDALINKKIDTKNFDFEIIIDDIEKLNT
                     YTKQQEVDISKTSFHNFLKISDNYQLLNSGTAMGKNCGPLIVSKRKIYPDELKDCKIA
                     IPGESTTANLLMQIFFNEAQNKNVYLFSDIEELVLSNECDAGLLIHETRFTYQKRGLK
                     LIADLGNLWENKFNLPIPLGGIIVKRSLPNKIKQEIDKMLSESINLAFKNLRGTISFM
                     KKHAVEMDEEIMMQHVNLYVNEYSKDIGDLGKKSIYKLREEFLKLNNLEDKSLDLFL"
     gene            complement(9682..10079)
                     /gene="ssrA"
                     /locus_tag="PKN53_02280"
     tmRNA           complement(9682..10079)
                     /gene="ssrA"
                     /locus_tag="PKN53_02280"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(10121..10771)
                     /gene="rpe"
                     /locus_tag="PKN53_02285"
     CDS             complement(10121..10771)
                     /gene="rpe"
                     /locus_tag="PKN53_02285"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004348612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOD87813.1"
                     /translation="MKNIILSPSMLSANFANLEKDIQMINESQADWFHLDIMDGVFVP
                     NISFGFPVVKYIKKFAQKPLDVHLMIVQPERYIAEWKKVGADILTVHYEACTHLHRTV
                     QEIKNAGMKAGVSLNPHTSVELLNDILPELDMVLIMSVNPGFGGQSFISQSLVKIKNL
                     REMADIYNPNLIIQVDGGVDTHNYKEIIKAGANSLVAGSAIFGSENPVETIKILKS"
     gene            complement(10833..11705)
                     /locus_tag="PKN53_02290"
     CDS             complement(10833..11705)
                     /locus_tag="PKN53_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOD87814.1"
                     /translation="MRQLRITRQVTNRDSIAFEKYLQEISREEMISSAEEIELAKRIK
                     DGDQEAVDKLVLANLRFVVSVAKQYQNQGLSLQDLVNEGNYGLLVAAKRFDETRGFKF
                     ISYAVWWIRQSILHAIAVKGRMIRLPLNKIGVLNKVYSTSLALEQKLEREPSLAEIAE
                     ETDYNFKNVNEVLKYQGKTLSMDAPLSNSEGDDDFTMGDLLSSTQTLSVEKTLNNDSL
                     KIEINEALKILSERERKIITMFFGLEDESPKTYEEISEALNLTRERIRQLKESSLKKL
                     RQSSKRNILQSYLI"
     gene            12068..13942
                     /gene="rho"
                     /locus_tag="PKN53_02295"
     CDS             12068..13942
                     /gene="rho"
                     /locus_tag="PKN53_02295"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOD87815.1"
                     /translation="MYDIIELNSKLLPELKEIAKDLGIHKVDAFRKSDLIYKILDVQA
                     IKAAEQEADKKKQSKVQNSDATNKASENGKRPRTKMKAQKITFSSPTPTQKNLFDEVQ
                     QVNNTLETKEKAVVEEQNKTKDTVDNKKEVEQKKSNAVAQNAQPNKNLAEAKPTNNAK
                     NTQTPHQKGQEKKMILKKPQTKLNDELTHLPDIETERPIQAESTKVEPAKVETTKVET
                     TKVESTEVVKTNGETKQKEQEPKKPKKEDLYDFSGIITVTGVLEVIPEGYGFLRSSDY
                     HYLNSPDDVYVSQSQIKLFGLRTGDTVEGSIRPPKEGEKYFPLTKVIKINGIDPALMR
                     DRVPFDHLTPLFPDEKFNITGNGKATLSTRIVDLFAPIGKGQRGLIVAQPKTGKTVLL
                     KEIANAIAANHPEVYLMILLIDERPEEVTDMARNVNAEVIASTFDEPAERHVRVSSIV
                     LEKAKRMVECGHDVVILLDSITRLARAYNTVAPASGRVLSGGVDSNALHKPKRFFGAA
                     RNIENGGSLTIIATALTETGSKMDEVIFEEFKGTGNMELQLDRKLSNRRIYPAVDITA
                     SSTRREDLLIDNELINRLWILREFLADMNSVEAMEFMRTRLMQTATNEEFLWTMDKGL
                     "
     gene            14392..15951
                     /locus_tag="PKN53_02300"
     CDS             14392..15951
                     /locus_tag="PKN53_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HOD87816.1"
                     /translation="MKTSKLGLDQKKIESARQIAKNIADEVQDFVNQYTTVAVERSLC
                     RLLGIDGVNSNDIPLPNVVIDQIQENKSLSEGILFFLGNAILETGLNPQQIAEKIANE
                     DFNICKLPIRDKASIEKAIMPYVNASVEKIKKNVAIRNEYLSRLGEGSKPYIYVIVAT
                     GNIYEDAIQAQAAARQGADIIAVIRTTGQSLLDYVPYGATTEGFGGTFATQENFKIMR
                     AALDEVGEEVGRYIRLCNYCSGLCMPEIAAMGAIERLDVMLNDALYGILFRDINQQRT
                     IVDQYFSRVINGFAGVIINTGEDNYLTTADAYEQAHTVLASDLINEQLALLAGMPEEQ
                     MGLGHAFEMDPEMENGFLYELAQAQMTREIFPKAPLKYMPPTKFMTGNIFKGHIQNAL
                     FNVVSIWTQQGIQLLGMLTEAIHTPFISDRYLAIENSKYVFNNLKNIGDEVEFKENGI
                     IQKRAQEVLNNAHALLSEIDKVGLFDALEKGLFADTKRSKTGGKGLAGVVQKSENYFN
                     PFIEIMKGGNK"
     gene            15951..16742
                     /locus_tag="PKN53_02305"
     CDS             15951..16742
                     /locus_tag="PKN53_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HOD87817.1"
                     /translation="MSGGNYSMDKKEFDKILDLKKVKPYGDTMNDGKVQLSFTLPVPC
                     SDEASEAAKQLMRKMGFEEPQVVYFKELTKGFTFFVCYGSCTHTVDFTSIHVPKVESN
                     VMNMAETDEFISQNIGRKLVVIGASTGTDAHTVGIDAIMNMKGYAGHFGLERYEMIEA
                     LNMGAQVLNEDFIAKAIELKADALLVSQTVTQKDIHIKNLTELVEMLEAEGLRDKVLL
                     ICGGPRISHELAKELGYDAGFGMNSYADDVASYIAQEMDRRINNK"
     gene            16754..17416
                     /locus_tag="PKN53_02310"
     CDS             16754..17416
                     /locus_tag="PKN53_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HOD87818.1"
                     /translation="MDKNQMREVIAKRVAKEINDGDIVNLGIGIPTLVPNYLPEGVKV
                     TLQSENGLLGMGPSPEPGKEDENLVNAGGGYITANLGAASFDSSRSFGIVRGGHVDVT
                     VLGAMQVDPSGDLANWTIPGKLTPGMGGAMDLLVGARRVILAMEHTAKGRPKILKKCT
                     LPLTAKGQVNTIITEMCVMEITEKGIVLTEIHPEFTVEDIKAVTEADFIVSENLKEMQ
                     LD"
     gene            17454..18659
                     /locus_tag="PKN53_02315"
     CDS             17454..18659
                     /locus_tag="PKN53_02315"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOD87819.1"
                     /translation="MKKVFLIEAKRTAIGKFGGSLATVSASDMAAEVIKTIISNTGIN
                     PADLDEVITGNVLSAGQRQGVSRQAAIKAGIPNEVPAYSLNMVCGSGMKAVSNAVSAI
                     RAGEANLILAGGTETMSGAPMLLPASVRTGTKMGDFKVIDHMILDGLWEAFNDYHMGN
                     TAENIAEKYNISREEQDQFAFASQTKAAKSQDAGNFKKEIIPINIVTRKETIVFDQDE
                     FINRTTDLEKLAKLRPAFKKDGTVTAGNASGINDGASYVLVASEEAVEKFKLKPMAEI
                     VAVGQGGVDPAIMGMGPVPAINNVLKKANMKLQDIEVLELNEAFAAQSLGVIKQLCTD
                     HGVDKSFFDERCNKNGGAIALGHPIGASGNRIIVSLIHEMKRSNNKFGLASLCIGGGM
                     GTAIILKNI"
     gene            18779..19801
                     /locus_tag="PKN53_02320"
     CDS             18779..19801
                     /locus_tag="PKN53_02320"
                     /inference="COORDINATES: protein motif:HMM:NF027446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4836 family protein"
                     /protein_id="HOD87820.1"
                     /translation="MKKIRLSSTIIVLVLVAVFFVSCSKNNSSHIPKNAFVVAVIDGK
                     GLKDLTNNNFLKDNEEYKQTMESLKQESEKLVELIEKTQKNPDELGILLTKRSYAFAS
                     YDKEELLYGFIIPINKKKLEANLDMIGSELGFPISAVLSTKDEIKYFADSDMILGWNK
                     DVFIFLGKESGEVDFELLEKHFNLKKDESILANDDFNKFHKNCKDLNLWISSDIIKNV
                     EEFDSEIKEFETLTGIDLANNYGHMHLEFAKGEINFTSSLRYNKSIQELDMEKLTENS
                     ETLEKFLSKYTDFINICGLNNKYAEEYDYDYDYEYDEDINWEDYQSEFTEEEWEEFLK
                     ALEEVE"
     gene            19820..20458
                     /locus_tag="PKN53_02325"
     CDS             19820..20458
                     /locus_tag="PKN53_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD87821.1"
                     /translation="MEIRFSKIIPIPIPESNIANSEIWNRDLLFEQNNKYLIYADSGK
                     GKTTFVNIIFGLRKDYTGNVYIDGEDISNFSINQFSQLRKTRLSIVPQGLQLFPELSV
                     LENINIKNRIQNYKTSKQISEMIEKLGLSGFENRKTAKMSFGQKQRVAIIRALCQSFD
                     YILLDEPFSHLDAKNTEIAWDLIKSEAEQQNASIIVTSLKQDFETNIIKLRV"
     gene            20458..21669
                     /locus_tag="PKN53_02330"
     CDS             20458..21669
                     /locus_tag="PKN53_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87822.1"
                     /translation="MIWKVLKNNIKPAQLIGTFFGITLGLSILMVAVSFYLDVKPIFE
                     DKESFWRDEYIIVNKKVKITDSYHQIREADKEKPYFTEEEIEDLKRQAFVKDVAEFSN
                     CSFAISASADRDSPLSGFYTDLFFEAVPDNYVDVNYKNWNWTENSAYIPVILPKVYLN
                     LYNFGFAQSQNLPQISESGASLAKFNIHVYGQKKHQKFEARIVGFSERINTILAPKSF
                     VDWGNKNFGEHEKSKPGRIIIIAKDPSSQELVEYIDNHDYDINKSELSNSKALAFLKI
                     IIGIVVTIGIIIICLTFALIILSIQLLLQRNNENIVKLNMLGYSLKEISLPYKIMVSI
                     LFAASFISSLIPLYFLRNYYFSNLVLFGYESTTQLFSNIILPSLIFIFIVVLLLISML
                     KSTIKKIISKN"
     gene            21852..23162
                     /locus_tag="PKN53_02335"
     CDS             21852..23162
                     /locus_tag="PKN53_02335"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOD87823.1"
                     /translation="MKISIIGTGYVGLVSGTCFAEMGITTSCLDVDQQKIENLKNGIM
                     PIWEPGLKEMVKRNFEKQRLSFTTNISESINSASACFIAVGTPPDEDGSADLQYVLAV
                     AKEIGQNINNYMVVIIKSTVPVGTSEKVRATIQKEIEKRGVNIKFDIASNPEFLKEGD
                     AIEDCLHPDRIIVGTDSEKAREIINRIYSPFVLNGHPIIHMDIASAELTKYTANAMLA
                     TKISFINDIANLCEIVGADINAVRKGIGTDKRIGPYFIYAGTGYGGSCFPKDVKAIIK
                     TADEHGHSLEILKSVENVNNRQKEILFHKANKHFEGNLKNKKFAIWGLSFKPNTDDMR
                     EAPSIILINNLWEAGADIYAYDPVAMKEAKKILPKKVKYCKTPYEAVEEADALFLVTE
                     WTEFRVLNYKALDKMKNKVIFDGRNIYQPEELNKNGFTYYGIGR"
     gene            23170..23424
                     /locus_tag="PKN53_02340"
     CDS             23170..23424
                     /locus_tag="PKN53_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOD87824.1"
                     /translation="MSKIEVTICLGSSCFSRKKRDIVSALQDYIDQKNLSNRINLKGG
                     HCLGKCSVGPIIVVNEQIYTGITPAKAIRIIEEYLSTQNN"
     gene            23426..25276
                     /locus_tag="PKN53_02345"
     CDS             23426..25276
                     /locus_tag="PKN53_02345"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOD87825.1"
                     /translation="MQLITFDKEKCLSCYACVKACPVKAITISSSEEILSFNHNRCLG
                     CGNCYISCGPKAIGYIDSTEAVSQLLNSETPCIAIVDPSISAEFPDILDVRNFVGMIR
                     ALGFEFVNEISFGADVIAYKYKNLFDNFKGKYHITTNCPPIYDYVEKFQTHIIDSLVK
                     IDSPMIATAKIMKEIHGDDCKIIAITPCIAQKREAKRFGFVDEVISFEELRILFNQNK
                     IYENSVEFSEFDMPLGAKGSLYPLSTGILNAADINYTDIDSKIITKDGAEHFTQFMND
                     FASISLIKHHLNIFYCHGCAMGPGSKNNNSYLRNHSLVVDYTKRRLNSAVDKQEWEKN
                     LEKFSKLDFTRSFTANDQRIPTPSQAKIKEVIDLLGKDQLYEVGCRSCGYKNCSEFVT
                     DVAKGLARPEMCQNFSKTNKQNYINKINIANANLEETKKNLEELEIKLQKEHKDVKVM
                     ATLTKAIIEKIPSAVIVVDDKLKILQSNQKFIELLGQDAKEIDEVVPGLIGADIKTLL
                     PSQIYNFFSFVLNKDETIDNKDVEIDDKLITVSIFPIEKGKTIGAVFKNLYQSEVRKE
                     EVMYRINDVIEKNLGMVQQIGFLLGEGASETERMLNSIINSFEKEDKEEK"
     gene            25283..26467
                     /locus_tag="PKN53_02350"
     CDS             25283..26467
                     /locus_tag="PKN53_02350"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOD87826.1"
                     /translation="MSEKLYIEVDVRQINYHGERICGDVFLKKRVKEDNRIIAVLSDG
                     MGHGVKANILATLTSTMALNFTIEHKKPERIAEIIMNTLPVCSERQVSYATFTVVDIE
                     FDGQVHILEYDNPALLVFRKNKLLKLPYETIEMKNPPHAGKKLTYTSFYPKLHDRLVF
                     CSDGVSQSGMGTDKYPFGWGVLGIEKHVTGILEITKDISASKLAEKVVNKAVANDIYI
                     PKDDTSCAVVYFRKPRKLVIATGPPTYRESDKELAEFIKNYDGKKIVAGGSTTDLIAR
                     ELDLKVSTSLEFADPELPPISHIEGIDLVTEGILTLNKVWKILTEYEPQYKLGRGPAD
                     QIVKLILESDHIDIVVGTKINEAHQDPNVPIDLEIRRTIANRIANILENKFLKHITIK
                     YF"
     gene            26574..27158
                     /gene="ruvA"
                     /locus_tag="PKN53_02355"
     CDS             26574..27158
                     /gene="ruvA"
                     /locus_tag="PKN53_02355"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOD87827.1"
                     /translation="MFEYIKGLLTELSPTYAIIEANGIGYFINITVNTYSKLKKGQEC
                     KLFLHEILREDLHAIYGFENLSEREMFRKLITVNGVGANTARLILSSLSVNNVVQAIN
                     EENINVLKSVKGIGVKTAQRIIIELKDKLSDSDIQISEISSSLNNTLQKDALNALTML
                     GFSKPNVEKVLIKLLKDNKEISLENLIKLALNYL"
     gene            27168..34619
                     /gene="sprA"
                     /locus_tag="PKN53_02360"
     CDS             27168..34619
                     /gene="sprA"
                     /locus_tag="PKN53_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOD87828.1"
                     /translation="MITKIFKHTFLFSLIIGIFSIIFASNAKLNYNIPKRNPILIDNF
                     DDDTIKGAKLNYPIPEEDINPANNKDKSPLYGSDPSNIETEVVYDAESNQYIFQKKIG
                     GENFGTPYSMSFDEYLDYDFEKAMKSYWRQRSKDDISETRDVLIPKLQVDGEIFDRIF
                     GGSNIDIRPQGSAELIFGLKVDKIENPAMPLHAQSPVTFDFQEKIQMNVVGQIGEKMK
                     VNLQYDTESAFDFENSVKLEYTGHEDEIIQKIEAGNVSLPLTGTLITGGQSLFGLKTE
                     AKFGKLTVTTLFSQKKGEMSTVRVEGGAQSQDFEIKADEYEANKHYFLSHYFKENYDR
                     ALAQLPIINSGINITRIEVWVTNKAGNFENARNIVAFADLGESNDNNIQSDYVRTANG
                     NTTTVYPHSEINILGEITTTYPDVRNINNVGNTLLGLGMTGGRDYEKIESARLLSPNE
                     YTVNERLGYISLNATLNPDQVLAVAYEYTVAGNVFKVGEFSNSAITAPDALVVKLIKG
                     TAFTPQLKSWSLMMKNIYNIGAYGITNEDFWLDIMYTNDKTGTEINYIPAGAIDSIRL
                     LEVLNLDNLNSQHDPYPDGIFDFIEGYTVSTSNGRMMFPVTEPFGSYLKKKITGGDAS
                     LNNIAETYIFQELYDSTQNTAGQIAEKNKFRIKGKFKSAGGSEIRLNVANIPQGAVIV
                     TAGAVKLVENVDYTVNYNLSTVKIINQGILDSKTPIDISLESNSGFNMQTKTLIGSHV
                     NYEFSKDFNIGATILNLTEKPLQTKVGIGYEPISNTIWGFNTSYRTDVPFLTKMIDFI
                     PLIETKEMSTLTFTGEFAHLIPGHSRAIKKEGNAYIDDFEGTKTSLDLKSQSAWTLAS
                     TPADVSMFPESVLTNDLRYGFNRAKLNWFTIEPMLLKPNNSPVSIEDQSSHYVREVYE
                     KELFPNRESQSSIPVHLYTLNLAYYPHKKGPYNFDVDGTNADGSLKYPDTRWGGIMRP
                     IQQPDFERDNIEYIEFWLMDPFVEDSTNVGGDLFFNLGDVSEDVLKDGRKSFEQGLPT
                     PSTDNPVDTTAWGLVPLMQALVPAFDNDAESRVAQDVGYDGLSTEKEREFFKAYLDSL
                     AAKHGVNSPAYIKAYNDPSNDDYHHFRGSDYDSDNLGILERYKLYNNPEGNSPPAEFS
                     QENYSTSGKTDPNVEDINRDNTLNEGENYFQYRVSIRPQDFEVGKNYITDKVIVSSTF
                     VNDKQSKVAWYQFKIPIHSYDKRIGNIQDFKSIRFMRMFMTNFTADQTILRFATLDLV
                     RGEWRKYNASFMQPGEYIVDELADTPFEVSAVNIEENSNKVPVNYVLPPGINRESDPM
                     NPQHRQLNEQSMSLKVCGLQDGDARAVYKNVNLDVRRYLRLKMFIHAESVPGEQPIQD
                     NDISVFVRLGTDYKNNYYEYEIPLKVTPNGRYDGTLDEQAPDRYIVWPEENDLNLDFD
                     LLQQVKQSRNDKIRNGDPNVSITRLYSMVDGNNKISIMGNPNLSNVQTIMIGIRNPKK
                     RSVNDTDDGQPKCAEVWVNELRLTNFDERGGWAANARLTAKLADLGSVTVAGSTSKPG
                     FGSINQKIGERQKEEINRYDISSNFELGKFFPEKLNVRIPMYIGYSENVKNPEFNPLD
                     PDIPFKVALSDPNLTKEYKDSIKHISQDYSMRKSINFSNVKVNKTSGKSNIYDLANWS
                     LSYGYNETYQRDVNTIYNTTKLYQGAINYNYNATPKIVEPFKNVKLFNKKAFQLIRDF
                     NFYYLPSQLSFRTNINRSYGETQLRNVYNSSLALPVNVRKDFTWIRQYDFKFNITKNL
                     KLDFTATNNARIDEPQGRIYKDDPFYEEKRDTIWNNFKNFGRNTQYHHQWDLTYNLPI
                     NKIPLLNWVTANTRYSGSYDWITSPITADTIKLGNTLQNGNTMAVNTQFNLLGLYNKI
                     KYLDQVNKKYRGRQSSRPKKKEVENVTFEQTNVNLKADKAKKINHKLKTEDVNIKVIA
                     PDGVEIVGEMKIIDANRIEFTPKNDVKDATLTVTGKREKKESIPKIIFDNTLVVLMSV
                     KNISIAYTETNGTLLPGYMPNTYIMGMSKYMADPEMFGPTPSILTPTIPFIFGWQEDG
                     FSDWALTNNVITKDTTNLQAFMLTNNRNWNVRASIEPFRDLRIDLNLTHSFSQNRSNF
                     FRYGYDASQNADAYMTLNPSQMGSFSMSTITWGSAFEPLKKGGDYSSKVFSQFSENRI
                     IIAHRLASQRPGYNADDIDTYGFPSGYGKTSQDVLIPAFLAAYTNKDAKEVGLETFPS
                     ILKALPNWRITYDGLGKIPALRKIFRTVNITHNYRSTFNIGSYKTRLENEYYESDGFN
                     IIRDEIANYYSKYEINGISISEQFAPLLSVDVTMINSFIVKLELKKTRNLNMSFSNNQ
                     LTDMRNNEYIIGTGYRFKDVEINIKSGGRTRNFKSDLNVRLDFSLRDNLTVTRKLEEG
                     FNQPTAGTQTYTIKASADYVLSNRFNLRLFYDQVINRPTMGVSFKSSNINFGVSLRFT
                     LAT"
     gene            34674..35054
                     /gene="gcvH"
                     /locus_tag="PKN53_02365"
     CDS             34674..35054
                     /gene="gcvH"
                     /locus_tag="PKN53_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOD87829.1"
                     /translation="MNIPKNLKYAESHEWVRVEGDVAVVGITDFAQTSLGDIVFLDIE
                     VEGETLDKGEVYGSIEAVKTVSDSYMPVSGEVIEVNQEAIDDPSIVNKDPYGEGWLLK
                     IKMSDPSEVNSLLDAAGYEEIAKD"
     gene            35198..35899
                     /gene="ispD"
                     /locus_tag="PKN53_02370"
     CDS             35198..35899
                     /gene="ispD"
                     /locus_tag="PKN53_02370"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF001183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOD87830.1"
                     /translation="MVYAIILSGGIGTRFGNQLPKQFLKINNKTILEYSIETFETHNL
                     INKIIIVSNPNFVEETKEILAKNNYKKVCAIISGGNTRGESAYRGLKQINETENVKDK
                     IKVLIHDSVRPNTSNKIISEIIKGFDEFKALSPAIKTTDTIYISNATQIIKSIPNRDN
                     IYKAQTPQGFDFNTIFNAYNKLDDSQKFAFPDDCSVLNFAYPNIEIKIVEGDNKNLKI
                     TFPEDLEVFKQLLTQ"
     gene            36033..36200
                     /locus_tag="PKN53_02375"
     CDS             36033..36200
                     /locus_tag="PKN53_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87831.1"
                     /translation="MKKSVLKIVYLLIVLINSALYVNAQCTTNAGNMNQTEPVYLCDN
                     QCSEVGHSGAL"
     gene            36386..38008
                     /locus_tag="PKN53_02380"
     CDS             36386..38008
                     /locus_tag="PKN53_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87832.1"
                     /translation="MHQSNPFAITWFSTPTAQISQTQTSFCGLTGFLNATPPQSGLSG
                     YWSSNVEFVPINGGNVNDANTDIMVSQPGTHTFTWTVTNGTCSDVASVEITFVQAPNP
                     IVTNAYDTVCGNSYNLNVITTPDADGQWSSYIWNDEENNWVIPTTPPYISLVTSPNTT
                     VNIANYPGATCRYRFEWTETNTTGGIICQGTASKEVVFAKTPMASVGLVSEAELCGNS
                     FQLDADTSGYSWATGKWISSNIANPFDDPNLPNATVTISNPENFGDSAYVQFPFVWTM
                     TNTICITTDTMWVTFYQRPQANAELDDAVCGYEYNLHAQWSLAPSPNYTPSGVWQMVP
                     QPSTATADIQDIYNNNSHVTVSETGVYQFVFRENNTNLTSCYTTDTVQIEFVENPIAY
                     AGEDQDVCGYCATLGAISGGFPGAWLPNGTSYEDHTDPTTQVCTSYYGTIVYTWKASN
                     TAITNNSFTCVATDDVSITFWKKPTPNILTDPADSTACGLTYYKLRAEFPGIGITGYW
                     YCSTTGVVFENSNYTETSVTVPHYGYYDFYWI"
     gene            38075..39673
                     /locus_tag="PKN53_02385"
     CDS             38075..39673
                     /locus_tag="PKN53_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD87833.1"
                     /translation="MEAPLANAGGDTYFCGLTGQLNAIPSIGIGTWSTSSTEYINFED
                     TNDPNTTITSTIINTENSDQPYFEVIWTENYTNGCTDADTIKVVFATASNSYSEEHSI
                     CEGDIYSWNEQDYTETGTYTAEFTNVYGCDSIITLNLTVNPLPEQIVVSQNPENGILS
                     EGTSGSITLENSIIETNYWVSMGGEPFTEEITGTGSELSLGNNFTDGSYDIRSRNQFD
                     CSLVQGIANFVTEVSGTNKIVANVSFGTPASNFPANHVNVKLYKATTNNETIVLVAEQ
                     LLSSNGQVEFTNLETGDYYLGSFIQYPENYNVAEHVYYQNVVVHENATSIPMAEETVY
                     IANIHHIALAESQGSNVMYGIVGTMNSNESLNPLNEMVVVLRNSDINEFIGVKVTGDN
                     GQYHFGNIPESTNIQAFVTSFIHPNWTAFEIETTNDQNYNVNFIVEGTSVYPDYITML
                     EDILIQNIEFTIFPNPAKEVLYISCDIEKAVYQIFDINGRLVNSGLAFSNSEINISDL
                     VSGTYLIILSTDNGKTGIQKFVKQ"
BASE COUNT        14646 a         6158 c         6419 g        12665 t
ORIGIN      
        1 cgtggttgct gaacgaagtt gaagtaaagc agtttgacac ttatgagaca ccgccgtttt
       61 ttattatttt aaaaacacaa aaattaatat ttatgaaacc tacacttttt attttagcag
      121 caggaatggg aagccgttac ggtggtttaa aacaacttga tggacttggt cccaacggcg
      181 aaacaataat ggactattct gtttatgatg ccatagaatc aggatttgga aaacttgttt
      241 ttgttattcg taaatctttt gagcaagatt ttagagaaaa aattgtaaaa aaatacgaaa
      301 ataaaattct aatagaaatt gttttccaag aattagatta tttgcctgct ggctttactc
      361 taaatcctga aagagtaaaa ccttggggaa ctaaccatgc tgtgatgatg ggtaaaaatg
      421 taatttctga acctttcgcc gtaataaatg cagacgattt ttatggtaga gatgctttta
      481 gagttttagg caaattttta tcagaattaa aaaactctga aaataaatat tgcatggtcg
      541 cctatcaagt tggaaacacg ctatcagaac gtggaactgt agcacgtgga atttgccaaa
      601 gcgacaaaaa caattttctt actggcgtag tggaaagaac aaaagttata agaaatgacg
      661 gaaaagtgca atatcttgat gagaatgaaa aatgggttac aatagcagac gacactcccg
      721 tatcaatgaa tatgtggggt ttcacaccag attattttaa acattccgat aaacttttta
      781 ttgatttctt aaacgaaaat tctaataata ttacagctga attttatatt cctagagtgg
      841 taaatgattt aatcgtgaat aatatttcta ccgtggaagt tttaaaaact acagcaaaat
      901 ggtttggagt tacttacgcc gaagacagaa gcgaagttgt tgaaaaatta gctcaattag
      961 taaaaaaagg cgaatatcca agcccgttat gggaaatcag ttaaaagttt ataaagttcg
     1021 taaagtctga agattaaaag atttgaagat attttggctt gagattttta tattcatcta
     1081 ctttctatta attctataat tttttcatta aaaatctcaa ctccattttg attcaaatga
     1141 tgagaatcat aaaagtgcaa tgaatcattc agggaaatca tttcattaaa attgtaatat
     1201 gttgcatatc cttgcatcac actatcaaaa tatttggcat tggtatatct ttcataagta
     1261 ggtcttggaa ttggagcata tactaaaatc aattctatgc ctttttcttc aaatttttca
     1321 acaatttcag aaaaagcttt aagctgataa tcttgaaaca caatttcttt ttcttcaatt
     1381 ttttcaggtt ggtaaaagcc catttctctc tcgacaaaac caccagacac atatttatct
     1441 gcacgtttaa ttgtatcttc aataagagga gaatttaatc ctaacaattc acgagttaat
     1501 ccataaataa gagtgttata ggtttttaca ttattgatta taaaagccat ttttattgat
     1561 tgaaaatcat ttttatcatt agcaattata tcaagagatg cctcaacacc gtctaaagaa
     1621 aatgcctctg gataaacttc aaaaattact agttttggat ttaatctatc taaatatcta
     1681 tgcaacaaaa ctttagtttg aattggtgtt tgagaactag aacctaaatt aaatgttttg
     1741 tatccttttt ctgcaaaaat tcttgtatca aagcctcggt aagtgtgaga tgaacccaaa
     1801 aacaaaatat caacttcacc ataatttttt acttcagaca atctagaata catatgccca
     1861 tatgagccta ttctataatt aatatttggt tttaaggatt gaggcaaata atatccccat
     1921 aagttaagaa ctacaatata aaatattgaa gcaaataata gaaacagaac cgtatttaga
     1981 ataaatcttt tcatagctta gaattgaaaa taaataaatg gtgattcgct agttggcata
     2041 aacattccta ttaaaaaaat tattgttgca tagaatatcc atcttagtgg tctataccat
     2101 ttttctccaa tatgttcaat tgcatattgt ccttctcttc cactccattc aattaaaata
     2161 aaaattacaa ctagtatcat tgttgtgata gctcttgctg tttcctcaaa ttttggtata
     2221 gtaaaaaaag aagaagaaaa tattccagaa atatagctta tagcatgttc gatattttct
     2281 gctctgaaaa atatccaagc gaaaactgtc aatccaaatg ttattaacat aaacaaagtt
     2341 tcttttaaac ttgggaatat ttttcctttt gctacaattt ctaaattggt tctattggtt
     2401 tttgttaata aaagtggtaa aaagtatatt gcattcaacg caccccaaat aataaatgtc
     2461 caatttgcac catgccaaaa tccactgaca ataaaaataa taaaagtatt tctaactttc
     2521 atcaaagttc cgcctctact tccacctaat ggtatataaa gataatctct aaaccatgtt
     2581 gaaagagaga tatgccaacg tctccaaaac tctgcaatat ctcttgagaa atatggaaag
     2641 ttaaagtttt gcattaaatt aaatccaaaa agtcttgctg taccaattgc aatatcagaa
     2701 tagccagaga agtcgccata aatttgaaaa gcaaaaaaca cagctcctaa aacaagcata
     2761 cttccgctca tattggcaga attattaaag atttgatttg caaactcagc acaattatct
     2821 gcaataataa tttttttaaa caaaccccaa agtatttgtc gcattccatc aactgctttt
     2881 gagtaattaa aaaatcgctt tttataaaac tgtggaagta aattagttgc tctctcaata
     2941 ggtccagcaa ctaattgagg aaaaaagcta acaaatgcag caaaggcaat aaaatctttt
     3001 gttggttcaa tttttcgtct gtaaacatca atggtataac tcaaagtttg aaaagtgtaa
     3061 aaacttattc ctactggtaa aatgatattt aaagtattgg ctttaatatc ttgtccaaaa
     3121 aatgagaaag ctgcaacaaa gttatctaca aaaaaattat aatatttaaa gaagcctaaa
     3181 aatccaagat ttacaaaaat actcacccac aataaaagtt ttcgtttctt tttattttcc
     3241 tcttttccca aaagaagccc cataaaataa tcaacaacaa tactgaatgt tatcaatgat
     3301 aaaaaacgcc aatcccacca gccatagaac acatagcttg ctacaacaag catagcgttt
     3361 tgtaatttta agtttttatt tattacaaac caataaagta taaaaactat cggtaaaaaa
     3421 atcgcaaaat caattgagtt aaaaagcatt gtgttctttt atcttttaat tttagaaact
     3481 cattttgtgc ttgcaaaagt aaataaaaat atctaatatt ttataaaagg aacttttaaa
     3541 acaaaaaagc cttgcaatat attttatttg caaggctttt caataatttg tattagcaat
     3601 tatttcttca atcctaaaac ttctaatcct tgtttaaaca ctatatcttt tggaatacca
     3661 gcttcagcaa ttttgtcgag gtctttttga agttcttcgc ctatattgct ttgtgtatcg
     3721 acccactttt tagctgcttc aagatttcca tcaccttgaa taacaataat atcatttacc
     3781 atttcagcaa ccgcagcttt cattttttca aaattaacag aatatttgcc tgtttcttca
     3841 tttttaacaa aagctcctct ttcttttaaa tagtaaaatt ccatcatatt agcttttcca
     3901 tgtgaacttg ctacgccaaa acgtacagaa cggaaaattc cagccataaa tgtaacataa
     3961 ttatccatta aatctttttc tggaaattcg cccatttcgt aaagttgagt aaccatgtat
     4021 aagcctgcaa tatctgcttt tgcttcttct actgggctat aatattcttc taaagcttca
     4081 cgaacagact ttttagaatt gatagtatat tttactccta aaccatgagc aacttcgtga
     4141 aacattacat tttggaaaaa tgcatcttcg aattttacat gttttaattg agtttcatca
     4201 attaacaact cagctattgg aagtaaaatt gtattaaatt tagcttccat agaatttttc
     4261 aattgtagtt ttctagatcc tttagttgcg tgaactcttg ggtcgttagg taaatttatt
     4321 gcaatatttt tacttcctga attacaatcg ccaccataat aaatagcatt ataaacattc
     4381 atatcgccag aatttgttgc agattctgtt ttgtattctg gagcaactgg caatcctgct
     4441 tgaagttgtg gtagaacttc gttgaaatgc tctatgtttt tactccattc taaatctttt
     4501 attaaaattt gtcctgaatg agctgctttt actcccataa atgcatcttc gtaactttcg
     4561 ataggaccaa caacaaaatc aattttattg tttttcatat ccatccaagc taaatcactt
     4621 gtaagataat catcagtacg taaagctgtt gctcttagac gtaaataagt ttgaaattct
     4681 ttatcgctaa ctaattctgc cgcctcttct aagagttgag ctgctttttc aatttcttga
     4741 gcatattctt cgtaatacca tacacatttt aacttaccag catcgtctct acgtattaaa
     4801 gtgtaccagc tcattttgtt tgggtcgtca aatgcattaa attcttcttt tgcaatatcg
     4861 cttggataat aatttgcacc caaaggtttt tcaccataag cttcaataaa agatttgttt
     4921 ccgtttaatc tatcccaagg accataatta attaatgcgt acttcaacgc atcttcatct
     4981 tctatagaat ttaaaaaagc ttctttgtcg ccaattgcat ctttccagaa taaatcatcc
     5041 ataatttggg caacttctat aagttttact atagcttgtt tatcaccatc ggttaaatgg
     5101 tcgagatttg cagtaagttc aacttctgca taatcggcta ctaatttttg catttctgtt
     5161 aattcttctt tttttgattc atcttttttc ttactattac atgcactcat tgttacaaca
     5221 agtgttgcaa taactaataa atttaaaaaa ttgattttca taattataaa attaaattaa
     5281 aaaattattg attttctcct aaatatgcta atagtactat tactgctata attgcaagga
     5341 taaataaagc aattttccac cagcaccaag gtctttcgcc ttgtacttta cctgttctac
     5401 cattaacaat aaagcgatat actttatttt taaatcgata tgcactaatc caaataggca
     5461 ataaagtatg tttaaaagtt atatttgagt aagtaacatt tttataagta attctttgtc
     5521 tgtctccacc tatatcgcta cgaatatgac tgtctataat tgggtccatt tttacttttg
     5581 cgatatcaaa tccatctttc agtcctatag cataagtttc agctttaaat cccgacaaga
     5641 atttttcgtc aaatggtgtt agattttcca aatcccaagg ttctaaaacg gttacatttt
     5701 tgtcgggcaa agatttactt gcaagcacta acacatcatc aaaaaataca tctacactac
     5761 ccgaaacgct tgtccagcga gtcctagtaa ctgttctagt ttgagtttta ccattaccat
     5821 cagtatatgt ttcagtttct tgataatcat ctcctctttg acctctatac tctgtataag
     5881 tatctgaatc gtaagtccaa taaggtaaat acactcctga catttgattt gattgagtag
     5941 cacgtttttt aaatcctgga ggtgcaaacc agcgttttct tatccatttt ttaaaatgct
     6001 ctcctgcttc ttttacggta attttaaaag gaagcaaaga tttaggctta attatttttg
     6061 ttgtactttc actttttaca attaatttat tcccacaaaa tgggcactcg cttgatacca
     6121 catttttatc taaagtagta acagctccac aagcttcaca agctactgtt gatacttctt
     6181 ctatttccga accttcttct gcaagctgta tatgttcttc taaatcaagt tcttcaattt
     6241 cctcatctct aatttcaatt tcgttaacag taccacagta aggacaagct aaagaagttg
     6301 ttccaggctc aaaaaccaat tttgcggcac agtttttaca aactacatct ggctgatttg
     6361 tttcagacag ttcaggatta aaattttgtt cgtccattgc taaatttttt taaagtgttt
     6421 tcaggcgtaa aattaagatt tttttttaat taaaacactt tttttgtttt tattcaacaa
     6481 aggattttga ttttataaaa ttaacaaaaa gtcaagcgta aaaaaatcgt tctaaaacaa
     6541 agaaattgca acctatctta aaatacaaag acacctcttt tattttattc cttctgtagt
     6601 aacgtctcta tcaatttttg cgattagtcc ttgcaacaca tttccaggtc caagttctat
     6661 aaaatgttta gctccatctg caatcatatt ttgaattgta gttgtccatc ttactgggct
     6721 agtgagttgt ttgattaaat tttctcggat ttgttctgga tttttgtatg gttttgcatc
     6781 tacattttga taaattgggc aaacaggagt tttaaaattt gtttgattga tagcaatact
     6841 aagttcttct tgagctggca acattaaagg agaatgaaaa gcacctccaa ctggtaactg
     6901 tagaactcgt cttgctccag cttctttcat tttttccata gcaatttcaa ttcctttatt
     6961 tgagcccgaa atcacaactt gtccaggcga attgtaatta gcagcaacta caacctcatc
     7021 tatttcggaa caaattttat cgataatatc aaaatctaaa tttaatactg ctgccatagt
     7081 agaaggattt aactcacatg ctttttgcat agctttagca cgaattgaaa ctaacttcaa
     7141 gccttcttca aaactaagag cgccagcagc aaccaaagcc gaaaattcac caagcgaatg
     7201 tccggcaacc attgctggtt caggtttttc ttcaaggcac atataagtaa ttacagaatg
     7261 taaaaatatt gaaggctgag taacttttgt ttgcttcaaa tcttcgtctg taccgttaaa
     7321 aataatatca gttattctaa aacctaaaat ttcattagct ttttcaaaca tttcttttgc
     7381 tttagggttt gaattataca attcttgtcc catgccaaca tattgggagc cttgtcccgg
     7441 aaaaacataa gctttcatat tatagtattt ttagttcttt tatttaatta atgataaaaa
     7501 ttcctgacgt gttgcatctt ttaaaaatgc acctgtaaaa tcagaagttg tagtaattga
     7561 attttgtttt tgcactcctc gcatttgcat acacaagtgc tgagcttcaa taactaccgc
     7621 aactcccaat ggttgtaaag tattctgaat acaatctttt atttgtgttg tcaatctttc
     7681 ttgaacttgc aaacgtcttg cataaacatc aacaactctt gcaattttac ttaatccagt
     7741 aatataatga ttgggaatat atgcaacatg agcttttcca acaaatggca acaaatgatg
     7801 ttcgcacatg ctgtacaatt caatatcttt aacaattacc atttgcttat agtcctcgcg
     7861 aaacattgca gagcgtaaaa tttcttctgg attcatatta taaccttgag taagaaattg
     7921 cattgattta gcaactcttt taggtgtttt taataaacct tcgcgttctg catcttcgcc
     7981 taacaatttt aaaatttcag aataatgttg ggctaataat ctagtttttt catcatcata
     8041 tttttcaatt tttgcatagc caccattttc agaattattt aaactttcta ccatgtctaa
     8101 aattttttgc aaaaataaat aaaattgtcg taataataag ctatttttgt taaaacataa
     8161 attatgaaga ctttaataat tactgccaca agtttagagg ccaagcaaat agttgataat
     8221 ttgcacttta tcaaacgctc agacacagta ttctcaaaca caaattacaa ctgcgacata
     8281 ctaactacag gcgttggact tgttgctact atgttttcaa tgtttacttg cgtaaatatt
     8341 aaagaatacg atttgttaat aaatcttgga actgttggaa gtttttcgga aaatttaaaa
     8401 attggcgagc ttgtcaacgt taattcagat agctttgaag atatttgcat tagagataat
     8461 ggcaaaaaaa tcaatgtttt taattcaaaa tttaatcaac attttaggaa cttaattaac
     8521 aatggaagaa tttataatac aagcgattat ccacattttt ttcataattt aaagaaagtt
     8581 tctggtacaa gcgtaaatat tcctgagcaa aatcctattt caaattatga aatagaaagt
     8641 atggaaggtg ctgcttttat gttggtttgc aaacatttta agaaaaattt tattcaaata
     8701 agaggaattt ccaatattat tttaaaaaca aaacgtgaaa attgggattt taatactcct
     8761 attaataatt atagtgattt gactattgat tttttaaaac aaaacaaatg aaattaaaat
     8821 tagccatatc gccttgtcca aacgacactt ttatgtttga tgcgttgatt aacaaaaaaa
     8881 tagacactaa aaattttgat tttgagatta ttatagacga tattgaaaaa ctaaatacct
     8941 acacaaagca gcaagaagtt gatattagta aaactagctt tcataatttt ttaaaaattt
     9001 ctgataacta ccaattatta aattctggaa ctgcaatggg aaaaaattgc ggacctttaa
     9061 ttgttagtaa aagaaaaatt tatcctgacg agctaaaaga ctgtaaaatt gcaattcctg
     9121 gtgaatcaac cacagcaaat ttgcttatgc aaatattttt taatgaagca caaaacaaaa
     9181 atgtttacct tttttctgat attgaggaac ttgttctctc aaatgagtgc gatgcaggtt
     9241 tgctaattca cgaaacacgt tttacttatc aaaagcgggg tcttaaatta attgccgact
     9301 taggaaattt atgggaaaat aaatttaatc tacccattcc cttaggtgga ataattgtaa
     9361 aaagaagttt acccaacaaa ataaaacaag aaatagataa aatgcttagc gaaagcataa
     9421 acttagcatt taaaaatctt agaggcacaa tatcttttat gaaaaaacat gctgttgaaa
     9481 tggacgaaga aattatgatg caacatgtga atttatatgt gaatgaatat tcaaaagata
     9541 ttggcgactt aggaaaaaaa tcaatttata aattaagaga agagtttttg aaactgaata
     9601 atttagaaga taaaagtttg gatttgtttt tgtaactgaa gataaattga ttttcatcta
     9661 agcattttca gacttttctt tttgtggagc tggcgggaat cgaacccgcg tccaaacaag
     9721 aaacaaaggt gctttctaca tgtttatttt gcctttaatt ttcgagcctt agagcgagga
     9781 caaacaccca gactaagacc ttatccccta aactttcgcc tttttatcgg ggagataaaa
     9841 aagctagttc ggtagctatg ccatctcttt gccgaagcgg aaccgaaccg tccaatcgag
     9901 agatgtttcg tttcagcacc ttgtgccgaa attaagttaa tctactttat tcgattaagc
     9961 agcgaaagca aagttatctg cgccagttaa ttgtttgtga aaaagtttaa cgggattacc
    10021 cacattgccc gacatgctta cacaatcatt taccttgctg tcaaaaccga gtcagcccct
    10081 gatttgtaaa tttatgcaaa gatataaaat atttttaata ttacgatttt aaaattttaa
    10141 ttgtttctac tggattctct gaaccaaaaa ttgctgaacc tgcaactaaa gaattagctc
    10201 cagccttaat aatttcttta taattgtgag tatcaacacc accatcaact tgaattatta
    10261 agtttggatt ataaatatct gccatttctc tcaaattttt tattttaacc aaactttgac
    10321 ttataaaact ttgccctcca aatcctggat ttaccgacat aattaaaacc atatccaatt
    10381 ctggcaaaat atcattcaac aattctactg atgtatgtgg atttaaagac actccagctt
    10441 tcattccagc atttttaatt tcttgaactg tgcgatgcaa atgcgtacaa gcctcataat
    10501 gcactgttaa aatatctgct ccaacttttt tccattccgc aatatatctt tctggctgaa
    10561 caatcatcaa atgtacgtct aaaggttttt gtgcaaattt tttaatatat ttaactactg
    10621 gaaatccgaa agatatattt ggaacaaaca caccgtccat tatgtctaga tgaaaccaat
    10681 cggcttgaga ttcgttaatc atttgaatat ctttttcaag attagcaaaa tttgccgaaa
    10741 gcatagaagg agatagtatt atatttttca ttttgtgttt tttaaattta ctgcaaatat
    10801 aaactttttt caaaaaaatt taaaacttat acttatataa gataagattg caatatattt
    10861 cgctttgacg actgtctaag ttttttaagt gaagattctt ttagttgtct aatcctttct
    10921 ctagtaagat ttaaagcttc tgaaatttcc tcataagtct tagggctttc atcttctaaa
    10981 ccaaaaaaca tagtaataat ttttctttct ctttcactta atatttttaa agcttcatta
    11041 atttcaattt ttaaagaatc gttatttaag gttttttcaa cagaaagtgt ttgtgtggag
    11101 cttagtaaat cgcccattgt aaaatcatcg tcaccttcac tattacttaa aggagcgtcc
    11161 atcgacaatg ttttaccttg atatttaagc acttcattaa catttttaaa attatagtct
    11221 gtttcttcag caatttcggc taaactaggt tctcgttcta atttttgctc taaagctaaa
    11281 gaagttgaat aaactttatt taaaacacct attttattta gcggaagtct aatcattctg
    11341 ccttttaccg ctattgcatg caaaatagat tgtcgtatcc accaaactgc ataagatata
    11401 aatttgaatc ctctagtttc atcaaagcgt tttgcagcaa ctaataagcc gtaatttcct
    11461 tcgtttacta agtcttgtaa actcaatcct tggttttgat attgttttgc tactgaaaca
    11521 acaaatctta aatttgctaa aaccaatttg tcaaccgctt cttggtcacc atctttgata
    11581 cgttttgcca attcaatttc ctctgctgaa gaaatcattt cttcacgtga aatttcttgc
    11641 aaatactttt caaatgcaat actgtcccga ttagttacct gacgtgttat tcttaattgt
    11701 cgcatgtcct caaaattaaa ttcaatatat taaacgtttc attcgtcaaa aaggttgtct
    11761 aaaacagtat tttttttaaa caatttgaat taaatactaa aaaaacaagc gaatagtttg
    11821 cctatttagt aattttccaa atgttatttt taaaatagca caagcacata ttattttgat
    11881 tgtaaagtgt ttacttaaaa aatttttaat atagtcttga aaaatctcaa gtttttaaag
    11941 aatttatttt tattttaaaa aaaacatatt atttttgtcc cctgattaat taacgctgtc
    12001 gagttgtcat tttgacagtt taaaaaaatc ttttaaattt attgtatcac ataaattaca
    12061 ctcattcatg tatgacatta tagaactaaa ctcaaaatta ttacctgagt taaaagaaat
    12121 tgcaaaagat cttggtattc ataaagttga tgcatttcgc aaatctgatt tgatatataa
    12181 aattcttgat gttcaagcta ttaaagctgc tgagcaagag gcggataaaa agaaacaatc
    12241 taaagttcaa aattctgatg ctacaaacaa agctagtgaa aacggaaaaa gacctaggac
    12301 taaaatgaag gctcagaaaa taacttttag ctcccctact cctactcaaa agaatttgtt
    12361 tgatgaagta caacaagtta acaatacctt agaaacaaaa gaaaaagctg tagttgagga
    12421 gcaaaacaag actaaagaca ctgttgacaa taaaaaagaa gttgaacaaa aaaaatcaaa
    12481 tgcagtagct caaaatgctc aaccaaataa aaatttagct gaagcaaaac ctactaacaa
    12541 tgccaaaaac actcaaactc ctcatcaaaa aggacaagag aaaaaaatga ttttaaaaaa
    12601 gcctcaaact aagctaaatg atgaacttac gcatttgcct gatattgaga cagaaagacc
    12661 tatacaagcg gaatcaacaa aagtagaacc agcaaaagta gaaactacga aagtagaaac
    12721 tacgaaagta gaatcaacag aagttgttaa aacaaatggt gaaacaaaac aaaaagaaca
    12781 agaacctaaa aaacctaaaa aagaagattt atatgatttt tctggaatta ttacggttac
    12841 aggagtttta gaagttattc ctgaaggtta tggtttcttg cgttcttcgg attatcatta
    12901 tttgaattct ccagacgatg tttatgtttc tcaatctcaa ataaaattgt ttggattaag
    12961 aactggtgat actgtcgaag gttcaattcg tcctccaaaa gaaggagaaa aatattttcc
    13021 attaactaaa gttataaaaa taaatggaat agatcctgct ctaatgagag acagagtgcc
    13081 tttcgaccat ttaactccat tgtttcctga cgaaaaattt aatatcacag gtaatggaaa
    13141 agcaacttta tctacaagaa ttgttgactt atttgctcca ataggaaaag gacaaagagg
    13201 tttgatagtt gctcaaccaa aaactggtaa aactgttctt cttaaagaaa ttgcaaatgc
    13261 aatagcagct aaccatcctg aagtatattt aatgatatta cttatagacg agcgtcctga
    13321 agaagttaca gatatggctc gcaacgtaaa tgctgaagtt atcgcttcta cttttgatga
    13381 acctgcagaa agacatgtta gggtatcgag catagttctt gaaaaagcaa aaagaatggt
    13441 agaatgtgga catgatgttg tgattttact agattcaatc acaagattag cacgtgctta
    13501 taacactgta gctccagctt cgggaagagt tttatcagga ggtgttgatt caaatgcttt
    13561 acataaacca aaaagatttt tcggtgctgc tagaaatatt gaaaacggtg gttctctaac
    13621 aattatagca actgctttaa cagaaacagg ctctaaaatg gacgaagtaa ttttcgaaga
    13681 gtttaaaggt actggtaata tggaacttca attagacaga aaattatcta accgtagaat
    13741 atatccagca gttgacatta ccgcatcaag tacaagacgt gaagatttat taatagacaa
    13801 tgagctaatt aatagattat ggattttaag agaattccta gctgatatga actctgtaga
    13861 agctatggag tttatgagaa caagactaat gcaaactgca actaacgaag agttcttatg
    13921 gactatggac aaaggattat aagttaaaag ttaaaagcga tgcactgaac gtagttgaag
    13981 tgtcgaaccg agtttacgag ctcaccgaag gtaaagttaa aagcgatgca ctgaacgtag
    14041 ttgaagtgtt aaaagtaggc tgacgcgtct attgcttcgc agagttcgct tcgctcactc
    14101 tgctcactcg gatttagtta tgcttagcta gtcttgcgga catctgttgt aatcctagtg
    14161 ttttactcaa ctttacgaac tttataaact ttcaactgat ttgtccgtag gacaacatgc
    14221 aaacaacact tctattacgt tcagcacatc acttttaatt gaaataatta ttgcttacca
    14281 ataaattatt aattataaat tatcaattaa ataattattt taattttcca gatttttatt
    14341 taaatttgca atcttgaaac ttataagttt tgatttttaa aaacgaaatt aatgaagacg
    14401 agtaaattag gattagatca gaaaaaaatt gaatctgcaa gacaaattgc aaagaatatt
    14461 gcagacgaag ttcaagattt tgttaatcaa tatactactg ttgcagtaga gcgctcactt
    14521 tgtcgccttt taggaataga cggagttaat tctaacgaca tacctttacc aaacgttgtt
    14581 attgaccaaa ttcaggaaaa caagtcgcta agtgaaggta ttttattttt tcttggaaat
    14641 gcaattttag aaactggatt aaatccacaa caaatagccg aaaaaattgc aaacgaagat
    14701 tttaacattt gcaaacttcc aataagagat aaagcaagca ttgaaaaagc tataatgcca
    14761 tacgtaaatg ctagcgttga aaaaatcaag aaaaatgttg caattcgtaa tgaatatctt
    14821 tctcgcttag gcgaaggtag taaaccttat atctatgtaa ttgttgccac aggtaatatt
    14881 tacgaagatg ctattcaagc tcaagctgct gctcgccaag gcgctgatat tattgcagta
    14941 attagaacta ctggacaaag tttattagac tatgttcctt atggtgctac taccgaaggc
    15001 tttggcggaa cttttgccac tcaagaaaac tttaaaatta tgcgtgcagc attagacgaa
    15061 gttggcgaag aagttggacg ttatattcgc ttgtgcaact actgttcagg attgtgcatg
    15121 cccgaaattg ctgctatggg agctatagaa cgactagacg ttatgttaaa cgacgcactc
    15181 tatggaatct tgtttcgcga tataaatcaa caaagaacaa ttgttgacca atatttttca
    15241 agagttataa atggatttgc aggcgtaatt ataaatactg gcgaagataa ttatttaaca
    15301 actgctgatg cttatgaaca agcacataca gttttagctt cagaccttat aaacgaacaa
    15361 ttagcactac ttgctggaat gcctgaagaa caaatgggtt tgggacatgc ttttgaaatg
    15421 gacccagaaa tggaaaatgg atttttatac gaacttgctc aagctcaaat gactagggaa
    15481 attttcccaa aagctccttt aaaatatatg ccaccaacaa aatttatgac tggaaatatt
    15541 tttaaaggac atattcaaaa tgcacttttc aatgttgtat ctatttggac acaacaagga
    15601 atacaacttt tgggaatgtt aaccgaagct attcacactc catttatttc tgatagatat
    15661 ttagctatag aaaattcaaa atatgttttt aacaacttaa aaaatattgg agacgaagtt
    15721 gagttcaaag aaaatggaat aattcaaaaa agagcgcaag aagtactaaa caatgcacat
    15781 gctttattga gcgaaattga taaagttgga ttatttgatg ctttagaaaa aggattgttt
    15841 gccgatacta aaagatcaaa aactggtggc aaaggattag caggtgttgt acaaaaatcc
    15901 gaaaattatt ttaacccatt tatcgaaata atgaaaggag gaaacaaata atgagtggcg
    15961 gaaattattc aatggataaa aaagaatttg ataaaatatt agaccttaaa aaagtaaaac
    16021 catatggaga taccatgaac gatggaaaag tccagcttag ttttactttg ccagtacctt
    16081 gtagcgacga agcaagtgag gcagcaaaac aactaatgag aaaaatgggt tttgaagaac
    16141 cacaagtagt ttatttcaaa gaacttacaa aaggatttac tttttttgtt tgttatggtt
    16201 cttgcactca tactgtagat tttacaagca ttcacgttcc aaaagtagaa tcaaatgtga
    16261 tgaacatggc tgaaacagac gaattcataa gtcaaaatat aggaagaaaa ttagttgtta
    16321 ttggagctag tactggaaca gatgctcata ctgttggtat tgatgcaatt atgaatatga
    16381 aaggctatgc aggacatttt ggtttagaaa gatacgaaat gattgaagcc ttaaatatgg
    16441 gtgcacaagt tttaaacgaa gattttattg ctaaagcaat tgagttaaaa gctgacgcat
    16501 tattagtttc gcaaacagta acacaaaaag atattcatat taaaaacctt accgaattag
    16561 ttgaaatgtt agaagctgaa ggactaaggg ataaagtttt attgatttgc ggtggaccaa
    16621 gaatttcaca tgagttagca aaagaattgg gctacgatgc tggctttgga atgaactctt
    16681 atgctgatga tgttgcaagc tatatagctc aagaaatgga tagaagaatt aataataaat
    16741 aaaattttaa agcatggaca aaaatcaaat gcgagaagta atcgcaaaac gagttgccaa
    16801 agaaataaat gatggcgata ttgtaaattt aggcattggt attcctactt tagttccaaa
    16861 ttatttgcct gaaggagtaa aagtaacctt acaatctgaa aatggattgt tgggtatggg
    16921 accaagtcct gaaccaggca aagaagacga aaacttagta aatgctggag gtggttatat
    16981 tacagctaat ctaggtgctg catcatttga ctcttcacga tcttttggta tagttcgtgg
    17041 tggacatgtt gacgtaactg ttttaggtgc tatgcaagtt gacccaagtg gcgacttagc
    17101 aaactggaca attccaggaa aattaactcc aggtatgggt ggtgcaatgg atttattagt
    17161 tggtgctcgc agagttattt tagcaatgga acatactgca aaaggaagac ctaaaatttt
    17221 aaagaaatgt acactaccac taactgcaaa aggacaagta aatacaatca ttaccgaaat
    17281 gtgcgtaatg gaaattactg aaaaaggaat tgtattaact gaaatacatc cagaatttac
    17341 tgttgaagac attaaagcag ttacagaagc tgattttata gtatcagaaa atctaaaaga
    17401 aatgcaatta gattaatgca ataaatttta attaacataa acttattata aaaatgaaaa
    17461 aagtattttt aattgaagct aaacgtactg ccattggaaa atttggaggt agtttggcta
    17521 cagtttcggc ttccgacatg gctgctgaag ttataaaaac aattatttct aacaccggca
    17581 ttaatcctgc cgatttagat gaagtaatca caggaaatgt tttgtcggca ggacaaagac
    17641 aaggcgtttc tcgtcaggct gcaataaaag caggaattcc aaacgaagta ccagcctatt
    17701 ctttgaatat ggtttgtggt tctggtatga aagcggtttc aaatgctgtt tctgcaatta
    17761 gagctggcga agcaaactta attttagcag gtggtacaga aacaatgtct ggtgctccaa
    17821 tgctattacc agcaagtgtt cgtacaggta caaaaatggg cgactttaaa gttattgacc
    17881 acatgatatt agacggactt tgggaagcat ttaatgatta tcatatggga aatactgctg
    17941 aaaatattgc agaaaaatat aatatttcac gcgaagaaca agatcaattt gcatttgcat
    18001 ctcaaactaa agcagcaaaa tcccaagatg ctggaaactt caaaaaagaa attattccta
    18061 ttaacattgt aactagaaaa gaaaccatag tttttgacca agacgaattt attaatagaa
    18121 ctacagactt ggaaaaactt gcaaaacttc gtcctgcttt caaaaaagac ggaacagtaa
    18181 cagcaggtaa tgcatctggc atcaatgacg gagcttcata tgtattagta gcaagcgaag
    18241 aagctgttga aaaattcaaa ctaaaaccaa tggcagaaat tgttgcggtt ggacaaggtg
    18301 gtgttgaccc tgcaattatg ggaatgggac cagttcctgc aattaacaat gtattgaaaa
    18361 aagcaaacat gaaacttcaa gacatagaag ttcttgaact taacgaagct tttgcagctc
    18421 aatcattagg agttatcaaa caactttgca cagaccacgg agttgacaaa tctttctttg
    18481 acgaaagatg taataaaaac ggtggtgcaa ttgctttagg tcatcctatt ggagcttctg
    18541 gaaatagaat tattgtgagt ttaattcacg aaatgaaaag aagtaataac aaatttggtt
    18601 tagcatcact ttgcataggc ggaggaatgg gaactgcaat tattttaaaa aacatttaaa
    18661 agtttaaaaa ggtgagtaca acatatttaa ctcacctttt ttttattttt catatttatc
    18721 tttggaaata aaatataaat ttataacttt accttgttaa atcacaaata aaaaaattat
    18781 gaaaaaaatt agattatcaa gcacaattat agtgctagtt ttagttgcgg ttttctttgt
    18841 ttcctgctca aaaaacaatt cttctcacat tccaaaaaat gcttttgtag tagcggttat
    18901 tgatggtaaa ggtcttaaag atttgacaaa taacaatttc ttaaaagaca atgaagaata
    18961 caagcaaaca atggaatctc ttaaacaaga aagtgaaaaa ttagtggagt tgattgaaaa
    19021 aacacaaaaa aatcctgatg aattaggaat tttattaaca aaacgttctt acgcttttgc
    19081 aagctacgac aaagaagaac tactctatgg atttattatt ccaataaaca aaaagaaact
    19141 tgaagcaaac ttagatatga taggctctga gttaggcttc cctatttcag cagttttatc
    19201 aacaaaagac gagattaaat attttgcaga ctcagatatg attcttggat ggaacaaaga
    19261 tgtatttata tttttaggaa aagaaagtgg agaagttgat tttgagttat tagaaaaaca
    19321 ttttaacttg aaaaaagatg aatcaatctt agctaacgat gactttaata aattccacaa
    19381 aaattgtaaa gaccttaact tatggattag ttctgacatt ataaaaaatg ttgaagaatt
    19441 tgatagtgaa attaaggagt ttgaaacatt aacaggcatt gacctagcaa acaattacgg
    19501 tcatatgcat ttagaatttg caaaaggtga aattaatttt acatctagcc taagatataa
    19561 taaatcaatt caagaattag acatggagaa acttactgaa aattctgaaa ccctagaaaa
    19621 gtttttaagt aaatatacag attttataaa tatttgcggt cttaacaaca agtacgctga
    19681 ggaatacgat tatgactatg attatgaata tgacgaagat atcaactggg aagactacca
    19741 aagcgagttc acagaagaag aatgggaaga atttttaaaa gctttagaag aagttgaata
    19801 atctatttgg tatatttata tggaaataag attttccaaa ataatcccta ttccaattcc
    19861 tgaaagcaat attgctaatt ctgaaatttg gaatagggat ttattatttg aacaaaataa
    19921 taaatattta atctacgccg attctggcaa aggcaaaaca acttttgtaa atatcatttt
    19981 tggcttacga aaggactata ctggcaatgt gtatattgat ggagaagaca tctcgaattt
    20041 tagcataaac caattttctc aattaagaaa aacgcggctt agtattgttc cgcaagggct
    20101 tcaattattt ccagaattaa gtgttttaga aaacattaac atcaaaaata gaattcaaaa
    20161 ctacaaaacc tcaaaacaaa tatcagaaat gattgaaaaa ttaggactaa gtggttttga
    20221 aaatagaaaa actgcaaaaa tgtcgttcgg acaaaagcaa agagttgcaa taattagagc
    20281 actctgccaa agttttgact atattttact tgatgaacca tttagccact tagatgcaaa
    20341 aaacacagaa attgcatggg atttaataaa atcagaagct gagcaacaaa atgcaagcat
    20401 aattgtaact tctttaaaac aagattttga aacaaatata ataaagttga gggtgtaatg
    20461 atttggaaag ttttaaaaaa taatataaaa cctgctcagc taataggtac attttttggc
    20521 ataacgctag gcttgagtat tttaatggta gcggtgtcgt tttatcttga tgtaaaacca
    20581 atatttgaag ataaagaaag tttttggcgt gacgagtata ttattgtaaa taaaaaagtg
    20641 aaaatcacag actcatatca ccaaattaga gaagctgaca aagaaaaacc atatttcaca
    20701 gaagaagaaa tagaagacct aaaaagacaa gcctttgtaa aagatgttgc agaattttca
    20761 aactgctcat ttgccataag tgcttcggct gaccgagaca gtcctctaag cggattttac
    20821 acagatttat tttttgaagc agttcccgac aactatgttg atgtaaatta taaaaattgg
    20881 aattggactg aaaatagtgc ctatatacct gttattttgc caaaagttta tctaaattta
    20941 tacaatttcg gatttgctca aagtcaaaat ttaccacaaa tttcagaaag tggagcttcg
    21001 ttggcaaaat ttaacattca tgtttatgga caaaaaaagc accaaaagtt tgaagctaga
    21061 attgttggtt tttctgaaag aataaataca attttagcac ctaaatcatt tgttgactgg
    21121 ggaaacaaaa attttggcga gcatgaaaaa tcaaaacctg gcagaattat tattattgca
    21181 aaagacccgt caagtcaaga actagttgaa tatattgata atcacgacta cgacataaac
    21241 aagtcagaac ttagtaatag caaagcacta gcatttctaa aaataataat aggaatagtt
    21301 gtaacaatag gcattattat aatttgctta acattcgcat taataatttt atcaatacaa
    21361 ctattgctgc aaagaaataa cgagaatatt gttaaactaa atatgcttgg ttattcttta
    21421 aaagaaattt cgctacctta taaaataatg gtaagcatat tgtttgctgc aagttttatt
    21481 agttctctta tcccattata ttttttaaga aattattatt tctctaattt agttttattt
    21541 ggatatgaaa gcactactca actgtttagc aatatcatat taccttcttt aatatttatt
    21601 tttatagtgg tactattatt aataagtatg ctaaaatcaa caataaagaa aattatttct
    21661 aaaaattaaa ttgaactaat ttcctgcacg tttggagtaa tcaacaatct ataaaaaatc
    21721 aaatcaataa tttgtcaaat tatttgttta ttattatttt tgtttcggta aaacaatatc
    21781 acgctatttt tggtttgtat aaaagtttcg aatagttttg atatttttgt aaaaatttta
    21841 aaataaataa aatgaaaatt tcaataattg gtacagggta tgtgggttta gtttctggta
    21901 catgctttgc tgaaatggga ataactacat cttgcttaga tgttgaccaa caaaaaattg
    21961 aaaaccttaa aaacggtata atgcccattt gggagcctgg attaaaggaa atggttaaga
    22021 gaaatttcga aaaacaaaga ttgagtttta ccacaaacat ttcagaaagt ataaactcgg
    22081 cttctgcttg ttttattgct gtaggtacac cacccgacga agatggatct gccgatttac
    22141 aatatgtttt ggctgttgca aaagaaattg gtcaaaacat aaataattat atggtcgtaa
    22201 ttataaaaag tactgtccct gttggcacaa gcgaaaaggt aagagcaact atacaaaagg
    22261 aaattgaaaa acgaggagtt aatataaaat ttgacatagc ctcaaatcca gagttcttaa
    22321 aagaaggaga tgctattgaa gactgtcttc accccgaccg cattatagta ggaacagatt
    22381 ctgaaaaagc aagagaaata atcaatagaa tatactcgcc atttgtatta aatggacatc
    22441 caattattca catggatatt gcatcagcag aattgacaaa atacaccgca aatgcaatgc
    22501 ttgcaactaa aatatcattt ataaatgata ttgcaaattt atgcgaaatt gttggagcag
    22561 acattaatgc tgttagaaaa ggaattggca cagataaaag aattggacca tattttattt
    22621 atgctggtac tggctatgga ggttcatgct ttccaaaaga tgtaaaagca attattaaaa
    22681 ccgctgacga acatggacat tctttagaaa ttttaaaatc tgttgaaaat gttaataatc
    22741 gccaaaaaga aatacttttc cataaagcaa acaaacattt tgaaggaaat ttgaaaaata
    22801 aaaaatttgc aatttgggga ttatctttta aaccaaacac agacgatatg cgtgaagctc
    22861 cttcaatcat attaataaat aatctttggg aagcaggagc tgatatttat gcctacgacc
    22921 cggttgcaat gaaagaagca aaaaaaatac ttcctaaaaa agtaaaatat tgcaaaacac
    22981 cttacgaagc agttgaagaa gctgacgcat tatttttagt taccgaatgg acagagttta
    23041 gggttttaaa ctataaagca ttagacaaaa tgaaaaacaa agtaattttc gacggcagaa
    23101 acatttatca acccgaagaa cttaataaaa acggttttac atattatgga ataggaagat
    23161 aatactatta tgagtaaaat tgaagttaca atttgcctag gctcctcctg ctttagcaga
    23221 aaaaaaagag atattgtaag tgctttacaa gactatattg atcaaaaaaa tttgtcaaac
    23281 agaataaatt taaaaggtgg gcattgtttg ggaaaatgca gtgtaggacc aattatagtt
    23341 gtaaatgaac aaatttatac cggaataaca ccagctaaag caatacgcat aattgaggaa
    23401 tatttatcaa ctcaaaataa ttaaaatgca actaataact tttgataaag aaaaatgcct
    23461 ttcttgctat gcatgtgtaa aagcatgtcc tgtaaaagca attacaataa gttcttcaga
    23521 ggaaatttta agtttcaatc acaacagatg tttaggttgc ggaaactgtt atattagttg
    23581 tggaccaaaa gctatcggct atattgattc tacagaagca gtttcacaac tattaaattc
    23641 agaaactcct tgcattgcaa ttgttgaccc atcaatatcc gcagaatttc cagatatttt
    23701 agacgttaga aattttgtgg gaatgattag agctttaggt tttgaatttg ttaatgaaat
    23761 ttcgtttggt gctgatgtta ttgcatataa atacaaaaac ttattcgata attttaaagg
    23821 aaaatatcat ataacaacaa attgccctcc tatttatgat tatgttgaaa aatttcaaac
    23881 tcacataatt gattcgcttg taaaaattga ttccccaatg attgctacgg caaaaattat
    23941 gaaagaaata catggagatg actgtaaaat tattgccata acaccttgta tagctcaaaa
    24001 acgcgaagca aaaagatttg gctttgttga tgaagtaatt agctttgaag aattgcgaat
    24061 attgtttaat caaaataaaa tttatgaaaa ttcagtggag ttttcagaat ttgacatgcc
    24121 cttaggtgct aaaggctcat tatatccgct tagcacagga attttaaatg ctgccgatat
    24181 taattataca gatattgatt ctaaaataat tactaaagac ggggctgagc attttaccca
    24241 atttatgaac gactttgcaa gtattagttt aataaagcac catttgaata ttttttattg
    24301 tcatggttgt gctatgggac ctggctctaa aaacaataat agctatttac gaaatcatag
    24361 cttagttgtt gattacacta aacgaagatt aaattctgct gttgataaac aagaatggga
    24421 aaaaaatctt gaaaaatttt cgaaattaga tttcactcgc agttttactg ccaacgacca
    24481 acgcattcct accccatcac aagcaaaaat aaaagaagtt attgatttgt taggtaaaga
    24541 tcaactttat gaagttggtt gtagaagttg tggttataaa aattgttctg aatttgttac
    24601 agatgttgcc aaaggtttag cacgtcctga aatgtgtcaa aacttttcaa aaacaaataa
    24661 acaaaactat ataaataaaa taaatattgc aaatgccaac cttgaagaaa ctaagaaaaa
    24721 ccttgaagaa ttagaaatca aattgcaaaa agagcataaa gatgtaaaag taatggcaac
    24781 tttaacaaaa gcaataatag aaaaaattcc ttcggcagta attgttgttg acgacaaatt
    24841 aaagatactt caatcaaacc aaaaatttat agagctttta ggacaagacg ctaaagaaat
    24901 tgatgaagta gttccaggac ttattggagc agacattaaa actttgttac ctagtcaaat
    24961 ttataatttc tttagttttg ttctcaacaa agacgaaacc attgataata aagatgttga
    25021 aattgatgat aaattaatta cagtttctat tttcccaata gagaaaggca aaactatagg
    25081 tgcagtattt aaaaatttat atcaatcaga agttagaaaa gaagaagtaa tgtatagaat
    25141 taacgatgta attgaaaaaa atctaggaat ggttcaacaa ataggttttt tattgggcga
    25201 aggagcttct gaaacagaaa gaatgttaaa tagtattatt aattcttttg agaaagaaga
    25261 taaagaagaa aaataaaaaa acatgtctga aaagctatac atagaagttg atgttaggca
    25321 aataaactat cacggcgaaa gaatttgtgg cgatgtcttt cttaaaaaac gagtaaaaga
    25381 agacaataga attattgctg ttttaagtga tggtatggga catggcgtta aggcaaatat
    25441 tttagctaca ctaacatcta ccatggcttt aaattttact atagaacata aaaaacctga
    25501 aagaattgca gaaataatta tgaacacttt gcctgtttgt agcgaaaggc aagtttcgta
    25561 tgcaaccttt acagttgttg acatagagtt tgatggacaa gttcatattt tagagtacga
    25621 caatccagct ttattagttt ttagaaaaaa taaattacta aaattacctt acgaaaccat
    25681 tgaaatgaaa aatccacctc atgctgggaa aaaattaaca tatacaagtt tttatccaaa
    25741 attgcatgac aggcttgttt tttgttctga tggagtttct caaagtggaa tgggaaccga
    25801 caaatatcct tttggttggg gagttttagg aattgaaaaa catgtaacag gcattttaga
    25861 aattacaaaa gatatttctg cttcaaaact tgctgaaaaa gttgtgaata aagctgttgc
    25921 taacgatatt tatattccaa aagacgacac ttcttgtgct gttgtttatt ttagaaaacc
    25981 acgtaaactc gttattgcca caggacctcc aacgtacaga gaatcagaca aagaattagc
    26041 tgagtttata aaaaattatg acggaaaaaa aattgtagca ggaggttcaa caactgattt
    26101 gattgcaaga gaacttgatt taaaagtaag cacatctctt gaatttgcag atccagaatt
    26161 gccaccaatt tcacatatag agggaattga cttagttaca gaaggaattt taacactaaa
    26221 taaagtttgg aaaattttaa ctgaatatga accccaatat aaattaggca gaggaccagc
    26281 cgaccaaatt gtaaaactaa tattagaaag cgaccatatt gatattgtgg ttggaacaaa
    26341 aatcaacgaa gcacatcaag acccaaacgt tcctatagat ttagaaatca gaagaactat
    26401 tgcaaataga attgctaata tattagaaaa taaatttcta aaacatatca caataaaata
    26461 tttctaacaa gcagaaaact aaatttgcaa aacttttaaa cttacttaca aaatatagtt
    26521 gaagaattga atttgttttt tatttttgca acaaattaaa tgtttatata taaatgtttg
    26581 agtacataaa aggattatta acagaattaa gtccgactta tgcaataata gaagctaatg
    26641 gaatagggta ttttataaat attacagtaa atacatattc taagcttaaa aaagggcaag
    26701 aatgtaaatt atttttacac gaaattcttc gcgaagatct tcatgctatt tacggttttg
    26761 aaaatcttag cgaacgcgaa atgtttagaa aattaatcac tgtaaacgga gttggtgcca
    26821 atactgctag attaatttta tcatcgctaa gtgtaaataa tgttgttcaa gcaattaacg
    26881 aagaaaacat aaatgtttta aaatctgtaa aaggcattgg agttaagaca gcacaacgaa
    26941 ttataataga attaaaagat aaacttagcg actctgatat acaaatttca gaaatttctt
    27001 caagtttaaa caatacttta caaaaagatg cgttaaatgc tttaacaatg ttaggttttt
    27061 cgaaaccgaa tgttgaaaaa gtgttaatta aactattgaa agacaataag gaaataagtt
    27121 tggaaaatct tattaaattg gcgttaaact atttataaag aattgctttg ataacaaaga
    27181 tttttaaaca tacattttta tttagcttaa taataggaat tttttctatt atatttgctt
    27241 ctaatgctaa attaaattac aatataccta agcgtaatcc aatcttaatt gataattttg
    27301 atgatgatac cattaaaggt gctaagttaa attatcctat tccagaagag gatattaatc
    27361 ccgcaaataa taaagacaaa agtcctcttt atggttctga tccttcgaat attgaaacag
    27421 aagttgttta cgatgcagaa tcaaatcaat atatttttca aaagaaaata ggtggcgaaa
    27481 attttggaac tccttattca atgagttttg atgaatattt agactacgac tttgaaaaag
    27541 ccatgaaatc atattggcga caacgttcta aagatgacat ttcagaaaca agagatgttt
    27601 taattccaaa attacaagtt gatggcgaaa tatttgatag aatttttggt ggaagcaata
    27661 tagatattag acctcaaggt tctgcagagc ttatttttgg attaaaagtt gataaaattg
    27721 aaaacccagc tatgccactt cacgctcaaa gtcctgttac ttttgatttt caagaaaaaa
    27781 ttcaaatgaa cgttgttgga caaattggag aaaaaatgaa agttaatctt caatatgaca
    27841 ctgagtcggc ttttgatttt gaaaactctg taaaacttga atacacagga cacgaagacg
    27901 aaattataca aaaaatagaa gcaggaaatg tatctttgcc attgacagga actttaatta
    27961 ctggtggtca aagtttattt ggtttaaaaa cagaagcaaa atttggaaaa cttactgtta
    28021 caacgctatt ctctcagaaa aaaggagaaa tgtcaactgt acgggttgaa ggcggtgcac
    28081 aatcccaaga ttttgaaata aaagctgatg aatacgaagc aaacaaacac tatttcttat
    28141 ctcattattt taaagaaaat tatgatagag ccttagctca acttccaatt ataaatagtg
    28201 gaataaatat cacacgtatt gaagtttggg taacaaacaa agctggtaat tttgaaaatg
    28261 ctagaaatat tgttgctttt gctgacttag gtgaaagtaa cgataacaac atacaatccg
    28321 attatgttag aactgctaac ggtaacacga caacagtata tcctcattct gaaattaata
    28381 ttttaggaga gataacaacc acctatcccg atgttagaaa tattaacaac gtaggaaata
    28441 cattattagg tttaggaatg acaggcggaa gagattacga aaaaattgaa tcggctcgtt
    28501 tgttatcacc caacgaatat actgttaatg aaagattggg atatatttct ttaaatgcta
    28561 ctttaaatcc cgaccaagtt ttagcagtag cttacgaata tacagttgca ggaaatgttt
    28621 ttaaagttgg ggaattttct aactctgcaa ttacagctcc tgatgcttta gttgtaaaat
    28681 taattaaagg aacagcattt actccacaat taaaatcatg gagtttgatg atgaaaaaca
    28741 tttacaacat aggtgcgtat ggaataacaa acgaagactt ttggcttgac attatgtaca
    28801 ccaacgataa aactggtacc gaaataaact atattcctgc aggtgctatt gattctattc
    28861 gtcttcttga agttttaaat ttagataatt taaactcgca acacgacccc taccctgatg
    28921 gaatttttga ctttatcgaa ggatacacag tttcaacaag caatggaaga atgatgtttc
    28981 cagttacaga gccatttggt tcatatttaa agaaaaaaat tactggcgga gacgcttctt
    29041 taaataatat tgcagaaaca tatattttcc aagagttata cgatagtacg caaaatacag
    29101 caggacaaat tgctgaaaaa aataaattta gaattaaagg gaaattcaaa tccgctggtg
    29161 gtagcgaaat tagattgaat gttgcaaata tccctcaagg tgctgttatt gttactgctg
    29221 gagctgttaa attagtagaa aatgttgact atacggtaaa ctacaacttg agtactgtaa
    29281 aaattataaa tcaaggaatt ttagattcaa aaactcctat tgacatttcc ttagaaagta
    29341 attctggttt taatatgcaa accaaaacct taataggtag tcatgttaat tatgaatttt
    29401 ctaaagattt taacataggt gctacaattt taaacttaac agaaaaacca ttacaaacta
    29461 aagttggtat aggttacgaa cctatttcaa ataccatttg gggttttaat acatcatata
    29521 gaaccgatgt gccattttta acaaaaatga ttgactttat accattgatt gaaaccaagg
    29581 aaatgtcaac attaactttt actggtgagt ttgctcattt aattcctgga cactcaagag
    29641 caattaaaaa agaaggaaac gcctatattg atgattttga aggaacaaaa acaagcttag
    29701 acttaaaatc tcaatctgca tggactttag caagtacacc tgccgatgta tccatgtttc
    29761 cagaaagtgt attaacaaat gatttaagat atggatttaa tagagcaaaa ctaaattggt
    29821 ttacaattga gcccatgttg ttaaaaccta acaactcgcc tgtttctatt gaagaccaat
    29881 caagccatta tgttagagaa gtttatgaaa aagaattatt cccaaataga gaatctcaat
    29941 catctatacc tgtacattta tacacactaa atttagctta ttatccacac aaaaaaggac
    30001 cttacaactt tgatgttgac ggaacaaatg ctgatggtag ccttaaatat ccagatacaa
    30061 gatggggagg aattatgcga ccaattcaac aacctgattt tgaacgtgat aatatagaat
    30121 atattgaatt ttggttaatg gatccttttg tagaagattc taccaatgtt ggaggagatt
    30181 tattttttaa cctaggagat gtttctgaag acgtgctaaa agacggacgc aaatcttttg
    30241 aacaaggatt gccaactcct tcaacagata atcctgttga cacaacagct tggggtttag
    30301 taccattaat gcaagcctta gttcccgctt ttgacaacga tgcagaatca cgtgtagctc
    30361 aagacgttgg ttatgatggt cttagcacag aaaaggaaag agaattcttt aaagcatatt
    30421 tagacagctt agctgcaaaa catggagtta attcacctgc ttatatcaaa gcttacaatg
    30481 acccttcaaa tgacgattac catcacttta gaggctcaga ttacgatagc gacaatctag
    30541 gaattcttga aagatataaa ctttacaaca atccagaagg aaactcacca ccagcagaat
    30601 tttcacaaga aaattattca actagtggaa aaaccgaccc taatgtagaa gatattaata
    30661 gagacaatac attaaacgaa ggtgaaaatt atttccaata cagagttagc attcgtccac
    30721 aagattttga agttgggaaa aattatatta ccgacaaagt aattgtatca tcaacttttg
    30781 taaacgacaa acaatctaaa gtagcttggt atcagtttaa aattccaatt cacagttatg
    30841 ataaaagaat tggtaacatt caagatttta aatcaataag atttatgcga atgtttatga
    30901 caaactttac tgctgaccaa actatattaa gatttgcaac tttagattta gtacgtggcg
    30961 aatggagaaa atacaacgct tcgtttatgc aacccggcga atatatagta gatgaacttg
    31021 ctgatacccc ttttgaagtt agtgcagtaa atattgaaga aaactctaat aaagttcctg
    31081 taaactacgt attgcctcca ggaatcaatc gcgaatcaga cccaatgaat ccacagcata
    31141 gacaacttaa cgaacaatct atgtcgctaa aagtttgtgg actacaagat ggagatgcaa
    31201 gagcagttta taaaaatgta aatcttgacg ttagaagata tttacgttta aaaatgttta
    31261 ttcatgctga atcagtacct ggcgaacaac ccattcaaga caacgacatt tcggtatttg
    31321 tacgattagg aacagattat aaaaacaatt attacgaata cgaaattcct ctaaaagtta
    31381 ctcctaatgg cagatatgac ggaactttag acgaacaagc tcctgataga tatattgtat
    31441 ggcccgaaga aaatgacttg aacttagatt ttgatttatt gcaacaagta aaacaaagca
    31501 gaaacgacaa aattagaaat ggagatccaa acgtaagcat aacaagacta tattctatgg
    31561 ttgatggaaa caacaagatt agtattatgg gtaatcctaa cctttctaat gttcaaacaa
    31621 ttatgatagg tattcgtaat cctaaaaaaa gatcagttaa cgacacagac gatggacaac
    31681 caaaatgtgc tgaagtttgg gttaacgaac ttagattaac aaattttgac gaaagaggag
    31741 gctgggcagc aaacgctaga ctaacagcca aacttgctga tttaggctct gttactgttg
    31801 caggtagtac aagtaaacca ggctttggct caataaatca aaaaattgga gaaagacaaa
    31861 aagaagaaat aaatcgctac gacatttcat caaattttga actaggaaaa ttcttccccg
    31921 aaaaattaaa tgttagaatt ccaatgtata ttggatattc ggaaaatgtt aaaaacccag
    31981 aatttaaccc actagaccct gatataccat ttaaagtagc tttatcagac ccaaatctaa
    32041 caaaagaata taaagacagt attaaacata tttctcaaga ttatagcatg agaaaaagta
    32101 ttaacttctc aaatgttaaa gttaataaaa cttctggcaa atcaaatatt tacgaccttg
    32161 caaactggtc tttaagttat ggatataacg agacatacca aagagatgtt aatactattt
    32221 acaacacaac taaactttat caaggagcta taaactacaa ctataatgct actccaaaaa
    32281 ttgttgagcc tttcaaaaat gtgaaattat ttaataaaaa ggctttccaa ttaattagag
    32341 attttaattt ctattattta ccttcacaac tttcgtttag aacaaatata aatagaagtt
    32401 atggagaaac tcaattaaga aatgtttaca attcttcatt agcactacct gtaaatgtta
    32461 gaaaagattt tacttggata cgtcaatatg attttaaatt caatattacc aaaaatctta
    32521 aattagattt tactgcaacc aataatgcaa gaatagacga acctcaagga agaatttata
    32581 aagatgatcc tttctatgaa gaaaaaagag atactatttg gaataatttc aaaaattttg
    32641 gaagaaatac acaatatcac caccaatggg atttaacata taacctacct attaataaaa
    32701 ttccattatt aaactgggtt acggcaaata ctagatactc aggctcttac gactggataa
    32761 caagtccaat tacagccgat acaataaaat taggaaacac acttcaaaat ggtaacacaa
    32821 tggctgtaaa cactcagttt aatttattag gattatataa taaaatcaaa tatttagacc
    32881 aagtaaataa aaaatataga ggtcgtcaaa gttctagacc caaaaagaaa gaagttgaaa
    32941 acgttacatt cgaacaaaca aatgtaaatc taaaagcaga taaagctaaa aaaattaatc
    33001 acaaactaaa aactgaagat gttaatatca aggtaatagc ccctgatgga gttgaaattg
    33061 tgggtgaaat gaaaattatt gatgccaata gaattgaatt tacaccaaaa aatgacgtaa
    33121 aagatgcgac acttactgta actggaaaac gagagaaaaa agaatctatt ccaaaaatta
    33181 tttttgacaa cactttagtt gtgttaatga gtgttaagaa catttctata gcttatacag
    33241 aaactaatgg aactttatta cctggctata tgccaaacac ttacattatg ggtatgtcga
    33301 aatatatggc tgatcctgaa atgtttggac ctactccttc tatcttaaca cctacaattc
    33361 catttatttt tggctggcaa gaagacggtt tttcagactg ggcgttaacc aataatgtaa
    33421 ttactaaaga cactactaat ctacaagcat ttatgcttac taataacaga aattggaatg
    33481 taagagcttc gatagaacct tttagagatt tacgtataga ccttaattta actcattcat
    33541 ttagtcaaaa tagaagtaat ttctttagat acggatacga tgctagccaa aatgctgatg
    33601 cttacatgac tttaaatcct tctcaaatgg gaagtttctc aatgtcaaca attacttggg
    33661 gttctgcttt tgaaccttta aagaaaggcg gagattatag ttcaaaagta ttttcacaat
    33721 tctcggaaaa cagaattatt attgcccaca gactagcctc acaacgacca ggctataatg
    33781 ccgatgatat tgatacttat ggtttcccat cagggtatgg aaaaacttca caagacgtgc
    33841 taatcccagc ctttttagca gcatatacaa ataaagatgc aaaagaagtg ggtttagaaa
    33901 ctttcccatc gatattgaaa gccttgccaa actggagaat tacatacgac ggcttaggaa
    33961 aaattcctgc tctaagaaaa atatttagaa cagttaatat tacacataat tataggtcaa
    34021 catttaatat tggttcatac aaaacaagat tagaaaatga atactatgaa tctgacggtt
    34081 tcaatattat acgagacgaa atagcgaact actattctaa atatgaaata aacggaatta
    34141 gtatttcaga acaatttgct cccctactat ctgttgatgt aacaatgatt aatagcttta
    34201 ttgtaaaact agaactcaaa aaaacaagaa atttgaatat gagttttagc aataaccagc
    34261 taacagatat gagaaataac gaatacatta ttggaacagg ttatagattt aaagatgttg
    34321 aaataaatat taagagcggt ggaagaacaa gaaatttcaa gagcgactta aatgtaagat
    34381 tagatttttc actaagagac aatcttactg ttactcgtaa acttgaagaa ggatttaatc
    34441 aacctactgc aggaactcaa acttatacaa ttaaggcttc ggcagattat gtattaagta
    34501 accgttttaa tttacgctta ttctacgacc aagttataaa tagaccaact atgggagtgt
    34561 cgtttaaatc atcaaatata aattttggtg ttagtttaag atttactctt gcaacttaaa
    34621 aagattttta tttactttga aaaaaatagt attaacaatt aaaatttaac acaatgaata
    34681 ttccaaaaaa tttaaagtat gcagaatctc acgagtgggt acgcgttgaa ggtgatgtag
    34741 cagtagtagg aattactgat tttgctcaaa ctagcttggg cgatatagtt tttcttgata
    34801 tcgaagttga aggcgaaact cttgacaaag gagaagttta cggaagtata gaagctgtaa
    34861 aaactgtttc tgattcgtat atgcctgtta gtggcgaagt tatcgaagtt aatcaagaag
    34921 ctattgacga tccaagtatt gttaataaag atccttatgg agaaggctgg ttgctaaaaa
    34981 ttaaaatgag cgaccctagc gaagttaata gcctattaga tgctgctgga tacgaagaaa
    35041 ttgctaaaga ttaaataata caggctaact cgaaataatg atggtgcctt agaagtgttc
    35101 aattttaaca ctttatgaac tttataaact ttaagaactt tataaactca taatagttga
    35161 acacttttga gacaccgttt ttttaacata attttatatg gtttatgcta taattttatc
    35221 tggaggaata ggaacaagat ttggaaatca actgcctaaa caattcttaa agataaataa
    35281 taaaaccata ttagaatata gcatagaaac ttttgaaaca cacaacttaa taaataaaat
    35341 tattatagtt agcaatccca attttgttga agaaactaaa gaaattcttg ctaaaaataa
    35401 ttataaaaaa gtttgtgcta tcatttctgg cggaaacacg agaggagaat ctgcatatcg
    35461 aggattaaaa caaataaacg aaactgaaaa tgttaaggat aaaatcaaag ttctaattca
    35521 cgattctgta agaccaaaca catctaacaa aataatttct gaaattataa aaggttttga
    35581 tgagtttaaa gcacttagtc cggcaataaa aactacagat acaatttaca taagcaatgc
    35641 tacacaaatt ataaaatcaa ttcctaatag agacaatatt tacaaagcac aaacgcctca
    35701 aggtttcgat ttcaacacaa ttttcaacgc atataacaaa ttagacgact ctcaaaaatt
    35761 tgcttttcct gatgattgtt ctgtactaaa ctttgcttat cctaatatag aaataaaaat
    35821 tgttgaagga gacaacaaaa atttaaaaat taccttcccc gaagatttag aagtgtttaa
    35881 gcaactttta acacaataaa agtataaaaa agctgaaaaa tttcattttt tttcattttt
    35941 ccatgcaacg aaatgatgaa aattaatgtc ttatgttcag ttattatttg ttaaaaatta
    36001 attatatttg tataattgta aaaaacattg ttatgaaaaa gtcagtatta aaaatagttt
    36061 atttgcttat agttcttata aactcagcct tatatgttaa tgcacaatgc acaactaatg
    36121 ctggtaacat gaatcaaacc gagccagttt atctttgcga caatcagtgt agcgaagttg
    36181 ggcatagtgg tgctctttag atggagatga tattttagag tttgtattat ttgatccgaa
    36241 taattcttct caagtcttag caaggaaatc aacaccagat ttttgttttt cagacattcc
    36301 tgatggtcaa tacggtatta cttatcatat tgctgctaga gcaagaaata atctaggtgg
    36361 cattgttgat cctgacgccc catgcatgca tcaatcaaat ccatttgcta ttacttggtt
    36421 tagtacacca actgctcaaa tttcacaaac tcaaacaagc ttttgtggac taacaggttt
    36481 tttaaatgct acgccaccgc aatctggatt aagtggctat tggagttcaa atgtggaatt
    36541 tgttccaatc aatggtggta atgttaacga tgcaaataca gatattatgg taagtcaacc
    36601 aggcacacac acttttactt ggactgtaac aaatggaact tgttctgatg tagcatctgt
    36661 ggaaataaca tttgtgcaag cacctaatcc aatagtaaca aacgcttacg atacagtttg
    36721 tggaaattca tataatttaa acgtaatcac tactccagat gctgatgggc aatggagttc
    36781 atatatctgg aatgatgaag agaataattg ggtaatacca actactccac catatatatc
    36841 acttgttaca tctcctaaca ctacagtaaa tattgcgaat tatcctggtg cgacttgtcg
    36901 ttatcgcttt gaatggacag aaactaatac tactggaggt ataatttgcc aagggacagc
    36961 ttctaaagag gtagtttttg caaaaacacc aatggctagt gttggacttg taagtgaagc
    37021 agaactttgc ggaaactcct tccaattaga tgcagacaca tcaggttata gctgggcaac
    37081 tggaaaatgg ataagctcta atattgcaaa tccatttgac gatcctaact tgcctaatgc
    37141 tacagtaacc atctctaatc ctgaaaattt tggcgattct gcttatgttc aatttccttt
    37201 tgtttggact atgactaaca caatctgcat taccacagat actatgtggg taactttcta
    37261 tcaacgccct caagcaaatg cagaattaga tgatgctgtt tgtggatatg aatataattt
    37321 acatgctcag tggagccttg ctccaagccc taactatact cctagcgggg tttggcaaat
    37381 ggttccacaa ccaagcacag cgacagcaga tatacaagac atatacaata ataactctca
    37441 tgtaactgta agtgaaacag gagtttacca gtttgtattt agagaaaaca atactaatct
    37501 aacaagttgc tacactactg ataccgttca aattgagttt gttgaaaacc caattgctta
    37561 tgcgggagaa gatcaagatg tttgtggtta ttgtgctact ttgggagcaa taagcggagg
    37621 ttttcctggt gcatggttgc caaatggtac ttcttacgaa gatcatacag atccaacaac
    37681 tcaagtatgc acatcttatt atggaacaat agtatatact tggaaagcat ctaacacagc
    37741 tattactaat aactctttca catgtgttgc tacagatgat gtaagtataa ctttctggaa
    37801 aaagccaact cctaatatat taacagaccc agcagactca actgcctgtg gattaaccta
    37861 ttataaatta agagcagaat ttccaggcat aggcattaca ggctattggt attgtagtac
    37921 aactggtgtt gtttttgaga attcaaacta tactgaaact tctgtaacag ttcctcacta
    37981 cggatactac gatttttact ggatataaga aactggacca gaattatctc caggattttg
    38041 caccgacacc gcaggacctt atactgttca ttttatggaa gcacctctag caaatgcagg
    38101 tggagacact tatttctgtg gtctaacagg acaacttaat gctatcccaa gtataggaat
    38161 aggcacatgg tcaacaagta gtactgagta tataaatttt gaagacacta atgacccaaa
    38221 caccacaatt acatctacaa ttataaatac tgaaaactca gaccaacctt actttgaggt
    38281 aatttggaca gaaaactaca caaatggctg tacagatgca gataccatta aagttgtatt
    38341 tgcaactgca tcaaattctt attcagaaga acacagcatt tgtgaaggcg atatttattc
    38401 ttggaatgaa caagattata ctgaaacagg aacttataca gcagaattta ctaatgttta
    38461 tggttgcgat agcattatca cattaaatct aactgttaac cctcttcctg aacaaattgt
    38521 agtatcacaa aatccagaaa atggaattct gtctgaaggt acaagtggta gtattacgct
    38581 agaaaatagc ataatcgaaa ctaattattg ggttagtatg ggaggcgagc cttttactga
    38641 agaaattact ggtacaggtt ctgaattaag tttaggtaat aattttacag atggaagtta
    38701 tgatattaga agtcgtaatc aatttgattg ttctttagtt caaggcattg ctaattttgt
    38761 aacagaagta agtggaacaa acaaaattgt tgctaacgta agttttggta cacctgcaag
    38821 taattttcct gctaatcacg taaatgttaa attatataaa gcaacaacaa ataacgaaac
    38881 catagtttta gtggccgaac aattattaag tagcaatgga caagttgagt ttactaattt
    38941 agaaactggc gattattatt taggttcatt tattcaatat cctgaaaatt ataatgtggc
    39001 agaacatgtt tattatcaaa atgttgttgt tcacgaaaat gctacaagca ttccaatggc
    39061 tgaagaaaca gtatatatag ccaacataca tcatattgca ctagcagaaa gccaaggctc
    39121 aaatgtaatg tatggtattg ttggaactat gaatagcaat gaaagtttaa atccattaaa
    39181 tgaaatggtt gttgttttaa gaaatagtga tattaacgaa tttattggtg taaaagttac
    39241 tggcgacaat ggtcaatatc actttggcaa tattcctgaa agtactaata tacaagcgtt
    39301 tgttaccagc tttatacatc caaattggac tgcttttgaa attgaaacta ctaacgacca
    39361 aaattataat gttaacttta ttgttgaagg cacaagtgtt tatcctgatt atattacaat
    39421 gcttgaagat atactaattc aaaatattga atttactatt ttcccaaatc ctgcaaaaga
    39481 agtattatat ataagttgcg atattgaaaa agctgtttat caaatttttg acataaacgg
    39541 cagacttgta aattcaggtt tagctttctc aaatagcgaa ataaatattt ctgatttagt
    39601 ttcaggaact taccttatta ttttatcaac agataatggt aaaactggaa tacaaaaatt
    39661 tgtaaaacaa taattatacg tagaaactgt ctcaaaacaa attttagaca atgaactttc
    39721 aaggagtttc agaagtgtta aaatgcaagg cgctgaaatt gaggacgaag gagcgtacac
    39781 ttgagttaaa agttaaaagg ttaaagttaa aagtgtcgcg tcctaataaa agttgacaca
    39841 ttttgttata aaaccgactt taaaaacttt atgaactttt aactttat
//
LOCUS       DAOPNX010000012        39638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_1338_length_39638_cov_7.805472, whole genome shotgun sequence.
ACCESSION   DAOPNX010000012 DAOPNX010000000
VERSION     DAOPNX010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 39638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39638
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1338_length_39638_cov_7.805472"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PKN53_02390"
     CDS             complement(<1..686)
                     /locus_tag="PKN53_02390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HOD87834.1"
                     /translation="MKAKHKEIFYRLRLHRLVPSNIFLFIGHLAKLSRFVNKHKNDGY
                     SDYYSPKYDFNKKKKLYLHVIETQKLNEIPIDFFEFGVYKGVSFKWWVQHITHKDSRF
                     YGFDTFTGLPEDWGTFKAGHFAIEDNAIPKIDDTRASFYQGLFQQSLLPFLKNYNPEN
                     RKVIHLDADLYSSTLFILTTLYPYLKSGDILFFDEFSVPMHEFKAFTEWTNAFYVEYE
                     VLGSVNNFFRL"
     gene            810..1685
                     /gene="era"
                     /locus_tag="PKN53_02395"
     CDS             810..1685
                     /gene="era"
                     /locus_tag="PKN53_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOD87835.1"
                     /translation="MHKSGFVNIIGNPNVGKSTIMNELVGEKISIITEKSQTTRHRIL
                     GIVSGDNFQIVYSDTPGIIKPKYKLQEKMMDFVTTSFEDADIFLYVTDVIENPKKNIE
                     YIEKLSKVNVPVVCLINKIDLADQQKVEELQEFWKENLPNAEIYAISALEKFNIEGLF
                     DRIIELLPEGPAWFPKDQLTDKTERFIVSEIVREKIFKNYQKEIPYSCEVVVEEFKEE
                     ETIIRIKVNIIVERQSQKGIIIGHKGASIKKVGTQARIDIESFFEKKVFIEFFVKVKK
                     DWRDNDKSLRDFGYV"
     gene            complement(1812..6200)
                     /locus_tag="PKN53_02400"
     CDS             complement(1812..6200)
                     /locus_tag="PKN53_02400"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOD87836.1"
                     /translation="MKRLITIFGLTIFGLSKLFAQSTTGTDFWLSFMQNFNTPVSTQI
                     YITSDVGATGTVSIPGSGWSVNFTIGANGSQYIEVPAAQNACISQPNTVLNRAVRVQS
                     DTPVAVYAANQRTASSDATIVFPTIALGDNYLVTTYSHFSSQPSEFIVVGIEDGTSIE
                     ITPKANLSGGGGNANIPFSITLNAGEVYLVQSLGDLTGSTVKSTELGNCHNFAVFAGN
                     TCANVPLSCTYCDHLYEQMIPVKAWGNNYITVPLMTRASDTYRILACEDATIVNINGG
                     ANINLNAGQFHEVNLSTTSFITSDKPISVAQYSQGTSCDGVTSDPFMIMLSPIEQTLD
                     YIVFQAFNTTAINQFYTNIVAKTSTTSEVLLDGSPLTGWATVPANNAYSYVRKNLTQG
                     SHYIISPEGVLATVYGFGDVESYGYLAGANIVPLNVSFDIVVDGTSTAFDVFQDTLMC
                     TQTTVSFQATSPDITNVEWDLGDGSPIVVGNQVINHEYPAVTDTYTVTMYFLREGACV
                     QEHISMEVNINSNLPPITELNDTIVCNGDPFTITLNDPGITYQWQDNSISNSYTFANT
                     GTYSVTATDAVGCSASSTYFVEFINLSVGILTQDVTCNNMNDGEISANVFGGHQPYQY
                     IWNTNPQQTTQSLTNLEPGEYSVTVTENSGCSATATGTIANQPSLVVNVTNIQGILCY
                     GDSNGTARVNVVGGTEPYTIAWNPDNITGFEQTTLVAGDYQFTVTDNNGCTGEAEFTI
                     NDVTPLSYVANTQDVDCYGNQTGRIEFNASGGTGSLTYNWSNGLMTSSLNNIVAGEYI
                     VTVTDYNNCSLSAEFEITQPDELELHIYPTNIDCYGNNNGSILLNVVGGTTPYSFNWN
                     SGQTSQNLENLIFGTYIVTVGDANGCSSFGYAILEQPQLPLHGNITPTHVRCFGEGNG
                     VADLTVVGGTEPYYYSWNTGAISEDIDNLIPGTYMVTITDHNNCTHADTVQIIQPSAP
                     LSGTISGTHVSCQGNTNDGNVYITAQGGRPPYHFEWSNGSWEQNLIGVGVGIYSVTIS
                     DQSGCHHVMEYEITEPEPFYLQAMDDQVICYGMTTEIGIGIITGGVAPYTIVWDNDDQ
                     GMTTLVSPTETTTYSAHVVDAGFCVSQDIEITVYVHKPLELNVSVSNNIVCPGSQVTC
                     TATVSGGGLTNNNVWVNDSLMSNPIRLTIYNDTVFRFRVQDVCGFAEVTKDIHISTYE
                     LPLINPRADRYSGCSPLEVNFEETSPDQGQRYVWNFDDGDFENLSFAKNPTHIFENAR
                     TYSVNLTIFSKNNCAVDSTIGITVFPVPEVDFRADRESVSTEFPLVNFSNYSVGAFWY
                     HWDFGDGNTSKDRNPSHTYSAPGTYTVTLEAESLYSCSDTAQKIIVVNTEHTIYAPTA
                     FTPNYDGMNDKFRVFVANANTKEWRFIVYNRWGEIVFNTEDYEQEWDGRYNDMECPVG
                     VYTWYVQYKDLYGNDYSETGYFTLIR"
     gene            6468..8243
                     /locus_tag="PKN53_02405"
     CDS             6468..8243
                     /locus_tag="PKN53_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOD87837.1"
                     /translation="MEKLKSGEFLVKSTNSSDIFIPEEYTEEQLMIAHMIQDFLDAEV
                     NPIVAKLDEGNRELMAATIRKAGELGLMGIAIPEEYGGFEQDFVTQMLAAENIGAGYS
                     FSVAFMASTGIGTLPIMYYGNEEQRQKYVTKLASGEFIGAYCLTEPDAGSDANSGKTQ
                     AVLSEDGKHYILNGQKMWITNAGFADTHVVFAKIANDRVLSAFIVEKTMPGVEIGPDE
                     KKMGIKGSSTCQIYYNDVKVPVENLIGRGGMGFRIALNILHMGRIKLGANVIGAAKKS
                     ISDAVKYANERKQFNVLISTFPTIKHKIAEQAIRTFALESAIYRASHDIDCLMKKYKA
                     EGMDKGLAAIEAIAHYAVEAALLKVYGSEVLDFVVDETVQIHGGMGYSAEMDADRGYR
                     DSRINRIFEGTNEINRLLLVETAIKRGMKGDFDMAGPAKEFYENMENCRINKKDGENF
                     FEQKLRYIENFKRAALILIHKVSDKYEKDMLREQEIINNISDMMIQIYTAESLTLRVK
                     KIAETNAKPNLELYKDIVNVLVYEAAAVISKNAKDAVFSMTEGEESEKMYKALKDLSC
                     VEGVNTKDARRRIADFLIDKGEYKL"
     gene            complement(8326..9645)
                     /locus_tag="PKN53_02410"
     CDS             complement(8326..9645)
                     /locus_tag="PKN53_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOD87838.1"
                     /translation="MTLLSATNVNMPLWAIIPFVLMLLCIALGPLFFEHWWEKNRNKL
                     LVSIILGIPVAGYLIALGLPELLAHQLIFDYLPFIILLGALFVITGGIHLKGNIQAKP
                     WINTSFLGIGAILASIMGTTGAAMLLIRPVIRTNNARKFKVHTILFFIAIVANAGGLL
                     TPLGDPPLFMLYLKGAEFTWFFHMLPEWALVNALLLLIYFIADTYYYKKEPLENIKFD
                     KTNVEPLGLSGNINFIFLAGVVASVAFLNKQYLPIMAESDWAGFIREGSIVSMAVLSL
                     LFTKKPIREANKFSWGPILEVAFLFLGIFLTMIPALRYLSENSNSLGISSNAQFYYAT
                     GSLSAFLDNAPTALAFYNLALGMNDGSVVEGVRYVAGVDEMILTAICLGAVLFGAMTY
                     IGNGPNFMVKAIAEESKIKMPSFFGYMIKFSLIVLLPVYILVQLIFL"
     gene            complement(10266..11582)
                     /locus_tag="PKN53_02415"
     CDS             complement(10266..11582)
                     /locus_tag="PKN53_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOD87839.1"
                     /translation="MILLSATKSMPLWAIIPFVLMLLCIALGPLFFEHWWEKNKNKLL
                     VSLILGIPVAGYLIAQGLTGDLKHQMLFDYVPFIILLGALFVITGGIHLKGDIQAKPW
                     INTSFLGIGAILASFMGTTGAAMLLIRPVIRTNSERKFKVHTILFFIAIVANAGGLLT
                     PLGDPPLFLLYLKGAEFSWFFHMVPEWALVNALLLLIYFIADTYYYKKEPLENIKFDK
                     TNVEPLGLSGNINFIFLAGVVASVAFLNKQYLPIMAESDWAGFIREGSIVSMAVLSLL
                     FTKKPIREANKFSWGPILEVAFLFLGIFLTMIPALRYLSENSNSLGISSNAQFYYATG
                     SLSAFLDNAPTALAFYNLALGMNDGSVVEGVRYVAGVDEMILTAICLGAVLFGAMTYI
                     GNGPNFMVKAIAEESKIKMPSFFGYMIKFSLIVLLPVYILVQLIFL"
     gene            complement(11688..12356)
                     /locus_tag="PKN53_02420"
     CDS             complement(11688..12356)
                     /locus_tag="PKN53_02420"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008647922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HOD87840.1"
                     /translation="MRKKSLIAIAIILGILILDQVLKIWIKTNFTLGQELNIIGDKIK
                     LHFVENEGMAFGMTFGGSWGKLFLSLFRVVAISLLIYYLCKIIKAGENLFYIICISLI
                     IAGATGNLIDSAFYGLIFNESTYYQVATMFPPEGGYAPFLHGKVVDMFYCPIIETTYP
                     TWFPFVGGKEFLFFRPVFNVADSAITVSVFLLLIFYKRIFGESILESLKLKKKSSNSE
                     TSEQ"
     gene            12532..12699
                     /locus_tag="PKN53_02425"
     CDS             12532..12699
                     /locus_tag="PKN53_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOD87841.1"
                     /translation="MAYKITDDCAACGTCIDECPVEAITEGAIYVINPDLCIDCGACA
                     DACPVEAIKPA"
     gene            complement(13073..14290)
                     /locus_tag="PKN53_02430"
     CDS             complement(13073..14290)
                     /locus_tag="PKN53_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOD87842.1"
                     /translation="MTEKQKTNVFIPGSVYLPQEEMLELVQDKKKFRIGMPADKTGNE
                     RRVPLAPLAVEVLVNEGFEVVIQDGAGKGAYFSNHEYSKYGAEITTNLADVFSCDIIV
                     KICPLTIEEMVLLRGNQIIITSLHSTYQTKEYFQILMKKKVTALAFDMMRDSKGANPI
                     VRSMSEIAGRSAVLIASEYLNNIHKGKGEMLGGITGVSPSEIVVIGAGIAGTFATQTA
                     IALGAHVKVFDNSIAKLDKLQSLIGTKVFTSTIYSKVLLAAIKTADVVIGAVRINNNR
                     PNIIVTEDMVMQMKKQSIIVDISIDQGGIFETSKPTTHKSPVFTKHGVIHYCVPNIAS
                     RVARTASYSISNILSFELLKIQTAGSINSYLKTNAGVRYGVYIYDGILTDENIGQIHG
                     LMAKDINLLLAAM"
     gene            complement(14335..16497)
                     /gene="rnr"
                     /locus_tag="PKN53_02435"
     CDS             complement(14335..16497)
                     /gene="rnr"
                     /locus_tag="PKN53_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOD87843.1"
                     /translation="MKKTQKKNSKQPETNSLNKKTLTSQILNIFTKSPQKELNYKQIS
                     SMLSINDSHNRDLVIKALDELVGIGNLKIVSKGKYKLKSKSSHIIGTVDLKKSGKAFL
                     VSDELSEDVFIPFTKLRSALNGDKVKVCLFALRRGGKMEGEVVEILERANTRFVGVIE
                     KSSTYAFLIPDNQKMPLDIFIPNNYLNNAKEGDKVVVDVVEWHRKGKNPTGKVVEILG
                     RPGENEVEMHAILAEFGLPHKFPDAINKAAEKISDKITQYDLETREDFRSHTTFTIDP
                     EDAKDFDDALSIEFLDENLYNIGIHIADVTNYIKENSELDNEAFSRATSVYLVDRVVP
                     MLPERISNYLCSLRPNEDKLCFSVIFKMNDKAEVLDYRIVKTIINSDRRFTYEEAQKI
                     IDTGEGDFAKEIQTFNNLAKILRKERFKKGAFNFEHSEVKFILGKNAVPTGVYFKEMT
                     DSNHLIEEFMLLANRKAAEIYGKIKSPKPMVYRVHAEPNMEKLMNFSNFISRFGYTID
                     QKNKISLAKSINNIVKDVNGKAEQNIIENLAIRAMAKAVYTTKNIGHYGLAFDYYTHF
                     TSPIRRYPDIMVHRLIFDYLRKNKPDTEELEQNCKHCSYQEQQASLAERASIKYKQVE
                     FMQDKVGQVFDGIISGVTEWGFFVELKENACEGLVHIRTLTDDFYEFNAEEYCIVGSY
                     TGKVFQLGNETKVKLVNVNLQKKQIDFELDYSEIVKEV"
     gene            16618..17202
                     /locus_tag="PKN53_02440"
     CDS             16618..17202
                     /locus_tag="PKN53_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87844.1"
                     /translation="MRTVTLMKSILVIAIISTAMVFSSCKKNTKPGGKKAVEIEIPCK
                     DEGRSDKNFFRADANATSQDMSLSREKALLAAKQRVSSLINSKIKSVTDRYVNETEVN
                     QNSDFEAKFENLTREVVNQTLVDVAITCEKNFQEATGKYTTYIAVEVSKDAMINGIEK
                     GLSKDQKLRVDYDKMKFEQIFNEEMDKLSKEGGY"
     gene            complement(17632..18228)
                     /locus_tag="PKN53_02445"
     CDS             complement(17632..18228)
                     /locus_tag="PKN53_02445"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOD87845.1"
                     /translation="MLKQYYLEGQIEAGCDEAGRGCLAGPVYAAAVILPPDYKNEILN
                     DSKQLSSKTREQLRIEIEKNAIAWAIGFCTNEEIDKINILNASYLSMHRAIEKLKQKP
                     EFLLIDGNRFRPYPEIKHKCIVKGDATYLSIAAASILAKTHRDQFMLNVHEEFPNYDW
                     NKNMGYPTKKHRQAILEFGITKYHRKSYKLLDKQLKLF"
     gene            complement(18221..19360)
                     /locus_tag="PKN53_02450"
     CDS             complement(18221..19360)
                     /locus_tag="PKN53_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOD87846.1"
                     /translation="MNIAVNTRLLLKNKLEGIGWFSYETLKHIVKTHPEHNFYFIFDR
                     NYDPSFIFADNVKPIVAGPATRHPFLWWFWLKFVIPRVLKKVNADIFLSTDGFVSLNT
                     KIPQAVVIHDINFYHNPQQLPFWVRKFYNKFFPKFAKKAKHIGTVSEYSKKDLINVFK
                     IAPEKITVCYNTSNDIYKPITESEKIKVKEKYTDKQEFFHFVGALTPRKNIPGLLKSF
                     DFFKENSKLPHKLVIVGSAMHLTGEIDKTYKEMKFSKDVVFTGRLNIDELHLIYASST
                     ALVYIPFFEGFGIPLVEAMYSEIPIISGNTTSLPEVVGNAAIICDPNNHKEVAQHMEN
                     IALNSELRQDLIEKGKIQRQKFSWEKSGERLWECVEKAIQASNHA"
     gene            complement(19360..20109)
                     /gene="kdsB"
                     /locus_tag="PKN53_02455"
     CDS             complement(19360..20109)
                     /gene="kdsB"
                     /locus_tag="PKN53_02455"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOD87847.1"
                     /translation="MEFIAIIPARFGSTRFPGKPLVQINNKSMVHNVYVQASLVFDEV
                     WVATDDVRIVNEVESFGGNVVLTKKKHKSGTDRCAEAIEKIQTLKNKKWDVVINVQGD
                     EPFIKTEQLKEIKKCFKSKRTQIATLIKPINNKEDIFNTNKPKVVINNKNEAIYFSRL
                     PIPFLRGVHETRWVTKHSFYKHIGLYGYRTDILLEITKLEVSKLEKAESLEQLRWIEN
                     GYKIKTAFTEHESISIDTPKDLEKVKQLGLL"
     gene            20187..21263
                     /locus_tag="PKN53_02460"
     CDS             20187..21263
                     /locus_tag="PKN53_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOD87848.1"
                     /translation="MENKNTDTSRFFKWWKIAIPIVLGLGVVLYFVLRDFNQIDFSLF
                     KFTKYTLLFLILAFVMLVFRDFGYVLRIRVLSGGELSWMQSLKIVLLWEFGSAITPSA
                     VGGTAIATIFLWKEGMSVGKSSAIVLATSFLDELYFSLMFPLILLIVGKSQIFVGEQN
                     VFLEKLFWFAIAGYCLKLAWTILIGYSLFVSPKFISNFLKKIFRIRFLKKWRASAEKV
                     ADDYVLANKEFKNKSFVFWIKSFFATFISWTARYLVLNALFIALAVGNYTLSFYDHIL
                     IFARQLIMWIIMLVMPSPGGSGFVEGVFSSLMSDFVGIAGFAIIMALVWRLFTYYPYL
                     IIGAVLAPHWLSKKFKRTKKEAVS"
     gene            complement(21311..22978)
                     /locus_tag="PKN53_02465"
     CDS             complement(21311..22978)
                     /locus_tag="PKN53_02465"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOD87849.1"
                     /translation="MKDIDIAKAAKLELIDEIAAKLGISKDDIEHYGKYKAKLPLHLI
                     DEEKIEKSKLILVTAITPTPAGEGKTTTSIGLAQGMNKIGHKTTVVLREPSLGPVFGV
                     KGGATGGGYAQVVPMEDINLHFTGDLGAIEKANNLLAALIDNNIQSKTRNLGIDPRTI
                     QWKRVMDMNERALRKIVIGLGGQTQGVPRETGFDITAASEIMAILCLSKDLEDLKEKI
                     GNIFVGYTYDKKPIYARDLNAQGAVATLLKDAIKPNLVQTLENTPAIIHGGPFANIAQ
                     GTNSLIATKMGLSLSDYVITEAGFASELGAEKFLDIKARYGGLKPDAVVIVATIRALK
                     YHGGVGLKELANPNPEAVKIGLENLDKHIENVQHFKIQPIVALNQFVTDSKAELEVLE
                     NHLKAKNVKYALNSSWAHGGDGALDFAKVVVETVNAGKSNFEPLYELDWSMEKKIETI
                     ATKLYGASGVEFTAKAKKDLRTIDELGLSKLPVCIAKTQNSLSDDKNLRNRPKDFIVN
                     IREIEIASGAGFVVPISGEIMRMPGLPETPAAEAIDIDNEGNIVGLF"
     gene            23160..24101
                     /locus_tag="PKN53_02470"
     CDS             23160..24101
                     /locus_tag="PKN53_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOD87850.1"
                     /translation="MKKILVTGGAGFIGSHLCERLLNEGNEVVCLDNYFTGTKANVIH
                     LLDNPYFELIRHDITMPFYIEVDEIYNLACPASPIHYQYNPIKTIKTSVMGSINMLGL
                     AKRIKAKILQASTSEVYGDPEIHPQPETYWGNVNPIGSRSCYDEGKRCAESLFVNYHK
                     QNNVKIKLIRIFNTYGPRMHPNDGRVVSNFIVQALTNKPITIYGDGSQTRSFQYVDDL
                     IEAMIRMMNTKDEVIGPINIGNPGEFTILELAEKIIKLTGSKSELEFYPLPEDDPIQR
                     KPDISLAKKHLNNWEPKIDLEQGLVKTIEYFKKTIKL"
     gene            24098..24973
                     /gene="rfbA"
                     /locus_tag="PKN53_02475"
     CDS             24098..24973
                     /gene="rfbA"
                     /locus_tag="PKN53_02475"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOD87851.1"
                     /translation="MKGIILAGGAGTRLHPMTISISKQIIPVYDKPMIYYPLSTLMLA
                     GIKEVLIISTPTDIGSYERLFGDGSQLGMKISYKIQPSPDGLAQAFVLGEEFIGDDSV
                     CLVLGDNIFYGQGFGSVLLESAKLEDGALIFGYYVNDPQRYGVAEFDKDGKVISIEEK
                     PQNPKSHYAVTGLYFYSNDVVQKAKTLKPSKRGEYEITDLNNVFLSENRLKIKLLGRG
                     FAWLDTGTPESLLQASNFMETLEARQGLKASCIEEIAYRRGFIDKKQLEKLIEPLSKS
                     QYGQYLKRILNEDIL"
     gene            24970..25518
                     /gene="rfbC"
                     /locus_tag="PKN53_02480"
     CDS             24970..25518
                     /gene="rfbC"
                     /locus_tag="PKN53_02480"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOD87852.1"
                     /translation="MKVVKTNIEGLLVLEPKVFTDSRGYFLESWNNKVFLENNLDFKP
                     VQQNESKSSYGVVRGLHYQLAPYSQAKLVRVISGKVLDVAVDLRKNSPSFGKYYSVIL
                     SEENKKQFFIPKGFAHGFSVLSDFAIFSYMCDEYYSPEHERAISYNDNFLNIDWQVPF
                     DKMIISNKDMAAPCFKTAEYNF"
     gene            25520..26383
                     /gene="rfbD"
                     /locus_tag="PKN53_02485"
     CDS             25520..26383
                     /gene="rfbD"
                     /locus_tag="PKN53_02485"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOD87853.1"
                     /translation="MKILVTGSKGQLGSKIKDLSVDFQSFSFSFTDLDELDITNFNSV
                     LDFFKFEKPDIVINCAAYTAVDKAEDDYENAFKINALGPENLAKACNVHNSKFIHIST
                     DYVFDGKEREKPYLESDFTSPQSVYGSTKLEGENLVLKNLQDAIIVRTAWLYSEYGHN
                     FVKTMLNLAQSRTELSVVNDQVGSPTYAGDLAQAILQISMQYFEENYWKSGVYHFSNL
                     GKCTWFEFAKEIFKIKNINIKVNPVTSEEFPSKVKRPKYSVLDKEKIITTYKIDVPDW
                     RDSLKIVLEKL"
     gene            complement(26386..27255)
                     /locus_tag="PKN53_02490"
     CDS             complement(26386..27255)
                     /locus_tag="PKN53_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009162880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOD87854.1"
                     /translation="MQPIAESELIINKDGSIFHLHLKPEQLADTVLLVGDPGRVATIS
                     DMFDKIEHKVQNREFVTHTGTYKGKRFTALATGIGTDNIDIVLNELDALVNIDLKTRL
                     PKPEHKTLNLIRIGTSGGLQEDLDVDSFLMTETAIGFDGLLNFYKKRNSVCKLDIEKE
                     FKTHMNWNPLLTSPYFVDGSKELLEKLGDGIRKGMTISAPGFYGPQARVIRLDIQDPD
                     INDKIRSFSYDNKKITNYEMESSALFGLSALLGHKAATICVIIANRYAGKYSKDYKPA
                     VKKLIETVLNRLV"
     gene            27327..28142
                     /locus_tag="PKN53_02495"
     CDS             27327..28142
                     /locus_tag="PKN53_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOD87855.1"
                     /translation="MSKKRILVPTNFTSETEIAFEQSVLFSLKTDADIILFHVIPSLK
                     MINSNSSNIDRVQEELSILAEKYFKETGKNAQTRIEVGKVLQQILMLEKEIKPSYIFL
                     GSDVSKGEVSSLTLKLVNSVECPIIIFLRNFKKKGCEKIVLPLDLTKQTKQKVDHTIK
                     LAKIYNSEVHIVSATNFTNETKLAKLNDQLDYVNSIFEKLNIPTKTKILKTSNDIEIM
                     ANAINDYADDIDADLTVIMTRQETKIQKFFVGSMATKLINKSKIPILCISPNL"
     gene            complement(28217..29017)
                     /locus_tag="PKN53_02500"
     CDS             complement(28217..29017)
                     /locus_tag="PKN53_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87856.1"
                     /translation="MDAKKYLSLVLLGMIVILPLFTTSCFKKGDEDPFFSIYTRKARV
                     SGRWILSNYESTIKRTYQNKPDQTLTVTTIDGEDWSRNIEILGTDSVVDIKGKIVTGR
                     NTIQYYSDGRFTEILEYEYNVIEVDPITENENVTIYKVQDELSGTWNFLANIDDYKNK
                     ERLSLVIEQNKSKTFVYLLQLSEDDEATPIPQLINTVSSSRKYANGESSTIWTLRMLK
                     NKQIIQDQLVDRFVVETVNGVGDVYTEVGSVTRTLKAESTKAETSPQQ"
     gene            29314..29763
                     /locus_tag="PKN53_02505"
     CDS             29314..29763
                     /locus_tag="PKN53_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87857.1"
                     /translation="MVIDKKEISARLKLVIMLSFLVYAAILVMSLVLGWSKTHLFELC
                     WTIVFVVWFIFVFLKNYNYIWYSSDGPKIIVRYTSLQPLSSGKFSIEFQKKDFVKAEI
                     VKSVLGIRKNLVLYSRTPQGVAKYPKINISILNKEELKKIKEDLNID"
     gene            29795..30916
                     /gene="dprA"
                     /locus_tag="PKN53_02510"
     CDS             29795..30916
                     /gene="dprA"
                     /locus_tag="PKN53_02510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOD87858.1"
                     /translation="MTDSRIYDLAISFLDGIGDINAKKLISYFSSTKAIFDAKIKELS
                     SVAGIGEITAKTLYASFDKALQLAQEELEYIYTNEIDFVCFGDKNYPFRLKECDDAPM
                     VLYYKGLSDFNAQKIISIVGTRKATNYGIDFVETLTSSIASKYPEAVIVSGLAYGIDV
                     AAHKFALNCDLRTWAVLGHSLEFIYPASHKSVAKKILEQDGCILSDYPHGRKIDPAHF
                     AKRNRIVAGLCDALIVVESGERGGSMITANIANHYNRDVFALPGSINSSYSKGCNKLI
                     KTNRANLIESIEDLEYIMNWSDGKPSSLQQKLDLISDLNENEAKVVNILKKYEYLDID
                     NLLRQTNMDNNILSLCLLELEFKNIVRALPGKLFSLKIK"
     gene            30919..31689
                     /locus_tag="PKN53_02515"
     CDS             30919..31689
                     /locus_tag="PKN53_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOD87859.1"
                     /translation="MLIDTHCHLYLPEFEQDIDETIKRAEAQSVSKIILPNIDSESLN
                     PMFTLYNSNATLFSTTIGLHPCSVKKDYEEELSKIFKDYTNIINIIGIGEIGIDLYWD
                     KTFFKEQQKAFDFQLNYACENDLPVIIHSRNSLKEVLDILKIYLPKGLRGVFHCFPGN
                     LEEAKQIVDFGFYLGIGGILTYKNNNMLEVVENINLEYILTETDSPYLSPVPHRGKRN
                     EPAFIIEIVKYISKIKNIDFEKVASITTKNAKTLFKLQ"
     gene            31819..31912
                     /locus_tag="PKN53_02520"
     tRNA            31819..31912
                     /locus_tag="PKN53_02520"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31854..31856,aa:Ser,seq:gga)
     gene            31978..32799
                     /locus_tag="PKN53_02525"
     CDS             31978..32799
                     /locus_tag="PKN53_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOD87860.1"
                     /translation="MKKLIALLTLAGMLTFAGSSFIYAQDEAKTDENTTEQVEETVAE
                     EVAVIDEGDAAEASGENVPMHQQLKVKFIEGGPMWMAPLIICLILGLALAFERIIYLN
                     FASTNTKKLLLKVEEALNEGGIEAAKDVCRNTRGPVASIFYQGLDRHEGGIDIVEKSI
                     ISYGGVQMGLLEKNLTWISLFIAIAPMLGFLGTVVGMIMAFDEIQIAGDINPSLVAGG
                     MKVALITTVAGLIVAMILQIFYNYILGKIEGIVNSMEDASISLIDILVEYEAKQK"
     gene            32818..33327
                     /locus_tag="PKN53_02530"
     CDS             32818..33327
                     /locus_tag="PKN53_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87861.1"
                     /translation="MLEKVSKIFGITLLVLVALVTLHFFIFDLGALETQIAETADLPS
                     DLKAFKMQEIGINWGATILYFAIVLFALAAIFLVGALIWQVVRTIIDDKSKAPVILIT
                     LAVIVGIVLLCYFVFASSEVPFFLGSDKIEVTGETCRLIDTGLYVLYITLGLTICAAI
                     YSEIAKFWK"
     gene            33357..33938
                     /locus_tag="PKN53_02535"
     CDS             33357..33938
                     /locus_tag="PKN53_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOD87862.1"
                     /translation="MAKRETPEVNSGSLADIAFLLLIYWLLTTTMDVDTGIARQLPPI
                     PEKSDITEVDINKRNIFIVLINSENVLRVNGKERKIEELRELTKEFVKSNPDDPSMPE
                     LESVYIEQLNKRVDVGKGVISLQNDRGTSYGTYIMVQNELVAAVNELRDEAAYEYFGK
                     KSFDNLSDDQKEAVRKIVPQRISEAEPKNIGGK"
     gene            33941..34414
                     /locus_tag="PKN53_02540"
     CDS             33941..34414
                     /locus_tag="PKN53_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOD87863.1"
                     /translation="MAKFSGKKKRKVGAINTSSLPDVVFMILFFFMATTTMREVELRV
                     QVKSSDLPKATEIKKLQKKSLVSYIYVGKPLKEYVGMYGTEPRIQLNSSFAELSDIYE
                     YILAEREMRDEAERPFMTTSLKVHQDTKMGIVTDIKQELRKASALKINYSARKEI"
     gene            34549..34974
                     /locus_tag="PKN53_02545"
     CDS             34549..34974
                     /locus_tag="PKN53_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87864.1"
                     /translation="MRKIILISFVMLCFGFSKAQTSTTPDPRLLDVYSQEYLDANLEN
                     EIEYFNWFLDNSYFVAFVGLEKAETLPYLYEIDKETKEKKNKILAIDEESFNPLLSSY
                     EIFYNKESAYRIGNTGYVIAFYSQKKLTKNYNQYKNENQ"
     gene            34976..39520
                     /locus_tag="PKN53_02550"
     CDS             34976..39520
                     /locus_tag="PKN53_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOD87865.1"
                     /translation="MKIFIISIFLLIGATSFGQQYLISQGGTITTCEGTLYDSGGPNG
                     NYSSSENYTITICSEDGNFLVLNFTQFEFESGTYDKLSIYDGPNTTSPALVTNAGGTT
                     LLNQTFTSTGSCLTLHMQTDGSITRPGFAAVISCLPPCQDFEIDIISTSLPFSQPLDS
                     MWINVCLGESITFTAAGTYPNNNVNYTQSDATTDWYWTLSSSGPPIEANGVGMTTFTH
                     TFNESGGYFLGLVGKDANGCLVSYLEQIRVRVSIPPNLSVDPAVGGCISDAIELSGHV
                     TTNSWEIVIADEQIVQECITDDQGVVQSFCWNINAFQPGQTITSLNDLEHVCLNIEHS
                     WVADVWIYIECPNGQQAEINYYSTSNPCSGEFFGSPNHSDNCVPGTGWDYCWTMNATQ
                     SHTAWCQGNSGSVPPGDYLPTGNFADLIGCPINGEWCVIIIDDWGSDDGTLFSVDIGF
                     NQAVTPSYLWSYSHTYAPTGMSWSGNGLGANSGGTATAFPTESGTQAYNFCVTDDFGC
                     SYCIPTTAEVLSIDDPSCCILPSTNAGLDAAVCSNTYTFNASIAAGNTGTWNMISGPG
                     TATWANQNSPHATVTVSEWGVYEFEWIEVYMNNPTCADTATVTIEFWPIPTTTFTYDQ
                     VMCNADHTTINYIGNMSATANFNWDFDGATVVSGSGIGPYTIYWAEPGMHYVGLTVSE
                     NGCSSPDTLVNILNPALLEHSLVVDNDPCFASCRGRAEVFPTGGTEPYEYSWGSHNPV
                     LPNLCAGTYSIVVTDANGCTTGQNFTVGEPPELVINNITYNHLTCYQSNDGNISIDAV
                     GGTGELTYQWSDIGFGPANRENISAQEYCVTVTDANGCSLTDCINITQPAELLVTVSP
                     NVAICEGSQTVLQATGMGGTVPYSYMWRYSQDSAYIVGPSSYMVSPDTTRIYEVYVRD
                     AHGCESNVASIKVTVSPEMIIDTMLIKHNRCYQSCDGRAEIVMHGGLAPLHYSWGSDN
                     HIYSGLCAGIYTVTVSDLIGCSVSNTFVIEQPTQITYSTSVEPATCFGYDDGEATIFV
                     QGGVTPYTYTWSTGPGTETITTTAGSYTVTVLDDHNCRIVANVEITQPSKIIVLPMSN
                     MRICQTQSVSLNNQATGGTPYYDFRWEGSDGSVWNSNIYTVTPETTTTYTLVVTDSHG
                     CHSSPVSTTIFVNPGLTINSIVTSNDTVCPGDPAIIHVDVEGGNGGPYLMTLQDGRVV
                     PSPFIVYPDTTTMYYITLADMCGTPTVMDSILINARPKPVNLFTVEKVSGCPPFAAQF
                     NELNPHLGQTYLWNFGDKKYSTEKNPVHIYKEPGVYTVSLEVRDVFGCKFTREIEDLI
                     TVYQGPTANFEASPAIVSFINSEVEFINHSSNASNYYWFFGDGDSSMFVSPRHKYTDI
                     GDYEVMLVAETDKYCTDTTVRTIGVRNEFAFYMPNSFTPNGDGLNDCARPCGNGIDKN
                     DFSLVIYDRFGMLVFETDKFDPKVGCEACSDGAWDGTYKGNKNKGDKVMANGVYYWYA
                     EFKDWYGTVHKKQGEIVLVR"
BASE COUNT        13525 a         5982 c         6591 g        13540 t
ORIGIN      
        1 agtctaaaaa agttgtttac cgaacctaaa acttcatatt ctacataaaa agcattagtc
       61 cattcagtaa aagctttaaa ctcgtgcatg ggaacgctaa actcgtcgaa aaacaaaata
      121 tctccagatt ttaaataagg atacaaagtt gttaaaataa acaaagtaga agagtataaa
      181 tcagcgtcta ggtgaataac tttgcggttt tctggattat aatttttcaa aaaaggcaat
      241 aaagattgtt gaaataatcc ttgataaaaa cttgctcttg tgtcatcaat ttttggtata
      301 gcattgtctt ctattgcaaa atgtccagct ttaaaagttc cccaatcttc gggtaaacca
      361 gtaaaagtgt cgaaaccata aaatctagaa tctttatgag taatatgttg tacccaccat
      421 ttaaatgaca cgcctttata gacaccaaac tcaaaaaaat ctattggaat ttcatttagt
      481 ttttgagttt caatcacatg caaatacagt ttcttttttt tattgaaatc atattttggg
      541 gaataataat cgctataccc atcgttttta tgtttattta caaaacgaga aagctttgct
      601 agatgaccga taaaaagaaa aatgtttgaa ggcactaagc ggtgaagacg taaacgataa
      661 aatatttctt tgtgtttagc tttcatacaa cttttgtttt atgaggcaaa agtaaataaa
      721 aatatgtaat acttaaaatc tgtaattata aaagtaaaaa tattttcact aagcgattta
      781 tttttgcatt tttgtaaaaa atattaaaaa tgcataaatc tggttttgta aatataattg
      841 gcaacccaaa tgttggaaaa tcaacaatta tgaatgagtt ggtaggcgaa aaaatttcta
      901 ttattacaga aaaatctcaa actactcgac atagaatttt aggtattgta agtggagata
      961 attttcaaat tgtttatagc gacactcctg gaataattaa gccaaaatat aaactacaag
     1021 aaaaaatgat ggactttgtt actacttctt ttgaagatgc tgacatattc ctttatgtaa
     1081 ctgatgttat tgaaaatcct aaaaaaaata ttgagtatat cgaaaaactt agtaaggtta
     1141 atgtgcctgt agtttgttta ataaataaaa ttgatttagc cgaccaacaa aaagtggaag
     1201 aacttcaaga attttggaaa gaaaacttgc caaatgcgga gatttatgca atttcagctt
     1261 tagaaaaatt taatattgaa ggtttattcg atagaattat tgaacttttg cccgaaggac
     1321 cagcttggtt tccaaaagac cagctaacag acaaaacaga aaggtttatt gtttctgaaa
     1381 ttgttagaga aaaaattttt aaaaactatc aaaaagaaat tccttattct tgcgaagttg
     1441 ttgtagaaga atttaaagaa gaagaaacta ttattagaat aaaagttaat attattgttg
     1501 agagacagtc gcaaaaagga ataattatag gacataaagg agcttcaatt aaaaaagtgg
     1561 gaactcaagc aagaatagat attgaaagtt tttttgagaa aaaagttttt attgaatttt
     1621 ttgtgaaagt caaaaaagat tggcgtgata atgataaaag cttaagggat tttggatatg
     1681 tgtaagagtt aaaagtttgt aaagttcata aagtttgaac cgagcttgcg agctcgctga
     1741 aagcaaaagt tcataaagtt cgtaaagaag aaaagccgaa cttgtaagct cgattcaact
     1801 tttaataatt tttatctaat tagagtaaaa taaccagttt ctgaataatc atttccgtat
     1861 aaatctttgt attgtacata ccaagtatat acgccaacag gacattccat atcattgtat
     1921 cttccgtccc attcttgttc gtagtcttcg gtgttaaaaa ctatttcacc ccatctgtta
     1981 taaacaataa aacgccattc ttttgtattt gcgtttgcaa caaatactct aaacttgtcg
     2041 ttcataccat cgtagtttgg agtaaaagct gtaggagcgt aaatggtatg ttctgtattt
     2101 acaacaatta tcttttgtgc tgtgtctgaa caagagtata gagattctgc ttcgagagta
     2161 acagtatagg tgcctggagc agaatacgtg tggctaggat ttctatcttt gctagtgttt
     2221 ccatcgccaa aatcccagtg ataccaaaaa gcacctacag aataattaga aaaattaact
     2281 aatggaaatt cagtgctaac agattctcta tctgctctaa aatcaacttc tggtactgga
     2341 aacacagtta ttccaattgt tgagtcaaca gcacaattgt tttttgagaa aatagttaaa
     2401 ttaacactat aagttctagc attttcaaaa atatgtgttg ggttttttgc aaaagataag
     2461 ttttcaaaat cgccatcatc aaaattccaa acatatcttt gaccttggtc gggactagtt
     2521 tcctcgaaat taacttctaa aggagaacaa ccactgtatc tgtctgctct tgggttaatt
     2581 aatggaagtt cgtaagtaga aatgtgtata tcttttgtta cttctgcaaa accacaaaca
     2641 tcttgtactc taaatctaaa aacagtgtcg ttataaattg ttagacgaat aggattagac
     2701 attaaagaat cgtttaccca aacattgttg ttagttaatc ctcctccgct aactgttgca
     2761 gtacaggtaa cttgagatcc ggggcaaact atattgttag aaacacttac atttaattca
     2821 agtggtttgt gtacataaac agtaatttct atatcttgac taacacaaaa accagcatca
     2881 acaacatgag cggaataagt tgttgtctct gttggactta ctagtgtggt cattccttgg
     2941 tcatcattgt cccaaactat tgtgtatggt gctacgccac cagtaattat tccaatacct
     3001 atttctgtag tcattccata acaaataact tggtcgtcca tagcttgtag gtaaaaaggt
     3061 tcaggttctg ttatttcgta ttccataaca tgatggcaac cactttggtc tgaaatagtt
     3121 acgctataaa taccaacacc aacacctatt aaattttgtt cccaacttcc gtttgaccat
     3181 tcgaaatgat atggaggacg accaccttgt gcagttatgt aaacatttcc atcattagtg
     3241 tttccttggc aacttacatg agttccactt attgttccgc ttaaaggagc tgaaggttgt
     3301 attatttgaa cagtgtcggc atgtgtacaa ttattatgat ctgttatggt aaccatatat
     3361 gtaccaggaa ttaaattgtc aatatcttca ctaatagcac cagtattcca tgagtaataa
     3421 tatggttcag ttccaccaac aacagttagg tctgcaactc catttccttc gccaaaacat
     3481 cttacatgag taggagtaat attgccatgc aaaggtagtt gaggttgttc taaaattgca
     3541 tatccaaacg aagagcaacc attagcatcg ccaacggtta caatataagt tccgaaaatt
     3601 aagttttcta agttttggct agtttgtcca gaattccaat taaaagaata tggagttgtt
     3661 ccacctacaa catttaaaag aatagagcca ttattatttc cgtaacagtc aatattagtc
     3721 ggataaatgt gtaattctaa ttcatcgggt tgagttattt caaattctgc ggataaagaa
     3781 cagttattat aatctgtaac agttacaata tactcaccag caacgatatt gtttagagat
     3841 gaagtcatta gtccatttga ccaattatat gttaaagacc ctgttccacc agaagcatta
     3901 aattcaattc ttccagtctg gtttccataa caatctacat cttgagtgtt tgcaacatag
     3961 cttaatggtg ttacatcgtt tattgtaaat tctgcttcgc ctgtacaacc attgttatct
     4021 gttacagtaa attgatagtc tccagcaaca agtgttgttt gttcaaatcc tgtaatatta
     4081 tctggattcc atgcaattgt atatggttct gttccaccaa caacattaac tctagcagtt
     4141 ccattgctgt cgccataaca taaaatgcct tgaatattag taacattaac aaccaaactg
     4201 ggttgattag ctatagtgcc tgttgccgtt gcagaacaac cactattttc tgttacagta
     4261 accgaatatt ctccaggttc taagtttgtt aagctttgtg ttgtttgttg aggatttgta
     4321 ttccaaatat attgatatgg ttgatgtcca ccaaagacgt ttgcagaaat ttcaccatca
     4381 ttcatattat tacaagtaac atcttgtgtt aaaattccta ctgataaatt tataaattca
     4441 acgaaatatg tagaacttgc ggaacagcct actgcgtctg ttgctgtaac agaataagtt
     4501 ccggtatttg caaaagtata tgaattactg atagaattat cttgccattg gtaagtaatt
     4561 cccggatcat ttaaagtaat tgtaaaagga tctccattac atactattgt gtcgtttaac
     4621 tctgttattg gcggaaggtt tgaattaata ttaacttcca ttgaaatatg ctcttgcaca
     4681 caagcacctt cgcgtaagaa gtacattgta actgtataag tgtctgttac tgccggatat
     4741 tcgtggttga taacttgatt gccaacaact ataggagaac cgtctcccaa atcccattct
     4801 acattagtaa tatcaggact tgttgcttgg aaacttacag ttgtttgagt acacattaaa
     4861 gtatcttgaa aaacatcaaa agctgtagat gtgccgtcaa caacaatatc aaaactaaca
     4921 tttaaaggaa ctatattagc tccagctaaa tagccataag attcaacatc tccaaatcca
     4981 taaacagtag ctaaaactcc ttcgggagat attatatagt gagagccttg agttaagttt
     5041 tttctaacat aagaatatgc gttatttgca ggaactgtag cccagccagt tagtggagag
     5101 ccatctaaaa gaacctcaga agtggtagat gttttagcta caatatttgt ataaaattgg
     5161 ttgattgctg tagtattaaa tgcttgaaaa actatataat ctaaagtttg ttctattgga
     5221 gaaagcataa tcataaaagg gtcggaagta acgccatcac aacttgttcc ttggctatat
     5281 tgagcaacag aaataggttt gtcgctagta atgaatgaag tggtagataa atttacttca
     5341 tgaaattgac cagcattcaa attaatgttt gctccaccat ttatatttac tatagtagcg
     5401 tcttcgcaag ctaaaattct atatgtatca ctagctctgg tcattaaagg aacagtaata
     5461 tagttatttc cccatgcttt tactggaatc atttgttcgt ataaatggtc gcaataagta
     5521 caacttaaag gaacgttagc acaagtatta cctgcaaata ccgcaaaatt atggcagttg
     5581 cccaattctg tagattttac agttgaacca gtgaggtcgc ccaaactttg aactaaatac
     5641 acttcaccag catttaaagt tatggaaaat ggaatatttg catttccgcc acctccactt
     5701 aaatttgctt taggagtaat ttctattgaa gttccatctt ctataccaac aacaataaat
     5761 tcagatggtt gtgagctaaa atgagaatag gtagttacca aataattgtc gcctaaagca
     5821 atagttggaa atacaatagt agcatctgat gaggcggttc tttggtttgc agcataaaca
     5881 gctacaggag tatcagattg tactctaaca gctctattta aaactgtatt aggttgagat
     5941 atacaagcat tttgtgcagc aggaacttct atatattgag aaccatttgc accaatagta
     6001 aaatttacag accaacctga gccaggaata ctaacagttc ctgtagctcc aacatcagaa
     6061 gtaatgtata tttgtgtact tacaggagtg ttgaaatttt gcataaaaga taaccaaaag
     6121 tcagttccgg tagtactttg agcaaatagt tttgataatc caaaaatagt taaaccaaaa
     6181 atggttataa gtcttttcat tgatcaaatt taataattat taaaagatgc taaattacag
     6241 tgaaatttaa gtttttacaa ataaaatcca ttgtttttag ctttattcca taaaatataa
     6301 cgttattttt taattttggt ttattttttt ataaaaaatg ttgtgaagtt ttgtaatttt
     6361 caaaaaattt tataacttaa acgataataa gaaaactatt aaagtttgaa gaatgaaaat
     6421 attagttatt tttgcaaata aatattgaaa atttaaaaca taataatatg gaaaaattaa
     6481 aaagtggtga atttcttgta aaatcaacaa attcttcgga tatatttatt ccagaagaat
     6541 atactgagga acaattaatg attgctcaca tgattcaaga ttttcttgat gcagaagtaa
     6601 atcctatagt tgcaaaacta gatgaaggca atcgtgaact tatggcagca accattagaa
     6661 aagctggtga gcttggttta atgggtattg ctattcctga agaatatggt ggatttgaac
     6721 aagattttgt tactcaaatg cttgcagccg aaaatattgg tgcaggatat tctttctctg
     6781 tcgcttttat ggcttcaaca ggaattggga ctttaccaat aatgtattat ggtaacgaag
     6841 aacaacgtca aaaatatgta acaaaactag caagtggcga atttattggt gcttactgtt
     6901 taaccgaacc tgacgctggt tctgatgcaa attcaggaaa aactcaagca gttctttctg
     6961 aagatggcaa acattatatt cttaatggac aaaaaatgtg gattacaaac gccggttttg
     7021 ctgatactca cgttgttttt gcaaaaattg ctaacgacag agttctttct gcatttattg
     7081 tagaaaaaac tatgccaggt gttgaaattg gtcctgatga gaaaaaaatg ggaatcaaag
     7141 gttcttcaac ttgccaaatt tattataacg atgtaaaagt tcctgttgaa aatttaattg
     7201 gaagaggcgg aatgggattc agaatcgctt tgaacatttt gcacatggga cgtattaaac
     7261 ttggagctaa tgttattggg gcagcaaaaa aatctataag cgatgcagta aaatatgcaa
     7321 acgaacgcaa acaatttaac gttctaattt caactttccc aacaataaaa cacaaaatag
     7381 ccgagcaagc aataagaact tttgctttag aatctgcaat ttatcgcgca agtcatgata
     7441 ttgattgctt gatgaaaaaa tataaagccg aaggaatgga taaaggacta gctgctattg
     7501 aagctattgc tcactatgct gttgaagctg ctttattaaa agtatatggc tcagaagtat
     7561 tagattttgt tgttgacgaa actgttcaaa ttcatggtgg aatgggttat tctgccgaaa
     7621 tggatgcaga tagaggttat cgcgactcaa gaattaatag aatttttgaa ggaacaaatg
     7681 aaattaaccg tttgttatta gttgaaacag ctatcaaacg cggaatgaaa ggcgatttcg
     7741 atatggcagg accagctaaa gagttttatg aaaatatgga aaattgccgt atcaataaaa
     7801 aagatggaga aaatttcttt gaacaaaaat taagatatat tgaaaatttc aaaagagctg
     7861 cactaattct tattcacaaa gtttcagata agtatgaaaa agatatgctc agagaacaag
     7921 aaattatcaa taatatcagc gatatgatga ttcaaattta taccgcagaa tctttaacac
     7981 ttagagttaa gaaaattgca gaaacaaatg caaaaccaaa tcttgaatta tataaagata
     8041 tagtaaatgt tttggtttac gaagctgctg cagtgatttc taaaaatgca aaagatgcag
     8101 ttttctcaat gacagaaggc gaagaaagcg aaaaaatgta taaagcactt aaagacttat
     8161 cttgcgttga aggagtaaat acaaaagatg caagaagaag aatcgctgat ttcttaattg
     8221 ataaaggcga atataaactt taaaatttgt ttcatgtgta ataaaaaagg ctactttatt
     8281 tttaaggtag cctttttctt ttttgaaatt tccttatcta tatatctata agaatattag
     8341 ttgtaccaaa atatatacag gcaataaaac tattaaagag aatttaatca tataaccaaa
     8401 gaaacttggc attttaattt tactttcttc ggcaattgct tttaccataa agtttggacc
     8461 gtttccaata taagtcatag ctccaaaaag tacagcaccc aaacaaattg cagttaaaat
     8521 catttcatca acacctgcca cataacgaac accttcaact acgctaccat cattcattcc
     8581 gagagctaag ttataaaaag ctaatgcagt aggagcattg tctaagaaag cgcttaaact
     8641 tcctgttgca taataaaatt gagcatttga gcttattcct aaactattag aattttctga
     8701 taaatatcgt aaagcaggta tcatagtaag gaaaattcct aagaataaga atgctacttc
     8761 taaaattggt ccccaagaaa atttgtttgc ttcgcgtata ggtttttttg taaataataa
     8821 agacaaaaca gccatagaaa caatacttcc ttctctaata aatcctgccc aatcactttc
     8881 tgccataata ggtaaatatt gtttgtttag aaatgcaaca gaagcaacaa cacctgctag
     8941 gaaaataaag ttaatatttc cacttaaacc caaaggttca acatttgttt tgtcgaattt
     9001 tatattttca agtggttctt ttttataata atatgtatca gcaataaagt aaattaataa
     9061 tagcaaggca tttactaaag cccattcagg aagcatatgg aaaaaccaag taaattcagc
     9121 accttttaga tataacatga aaagtggtgg gtcgccaagt ggagttaata atccaccagc
     9181 atttgcaaca atggcaatga aaaataaaat tgtgtgaact ttaaattttc ttgcattatt
     9241 tgttctaatt acaggacgaa ttaaaagcat tgctgcacca gttgttccca ttatagaagc
     9301 taaaatggca ccaattccta aaaaagaagt gtttatccat ggttttgctt gaatattgcc
     9361 ttttaagtga ataccaccag ttattacaaa taaagctcct aataaaatga taaaaggaag
     9421 ataatcaaaa atcaattggt gtgctaaaag ttctggtaaa cctaatgcta tcaaatatcc
     9481 tgcaacagga ataccaagta ttatagacac aagtaatttg tttctgtttt tttcccacca
     9541 gtgttcaaaa aacaaaggac ctaatgcaat acataataac attaatacaa aggggataat
     9601 agcccaaaga ggcatgttta cgttagttgc tgataataaa gtcataatta aaatttaaaa
     9661 ttcattaaat gcaaaattaa ttatattttt ttaaagtcgt tattttacaa gaaaagatta
     9721 aggtttatat ctctatgttt gacaacagaa ttttcgtact gttgagctgg taattaaacc
     9781 attactttat taaatttaaa acctctttag ccaatttata ggcttgagat tttaaataag
     9841 gttttctgtt tttagagttg atgcaccaat gtcgaacaac tacgccttgc ccgcaagggc
     9901 aacatgtgat taaccgtaag atttatcttg cggtagagat gcctctcagt ccattcgccc
     9961 gcaagggcga cacaattaag ttgttaaaag tgatacgcta aacgtagttg aagtgttaaa
    10021 gttgttgtgt tgccctacgg gcaaggcttt cgagggtcta tataccgcag gctgcgccta
    10081 cggttaatca catgttgtcc tacggacaaa taaaaatatg tgcgttgttt tcaatgttat
    10141 aatacccaaa caaacaaatt gcctgattgt caagaggtta gctggtgttg caaaaaatgc
    10201 ggaaaacagg aaattatgtt atgagatgtt aaataagctt ttagtctctt atacttatca
    10261 aatttttaca agaaaataag ttgtaccaaa atatatacag gcaataaaac tattaaagag
    10321 aatttaatca tataaccaaa gaaacttggc attttaattt tactttcttc ggcaattgct
    10381 tttaccataa agtttggacc gtttccaata taagtcatag ctccaaaaag tacagcaccc
    10441 aaacaaattg cagttaaaat catttcatca acacctgcca cataacgaac accttcaact
    10501 acgctaccat cattcattcc gagagctaag ttataaaaag ctaatgcagt aggagcattg
    10561 tctaagaaag cgcttaaact tcctgttgca taataaaatt gagcatttga gcttattcct
    10621 aaactattag aattttctga taaatatcgt aaagcaggta tcatagtaag gaaaattcct
    10681 aagaataaga atgctacttc taaaattggt ccccaagaaa atttgtttgc ttcgcgtata
    10741 ggtttttttg taaataataa agacaaaaca gccatagaaa caatacttcc ttctctaata
    10801 aatcctgccc aatcactttc tgccataata ggtaaatatt gtttgtttag aaatgcaaca
    10861 gaagcaacaa cacctgctag gaaaataaag ttaatatttc cacttaaacc caaaggttca
    10921 acatttgttt tgtcgaattt tatattttca agtggttctt ttttataata atatgtatca
    10981 gcaataaagt aaattaataa tagcaaggca tttactaaag cccattctgg caccatgtgg
    11041 aaaaaccatg aaaattcagc accttttaaa tataataaga aaagtggtgg gtcgccaagt
    11101 ggagttagta gtcctccagc atttgcaaca atggcaatga aaaataaaat tgtatgaact
    11161 ttaaattttc tctcgctatt tgttctaatt acaggacgaa ttaaaagcat tgcagcacca
    11221 gttgttccca taaaagaagc taaaatagca cctattccca aaaaagaagt gtttatccat
    11281 ggttttgctt gaatatcgcc ttttaagtga ataccgccag ttattacaaa taaagctcct
    11341 aataaaatta taaaaggaac ataatcaaaa agcatttgat gttttaaatc acctgtcaac
    11401 ccttgtgcta ttaaatatcc tgcaacagga ataccaagta ttaaagatac aagtaattta
    11461 tttttgtttt tttcccacca atgttcaaag aacaaaggac ctagtgcaat acataatagc
    11521 attaatacaa aggggataat agcccaaaga ggcatagact tagttgctga taataaaatc
    11581 ataattcatt aatttaaaat tccaaaaccg caaaaataat tatatttttt aaatgcaata
    11641 attttataga taaaagatta acaaaacctc tatgtttgac agtagattta ttgctctgat
    11701 gtttctgaat tagaactttt cttttttaat ttcaagcttt cgagtatgct ttctccaaaa
    11761 attcttttat aaaaaattag taataaaaat acagaaactg ttatagcaga gtctgccaca
    11821 ttgaacacag gtctaaaaaa caaaaattct ttaccaccaa caaaagggaa ccatgttgga
    11881 tatgtggttt ctattattgg gcaataaaac atatccacaa ctttaccatg gagaaatgga
    11941 gcatagccac cttcaggagg aaacattgtt gctacttggt aataggtact ttcattaaaa
    12001 attagaccat aaaatgcaga atcgattaaa tttcctgttg ctccagcaat aattaaggaa
    12061 atgcaaatta tataaaataa attttcgcca gcttttataa ttttacataa ataatatatt
    12121 agtaatgaaa ttgctacaac tctaaataaa cttaaaaata attttcccca actaccacca
    12181 aatgtcattc caaaagccat accttcgttt tcaacaaaat gcagttttat tttgtcgcca
    12241 atgatattaa gttcttgacc tagagtaaag ttagttttta tccaaatttt taaaacttgg
    12301 tctaaaatta aaattcctaa aattatagct atagctataa gtgatttttt tctcataaaa
    12361 atcgttattt tgaactgcaa atataaatta ataattcatt tatcttattt agaatgtata
    12421 taaatgtccg ttttaaaatc aaaaactttt aaaatcattt ttatttaatt aaaaagaaat
    12481 taattttgtc gtgttaaatt ttgaattatt aatttttaaa gaatagaaaa aatggcttac
    12541 aaaattactg acgactgtgc cgcttgtggt acttgcatag atgaatgccc agtagaggca
    12601 atcactgaag gtgctattta tgttattaat ccagaccttt gcatcgactg tggtgcatgt
    12661 gctgatgcat gcccagtaga ggcaataaag cctgcatagt taagaaatta aaaagctcct
    12721 ttcggagctt tttttttgga gaaaagtatt cttaaaacta atttcaagca agcagagtga
    12781 gctagctcac tctgcttgct cagatttttc cacacatccc cgctcgctcg gatttgtaat
    12841 ccgagtgtaa cattactaag cactcggatt acaaatccga gcgagcgtca taggtgtttg
    12901 agcgagcaat agtggtgata ggagcgagct ggtgtgggtt gtccgtgagg ataaaacaag
    12961 accatcaaaa gtttgtccgt aggacaattc agtcaaaagt ttataaagta aaaagttcat
    13021 aaaggaatgc caagcaaaaa acttctttta acttttaact ttcaactgag ttttacatag
    13081 ctgctaagag caaattaatg tctttagcca taagtccatg aatttgacca atattttcgt
    13141 ctgttaatat gccatcataa atatacactc catatctaac tccagcattt gtttttagat
    13201 aggaattaat acttcccgct gtttgaattt tgagcaactc aaaagataaa atattgctta
    13261 ttgaataaga agctgttctg gcaactcggg aggcaatatt tggcacgcaa tagtgaataa
    13321 caccatgttt agtaaaaaca ggagatttat gagttgttgg tttacttgtt tcaaaaattc
    13381 cgccttggtc aatgctaata tcaacaatta tagattgttt tttcatttgc ataaccatgt
    13441 cttcggtaac aataatatta ggtctattat tattaattct taccgcacca ataacaacat
    13501 cagcggtttt aattgcagct aataaaactt tagaataaat tgtgcttgta aaaacttttg
    13561 tacctattaa actttgtaat ttgtcaagtt tagcaatgga gttgtcaaaa acttttacat
    13621 gagcacctaa agcaatagct gtttgtgttg caaaagtgcc agcaattcca gctccaataa
    13681 caacaatctc gctaggagaa acgcctgtaa ttccacctag catttcgcct tttcctttat
    13741 gtatattatt caaatattcc gaagcaatta gcaccgccga acgaccagca atttcgctca
    13801 ttgatcttac tattggatta gctcctttgc tatctcgcat catgtcaaaa gctaaagctg
    13861 taactttttt cttcatcaat atttgaaaat attcttttgt ttgatatgtg gagtgcaaag
    13921 atgttataat aatttgattt ccacgaagta agaccatctc ttctattgtt aaaggacaaa
    13981 ttttaacaat aatatcacaa ctaaaaacat cagcaagatt tgttgtaatt tcagctccat
    14041 attttgaata ttcgtgatta gaaaaataag ccccttttcc cgctccatct tgaattacaa
    14101 cttcaaatcc ttcgtttaca agcacttcta ctgcgagggg tgctaaagga actcttcgct
    14161 cattaccagt tttgtcagct ggcattccta ttctaaactt tttcttgtcc tgaactaact
    14221 ccaacatttc ttcttgtggt aaatatacag aaccaggaat aaaaacattg gttttttgct
    14281 tttcggtcat aatttttatt ttttataaaa atactgattt ttttttaatt aagctcaaac
    14341 ttcctttaca atttcagaat aatctaattc aaaatctatt tgtttcttct gtaagtttac
    14401 atttacaagt tttactttag tttcgttacc aagttgaaaa acttttcctg tgtaacttcc
    14461 aactatacaa tattcttctg cattaaactc ataaaaatca tctgttaatg ttcgtatatg
    14521 caccaatcct tcgcaagcat tttcttttaa ttcaacaaaa aatccccatt ctgttacgcc
    14581 agaaataatt ccatcgaaaa cttgcccaac tttatcttgc ataaactcaa cttgtttgta
    14641 ttttatcgaa gctcgttcgg ctaaacttgc ttgttgttct tggtaagaac aatgtttaca
    14701 attttgttca agttcttcag tatcgggttt atttttgcgt aaatagtcaa aaatcaacct
    14761 atgaaccata atgtcggggt atcgccttat tggagaagta aaatgtgtgt aataatcaaa
    14821 agctaagccg taatgaccaa tgtttttagt tgtgtaaaca gcctttgcca ttgctcgtat
    14881 agccaaattt tcaataatat tttgttcagc ttttccatta acatctttaa caatattgtt
    14941 aatagatttt gccaaagaaa ttttattttt ttggtcaata gtatatccaa aacgtgaaat
    15001 aaaatttgaa aaattcataa gtttttccat gttgggttca gcatgaactc tatataccat
    15061 tggttttgga gattttattt ttccataaat ttcggcagct tttctattag caaggagcat
    15121 aaattcttca attaaatgat ttgaatctgt catctctttg aaataaacac ctgttggtac
    15181 agcatttttg ccaagaataa attttacttc tgaatgttca aagttgaaag ctcctttttt
    15241 aaatctttct ttgcgtaata ttttagctaa attgttaaaa gtttgaattt cttttgcaaa
    15301 atcgccttcg ccagtatcaa taattttttg tgcttcttcg taagtaaatc ttctatcaga
    15361 attgataatt gtttttacaa ttctatagtc taagacttca gctttgtcgt tcattttaaa
    15421 aattacagaa aagcatagtt tatcttcgtt tgggcgtaaa gaacacaaat aatttgaaat
    15481 tctttctggc aacattggca caactctatc aaccaaataa accgaagttg ctctactaaa
    15541 ggcttcgttg tctaattcag aattttcttt aatatagttt gtaacatcag cgatatgaat
    15601 acctatatta tataagtttt cgtctaaaaa ttctattgaa agagcatcgt caaaatcttt
    15661 tgcatcttcg gggtctatgg taaatgttgt gtgagaacga aaatcttcgc gtgtttcaag
    15721 gtcgtattga gtaattttat ctgatatttt ttcggcagct ttgtttattg cgtctggaaa
    15781 tttatgtggc aaaccaaatt ctgcaagtat tgcatgcatt tctacttcat tctctccagg
    15841 gcgaccaaga atttcaacaa cttttcctgt tggatttttg ccttttctgt gccattccac
    15901 aacatcaaca acaactttgt cgccttcttt tgcattgttt aaatagttgt taggaataaa
    15961 aatatctaaa ggcatttttt ggttgtcagg aattaaaaaa gcataagtgc tagatttttc
    16021 aataactcca acaaatcttg tgtttgctct ttccaaaatt tctacaactt cgccttccat
    16081 tttgccacct cttctaagag caaataaaca aactttaact ttatcgccat taagagcaga
    16141 gcgtaatttt gtaaaaggaa taaaaacatc ttcagaaagt tcgtcgctaa ctaaaaatgc
    16201 ttttccactc tttttcaaat caacagttcc aatgatatga ctgcttttag atttgagttt
    16261 gtatttccct ttagaaacaa tttttaaatt acctatgcca actaattcgt caagagcttt
    16321 tataactaaa tctctattgt gagaatcatt tattgacagc attgaagaaa tttgtttgta
    16381 attcaactct ttttgtggag atttagtaaa gatatttaaa atttgtgaag ttaaggtttt
    16441 cttgtttaga gaattggttt cgggttgttt ggagtttttt ttctgagttt ttttcatttt
    16501 tggtattgtt tttgcaaata taaaaaaaga aatttaatat ttatttttgc gttattaaaa
    16561 aatatttatt tatctttaac aaaaatttaa aagtagtaaa aattaaaaat tattagtatg
    16621 agaacagtta ctttaatgaa aagcatttta gtcatcgcaa taatcagcac agcgatggtt
    16681 ttttcttctt gcaaaaaaaa tacaaaacca ggaggaaaga aagcggttga aattgaaatt
    16741 ccatgtaaag atgaaggtcg ttctgacaag aattttttca gagcagatgc aaacgcaact
    16801 tcacaagaca tgagtttatc tcgcgaaaaa gctcttttag ctgcaaaaca aagagtttca
    16861 agtttgatta attcaaaaat taaatctgta acagatcgtt atgttaacga aacagaagtt
    16921 aatcaaaatt cagatttcga agcaaaattt gaaaatttaa ctcgtgaagt tgttaatcaa
    16981 acattagtag atgttgctat tacttgtgag aaaaatttcc aagaagcaac tggaaaatat
    17041 actacttata ttgcagttga agttagcaaa gatgctatga taaatggaat tgaaaaaggt
    17101 ctttctaaag atcaaaagct aagagttgat tatgacaaaa tgaaatttga acaaattttc
    17161 aacgaagaaa tggacaaact ttctaaagaa ggtggctatt aaaaaatgaa atttttgaaa
    17221 atgattttta aagaagaggc ttttgcctct ttttttttga aataatttta caacattcgc
    17281 tcgctcggat ttagcttcgc tgagttcgtc ctgcggactt cggttgtaat ccgagagttt
    17341 ggaaatgtta cactcggatt ataagttcga gcgagcaggc ttatttgccc gtagggcaac
    17401 atgtgattaa gagatgtctc aaaaataaga gttaaggcaa caagctttca aggtgtttca
    17461 gaagtgttaa aatgcaaggc gtctgagatt gagaacgaag gagcgtacca gaagcggtgc
    17521 actgagcaaa gccgaagtgt acgtgactga gttcgaacat cgaaagcaac gtggttgctg
    17581 aacgaagttg aagcaaagca gtttgacact tatgaaacac cgtctatttt tttaaaaaag
    17641 ttttagttgc ttatctaaaa gcttatagct ttttctatga tattttgtta ttccaaattc
    17701 taaaattgct tgtctgtgtt tttttgtagg atagcccata tttttattcc agtcgtaatt
    17761 aggaaattcc tcatgtacat taagcatgaa ttggtctctg tgtgtttttg caagtatcga
    17821 agcagcagca atcgacaaat aagtagcatc accttttaca atacatttat gtttaatttc
    17881 tggatatggt ctaaatctat ttccatcaat aagcaggaac tcaggttttt gttttagttt
    17941 ttctattgct ctgtgcattg acaaataact tgcattgaga atatttattt tatctatttc
    18001 ttcgtttgta caaaaaccaa tagcccaagc aattgcattt ttttcaattt caattcttag
    18061 ttgttcacga gtttttgaac ttaattgttt ggagtcgttt aagatttcgt ttttgtagtc
    18121 aggaggtaaa attactgctg ccgcataaac aggtcctgca agacaaccac gaccagcttc
    18181 gtcgcagcca gcttcaattt gtccttctaa gtaatattgt ttaagcatga ttacttgctt
    18241 gtattgcttt ttcgacacat tcccaaagtc tttcaccaga tttttcccaa gaaaattttt
    18301 gcctttgaat tttccctttt tctattaaat cttgtctaag ctctgagttt aaagcaatgt
    18361 tttccatgtg ttgagcaact tctttatgat tgtttggatc gcaaattata gcagcattac
    18421 caacaacctc tggtaaagaa gttgtattcc ctgaaataat tggaatttcg gaatacattg
    18481 cttcaactaa aggaattcca aagccttcaa aaaatggaat ataaactaaa gcggtagatg
    18541 aggcataaat taaatgcaat tcatcaatat ttaatcgtcc agtaaaaaca acatctttag
    18601 aaaatttcat ttctttatat gttttatcaa tttcgcctgt gagatgcatg gcagagccga
    18661 caataactaa tttatgtggt agtttggaat tttctttaaa gaaatcaaaa gattttaaaa
    18721 gccctggtat atttttacgt ggagttaaag ctccaacaaa atggaaaaat tcttgcttgt
    18781 cagtatattt ttcttttact ttaatttttt cgctttctgt tattggctta tatatgtcgt
    18841 ttgaagtatt gtaacaaaca gtaatttttt cgggagcaat tttaaaaaca tttatcaaat
    18901 ctttttttga atattcagaa actgttccta tatgtttagc ttttttagca aattttggaa
    18961 aaaatttatt ataaaatttt cttacccaaa aaggtaattg ctgaggattg tgataaaaat
    19021 taatgtcgtg aataacaaca gcttgtggaa tttttgtatt tagtgaaaca aaaccatctg
    19081 ttgaaagaaa aatatctgca ttaacttttt ttaatactct aggtattaca aattttaacc
    19141 aaaaccacca taaaaatgga tgtcgagtag ctggaccagc aacaattggt ttaacattat
    19201 ctgcaaaaat aaaagaaggg tcatagtttc tatcaaaaat aaaatagaaa ttatgttcgg
    19261 gatgtgtttt tacaatatgt tttaaggttt catatgaaaa ccaacctatt ccttcgagct
    19321 tattttttag tagtagtcta gtatttactg caatattcat tataaaagtc caagttgttt
    19381 aactttttct aaatctttag gcgtgtctat agaaatactt tcgtgttcgg taaatgcggt
    19441 tttgatttta taaccgtttt ctatccatct caactgctct aacgactccg ctttttctaa
    19501 tttagaaact tcaagtttag ttatttcaag caaaatatct gttctatatc cgtataagcc
    19561 aatatgttta taaaaagaat gtttagtaac ccatcttgtt tcatgaactc ctcgcaaaaa
    19621 tggtattggc aaacgactga agtaaatagc ttcattttta ttgtttataa caacttttgg
    19681 cttatttgtg ttaaaaatat cttctttatt atttataggt tttattaaag tagcgatttg
    19741 tgtcctttta gatttaaaac atttttttat ttctttgagt tgttctgttt ttataaaagg
    19801 ctcatctcct tgaacattta ttacaacatc ccatttttta ttttttaaag tttgaatttt
    19861 ttctattgct tctgcacatc tgtcggttcc gcttttatgt ttcttttttg ttaatacaac
    19921 atttccgcca aaagattcta cttcattaac aattctaaca tcgtctgtag caacccaaac
    19981 ttcgtcaaaa actagtgatg cttgcacata aacattatgt accattgatt tgttattgat
    20041 ttgaaccaga ggttttccag ggaatcttgt tgatccaaat ctagcaggaa taattgcaat
    20101 aaattccata aaataaaatt ttgcataaaa ttactaaaat ttatttagtt tgtaatatat
    20161 ttttgcaaat tttgcaattt gaaattatgg aaaataaaaa tacagatact tctaggtttt
    20221 ttaaatggtg gaaaatagct attccaatag ttctcggttt gggagtagtg ttgtattttg
    20281 ttttaagaga ttttaaccaa attgactttt ctttattcaa atttacgaaa tacaccttac
    20341 tatttttgat attggctttt gtaatgttgg tttttcgtga ttttggttat gttttgagaa
    20401 ttagagtttt gtctggtggc gaattgtcgt ggatgcaaag tctgaaaata gttttattgt
    20461 gggaatttgg ctcagcgata acaccttctg cagtaggagg aacagcaata gcaacaattt
    20521 ttttatggaa agaaggaatg agtgttggaa aaagctcggc aattgttttg gcaacatctt
    20581 ttcttgatga attgtatttt agcctgatgt tccctttaat tttgttaatt gtaggaaaat
    20641 cacaaatttt tgtaggtgaa caaaatgtat ttttagaaaa actattttgg tttgcaatag
    20701 ctggatattg tttaaaacta gcttggacaa tattaatagg atattcttta tttgtaagtc
    20761 ctaaattcat atcaaatttt ttgaaaaaaa tatttagaat aagattttta aaaaaatgga
    20821 gagcatctgc cgaaaaagta gcagacgact acgttttagc aaataaagaa tttaaaaata
    20881 aaagttttgt tttttggata aaatcatttt ttgccacatt tatttcatgg acagcacgct
    20941 atttagtttt aaacgcttta tttattgctt tagccgttgg caattatact ttaagttttt
    21001 atgatcatat tttgattttt gctagacagc tgataatgtg gataataatg ttggttatgc
    21061 caagtcctgg cggaagtggt tttgtagaag gagttttctc aagtttaatg tccgattttg
    21121 ttggaattgc cggatttgca ataattatgg ctttggtttg gcgattattt acttactatc
    21181 catatttaat aataggtgcg gttttagctc cacattggtt aagcaagaaa tttaaaagaa
    21241 caaaaaaaga agctgtctcg taagtatcct agttgaataa ttttaagaca gcttttatat
    21301 cttatatctc ttaaaacaac cctacaatat taccttcgtt gtcaatatct atagcttctg
    21361 cagcaggagt ttctggaagt cctggcattc tcatgatttc gccagaaatt ggaacaacaa
    21421 aacctgctcc tgaggcaatt tcaatttctc taatattaac aataaaatct ttaggacggt
    21481 ttcttaggtt tttgtcgtca gaaagtgagt tttgtgtttt tgcaatacat acaggcaatt
    21541 tgctcaaacc taattcatca atagttctaa ggtctttttt tgcttttgct gtaaattcaa
    21601 ctccacttgc tccgtataat tttgttgcta tggtttcaat ctttttttcc atgctccagt
    21661 ctaattcata gagaggttca aaattacttt tgccagcatt aacggtttct acaacaactt
    21721 tagcaaaatc taaagcacca tctccgccat gtgcccacga agaatttaaa gcgtatttta
    21781 cgtttttagc ttttaaatga ttttctagca cttcgagttc tgcttttgaa tcggtaacaa
    21841 attgatttaa agcaactata ggttgaattt taaaatgctg aacattttct atatgtttat
    21901 caagattttc taaacctatt ttaacagctt ctggatttgg atttgctaat tctttaagtc
    21961 ccactccacc atgatatttt aaagctctaa ttgttgcaac aattacaaca gcatcaggtt
    22021 ttaagcctcc atatctagct tttatgtcta agaatttttc tgctccaagt tctgaagcaa
    22081 aaccagcttc tgtaataaca tagtcgctta gagaaagacc catttttgtt gcaataagag
    22141 agtttgttcc ttgagcaata ttagcgaatg gtcctccatg aataattgca ggagtatttt
    22201 ctaaagtttg tactaaattt ggcttaatgg catcttttaa taatgttgcc acagcacctt
    22261 gagcgtttaa gtctcttgca taaataggtt ttttgtcgta agtatatcca acaaaaatat
    22321 ttcctatttt ctcttttaaa tcttcaaggt ctttagataa gcaaagtata gccataattt
    22381 cggaagcagc agtaatatca aatcctgttt ctcttggaac tccttgtgtt tgtccaccta
    22441 atccaataac aatttttctt aaagctcttt cgttcatatc cattactcgt ttccattgga
    22501 ttgttctagg atcaatacca agatttcgag ttttgctttg aatgttatta tcaattaaag
    22561 cggcgagtaa attatttgct ttttcaatag ctcccaagtc gccagtaaaa tgcagattaa
    22621 tatcttccat tggaacaact tgtgcataac cacctcctgt agctccacct ttaactccaa
    22681 aaacaggacc caaagatggt tctcttaaaa caactgttgt tttatgtcct attttattca
    22741 taccttgagc aagtccaata gaagtagtag ttttaccttc gcccgcagga gttggagtta
    22801 tggcagtaac taatattagt ttagattttt ctattttttc ttcatcaatt agatgcaaag
    22861 gtaattttgc tttgtacttg ccgtaatgtt caatatcatc tttactaatt cctagttttg
    22921 cagcaatttc atcaataagt tctagcttag cagcttttgc aatgtcaatg tctttcatag
    22981 tctataagta tttataaaat tataaattta aaattgttct aatataataa taattaatta
    23041 aaatgcaaaa attgttagat attagaaaaa ataaattttt ataatgtctg tgttttaaat
    23101 tttcagaatt gaaaaatata agctattttt acaatcacaa attcaatact taatatagta
    23161 tgaaaaaaat acttgtaact ggtggtgctg gttttatagg ctcacatttg tgcgaaagat
    23221 tattgaacga aggaaacgaa gttgtttgtt tggataatta ctttactggc actaaagcaa
    23281 acgtgataca tttattagac aatccgtatt ttgagcttat tcgtcatgat atcactatgc
    23341 ctttttatat tgaagttgat gagatttata atctagcttg tcctgcttcg cctattcatt
    23401 atcaatataa tccaataaaa actattaaaa cttcagtaat gggttctata aatatgttgg
    23461 gtttggcaaa gcgaataaaa gcgaaaattt tgcaagcttc gacttctgag gtttatggag
    23521 atcctgaaat tcatccacaa cccgaaactt attggggaaa tgttaatcct ataggctcta
    23581 ggtcgtgcta tgatgaggga aaacgttgtg cagaatcatt gtttgtaaat taccataaac
    23641 aaaataatgt aaaaattaaa ctaataagga tttttaacac ttatggtcca agaatgcacc
    23701 caaacgatgg cagagttgtt tctaatttta ttgttcaagc acttacaaac aaaccaataa
    23761 caatttatgg agatgggtct caaactcgtt ctttccaata tgttgatgat ttaattgaag
    23821 ctatgattcg tatgatgaat actaaagacg aagttatagg acctattaat attggtaatc
    23881 ccggtgagtt tacaatatta gaattagccg aaaaaatcat aaaacttact ggttcaaaaa
    23941 gtgaattaga gttttatcca ctgcccgaag acgatccaat acaaagaaaa ccagatatta
    24001 gtttagcaaa aaaacatttg aataattggg aaccaaaaat tgatttagaa caaggattag
    24061 taaaaactat tgaatatttt aaaaaaacta ttaaattatg aaaggaataa ttttagccgg
    24121 aggtgctgga actcgtttgc acccaatgac aattagtata tcaaaacaaa taattccggt
    24181 ttacgacaag ccaatgattt attatcctct ttctactttg atgcttgcag gaattaaaga
    24241 agttttgata atttctacac caactgatat tggttcttat gagcgtttgt ttggagacgg
    24301 ctcacaatta ggaatgaaaa tttcttataa aattcaacct tcgccagacg gactagctca
    24361 agcttttgta cttggagaag aatttattgg tgatgatagt gtttgtttgg tgcttggaga
    24421 taatattttt tatggtcaag ggtttggcag tgttttgtta gaatctgcaa aacttgaaga
    24481 tggagcattg atttttggat attatgtgaa cgatcctcaa agatatggtg ttgcagagtt
    24541 tgacaaagat ggtaaagtaa tttccataga agaaaaacca caaaatccaa aatctcatta
    24601 tgcggttaca ggtttgtatt tttattctaa tgatgtagtt caaaaagcta aaacactaaa
    24661 accatctaag cgtggcgagt atgaaattac agatttgaat aatgtttttc tttctgaaaa
    24721 cagattaaaa attaaacttt taggacgtgg ttttgcttgg ttagatacag gaactccaga
    24781 aagtttgttg caagcttcta attttatgga aactctcgaa gctcgtcaag gattaaaagc
    24841 ttcttgcatt gaagaaattg cttatcgccg tggatttatt gataaaaaac aattggaaaa
    24901 attgattgaa ccacttagca aaagccaata tgggcagtat ttaaaacgga ttttaaacga
    24961 agatatatta tgaaagttgt aaaaaccaat atagaaggac ttttagtgct tgaaccaaaa
    25021 gtttttaccg attcaagagg ttatttttta gaatcttgga ataataaagt ttttttagag
    25081 aataatttgg attttaaacc tgttcaacaa aatgaatcta aatcttctta tggggttgtg
    25141 agaggtttgc attatcaact tgctccatat tctcaagcta aattggttag agtaatttca
    25201 ggaaaagttt tagatgtagc tgttgattta cgcaaaaatt ctccttcttt tgggaaatat
    25261 tattctgtaa ttttgtcaga agaaaataaa aagcaatttt ttattcctaa aggatttgct
    25321 catggttttt cagtactgtc ggattttgca attttttcat atatgtgcga tgaatactat
    25381 agcccagagc atgaaagggc aatttcatat aatgataatt ttttaaatat tgattggcaa
    25441 gttcctttcg ataaaatgat aatttctaac aaggacatgg cagctccttg ttttaaaacc
    25501 gctgaatata atttttaaaa tgaaaattct tgttactggc tcaaaaggac aattgggttc
    25561 aaaaataaaa gacctgtctg ttgattttca gagttttagt ttttcgttta cagatttaga
    25621 cgagttagat attacaaatt ttaattctgt tcttgatttt tttaaatttg aaaaaccaga
    25681 catagtaatt aattgtgcag cttatacagc agtggataaa gcggaagatg attatgaaaa
    25741 tgcttttaaa atcaatgctt taggtccaga aaatttagca aaagcttgta atgttcacaa
    25801 ttctaaattt attcatattt ctacagatta tgttttcgat ggcaaggagc gtgaaaaacc
    25861 atatttagag agtgatttca cttcgccaca atctgtttat ggtagtacaa aattagaagg
    25921 cgaaaattta gttttaaaaa atttacaaga tgccattata gtcagaactg cttggcttta
    25981 tagcgagtat ggtcataatt ttgtgaaaac aatgttgaat ttagcacaaa gcagaacaga
    26041 attatctgtg gttaatgatc aagtcggtag tccaacttat gctggagatt tggcacaagc
    26101 aattttgcaa atttctatgc aatattttga ggaaaattat tggaaatcag gtgtttatca
    26161 tttttcaaat cttggaaaat gtacttggtt tgagtttgct aaagaaattt ttaaaattaa
    26221 aaatattaat attaaagtaa atcctgttac aagcgaagaa ttcccttcga aagtaaaacg
    26281 tccaaaatat agcgttttag ataaagagaa aattattaca acttataaaa tagatgttcc
    26341 tgactggcga gattctttaa aaatagtttt agaaaaactt taattttaaa caagcctgtt
    26401 taaaacggtt tctattaatt tttttacagc aggtttataa tccttactgt attttccagc
    26461 ataacgattt gcaataatca cacaaattgt tgcagcttta tgtcctagca acgcagacaa
    26521 gccaaataga gcagagcttt ccatttcgta atttgtgatt tttttattgt cgtaagagaa
    26581 agaacgaatt ttatcgttta tgtctggatc ttgaatatca agtctaatta ctctagcttg
    26641 tggaccataa aatcctggag cagaaatagt cattcctttt cttatgccgt ctccaagttt
    26701 ttctagcaat tctttagagc cgtcaacaaa atatgggctt gttagtagag gattccaatt
    26761 catgtgtgtt ttaaattctt tttctatatc gagtttgcac acagagttac gctttttgta
    26821 aaaatttaat aagccatcaa agccaattgc ggtttctgtc attaaaaaac tatctacatc
    26881 taggtcttct tgcaagcctc ccgaagttcc tattctgatg aggtttaatg ttttgtgttc
    26941 aggttttggt agtcttgttt ttaaatcaat atttactaaa gcatctaact cgtttaggac
    27001 aatgtcaata ttgtctgttc caattcctgt tgctaaggca gtaaatcttt tgcctttata
    27061 tgttcctgta tgagtaacaa attctctgtt ttgaacttta tgttctattt tatcaaacat
    27121 atccgaaatt gttgctactc ttcctgggtc gccaacaagt aaaactgtgt cggctaattg
    27181 ttctggtttt aaatgtagat gaaaaatact accatcttta ttaataataa gttctgattc
    27241 tgctattggt tgcatagttt aaaaaaatta atttatcttt gtaaaactaa gcaaaaattt
    27301 taatatttca aaaaaaatat aaaattatga gtaaaaaacg aatacttgtt ccaacaaact
    27361 ttactagtga aacagaaatt gcttttgaac aaagcgtttt gttttcttta aagacagatg
    27421 cagatataat tctttttcat gtaataccaa gtttaaagat gataaatagc aatagctcta
    27481 atattgatag agttcaggaa gagttgtcta ttttagcaga aaaatacttt aaagaaactg
    27541 ggaaaaatgc tcaaactaga atagaagttg gaaaagtact tcaacaaatt ttaatgttag
    27601 aaaaagaaat aaaaccttca tatatatttt taggttcaga tgtctcaaaa ggtgaggttt
    27661 cttctttaac tttaaagtta gtaaatagtg tagagtgtcc aattattatt tttcttagaa
    27721 attttaagaa aaagggttgc gaaaaaatag tattaccttt agatttaacc aaacaaacta
    27781 aacaaaaagt ggatcacaca ataaagttag caaaaattta caattctgaa gtacatattg
    27841 tgtcggcaac caactttaca aacgaaacaa aactagcaaa actaaacgat caattagatt
    27901 atgtaaattc tattttcgaa aaattaaata tcccaacaaa aacaaaaata ttaaaaactt
    27961 caaacgacat tgagataatg gctaatgcta tcaatgatta tgccgacgat atagatgcag
    28021 acttaactgt aattatgaca cgtcaagaaa caaaaattca aaagtttttc gtgggtagta
    28081 tggcaactaa attaatcaat aagtctaaaa ttccaatttt atgtattagc ccaaatttgt
    28141 aaaaataaat gagtaaaaca aaaaaggtca agctattagc ctgacctttt tttatttcgt
    28201 tttataaatg tgtttattat tgttgtggtg atgtttctgc ttttgtagat tcagccttta
    28261 aagttctagt aacagagcct acttctgtat atacatcacc aacaccgttc acagtttcaa
    28321 caacaaatct gtcaacaagt tggtcttgta ttatttgttt atttttcaac attcttaatg
    28381 tccaaatagt tgatgattcg ccattagcat attttctaga agagctaaca gtatttatca
    28441 gttgtggtat tggtgtagct tcatcatctt cactaagttg caataaatat acgaatgttt
    28501 tagatttgtt ttgctctatt actaaagaaa gcctttcttt atttttataa tcatcaatgt
    28561 tagctaagaa gttccatgtt ccactgagtt cgtcttgaac tttgtaaata gttacgtttt
    28621 cattttcagt aattgggtca acttctataa cattatattc atattctaat atttcagtga
    28681 aacgtccatc gctataatat tgtatggtgt ttcttcctgt aactatttta cctttaatat
    28741 caacaacact atctgtacct aaaatctcaa tatttcgact ccagtcttca ccatcaattg
    28801 ttgttacagt aagagtttgg tcaggcttgt tttgataagt ccttttaata gtagattcat
    28861 aattgcttaa aatccaacgt cctgaaactc tcgctttcct agtataaatt gagaaaaaag
    28921 gatcttcgtc gcctttttta aagcaactag tagtgaacag cggaaggata actatcattc
    28981 ctaacaacac taaacttaag tatttttttg catccataat gtatattgct tactttatca
    29041 attttacaaa attaataatt tttaattaaa ttatttacaa ataaatgaaa tatttttcaa
    29101 ataatcctat tttttttcaa aaaaatcata aaaactttca aaaaatcata aaaactttac
    29161 aaaaatagta aaatgtttaa aaaaacccac ttttttgtta ataaattttc ggtttaaaat
    29221 gaaatatttt aaaaattgtt aaaaagtaaa tggtataata ctcaaaatta attaaaattg
    29281 ccttaatttt gtacagattt tataaatttt attatggtaa ttgataaaaa agaaatttct
    29341 gctcgtttaa aacttgtaat tatgctttcg tttttagttt acgcagcaat tttggttatg
    29401 agtttagttt tgggatggtc aaaaactcat ttgtttgaac tttgttggac aatcgttttt
    29461 gtagtgtggt ttatttttgt ttttttgaaa aattacaact acatttggta tagtagtgat
    29521 ggtccaaaaa taattgtacg ttacacttca ttacaacctt tgtcttcagg aaaattttct
    29581 attgaatttc aaaaaaaaga ttttgtaaaa gccgaaattg taaaatcagt tttaggaata
    29641 cgtaaaaatt tagttttgta ttctagaaca cctcaaggag ttgcaaaata tcctaaaata
    29701 aatatttcta ttttaaataa agaggaattg aaaaaaataa aagaagattt gaatatagat
    29761 taaattttta atgctttaga aaacgacttt tgctttgact gattcgcgca tatacgattt
    29821 agccatatct tttttagacg gcattgggga tataaacgcc aagaaattaa tatcatattt
    29881 ttctagtaca aaagcaatat ttgatgcaaa aattaaagaa ttaagctctg ttgcaggtat
    29941 tggagaaatt actgcaaaaa ctctttatgc aagtttcgac aaagccttgc aactggctca
    30001 agaagaacta gaatatattt atacaaatga aattgatttt gtttgttttg gagataaaaa
    30061 ttatcctttt agattaaaag aatgtgatga tgctccaatg gttttatatt acaaaggttt
    30121 gtcggatttt aatgctcaaa aaataattag tattgtaggt acgcgaaaag caacaaatta
    30181 tggaattgac tttgttgaaa ctttaacatc atctattgca agtaaatatc ccgaagctgt
    30241 tattgttagt ggcttggcat acggcattga tgttgcagct cataaatttg cattaaattg
    30301 tgatctaaga acttgggctg ttttgggaca tagtttagag tttatttatc cagcttcgca
    30361 taaatcagtg gcaaaaaaaa tattagaaca agatggctgt attttaagcg attatcctca
    30421 tggacgaaaa attgaccctg ctcattttgc aaaaagaaat agaattgttg caggactatg
    30481 tgatgctctt atagttgttg aatcaggaga aagaggcggt tctatgataa cagcaaatat
    30541 tgcaaatcac tataatcgag acgtgtttgc tttgccaggg agcattaatt cttcttatag
    30601 taaaggctgt aataaattaa ttaaaacaaa cagagccaat ttaatcgagt caatagaaga
    30661 tttggaatat attatgaatt ggtctgacgg aaaaccatct agcttgcaac aaaaattaga
    30721 tttaatttcg gatttgaatg aaaatgaagc taaagttgta aatattttaa aaaaatacga
    30781 atatcttgat atagataact tactacgaca aacaaatatg gataataata ttttgagcct
    30841 ttgtttgttg gagttagagt ttaaaaatat tgtgagagct ttgcctggta aactattttc
    30901 tttaaaaata aaataaatat gttgattgat acacattgcc atttatattt gccagaattt
    30961 gaacaagata ttgatgaaac tattaaaaga gcagaggctc aaagtgtttc aaagataatt
    31021 cttccaaata ttgattcaga aagccttaac ccaatgttta ctctttataa ttccaatgca
    31081 actttgtttt ctacaacaat aggtttacac ccatgttctg ttaaaaaaga ttatgaggag
    31141 gaattgtcaa aaatttttaa agactatact aatattatta atataatagg tataggcgaa
    31201 ataggaatag atttatactg ggataaaact ttttttaaag aacaacaaaa agcatttgat
    31261 tttcaactaa attatgcttg tgagaacgat ttgcctgtga ttattcatag tagaaattcc
    31321 ttaaaagagg ttttagatat tttaaaaatt tatttaccaa aaggacttag aggagttttt
    31381 cattgtttcc cagggaattt agaagaagct aaacaaatag ttgattttgg tttttatttg
    31441 ggcataggtg gaattttaac ctataaaaat aataatatgc tcgaagtagt tgaaaacata
    31501 aatttggaat atatattaac cgaaacagat tctccttatt taagtcctgt ccctcataga
    31561 ggtaagcgta acgaaccggc ttttattatt gaaattgtga aatatatatc taagattaaa
    31621 aatatagatt ttgaaaaagt tgctagtatt acaactaaaa atgccaaaac gctttttaaa
    31681 cttcaataaa ggagaaatct tttacatata taaataggta tattcaaaaa aatagcgttt
    31741 ttttttaaat taattaaaaa aaattttttt aagctaaatt aatttattat tttgcgaaac
    31801 attttgaaag gtcaaaatgg agagatggcc gagttggtcg aaggcgcacg cctggaaagt
    31861 gtgtaggcgg cgtgaaaccg tctcgtgggt tcgaatccca ctctctccgc catattgaaa
    31921 tttgaaataa ttgaattaaa ataaattaaa attaaaattt aaacaaaaaa aatagtcatg
    31981 aaaaaactaa ttgcattatt aactttagca ggaatgctaa catttgcggg ttcatctttt
    32041 atttatgcac aagacgaggc aaaaactgat gaaaacacta cagaacaagt tgaagaaact
    32101 gttgcagaag aagtggcggt tattgacgaa ggagatgctg ctgaagcatc aggagaaaat
    32161 gttccaatgc atcaacaatt gaaagttaaa tttattgaag gtggaccaat gtggatggca
    32221 ccacttataa tttgtttgat tttaggttta gctttagcat ttgagcgtat tatatacttg
    32281 aactttgctt ccacaaatac taaaaaactt ttacttaaag ttgaagaagc tttaaacgaa
    32341 ggtggaatcg aagctgctaa agatgtttgc agaaacactc gcggtcctgt agcaagtatt
    32401 ttttatcaag gcttagatag acatgaagga ggaatagaca tagttgaaaa atcaattatt
    32461 tcttatggtg gcgtacaaat gggattgtta gaaaaaaacc ttacttggat ttctttattt
    32521 attgctatcg cacctatgtt agggttcttg ggaacagttg taggtatgat tatggctttc
    32581 gacgaaatcc aaatagctgg tgatattaac ccatctctag tagcaggagg tatgaaagtt
    32641 gctcttatca ctactgtagc aggtcttatt gttgctatga ttttgcagat tttctataac
    32701 tatattttag gaaaaattga aggaatagtt aatagcatgg aagacgcttc tatttcgttg
    32761 atagacattc tagtagaata cgaagcaaaa caaaaataat ttttaaacaa aaataatatg
    32821 ttagaaaaag tatcaaaaat atttggaatt acattattag tattagtagc actagtaaca
    32881 ctccactttt ttattttcga cctaggtgct ttagaaactc agattgcaga aacagcagat
    32941 ttgccatctg atctaaaagc ctttaaaatg caagaaatag gtataaactg gggtgctact
    33001 atattatatt ttgctattgt tttatttgct ttagcagcta ttttccttgt gggtgcatta
    33061 atttggcaag tagttcgtac aattattgac gataaatcaa aagctcctgt aattttaata
    33121 acacttgcag tgatagtggg aatagtttta ttatgttatt ttgttttcgc ctcaagtgaa
    33181 gtcccatttt tcttaggttc tgataaaata gaagttacag gcgaaacatg taggcttatt
    33241 gatactggtt tatatgtttt atatataact ttaggtttaa caatttgtgc agctatatat
    33301 agcgaaattg ctaaattttg gaaataataa taacgcataa aataattaaa taaattatgg
    33361 cgaaaagaga aacaccagaa gtaaactcag gatcgttggc agacattgct ttcttgttgc
    33421 ttatttactg gcttttaaca acaacaatgg acgtggatac tggtattgct cgtcaattac
    33481 cacctatccc cgaaaaatct gatataacag aagttgatat taataaaaga aatattttta
    33541 tcgtactaat aaactcagaa aatgttctaa gggtaaatgg aaaagaaaga aaaatcgaag
    33601 aactacggga gttgacaaag gaatttgtga aatcaaatcc agacgaccct tctatgcctg
    33661 agctagaatc tgtatatatc gaacagttaa ataaaagagt tgatgtaggt aaaggcgtta
    33721 tttctttgca gaatgataga ggaacatctt atggaactta tattatggtt caaaatgagc
    33781 tagttgctgc agtaaatgaa ctgcgtgacg aagctgctta tgagtatttt ggtaaaaaaa
    33841 gttttgataa cttaagtgat gatcaaaaag aagcagttag aaaaattgtt ccgcaaagaa
    33901 tttcagaagc tgaacctaaa aatattggag gaaaataatt atggcaaaat ttagtggtaa
    33961 aaagaaaaga aaagttggag caataaatac atcttcttta ccagacgtag tttttatgat
    34021 tcttttcttc tttatggcaa caactacaat gagagaagtt gaattgagag tacaagtaaa
    34081 atctagtgat ttaccaaaag ctacagaaat taaaaagcta cagaaaaaat cattggttag
    34141 ttatatctat gtaggaaaac ctttaaaaga atatgttgga atgtatggaa cagaacctag
    34201 aatccaatta aattcttcat tcgccgaatt atccgatatt tacgaatata ttttggctga
    34261 acgcgaaatg agagatgaag ctgaaagacc gtttatgact acctctttaa aagtacatca
    34321 agatactaaa atggggatag taaccgatat taaacaagaa ttgagaaaag cttcagctct
    34381 gaaaattaac tattcggcaa gaaaagaaat ttaataataa tctaaaatat aaaaaaagtg
    34441 caattaactt gcactttttt tatatcttta agtttgagat tttttatttt ttgtatttta
    34501 gcaaccaata taaatgtttt tcgtttacat aaaaaaagaa ttaaaattat gagaaaaata
    34561 atattaataa gttttgttat gttatgtttt ggctttagta aagcacaaac aagcacaacg
    34621 ccagacccaa gattattaga tgtgtattca caagaatatt tggacgcaaa tctagaaaat
    34681 gaaattgaat attttaattg gttcttagac aattcttatt tcgttgcgtt tgtgggatta
    34741 gaaaaggctg aaaccttgcc ttatttatat gaaatagata aggaaactaa ggagaagaaa
    34801 aataaaatat tagcaattga cgaagaaagt tttaacccac tcttaagttc ttatgagatt
    34861 ttttataaca aggaatcggc ttatagaatt ggtaatactg gatatgttat cgctttttat
    34921 tctcaaaaaa aactaactaa aaactataat caatataaaa atgaaaatca atagtttgaa
    34981 aatatttatt ataagcatat ttttgcttat aggtgctact agctttggac agcaatattt
    35041 aattagccaa ggtggaacta ttactacttg tgaaggtact ttgtatgatt cgggtggacc
    35101 aaatggaaat tactcaagta gtgaaaatta tacaataact atatgttctg aagatgggaa
    35161 ttttttagtt ttaaacttta ctcagtttga atttgaatca ggaacttatg ataaattatc
    35221 aatatatgat ggacctaaca ctacaagtcc cgctttagtt actaatgctg gtggtacaac
    35281 tttattaaat caaactttta cttctacagg ttcttgcctt acattgcata tgcaaactga
    35341 tggatctatt actcgtcctg gttttgctgc tgtaatttct tgtttgccac cttgtcaaga
    35401 ttttgaaata gatattatta gtactagcct cccattttct caacctttag attcaatgtg
    35461 gattaatgtt tgtctaggag aaagtattac ttttaccgca gcaggaacat atccgaataa
    35521 taatgttaac tatactcaat ctgatgcaac tactgattgg tattggacat taagttcctc
    35581 aggtcctcct attgaggcaa atggtgtagg catgactact tttactcaca cttttaatga
    35641 atcaggaggt tattttctcg gattggtggg caaagatgct aatggttgtc tagtcagtta
    35701 tttagagcaa attagagtta gagtttcaat tccaccaaat ctttctgttg acccggcagt
    35761 gggtggatgt ataagcgatg caattgagtt atccggacat gtaaccacaa attcttggga
    35821 aattgttatt gctgacgaac aaatagttca agaatgtatt acagatgatc agggagttgt
    35881 gcagtcattt tgttggaata ttaatgcttt tcaacctgga caaaccatta cttcacttaa
    35941 tgacttagaa catgtttgtt taaatattga acactcttgg gtggctgacg tttggatata
    36001 tattgaatgt ccaaatggac aacaggcaga gataaattat tatagtactt ccaacccttg
    36061 ttcaggtgag ttttttggat ctccaaacca ttcagataac tgtgtgccag gtactggttg
    36121 ggattattgc tggaccatga atgcaacaca atcgcataca gcatggtgcc aaggaaattc
    36181 aggctctgtt cctcctggtg attatttacc aacaggtaat ttcgcagatt taataggttg
    36241 tcctataaat ggtgagtggt gtgttataat tattgatgac tggggctctg atgatggaac
    36301 tttgttctct gttgatattg gctttaatca agctgtaact ccttcatact tatggtcata
    36361 ctctcatact tatgctccta caggtatgtc gtggtcgggt aatggcttag gagcaaattc
    36421 tggtggaact gcaacagcat ttccaacaga atcaggcaca caagcatata atttctgtgt
    36481 aacagatgat tttggttgtt cgtattgtat tcctacaact gcagaggttt tgtctataga
    36541 tgatccttct tgttgtattt tacctagtac taatgcagga cttgatgcgg cagtttgttc
    36601 aaatacatat acttttaatg caagtattgc tgcaggaaat actggaactt ggaacatgat
    36661 tagtggacca ggaacagcaa cttgggcaaa tcaaaactct cctcatgcaa cagtaacagt
    36721 aagtgagtgg ggagtgtatg agtttgaatg gatagaagta tatatgaata atccaacttg
    36781 tgcagatact gctactgtta ctattgagtt ttggcccatt ccaacaacaa cttttactta
    36841 tgaccaagta atgtgtaatg cagaccacac aacaataaat tatattggaa acatgtcggc
    36901 aacagctaat tttaattggg attttgatgg agcaacagtt gtaagtggaa gcggaatagg
    36961 accttatact atttattggg cagaaccagg aatgcattat gtaggattaa ccgtttctga
    37021 aaatggatgt tcttcgccag atactttagt taatatttta aatccagcac ttttggaaca
    37081 cagtttagtt gtagataatg atccgtgttt tgcttcgtgt agaggtagag ctgaagtatt
    37141 ccctactgga ggaacagaac cttatgaata ttcttggggt tcacataatc ctgtgttgcc
    37201 aaatttatgt gcaggaactt attctatagt tgttacagat gcaaatggct gtacaacagg
    37261 tcaaaatttt acagttggcg agcctcctga acttgtaata aataacatta cttataatca
    37321 cttaacttgt taccaatcta atgatggaaa tatttccatt gatgctgtag gaggtactgg
    37381 tgagttaact tatcagtggt ctgatatagg ctttggacct gcaaatagag aaaatatatc
    37441 agcacaagaa tattgtgtaa ctgttacaga tgcaaatggt tgctctctta cagattgtat
    37501 aaatattact caaccagcag aattgttagt aactgtttct ccaaatgtag ctatatgcga
    37561 aggaagtcaa actgtacttc aagctaccgg tatgggcgga actgttccat actcttatat
    37621 gtggagatat tctcaagatt ctgcttatat tgtcggtcct tcatcttata tggtaagccc
    37681 agacacaaca cgaatatatg aagtttatgt acgagatgct cacggttgtg aaagtaatgt
    37741 ggcttcaatt aaagttacag ttagtccaga aatgataatc gatactatgt taattaagca
    37801 taatagatgt tatcaatctt gtgatggacg cgccgaaata gttatgcacg gcggattagc
    37861 acctttacat tattcttggg gttcagataa tcatatttat tcaggtttat gtgcaggtat
    37921 ttatacagtt acagtttctg accttattgg ttgtagtgta tcaaatactt ttgtgataga
    37981 acaaccaaca caaattactt actctacatc agtggagcct gcaacttgtt ttggctacga
    38041 cgatggagaa gctactattt ttgttcaagg tggtgtaact ccttatactt acacatggtc
    38101 cacaggacca ggtacagaaa ctattacaac aacagcagga agctataccg ttacagttct
    38161 agatgatcat aattgtagaa ttgtggcaaa tgttgaaatt actcagcctt caaaaattat
    38221 agttttgcca atgagtaata tgagaatttg tcaaactcaa tcggtaagcc ttaataatca
    38281 agctacagga ggaactccat attatgactt taggtgggaa ggcagtgacg gaagtgtttg
    38341 gaactctaat atatatactg taactccaga aacaacaact acttatactt tagtggttac
    38401 tgactctcat ggctgtcata gcagtcctgt tagtacaact atttttgtaa atccaggctt
    38461 aacaataaat tctattgtaa ctagcaatga tactgtttgt cctggcgacc cagcaataat
    38521 tcatgttgat gtggaaggtg gcaatggcgg accatattta atgactttac aagatggacg
    38581 agttgttcct tctccattta tagtttatcc agatacaaca acaatgtatt atattacact
    38641 tgctgatatg tgcggaacac ctacagttat ggattctatt ttaataaatg ctagaccaaa
    38701 accagtaaac ttatttacag ttgaaaaagt tagcggttgt ccaccattcg cagctcagtt
    38761 taatgaactt aatcctcatc tcggacaaac atatttatgg aattttggag acaaaaaata
    38821 ttcaactgag aaaaatccag ttcatattta taaagaacca ggagtttata ctgtaagtct
    38881 tgaagttaga gatgtgtttg gctgtaaatt tacacgtgaa attgaagatt taataacagt
    38941 atatcaaggt cctacagcta actttgaagc atcaccagca atagtgtctt tcataaattc
    39001 agaagttgaa tttataaatc actcttctaa tgcttctaat tattattggt tctttggaga
    39061 tggagacagt tcaatgtttg taagcccacg ccataaatat acagatattg gagattatga
    39121 agtaatgctt gttgctgaaa cagataagta ttgtacagat actactgtta gaacaatagg
    39181 cgttagaaat gaatttgcat tttatatgcc taattcgttt actcctaatg gagacggact
    39241 taacgactgt gctagacctt gtggaaatgg tattgataaa aacgattttt ctttagtaat
    39301 ttatgataga tttggtatgc ttgtttttga aacagataaa tttgacccta aggttggttg
    39361 cgaggcttgt tctgacggag cttgggacgg aacttataaa ggaaataaaa ataagggaga
    39421 taaagttatg gcgaatggtg tgtattactg gtatgccgag tttaaagatt ggtatggaac
    39481 agtacataaa aaacaaggag aaattgtact agttagataa aattaaagag gctgtctcaa
    39541 aacaaatttt aaaacaatga attttcaagg tgtttcagaa gtgttaaaac cctaactccg
    39601 ccaaaatttc acgcttaatt taaaaaaaac ttgataaa
//
LOCUS       DAOPNX010000013        39622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_1340_length_39622_cov_6.860237, whole genome shotgun sequence.
ACCESSION   DAOPNX010000013 DAOPNX010000000
VERSION     DAOPNX010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 39622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39622
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1340_length_39622_cov_6.860237"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PKN53_02555"
     CDS             <1..901
                     /locus_tag="PKN53_02555"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOD87866.1"
                     /translation="FYKGKVRDVYFIGDDILVMVVSDRISAFDVVLPKGIPFKGQVLN
                     QIASKFLDATADIVPNWKIANPDPMVTLGVNCQSFPVEMIVRAYLTGSSWREYKNGAR
                     EICGVKIPEGMREHQAFEKPILTPTTKAEQGAHDENISRDKIISSGLVSASDYLQLED
                     YSLKLFKRGQEIADKMGLILVDTKYEFGKKDGKIYLIDEIHTPDSSRYFYKDTYQERF
                     DKGENQRQLSKEFVREWLMDNGFQGKPGQQIPEMTDEIVNSVSDRYIELFEKITGEKF
                     QKAKSENIENRIFENVSNYLGKL"
     gene            980..1726
                     /locus_tag="PKN53_02560"
     CDS             980..1726
                     /locus_tag="PKN53_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87867.1"
                     /translation="MKSIFEKLNLCRCVSVCSIFVALLFISFSAYSQKAISPKDFEKA
                     HKKLTNSVSGFIKYTYKYTSPEKKLKLNVELFFIKQNNTVKSCYIKQYDDFMLSEYVY
                     YNDTLFSFSNVKNKILSTQNFTLNPFEQRDNKIFVYLIDGSDNYLTAFLQDENYKISK
                     ANLPDYKNILQGESSKKVDFSGVEGTLDTKILLNKKTKCLHSLYQKRDTKNKKSFPVV
                     SEFEINSIKLYNKMNPEVESVIHQKYLERR"
     gene            complement(1755..2468)
                     /locus_tag="PKN53_02565"
     CDS             complement(1755..2468)
                     /locus_tag="PKN53_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOD87868.1"
                     /translation="MTWLERSELLLGANNIEKLKHSKVLVCGLGGVGGAAAEQLARAG
                     IGNLCIVDADTISKSNINRQTIATHDNIGKYKAEELKKRLLSINPELNIDARKVYLRD
                     EILHSTLLEGWDYVVDAIDTLSPKLHLIKTCYQNNIPIVSSMGSGGKSDPSKIKISDI
                     SESYNCGLARNLRKRLHRLGIFNGIKVVFSEELSDKESIIEEEGTNKKSNSGTISYMP
                     VIFGCFCASVVIRSICELD"
     gene            complement(2465..3100)
                     /locus_tag="PKN53_02570"
     CDS             complement(2465..3100)
                     /locus_tag="PKN53_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOD87869.1"
                     /translation="MAFFDFHTHNKNSENAIINIFPNENIISGKLMSCGMHPWHYKEN
                     YADEIIFIEDLAKSKKIIAIGEVGFDPVSQVNLETQNKIFIEHFKISETYNLPLIIHC
                     VKYFHVLKELKKNLNPKQAWIIHSFNSKPEILQDLQRQGFYFSFSNSIFKNSEKAKTI
                     FNTILPERFFLESDDSQLDIKEIYHLASEYFNLEMEELKSQISKNLKHIGI"
     gene            complement(3103..3864)
                     /locus_tag="PKN53_02575"
     CDS             complement(3103..3864)
                     /locus_tag="PKN53_02575"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOD87870.1"
                     /translation="MFFILSKVFQYIFSPVLWIIILFLLAIILKKPKTKKVLFIVGFS
                     MLIFFSQPFIFYEFMRAWEIDAKFKSELKESYDYGILLTGMITYDSKYERINFKSSSD
                     RLWQTMELYKEGYLDKIFIVGGSGEVFNQEFKESQILKDYLIKLGIPEEDILIETNSR
                     NTYENAVEAAKILKPKENNHSYLLITSAFHMRRSNTCFKKQGFECDTYVTDRYAGKRK
                     FTADLLVPKAEILDNWYLLIHEVSGYVIYWITGKH"
     gene            complement(3933..4928)
                     /locus_tag="PKN53_02580"
     CDS             complement(3933..4928)
                     /locus_tag="PKN53_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87871.1"
                     /translation="MTKFYFSIAIFLFLYGNIIAQSSPKNFKDNSINCSYNTLDGKLH
                     GKYISWHKNGQKKSEGQFYKNNRIGTWKVWDNKGNLRCSRKYKNNFEYTSIIPKKTKD
                     ASIKLLNKYPYIPERNKDAYFDLFELEETKAIYSKKYISIILPQDNPILFSKSILELF
                     VANRDSDDFKNYNIFDRFQAKKFEINSLKDLKLIAYKTTKEYVYDLERQIMEARVICI
                     SPILLDTITNTLYDDNWFYLENITPFLAKLPVENFDKKIDVQNISDIFFWKQYSDLEI
                     KNLIYQNEDYKDLDSSVFTDPNKLAELSKDCEKNTLTEIENEHSLWIEFTKNRQK"
     gene            complement(4940..5722)
                     /locus_tag="PKN53_02585"
     CDS             complement(4940..5722)
                     /locus_tag="PKN53_02585"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOD87872.1"
                     /translation="MRLFSKILMLFFIFCFFQNSITAQEFYQIPKQVFDLYEMTGVKA
                     NYIDTVNAFEISQFITLKEYKQFLEDVKADSSKQYYLQMLPDSSIAKTKDVYEKYLNS
                     KKYEKNPVLGVSWDAAVEFCKWKTQKNASSEDFYYSLPLASEWLAAYRYLNKNPIKND
                     FNYKYSDLLLTSVDEMRFALDLNNSNDYEFIFDNVNFHKKDEPRMNRRKRIAGGNYLF
                     EQQTFFNDNYCLFSDKGSEYVSFRIVKVSKNQQNRGISYNQQ"
     gene            complement(5956..8298)
                     /locus_tag="PKN53_02590"
     CDS             complement(5956..8298)
                     /locus_tag="PKN53_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87873.1"
                     /translation="MLKDKHNQFNKLIWFLFAITIVITLAPFFKVGITTADDLSNYTR
                     YLDGTVFSSSWIFARDAGRFYFIITIPIYSLPYVGDSFIFTKIIQNLFLLLSYAMAAI
                     LIKKFFNSREIALLFFLVLVMATPISTNYHIPFIAYPFYFTFSFALLISAVLLFIKYT
                     ETNKYKFLIFSAIIFFISLLFYETYLLYLFFFCIWLFVRNIKLSGGFKNVFKSKTTYK
                     EVVPYASAGLLYVILYFGFRLLLKNEGEIYSGSSFAQNFSWKNFLTVLYELTHTVFPL
                     QIFNLNIDYINRSELSFNSGATSFISILKNIELSTIVNVAIQLVIFIYLFLRLKNISG
                     YKKLGLGILFSLLFAFLSHSALAMSAKYNDTIIATSIKGYVTSYYSYFGVWLAILLFV
                     IIIYKLVKKNKYLKHFTIVIFSIAFAYISIINSYSNRVMARGWEQSQIRFSAIDEMIE
                     KGAFNDCPAEALIFSPDIYWTNVFGYVPDTPDTQGFNWGKYINIKSGPKIDVILSSDR
                     MIEAHNEFPDAKIFRLVKNYTSIENDILFVLAEVDKNSINWDKGKDMFVDATCTRAKV
                     YYYSKSNDFVVQFKTVENANNGFALINSVDLSPISNGINQINVRDITDTGILANFDIE
                     FDVPVYTESFSASNMIPKYSTAIDLIDQTNIKFSKINKIDTEVNVSAENEFPLALIEP
                     ISIDKSYDVISLNAQMEINIANQEIGFAIVIEIRDDKHEIVLWEGYDIKNESEVNFSK
                     LINLVEMRDKNPKLLQLYVWNPNKCEFKINKVKNVSIKAL"
     gene            complement(8444..8812)
                     /locus_tag="PKN53_02595"
     CDS             complement(8444..8812)
                     /locus_tag="PKN53_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87874.1"
                     /translation="MKNLSIKHLLILFLTTMLIVCLTSCEEDFENASTLKKLYKTYKN
                     GSIDECKYNDQTVYRATLNVYDANVVIHDKNGKQIGTCNYAWEQFDVICTKLTDCETI
                     YRCKDNIWGFPEIDKYGLGK"
     gene            9131..10954
                     /locus_tag="PKN53_02600"
     CDS             9131..10954
                     /locus_tag="PKN53_02600"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOD87875.1"
                     /translation="MVANYSEDSIKTLDWEEHIRKRPGMYIGKLGDGKSKDDGIYILL
                     KEVIDNSIDEYAMGFGKNIDIKLEDHTLSVRDYGRGIPQGKLVDVVSKMNTGAKYDSS
                     VFQKSVGLNGVGVKAVNALSSSFKVTSYREGKKKSAEFSRGIKISETELIDTNEPNGT
                     LVVFTPDAEIFEKYNFIQEFIETMLRNYTYLNTGLTINFNGERFYSKNGLLDLLNENL
                     NQEPLYPIVHLKNGDIEIAFTHGTQYSEEYYSFVNGQNTTQGGTHLAAFREAFVKTIR
                     DFYKKDFDANDIRQSIVAAISIRVIEPIFESQTKTKLGSKDMGPEGPTVRNYIFDFMK
                     KVDDFLHKNLDIAEEIQKKILASEKDRKAIAGVQKLARERAKKVSLHNKKLRDCRIHY
                     NSDDERRYETTIFLTEGDSASGSITKSRDVNTQAVFSLRGKPKNSYGETKKIVYQNEE
                     FNLLQAALNIEEGIENIRYRNVVIATDADVDGLHIRLLLITFFLQIFPDLLKHGHLFI
                     LQTPLFRVRNKQETIYCYDEKEKNEAIQKLKNPEITRFKGLGEISPDEFKNFIGKDIR
                     LEPVTLSKADQIQPLLEFYMGKNTPERQEFIINNLRVEEDI"
     gene            10976..13714
                     /locus_tag="PKN53_02605"
     CDS             10976..13714
                     /locus_tag="PKN53_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HOD87876.1"
                     /translation="MSEENNNISNNSSENNEDFVPIEVVEQEDQKVTRLQKMFKDNYL
                     DYASYVILERAVPHINDGFKPVQRRLMHAMKQLDDGRYNKVANIVGHTMQYHPHGDAS
                     IGDALVQLGQKELLIDCQGNWGNILTGDSAAAPRYIEARLTKFALEVVFNPKTTVWKS
                     SYDGRNKEPITLPVKFPLLLAQGVEGIAVGLVSKILPHNFVELIDASIAHLENKDFEL
                     YPDFPTGGYGDFSKYNDGLRGGRARVRAKIEKIDRRTLVIREIPFGTTTGSLIDSIIA
                     ANDSKKIQIRKIEDNTAEFVEILIHLPSGVSSDKTIDALYAFTQCEMSLPTYACVIKD
                     DAPQFIGVSEILKHSVEDTRNLLQQELLIHKAELEEEWHSLSLEKWFIEKRIYKEKEY
                     ENAKSTDQAIDFIHSKAIEHKLNLIREITRDDLLQLLEIKMKRILRFNSDKAEEDLLN
                     ILEEIKKIQYNLEHLTQYTIDYYKRIKEKYGKGRERKTEMRNFDTIDTAQVAISNEKL
                     YVNYKEGFVGYGLKKDEYVCDCSDIDDIIVVRRDGKYIISKVSEKAYFGKDILYVNVF
                     VRNDKRTIYNVAYRDGKTSTSYIKRFAVTGVTRDREYDLTKGAEGSRILYFSANPNGE
                     AEVVKVFLKPKPKIRKLSFEVDFKDIAIKGRTAQGNILSKHDIHKITLKEEGVSTLGG
                     RKIWFDEEIFRLNDSERGLYLGEFEGEDKIIVFTKSGKCRLTNFDLSNHYEENILKIA
                     KYDPDIVYTAIHYDSEQNFFYLKRFQVQEDLRDQLIISETEGSYLVALSYDEFPLVKI
                     VFAGKHESREPELIDAKEFIAVKGIGARGKRLTTFETSEIIFDQPLEKEKPEDEDEEE
                     NFEDDEGEFVQGEEIELEIIDTRDKDEDAVEEKPKKKEDKDKKDPPEGGVQMTLDF"
     gene            13725..14231
                     /locus_tag="PKN53_02610"
     CDS             13725..14231
                     /locus_tag="PKN53_02610"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOD87877.1"
                     /translation="MKIFLMGFMGAGKTVFGKKLAHALNLEFYDLDRLIEQKYKISIP
                     SIFSKFDEGVFRKLETETLKEFENKESFVLALGGGTPCFNNNLEIIKKLGTSIYLKLD
                     AKSLTNRLVNSKTKRPLIKNLNQEELLQKVESMLSEREKYYLQADVVVNGLNLKVEEI
                     INELRIEN"
     gene            complement(14424..16088)
                     /locus_tag="PKN53_02615"
     CDS             complement(14424..16088)
                     /locus_tag="PKN53_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HOD87878.1"
                     /translation="MKTLLITLNAKYIHTSLALRWLYVVNKDKFDISFKEHSIKEDIQ
                     SIANEVIETNCDIVGISVSIWNVDQSKDLCSILKNKNPNLIIILGGPEVSYEPEFFIE
                     NWKIDYVVSGEGEFVLGELLDSLQNNKTAEIPSVSSKQKISKTIAKANIDELIKYPSP
                     YQLEIDKKDVKNRIVYFETSRGCPYHCQYCLSSLETGIRYFPQTYISENLKYIIKSEA
                     KQVKFLDRTFNLNVKHTNFVFDLLLQNYRPNLSFQFEIYADLLNDEIIKFLNESTPEK
                     YFRFEIGIQSTHEPTNIEIKRKQDFQLLAKNCKNLIKGGKIDLHLDLIVGLPYETFEL
                     FVKSFNEVFELGAKEVQLGFLKMLRGTALRNNAPKFDYVYDENPPYQIISNKFISKAE
                     LERIHDAEHALEKFWNSGRFPNTMQRLISQEYKNRYFELFDEIAEFYNKNNLPHFAYQ
                     LEDLFNFLNSFLETKSIDAKPTLRDDYYSNFSARPSGFWCEIMDKKEKKKVLNTISLD
                     ANFLEKNKLSKQILENKTAVYPLENNKYLLSIFENKKIRQVIWQGS"
     gene            16174..17106
                     /gene="rsgA"
                     /locus_tag="PKN53_02620"
     CDS             16174..17106
                     /gene="rsgA"
                     /locus_tag="PKN53_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOD87879.1"
                     /translation="MKRGQVIQSTGANYLVEVDDKIYNCTIRGRLRLADSRETNPVAV
                     GDFVEIEIISEEEGVITEIEERKNCIIRKSTNLSKESHVIAANIDQAVFIHTLKNPET
                     TTVFMDRFLAAAESYSIPPLIIFNKIDIYTREEIEEVAMLMAVYEDIGYKVIPTSIVK
                     NQNLELVKKALKDKVNVIAGHSGTGKSSLINAISPGLNLKTASISKTHLTGRHTTTFA
                     TMHKLSFGGAVIDTPGIRAFGLVYLNKEETGHYFPEIFKMSHNCHFGNCLHINEPNCA
                     VKPAVESGEIAYSRYLSYLNIVLSEEDKHRTKLS"
     gene            17725..18807
                     /locus_tag="PKN53_02625"
     CDS             17725..18807
                     /locus_tag="PKN53_02625"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOD87880.1"
                     /translation="MRTVKILLALFAAVVVFNSCNKEYEAPTITWEPDKMSQLVEMSD
                     ESSLNQALTVTFTAEAGIKDIKIWRNTWLNGAASPTAAIVDGPAISAYEGLTSYEYAF
                     ATNHTLEDFQGGVMKIVYEFEVTDQSEEAQTTTKEYTFFAWETQKVTFVIKDAADTEI
                     TDAKVTLGTESKTAAPYVFYVEDGTYDYTVEKEGYATVTGQVVVNDAAVEETVTLQAN
                     LADWSEKILIAHPSQLGYATYADTRVETASNDVIGVEYKQNNTAGTAAIIVPTANCTG
                     FVVIANDAAYTTSDDLEAAYTAGTAVTQLELGFTYHAPKAYAEKNFIAKVGNEYVWVK
                     YVDGHVAPSATSDGYRGNTLAFQYKK"
     gene            19163..19235
                     /locus_tag="PKN53_02630"
     tRNA            19163..19235
                     /locus_tag="PKN53_02630"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19196..19198,aa:Met,seq:cat)
     gene            19286..20224
                     /locus_tag="PKN53_02635"
     CDS             19286..20224
                     /locus_tag="PKN53_02635"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOD87881.1"
                     /translation="MQQQGLSKKYGFHKTNLIIIFISLFCFLFGQFVSALILHAFDLS
                     IAESLLNHAELSSKEINVLKLAQFFSALLSFVLPSVFCAYLLYDNWKSQLRITKVNYK
                     SSFYLIPLFLLLIMPFMNIIIQWNESIVFPDSLRDLELALRASEDSSQLFIKTLLQGT
                     GVFTLIINILLIGALPALGEELFFRGLLQSHLNDYFKNPHIGIFISAFLFSAIHFQFY
                     GFFPRLILGLIFGYLVYFSGSIWTAVFAHLFNNALAVIVVYFQNLGKISEEVESFGTS
                     SYEIIYSFVCLALAVFVARSLIINFKKRNKIINLVD"
     gene            20242..20985
                     /locus_tag="PKN53_02640"
     CDS             20242..20985
                     /locus_tag="PKN53_02640"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOD87882.1"
                     /translation="MEKLLQQAAETIKKAKYLTVFTGAGISKESGIPVFRGSQGVYSH
                     YDPSVLEITNYFRNTEEAWKGIKAIFYDFIGQAKPNLAHKILAEWESKGLLRAVITQN
                     IDNLHSDAGNKTVIEFHGTTKNFVCTQCSSNFSLNEISITEKPPKCKKCNSLLKPDFV
                     FFGEGIPSQAASQSQMFASQSDVHIVIGTTGEVYPASYIPIYAKNAGAKIIEINPDPS
                     AYTNKITDIYLPMKATEAMEKLDSLVFSV"
     gene            complement(20989..21371)
                     /locus_tag="PKN53_02645"
                     /pseudo
     CDS             complement(20989..21371)
                     /locus_tag="PKN53_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759001.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
     gene            complement(21426..22457)
                     /gene="galE"
                     /locus_tag="PKN53_02650"
     CDS             complement(21426..22457)
                     /gene="galE"
                     /locus_tag="PKN53_02650"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOD87883.1"
                     /translation="MCKIIITGGTGYIGSHTVVEFLESGYQVVILDNLSNSNISILEG
                     IEKITNKKPIFYKVDVCNETEINNILQEHRDAKAVVHFAAYKAVGESVEKPLKYYQNN
                     VGGLVNILTNMLEANIENIVFSSSCTVYGQPEILPVTEQSPIKEAMSPYGNTKQISEE
                     IISDTVKANENLKAISLRYFNPIGAHPSALIGELPIGVPNNLLPFITQTAIGLREKLM
                     VFGNDYNTPDGTAIRDYINVCDLAKAHLVAVERLINKRNTKNHEFFNIGTGKGNSVLE
                     IIKAFEKSTGQKLNYEITSRRAGDIEQVWADTSYAEKVLNWKAETSLEDTVKSAWEWE
                     KSNKSVKSF"
     gene            complement(22468..23760)
                     /gene="tyrS"
                     /locus_tag="PKN53_02655"
     CDS             complement(22468..23760)
                     /gene="tyrS"
                     /locus_tag="PKN53_02655"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOD87884.1"
                     /translation="MNFVEELEWRGMIHDMMPGTKELFEKEFVSAYVGIDPTADSLHI
                     GHLVSVMMLKHLQVAGHRPIALLGGATGMIGDPSGKSQERNLLDEKTLRHNQEAIKKQ
                     LAKFLDFDTPAKTQAVLVNNYDWMSKFSFLEFIRDVGKHITVNYMMAKDSVKKRISGE
                     EKSGMSFTEFSYQLVQGYDFLHLYKEYNCKLQMGGSDQWGNITTGTELIRRKEQGEAF
                     ALTCPLITKADGGKFGKTETGNVWLDQERTSPYAFYQFWLNVADEEAEKYIKIFTLLP
                     KNEINELIKQHKESPHLRILQKKLAEEITVMVHSRKDYEDALEASTILFGNATSDVLK
                     KLDEKTFLSVFDGVPLFNVEKSIIEKGIDIYELLCVNTDIFPSKSELRRTIQGNGLSI
                     NKEKFNDEQTPINSEFLINNKYILVQKGKKNYFLIIAS"
     gene            complement(23764..26112)
                     /locus_tag="PKN53_02660"
     CDS             complement(23764..26112)
                     /locus_tag="PKN53_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOD87885.1"
                     /translation="MKKIFFIASFLIGINISNFSQNLSGENIDIQEYIINLDITDISG
                     KFISGYTDIIFNPIEENINNISLDLLKLNVDSITCNACDISNYTYNDSVLNINFASAF
                     NLEDEISIRVYYNGNPILDPSGWGGFYFVGDYAFNLGVGFKDIPHAYGRVWFPCKDSF
                     TDKAKVTCKITSLENHTAVCGGELINIITNTEDNTKTWEWKLEKPVPTYLVSVAVGPY
                     KLISHNYQGVEKEIPIDFYIHPNDSSKTVNSFANIDTVMAVYENLFGAYPWNRVGYVA
                     VAFNSGAMEHVTNIAMGRGFITGDLTYEDLYYHELAHMWFGNLVTCSSAEDMWLNEGW
                     ATYCETIFREFVKSKENALNFRRSAHEQVLRVYHHKDGGFHPLYPISQDITYSSTVYQ
                     KGASVAHALRGYLGDSVFFTTIKEYLRQNAYTSKSSYDLRDFITNHTGIDLTDFFQDW
                     VFTPGFVHYSIDSTKVSANGSNFDVTVFMKQKLRGRNVFANSNKVPISFMNENFETIT
                     KIIDFDGEFGEQTFTLPFNPMLSLCDYFEQISDASIDETKFINSTGTKNYNNTFFKAI
                     VNSADENDTAMLRITHNWVAPDNFKNEIPGLIIADNRFWTVEGNFPNNFSASAEFAYS
                     NATGSGNLYLDNEFITNSLDSLVLLYRPNRAIDWQIENATNSKFLKKFTVESLKSGEY
                     CLAIYDWEKYLQVENNKIQLTSNIFPNPNNGCFEIALDEKFSGKINIFDINGRLCWGK
                     DFFEENENISISVQNLKSGMYFLNAKSFDGKLFINKKIIIQK"
     gene            complement(26115..27128)
                     /gene="ruvB"
                     /locus_tag="PKN53_02665"
     CDS             complement(26115..27128)
                     /gene="ruvB"
                     /locus_tag="PKN53_02665"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOD87886.1"
                     /translation="MRANSENLSAADKEIEKILRPLEFEDFSGQDKIVANLKIFVQAA
                     KMREEALDHVLLHGPPGLGKTTLAHIIANELGVGFKISSGPVLDKPGDLAGLLTSLDK
                     NDVLFIDEIHRLNPIVEEYLYSAMEDYRIDILIDKGPAARSVQIPLEPFTLIGATTRS
                     GLLTSPLRARFGINSHLKYYTAEVLKKIIIRSAGILNMPIDDDAAGEISRRSRGTPRI
                     ANALLRRVRDFAQVIGDGTIDMKIAKHALEALNIDKYGLDEMDNKILLTIVDKFQGGP
                     VGLKTIATAVAEDEGTVEEVYEPFLIMEGFIKRTPRGREVTEKTYRHLNKINPNTGQQ
                     TLF"
     gene            27737..28525
                     /gene="cdaA"
                     /locus_tag="PKN53_02670"
     CDS             27737..28525
                     /gene="cdaA"
                     /locus_tag="PKN53_02670"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOD87887.1"
                     /translation="MILSFIHIRFLDIIDILLVAFLLYQLYSLIKGTAAIKIFIGIAL
                     IYLVWLFVKAMRMELISSILGQVIGVGLIALIVVFQQEIRQFLLLIGNKYIKRSKLNI
                     LRDSRLQSGYSTSSIEYIAQAANEMSIAKTGALIVISREASLKSFIEQGEVLNADISV
                     PLLKSIFFKDAPLHDGAVLIENNKIYAAKCILPVSANTELPKDFGLRHRSALGMAEVS
                     DTLTVVVSEQTGKISVAKEGKIYRLTTNDRLSKIIANFLRKNDD"
     gene            28646..29926
                     /locus_tag="PKN53_02675"
     CDS             28646..29926
                     /locus_tag="PKN53_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOD87888.1"
                     /translation="MKEINDVIQTKFAVSKKIDESWDFRKANTKELTHCFHSYPAMMI
                     PQVARRIIKNYGKEGDVLFDPYCGTGTSLVEANIKNISAIGTDLNPLARLIATAKTTK
                     INSDLLNSEINNFINYSFKSNFQNKKSYSKIIPDVTNIDYWFSKGTQEKLGTIFGYIE
                     NLGNDSIKNFFKVAFSETVRDVSFVKSGEFKMVRTKNFCHEEDKDVFGIMSSKLERNK
                     LGLEDFSAVCANGTKSAVYGFDTVVGIPNFIDSNSVDIVVTSPPYGDSRTTVAYGQYS
                     RLANEWLGYETANQVDNILMGGRKRKYDHKFDSEILNDAILKIQSKDIQRARDVISFY
                     EDYESSIKNVSKLIKKGGFACYVVGNRTVKGVTIPTDELTAEIFESCGFVHLETIVRN
                     IPSKRMPYRNSPSNIKGKTSSTMKHEYIVVCKKL"
     gene            29929..30630
                     /locus_tag="PKN53_02680"
     CDS             29929..30630
                     /locus_tag="PKN53_02680"
                     /inference="COORDINATES: protein motif:HMM:NF026853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MvaI/BcnI family restriction endonuclease"
                     /protein_id="HOD87889.1"
                     /translation="MRLPDLIKALESLKEEGFIPTKRKCPTGIGHLLENMLGLTETNI
                     AIPDIGGRVELKATRKNANSLITLFTFNRSVWKFSQTEIIKKYGYIDDKGRQALYTTV
                     NNKTPTNQGFILASEPQKNLIVLRNIEEEGNIAEWSTYVLAGKFMTKLDRLLLVFADS
                     KIENNVEHFHFNEAYLLENPTPEKFLDAFETCKLMIDLRMYLKDSGGVRNHGTGFRIS
                     EKNLTELYEKKRRLI"
     gene            30792..33188
                     /gene="amrS"
                     /locus_tag="PKN53_02685"
     CDS             30792..33188
                     /gene="amrS"
                     /locus_tag="PKN53_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOD87890.1"
                     /translation="MNRKAAVAGQFYAGTKESLNQDLKTLFSKAEERKYDARAIISPH
                     AGYVFSGEVAASAINQINPNKKYQNIFIIGSSHTKYFPGVSIYNIGNYETPLGEVKVN
                     IDVCNELIKNNNFISFVPDAHRTEHSIEVQLPFLQYHLKNDFQIVPMILGFDGEQNAK
                     KIAEALKPYWKEENIFIVSTDFSHYPSYNDAVNIDLATANAIVSGKPEELIKVVNSET
                     GIKNLSTNCCAWNSVLSLMYLTDQKYSYNKIQYKNSGDSPYGSDDRVVGYWAISITDK
                     NDSSFSLSDEEKMELLKLARMSAEAKIKENKNLDVSNIVVSENLNEQCGAFVTFNKNS
                     NLRGCIGRFEPNIPLYKVVVEMAVAAATQDYRFSPISAQELDEVEIEISVLTPFKKIK
                     SIDEIELGKHGIYIVKGGRAGTFLPQVAESTGWSLDEFLGYCARDKAGIGYDGWKNAD
                     IFTYEAIVFSESDFKKKDKAKYYEKLEGGRVLCKLCPHECKLSEGKLGFCKARKNVNG
                     ELISLSYGNLVAIHIDPIEKKPLYHFMPGSTSFSIGTAGCNLSCKNCQNSEISQSSPL
                     QLERIKATPEQIVEAAKKNNSASISYTYTEPTVFYEFVLETAKLAKKNGIKNIIISNG
                     FINPEPLKELIPYLDAANIDLKAFDDSIYREITSARLEPILNNLKILRDSGVWLEITN
                     LIIPGHTDDMQMISEMCEWLVENGFAETPLHFSRFFPANKMMHVPPTSVETVEKAAKI
                     AKSKGIKYVYLGNVHQEEGNTICPKCGKTLIKRKTYRTEIDGFSGKCGSCGEVINGVW
                     "
     gene            33270..33941
                     /locus_tag="PKN53_02690"
     CDS             33270..33941
                     /locus_tag="PKN53_02690"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOD87891.1"
                     /translation="MKGQTSSKIELRKMLLAMVIPASFMFLAWMVFALEKSLHISFSE
                     FGLRPRDFKQWYGILTMPFLHGSLEHILANTGSFLILGSMLFYFHNDRAILLFILSYF
                     FSGILTWLIGRESVHIGASAMIYAFAGYLFTAGLIGRNIRRMAVTLIIVFLYGSMVWG
                     MLPINERISWEGHLSGFITGLVLALIFTPPAPPTFSEEDDELDNEEDRYWEIDENKDK
                     DKYLN"
     gene            33944..34894
                     /locus_tag="PKN53_02695"
     CDS             33944..34894
                     /locus_tag="PKN53_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014710219.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOD87892.1"
                     /translation="MQNKQNTKLPWNDYSSYIKRIFGIRVQKISIDAGFTCPNRDGKI
                     SRGACFYCTNESFSPFYCTPKLSVTEQLQKGIEFFSKKYKTQKYLAYFQSYTNTYGKV
                     EDLKKLYLEALAVEGVIGLVIATRPDCIDEEIMKMLKEISKDKYLSIEYGAESTKNET
                     LNFINRGHSWEQTIKAVELANNFQINCGLHFIIGLPGEDKTDFLNHAKKISKLNIQTL
                     KIHQMQVLKKTKLADVYKESPELFYNLNLENYLDLMVEFLGYLNPNVVVERFTSESPK
                     EMIIYPNWKGKKNFEISHMLEKKMKERKITQGMFFEANSI"
     gene            35014..35448
                     /locus_tag="PKN53_02700"
     CDS             35014..35448
                     /locus_tag="PKN53_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOD87893.1"
                     /translation="MRYRKRRHRKLLSPPVIKGFIPYGKDINTENQDLVSMLCEEYEA
                     IKLCDYDMLTHLEASALMGVSRPTFTRIYSSARQKIALAISEGRHLIIEGGKVYYDSH
                     WFHCKDCDAFFTNPNDIVDITCALCASSNVSKVQDEESVSLK"
     gene            35481..35804
                     /locus_tag="PKN53_02705"
     CDS             35481..35804
                     /locus_tag="PKN53_02705"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOD87894.1"
                     /translation="MKIAITSTGTTLGSTIDPRFGRAAYFVIYDIENKSFEICPNPNK
                     EATEGAGPASVQFVAEKGVEKIVSGEFGVKVKDLLDSLKIQMIAIKDHSKTVQEIIDM
                     LNQNK"
     gene            35809..36009
                     /locus_tag="PKN53_02710"
     CDS             35809..36009
                     /locus_tag="PKN53_02710"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOD87895.1"
                     /translation="MPNLDQTGPRGQGAGTGRRAGKCFQGSDSENVNEQNLGQGRGAG
                     LGRRGGRCRGAGRGLGRGLGRN"
     gene            36062..36400
                     /locus_tag="PKN53_02715"
     CDS             36062..36400
                     /locus_tag="PKN53_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOD87896.1"
                     /translation="MKKILAFPLANGVLCSHFGHCEKFAIVEVEDKNIVNIKELTPPE
                     HVPGLYPKWVASFGVTDVIAGGMGQRAIDLFKEQGINAFVGAPIKTAEELAKEFIDGS
                     LELGANYCDH"
     gene            36719..37609
                     /locus_tag="PKN53_02720"
     CDS             36719..37609
                     /locus_tag="PKN53_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD87897.1"
                     /translation="MQENKKNSISIAVASGKGGTGKTMLSLSLFNSIKNKYDDVVLLD
                     ADAEEPNDLLFFDAEEISSTDVTQKIPVIDVNKCKFCGKCRDWCNYNAIFLLASTKFI
                     KLTEELCHGCGACSVACEFGAITEKDASLGSVKNFICDGKKNLYEAMMHQGVMTPVNV
                     IKVAHKITNKHKIRIIDSPPGTSCPFIHAVAEVDYIILITEPTPFGLSDLKQSISTLE
                     SMNKKYGVVVNKAGLGNRAIYDYLSSEGIELLLEIPFDKDIARKYSEGKIISDFKPEL
                     AKDLEKMFDYLLNKYGNSSN"
     gene            37590..38468
                     /locus_tag="PKN53_02725"
     CDS             37590..38468
                     /locus_tag="PKN53_02725"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOD87898.1"
                     /translation="MEIAVISGKGGTGKSSITAAITNALGNVVLADCDVDTANLHLLF
                     NPEIEEEESFVGAETAVIDYGICINCGECMNYCRFDAISKDKNENIVISDIFCDGCGL
                     CEKICPEKAISMLASTDSKLLSGNFRFGKMVLGHLAPGEENSGKLVNLVRDRAREISK
                     TNNIEHIIIDGPPGIGCPVISTVTGVDYCIIVTEPSLSGLHDLKRTIELVESFKLNFG
                     ILINKADLNDKINVKITEFCNQNNYNLLGQINFDTNFVEAMLNTKTIGEMFPESESYG
                     VVKQMVKKVLLSLSES"
     gene            38510..39499
                     /locus_tag="PKN53_02730"
     CDS             38510..39499
                     /locus_tag="PKN53_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOD87899.1"
                     /translation="MEISKTQILSYLEKFKTKRVLIIGDVMIDSYMWGVVNRISPEAP
                     IPIVSVVNREYRMGGAANVAFNIKSLGAEPILCAVVGQDEKGKIFRDLLKQNSMSDEG
                     LLSLPDRKTTIKTRIICGSQHMLRVDDEVDTEIDEKSSEILVEKVEKIIAKQKIDLII
                     FEDYDKGVLNPKFISKVIKIAKKNSIFTAVDPKKRNFNSYNGVDLFKPNFKEFCEGTN
                     SEISKTDLKEIAKSAQKFLESSAISTLLLTLSEHGVFISDIKNNFHFPAERRLIADVS
                     GAGDTAISVAGLLLSAGASLSEIASISNIAGGLVCEKSGVVPIEPEELQNEFR"
BASE COUNT        13757 a         5562 c         6604 g        13699 t
ORIGIN      
        1 cttttataaa ggaaaagtca gagatgtgta ttttattgga gatgacattt tagtaatggt
       61 tgttagcgat agaatttcag cttttgatgt ggttttacca aaaggaattc cttttaaagg
      121 gcaggtttta aaccaaattg cttctaagtt tttagatgct actgccgata tagttcctaa
      181 ttggaaaatt gcaaatcccg accctatggt aacacttggt gtgaattgtc aatcttttcc
      241 tgtagaaatg attgttagag cttatttaac aggttcttcg tggagagaat ataaaaatgg
      301 cgctcgcgaa atttgtggag tgaaaatccc agaaggaatg cgtgaacacc aagcttttga
      361 aaaaccaatt ttgacaccca caacaaaagc agaacaaggt gctcatgatg aaaatatttc
      421 tcgcgataaa ataatttctt cgggtttggt tagcgcaagc gactatttgc aattagaaga
      481 ttattcttta aaacttttta aaagaggaca agaaatagca gataaaatgg gcttaatact
      541 tgttgacaca aaatatgaat ttggcaaaaa agatggtaaa atttacttaa ttgacgaaat
      601 tcatactcca gattcttcgc gttattttta taaagacact tatcaagagc gttttgataa
      661 aggcgaaaat caaaggcaac tttcaaaaga atttgttaga gaatggctaa tggacaatgg
      721 ttttcaaggc aagccaggac aacaaattcc tgaaatgact gatgagattg taaatagtgt
      781 ttctgataga tatattgaac tttttgaaaa aattactggc gaaaaattcc aaaaagcaaa
      841 aagcgaaaat atagaaaata gaatttttga aaatgtgtcg aattatttag gaaagcttta
      901 atcaatttgt tatttttgaa gaaattttaa cgaacttttg atttgaatgt attttgtttt
      961 ataagtaaat aaaattatta tgaaaagtat ttttgaaaaa ctaaacttat gtcgctgtgt
     1021 atcagtttgt tcgatttttg tagctttgct atttataagt tttagtgctt actcgcaaaa
     1081 agcaataagt ccaaaagatt ttgaaaaagc tcacaaaaaa ttgacaaatt ctgtttcagg
     1141 atttataaaa tatacctata aatatacatc gcctgagaaa aaactaaaat taaatgttga
     1201 gttgtttttt ataaaacaaa ataatactgt aaaatcttgc tatatcaaac aatacgatga
     1261 ttttatgcta agcgaatatg tatattataa tgatacttta ttttcttttt caaatgttaa
     1321 aaataaaatt ttaagtactc aaaattttac tctaaatcct tttgagcaaa gagataataa
     1381 aatttttgtg tatttaatag atggtagcga taactattta actgcattct tacaagacga
     1441 aaactataaa attagcaaag caaatttacc agactataaa aacattttac aaggcgaatc
     1501 ttctaaaaaa gtcgatttta gtggtgtaga aggaacatta gatacaaaaa ttttgcttaa
     1561 caaaaaaact aaatgtttac actctcttta ccaaaaacga gatacaaaaa ataaaaaatc
     1621 tttcccagtt gtttctgaat ttgaaataaa ctctatcaaa ttatataata agatgaatcc
     1681 tgaagttgag agtgttattc atcaaaaata tttggaaaga agataaattt tgtataataa
     1741 ttttgctaca aaacttaatc taactcgcat atacttctaa ttaccactga tgcacaaaaa
     1801 cagccaaaaa ttactggcat atagctaatt gttccagaat ttgatttttt gtttgtacct
     1861 tcttcctcta ttatagattc tttgtcgctt agctcttctg agaaaactac ttttataccg
     1921 ttaaaaattc ccaagcgatg tagtcttttt cgcaagttgc gtgctaatcc gcaattgtac
     1981 gattctgaaa tatctgaaat ttttattttg cttgggtctg actttcctcc tgaacccata
     2041 gaacttacaa taggaatatt gttttgataa caagttttta ttaaatgtag ttttggcgac
     2101 aaagtatcaa tggcatcaac aacataatcc cagccttcga gtagagtgct atgtaaaatt
     2161 tcatctctta aataaacttt acgagcatca atatttagtt cgggatttat gctgagcaag
     2221 cgttttttta attcttcggc tttatatttt ccaatattgt cgtgggtagc aatagtttgt
     2281 cgattaatat ttgatttgct aatggtgtcg gcatcaacta tacataaatt tccaattcca
     2341 gctctggcaa gttgttcggc agccgcacca ccaactccgc caagtccaca cactaaaact
     2401 ttggaatgtt ttaatttttc aatattgttt gcacctaaaa gtagttcgct acgctctaac
     2461 caagtcatat gccaatgtgt ttaaggtttt tactaatttg agattttaat tcttccattt
     2521 ctaaattaaa atactcgctt gcaagatgat agatttcttt tatatctaat tgagaatcat
     2581 cgctttctaa aaaaaatctc tcaggcagaa tagtgttaaa tattgttttt gctttttctg
     2641 aatttttaaa aatagaattt gaaaatgaaa aataaaatcc ttgtctttgc aagtcttgta
     2701 aaatttctgg tttagaatta aaactgtgga taatccatgc ctgctttggg tttagatttt
     2761 ttttcaattc ttttaaaaca tgaaaatatt ttacacaatg aataattaat ggaagattat
     2821 aagtctcgct aattttaaaa tgttctataa aaattttatt ttgagtttcc aaatttactt
     2881 gagacacagg atcaaaacct acttcgccaa tggctataat tttttttgat tttgctaaat
     2941 cttcaataaa aataatttcg tctgcataat tttctttata gtgccatgga tgcataccgc
     3001 aagacatcag tttgccagaa ataatatttt cgtttggaaa aatattgatt atagcatttt
     3061 cagaattttt gttatgtgta tgaaaatcaa aaaaagccat ttttagtgtt ttcctgtaat
     3121 ccaataaatt acatatcccg aaacttcgtg aataagtaag taccaattat ctaaaatttc
     3181 tgctttaggg actaataaat cagctgtaaa ttttcttttt cctgcatatc tgtcggtaac
     3241 ataggtgtcg cattcaaagc cttgtttttt aaagcaagta tttgacctac gcatgtgaaa
     3301 agcagaagta atcagtaaat atgaatgatt gttttcctta ggtttaagga tttttgcggc
     3361 ttcaacagca ttttcgtaag tgtttcttga gtttgtttct attaaaatgt cttcttcggg
     3421 aatgccaagt tttatcaaat aatctttaag aatttgcgac tccttaaatt cttgattaaa
     3481 aacttcgcca gaaccaccaa caataaaaat tttatctaaa tatccttctt tatacaattc
     3541 catggtttgc catagcctgt cagacgaaga tttaaagttt attctttcat atttagagtc
     3601 ataggtaatc attccagtga gtaaaatgcc ataatcgtag gattctttta attcagattt
     3661 gaattttgcg tctatttccc aagctctcat aaattcataa aaaataaaag gctgtgagaa
     3721 aaatattagc atagaaaatc caactatgaa caaaactttt ttagttttag gtttttttaa
     3781 aattatagca agcaaaaaca atattattat ccataaaact ggagaaaata tatattgaaa
     3841 tacttttgat aaaataaaaa acatatagat ttttaatttt tttgtaaaaa taggtaaaaa
     3901 tagatatgtt ttaaaaaatt agaggaataa atttactttt ggcgattttt agtaaattct
     3961 atccataaag aatgttcatt ttcaatttcg gttaaagtgt ttttttcaca atctttagat
     4021 agttctgcta acttgtttgg gtctgtgaaa acgctagaat ctaaatcttt ataatcttca
     4081 ttttgatata tcaagttttt aatttctaag tcgctatatt gtttccaaaa gaaaatgtca
     4141 gaaatatttt gaacatcaat ttttttgtca aaattttcaa caggaagttt tgctaaaaat
     4201 ggagtgatat tttctaaata aaaccaatta tcgtcgtaaa gtgtgtttgt aatagtgtca
     4261 agcaaaatag gggaaatgca tattactctt gcttccatta tttgtctttc caaatcataa
     4321 acatattctt tggttgtttt atatgcaatt aatttgagat cctttaaaga atttatctca
     4381 aactttttcg cttgaaatct atcaaaaata ttatagtttt taaagtcatc agaatctcga
     4441 tttgcaacga aaagttctaa aatagatttg ctaaaaagaa taggattatc ttgtggcaaa
     4501 ataatgctga tatacttttt actataaatt gcttttgttt cttctaattc aaacaaatca
     4561 aaatacgcat ctttatttct ttcaggtata tatggatatt tattgagcaa ttttatagaa
     4621 gcatctttgg tttttttggg gattatagac gtatattcaa aattattttt gtatttcctt
     4681 gaacatctca aatttccttt attgtcccaa actttccatg tgccaattct attgttttta
     4741 taaaattgac cttcagattt tttttgtccg tttttgtgcc atgaaatata ttttccgtgt
     4801 aattttccat ctaaagtgtt gtaagagcaa ttaatggagt tgtctttaaa gttttttgga
     4861 ctgctttgtg cgattatatt tccgtacaag aaaagaaaaa tagctattga aaaataaaat
     4921 tttgtcataa aatgttgatt tattgttgat tataagaaat gcctctattt tgttgatttt
     4981 tgcttacttt cactattcta aatgacacat attcagagcc tttgtcagaa aataggcaat
     5041 aattgtcatt aaaaaatgtt tgttgttcaa ataaataatt accacccgca atacgttttc
     5101 gtctattcat tcttggctcg tcttttttgt gaaaattgac attgtcaaag ataaattcgt
     5161 aatcattact attgtttaaa tctagtgcaa atctcatctc atcaacagat gtaagcaaca
     5221 aatctgaata tttataatta aaatcgtttt ttattggatt tttgtttaaa tacctataag
     5281 ctgctaacca ttctgaagcc agaggtaagc tataataaaa atcttctgaa gaagcatttt
     5341 tttgtgtttt ccatttgcaa aattctacag cagcgtccca gcttactcct aaaacaggat
     5401 ttttttcata cttttttgag tttaaatatt tttcataaac atcttttgtt ttagcaatgg
     5461 acgaatcggg aagcatttgt aaataatatt gctttgatga atcggctttt acatcttcta
     5521 aaaactgttt gtattctttt aaagtaataa actggctgat ttcaaatgca ttaacagtat
     5581 caatataatt tgcttttaca cctgtcattt cgtacaaatc aaaaacttgt ttgggaattt
     5641 gataaaattc ttgtgcagtg atagaatttt gaaaaaaaca aaatatgaaa aaaagcatta
     5701 aaattttgga aaataatctc atattatttt agtttttgtg taatagttgt gcaatatcta
     5761 tatgttaacg tgatttttta gtttttagtg tttagttttt agttaaataa ctttaaaaac
     5821 tttagtcaag gtttggaata taataaaaca atatttcaat gattttttat tttgcttatt
     5881 atgagcctgt tatggctctc catggaattt atgccacagt ctgtggcaca aattatagaa
     5941 tttctattca cattgctaga gagcctttat actcacgttt ttaactttat tgattttaaa
     6001 ttcacattta tttggattcc aaacatatag ttgtagcaac tttggatttt tgtctctcat
     6061 ttctacaagg ttaatcaact ttgaaaagtt tacttctgat tcatttttaa tatcatagcc
     6121 ttcccaaagt acaatttcat gtttatcatc gcgaatttca atcacaattg cgaaaccaat
     6181 ctcttgatta gctatgttaa tttccatttg tgcattcaac gaaattacat cataactttt
     6241 gtctatgctt attggctcaa ttaaagccaa tggaaattcg ttttcagcac ttacatttac
     6301 ttctgtgtcg attttattta ttttagaaaa tttaatatta gtttggtcaa ttaaatctat
     6361 tgcagttgag tattttggaa tcatatttga agctgaaaaa ctttcggtat aaactggtac
     6421 atcaaattca atgtcaaaat ttgcaagtat tccagtatcg gtaatatctc tcacattaat
     6481 ttgatttata ccattagaaa taggagataa atcgacacta tttattagtg caaaaccatt
     6541 attggcattt tccactgttt taaattgaac aacaaaatca tttgattttg agtaataata
     6601 aacttttgcc cttgtacaag ttgcatcaac aaacatatct ttgcctttat cccaatttat
     6661 gctgttttta tcaacttctg caagaacaaa aagaatgtca ttttcaatcg aagtataatt
     6721 tttaactaat ctaaagattt ttgcatcggg aaattcatta tgtgcttcaa tcattctatc
     6781 cgaggataat ataacatcaa tttttggacc tgattttata ttaatatatt taccccaatt
     6841 aaagccctgt gtatcaggag tgtctggcac ataaccaaaa acgttagtcc aatatatgtc
     6901 aggagaaaag attaacgctt cagcaggaca atcattaaac gctccttttt caatcatctc
     6961 atcaattgct gaaaacctaa tctggctttg ttcccagcct cttgccatta ctcgattcga
     7021 ataagaatta ataatggata tgtaagcaaa tgcaatagaa aatataacaa ttgtaaagtg
     7081 ttttaaatat ttgtttttct ttactaattt gtaaataatt atcacaaata gtaaaatagc
     7141 taaccaaact ccaaaatatg aatagtagga agtaacgtaa cctttaatac ttgtagcaat
     7201 gattgtgtca ttatatttag cagacattgc aagagccgaa tgagataaaa aagcaaacaa
     7261 taaagaaaat aaaattccta atccaagttt tttatatccc gaaatgttct taagtctgag
     7321 gaaaagataa atgaaaatta ccaattgaat agcaacattt actatagtgc ttaattcaat
     7381 atttttaaga attgatataa atgaggtagc tccagagtta aaagataatt ctgatcggtt
     7441 tatataatct atgtttagat taaatatctg caatggaaaa acagtatgcg taagttcgta
     7501 aagcacagtt aagaagtttt tccaactaaa attttgtgca aatgagctac cagagtaaat
     7561 ttctccttca tttttaagta gcaatctaaa accaaaatat agtattacat acaaaagccc
     7621 tgctgatgca tagggaacaa cttctttgta tgttgttttt gatttgaaaa catttttaaa
     7681 acctcctgac agtttgatgt tgcgtacaaa taaccaaata cagaagaaga ataaataaag
     7741 caagtaggtt tcgtaaaata acaatgaaat aaagaaaata attgccgaaa aaattaggaa
     7801 tttgtactta tttgtttctg tatatttgat aaacagcaag actgctgaaa ttaacaaagc
     7861 aaagctaaaa gtaaaataaa atggataggc aataaaagga atatgataat ttgtcgaaat
     7921 aggagtagcc attaccaaaa ctaggaaaaa aagaagtgct atctcgcgag aattgaaaaa
     7981 ttttttaatc aaaattgctg ccatagcgta agagagtagc aagaacaaat tttgaattat
     8041 tttagtaaaa ataaaactat cgcccacata tggtagcgaa taaattggta ttgtaataat
     8101 aaaataaaat ctgcctgcat ctcttgcaaa tatccatgaa cttgaaaaaa ctgtgccatc
     8161 aagatatctt gtgtagttgc ttaaatcatc agcagttgta atccctacct taaaaaaagg
     8221 agctaatgta ataacaattg taattgcaaa tagaaaccaa ataagtttat tgaattgatt
     8281 atgtttatct ttaagcattt taaaatacct tataaattat gttgcaaata taacagttta
     8341 attttcaaaa caagtatttg taaacttatt ttacatgaaa gcattttgta gtgaggaaaa
     8401 atattttaca aagtaaactt aaagtttttt taatgtttct atattacttc ccaagtccat
     8461 atttatcaat ttcgggaaat ccccaaatat tgtctttaca tctgtaaata gtttcgcagt
     8521 ctgttagttt tgtgcatatt acgtcaaatt gttcccaagc gtaattgcaa gttccaattt
     8581 gttttccgtt tttatcgtga ataacaacat ttgcgtcata aacgtttaat gttgcacgat
     8641 aaacagtctg gtcattgtat ttacattcgt cgattgaacc atttttgtat gtcttatata
     8701 gctttttgag tgtgcttgca ttctcgaagt cttcttcaca acttgttaaa caaactatta
     8761 gcattgttgt caagaataaa atcaataaat gtttgatact taaatttttc attgcagttc
     8821 ttaaataaaa ttatacgaat tttatcgaaa taagtttcgt ttttgcttac aatgtggtga
     8881 ggattttaaa acttgaacta atttatgctg aaattcttac gtttcatatc acctacactt
     8941 atatcattgt tctacgaata aattgttaat aaatttaaag tactgcattg gtattttaag
     9001 tgtttgtaat gtgtgtgtga aatatcatat cttttttatc aactttaaaa tgttaatatt
     9061 ttaatttgca aaatttgaaa aaataaatat attaaagcta tttttgtgca cttatggaaa
     9121 ggactgttaa atggtagcga attattcaga agatagtatc aaaactttag attgggaaga
     9181 acatatccga aaacgcccag gaatgtatat aggcaaatta ggagatggga agtccaaaga
     9241 tgatggaatt tatattttgc ttaaagaagt tatagacaac tctattgacg agtatgctat
     9301 gggctttggg aaaaatatag atattaagct agaagatcac actctttctg tgagagacta
     9361 tggacgtggt attccgcaag gaaaattagt agatgtggtt tctaaaatga acacaggagc
     9421 aaaatacgat tctagcgttt ttcaaaaatc tgttggactg aatggagtag gagttaaagc
     9481 tgtaaatgct ctgtcttctt cttttaaagt tacttcatac cgcgaaggaa aaaagaaatc
     9541 tgctgaattt tctagaggaa taaaaatttc tgaaacagaa ctaattgata caaatgagcc
     9601 aaatggtact ttagtcgttt ttacacctga tgcagagatt tttgagaaat ataatttcat
     9661 tcaagaattt atagaaacta tgttgagaaa ttatacttat cttaacacag gtttaactat
     9721 aaactttaat ggtgaacgtt tctattcaaa aaatggctta ctagatttgc taaacgaaaa
     9781 tcttaatcaa gaacctctgt atcctatcgt gcatttaaaa aatggcgata tagaaatagc
     9841 ttttactcac ggcacacaat atagcgaaga gtattattct tttgtaaatg gacaaaatac
     9901 aactcaaggt ggtactcact tggctgcttt tagagaagct tttgtgaaaa ctattcgcga
     9961 cttttataaa aaagattttg atgctaatga tattagacaa agtatagtag ctgcaataag
    10021 tataagagtt atagaaccta tttttgagtc acaaacaaaa acaaaattag gctcaaaaga
    10081 tatgggtccc gaaggtccta ctgtgagaaa ttatattttt gactttatga aaaaagttga
    10141 tgactttctt cataaaaacc ttgatattgc tgaagaaatt caaaagaaaa ttcttgcttc
    10201 ggaaaaagat agaaaagcaa ttgcaggagt tcaaaaatta gctagagaaa gagcgaaaaa
    10261 agtaagtctt cataataaaa aactaagaga ttgtagaatt cattacaatt cagacgatga
    10321 aagacgttac gaaacaacaa ttttcttaac cgagggcgat tctgcaagcg gttctattac
    10381 aaagtcaaga gatgtaaata ctcaagctgt ttttagtttg agaggaaaac ctaagaattc
    10441 ttatggagaa actaaaaaaa tagtttacca aaacgaagaa tttaatcttt tgcaagctgc
    10501 tttgaatata gaagaaggaa ttgaaaacat aagatataga aatgtagtga tagcaaccga
    10561 tgccgatgtt gacggtttgc atattcgtct tttgctaatt acttttttct tgcaaatttt
    10621 tccagattta ttaaaacacg gacatttgtt tattttgcaa acaccgcttt ttagagttag
    10681 gaataaacaa gaaacaattt attgctatga cgaaaaagaa aaaaacgaag ctattcaaaa
    10741 acttaaaaat cctgaaataa ctcgttttaa agggctaggt gaaatttcac ctgacgaatt
    10801 taaaaatttt ataggtaaag acattagact tgagccagta actttgtcga aagccgacca
    10861 aattcagcct ttacttgagt tttatatggg gaaaaacacc cctgaacgtc aggaatttat
    10921 tattaacaat ctaagagttg aagaagatat ataaaaagga agatttttat aaaacatgag
    10981 tgaagaaaat aacaatatat ctaacaatag ttcagaaaat aacgaagatt ttgttccaat
    11041 tgaagttgta gagcaagaag accaaaaagt tactcgtctg caaaagatgt ttaaagataa
    11101 ctatttggat tatgcttctt acgtaatttt ggagcgtgca gtacctcata taaatgacgg
    11161 ttttaagcca gttcaaaggc gtttgatgca tgccatgaaa caactcgatg atggacgata
    11221 taataaagtt gcaaatattg taggtcatac aatgcaatat cacccacatg gagacgcttc
    11281 tataggcgat gctcttgtgc aacttggaca aaaagaactt ttaattgatt gtcaaggaaa
    11341 ctgggggaat attttaaccg gcgactcagc tgcagcacca cgatatatag aagctagatt
    11401 aacaaaattt gctcttgaag ttgtttttaa tcctaaaact acagtttgga aatcatcata
    11461 cgatggaaga aataaagaac ccataacttt accagtaaaa ttccctttgc ttttagctca
    11521 aggagtagaa ggtatagctg tgggcttagt ttcaaaaatt ttaccacata actttgtaga
    11581 acttattgat gctagcattg cacatttaga aaataaggat tttgaattat atcccgattt
    11641 cccaacaggt ggatatggag attttagcaa atataatgat ggattaagag gcggacgtgc
    11701 tagagtgcgt gcgaaaatcg aaaaaataga tagacgtaca ttagttattc gcgaaattcc
    11761 gtttgggaca acaacaggtt cgttaattga ctctattatt gctgccaacg acagtaagaa
    11821 aattcaaatt aggaaaattg aagataatac agcagaattt gttgaaattt taattcactt
    11881 gccatcagga gtttctagcg ataaaactat tgatgcttta tatgcattta cacagtgcga
    11941 aatgtcgcta cccacatatg cctgtgttat aaaagatgat gcaccgcaat ttataggtgt
    12001 ttccgaaatt ttaaagcatt cagttgaaga tacaagaaat cttcttcagc aggaattatt
    12061 aattcataaa gctgaactag aggaagaatg gcactcgctt tctttggaaa aatggtttat
    12121 cgaaaaacgt atttataaag aaaaagaata cgaaaatgca aagtcaacag atcaagcaat
    12181 tgattttatt cattcaaaag caatagaaca taaattaaac ttaatacgcg aaattacgcg
    12241 agacgattta ttacagttgc tagaaataaa aatgaagaga attttgcgtt ttaatagcga
    12301 caaagccgaa gaagatttgt tgaatatttt ggaagaaatt aaaaaaatcc aatataatct
    12361 tgagcatctt actcaataca ccattgatta ttataaacgt attaaagaaa aatacggtaa
    12421 gggtcgcgaa cgcaaaactg aaatgcgaaa tttcgatact atagatactg cacaggttgc
    12481 aatatctaat gaaaagcttt acgtgaacta taaagaaggc tttgtaggtt atggtttgaa
    12541 aaaagatgaa tatgtttgcg attgttctga tattgatgac attatagttg ttagaagaga
    12601 cggaaagtat ataatctcaa aagtttctga aaaagcatat tttgggaaag atattttgta
    12661 tgtaaatgtt tttgttagaa acgataagcg aacaatttat aatgtagctt atagagatgg
    12721 taaaacttcc acttcttata taaaacgttt tgctgtaaca ggagttactc gcgatagaga
    12781 gtatgattta acaaagggag ccgaaggttc gagaattctt tatttttctg caaatccaaa
    12841 tggagaagcc gaagtggtaa aagtattttt aaagccgaaa ccaaaaatta gaaagctttc
    12901 ctttgaagtt gattttaaag atattgcaat aaaaggacgt actgcacaag gaaatatact
    12961 ttctaaacat gatattcata aaattacttt gaaagaagaa ggtgtttcta cacttggtgg
    13021 caggaaaatt tggtttgatg aagaaatttt tagacttaat gatagcgagc gagggttgta
    13081 tttaggtgag tttgagggcg aagacaaaat tattgtcttt acaaaatctg gaaaatgtag
    13141 attaacaaat tttgatttat ctaatcatta cgaagaaaat attttaaaaa ttgcaaaata
    13201 cgatccagac atagtttata ctgctattca ttacgattct gaacagaatt tcttctatct
    13261 aaaacgtttc caagttcaag aagatttacg tgatcaattg ataatttcgg aaactgaagg
    13321 ttcttatctt gttgctcttt cttatgatga atttccattg gtaaaaatag tttttgcagg
    13381 taagcacgaa tcacgtgaac cagaacttat tgatgctaaa gaatttattg ctgttaaagg
    13441 aattggtgct agaggaaagc gattaacaac ttttgaaact tctgaaatta ttttcgacca
    13501 gcctttggaa aaagaaaagc ctgaagatga agatgaagaa gaaaattttg aagatgatga
    13561 aggcgaattt gtacaaggcg aagaaattga acttgaaata attgacacta gagataaaga
    13621 cgaagatgcc gttgaagaaa aaccaaagaa aaaagaagat aaagataaaa aagacccacc
    13681 agaaggcggt gtgcaaatga ctttggattt ttaaaatcta aattatgaaa attttcttaa
    13741 tgggttttat gggtgcaggc aaaacagttt ttgggaaaaa gcttgcccat gctttaaatc
    13801 tcgagtttta tgatttagac agacttattg agcaaaaata taaaataagt attccaagta
    13861 ttttttcaaa atttgatgag ggagttttta gaaaattaga gacagaaact ttaaaagaat
    13921 ttgaaaataa agaaagtttc gtcttagcac ttggcggtgg tacaccatgt tttaataata
    13981 atttagaaat aataaaaaaa cttggcacta gcatttattt aaaattagat gcaaaatctt
    14041 taacaaatcg cttagtaaat tcaaaaacca aaagaccatt aataaaaaat ctaaatcagg
    14101 aagaactttt gcaaaaagtt gaaagcatgc tcagcgaaag agaaaaatat tatttgcagg
    14161 ctgatgttgt tgtgaatgga ttaaatctta aggttgagga aataattaat gaattaagaa
    14221 ttgagaatta atgaattaag aattgagtta aaagtttata aagttagtaa agtttttaaa
    14281 gaaagccaat cccgcaagcc cctttcaact ttcaacactt caactacgtt cagtgcatcg
    14341 ctttcggctt tcaactttac cttcggtgag ctcgcaagct cggttcaaca cttcaactac
    14401 gttcagtgca tcgcttttaa cttttaactg ccttgccata ttacttgtct aattttttta
    14461 ttttcaaaaa tgcttagcag atatttgttg ttttctaagg gatagactgc ggttttgttt
    14521 tctaaaattt gttttgaaag tttatttttt tctaagaaat tggcatctaa agaaattgta
    14581 tttaaaactt ttttcttttc ttttttatcc ataatttcac accaaaatcc tgaaggacga
    14641 gcagaaaaat ttgagtagta atcgtctcgt aatgtgggtt ttgcatcaat gctttttgtc
    14701 tctaaaaaag aattgaggaa attaaacaaa tcttcgagtt gataagcaaa atgtggcaaa
    14761 ttatttttat tataaaattc agcaatttcg tcgaaaagtt caaaatatct atttttatat
    14821 tcttgactta ttagtctttg catagtgttt ggaaatctgc cactattcca aaatttttct
    14881 aaagcatgtt cagcatcatg gattctttca agctcagctt ttgaaataaa tttattgcta
    14941 attatttgat aaggtggatt ttcatcatat acataatcaa atttgggtgc attatttctt
    15001 aaagcagttc cacgcaacat ttttaaaaat ccaagctgta cttcttttgc acctaactca
    15061 aagacttcgt taaaagattt tacaaaaagt tcgaaagttt cgtatggcaa gccaacaatc
    15121 aaatctaaat gtaagtcaat ttttccgcct ttaattaaat ttttgcagtt ttttgcaagt
    15181 agttgaaaat cttgtttgcg tttaatttca atatttgtag gttcgtgtgt ggattgaata
    15241 ccaatttcaa atctaaaata tttctcggga gtactttcgt ttaaaaattt tattatttca
    15301 tcgtttaata aatcggcata aatttcaaat tgaaaactta aatttggtct ataattctgc
    15361 aaaagtaaat cgaaaacaaa atttgtgtgt tttacattca aattaaaagt acggtctaaa
    15421 aattttactt gtttagcctc actttttata atgtatttta agttttcaga aatatatgtt
    15481 tgtgggaaat atctaatacc agtttccaaa gacgacaaac aatattggca gtgataagga
    15541 cagcctcgcg aagtttcaaa ataaactata cgatttttta catctttttt gtctatctcc
    15601 aattgatagg gcgaaggata ttttataagc tcgtcaatat ttgctttagc aattgtttta
    15661 ctaatttttt gttttgaaga aactgatggg atttctgccg ttttattatt ttggagagaa
    15721 tcaagcaatt cgcctaagac aaactcgcct tcgccactta caacataatc aattttccaa
    15781 ttttcaataa aaaattcggg ttcgtaacta acttcgggtc cgccaagtat tataattaaa
    15841 ttcggatttt tattttttaa aattgaacac aaatctttac tttggtcaac attccagatt
    15901 gaaacgctta ttccaacaat atcgcaatta gtttctataa cttcgtttgc aatgctttga
    15961 atatcttctt ttattgaatg ttctttaaaa gaaatgtcaa atttatcttt atttacaaca
    16021 taaagccagc gcaaagccaa agatgtgtgg atatattttg cgtttaatgt tataagtaaa
    16081 gttttcattt tacaaagata tgcaaattca aatatacaaa attcgttaca acaatatttg
    16141 caatattatt tttttatata aatttgcgtg catatgaaaa gaggacaagt aatacaaagt
    16201 actggggcta attatttagt cgaagttgac gataaaatct ataattgcac tatcaggggg
    16261 cgacttaggc ttgcagattc gcgagaaaca aatcctgttg cagttggcga ttttgttgaa
    16321 attgaaataa tttcagaaga agaaggagtg attactgaaa ttgaagagcg taaaaattgt
    16381 attattagaa aatcaacaaa tctatctaaa gaatctcatg taattgcagc aaatattgac
    16441 caagcagtat ttattcacac tttaaaaaat ccagagacaa caacagtttt tatggataga
    16501 tttcttgcag ctgctgaatc ttacagtatt ccaccattga ttatttttaa taaaatagat
    16561 atttacacaa gagaggaaat tgaagaagta gctatgttaa tggctgttta tgaggatatt
    16621 ggatataaag taattcctac ctctatagta aaaaatcaaa atttggagct tgttaaaaaa
    16681 gcattgaaag ataaagtaaa tgtgatagca gggcatagtg gaacaggaaa atcttcgtta
    16741 ataaatgcaa tttcgccggg tttaaattta aaaactgctt caatttcaaa aactcacctt
    16801 acaggaagac atacaaccac ttttgcaaca atgcacaaac tttcttttgg tggagctgta
    16861 atcgacactc caggaattag agcttttggt ttagtgtatt taaacaaaga agaaacagga
    16921 cattattttc ctgaaatttt taaaatgtca cataactgtc attttggcaa ctgtttgcat
    16981 ataaacgaac ctaattgtgc tgtaaaaccc gctgtagaaa gtggagaaat tgcgtattca
    17041 aggtatttgt catatttgaa tattgttttg agcgaggagg ataagcatag gacaaaactc
    17101 agttaaaagc gatgcactga acgtagttga agtgttaaag gttaaaagcg atgcactgaa
    17161 cgtagttgaa gtgttaaaag tttgattctg aatgaattat gcagtatagt gtagagacgg
    17221 tgcatgcgcc gtctctacac tatacaaatt ttaatttatt ataccacaaa aaattagttg
    17281 aaagttcata aagtaaaccg agtttacgag ctcgctgaaa gcaaaagttt taaaagaaaa
    17341 gccaaacacg taggctactt ttaactttta gttcaaattt agttgtaata ggcgatgtgg
    17401 ggtggtgttg gtaataaaac aaaagtttgg cacagattat gcaagtatag aagttagact
    17461 tataaaaagt taaaaattaa taattaacta acataagcaa gttctttgaa actacattat
    17521 taacattcct taaaataaca gaaaacaagg tcaaaaggct tcatttgttg attttttgaa
    17581 taacaaattt tgtttttaat aaaattttta gtattttgtg ttgttattat aaaataatgt
    17641 gtaaatttgc atagaattta aattttaaat tagatagata ataaaaatta aaaagaagtt
    17701 taactaaaat taataaaata aattatgaga acagtaaaaa ttttattggc attatttgcc
    17761 gcagtggtag tttttaatag ttgtaataaa gagtatgaag ctccaacaat tacttgggaa
    17821 ccagataaaa tgtcacaatt ggttgaaatg tctgatgaat cttcattaaa tcaagcttta
    17881 actgttactt ttacagcaga agcaggtatt aaagacatta aaatttggag aaatacttgg
    17941 ttaaatggtg cagcatctcc tacagctgct atagtagatg gcccagcaat ttcagcttat
    18001 gaaggtttaa caagttatga atacgctttt gcaacaaatc acactcttga agattttcaa
    18061 ggtggagtaa tgaaaattgt ttatgaattt gaagttacag accaaagcga agaagctcaa
    18121 accacaacta aagaatatac tttctttgca tgggaaactc aaaaagttac ttttgttata
    18181 aaagatgctg ctgacactga aattacagat gctaaagtta ctttaggaac agaatcaaaa
    18241 acagccgctc cttatgtatt ctatgtagaa gatggtactt atgactatac agtagaaaaa
    18301 gaaggttatg ctactgtaac aggacaagtt gttgtaaacg acgcagctgt agaagaaaca
    18361 gttactttac aagctaacct agcagattgg tcagaaaaaa ttcttattgc acatccaagc
    18421 caactaggtt atgctactta tgctgatact agagttgaaa ctgctagcaa tgatgtaatt
    18481 ggagttgaat ataaacaaaa taatactgct ggtacagcag ctattattgt tccaacagct
    18541 aattgcacag gctttgtagt aatagctaac gatgcagcat atacaacaag tgacgattta
    18601 gaagctgctt atacagcagg taccgcagta actcaattag agctaggttt tacatatcat
    18661 gctccaaaag cttatgctga aaagaacttt atcgcaaaag ttggtaatga atatgtatgg
    18721 gtaaaatatg ttgatggaca tgtcgctcct agcgctactt cagatggata cagaggtaac
    18781 acacttgcat tccaatacaa aaaataatta tggtaatata taattaaaga ataaaaaaag
    18841 ccactcaaaa gtggcttttt ttatggcgct gtctcataag tgtcaaactg ctttacttca
    18901 acttcgttca gcaaccacgt tgctttcaat attcggactc agtcacgtac acttcggctt
    18961 tgctcagtgc aacgcttcat gtacgctcct tcgtcctcaa tttcagcgcc ttgcatttta
    19021 acacttctga aacaccttga aagtttatag ttttaaaagc tgtttttaga ttgcctcagc
    19081 aaggtgtttt attatgccaa aaataattta agtaaaataa atttggaaat tctatttatt
    19141 ttatttatct ttgtggcaca attgcggggt agagcagttg gtagctcgtc gggctcataa
    19201 cccggaggtc gcaggttcga gtcctgcccc cgctacaaaa acaaaagcac caattttggt
    19261 gcttttgttt tttaaacatt attttatgca gcaacaaggc ttgagcaaaa aatatggttt
    19321 tcataaaact aatttaataa taatatttat tagtttattt tgctttttgt ttgggcaatt
    19381 tgtttcagca ctaattttac atgccttcga tttaagcata gcagaaagtt tgttaaacca
    19441 tgcagaatta agttctaaag aaataaatgt tttaaaactt gctcaatttt tttctgcttt
    19501 actaagtttt gtgctaccaa gtgttttttg tgcttattta ctttatgaca attggaaatc
    19561 acaacttaga attacaaaag taaattataa atcttctttt tatttaatac cacttttttt
    19621 attgcttata atgccattta tgaatattat tattcagtgg aacgaaagca tagtgtttcc
    19681 agactcatta agagatttgg agttagcgtt aagagcttca gaagattcat ctcagttgtt
    19741 tataaagaca ttactacaag gcacaggagt ttttacttta ataataaata ttttgcttat
    19801 tggagctttg cctgcccttg gcgaagagtt gttttttaga ggtttgctac aatctcattt
    19861 gaatgattat tttaaaaatc cacatatagg aatttttatt tctgcatttt tattttcagc
    19921 tattcatttt cagttttatg ggttttttcc tcgtttgatt ttaggactta tttttggata
    19981 tttagtttat ttctcaggtt ctatttggac ggcagttttt gcacatttat ttaataatgc
    20041 tttagctgta attgttgtat attttcaaaa tttaggaaaa ataagtgaag aggttgaaag
    20101 ttttggaact tcatcttacg aaataattta ctcttttgta tgtttagcac ttgctgtctt
    20161 tgttgcacga agtttgataa ttaattttaa aaaacgaaat aaaattatta acttagtgga
    20221 ctaattatta tttataaaaa catggaaaaa ttactacaac aagcagcaga gacaataaag
    20281 aaggcaaaat atttaacggt ttttacaggt gcaggcattt caaaagaaag tggaatacct
    20341 gtgtttagag gaagtcaagg ggtttattct cactatgacc cttcggtttt agaaattact
    20401 aattatttta gaaatactga agaagcgtgg aaaggcatta aagctatttt ttatgatttt
    20461 attgggcagg cgaaaccaaa tttagcacat aaaattcttg ccgagtggga gagcaaagga
    20521 ttgttacgag cagtcatcac acaaaatatc gacaatttac attcagatgc tggaaataaa
    20581 acagttatag aatttcatgg tacaactaaa aattttgttt gtacacaatg ctcaagtaat
    20641 ttttctttaa atgaaatttc aataacagaa aaaccaccaa aatgtaagaa atgcaattct
    20701 ttattaaaac ctgattttgt attttttggc gagggaatac catcgcaagc agcaagtcaa
    20761 tcacaaatgt ttgctagcca gtcagatgtt catatagtaa ttggtacaac aggcgaagtt
    20821 tatccggctt catatattcc aatttatgct aaaaatgctg gtgctaaaat tattgaaata
    20881 aatccagacc cttcggcata tacaaataaa attactgata tttatcttcc gatgaaggcg
    20941 actgaggcaa tggagaagtt ggattcttta gtttttagtg tttagttttt agtgtttagt
    21001 ttttagtttt tggttttctg ctgttaactt tatttgagtt gtttttacta tgcttgttaa
    21061 catttttact aattccttgc aatttataat aaaaggcttg tagtcatatt ttaccatttg
    21121 gcttttatct aaaagttgta accaaaataa tgtttctctg gcttctttac ttgaaattga
    21181 cattttatat gcaaaatcag ctttacttat tccagctaac gcctcattga catttgctcc
    21241 tatacttgtt ccactacgta agagttgttt tgatatgaca aactcctttt ttcctcttgc
    21301 aatactttgt aaagttcaat aatctgtaat gcaaactcaa aacttttttc ttttactata
    21361 ctttctttca taatttaatt ttttagtgtt tagtttttag ttaaacttta aaaactttat
    21421 gaactttaaa aacttttaac tgatttattg cttttttccc attcccaagc tgattttact
    21481 gtatcttcga gagaggtttc ggctttccag tttagaactt tttcggcata agaggtatct
    21541 gcccacactt gttcaatatc tcccgcacgt ctgcttgtga tttcgtaatt tagtttttgt
    21601 cctgtgcttt tttcaaaggc tttaataatt tctaaaacag aatttccttt tcctgttcct
    21661 atattgaaaa attcatgatt tttggtattt ctcttattta tcaatctttc taccgcaaca
    21721 agatgtgctt tagcaagatc gcaaacattt atataatctc gaattgctgt tccatcagga
    21781 gtattataat cgtttccgaa gaccataagt ttttcgcgta aacctattgc agtttgagta
    21841 ataaatggca ataaattgtt tggaactcct attggtaatt cgccaattaa ggcagacgga
    21901 tgtgcaccta taggattaaa atagcgtaaa gaaatggctt ttaaattttc attggctttt
    21961 acagtatcgc taataatttc ttcgcttatt tgttttgtgt taccataagg cgacattgcc
    22021 tctttaatag gtgattgctc tgtaacgggt aaaatttctg gctgtccata aactgtgcaa
    22081 gaagaagaaa aaacaatgtt ttcgatattg gcttcgagca tatttgttaa aatatttact
    22141 aatccgccaa cattattttg ataatatttc aaaggttttt ctacagactc acccacagct
    22201 ttataagcag caaaatgcac aacagcttta gcatcacggt gttcttgcaa aatattgtta
    22261 atctcagttt cattgcaaac atcaacttta taaaaaatag gttttttatt agtaattttt
    22321 tctatccctt ctaaaattga aatatttgaa tttgataaat tatcaagaat aacaacttga
    22381 tagccagatt ctaaaaattc tacaacagta tgcgagccta tatagccagt accgcctgtg
    22441 ataatgattt tacacatttt tttaaattta agatgcaata attaaaaagt agtttttctt
    22501 tcctttttgc actaaaatat atttattatt aatcaaaaac tctgaattga taggagtttg
    22561 ttcatcatta aatttttctt tatttattga aagtccattg ccttgtattg tacgtctaag
    22621 ttcagatttt gaaggaaaaa tatcggtgtt tacacatagt aattcataaa tgtcaattcc
    22681 tttttcgata attgattttt caacattgaa caatggaaca ccatcgaaaa cgcttaaaaa
    22741 agttttttcg tcaagttttt tcaaaacatc gcttgtagca tttccaaata aaatcgttga
    22801 agcctcaagt gcatcttcat aatctttgcg agagtgaacc attacagtaa tttcttcggc
    22861 taattttttc tgcaatatcc ttaaatgcgg actttcttta tgttgtttaa tcagctcgtt
    22921 tatttcgttt ttgggtaata atgtgaaaat tttgatatat ttttctgctt cttcgtctgc
    22981 aacatttagc caaaattgat aaaaagcata agggcttgtt ctttcttggt ctagccacac
    23041 atttccagtt tcggttttgc caaattttcc accgtcagct ttggtaatca gtggacaagt
    23101 aagagcaaat gcttcgcctt gttctttgcg tctgattagt tctgtgcctg ttgtgatatt
    23161 tccccattgg tcgctaccgc ccatttgtaa tttgcagtta tattctttgt ataaatgtaa
    23221 aaagtcgtaa ccttgaacta attggtaaga aaattctgta aatgacattc cgcttttttc
    23281 ttcgccagaa atacgttttt ttacagaatc cttagccatc atataattta cagttatatg
    23341 tttgcctaca tcacgaataa attctaagaa tgaaaattta ctcatccagt cataattatt
    23401 tactaaaaca gcttgtgttt ttgctggtgt atcaaaatcc aaaaatttcg caagttgttt
    23461 ttttatagct tcttggttat gtcttagtgt tttttcgtca agtaaatttc tttcttgaga
    23521 ttttccactt ggatctccaa tcattccagt tgctccacca agtaaagcta taggtctatg
    23581 tccagctact tgcaaatgtt ttagcatcat cacgctaact agatgtccaa tatgcaaaga
    23641 atctgctgtt gggtcaattc caacataagc acttacaaat tctttttcaa ataattcttt
    23701 agttcctggc atcatatcat gtatcatgcc tcgccattcc aactcttcta caaaattcat
    23761 aatctatttt tgaattataa tttttttatt aataaataat tttccgtcaa aacttttagc
    23821 attcaaaaag tacatacctg attttaagtt ttgaactgag attgaaatat tttcattttc
    23881 ttcaaaaaaa tcttttcccc agcacaatct tccgtttata tcaaatatat taatttttcc
    23941 actaaatttt tcatcaagag caatttcaaa acaaccattg ttaggattag gaaaaatatt
    24001 gcttgttagt tgaattttat tattttctac ttgcaaatat ttttcccagt cataaattgc
    24061 aaggcaatat tcaccgcttt ttagagattc tactgtgaac ttttttaaaa attttgaatt
    24121 tgttgcattt tcaatttgcc aatctattgc acggtttggt ctatagagca atactaaaga
    24181 atctaaacta ttagtaataa actcgttgtc taaataaaga tttccactgc ctgtggcatt
    24241 agagtaagca aattcagcac tagctgaaaa attatttgga aaatttcctt ctacagtcca
    24301 aaatctattg tctgcaatga taagtccggg tatttcattt ttaaaattat ctggagcaac
    24361 ccaattatga gtaattctaa gcattgcagt gtcgttttcg tcagcagaat ttactattgc
    24421 tttaaaaaag gtgttgttat aattttttgt tcctgttgag ttaataaatt tggtttcgtc
    24481 tatacttgca tctgaaattt gttcgaaata gtcgcataaa ctaagcattg gattgaaagg
    24541 tagagtaaaa gtttgctcac caaattcacc atcaaaatca ataatttttg taatagtttc
    24601 aaaattttca ttcataaaac taatgggtac tttatttgaa tttgcaaata catttcgtcc
    24661 tcttaatttt tgtttcataa aaactgttac atcaaaatta ctgccatttg cacttacttt
    24721 agttgaatct atagagtagt gtacaaagcc tggtgtaaaa acccaatctt gaaaaaagtc
    24781 agtaaggtca atacctgtat gatttgtaat gaaatcgcgt aaatcgtatg aagattttga
    24841 tgtataggca ttttgtctaa gatattcttt aattgtagta aaaaatacag aatctccaag
    24901 atagcctctt agtgcatgtg caacacttgc tcctttttga tacacagtgc tagagtatgt
    24961 aatgtcttga cttatcggat ataaaggatg aaatcctcca tctttgtgat gataaacgcg
    25021 taaaacttgt tcgtgggcac ttcgtctaaa attgagagca ttttctttag attttacaaa
    25081 ttctctaaaa atggtttcgc aatacgtagc ccagccttca tttagccaca tgtcttcagc
    25141 tgatgagcaa gttactaaat taccaaacca catatgagca agttcgtggt aatataaatc
    25201 ttcgtatgtt aaatctcctg tgataaatcc tctgcccata gcaatatttg taacatgctc
    25261 catggcaccg ctattaaaag ctacggcaac ataaccaact ctgttccaag ggtaagctcc
    25321 aaacaaattt tcataaactg ccattacagt gtctatattt gcaaaagaat ttacagtttt
    25381 gctactatcg tttgggtgta tgtaaaaatc aataggaatt tctttttcaa ctccttgata
    25441 attgtgtgaa atcaatttgt atggacctac agcaaccgat actaaataag tagggacagg
    25501 tttttctaat ttccattccc aagttttagt gttatcttca gtatttgtaa taatattaat
    25561 aagttctccg ccacacacag cagtatgatt ttcaaggctt gtaattttac aagtaacttt
    25621 tgctttatct gtaaagctat ctttacaagg aaaccaaact ctgccatatg cgtgaggaat
    25681 atctttaaaa cctacaccca aattaaaagc ataatcaccc acaaaataaa aacctcccca
    25741 gccagatgga tcaaggatag gatttccatt ataataaact ctgatagata tttcatcttc
    25801 aagattaaaa gcagaggcaa aatttatatt taaaactgaa tcattgtaag tatagttaga
    25861 aatatcacaa gcattacaag ttatagagtc tacgtttaat tttaacaaat ctagacttat
    25921 gttatttata ttttcttcaa taggattaaa aataatatct gtatatccag aaatgaattt
    25981 cccgcttata tcagttatgt ctaagtttat gatatattct tgaatatcaa tattttctcc
    26041 gcttaaattt tgcgaaaagt tagaaatatt aattccaatt aaaaagctag ctataaaaaa
    26101 tatttttttc atttttaaaa aagagtttgt tgtcctgtat ttgggtttat tttatttaga
    26161 tgtctatatg ttttttcggt aacttctcgt cctcgaggtg ttcgttttat aaatccttcc
    26221 ataataagga aaggctcata aacttcttct acagttcctt cgtcttcggc tacagcagtt
    26281 gcaatggttt ttaagcctac aggtccacct tggaatttat ctactatagt tagtaaaatt
    26341 ttgttatcca tttcgtctaa tccatacttg tcgatattta gagcttcgag agcatgttta
    26401 gcaattttca tatcaattgt tccatctcca attacttggg caaagtctct aactcttctc
    26461 aatagggcgt tagcgattct aggtgttcct cggctacgtc ttgatatttc gccagcagca
    26521 tcgtcatcta ttggcatgtt taaaattcca gcagaacgaa taattatttt ttttagaact
    26581 tcagcggtat aatattttaa atgagaattt attccaaatc ttgcacgcaa aggacttgta
    26641 agtaatcctg aacgagttgt tgctccaatc aaagtaaaag gttctaaagg aatttgtaca
    26701 gaacgagccg caggtccttt gtcaattaaa atatcaattc tataatcttc cattgcagaa
    26761 tagagatatt cttctacaat tggatttagc ctatggattt catcaataaa taaaacatca
    26821 tttttatcta aactagtgag tagccctgca aggtcgccag gtttgtcgag tacaggtcct
    26881 gatgatattt taaatccaac tccgagttcg ttagcaataa tatgagcaag agtggttttt
    26941 cctaaaccgg gaggtccgtg caaaagcaca tggtctagag cttcttctct catttttgca
    27001 gcttgcacaa aaattttaag attagccaca atcttatctt gtccgctaaa atcttcaaat
    27061 tctagaggtc taagtatttt ttctatttct ttatcggcag ctgataaatt ttcggaatta
    27121 gctctgataa attcgtccac gtctatttat atttttttac aaaattacaa aatttgacga
    27181 ataaataaaa caataagcta caatattaat caattgtaat ttgttaatat tttcctgttt
    27241 tcagcgttgc aacacccatg ccgaaaaatt acgccttgcc cgcaagggca cgtcagttaa
    27301 aagttataag agtttttagt gtttagttta ttgctcgaat agatttaact ttacgaactt
    27361 taaaaacttt attaactttt aattgttttt attgtgttgc ccttgcgggc aaggcttttg
    27421 atggtctaaa taccgcaggc tgcgcctacg gttaatcaca tgttgtccta cggacaaaac
    27481 ccctgatagt cttatattgt ccttacggac aattatatat gtatgtgctg tttaaaatgt
    27541 tgtaacaccc aaacacgata aattatgctt gattatcaaa gacttagaaa gtgttgcaaa
    27601 aaaagtacgg aaaacaggaa ttgtaatttg ttaatatttt tgtgtaattt tgtttcaaat
    27661 cgcggtaaaa gcaaaaatgc aatttaattg gctaagattt ttaaaatctt tattattttt
    27721 gtaaaaaata taaaaagtga ttttatcttt tatacatatc agatttttag acattattga
    27781 tattttatta gttgcctttt tgctttatca gctttactca ttgataaaag gaactgctgc
    27841 aataaaaatc tttattggta ttgcattgat atatttggta tggctttttg taaaagctat
    27901 gcgaatggaa ttgataagtt ctattttagg acaagtaata ggagtggggc tgattgcttt
    27961 gattgtagtt tttcagcaag aaattcggca atttttatta cttattggga ataaatacat
    28021 aaaacgtagc aaattaaata tacttagaga ttcgcgttta caatctggat atagcactag
    28081 ctctatagaa tatattgctc aggcagcaaa tgaaatgagt atagctaaaa ctggagcttt
    28141 gattgtaatt tcgcgtgagg cttctttaaa aagttttatt gagcaaggcg aagttttaaa
    28201 tgcagatatt agtgttcctc ttttaaagtc tatatttttc aaagatgctc ctttgcatga
    28261 tggagcggtt ttaattgaaa ataataaaat ttatgctgct aaatgtattt taccagtttc
    28321 agcaaataca gaattaccta aagactttgg tttgcgacat cgctctgcat tgggaatggc
    28381 tgaggtttca gatactttaa ctgttgtggt ttcagaacaa acaggcaaaa tttctgtagc
    28441 aaaagagggt aagatttata gactaacaac aaatgataga ttaagcaaaa taattgcaaa
    28501 ctttttacga aagaatgatg attaagtttt agattctttg gtaagctgtt aatgtgtttg
    28561 aagaagaatt ttaatttaaa agtttgttaa taaaaatgtg ttatataatt attaatattg
    28621 gtttatatga tgtatatttg tcagtatgaa agaaattaat gatgtaatac aaacaaaatt
    28681 tgctgtttct aaaaaaatcg atgaaagttg ggattttagg aaagcaaata ctaaagaatt
    28741 gacacattgt ttccattctt atcctgcaat gatgattcca caagtggcga ggagaattat
    28801 aaaaaactat ggaaaagaag gagatgtatt atttgatcca tattgcggta caggaacatc
    28861 attagttgaa gcaaatatta aaaatatatc agcaattgga acagacctta atccattagc
    28921 acgcttaatt gcaactgcca aaacgacaaa aataaattca gatttattaa actcggaaat
    28981 taataatttt ataaactatt cgtttaaatc gaattttcaa aacaaaaaat catattccaa
    29041 aataattcct gatgttacaa atattgatta ttggtttagt aagggtacgc aagaaaaatt
    29101 aggaactatc tttggttata ttgaaaattt aggtaacgat tctataaaaa acttttttaa
    29161 agttgctttt agtgaaactg taagagatgt ttcgtttgtt aaatctggag agtttaaaat
    29221 ggttagaacc aaaaactttt gtcatgaaga agataaagac gtttttggaa taatgtcttc
    29281 aaaacttgaa agaaacaaat taggtttaga agactttagt gctgtgtgtg caaatggcac
    29341 aaagtcagca gtttatgggt tcgacacagt agttggtatt cccaatttta tagattcaaa
    29401 tagtgttgat attgttgtaa catctcctcc atatggcgat tctcgcacta ctgttgcata
    29461 tgggcagtat tcaaggcttg ctaatgaatg gcttggttat gagacagcta atcaggtaga
    29521 taatatttta atgggaggca gaaagcgaaa atatgatcac aaatttgata gtgagattct
    29581 taacgatgcc attttaaaaa ttcaatcaaa agatattcaa agagcaagag atgttatttc
    29641 tttttatgaa gactatgaaa gttcaataaa aaatgtttca aaacttataa aaaaaggagg
    29701 ttttgcttgt tatgttgttg gcaatagaac agtcaaaggc gttactattc ctaccgatga
    29761 gcttactgct gaaatttttg aaagttgtgg ttttgttcat ttagaaacaa tagttagaaa
    29821 tattcctagt aaaagaatgc catatagaaa tagtccttca aatattaaag ggaaaacatc
    29881 ttcaactatg aaacatgaat atattgttgt ttgtaaaaaa ctttaaatat gcgattgcca
    29941 gatttgataa aagctcttga aagcttgaag gaagaaggat ttataccaac taaacgtaaa
    30001 tgtccaacag gtattggaca tttacttgaa aatatgctag gattaacaga aactaatatt
    30061 gccattcccg atattggagg tagagttgag ttaaaagcaa ctagaaaaaa tgccaattct
    30121 ttaattactc tttttacttt taatcgtagt gtgtggaaat tttcgcagac agaaattatt
    30181 aaaaaatacg gctatataga cgacaaaggc agacaggctc tttacacaac agtaaataat
    30241 aaaactccaa ccaatcaagg atttatattg gcttcagaac cacaaaaaaa cttaatagtg
    30301 ttgagaaata ttgaagagga aggtaatatt gcagaatgga gtacttatgt tttagctggc
    30361 aaatttatga ctaaacttga tcgtttattg ttagtttttg cagatagtaa aattgaaaat
    30421 aatgttgaac attttcattt taatgaagcg tatttgttag aaaatccaac tcctgaaaaa
    30481 tttttagatg catttgaaac ttgcaagctt atgattgatt taagaatgta tttaaaagat
    30541 tctggtggtg ttagaaatca cggaactgga tttcgtatat cagaaaaaaa tttgacagaa
    30601 ttatatgaga aaaagagaag attgatttaa ttgtatggca taatgtgtta attacatttt
    30661 ttttcttata tttgtaaaaa gctattttag agatgaataa gaaaagtgta aatacaattt
    30721 taattttctg tttaattttt tgttttttct ccttattttt attttcacag aacacaaaaa
    30781 atcctacaat tatgaaccgt aaagctgctg ttgctggtca attttatgcc ggcactaaag
    30841 aaagtttaaa ccaagatttg aaaactcttt tttcaaaagc tgaagaaaga aaatatgatg
    30901 caagagcaat aatttcgcca catgctggct acgtttttag tggagaagtg gcggcttcgg
    30961 ctataaatca aattaatccg aacaaaaaat atcaaaatat cttcattatt ggttcaagtc
    31021 atacaaaata ttttcctggt gtatctattt ataacatagg taattatgaa actccgcttg
    31081 gcgaagtaaa agttaatatt gacgtttgca atgagttgat aaaaaataat aattttatct
    31141 cttttgttcc cgatgctcat agaacagaac atagtataga ggttcaattg ccgtttttgc
    31201 aatatcatct aaaaaatgat tttcaaattg tgcctatgat acttggtttt gatggagaac
    31261 aaaatgcaaa aaaaattgca gaagcactaa aaccatattg gaaggaagaa aatattttta
    31321 ttgtttcaac agatttttct cattatccaa gctataatga tgctgtaaat attgacttag
    31381 caactgctaa tgctatagta tctggcaaac ctgaagagtt gattaaagtt gtaaattctg
    31441 aaaccgggat aaaaaatctt tctacaaatt gttgtgcatg gaattcggtt ttgagtttaa
    31501 tgtatttaac agatcaaaaa tattcctaca ataaaattca atacaaaaat tctggcgatt
    31561 cgccttatgg cagtgatgat agggtagtgg gctattgggc aatttctatt actgataaaa
    31621 acgattctag tttctcatta agtgatgaag aaaaaatgga gcttttaaaa cttgctcgta
    31681 tgagtgctga ggcaaaaatt aaagaaaata aaaacttaga tgtttctaat attgttgtgt
    31741 cagagaattt gaatgagcaa tgtggtgctt ttgtaacttt taataaaaat tcaaatttac
    31801 gtggctgtat aggtaggttt gaacccaata ttccgcttta taaggtcgtg gtagaaatgg
    31861 cagtagccgc agcaacacaa gattatcgtt tttcgccaat ttcagcacaa gaattagacg
    31921 aagttgaaat agaaatttct gttttaacgc catttaaaaa aataaaatct attgatgaaa
    31981 tcgaacttgg caaacatggt atttatattg tgaaaggtgg tcgtgcagga acatttttac
    32041 ctcaagttgc agaatctact ggctggagtt tagacgaatt tttgggatat tgcgctcgcg
    32101 ataaagcagg aataggttat gatggctgga aaaatgctga tatttttact tatgaagcaa
    32161 tagtgttttc tgaaagtgat tttaaaaaaa aagataaagc aaaatattac gaaaaattag
    32221 aaggcggaag agttctctgc aaattatgtc cacacgaatg caagctaagc gaaggtaaac
    32281 ttgggttttg caaagcaaga aaaaatgtaa atggtgaatt aattagccta agttatggaa
    32341 atttagttgc aattcatatt gacccaatag agaaaaagcc tttatatcac tttatgccgg
    32401 gttctacaag tttttcaatt ggaacagcag gttgtaattt gagttgtaaa aattgtcaaa
    32461 attccgaaat ttcgcaaagt tctccactgc aattagagcg tattaaagct acaccagaac
    32521 aaattgtaga agctgcaaag aaaaacaact cagcatcaat atcttacact tatacagaac
    32581 caacagtttt ttatgaattt gttttagaaa ctgcaaaact tgcgaaaaag aacggtatca
    32641 aaaatattat aatttcaaat ggatttatta atcctgaacc tttaaaggaa ttaattccat
    32701 atttagatgc tgcaaacatt gatttaaagg cttttgacga ttccatttat agggaaatta
    32761 caagtgcaag attagagcca attttgaata atttaaaaat tttgcgtgat agcggtgttt
    32821 ggttagaaat aacaaattta attattccag gtcatacaga tgatatgcaa atgatttcag
    32881 aaatgtgtga atggttagtt gaaaatggtt ttgcagaaac tccactacat tttagtcgtt
    32941 tttttccagc aaataagatg atgcatgttc cgccaacttc ggttgaaaca gttgaaaaag
    33001 ctgctaaaat tgcaaagtca aaaggaataa aatacgtata tctcggaaat gttcatcaag
    33061 aagaaggaaa tacaatttgt cctaagtgcg gaaaaacttt gattaaacga aaaacttata
    33121 gaacagaaat tgatggtttc tctggcaaat gtggaagttg cggagaagta attaatggag
    33181 tttggtagtt tttgtctgaa acaaaaatat aaataataaa aatttcagtt tttctttttc
    33241 gcttttagtt taaactctaa ctttgcaaaa tgaaaggaca aactagttcc aaaatagaat
    33301 taagaaaaat gttgctggct atggtaattc ctgctagctt tatgttttta gcatggatgg
    33361 tttttgcttt agaaaaatct ttgcatataa gtttttcaga gtttggtttg cgtcccagag
    33421 attttaagca atggtatgga attttaacta tgcctttttt acatggcagt cttgagcata
    33481 tactagcaaa cacagggagt tttcttattt taggttcaat gcttttttat tttcataacg
    33541 atagagcaat actacttttt attttatctt atttttttag cggtatttta acatggctaa
    33601 taggtcgtga aagtgttcat attggagctt cggcaatgat atatgctttt gcaggttatt
    33661 tgtttactgc cggtttaatt ggaagaaata ttaggcgaat ggctgttaca ttaattatcg
    33721 tgtttttgta tggaagcatg gtttggggaa tgttgccaat aaatgaaaga attagttggg
    33781 aaggtcattt gtcaggtttt ataacaggat tggttttagc attgattttc acaccgccag
    33841 ctccaccaac tttttcagaa gaagatgatg agcttgataa tgaggaagat agatattggg
    33901 aaatagacga aaataaggat aaagacaaat atttaaacta aaaatgcaga ataaacaaaa
    33961 tacaaaactt ccttggaatg attattcatc atatataaaa cgcatttttg gaattagagt
    34021 gcaaaaaatt tctattgatg caggttttac atgcccaaat cgtgatggaa aaatctcaag
    34081 aggagcttgt ttttattgca ctaacgagag tttttctcct ttttattgca caccaaaatt
    34141 atcggtaaca gagcaattac aaaaaggaat agagtttttt agtaaaaaat ataaaactca
    34201 aaaatatttg gcttattttc aatcatacac aaatacttat ggaaaagttg aagatttaaa
    34261 aaagttgtat ttagaagctc ttgctgttga gggagttatt ggcttagtaa ttgctacacg
    34321 accagattgt atagatgaag aaattatgaa aatgctgaaa gaaatttcaa aagataaata
    34381 tttgagcata gaatatggag cggaatctac aaaaaatgaa actttaaatt ttataaatag
    34441 aggacatagc tgggaacaaa caataaaagc tgtagaattg gcaaataatt ttcaaataaa
    34501 ttgtggttta cattttatta ttggacttcc gggagaagac aaaacagatt ttttaaacca
    34561 tgctaaaaaa atttcaaaat taaatattca aactttaaaa attcatcaaa tgcaagtctt
    34621 gaaaaaaact aaacttgcag atgtttataa agaaagtcct gaactctttt ataatttaaa
    34681 tttggaaaat tatcttgatt tgatggtaga gtttttaggc tatttgaatc caaatgttgt
    34741 tgtagaaagg tttacaagcg aatcgccaaa agaaatgata atctatccaa attggaaagg
    34801 aaaaaagaat tttgaaataa gccatatgct tgagaaaaaa atgaaagaga gaaaaatcac
    34861 ccaaggaatg ttttttgaag ctaattctat atagactttg gtgttgtttg tcagtttaga
    34921 ttgagtgtaa gtataaaatc acattgattt gctaaaatta attttgcaaa tgaacatatg
    34981 ttcattatat ttgtaatgca caaatgttca aaaatgagat atagaaaaag aagacataga
    35041 aaacttttga gtccgcctgt tattaaaggt tttatacctt acggaaagga tattaataca
    35101 gaaaatcagg acttagttag tatgttgtgt gaagaatacg aggcaataaa actttgtgat
    35161 tatgatatgc ttactcatct tgaagcatct gctttaatgg gtgtttctcg tccaactttt
    35221 actcgtattt attcttcggc acgtcaaaaa atagctttgg caattagcga agggcgacat
    35281 ttgataattg aaggcggaaa agtttattac gatagccact ggtttcattg taaggactgc
    35341 gatgcttttt ttactaatcc taatgatatt gttgacatta cgtgtgcttt atgtgcttct
    35401 tctaatgtat caaaagttca agatgaggaa tctgtaagtt taaaatgaaa atatagtaat
    35461 aattaaaaaa ttgaaataat atgaaaattg caatcacatc aactggaact acattaggtt
    35521 ccacaataga cccgagattt gggcgtgctg cttattttgt aatttatgac attgagaata
    35581 aatcttttga aatttgtcca aacccaaaca aggaagctac tgaaggagca ggtcctgcat
    35641 ctgttcaatt tgttgcagaa aaaggagtag agaaaattgt atcaggcgaa tttggagtaa
    35701 aagtcaaaga tttattagac tctttaaaaa tacaaatgat tgctataaaa gatcatagca
    35761 agacagttca agaaattata gatatgttaa atcaaaataa ataatattat gccaaattta
    35821 gatcaaacag gtcctagagg acaaggtgcc ggaacaggac gaagagctgg aaaatgtttt
    35881 caaggctcgg attcagaaaa tgtaaatgaa caaaacttag gtcagggaag aggagcaggt
    35941 ctcggtagaa gaggaggacg ctgcagaggt gctggacgtg gcttaggtag aggtttaggg
    36001 agaaattaat aataatgttt tagcaggtga acttcgcctg ctgttttaat tttaataaat
    36061 tatgaaaaaa attttagctt ttccattagc aaatggagtt ttatgtagtc attttggaca
    36121 ttgcgaaaaa tttgcaattg ttgaagtaga agacaaaaat attgtaaata ttaaagaatt
    36181 aactccgcca gaacatgtgc caggcttgta tccaaaatgg gttgctagct ttggtgttac
    36241 cgatgttatt gccggaggca tgggacaaag agccatagat ttgtttaaag aacaaggaat
    36301 taatgctttt gttggagcac caataaaaac tgccgaagaa ttagcaaaag aatttattga
    36361 tggtagcttg gagcttgggg ctaattattg cgatcattaa tgttactatt tagtttttta
    36421 tacaaataaa tttacttatc aaagctaagt taaagacggt gtttcataag tatcaaactg
    36481 ctttacttca acttcgttca gcaaccacgt tgctttcaat attcggactc agtcacgtac
    36541 acttcggctt tgctcagtgc aacgcttctg gtacgctcct tcgtcctcaa tttcagcgcc
    36601 ttgcatttta acacttctga aacaccttga aatagtgtcg gtttaacact tgttttgcga
    36661 caatctcttt aatttacagg cttttaattg cttaatttca ttattttaaa gaaagtttat
    36721 gcaggagaat aagaaaaatt caattagtat agctgtggct agtggcaaag gtggcacagg
    36781 aaaaactatg ttgtctttat cgctttttaa ttctattaaa aacaaatatg atgatgtagt
    36841 tttgctcgat gctgatgcag aagaaccaaa cgatttactg ttttttgatg ccgaagaaat
    36901 tagctctaca gatgttactc aaaaaatacc agttatagat gttaacaaat gtaagttttg
    36961 tggcaaatgt cgtgattggt gtaattataa tgctattttt cttcttgcta gtacaaaatt
    37021 tattaaactt accgaagaac tttgtcatgg ttgtggtgct tgctctgtag cttgtgagtt
    37081 tggtgctata acagaaaaag atgcaagttt aggaagtgtg aaaaatttta tttgtgatgg
    37141 aaagaaaaac ttgtatgaag ctatgatgca tcaaggggtt atgacaccag taaatgttat
    37201 taaagtagct cataaaatta caaataagca taaaattaga ataattgatt cgccaccagg
    37261 cacatcttgt ccatttattc atgcagttgc agaagttgat tatattattt tgattacaga
    37321 accaacacct tttggtttat cagatttaaa acagtcgatt tctacattag aaagtatgaa
    37381 taaaaaatat ggagttgttg tgaataaagc aggcttaggc aatagagcga tttatgatta
    37441 tttgtctagc gaaggcatag aattactttt ggaaattcct tttgataagg atattgcaag
    37501 aaaatattct gaaggaaaaa ttatatctga ttttaagcca gaattagcaa aagatttaga
    37561 aaaaatgttt gattatttat tgaataagta tggaaatagc agtaattagt ggaaaaggtg
    37621 gaacaggaaa aagcagtatt acggcagcta taactaatgc tctaggcaat gttgttttag
    37681 ctgattgtga tgttgataca gcgaatttac atttattatt taatcctgaa atagaagagg
    37741 aagaaagttt tgttggagca gaaactgctg taattgacta tggtatttgt attaattgtg
    37801 gcgaatgtat gaattactgt cgttttgatg caattagtaa ggataaaaat gaaaatattg
    37861 taatttcaga tattttttgc gatggctgtg gactttgtga aaaaatttgt cctgaaaaag
    37921 caatttcgat gttggcttca acagatagca aacttttatc aggtaatttt agatttggta
    37981 aaatggtttt aggacatctt gctccgggtg aagaaaattc gggaaaactt gtaaatttag
    38041 ttagagatag agcgagagaa atttcaaaaa ccaacaatat agagcatatt atcattgacg
    38101 gtccgccagg aataggttgt cctgttattt caacagttac aggtgtggat tattgcatta
    38161 ttgtaacaga gccatcgctc tcaggcttgc atgatttaaa acgcacaata gaattagttg
    38221 aaagttttaa actgaacttt ggaattttga taaataaggc agatttaaac gataaaataa
    38281 atgtaaaaat tacagagttt tgtaatcaaa ataattacaa tttattaggg caaattaatt
    38341 ttgacactaa ttttgttgaa gccatgttaa ataccaaaac aattggtgaa atgtttccag
    38401 aaagtgaatc gtatggtgtt gtaaagcaaa tggttaaaaa agttttactt agtcttagcg
    38461 aaagttaata ttatatttat atttttgcag aagactaaag tttggtatta tggaaattag
    38521 taaaactcaa attctttcgt atttagaaaa atttaaaaca aaaagagttt taattattgg
    38581 tgatgtaatg attgattctt acatgtgggg agttgttaat cgtatttctc ccgaagctcc
    38641 aattcctatt gtgtcggttg tgaatagaga atatagaatg ggaggagcag cgaatgttgc
    38701 ttttaatatt aaatctttag gtgctgaacc aattttatgt gctgttgtag gacaagatga
    38761 aaaaggaaag atttttagag acttgttgaa acaaaattct atgtctgacg aaggtttact
    38821 ttctttgcca gatagaaaaa caacaataaa aacaagaatt atttgtggtt cgcaacatat
    38881 gttaagggtt gatgatgagg ttgataccga gattgatgag aaaagttcgg aaattttggt
    38941 agaaaaagtt gaaaaaatta ttgcaaaaca aaaaattgac ttgataattt ttgaagatta
    39001 tgataaaggg gttttaaatc ctaaatttat ttcaaaagtt attaaaattg ctaagaaaaa
    39061 ctctattttc acagctgttg accctaaaaa acgaaatttc aattcatata atggagttga
    39121 tttgtttaag ccaaatttta aagaattttg cgaaggtaca aactcagaaa tttcaaaaac
    39181 tgatttaaaa gaaattgcaa aatctgcaca aaaatttctt gaatcttctg cgataagcac
    39241 tcttttatta acactttctg agcatggagt ttttatttct gatataaaaa ataatttcca
    39301 ttttcctgcc gaaagacgat tgattgctga tgtctctggg gcaggggaca ccgcaataag
    39361 tgttgcagga ttattgctta gtgcaggagc ttcactatct gaaattgcta gtatttcaaa
    39421 tattgcagga ggtttagttt gtgaaaaatc tggcgttgtt cctattgagc ctgaggaatt
    39481 gcagaatgaa tttaggtaaa ttatgattat ggcggtgtct cataagtgtc aaactgctga
    39541 cctgagttca taaagttaaa agttcataaa gttccttaaa tccgcaacta aaaataatta
    39601 ttgcaatttt atgtaaaatt at
//
LOCUS       DAOPNX010000014        39436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_1354_length_39436_cov_7.746578, whole genome shotgun sequence.
ACCESSION   DAOPNX010000014 DAOPNX010000000
VERSION     DAOPNX010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 39436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39436
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1354_length_39436_cov_7.746578"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1237)
                     /locus_tag="PKN53_02735"
     CDS             complement(176..1237)
                     /locus_tag="PKN53_02735"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOD87900.1"
                     /translation="MLKINKIKSPTFAVNTDIVKKNIQKIQNKLGETTIFRPHFKTHD
                     NKQTANIFRELGINKITVSSIEMAIYFRRLGFKDINISFPINFREQKALQKLSKNTNL
                     HLLVSNSFSAKQLSSLNDIKANVWIEIDTGYKRSGISHNNLKEIQDCIKLITQASHLN
                     FSGFLNHNGETYLLNKKEEILQSTLGSIAKMNFLKSEFSNYSPKISIGDTPGCSLLDN
                     FDGIDEIRPGNFVYYDLMMLEKQVCTKEQIATTLFCPIVDINNDRKEIIIHGGAIHFS
                     KEFIEFNNKKIFGRAIKHYSTDISEINEDIVSLSQEHGIVKINNSNFENYKPGNLIEI
                     IPVHSCLAANLMKGKTRHY"
     gene            1432..3126
                     /locus_tag="PKN53_02740"
     CDS             1432..3126
                     /locus_tag="PKN53_02740"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD87901.1"
                     /translation="MQNKRIVVIGGSAAGPKVASRARRLDENAEITMFQKDADLSMAS
                     CGYPYYVGGFFDDRSQLLCSPAGVVRDSNFFLNAKNIVAKVNTEVTKIDRKAKTIDYR
                     NLITGEKSSMEYDKLVIATGATPRRPPIPGIELEGISTLSSMPDADYLRKVSDEGKIK
                     KAVVIGGGLIGIETVEALHLAGIEVTLVELLPQLLTFLDRHLARLVQQHVQSKANVIL
                     ENGVSAFIGENGKLTAVKLNNGTELPCDLAVLAIGVTPNSKLAKDAGLEISERGGVVV
                     DEYMQTSDPDIYALGDCVEIPNLITKNKVHAPFGDLANLQGRVVGENLIKGNVAKFEG
                     TIQTGVCKVFDYAAGTTGLNEQNARKNGFNDIETVINVSLDKPGFMNGNLLITKLVVN
                     KADRKILGAQVIGPGDVSKQLAIWAIAIKGKLTVDDMINADLPYAPPFSLAIDHSIAT
                     AHIMQNKLRGLFKGISSEDVEEKVKNKENVVYLDVRNPNEYEQMRIGIGERLIPLGQI
                     RKRISELPEDKNTEIIVWCKISLRGYEAALVLQANGYTNVKLMEGGVMAWAGKREK"
     gene            complement(3288..4115)
                     /locus_tag="PKN53_02745"
     CDS             complement(3288..4115)
                     /locus_tag="PKN53_02745"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOD87902.1"
                     /translation="MIGHIEAINIRRSIRNYSKEKLDSNLIEKINELLSTPKIGPFDN
                     RPRFVLIEKPKAREQEKVRLGTYGFIDNAAYFIGGCIEKSAFSEVDYGYSLQRIIIEL
                     TRLGLGTCWLGGSFRRKDYRKLLNTQKNEIIPCITPVGYKSTKKSRRERLGLVFSDGS
                     LRKDFNTFFFENNFETPIIFNPEDNYHKALEAVRKAPSAMNKQPWRVLKIEDKYHFYL
                     KRDSIVGTTKASDLQKVDMGIALANFKLALEELNVQGKWHIADPNIGNLEYIITWIS"
     gene            complement(4131..5777)
                     /locus_tag="PKN53_02750"
     CDS             complement(4131..5777)
                     /locus_tag="PKN53_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOD87903.1"
                     /translation="MKKIILLALSLFAIYSSSISQIESVIPSDKPKLVVGIVVEQMRQ
                     DYLERFWNNLGDKGFKKLAINGSFCQNANYEYSLTQAAPGYATIVTGCEPSEHGIVSD
                     YWFNALTGNKEHCIETKSYKTIGGNPSNFSYSPKNILTTTFSDEAKLFGQGKSKTISV
                     SLDPIGAIISGGYTADAAYWFDTKTGNWVSSTYYMNELPAWVKEQNDKKMPDDYLQRE
                     WSPLLETEKYNTAQADSSIYEYGIDMVYKTFPYKYEEISKSIKDYELLNLIPEGQTHL
                     TDMAIAALYNEELGKDKFTDFLFINYSVSENIGKLFGPQSMEVEDLFLRLDRDLGHLI
                     SVIEDVVGKNNVLIYLTSNHGVSENPEYLADNKMPTGVFKQHYILTLLRSYLKAIYGE
                     GDWVLDYNNNQIYLNKTLIEDSQISLNEFQEKVISFIINSAGISNAISSHQFQNIIFT
                     QGMPKKMQNSFNQKRSGDIMISLKAGWIEDIPFVCNHNSGYKYDTNVPLIWYGWKVKK
                     QNIFNNVNITSIAPTISKILNTPAPPASTGEVLNSVFNNK"
     gene            complement(5871..7088)
                     /locus_tag="PKN53_02755"
     CDS             complement(5871..7088)
                     /locus_tag="PKN53_02755"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOD87904.1"
                     /translation="MLDINKIRSDFPILNEKVYNKNLIYFDNGATSQKPKQVIDTISE
                     YYSKYNANIHRGVHFLSNFATDANENARKIIQNFLNAKSEKEIIFTKGTTDSINLVAF
                     SFGETFINEDDEIIVSEMEHHSNIVPWQLLCERKKAKLKVLKFNEIGELNIKELDNLI
                     TNKTKLISVAHVSNSLGTINPIKTIIEIAHKNNIKILIDGAQAVQHLKIDVQELDCDF
                     YVFSSHKAYGPTGVGVLYGKEELLNQMCPYQGGGEMIANVSFEKTTYNELPFKFEAGT
                     PNYIDNIAFGKAIEYISEIGLKNIEDYENQLLKYCTKQLKTIENLKIIGEAEHKSGLI
                     SFIVKNIHYNDIGTLLDRAGIAVRTGTHCAEPVMKKFGISGTVRVSFAFYNTFEEIDY
                     FITNLKKAISMLQ"
     gene            complement(7185..9038)
                     /locus_tag="PKN53_02760"
     CDS             complement(7185..9038)
                     /locus_tag="PKN53_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD87905.1"
                     /translation="MKRHLLILISLLLGINYINAQVLYSDDFESYTVGNGIAEESSTW
                     WNTWNGTPGSAEDPTVSDAQAYSGTKSVKVINDNDGVIDFGGLTTGRYRVEFYLYVPA
                     GRQAYWNIMQQFNPSGSGLVWGLQVFLKNGTMTIDGNGPSAATYSYTPGEWMKIQHFI
                     DLNSDWVDFYVNGELVHAYQWSKGATGTGVNKLGAFDFYGWKEGGTCEYYMDDFLIEE
                     VETPYPPTNFSYTIENGNDVVLTWEAPTEGTPVNYSIARNGTIISTTTETTVTDLNVY
                     PGTNKYSLLAFYGESNGYSAPLNLNVTIEGGVERNFVMYEMFTGTWCGYCPTPAQAIA
                     QIQATDKKVAVIKFHGGDSFETSATATRESYYGSIIEPIEGYPASVINGTQYLSGSAP
                     DVASQKEYFEYYYDEFIDVPAIYTLDLATEIISEKPYKCKLTVNAEETFGYYDDEIRL
                     MIALTETNIPYSWGGLSTVDNAVRAMYPDANGTVIFDSGTTYSGEFEITPASNYVINK
                     CEIVAFIQRRSTGEIQQVNKIALPEPSNIENNITKSLSIYPNPASDIVKVNSEENINY
                     IEILNISGQIVSFENVNTNNAEINISNLSSGVYFIKVYTANDVTTQKLIVE"
     gene            complement(9248..11026)
                     /locus_tag="PKN53_02765"
     CDS             complement(9248..11026)
                     /locus_tag="PKN53_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOD87906.1"
                     /translation="MKTYCNKLAKRLLIWRVRKISDKNFMLILAVIVGIAVGLAAVLI
                     KNSVHFVREVAIFLNNKYSFSYIYIFFPAIGIGLTLLFVNIFIKKKLGHGIPMVLYSI
                     SKERGKIEKHNMFSRIVASSLTVGFGGSVGLEGPIVGTGSAIGSNIGQFFRLNYRQLI
                     LLIGCGCTGAIAAIFKAPIAAIVFTLEVIMIDLTMGSLMPLLVASISAALTSYLFLGQ
                     NYVYHVANIDPFFLKDVPLYILLGIIAGLLSTYFSKLYLWQGKQYAKLKTKRARFIVG
                     VTILGVLIFLFPSLYGEGYEVINLALHGDISYIFDGVFYSQWQSFLGICIIIILILLT
                     KAFATGATFGAGGTGGIFAPSLFLGANLGFLFAHVANSMKLNISHGNFALVGMAGLIA
                     GVIHAPLTAIFLIAEITGGYELFIPLMIVSTISFFVVRIFMPKSVYAILLAERGELLT
                     HNADSNMLTLMRTESLIEKNFLTINENAKLKDLTKVISRSSRAVYVVVDDDNYFKGLV
                     WLDHVKHLMFKTELYDSVYVRDLMDIPKYTVNTNMDVKDIAEIFENSNYFNLPVVDEG
                     KYVGFVSRAKVFTTYREMLKKFSHDE"
     gene            11132..11887
                     /locus_tag="PKN53_02770"
     CDS             11132..11887
                     /locus_tag="PKN53_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOD87907.1"
                     /translation="MKKYKIGIALSGGGVRGFAHLGVLQALNERGIFPEIVSGTSAGS
                     IVGAFYCAGYSPHDALEIMKTKKFSDFTKIHIPKDGFFGLENLKLILENNIKQLNLED
                     LNIPLYVCVSNLNTGKVEYVNKGPLHDMILASSAIPVVFSPVKIGKTYYVDGGLIDNL
                     PVKPLVRLCDDIITVNVFPNDENQKFDSLLQVAIRSFQMNMNNNIKYIKSKSTVFLEP
                     AALSKYHILKTKYQEEIFEIGYKYAKKAKFELS"
     gene            complement(11884..12345)
                     /locus_tag="PKN53_02775"
     CDS             complement(11884..12345)
                     /locus_tag="PKN53_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD87908.1"
                     /translation="MLEINYRLANENDFAAILEMIKELALFENAPDKVLNTTEIMKQE
                     QNLFQCFVAETKEKEVVGMALYFFAYFTWVGKSLYLDDLYVKPQYRGKGIGTELLKLI
                     FKTAKEENCRRLRWQVLNWNTPAIKMYEKVGATIDAGWSNCDFEYHQILKF"
     gene            12548..13174
                     /locus_tag="PKN53_02780"
     CDS             12548..13174
                     /locus_tag="PKN53_02780"
                     /inference="COORDINATES: protein motif:HMM:NF004132.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOD87909.1"
                     /translation="MKHFKIQGTLRTELGKKATKAIRKGENIPCVIYGGEKNINFYTP
                     FSAVRKFVYTPEVMLADINVDGKDYMCMVKEIQFHPVTDSILHIDFYQITPTKPIIIQ
                     IPVKTVGNSIGVRSGGRLKQNYRKISVKGLMDDIPDYFEVDITPLNIGQSIRVRDLKS
                     DKLEFIDPKANVVVSVVVTRTVSSTGEEEGEEGAEGEGGEEEKTETAE"
     gene            13194..13757
                     /gene="pth"
                     /locus_tag="PKN53_02785"
     CDS             13194..13757
                     /gene="pth"
                     /locus_tag="PKN53_02785"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOD87910.1"
                     /translation="MKYLIIGLGNPGDEYKNTRHNIGYTVLDTLAKKSSTSFSDKRYG
                     FVADIKHRGKTMILVKPTTFMNLSGKAVDYYIKKEKVPVEKTLIVVDDIALPFGSIRI
                     RNKGGSAGHNGLEHIEYTLGTNVYNRLRFGIGNDFNKGGQVDYVLGEWTEEEKLLLPA
                     RIELAIEAILSFPILGIERTMNFYNNK"
     gene            13757..14563
                     /gene="asd"
                     /locus_tag="PKN53_02790"
     CDS             13757..14563
                     /gene="asd"
                     /locus_tag="PKN53_02790"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HOD87911.1"
                     /translation="MSNSKNNKQNYRNLSSRFIGKFSNIKKPRCLVNAVIKLYKRYYK
                     IDLNGFVVPENGFESFNSFFTRKLKVGVRPISNGIVSPIDGSLFDFGKISSENKIYVK
                     HKHYFIDELIISDYDGLKSYAVFYLSPANYHRVHASFDMKIESAVYLPGTLRPVKEKT
                     INKRNRVYCRNERIVLFGNSEYGKFACVLVGALFVGKVKLSFDSELQTNIKRGIVAEK
                     KYKKEVVIKKGEEIGYFEMGSSVILLLESDIFENMKLELKTSLKMGQSLF"
     gene            complement(14560..16089)
                     /gene="dnaB"
                     /locus_tag="PKN53_02795"
     CDS             complement(14560..16089)
                     /gene="dnaB"
                     /locus_tag="PKN53_02795"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOD87912.1"
                     /translation="MAINNKKQSSSKSEANIDSLNAIYGKMPPQATDIEKAVLGALLI
                     QSDAVFNILDILKPESFYDPAHQEIFQSIRDLGTNHKPVDIITVAHELKSRDKLDIIG
                     GEYYLADLTDKVATASHLEYHARLVHQKYIQRELIKASAEIQKRSYDESQDVEELINF
                     AESEIFNISEGNIKSETVKLDTILGKALEAIDEAAKNKNKLLGLPSGFSDIDRITSGW
                     QPSDLIIIAARPSMGKTAFVLSMARNMGVDHNKPIAIFSLEMSSKQLVNRLIAAESEI
                     SSTKLRTGDLQDHEWTQLNQKVKNLENVPIFIDDTPAISIFELRAKCRRLVRTHNIQG
                     IIIDYLQLMTTGVDMKGNREQEVATISRSLKAIAKELNIPIIALSQLNRSVESRSGDK
                     RPQLSDLRESGAIEQDADMVLFIHRPEYYGFMTNNDGESLKGLAEIIIAKHRNGATDT
                     VKLKFKKDFAKFSDYDRFDMSDTSAIFESKMNDSAFDDGNTFESNENFDSDFLSPNDE
                     F"
     gene            16319..16975
                     /locus_tag="PKN53_02800"
     CDS             16319..16975
                     /locus_tag="PKN53_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD87913.1"
                     /translation="MIKIENIYKSYDNLEVLKGINMQIAKSEIVSVLGASGAGKTTLL
                     QIIGTLLKPDKGSVFINETNVVGLKDKKLANFRNQEIGFVFQFHHLLPEFTALENVCI
                     PGFIAGTNKNELIKKAKYLLAALKVEDRAEHKPNELSGGEKQRVAIARALINNPSIIL
                     ADEPSGNLDSKNAKDLYEIIFGLREEFAQTFVIVTHNIEHAKLTDKIFTIKDGVISED
                     "
     gene            complement(16972..17718)
                     /locus_tag="PKN53_02805"
     CDS             complement(16972..17718)
                     /locus_tag="PKN53_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HOD87914.1"
                     /translation="MKYLIFIIAIVLITLNTNAQELSGTIKYEIKHDWVKKINSVKYL
                     SQSEKDKASYMWGTHSEYTEYAVLCFDKNTSRYEKIEDENKEGSWAWRSEEYIIYRNL
                     EENTTYDLIREVNKLFVIKDEIQNPTWKILNNIKEVAGHICMDAMYIDPIKGNKIVAW
                     FALDMPVPFGPDRFGGLPGMILEIDISEGALVMTATKIENKDVSEFLVKPKHKRKVKI
                     HNEEYYTKLISDQVEQAKKAERPYFWQIRY"
     gene            complement(17743..20580)
                     /locus_tag="PKN53_02810"
     CDS             complement(17743..20580)
                     /locus_tag="PKN53_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOD87915.1"
                     /translation="MRHLIKSVFLALVLVSFSLIGKAQVSQITIKAQIIDENDEIVPA
                     ATVMLLNSKDSTLINYTTSDSQGNFSFKNVKNSGYLLKISHVSFMPVQKNIPVSNQAT
                     VDLGIIKLEPIAEILMEVVIKSAQAPIFIKGDTVEYDARLFKVPPGSTVEDLLKRLPG
                     IDVDVSGNISSQGKDIRRVYVDGKTFFGDDPKSATKNLDAQAISKVQVYDDKSEQERL
                     TGIADGNKEKVMNLELKDEFKKGHFGKATLAGGFDDYRKKLLWASKANFNRFNEKHQL
                     SIIGFGNNVNQTGVNWDDYSEFKGSAAFSNRDNGDFGFGGDGSGFHVMEIGGGGIMGY
                     FDGKGFTKNYGGGVNYNYNTKKINFNASYSYNQTDLNSETFANRQTFLTDTSYLLNTN
                     SASDVFRNSHNISSRIEHNIDSSNTLIFKIYGRFSGNRSIDTRTQLFKTILDQNINKN
                     FTNETDTLDSKVINTLAIYNHSFKKRGRKFAISGAFNYYQSGRMENLINKNEFFSATT
                     ELEQINILNSKDNDDNTIKSSVLYVEPLSKRFSIFGFYNFRNYSTQSKNLGTDALTNY
                     IMVDSLSIYYQNKEMFNRVGTSINYNWEGLNISLGGAFQSINLFNKYSVTENSPLLVP
                     ELTKTYNNFIPNFTTYYEFPNNLHINLGYSYDIDVPSISYLQPIPNLSNRFYKTEGNP
                     DLEPESYHSTDISWTYWNQASFTNIHASVYYYYYDSQIVYNQETKFVEGLGYTTIMKP
                     ENLSGGQSVTAYLWSSMPIVKTILTLRLGGSYSYRESPVYINSIRNTTNTNSYRLNLG
                     FNLTLSSKLSLSLGSSGSLSDVSYSIQTSQNQNIINLSASTGIKWQFAKKSFFEANYD
                     FTKYQNDRFELNEVLHLANASIRQILGKNNKFELRLAVFDLLNQNKMISQTPGSNYIE
                     TSISPTLARYYMLSVSYNIKGFETKKKQMWW"
     gene            20913..21710
                     /locus_tag="PKN53_02815"
     CDS             20913..21710
                     /locus_tag="PKN53_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4065 domain-containing protein"
                     /protein_id="HOD87916.1"
                     /translation="MKTNLSQKQIGQRISELRKTRGFTQEELANRLEISRSSLAQIEL
                     GNRNVDILELQKLSMILKFSLDDFMSKNFSAIKNIDVKEEKEEKKSGERISVPKLQVN
                     KFKNILLYILERCAGKPNVGETVLYKLLYFSDFNYYELYEEHLTGAKYRKLPYGPVPQ
                     KLDAIINQMLEKGQLQKVKTEYHGYPQTRYLPLVKADLTKLKASEKEVIDGVIEQMSD
                     WSATMISEYSHKDLPWEVTEEGDYISYELAFYRELPYSVRIYEDEND"
     gene            21714..22043
                     /locus_tag="PKN53_02820"
     CDS             21714..22043
                     /locus_tag="PKN53_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87917.1"
                     /translation="MDFNQLPEFEKDLKFLLKKYRTLNEDLNEIKTILRKKPLERQPF
                     SFRIDNLSLETRIIKVRKIACKSLKGKGVNSGLRLIYAYFPNEEKITFIELYHKNYKE
                     NEDRQRI"
     gene            22556..25471
                     /locus_tag="PKN53_02825"
     CDS             22556..25471
                     /locus_tag="PKN53_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOD87918.1"
                     /translation="MQILEALKKEIQGEVQVDNIYITLYATDASAYREIPKAVVFPKN
                     KDDLIAIVKFANKHNLSLIPRAAGTSLAGQVVGNGIVVDTSRAFNKILEINKPEKWVK
                     LEPSVVLDELNKVLAKENLFFGPETSTSNRCMIGGMIGNNSCGAHSLIYGSTRDHVLE
                     MDCILSDGSEAHFGEISKAEFEEKCNLQNLEGEIYRQLKTLLTDPIILEEISKQYPDK
                     EIKRRNTGYALDLLADSEIFGNSNEKFNMCKLLAGSEGTLAFTSSVKLNLVDLPPKTK
                     ALMCVHTKSLHEVFEANLVALKHSPASIELMDKKVLDLSKANIKQNRNRFFINGDPAA
                     ILIVEWFADDIETINKQADELEKELRSKSLGYDYARVYGGDIARVWELRKAGLGVLSN
                     MPGDAKPVPVIEDTAVRPEDLSAFIKEFEELMQKLGLDCVYYAHIATGELHLRPVLNL
                     KDKKHVELFRTVALETAKLVKKYKGSLSGEHGDGRLRGEFIPLMMGEKLYDVFKEIKN
                     VWDPKNIFNPNKIVDTPPMNTSLRYKPGVETKEIKTIFDFSSTGGYMRAVEKCNGSGD
                     CRKSHIIGGTLCPSFQATKDEKNSTRARANMLREVLTNSTNNKPFNSKELYEVLDLCL
                     LCKACKSECPSGVDVAKLKMEFLQHYYDTNGVPLRSFAVAYLPVVYKIFASLPGFVNI
                     FLKNKFFSKILKSIIGFNQKIEFPLMHKTGFKKLYKGKQKFENPNPIKTIYLFNDEFT
                     KYLESDLGIKTILFFEKLGYQVKLAPINFSGRTWLSKGLVRRAKKLANKNIEILKNII
                     SSETPLIGIEPSAILSFRDEYPDLVNAENKNAAQDIAQNSFLFDEFIAAEFEKGNISA
                     NSFTDAELKIKFHGHCQQKSLITTKFTKQILSIPKNYKVAEIPSGCCGMAGSFGYEKE
                     HYDLSQKIGELVLFPEVRNADDDTVIVAVGTSCRCQIESGTGKKSLHPIEVLWEALV"
     gene            complement(25477..26286)
                     /gene="kdsA"
                     /locus_tag="PKN53_02830"
     CDS             complement(25477..26286)
                     /gene="kdsA"
                     /locus_tag="PKN53_02830"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOD87919.1"
                     /translation="MLKVENLILDNSAHFFLIAGPCVVENEEITLHTAKVLKDLCKKH
                     NIQLIFKSSYRKANRSSVNSFTGLGDEKALEILSKVKQELKLHICTDIHSPEEAEIAA
                     KVADIVQVPAFLCRQTDLLIAAGKTGKFVNIKKGQFLSPESMKFATEKILSTGNKNIM
                     LTERGTTFGYNDLVIDFRGIPKMKTFGFPVVLDVTHSLQQPNQSSGVTGGQPEMIEYM
                     AKAGLAVGADGLFIETHPEPKKALSDGANMLKLDLVDDLLEKLVKLRKALK"
     gene            26363..27112
                     /locus_tag="PKN53_02835"
     CDS             26363..27112
                     /locus_tag="PKN53_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HOD87920.1"
                     /translation="MYRISLVSYSNTLPFKEALYNSEFVAKNAILQERIPAMCAEDIF
                     TGEADISLVPIGAFPIPENTRIISDFCLAAKNKVESVLLVSQKTKEEIKTIILDYHSR
                     SSVKYIKILAEKFWKIKYNYQNADGNFKDLIKEDTAAVIIGDRALNFRNSIKYKYDIA
                     EEWFKFTGKPAIFAVWVANNKVSDSFLKEFNKILKSGFENRKQIAEKNKHLYPNFDLV
                     NYLTNCMDYNFDEAKQESLNMFNQFYSEILR"
     gene            27161..27577
                     /locus_tag="PKN53_02840"
     CDS             27161..27577
                     /locus_tag="PKN53_02840"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD87921.1"
                     /translation="MNLQIKDFEPKHYDEMNVLWQLTDMGGAYRGDNLEIILNSIKMG
                     GALYLLFNDDVMIGTSWITNDGRRLYLHHFCIHPELQGKGLSKLLMDKSMEFARKVGL
                     QIKLEVHREHVVARNLYDKYGFKYLGDYDIFIVREY"
     gene            27582..28868
                     /locus_tag="PKN53_02845"
     CDS             27582..28868
                     /locus_tag="PKN53_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOD87922.1"
                     /translation="MRKQILNLAYPAIISNITVPLVGLVDIALVGRLGSAAYIGGVGF
                     ASVVFSFIYSGLGFLRMSTNGLTAQAYGASNFREANSILIRAIILGVLSGLLLIALQT
                     PLERLLISMFDSSPESLKYASEYFYVRIYATPASLAVIGFMGWFVGMQDTKTPMWVTL
                     IISLANIAFSLFFVIYFKMGVKGVALGTVVAQYLGLISSILFYVKKYKSYLSYIDKKS
                     ILKFDEIKKFFNISKDIFVRSILLTGSFFLFNIVSADFGDEILALNSVMLQFLWFFSY
                     FADGFANAGGSIAGKYKGAANPKLLKETIKESLRLGLLVAIIFSVVYVFFSKQIVQIL
                     TDDVQVISLFETYHFWVWLIPITGFAAFIYDGIYIGITESKIMRNVMIISILIIYVPL
                     LFLAKHYIGNHGMWAVLILMLLTRGIGLKVQLKVKS"
     gene            28915..29388
                     /locus_tag="PKN53_02850"
     CDS             28915..29388
                     /locus_tag="PKN53_02850"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HOD87923.1"
                     /translation="MVIEKKKIAIQDTYGERFQHCWGCGPKNEEGLHLKSYPNEDGSQ
                     CICRFTPADKYTGGVPDNLFGGMIAVIFDCHGTASAAWFAHNKKGLELTENTVIGRFV
                     TARLEIDFKKPLPMNEEITVTSILEEIGERKAIVNMKMEAGGLVCAKAKMIAVSL"
     gene            complement(29473..29628)
                     /locus_tag="PKN53_02855"
     CDS             complement(29473..29628)
                     /locus_tag="PKN53_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87924.1"
                     /translation="MDIINSLNFDETHKLTPLYKLLTDFIVLPYGQTIDNQILAFFIN
                     FMNFENF"
     gene            complement(29756..29974)
                     /locus_tag="PKN53_02860"
     CDS             complement(29756..29974)
                     /locus_tag="PKN53_02860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOD87925.1"
                     /translation="MSVMNNIAEGFERKSNNEFKHFLFIAKGSCGEIRSMLYLAKDLN
                     KISDDDFKLLFAMSEEVSKMLSGLIKCL"
     gene            complement(30171..30929)
                     /locus_tag="PKN53_02865"
     CDS             complement(30171..30929)
                     /locus_tag="PKN53_02865"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOD87926.1"
                     /translation="MKKYRYLFLIIVVFSLGFNACDTDKNRCMKSTGEITTEKIYLDD
                     FNKVQIKSDFEITFITGSEHILSINCGENLIPFISHEIEGKELILENRNTCEWLRKRI
                     KPKLSITCPNLKEITINETCDLKTLDSLYFNTLSIQNWAGILTTDMILSGDSLYFRSH
                     ASTGDYKLEGRCNYAYIYNVGNGYFKGRKLFCKDIHAVHKSLGDSEINPIKKLIIEDI
                     KYGKLYSFGTECPEIISYAEEGWQEKFENIGCSK"
     gene            31060..34053
                     /locus_tag="PKN53_02870"
     CDS             31060..34053
                     /locus_tag="PKN53_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOD87927.1"
                     /translation="MWRMSFVIFLTSVLCLVTTSFNYKTNDFPTKRTTKPPSFLDENN
                     PWVDSVFNTLTQDEKIAQLLMLPAYSNKGKSHEEHILSLIKNYKIGGLIFMQGGPHRQ
                     AVLLNKYQSNSELPLLISMDAEWSLSMRLDSTVLYPRQMMLGAIQDNELIYEMGAEFA
                     RQLKRVGVHVSFSPVCDVNNNPLNPVINDRSFGEDKYNVSIKSYYYMKGLQDNGVLAC
                     AKHFPGHGDTHVDSHKALPVINHSFQRLDTLELYPFQYLIDKGIASVMVSHLFVPAID
                     SSKNLPSSLSPKTINELLKTNMGFKGLVFTDALNMGGITGGFKAGEADVKALLAGNDI
                     LLFPNEVPLVIEKIKQAIENGEISQEEIDNRCKKVLMAKYWVGLNKIEEISLENLHSD
                     LNNTEALLMKQKLIENSLTLVTNKNSLIPLQRLDTLKIASISFGSNNMSSFQNRLKYY
                     AEVDEFNFETEIKKSGSQGFKAKLKKYNLLIVGIHNTNSLASRKFGISKQTTEFVDEI
                     CAENNVILDLFANPYALAYFKNAEKCAAIIVSYNDWTLTNDLSAQLIFGGIPALGRLP
                     VSVGNKFSVNSGYDTKKIRLKYTSIPEEAGVDSENLYKVDSMMNEAINAKAFPGGQIV
                     AARNGIVFYIKSFGYHDYSNKIKVNDFDLYDLASVTKVVGSAPALMKLYDEKLFALND
                     KLSKHIPILQNTKKADFEIFDILTHRAGFKSWEAFFKKTIKNDSVQNLIYSKEKAENY
                     NTRVCEGFYILDSYRDTIFQDIINSPHNRYGRYVYSDMGFYFFPEMIKKFKGKSMDEY
                     LYDEFYSRLGAWTLRYSPLEKFSKKNITPTEEDNVFRKEQLHGYVHDQGAAMLGGISG
                     HAGLFASANDVAKVMEMFIEKGSYGNFDYINSETIDLFTKYQFDSLINRRGLIWDKPV
                     PNDTTKGLGTGSSSNLAYGHSGYTGTMVWADPHYDFIYVFNSNRVYKDAENWKILKLN
                     TRTIIEEIFYQAIIKKNPDLKYVKN"
     gene            34105..35274
                     /locus_tag="PKN53_02875"
     CDS             34105..35274
                     /locus_tag="PKN53_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOD87928.1"
                     /translation="MTDSSRYNLRGVSASKEDVHNAIRKIDKGLYPKAFCKIIPDILA
                     GDENYCNIMHADGAGTKSSLAYMYWKETGDLSVWKGIAIDAIVMNIDDLLCVGATDNI
                     LLSSTIGRNKNLIPGEVISAIINGTQEFCDEMGKHGINIVLTGGETADVGDLVRTIIV
                     DSTVTCRMLRKDVITNENIQAGDVIVGLSSTGRASYENFYNAGMGSNGLTSARHDVLA
                     NYLAEKYPESYDNAMPAELVYSGKKKLTESLEKPYSDVGKMILSPTRTYAPVIKEVLK
                     HYSNQIHGIIHCSGGAQTKILNFVDNLHIIKDNMFEIPLLFQMIQEQSNTDWKEMYKV
                     FNMGHRMELYLPENIANQVVEISKSFNVDAKIIGRVEKSESKKLTIKSEKGEFIY"
     gene            35285..36373
                     /gene="prfA"
                     /locus_tag="PKN53_02880"
     CDS             35285..36373
                     /gene="prfA"
                     /locus_tag="PKN53_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOD87929.1"
                     /translation="MANDLILDKLRDVKKRFLEVGNLLTDATIVSDMDKYIKLNKEYK
                     DLQPIIEAYDEYVLIISNIEDAKNILANEKDSELREMAKMELDDLQPKLEPLEEKIKL
                     LLIPKDPEDDKNAIIEIRAGTGGDEASLFAGELMRMYVKFAESKNWKTEITSYTEGTV
                     GGYKEVVLSMTGNGVYGIMKYESGVHRVQRVPQTETQGRVHTSAASVAVLPEADEVDI
                     KLNPADIRRDEFCSSGPGGQSVNTTYSAIRLTHIPSGIVVTCQDEKSKLKNLDVAMKE
                     LRTRLYNLEHQKYLDEIAKKRKTMVSTGDRSAKIRTYNFPQGRITDHRINYTMYNLQN
                     FMLGEIGELIDHLIMAENAERLKEANEE"
     gene            complement(36960..37523)
                     /locus_tag="PKN53_02885"
     CDS             complement(36960..37523)
                     /locus_tag="PKN53_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87930.1"
                     /translation="MTYEQYIDIRAEYISANPNLWVFEISAPRGSGEKFAQTYVQGKC
                     SKLKSPNKKLDPNYSGDYDLWLDGIRIEVKASRAVDSDSEEPLYMKALSRKTDKNFLM
                     NFQQLKPQCCDVFIWVAVYRDQIVLWVMSSKEVLENPSYSKGQHRGNKGNEGQLHIRQ
                     DNVAQLEKYELQGDNLEQAIRDAAKRN"
     gene            complement(37653..39281)
                     /locus_tag="PKN53_02890"
     CDS             complement(37653..39281)
                     /locus_tag="PKN53_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOD87931.1"
                     /translation="MQTNIFGEIETYGLTIEQAAKTANVSTATIRNWIKTGYLFQSGK
                     GIVSQESFEKFMSEVAGKEKLNARANKLLKDDHDHQEVSDKLSLLIEKFNGEQIGIEY
                     ESLLADSYRNKEGIYYTPSWVVKDMFKNIKINPDSTFLDPCCGSGNFLIEAIRQGVAP
                     ENVYGFDVDENAVLIAKQRIKEEFGIDTHNIKVGDFLHEAHKLKTEKITYDLIFTNPP
                     WGKKIEKSAKEKYSALYSCGNSLDTTSLFLAASLSILKVNGFLGFLIQEAFFNITTFE
                     DIRKRVLAKKILRFVDYGKAFKGLVTKAQAIVIENNASDPNDIIKCCSENNVFNRTLD
                     SFKKNPKHIFNFWTNETEAKVIKQLYSIKHITLKDRANWALGIVTGNNDRFCSNEPQD
                     DYVPIYKGSDITKLGLKEPSTFIFSDFSNFQQVAPLEMYHSKEKLIYKFISSDLCFYC
                     DTKQRYILNSANLLIPLNIGISAEQLTMLLNSEIINWLFKKLFSTHKVLRSDLELLPI
                     HHEYFSFNQEFTETDYLNYLQIIKTSDGTYRVKD"
BASE COUNT        13309 a         5887 c         6433 g        13807 t
ORIGIN      
        1 aaattcacta attaatctaa caccataatt aaatttttta aatgaagttg actcctgtgc
       61 taatgtaatc gatgatgttc ctatcactaa gaatagactt gcaataatga gttttttcat
      121 aatgcttgat tttattatta ctacaaatat aaagcaaaac aaaattattt ccaaattaat
      181 aatgtcttgt ctttcctttc attaaattag cagctaagca ggaatgtaca gggataattt
      241 caattaaatt tcctggttta tagttttcaa aatttgaatt attaatttta acaattccat
      301 gttcttgact taaagaaaca atatcttcgt taatttctga aatgtctgtg ctgtaatgtt
      361 ttattgctct gccaaaaatt ttcttattat taaattcaat aaattctttg ctaaaatgaa
      421 tagctccgcc atgtattata atttcttttc tatcattgtt aatgtccaca ataggacaaa
      481 ataatgtagt agcaatttgc tctttggtgc aaacttgttt ttctaacatc ataaggtcgt
      541 aataaacaaa atttccaggg cggatttcgt ctataccatc aaaattgtct aataagctac
      601 aacctggtgt gtcgccaatg gaaatttttg gcgaatagtt tgaaaattca gattttagga
      661 aattcatttt agctattgag cccaaagttg attgcaaaat ttcctctttt ttatttagta
      721 aatatgtttc gccattgtga tttagaaaac ctgaaaaatt aagatgtgaa gcttgcgtta
      781 taagttttat acaatcttga atttctttta aattattgtg tgaaattcca gatcgcttat
      841 agccagtatc aatttctatc caaacgtttg cttttatgtc attaagagaa gataattgtt
      901 ttgcagaaaa agaatttgaa accaaaagat gcaaatttgt atttttagat aatttttgaa
      961 gtgctttttg ttcacgaaaa tttattggaa aagaaatatt gatgtcttta aaacctaaac
     1021 gcctaaaata tatagccatt tcaatagatg aaactgtaat tttatttatt cctaattctc
     1081 taaaaatatt tgcagtttgt ttattgtcgt gggttttaaa atgaggacga aaaatagttg
     1141 tttctccaag tttgttttgt attttttgaa tgtttttttt gactatatct gtgttaacag
     1201 cgaaagttgg agattttatc ttgttaattt taagcatttt caaaaagttt tatttgcaaa
     1261 ttttgtaatt ttatatgact tttcaaataa ttaaaaaata ataaaaatat ttacgattaa
     1321 ttatgaataa aaattaaatt ttcaactttt tattgttata tttgtttaat tattgatatt
     1381 atgtaacact tcatgaaaag tatatttatt tttaattaaa atttttaaga aatgcaaaac
     1441 aaacgaatag ttgtgattgg cggttctgct gctggaccta aagtggcttc tcgcgcaaga
     1501 cgtttagatg aaaatgcaga aattactatg tttcaaaaag atgctgactt atcaatggca
     1561 tcttgtggtt atccttatta tgtcggcgga ttttttgatg atagaagtca gctcttatgc
     1621 tctccagctg gtgttgtaag agattctaac ttctttttaa atgcaaaaaa tattgttgct
     1681 aaagtaaata ctgaggtaac aaaaattgac agaaaagcaa aaaccattga ttatcgaaac
     1741 ttgataacag gcgaaaagtc aagcatggag tatgataaac ttgttattgc aacaggagca
     1801 acacctcgaa gaccaccaat tccaggtatt gagttggaag gcatttctac tttatcgtca
     1861 atgcccgatg ctgattattt acgcaaggta agtgatgaag gaaaaattaa aaaagctgtt
     1921 gttataggcg gtggtttaat tggtattgaa accgtagaag ctcttcatct tgcaggaatt
     1981 gaagttactt tagttgaact tttacctcaa cttttgactt tccttgatag acatttggct
     2041 cgcttggtac agcagcatgt tcaatctaaa gctaatgtga ttttagaaaa tggtgtttct
     2101 gcttttattg gtgaaaatgg aaaattaaca gcagtaaaat taaataatgg caccgagtta
     2161 ccttgcgatt tagcagtttt agcaattgga gttactccaa attcaaaatt agcaaaagat
     2221 gcaggtttag aaattagcga aagaggtgga gtagttgttg atgaatatat gcaaacttct
     2281 gacccagata tttatgctct aggcgattgt gttgaaatac caaatttaat tacaaaaaat
     2341 aaagttcacg caccatttgg agatttggca aacttgcaag gacgtgttgt tggcgaaaat
     2401 ttaattaaag gaaatgttgc aaaatttgaa ggaacaattc aaacaggtgt ttgtaaagtt
     2461 tttgattatg ctgcaggaac aacaggcttg aatgagcaaa atgcacgcaa aaatggattt
     2521 aatgatatag aaactgtaat aaatgtaagt ttggataaac ctggatttat gaatggtaat
     2581 ttattgatta ccaagttggt agttaataaa gctgatagaa aaattttagg agctcaagta
     2641 ataggtcctg gcgatgtttc taaacaatta gcaatttggg ctattgcaat taaaggcaag
     2701 ctaacagttg atgatatgat taatgcagat ttaccatatg caccaccatt ttctttagct
     2761 atcgaccata gtatcgctac tgcacatatt atgcaaaata aattaagagg acttttcaaa
     2821 ggtatttctt ccgaagatgt agaagaaaaa gtgaaaaata aagaaaatgt tgtttacctt
     2881 gacgttagaa atccaaatga atacgaacaa atgcgtatag gtattggcga aagacttatt
     2941 cctcttggtc agatacgaaa aagaatttct gaattgccag aagacaaaaa tactgaaata
     3001 atagtttggt gtaaaatttc gttgagaggc tacgaagcag ctcttgtttt acaagcaaat
     3061 ggctatacta atgtgaagct tatggaaggc ggagttatgg cttgggctgg aaaacgggaa
     3121 aaataatgtc agttaaaagt taaaagtcga aagttaaaag aggctgacgc gtctattgct
     3181 ttgcagagtt cgcttcgctc actctgctcg ctaatgggtt gatgtgtctg aaaagtgcga
     3241 catttaattt ttaattttta actttcgact tttaactttt aactgattta agatatccaa
     3301 gtaataatat attctaaatt tccgatgttt gggtcggcga tatgccattt gccttgaaca
     3361 tttaattctt cgagagcaag tttaaaattt gctagagcta tccccatatc aactttttgc
     3421 aaatcgctgg ctttagttgt gccaacaata gaatctcgtt ttaagtaaaa atggtatttg
     3481 tcttcaattt ttaaaactcg ccaaggttgc ttattcattg ccgaaggagc ttttctaaca
     3541 gcttctaatg ccttgtgata attgtcttcg ggattaaaaa ttattggagt ttcaaaatta
     3601 ttttcaaaaa agaatgtgtt aaagtctttt cttaaacttc catctgaaaa aactaaacct
     3661 aatctttctc ttcgagattt ttttgttgat ttataaccta caggtgtgat acatggaata
     3721 atttcatttt tttgtgtgtt tagtaatttt ctataatctt ttctacgaaa actaccacca
     3781 agccagcaag ttcctaagcc taagcgagtt aattctatta ttattctttg caatgaatat
     3841 ccataatcaa cttctgaaaa tgcagatttt tcaatgcaac caccaataaa ataagcagca
     3901 ttatcaataa agccgtaagt tcctaatcta actttttctt gttctctagc tttaggtttt
     3961 tcaataagaa caaatctagg tctattgtca aatggtccta tttttggtgt agataaaagt
     4021 tcgtttatct tttcaattaa attactgtct aatttttctt tagaataatt tctaatagat
     4081 cttcttatgt ttattgcttc aatatgtcca atcattacaa taattttagt ttatttgttg
     4141 ttaaaaactg aatttaaaac ttcgccagta gaagcaggtg gagcaggagt gttaagtatt
     4201 ttgcttatag ttggagctat gctagtaata tttacgttgt taaaaatatt ttgtttttta
     4261 actttccaac cataccaaat tagcggaaca tttgtatcat atttataacc agaattgtgg
     4321 ttgcaaacaa aaggaatatc ttctatccaa cctgctttta gtgaaatcat tatgtcgcca
     4381 gagcgttttt ggttgaatga gttttgcatt ttttttggca ttccttgagt gaaaattata
     4441 ttttgaaatt ggtgtgaact tattgcattt gaaattcctg cagaattaat tataaaagaa
     4501 ataacctttt cttgaaattc gtttaatgaa atttgcgaat cttcaatcag tgttttgttt
     4561 aaataaattt gattattgtt atagtctaac acccaatcgc cttcgccgta tatggctttt
     4621 aaataggagc gaagcaaagt taaaatataa tgttgtttaa aaactcctgt aggcatttta
     4681 ttatcagcta aatattctgg attttctgat actccatggt ttgaagttaa atatattagt
     4741 acattgtttt ttccaacaac atcttctatc acacttatca aatgtcctaa atctctatct
     4801 agtcttagaa acaagtcttc aacttccatt gattgtggtc caaatagttt accaatattc
     4861 tcacttaccg aataatttat aaataaaaaa tctgtaaact tatcttttcc taattcttcg
     4921 ttgtagagtg ccgcaatagc catatctgtc aggtgggttt gtccttcagg tattagattt
     4981 agtaattcgt aatcttttat gcttttgcta atttcttcat atttataagg aaaagtttta
     5041 taaaccatat ctattccata ttcataaata ctagaatcgg cttgagcagt attgtatttt
     5101 tcagtttcta aaagtggcga ccattctctt tgcaagtaat catcgggcat ttttttgtcg
     5161 ttttgttctt ttacccatgc aggtagctcg ttcatataat aagtagaact tacccaattc
     5221 ccagtttttg tgtcaaacca atatgcagca tcagcagtgt agcctcccga tataattgca
     5281 ccaatagggt ctaaagaaac acttattgtt ttagattttc cttgtccaaa gagtttggct
     5341 tcgtcgctaa atgttgttgt taaaatattt tttggcgagt aagaaaaatt gcttgggttg
     5401 cctccaatag ttttgtaaga tttagtttct atacaatgtt ctttattgcc agttaaagca
     5461 ttaaaccaat aatctgatac aataccatgt tctgaaggtt cgcagcctgt aactattgtt
     5521 gcatagcctg gtgctgcttg cgttaagcta tattcatagt ttgcattttg acaaaaactt
     5581 ccatttattg ctaatttttt aaaacctttg tcgcctaaat tattccaaaa tctttctaaa
     5641 taatcctgac gcatttgctc cacaacaatt ccaacaacaa gttttggctt gtcagaagga
     5701 attacacttt cgatttgaga tatcgaactt gaatatattg caaataaact tagtgctagt
     5761 aaaattattt ttttcattac ttattatata ataagtggca aatataaaat tgttttaaat
     5821 ttttttcttg aaattcaatt ttatttatga atagaaaact gttaatacaa ttattgcaac
     5881 atcgaaattg ccttttttaa atttgttatg aaatagtcta tttcttcgaa tgtattgtaa
     5941 aaagcaaaag aaactcttac tgttccacta atgccaaatt ttttcataac aggttcagca
     6001 caatgagttc cagttctaac agctattcca gctctatcca aaagcgttcc tatatcattg
     6061 taatgtatgt tttttacgat aaatgaaata agtccagatt tgtgttcagc ctcgccgata
     6121 atctttaggt tttctattgt ttttaattgt tttgtacaat attttaaaag ttgattttcg
     6181 taatcttcaa tattttttag tcctatttca gaaatatatt ctatagcttt tccaaatgca
     6241 atattgtcga tatagtttgg tgtgcctgct tcaaatttaa aaggcagttc gttataggtt
     6301 gttttttcaa aacttacatt tgcaatcatt tcgccaccgc cttggtaggg gcacatttga
     6361 tttaataatt cttcttttcc gtatagaact ccaactcctg ttggtccata agctttgtga
     6421 gatgaaaaaa cataaaaatc gcaatctaat tcttgaacat caatttttag gtgttgaact
     6481 gcttgagctc cgtctattaa aattttgata ttgtttttgt gggcaatttc tattattgtt
     6541 tttataggat ttattgtgcc aagtgagttt gatacatgag ctacagaaat tagctttgtt
     6601 ttgttggtaa ttaaattgtc taactcttta atgtttagtt cgccaatttc gttaaatttt
     6661 aaaactttaa gttttgcttt ttttctttcg cacaataatt gccaaggaac aatatttgag
     6721 tgatgttcca tttcggaaac aataatttcg tcatcttcgt ttatgaaagt ttcgccaaaa
     6781 gaaaaagcaa ctaggtttat tgaatcagtt gtgccctttg taaaaattat ttctttttct
     6841 gattttgcat ttagaaaatt ttgaataatt tttcttgcat tttcgtttgc atctgtagca
     6901 aaattgctta aaaaatgtac tccacgatga atattagcat tatattttga ataatattca
     6961 ctaatagtat caattacttg ctttggcttt tgcgaagtag caccattatc aaagtatata
     7021 agatttttat tataaacttt ttcgtttaaa attggaaaat ctgaccgtat tttatttata
     7081 tccaaaatcg aattattttt ttgcaaaatt ataaagttta aggagaaaaa aaaatgccca
     7141 taaaaatacg gacatttttt taagttatta ttatttagtt ttgtttattc aacaataagt
     7201 ttttgagttg ttacatcatt tgcagtataa actttgataa agtaaacgcc agaacttaga
     7261 ttactaatgt taatttcggc attgttggta ttaacatttt caaaacttac aatttgtcct
     7321 gaaatattta aaatttcaat ataatttata ttttcttctg aatttacttt tactatgtcg
     7381 cttgctgggt ttggataaat tgataagctt tttgtaatat tgttttctat atttgaaggt
     7441 tctggcaaag caattttatt aacttgttga atttcacctg tacttcttct ttgaataaag
     7501 gcaacaattt cgcacttatt gataacataa tttgaagctg gggtaatttc aaattcacca
     7561 ctataagttg ttccagaatc aaaaattact gtgccgtttg catctgggta cattgccctt
     7621 acagcgttat ctactgtgct tagtcctccc catgagtatg gaatatttgt ttctgtcaaa
     7681 gctatcataa gtcttatttc atcatcataa tatccaaaag tttcttcggc attaacagta
     7741 agtttacatt tatatggttt ttcagaaatt atttcagttg ctaaatctaa ggtataaatt
     7801 gctggtacat caataaattc gtcataatag tattcgaaat attctttttg tgaagctacg
     7861 tctggagcgg atccagatag atattgtgtg ccatttataa cacttgcggg gtatccttca
     7921 ataggttcaa ttatacttcc ataataactt tctctagtag ctgttgctga agtttcaaaa
     7981 ctatcaccgc catgaaattt tataacagcc acttttttgt cagtagcttg tatttgagca
     8041 attgcttgtg caggtgtagg acagtagcca caccaagtac cagtaaacat ttcatacatt
     8101 acaaaatttc tttcaactcc accttcaatt gtaacattaa ggtttaaagg agcagagtat
     8161 ccgttgcttt cgccatagaa agctaaaagc gaatatttat tagtaccagg ataaacattc
     8221 aaatcagtta cagttgtttc tgttgtagtg ctgataattg tgccatttct tgcgatgctg
     8281 tagtttacag gtgtgccttc tgttggtgct tcccaagtta acaccacatc atttccattt
     8341 tcaatagtat agctaaagtt agttggagga tatggagttt caacttcttc aattaggaag
     8401 tcgtccatat aatattcaca agtacctcct tctttccagc cataaaaatc aaaggctcca
     8461 agtttattaa ctcctgttcc agtagctcct ttactccatt gataggcatg aactaattct
     8521 ccatttacat aaaaatcaac ccaatcgctg tttaagtcaa taaagtgttg gattttcatc
     8581 cattctccag gtgtataaga ataagttgct gcagatggtc catttccatc aattgtcata
     8641 gttccgtttt ttagaaaaac ttgcaatccc caaacaagtc cagatcctga agggttaaat
     8701 tgttgcataa tattccaata ggcttggcga ccagctggaa cgtaaagata aaactcaact
     8761 ctatagcgtc ctgtagtaag tccaccaaag tcaataactc cgtcattatc atttataact
     8821 ttaacagatt ttgttccaga atatgcttgt gcatctgaaa ctgtagggtc ttcagctgag
     8881 cctggtgttc cattccaagt gttccaccat gtactagatt cttctgcaat accattacca
     8941 acagtgtagg attcaaaatc gtcagaatac agcacttgtg cattaatgta attaatccca
     9001 agtagtagtg agattaaaat aagtagatgt cttttcatat catttcaatt taaaagtttc
     9061 tattaattat attattatca cttgtttagt tttatcaaat tgttagtctg ttttgattat
     9121 atattgcaaa tttagtaact tattttaatt gttcaagatt ttctttgaaa atttatgaat
     9181 ttattattaa ttattttagt ttatattaga aagtgttttt atattaaatt ctccttgttt
     9241 tctatattta ttcatcatgt gagaattttt ttaacatttc gcgatatgta gtaaaaactt
     9301 tagctctgga aacaaatcct acgtactttc cttcatcaac aacaggcaag ttaaaataat
     9361 ttgagttttc aaatatttct gcaatatctt taacatccat attagtattt accgtgtatt
     9421 ttggaatatc cattaaatct ctaacataaa cgctgtcgta aagttctgtt ttaaacatta
     9481 agtgttttac atggtctaac caaactaatc ctttaaagta gttgtcatcg tcaacaacaa
     9541 catatactgc tctgcttgat cttgaaataa ctttggttaa gtcttttaat tttgcatttt
     9601 cgtttatagt taaaaagttt ttttcaatca aagattctgt cctcattaaa gtaagcatat
     9661 tactgtcggc attgtgagtt agtagttctc ctctttcggc aagtaaaatt gcataaacag
     9721 atttaggcat aaatattcta acaacaaaga atgaaattgt tgaaactatc attagtggga
     9781 taaaaagttc gtatcctcct gtaatttcgg ctattaagaa aatggcagtt aatggagcat
     9841 gtataactcc tgcaataagt ccagccattc caactaaggc aaagtttcca tgagaaatgt
     9901 ttagtttcat agaatttgca acatgggcaa aaagaaaacc taagtttgct cctaaaaata
     9961 gagatggtgc aaatattcct cctgttcctc ctgctccaaa tgttgctcca gttgcaaagg
    10021 ctttagttaa tagtattaaa ataataataa tacatatgcc taaaaaactt tgccattgtg
    10081 aataaaatac tccatcaaat atatatgaaa tgtcgccgtg taatgctaaa ttaataactt
    10141 cgtatccttc tccgtataac gaagggaaaa gaaatattaa aacaccaagt attgtaactc
    10201 caacaataaa tctggcacgt tttgttttga gtttggcata ttgttttcct tgccacaaat
    10261 aaagctttga aaaataagta gaaagcaaac ctgcaataat acctaataaa atatataaag
    10321 gaacatcttt taaaaagaaa gggtcgatat ttgctacgtg ataaacgtag ttttgtccta
    10381 aaaataaata tgaagttaaa gctgcagaaa ttgatgccac aagtaatggc attaatgaac
    10441 ccattgttaa atctatcata ataacttcta gggtgaaaac tattgcagca attggtgctt
    10501 taaaaattgc agctatcgca cccgtacaac cacagccgat taataaaatt agctgtctat
    10561 aatttagtct aaaaaattgt ccaatattac ttccaatagc agaacctgtg ccaacaattg
    10621 gtccctctaa tccaacagaa ccaccaaatc caacagttaa agagcttgca acaattctag
    10681 aaaacatatt gtgtttttca atttttcctc tttctttaga aatgctataa agtaccatag
    10741 gaataccatg tccaagtttt ttcttaatga aaatatttac aaataaaagg gttaaaccaa
    10801 ttccaatggc agggaaaaat atatagatat aactaaaaga atacttatta tttaaaaata
    10861 ttgcgacttc tctaacaaag tgcactgagt tttttattaa aacagcagct aatcctactg
    10921 caatacctac aatcacagct aatattagca taaaattctt atctgaaatt tttctaactc
    10981 tccagataag tagtctcttt gcaagtttat tacaatatgt tttcatatta ttgcaaatat
    11041 gttaaaatac ttgtaattaa aaaatatttt taaatatttt tagttatttt tatccacaat
    11101 ttgtagtgag tttaatttag tcatattaat tatgaaaaag tataaaattg gaatagcttt
    11161 aagtggtggt ggtgtaagag gttttgcaca tttgggcgtt ttgcaagcct taaatgaaag
    11221 gggaattttc cctgaaatag tttctggaac aagtgctggt tctattgttg gagcttttta
    11281 ttgtgctggc tactctccac atgatgcact tgaaatcatg aagactaaaa aattttctga
    11341 ttttacgaaa atacatattc caaaagacgg tttttttggg cttgaaaatt taaagttaat
    11401 tcttgaaaac aatattaaac aacttaattt agaagatttg aatattccac tatatgtttg
    11461 tgtttcaaat ttgaatacag gaaaagtaga atatgtaaac aaaggtcctt tacatgatat
    11521 gattttagcg tcttcggcta tacctgtcgt tttttcacca gtaaaaatag gaaaaactta
    11581 ttatgtagat ggtggattaa ttgataattt gcccgtgaag cctttagtaa gattatgtga
    11641 tgatattatt actgttaatg tttttcctaa tgacgaaaat caaaaatttg actccttact
    11701 tcaagttgca atacgttcat ttcaaatgaa tatgaataat aatattaaat atataaaatc
    11761 taaatccact gtattccttg aacctgctgc tttatctaaa tatcatattt taaaaactaa
    11821 atatcaggaa gaaatttttg aaataggtta taaatatgct aaaaaagcaa agtttgagtt
    11881 aagctaaaat tttaatattt gatgatattc aaagtcgcaa ttactccagc cagcatcaat
    11941 ggttgctcca actttttcgt acatttttat tgctggagta ttccaattta aaacctgcca
    12001 gcgtaatcgc ctgcaatttt cttcttttgc agttttgaaa attagtttta ataattcagt
    12061 acctattcct ttgcctctat attgtggttt tacatataaa tcatctaaat ataatgattt
    12121 tccaacccat gtaaagtagg cgaaaaagta tagagccatt ccaacaactt ctttttcttt
    12181 agtttctgca acaaaacatt gaaataaatt ttgttcttgc ttcattattt ctgtggtatt
    12241 cagaacttta tcaggagcat tttcaaatag tgcaagctct ttaatcatct ctaaaattgc
    12301 agcaaaatca ttttcattcg ctaaacgata gtttatttca agcattttaa gtaaattttt
    12361 gcaaaaataa tattaaaatt tttatctctg taattttgtt ggagaataca tagctaaaaa
    12421 ataatttgta tcataagaaa taaattttgt gtgcattaaa aaatatatta tttttgcagt
    12481 ccaaattgtc aaatgtgtaa tggggctttg cagtggcaaa agcttagtag cacgcaaatt
    12541 tattgatatg aaacatttta aaattcaagg tactttacgt acagagcttg gaaaaaaagc
    12601 aacaaaagca attagaaaag gagaaaatat tccttgtgtt atttacggtg gtgagaaaaa
    12661 cataaatttt tacactccat tttctgcagt tagaaaattt gtttatactc cggaagtaat
    12721 gctagcagac atcaatgttg atggaaaaga ctatatgtgt atggtgaaag aaattcaatt
    12781 tcatccagta acagatagta tccttcatat tgatttttat caaattactc ctacaaaacc
    12841 tataattatt caaatccctg tgaaaactgt tggaaactct ataggtgtaa gatcgggtgg
    12901 aagattaaaa caaaactaca gaaaaatatc agttaagggg ctaatggatg atattcctga
    12961 ttattttgaa gttgatatta ctcctttgaa tataggacaa tctattagag ttagagattt
    13021 aaaatcagac aaattagaat ttattgatcc aaaagcaaac gtagttgtat cagttgtagt
    13081 aacacgtaca gtttcttcaa caggtgaaga agaaggcgaa gaaggtgcag aaggcgaagg
    13141 tggtgaagaa gaaaaaactg aaactgccga atagtcgttt aatgttctga actgtgaaat
    13201 atttaatcat aggtttaggc aatcctggag acgaatataa aaacacacgt cataatatag
    13261 gctatacagt gctggacact cttgcaaaga agtccagcac ttctttttct gataaaagat
    13321 acggctttgt tgctgatata aaacatagag gcaaaacaat gattttagta aaacctacaa
    13381 catttatgaa tttaagtgga aaagctgtag attactatat taagaaagaa aaagttcctg
    13441 tagaaaaaac tttgattgtt gttgatgata tagctttgcc atttggtagc ataagaatta
    13501 gaaataaagg cggaagtgcc ggacacaatg gcttagaaca tattgaatat acactaggaa
    13561 ctaatgtcta taatcgctta cgttttggga taggaaacga ttttaacaaa ggcggacaag
    13621 tagattatgt cttaggtgaa tggacagaag aagagaaact tttactacca gctagaattg
    13681 aattagcaat tgaagcaatc ctaagttttc ctattcttgg aatagagaga actatgaatt
    13741 tttataataa caaataatgt cgaattctaa aaataataag caaaattaca gaaatttaag
    13801 ttcgcgtttt ataggcaaat tttcaaatat aaaaaagcca agatgcttag taaacgccgt
    13861 aattaaattg tataaaagat attataaaat agatttgaat ggttttgttg ttcccgaaaa
    13921 tggatttgaa agttttaata gtttttttac aaggaaatta aaggtaggag ttcgtccaat
    13981 aagtaatggc atagtatcac caattgatgg tagtttattt gattttggaa aaatttcatc
    14041 tgaaaataaa atatatgtta aacacaaaca ttattttata gatgagttaa taattagtga
    14101 ttatgatggt ttaaaatctt atgctgtttt ttatttgtcg ccagcaaatt atcatagagt
    14161 gcatgcaagc tttgatatga aaatagagtc agcagtatat ttgccaggaa ctttaagacc
    14221 agtaaaagaa aaaacaataa ataagagaaa tagagtctat tgtagaaatg aaagaatagt
    14281 actttttggg aattctgaat atggaaaatt tgcttgtgtt cttgtaggag ctttgtttgt
    14341 aggaaaagtt aaattgtctt ttgattctga attgcaaaca aatattaaac gtggaattgt
    14401 agcagaaaaa aaatataaaa aagaagtagt aattaaaaaa ggagaagaaa taggatattt
    14461 cgaaatgggt tctagtgtaa tattattgtt agagtctgat atttttgaaa atatgaagtt
    14521 agaattaaaa actagtttaa aaatgggtca atctttattt tagaactcat catttggact
    14581 aagaaaatcg gagtcaaaat tttcattact ttcaaaagtg ttgccgtcat caaatgcact
    14641 atcattcatt ttggattcaa atatagcgct agtatcgctc atgtcaaatc tatcgtagtc
    14701 agaaaatttt gcaaagtctt ttttaaattt taatttaacg gtatctgttg ctccattacg
    14761 atgttttgca ataataattt ctgccaatcc ttttagcgat tctccatcgt tattagtcat
    14821 aaatccataa tattcaggtc tatgaataaa caaaaccata tcggcatctt gttctattgc
    14881 accagattct ctaaggtctg aaagttgagg tcttttgtcg ccagagcgtg attctaccga
    14941 gcggtttaat tgagataatg caataattgg tatgtttaat tcttttgcta ttgcttttaa
    15001 agaacgtgaa atagttgcaa cttcttgttc tctgtttcct ttcatgtcaa ctccagtagt
    15061 cataagttgc agatagtcaa ttataattcc ttgtatattg tgtgttctaa ccaatcttcg
    15121 gcatttagct ctaagttcaa aaattgaaat tgctggcgtg tcatcaataa aaataggtac
    15181 gttttctaaa ttttttactt tttgattaag ttgtgtccac tcatgatctt gtaaatctcc
    15241 agttcttagt ttagtgcttg aaatttcgga ttcggcagct atcaaacgat taactagttg
    15301 tttagaagac atttctaatg agaaaatagc aattggtttg ttatgatcaa ctcccatgtt
    15361 tcttgccata gatagcacaa aagccgtttt tcccatagat ggacgagcag caataataat
    15421 taaatctgaa ggttgccagc ccgaagtaat tctatcaata tccgaaaatc cacttggaag
    15481 acctagaagt ttatttttat tttttgcagc ttcgtcaatt gcttctaaag ctttgcctaa
    15541 aattgtgtct aacttaacag tttcactttt tatatttcct tcagaaatat taaaaatttc
    15601 actttcagca aaatttatta attcttctac atcttggctt tcgtcgtatg agcgtttttg
    15661 tatttcagcc gaagctttga taagttctct ttgaatatat ttttgatgaa ccaatcttgc
    15721 gtgatactct aagtgagagg ctgttgcaac tttatcggta aggtcggcaa gataatattc
    15781 tccaccaatt atatctaatt tgtcgcgaga ttttagttcg tgagcaactg ttataatgtc
    15841 aacaggtttg tggttagtgc caagatctct aattgattga aatatttctt gatgagcagg
    15901 atcgtaaaat gattctggct ttaaaatgtc taaaatatta aaaactgcat cactttgaat
    15961 taataatgct ccaagaactg ccttttctat atctgtggct tgtggaggca tttttccata
    16021 aatagcattt aaactatcaa tgtttgcttc tgatttcgaa ctgctttgtt ttttattatt
    16081 aattgccata aatatatctg aaattttact tcaaaaatac tgcttagaaa ttggcttaaa
    16141 aaattttttg acgaaaatat aataaacttt tatccacaat ggggtttgaa aaaaatgttt
    16201 ataacttttt tttgttttaa aagcttataa atgactatct ttgttaagaa aaaataagtt
    16261 tttaaaaata gttattaaga taaaatttta attttaactt tacgaaaata ttttaactat
    16321 gataaaaatc gaaaatattt acaaatctta cgacaattta gaagttttaa aaggcataaa
    16381 tatgcaaatt gcaaaatctg aaattgtttc tgtattaggt gctagtggag ctggaaaaac
    16441 tacgcttttg caaattatag gaacactttt aaaaccagat aaaggtagcg tgtttattaa
    16501 cgaaactaat gttgttggat taaaagataa aaaattagct aattttcgaa atcaagaaat
    16561 tggttttgtt tttcaatttc atcatttgtt gccagaattt acagcattag aaaatgtatg
    16621 tattccagga tttattgcag ggacaaataa aaatgaatta ataaaaaaag caaaatattt
    16681 attggctgca ctaaaagtcg aagatagagc agaacataaa ccaaacgaac tttctggtgg
    16741 cgaaaaacaa agagtagcaa ttgctagagc attaattaat aatccttcaa ttattctggc
    16801 agatgagcct tcgggtaatt tagactctaa aaatgcaaaa gatttgtacg aaataatttt
    16861 tggattaaga gaagaatttg ctcaaacttt tgttattgtt actcacaata ttgagcatgc
    16921 aaaattgaca gataaaatct ttacaataaa agatggtgtg ataagtgaag actaatatct
    16981 tatttgccaa aaatatggac gttctgcctt tttagcttgt tcaacttgat cggaaattaa
    17041 ttttgtgtaa tattcttcgt tatgaatttt aacctttctc ttatgttttg gcttaactaa
    17101 aaattcactc acatctttgt tttcaatttt tgtagcagtc atcaccaaag ctccttcact
    17161 tatatcaatt tctaaaatca tacctggcaa gcctccaaat ctatctggac caaaaggaac
    17221 aggcatatca agagcaaacc atgcaacgat tttatttcct tttatcgggt ctatatacat
    17281 agcgtccatg caaatatgac cggcaacttc tttgatatta tttaaaattt tccaagttgg
    17341 attttgaatt tcatctttta ttacaaaaag tttgtttact tctctaatta aatcgtaagt
    17401 agtattttct tctaaattcc tgtaaatgat atattcttcg cttctccacg cccaggaacc
    17461 ttctttattt tcatcttcaa ttttttcgta gcgggaagtg tttttgtcga aacataaaac
    17521 agcatattca gtgtattcgc tatgtgttcc ccacatgtaa gatgctttgt ctttttcgct
    17581 ttgacttaaa tattttacag aattaatttt ttttacccaa tcgtgtttga tttcatattt
    17641 tattgttccg ctaagctctt gagcattagt gtttaatgtg attaaaacta ttgctataat
    17701 aaagattaaa tatttcatat tattatgttt ttaaatttta aattaccacc acatttgttt
    17761 cttttttgtt tcaaaacctt taatattata tgatacactt aacatgtaat atctagctaa
    17821 agttggcgaa atactggtct caatataatt gcttccaggt gtttgactaa tcattttatt
    17881 ttgatttaat aagtcgaaaa cagctaagcg tagttcaaat ttattgtttt taccaagtat
    17941 ttgtctaatc gatgcatttg ccaaatgtaa gacttcattt aattcaaatc tgtcattttg
    18001 atattttgta aaatcataat ttgcttcaaa gaaagatttt ttagcaaatt gccatttaat
    18061 accagtgctt gcacttaaat ttataatatt ttgattttga cttgtttgaa ttgaataaga
    18121 aacatcagat aatgaacctg aagaacccaa acttaggctt aattttgaac ttaaagttaa
    18181 attaaatcct agatttaatc tgtatgaatt tgtatttgtg gtatttctta tagaattgat
    18241 ataaacaggc gattctctgt atgagtatga accaccaagc cttaaagtta atattgtttt
    18301 tacaattggc atactagacc ataaatatgc tgtaacagat tgtccaccgc taagattttc
    18361 gggtttcatt attgttgtat atcctaagcc ttcaacaaat tttgtttctt gattgtaaac
    18421 aatttggctg tcgtaatagt agtaatatac actagcatga atgtttgtaa atgaagcttg
    18481 attccaatat gtccacgata tatctgttga gtgatatgac tctggctcta agtcaggatt
    18541 tccttccgtt ttataaaatc tattacttag gtttggaatt ggttgcagat aactaatact
    18601 tggaacatct atgtcgtaag aatatcctaa gtttatatgc aaattgttgg gaaactcata
    18661 atatgtggta aaattaggaa taaagttgtt atatgtcttt gttaattctg gtactaatag
    18721 aggagaattt tcagttacag agtatttgtt aaacaaatta attgattgaa aagccccgcc
    18781 taaagaaata tttaatcctt cccaattata atttatgctt gttccaactc gattaaacat
    18841 ttctttattt tgataataaa tgctcaaact atcaaccata atgtaatttg ttaaggcatc
    18901 tgttccaaga tttttagatt gtgtgctgta gtttctaaaa ttataaaaac cgaatataga
    18961 aaatcttttc gatagtggtt caacatacaa aacgctagat tttattgtgt tatcatcatt
    19021 gtctttagag tttaaaatat ttatttgttc taattctgtt gtggctgaaa aaaactcatt
    19081 tttatttatt aaattttcca ttcttccaga ttggtagtaa ttaaaagctc cactaatagc
    19141 aaattttctg cctctctttt taaaactatg attataaata gctaaagtgt taattacttt
    19201 tgagtcaaga gtgtcggttt cgttagtaaa atttttatta atattttggt ctaaaattgt
    19261 tttaaacaac tgagttctag tatcaattga acgatttccc gaaaaccttc cataaatttt
    19321 aaaaattaaa gtattgctgg agtcaatgtt atgttcaatt cttgagctaa tgttgtgaga
    19381 atttctaaaa acatcactgg ctgaatttgt atttaataaa tatgatgtgt cagttaaaaa
    19441 agtttgccta ttagcaaaag tttctgaatt caaatctgtt tgattatagg agtaagaagc
    19501 attaaagttt attttttttg tattgtaatt ataatttacg ccacctccat aatttttagt
    19561 aaatcctttc ccatcaaaat aacccataat tcctccgcct ccaatttcca tgacatggaa
    19621 accactacca tcaccgccaa atccaaaatc accattatct ctattcgaaa aagcagcact
    19681 tcctttaaat tcagagtaat cgtcccaatt tactcctgtt tgattgacat tgttaccaaa
    19741 gccaattatt gaaagttgat gtttttcgtt aaatctatta aaattagctt ttgatgccca
    19801 aagtagtttt tttctatagt catcaaatcc acctgctaat gtagctttcc cgaaatgtcc
    19861 ttttttaaat tcgtctttta attcaagatt cattactttt tctttgttgc cgtctgcaat
    19921 tccagtaagt ctttcttgtt ctgatttgtc gtcataaact tgaactttcg aaattgcttg
    19981 agcatcaagg ttttttgtgg cactttttgg atcatcacca aaaaaagttt taccatctac
    20041 ataaacacgt ctgatatctt tgccttgaga agaaatatta ccacttacgt ccacatcaat
    20101 tcccggcaaa cgttttaaca aatcttcaac tgtagaaccc ggcggaactt taaatagacg
    20161 agcatcatat tcaacagtat cgcctttaat aaatatagga gcttgagctg atttaattac
    20221 aacttccatt aaaatttcag ctataggttc aagttttata atgcctaaat ccacagttgc
    20281 ttggttagaa acaggaatgt ttttttgcac cggcataaaa ctcacatgag aaatttttaa
    20341 aagatagcca gagtttttca cgtttttaaa actaaaatta ccctgagaat cagaagttgt
    20401 gtaatttatt aaagtggaat ctttgctgtt tagtaacatt acagtagcag caggaacaat
    20461 ttcatcattc tcatctatta tctgtgcttt gatagtaatt tgggaaacct gagccttgcc
    20521 tattaagcta aaacttacaa gcactaaagc gagaaaaaca ctttttatta aatgtctcat
    20581 tattaatgtg tttatgtgta ataataaaac tgcaagattg gtaaaatatt ttaataaaca
    20641 ctttaaaata atgttaaata ttttaataag ctgtaaaaat aaattttaac taaaaaaaca
    20701 attttataaa ctcgtttagc caccttaaat cgtaaatccc aaaatcctca aatccaaaat
    20761 cccaaatccg aaatcataaa tcaccaaatc cccaatcggt tgtagagcaa aattaaagtt
    20821 tatatgtaaa attgttataa attagaacaa atgtttgttt agtattagaa catttgttat
    20881 ctttgcagtg tgaaaaacca aaaaaataaa atatgaaaac aaatttatcg caaaagcaaa
    20941 tcggtcagcg aataagtgaa ctacgaaaaa caagaggatt tactcaagaa gaattggcta
    21001 atagacttga aatttcccgt tcatcattag ctcaaattga gttgggaaat agaaatgttg
    21061 atattcttga attacaaaag ctatctatga ttttaaaatt ttcacttgat gattttatgt
    21121 ctaagaattt ttctgctatt aaaaatattg atgtgaaaga agaaaaagaa gaaaaaaaat
    21181 caggagaaag aatttctgta cctaaattac aagttaacaa atttaaaaac atattgcttt
    21241 atatacttga acgttgtgct ggcaaaccaa atgttggaga aacagttctt tataaattgc
    21301 tttatttctc tgattttaat tattatgagc tttacgaaga gcatttaaca ggtgccaaat
    21361 atcgcaaatt accttatgga cctgttccac aaaaattaga tgcgattatt aaccaaatgc
    21421 ttgagaaagg acaattacaa aaagtaaaaa cagaatatca tggctatcct caaactcgat
    21481 atttaccttt agtaaaagct gatttaacta aactaaaagc aagtgaaaag gaagttattg
    21541 acggagttat agaacaaatg agcgactggt cggcaactat gattagcgaa tattctcaca
    21601 aagacttgcc ttgggaagtt acagaagaag gagattatat aagttatgaa ttggcttttt
    21661 atagagagtt accttattcc gtaagaattt acgaagacga aaatgattaa actatggatt
    21721 tcaatcagct acctgaattt gaaaaagatt taaaattctt gttaaaaaaa tatagaactt
    21781 taaatgaaga cctaaatgaa ataaaaacga ttttaagaaa gaaaccgctt gaacgtcaac
    21841 catttagttt tcgtatcgat aacctaagtt tggaaacacg cattattaaa gttagaaaaa
    21901 ttgcttgtaa atcattaaaa ggtaaaggag taaactcagg tttaagatta atttatgctt
    21961 actttccaaa cgaagaaaaa attactttta ttgaattata tcataagaac tacaaggaaa
    22021 atgaagacag acaaagaatt taaataattt taaataaaag atgattttca atttttccaa
    22081 aaactctatt ctgtgataaa acaaattgca cattatctct gtaaaaaagg aatttatgta
    22141 acatatttta gaacaaaaac acctaaatca ttattgtttt acggacaaac ctttggcagt
    22201 cgtccttctg acatagattt attcaaaatc aaacttttaa ctgaaacaat tctcaatttt
    22261 ctaaggagca agtgcagagc caaacttgtt tgaactctgc cgagagacga tagaaaatgt
    22321 agcgaagcta aattattcaa ttctcaattc ttaattagtt taatccaaaa tccatttaaa
    22381 attaaaaact caccactaaa aactcaccac taaaaactaa aaactcttta aatccaaaat
    22441 ccaaaatcca aaatcccaaa tccgctttcg acttttaaca cttcaactac gttcagtgca
    22501 tcgcttttaa cttttaactg aattaattta tctttgtggt ctaaaatttt attaaatgca
    22561 aatcttagaa gcacttaaaa aagagataca aggcgaagtt caagttgata atatttacat
    22621 aactctttat gctacagatg cctctgctta tcgcgaaatt cctaaagctg ttgtttttcc
    22681 taagaataaa gacgatttaa ttgctattgt aaaatttgcg aataagcaca atttaagttt
    22741 aattccaaga gcagcaggca catctttggc tggtcaggtt gttgggaatg gaattgtggt
    22801 tgatacttcg cgggctttta ataaaatttt agaaattaat aaacccgaaa aatgggtgaa
    22861 acttgagcca agtgtggttt tagatgagct aaataaagtt ttagcaaaag aaaatctatt
    22921 tttcggacca gaaacttcga cttctaatcg ctgtatgatt ggtggaatga ttggaaataa
    22981 ttcctgcggc gcacattctt taatttacgg ttctactcgc gaccatgttt tggaaatgga
    23041 ctgtattttg agtgatggct ctgaagctca ttttggagaa atttctaaag ccgaatttga
    23101 agaaaaatgt aatttgcaaa atctcgaagg cgaaatttat agacaattaa aaactttgct
    23161 aacagaccca attattcttg aagaaatttc aaagcaatat ccagacaaag aaattaaaag
    23221 acgaaataca ggctatgctt tggatttgct tgcagacagc gaaatttttg gtaattcaaa
    23281 cgagaaattt aatatgtgta aacttttagc aggttctgaa ggaactttag cttttacaag
    23341 ctctgtaaaa ctaaatttgg ttgatttgcc acccaaaacc aaagctttaa tgtgtgtgca
    23401 tacaaaaagt ttacacgaag tctttgaagc aaatcttgtc gcattaaaac actctccagc
    23461 ttctatagaa ttgatggaca aaaaagtgtt ggatttatca aaagcaaata ttaagcaaaa
    23521 tagaaacaga ttttttataa atggcgaccc agctgcaatt cttatagtag aatggtttgc
    23581 cgatgatatt gaaacaataa ataagcaagc agacgaatta gaaaaagaat tgcgttcaaa
    23641 atctttgggc tatgattatg caagggttta tggtggcgat attgcaaggg tttgggaatt
    23701 gcgaaaagca ggattaggtg ttttgtcaaa tatgcccggc gatgctaaac ctgttccagt
    23761 tattgaagat accgcggtta gaccagaaga tttatctgca tttataaaag aatttgagga
    23821 gttaatgcaa aaacttggct tagattgcgt ttattacgct cacattgcaa caggtgaact
    23881 gcatttgcgt ccagttttaa atttgaaaga caaaaaacat gtagaacttt tcagaaccgt
    23941 agctttagaa accgccaaat tggtaaaaaa atataaaggc tctttgagtg gcgaacatgg
    24001 agacggacgt ttgcgtggcg agtttattcc tttgatgatg ggagaaaagt tgtatgatgt
    24061 ttttaaggaa ataaaaaatg tttgggatcc aaaaaatatt tttaatccaa ataaaattgt
    24121 ggatactcca ccaatgaata cttcgttgag atataaaccg ggtgttgaaa caaaagaaat
    24181 aaaaacaata tttgattttt cttcgacagg tggctatatg cgcgctgtag aaaaatgtaa
    24241 cggttctggc gattgtcgca aatctcatat tattggagga actttatgtc caagttttca
    24301 ggcaactaaa gatgagaaaa actctacacg agcacgtgca aatatgttga gagaagttct
    24361 tacaaattca actaacaata agccctttaa ctcaaaagaa ttatacgaag ttttagactt
    24421 gtgcctgctt tgcaaggctt gtaagtctga atgtccttca ggtgtagatg tggcaaaatt
    24481 gaaaatggag tttttgcaac attattatga tacaaatgga gttccgttgc gttcgtttgc
    24541 agttgcttac ttgccagttg tttataaaat atttgcctct ttgccgggtt ttgtaaatat
    24601 ttttctaaaa aataagtttt tctcaaaaat tttaaaaagt ataattggtt ttaatcaaaa
    24661 aattgaattt ccattgatgc ataaaactgg atttaagaaa ttgtacaagg gaaaacaaaa
    24721 atttgaaaat ccaaatccta taaaaactat ttatttgttt aatgacgaat ttacaaaata
    24781 tttagaaagt gatttaggaa taaaaacaat tttatttttt gaaaaactcg gctatcaagt
    24841 aaaacttgca ccaataaatt ttagcggaag aacttggttg agcaaaggtt tggtaaggcg
    24901 tgcaaaaaaa cttgcaaata aaaatatcga aattttaaaa aatattattt cgtctgagac
    24961 acctttgatt ggaatagaac cttcggcaat attgagcttt agagacgaat atcctgattt
    25021 agttaatgct gaaaataaaa atgctgctca ggatattgca caaaactcat ttttatttga
    25081 tgaatttatt gctgctgagt ttgaaaaagg gaatatttct gctaattctt ttacagatgc
    25141 agagttgaaa attaaatttc atgggcattg tcagcaaaaa tcattgatta caaccaaatt
    25201 taccaagcaa atattatcaa ttcctaaaaa ttataaagtt gccgaaatcc caagtggttg
    25261 ttgtggtatg gcaggttcgt ttggttacga aaaagaacat tatgatttat cgcaaaaaat
    25321 aggagagttg gtattattcc cagaagttag aaatgcagat gatgatacag ttattgtagc
    25381 tgttggaaca agttgccgtt gtcagataga aagtggaact ggcaaaaaat ctttacatcc
    25441 tatcgaagtt ttgtgggaag ccttagttta aatcttttat ttcaaagctt ttctaagttt
    25501 cacaagtttt tctaataagt cgtcaactaa atctaatttt agcatatttg caccatctga
    25561 tagtgctttt ttgggttcgg ggtgagtttc tataaaaagt ccgtcagcac caacagcaag
    25621 accagctttt gccatgtact caatcatttc tggctgaccg ccagtaacgc cacttgattg
    25681 gtttggttgt tgtaaactgt gagtaacatc aagcacaaca ggaaagccaa aagttttcat
    25741 tttaggaatt cctctaaaat caattactaa atcattatag ccaaaggtag taccgcgttc
    25801 tgttagcata atgtttttat ttcctgtaga aagaattttt tctgtggcaa atttcattga
    25861 ttcgggagac aaaaattgtc ctttttttat gttcacaaat tttccagttt ttccagcagc
    25921 aataagcaag tctgtttggc gacataaaaa agcaggaact tgtacaatat cagcaacttt
    25981 tgcagcaatt tcggcttctt cgggcgagtg aatatctgta caaatatgta gttttaattc
    26041 ttgttttact tttgataaaa tttctaaagc tttttcatcg cctaaacctg tgaatgaatt
    26101 tacactactg cggttagctt tgcggtacga agatttaaaa attaactgaa tattgtgttt
    26161 tttgcataaa tcttttagaa cttttgcagt atgtaatgtg atttcttcat tttctactac
    26221 gcaaggtcca gcaattaaaa aaaaatgtgc agaattgtct aaaattaaat tttccacttt
    26281 caaaatattt atatttctac aaaaatataa aattaaatca ctttaaaaac ctaatattta
    26341 attaattttg tagcaaaaaa caatgtatag aatttcttta gtttcttact caaatacttt
    26401 gccttttaaa gaggctttgt ataattctga gtttgtagct aaaaatgcaa ttttacaaga
    26461 acgtattcct gctatgtgtg ccgaagatat ttttacagga gaagcagata tttcattagt
    26521 gccaatagga gcatttccta ttcccgaaaa cacaagaatt attagcgatt tttgtttggc
    26581 agcaaaaaat aaagttgagt cagtactatt ggtatcgcaa aaaactaaag aagaaattaa
    26641 aaccataatt ttagattatc attcacgaag ttcggtaaaa tatattaaga ttttggcaga
    26701 aaaattttgg aaaataaaat ataattatca aaatgctgac ggaaatttta aagatttaat
    26761 aaaagaagat actgctgcag ttattattgg agatagagct ttaaatttcc gtaattctat
    26821 aaaatacaaa tatgatattg ccgaagaatg gtttaaattt acaggtaaac cagcgatttt
    26881 tgccgtttgg gttgcaaaca ataaggtgag tgatagtttt ttaaaagagt ttaacaaaat
    26941 tttgaaatct ggatttgaaa atcgaaagca aattgctgaa aaaaataaac atttatatcc
    27001 caatttcgac ttagttaatt atttaacaaa ctgcatggat tataattttg atgaagctaa
    27061 acaagaaagt ttaaacatgt ttaatcaatt ttattctgaa attttaagat aaaaactata
    27121 tttgtaaatt ttgtgaaaaa gcgatttaaa ttaaagaaat atgaatttac aaattaaaga
    27181 ttttgaacca aagcactacg acgaaatgaa tgtcctgtgg cagttgaccg acatgggcgg
    27241 agcttatcgt ggagataatt tggaaataat tcttaattca ataaaaatgg gcggtgcctt
    27301 gtatcttctt tttaatgacg atgttatgat tggcacttca tggattacaa atgatggtag
    27361 aaggctttat ttgcatcatt tttgtattca tcccgaacta caaggcaaag gattaagcaa
    27421 attattaatg gacaaatcta tggaatttgc cagaaaagta ggtttgcaaa taaaattaga
    27481 agttcataga gaacatgttg tagcccgcaa tctatatgat aaatatggct ttaaatattt
    27541 aggagattac gatattttta ttgttagaga atattaatgc cttgagaaaa caaattttaa
    27601 atttagctta ccctgcaatt atcagcaata ttaccgtacc tttagtaggt ttggttgata
    27661 tagctttagt tggtcgttta ggctcggcgg cttatattgg cggtgttggt tttgcaagcg
    27721 tagttttttc ttttatatat tcgggcttag gttttttaag aatgagtaca aatggactca
    27781 cagcacaagc ctatggagct tcgaatttta gagaggcaaa ttcaatttta attagagcaa
    27841 ttattcttgg cgttcttagt ggattgttgt taatagcctt gcaaacaccg ttagaaaggc
    27901 ttttaataag tatgtttgat tcttcgccag aatctttaaa atatgcttct gaatattttt
    27961 atgtaagaat ttatgcaaca ccagcaagcc tggcagttat tggttttatg ggttggtttg
    28021 tgggaatgca agacactaaa acgcctatgt gggtaacctt gataataagt ctcgcaaata
    28081 ttgcttttag tcttttcttc gtaatttatt ttaaaatggg agtaaaagga gtggcacttg
    28141 gaactgttgt tgcacaatat ttaggactga tttcttcaat tttattttat gtaaaaaaat
    28201 acaaatctta tttgtcttat atagataaaa aatccatttt aaaatttgat gaaataaaga
    28261 agttctttaa tattagtaaa gatatttttg tgcgttctat cttactcaca ggaagttttt
    28321 tcttatttaa tattgtttct gccgattttg gcgacgaaat cttagcctta aactctgtga
    28381 tgttacagtt tttatggttt ttctcatatt ttgccgatgg ttttgcaaat gcaggcggta
    28441 gcattgccgg aaaatataaa ggagcagcaa atccaaaact tttaaaagaa acaataaaag
    28501 aatcgttgag attaggactg ttggtagcta ttattttttc tgttgtttat gtgttctttt
    28561 ccaaacaaat tgtgcaaatc ttaaccgatg atgttcaagt aattagtctt tttgaaactt
    28621 atcatttttg ggtttggtta atcccaatta caggttttgc agcctttatt tatgatggaa
    28681 tttacatcgg aattacagag tcgaaaataa tgagaaatgt tatgattatt tcaattttga
    28741 taatatatgt tccactttta tttttagcaa aacattatat tggaaatcat ggaatgtggg
    28801 cagtgctaat tttaatgctt ttaacacgag gaattggact gaaagtccag ttaaaagtta
    28861 aaagttgaaa gaggctgcgc gtaatcagtt aaaagttgga aagggtgtaa gattatggtt
    28921 atagaaaaga aaaaaatagc aatacaagat acttatggtg aaagatttca gcattgttgg
    28981 ggttgtggac caaaaaatga agagggttta catttaaaga gctatccgaa tgaagatgga
    29041 agccaatgta tttgtaggtt tacgccagca gataaatata caggtggggt tccagacaat
    29101 ctttttggag gcatgatagc ggttattttt gattgtcatg gaactgcttc tgctgcttgg
    29161 tttgctcata ataaaaaagg acttgaactt acagaaaata ctgtaatagg gcgttttgtt
    29221 acagcaagat tagaaattga ttttaaaaaa ccacttccta tgaatgaaga aataacagtt
    29281 acctcaatac ttgaagaaat tggagaaaga aaagctatag ttaatatgaa aatggaagcc
    29341 ggcggactcg tttgtgctaa agcaaagatg attgctgtta gtttgtaaag ttcgtaaagt
    29401 tcataaagtt tataaagaag gcaagtattt gattgtcaat agtttgtccg tagggcaaca
    29461 caatgaaatc agttaaaagt tttcaaagtt cataaagttt ataaagaagg caagtatttg
    29521 attgtcaata gtttgtccgt agggcaacac aatgaaatca gttaaaagtt tgtaaagtgg
    29581 agttagtttg tgggtttcgt caaagtttaa agaattaatt atatccattt ttaactcatt
    29641 tttcaacttt taagaaaaat tagtcataaa atattttgtt caaaccctta gctttttacc
    29701 aagtcataat atcctcaaat ctcttaaact tcacacttta caaactttac aaactctaca
    29761 aacactttat taaacctgaa agcatttttg aaacttcttc tgacattgca aaaagtaatt
    29821 taaaatcatc atcagatatt ttgtttaaat cttttgctaa atatagcata gagcgaattt
    29881 cgccacaaga acctttggct ataaataaaa aatgcttaaa ttcgttattg cttttacgtt
    29941 caaacccttc agcaatatta ttcattacag aaatagaagc actttgtatt tagtttttaa
    30001 attcaaaatc tttactttct ttaaataatg aatatactct aactgttaaa tctttagctt
    30061 tttgccaagc cataatttcc tcaaatctgt taaacttcat actttaaaaa ctttatatac
    30121 ttttaacttt acgaacttta caaactttac gaactttatg aactttcaac ttattttgag
    30181 caacctatgt tttcaaactt ttcctgccac ccttcctcag catagctaat tatttcagga
    30241 cattcagtgc cgaaggagta aagctttccg tattttatat cttctataat taattttttg
    30301 ataggattaa tttcgctatc gccaagtgat ttgtgaacag catgaatatc tttgcaaaac
    30361 aattttcttc ctttaaaata tccgtttcct acattgtaaa tatatgcgta attacatctg
    30421 ccttcaagtt tgtagtcgcc tgtggaggca tgacttctaa aatacaaact gtcgccactc
    30481 aaaatcatgt ctgtagttaa aattcctgcc cagttttgta tcgagagagt attaaaatat
    30541 aaactatcta aagtttttaa atcgcaagtt tcgtttattg taatttcttt taagttagga
    30601 caagtaatgc ttaattttgg tttaattctt tttcttagcc attcgcaagt gtttctattt
    30661 tctaaaatca attccttgcc ttcaatttcg tgactaataa atggaattaa attttcgcca
    30721 caattaatac ttaaaatatg ttcgctacca gtgataaatg taatttcaaa atcgctttta
    30781 atttgcactt tattaaaatc atctaaatag attttttcag ttgtaatttc gccagtagat
    30841 ttcatacagc ggtttttatc ggtgtcgcaa gcattaaagc ctaaactaaa aaccacaatt
    30901 attaaaaata aatatctata ttttttcatg tattaaactt aaaaaattca atcgctaaga
    30961 taatattttt taaattaact ttatattgtt aaaaaaacaa aataagtcaa tcgtttattt
    31021 cgttaaatct tagcatattg caactaaaaa tattgaatta tgtggagaat gtcttttgta
    31081 atttttttaa cttctgtact ttgcttggtt accacttctt ttaactataa aaccaacgat
    31141 tttccaacaa agagaacaac aaaaccacct tcgtttttag acgaaaataa tccttgggtt
    31201 gattcagttt ttaatacttt aacacaagat gagaaaatcg cacagctatt aatgctgcct
    31261 gcatattcca ataaaggcaa gtctcacgaa gaacatattt taagtttaat aaaaaattat
    31321 aaaatcggcg gtttaatttt tatgcaaggc ggtccgcata ggcaagctgt tttgttgaat
    31381 aaatatcagt caaattctga gttgccactt ttaatttcaa tggatgcgga gtggtctttg
    31441 agcatgagat tggactctac cgtgctttat ccacggcaaa tgatgttggg agcaattcaa
    31501 gataatgagc ttatttatga aatgggagct gaatttgctc gacagttgaa aagagtagga
    31561 gttcatgtta gcttttcgcc tgtttgcgat gtaaacaaca atccacttaa tcctgtaatt
    31621 aacgacagat cttttggcga agataagtat aatgttagca taaaatcata ttattatatg
    31681 aaaggcttgc aagacaatgg agttttggct tgtgctaaac attttcccgg gcatggcgac
    31741 actcatgttg attctcacaa agctctgcca gtgattaatc acagttttca gcgtttagat
    31801 actttggagt tgtacccatt tcaatacttg attgataaag gaattgcttc tgttatggtg
    31861 tcgcatttat ttgttcctgc aattgattct tccaaaaatt tgccaagttc tttgtcgccc
    31921 aaaacaataa acgaactttt aaaaaccaat atgggattta aaggtttagt atttactgat
    31981 gctcttaata tgggcggaat taccggaggt tttaaagctg gagaagcaga tgtaaaagca
    32041 cttttagcag gaaatgatat tttacttttt ccaaacgaag ttcctttggt tattgaaaaa
    32101 attaaacaag caatagaaaa tggcgaaatt tctcaagaag aaatcgataa tagatgcaaa
    32161 aaagttttaa tggctaaata ttgggttggc ttgaataaaa ttgaagaaat ttctttagaa
    32221 aatttgcatt cagatttgaa caatacagag gctttgttga tgaaacaaaa attgatagaa
    32281 aattccttaa ctttagttac aaataaaaat tccttaattc ctttacaaag attagacaca
    32341 ttaaaaattg caagcataag ttttggttct aataatatga gtagctttca aaatcgctta
    32401 aaatattatg ccgaagttga tgagtttaat tttgaaactg aaatcaaaaa aagtggtagt
    32461 cagggtttta aggcaaaact taaaaagtat aatttgttaa ttgtgggcat tcacaacaca
    32521 aattctttag catctcgaaa gtttggaatt tcaaagcaaa caactgaatt tgttgatgaa
    32581 atttgtgcag aaaacaatgt gattttagac ttgtttgcta atccttatgc tttggcttat
    32641 tttaaaaatg ctgaaaaatg tgctgcaatt attgtttcat acaacgattg gacattgaca
    32701 aatgatttgt cggctcagtt aatttttggt ggaattccag ctttaggacg tttgcctgtg
    32761 agtgtaggaa ataaattttc agtaaattcg ggttacgata ctaagaaaat tagattgaaa
    32821 tacacttcca ttcccgaaga agcaggagta gattcagaaa atctttacaa agttgattct
    32881 atgatgaacg aagcaattaa cgccaaagct tttccgggcg gacaaattgt ggcagcaaga
    32941 aatggaatag ttttttatat aaaatccttc ggctatcacg attattcaaa caaaattaaa
    33001 gtaaatgatt ttgacttata cgatttggct tcggtaacta aagttgtggg gtctgcacca
    33061 gcacttatga aactttatga cgaaaagcta tttgctttga acgataaatt atctaaacat
    33121 attccaattt tgcaaaatac taaaaaagcc gattttgaaa tttttgatat tttaacccat
    33181 agagcaggat tcaaatcttg ggaagctttt tttaaaaaaa ctattaaaaa cgatagtgtt
    33241 caaaatttaa tttattcaaa agaaaaagca gaaaattaca atacaagagt ttgcgaaggt
    33301 ttttatattt tagattcgta tcgcgacaca atttttcaag acataattaa ttcgcctcat
    33361 aatagatacg gaagatatgt ttattcagat atgggatttt attttttccc agaaatgatt
    33421 aaaaaattta aaggaaaatc tatggacgag tatttgtacg acgagtttta ttctcgtttg
    33481 ggggcatgga ctttgcgtta ttcgccatta gaaaaatttt caaagaaaaa tattacgcca
    33541 accgaagaag ataatgtttt tagaaaagaa caattacacg gctatgttca cgaccaagga
    33601 gctgctatgt tgggcggaat ttcaggacat gcaggtttgt tcgcttcggc taacgatgtg
    33661 gcaaaagtta tggaaatgtt tatcgaaaaa ggaagctatg gaaatttcga ttatataaat
    33721 tctgaaacta tagatttatt tacaaaatat caattcgatt cacttataaa cagaagaggc
    33781 ttgatttggg ataaacctgt tccaaacgat acaacaaaag gattgggtac tggttcttca
    33841 tcaaatttag cttacggaca tagcggatat acaggcacta tggtttgggc agacccacat
    33901 tatgatttta tttatgtttt caattcaaac cgtgtgtata aagatgctga aaactggaaa
    33961 attttaaaac taaacactcg aactattata gaagaaattt tttatcaagc aataatcaag
    34021 aaaaatccag atttgaaata tgtaaaaaac taaattctct gctaaaaaaa tcagaaaatt
    34081 aattattttt gtaaaaattt taaaatgact gatagttcca gatataattt gcgtggcgtt
    34141 tcagcctcta aagaagatgt tcacaatgca attcgtaaaa tagataaggg attatatcca
    34201 aaggcatttt gtaaaataat tccagatatt ttggctggcg atgaaaacta ttgcaatata
    34261 atgcatgccg atggagcagg aacaaaatct tctttagctt atatgtattg gaaagaaaca
    34321 ggcgatttgt cggtttggaa aggtattgca attgatgcaa tagttatgaa tattgatgat
    34381 ttgctttgcg ttggtgctac cgacaatatt ttgctttctt caactatagg acgaaataaa
    34441 aatttaattc ccggcgaagt tatttctgca attataaatg gaactcaaga gttttgcgac
    34501 gaaatgggca agcatggcat taatattgtt cttacaggtg gcgaaactgc tgatgttggc
    34561 gatttggtta gaacaataat tgttgattca actgttactt gccgaatgtt gagaaaagat
    34621 gtaattacaa acgaaaatat tcaagcaggc gatgtgattg tagggctttc ttcaactggt
    34681 cgtgcaagct acgaaaattt ttacaatgca ggaatgggaa gcaatggatt aacttcggct
    34741 cgacatgatg tgcttgcaaa ttatcttgcc gaaaaatatc ccgaaagcta cgataatgca
    34801 atgccagcag aattagttta ttcaggaaag aaaaaattaa cagaaagttt agaaaagcct
    34861 tattcagatg ttggaaaaat gattctttcg cctactcgaa cctatgcacc agttattaaa
    34921 gaagttttaa agcattattc taatcaaatt cacggaatta ttcactgtag tggcggtgct
    34981 caaacaaaaa ttttaaattt cgtagataat cttcatatta ttaaagataa tatgtttgaa
    35041 attcctttgt tgttccaaat gatacaagag caaagcaata cagattggaa agaaatgtat
    35101 aaagtcttta atatgggaca tagaatggaa ttgtatttgc ccgaaaacat tgctaatcag
    35161 gtagttgaga ttagcaaaag ctttaatgtt gacgcaaaaa taattggtag ggtagaaaag
    35221 agcgaaagca aaaaacttac aataaaatcc gaaaagggcg aatttatata ttaagagaca
    35281 gattatggca aacgatttaa ttttagataa acttagagat gtaaaaaaac gatttttaga
    35341 agttggaaat ctattaacag atgcaactat agtgtctgat atggataaat acatcaagct
    35401 aaataaggaa tataaagacc ttcagcctat tattgaggct tatgatgaat atgttttaat
    35461 tatttcaaat attgaagatg caaaaaacat tttagctaat gaaaaagact cagaattgcg
    35521 tgaaatggca aagatggaat tagatgattt gcaaccaaaa ttagagcctt tagaagaaaa
    35581 aattaaatta ttattaattc ctaaagaccc tgaagatgat aaaaatgcca ttattgaaat
    35641 tcgtgcaggt acaggaggcg atgaggcaag tttatttgca ggcgagctta tgcgtatgta
    35701 tgtaaaattt gcagaatcga aaaattggaa aaccgaaatc acctcataca ccgaaggaac
    35761 ggttggtggt tataaggaag tagttttaag catgacaggc aatggtgttt atggaattat
    35821 gaaatatgaa tcaggagtgc atcgtgtgca acgtgttcca caaaccgaaa ctcaaggacg
    35881 tgttcatact tctgctgctt ctgtagctgt tttgcccgaa gccgatgagg ttgatataaa
    35941 attaaatcct gccgacatta gacgcgatga gttttgttcc tcaggaccag gcgggcagtc
    36001 ggtaaataca acatattctg caattcgttt aacgcatatt ccatcaggaa tagttgttac
    36061 ttgtcaggac gaaaaatcta agcttaaaaa tttagatgtt gcaatgaaag agcttagaac
    36121 tcgactttat aatttggaac atcaaaaata tcttgatgaa attgccaaaa agcgtaaaac
    36181 tatggtatca acaggcgacc gctcagcaaa aattagaacc tacaacttcc cacaaggacg
    36241 cattaccgac catagaatta attatactat gtataatttg caaaacttta tgcttggcga
    36301 aattggcgaa ctaattgacc acctaattat ggctgaaaat gccgaaaggc tgaaagaagc
    36361 taatgaggag taggggggca atggtttggg ggtatgaatt agtgcgagat tttataactt
    36421 ttgcctgtcc cgcggctatg tatagtgaat gtactcatgt tcaattgacc acattgtcct
    36481 gcattactta tgaccgcgtg ttatgtggag tatttattat tcatctctat aatcatcaaa
    36541 tctaaaaagg tctgaaatat ttcttaaatc ttgcttgctg ttagtaactc caccaacgga
    36601 aactgtcaga actctcgaaa gggtagtgtc cttcatctcc aaaaatgccg actacacctt
    36661 tggattcttt atgttgattc gttaatcttt ttgatagatt ctttttagcc atatttttat
    36721 tccactatct tttttttgca aaaatacaaa ctttattcta tcgctaaaaa atacaatgca
    36781 atatctccaa gtttgcagtg ctgtttgttt ttgcttgcag gtaacgtttt gcggctttgc
    36841 gttcgggcgg gtttcggagc acaaaactgt caaccagcac tgcacttgaa tagaagcaca
    36901 aagctccaag tttgcacgtc accccgcctg acgcaaaacc cgtgttggca gtagtgcttt
    36961 tagttcctct ttgcagcatc tcttatggct tgctccaaat tgtctccctg caattcatat
    37021 ttttccaatt gagctacatt atcttgtctg atgtgtagtt gtccttcatt gcccttatta
    37081 cctctgtgtt gaccttttga gtaagatgga ttttccaaga cttctttact gctcataacc
    37141 caaaggacaa tttggtctct gtaaacagca acccaaataa atacgtcaca gcattgtggt
    37201 tttagctgct gaaaattcat caagaagttt ttgtcagttt ttcttgaaag agctttcata
    37261 tacagaggtt cttcactgtc actatcaact gcccttgatg ctttaacctc aattcttatt
    37321 ccgtcaagcc ataaatcata gtcccctgag taattcgggt caagtttttt attgggactt
    37381 tttagcttag aacacttgcc ctgaacataa gtctgtgcaa acttttcacc ggatcccctt
    37441 ggtgcagata tttcaaaaac ccaaaggttt ggatttgcac taatatattc agccctaatg
    37501 tctatgtatt gttcatatgt caagccaccc cgttcaatta aattgcaaat caggtgttcg
    37561 tattcattaa atggaaaatt gactggtcat catcaaccat ttgaagaacc tcttcaaggt
    37621 cgcttgctga accagaaaaa gattcaataa ttttagtctt taactcggta agttccatcg
    37681 cttgttttga ttatttgtaa atagtttaag taatcggttt ctgtaaattc ctgattgaat
    37741 gaaaagtatt cgtggtgtat tggaagtaat tcaaggtcac ttctaagaac cttatgggtt
    37801 gaaaataatt ttttgaaaag ccagttaata atttcgctat ttaatagcat agttagttgt
    37861 tcggcagata ttcctatatt caaaggaata agaagattag cactatttaa gatataccgt
    37921 tgttttgtat cacaatagaa acacaagtct gatgagataa acttgtaaat tagtttttcc
    37981 tttgaatgat acatttctaa gggcgcgacc tgctgaaagt ttgaaaaatc actaaaaata
    38041 aaagtgcttg gttctttaag tcctaatttt gttatatccg aacctttgta aataggaaca
    38101 taatcatctt gcggttcatt actgcaaaat ctatcattat tccccgtcac tatacctaat
    38161 gcccaattag ccctgtcttt gagtgttatg tgttttattg aataaagctg tttaataact
    38221 tttgcttcgg tctcatttgt ccaaaaatta aaaatgtgtt taggattttt tttaaaggaa
    38281 tccaatgttc tattgaatac attattttct gaacaacatt ttataatgtc gtttgggtca
    38341 gaagcattgt tttcaataac aatggcctgt gctttggtaa ctagaccttt gaatgcttta
    38401 ccataatcca caaaccgtag tatttttttt gccaatactc tttttcggat gtcttcaaaa
    38461 gtagtaatgt tgaaaaatgc ctcttggatt aaaaaaccta agaatccgtt aactttcaaa
    38521 atagataagc tcgctgccaa aaataaagac gttgtgtcta aactatttcc gcaactatac
    38581 aatgcggaat acttctcttt tgcagatttt tcaattttct taccccaagg cgggttagtg
    38641 aaaatgaggt cgtaagtgat tttttctgtc tttaatttgt gtgcttcatg taaaaaatca
    38701 ccaactttaa tgttgtgggt atcaatacca aactcctcct ttattctctg tttagcaatc
    38761 agcactgcat tttcatctac gtcaaacccg taaacgtttt caggtgctac tccctgccta
    38821 attgcttcaa taagaaaatt accagacccg caacaagggt ctaaaaacgt agaatcaggg
    38881 ttgattttta tgttcttgaa catatctttt acaacccaag aaggagtgta ataaataccc
    38941 tccttgtttc tatacgaatc tgcaagtaaa ctttcgtatt caatgcctat ctgttctcca
    39001 ttgaattttt caatcagcaa actcaatttg tcagaaactt cttgatggtc gtggtcatct
    39061 tttaataatt tgtttgctct tgcatttagt ttttcttttc ctgcaacctc agacataaat
    39121 ttctcaaaag actcctgaga aacaattcct ttacctgatt gaaataagta acctgtctta
    39181 atccaattac gaatggttgc agttgaaaca ttagcggttt ttgccgcctg ttcaattgtc
    39241 agtccgtatg tttctatttc accaaaaata tttgtctgca taactcaaat gtcgttaaaa
    39301 ttgtcgcaat attgggagat gtctgatttt ttagcattac tgccaacgta tattttacga
    39361 aacatctatc caacattcgc atattatttt ctataaatca attatattca tttacaaaac
    39421 taattaaaaa tttttt
//
LOCUS       DAOPNX010000015        38205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_1439_length_38205_cov_7.216619, whole genome shotgun sequence.
ACCESSION   DAOPNX010000015 DAOPNX010000000
VERSION     DAOPNX010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 38205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38205
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1439_length_38205_cov_7.216619"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..923
                     /gene="rhuM"
                     /locus_tag="PKN53_02895"
     CDS             57..923
                     /gene="rhuM"
                     /locus_tag="PKN53_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HOD87932.1"
                     /translation="MSEIVLYQSNELAKHVEVIIDEKTETIWLTQQQMADLFGVQRPA
                     ITKHLGNIFKSGELEENVVSSILEHTTQHGAIEGKKQKTSVKYYNIDAVLSVGYRVNS
                     ARATQFRIWATRILKDYLLKGYALNTRINRLENNFENLKNKVNEIDLQINTHLIPTQG
                     VFFDGQVFDAYELASKIIRSAKESIVLIDNYIDETTLTHLSKKAKTIKVLLLTKTISK
                     QLTLDVKKANEQYKNFEIKTFAKSHDRFLIIDNTEVYHLGASLKDLGKKWFAFSKMDK
                     NSVETILKEIEK"
     gene            920..1951
                     /locus_tag="PKN53_02900"
     CDS             920..1951
                     /locus_tag="PKN53_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006282999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOD87933.1"
                     /translation="MKEIEKQPPELYSDVCRIIENTRTRLATTANAEVCLMNWQVGLR
                     IKTEILKDQRAEYGKEVIKNLAKLLTEKYGKGWSLYKLQHCVRSAYVFAEDEIMYAVR
                     TQLTWTHLRSLMSINDELKRRFYMEMVRIEHWDTRTLDEKIDGMFYERTILSRKPEEF
                     IKQELQQIQTTHTLTPDVVFRSSYFLDTLGLADTYSEKDLEDAILINLQSFIKEMGSD
                     FAFLDRQKRITIDAVDYRIDLLFFHRGLKRLVAIDLKLGKFKPEHEGQMLLYLRYLNK
                     NERKEGEGSPIGLILCSEGNTEHIEYLMLEESNIKVAQYYTQLPDKKILSEKLHKAIA
                     IAREAKQSK"
     gene            1951..3165
                     /locus_tag="PKN53_02905"
     CDS             1951..3165
                     /locus_tag="PKN53_02905"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOD87934.1"
                     /translation="MSKTKKIIPEFRFPEFKGEWSLEELGQCLDYLQPTPFLVDNTDY
                     NDEYEIPVLTAGKSFILGYTNEQEGIFNENLPVIIFDDFTTASQFVDFPFKTKSSAMK
                     ILLAKNDNDIKFVYESMQTLNYEVGVHKRHWISVFSKLRIAVPKPQEQQKIASCLSSL
                     DELIAAHKDKLDALKDHKKGLLQNLFPQDGEKVPKLRFKEFENSGEWEEKKISDLGET
                     ISGLTGKSKDDFGAGKPFVTYKQVFDNSEINFEQCERVQISDDENQTSLQQGDILFTT
                     SSETPNEVGFASVLIDFPKEKTYLNSFCFALRPFNIEKTQPQFSRYLFHSPIYRRSVT
                     AIAQGSTRYNISKGAFVEIQVPIPAPTEQQKIASCLSSLDEVIEAQVAKIEQLKLHKK
                     GLMQGLFPKIND"
     gene            3169..3765
                     /locus_tag="PKN53_02910"
     CDS             3169..3765
                     /locus_tag="PKN53_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOD87935.1"
                     /translation="MEKFQNKYRVSSFRLKNWNYCSEGDYFITICTQNMEHYFGKIEN
                     AKMQLSPVGVIADILWYQIPYHSKNVELGAFVVMPNHIHGILTIVKNNNKNCLDNPNI
                     PTQPTIPLSSALPISSMSYSKKNERMAKISPKKNSISSIIRSYKSAVTKHANRLGFDF
                     QWQTRFYDHIIRNDKSFQNISKYIHNNPANWQNDNFLK"
     gene            3762..5372
                     /locus_tag="PKN53_02915"
     CDS             3762..5372
                     /locus_tag="PKN53_02915"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HOD87936.1"
                     /translation="MTHKEQQQLGKTLWSIADELRGAMNADDFRDYILSFLFLRYLSD
                     NYEVAAKKELGSDYPALAEKDSRIPLSVWYSYNEADIKEFEKQMRRKIHYVIKPEYLW
                     NNIAEMARTQNDQLLVTLEEGFRYIENESFESTFQGLFSEINLNSEKLGKTSAERNKK
                     LCTIIQKIAEGIAGFSTDTDTLGDAYEYLIGQFAAGSGKKAGEFYTPQQISTILSEIV
                     SLDSQDPSAGKKKKFDKILDFACGSGSLLLNVRKRIKENGGTVGKLYGQEKNITTYNL
                     ARMNMLLHGMKDTEFEIFHGDTLLNQWDIFNEMNPAKKIEFDAVVANPPFSLRWDPND
                     TMAEDFRFKSYGLAPRSAADFAFLLHGFHFLGKEGVMAIILPHGVLFRGGAEERIRTK
                     LLKDNNIDTVIGLPSNLFYSTGIPVCILVLKNCKKEDDVLFINASEHFEKGKRQNVLS
                     KQHLKDIVETYKFRKEIERYSRRVSMEEIEKNGYNLNISRYVSTAVEEAKVDLKEVNT
                     KLAEINKNIKTSTDKHNEFLKELGLPPI"
     gene            5449..6468
                     /locus_tag="PKN53_02920"
     CDS             5449..6468
                     /locus_tag="PKN53_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOD87937.1"
                     /translation="MSNKLNINQSEYSVILQQAVAEIRNARTLIAKQINSTTNSVYWN
                     LGKLLFEKQLEEGYGSGVVKQLSVDLKSEFPDMGLSPRNLWDMKRFYERYYQADIKLR
                     QAVAVLPWGHNLLLINKVQSLSAVEFYANEVIVKGWSRDLLLNAIKMDTYSRTQTQIA
                     SNNFSQTLPAINAEYANEVFKSTYNLGFLGITEPVKEIELEKRLIDKIRNFILELGKG
                     FSFIGNQHRLEYNGKEYFVDMLFFHRGLRSLVAIELKIGSFKAEYVGKMNFYLSLLDK
                     LEKDESENQSIGIILCADRDRLEVEIALQDINKPIGVSEYQLLIPKDELQALLVNEIK
                     ASTKK"
     gene            6587..7321
                     /locus_tag="PKN53_02925"
     CDS             6587..7321
                     /locus_tag="PKN53_02925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD87938.1"
                     /translation="MYNFLKRTLKTLFPNISNKFEFTIRRVLSLFYFGNKVYCGVCES
                     NFKRFILLDNAELSCPKCGSLSRQRRMWKFINETIPFKKSDFILHFSPSKILQKKFKK
                     IYPNYVSTDYCGNLKTDKSYDILNIDVADNSFDIIICYHVLEHIIDDMKAMSELNRVL
                     KPGGKLIVQTPFKEGGIYEDYSITTKEDRLKHFGQDDHVRIYSVQGLKERLEKVSFNV
                     EVFSFKEAVDNKFGFKPEEHIILCTK"
     gene            complement(7445..7885)
                     /gene="rpiB"
                     /locus_tag="PKN53_02930"
     CDS             complement(7445..7885)
                     /gene="rpiB"
                     /locus_tag="PKN53_02930"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOD87939.1"
                     /translation="MKKTIGFACDHAGFKLKNHLICFVEDMGYKTMNFGTNSEESCDY
                     PDFAHPLAEAVEAKKCDIGVSICGSGNGINMTVNKHQGIRSALCWNPEISKLARLHND
                     ANICALPARFIDNKTAEEILLNFLNTEFEGGRHINRINKIPIKV"
     gene            complement(7882..8109)
                     /locus_tag="PKN53_02935"
     CDS             complement(7882..8109)
                     /locus_tag="PKN53_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HOD87940.1"
                     /translation="MDEKILIDIVTQKMPYGKYKNRFIYQLPEPYLVWYHNKGFPQGK
                     LGMLLNTIYEIKLNGLEYLLEPIIKNQKNNI"
     gene            8230..8595
                     /gene="rsfS"
                     /locus_tag="PKN53_02940"
     CDS             8230..8595
                     /gene="rsfS"
                     /locus_tag="PKN53_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOD87941.1"
                     /translation="MIKKPKVADAQTLVKVINDSLLEKKGEDILILDLKKTGTSVCDY
                     FVICTGKSETHVNTLAQYVEHNVQEVLKDKPWQKEGYENKEWILLDYVNVIVHVFQAK
                     SRNFYRLEQLWADGEVIAI"
     gene            8698..10776
                     /gene="ftsH"
                     /locus_tag="PKN53_02945"
     CDS             8698..10776
                     /gene="ftsH"
                     /locus_tag="PKN53_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOD87942.1"
                     /translation="MPPNSSGKKPGAPNMTWLYVILFGLFLAFAFMDFGSKVQEIDRK
                     KFETEILPSGDLEKIVVVNKEKVEVYLKPDALSKDKYKDVGKPSSFSFSKNTGPHYFF
                     KIGSVEAFENQLAEAQQDFPPDQKIAAQYETKQNYIGVVLSWLLPFILIIGLWFLIFR
                     RMSGGGGGSGNAIFNIGKSKAQIFDNERRVKINFNDVAGLEEAKVEIMEIVDFLKNSE
                     KYTKLGGKIPKGVLLVGPPGTGKTLLAKAVAGEANVPFFSLSGSDFVEMFVGVGASRV
                     RDLFKQAKAKAPCIIFIDEIDAIGRARGRNANFGGNDERENTLNQLLTEMDGFDTNSG
                     VIILAATNRADILDSALMRAGRFDRQIHLELPDIVEREAIFKVHTRNLKMSKEVDISF
                     LAKQTPGFSGADIANVCNEAALIAARNGKKEIEHQDFLDAVDRIVGGLERKTKIISQT
                     EKRTIAFHEAGHATVSWLLEHANPLIKVTIIPRGRALGAAWYLPEERQLTTKEQLLDE
                     MASTLGGRAAEELVFGEVSTGALNDLERVTKQAIAMVSFFGMSDKIGNLSYYDSSGQS
                     DFGFTKPYSEKTSELIDAEVKAVVEEQYQRAIDILKENMEGLTELAETLLEKEVIFSD
                     DLERIFGKRKGDKESPESVKIEQKQDEDTIAELKEETIPEAKDDKLAETNEDTVIETQ
                     EDIKTETQEN"
     gene            10773..11606
                     /locus_tag="PKN53_02950"
     CDS             10773..11606
                     /locus_tag="PKN53_02950"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOD87943.1"
                     /translation="MKNFYTRTLTGLVFVSVCLSMILIGKWTLLAFFIIADVWLKFEF
                     FRIVKSDNAKALSFTSVFTGVLFILMNFVVLNYNVNTKIFALLLIPVSLIFVEELFRN
                     IENPLRNVAYSILSLIYISLPLVLAQSLSYRVVGNNQIQFLPQILVGIFVLNWTFDSM
                     SYVSGILFGKHKLFERISPKKTWEGSIGGTIFAVLLGYFMSYFIPELSRLDWVVISAL
                     VVVFGILGDLIESLLKRSISLKDSGHSLPGHGGLLDRFDSFIFTIPWVFVYFVIKDLF
                     F"
     gene            11703..13931
                     /gene="purL"
                     /locus_tag="PKN53_02955"
     CDS             11703..13931
                     /gene="purL"
                     /locus_tag="PKN53_02955"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOD87944.1"
                     /translation="MSEIIVTLEIAQTLGVTEEEFEMIKNYLGRTPNYTELSIYSVMW
                     SEHASYKNSIKWIKTLPREGACLLTGAGEENAGLVDIGNGLACAFKIESHNHPSAVEP
                     YQGAATGVGGINRDIFTMGARPVAQLNSLRLGNIDNDRTRWLLRGIVKGIADYGNAFG
                     VPVLGGEVYFDECYNTNPLVNAMSVGIMKKGKMISAIAYGEGNPVYIVGSATGKDGIH
                     GTTFASADLTEDSADDIPAIQVGDPFQEKLLLEATLELAETDAIIGMQDMGAAGIICS
                     TSEMSAKGKCGMKIDLSLVPLRQKNMEAWEILLSESQERMLVVIKKGKEHIVEQIFEK
                     WDLHCARIGEVINEDKLYFYYHGKLVAEVPADSLVLGGGAPVYDREYKEPAYYAENQK
                     FDPNEISQNVDLREVADFLTRHPNIASKKWIYEQYDTMIGTANLTTNRPADAGLLNIK
                     GTNSALAMTTDCNSRYVKADPKVGAMIAVAEAARNVACTGAKPLAITNCLNFGSPMNP
                     EAFWEFVNAIKGMGEACRAFNTPVTGGNVSFYNQTIVDGKAEPINPTPTIGMLGLLKD
                     KLYQTSLAFHRKGDMIYMLGRTSDDIASSEYLYSYHGVKNSPAPEFDLQLEVKLVEVL
                     QELAARNLVNSMHDVSDGGLYIALLESSMPNNLGFDITTPAENRTDAFLFGEAQGRVV
                     VSVSTEKEADFVDFLLEKKFPFNSLGHVTKGELRIDDTSFGFITDEKEKYDNAIGKKM
                     DA"
     gene            13938..15890
                     /locus_tag="PKN53_02960"
     CDS             13938..15890
                     /locus_tag="PKN53_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD87945.1"
                     /translation="MISVNNLTIRFGNFELFSDIGFLINKRDRIGLVGKNGAGKSTLL
                     KVINGDISPNEGNISVPTDIKIGYLPQQMILENAKLSVFEEARTAFQNVLEIQTEIDK
                     LNVQIANYHDYTEDEYSKLIEKLTHLSEHLNIIGADNVEQNIEKVLKGLGFSQTDFQR
                     KISEFSGGWKMRVELAKILLQNPDVILLDEPTNHLDIEAIQWLESFLETYGGAVLVIS
                     HDRAFLDNVTNRTIEINLGKIYDYKVSYSKFKVLQQERLAQQIAAYENQQKLIEDTER
                     FIERFRYKATKANQVQSRIKQLEKLDRLEIDELDNAAMNIKFPPAPRSGDIVVKIDEL
                     SQTYDGKKYILNKIDLIVERGEKIAFVGRNGEGKTTLSKVIIGELEYSGELKIGHNVK
                     IGYFAQNQDELLDPELTVFETLDRIAVGDIRTKLRDILGSFLFSGEDIDKKVKVLSGG
                     EHSRLSLAKLLLEPYNLLVLDEPTNHLDMRSKDILKQALLKFDGSLIIVSHDRYFLDG
                     LVDKVYEFKNHKIRQHIGGIYDFLRKKKIENLKELEQKEKVQASQTKEKVSKNKILYL
                     ERKEIERQRRSLQNKVEKAEKEIERLEKEIETLHNLLSNPEKIDAEEANAVFEKFSKL
                     NSELEHQMDLWEKAGNELHEFEENNS"
     gene            15955..16557
                     /locus_tag="PKN53_02965"
     CDS             15955..16557
                     /locus_tag="PKN53_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOD87946.1"
                     /translation="MEKVKLEVLGLSYSQAQAGAYALILSVENSEERIPIIIGGFEAQ
                     SIAIALEKLSPPRPLTHDLFVSFAEEFEINLKEVVIYKFEEGIFYSKIYFEKDGEIKE
                     IDSRTSDAVAIALRFHSPIYTTLDIVQKTAIILNFTEDDEPPRNQAKPAKPIRKEDAS
                     EYGSMSVSQLKSLMEKAIADENYERASLIRDELKKRNVDF"
     gene            16572..17366
                     /locus_tag="PKN53_02970"
     CDS             16572..17366
                     /locus_tag="PKN53_02970"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOD87947.1"
                     /translation="MKQYLQLLQHVKDNGIIKTDRTGTGTKSVFGYQMRFDLSEGFPC
                     VTTKKLHLKSIIHELLWFLKGDTNIKYLNDNGVRIWNEWADENGDLGHVYGYQWRSWQ
                     TANGESIDQITKLIHDLKNNPDSRRHIVSAWNVADIDNMALPPCHTFFQFYVADNKLS
                     CQLYQRSADLFLGVPFNIASYALLTLMVAQVCGYKPGDFVHTFGDAHIYLNHMEQIEL
                     QLSREPRALPTMKINPEVNDIFSFKYEDFELVNYDPHPHIKGVVSV"
     gene            17377..17856
                     /locus_tag="PKN53_02975"
     CDS             17377..17856
                     /locus_tag="PKN53_02975"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004382106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOD87948.1"
                     /translation="MSFSIIAAVAENYAIGKDNDLLCHLPGDLKRFKQITSGNTVIMG
                     RKTFLSLPNGALPNRRNIVISRSQNLNFKNVEIVASIGEAIKICDADKENFIIGGGEI
                     YKQFLPFVDKIYLTKIHAVFDGDTFFPELNCDDWNVEIEAENTEHEPNFSYINLTKK"
     gene            17970..19100
                     /gene="tgt"
                     /locus_tag="PKN53_02980"
     CDS             17970..19100
                     /gene="tgt"
                     /locus_tag="PKN53_02980"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOD87949.1"
                     /translation="MKFVISHKDKNSKARRGLISTAHGEIETPIFMPVGTLGTVKAVH
                     ITELKNDIKAQIILGNTYHLYLRPGVDIIHKAGGLHKFNSWDKPILTDSGGFQVFSLS
                     QNRKLTEEGATFRSHIDGSKHIFTPENVVEIQRKIGADIIMAFDECTPYPCDYSYAKK
                     SLDLTHRWLLRGWKHFNSTQAIYDYYQTFFPIVQGSVYKDLREDSAKFVSDIGACGNA
                     IGGLSVGEPTQQMYDMIEVVNEILPLDKPRYLMGVGTPQNLLEGIERGIDMFDCVMPT
                     RNARNGMLFTMNGTMNMKNEKWKDDFSPLDECGTTFVDKLYSKAYVRHLIISNEILGA
                     QITSVHNLGFYVELMRIAREKITEGNFSSWKKEMIEKLNNRL"
     gene            19106..20185
                     /locus_tag="PKN53_02985"
     CDS             19106..20185
                     /locus_tag="PKN53_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOD87950.1"
                     /translation="MKILDRYIIKKFIGTYFLAITLIILIAIIFDISEKMDDFIDKQA
                     PLKAIIFDYYFNFIPYFANLFSALFVFISVIFFTSRMAAKTEFVAMFASGISAKRLLL
                     PYFIVAFFITAFSFVLGNYVIPEANKQRFEFQNKYFKRSPGTITSGISHRQIYPGVYF
                     YVGNFNRTNNIGLNMSLEKFDGVVLKSKLTAETMIWDSISESWIVQNYLIRNFLDGRE
                     ELITGHRIDTAFRITPEDFLQRTNIVEEMSVEELNEYIAEQKLSGTSAVIFSEIEKHN
                     RISTPFSAFILTFIGFGLSIKKRRGGLGLNIGVGLLLSFSYILFMRFASVFAINGYFS
                     AALSAWIPNILYFFIAIGIYVKTLK"
     gene            20212..21756
                     /locus_tag="PKN53_02990"
     CDS             20212..21756
                     /locus_tag="PKN53_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOD87951.1"
                     /translation="MSINKKIEALNQKRKEVMLGGGEKAIEKQKESGKLTARERIELL
                     VDKGSFNEYDLFVQHSAREFGMADKVLNGDGVVIGTGTIDGRPICVFAQDFTVVGGSL
                     GYMHAMKIVKIQDLALKMRVPCIGINDSGGARIQEGVDALAGYGEIFYRNTQSSGIIP
                     QLSVILGPCAGGAVYSPALTDYVFVVDNISKMFITGPEVIKTVLGEEITQEDLGGARV
                     HSEITGNAHFFANDEKECFSQIRKLLSFIPHNNKDKNTERKVEKPVANIDINNVVPVD
                     PTKPYDVRNVIKAITDNSDFVEVMEKFAQNMVIGYGRIGGKTVGFVANQPLYLAGVLD
                     VDSSDKAARFIRYCDAFDIPIVTLVDLPGYLPGVDQEHAGVIRHGAKVLYAYSEASVP
                     KLTVILRKAYGGGYIAMSSRHLKADFVYAWPTAEIAVMGPQGAANIIFRKEIAEAADP
                     NEMRKQKVEEYKERFANPYVAASKGYVDEIIVPDETREKIIQALKLSETKAKVKTDKK
                     HGVPPF"
     gene            21768..22082
                     /locus_tag="PKN53_02995"
     CDS             21768..22082
                     /locus_tag="PKN53_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOD87952.1"
                     /translation="MNENRKLKMIVDEVEYETYSTPSFDKKQKWSQPDDKIILSIIPG
                     TIVEILVKEGDKVKAGDAMLVIEAMKMNNKINFLKGGIIDKVLVKPGDIVSKNQELII
                     LK"
     gene            complement(22140..22214)
                     /locus_tag="PKN53_03000"
     tRNA            complement(22140..22214)
                     /locus_tag="PKN53_03000"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22177..22179),aa:Val,seq:tac)
     gene            complement(22469..23374)
                     /locus_tag="PKN53_03005"
     CDS             complement(22469..23374)
                     /locus_tag="PKN53_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87953.1"
                     /translation="MRSIFFAILALLNFNLLIGQDYIEYYNFCNEGDKQIYLKNYEEA
                     LKNFNKAFSLVDYVHANQYKSASLCAAHIGNIEKTYLYAKNAILNGLDPVFLKTKDFK
                     KFRKTEKYKILCDSISIFQKQHLLSINLNYKKEIDSLYYIDQNIIRNNKSCKGNYNID
                     KSKLPSNLFELDSVIFEHLLTLIEKYGFPSEKKIGYEGYSNVWVLFHHNVRLPKNSKY
                     LPILKEALVKGEYLPRNYAWMYDQSLMFKQEEPYFYFGIALVDHLSDDKKMEIDKRRK
                     EHGIKPLESTIVKKRGNGIMQKFLW"
     gene            complement(23697..24374)
                     /locus_tag="PKN53_03010"
     CDS             complement(23697..24374)
                     /locus_tag="PKN53_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HOD87954.1"
                     /translation="MNKRRVFLIPVIALLLAFCAVQIGCKSKKSDDSFTDERDGNTYK
                     TVKIGEQIWMAENLKYLPSVDAPTTGSYTEPHYYVYDYEGNNVEEAKATDTYKTYGVL
                     YNWKAALSACPEGWHLPTNSEWEQLIEFLGGETVAGGKLKSTDSIYWNIPNEGATNES
                     GFNALPGGYREYSGVFSYLNSYGFWYSATEYSSDYAWNRYLDSNNSNAFKYYSKENGF
                     SVRCIKD"
     gene            24646..25956
                     /locus_tag="PKN53_03015"
     CDS             24646..25956
                     /locus_tag="PKN53_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD87955.1"
                     /translation="MKNQTKQKSNVVAIIMMIILFGMISFVTNLAAPMGVVLKEQFEV
                     SNFLGLLGNMANFVAYAVMGIPGGFILQRYGYKKTALLAVGVGFVGVAVQFLSGHVGE
                     NSAFAVYLLGAFIAGFSMCLLNMVVNPMLNALGGGGNKGNQLIQLGGSFNSLMATFTP
                     TFVGILIGATVRAQITDVFPVLYIAMGVFALVFLVLLMVSIPEPHTEKISEPMGGLLK
                     GALKFRHFVLGAIGIFIYVGVEVGIPGTLNLWLSDATNPQGAEVGKTIAGFVVGTYWF
                     LMLIGRLLGAALGSKISSRAMLTVASSVGLVLLLLGVFMSPEIKVSLPVLQRSDAGAL
                     SFGLANVPINAMFFVLIGLCTSIMWGGIFNLAVEGLGKYLQVASGIFMTLVCGGGILP
                     AIQSWVADMAGYQLSYWVVIAGFVYLLFYALVGSKNVNKEIVVK"
     gene            25971..26927
                     /locus_tag="PKN53_03020"
     CDS             25971..26927
                     /locus_tag="PKN53_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOD87956.1"
                     /translation="MDKKYVVGIDIGGTNTVFGIVDARGNVLAKSSVKTAVHQDVNLY
                     INDIYEEFLKIAKDFGGIGNIRGIGIGAPNGNYYTGNIEFAPNLIWKGIVPLSKLVSE
                     KFGVPAVVTNDANAAAIGEMTYGAARGMKNFIIITLGTGVGSGIVIDGKLVYGHDGFA
                     GELGHTCSVRENGRQCGCGRKGCLETYASANGLARTAREFIETRKEPSLLRNIPCESI
                     TSKDVYEAAKQGDKLAIEIFEFTGKILGEALSDFVAFSAPEAIILFGGLAKSGDFIIK
                     PTVEHMEKNLLVLWKNKVKVLFSALKEADAAILGAGALTWEV"
     gene            27203..27637
                     /gene="dut"
                     /locus_tag="PKN53_03025"
     CDS             27203..27637
                     /gene="dut"
                     /locus_tag="PKN53_03025"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOD87957.1"
                     /translation="MKVKIVNKSKHALPQYSTEFSAGLDLRANLDEAIVLKPLERKLI
                     PTGLFIELPVGFEAQIRPRSGLALKHGISVLNTPGTIDADYRGEIGVILVNLSDKVFV
                     VQDGERICQMVIAKHEQAEWIEVEQLVETERGAGGFGHTGVK"
     gene            27648..29378
                     /locus_tag="PKN53_03030"
     CDS             27648..29378
                     /locus_tag="PKN53_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD87958.1"
                     /translation="MSKTKYINFFAILLVLLISSCSTLKKNEKQPVDPRIKNFNFNYY
                     FLEANKQKILGNYGDALELYALASNQDKSDAASYYEIAGILNLAQDYSSALEYAKKAV
                     KHDKFENKYYLLLLASIYSNNNLHKDAIKVFEKLIEIEPLNIGYYFQISSLHQMQKKY
                     SDAIKILNKAEATFGVSDVICLEKENIYIKMGEPDKAVAEIKKLCDAYPEKTINKILL
                     AESYVNVGKRKEAEEIYNQVCDLEMDDDIAYFSIADFYLSVGKYNKAFELINKGILSQ
                     NVDLNIKLNLILSLLNNIKVGDTDITNKLGEFIEALVLQYPDDLIVRVLKSDYLLFVG
                     DFKNAQKEYEFILEHEKTKIEIWQQAISIDVALQDMNSLYTHSKEAVELFPLVLDFYR
                     YYIISAYATSNFQDVVEAVDFASPYVEKNHELYIDFLSMQADAYYKLEEFHKSDSVFD
                     LILYKDSENIQALNNYSYFLALRNENLDKALEMSTKLMSLAPNNPSFLDTHAWVLYKH
                     KRYADALIFINQALIQDSTNVTFLEHKGDILFRIDKVDQALEFWEEAYKNNGDNEQLK
                     EKIKQKKILD"
     gene            29378..30295
                     /locus_tag="PKN53_03035"
     CDS             29378..30295
                     /locus_tag="PKN53_03035"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HOD87959.1"
                     /translation="MFKRQNIIIIFLLLLVLVSCKAVRFINGGKGETIRPHRLYRHLE
                     ANRFDFENVNLKFSANVDFGESSQSFGGNIKIKRDSIIWISLRSFNVEGLRVVITKDS
                     VKYINRLDNTYYLGEFSFLQERFAIDVDYNALEAILTNNFFFYPSSEDTTKSVSDFKL
                     CEDSVYHCMASISKRKYNRFFVDEKGADRIERRLERESQQDSVNRFWQRGEFEDYYYQ
                     IVKVMPGLYRVKEMFLENYVQQQSLSIRYEDQVLEDTQFFPSKIFVELSSFIFFVELK
                     LNIESVGVNNTDMSFPFKVTNKYKEISIK"
     gene            30292..31488
                     /locus_tag="PKN53_03040"
     CDS             30292..31488
                     /locus_tag="PKN53_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOD87960.1"
                     /translation="MKRLKYIILIFVVFLSASHIFAQSKSELEKKKNKTQQDINYTNK
                     LLKETQKTQKESYNNLLLINKNIELRRSIINDISYEIKLINGKIADIELIISIMEEDL
                     ENLKTHYANMIRVAWKNTNKYNNIMFILASEDFNQAYLRLKYMQQLANYRNKQFKAIT
                     ALTDILAIQKTKLTDLKNKNNNLLTEQRNEERTLKNDQVKQESTIKKLKAQESDLKKK
                     LQQQQKQMADLQREIERIIREEAKRNTNVTTGKFELTPEAKLVGTNFEKNKGKLPWPV
                     ERGVIVSGFGKQKHPVLANIEIDNRGIDISTTVGASIRAVFDGDVKQVLAMPGMQNVV
                     IIMHGEYMSVYSHLETVLVSKGDKVLAKQVIGKVYTDKTENKTILHFEVWKSTAAVNP
                     TYWLSK"
     gene            31490..34801
                     /locus_tag="PKN53_03045"
     CDS             31490..34801
                     /locus_tag="PKN53_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87961.1"
                     /translation="MTDNFKILLDKLNVFIKKYYINKLLKGLIISLSIYVAWYLIVVV
                     AEYFGRFSSSFRTVLFIVTLASFAFILIKMIIIPIFNLLKIGKTISYKDASKIIGSHF
                     PEVADKLQNTLELKELVKTNPEFSDVLIASINQRIEKLKPIPFLSAVSFKSNLKYLKY
                     LGIIFLVAMLIFLFWPRMYSEATERIINFDKHYIEPAPFTFEVLNDSLLVKKGKDFVL
                     KVSAKGKYSPDQVTLNYMGNSFYMEKESVGIFTYKFKNLNNDIKFNLSAVDVVSQPYE
                     IKVLPSPIIVDFKAIVKAPAYTNIEEKVYNNSGDLTLPIGSEVVWNFNTSNLLSLHLV
                     FDTVQVKATKTNEVYSVKRRILRTGTYSIVLANEYFNENTGVNYQLNAIPDLYPSIDV
                     KKVVDSTHLSIVYFNGFIDDDYGFSSLSFYCKPGEKSDSLIRINIPFAKSISSQDFYF
                     AFDFSKIDVEGQTISYYFEVADNDGVNGAKKTRTQKMDFVIPSAEDLQQMTSKANEET
                     ESKIDLAKDINSQLRKNIEELQKKLLSEQLTPFERNQMLQQIMDNQSDLENLMKDIKK
                     EQSQVQQYKDQFSKNEELLKKQQEINDLFDSLMDDEMKKMMEELQKLMEEFKSDDFFK
                     LADQMKFDYEELEKQMDNTLELLKKTEIEERLKNTASQLEELSKEHEKLSEETTDKKS
                     DKQDLLQKQKEHEAAFDKIKEDYKEILEKNSELKEPMDLESFEEEMQEISEGLEQSES
                     EMKDEKMSKASKSQKQNSEKMQELSQKMESAMQEQEQEEMSENIEDIRQVLENLITFS
                     FNQEEILTEQKGLSPRDPRYREFILKQKNIEDDFKIIRDSLNAMSGRIPQLGPMVEKE
                     IRTIHKNLSTIMDEIGENRRYLVEASQQLVMTSANNLALLLLEMMEQMQNQMANSKSG
                     DGQCNNCKNQGDGSPKGKMRDLQQGMKQQMQDMIDMMKDGNMKKGGAKQSEQLAKMLM
                     QQEMMQQMLNDVMNSGLSPESAKILQEINRMMDQNLSDIINRNINPTTIQRNEQILTR
                     LLQAENSEREREIDKKRKSNEATNYKLSNPDEIFKEKEKEIRFNELLQKSNLKLRTFY
                     NDKYKEYLKKLNQDQ"
     gene            34801..35220
                     /gene="ybeY"
                     /locus_tag="PKN53_03050"
     CDS             34801..35220
                     /gene="ybeY"
                     /locus_tag="PKN53_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOD87962.1"
                     /translation="MAVKFFAEDVEKPALKYRSIKQWIASEIEKENFDIGNINYIFCS
                     DEYLLKMNIKHLEHDYYTDIISFDYCDGNLISGDLFISLDRIKENAVLYGTNESEIYR
                     VMIHGILHLCGYKDDTEEESENMRQRENEALLRLDLI"
     gene            complement(35221..37980)
                     /locus_tag="PKN53_03055"
     CDS             complement(35221..37980)
                     /locus_tag="PKN53_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87963.1"
                     /translation="MKEKIVKNQKRTNIKHFKEYLILIALSFLLYGLSINNGFNIDDD
                     YVYENHELVQKGIKGIPEIFSSRYNTKDEQYFGYRPLTIAVFAIEYQFFGSNPHSAHF
                     FNILYYAIACALLFYFLNLIFKEKYPDNYSFLSFLIVLLFATHAIHTEVVLSLKNREE
                     ILSFIFGIMSSICAIKYFDNKKIQHLLFSIFALILAFLAKESALVFIVLIPLTIIFFK
                     TEIKILPNYKFTSPKFSPNKKHNKIIVYTFLVFIFLFLLLSPEFKITSNKSVQLHNLY
                     ININEYVVWGIFFICYLYLLYYRRKINCPVKFNVRNILLWIANVITLVLLLFTKSDLA
                     GLLMFFIPLFTLKIKDKEEYTQKSKVKIFDGISKKLIFSTLAFLLIAGVALFITYYIP
                     KKSLPEVNAPVFMWQNPIFGFGASIFDKMALALYSLIYYFKLLIIPYPLRFYYGYKMI
                     PIVDMSNPIVLLSLVINIVLLIWALKNFNKRNVMSFGYLFLLISIFPLANTFFPLTGI
                     IAERLLFTPSLGFSIIIGFLLFKITKINLNLSLKKTDKNLIFALALVFILPNSIITIN
                     RNKDWKDRRSLFSHDIKYLENSAKANNLYGNILISEVYAAIKTKQSINSFANQIKVAE
                     THFKKAIEIDSSYSNPWHNLAYMNMIIYKNYELAEQQFTRCIQADSTIAAVYLNRGIV
                     NYHLNNYEQSIQDLNVYISKYHNYKDKEWDKAYLFMGKSYFELKDTTKCNENYIKALN
                     NIKIENLNLTVLLDIINNFAITHDYENLVKSLEYLKNENLNAKGLFDMMTYYANIKDF
                     NYIAKVIEFYNKPNNESTSLNDIIKHFETSKDYENIIKTIDFKIHLNPELDANYVDKG
                     NYYLLSGNTAKAIENWEIAFEKNKANFNIGMTLSNYFKENNNFEKANYYYSEANKFKI
                     MKKSQ"
BASE COUNT        13747 a         5173 c         6761 g        12524 t
ORIGIN      
        1 gcagtcaagt ctatttctat aaaaaaagta caaactacaa ccaataatta aaatatatga
       61 gcgaaattgt actatatcaa tccaatgaac ttgccaaaca tgttgaagta ataattgatg
      121 agaaaacaga aaccatttgg ctcactcagc aacaaatggc agatttgttt ggcgttcaac
      181 gtcctgctat tactaaacat ttaggcaata tatttaaatc tggcgaatta gaagaaaatg
      241 tggttagttc cattttggaa cataccactc aacacggtgc tatagaagga aaaaaacaaa
      301 aaacaagtgt gaagtattac aatattgatg cagtattatc tgttggttat agagttaatt
      361 ctgctcgtgc aactcaattt cgtatatggg caacgagaat attaaaagat tatttgctta
      421 aaggttatgc tttaaacaca cgaataaata ggcttgaaaa taattttgaa aaccttaaaa
      481 ataaagttaa cgaaatagat cttcagataa acacacacct cattcccact caaggcgtat
      541 tctttgatgg tcaagtattt gatgcttatg agttagcttc caaaattatt cgttctgcta
      601 aggaaagtat tgtattgatt gataattaca tagacgaaac tactttaact catttatcta
      661 aaaaagcaaa aacaataaaa gtgcttttac ttactaaaac tatttctaaa caattgactt
      721 tagatgtaaa aaaagccaac gagcaataca aaaattttga aataaaaact tttgccaaaa
      781 gccacgaccg ctttttaatt attgacaata cagaagtata tcatttggga gcttctttga
      841 aagatttagg caaaaaatgg tttgcttttt ctaaaatgga taaaaactca gtagaaacaa
      901 tcttaaaaga gatagaaaaa tgaaagaaat agaaaaacaa ccccccgaat tatatagcga
      961 tgtttgtcga attatagaaa acacccgcac tcgtttagct actaccgcga atgccgaagt
     1021 gtgtttaatg aattggcagg taggtttacg cattaaaact gaaattttaa aagaccaacg
     1081 tgccgagtac ggtaaggaag ttataaagaa tttagccaaa cttcttactg aaaaatatgg
     1141 caaaggttgg agtttgtaca aactgcaaca ttgtgtacgc agtgcgtacg tttttgctga
     1201 agatgagatt atgtacgcag tgcgtacaca attaacttgg acgcatttgc gctcgttgat
     1261 gagcattaac gatgaactaa aacgcagatt ctatatggaa atggtgcgta ttgaacactg
     1321 ggacacaaga acgttggacg aaaaaattga cggaatgttt tacgaacgta ctattttgag
     1381 ccgaaaaccc gaagaattta ttaaacagga actacaacaa atacaaacca cacacacgct
     1441 tacaccagat gtcgttttca ggagcagtta ctttttagat accttggggc ttgccgatac
     1501 ctattccgaa aaagatttgg aagatgccat acttataaat ctgcaatcgt ttattaaaga
     1561 aatgggtagc gatttcgctt ttttagaccg ccaaaaacgt attaccattg atgccgtaga
     1621 ttaccgcatt gacttattgt tttttcaccg tggtttaaaa cgcttggttg ctatcgattt
     1681 aaaacttggc aaatttaaac ccgaacacga aggacaaatg ttgttgtatt tgcgctatct
     1741 caataaaaac gaacgtaaag aaggcgaagg tagcccaata ggcttgatac tttgtagcga
     1801 aggtaatacc gaacacattg aatatttgat gttagaagaa agcaatataa aagtagcaca
     1861 atattacaca cagctacctg ataaaaaaat attgagtgaa aaattacata aagcaatagc
     1921 tatagcaaga gaagcaaaac aatcgaaata atgagtaaaa caaaaaaaat aatacccgaa
     1981 tttaggtttc cggagtttaa aggagaatgg tctctggaag aacttgggca atgtcttgat
     2041 tatttacaac caacaccttt cttggttgac aacaccgact ataatgacga atacgaaatt
     2101 cctgttttaa cagcaggcaa gtcttttatt cttggataca caaatgaaca agaaggtatt
     2161 ttcaacgaaa atttgcctgt tataatcttt gatgatttta caacggcttc tcagtttgtt
     2221 gactttccgt ttaaaacgaa atcttcagct atgaagattt tacttgcaaa aaatgataat
     2281 gacatcaagt ttgtgtatga gtctatgcaa accctgaatt atgaagtagg agttcataaa
     2341 cggcattgga tttcagtttt ttctaaactt cgaatagcag ttccaaaacc tcaagaacaa
     2401 caaaaaatag cctcctgcct ttcttcatta gatgagctga tagccgccca caaggataag
     2461 ctggacgccc tcaaagacca caaaaagggt ttgctgcaaa accttttccc acaagatggc
     2521 gaaaaagtgc ctaagttaag gtttaaggag tttgaaaata gtggggagtg ggaggagaag
     2581 aaaatttctg accttggaga gacaataagt ggactaacag ggaaaagtaa agatgatttt
     2641 ggtgcaggaa aaccttttgt tacatacaag caagtttttg ataactctga gataaatttt
     2701 gaacaatgtg aaagagttca aatcagtgat gatgaaaatc agacttcttt gcaacaagga
     2761 gatattttgt ttaccacatc ttctgaaaca ccaaatgagg ttggatttgc ttctgtcttg
     2821 atagatttcc ctaaagagaa aacctatttg aacagttttt gctttgcttt aagaccattt
     2881 aatatagaaa aaacgcaacc acaattttct agatacttat ttcatagtcc tatttataga
     2941 aggtctgtaa cagcgattgc acaaggaagt acaagatata acatttcaaa gggggcattt
     3001 gttgaaattc aagttcctat tccagcccct accgaacaac aaaaaatcgc ctcttgtctt
     3061 tcctctttag atgaggtaat agaggcacaa gtagcgaaaa tagaacagtt gaaattacat
     3121 aaaaaaggat tgatgcaggg attgtttcct aaaataaatg attaaaagat ggaaaaattt
     3181 cagaataaat atcgtgtatc ttcttttaga ttaaaaaatt ggaattattg ttcggaagga
     3241 gattatttta ttaccatttg cacccaaaac atggaacatt attttgggaa aattgaaaat
     3301 gctaaaatgc aattatcgcc agttggtgta attgccgata ttttatggta tcaaatacca
     3361 tatcattcaa aaaacgttga attaggtgcg tttgtggtaa tgcccaacca tattcatggg
     3421 atattgacaa ttgtaaaaaa taataataaa aattgtttag ataaccctaa cataccaacg
     3481 caaccaacga ttccgttgtc gtcagcattg cccatttcgt ctatgtcgta ttccaaaaaa
     3541 aatgaacgga tggcaaaaat ttcgccgaag aaaaattcta tttcgagcat tattcgttcc
     3601 tacaaatctg ccgttaccaa acatgcaaat cgtttgggat ttgattttca atggcaaacc
     3661 cgtttttacg accatatcat tcgtaacgac aaatcattcc aaaacatttc caaatatatt
     3721 cacaataacc ccgccaattg gcaaaatgat aattttttaa aatgacacat aaagaacaac
     3781 aacaactcgg taaaaccctt tggtctatag cagatgaact gcgaggagca atgaatgcag
     3841 atgattttcg tgattatata ctttcgtttt tatttcttcg atatttatcg gacaactatg
     3901 aagtagctgc taaaaaagaa ttaggttcag attatccggc attagctgaa aaagatagtc
     3961 gtattccttt atcggtttgg tattcataca atgaagccga tattaaggag tttgaaaaac
     4021 aaatgcggcg taaaatccat tatgtaatta aacctgaata tttgtggaac aacattgcag
     4081 aaatggcaag aacccaaaac gaccaattgc ttgttactct agaggaaggt tttaggtata
     4141 tagaaaacga atcgtttgaa agcacctttc agggattatt ttccgaaatt aatttgaact
     4201 cggaaaaatt aggaaaaaca tctgccgaaa gaaacaaaaa actttgtact attattcaaa
     4261 aaattgctga aggaattgct ggtttttcaa ccgatacaga tacgcttggc gatgcttacg
     4321 aatatttgat aggtcagttt gctgcaggtt cgggcaaaaa agcaggcgag ttttacactc
     4381 cacaacaaat ttcaaccatt ctttccgaga ttgtaagttt agacagtcaa gacccaagtg
     4441 caggtaaaaa gaaaaaattt gacaaaatcc tcgattttgc ttgtggttca ggctcattat
     4501 tactcaatgt aagaaagcgg ataaaagaaa atggcggtac tgtcggcaaa ttatacggtc
     4561 aggaaaaaaa tattacgacc tataaccttg ctcgaatgaa tatgcttttg catggaatga
     4621 aagataccga gtttgaaata tttcacggcg acactttgct caatcaatgg gacattttca
     4681 acgagatgaa cccggcaaaa aaaattgagt ttgatgcagt agttgcaaat ccacctttta
     4741 gtttacgttg ggacccaaac gacactatgg cagaagattt tcgcttcaaa agctacggat
     4801 tagcaccaag atcagcagcc gattttgcat ttttattaca tggttttcac ttcttgggga
     4861 aagaaggggt tatggcaatt atactgcctc atggagttct tttccgtggc ggtgccgaag
     4921 aaagaatacg cacaaaactt ttaaaagata ataatattga tactgttata ggattgccat
     4981 ctaacttgtt ttattcaaca ggaattcctg tttgcatttt ggttttgaaa aattgtaaaa
     5041 aagaagatga cgttttgttt ataaatgcga gtgagcactt cgaaaaagga aaacgacaaa
     5101 atgtactttc aaaacaacat ttaaaagata tagttgaaac ctataaattt agaaaagaaa
     5161 tagagcgtta ttctcgaaga gtatcaatgg aagaaattga aaagaacgga tacaacttaa
     5221 atatttcgag atatgtaagt acagcagttg aggaagcaaa agttgaccta aaggaagtaa
     5281 atacaaaatt ggctgaaata aataaaaaca taaagacaag taccgataag cacaatgagt
     5341 ttttgaaaga gctgggtttg ccgcctattt agatgtaaat tatttattta aaaagggact
     5401 atctcaaaac aaattttaaa taacagatta ttatatcgaa aacactacat gagtaataaa
     5461 ttaaacataa accaaagtga atatagtgta attttgcaac aagctgttgc agaaattcgt
     5521 aatgctcgta cactaattgc aaagcaaata aacagtacaa caaattcagt ttattggaat
     5581 ttgggaaaat tactttttga aaaacaactt gaagaaggtt acggcagtgg tgtggttaaa
     5641 caattatccg ttgacctcaa aagtgaattt ccagatatgg gactttcacc tcgaaatctt
     5701 tgggatatga aacgtttcta tgagcgttat tatcaggcag atataaaact gcgacaagct
     5761 gtcgcagttt tgccgtgggg acataatttg cttttaatca ataaagtgca gtctctttcg
     5821 gcggttgagt tttatgccaa tgaagtgata gttaaaggtt ggagtagaga tttactactc
     5881 aacgccataa aaatggacac ttacagtcgg acacaaacac aaattgcaag caataatttt
     5941 agtcaaacac ttccagcgat aaatgccgaa tatgctaatg aagtatttaa aagtacttat
     6001 aatttaggtt ttttgggaat taccgaacca gtaaaagaaa ttgaactcga aaaacggctt
     6061 attgacaaaa ttagaaattt tattttagaa ttgggcaaag gctttagttt tataggcaac
     6121 caacatagat tggaatataa cggaaaagag tattttgtag atatgctttt ttttcatagg
     6181 ggattgcgtt ccttggtagc tattgaatta aaaataggaa gtttcaaagc agaatacgta
     6241 ggtaaaatga atttttatct ttcattactc gacaaattgg aaaaagatga aagcgagaat
     6301 cagtctatag gtattattct ttgtgctgat agagaccgcc ttgaagtaga aattgccttg
     6361 caggatataa ataaaccaat aggagtatct gaatatcaat tattgatacc aaaagatgag
     6421 ttgcaagcct tgttggtaaa tgaaataaaa gctagtacaa aaaaatgata attaacggtg
     6481 tttttttcca aaattcctag ctatttgtta ttaaattttt tttgtgcatt tttgcaaaac
     6541 tatagtatgg ttttagattt ttagaaataa agctaaaaaa ttttttatgt ataatttttt
     6601 aaaaagaaca ttaaaaacgc ttttcccaaa tatctcaaat aaatttgaat ttactattcg
     6661 aagagtgctg tcgttatttt attttggcaa taaggtttat tgtggtgtat gtgaaagtaa
     6721 ttttaaacgt tttattcttt tagacaatgc agaattaagc tgtcccaaat gtggaagtct
     6781 aagcaggcaa agaagaatgt ggaagtttat taacgaaaca attccattca aaaaatcaga
     6841 ttttatattg catttttctc catcaaaaat tcttcaaaaa aagtttaaaa aaatttatcc
     6901 caattatgtt tcaacagatt attgtggtaa tctaaagacc gataaatcat atgatatact
     6961 gaatattgat gtggcagata actcttttga tattataatt tgctaccacg ttttagaaca
     7021 tattattgat gatatgaaag ctatgtctga attaaatcgg gttttaaaac caggtggtaa
     7081 attaattgtg caaacacctt ttaaagaagg cggtatttac gaagactatt caattacaac
     7141 aaaagaagat agactgaaac attttggtca agacgaccat gttagaatat attcagttca
     7201 aggactaaaa gaacgacttg aaaaagtttc ttttaatgtt gaggtttttt catttaaaga
     7261 agctgtagat aataaatttg gctttaaacc cgaagaacat ataattttat gcacgaagta
     7321 aaataagctt tagactttac cttcggtgag ctcgcaagct cggttcgaca cttcaactac
     7381 gttcagtgca tcgcttttaa cttttgtgcc cttttgtgtc ttttgtggtt taactctttt
     7441 aactttaaac ttttattgga attttattaa ttctattaat atgccttcct ccctcaaact
     7501 ctgtatttaa aaagtttagt aaaatttctt ctgctgtttt gttatcaata aatcttgcag
     7561 gcaaggcaca tatatttgca tcgttgtgta gtcgtgcaag ttttgaaatt tcaggattcc
     7621 agcataaagc agaacgaata ccttgatgtt tgttaactgt catattaatc ccgtttccgc
     7681 taccacaaat agatacgcca atatcgcatt tttttgcttc tacagcttct gccaaagggt
     7741 gtgcaaaatc gggatagtcg caactttctt ctgaatttgt gccaaaattc atagttttat
     7801 aacccatatc ttcaacaaaa caaatcaaat gatttttcaa cttaaaacct gcatggtcgc
     7861 atgcaaaacc tattgtcttt ttcatatatt atttttttga tttttaatta ttggctctaa
     7921 caaatattct aatccattta actttatttc gtaaatagta ttaagtaaca ttcctaattt
     7981 gccttgcgga aatcctttgt tgtgatacca caccaaataa ggctctggaa gctggtatat
     8041 aaatctgttc ttatattttc cataaggcat tttctgagtt acaatatcta ttaaaatctt
     8101 ttcatccata ttgcaaaaaa atataatttt taagattttt caaagtataa taaagaattg
     8161 ttaaaaagta tttttttatt atcatctata gtttataatt ttctatattt gcaaaaaata
     8221 aaattataaa tgataaaaaa accaaaagtt gcagatgctc aaactttagt aaaagttatt
     8281 aatgattcac ttttagaaaa gaaaggcgaa gatattttaa ttttagattt gaaaaaaaca
     8341 ggaactagcg tttgtgatta ttttgtgatt tgcacaggga agtcagaaac tcatgtaaat
     8401 acactagctc aatatgtaga acataatgtg caagaagttc ttaaagataa accttggcaa
     8461 aaagaaggtt acgaaaataa agaatggatc ttattagatt atgtaaacgt tattgttcat
     8521 gtttttcaag ctaaatcgcg taacttttac cgtttagagc aattatgggc agacggagaa
     8581 gttattgcaa tataatttat gcaacaattt gtgtttttaa ttgtttgtta ataataaaaa
     8641 tgtaaaatga aaaaaaacaa taataataaa ggatttaata ataaacaaaa gcctaatatg
     8701 ccaccaaata gtagtggtaa aaagcctgga gcgccgaata tgacatggct atatgttatt
     8761 ttgtttggat tatttttggc ttttgccttt atggattttg gctcaaaagt tcaagaaatt
     8821 gatagaaaaa agtttgaaac agaaatactc ccttctggag accttgaaaa aattgtagta
     8881 gtaaataaag aaaaggttga agtttattta aaacctgatg ctttaagtaa agacaaatat
     8941 aaagatgttg gaaaaccaag ttcttttagt ttttctaaaa atacaggacc tcattatttt
     9001 ttcaaaattg gttctgtaga agctttcgaa aatcaattag cagaagccca acaagatttt
     9061 ccgcctgacc aaaaaatagc agcacaatac gaaaccaaac aaaattatat tggagttgtt
     9121 ttaagctggc ttttaccttt tatcctaatt ataggattgt ggtttttaat attccgaaga
     9181 atgagtggtg gaggcggtgg tagcggaaat gcaattttta acataggaaa atctaaagca
     9241 caaatttttg ataatgaaag aagagttaaa attaatttta acgatgttgc tggacttgaa
     9301 gaggcaaagg ttgaaattat ggaaatagta gatttcctaa aaaattcaga aaaatacaca
     9361 aaattaggag gtaaaattcc taaaggagtt ttgttagtgg gacctcccgg aacaggaaaa
     9421 actttacttg caaaagctgt tgccggcgaa gctaatgtgc ctttcttttc gcttagcggt
     9481 tccgattttg ttgaaatgtt tgtaggcgtt ggtgcatcta gagtgcgcga tttgtttaaa
     9541 caagcaaagg caaaagctcc ttgtattatt tttattgatg aaattgatgc aataggtaga
     9601 gcaagaggaa gaaatgctaa ttttggcgga aatgacgaaa gagaaaatac tctaaatcag
     9661 cttcttaccg aaatggacgg ctttgataca aatagtggtg taattattct tgccgcaaca
     9721 aatagagcag atattttaga cagtgcttta atgcgtgctg gtcgtttcga tagacaaatt
     9781 cacttagaat tacctgatat tgttgagcgt gaagctatat ttaaagtgca tactcgcaat
     9841 ttaaaaatgt ctaaagaagt ggatattagc tttttagcta aacaaacacc aggatttagc
     9901 ggagctgata ttgcaaatgt ttgtaacgaa gctgctttga tagctgcacg aaatggcaaa
     9961 aaagaaattg aacatcaaga ttttcttgat gcagttgaca gaattgttgg aggtcttgaa
    10021 cggaaaacta aaatcatttc tcaaacagaa aaaagaacta ttgcttttca tgaagcagga
    10081 cacgcaactg ttagttggct cttagaacat gcaaatcctt taattaaagt tacgataatt
    10141 cctcgcggta gagctttggg agcagcttgg tatttgcccg aagaaagaca actcacaact
    10201 aaagaacaac ttttagacga aatggcttct actcttggcg gtagagctgc cgaagaactt
    10261 gttttcggag aagtttcaac tggtgcctta aacgatttag aaagagtaac aaaacaagct
    10321 atcgcaatgg taagcttctt tggtatgagc gacaaaatag gtaacctttc ttattacgac
    10381 tcaagcggac aaagcgattt tggatttaca aaaccatata gcgaaaaaac ttctgaactt
    10441 attgacgcag aagtaaaagc tgttgtcgaa gaacaatatc aaagagcaat tgatatttta
    10501 aaagaaaata tggaaggact aacagaactt gctgaaactt tattagaaaa agaagtgatt
    10561 tttagtgatg atttagaaag aatttttgga aaacgtaaag gcgataaaga aagtccagaa
    10621 tcagtaaaaa ttgaacaaaa acaagatgaa gatacaatag cagaattaaa agaagaaaca
    10681 ataccagaag caaaagacga taagctagca gaaacgaatg aagacactgt aatagaaaca
    10741 caagaagata taaaaacaga aacacaagaa aattgaaaaa tttttataca agaactttga
    10801 caggcttagt gtttgtaagc gtttgcttat caatgatatt aattggtaag tggactttgc
    10861 ttgctttttt tattatagca gatgtttggt tgaaatttga attttttaga atagtaaaat
    10921 ctgataatgc aaaagcttta agttttacaa gtgtttttac tggtgttctt tttattctaa
    10981 tgaattttgt agtccttaat tataatgtaa atactaaaat atttgcactg cttttaattc
    11041 ctgtatcatt aatttttgtt gaagaacttt tccgaaatat tgaaaatcca cttcgtaatg
    11101 ttgcatatag cattttgtcg ttaatataca ttagtttacc tttggttttg gctcagtcct
    11161 tgagttatag agttgttgga aataatcaaa ttcaattttt gccgcaaatt cttgtaggaa
    11221 tatttgtttt aaattggact tttgactcta tgtcttatgt ttcgggcatt ttatttggaa
    11281 aacacaagct ttttgaacga atttcgccta agaaaacatg ggagggaagt ataggaggaa
    11341 cgatttttgc agttttgttg ggttacttca tgtcttattt tattcctgaa ttaagtcgat
    11401 tggactgggt tgttatttct gcacttgttg tagtttttgg tatcttaggt gatttgattg
    11461 aatctttact taaacgcagt attagcttaa aagattctgg acactcactc ccaggtcatg
    11521 gtggattgtt agacagattt gatagtttta tttttacaat cccttgggtg tttgtatatt
    11581 ttgttattaa agatttattt ttctaataaa cttcttttaa tatcttttca aaacttaata
    11641 tttttaaaat tgaaaattca caaaatttat attagttttg ttttttgaaa aaaataaaag
    11701 ctatgtcaga aattattgtt acactcgaaa ttgcacaaac acttggtgtt acagaagaag
    11761 agtttgaaat gattaagaat tatttaggtc gcactcctaa ttacacagag ttaagtattt
    11821 attcagttat gtggagtgag catgcttctt ataaaaattc tataaaatgg ataaagactt
    11881 tgcctcgcga aggtgcttgt ttgctaactg gcgctggaga agaaaatgca ggtcttgttg
    11941 atattggaaa tggtttagct tgtgctttca aaatagaatc acacaatcac ccttcggcag
    12001 ttgaacctta tcaaggtgca gcaactggtg taggtggaat taatcgcgat atttttacaa
    12061 tgggtgctcg ccctgtagct caattaaact ctttgaggct tggaaatatt gacaacgaca
    12121 gaaccaggtg gttgttgcgt ggaattgtta aaggaattgc tgattatggt aatgcttttg
    12181 gagttcctgt tttaggtgga gaagtttatt ttgacgaatg ttataacaca aaccctttag
    12241 taaatgcaat gtcggtggga attatgaaaa aaggaaaaat gatttctgct attgcttacg
    12301 gagaaggaaa tcctgtttat attgtcggct ctgcaacagg aaaagacggt attcatggaa
    12361 ctacttttgc ttctgctgat cttacagaag attctgctga cgatatccct gctattcaag
    12421 taggcgaccc tttccaagaa aaacttttac ttgaagcaac attagaactt gcagaaactg
    12481 acgcaattat cggtatgcaa gatatgggag ctgctggaat aatttgttca acaagcgaaa
    12541 tgtctgcaaa aggtaaatgc ggaatgaaaa tagaccttag tcttgttcct cttcgccaaa
    12601 aaaatatgga agcttgggaa attttgctta gcgaatctca agagcgtatg ttagtagtta
    12661 ttaaaaaagg aaaagaacat attgtagaac aaatttttga aaaatgggat ttacattgtg
    12721 caagaattgg agaagtaata aacgaagata aactttattt ttattatcat ggtaaattag
    12781 ttgccgaagt ccctgctgat agccttgtcc ttggtggtgg agctccagtt tatgatagag
    12841 aatataaaga acctgcttat tatgcagaaa atcaaaaatt cgacccaaat gaaatttctc
    12901 aaaatgttga tttacgcgaa gttgcagatt tcttgactcg tcatcctaac attgcttcta
    12961 aaaaatggat ttatgagcaa tacgacacaa tgattggaac agcaaatctt acaactaaca
    13021 gacctgcaga tgctggactt ttaaatatta aaggaacaaa ttctgctctt gcaatgacaa
    13081 cagattgtaa tagcagatac gtaaaagcag atccaaaagt tggagctatg attgctgttg
    13141 ccgaagctgc tcgaaatgtt gcttgtacag gtgctaaacc gcttgcaatt acaaactgtt
    13201 taaattttgg ttcgccaatg aatccagaag ctttttggga atttgtaaat gcaataaaag
    13261 gaatgggaga agcttgtaga gcatttaata ctcctgttac tggcggaaat gtttctttct
    13321 ataatcaaac tattgttgat ggaaaagcag aaccaataaa tccaactccc acaattggaa
    13381 tgttgggatt gttgaaagac aaactttatc aaacatcatt agcgtttcac agaaaaggcg
    13441 atatgattta tatgcttggg agaacaagtg atgacatagc ttcttctgaa tatttatatt
    13501 cttatcatgg cgtgaaaaat tcgccagcac cagaatttga tttacaactt gaagttaaat
    13561 tagttgaagt tttacaagaa ttagctgcta gaaatttagt taattctatg cacgatgttt
    13621 ctgatggtgg tttatatatt gctctgttag agtcgtcaat gcctaataat ttaggttttg
    13681 atattactac tccagcagaa aacagaacag atgcattttt gtttggcgaa gcacaaggaa
    13741 gagttgttgt ttctgtttct acagaaaaag aagcagattt tgtggatttc ttgttagaga
    13801 agaaatttcc ttttaatagt ttaggtcatg ttaccaaagg cgaattaagg attgacgata
    13861 cttcgtttgg gtttattaca gacgaaaaag aaaaatacga taatgctatt ggtaaaaaaa
    13921 tggacgcata gaagaaaatg atttctgtaa ataacttaac tatccggttt ggaaattttg
    13981 aacttttttc tgatatagga tttttaatta ataaaagaga tagaattggt ttagtaggga
    14041 aaaacggcgc aggaaaatct actcttttaa aagtaattaa tggagacata tccccaaacg
    14101 aaggaaatat ttctgttcct acagatataa aaattgggta tttaccacaa caaatgattt
    14161 tagaaaatgc taagctttct gtctttgaag aagcacgaac agcatttcaa aatgttttag
    14221 aaattcaaac agaaattgat aaactaaatg tccaaattgc taattatcac gactataccg
    14281 aagatgaata ttcaaaactt attgagaaat taactcattt gtcagaacat ctcaacatta
    14341 ttggtgctga taatgttgag caaaatattg aaaaagtttt aaaaggatta ggcttttcgc
    14401 aaacagattt tcaaagaaaa atttcggaat ttagtggcgg ttggaaaatg agagttgaac
    14461 ttgctaaaat tttacttcaa aatcctgatg ttattttatt agacgagcct acaaatcatc
    14521 tcgatataga agcaatacag tggttagaaa gttttttgga aacttatggt ggtgctgttc
    14581 ttgtaatttc tcacgataga gctttcttgg ataatgttac aaatagaact attgaaatta
    14641 atctaggaaa aatttatgat tataaagttt cttattcaaa atttaaagta ttacaacaag
    14701 aaagactggc acagcaaatt gcagcttatg aaaatcaaca aaaactaatt gaagataccg
    14761 agagatttat agaaagattt agatataaag ccacaaaagc aaatcaagtt cagtctagaa
    14821 ttaaacaatt agaaaaatta gatagattgg aaatcgatga acttgataat gctgctatga
    14881 atataaaatt tccgccagca cctcgctcag gagatattgt tgtaaaaatt gatgaactta
    14941 gccaaactta tgatggaaag aaatatattc ttaataaaat agatttaatt gttgagcgtg
    15001 gcgaaaaaat tgcttttgtt ggtaggaatg gagaaggaaa aacaactttg tcaaaagtga
    15061 taattggaga actggaatat tctggcgaat taaaaatagg acataatgta aaaataggat
    15121 attttgctca aaatcaagac gaacttttag atccagaact tactgttttt gagacacttg
    15181 acagaattgc tgttggagat attagaacta aactaagaga tattctcggc tcgtttttat
    15241 tttcaggcga agatattgat aaaaaagtaa aagttctttc aggtggcgaa cactcgcgat
    15301 tatcattagc gaaattactt ttagaacctt acaacttatt ggtgctagac gaaccaacaa
    15361 accacttaga tatgaggtca aaagatattt tgaaacaagc acttcttaaa tttgatggta
    15421 gtcttataat tgtttctcac gatagatatt ttttagatgg cttagttgat aaggtttatg
    15481 aatttaaaaa ccataaaatc agacagcata taggcggaat ttatgatttt ttaagaaaaa
    15541 agaaaatcga aaacttgaaa gaacttgaac aaaaagaaaa agttcaagct agccaaacaa
    15601 aagaaaaagt ttctaaaaat aaaattttat atttagaaag aaaagaaata gaaaggcaac
    15661 gcagaagcct tcaaaataaa gtagaaaaag ccgaaaaaga aattgaaaga ttggaaaaag
    15721 aaatagaaac tttacataat cttttgtcta atcccgaaaa aatagatgct gaagaagcaa
    15781 atgcagtttt tgaaaaattt tcaaaattaa attctgagct tgaacatcaa atggacttgt
    15841 gggaaaaagc tggcaacgaa cttcatgagt ttgaagaaaa taattcttaa aataattgat
    15901 aaatatttat ttattcgcta gtaattatta tatttgtagt gaaaaaatca agaaatggag
    15961 aaagtaaagt tagaagtttt gggtttgtca tacagtcagg ctcaggcagg agcttacgca
    16021 ttaattttgt ctgtagaaaa tagcgaagaa agaattccaa ttattattgg cggtttcgaa
    16081 gctcaaagca tagctatagc acttgaaaaa ttaagcccac cacgaccact aactcacgat
    16141 ttatttgttt cttttgccga agaatttgaa ataaacttaa aagaagttgt tatttataaa
    16201 ttcgaagaag gaatatttta ttcaaaaatt tattttgaaa aagacggaga aattaaagaa
    16261 atagactctc gaacatcaga tgctgttgca atagctctta gatttcacag ccctatttat
    16321 actactttag atatagttca aaaaacagct attattttaa attttaccga agatgacgag
    16381 cccccacgta atcaagcaaa acctgcaaaa ccaataagaa aagaagatgc aagcgaatat
    16441 ggttctatga gcgtaagtca attaaaaagt ctaatggaaa aagcaatagc agatgaaaat
    16501 tatgagcgtg catctttgat tagagatgaa cttaaaaaaa gaaatgttga tttttaatta
    16561 aaaactaatt tatgaaacaa tatcttcagt tgttacaaca tgtaaaggac aacggaatta
    16621 taaaaaccga ccgtacagga acaggtacaa aaagtgtttt tggatatcaa atgcgtttcg
    16681 atttatctga aggttttcct tgcgttacaa caaaaaaatt acatttgaaa tcaataattc
    16741 atgagctttt atggttttta aaaggagata caaatataaa atatttgaac gacaatggcg
    16801 ttagaatttg gaacgagtgg gctgacgaaa atggagattt aggacatgtt tatggttatc
    16861 aatggagatc ttggcaaaca gcaaatggag aaagtataga ccaaatcaca aaattaattc
    16921 atgatttaaa aaacaatccc gattctcgcc gtcatattgt gagtgcttgg aatgttgctg
    16981 atattgataa tatggctctt ccaccttgtc atactttttt tcagttttat gtagctgata
    17041 ataaactttc ttgtcaactt tatcaacgta gtgccgattt gtttttagga gtacctttta
    17101 atattgcttc gtatgcactt ctaacattga tggtagctca agtttgcgga tataaacccg
    17161 gcgattttgt acatactttc ggagatgctc atatttattt gaatcatatg gaacaaattg
    17221 agcttcagtt aagtagggag ccgagagctt tgccaacaat gaaaataaat cctgaagtaa
    17281 acgatatttt ttcttttaaa tacgaagatt ttgaattagt aaattatgat ccacatcctc
    17341 atataaaagg cgtagtttca gtttaaaaat tattttatga gtttttcaat tattgcggct
    17401 gttgccgaaa attatgcaat aggtaaagac aacgatttat tatgtcattt gcccggcgat
    17461 ttaaaaagat ttaaacaaat tacaagtgga aatactgtaa ttatgggtcg aaaaacattt
    17521 ttgtcattac caaatggagc tttaccaaat cgtagaaata ttgtaatatc tcgttcgcaa
    17581 aatttgaatt ttaaaaatgt tgaaatagtt gcttctattg gggaagccat taaaatttgt
    17641 gatgctgata aagaaaattt tataattggc ggaggagaaa tttataagca atttttacct
    17701 tttgttgata aaatttatct tacaaaaatc cacgctgtat ttgatggcga tacatttttt
    17761 cctgaattaa attgcgatga ttggaatgtg gagatagaag ctgaaaatac agaacatgag
    17821 cctaattttt cttatattaa tttgactaaa aaataatttt tatttttttt aagagaatta
    17881 actttctgtt gaaaaataat taattttaac ttcattcgtt ttataacata aaaagcaatt
    17941 ataaaaattc tatatctttg caaagattta tgaagttcgt aataagtcat aaagataaaa
    18001 actcaaaagc tcgcagaggg cttataagta ctgcacatgg tgaaatagaa acaccaatat
    18061 ttatgcctgt aggtacactt ggaacagtaa aggcggtgca tattactgaa cttaaaaacg
    18121 atattaaagc tcaaataatt ttaggaaaca cttatcattt atatttgcgt cccggtgttg
    18181 acataataca caaagcaggt ggtttgcaca aatttaattc ttgggataag cctattttaa
    18241 cagattctgg tggttttcag gtgttttcac tttctcaaaa tagaaaactt accgaagaag
    18301 gtgctacttt tagaagtcat atcgatgggt ctaaacatat ttttacacct gaaaatgttg
    18361 ttgaaattca aagaaaaata ggtgcagaca ttattatggc tttcgatgaa tgtacgcctt
    18421 atccatgtga ttattcttat gcaaaaaaat ctttagactt aactcataga tggctgctta
    18481 gaggctggaa acattttaat tctactcaag cgatttacga ttattatcaa acattttttc
    18541 ctatagttca gggttctgtt tataaagatt tacgtgaaga ttctgcaaaa tttgtttcag
    18601 atattggtgc ttgtggaaat gcaattggag gtttgtcggt aggtgaacca actcagcaaa
    18661 tgtatgatat gattgaagtt gtaaacgaaa ttttaccttt agataaacct cgctatttga
    18721 tgggagtagg aacaccacaa aatttgttag aaggaattga aagagggata gatatgtttg
    18781 attgtgtgat gcctacacga aatgcacgaa atggtatgct gtttacaatg aatggcacta
    18841 tgaatatgaa aaacgaaaaa tggaaagatg atttttcgcc attagatgaa tgtggaacaa
    18901 ctttcgtgga taaactatat tctaaagctt atgtaaggca tctgataatt tctaatgaaa
    18961 ttttgggagc tcaaataaca agtgtgcata acttgggttt ttatgtagaa ttgatgagga
    19021 tagcacgaga aaaaataact gaaggaaatt tttcttcatg gaaaaaagaa atgattgaaa
    19081 aactaaataa tagactttaa ataaattgaa aattttagat agatatataa tcaaaaaatt
    19141 tataggaaca tatttccttg caataacatt aataattctt attgcaatta tttttgatat
    19201 atccgaaaaa atggacgatt tcattgataa gcaagctcct ttaaaggcaa taatttttga
    19261 ttattatttt aattttattc catattttgc aaatttattt agtgctttgt ttgtttttat
    19321 ttccgttata tttttcactt cgcgaatggc agcaaaaact gaatttgtcg caatgtttgc
    19381 aagtggaata agtgctaaaa gattgttgct tccatatttt atagttgcct tttttattac
    19441 agcattttct tttgttcttg gcaattatgt aattccagaa gcaaataaac aaagatttga
    19501 atttcaaaat aaatatttta aaagatcgcc aggaaccatt acttcgggca tatcacatag
    19561 gcaaatatat ccaggagttt atttttatgt cggaaatttt aatagaacaa ataacattgg
    19621 actaaatatg tctttagaaa agtttgatgg tgtagtttta aaatctaaac ttactgcaga
    19681 aacaatgata tgggattcta ttagcgaatc gtggatagtg caaaattatc taataagaaa
    19741 ttttttagat ggaagagaag agctaataac aggacataga attgatactg cttttagaat
    19801 aactcctgaa gattttctac aaagaactaa tatcgtagaa gaaatgtcgg tagaagagct
    19861 taatgaatat attgcagaac aaaaactttc tggaacatct gctgtgattt ttagcgaaat
    19921 agaaaaacac aatagaatat ctacaccttt ttctgcattt attttaactt ttataggttt
    19981 tggcttatct attaaaaagc gtaggggagg tttgggtttg aatattggag tagggttgtt
    20041 gttaagcttt tcctacatac tttttatgag atttgcttca gtttttgcta taaatggata
    20101 tttttctgca gctttgtcgg cttggatacc aaatatcctg tattttttta tagctatagg
    20161 tatttatgta aaaacgctta aataaaataa gtataattaa attttgaaaa gatgagtata
    20221 aacaaaaaaa tcgaagcttt aaaccaaaaa cgcaaagaag ttatgcttgg cggtggtgag
    20281 aaagcaattg aaaaacaaaa agaatctggg aaacttacgg ctagagaaag aatagaactg
    20341 ttagttgaca aaggctcttt taatgaatat gatttgtttg ttcagcatag tgctagagaa
    20401 tttggcatgg ctgataaagt attaaatggt gacggagttg ttattggaac tggaacaatt
    20461 gacgggcgac ctatttgtgt gtttgctcaa gattttaccg ttgttggtgg ttctttgggc
    20521 tatatgcatg caatgaaaat tgtgaaaatt caagatttag cactaaaaat gagagtgcct
    20581 tgtattggaa taaatgactc aggcggagct agaattcaag aaggtgttga tgccttggcg
    20641 ggttatggcg aaatttttta tcgcaacaca caatctagtg gaattattcc acaactttct
    20701 gtaattctcg gtccttgtgc aggaggagct gtttattcac cagcattaac cgactatgtt
    20761 tttgttgttg acaatatctc aaaaatgttt attacaggtc cagaagtaat taaaacagtt
    20821 cttggcgaag aaattactca agaagatctt ggcggagcaa gagtgcatag cgaaattaca
    20881 ggaaatgcac atttctttgc taatgacgaa aaagaatgct tttctcaaat tcgtaagttg
    20941 ttgtcattta ttcctcataa taataaagac aaaaacacag aaagaaaagt tgaaaagcct
    21001 gttgcgaata ttgacataaa caatgttgtg ccagttgatc caaccaaacc atacgatgtt
    21061 agaaatgtta ttaaagctat aaccgacaat tctgattttg ttgaagtaat ggaaaaattt
    21121 gcacaaaata tggtaattgg ctatggaaga attggcggaa aaacggttgg ttttgttgct
    21181 aatcaacctt tatatttagc aggagtgtta gatgttgatt cttctgacaa agcagctcgt
    21241 tttattagat attgcgatgc tttcgacatt cctattgtta ctttagttga ccttccagga
    21301 tatttgccgg gagttgacca agaacatgca ggagtaattc gtcatggagc aaaagttttg
    21361 tatgcatatt ctgaagctag tgttccaaaa ctaacagtta ttttgcgtaa agcttatggt
    21421 ggaggatata ttgcaatgag ttctcgccac ttgaaagcag attttgttta tgcttggccc
    21481 actgccgaaa ttgctgttat gggacctcaa ggagctgcaa atattatttt tagaaaagaa
    21541 attgccgaag cagcagatcc taacgaaatg agaaagcaaa aagtagaaga atataaggaa
    21601 agatttgcaa atccttatgt cgcagcttca aaaggttatg ttgacgaaat tattgtccca
    21661 gacgaaactc gcgaaaaaat aatccaagct cttaagttaa gtgagactaa agctaaagta
    21721 aaaaccgaca aaaaacacgg agttccacca ttctaaaaaa aagtattatg aacgaaaatc
    21781 gaaaattaaa aatgatagta gatgaggtag agtatgaaac atattctact ccatcatttg
    21841 ataaaaagca aaaatggtct cagccagacg ataaaattat tctctcaata attcctggaa
    21901 ctatagtaga aattcttgtg aaagagggcg ataaagttaa agcaggcgat gccatgcttg
    21961 ttattgaagc tatgaaaatg aataataaaa tcaatttttt aaaaggcgga attatagata
    22021 aagtcttggt aaaaccggga gatatagttt ctaagaatca agaattgata attttaaaat
    22081 aattttctat agctataaaa aaaacgagtt taccattttt gataaactcg ttttttttgt
    22141 gggaactaat ggattcgaac cattgacccc ctgcttgtaa ggcaggtgct ctgaaccagc
    22201 tgagctaagc tcccaaaatt tcaataattc ctaaaagttt aggactgcaa atataaaagg
    22261 ctttttaata tttaacaaat attttttcat atttttttaa tcgcctaagt ctttgattgt
    22321 tatgtaatta tatttgtgtt tttgtttttg aggtttgatt ttttactaaa aaaccctaaa
    22381 acattttaca aatttgtcga aatttatggt tggattgttg ttttatgctt tttgcaccat
    22441 aattacattt tacttctaat aaacattatt accaaagaaa tttttgcata ataccatttc
    22501 ctcgcttttt tacaatggta gattcaagcg gttttatgcc atgttctttt cttcttttat
    22561 caatttccat ttttttatca tctgataaat gatcgacaag agcaattcca aaataaaaat
    22621 atggttcttc ttgtttaaac ataaggcttt ggtcatacat ccaagcataa tttctaggaa
    22681 gatattctcc ttttactagt gcttctttca aaattggaag atattttgag ttctttggaa
    22741 gtcgtacatt gtggtgaaat aaaacccata catttgaata tccttcatat cctattttct
    22801 tttccgaagg gaaaccatat ttttctatta aggttagtaa gtgctcgaaa ataactgagt
    22861 ctagttcaaa taaattactt ggtagttttg atttgtcaat gttataattt cctttacacg
    22921 acttattgtt ccgaataatg ttttgatcaa tataataaag gctatcaatt tcttttttat
    22981 aattcaaatt aatagacaat aaatgttgct tctggaaaat tgagatacta tcacatagta
    23041 ttttgtattt ttctgttttc ctaaattttt taaagtcttt tgttttaaga aagactgggt
    23101 ctagtccatt taatattgcg ttttttgcat ataaatatgt tttttcaata ttcccaatat
    23161 gagcagcaca taaactagca cttttgtatt gattagcatg cacataatca acaagtgaaa
    23221 aagctttatt gaagttcttt aacgcttctt cgtaattttt gagatagatt tgtttgtctc
    23281 cctcattaca aaaattataa tattcaatat aatcttgtcc tattaggaga ttgaaattaa
    23341 gcaatgcaag tattgcaaaa aatatacttc tcattttttt ttaatattat ttcttaatta
    23401 ttatgcccca taacgcatat ttcacacacc aaatatacaa cattaataaa tataaacaaa
    23461 taatatttgt atcaaaaaag ttggcaaatt tgttaaaatg ccaacttttt taaaatatta
    23521 ttttattcaa ggtgtttcag aaatgttcaa atgcgtttga gttaaaagtt cttaaagttt
    23581 ttaaagttca taaagttgaa cttgtgctat gcgaagcgat agacgtgtca gctactttct
    23641 actttacctt cggtgagctc gcaagctcgg ttcaactttt aacttttaac tgttttttag
    23701 tctttgatac aacgtacaga gaaaccattt tccttactat aatacttaaa cgcattgcta
    23761 ttattgctgt ctaagtatct gttccaagca tagtcgctac tatattcggt tgcactatac
    23821 caaaaaccat aagagttaag atagctgaaa acgccgctgt attctctgta tcctcctggt
    23881 agagcgttaa aaccagattc atttgtagct ccttcgtttg gaatattcca gtaaatgcta
    23941 tctgttgatt ttagttttcc tccagcaaca gtttcgccac ctaaaaactc tattaattgt
    24001 tcccattcgc tattagttgg taaatgccaa ccttctgggc aagcagataa tgctgctttc
    24061 caattatata gaactccgta tgttttgtat gtgtcggttg ctttagcttc ttcaacatta
    24121 ttaccttcat agtcatatac ataataatga ggctcagtat aagaacctgt agtaggagca
    24181 tcaacacttg gtaaatattt taaattttct gccatccaaa tttgttcgcc tatttttaca
    24241 gttttataag tattcccatc acgctcatcg gtaaaactgt cgtcagattt tttgctttta
    24301 caaccaattt gaacagcaca aaaagctagc agtaatgcaa taactgggat taaaaacact
    24361 cttcttttat tcataattat tagatttaat ttatttatac aaatttaacg ttttcctaat
    24421 tttattgttt aaaaaatttt caatttttac tcataaaatg agactgtaaa aatatacaat
    24481 aaaaattaaa aaaccaaaaa atattacatt tctgatttta atttaattag attgcatttt
    24541 gtgttttatt attgaatatt ttcgccgagt gattttattt taatttttta cctttgtgca
    24601 gaaaaataaa ttaattttaa ttattaattt tttaaaacat taacaatgaa aaatcaaaca
    24661 aaacaaaaat ctaatgttgt agcaataata atgatgatta tactttttgg tatgatttca
    24721 tttgttacaa atcttgcagc tccaatgggt gtggtgctaa aagaacagtt tgaagtgtca
    24781 aattttcttg gtttattagg taatatggct aatttcgtag cttatgcagt tatgggtatt
    24841 cctggtggat ttattcttca acgctatggt tataagaaaa cggctctatt agctgtaggg
    24901 gttggctttg tgggtgttgc tgtgcaattt ttgtcaggac atgttggaga aaattctgct
    24961 tttgcagttt atcttttagg agcatttatt gcaggtttct caatgtgttt gttgaatatg
    25021 gttgtaaatc ctatgttaaa tgctttgggt ggcggtggaa ataaaggaaa tcaactaatt
    25081 caattgggtg gttcttttaa ctctttaatg gctactttta ctccaacttt tgttggtatt
    25141 ttaattggag caactgtaag agcacaaatt acagacgtat tcccagtttt atatatcgca
    25201 atgggagttt ttgcattagt gtttttagtt ttattaatgg tttcaattcc agaacctcat
    25261 acagaaaaaa tttcagaacc catgggcggt ttgctaaagg gagctttaaa attcagacat
    25321 tttgttttgg gagcaatagg aatttttatt tatgtaggcg ttgaagttgg tattccagga
    25381 acattaaact tatggctttc agacgcaaca aaccctcaag gagcagaagt tggaaaaaca
    25441 atagcaggct ttgtggtagg aacttattgg tttttaatgc ttataggtcg tttgcttgga
    25501 gcagcacttg gaagtaaaat ttcaagtaga gctatgttga cagtagcttc aagcgtgggc
    25561 ttggttttat tgcttttagg tgtgtttatg tcgcccgaaa taaaagtttc tttgccagta
    25621 cttcaacgtt cagatgcagg agctttgtcg tttggcttag caaatgtgcc aatcaatgca
    25681 atgttttttg ttttaatagg tttatgcact tcgattatgt ggggtggaat ttttaatctt
    25741 gcagttgaag gtttaggaaa atatctacaa gtagcttctg gaatttttat gactttggtt
    25801 tgtggtggag gaattcttcc agcaattcaa agttgggttg cagatatggc tggttatcaa
    25861 ttaagttatt gggtagtaat tgcagggttt gtttatctgt tattttatgc tttggtaggt
    25921 agcaaaaatg tgaataaaga aattgttgta aaataaataa aattttaaaa atggataaaa
    25981 aatatgttgt aggaattgat attggtggaa ctaatacagt ttttggaata gttgatgcta
    26041 gaggtaatgt tttagcaaaa tcatcagtaa aaacagcagt acatcaagat gttaatttat
    26101 atataaacga tatttacgaa gaatttttaa aaatagctaa agattttggc gggataggca
    26161 atattagagg aattggtata ggagctccaa atggaaatta ttatactgga aatattgaat
    26221 ttgctcctaa tttgatttgg aaaggcattg ttcctctttc aaaacttgtt agcgaaaaat
    26281 ttggtgtacc agcagttgtt actaacgatg caaatgcagc agctataggc gaaatgactt
    26341 atggagcagc acgtggaatg aaaaatttta ttattatcac tcttggaaca ggcgttggta
    26401 gcggaattgt aattgatggc aaacttgttt atggtcatga tggttttgca ggagaattag
    26461 gacatacttg ctcggttaga gaaaatggtc gtcaatgcgg ttgcggacgt aaaggttgtt
    26521 tagaaactta tgcttcagca aatgggcttg ctcgtactgc tcgcgaattt attgaaacaa
    26581 gaaaagagcc tagcttatta agaaatattc cttgcgaatc tattacttca aaagatgttt
    26641 acgaagctgc taaacaaggc gataaattag ctatagaaat ttttgaattt acaggcaaaa
    26701 ttcttggcga agctttatca gattttgttg catttagcgc tccagaagct attatattat
    26761 ttggcggatt agcaaaatca ggcgatttta ttataaaacc aacagtagaa catatggaaa
    26821 aaaatctgct tgttctatgg aaaaataaag taaaagttct tttctctgct ctaaaagaag
    26881 ctgatgctgc aattttgggt gctggtgctt tgacatggga ggtataatct cagttaaaag
    26941 gtaaaagtcg aaaggggcga attaaaagcc tgaaattcaa taaattatat agtatagtgt
    27001 agagacggtg catgtgccgt atcttaccta tacaaaccct aagtcagtta aaagcaatac
    27061 actaaaccta gttgaagtgt taaaagtggc taggagtttt acctgattat tttttcgttt
    27121 ttttgtggct taaaaattta ttatttctct atcattaaaa aagtattatt tttgtaagtt
    27181 tttacatata atattaatag agatgaaagt aaaaattgta aataaatcaa aacacgcttt
    27241 gccacagtat agcacagagt tttcggctgg tcttgatttg cgagctaatc tcgatgaggc
    27301 aatagtttta aagcctttgg agcgtaaact tatccctacg ggattgttta ttgagttgcc
    27361 agttggtttt gaggcacaaa ttcgtccaag aagtggattg gctttaaaac atggcattag
    27421 cgttttaaat acaccaggaa ctattgacgc tgattatcgt ggcgagattg gagttatatt
    27481 agtaaacctt tcggataaag tatttgttgt acaagatggt gaaagaattt gccagatggt
    27541 aatcgctaaa cacgaacaag cagaatggat agaagttgag caacttgtag aaaccgaaag
    27601 gggagcaggt gggtttggac atacaggcgt gaaatagtta gatttttatg agtaaaacaa
    27661 aatatataaa tttctttgca atacttttag tgttgttaat ctcttcttgc tctacactaa
    27721 agaaaaacga aaaacagcca gttgacccta gaataaaaaa ttttaatttt aattattatt
    27781 tcctagaggc taataaacaa aaaattttag gaaactatgg cgatgctctt gaactatatg
    27841 cacttgcttc taatcaagac aaatctgatg cagcaagcta ttacgaaatt gcaggaatct
    27901 taaatttagc tcaagactat tcaagtgctt tagaatatgc aaaaaaagct gtaaaacatg
    27961 ataaatttga aaataaatac tatttgctac tgctagcgag tatttattct aataataatt
    28021 tacacaaaga tgctattaaa gtttttgaga aattaattga aattgagcct ttaaatattg
    28081 ggtattattt tcaaatttct tcattacatc aaatgcaaaa gaaatattct gatgcaataa
    28141 aaattttaaa taaagccgaa gccacttttg gagttagtga tgttatttgc ttagagaaag
    28201 aaaatattta tataaaaatg ggcgaacccg acaaagctgt tgccgaaata aaaaaacttt
    28261 gtgatgctta ccctgaaaaa acaataaata aaattttatt ggcagaatcg tatgtaaatg
    28321 tcggaaaacg caaagaagca gaggaaattt acaatcaagt ttgtgattta gaaatggatg
    28381 atgatattgc atatttttct attgcagact tttatctaag tgttggaaaa tataataaag
    28441 cttttgaatt aattaacaaa ggcattctgt ctcaaaacgt tgaccttaac atcaaactta
    28501 atttaatact tagtttgttg aacaatatta aagttggtga tacagatata actaataaat
    28561 tgggtgagtt tatcgaagct ttagtacttc aatatcctga tgacttaatt gtaagagttt
    28621 taaaatcaga ctatttgctt tttgttggtg attttaaaaa tgctcaaaaa gaatatgagt
    28681 ttattttaga acatgaaaag actaaaatag aaatttggca acaagctatt agtatagatg
    28741 tggcattgca ggacatgaat tcactatata ctcatagtaa agaggctgtt gaattgttcc
    28801 cattggtatt agatttttat agatactata ttatttctgc ttatgctact tcaaattttc
    28861 aagatgtggt tgaggctgtg gattttgctt cgccttatgt tgaaaaaaac cacgagttat
    28921 atatagattt tctttctatg caagcagatg cttattataa gttagaagaa tttcataagt
    28981 cagattctgt ttttgattta attttatata aagattcgga aaatattcag gctttaaata
    29041 attacagtta ttttttagcc ttacgcaatg aaaatttaga taaggcttta gaaatgagca
    29101 caaaactaat gagtttggca cctaacaatc ctagttttct tgatactcat gcttgggttt
    29161 tgtacaaaca caagagatat gcagatgctt tgattttcat aaatcaagca cttattcaag
    29221 attcaacaaa tgtaacattt ttagaacata aaggcgatat tttatttaga atagataaag
    29281 ttgaccaagc tttagagttt tgggaagaag catacaaaaa caatggtgat aacgagcagt
    29341 taaaagaaaa aattaaacaa aagaaaattt tagattagtg tttaagcgtc aaaatatcat
    29401 cattattttt ttattattgc ttgttttggt atcttgcaag gcagtaagat ttataaatgg
    29461 tggtaaaggc gaaactatac gacctcatag gctatatagg cacttggaag ccaataggtt
    29521 tgattttgaa aatgtgaatt taaaattttc ggcaaatgtg gattttggcg aaagttctca
    29581 aagttttggt ggaaatatta aaataaaacg cgatagtatt atttggataa gtttgaggtc
    29641 gtttaacgta gaaggactta gagtagtgat aactaaagat agcgttaaat atataaacag
    29701 attggataat acatattatt tgggcgagtt ttcatttctg caagaaaggt ttgctatcga
    29761 tgttgattac aacgcactag aggcaatttt aactaataat tttttctttt atccttctag
    29821 tgaagacaca actaaatctg tttcagactt taagctttgc gaagatagtg tatatcattg
    29881 tatggcttct atctcgaaac gaaaatataa ccgctttttt gttgatgaaa aaggagcaga
    29941 cagaattgag agacgattag aaagagaaag ccaacaagat agtgtaaata gattttggca
    30001 acgtggcgaa tttgaagatt attattatca aattgttaag gttatgccag ggctttatag
    30061 agtaaaagaa atgtttttag aaaattatgt gcaacaacaa agcctgtcaa tacggtatga
    30121 agatcaggtt cttgaagata cgcagttttt tcctagcaaa atttttgtag aactttcttc
    30181 gtttattttt tttgtagaat taaaactaaa tattgagtcg gttggagtaa acaatacaga
    30241 tatgagtttt ccgtttaaag taacaaacaa gtataaagaa atttcaataa aatgaaaaga
    30301 ctaaaataca taatattaat ttttgtggtt ttcttgagtg cttctcatat ttttgctcaa
    30361 agtaagtcgg aattagaaaa aaagaaaaat aaaactcaac aagacattaa ttatacaaac
    30421 aaacttttga aggaaactca aaaaactcaa aaagaatctt ataataattt attattaatc
    30481 aataaaaata ttgaattaag acgatctata attaacgata ttagttatga aattaagttg
    30541 attaacggaa aaattgcaga tatagaatta attattagta taatggagga agacttagaa
    30601 aatttgaaaa cacattatgc taatatgatt agagttgctt ggaaaaatac aaataaatac
    30661 aataatataa tgtttatttt agcttctgaa gattttaatc aagcttatct tagattgaaa
    30721 tacatgcaac agttggctaa ttatagaaac aagcaattta aagctattac tgctcttact
    30781 gatattttag ctattcaaaa aactaaactt acagatttaa aaaacaaaaa caataattta
    30841 cttactgagc aaagaaatga agaaagaact cttaaaaacg accaagttaa gcaagaaagt
    30901 actattaaaa aattaaaagc tcaagaatca gatcttaaga aaaaactcca gcaacaacaa
    30961 aaacaaatgg ctgacttaca gcgtgaaatt gaaagaatta ttcgcgaaga agctaaacgc
    31021 aatactaatg taacgactgg aaaatttgag cttacacctg aagctaagtt agttggtact
    31081 aattttgaaa aaaataaagg aaaactacct tggcccgtag aaagaggtgt gatagtaagt
    31141 ggttttggta aacaaaaaca tcctgtgctt gcaaatatag aaatagataa tagaggcata
    31201 gatatttcaa caactgttgg agcatctatt agagcagttt ttgatggtga tgtgaaacag
    31261 gtgcttgcaa tgccaggtat gcaaaatgtc gttattataa tgcatggaga atatatgtcg
    31321 gtttattcgc acttagaaac tgttttagtt agcaaaggcg ataaagtttt agcaaaacaa
    31381 gttataggta aagtttatac agataaaaca gaaaataaaa ctattttaca ttttgaagtg
    31441 tggaaatcta ccgcagctgt aaatcctact tattggctat caaaataaaa tgactgataa
    31501 ttttaaaata ttactagata aactaaatgt ctttattaag aagtattata taaataaact
    31561 tctaaaagga ttgataatat ccttgtcgat ttatgtcgct tggtatttaa tagttgtggt
    31621 agccgaatat tttggacgtt tttctagtag ctttagaaca gttttattta ttgttacact
    31681 tgcaagtttt gcttttattc taattaagat gataataatt cctattttta atttacttaa
    31741 aattgggaaa actattagct ataaagatgc aagtaaaatt attggaagtc attttccaga
    31801 agttgccgac aaattacaaa acacattaga attaaaagaa ttagtaaaaa caaatccaga
    31861 atttagcgat gttttaattg caagtataaa tcaacgtata gaaaaactaa aacctattcc
    31921 atttttatcg gcagtaagtt ttaaatcaaa tttaaaatat ttaaaatatt taggcataat
    31981 ctttttagtt gctatgttaa tattcttatt ctggcccaga atgtatagcg aggcaactga
    32041 aagaataatt aattttgata aacattatat tgagccagca ccttttactt tcgaagtctt
    32101 gaacgatagc ttattggtta aaaaaggtaa agattttgtt ttaaaagtta gtgcaaaagg
    32161 aaaatattcg ccagaccaag ttactttaaa ctatatggga aatagctttt acatggaaaa
    32221 agaaagtgta ggaatattta catataagtt taaaaatttg aataatgata ttaaatttaa
    32281 tttaagtgca gttgatgtgg tgtcgcaacc atacgaaata aaagtgttgc caagtcctat
    32341 tattgttgat tttaaagcta ttgtaaaagc acctgcttat acaaatattg aagaaaaagt
    32401 ttataataat tcaggcgact taactctacc tattggttca gaggtagttt ggaattttaa
    32461 tacttcaaat cttttatctt tgcatttagt gtttgatact gtgcaagtta aggcgactaa
    32521 gacaaatgag gtttattctg taaaacgcag aattttgcga actggaacat attctattgt
    32581 tttagctaac gaatatttta atgaaaatac cggtgtaaat tatcaactga atgctattcc
    32641 cgacttgtat ccaagcattg atgttaaaaa agttgttgac tccactcatt tgagcattgt
    32701 ttattttaat ggttttattg atgatgatta tggattttct tctttgagtt tttattgcaa
    32761 acccggcgag aaaagcgatt cgttgataag gataaatatc ccatttgcaa aatctataag
    32821 ttctcaagat ttttactttg ctttcgactt ttctaaaatt gatgttgaag gtcaaacaat
    32881 ttcttattat ttcgaagttg ccgataatga tggcgttaat ggagctaaaa aaactcgtac
    32941 tcaaaaaatg gactttgtaa ttcctagtgc agaagacttg caacaaatga catctaaagc
    33001 aaatgaggaa acagaatcta aaattgattt agcaaaagat ataaatagtc aattgcgtaa
    33061 aaatattgaa gaattacaga aaaaattgtt aagcgaacag ctcacacctt ttgaaagaaa
    33121 tcagatgctt cagcaaatta tggacaatca aagtgatttg gaaaatttga tgaaagatat
    33181 taaaaaggag caaagtcaag ttcagcagta taaagatcaa ttttctaaaa atgaggaatt
    33241 gcttaaaaaa caacaagaaa tcaatgatct tttcgactct ttaatggacg atgagatgaa
    33301 aaagatgatg gaagagcttc agaaattgat ggaagaattt aaaagcgatg atttctttaa
    33361 actcgcagat caaatgaagt ttgattatga agagttagaa aaacaaatgg ataatacttt
    33421 agagttgctg aaaaaaacag aaatagaaga aaggttgaaa aacactgcat ctcaattaga
    33481 ggaattatct aaagaacacg aaaaattatc agaagaaacc acagataaaa aatctgacaa
    33541 acaagatttg ttgcaaaagc aaaaagaaca cgaagcagct tttgacaaga taaaggaaga
    33601 ttataaagaa attttggaaa aaaattcaga gcttaaagaa cctatggatt tagagtcttt
    33661 tgaggaagaa atgcaagaaa tttccgaagg acttgagcaa tcagagagtg aaatgaaaga
    33721 cgaaaaaatg tcaaaggctt caaaatctca aaaacaaaat tctgaaaaaa tgcaagagct
    33781 ttcgcaaaaa atggaaagtg ctatgcaaga gcaagaacaa gaagaaatgt cagaaaatat
    33841 tgaggatata cgtcaggttt tagaaaattt aataacattt tcgtttaatc aggaagaaat
    33901 tttaactgaa caaaaaggtt taagtccacg cgacccaaga tatagagagt ttattttaaa
    33961 acaaaaaaac attgaagatg attttaaaat tattcgcgac tcgttaaatg ctatgtcggg
    34021 gcgaatacct caactaggtc caatggtaga aaaagaaata agaactattc acaaaaatct
    34081 tagtacgatt atggacgaga ttggcgaaaa tagacgttat ttagttgaag catcacaaca
    34141 attagtaatg acttcggcaa ataatttggc tttacttctt ttagagatga tggaacaaat
    34201 gcaaaatcaa atggcaaatt ctaaaagtgg tgatggacaa tgtaataatt gtaaaaatca
    34261 aggcgatggc agtccgaaag gaaaaatgag agatttgcaa caaggcatga agcaacaaat
    34321 gcaagatatg attgatatga tgaaagacgg gaatatgaaa aaaggtggag ctaagcaaag
    34381 cgaacaatta gcaaaaatgc ttatgcaaca agaaatgatg caacaaatgc ttaatgatgt
    34441 gatgaactcg ggattaagtc cagaatctgc taaaattttg caagaaatta atagaatgat
    34501 ggatcaaaat ctatcagata ttattaatag aaatataaat cctacaacta tacaaagaaa
    34561 tgagcaaatt ttaaccagac ttttacaagc cgaaaatagc gagcgagaaa gagaaataga
    34621 taaaaaacga aaatcaaacg aagcaacaaa ctataaactc agcaatccag acgaaatttt
    34681 taaagaaaaa gaaaaggaaa tcagatttaa tgagttattg caaaaatcta atttaaaatt
    34741 aagaactttt tataatgata aatataaaga gtatttaaag aaattaaatc aagatcaata
    34801 atggcagtaa aattttttgc agaagacgtt gaaaaacccg cattgaaata tagaagcatt
    34861 aaacaatgga tagcttcaga aattgaaaaa gaaaattttg atataggaaa tatcaactac
    34921 attttttgct cagacgaata tttgttgaaa atgaatatca agcatttaga acacgattat
    34981 tataccgata taattagttt tgattattgc gatggtaatt taatttctgg cgatttgttt
    35041 attagcttag atagaattaa agaaaatgct gtgctttatg gtacaaatga atctgagatt
    35101 tatagagtta tgatacatgg cattttgcat ttgtgtggct ataaagacga tacagaagaa
    35161 gaaagcgaaa acatgagaca acgcgaaaat gaggctcttt taagattaga tttaatataa
    35221 tcattgactt tttttcatta tcttaaattt attagcttcc gaatagtagt aattagcttt
    35281 ttcaaaattg ttgttttctt taaaataatt gctcaaagtc attccaatat taaagttagc
    35341 tttatttttt tcaaaggcaa tttcccaatt ttcaatagct tttgcagtat ttccacttaa
    35401 aagataataa ttgcctttgt caacataatt ggcatcaagc tcaggattta agtgtatttt
    35461 aaaatcaata gttttaatta tgttttcata atctttagat gtttcaaaat gtttgataat
    35521 atcatttagc gaagtggact cattgtttgg cttattataa aactcaatta cttttgctat
    35581 atagttaaag tcctttatgt ttgcataata agtcatcatg tcgaaaagac ctttggcgtt
    35641 caagttttca ttttttagat attctaagga tttaaccaag ttttcgtaat cgtgagttat
    35701 cgcaaaatta ttgataatat ctagcaaaac agttagattt aggttttcta tttttatatt
    35761 attcaaagct ttaatataat tttcgttaca tttagtggtg tcttttaatt caaaatatga
    35821 tttacccatg aaaaggtaag ctttgtccca ttctttatct ttataattat gatatttgct
    35881 tatgtaaacg ttaagatctt gaatgctttg ttcatagttg tttaaatgat aatttactat
    35941 acctctattc agataaactg cagctattgt agagtcggct tgaatacatc ttgtaaattg
    36001 ttgttcggct aattcgtagt ttttgtaaat aatcatattc atataagcca agttatgcca
    36061 tgggttggag taagaagagt ctatttcaat agctttttta aaatgagttt ctgcaacttt
    36121 aatttgattt gcaaaactat taatgctttg tttagttttt attgctgcgt aaacttcgct
    36181 tattaatatg tttccatata aattgtttgc tttggctgaa ttttctaaat atttaatatc
    36241 atgagaaaat aaactacgtc tatctttcca gtctttattt ctattaatag taattattga
    36301 atttggtaaa ataaatacta atgccaatgc aaatattaga tttttatctg tcttttttag
    36361 agataaattt agatttattt ttgtgatttt aaataataaa aatcctataa ttattgaaaa
    36421 tccaagactt ggtgtaaaca acaaacgctc tgctataatt cctgttagtg ggaaaaaagt
    36481 attggctaat ggaaaaatag aaatcagtaa aaacaaatag ccaaaagaca tcacatttct
    36541 tttattgaaa ttttttaaag cccaaattaa taatacgata ttaattacta aagaaagtaa
    36601 cacaatagga ttgctcatat caactatagg aatcatttta tatccatagt aaaatcttag
    36661 cggatatggt attattagta atttaaagta gtaaatcaag ctgtataatg ccaaagccat
    36721 tttgtcgaaa atacttgctc cgaagccaaa aataggattt tgccacataa aaactggagc
    36781 atttacttcg ggaagagatt ttttaggaat gtaatatgtg ataaatagtg ctactcctgc
    36841 aataagcaaa aatgctaatg ttgaaaagat gagttttttt gaaatgccat caaaaatttt
    36901 cactttagat ttttgagtat attcttcttt atctttaatt tttagagtaa aaagaggaat
    36961 aaaaaacatt aataagcctg ctaaatctga tttagtgaac aataataaaa ctaaagtaat
    37021 aacattcgct atccaaagca aaatattgcg aacattaaat ttaacagggc agtttatttt
    37081 gcgtctataa tataataggt ataggtagca aatgaaaaat atgccccaaa ccacatactc
    37141 attaatatta atgtataaat tgtgaagttg aacagattta ttgctagtta ttttaaactc
    37201 tggagacagt aacaaaaata gaaaaataaa aactaagaat gtgtaaacaa taattttgtt
    37261 gtgctttttg tttgggctaa acttaggaga agtgaattta tagttaggta agatttttat
    37321 ttctgttttg aaaaaaatga ttgtcagtgg aatcaataca ataaagacca atgcagactc
    37381 tttggctaaa aaagctaaaa taagagcaaa aattgaaaat agcaaatgct ggattttttt
    37441 attgtcgaaa tattttatgg cgcatatact agacattatg ccaaatataa aacttagaat
    37501 ttcctctcta ttttttaaac ttagtacaac ttctgtatgt atggcatgcg ttgcaaacaa
    37561 tagaactatt aaaaaagata aaaatgagta gttgtcggga tatttttctt taaaaattaa
    37621 gtttaaaaag taaaatagca aagcacatgc aatagcataa tataaaatat taaaaaagtg
    37681 tgcagaatgt ggattgctgc caaaaaattg atattcaatt gcgaaaacag ctattgttaa
    37741 aggtctatag ccaaaatatt gttcgtcttt tgtattatat cttgaagaaa aaatttcagg
    37801 aatccctttt attccttttt gcacaagttc atgattttca tacacgtaat cgtcgtcaat
    37861 gttaaagcca ttgtttattg acaatccgta tagcaaaaaa gatagtgcaa ttagaatcaa
    37921 atattctttg aaatgcttaa tatttgttct tttttgattt tttacgattt tttctttcac
    37981 gttttttaat tttttgttgg taaaaataag aaaaatttat tgttattttt gtttttttga
    38041 gattatttta ttattatttt tgtaaaaata atttactaaa atgttgtaat acagttgatt
    38101 tgataaagga tgtaaaaata attaaaaaaa aagtcaaaaa agttttggta gtaataaaaa
    38161 agactttatc tttgcagtcc caaaacgata agaaaatatt aaata
//
LOCUS       DAOPNX010000016        34520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_1651_length_34520_cov_6.467663, whole genome shotgun sequence.
ACCESSION   DAOPNX010000016 DAOPNX010000000
VERSION     DAOPNX010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 34520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34520
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1651_length_34520_cov_6.467663"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="PKN53_03060"
     CDS             complement(<1..1326)
                     /locus_tag="PKN53_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOD87964.1"
                     /translation="MKVKYILISFLVLFFVNEIRAYDIKIEIENCPEYYVFFGKHRGP
                     DFEIIDSLPVKNNFVNFKSPEKLEHGVYFVVIPPQTRFDFIIDEVQDFSIKTNMYNIL
                     GDMQIKGDEEYSLFVNMQKEIAKINKIRTQLEVQSEFYKNFQPDTIPFIKKEIDSLNL
                     LQQNVYKKYRSQTNPEKFLNKILAILEGFEVPDSISVLVYTNPQKHFDYYKTHWLDRV
                     DFSESGLLNTPAFVFHKLLEDYCFYFLDTKANNIETAFADVDTLVHKTKNDINFNQYI
                     LSYLISRYENPKDLRLERLLVYVYRTYFLANPPQWLDERAFSIMKFRIEAIQYNLIGS
                     VAKDLNLPDINGNFHSIHKMPNKYKLLLFWEPDCDICNETAFILQSSYYKLRELDVEI
                     YAVLINSENDEWQEFIEENAIEWINVHDPKNETNFEKYYGTYKTPRLFLL"
     gene            complement(1342..>1929)
                     /locus_tag="PKN53_03065"
     CDS             complement(1342..>1929)
                     /locus_tag="PKN53_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOD87965.1"
                     /translation="YFESSSIMGYDAVFVAIAEDWYMSGKAKWASEEYLEKIIERVNK
                     ITPTKLGNTATDLVRMQTIDDKYISLHQVDADYTVLVFYEPHCGHCKKEVPKLMEHYR
                     DSLKDLNVKIFALYTQYDKEEWKTFVEEKNLYEDGWYNVWDGPYPHSKFRDFYDIYST
                     PVIYVLDREKIIIGKRIGVDNIKSLIEFVKKKSEQ"
     assembly_gap    1930..2029
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2030..2930)
                     /locus_tag="PKN53_03070"
     CDS             complement(<2030..2930)
                     /locus_tag="PKN53_03070"
                     /inference="COORDINATES: protein motif:HMM:NF025647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HOD87966.1"
                     /translation="MKKIGLLVLGFFISFLLSAQNYEIKIKINGVSDTVAYLGHHFGE
                     KKYVVDTAKVDSKGNVVFAGDKKLERGIYIVVLPSRGMTYFELLIGDNRKFSLETDTT
                     NFLQTMKVKGDKENQAFMDYQRKMVDIQNRRTDLMDIYEAAKDNPDEQKKISEEFTKL
                     NDERISFMNDLIKKNPNTLLSKILLSMIDPVVPESPKDEEGNEIDPYFAYKYYKAHYW
                     DNIDWTERGLLRTPIFENKLDYYFEKMVVPVADSMIPESRHLINTAYEAGDSLMFQYM
                     ASHLLNYFESSSIMGYDAVFVAIA"
     gene            complement(3040..5634)
                     /locus_tag="PKN53_03075"
     CDS             complement(3040..5634)
                     /locus_tag="PKN53_03075"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOD87967.1"
                     /translation="MLILFLTIIVSFVSSINAHNNLYTTKYELKINAYQMVDDTIKPQ
                     TKVDSVSIKKSSMSFDYPVYYDAEDSLLFDFIAEKAYLYGKAVVIYENINMSAEYIQM
                     DFDKNEVLAHGLTDSAGNIYGKPIFKEGEEEFIADTVRYNFKTKKGIIKEVMTEFEGA
                     FLHGSKTKMQENKDIHMIDGKFTTCDLAHPHYYFAISKAKVIPDDKLVSGPAYLVIED
                     VPTPLGIPFGFFPNKKGSTSGILIPEFGNEDNRGMFLRNGGYYWAVNDYLDLTFLGDI
                     YTKGSWGVSLLSNYKVRYKFSGHLNFKYNRNMTGYKDVNRQVTDLFAVNWKFTQDPKA
                     LPNTTFAADVNMSSANFDKYNSYSLNNYMSSNKQSSISISKIFPNSPFSLTAAIRHNQ
                     NNNTKAITLTAPDLSLNMNRIYPFKRKVKVGESKIYEKIGIAYSMNLTNKINTTDTTL
                     FDINFDDMINGISHKIPISTSAKFLKYTTLTPAFNYNERWYFKNMYKFWDFENENDTI
                     GFLNSNYNSGFSRVWDYSASLNWSTQLYGTFNFKRGKLKALRHVLSPAISFSYAPDFG
                     KPKYKYYDDYYRIQYNGTTGQYDTVSYYYSRFEGLPYGAPARGGAGSLNLNLGNNLEM
                     KLRNSKDTTKTDQKVKLLESFNLSSSYNIMTDSLNWSPIAVSARTRAKLIDLNFNANL
                     DPYAVVENAYGSGGVRINKLAKDINKQILRLTTLNFSASISLNSKVGESKAPVSQDKD
                     PYNVLYGYPNDYVDFNIPWNVRISYSLRYAKPYFESKTTQTLNVFGDFNLTPKWKISF
                     NTGYDFENNKVTPTTIDIYRDLHCWEASLHLVPFGQHKSYMFQINVKADMLKDLKLAK
                     RRSWFDNF"
     gene            5735..6949
                     /locus_tag="PKN53_03080"
     CDS             5735..6949
                     /locus_tag="PKN53_03080"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOD87968.1"
                     /translation="MIKNKCFFIFSLVFFLFGTGKIANSQSFAIKTVVIDAGHGGKDP
                     GAVGKISKEKDLTLKMALMFGEEIKKAFPSIKVIYTRDTDKYLALHERANIANKNHAD
                     LFISIHINASDSKSANGTETFVMGIDKSGANLNVVKLENSVILQEENYQENYGGFDPN
                     DPESHIIFSLYQNVNLNQSLVMAQAIQDKFTKDVKRFSRGVKQAPFLVLWKTSMPSVL
                     VELGFISNLEEEKYLNSDAGQKALVKAMFDAFKVYKKSYEDESVEPANSKNNEPEIKT
                     EVNKEENKIEIKEETKVEQEVKQTENQKITNEETVAKPAESEIIFKIQIMTSAKKIEI
                     KPKNFKSYQNVDVYLQDGMYKYTIGRDSDYDKIVELHKKVKLDYSDSFIVAFKNGERI
                     PLNEARSQQKNK"
     gene            6946..8331
                     /locus_tag="PKN53_03085"
     CDS             6946..8331
                     /locus_tag="PKN53_03085"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOD87969.1"
                     /translation="MNKKKKYIFYSFVAIAILAVTYWGFNFLKGVSLFNKVNSYYIYY
                     DRIEGLNESSYVTVNGYKVGQVGEIKLLPAENYKLLVRIDITNEFAIPDSTIARIYSM
                     DLMGTKGIELEFGNKTTYIAPEGFLIGQVEQSLKDQVNMQMLPVKQQAEQLMAELTHA
                     ITVITYIFNEDTRANLEKSFASIKNTLVYIESSAISVDGMLKEESTKIARTLSNLEFI
                     TAMLKDNSENMENVFKNLSSFSDTLVAIDISKTMAQANDAIQNFNQILDRANRGEGSL
                     GKLLNDDNFAQQIEDAATNLKSLLFDIQYNPKKYVNFSLMNIGRTVNVADPSELSKRD
                     KRVLERQLEKSEKEVQKNYEKEQKRQEKENKKDDLSNYQGKVIFMIQIRSAVDKIDLN
                     SDELKGYTDVIEIKSGAYYKYLIFPHESTSQTDYYLNLAKEDFSDAFPVALVGDQVVS
                     YAKGIAIVAGE"
     gene            8752..9318
                     /locus_tag="PKN53_03090"
     CDS             8752..9318
                     /locus_tag="PKN53_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOD87970.1"
                     /translation="MDIYELIKSRRSPRGYYDKGIKPEDLEKMFDAARWAASAYNFQP
                     WRFIYADKEKDPELYNQILELLVEFNQAWAKAAPVLIVTLVNTRNPKTGDTLTHAKYD
                     LGLAVGNMTLMAESMGISLRNMSGFDANKAREVLNIPDYMEAVTIIAVGYGGLPGNLP
                     LDIYKMEIMPRERMELNEIVFNKPFDKE"
     gene            complement(9404..9835)
                     /locus_tag="PKN53_03095"
     CDS             complement(9404..9835)
                     /locus_tag="PKN53_03095"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HOD87971.1"
                     /translation="MKKISFILVFLLSFGIIAHSQSITFNITGIQKAIGRIHLGFFTN
                     EKDYKIEKPTINKYISKKDLKNGKITVRFDDIPAGTYGVCLLDDENENGKMDYSFFIP
                     QEGFGFSNYYHKGMSKPKFDSFKFDLDSGVHKVVEIKIRYM"
     gene            complement(9984..10328)
                     /gene="rplT"
                     /locus_tag="PKN53_03100"
     CDS             complement(9984..10328)
                     /gene="rplT"
                     /locus_tag="PKN53_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005469617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOD87972.1"
                     /translation="MARSVNSVASRNRRRKVLKQTKGYFGARGNVWTVAKNAYEKGLT
                     YAYRDRKTKKRNFRALWIQRINAGARLNGMSYSQFMGKIHANKIELNRKVLADLAMNH
                     PKAFEAICKKLK"
     gene            complement(10408..10605)
                     /gene="rpmI"
                     /locus_tag="PKN53_03105"
     CDS             complement(10408..10605)
                     /gene="rpmI"
                     /locus_tag="PKN53_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOD87973.1"
                     /translation="MPKMKTKSGAKKRFSLTGTGKIKRKHAYKSHILTKKTIKRKRAL
                     THTTTVADPDKKNILLCLGMK"
     gene            complement(10631..11185)
                     /gene="infC"
                     /locus_tag="PKN53_03110"
     CDS             complement(10631..11185)
                     /gene="infC"
                     /locus_tag="PKN53_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOD87974.1"
                     /translation="MEKRSNFSQNKKQGEHRINNYIRAPFVRLVGDNIEKQGIVSLRE
                     ALEVADNLGLDLVEISPNAEPPVCKVIDYQKFLYQQRKRQKEIQSKAVKVTIKDIRFG
                     PNTDEHDYNFKLKHAQKFLEEGNKVRAFVFFKGRSILYKEQGEILLLKLAQDLEEYAK
                     VDQLPKLEGKRMQILLSPKTSKKK"
     gene            complement(11203..13143)
                     /gene="thrS"
                     /locus_tag="PKN53_03115"
     CDS             complement(11203..13143)
                     /gene="thrS"
                     /locus_tag="PKN53_03115"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOD87975.1"
                     /translation="MIKITLPDGSTREFEKGVNGLQIAENISPRLAKEVLAISINKEL
                     SDLTRPIYEDAKIVLHKFEDEEGKHAFWHTSAHVLAEAIESLFPGTKFGIGPAIENGF
                     YYDIELPDGQALSENDFPAIDKKMRELCQQNNPVVRREVSKAEALSTFEKKGDNYKTE
                     LISELEDGTISFYEQGNFTDLCRGPHLPSTGYIKAIQVLSLAGAYWRGDETRKQLTRV
                     YAISFPKQKMLEEYLTMLEEAKKRDHRKIGRELELFHFSQKVGMGMALWLPKGTILRD
                     RLESWLTEELKKRNYEMVKTPHIGHIDLYKTSGHYDKYGKDSFQPIQTPAEGEAFMLK
                     PMNCPHHVEIFKARQYSYRDLPYRMAEFGTVYRYEQHGELHGLTRVRSFTQDDAHVFC
                     RPDQVKDEFKKIIELVMYVFKSLDFNEYKAQISLRDPDDKTKYIGSDENWEKAEQAIQ
                     EAAQEMGLKPTVELGEAAFYGPKLDFMVKDALGRTWQLGTIQVDYNLPERFELEYKGS
                     DNEMHRPIMIHRAIFGSMERFVAVLIEHTAGKFPLWLTPQQVVILPISEKFNNYSQKV
                     LDLLKNYDIRGGIDDRNEKIGRKIRDNELNRIPYLLIVGEKEVENNTISVRKQGQGDL
                     GVMGIEEFVELINKEIKEYFTN"
     gene            13290..14552
                     /locus_tag="PKN53_03120"
     CDS             13290..14552
                     /locus_tag="PKN53_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87976.1"
                     /translation="MKRNLLIFVLIFVFVNSAHFAFGQKDEKQFGVKFSGYVKNDFYY
                     DTREVVNGREGHLLLWPQSPSYDTEGRDINAHGKFHFLAIQTRLTGNITGPDALGAKT
                     SAMFEGEFFGSSDSDINGFRLRHALIKLNWEKSEILTGQTWHPMFVTENSPGTVSFNT
                     GLGFQPFARNPQIKYTFRLGNIRLAATAFTERDFQSRNLTGTPSGDYIRNAKIPALNF
                     SFNYGKNNNEAKTAFNTGFAVNYKNIRPSLTTPLGYITKENVSALSAQVYIMKKFKPL
                     SVKFAALYTENANEMFIPSGFAIYEIINLTKMEYAYLPTRNAISWIDIATTGEKWQFG
                     IFAGYNHNLGTVKNIMVSPVGLGVGIEHLYRVSPRVYYFAGKLQIGAEIEYTVAAYGD
                     GTYTTKAIPQNAEEVANLRLLLGVFYHF"
     gene            14664..14755
                     /locus_tag="PKN53_03125"
     tRNA            14664..14755
                     /locus_tag="PKN53_03125"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14697..14699,aa:Sec,seq:tca)
     gene            14933..16393
                     /locus_tag="PKN53_03130"
     CDS             14933..16393
                     /locus_tag="PKN53_03130"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOD87977.1"
                     /translation="MKYKHVLISFFSGIIGAIIAVLIIDSYKTSSDGEIKNQHSLVAD
                     YYLNKQHQLPATTVANPFLFSPSSSFDFTSAAEMSVPAVVHVKTQYSQPNYTLYDFIF
                     GTSPRYSTPVLTSGSGVIVSPDGYIVTNNHVVEDAETIEVVLNNKKSYSAKLVGRDAT
                     TDIALLKVNANNLPFLNYGDSDNIKIGEWVLAIGNPFNLTSTATAGIISAKARNISIM
                     SENMAIESFIQTDAAVNPGNSGGALVNTKGELIGINTAIASRTGSFVGYSFAIPVNIV
                     RKVVADLVEFGEVQRAYIGIDLLDIDSDLANQLSISKIEGVYVANVLANGAGDKAGLK
                     AGDIITAINDTKVNSVSEFLEYLSRFRPGDRVNLSVKGGRTTKNIKLTLQNKYGETSI
                     VKSEAIDDLGAKFEVLSDYDKARYRVKAGIRVTDISQGKFLANGIKKDFIIIRVNNEF
                     VNTVKDFQDIIKKSNGSLFIEGFYPNGSSAYYSFRL"
     gene            16882..17661
                     /locus_tag="PKN53_03135"
     CDS             16882..17661
                     /locus_tag="PKN53_03135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOD87978.1"
                     /translation="MNSSKAFIISGFVLLLMFCIVYVAYGYNSEKSDVLTQANNPKSF
                     NSFTDPRDGNVYKVVKIGEQVWMAENLRYLPSLAGSDMSRIEPCYYVYGYEGTNVEEA
                     KATKNYKTYGVLYNWNAAFYACPEGWHLPSDAEWAQLVDFIGGEEIAGGKLKEAGTAR
                     WDKPNTGAINKFGFTALPCGIYFKEFIGIGSFGLWWTATLDDNPYYDMLTHKLINDYA
                     LIRMINYDENNVDCAFTDTNSGLSVRCVKNVTVVIGFNSAN"
     gene            complement(17684..18322)
                     /locus_tag="PKN53_03140"
     CDS             complement(17684..18322)
                     /locus_tag="PKN53_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87979.1"
                     /translation="MENRFPLALRYNIKEQKFYVSKLYSFPYIEEGIYYPTSWHVDYF
                     SISKNNLPLIRYNYSSSIFEWDYINDKLITHSFKSRLIDTIMPMETPSTYHFHTLECA
                     YQNAHYDKYRELYFLEFYFNENFYINNRDLYSVIIADKDFNYLGELYCNKSWPAYYLE
                     DYILDIYPSGSSTINVDYLELVKTDEIFKSEETETVLYQGLLNSLGLCVINE"
     gene            complement(18631..18891)
                     /locus_tag="PKN53_03145"
     CDS             complement(18631..18891)
                     /locus_tag="PKN53_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87980.1"
                     /translation="MKSTVLKLMLIAILFSACCKEKPQPSVYDLKIVKSKQIELEDGD
                     IDYVFAKEQVINIDGKNIIILNVAEKSSLFTILTKVKKSMKY"
     gene            complement(19269..21275)
                     /gene="gyrB"
                     /locus_tag="PKN53_03150"
     CDS             complement(19269..21275)
                     /gene="gyrB"
                     /locus_tag="PKN53_03150"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOD87981.1"
                     /translation="MDENKEILEKNGGNDTSSHTNGKNGNGNYSADSIQVLEGLEAVR
                     KRPAMYIGDISTKGLHHLVYEVVDNSIDEALAGYCDKIKVTINEDNSIKVEDNGRGIP
                     VDLHEKEGKSALEVVMTVLHAGGKFDKDSYKVSGGLHGVGVSCVNALSSKLTATVKRD
                     GKIYFQEYSIGKPLHDVKVIGETEETGTCVEFWPDNSIFITTEYQVDILSSRLRELAF
                     LNKGIRLILEDKRQVDENGIAKSKEFYSEHGLKEFVEYLDQGREKLTENIIHISTEKN
                     DVPVEIAMQYNTSFTENIHSYVNNINTIEGGTHLTGFRRGLTRTLKSYAEESGLLSKV
                     KFEINGDDFREGLTCVISVKVQEPQFEGQTKTKLGNSEVSISVDQAVSSLLKNYLEEN
                     PKDAKAIVDKVILAATARHAARKARELVQRKNVLGGAGLPGKLADCSDRNPENCELYL
                     VEGDSAGGTAKQGRDRNFQAILPLRGKILNVEKAMPHKVFDSDEIKNIFTALGVTIGT
                     EEDSKAVNLEKLRYHKIIIMTDADVDGSHIATLIMTFFFRYMKPLIEGGYLYIATPPL
                     YLIKKGKTEQYAWSENERLDIIETLGQGKETGLHIQRYKGLGEMNAEQLWDTTMNPET
                     RTLRQVTIENAGEADHIFAMLMGDEVAPRREFIETHATYAKIDA"
     gene            complement(21498..23204)
                     /locus_tag="PKN53_03155"
     CDS             complement(21498..23204)
                     /locus_tag="PKN53_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87982.1"
                     /translation="MKTTSILIIAILFLGLNLKAQQDEKPGNLEETGIIIYTEFAPVL
                     KDANRMQFLPTIVDTVEVNPHFEYKIEPVLFKTSFKPTPITAASFSGEPLKPLYNGLI
                     KLGMGNYLSPLAEVYYNTTRQRNYSAGAFVKHHSAFGKVKNSADQKIFSGFNNNLVNA
                     FAKRFFYRSTLGADISFKSNQAYFYGYDPNIIVPTDFVKPRDREEMEMQRYNKLRANI
                     NLESNNSSERRMNFKSNLAYQYFFTQSKNTQHKIDFEADLNKLVKKNRFGMDLGFVFN
                     NDQIQDSAVFNFKETFLDLNPYFKHYSNNWQICLGVNTTGELSKQPVAYHIYPNVQFQ
                     HNISNTIIPYASFTGYVQNNTVEFLSDINPFINNFHTNKITNYAQVIDVGLKGNISRK
                     VFFHLNANYSKIDDMGFFVNDTTTMLENKFVLIYTNVERFTGYGEIALNDLFDKLSIN
                     LKGHYYHYSYIKNREKPWHMPTVDVRLNTNYKFDNKLNFGLNADFIGARYAKEYDADL
                     NIVEKKLKPIVDVSLYGEYSFASNFQAFLNLNNITGQKQYVWNNYMSHGFNFMLGLKY
                     LF"
     gene            complement(23225..26386)
                     /locus_tag="PKN53_03160"
     CDS             complement(23225..26386)
                     /locus_tag="PKN53_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD87983.1"
                     /translation="MKIRFISTALVVLISFSIQAQTTQAYNEMETKWTSAINLYEKGM
                     YVPAQKLFSEILLHDGQEYASYKDEAAFYYALSSMNLLNNDAEFQITNFIENHPESRN
                     SNNASFQMANFKFRNKDYKNAIKWYEKTDRLLLQADEKSEYFFKLGYSHFARKDFDRA
                     AKAFYESKDANNIYGPMSLYFYSHLKYINEQYQTALLGFLELEKSPSYASITPFYIIH
                     IYYLQEKYDELIEYAPKILEKESGGRAAEINKLLGSAYYRKGRYSEAIPHLNNYVNST
                     SSANDYDYYELGFAYYNTKQYDKAANSFSKIANMKDTLSQNAAYHMGDCNIKLDKKIE
                     ALRSFELASKYNYNPKIKEDALFNFAQLSFELSMSPFNEAINAFTLYIKEYPNSPRTD
                     LAYDYLIDAFLSTKNYQASIATIEKMQNRNTKIDAAYQRLTYFRGLEYFTQLKYNEAI
                     TMFDKSLDYKIYDKTIDALAHYWKAEANYRLGNVEDAITMFKDFVNTTGSVSLDEYSK
                     AHYNLGYAYFNKKEYVVANSWFRKFELQPGYANSDILNDVLNRIGDCYYIEKEYTPAA
                     EYYKKAIDIGKISPDYTLYQMALSYGGLKKPNEKIWSLRRLIQNYPESEYLSNANFEI
                     GRTFHTTLNNPDSAKFYYNRHIEKFPNSSMRKASLSSLGAIYFNERNYPKALELYKQV
                     IAEYPNTEEAKNAADMIKAVYIEQDNPDGFVDWANASGTNISEDEQDEIMWQIAKKNY
                     VDKKYNEALSSLTNYLKSFPKGKFYIEANYYKAELHFYFEEKDLALENYRVVANAPRG
                     AFTEESTIKASSILFDKEAWEEAYSLYDKLYSLSENKSTTLLASIGKLRCAYNLNNWD
                     NTIAAAMQIIDNERSNDEEKRESHFKMAKAYYNKDNLIRALNLFENIATETKSAEGAE
                     SKYRVIKIHYKMQRDSIAEELAYEFAQSNSPHAYWVAKTLICLADIYYDREDFFSAKH
                     TLQSLLNNYPIETDGIKDETSQKLANIIDEEQGKRSSEDLLNLKINLSGEEENDNTQS
                     SDNNSDNENKESEISLPEPAKIE"
     gene            26583..27284
                     /locus_tag="PKN53_03165"
     CDS             26583..27284
                     /locus_tag="PKN53_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD87984.1"
                     /translation="MEDSTYLIKTENLNVYQDENLILENVNIEIDLGEFVYFIGKVGS
                     GKSSLLKVLYAELDIKEGEAKVAGYNLNKIKNKHIPMLRRKIGMVFQDFELMADRNVF
                     KNLEFVLKATNWKSKIDIDTRINEVLESVGMLDKKISMPTKLSGGEKQSISIARALLN
                     NPPLIFADEPTGNLDPESADSLMKILHELPKQGKSVIMVTHNHAFLKKYPGRVFLFED
                     GKVTEQIEQGIDLIS"
     gene            27823..28953
                     /locus_tag="PKN53_03170"
     CDS             27823..28953
                     /locus_tag="PKN53_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD87985.1"
                     /translation="MINRILSKEIDKRLFAGKAIIIIGARQVGKTSLLHSKFDGNPNV
                     LWLEGDNIETQLLFEDFTLSFAKTLIGDKKILIIDEAQNILNIGIKLKIIIDQMKDIQ
                     VVATGSSSFDLANKINEPLTGRKWEYKLYPISFAEMVAHHGFFEEYKMLEQRLVYGYY
                     PEVVMQKNENMVEVLQGLATSYLYKDVLKWENIQKSDRMIKLLQALAFQIGGQVSYTE
                     LGKICGLDSKTIEKYILLLEQAFIIFRLNSFSRNQRNELKFSKKIYFYDNGIRNALIA
                     NFNPVNLRDDLGALWENFMISERQKKNHYSHSYANSWFWRTQTQKEIDYLEEKDGVIN
                     AFEFKWNKNKKTRMPLDFMKSYPNAIYSTITPENFYEFLSME"
     gene            complement(29087..29482)
                     /locus_tag="PKN53_03175"
     CDS             complement(29087..29482)
                     /locus_tag="PKN53_03175"
                     /inference="COORDINATES: protein motif:HMM:NF014599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HOD87986.1"
                     /translation="MTNNKMESGFRINNLILLESKFKRINNVQFEDANLDLNIDTEVS
                     VNNNIISVAETVSVKKTHNNTEQFSFLVKMVGIFECIGESQLKDYEKFGKINGAAIIF
                     PYIREHITNLSMKAELGPIILPPVNFTKN"
     gene            complement(29484..29942)
                     /locus_tag="PKN53_03180"
     CDS             complement(29484..29942)
                     /locus_tag="PKN53_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87987.1"
                     /translation="MEAKNFIYNELKVFIKKFSKTRVRYEYDQNALVHVVEVLPNEVY
                     HLDNDYICWENDIFNRFITQFPTENICFISDDALVGIENPELVLEGLEYAPFSWVATS
                     QKIKNTFSTNLNLFTINSFDNINSFENKIESGFENIEIPISQILTILKAA"
     gene            complement(29945..30214)
                     /locus_tag="PKN53_03185"
     CDS             complement(29945..30214)
                     /locus_tag="PKN53_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87988.1"
                     /translation="MKKSEDELRSNTSQSRYGSHEYLLNEVVKYIGNLQNKLSANDIR
                     NLRNNLLQLKRLRTDADYSDSNFDIKKSQNSIELSERVLPILKRY"
     gene            complement(30224..30358)
                     /locus_tag="PKN53_03190"
     CDS             complement(30224..30358)
                     /locus_tag="PKN53_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87989.1"
                     /translation="MHKSHLQNKSELLEASASLLQDNYFYPAVAHAAYYSCYQLMKHI
                     "
     gene            30587..31567
                     /locus_tag="PKN53_03195"
     CDS             30587..31567
                     /locus_tag="PKN53_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOD87990.1"
                     /translation="MNSFGRIFRISIYGESHAGELGVLIDGVPAGILLSEKDFELAIE
                     RRKPNQVGSTSRKEDDIPLIKSGVFNSYTTGAPILISFENKNIKSEDYNFNGFYRPGH
                     SDFVANKKYKSFNDFRGSGHFSGRMTLALVAAGVVANKILPDIKISASVISVAGSKDF
                     QTKIDEAEQNGDSLGAVVECRINNLPVGYGEPFFDSLESVLAHLIFSIPGAKAIEFGN
                     GIFAGSAKGSEFNDVFINENGETKTNNSGGINAGISNGNEIVFRVYFRPSASISKAQE
                     TFNFETKKMGELKIQGRHDVCYALRTPVIVESVAAIVLADFKLILSSQKL"
     gene            31564..34365
                     /locus_tag="PKN53_03200"
     CDS             31564..34365
                     /locus_tag="PKN53_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOD87991.1"
                     /translation="MKSFLFEVAQNIIQQKDDFQNICIVVPNRRTELFLKKEIVNSLN
                     ETSWLPQIITIKSIFESNSNLIVEDDLLLISKLFNVYKNCTGSNENFDNFYYWGQIIL
                     SDFDDVDKYLVDAEKLFASINDIQEIDAKFSSFDEKELEIIKRFWTNIDIDKPSSHKA
                     EFLSLWSKMYDIYSEFRKILFEQGVAYQGMAYRDLVENIHNVNLEKDKYFIVGFNALN
                     KCEIEVFKFLKTNRETAFFWDYDDYYVKNNEHEAGRFLRNNLKLFTNDLKSNNDKIKS
                     QNLDVEVISLPTSVAQVKLIEEILTEWKAQPDFDIEKVAIALGDENLLIPLMTSIPDF
                     VGDYNITMGFPLKNTKAFNFFSNLLSLRQNSRFLTDSLKFYHKDIVSILTQTYISEFF
                     SKDAQEILEYINKEKSIYVDLSSFTQNEFLAKLFDANVKDVPEILNWLSEVIADVLDL
                     VSKKEDFIFETEFLLKLSNSINLVSDCFIDGKIIIERKEILFKILLNAVKSVSLAFEG
                     EPLKGMQVLGFLETRNLDFDRVIMLSLNEGVFPKKSAAQTLIPYNLRKFYELPSIEFQ
                     DSIFAYYFYRLLQRAKDVKILYAAQGDEKISGEVSRFVTQIKYEADFDIKFINKAYKI
                     SLSNENVLNVNKTPEVIEKIQTHLQNGISPSAINSYLSCEFKYFLRYVKGLKEVDEIE
                     EKEDAALFGSLFHEIMKLLYEPYKNQVLNKELFKQIRADKNINKCKIEAIKKVFKVKS
                     EVQIAETEKKLIVDVVEKYVKRMLDFDSQSPEFSIVDLEAEYSRDIKLANDFSVKIKG
                     NIDRIDRQGKTLRVIDYKTGKVKNSFSEISELFEQDRTYELDIIMQLFLYAYILREKY
                     DYVCPSVFSLNELNKDYDGSISMKREKLTDFSDDLLQAFEENLISLFESMLEPNTIFS
                     QTTDSDNCKFCPYKGICNK"
BASE COUNT        11466 a         4984 c         5539 g        12431 t
ORIGIN      
        1 tagtaaaaat aatcgtggag ttttatatgt accgtaatat ttttcaaaat ttgtttcgtt
       61 tttagggtcg tgaacattta tccactcaat agcattttct tcaataaatt cttgccattc
      121 gtcattttca gaatttatta aaacagcata aatttccaca tctaattctc gtagtttgta
      181 ataacttgat tgcaaaataa aagcagtttc gttgcaaata tcgcagtcgg gttcccaaaa
      241 taaaagaagt ttatatttat ttggcatttt atgaatggag tgaaaattac cattaatatc
      301 aggcaggttt aaatctttag caacagaacc aattaaattg tattgtatag cttctattct
      361 aaatttcata attgaaaaag ctcgttcatc aagccattga ggaggatttg ctaaaaaata
      421 agttctgtaa acataaacca ataatctttc taaacgtaaa tcttttggat tttcgtaacg
      481 agaaattaaa tacgacaaaa tatattgatt aaaattaata tcgttttttg ttttgtgaac
      541 taatgtatca acatctgcaa aagcagtttc tatattatta gctttggtat ctaaaaagta
      601 aaaacaataa tcttcaagaa gtttgtgaaa aacaaatgca ggagtgttta ataatccaga
      661 ttctgaaaaa tcaactctat ccaaccaatg agttttgtaa taatcaaaat gtttttgagg
      721 attagtataa accaagacag aaatggaatc tggaacttca aaaccttcta aaatagcgag
      781 aattttattt agaaactttt caggatttgt ttgacttcta tattttttat aaacgttttg
      841 ttgtaagaga tttaagctat ctatttcttt ttttatgaag ggaatagtat ctggctgaaa
      901 atttttataa aattctgatt gtacttcaag ttgagtacgg attttattga ttttagcaat
      961 ttccttttgc atatttacaa aaagggaata ttcttcatca cccttaattt gcatgtcgcc
     1021 caaaatattg tacatattag ttttaatact aaaatcttga acttcatcaa taataaaatc
     1081 aaaacgagtt tgtggcggaa ttacaacaaa atacactcca tgttcaagtt tttcgggaga
     1141 tttaaaattt acaaaattgt tttttacagg aagtgagtca attatttcaa aatcaggtcc
     1201 tctatgtttg ccaaaaaaca cataatattc agggcagttt tctatttcaa tttttatgtc
     1261 gtaagctctg atttcgttta cgaaaaacaa tactaaaaaa cttattaata tgtattttac
     1321 tttcacagtt tttgttttag cttactgttc agattttttt ttcacaaatt caattaaact
     1381 tttaatatta tcaacgccta tacgttttcc aataattatt ttttctctat ctaaaacata
     1441 tataacagga gtgctataaa tatcataaaa atctctaaat ttagaatgag gataaggtcc
     1501 gtcccaaacg ttgtaccagc catcttcgta tagatttttt tcttcaacaa aagttttcca
     1561 ttcttctttg tcgtattgtg tgtaaagtgc aaaaattttc acattcaagt cttttaaact
     1621 atctctgtaa tgttccatta atttggggac ttctttttta caatgtccac aatgtggttc
     1681 gtaaaatact aagacagtat agtctgcatc aacttgatgt agtgaaatgt atttatcgtc
     1741 gatagtttgc atgcgaacaa ggtcagtagc agtgtttcca agttttgtag gtgtaatttt
     1801 atttactctt tcaattattt tctccaaata ttcttcacta gcccactttg cttttccgct
     1861 catataccaa tcttcggcaa tagcaacaaa tacggcatca tatcccatta ttgatgaaga
     1921 ttcaaaatan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ggcaatagca
     2041 acaaatacgg catcatatcc cattattgat gaagattcaa aataattcaa taaatgtgaa
     2101 gccatatatt gaaacattag ggaatcacca gcttcgtaag ctgtattaat tagatgacga
     2161 gattcaggaa tcatagaatc agcaacagga acaaccattt tttcaaaata atagtctaat
     2221 ttattttcaa aaatcggtgt tctaagtaaa cctctttctg tccaatctat attgtcccaa
     2281 tagtgagctt tgtaatattt gtatgcaaaa taagggtcta tttcgtttcc ttcttcatct
     2341 tttggagatt ctggaactac aggatctatc attgaaagta atatttttga caataaagta
     2401 tttggatttt tcttaattaa atcattcata aatgaaattc tttcatcatt taattttgta
     2461 aattcttcgg aaattttctt ttgctcatca ggattatctt ttgcagcttc gtatatatcc
     2521 ataagatcag ttcttctatt ttgaatatca accatttttc gctgatagtc cataaaagct
     2581 tgattttcct tatctccttt aactttcatt gtttgcaaaa agtttgtggt gtcggtttct
     2641 aaagaaaatt ttctattatc accaatcaaa agttcaaagt aagtcattcc gcgcgatggc
     2701 aaaacaacta tgtaaattcc tctttccagt tttttatctc cagcaaaaac tacatttcct
     2761 ttggaatcaa ctttggctgt gtcaacaaca tatttttttt ctccaaaatg atgacctaaa
     2821 tacgcaacag tatcacttac gccattgatt ttaattttaa tttcataatt ttgagctgat
     2881 aataaaaaac taataaaaaa ccccaatact aataatccaa tctttttcat atcataaata
     2941 gttaagttat tttccacaaa tttacaaata atatgacaaa atatgttcaa atactttgcc
     3001 aatcaatcaa tattttaaca aaaaattaag aacttatact taaaagttat cgaaccagct
     3061 tctacgttta gctaatttca agtcttttag catatcagct ttaacattta tttggaacat
     3121 ataagattta tgttgtccaa acggcactaa atgcagactg gcttcccaac agtgcaaatc
     3181 tctatatata tcaatagttg ttggcgtaac tttgttgttt tcaaagtcat aacctgtatt
     3241 aaaacttatt ttccatttag gagttaaatt aaaatctcca aaaacattta gagtttgtgt
     3301 cgttttagat tcaaaatatg gttttgcata tctgaggcta tagcttattc tcacgttcca
     3361 aggaatatta aagtcaacat aatcgtttgg atagccgtac aaaacattat atgggtcttt
     3421 atcttgtgat acaggggctt tagattctcc aacttttgaa tttaaagaaa tgctagcaga
     3481 aaaatttaat gttgttaatc ttaaaatttg cttattaata tctttagcaa gtttatttat
     3541 tcttacacca ccagagccat atgcgttttc tacaacagcg taagggtcta gattagcatt
     3601 aaagtttaaa tcaataagtt ttgctcttgt cctagccgaa actgcaattg gcgaccaatt
     3661 taggctgtca gtcataatat tatagcttga cgatagatta aagctttcta agagttttac
     3721 tttttgatca gtttttgtag tgtctttgct atttcttaat ttcatctcaa gattattgcc
     3781 taaattaaga tttaaacttc ctgctccacc tctcgctggt gcaccataag gcaagccttc
     3841 gaagcgtgag taataataac ttactgtgtc gtattgtcca gttgttccat tatattgtat
     3901 tctgtaataa tcgtcgtaat atttatattt aggttttccg aaatctggag cataactaaa
     3961 gctaatagct ggcgataata catgacgaag ggcttttagt tttcctcgtt taaagttaaa
     4021 agttccgtat aattgggttg accagtttaa actagccgaa taatcccaaa ctctagaaaa
     4081 accagaatta taatttgaat tgagaaaacc tatagtgtca ttttcgtttt caaaatccca
     4141 aaatttatac atgttcttaa aataccatcg ctcattataa ttaaatgcag gggttagagt
     4201 tgtgtatttt aaaaattttg cagaagtact aataggtatt ttgtgagaaa tgccattaat
     4261 catatcatca aaatttatat caaataatgt tgtatctgta gtgttaattt tatttgtaag
     4321 attcatagaa taggcaatgc caattttttc ataaatttta ctttctccaa cttttacttt
     4381 acgtttaaaa ggataaattc tattcatgtt taaagataag tcaggagctg tgagagttat
     4441 ggctttagtg ttattgtttt gattatgtct tatagcagct gttaaactaa atggagaatt
     4501 tggaaaaatc ttgctaatag aaatactcga ctgtttgttt gatgacatat agttgtttag
     4561 tgagtatgaa ttatatttgt caaaatttgc agaactcata ttaacatcgg cagcaaaagt
     4621 tgtgttaggc aaggcttttg ggtcttgtgt aaatttccaa ttaacagcaa ataaatctgt
     4681 aacttgtcta ttaacatctt tatagcctgt catatttcta ttatacttaa aattaagatg
     4741 tccgctaaat ttatatctta ccttataatt tgaaagcaaa gacacacccc aagaaccttt
     4801 tgtgtaaata tcgccgagaa aagttaagtc taaataatcg ttaacagccc aatagtaacc
     4861 gccatttctt aaaaacattc ctctattgtc ttcatttcca aattcgggaa ttaatattcc
     4921 agaagtgcta ccttttttgt tgggaaaaaa cccaaatggt attccaagag gagtgggaac
     4981 atcttctatt acaagatagg caggaccaga aacgagtttg tcatcaggaa taactttggc
     5041 tttacttatt gcaaaataat aatgcggatg tgctaaatcg caagttgtaa attttccatc
     5101 tatcatgtga atatctttat tttcttgcat tttagtttta gaaccatgca aaaatgcacc
     5161 ttcaaattct gtcattactt ctttaataat tccttttttt gttttgaaat tatatctaac
     5221 agtatctgca ataaattctt cttcgccttc tttaaatatc ggttttccat aaatattccc
     5281 agccgaatcg gttaaaccat gtgcgagaac ttcgttttta tcgaaatcca tttgaatata
     5341 ttcggcactc atgtttatat tctcataaat aaccacagct tttccatata aatatgcttt
     5401 ttcagctata aaatcaaata ataaagagtc ttcggcatcg taataaacgg gataatcaaa
     5461 actcatactt gattttttta tagaaacgct atcaactttt gtttgaggtt ttattgtatc
     5521 atcaaccatt tgataagcat taatctttaa ctcatatttt gtggtataaa gattgttgtg
     5581 tgcattaatt gatgacacaa aacttacaat tatggttaaa aataaaatta acaaaaattt
     5641 tttttgcaat atgaaattac tacttttgta caaaataaaa attttggtca aaactaatga
     5701 aaaaaacctt ttgtttaata aaatttttgc tttaatgata aaaaataagt gtttttttat
     5761 ttttagtctt gttttttttc tatttggaac aggaaaaata gcaaattctc aaagttttgc
     5821 tataaaaaca gttgttatag atgctggaca tggaggtaag gaccctggag ctgtaggcaa
     5881 aatttctaaa gaaaaagatt taacattaaa aatggcatta atgtttggtg aggaaataaa
     5941 aaaagctttc ccaagtatta aagtcattta tactagagat acagacaaat acctggcatt
     6001 acacgaaaga gcaaatattg caaataaaaa tcatgcagat ttatttatta gtattcatat
     6061 aaatgcttcg gacagtaaaa gtgcaaatgg aacagaaact tttgtgatgg gaattgataa
     6121 gtcgggagca aatttgaatg tggtaaaatt ggaaaactca gtgatattgc aagaagaaaa
     6181 ttatcaagaa aattatggtg gttttgatcc aaacgatcct gagtcgcata tcatatttag
     6241 tttatatcaa aatgtgaact taaatcagtc attagttatg gcacaggcaa ttcaagataa
     6301 atttacaaaa gatgtaaaaa gatttagcag aggcgttaag caagctccat ttttagtgct
     6361 ttggaaaaca tctatgccaa gcgtattagt cgaattaggt tttatttcta atttagaaga
     6421 agaaaaatat ttgaattccg atgctggaca aaaggcactt gtgaaagcta tgtttgatgc
     6481 gtttaaagtt tataaaaaaa gttatgaaga tgaatctgtt gagccagcaa attctaaaaa
     6541 taatgaaccc gaaattaaaa ctgaagtaaa taaagaggaa aataaaatag aaattaaaga
     6601 agaaactaaa gtagagcaag aagtaaaaca aacagaaaat caaaaaataa caaatgagga
     6661 aactgttgct aaaccagctg agtctgagat aattttcaaa attcaaatta tgacatcagc
     6721 aaaaaaaatt gaaataaaac ccaaaaattt taaatcatat cagaatgttg atgtatattt
     6781 gcaagatgga atgtacaaat atacaattgg aagagatagt gattatgata aaattgtaga
     6841 actacataaa aaagtaaaat tagattattc agacagcttt attgtggcat ttaagaatgg
     6901 agagcgtatc ccgcttaatg aagcaagaag ccaacaaaaa aataaatgaa caaaaaaaag
     6961 aaatacatat tttactcatt tgtcgcaata gcaatattgg ctgttacata ttggggtttt
     7021 aattttttaa aaggtgtatc tttatttaat aaagtgaatt cctattatat ttattatgac
     7081 agaattgaag gactgaatga atctagttat gttactgtga atggctataa agtagggcaa
     7141 gttggcgaaa taaaattatt gcctgcagaa aattataagc tgttggtaag aattgatata
     7201 actaatgaat ttgcaattcc agattctact atagcacgta tttatagcat ggatttaatg
     7261 ggaactaaag gaattgaact tgaatttgga aacaaaacaa catatattgc acctgaaggt
     7321 tttttgattg gacaagtaga gcaaagctta aaagaccaag taaatatgca gatgttgcct
     7381 gtaaaacaac aagccgaaca gttgatggca gagctaacac atgcaattac tgtgataact
     7441 tatatcttta acgaagatac tagagctaat cttgaaaaaa gttttgcaag cattaaaaat
     7501 actttagttt atatcgaaag ttctgcaatt agtgtagatg gtatgttaaa agaagaatca
     7561 actaagatag caagaacttt gtctaatctc gagtttataa cagctatgtt aaaagataat
     7621 tcagaaaata tggaaaatgt ttttaaaaat ttatcttctt tttctgacac tttagttgca
     7681 attgatatta gcaaaacaat ggctcaagca aacgatgcta ttcaaaactt taatcaaata
     7741 ttagatagag caaatagggg cgaaggaagc ttaggaaaat tattgaatga tgacaatttt
     7801 gcacaacaaa tagaagatgc tgctactaat ttaaaaagtt tacttttcga tatccagtat
     7861 aatccaaaaa aatatgtcaa ctttagctta atgaatattg ggcgaacagt taatgttgct
     7921 gacccttcgg agctttcgaa aagagataag cgagttttag aaagacagct tgaaaagagc
     7981 gaaaaagaag ttcaaaaaaa ttatgaaaaa gaacaaaaaa gacaagaaaa agaaaataaa
     8041 aaagatgatt tatcaaatta tcagggaaag gtaattttta tgattcaaat tcgttctgct
     8101 gtcgataaaa tagatttaaa ctctgatgaa ttaaaaggtt atacagatgt aattgaaata
     8161 aaatcagggg cttattacaa atatttaatt ttccctcacg aaagcacctc acaaacagat
     8221 tattacctaa atttagcaaa agaagatttt tcagatgctt ttcctgttgc acttgttgga
     8281 gatcaagttg taagttatgc aaaaggtatt gctatagtgg ctggggaata aattcagtta
     8341 aaagttaaaa gcgatgcact gaacgtagtt gaagtgttaa aagaggctgt cgcgtctatt
     8401 gcttcgcaga gttcgcaagc tcactctgct cgcaatcaca acacttatga cactcgctcc
     8461 aacacccatg atgcttgctc caacacccct gacgctcgct cggatttgca atccgagtgc
     8521 ttggtaatgt tacactcgga ttgcaaatcc gagcgagcgg ggtagtaggc aaacgtttgt
     8581 ccgtagcaaa taagttaagg tgtttttgtc tgaatttcaa aatttgtcta agtttttata
     8641 ttaaaaatga aaccttattg gaaaaaaatt acgcttaata taaaaaagta agtttttaag
     8701 aacatttaaa ataaataaat gttaaatttg aaaaattaaa attcagacgc tatggatatt
     8761 tatgaattaa taaaatcaag acgtagtcca aggggttatt atgataaagg tattaagcct
     8821 gaagatttag aaaaaatgtt tgatgctgct cgttgggctg cttctgcata taattttcag
     8881 ccttggcgtt ttatctatgc agataaagaa aaagatcccg aattatacaa tcaaatttta
     8941 gaattattgg ttgagtttaa tcaagcatgg gctaaagctg ctccagtttt aatagttact
     9001 ttagttaaca cacgaaaccc aaaaactgga gatacattaa ctcacgcaaa atatgatttg
     9061 ggcttagctg taggcaatat gactcttatg gcagaatcaa tgggaatttc tttacgaaat
     9121 atgagtggct ttgatgcgaa caaagcaaga gaggttttaa atataccaga ttatatggaa
     9181 gctgtaacaa ttattgctgt tggctatggc ggtttgcctg gaaatttgcc tcttgatatt
     9241 tataaaatgg aaattatgcc aagagaaaga atggagctaa acgaaattgt ttttaataaa
     9301 ccattcgaca aagaataaca tcagttaaaa gtttataaag ttcctaaagt ttataaagtg
     9361 taaacctatt ttaggacatg acaactttta acttttaact gagttacatg tatcggattt
     9421 taatttccac gactttatgc acaccactat ctaagtcaaa tttaaaacta tcaaatttgg
     9481 gtttagacat acctttgtga taataatttg aaaatccaaa accttcttga ggaataaaaa
     9541 atgaataatc cattttccca ttttcatttt catcatctaa taaacatact ccataagttc
     9601 ctgcaggaat gtcatcaaat cgaacagtaa ttttgccgtt cttcaagtct ttttttgata
     9661 tatacttatt tattgttggc ttctctattt tataatcttt ttcatttgta aagaaaccta
     9721 aatgtatcct tcctatagct ttttgtattc ctgtaatatt aaaagtaata ctttgagaat
     9781 gagctataat cccgaaactg agtaggaaaa ctaaaataaa actaattttt ttcataacag
     9841 taaattttta taacaaatat acaaaaaata tcttatctaa caataaaaca tacaaaattt
     9901 aagccaattt tttgatatat ataaataaaa aaaccgtttg cacaacaaac ggttttttta
     9961 ttttccagta agattttttt aaattatttt aattttttac aaatagcttc aaaagcttta
    10021 ggatgattca ttgctaaatc ggctaaaact tttctgttaa gttctatttt attagcatga
    10081 atttttccca taaattgaga ataactcata ccattgaggc gagcacctgc attaatacgt
    10141 tgtatccata atgctctaaa atttctcttt ttagtttttc tatctctgta tgcataagta
    10201 agtccttttt cataagcatt ttttgctact gtccagacgt tacctcttgc accaaaataa
    10261 ccttttgttt gctttagaac ttttcttctt ctgtttctag aagcaacgga atttacactt
    10321 cttgccataa ttttacattt tgcgaatgta tagcgttctt tttacagaat ctttcggcta
    10381 tagcactctg attaatactc tttttaatta tttcattccc aaacacaaca agatgttttt
    10441 cttatcagga tctgcaactg ttgttgtatg agttaatgct cttttacgtt ttatcgtttt
    10501 cttagtcaaa atatgacttt tataagcatg ttttctttta atttttcctg taccggttag
    10561 tgaaaatctt ttctttgctc cggatttagt tttcattttt ggcatcgctt caaatttttt
    10621 aattaagtta ttattttttc ttactggttt tcggtgatag tagaatctgc attcttttgc
    10681 cttctaattt aggcaattgg tcaacttttg catattcttc taaatcttgg gctaatttta
    10741 ataataaaat ttcgccttgt tctttgtata atatcgaccg acctttaaaa aacacaaatg
    10801 ctctaacttt atttccttct tctaggaatt tttgagcgtg tttaagttta aagttgtagt
    10861 cgtgttcatc ggtatttgga ccgaaacgaa tatcttttat agtaaccttt accgctttac
    10921 tttgtatctc tttttgtctt tttctttgct gatacaaaaa cttttgatag tcgataactt
    10981 tacatactgg tggttctgca ttaggcgaaa tttccaccaa atcaagtcct aaattatctg
    11041 ctacttctaa agcttctctt aaacttacta taccttgttt ttcgatgttg tccccaacta
    11101 aacgaacaaa tggcgcgcgt atatagttgt tgattctgtg ctcgccttgt tttttgttct
    11161 gcgaaaaatt acttctcttt tctataacta aaacctcctc ttttaattag taaaatattc
    11221 ttttatctct ttatttatta attcaacaaa ttcttcaatt cccataactc ctaaatcgcc
    11281 ttgtccttgt tttcttactg atatagtgtt gttttcgact tctttttcgc caacaatcaa
    11341 tagataagga atgcggttta gttcgttatc tcgtattttt ctacctatct tttcgtttct
    11401 gtcatcaatt ccgccgcgaa tatcgtaatt ttttagcaaa tctaaaactt tttgtgaata
    11461 attattaaat ttttcactta ttggcagtat tacaacttgt tgtggagtta accataatgg
    11521 gaattttcct gctgtatgct caattaaaac agcaacaaat ctttccatag aaccaaaaat
    11581 cgctctatgt atcattattg gtctatgcat ttcattatca gagcctttgt attctaattc
    11641 aaatctttct ggtaaattat aatcaacttg aattgttcca agttgccaag ttctgcctaa
    11701 agcatctttt accataaagt ctaatttagg tccataaaaa gctgcttcgc caagctctac
    11761 agttggcttt agtcccattt cttgggctgc ctcttgaatt gcttgttcgg ctttttccca
    11821 attttcatca ctaccaatat atttagtttt gtcatcaggg tcgcgtaaag aaatttgagc
    11881 tttatattcg ttgaaatcca aagatttaaa cacatacatt acaagctcaa taattttttt
    11941 aaactcatct tttacttggt cgggacgaca aaatacgtga gcatcgtctt gagtgaaaga
    12001 acgaactctt gttaatccat gcaattcgcc atgttgttcg taacgataaa cagttccaaa
    12061 ctctgccatt ctgtatggta aatcacgata tgaatattga cgagctttaa aaatttcgac
    12121 atgatgagga cagttcatag gttttagcat aaatgcttcg ccttcagctg gtgtttgaat
    12181 aggctgaaat gaatcttttc catatttgtc ataatgtcct gaagttttgt aaaggtctat
    12241 atgtccaata tgaggtgttt taaccatttc gtagttacgc tttttgagtt cttcagttag
    12301 ccacgactct aagcggtctc taagaattgt gcctttaggc aaccataatg ccattcccat
    12361 accaactttt tgtgaaaaat ggaaaagttc taattctcta ccgatttttc tatggtcgcg
    12421 ttttttagcc tcttcgagca ttgttaaata ttcttccaac attttttgtt ttggaaaact
    12481 aatagcataa actctagtaa gctgcttgcg ggtttcatcg cctcgccaat atgcacctgc
    12541 taaagaaagt acttgaattg cttttatata acctgtgctt ggtaagtgag gaccgcgaca
    12601 taaatctgta aagtttcctt gttcgtaaaa actgattgtt ccatcttcaa gttcggaaat
    12661 aagttcagtt ttgtaattgt cgcctttttt ctcgaaagtt gatagagctt ctgcttttga
    12721 aacttctctg cgtacaacag gattgttttg ttggcaaagc tctcgcattt ttttgtctat
    12781 tgctggaaaa tcattttcag aaagtgcttg tccgtctggt aattctatat cgtaataaaa
    12841 accattttct atagctggac caattccgaa ttttgttcca gggaataaag attcaatagc
    12901 ttcagctaaa acgtgtgctg aagtatgcca aaatgcatgc ttcccttctt cgtcttcaaa
    12961 tttgtgtaaa acaatttttg catcttcata aataggacga gttaaatcgc ttaattcctt
    13021 atttatgcta atagccaaaa cttccttagc taatcggggg cttatgttct ccgcgatttg
    13081 caagccattt acaccttttt caaactctct ggtcgaaccg tctggtaatg ttatctttat
    13141 catcaaaatt ttttttcgca aaaataatca aatttaccat ataataagca agtaaatgtg
    13201 taaaaatttt attttcaaaa acttcgtgaa ataaatgtca atttgtaaat tagtttttac
    13261 ctttgagaaa atttaaaaat tagaacaaaa tgaaaagaaa tctactaatt tttgtgctta
    13321 tttttgtttt tgtaaatagt gcacattttg cttttggaca aaaagacgaa aaacagtttg
    13381 gagtaaaatt tagtggttat gtaaaaaatg atttttatta cgatacaaga gaagtagtga
    13441 atgggcgaga aggacatttg ttactttggc cccaaagtcc aagttatgat acagaaggca
    13501 gagatattaa tgctcatgga aaatttcatt ttttagcaat acaaacaaga cttacaggga
    13561 acataactgg accagacgct ttaggagcaa aaacttcagc aatgtttgaa ggagaatttt
    13621 ttggaagtag cgattctgat attaacggtt ttagattacg ccatgccttg ataaaattaa
    13681 attgggaaaa gtcagaaatc ttaacaggac aaacttggca tccaatgttt gttacagaaa
    13741 attcgcctgg aacagtttct tttaacacag gtttaggttt tcagccattt gctcgaaatc
    13801 cacaaattaa atatacgttt agattaggta atattagact cgcagcaact gcttttactg
    13861 agagagattt tcaatcaaga aatttaacag gaacgccctc gggtgactat attagaaatg
    13921 caaaaatacc tgctttgaac tttagtttta attatggtaa aaataataat gaggctaaaa
    13981 cagcttttaa tacaggcttt gctgttaatt ataaaaatat tagaccatct ttaactactc
    14041 ctttgggata tattactaaa gaaaatgtaa gtgcattgtc ggctcaagtt tatattatga
    14101 agaaatttaa acctttaagt gtcaaatttg ccgcattata cacagaaaat gcaaatgaaa
    14161 tgtttattcc aagtggtttc gctatatatg agataataaa cttaacaaaa atggaatatg
    14221 cgtatttgcc aactcgaaat gctattagct ggattgatat tgctacgaca ggagaaaaat
    14281 ggcaatttgg aatatttgca ggatataatc ataatttagg aacagttaaa aatattatgg
    14341 tgtcgcctgt tggtttaggt gtaggtattg agcatttata cagagtgtcg cctagagtat
    14401 attattttgc tggaaaatta caaattggtg cagaaatcga atacacagta gctgcttatg
    14461 gagatggaac ttataccaca aaagcaattc ctcaaaatgc tgaagaagtt gctaatctga
    14521 ggctattgct aggagttttt tatcatttct aaaataaaaa cataaactta tttcagtttt
    14581 agcttgtcaa ttactaataa tatcttaaaa actaattttt attaaataat attttttatt
    14641 tttgtaatga aataaaacaa atatagagag aacgggactg gtgttccaat cggtcttcaa
    14701 aaccgatagc aggcggataa aaccgtctgt ggcaggttcg attcctgctc tctcagctaa
    14761 taaattgctc tttttaaaaa gcatttatgt ctcgttcaaa aattattaaa attttttaag
    14821 atagactaaa gttttgttaa agtcttgaaa atgtgaaaca aaaagcgttg aaattgcgtt
    14881 atttttatta aattatgggt gaaaaaaagt agaattttaa aaaattttga atatgaaata
    14941 taaacatgtt ttaattagtt tttttagtgg aataattggt gctattattg ccgttttgat
    15001 tattgattcg tacaaaacat cttctgatgg tgaaattaaa aaccaacata gtttagtagc
    15061 agactattat ttgaataaac agcatcaact tcctgcaaca acggttgcaa atccatttct
    15121 atttagtcca agttcgagtt ttgattttac ttctgcagca gaaatgagtg ttcccgcagt
    15181 tgttcacgta aagactcaat atagccaacc aaattatact ttgtatgatt ttatttttgg
    15241 tacttcgcca cgatattcaa ctcctgtttt aacttctggt tcgggagtta tagtttcgcc
    15301 tgatggttat attgttacaa acaatcacgt tgtggaagat gcggaaacaa ttgaagttgt
    15361 gttgaataat aaaaaatctt attcagcaaa acttgttgga cgtgatgcta ctacagacat
    15421 cgctttgttg aaagttaatg ctaataattt acctttttta aattatggcg atagtgataa
    15481 tataaaaatt ggcgaatggg ttttagctat aggtaatcca tttaatttaa cttcaactgc
    15541 aactgctggg attattagtg caaaagcacg aaatattagc ataatgtcag aaaatatggc
    15601 tatagaatct tttattcaaa ccgatgcggc agttaatcca ggtaatagtg gtggcgcttt
    15661 agtaaataca aaaggcgaac ttataggaat aaatacagca atagcttcgc gaacaggttc
    15721 ttttgtaggg tattcttttg ctattccagt aaatatcgtg agaaaagttg ttgccgattt
    15781 agttgaattt ggagaagtac agagagctta tattggaatt gatttgttgg atattgattc
    15841 agatttggct aatcaacttt ctattagtaa aatcgaaggt gtttatgtgg caaatgtttt
    15901 agcaaatgga gcaggcgaca aagcaggatt gaaagcaggc gacataatta ctgctataaa
    15961 tgatactaaa gtaaattctg tttctgaatt tttagaatat ttaagccgct ttagacctgg
    16021 agatagagta aatttgtctg ttaagggtgg acgtacaaca aaaaatataa aactaacttt
    16081 acaaaataaa tatggcgaaa cttctatagt aaaatcagaa gctattgatg atttaggagc
    16141 taaattcgag gttttatccg actatgacaa agctcgctat agagtgaaag caggaattag
    16201 agttactgat atttcacaag gtaagttttt ggcaaatggc attaaaaaag actttattat
    16261 cattagagtt aataacgaat ttgtgaatac tgttaaagat tttcaagata taattaaaaa
    16321 atctaatgga agccttttta tagaagggtt ttatcctaat ggaagctctg cgtattactc
    16381 gtttagacta tagagttaaa agttaaaagc gatgcactga acgtagttga agtgttaaaa
    16441 gttgaaccga gcttgcgagc tcaccgaagg taaagttgaa agcgatgcac tgaacgtagt
    16501 tgaagtgtta aaagtaggct gacgcgtcta ttgcttcgca ggttcgcaag ctcactctgc
    16561 tcgcatgaat tagttttaaa ccacaaaaga cacaaaaggg acaatcagtt aaaagttaaa
    16621 agcgatacac tgaacgtagt tgaagtgtcg aaccgagttt acgagctcac cgaaggtaaa
    16681 gtcgaaagtg gattttggat tttagattta tgatttgaga tttgtggaat taattcagtt
    16741 aaaagttatt atcttattct aaaaaaagtt gatatttcaa caaataaata cattttttaa
    16801 ttagatgttt ttcggcaaaa actattttgt aattattaaa aatgtatatt tgtagtttaa
    16861 taatcagtta atttaataat tatgaatagt agcaaggcat ttataatttc aggttttgtg
    16921 ttgttgctga tgttttgtat tgtgtatgtt gcttatggtt ataatagtga aaaatctgat
    16981 gttcttactc aagctaataa tcctaaatct ttcaatagtt ttacagatcc tcgtgatggc
    17041 aatgtttata aggttgtaaa aatcggagag caagtatgga tggcagagaa cttaaggtat
    17101 ttacctagct tagcaggttc tgatatgagt agaattgagc catgttatta tgtttatgga
    17161 tatgagggaa caaatgttga ggaagctaaa gcaacaaaga attataaaac ttatggggtg
    17221 ttgtataatt ggaacgcagc tttttatgct tgtcccgaag gttggcattt gccaagtgat
    17281 gcagagtggg cacaattagt ggattttata ggtggagaag agatcgctgg aggaaaactg
    17341 aaagaagccg gtacagcacg ttgggataaa cctaatacag gagctatcaa caaatttggc
    17401 tttacagctc ttccttgtgg aatttacttt aaggaattta ttggtattgg ttctttcggt
    17461 ttgtggtgga ctgctacttt agatgataat ccctactatg atatgttgac acataaactt
    17521 attaatgatt atgcattgat tcgaatgatt aattacgatg aaaataatgt agattgtgct
    17581 ttcactgata cgaatagtgg gctttctgtt cgttgtgtga aaaatgtaac agtggtaatt
    17641 ggatttaatt cagctaatta agttcataaa tttaaagtga gcattattca tttataacac
    17701 ataaacctaa actgtttaat aaaccttgat acaaaacagt ttcagtttct tctgatttaa
    17761 agatttcgtc agtttttact aattccaaat aatcaacatt tattgttgag ctaccgcttg
    17821 gataaatatc taaaatataa tcttctaaat agtatgcggg ccaacttttg ttgcaatata
    17881 attcgcctaa gtaattaaaa tctttgtcag caataattac agaatataag tccctattgt
    17941 ttatataaaa attttcgttg aaatagaact ctaaaaaata taattctctg tatttatcat
    18001 aatgtgcatt ttggtatgca cattctaaag tatgaaaatg ataagttgaa ggagtttcca
    18061 taggcataat tgtatcaatt aaacgagact tgaatgaatg tgttataagt ttatcattta
    18121 tataatccca ttcaaaaata cttgaagaat aattatagcg tattaaagga agattgtttt
    18181 ttgaaatgga aaaataatcg acatgccaac ttgttggata ataaattcct tcttctatgt
    18241 atggaaaact atataatttt gaaacataaa atttttgttc tttaatatta tatcgtaaag
    18301 ctaatggaaa gcgattttcc ataaattcct tggtgccaac atgacctttg tactcgctaa
    18361 aagttgcaaa aataacatta tcgccaataa ctggaattaa attactattt agcgttggtg
    18421 gaagtaaatt agaaagtttt aaatgagctg gaatatctgt tgtgtcgatg ttataaggaa
    18481 acttagtttt aatatttccg ttataatcta ttaattgaaa ctgactgggt gttgtaatgt
    18541 caatttcaac tggacttgag taattgtaat aaatgaagat actgtcttta ttttgataat
    18601 agaaagaact taaagttcta gtactatcag ttaatatttc atgctctttt ttaccttcgt
    18661 caatattgta aaaagcgagc ttttttccgc tacattaaga attattatgt ttttcccatc
    18721 aatatttatt acttgctctt tagcaaaaac ataatcaatg tcgccgtctt ctaactcaat
    18781 ttgttttgat tttacaattt ttaaatcata aacacttggt tgaggttttt ctttgcaaca
    18841 agcagaaaat aagattgcta ttagcattaa ctttaaaaca gtacttttca taattatttt
    18901 tttgagagtt taaaaatgtc ttatttctaa acggtttaac ttgagaaata ttttgtaaaa
    18961 atacttaaaa tattttttta aaatttatat ttcggttgaa atcattgtgt tgcccttgcg
    19021 ggcaaggctt ttgtaggact aattgccgta ggttgccacc tacggttaat catatgttgt
    19081 cctccggaca aatcagttaa aagtcaaacc tagctagctt aacaaaacaa aatttaatga
    19141 aaaagtaagg tgttttataa gtgtccaaat gcaaggcatc tgagattgag aacgaaggag
    19201 cgtacttagt acgtgactga gtttgaatat cgaaagtaac gaagcagttg gatacttata
    19261 aaataccgtt acgcgtcaat ttttgcataa gtagcatgag tttcaataaa ctctcttcta
    19321 ggagcaactt cgtcgcccat tagcatagca aaaatatggt ctgcttctcc agcattttct
    19381 atagttactt gtcttaaagt tctagtttct gggttcattg tagtatccca gagctgttcg
    19441 gcgttcattt ctccaagacc tttgtagcgt tgtatatgta aacctgtttc tttgccttgt
    19501 cctagagttt caataatatc taatctttca ttttcagacc aagcatattg ttcagttttt
    19561 ccttttttaa ttaagtagag tggtggtgta gcaatatata aatatcctcc ttctattagt
    19621 ggtttcatat atctaaagaa gaaagtcata attaaagttg caatgtgaga accgtccacg
    19681 tcggcatccg tcataattat aattttatga taacgcaatt tttctaagtt aacagcttta
    19741 ctgtcttctt cagttccaat tgttactcca agcgcagtga atatattttt aatctcgtcg
    19801 ctatcaaaaa ctttgtgagg cattgctttt tctacgttca aaatttttcc tcttagtggt
    19861 aatatagctt ggaaatttct atctcttcct tgttttgctg ttccacctgc cgagtcccct
    19921 tccacaaggt aaagctcgca attttcggga tttctatcag aacaatcggc taatttacct
    19981 ggaagtccag cacctcctaa tacattttta cgttgaacta attctctagc tttacgtgca
    20041 gcatgacgag cagtagcagc taaaataacc ttgtcaacta tagcttttgc atcttttggg
    20101 ttttcttcaa gatagttttt caataagcta ctaacagctt ggtcaacaga aatactaact
    20161 tcagagttac caagttttgt tttggtttgt ccttcaaatt gtggttcttg aacttttaca
    20221 gaaataacac aggttaaacc ttctctaaag tcatctccat ttatttcaaa ttttacttta
    20281 gataataatc cgctttcttc ggcgtatgat tttaaagttc gtgttaaacc acgtctgaaa
    20341 cctgtgagat gagtaccacc ttcaatagta ttgatgttgt ttacataact atgaatgttt
    20401 tctgtaaacg aagtattgta ttgcatggct atttctacag gtacatcatt tttttcggtt
    20461 gaaatatgaa taatattttc tgtaagtttt tcacgtcctt ggtctaagta ttcgacaaat
    20521 tcttttaagc catgttcaga ataaaattct ttggatttgg ctattccatt ttcatcaact
    20581 tgtcttttat cttctaaaat aagtcttata cctttgttta ggaaagctaa ttctcttagt
    20641 cgagaagata aaatatcaac ttggtattct gtagttatga aaattgagtt gtcgggccaa
    20701 aattctacgc aagtacctgt ttcttcagtt tctccaatta ctttcacatc gtgtaatggt
    20761 tttcctatag aatattcttg gaaataaatt ttgccatctc ttttaactgt tgcagttagc
    20821 tttgatgaaa gggcattgac gcaagaaacc ccaacaccgt gcaatccacc cgaaactttg
    20881 taagaatctt tgtcgaattt tccaccggca tgtagaactg tcataacaac ttcaagagca
    20941 gatttgcctt ctttttcgtg taaatcaaca ggtattccac gtccattatc ttcaactttt
    21001 attgagttat cttcgtttat agtaacttta attttatcgc aataacctgc taaggcttcg
    21061 tctatagagt tgtcaacaac ctcataaact aaatgatgca aacctttagt gctaatatcg
    21121 ccaatataca tggcgggtct tttacgaact gcttctagtc cttctaaaac ttgaatactg
    21181 tcagcagagt aatttccatt tccatttttt ccatttgtgt gagacgaagt gtcattgcct
    21241 ccgttttttt ctaaaatctc tttattttcg tccatatcta aatattttta tgtctttttt
    21301 ttaaagcata caaaggtaag aaattttatg gatatttcta aattttttgg cttataaatt
    21361 tcttatagtt attaacaatt tagagctttg taagcaggtg tgtattagtt agttgcgtaa
    21421 aattgttaat agttttttca ctattatttg caagttagaa aattgataga aattatttaa
    21481 ttttcgttaa attttgttta gaataaatat tttaatccta gcataaaatt aaagccatga
    21541 gacatataat tattccaaac atattgtttt tgtcctgtta tgttgtttaa gtttaagaaa
    21601 gcttggaaat ttgaagcaaa actatattca ccatataaac taacgtcaac aataggtttt
    21661 agttttttct caacaatatt aaggtctgca tcgtattctt ttgcatatct agcaccaata
    21721 aaatcagcat taagtccaaa atttaattta ttatcgaatt tataatttgt gtttaatcta
    21781 acatcaactg ttggcatatg ccatggtttt tctctatttt taatgtaaga ataatgataa
    21841 taatgtcctt ttagattaat gctaagtttg tcgaataaat cgtttaaagc aatttcgcca
    21901 taacctgtaa atctttcaac gtttgtataa attaaaacaa atttattttc aagcatagtt
    21961 gtagtatcgt ttacaaaaaa tcccatatca tcaattttag aataattagc attcaaatgg
    22021 aaaaatactt ttctgctaat atttcctttt aaaccaacat caataacttg tgcataattt
    22081 gttattttat tagtatggaa attattgata aaaggattta tgtctgataa aaattccaca
    22141 gtattatttt gaacatatcc tgtaaaactt gcataaggaa taattgtgtt gctaatatta
    22201 tgttgaaact gaacatttgg ataaatatga taagctactg gctgtttgct taattcacca
    22261 gtagtgttta cacctaaaca aatttgccaa ttatttgaat aatgtttgaa atatgggttt
    22321 aaatcaagaa atgtttcctt aaaattgaaa acagcactat cttgtatttg gtcattgttg
    22381 aatacaaaac ctaaatccat accaaatcta tttttcttaa ctaatttatt taagtcggct
    22441 tcaaaatcta ttttatgttg tgtgtttttt gactgagtaa agaaatattg gtaagccaaa
    22501 tttgatttaa agttcattct tctctcagaa ctgttgtttg attctaaatt tatatttgct
    22561 ctgagcttat tatatctttg catttccatt tcttctctgt cgcgtggctt aacaaaatca
    22621 gttggtacaa taatatttgg gtcgtagccg taaaaatatg cttggtttga tttaaaacta
    22681 atatcagctc ctaaggttga tctatagaaa aatctttttg caaaagcatt aactaaatta
    22741 ttattaaatc ctgaaaatat tttttggtcg gcagaatttt ttacttttcc aaatgctgaa
    22801 tgatgtttta caaaagctcc tgcagaataa tttctttgtc ttgttgtatt gtaataaact
    22861 tctgcaagtg gcgacaaata atttcccatt cctaatttaa taagtccatt ataaagtggt
    22921 tttagaggtt cgccactaaa gcttgcagct gtaattggtg ttggtttaaa gctagtttta
    22981 aataaaacag gctcaatctt gtattcgaaa tgaggattaa cttcaacagt atcaactatt
    23041 gtaggaagaa attgcattct gtttgcatct tttaagacag gagcaaattc tgtgtaaata
    23101 ataattcctg tttcttccaa atttcctggt ttttcatctt gttgtgcctt tagatttaaa
    23161 cctaaaaata aaatggctat aattaaaata cttgttgttt tcatgttcaa aattttcatg
    23221 ttaattattc tattttagca ggttctggta aacttatttc gctttcttta ttctcattat
    23281 cactattatt atctgagctt tgtgtattat cgttttcttc ttcgccagat aagtttattt
    23341 ttaaatttaa caaatcttcg ctagaacgtt ttccctgttc ttcgtcaata atatttgcta
    23401 atttttggct agtttcgtct tttatgccat cagtttcgat aggatagttg ttaagcaaag
    23461 attgtaaagt atgttttgca gagaaaaaat cttctctatc ataataaata tcagctaaac
    23521 atattaaagt ttttgctacc caataagcat gtggcgaatt tgattgtgca aactcataag
    23581 ccaactcttc ggcaatgctg tctctttgca ttttgtaatg aatttttatt actctgtact
    23641 ttgattcagc accttcggca gattttgttt cggtagctat gttctcaaat aagttcaaag
    23701 ctcttattaa attgtcttta ttgtaatatg ctttagccat tttaaagtgt gattctcttt
    23761 tttcttcatc gttagacctt tcattatcaa tgatttgcat tgctgctgct atagtgttgt
    23821 cccaattgtt taagttgtat gcacatctga gttttcctat agaagccaat aatgttgttg
    23881 acttattttc ggataaagag tagagtttat cgtaaagact gtatgcctct tcccatgctt
    23941 ctttgtcaaa caaaatgctt gatgctttaa ttgtactttc ttcagtaaat gcacctcttg
    24001 gagcatttgc aacaactcta tagttttcta aagctaaatc tttttcttcg aaataaaaat
    24061 gtaattctgc tttataataa tttgcttcta tgtaaaattt acctttaggg aaagatttta
    24121 aataattcgt taatgagctt agtgcttcgt tatatttttt atcaacataa tttttcttag
    24181 caatttgcca cataatttca tcttgctcat cttcgctgat gtttgttccg cttgcatttg
    24241 cccaatcaac aaagccgtca gggttatctt gttctatata aacagcttta atcatgtcgg
    24301 cagcattttt agcttcttcg gtatttggat attcggcaat tacttgtttg taaagttcta
    24361 atgcttttgg ataattcctt tcgttgaaat atattgcacc taaactagat aaagaagctt
    24421 ttcgcattga cgaatttgga aacttttcaa tatgtctatt gtaataaaat tttgcagaat
    24481 cgggattgtt taaagtggta tgaaatgtac gtccaatttc aaaattagca ttgctcaaat
    24541 attcagattc aggatagttt tgtattagtc ttcttagtga ccatattttt tcgttaggtt
    24601 tttttaatcc accataagac aatgccattt gatataaagt gtagtctggc gaaatttttc
    24661 caatatcaat agctttttta taatattcag ctgctggagt atattctttt tcaatataat
    24721 agcagtcgcc aatacgattt aaaacatcat ttaaaatatc gctgttagca tatcctggtt
    24781 gaagttcaaa tttacgaaac caagaattag caactacata ttcttttttg ttaaaataag
    24841 cataacccaa attataatgt gcttttgaat attcatcaag actaacagag cctgtagtgt
    24901 ttacaaaatc tttaaacata gtaattgcat cttcaacatt tccaagtcta taattagctt
    24961 cggctttcca ataatgtgcc aaagcatcaa tggttttatc ataaattttg taatctaaag
    25021 acttgtcaaa cattgttatt gcttcattat attttaattg agtgaaatat tccaaacctc
    25081 taaaatatgt tagtctttga tatgcagcat caatttttgt attgcggttt tgcatttttt
    25141 caatagtcgc aatagatgct tgataatttt tagtgcttaa aaatgcgtca attaaatagt
    25201 cgtatgctaa atctgtacgt ggcgaatttg gatattcttt aatatataaa gtaaaagcat
    25261 taattgcttc gttaaaaggc gacatagaaa gctcaaaact taattgtgca aaattgaata
    25321 aagcatcttc cttaattttt ggattgtaat tatattttga agcaagttcg aaagaacgta
    25381 gagcttcaat ttttttatct aattttatat tacaatcgcc catgtggtaa gcagcgtttt
    25441 gacttaaagt gtctttcata tttgcaattt tgctaaaaga attagctgct ttatcgtatt
    25501 gtttggtgtt gtagtaagca aaacctaatt cataatagtc atagtcgttt gctgaacttg
    25561 ttgaatttac ataattattc aaatgtggaa ttgcttccga gtatcttcct tttctatagt
    25621 aagccgaacc taaaagttta tttatttcag ctgctctacc gccagattct ttttctaaaa
    25681 ttttgggagc atattcaatt aattcatcat atttttcttg taaataataa atgtgaataa
    25741 tgtaaaaagg agtgatagaa gcataagaag gacttttttc taattctaaa aatcccaata
    25801 aagctgtttg atattgttcg tttatatatt ttaggtgtga ataaaaatat aatgacattg
    25861 gtccataaat attatttgca tcttttgatt cgtaaaatgc tttagctgct ctgtcaaaat
    25921 cttttcttgc aaaatgactg taaccaagtt tgaaaaaata ttctgatttt tcatcggctt
    25981 gtagcaataa tctatctgtt ttttcgtacc atttaatggc atttttataa tctttatttc
    26041 taaatttaaa gtttgccatt tgaaaacttg cattattgct atttctgctt tcaggatgat
    26101 tttcaataaa atttgttatt tgaaattctg catcattatt aagtaaattc atagaagaaa
    26161 gagcataata aaaagctgct tcgtctttat aagatgcata ttcttgacca tcatgaagta
    26221 atatttctga aaataatttt tgagcaggaa catacattcc cttttcatat aaattaattg
    26281 cagaagtcca tttggtttcc atttcattgt aggcttgagt agtttgagct tgtatcgaaa
    26341 agcttattaa cacgactaaa gctgttgaaa taaatctgat tttcatgttt atactttttt
    26401 caaaatttat aaatattcaa aattacaaaa cttatcgtta gtagttttta caatctgaaa
    26461 tattaattaa cataatcttg tttattacta tatcgttagt gtttgattta cgataataaa
    26521 aattttaatt ttgttgttta tattttataa cctaattgta ataaatttta ttgttcaaaa
    26581 ttatggaaga cagtacatat ttgattaaga ccgaaaattt gaatgtttat caagatgaaa
    26641 atctgatttt agaaaatgta aatattgaaa ttgaccttgg cgagtttgtt tattttattg
    26701 gaaaagttgg tagtggtaaa tcgagcttgc taaaagtttt atatgcagag cttgatatta
    26761 aagaaggcga agcaaaagtt gcaggttata atttgaataa aattaaaaat aaacatattc
    26821 ctatgttgag acgaaaaatt ggaatggttt ttcaggattt tgaattaatg gcagatagga
    26881 atgtgtttaa aaatttagaa tttgttctga aagcaactaa ttggaaatca aaaattgata
    26941 ttgatactcg aataaacgaa gttcttgaaa gcgttggaat gttggataag aaaatttcaa
    27001 tgcctacaaa actttcgggt ggagaaaaac aatctattag catagcaaga gctttattaa
    27061 acaatccacc attgattttt gctgacgaac ctacaggaaa tctcgacccc gagagtgctg
    27121 attctcttat gaaaatttta cacgaacttc caaaacaagg aaaatctgtt attatggtta
    27181 cccacaatca tgcatttttg aaaaaatatc caggcagagt gtttttgttc gaagatggaa
    27241 aggttactga gcagatagaa caagggatag atctaatcag ttaaaagtta aaagcgatgc
    27301 actgaacgta gttgaagtgt taaaagttga aagggctgac gcatactcag ttgaaagtta
    27361 aaagcgatgc actgaacgta gttgaagtgt taaaagaggc tgtcgcgtct attgcttcgc
    27421 agagtacgca ggctcactct gcttgctcag atttttccac acatccccgc tcgctcggat
    27481 ttgtaatccg agtgtaacat taccaagcac tcggattgca aatccgagcg agcgtcatgg
    27541 gtgttgggag cgagcgtcag aagtgttgga gcgagcaata gtgatgctag gagcgagctg
    27601 gtgggttgtc cgtgaggaca cttcagttaa aagtttataa agttcaaagt ttataaagga
    27661 atgccaagca aaaaaccact tttaactttt aactgatttg ccgtctctta tctatacgaa
    27721 ttttaattta ttttccgcaa attttattaa attcaaggaa tttaatccgc aaatttgatt
    27781 gaatttgcgg aattttgttt atatttgtaa aaaaatgaaa ttatgattaa tcgaatttta
    27841 agcaaagaaa tagataaacg tttgtttgca ggaaaagcaa ttattattat cggagcaagg
    27901 caggttggca aaacttcgtt gcttcatagt aaatttgatg gaaatccaaa tgtgctttgg
    27961 ttagaaggtg ataatataga aactcaatta ttatttgagg attttacact ttcatttgca
    28021 aaaacactaa ttggagacaa gaaaatttta attattgatg aagctcaaaa cattttgaat
    28081 attggcatta aattaaaaat tattattgac caaatgaaag atattcaggt agttgctaca
    28141 ggaagttcgt cttttgattt ggcaaataaa attaatgaac cacttactgg cagaaaatgg
    28201 gaatataaat tatatcccat atcttttgct gagatggttg cacatcacgg tttttttgaa
    28261 gagtacaaaa tgttggagca acgtcttgtt tacggttatt accccgaagt ggtgatgcaa
    28321 aaaaatgaaa atatggtaga agttttgcag ggacttgcaa caagttattt gtacaaagat
    28381 gtgttgaaat gggaaaatat tcaaaagagc gatagaatga taaaacttct gcaagcctta
    28441 gcttttcaaa ttggcggaca agtgtcatat actgaattgg gtaaaatctg tggattagat
    28501 agcaaaacca ttgagaaata cattctattg ttagagcaag cttttattat ttttagattg
    28561 aactctttta gtcgcaatca acgaaatgaa ttgaaattta gtaagaaaat ttatttttat
    28621 gataatggaa ttcgtaatgc gttgatagca aactttaatc ctgtaaattt gagagatgat
    28681 cttggagctt tgtgggaaaa ttttatgatt tctgaaagac agaaaaaaaa tcattattct
    28741 cattcctatg ccaattcgtg gttttggcga acacaaactc aaaaagaaat tgattattta
    28801 gaagaaaaag atggtgttat aaatgcattt gagtttaaat ggaataaaaa caaaaaaaca
    28861 agaatgcctt tggactttat gaaatcatat ccaaatgcta tttattccac aattacacca
    28921 gaaaattttt atgagttttt aagtatggaa taatccactt tccgtaaatt ttattaaatt
    28981 caaggaattt aatccgtaaa tttgattgaa tttgtggaat tttaacgtaa ccccgctcgc
    29041 tcggatttcc ccacacaacc cagctcgctc ggatttgttt tttgctttaa tttttagtaa
    29101 aatttacagg aggtaaaata ataggtccta attctgcttt cattgaaaga tttgtaatat
    29161 gttctcttat gtatggaaag attatggcag caccattgat ttttccaaat ttctcataat
    29221 cttttaattg gctttctcca atacattcaa atatccctac catctttact aaaaatgaga
    29281 attgttcggt attattatgt gtttttttaa cacttacagt ttcagcaaca gatataatat
    29341 tgttgttaac gctaacttct gtatctatat tcaaatctaa atttgcatct tcaaattgta
    29401 cattatttat gcgtttaaac ttactctcaa gtaaaattag attatttatt ctaaatccag
    29461 actccatttt attattagtc atattatgct gcttttagta ttgttaaaat ctgtgaaatg
    29521 ggaatttcaa tgttttcgaa tccactttct attttgtttt caaaagaatt aatgttgtca
    29581 aaagagttga tagtaaatag gtttagattt gtagaaaatg tgtttttaat tttttgggaa
    29641 gtggcaaccc aagaaaaagg tgcatattct aatccttcta gcactaattc aggattttct
    29701 attcctacta atgcgtcatc agaaataaaa caaatatttt ctgtaggaaa ttgagttata
    29761 aatctgttga aaatgtcatt ttcccaacat atataatcgt tatctaagtg ataaacctcg
    29821 tttggtaata cttcaacaac atgcaccaaa gcattttggt catattcgta acgaacacgt
    29881 gttttagaaa attttttgat aaacactttt aattcgttgt atataaagtt tttagcttcc
    29941 ataatcaata tcgctttaaa ataggtaata ctctttcaga aagttctatt gaattttgac
    30001 tttttttaat gtcaaaattt gagtcagaat aatcagcatc agttctaagt cttttgagtt
    30061 gcagaagatt atttcttaaa ttacgtatat cattagctga tagtttgttt tgtaaattac
    30121 caatatattt aaccacttca tttaacaagt attcatgcga accataccgt gattggcttg
    30181 tgttgctacg aagttcgtct tctgactttt tcatagaata aagctaaata tgtttcatca
    30241 attgatagca actataatat gcagcatgag caacagcagg ataaaaataa ttatcctgta
    30301 ataaagatgc tgaggcttca agtaattctg atttattttg tagatgactt ttgtgcataa
    30361 tactttgtag tgtatcaatg ttgcaaatat acaacttttt tgataaaaat atacacttta
    30421 tttgattatt attgtttttg tttcattttt taaagattta atattccaat ttattttaat
    30481 tctctcgctt atattttccg cactaccctg ctcgctcgaa attaaaaaat ctttacacat
    30541 tttgttgttt attttatttt ttataagttt gtttaaaaaa taaaatatga attcttttgg
    30601 tagaattttt agaattagca tttatggcga atctcatgca ggggaactgg gagttttaat
    30661 tgatggtgtt cctgcaggaa ttttattaag tgaaaaagat tttgaacttg ccatagaaag
    30721 acgaaaacct aatcaagttg gtagcacttc ccgcaaagaa gatgatattc ctttgattaa
    30781 atctggtgtt tttaactcct acacaactgg tgcaccaatt ttaatatctt ttgaaaataa
    30841 aaatattaag tctgaagatt ataatttcaa cggcttttat cgtcccggac attcggattt
    30901 tgtagcaaat aaaaaatata aatcatttaa cgattttaga ggttctggac atttttcggg
    30961 cagaatgact ttagctcttg ttgcagctgg tgttgtggct aacaaaattt tgcctgatat
    31021 taaaatttct gcatctgtta tttctgtcgc tggaagtaag gattttcaaa caaaaattga
    31081 tgaagcagaa caaaatggcg attctttagg tgctgtggtt gaatgtagaa taaataattt
    31141 gcctgtgggt tatggtgagc cttttttcga ttctttagag tctgttttgg cacatttgat
    31201 tttttcaatt ccaggtgcta aagctattga gtttggaaat ggaatttttg caggttcggc
    31261 aaaaggctct gaatttaatg atgtgtttat aaatgaaaat ggcgaaacaa aaacaaataa
    31321 ctctggcgga ataaatgcag gaattagtaa tggaaatgaa atagttttta gagtttattt
    31381 tcgaccaagt gccagcatct caaaagctca agagactttt aattttgaaa caaaaaaaat
    31441 gggcgaacta aaaattcaag gacgacatga tgtttgttat gctttaagaa cgcctgtaat
    31501 tgtagagtca gttgcagcaa ttgtgttagc tgattttaag cttattttat cttcgcaaaa
    31561 attatgaaaa gttttttatt tgaagtagcc caaaatatta ttcagcaaaa agatgatttt
    31621 caaaatattt gcattgtcgt tccaaatcga agaacagagc tgtttttgaa aaaagaaatt
    31681 gtaaatagcc tgaacgaaac ttcttggctt ccgcaaatta ttacaataaa atcaattttt
    31741 gagtcaaatt ccaatttaat agttgaagat gatttgcttt tgataagtaa actttttaat
    31801 gtatataaaa actgcactgg ctcaaacgaa aattttgata atttttacta ctggggacaa
    31861 ataattttga gcgacttcga tgacgttgat aaatatttag ttgatgcaga aaaattattt
    31921 gcaagcataa atgatattca agaaattgat gcaaaattta gttctttcga tgaaaaagaa
    31981 ttagaaatta taaaaagatt ttggacaaat atagatattg acaagcccag ctctcataag
    32041 gcagaatttt tgtcattatg gtcgaaaatg tacgatattt attctgaatt tagaaaaata
    32101 ttattcgagc aaggcgttgc ctatcaagga atggcttaca gagatttagt cgaaaacatt
    32161 cacaatgtaa atttagaaaa agataaatat tttattgtcg gttttaatgc tctaaataaa
    32221 tgcgaaatag aagtttttaa atttttgaaa accaatagag aaactgcatt tttttgggac
    32281 tatgacgatt attacgtgaa aaataacgag catgaagctg gacgattttt aagaaataat
    32341 ttaaaattat ttacaaatga tttaaaatcg aacaatgata aaattaaaag ccaaaatctt
    32401 gatgttgagg taattagcct tcctacttct gtagctcaag ttaagttaat agaagaaata
    32461 ttaacagaat ggaaagccca acccgatttc gatatagaaa aagtagcaat cgccttagga
    32521 gatgaaaatt tgttaattcc acttatgact tctattcccg attttgttgg tgattacaat
    32581 attactatgg gttttccttt gaaaaataca aaagccttta atttttttag caatttgctt
    32641 tcgctacgtc aaaattcaag atttttaacc gatagtttaa agttttatca taaagatatt
    32701 gtaagcattt taacacaaac ttatatcagc gaattttttt ctaaagatgc tcaggaaatt
    32761 cttgaatata tcaataagga aaaatcaatt tatgttgatt taagtagttt tacgcaaaat
    32821 gaatttcttg caaaattgtt tgatgctaat gtaaaagatg ttcccgaaat tttaaattgg
    32881 ctgtcagaag taattgcaga tgttttggac ttagtttcta aaaaagaaga ttttattttt
    32941 gaaacagaat ttttgttaaa gctctcaaac tcaataaatt tagttagtga ttgttttatt
    33001 gatggaaaaa ttattattga aagaaaggaa attttattta aaattctttt aaatgctgta
    33061 aaatctgtga gcttagcatt tgagggggag ccacttaaag gaatgcaggt tttaggtttt
    33121 ttggaaactc gaaatttaga ttttgacaga gtaattatgc tttcgctaaa cgaaggtgtt
    33181 tttcccaaaa aatcagctgc tcaaacttta attccttata atttgcgaaa attttacgaa
    33241 cttccttcaa tagagtttca ggatagtatt tttgcttatt atttttatcg tttattgcaa
    33301 agagctaaag atgtgaaaat tctttatgct gcacaaggcg atgaaaaaat tagtggagaa
    33361 gtttcaagat ttgttactca aatcaagtat gaagctgatt ttgatataaa atttattaat
    33421 aaagcctaca agataagtct atctaacgaa aatgttttaa atgtaaacaa aactcctgaa
    33481 gttattgaga aaattcaaac tcatttgcaa aatggaataa gtccttctgc tataaattct
    33541 tatttaagtt gtgagtttaa atatttttta aggtatgtaa agggtttgaa ggaagttgat
    33601 gaaattgaag aaaaagaaga tgctgcactt ttcggttctt tgtttcatga aataatgaag
    33661 cttttgtatg aaccatataa aaatcaagtt ttaaataaag aattatttaa gcaaatacga
    33721 gcagataaaa atataaacaa gtgtaaaatt gaagcaataa aaaaggtttt taaagtaaaa
    33781 tcagaggttc aaattgcgga gacagaaaaa aaattaattg tagatgtggt tgaaaaatat
    33841 gttaaacgca tgttggattt tgatagccaa agtcctgagt ttagcattgt tgatttagaa
    33901 gcagaatatt ctcgagatat taaacttgca aatgatttta gcgtaaaaat taaagggaat
    33961 atcgatagaa ttgatagaca aggaaaaacg cttagagtta tagattataa aacaggaaaa
    34021 gttaaaaatt ctttttctga aatttcggaa ttgtttgagc aagatagaac atatgagcta
    34081 gatataatta tgcagttgtt tttatatgct tatattcttc gtgaaaaata tgattatgtt
    34141 tgtcctagtg tgtttagtct caatgagtta aataaagatt atgatggtag tataagcatg
    34201 aagcgagaaa agttaactga tttttcagat gatttattac aagcatttga agaaaattta
    34261 atttctttgt ttgagagcat gcttgagcca aatacaatat tttcgcaaac tacagattcc
    34321 gacaactgca aattttgtcc ttataaagga atttgtaaca aataaaattt ctttgtttgt
    34381 ataatttttg tattcgtaag tctttgatta gttgtgtttt attgtgtcgc ccttgcgggc
    34441 gagtgggtgt gggggtgtat atccggaggc tgtctcaaaa gtaaaaacag cctctttttt
    34501 ttcacctttt ttctttgcta
//
LOCUS       DAOPNX010000017        34385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_1658_length_34385_cov_6.602884, whole genome shotgun sequence.
ACCESSION   DAOPNX010000017 DAOPNX010000000
VERSION     DAOPNX010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 34385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34385
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1658_length_34385_cov_6.602884"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..360
                     /locus_tag="PKN53_03205"
     tRNA            286..360
                     /locus_tag="PKN53_03205"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:319..321,aa:Glu,seq:ttc)
     gene            complement(827..900)
                     /locus_tag="PKN53_03210"
     tRNA            complement(827..900)
                     /locus_tag="PKN53_03210"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(864..866),aa:Ala,seq:ggc)
     gene            complement(907..2049)
                     /locus_tag="PKN53_03215"
     CDS             complement(907..2049)
                     /locus_tag="PKN53_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD87992.1"
                     /translation="MKKLLFSSLCLLSTLFAFSQISIVRSDVFEANDTIPEIYYGFEN
                     DNGCVATQEVMPENLVFGSQSAFPLIMLDSAIYYQPSEIDPNGVFEAANCAYLTKEEY
                     VMYLKITEDKVELIGMQAQMPMSETEMIDVKFDEAVALYNFPCEMGNNNSSHGIAKEK
                     RPLSDFEEMLGNYYGMLAFTFDTVRFYVQVDVISNFDEFGTIKFVGDSIKNGEYEYLR
                     EDLLMMTAIDIQLRQKSDGEYVSAGTLPGVPMELPMIDSIFNHNYWTKNNKKPMLTIA
                     YNFDKSSIHSMTFRYEYTSFVNTNIVANLNVYPNPTSKYINFSIDNCEDYELYIFSTD
                     GRLINRSALESNNTLIDISNYHSGVYLYQILGKNNTLIAGGKFIKE"
     gene            complement(2062..2475)
                     /locus_tag="PKN53_03220"
     CDS             complement(2062..2475)
                     /locus_tag="PKN53_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD87993.1"
                     /translation="MKRFLNFAILFAIILGGFFLSFGCKKPNPPKAIVFVVDEDNKPV
                     QEARVIVKAANSDSAHTMVYLLNETKRVADTSYTDKEGKVYYDFKYESIYKVEVTKGT
                     DRTHPFVRRGIGILILENDKTAEAKIQINEQTVFN"
     gene            2551..3414
                     /locus_tag="PKN53_03225"
     CDS             2551..3414
                     /locus_tag="PKN53_03225"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOD87994.1"
                     /translation="MNIKNIFFLTFLILAFCSCSSVKKASKINEYAVLETSEGSIIIG
                     LYEATPKHKNNFIDNVNKQIYDSVLVYSVVPNGIIKMGLPQKIEEIDYLSQNYYDSEL
                     SCEINPYLINKKGAVGMWRLPNEKNVNKFSDNKLFYICEGMQTNDEVLNTLVAKRNAP
                     LIADYISVLLKEPEYIHFEDSLNYYKTNGMNKEWTALYKQLTEKIIPRIEKDGKKLFT
                     LNNYQKNTYNTIGGVPIYDYEYVVFGEVVKGMEVVESISKVKTGLFNKPKKDIFILNS
                     KIISKKEFKKL"
     gene            3457..6330
                     /locus_tag="PKN53_03230"
     CDS             3457..6330
                     /locus_tag="PKN53_03230"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOD87995.1"
                     /translation="MKKFFIFCLSLVFASFSYAQTKDLVVKSYKLKNGLSVYLNEDHS
                     VPNVLGAVIIKTGGKYDPADHTGTSHYLEHMMFKGTDKLGTIDFEKENKILAKISELY
                     EVLAKTSNEDERKEIQKEINKLSLEAGKYAIPNELDRLLDQIGSSMVNAFTSDEIVAY
                     FNMFPNNQMEKWIILYSHRFQNPVFRLFQSELETVFEEKNMYDDEFITPLFEKFYSNL
                     YKKHPYGTQTVIGKAEHIKNPSLSNMRKMFDTYYVANNMALVLTGNFDMDRTIPLIEK
                     YFGEWKEGKVPEFPEYKEEPFNGIERVNVRMSPIKIGALAFRTVPTNHKDEVILNVCS
                     ALLSNSSQTGYLDKLSTSGKLMASFNFDDIKNDHGAMICVFVPKIIGQSLKKAEKLIF
                     TEIQKLYTEEIDDKFFEAIKLNLVKEFQQDLEDPTDRAFMIGQAFVIDKPWEEVLKLP
                     EEIMAVTKEDIKRVASQYFSENYLALHSKMGFPKKQKLEKPGFDPIIPENTEAKSQFA
                     KEFEEIKDEKSIPEFINFEKDLTIKSHDNGFTYFHVENKINDIFNIKLKFKVGNLTDA
                     RYEQLAEYLSLVGSKDMPFDDFNKALQEYGCTFDVEASNNFFIISVDGFDKYFKESMN
                     LIMSFIQNPDDDKSKLKQLRQNAMFTRKYEASSPDELGDALYNFGVYGINSPYLRRLT
                     TKQVSSLTTEDLLSLLQECITYECNIHYSGTLKPDELINYFESTLKERKTKTNDKFPK
                     VLPRQDYKENTILFLNDKKSLQSKIYFYIDGNKNDMKERAIASAFNQYFGTGMSALVF
                     QEIREFRSMAYSAYAVYRSGETLKEKGYLKAFVGTQADKTIDAIYIMDSLIHKMPVKD
                     GRIDNIKSSLLQSINSSKPSWRELSESVESWLLQGYKEDPRIDRFKIFNSLDFKNILI
                     FHETNIQKRPMLITIVGNKEKIDFQELEKFGNIIELDKKQILN"
     gene            6364..7635
                     /locus_tag="PKN53_03235"
     CDS             6364..7635
                     /locus_tag="PKN53_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD87996.1"
                     /translation="MKKALPIVFILISIYSFSQTTIKLMNYNLLNFNNYTSYCDQTNN
                     SHVAKAGYLKTIVQNQEPDLLAVCELGTSPSVTYTINYILGNSLNVGGETKWAATNTS
                     SSSYLANGLFYDKNKFEYIGHEVVATGIRDINIYKLKYIENGENILMNIVVLHLKAGN
                     SSSDASQRGEMVDYLMTRISNLGNSNENFIVTGDFNVYTSNETAFQKLINPNDIQYTF
                     YDPINQIGAWGNNYTFREHHTQSTHLDDNDCHSYGGMNDRFDFILIAGSIKDGSANIK
                     YKTDSYKAVGQDGLRFNKSLISPSNTSLPQEVITALYEMSDHLPVVAEFYMGEQNSSI
                     SLTTDPQFYANVVNPIDDKLVFKLQTIYKNKIINLRLMNISGAIVYETQILTQTDQIY
                     SYDMTGLSKGVYILNFNGEGINQSVKLVKTE"
     gene            7642..8103
                     /locus_tag="PKN53_03240"
     CDS             7642..8103
                     /locus_tag="PKN53_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOD87997.1"
                     /translation="MPKDYSQLIKKLISLEPKKVDDYFHSRHKKEFEKINCLECANCC
                     RNLGPLLTDSDIARLSKHLKIKPSEFTEQYLRIDEDNDYVFKTMPCPFLQDDNYCSVY
                     EFRPKACKEYPHTDRKNILGILDICIKNTEVCPAVANIFNKLNNDKFLMKT"
     gene            8162..9244
                     /gene="porQ"
                     /locus_tag="PKN53_03245"
     CDS             8162..9244
                     /gene="porQ"
                     /locus_tag="PKN53_03245"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HOD87998.1"
                     /translation="MKKLATSIFLIFTISFLFAQSGGRNTYEFLNLPAGVRTAALGGV
                     NVSHYDDDISFVLSNPALLKPNMNEKVTLNYINYISDINFGYASYAREFKNIGMFAAG
                     LQYINYGNFLYADEFGDIHGDFSAAEYALNISYANQLNSKISYGGSFKAIYSDFYQYF
                     SSGIALDAGISYLDTAKLFSAGLVIKNAGFQLKPYTKGNQEPLPFDIQIGVTQKLKHA
                     PFRFSITAHNLTRWNLRYKSIFDNTYELNTSENLADTTFGQKLTKFMNGVGRVGDELI
                     RHFVIGVEIVPTNNFYLAVSYNYRRRSEMRIETSPKIVGLSFGAGIKLSKFNISYALA
                     SYHLAGMSHHISLGLNLSEFYKSQKL"
     gene            complement(9710..10888)
                     /locus_tag="PKN53_03250"
     CDS             complement(9710..10888)
                     /locus_tag="PKN53_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOD87999.1"
                     /translation="MARKFKENLYLLDVVDWNRRLFDALIPLPDGTSYNAYFIEASEK
                     NVLIDTAEPELAHIFFEKLEGIKKIDYVVSLHSEQDHSGLLPEVLKKYPNAKLICSPK
                     AKPTLSDHMPIAEDRFITVEDGEEISLGNKTLKFIHFPWVHWPETMLAYLKEDKVLFT
                     CDFLGSHLATTDIFVSNECEVYEAAKRYFAEIMLPFRNIINKNLPKLDEYDIEIVAPS
                     HGPMYDNPKFIIDAYKEWLSDTPKNVVIIPYVSMHGSVEKMVNHLVTALENRGIKAYK
                     IDLTVTDLGKLAITLVDAATIVIGTPTVLGEAHPIVSYATILANALKAKAKYVSILAS
                     YAWGGRAVEKIAGMIPNIKAEVIEPIYIKGAPTEADLKAVENLAEEISIRHKKDNLSL
                     "
     gene            complement(11184..13985)
                     /locus_tag="PKN53_03255"
     CDS             complement(11184..13985)
                     /locus_tag="PKN53_03255"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOD88000.1"
                     /translation="MKQLTFLIIALIFSCSFAFGQSMELTDKLPNDPSVITGTLKNGM
                     TYYIKANKQPENRAELTLAVKAGSVLEDEDQQGLAHFTEHMAFNGSTNFPKNELVNYL
                     ESLGMKFGPEVNAYTSFDETVYGIKVPTDDPEFIDKGLLVLYDWASELSLETEEIDAE
                     RGVIHEEWRMGQGAMDRVQRKYLQAIFHNSIYAKRLPIGTMEVVDNCDPEAIRRFYRD
                     WYRPDLMALILVGDFDAKEMEQKVIERFSKIKMIDNPRERVYAEIPDHKETLVCVATD
                     PEVPISMVQMFYKHPAQPNSTVADYKDGMVNMLLSSMLSNRLAELTLLENPPFVQGYA
                     GYSQFIGPKDVFISMGIVQNNDIKTTLETLVVENERMMQFGFTETELEREKAALLKRI
                     EKSYNERDKQKSENYVEEYKKHFLPPHSPFPGIEYEYELFKKYLPQITLDEVNAFAKT
                     VVTEENCVIVVIAPEKEGVTIPSEDEVLKIYQDASKIKVDAYVDKVSDKTLISDLAKP
                     TKVGKKTKNKDFDYQTWTLKNGVKVVFKTTNFKDDEILFEARSLGGYSLYDQKDDISS
                     KIADDVAEESGLGNYDKSELQKFLADKNVRFSTYIDESTEGLVGSSNVADFEVLLQMI
                     YATFTQPRVTETAFNSYMNKQKGLLENAKLDPQSAWSDSIRTVMSKNHPRRRVLTAEV
                     LEEANYRRVKSIYSQRFGDPSNFTFYFVGNFDAKLAKPLIEKYLGALPIVERSETYKD
                     LGVRPPKGVVEKTVYKGKDPKCMEIINFPGEFEYTYQNRLELDAVCKILSTRLLEEIR
                     EKESGVYTIGAYPSSSKIPYEQYSVTIFFSCDPEREEELKTKTFNIIKSLQTDGLTDT
                     DIQKVIEKEKREFETDVKENSYWKRLIIDVEDNNVSAAEYKDYLNMIESISISSMKKA
                     ANKYFDLNNYVKVQLLPEN"
     gene            complement(14106..15719)
                     /locus_tag="PKN53_03260"
     CDS             complement(14106..15719)
                     /locus_tag="PKN53_03260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOD88001.1"
                     /translation="MKKEFVSPLKFETNLSGNFGEPRHRHFHSGIDFRTYQNGKEIVS
                     VADGYVCRISVSPWGYGLGLYIAHPNGYTSVYGHLNEFRKDIQNFVRKIQYENKSYSV
                     DTLLNPDLFKVKKGELVAFSGNTGYSEGPHLHFEIRETKTEQPINVVNSVYDFKDNLP
                     PEISAIVFYPLSNNSFINGKAEKFFLKVVKRDGVYVCANDLPEVTGLIGLGVAYVDRM
                     NETSNRYGAKTVSLFMDNELIYKSNLEKISFETQASKNSMFDFEYLLKEKLHVHKLFV
                     EAGNELMIYDKTINNGMFSLNEKSKANFTVIITDYNNNSSELNFELIKDNKNYDLLER
                     DGYLVKYDSVFLYFDSEFRFETQIGSLFYDTNLKIEKGKKRKYSQSYIIGEDHLAVNK
                     DFKISFYINDEAYKYKDKLFIMREHNKKSNYLKPIIEQNFISAYSSYFGEFYLWIDTT
                     PPKIVPIRPKKTYTEENLNYLSFKITDDLSGISSYAIYINNRWVLTQYEPKTKTIFYQ
                     KDEYFEKSNSYKIKVVVKDNCNNEAILEL"
     gene            15887..16180
                     /locus_tag="PKN53_03265"
     CDS             15887..16180
                     /locus_tag="PKN53_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88002.1"
                     /translation="MDEDYKDIIIKLNDNIQSLMASYSTIKEDNIILSNEVNKLNSEL
                     NIYKEKLEEIENKYNNLRLARSIADGDSDDKDVKLKLNRIIREIDNCIALLNK"
     gene            16185..16496
                     /locus_tag="PKN53_03270"
     CDS             16185..16496
                     /locus_tag="PKN53_03270"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOD88003.1"
                     /translation="MNNEKLKININIGERTYPINILRADSKREELIRKAAQLINDELF
                     QYKSRGYKNRDEQDYMAMVLISISVRLMEMEDREDVSPVINELKKINFTIEDFLEKER
                     E"
     gene            16760..18301
                     /gene="rny"
                     /locus_tag="PKN53_03275"
     CDS             16760..18301
                     /gene="rny"
                     /locus_tag="PKN53_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOD88004.1"
                     /translation="MGTLTIILVAVGALIAGGAISYFMWDKALEKRKNKVFNDAIAEA
                     EVIRKEKELQAKEKYFQLKSEHDKRINERNQKMAAAENKIQQREAIIASRIEETQRMK
                     KELEIIRENLDKQLDRVNKRSEELEKLHREQVEKLEAISGLSAEDAKAELMESLLANA
                     RSEAMSLINEIIDEAKMTANKEAKKIVLETIQRVATETAIENAVTVFNIESDELKGRI
                     IGREGRNIRALEAATGVEIVVDDTPEAILLSAFDPVRREVARLSLHQLVTDGRIHPAR
                     IEEVVNKTKKQIEEEIVEIGKRTTIDLGIHGLHPELVRMVGKMKYRSSYGQNLLQHSR
                     EVANLSALMAAELGLNPKIAKRAGLLHDIGKVADEELELPHAIYGMKLAEKYKEKPEI
                     CNAIGAHHDEIEMTSLISPIVQVCDAISGARPGARREVIESYIKRLKDLEALALSYPG
                     VLKTYAIQAGRELRVIVGSEKISDSEAEQLSFEIAKRVQDEMTYPGQVKITVIRETRS
                     VNYAK"
     gene            18291..19286
                     /locus_tag="PKN53_03280"
     CDS             18291..19286
                     /locus_tag="PKN53_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOD88005.1"
                     /translation="MQNNKFTINNSKVLVTGGAGFIGSNIIEKLLEQNNCIVCLDNFA
                     TGKRENINKFVKNKNFKLIDDDIRDLNACQKACKDIDIVLHHAALGSVPRSINDPISS
                     NEANVNGFLNMLVAARDNNVKRFVYASSSSVYGDNIASPKIENKTGNALSPYAVTKQV
                     NEMYAKVFSDLYGLETIGLRYFNVFGKNQDPESVYAAVFPKFIKSLINLQPPTIHGDG
                     TQSRDFTYIANVIQANELAATTNNPKAINTIYNVAYGETNNLNDITNLLIQLLAKFKP
                     EIANIKPIHGPERKSDIKDSLASIEKAKSLLNYNPKFDVRQGLELTVKWYYENLK"
     gene            complement(19336..20358)
                     /locus_tag="PKN53_03285"
     CDS             complement(19336..20358)
                     /locus_tag="PKN53_03285"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOD88006.1"
                     /translation="MQDLNWTNLPFGYVKTHYNVRSVFKNGKWGEIEVTDSEHVTMHM
                     AATVLHYGQAAFEGMKAYCGKDGKIRLFRWKENARRLQDSARGIQMAEIPEELFFEML
                     KKVIALNKDFVPPYGTGASLYIRPVLFGTGARVGVQPADEYTFIIFVTPVGPYFKAGF
                     KPVDIAVVRDSDRAAPLGTGTYKVGGNYAASLVGTKRAKDAGCSSPMYLDAKEKKYID
                     EIGAANFFAIRNNTYITPESTSILPSITNKSLEQLAAHLGYKVERRPVAFDELSTFEE
                     VGACGTAAVISPIGDIKDLDEGKTYSFCKDGKAGPVSTKLYETLVGIQYGDIEDPFGW
                     VTVLDI"
     gene            complement(20494..21936)
                     /locus_tag="PKN53_03290"
     CDS             complement(20494..21936)
                     /locus_tag="PKN53_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOD88007.1"
                     /translation="MKYFKSVFVISIVLVLSLLSFSCKTLNNKENVGADKKKPKYIFL
                     FIGDGMGINHASLAKYFYKEKGDDISFTNFPYFGVCTTTSKNSVITDSGAAGSAIACG
                     EKANFGSISYYPWFSKDSLPVSFAKIAHENGKKVGIITTVSLNHATPAAFYAQSTSRN
                     LYYEIGSQLPESGFEFFGGGGLKHVKGSDNNQPDLYEKAKSNNYIVSSNIKDFEKLDK
                     KNTKYMFVNPKLGLESDVPYKIDRKSDDGYNLAQFVKTGIKVLENPNGFFMMVEGGKI
                     DWAAHSNDGATIVKEIYDMDLAIREAYKFYEKYPEETLIFVTADHETGGLSLGNAQMA
                     YESNFMLLDNQKISITNFSEKIKDYKENNTKYSVDDIWKLADEYFFTETPIYTASDST
                     RVLEAFNYYFYEKSPTTSLEPIIIYGAENPVASIFGNIMCTKAGVGFTTWAHTAAAVP
                     VFAVGVGADRFGGAIDNTEIKKILLELVEW"
     gene            22096..23562
                     /gene="cysS"
                     /locus_tag="PKN53_03295"
     CDS             22096..23562
                     /gene="cysS"
                     /locus_tag="PKN53_03295"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOD88008.1"
                     /translation="MQENLFLYNSLSRKKEKFTAIKPPFVGMYVCGPTVYGDAHLGHA
                     RPAITFDLLFRYFQHIGYKVRYVRNITDVGHLESDADQGEDKIAKKAKLEQVEPMEIA
                     QFYSDRYHRDMDKLNVLRPSIEPRASGHIIEQIELTQKIIDAGYAYVSNGSVYFDVEK
                     YNQKYNYGKLSGRILEDLYSNTRQLDGQDEKRNSFDFALWKNASPEHIMRWNSPWGQG
                     FPGWHAECSAMGHKYLGEKFDIHGGGMDLLFPHHECEIAQNTIAVGENSINYWVHNNM
                     ITINGQKMGKSLGNFITLDELFTGANKVLEKAYSPMTIRFFILQAHYRSTLDFSNEAL
                     QAAEKGLARLLKGVETLQGLKPSKENNFDAEKFRQDCYDAMNDDLNSPILISNLFEGL
                     KTINSVATGSEKIDEKNLNILKETYNTFLFNVLGLKLENEDAGNNEMLEDVVNMVLNL
                     RLTAKEKKDWATSDFIRDELTKIGIKVKDTKEGFEWEI"
     gene            23631..23966
                     /locus_tag="PKN53_03300"
     CDS             23631..23966
                     /locus_tag="PKN53_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOD88009.1"
                     /translation="MSTENKKPDKLFYSIGEVAEMFNVKTSLIRYWENEFDIIKPQKN
                     KKGNRLFTAKDIDNFHLIWHLVKERGMTLKGAKKKIKENLAETEHNFEAVKKLNEIKD
                     LLLAIKNEL"
     gene            23973..25067
                     /locus_tag="PKN53_03305"
     CDS             23973..25067
                     /locus_tag="PKN53_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOD88010.1"
                     /translation="MKIKDIINSIEEIAPLSLQEEYDNSGLQVGNLEKEISGILISLD
                     VTSEVVQEAIDHNCNFIIAHHPLIFNRLRKITGSDDIEKSIILAIKNDISIYCTHTNF
                     DKVNQGVSYKICEKIGLKNLKILSPEKNILEKIAVFVPTSHADIVRNSMFEAGAGQIG
                     NYDNCSYNLQGEGSFRASNNSNPFVGKIGETHFEKEVRIETIYPKYLRNKILQAIFKT
                     HPYEEVAYDIYKLENPHNNIGLGMIGELEQEIAEIDFLKLLKEKFNSTIIKHSEFLNK
                     KIKKVAVCGGSGAFLINTAKSSKADVFVSGDIKFHDYFLAEKQILIADIGHFESEQFT
                     KEIFYDIIMKKNPNFAVRFSEKNINPIKTF"
     gene            25069..25830
                     /locus_tag="PKN53_03310"
     CDS             25069..25830
                     /locus_tag="PKN53_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOD88011.1"
                     /translation="MAKAENKIDKSTQSVTEKLKNLYRLQLIDTKIDQIRILRGELPL
                     EVNDLEDEIAGLETRIANFKNDITKLNEDISFSENAKLDAEALIKKYTEQQNNVRNNR
                     EFDSLSKEIEYQNLEIELCDKKIREYKHEIEQKTAQIEEANEKLVDKQQHLEQKKQEL
                     DTIISETKVDEEKQNKEREEIAENIDARLLAAYSRIRNNVLNKLAVVSIERDACGGCF
                     NKITPQRQIDIKSSRKIIPCEFCGRILVDPDILEK"
     gene            26032..29100
                     /locus_tag="PKN53_03315"
     CDS             26032..29100
                     /locus_tag="PKN53_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD88012.1"
                     /translation="MRKISLLSALLLFVTITFAQTNFTATYNLAGSDNNVTSFTYNGD
                     TYEGITPQPLLKVGVSTSPSNNNYRATDWPLGATSGSDVFTGTIDLEKYIGFSIEPVT
                     GYTFTITSIKFGLGRSATGPRQFEWRGNADGFASTLSTYEALNTNLTNVDGVLTCLDE
                     NSNWLNNTLTPGAAYQNLSTTTEFRLYAYNSEKVGGTAGLQGNLIIEGTFVATGTTVL
                     TINSPANNAIVNEDQVEVSFSTTNFELGTDGKLEYKLNGGAPAYTTTSPFTITGLAEG
                     ENTIVFQLVNMDNTALDPAVTVTRTVTYEVPNTDPSLTITLPADNTTVNVNEVQVTFT
                     TTNFELGVAGKLEYKLNGGTANYVTTTDPFTISDLAEGENTIVFQLVNMDNTALDPVV
                     SVTRTVTYEIVVLEPSLTISSPQDGAIIYSNDININFTVQDFVLGIDGKLAYKLDEGA
                     IQYYTGTSPLTLTGVAYGTHQIYFELVTMADASLDPAVTTTVNFTNQEPLPGGMETFD
                     NANIGTSYADGSFIGNNNIQWTYTHSRDEGDYAINGKGLMLRRPSDSSIESQTLSGGI
                     ASFEVKFRKAFEGASERGLELYINGELKGTSDLIPGVSGANATIYTFSVDNINVPGDF
                     VIKIKIAGTTEYNRQITIDDISWTGYNSTEPYLAITSPSNGQIFYENTVSVNYTTTNF
                     TVGTDGKAKYIIDGAAPVYTTNNPITLTNLADGEHTFSLELVNMDNESYSPAVIRTIT
                     FTTNTEGPEFTDIYDIQYTLDESGDSPLKNQYVWVKGIVVANFNPSTYGEGYYIQQGG
                     GAWNGIYVADLVNSPTVGDSVIIAGKVVESYGLTSIKELTYFEAFATDGILAAPAEVS
                     TADAGTEAYESCLVKVIDAECTQGIISYGEWYVDDGSGELLVKENGVSSIQPVVGNHY
                     DIVGVIYYGYSKFSLQYRSDADIVITNINEDFANSLSLYPNPANSFVNISSEFEINNI
                     KVTNNLGQVVLEQNAKSNNVNLNVENLESGIYFININSNDKNTVLRFIKE"
     gene            29163..29612
                     /locus_tag="PKN53_03320"
     CDS             29163..29612
                     /locus_tag="PKN53_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88013.1"
                     /translation="MKSKLFIQLFAFSLLIFVSQSCKKYENGPAISFISAEKRIVGKY
                     ELETYKINGIEIKLSEIGLDNFEKEYFEDGKGKNYSRQFNNLIETEFEWELNKDKTQI
                     REREKGLNGEWGQWNSFYTITKLTKTEFWTLNENSAEQHEFHYLKIK"
     gene            29682..30104
                     /locus_tag="PKN53_03325"
     CDS             29682..30104
                     /locus_tag="PKN53_03325"
                     /inference="COORDINATES: protein motif:HMM:NF025032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOD88014.1"
                     /translation="MKKLTIILSIIVGATMLFSSCRKYEEGPAFSLATKTARLSGEWL
                     LKEVTVNNVAQDMSTEGQISMTLKKDQTGSMNITFFGATLSEPIEWKFSDDKLNLMIK
                     EVDDNEWNISEIIRLTSKELWLRDVDDNTTTVIKYEKK"
     gene            30223..32232
                     /locus_tag="PKN53_03330"
     CDS             30223..32232
                     /locus_tag="PKN53_03330"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009010755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOD88015.1"
                     /translation="MTKEEFQKLILQGIPEVLPPAANYEKNISHAPIRKQILSIEEKK
                     LALKNALRYFPKKFHAELAPEFKHELETYGRIYMYRFRPNYKIHSRDITWFPHKTIQA
                     AAIMLMISNNLDYAVAQHPHELITYGGNGAVFQNWAQYLLAMKYLAEMTDEQTLVMYS
                     GHPLGLFPSHKDAPRVVVTNGMMIPNYSKPDDWERFNALGVTQYGQMTAGSYMYIGPQ
                     GIVHGTTITVLNASRMISKHKDANDCKLFVTSGLGGMSGAQPKAGNIAGVVTICAEIN
                     PKAAYKRHEQGWVDEIVENADAAIDLALNFQAKKQAHSIAYLGNIVDLWEKLAERNIK
                     VDLGSDQTSLHNPWAGGYYPVGYSFEEANNMMANSPEKFKEAVQESLRRQVAAINKLT
                     DKGMYFFDYGNAFLLESSRAGADIMAKNGIDFKYPSYVQDIMGPMCFDYGFGPFRWVC
                     TSSDPKELELTDNIAMQVLEDIAKTAPKEISQQMKDNIKWIKEAKINKLVVGSQARIL
                     YADAEGRIKIAKAFNDAIKEGKLKHPVVLGRDHHDVSGTDSPFRETSNIYDGSQFTAD
                     MAIHNVIGDAFRGATWVSIHNGGGVGWGEVINGGFGMVLDGTADAERRLKMMLLWDVN
                     NGISRRNWARNEGAIFAIKRAMKEYPDMKVTLPNFADDELINKLY"
     gene            32251..34317
                     /locus_tag="PKN53_03335"
     CDS             32251..34317
                     /locus_tag="PKN53_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88016.1"
                     /translation="MKKIVFTLFIFTTIMAISCKNDNKPSQNEENIIEFKILDENIDE
                     AIQKIKEKNTDKEKFRIEKGVRQVAALWQQNDGDATEFINFCVDNFISDPEELKVAFD
                     KLERNFEILFGHYNKISVELMEPLHLDMGEIGFIDNVMGAYSPSAHLTEDLFENKLAF
                     YVALNFPAYSLSEKTKNADTWSRLDWAYARMGDLFISRIPAVLYQKEAEALTNADTYI
                     ADYNIYMGNLVNSEQKTFFPKDMKLITHWGLRDELKANYNMDNGFEKQKMIYNVMLRI
                     INQEIPQEVINKNEFTWDPIANKIYKDGKEASFTSEPFTRYEYMLYNFKALSAMDKYN
                     PVYPTFIERAYDEGMELSREEVEKLFVNFISSPEIKDLAKLIEKRLGRSLQPYDIWYN
                     GFKSGTNINEEELDQITRRKYPNTSAFEKDIARILIDLGWTRDKANYIANKISVDPAR
                     GAGHAWGAQMKGDQAHLRTRIGSKGMDYKGYNIAIHELGHNVEQTITLYDIDYYMLNG
                     VPNTAFTEAVAFLFQSKDLKLLGKEANSRNEESEALAALDNCWSAFEIMGVSLVDMYV
                     WQWLYENPDANPKELKEAVVKIATDVWNKYYYPVMGIKDSPILAIYSHSITSPLYLAN
                     YPVGHLIEFQVEQYIKDKNFAEEITRVLLQGRIIPQAWMKGAVGTEISGQPTLEAVKK
                     ALKIVS"
BASE COUNT        12585 a         5278 c         5381 g        11141 t
ORIGIN      
        1 aacttcaaag aatacctaac gatacaaaaa cattaagtgt tgaagaagct aagtcggttt
       61 tgaaacttat tgacgttcta gaagacgatg atgatgtaca aaatgtattc cacaatatgg
      121 aaatgacaga cgaaatcatt gaagcaatgg aataaaacaa tgcatacaat attgaaaatg
      181 agcaatttag ttaagttgct catttttttt gttttttttt ataaaaaatt tgtataatta
      241 aaaaaaagta tgtttatttg cagtcccaaa acagaagtgg gaaacggtcc attcgtctag
      301 gggttaggac gccaggtttt catcctggta acaggggttc gaatccccta tggactgcca
      361 aacaaaaaca aaaatcctta atgtgcaaaa tatcaacatg ttaaggattt tttatttggg
      421 atatatcttt ttcctgtttt cagcgttgca acacccaata caacaaacca tgctgattac
      481 caaagagtta gacgggtgtt gcaaaaaagt gcaaaaaata agagcgaaag ttatgatttt
      541 gtttatctaa aataactttt aaaaaaaatt gaaaaacatc ttttaacgac tactcttttt
      601 ctttaaattt ttcaacgaag aaactttgtt ttttgtcaat taaatatctt caaaaagtaa
      661 aaaagaaata caaataatgc taaggcaaaa atctttaatg aaaatacagc agctttgaaa
      721 tacaaataaa tagctagcaa tcccaacatt cctgctgtta tgtaaaataa aatgcttcgt
      781 ttttctttct ccataaaaaa acaaataatt atttcacatc tacttgtgga gtatatcgga
      841 ctcgaaccga tcacctttag actgccagtc taacgctcta gccagatgag ctaatacccc
      901 gcctctttat tcttttataa atttaccgcc ggcaattaga gtattatttt taccaagaat
      961 ttgatataaa taaactccag agtgataatt tgaaatatct attaaagtgt tattagattc
     1021 taatgcagac ctattaatta aacgaccatc tgttgagaaa atgtaaagtt cgtaatcttc
     1081 acaattatca attgagaagt taatatattt gcttgtagga tttggataga catttaagtt
     1141 agcaacaata ttagtattaa caaaacttgt atattcatat ctaaacgtca tactgtgaat
     1201 agatgattta tcaaaattgt atgctattgt taacattggt tttttattat tttttgtcca
     1261 ataattatga ttaaagattg agtctatcat tggcaattcc atagggacac caggtaaagt
     1321 tcctgccgag acatactcac cgtcgctttt ttgcctaagc tgaatatcta tggcagtcat
     1381 cattaataaa tcttctctta aatattcata ttcaccattt ttgatagaat cgccaacaaa
     1441 ttttattgta ccaaattcat caaaattaga aattacatca acttgtacat aaaatctaac
     1501 tgtatcaaac gtaaaagcaa gcattccgta atagtttcct aacatttctt caaaatctga
     1561 caagggtctt ttttctttag caattccatg agaggaatta ttgtttccca tctcgcaagg
     1621 gaaattatat aatgctacag cctcatcaaa ttttacatct atcatttcag tctcactcat
     1681 aggcatttgt gcctgcatac ctataagttc aactttatct tcagtaattt tcaagtacat
     1741 tacatattct tccttagtta aataagcaca attagcagct tcaaaaacac cgtttgggtc
     1801 gatttcagaa ggctgataat aaattgctga atctaacatt attaatggga aagcagactg
     1861 tgatccaaaa actaagtttt ctggcataac ctcttgggtt gcaacacaac cattatcatt
     1921 ttcaaatcca taataaattt caggaatagt gtcatttgct tcaaacacat cgcttcttac
     1981 tattgaaatt tgcgaaaaag caaataaagt actcagtaaa cataaagagc taaataataa
     2041 ctttttcatg gctgaataat tttagttaaa tactgtttgt tcgttaattt gaatttttgc
     2101 ttcggcagtt ttatcatttt ctaagattaa aatacctatt cctctacgaa caaatggatg
     2161 agtacggtct gtaccttttg ttacctcaac tttgtaaata gattcgtatt taaaatcata
     2221 atacactttt ccttctttat cggtataaga agtatctgcc actctttttg tttcgttaag
     2281 taaataaacc attgtgtgag cgctatcaga attagctgct ttaacgatta cccttgcttc
     2341 ttgtactggt ttattatcct cgtcaacaac aaaaactatt gcttttggtg ggttaggttt
     2401 tttacaacca aaactcaaaa aaaagcctcc taaaatgatt gcgaaaagta tcgcaaaatt
     2461 taaaaatctt ttcataactt ttattaacaa ttttttttaa ttttgtaaag tacaatatta
     2521 gtaatttttt taatataatt caaatttttt atgaatatta aaaatatttt ttttctaaca
     2581 tttcttatct tagctttttg ctcatgctca agcgtgaaaa aagcaagtaa aatcaatgaa
     2641 tatgcggtac ttgagacttc ggaaggcagt attataattg gtttatatga agcaactcct
     2701 aagcataaaa ataattttat tgacaacgta aataagcaaa tttacgactc agttttagtt
     2761 tattctgttg tcccaaatgg cattattaaa atgggtttgc cacaaaaaat tgaagaaatt
     2821 gattatctaa gtcaaaatta ttatgactca gaattaagtt gcgaaataaa tccataccta
     2881 attaataaaa aaggtgcagt aggaatgtgg agacttccaa atgagaaaaa tgttaataaa
     2941 ttttcagaca ataaattgtt ttacatatgc gaaggaatgc aaacaaacga tgaagtttta
     3001 aatactttag ttgctaaaag aaacgcacca ttaatagccg actatatctc tgttttatta
     3061 aaagaacctg aatacattca ttttgaagat agcttaaatt actacaaaac taatggcatg
     3121 aataaggagt ggactgcatt atacaagcag ctaactgaaa aaattattcc tcgaattgaa
     3181 aaagatggaa aaaaattatt tactttgaac aattaccaaa aaaacacata taatacaatt
     3241 ggaggagtac ctatatatga ttatgaatat gttgtttttg gcgaagttgt aaaaggaatg
     3301 gaagtagtag aaagtattag taaagttaaa acaggacttt tcaacaagcc taaaaaagat
     3361 atttttatct taaactcaaa aattatttct aaaaaagagt ttaaaaaatt ataaatttta
     3421 aaataataat attactaagt tttaaataaa ttatatatga aaaagttttt tatattttgc
     3481 ctttcacttg tatttgcgag tttttcgtat gctcaaacaa aagatttggt cgtaaaatct
     3541 tataaactta aaaatgggct atcggtttac ttaaacgaag atcattctgt tccaaatgta
     3601 ttaggagctg taataattaa gacaggtgga aaatacgacc cagcagacca cacaggtact
     3661 tcgcattatc ttgaacacat gatgtttaaa ggtactgata aacttggcac cattgatttt
     3721 gagaaagaaa ataaaatttt agcaaaaatt tcagaacttt acgaagtttt agcaaaaact
     3781 tcaaacgaag atgaacgcaa agaaattcaa aaagaaataa acaaactttc tttagaagct
     3841 ggtaaatatg ctattccaaa cgagctagat cgcttactcg accaaatagg ttctagcatg
     3901 gtaaatgcct ttacttctga cgaaattgtt gcatatttca atatgttccc aaataaccaa
     3961 atggaaaaat ggataatttt atacagtcat agatttcaaa atcctgtatt taggctattt
     4021 caatcagaat tagaaacagt tttcgaagaa aaaaacatgt atgacgatga gtttattact
     4081 ccattattcg aaaagtttta tagcaattta tacaaaaaac atccatacgg aacacaaaca
     4141 gttatcggaa aagccgagca tataaaaaac ccttcgctaa gtaatatgag aaaaatgttt
     4201 gacacatact atgtggctaa taacatggct ttggtgttaa ctggaaattt tgatatggac
     4261 agaacaatac ctttaataga aaaatatttt ggcgaatgga aagaaggcaa agttcctgaa
     4321 tttccagaat ataaagaaga accttttaat ggcattgaaa gagtaaatgt tagaatgagc
     4381 cctataaaaa ttggtgcctt agcctttaga acagtaccta caaatcataa agatgaagtt
     4441 attctaaatg tttgttctgc actgcttagc aattcatctc aaactggcta tttagacaaa
     4501 ctttcaacaa gtgggaaatt aatggcttca tttaactttg atgatattaa aaatgatcac
     4561 ggagcaatga tatgtgtttt tgttcctaaa attattggac aatctctaaa aaaagcagaa
     4621 aaattaattt ttaccgaaat tcaaaaactt tatacagaag aaattgacga taaatttttt
     4681 gaagcaataa aattaaattt agttaaagaa tttcaacaag atttagaaga cccaacagat
     4741 cgtgctttta tgattggaca agcttttgtt attgataagc catgggaaga agtgcttaaa
     4801 cttcctgaag aaataatggc tgttacaaaa gaagatatta aaagagttgc tagtcaatat
     4861 ttttcagaaa attatttagc attgcattca aaaatgggat tccctaaaaa acaaaaatta
     4921 gaaaaacctg gcttcgaccc tatcattcca gaaaatacag aagcaaaatc gcaatttgcc
     4981 aaagagtttg aagaaataaa agacgaaaaa tcaatacctg aatttattaa ttttgaaaaa
     5041 gaccttacaa taaaatcaca cgacaatgga ttcacatatt ttcatgttga gaataaaata
     5101 aacgatattt ttaatatcaa gctcaaattt aaagtcggca atcttacaga tgctcgctac
     5161 gaacaattag ccgaatattt atctttagtt ggtagcaaag atatgccttt tgatgatttt
     5221 aataaagctc ttcaagaata tggctgtact tttgacgttg aagctagcaa taactttttt
     5281 ataatttcag tagatggttt tgataaatat tttaaagaat caatgaattt aataatgtca
     5341 tttattcaaa atcctgatga tgataaatct aaactaaagc aattaagaca aaatgcaatg
     5401 tttacaagaa aatatgaagc aagctctcca gatgaattgg gagatgcact atataacttc
     5461 ggtgtgtatg gaatcaattc gccatattta agacgcttaa caactaaaca agtttccagc
     5521 ttaacaactg aagatttatt gagtttgttg caagaatgta ttacttatga atgcaacata
     5581 cattattcag gcactttaaa accagatgaa cttattaatt attttgaatc tactttgaaa
     5641 gaaagaaaaa caaaaacaaa cgataaattt cctaaagttt taccgcgtca agattacaaa
     5701 gagaatacta ttttattctt aaatgataaa aaatctttac aaagcaaaat atatttttac
     5761 atagatggca ataaaaatga tatgaaagaa agagctattg catctgcatt taaccaatat
     5821 tttggaacag gaatgtcggc tttagttttt caagaaatta gggagtttcg ctctatggct
     5881 tattcggctt acgctgttta tagaagtggg gaaactttaa aagaaaaggg atatttaaag
     5941 gcttttgttg gaactcaagc cgacaaaacc attgatgcta tatatattat ggacagctta
     6001 attcacaaaa tgcctgtaaa agatggacga atagataata taaaatcttc gcttttacaa
     6061 tcaataaatt ctagcaaacc aagctggaga gaacttagcg aatcagtaga atcatggcta
     6121 ttacaaggat ataaagaaga cccaagaatt gataggttta aaatttttaa ttctttagat
     6181 tttaaaaata ttctaatttt tcacgaaaca aatattcaaa aacgccctat gttgattaca
     6241 atagttggaa ataaagaaaa aattgatttt caagaattag aaaagtttgg aaacatcatt
     6301 gagttagata aaaaacaaat cttaaattaa ttttaattaa taaactataa cacacaaaat
     6361 aaaatgaaaa aagcactgcc tattgtattt atattaatta gtatttattc gttttcccaa
     6421 accacaataa aattaatgaa ttataattta ttgaatttta ataattacac ttcatattgt
     6481 gaccaaacca ataattctca tgtagcaaaa gcaggatatt taaaaacaat tgtacaaaac
     6541 caagaacctg acttattagc tgtatgtgaa cttggaacaa gccctagcgt tacatatact
     6601 attaattata tattaggaaa tagtttaaat gtaggtggag aaacaaaatg ggcagcaaca
     6661 aacactagta gttcgagtta tttagcaaat ggattatttt acgataaaaa caaatttgaa
     6721 tacatcggac atgaggtagt tgcaacaggc attagagaca tcaatattta taagttaaaa
     6781 tatattgaaa atggcgaaaa tattttaatg aacattgttg ttttgcattt aaaagctgga
     6841 aactcaagct ctgatgcaag tcaacgtggc gaaatggtcg attatttaat gactagaatt
     6901 agcaatcttg gcaatagcaa cgaaaacttt attgttacag gcgactttaa tgtttacacc
     6961 agcaacgaaa cagcatttca aaaattaata aaccctaatg atattcaata tacattttat
     7021 gatcctataa atcaaattgg agcatggggg aataattaca cttttagaga acatcacact
     7081 cagtcaactc acttagacga caacgactgc catagttatg gtggcatgaa tgatagattt
     7141 gattttattt taattgcagg ctcaataaaa gatggaagtg ctaatataaa atacaaaacc
     7201 gattcttaca aagcagttgg acaagatggt ttaagattta ataaaagttt aataagtcct
     7261 agtaacacaa gtttgccaca agaagttatt acagctcttt acgaaatgag cgaccaccta
     7321 ccagttgttg cagaatttta tatgggcgaa caaaatagtt ctatttcgtt aacaacagat
     7381 ccgcaatttt atgcaaatgt tgtaaatcct attgacgaca aacttgtttt taaattacaa
     7441 actatatata aaaacaaaat aatcaatctt agattaatga atatttctgg agctatagtt
     7501 tacgaaacgc aaattctaac tcaaactgac caaatttatt cttatgatat gacaggcttg
     7561 agcaaaggag tttatatttt aaattttaat ggcgaaggta taaaccaatc ggtaaaactt
     7621 gttaaaactg aataatctga aatgccaaag gactatagcc aattaattaa aaaattaata
     7681 agtttagaac cgaaaaaagt agatgattat tttcattctc ggcataaaaa agagtttgaa
     7741 aaaattaatt gcttagaatg tgcaaattgt tgcaggaatt taggtccttt gcttacagat
     7801 agcgatattg ccagattatc aaaacattta aaaattaaac cttcagaatt tacggaacaa
     7861 tatctgcgaa ttgatgaaga caacgattat gttttcaaga ccatgccttg tccattttta
     7921 caagatgata attattgttc agtttatgaa tttcgaccaa aagcatgtaa agaatatcca
     7981 catacagaca gaaagaatat tttaggaatt ttagatattt gcataaaaaa cacagaagtt
     8041 tgtccagcag tagcaaatat tttcaacaaa ctgaataacg acaaatttct aatgaaaact
     8101 taaacaataa taaatttgca aattcgttta taataatatt attttgtaaa aaatcttgtt
     8161 gatgaaaaaa cttgctacaa gcatattttt aatttttacg ataagttttt tatttgctca
     8221 atcgggcggc agaaacactt atgagttttt aaatttacct gcaggcgtta gaacagcagc
     8281 tttaggagga gttaatgttt ctcactatga tgatgatatt tctttcgtac ttagcaatcc
     8341 agcactgcta aaacctaata tgaatgaaaa agttactcta aattacataa actacattag
     8401 cgatataaac tttggatatg cttcatatgc tcgtgaattt aaaaatattg gaatgtttgc
     8461 tgctggtttg caatatataa attatggaaa ttttctttat gctgatgaat ttggagatat
     8521 tcacggagac ttttcagcag ctgaatatgc acttaacata agctacgcaa accaattgaa
     8581 ctcaaaaatt agttatggtg gaagttttaa agcaatttat tcagattttt atcaatactt
     8641 ttcgtcagga attgctttag acgcaggaat atcttatctc gacactgcaa aacttttctc
     8701 ggctggactt gtaataaaaa atgctggatt tcaactaaag ccttacacta aaggcaatca
     8761 agaaccttta cctttcgaca tacaaattgg agttacacaa aagctaaaac atgctccttt
     8821 tagattttca atcactgctc ataatttaac aagatggaat ttgcgttata aaagtatttt
     8881 cgacaatact tatgagttaa atacaagcga aaaccttgcc gacacgactt ttggtcaaaa
     8941 attaacaaaa tttatgaatg gtgttggcag agttggagat gagcttataa gacattttgt
     9001 tattggcgtt gaaatagttc ctacaaataa tttttatctt gcagtaagtt ataattaccg
     9061 ccgccgaagc gaaatgcgaa tcgaaacaag cccaaaaatt gttggacttt catttggtgc
     9121 aggaattaaa ctttcaaaat ttaatatctc ttatgcctta gcttcttatc acttggctgg
     9181 catgtcacac cacattagtt tgggattaaa tttgtctgaa ttttataaaa gccaaaagtt
     9241 ataaaactta actttataaa cttttagctg attttgttgt gttgcccttg cgggcaaggc
     9301 ttttgagggt ctatctaccg caggctgcgc ctacggttaa tcacatgttg tcctacggac
     9361 aagcctttga tagtcttgtt ttgtcctacg gacaagccac acctgctcgc tccaacaccc
     9421 ctgatgctct ctccaacagc catgacgctc gctcggattt gcaatccgag tgcttggtaa
     9481 tgttacactc ggattacaaa tccgagcgag caggttgtgg gaaaatccga gcaagcagag
     9541 ttagttgttt tacggacaaa taaatatgga tatgctgttt caattttgca actcccaaaa
     9601 acaataaatt acgcttgact atcaaagagt tagcgagtgc tacaaaaaaa gtacagaaga
     9661 caggaaaagt tataaaaatc aactttataa actttttact ttaaaaactt tacaaactca
     9721 aattatcttt tttgtgtctt atgctgattt cttcggctaa attttcaacc gcttttaaat
     9781 ctgcttctgt tggtgcgcct tttatataaa taggctcaat aacttcagct tttatatttg
     9841 gaatcattcc tgcaattttt tcaactgctc ttccacccca agcataagaa gcaagtatag
     9901 aaacatattt tgcttttgct tttagtgcat ttgctaaaat agttgcgtat gaaacaatag
     9961 gatgagcttc gcctaaaact gttggcgttc caattacaat agttgcagcg tcaaccagag
    10021 ttattgctaa ttttccaaga tcggttacag ttaaatcaat tttataagct ttaattccac
    10081 gattttctaa agcagtaact aaatgattta ccattttctc aacgcttccg tgcatagaaa
    10141 cataaggtat aatcacaaca tttttaggag tatcagataa ccattcttta taagcatcaa
    10201 ttataaattt tggattatca tacattggtc catggctagg agcaactatt tcaatatcgt
    10261 attcatcaag ttttggtaag tttttattaa taatgtttct aaaaggcaac attatttccg
    10321 cgaaatatct ttttgctgct tcatacacct cacattcgtt acttacaaaa atgtctgttg
    10381 ttgctaaatg cgaacctaaa aaatcacaag taaacaaaac tttatcttct tttaaataag
    10441 ccaacattgt ttcgggccaa tgcacccaag gaaaatgtat aaatttcaaa gttttgtttc
    10501 ccaaagaaat ttcttcgcca tcttctactg taataaatct atcttcagca attggcatat
    10561 gatcgctcag agtaggtttc gctttaggtg aacatataag tttagcattt ggatattttt
    10621 tcaaaacttc tggtagcagt ccagaatggt cttgctctga gtgcaaagaa actacataat
    10681 ctattttttt gattccttcg agtttttcaa aaaatatatg tgccaactca ggttctgcag
    10741 tatctattaa aacatttttt tcgctagcct caataaaata agcattgtaa cttgttccat
    10801 caggaagagg aattaaagca tcaaacaatc ttctattcca atcaactaca tcaagtaggt
    10861 ataagttctc tttaaatttt ctcgccataa tatttattat tttttaaaat ttttaatcat
    10921 gtacaaatat aataaaataa catcaaaaaa agaggttgtc tcaaaacaag tgttgaaccg
    10981 acaacctctc aaggtgtttc agaagtgtta aaatgcaagg cgctgaaatt gaggacgaag
    11041 gagcgtacat gaagcgttgc actgagcaaa gccgaagtgt acgtgactga gtccgaatat
    11101 tgaaagcaac gtggttgctg aacgaagttg aagtaaagca gtttgacact tatgagacac
    11161 cgcctttttt ataatatttt gaattaattt tctggaagca actgcacctt aacataattg
    11221 ttcaaatcga agtatttatt tgctgccttt ttcatagagc taatacttat tgattctatc
    11281 atatttaggt aatctttgta ttctgctgca ctaacattat tgtcttcaac atcaataata
    11341 agtcttttcc aataagaatt ttctttaacg tctgtttcaa attcgcgttt ttctttttca
    11401 ataacttttt gaatatcagt atctgtcaag ccgtcagttt gaagagattt tattatgtta
    11461 aaagttttag tttttaattc ctcttctctc tctggatcgc agctaaagaa aattgtaaca
    11521 gaatattgtt cgtaagggat tttagaactt gatggatacg cacctatagt ataaacacca
    11581 ctttcttttt cacgaatttc ttctaacaaa cgtgttgata aaattttaca cacagcatct
    11641 agttccaatc tattttgata tgtatattca aattcgcctg ggaagttaat tatttccata
    11701 cattttgggt ctttgccttt atataccgtt ttttcaacaa caccttttgg aggtcttact
    11761 cccaaatctt tataagtttc gcttctttca actattggta aagctcctaa atatttttcg
    11821 ataagaggtt ttgcaagttt agcatcaaaa tttccaacaa aatagaaagt gaagttactt
    11881 ggatctccaa atctttgtga ataaatactt tttactcttc tataattagc ttcttctaaa
    11941 acttcagcag ttagtaccct gcggcgtggg tgatttttag acattacagt tcttatacta
    12001 tcactccatg cagattgtgg atctaattta gcattttcta ataatccttt ttgcttgttc
    12061 atataagaat taaaagctgt ttcggtaact cttggttgag taaatgttgc ataaatcatt
    12121 tgcaataaaa cttcaaaatc tgcaacgtta gaagaaccta ctaaaccttc ggtactttca
    12181 tctatgtaag tactaaatct aacattttta tcagctaaga atttttgcaa ttcagattta
    12241 tcataattac ctaagccact ttcttcggca acatcatcag caattttact gctaatatca
    12301 tctttttggt cgtaaagaga atatccgcct aaacttcttg cttcaaacaa aatttcatca
    12361 tctttaaagt ttgttgtttt aaaaactact ttaacgccat ttttaagagt ccaagtttga
    12421 tagtcgaaat ctttattttt agttttctta ccaacttttg taggttttgc taaatctgaa
    12481 attaaggttt tatcagaaac tttatcaaca taagcatcta ctttaatttt agaggcatct
    12541 tgataaattt ttaggacttc gtcttcgctt ggaattgtaa ctccttcttt ttcgggagct
    12601 ataacaacga taacgcaatt ttcttctgtt acaacagttt ttgcaaaagc attaacttcg
    12661 tctaaagtaa tttgtggtaa atatttttta aataattcat actcatattc aattcctgga
    12721 aatggactat gtggtggtaa gaaatgtttt ttatattctt ctacataatt ttctgatttt
    12781 tgtttatctc tttcgttata agatttctct attcgtttta aaagagctgc tttttctctt
    12841 tctaattcag tttccgtaaa accaaattgc atcattcttt cattttcaac aactaaagtt
    12901 tctaaagtag tttttatgtc attattttga actattccca tgcttataaa aacatcttta
    12961 ggtcctataa actgtgaata tcctgcatag ccttgaacaa aaggaggatt ttctaaaagc
    13021 gttaattcag ccaaacgatt tgaaagcata gagctaagta acatattaac cataccgtct
    13081 ttataatcag caacagtaga atttggttga gcaggatgct tataaaacat ttgcaccata
    13141 ctaattggaa cttcggggtc tgttgctaca caaaccaaag tttctttatg atcagggatt
    13201 tctgcatata ctctttcgcg aggattatct atcattttaa ttttagaaaa tctttctata
    13261 actttttgtt ccatttcctt agcatcaaaa tcgccaacaa gaattaatgc catcaaatct
    13321 ggacgatacc aatctctata aaaacggcga atagcttcag gatcacagtt atcaactact
    13381 tccattgtac caattggcaa tctttttgca taaatagaat tatggaaaat agcttgtaaa
    13441 tatttacgtt gtacacgatc catagctcct tgtcccattc tccattcttc atgaataacg
    13501 ccacgttcag catcaatttc ttcagtttct aaagacaatt cagatgccca atcgtaaagc
    13561 actaataaac ctttatctat aaactcaggg tcatcagtag gaactttaat tccgtaaact
    13621 gtttcgtcaa aagaagtgta agcatttact tctggtccga atttcatacc taaagattct
    13681 aaataattta caagttcatt tttaggaaaa tttgtagaac cattaaaagc catatgctct
    13741 gtaaagtgag ccaatccttg ttggtcttca tcttctaaaa ctgagcctgc ttttacagct
    13801 agtgttaatt ctgctctatt ttctggttgc ttattagctt tgatataata agtcatacca
    13861 tttttcaaag ttcccgtaat tacacttggg tcatttggta acttgtcggt tagttccatg
    13921 ctctgaccaa aagcaaaact gcaactaaaa ataagtgcga taatcaaaaa tgtaagttgt
    13981 ttcatattaa atagctttat tttataatta ttttattaat tcaacaaaat taaaaatata
    14041 atttcagata ttttcatatt ttttagagaa atgcttaaaa aattgttaaa tggaatagtc
    14101 cttacctaca actctaatat tgcctcatta ttacaattat ctttaacaac aactttaatt
    14161 ttgtaagaat tggatttttc aaaatattca tctttttggt aaaaaattgt ttttgtttta
    14221 ggttcatatt gagtcaaaac ccacctatta ttaatataaa ttgcataaga agaaattcct
    14281 gacaaatcat ctgtaatttt aaaacttaaa taattcaaat tttcttctgt atatgttttt
    14341 ttaggtctta taggaacaat ttttggaggg gttgtatcaa tccacaaata aaattctcca
    14401 aaataagaag aataagcaga aataaaattt tgttcaataa ttggttttaa ataatttgat
    14461 tttttgttgt gttcacgcat gataaacaac ttgtctttat atttataagc ttcatcattt
    14521 atataaaaac ttattttaaa atccttattt acagccaaat gatcctctcc tataatataa
    14581 gattgcgaat attttctctt tttacctttt tctattttta agttagtatc ataaaacaaa
    14641 ctaccgattt gtgtttcaaa tctaaattct gaatcgaaat acaaaaagac gctatcgtat
    14701 tttacaagat acccgtctct ttcaagtaaa tcatagtttt tgttgtcttt aattaattca
    14761 aaatttaatt ctgaagaatt attattatag tcagtaataa taacagtaaa atttgctttc
    14821 gatttttcat ttaatgaaaa cattccatta ttaattgttt tatcataaat catcaattca
    14881 tttccagctt caacaaagag tttatgaaca tgaagttttt cttttaacaa atattcaaaa
    14941 tcaaacattg aatttttaga ggcttgagtt tcgaaagaaa ttttttctaa attagattta
    15001 taaattaatt cattatccat aaataaactg acggttttag ctccatatct attgcttgtt
    15061 tcgttcattc tatcaacata agccactcct aagccaatca gacctgttac ttcaggtaaa
    15121 tcgtttgcac aaacataaac tccatctctt ttcactactt tcaaaaaaaa cttctcagct
    15181 tttccattta taaaagaatt gtttgacaaa ggataaaaaa ctattgcaga aatttctggc
    15241 ggtaaattat ctttaaaatc atatacagaa tttaccacat tgattggctg ttctgtttta
    15301 gtttctctaa tttcaaaatg caaatgcgga ccttctgaat atcctgtatt cccactaaaa
    15361 gctacaagtt cacctttttt tactttaaat aaatctggat ttaaaagagt gtcaacagag
    15421 taagatttat tttcgtattg aatttttctt acaaaatttt gaatatcctt tctaaactca
    15481 ttcaaatgtc cataaacaga agtatagcca tttgggtgag ctatatacaa acctaaacca
    15541 taaccccaag gcgaaacaga aattctacaa acatatccat ctgcaacaga aacgatttct
    15601 ttgccatttt gataagttct aaaatcgatg ccagaatgaa aatgtctatg tcgtggttca
    15661 ccaaaattac cagacaaatt agtttcaaat tttaagggag aaacaaattc ttttttcaaa
    15721 ttaacatctt gagcaaaact caacaattgc aacaagaaca ggttaagtat tataaataaa
    15781 gacttcaatt ttaacatcat ctaaaaataa aaactttaca aaactaatat caattcttgc
    15841 tttttaaaaa taattagctt atttttgtag cgttaaataa aataatgtgg acgaagatta
    15901 taaagatata attatcaagc taaacgataa cattcagagt ttgatggctt cttattcaac
    15961 aataaaagag gataacatta ttttatctaa tgaggtaaat aaattgaata gtgaattaaa
    16021 tatatataaa gagaaattag aagaaataga aaacaagtat aataatttaa gattagcaag
    16081 aagtatagca gatggagata gtgatgataa agatgttaaa cttaaattaa acagaattat
    16141 acgggagatt gacaattgta ttgctctctt aaacaaatag ttctatgaat aacgaaaagt
    16201 taaaaataaa tataaacatt ggtgaaagaa catatcctat caatatttta agagctgata
    16261 gtaaacgcga ggagctaatt agaaaagctg ctcaactgat aaacgatgag ctttttcaat
    16321 ataaaagtag aggatataaa aaccgagatg aacaagacta tatggcaatg gttttgattt
    16381 caatttcagt tagactaatg gaaatggaag atcgtgaaga tgtttcacca gtaattaacg
    16441 aactaaaaaa aataaatttt acaatagaag attttttaga aaaagaaaga gagtaaacgt
    16501 tctttaaaat aacataataa taacaatact gcccgtattt gattctttaa aggttgaaaa
    16561 actcaacact aacaattttt gggataatgc tcggtttatc aacggcaggc ctaaacttat
    16621 tctgtttaca gaatattgtt gcaccagcga ccataggaaa ccgaaaattt actggcgatc
    16681 ccacccatgt tcatacaggg gtttttttaa cattaaagaa ttttatacgg gcttttttaa
    16741 tttaatactt aataatttta tgggaacatt aacaataatt ttagtcgcag taggagcttt
    16801 aattgcagga ggagcgattt cttactttat gtgggacaag gctttggaaa aaagaaaaaa
    16861 caaagtattt aacgatgcta tagcagaagc agaagttatt cgtaaagaaa aagaacttca
    16921 agccaaagaa aaatactttc aattaaagtc ggaacacgac aaaagaataa atgaaagaaa
    16981 tcaaaaaatg gctgctgccg aaaataaaat tcaacaacgc gaggcaataa ttgcgtcaag
    17041 aatagaagaa actcaaagaa tgaaaaagga gcttgaaatt attcgcgaaa accttgacaa
    17101 acaacttgat agagttaaca aaaggtcgga agaattagaa aagcttcatc gtgaacaagt
    17161 tgaaaaatta gaagcaattt ctggactttc tgccgaagac gcaaaagctg agctaatgga
    17221 atctttacta gcaaatgctc gttccgaagc tatgtcatta ataaatgaaa ttattgacga
    17281 ggctaaaatg actgctaata aagaagctaa aaaaatagtg ttagaaacta ttcaacgcgt
    17341 tgctacagaa acagctatcg aaaatgctgt tactgttttc aatatcgaaa gcgacgagct
    17401 taagggtaga attatcggta gagaaggcag aaatattaga gctttagaag ctgctacagg
    17461 tgttgaaata gttgttgacg acacgccaga agcaattttg ctatcagctt ttgaccctgt
    17521 tcgtagggaa gtagctcgtt tgtctttaca tcaattagtt acagatggac gaattcaccc
    17581 tgctcgtata gaagaagttg taaataaaac aaaaaaacaa atcgaagaag aaatagtaga
    17641 aattggaaaa cgaacaacta tcgacttagg tattcatgga ttgcaccccg aattagttag
    17701 aatggttggg aaaatgaaat accgttcttc gtatggtcaa aatttattac aacattcacg
    17761 cgaggttgca aatcttagtg ctcttatggc tgccgaactt ggcttaaacc caaaaattgc
    17821 aaaacgagcc ggtctattac acgacatagg aaaagttgct gatgaagagc ttgaattacc
    17881 acacgcaatt tacggaatga aattagccga aaaatacaag gaaaaacccg aaatttgtaa
    17941 tgctattggc gctcaccacg acgaaattga aatgacaagc ctcatctcac ctattgtgca
    18001 agtatgcgat gctatttcag gagcaagacc cggagcaaga agagaagtta tagaatctta
    18061 tattaaacgc ctaaaagatt tagaagcttt agctttatca tatccaggag ttttaaaaac
    18121 ctatgctata caagctggta gagaacttag agttatcgtt ggtagcgaaa aaattagcga
    18181 ctctgaagcc gaacaactaa gttttgaaat tgctaaacgc gttcaagacg aaatgactta
    18241 tcctggacaa gtaaaaatta cagttattag agaaactcgt tcagtaaatt atgcaaaata
    18301 ataagtttac aataaataat tctaaagtcc ttgttactgg cggtgccggt tttatagggt
    18361 ctaacattat tgaaaaatta ttagagcaaa acaattgtat agtttgcctt gataattttg
    18421 caacaggcaa acgcgaaaac ataaataaat ttgtaaaaaa caaaaacttt aaacttatag
    18481 atgacgacat cagagactta aatgcatgtc aaaaagcttg caaagacata gacattgttt
    18541 tgcatcatgc tgctctaggt tcagttcctc gttctatcaa tgaccctatt tcgagtaatg
    18601 aagcaaatgt taacggcttt ttaaacatgc ttgtcgccgc tagagacaac aatgttaaga
    18661 gatttgtcta tgcatcaagt tcatcagttt atggagataa tattgcttct cctaaaattg
    18721 aaaataaaac aggtaatgct ctttcgcctt atgcagttac aaagcaagta aacgaaatgt
    18781 atgctaaagt tttttctgat ttatacggct tagaaactat aggtttaaga tattttaatg
    18841 tttttggtaa aaatcaagat cctgaatctg tttatgcagc agtttttcca aaatttatta
    18901 aaagcttaat aaatttacaa ccaccgacaa ttcatggtga tggcactcaa tcgcgagatt
    18961 ttacctatat agcaaatgtt attcaagcaa acgaacttgc agctactacc aacaatccaa
    19021 aagcaataaa cacaatatat aatgttgctt atggagaaac taataactta aatgacatca
    19081 ctaatttgct tatacaatta ttagcaaaat tcaaacctga aattgccaat ataaaaccca
    19141 ttcacggacc tgaaagaaaa agcgatatta aagattcttt agcttcaatt gaaaaagcaa
    19201 aatctctatt aaactataat cctaaatttg atgtaagaca aggattagag ctgacagtaa
    19261 agtggtatta tgagaatttg aaataaaaaa agctgtccgc agacagcttt taatttttta
    19321 acacttaaaa ttggtttaaa tatctagaac agttacccat ccaaatgggt cttcgatatc
    19381 accatattgt attcctacta aagtttcgta taattttgta gaaactggtc ctgcttttcc
    19441 atctttacag aaagagtatg tttttccttc atcaaggtct ttaatgtctc ctattggtga
    19501 aattacagct gcagtgccac aagctccaac ttcttcgaaa gtactaagtt catcaaaagc
    19561 aactggtctt ctttcaacct tatatccaag atgagctgct aattgttcta aactcttatt
    19621 tgtaatagaa ggcaatattg atgtagattc tggtgtaata taagtattat ttctaatagc
    19681 aaagaagttt gctgcaccaa tttcatcaat atatttcttt tctttagcat ctaaatacat
    19741 tggtgaactg caaccagcgt ctttagctct ctttgttcct actaagcttg ctgcatagtt
    19801 accaccaact ttataagttc ctgttccaag aggagcagca cggtcagaat ctcttacaac
    19861 tgcgatgtcc acaggtttaa accctgcttt aaagtaagga cctactggcg taacaaagat
    19921 tataaaagtg tactcatcag ctggttgaac tccaactctt gctcccgttc caaacaatac
    19981 tggacggatg tacaaagatg cacctgttcc gtaaggtggc acaaagtctt tgtttaaagc
    20041 aataactttt ttcaacattt caaagaaaag ttcttcagga atttcagcca tttgaattcc
    20101 acgagcagaa tcttgtaatc gtcttgcatt ttctttccat ctaaaaagac gaattttacc
    20161 atcttttccg caataagctt tcattccttc aaaagctgct tgtccataat gcaatacagt
    20221 tgctgccata tgcatggtaa cgtgttctga atccgtaact tctatttcgc cccatttgcc
    20281 gtttttaaaa actgaacgga cattataatg ggtttttaca taaccaaaag gtaaatttgt
    20341 ccaatttaaa tcttgcatat tattaaattt ttatattcgc tcaaaattaa aaataaaaat
    20401 ctaaaattcc taaaaaaaca ttatgttttt aaatatattt ttaaaaattt atactttata
    20461 aattaataaa tatacgctat tgtctctatt attttaccat tcaacaagtt ctaagagtat
    20521 ttttttaatt tcagtattat caatagcacc accgaatcta tccgctccta ctccaactgc
    20581 aaacacaggc accgcagctg ctgtatgagc ccaagttgta aaaccaacac ctgcttttgt
    20641 acacattata tttccaaata ttgacgcaac tggattttct gcaccatata taattatagg
    20701 ttctaggctt gttgttggtg atttttcgta aaaataataa ttaaaagctt ccaaaactct
    20761 tgttgaatca gatgctgtat aaataggagt ttctgtaaag aaatattcat cagctaattt
    20821 ccaaatatca tcaacagaat attttgtatt attttcttta taatctttta ttttttcaga
    20881 aaaattagta atagatattt tttgattatc caagagcata aaattgcttt cataagccat
    20941 ttgtgcattt cctaatgaca agccacctgt ttcgtgatcg gcggttacaa aaatcaaagt
    21001 ttcttcagga tatttttcat aaaacttata agcttcgcga attgccaaat ccatatcata
    21061 aatttctttt acaattgttg ctccatcgtt tgaatgagct gcccaatcga ttttcccgcc
    21121 ttcaaccatc ataaaaaagc catttggatt ttccaaaact tttataccag ttttaacaaa
    21181 ttgagctaag ttgtatccgt catcactttt tctatcaatt ttatatggca catcgctttc
    21241 caaaccaagt tttggattga caaacatata ttttgtattt tttttatcta atttttcaaa
    21301 atcttttatg tttgagctta ctatataatt atttgacttt gctttttcat ataaatctgg
    21361 ctggttgtta tcgcttcctt ttacatgttt taatcctcct ccaccaaaaa attcaaagcc
    21421 agattctgga agctgacttc ctatttcata atataaatta cgacttgtag attgtgcgta
    21481 aaaagccgca ggagttgcat gatttagtga tacagtagta attattccaa ctttctttcc
    21541 gttttcgtga gctattttag caaatgacac gggaagcgaa tcctttgaaa accaaggata
    21601 atacgaaatg cttccaaaat tggctttttc tccacaagca atagccgagc cagcagctcc
    21661 cgaatctgta attacactat ttttacttgt tgttgtacaa actccaaaat atggaaaatt
    21721 agtaaaacta atatcatcgc ctttttcttt gtaaaaatat tttgccaagc tcgcatgatt
    21781 tattcccata ccatcgccaa taaataagaa aatatatttt ggttttttct tatcggctcc
    21841 aacattttcc ttattattca atgttttaca cgaaaaactt agtaaactca atactaagac
    21901 aatactaata acaaatacag atttaaaata tttcattttt cattttttta gaccacaatt
    21961 ttactcattt tttattaaat aaaaaaacaa attcaatgtt acaatggtgt taaaaataaa
    22021 cattattttt caacacatgt tatttcaaaa taatttatta tttttgggct taaattttat
    22081 gtttaaaaaa ttattatgca agaaaattta tttttataca attcgcttag tcgtaaaaaa
    22141 gaaaaattca cagctataaa acctccgttt gttggcatgt atgtatgtgg acctactgtt
    22201 tatggagatg ctcatttagg acatgcacgt cctgctatta cttttgattt actttttaga
    22261 tattttcaac atattggtta taaagtaaga tatgttcgca atattaccga cgttggacat
    22321 ttggaaagtg atgccgacca aggcgaagat aaaattgcta aaaaagcaaa attagaacaa
    22381 gttgagccaa tggaaattgc ccagttttat agcgacagat accacagaga tatggacaag
    22441 ctaaatgttt tgagaccaag catagaacct cgcgcttctg gacatattat tgaacaaatt
    22501 gaacttaccc aaaaaattat agatgcaggt tatgcttatg ttagcaatgg ttctgtatat
    22561 tttgatgttg aaaaatacaa tcaaaaatat aattacggta aactttcagg tagaatttta
    22621 gaagatttat attcaaatac tcgtcagctt gatgggcaag atgaaaaacg caatagcttt
    22681 gactttgcct tatggaaaaa tgctagtccc gaacatatta tgcgttggaa ttcaccatgg
    22741 ggacaaggtt tcccgggctg gcacgcagaa tgttcggcaa tgggacataa atatttaggt
    22801 gaaaaatttg atattcatgg tggcggaatg gacttgcttt ttcctcatca cgaatgtgaa
    22861 attgctcaaa atacaatagc tgttggcgaa aactccatta attattgggt tcataataat
    22921 atgattacaa taaatggaca aaaaatggga aaatcattag gcaattttat tactttagat
    22981 gagcttttta ctggagcaaa caaagtttta gaaaaagcct actcgccaat gacaattaga
    23041 tttttcattt tgcaagcaca ctatagaagt actttagatt tttcaaacga agctttacaa
    23101 gcagctgaaa aaggtttagc gagattacta aaaggtgttg aaacattaca aggattaaaa
    23161 ccaagcaaag aaaataattt tgatgcagaa aaattccgcc aagattgtta tgatgcaatg
    23221 aatgatgatt taaactctcc tattttaatt tctaatttat ttgaaggatt aaaaactatc
    23281 aattcagttg caacaggtag cgaaaaaatt gatgagaaaa atttaaacat cttaaaagaa
    23341 acatataaca cattcctatt caatgtttta ggattaaaat tagaaaatga agatgctggc
    23401 aataacgaaa tgttagaaga tgttgtaaac atggttttaa accttagact cacagcaaaa
    23461 gaaaagaaag attgggcaac ttccgacttt attcgtgatg aacttactaa aattggaata
    23521 aaagtaaaag atacaaaaga agggtttgaa tgggaaattt agttaagaat ctctaaaaat
    23581 attttgattt taaaataaat tttatcattt ttgaaaacta aataataaac atgtcaaccg
    23641 aaaataaaaa acccgataaa ctattctact ccattggcga ggttgccgaa atgtttaatg
    23701 ttaaaacatc tttaataaga tattgggaaa atgaatttga tattataaaa cctcaaaaaa
    23761 ataaaaaagg gaatagatta tttacagcaa aagatattga taattttcat ctgatttggc
    23821 acttggtcaa agaaagaggt atgacactta aaggtgcaaa aaagaagatt aaagaaaacc
    23881 ttgctgaaac agaacataat tttgaagctg taaaaaaact taacgaaatt aaagatttat
    23941 tactcgctat aaaaaatgaa ttataatttt taatgaagat aaaagatatt attaatagta
    24001 ttgaagaaat tgctccacta tctttacaag aagaatacga caactctggt ttacaagttg
    24061 gaaatttaga aaaagaaatt tcaggaattt taataagcct tgatgtaact tcagaagttg
    24121 ttcaagaagc aattgaccac aactgcaatt tcatcatagc tcatcaccca ctcattttca
    24181 acaggttaag aaaaattaca ggaagcgatg atatagaaaa atcaattatc ttagcaatta
    24241 aaaacgatat ttcaatttat tgtactcaca caaattttga caaagtaaat caaggtgtta
    24301 gctataaaat ttgtgagaaa ataggcttaa aaaatttaaa gatactttct cctgaaaaaa
    24361 acattttaga aaaaattgca gtttttgttc caacttctca tgctgatatt gtaagaaatt
    24421 caatgtttga ggcaggtgct ggtcaaattg gaaattacga taactgtagt tacaatttgc
    24481 aaggcgaagg aagttttaga gcaagcaata attcaaatcc atttgttggt aaaattggcg
    24541 aaacacattt tgaaaaagaa gtacgcatag aaaccatcta tccaaaatac cttagaaata
    24601 aaatattaca agcaattttc aaaacacacc cttacgaaga agttgcttac gacatataca
    24661 aattagagaa tccacataat aatataggtt tgggaatgat tggcgaattg gaacaagaaa
    24721 ttgcagaaat agatttttta aaacttttaa aagaaaaatt taatagcaca ataataaaac
    24781 acagcgaatt tttaaataaa aaaataaaaa aagtagctgt gtgcggtggc agtggtgctt
    24841 ttttaatcaa tactgcaaaa agcagtaaag ctgatgtctt tgttagtggc gacattaaat
    24901 ttcatgatta ttttttagca gaaaaacaaa tattaatagc cgatattgga cattttgaaa
    24961 gtgaacaatt cacaaaagaa attttttatg atattattat gaaaaaaaat cctaactttg
    25021 cagtccgatt ttcggaaaaa aatataaatc cgattaaaac tttttaaaat ggcaaaagca
    25081 gaaaataaaa ttgacaaatc aacacaaagt gttacagaaa agcttaaaaa cttgtacaga
    25141 cttcaattaa ttgacactaa aatcgaccaa ataagaattt taagaggtga attaccttta
    25201 gaagtcaatg atttagaaga tgaaattgca ggtttagaaa cgcgaatagc aaattttaaa
    25261 aacgacatca ctaaacttaa tgaagatatt tcgtttagcg aaaatgcaaa attagatgcc
    25321 gaagctctta taaaaaaata cacagagcaa caaaacaacg tgcgtaataa cagagagttt
    25381 gactctttaa gcaaagaaat tgagtatcaa aatttagaaa ttgaactttg cgataagaaa
    25441 ataagagagt acaaacatga aattgagcaa aagactgcac aaatagaaga agctaatgaa
    25501 aaattggttg acaaacaaca acatttagag cagaaaaaac aagaattaga tactattatt
    25561 tctgaaacta aagtagatga agaaaaacaa aataaagaga gagaagaaat tgcagaaaac
    25621 atagatgcca gattattagc tgcttacagt cgtattagaa acaatgtttt aaataaatta
    25681 gcagttgtgt ctatagagcg tgatgcttgt ggaggttgtt ttaataaaat tacaccgcaa
    25741 agacaaattg acattaaatc aagtagaaaa attattcctt gcgaattttg tggtagaatt
    25801 ttagttgacc cagatatttt agaaaaataa agaaattaaa aacgaagatt actaaaaaca
    25861 atcttcgttt ttttttgttg ttaaaataaa ataatatata aattgtgatt gttaataaaa
    25921 tgttgataat tattaattag acttattatt aatgtattaa cggttttgta tttttgtacc
    25981 aaattaaaaa aagaattgtt aaaaaaacta aatattaaaa tttaaaaaat tatgagaaaa
    26041 atttctttac taagtgcttt attgttattt gttacaataa catttgcaca aacaaatttt
    26101 actgccactt ataacttggc tggtagtgac aacaatgtta cgtcatttac atacaatggc
    26161 gacacttatg agggtataac accgcaacca cttttaaaag taggtgtttc tacatcacct
    26221 agtaataata attacagagc tacagattgg cctcttggag caacaagtgg tagtgatgta
    26281 tttactggaa ctattgattt ggagaaatac ataggtttct ctatagaacc agttacaggt
    26341 tacactttta ccattacttc aattaaattt ggacttggtc gtagtgctac tggtcctaga
    26401 caatttgaat ggagaggtaa tgctgatggt tttgcaagta ctttaagcac ttacgaagca
    26461 ctaaacacaa atcttaccaa tgtagatggg gtattaactt gtctagatga aaatagtaac
    26521 tggttaaaca atactttaac accaggtgca gcttatcaaa atttatctac aactactgaa
    26581 tttaggttgt acgcatataa tagtgaaaag gttggtggaa ctgctggact acaaggaaac
    26641 ctaattattg aaggtacttt tgttgcaact ggaactacag tattaacaat taatagtcct
    26701 gcaaacaacg ctatagtaaa cgaagaccaa gttgaagtta gtttttctac aacaaacttt
    26761 gaactaggaa ctgacggaaa acttgaatat aaattaaatg gaggagctcc tgcttataca
    26821 actacaagcc catttacaat cactggttta gcagaaggcg aaaacactat agttttccaa
    26881 ttagttaaca tggacaatac agctttagac ccagctgtta cagtaacacg tactgttaca
    26941 tatgaagttc ctaacacaga tccaagttta acaattactt tacctgctga taatacaaca
    27001 gtaaatgtta acgaagttca agttacattt actacgacaa actttgaact aggagtagct
    27061 ggaaaacttg aatataaatt aaacggtgga actgctaatt atgtaactac aacagatcca
    27121 tttacaattt ctgatttagc agaaggcgaa aacactatag ttttccaact agttaacatg
    27181 gacaatacag ctttagaccc agtagtttct gtaacacgca ctgttactta tgaaatagtt
    27241 gtattagaac ctagtttaac aataagctct ccacaagatg gagctattat ttattctaat
    27301 gacattaata ttaatttcac agttcaagat tttgtacttg gtatagatgg taaattagct
    27361 tacaaattag atgaaggagc aattcaatat tatactggca caagcccact tactttaaca
    27421 ggcgtagcat atggtacgca tcaaatttat tttgaactag ttacaatggc agatgcttca
    27481 ttagatcctg cagttacaac tactgtaaac tttactaacc aagaacctct ccctggtgga
    27541 atggaaactt tcgataatgc aaacattggc actagctatg cagatggttc attcattgga
    27601 aacaacaata ttcaatggac ttacacacat tctagagatg aaggtgatta tgcaatcaat
    27661 ggcaaaggtt taatgttgcg cagaccttct gatagtagta ttgagtcaca aacattaagt
    27721 ggaggtattg cttcttttga agttaaattc cgcaaagcat ttgaaggtgc tagtgaaaga
    27781 ggcttagaat tatacatcaa tggtgaacta aaaggcacat ctgacttgat tcctggtgta
    27841 agcggagcaa acgcaacaat atatacattt agcgtagata atattaacgt tcctggtgat
    27901 tttgtaataa aaatcaagat tgcaggcacc accgaatata atcgtcaaat aacaattgat
    27961 gatatttctt ggactggcta taatagcaca gaaccatatt tagctataac ttctccaagc
    28021 aatggacaaa ttttctatga aaatacagtt agtgtaaatt acacaacaac aaactttact
    28081 gttggtactg atggaaaagc aaaatatatt attgatggtg cagctcctgt ttatacaact
    28141 aataatccta ttactctaac aaacttagca gatggcgaac atacttttag tttagaatta
    28201 gtaaatatgg ataatgaatc ttattctcca gcagtaattc gtacaattac ttttactacc
    28261 aatactgaag gtccagaatt tacagatatc tatgatattc aatacacatt agatgaaagc
    28321 ggtgattctc ctctaaaaaa tcaatatgtt tgggttaaag gtattgttgt tgcaaacttc
    28381 aatcctagca catatggaga aggatactat attcaacaag gaggaggtgc ttggaatgga
    28441 atttatgttg ccgaccttgt aaattctcca acagttggcg actctgttat aattgcaggt
    28501 aaagttgttg aatcttacgg actaacatct attaaagaac ttacatattt tgaagcattt
    28561 gcaactgacg gaatattagc tgctccagcg gaagttagca cagctgatgc tggtactgaa
    28621 gcttacgaat cttgtttagt taaagttatt gatgctgaat gtactcaagg tataattagt
    28681 tatggcgaat ggtatgttga cgatggttct ggcgaattac tagtaaaaga aaacggcgtt
    28741 tcttcaattc aaccagttgt tggaaaccac tatgatatag taggtgttat ttattatgga
    28801 tatagtaaat tctcattaca atatagaagc gatgctgaca ttgttattac aaatatcaac
    28861 gaagacttcg caaatagttt aagcctatat cctaacccag ctaatagctt tgttaacatt
    28921 tcttcagaat ttgaaattaa caatataaaa gttacaaata atcttggaca agttgtttta
    28981 gaacaaaatg ctaaatctaa caatgttaat ttgaatgttg aaaatttaga atctggcata
    29041 tattttatca atataaacag taatgacaaa aacacagttc taagatttat taaagaataa
    29101 aataaattta ctaacaaaaa ggaaccgctt taaggcggtt tcttttttta atatttacaa
    29161 ctatgaaatc aaaattattt atacaacttt ttgcctttag tttgctgatt tttgtaagtc
    29221 aatcatgcaa aaaatacgaa aacggtccag ctattagttt catatctgcc gaaaaacgta
    29281 ttgtaggaaa atatgaacta gaaacttata aaataaatgg tatagaaatt aaacttagcg
    29341 aaattggatt agataatttt gaaaaagaat atttcgaaga tggcaaaggt aaaaattatt
    29401 cgcgtcaatt caacaatttg attgaaactg agtttgaatg ggaattgaac aaagataaaa
    29461 ctcaaatcag agaaagagaa aaaggattaa acggcgaatg gggacaatgg aattcatttt
    29521 ataccattac aaaacttaca aaaactgaat tttggacttt aaacgaaaat tctgcagaac
    29581 aacatgaatt tcattattta aaaataaaat aaagatttgt tgtttatatt aataaaaaaa
    29641 attaattttg taataattat taactaataa aattgaatat tatgaaaaaa ctcacaatta
    29701 ttttaagcat tatagtagga gctactatgt tgttttcatc ttgtcgcaaa tacgaagaag
    29761 gtcctgcttt ttcattagca acaaaaacag ctagactttc tggcgaatgg cttttaaaag
    29821 aagtaactgt aaataatgtt gcacaagata tgtcaacaga aggacaaata agtatgactt
    29881 taaaaaaaga tcaaacagga tctatgaata taactttttt tggtgcaact ctttcagaac
    29941 ctatagaatg gaaatttagt gatgacaaac ttaatcttat gataaaagaa gttgatgata
    30001 atgaatggaa tatatcagaa ataataagac taactagtaa agaactttgg ttgagagatg
    30061 ttgatgataa cacaactact gttattaaat atgaaaaaaa ataaaccaaa taaaatgtaa
    30121 aaatttaagc ctgtccaata acgacaggct ttttttgttc taaaacattt agaatatcaa
    30181 gtaaatttta tatctttatg ctcataaaat ttttgtatta tgataactaa agaagaattt
    30241 caaaaactta ttttacaagg cattcctgaa gttttgccac cagcagcaaa ttatgaaaaa
    30301 aacataagcc acgctcctat caggaagcaa attttatcta ttgaagaaaa aaaactagca
    30361 ttaaaaaatg ctttgcgata ttttcctaaa aaatttcatg cagaattagc tccagaattc
    30421 aaacatgagt tagagactta cggtagaatt tatatgtacc gttttcgtcc taattacaaa
    30481 atccattctc gtgatattac ttggtttcct cataaaacta ttcaggcagc agcaattatg
    30541 ctaatgattt ctaacaattt agattatgct gtagcacaac atcctcacga actaattact
    30601 tacggtggaa atggtgctgt ttttcaaaac tgggctcaat acttactagc aatgaaatat
    30661 cttgccgaaa tgacagatga acaaacttta gtaatgtatt ctggacatcc tttgggattg
    30721 ttcccttctc ataaagatgc tcctagagtt gttgttacaa atggaatgat gataccaaat
    30781 tactcaaaac cagacgattg ggaaagattt aatgctcttg gtgttactca atatggacaa
    30841 atgactgctg gttcatatat gtatattggt ccacaaggaa ttgttcacgg cacaacaatt
    30901 acagttttaa atgctagcag aatgattagc aaacacaaag atgctaatga ttgtaaatta
    30961 tttgtaactt ctggcttggg aggtatgagt ggtgctcaac caaaagctgg aaatattgca
    31021 ggagttgtta ctatttgtgc agaaatcaat ccaaaagcgg cttacaaacg acacgaacaa
    31081 ggctgggtag atgaaattgt tgaaaatgca gatgctgcaa tagacttagc attaaatttt
    31141 caagcaaaaa aacaagctca ttcgattgca tatttaggaa acattgttga tttatgggaa
    31201 aaacttgccg aaagaaatat taaagttgat ttaggaagcg accaaacttc attgcataat
    31261 ccttgggcag gtggatatta tcctgtagga tatagttttg aagaagctaa taatatgatg
    31321 gcaaattcgc ctgaaaaatt taaagaggct gttcaagaat cattacgcag acaagtagct
    31381 gcaataaata aacttacaga caaaggaatg tatttctttg attatggaaa cgcattttta
    31441 ttagaaagtt ccagagctgg tgctgatatt atggctaaaa acggaattga tttcaaatat
    31501 ccttcttatg tccaagatat tatgggacca atgtgttttg attatggatt tggacctttc
    31561 cgttgggttt gtacttcgag cgaccccaaa gaactagaac ttactgataa tattgctatg
    31621 caagttcttg aagacattgc aaaaactgct ccaaaagaaa tttctcaaca aatgaaagat
    31681 aatattaaat ggataaaaga agccaaaatt aacaaacttg ttgttggctc tcaagccaga
    31741 atattatatg cagatgctga aggaagaata aaaattgcta aagcatttaa cgatgctatt
    31801 aaagaaggaa aattaaaaca tccagttgtg ttaggacgcg accatcacga tgtttctgga
    31861 acagactctc cttttagaga aacttcaaat atttatgacg gaagccaatt tactgccgat
    31921 atggcaattc acaatgtaat tggcgatgct tttagaggag caacttgggt ctctattcac
    31981 aatggaggtg gagttggctg gggagaagtt ataaatggtg gctttggaat ggtattagac
    32041 ggaacagcag atgctgaaag aagactaaaa atgatgcttt tgtgggatgt taataatgga
    32101 attagtcgta gaaattgggc tagaaacgaa ggtgctattt ttgctattaa gcgtgcaatg
    32161 aaagaatatc ctgacatgaa agttactctt ccaaattttg ctgatgatga attaatcaat
    32221 aaactatatt aaacttaaaa tttcaaaatt atgaaaaaaa ttgtgttcac tctttttatt
    32281 tttacaacta ttatggcaat atcttgtaag aacgacaaca aaccttcaca aaacgaagaa
    32341 aatattattg aatttaaaat tttagatgaa aacatcgacg aagcaattca aaaaattaaa
    32401 gaaaaaaata ctgataaaga aaagtttaga atagaaaaag gagttagaca agttgcagca
    32461 ttatggcaac aaaacgatgg tgatgcaact gagttcataa acttttgcgt tgataatttt
    32521 atttctgatc ctgaggaatt aaaagttgct ttcgataaac tagaaagaaa ttttgaaatt
    32581 ttatttggac attacaataa aatttccgta gaactaatgg aacctttgca tttagacatg
    32641 ggcgaaattg gctttattga taatgttatg ggtgcttatt ctccatctgc tcacttaacc
    32701 gaagatttat ttgagaataa attagccttt tatgttgctt taaatttccc tgcttacagt
    32761 ttaagtgaaa aaacaaaaaa tgcagacact tggtcgcgtt tagattgggc ttatgctaga
    32821 atgggtgatt tatttatttc aagaattcca gctgtcttat atcaaaagga agcagaagct
    32881 ttaactaacg ccgacactta tattgccgac tataatattt atatgggaaa tcttgtaaat
    32941 tctgaacaaa aaacattttt ccctaaagat atgaaactta ttactcactg gggtttgcga
    33001 gatgagttaa aagcaaatta taatatggat aatggtttcg aaaagcaaaa aatgatttat
    33061 aatgtaatgc ttagaatcat aaatcaagaa attccacaag aagtaattaa taaaaatgag
    33121 tttacttggg atcctattgc taacaagatt tataaagacg gaaaagaagc aagtttcaca
    33181 agcgaacctt ttacaagata tgagtacatg ttatacaatt ttaaagctct tagtgcaatg
    33241 gataaatata atcctgttta cccaactttt attgaaagag cttatgatga aggcatggag
    33301 cttagtcgtg aagaagtaga aaaacttttt gtgaatttta ttagctcacc tgaaataaaa
    33361 gatcttgcaa aacttattga aaaaagatta ggacgttctt tacagcctta cgacatttgg
    33421 tataatggtt ttaaatctgg aacaaacatt aacgaagaag aacttgacca aattacacga
    33481 agaaaatacc caaatacttc agcttttgaa aaagatattg caagaatttt aattgattta
    33541 ggttggacta gagataaagc aaattatata gctaataaaa taagtgttga tcctgcacgc
    33601 ggagcaggac atgcttgggg agctcaaatg aaaggagacc aagctcatct tagaacaaga
    33661 attggaagta aaggtatgga ctataaaggt tacaacattg ccattcacga acttggacat
    33721 aatgtagaac aaacaataac actttacgac atagattact acatgctcaa cggcgttcct
    33781 aatactgcgt ttactgaagc tgttgcattt ttattccaat caaaagattt aaaactttta
    33841 ggaaaagaag ctaatagccg aaacgaagaa tcagaagctt tagctgcttt agacaattgc
    33901 tggagtgctt tcgaaattat gggtgtttct ttagttgata tgtatgtgtg gcaatggctg
    33961 tatgaaaatc ctgacgcaaa tccaaaagaa ctaaaagaag ctgttgtgaa aattgctact
    34021 gatgtttgga ataaatatta ttatccagtt atgggaataa aagactctcc tattttggca
    34081 atttattctc acagcattac atcacctcta tatttagcaa actaccctgt tgggcattta
    34141 attgagttcc aagttgagca atatattaaa gacaaaaatt ttgccgaaga aattaccaga
    34201 gttttattac aaggaagaat aattcctcaa gcatggatga aaggtgcagt tggaacagaa
    34261 atttctggtc aaccaactct tgaggcagtt aaaaaagctt tgaagattgt atcataaaag
    34321 ttacaaaaag acaatgtctt ataaatgttt caaatgttgc tgacatgagg acatttataa
    34381 gacat
//
LOCUS       DAOPNX010000018        33558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_1716_length_33558_cov_6.571083, whole genome shotgun sequence.
ACCESSION   DAOPNX010000018 DAOPNX010000000
VERSION     DAOPNX010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 33558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33558
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1716_length_33558_cov_6.571083"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PKN53_03340"
     CDS             complement(<1..504)
                     /locus_tag="PKN53_03340"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOD88017.1"
                     /translation="MDIKSVLIDLDKTLWDFDKNSNETIEELFYKFEADKYCSFSDYI
                     KKYREINNQLWEDYRNAKLEKDTLIWSRYYLSLKHFGLDNKELAQEMGQQYLKLSPTK
                     TKLIPHSIEILEYLQKKYKIYLVTNGFTEVQYTKARNCKIEKYFDRIFTSEEVGYNKP
                     HQLFFDFV"
     gene            657..863
                     /locus_tag="PKN53_03345"
     CDS             657..863
                     /locus_tag="PKN53_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HOD88018.1"
                     /translation="MNRFKMNTFKKKLIFRIVLTVLGGVAGLLYWKYVGCASGACPIQ
                     SKWYLSSLYGLVLGYLISGFVYSS"
     gene            919..1662
                     /gene="trxA"
                     /locus_tag="PKN53_03350"
     CDS             919..1662
                     /gene="trxA"
                     /locus_tag="PKN53_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOD88019.1"
                     /translation="MKKTLNLLMILALSFAVLSCGSSNKNDEKKEMKEEKEVVKSAFD
                     QAIEGSIPVVVDFYADWCKPCQIQGPIIDDLAKELGEQIKVIKVNVDNEQALAARFEV
                     QSIPTIMIFKENRIVYKAVGVQNTTVLKNALGDLGVDISMPNIEKSEPKSSFDQAIAG
                     STPVLVDFYATWCKPCQIQAPIIDELERELDGQIKVIKVDVDRELELSQRYGIQSIPT
                     IMIFKENQIVFKALGVQNKETLMQAINNL"
     gene            1814..2815
                     /gene="holA"
                     /locus_tag="PKN53_03355"
     CDS             1814..2815
                     /gene="holA"
                     /locus_tag="PKN53_03355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOD88020.1"
                     /translation="MDFEQILADLKNKIYKPIYLFYGEESFFIDELTNYIQQNVLTET
                     EKAFNQDIVYGKDITATNLIELSRAFPMMANYRVVILKEAQGLKDFKNLEPYVQNPVN
                     STILVLSFKNTAKVDKRLKVFNLLSKNAVVFESKRLYENQVYPWIEKTLRENGLSIHT
                     EALRLFYESIGADLSRLSNEIKKLVITQEKGKQISKEDIAFNIGINRDYNIFELQKAI
                     GLKDKLKCYQILDYFSKDPRANPLFATVNRLFDYFTKIIKLHMLQDKSKQSVANAAGV
                     HPYFAEEYLIAMKNYPMSKLIQIVSYLRETDMKLKGVGAVSISDADLSKELLYKILH"
     gene            2821..3441
                     /locus_tag="PKN53_03360"
     CDS             2821..3441
                     /locus_tag="PKN53_03360"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOD88021.1"
                     /translation="MTSNIVLIIVIVTAIFTYGAWQREDIFRKFLLSPYEVVHRKRYY
                     QIITHGFLHANWAHLLINMLVFWSFGSVVIQYFDILWGDFKNLMFLFFYLSAIAVSAL
                     FSIIKHKDNYYYSAVGASGATSAVVFASIFFNPWNKVYFFGILPIPGIVFGAIYLIYS
                     YQMAKRGKDNIGHDAHFWGAVYGFVFPLVLKPELWEYFYLRLINFN"
     gene            3808..4305
                     /locus_tag="PKN53_03365"
     CDS             3808..4305
                     /locus_tag="PKN53_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOD88022.1"
                     /translation="MNKAVFLDRDGVINRERGRYTTNADEFIINDGIGEAVKLLKDND
                     FLVIVITNQGGIAKSLYTAEDVLEMHIKFCMYLAEFDTKIDDFYFCPHHHDYGKCLCR
                     KPGSLLFEKAIKIYDIDTENSFMIGDSERDIIAASKCGIKGIQIEPNENILEICKQII
                     GGFYG"
     gene            4298..5134
                     /locus_tag="PKN53_03370"
     CDS             4298..5134
                     /locus_tag="PKN53_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOD88023.1"
                     /translation="MAKYVSINGNIQLSEKAFISYDNRGLIFGDSFNFEMRGNSSKAF
                     FEDEYFEFFIKSLKSLQFKKSILHRKNSLFTDLELLLQKNRIYKGFKARIIVFRNDNI
                     SNSCSIIISVDALENEYYPDLSNGLTIDVYKDYVIPEYSFEFDVTPKFSEELLLHQKL
                     ETLDDFLLCDKYGNIIKSLYSSVFFVKNASLILPTKFVNNSSRIFVDIVIKLANNLKI
                     PVVNWDVKEKDLYDLDEVFLGDIVNGFKRVMAFKNKRYFYKTTGKFLEALNNKVKVNF
                     KP"
     gene            5149..7089
                     /locus_tag="PKN53_03375"
     CDS             5149..7089
                     /locus_tag="PKN53_03375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOD88024.1"
                     /translation="MDYEKLSKQDLIEEIQKLTSKNDMLSERLTSLANLFEITTIGLY
                     KTNTDGDILEVNDPLLKMLGYSSLEDIQKYNIVKYELVNKKDREQFIKTLEKNDTISG
                     LETIWKKKDGTLINIRESARAIKNENGEIICIEGTVENIDDQKQKERELIESEEKYRT
                     LIELLPNGFVIHKNSIIVYVNKAMCKTLKVKDKSELIGRNIYDFIHPKLHDIVQERIK
                     NTILHDKNVDTREELIVAANNEYLNVEVSSFAAKIDGEQHVITITKDITEHKRAEQEI
                     LKAKEKAEKLNLHREALLKSVPDIIVTVEKNGNIIEFYSNYKSFCKEPVPEDFINKNI
                     EDVFSKNLTSFFKENINKVLKNKSIESFTYSNASLLKAKKLFFDARLVYLNETTVLVV
                     IRDITERMELLNDLEIAKQKAEESERLKTAFLANMSHEVRTPMNAIVGFADLLKYDVD
                     ENEKHEYINIIQNSCSQLLTVLDDIIELSKIESGSMLKNVTSFNLTELLDEVYLYWHN
                     SVLKSPNVELIFNKTNSSNLLCVSDKVKIKQIFTNLISNALKYTETGKVEFGYKEINV
                     SEIEFYVSDTGVGICPENIEKIFNRFTQIDNKLTNKTKGAGIGLSICKAYSELLGGKI
                     TVESIENEGTTFYFTFPKFTFA"
     gene            complement(7094..7729)
                     /locus_tag="PKN53_03380"
     CDS             complement(7094..7729)
                     /locus_tag="PKN53_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88025.1"
                     /translation="MILIIVLLSLSYFSSCRKKTYNHFSGTIYNLSSNQGVEGAKVIL
                     EASKIVSGNLNNTFHKIAETQTDEEGKYLIEVEQELYIKFRIRVEKEGYHSYFTEFEP
                     SEQIADYIKNYPLAQVSYLKIKLKNNPPSYPDDLLKVKVEGINKLCTECCDENFVLFE
                     GKDIDTEFFCNTVGGDTVRVNFITTTSGSTNFSTRSYYCVPGETVEDYFYY"
     gene            7828..9285
                     /locus_tag="PKN53_03385"
     CDS             7828..9285
                     /locus_tag="PKN53_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOD88026.1"
                     /translation="MSILGNLEPKNVWNYFEEITKVPRPSKKEEKIIAFLLDFAKKNN
                     LEAKRDKIGNVLISKPASKGMENRKSVVLQSHVDMVCEANKDTVIDFDNDPITPYIDG
                     EWVKAKGTTLGADDGIGMAASMAILTDNSLVHGPIECLFTVDEETGLTGAFEIEEGFF
                     KSKILLNLDSEDEGEIFIGCAGGIDLVANFDYKKENVPQNHLAYKFVVNGLQGGHSGD
                     EIHKGFGNSNKIANRFLNIINEKFNARLSVFDGGNKRNAIPREAEFVFTIDKSHEQAL
                     KKEFEIFKKNIVNEIVLTEKNINLSLESAKTPEFVIDLKTQNNLINAVYACPHGVHAW
                     SPAIPGFVESSNNLASVKFIDNKIQVVTSQRSSVESGKKDINNMVKAVFVLAGADVKI
                     GDGYPGWAPNPKSEILTIAEESYERLFGKKPVVRAIHAGLECGLFLEKYPDLDMISFG
                     PTLRGVHSPDERIEIKTVKMWYEHLLDVLKNIPKA"
     assembly_gap    9681..9690
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9755..10942
                     /locus_tag="PKN53_03390"
     CDS             9755..10942
                     /locus_tag="PKN53_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD88027.1"
                     /translation="MDSLVKIYLRLFQETEVGKLRYLYSDIDWNERCIALIGAKGAGK
                     TTLLLQHIKMNFANKKEAALFVSLDNTWFAKHTIFDLADEFYMNGGTHLFLDEIHHYP
                     NWAIEIKNIYDSFPKLKIVFTGSSLLQIYKSTVDLSRRVVYEVLECLSFREFLNFEKV
                     GDFPVLSLEEILENHQEIAFQITENVKIIPLFKKYLKNGYYLFYREGLKKYERKLQEA
                     INNVIDVDISAIENIGFESRYKLKRLLATLAALVPYTPNITELAKTIDTNRNNTLKYL
                     TLLANAKMLNLVSYENKTIGDLTKPDKILLNNTNLSYLYGNTNIGSMRETFFVNQVSA
                     VENLVLARNGDFMVNDYIFEIGGKNKTYNQIKDIPNSFIVADNIEIGHKNKIPLWLFG
                     MLY"
     gene            11260..11838
                     /locus_tag="PKN53_03395"
     CDS             11260..11838
                     /locus_tag="PKN53_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88028.1"
                     /translation="MDNKFFTFIKPYLSFIDKGDFFRKPFSWLYIAFAVLNLILPFYI
                     LYTAIDNNIFDTPAKFIIVFILVWIVIAFASWVCFQLWWDRKNKVTETSVENSEFVAT
                     PVFAHFIQTFGEWIGTWIGIVGFFVALLATIILGSDAVYLTAAIGVPFIQSGIAFIIL
                     IPIYGFLIVVGSRFLAEMCRALASIANNTKKN"
     gene            complement(11997..12977)
                     /locus_tag="PKN53_03400"
     CDS             complement(11997..12977)
                     /locus_tag="PKN53_03400"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOD88029.1"
                     /translation="MEKLKTNYMGLEINSPLILSSSGLSKKTQNIITAEENGAGAIIL
                     KSLFEEQINFMTNATLYDSIDYPEAADYIKNYSRHNFLNEYLDLIKASVKAVKIPVIA
                     SINCTSDTEWEGFAKEIEKAGAHGLELNMNVFHFDYELTSAQIEQKYFEIIEKVQAST
                     KLPIAIKMGKTFTNLPYFIKQIKFRGIQNFVLFNRFYKPIINIDKMEMTNSSAISDET
                     DIKESLRSIAIVKGMMPDIEISASTGIHNANGAIQLLLAGASSLQLCSVLYKNGLNEF
                     KTINAGILKWMEEKGYNSIDDFKAKLSYSNIKDPSVYERFQFIKHISTIE"
     gene            complement(12983..13810)
                     /locus_tag="PKN53_03405"
     CDS             complement(12983..13810)
                     /locus_tag="PKN53_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOD88030.1"
                     /translation="MRKKLFLSLGLIGLIVGAIFVTNCSTVPLIGRKQVTLIPESELV
                     SMGATNYKGFLDTTPRSRDVKNTVMLEEVGYKISKAVEQYLRDNKLEKRIKDFKWEFA
                     LVESNVPNAWCMPGGKICFYTGILPYTQNADGMAVVMGHEIAHAVARHGNERMTQQLM
                     AAAGQMALSEFIKTKPAETKAIFLNVFSVGTTVGVLLPYSRKHEYEADRMGLIFMAMA
                     GYNPNEAVAFWSRMSKMGGQKPPEFLSTHPSDAKRIENMKKCLPEAMEYYKKATKKQ"
     gene            complement(13807..14715)
                     /locus_tag="PKN53_03410"
     CDS             complement(13807..14715)
                     /locus_tag="PKN53_03410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD88031.1"
                     /translation="MQFLNICIPCFNHNVSKLVEELYTQCINENIEFQIIIFEDGSYA
                     ESVKKNSLLIEKFNVKHIVSEKNVGRSAARNRLAKEAKKGKIIFLDADSRIYHSDFIK
                     TYKQYLEHKIVSGGRKYLPEQYQKTNALRYKYGLLKETKTPKERNKYPNTGFATNNFM
                     IDSEVFEIVQFNESLKNYGHEDSLFGYELLKNGIKILHIDNPVVDEDIEENSIFLHKT
                     EEGIKNLILIEKSQITDKEFINEIKITKIAKRLKKLKLIFVIRFFNSIFKNKIKRHLL
                     NSSNPNLFLFDFYKLSYYCTLKNKEQ"
     gene            complement(14716..15240)
                     /locus_tag="PKN53_03415"
     CDS             complement(14716..15240)
                     /locus_tag="PKN53_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88032.1"
                     /translation="MKFFKFKILIIVIIILSFACSNKTKDKTSIDTNEELNLEQMIEK
                     YSNYNFSNCDEFIKVGNQIIDTYIKTVDLAFDGDMHAFYELDKYPEFMAKLDTVADKF
                     YIDCPEKIDNWTKSVEKRTSLSNKKIMAMTSSEIDSISWDESVEEELNRQIEELEEDL
                     KRIADEETSELKID"
     gene            complement(15281..16138)
                     /locus_tag="PKN53_03420"
     CDS             complement(15281..16138)
                     /locus_tag="PKN53_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOD88033.1"
                     /translation="MQRIIVPIDFSEESIKGLELGIIYANKFNADLQMVFVQKKKTGR
                     FHIELRKQYDAAMEEFEDLLRKYKDKLHKDCDFSYIIKSGRVHEEVARQAEAFDDSII
                     ICSTNGESGFSDFIIGSNAYKIVQTADRPVITVTTEKYIGEVKRIVLPIDISKETREK
                     VSKTAEIAKAFNSEVHIVKVTSSTSEGIHNKLELYASQVKKFLTEQGIKFQSTLLVGD
                     NITDITIEYATAIDADLIAIMTEQTFSLSNFLLSSYAYQMLNTSPIPVLSITPKGLYR
                     VESVRTTGG"
     gene            16239..17303
                     /locus_tag="PKN53_03425"
     CDS             16239..17303
                     /locus_tag="PKN53_03425"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007144.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOD88034.1"
                     /translation="MKKSFNIIGLMSGTSLDGLDIALVKFKEKNNNWIFKIVKAETVN
                     YEPVLIQKFLTAHLMSALELKYLEKEFSEFCAKAVNNFTNDISEKFDLIASHGHTVFH
                     QPEKGMTLQIGDGELIAKRCGINVISDFRSGDVALGGQGAPLVPIGDELLFYDYDACV
                     NLGGFSNISFKNNNKKRVAFDICPVNIVLNHLCQKIGEKYDENGKIASENIVNKTLLA
                     ELNDLDYYFKKYPKSLGREWVEKEVFPILEKYLQTDSVENIISTFTIHIAFQISENIK
                     NFSKVLFTGGGAYNGFLIKKIEEFSSVNIVVPENKIVDFKEALIFAFLGLLRWQNKIN
                     CLASVTGAERDSCCGKISFA"
     gene            17365..18786
                     /locus_tag="PKN53_03430"
     CDS             17365..18786
                     /locus_tag="PKN53_03430"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOD88035.1"
                     /translation="MQNNKQIQLSEFYRKVYNSEPKKIEKITQAGSPRFYCRFYSEEQ
                     SILGVYSDNVIETETFLYYSEVFTKLNLNTPKVYAVSDDKTCYFIKDFGDDLLLNLLL
                     QSQKENNTISQQIIDYYKKSLTALVKMQVLAKEHIDFTKAYFIQKFDAQSILFDLNYF
                     KYYFLNSSEIVYNEKKLQDDFEKLANDLDSGGTKLFMFRDFQSRNILVKNNEVYFIDY
                     QGGRLGAPEYDLVSLLYQARAGLPENLKLELKDFYFSELSKHIDLDKEEFDDRFYKYA
                     YLRVLQTFGAYGLRGLIEKKQHFVDSIPFALENLKTLMTLHPLSDDFVELKRVLQELI
                     LSKKHEHKVFDEFTVQVFSFSYKKGLPIDISGNGGGFIFDCRGIHNPGRYAEYKDKTG
                     KDKEVIEFFKTKSDIDVFIENAKTTVNSTVENYIERGFNSLMISFGCTGGRHRSVYCA
                     EAMAKYLKEKYKVNVILRHREQE"
     gene            18852..19256
                     /locus_tag="PKN53_03435"
     CDS             18852..19256
                     /locus_tag="PKN53_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88036.1"
                     /translation="MRKIFLLAMFLLSICFIYAQDCNCDESNVVESELKFSDLSDFEK
                     AEAIEAMLAGDCYYKGIKLWGRVKFVDTYPDIEIQYVETYPDIKVKFVTTYPDACGKW
                     QVVDTYPDFTVKVVDTYPDLRVKVVETYPGVD"
     gene            complement(19264..20241)
                     /locus_tag="PKN53_03440"
     CDS             complement(19264..20241)
                     /locus_tag="PKN53_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88037.1"
                     /translation="MKRILFIFTLCFFAFSLFSQSINTKAIERTNNINANYKTVVGTK
                     VKLIPPRGFIESNSYTGFQHELAGSSIIISEIPGEVNRNMIGLDKKYLIKTGVVVDKQ
                     TFYKINGFDAMLIEGQQVAYGKTYTKIMLLIGDIYRSYLISASMPSNVSKRHIEEVKE
                     SVLGVIYDPKIESQLSDRFDFSVDVSGTGLKKGNLMLSSLTYSDDGNVPSKTQEKTSM
                     TIRKSTISKALSEEEKIILCKRLFEAYPLEWDSKIKQDPKPFVAGNFKGYEMYAQGTH
                     KEMFVKEFIYQTIIFVNLDYYVITGFTYGKVEDNLKIFKKVAASLKPNK"
     gene            20300..21580
                     /locus_tag="PKN53_03445"
     CDS             20300..21580
                     /locus_tag="PKN53_03445"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HOD88038.1"
                     /translation="MPAKKINILCLVFFLLLSIPVFSIDKENIKCEDAVFEKNIKTVR
                     MHPIEWEQGYPLIEFGSDNILSFNFDQLESNPETYYYTILHCTYDWKSSNLMYFEYAT
                     GFEENVIDDYENSHNTFINYTHYNLEIPNRDVELTKSGNYLLIVYVKSGEETKVVITK
                     RFMLFENVLEIEARTGKALLNDYRLHFQKVDFTVYKKGLNIYDPQVELKPVVIQNYQW
                     NTAKYDFPFSFIDNDKIVYEWDDKIIFDAANEYRYFNFNNLELNSERVEHIEFRNPYY
                     YIDLIKDKTEMFTPYASVKDINGSYVIRTKRFKNSDYPEIQSEYAIVSFNLEHKNPIS
                     NGDVYIYGDLTNYSLDENSKMLYNIETRCYEKLLFLKQGYYNYRYVLVDKDGKINHSF
                     FEGSHQQTENAYLLFLYYKGMSDNYYRLINFLVI"
     gene            21902..22342
                     /gene="umuD"
                     /locus_tag="PKN53_03450"
     CDS             21902..22342
                     /gene="umuD"
                     /locus_tag="PKN53_03450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HOD88039.1"
                     /translation="MKKVFQTKRFEFYTAGIETKLELAYYGDIRAGFPSPAEDFNNER
                     IDLNKILIQNPLSTFYVKVIGNSMEGDFSDGDLLIIDKSLEWKENRIALCFVEGEFTL
                     KRIRIKNGKHYLVPSNSEFPIIEINEYNNATIWGVVAYSIKNHK"
     gene            22343..23599
                     /locus_tag="PKN53_03455"
     CDS             22343..23599
                     /locus_tag="PKN53_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HOD88040.1"
                     /translation="MFAHIDCNNFYVSCERVFNPSLNGKPVIVLSNNDGCAISRSNEA
                     KALGIPMGAPAFKYKEIIEENNVHVFSANFPLYGDMSNRVMSILATFCPMQEIYSIDE
                     CFLDLSGFDVDLKEYGLQMKERVYKWTGLPISIGIAPTKSLCKVATRVAKKFPKETNS
                     VHVIDSEELRIKALKWLEIGEVWGIGRQYTKKLLAKNIKNAYQFTCMGDDWVKKHMSI
                     VGLRLKHDLQGIPSIMIEEVKDKKNIATTRTFDYNTENYEDVKERIVTFTTKCAEKLR
                     KQGSCCNSIQVFIATNFYRKDLPQYSNSICIKLPFATNSTFELAEYAVVGLEQIFKKG
                     YAYKRAGVFVMDFTPENNQQLNLFQNRNAKHIPIMKAMDKLNRKYGCDLLRLGAQAPG
                     KTWRMKQEKLSPHYTTNINEVLTIKV"
     gene            23619..24236
                     /locus_tag="PKN53_03460"
     CDS             23619..24236
                     /locus_tag="PKN53_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOD88041.1"
                     /translation="MCFVYRLDKSANALEERFEAEFIHQQANIFFDNPNPIYNGFINP
                     KMPVITHSKPRNIQFFEWGLIPKWAKDKSFQKNTLNARIETLSEKPSFRDSVNHRCLI
                     PSDGFIEWRWLDEKGKNKQKYLVHFENDLLYAFAGIWSVWEDKPTGKLIPTFSIITTE
                     ANELMSFVHNNKKRMPVIIKSEFEKDWLETGNITMWNDDLKATAI"
     gene            complement(24261..25190)
                     /gene="fmt"
                     /locus_tag="PKN53_03465"
     CDS             complement(24261..25190)
                     /gene="fmt"
                     /locus_tag="PKN53_03465"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOD88042.1"
                     /translation="MNKLKIVYMGTPGFAVEPLKQLLEDNQKILAIITAPDKPAGRGK
                     KISESEVKTFAKDLNIKILQPTNLKYPDFISELKSLNADLFVVVAFRMLPKEVWQIPK
                     FGTINLHASLLPDYRGAAPINHAILNGDTETGLTTFFINEEIDTGNIIFQTKCEILES
                     DNAGTLHDKLMNLGGKLLVKTVNSIANGTAKSIEQDSLKTDKLRQAPKLNKEFCKLDF
                     NNSAEELHNKVRALSPYPAAWFELTNTENTIKVFETSYEINKHNLEIGKIISDNKNEI
                     KIAVKDGFLLIHELQMSGKKRMKTKDFLNGYKF"
     gene            complement(25183..26427)
                     /locus_tag="PKN53_03470"
     CDS             complement(25183..26427)
                     /locus_tag="PKN53_03470"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOD88043.1"
                     /translation="MSGNPQNEINRPLNTIEEAIEDIKNGKVVIVVDDYDRENEGDFV
                     VAAEKITPEIVNFMATHGRGLICAPLTESRCKELELELMVGKNTALHETPFTVSVDLN
                     GHGCTTGISAADRAKTIYALIDDKTKPEDLGRPGHIFPLMARENGVLKRAGHTEAVVD
                     LTRLAGLKVGGALVEIMNEDGTMARLPQLMKIADKFKLKIISIEDLIKYRLEFDSLII
                     RGDKVKLPTDYGNFEMIPFKQTNNGLEHSALIKGSWEPDEQILVRIHSSCVTGDIFAS
                     QRCDCGHQLHESMKMIEKAGKGVIVYLDQEGRGIGLMNKISAYKLQEKGLDTVEANLH
                     LGFKADERDYGVGAQILRSLGVRKMTLITNNPSKKAGLKGYGLEICAIQHIEIKPNKF
                     NKFYLKTKKDKMGHELNLGESE"
     gene            complement(26396..27562)
                     /locus_tag="PKN53_03475"
     CDS             complement(26396..27562)
                     /locus_tag="PKN53_03475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD88044.1"
                     /translation="MIIALQILFFFLAFLVFHSYVLFPFILYILTINKKLKFSELTEK
                     KEVTIIMSLYNEESVIAEKLDSILNSDYPLDKIKIIIGSDNSNDNTNEIVEAYAKKHQ
                     NIDFTAFTERQGKSNVINSMIDKSTSEILILSDANVFFDKNTISELVKPFSDPKVGLV
                     DSHMKNTGLKKEGISIQEKSYISREVYIKHREGILWGTMMGPFGGCFAMRRELYSPVP
                     KTFLVDDFYLCMKVLEKGYKAINNLDAVVYEDVSNNLSDEFRRKVRIATGNFQNLKEF
                     KHFLWPPWTGLAFSFLSHKVLRWITPIFLILAWGINFYLAFLSNFYLVLFCLYNFVLL
                     LPLIDFLLRKINIHNVFLRFITHFLSMNVALLTGMFRAMKGVKSNVWKPTKRNQ"
     gene            complement(27559..28770)
                     /locus_tag="PKN53_03480"
     CDS             complement(27559..28770)
                     /locus_tag="PKN53_03480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOD88045.1"
                     /translation="MRVLILTNKMPYPPRDGGSIASLSLALSLCQNKNHVEILSMNTS
                     KHYTELSSIPKEITNKVKIHAVKINTDTSIFKALTNLVFSSQPYNAQRFISKNFENKL
                     IEILEKDNFDIVQLEGLYLCPYIKTIRKHSNAKISLRAHNIEHEIWERATLNETNTCI
                     RIYKKNLAKRIKKFKLSYLNKYDLLVPITERDGKIFNELGNTKPQQVTPTGINQDNEF
                     LKLENNGEFPGFFHIGALDWIPNQEGLTWFIENVWNIFIKDNPDCKLTVAGRNAPVLF
                     EKYLRNCKNIEYLGEIPDAAKFIATQSVMIVPLLSGSGMRIKIIEGMAAGKTIISTSI
                     GSEGISTTHEKNILIANSSVEFLTSLNKIKNNRELHNSISLEARKFILENFDNDKIAQ
                     NLNDFYLLHIK"
     gene            complement(28818..30299)
                     /locus_tag="PKN53_03485"
     CDS             complement(28818..30299)
                     /locus_tag="PKN53_03485"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOD88046.1"
                     /translation="MLKKLHKYIIKSFLGPWLAIFLICVFILLLQFLWKYIDDLVGKG
                     LEWQVIAELLFYASLTLIPLALPLSILLATIMTFGNLGEKNELFAMKSGGISLYRIMY
                     PLFVLNLIISISAFYFSNNLLPYTNLKMRTLLYNIQQQRPEINLQSGIFTEPVNDFCI
                     KVERKQKKSNDLEDILVYDHRNKKNSSSITTAQHGNIHITKDKKFLILTLQDGYRYED
                     LDFDKDKSSKKYPHQSASFKTQTAYIELDALGMDQANEDLFKNSFDMLNNKQLVFSID
                     SLKKTMFERKNAIWFNIVEYSLFKGKHEWLNQSEEEKQIASRQVDELNVEEQQNIHVD
                     SIYKNLNSAQKNKVIDFALNYASSAKLYVELTRDELNYSNRYIARHRIEWHRKYVLAI
                     ACVLFYLVGAPLGAIIRKGGFGLPVIISVFLFLIYYVISITFEKTTREAVFEPSIGMW
                     MSTYVLIPLAGFLIYKAANDSMRVSTQFYSNLKSKFKRKSKKT"
     gene            complement(30347..30721)
                     /locus_tag="PKN53_03490"
     CDS             complement(30347..30721)
                     /locus_tag="PKN53_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HOD88047.1"
                     /translation="MTRIEIEYILNSSPRILFDRLSTETGLSEWFADEVFINDNIYTF
                     VWEGVEETAELLGIKDLDYIRFRWTDCENEEVYFEFKIQNHELTNEVSLIVTDFAIND
                     EREEVIELWETQINNLKYLIGA"
     gene            complement(30761..32680)
                     /locus_tag="PKN53_03495"
     CDS             complement(30761..32680)
                     /locus_tag="PKN53_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD88048.1"
                     /translation="MNKIILFVFLSVFCSLALFPQTYQLPNGGFEQWDDSSSDSEPSS
                     WNAFPSAQCDLSGMAALGCGTATATRHAKSTDTRPGSAGNYSCKIYATKISILGNTVI
                     ANGNITTGQIRIGSTTATNSENYNITRTNDSNFRQDLNAKPDSIVFWAKFVCPSESQQ
                     AKMIAVIHDNYSYRDPEGSDPNAASRVVGKSVKFFSRGNQAWKRYSVPFDYNYPASDA
                     KYILLTFTTNKEPGQGSESDALYIDDVEFIYNTTVSNILVNGNPIPNFSPNTYDYYID
                     ADCGSSNIVSATASSPNANITIEQANNTNLAKITVNNGNKTSIYKIHFYYSTTSYISD
                     EICVGNAYSNYGFELPVQNTAGTFNHTKIVFQSETCDSIINLSLKVNPTYNTEIDMMI
                     CENATYDFFGQTLSEEGIYQHSLSSIAGCDSTITLNLTIGSFYQTNINAKICEGETYT
                     EHGFNMNHAGVDTLFYVAQNNCDSLVVLNLSVYPNYHTTIKDTINKGENYKLNGFNLD
                     VFTEIGDFEFEETFISEHGCDSIVNLQLHVKELEINEEEEEEEEENNNDNTDFSFNIY
                     PIPAGETITIDSEKEVINQLNFIIYDMFGRQAMSGKLANTKTIIDTSELASGIYFIRI
                     TYPVDKTLTYKILKN"
     gene            32953..33369
                     /locus_tag="PKN53_03500"
     CDS             32953..33369
                     /locus_tag="PKN53_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOD88049.1"
                     /translation="MLVRRNTGGYLPSIFDAFFNEASGLESSTIFTKPEFNVYENEKN
                     FVIEAAVPGMDKKDFNIEVKDNVLEISSEREAKEEKKDEKYFYKGFCYGSFKKSYSLP
                     ENIDIDKINAGYENGILKITIPKDEQAKVLKQIKVG"
BASE COUNT        11560 a         4406 c         5276 g        12306 t
ORIGIN      
        1 aacaaaatca aaaaaaagct gatgtggttt attataacca acttcttcgg aagtgaaaat
       61 tctgtcgaaa tatttctcaa ttttacaatt tcttgcttta gtgtactgca cttctgtaaa
      121 gccattggtt acaagataaa ttttatattt tttctgtaaa tattccaaaa tttcaatgct
      181 gtgaggaata agttttgttt ttgttggact aagttttaaa tattgttgtc ccatttcttg
      241 tgcaagctct ttattatcaa gaccaaaatg ttttaaactt aaataataac gagaccaaat
      301 taaagtgtct ttttcaagtt ttgcatttct gtaatcttcc caaagctgat tgtttatttc
      361 tctgtatttc tttatatagt ccgaaaaact gcaatattta tctgcttcaa atttgtaaaa
      421 cagttcttca attgtttcgt ttgaattttt gtcaaaatcc cataaagttt tgtctaagtc
      481 aattaaaaca cttttaatat ccatactcaa ataataaatt gcaaaaatac ttatttagat
      541 ttttgctaaa tatgttttaa aaattttgtt tctttaaaaa atgttaattt caaattttaa
      601 gtatatattt gaaaacatat tttaaatata tttgtttaga aaacaaattt tgaagaatga
      661 atagatttaa aatgaataca tttaaaaaga aacttatttt tagaattgtg cttactgttt
      721 taggtggagt agcaggcttg ctttattgga aatatgttgg ttgtgcatca ggagcttgcc
      781 caattcagtc gaaatggtat ttaagttcgc tttatggatt ggtattaggc tatttaataa
      841 gtggttttgt ttattccagc taagaaaaaa gctgaactta atgaaaatga tataaataaa
      901 aaataaactt aatctaatat gaagaaaact cttaatttac taatgatttt agcactatct
      961 tttgccgtgc tatcttgtgg ttcatcaaat aaaaatgatg agaaaaaaga aatgaaagaa
     1021 gaaaaagaag tagtaaaatc agctttcgat caagctattg aaggaagtat tcctgtggta
     1081 gttgattttt atgctgactg gtgcaaacct tgtcaaattc aaggacctat tattgatgat
     1141 ttagcaaaag aattgggcga gcaaataaaa gttattaaag ttaatgttga caatgagcaa
     1201 gctttagcag ctagatttga agttcaatct attccaacaa taatgatttt taaagaaaat
     1261 agaattgttt ataaagcagt aggagtgcaa aataccacag ttttaaaaaa tgctttagga
     1321 gatttaggag ttgatattag catgcccaat atagaaaaat ctgaacccaa aagttctttc
     1381 gaccaagcca ttgcaggaag cactccagtt ttggtagatt tttatgccac ttggtgtaaa
     1441 ccttgccaaa ttcaagcacc tatcattgat gaattagaaa gagaattaga tggacaaata
     1501 aaagttataa aagttgatgt tgatagagaa ttggaacttt ctcaaagata tggaattcaa
     1561 tctattccta ctataatgat ttttaaagaa aatcaaatag tttttaaagc tcttggagtg
     1621 caaaataaag aaactttgat gcaagctata aataatttat agtactaact tgcattagtt
     1681 ttctttcttt aaacaatatc ttcctcattt ttagatattt attttataaa tctaataaaa
     1741 atttttaaag tttttgtttt tagattcaaa taacgataag tttttttatt tttgtaaaac
     1801 aaaaaattaa attatggatt ttgaacagat tttagctgat ttaaaaaata agatttacaa
     1861 gcctatttat ttattttatg gggaagaatc gttttttatt gatgaattga ctaattatat
     1921 acaacaaaat gttcttacag aaacagaaaa agcttttaat caagatatag tttacggaaa
     1981 agacataaca gctacaaacc ttatcgaact ttcgcgagct tttcccatga tggcaaatta
     2041 tagagttgtt atcttaaaag aagctcaagg tttaaaagat tttaaaaatt tggagcctta
     2101 tgttcaaaat ccagtaaatt caacaatttt ggtattgagt tttaaaaaca ccgctaaagt
     2161 cgataaaagg ttaaaagttt ttaatttatt aagtaaaaat gctgtagttt ttgaatcaaa
     2221 acgcttgtat gaaaatcaag tttacccttg gatagaaaaa actttaagag aaaatggctt
     2281 gagtattcat acagaagcat tacgtttgtt ttacgaaagt attggagctg atttgagcag
     2341 actttcaaac gaaattaaaa agctcgtaat aactcaagaa aaaggtaagc aaatttcaaa
     2401 agaagatatt gcttttaata tcggaataaa tagagattat aatatttttg aacttcaaaa
     2461 agcaattggt ttaaaagata aactaaaatg ttatcaaatt ttagattatt ttagtaaaga
     2521 tccaagagca aatccattat tcgcaacagt aaatcgactt tttgattatt ttaccaaaat
     2581 aataaaactt catatgttac aagataagtc aaagcaatct gttgcaaatg ctgcgggtgt
     2641 tcatccttat tttgctgagg aatatttaat agcgatgaaa aattatccaa tgtctaaatt
     2701 aattcagata gtttcgtatt tgcgagaaac tgatatgaaa ctaaaagggg tgggagcagt
     2761 atcaatttct gatgctgatt taagtaaaga acttttatat aaaattttac actagttcaa
     2821 atgacaagca atattgtatt aattatagtt atagtaacag caatcttcac ttatggagca
     2881 tggcaacgag aagatatttt tcgcaagttt ttactaagtc catacgaggt tgtgcatcgt
     2941 aagcggtatt accagattat aacacatggc tttttgcatg caaattgggc acatttatta
     3001 ataaatatgt tggttttttg gtcttttggt tctgtagtaa tacaatattt cgacatatta
     3061 tggggagact ttaaaaatct aatgtttctg tttttttatt tgtcggcaat agctgtttct
     3121 gctttgttta gtataattaa gcacaaagat aattattact attctgctgt tggagcttca
     3181 ggagcaactt cggcagttgt gtttgcaagt atatttttta atccatggaa taaagtctat
     3241 ttttttggaa tattgccaat accagggatt gtgtttggtg cgatatattt aatttattcc
     3301 tatcaaatgg cgaaaagagg caaagataat attggacatg acgctcattt ttggggagct
     3361 gtttatgggt ttgtgtttcc tttggttctt aaacctgaat tgtgggagta tttttatcta
     3421 cgattgatta attttaacta attatgaatt atgaattatg aattaagaat gaagaattaa
     3481 gaattgtgtt tcgacttcgc tcaacacccg atgattgaat gaagaatttt ggatttaaag
     3541 agtttttagt ttagtaggaa aataaatgaa gaattgagaa ttgtgtttcg acttcgctca
     3601 acacccgatg attgaatgaa gaattttgga tttaaagagt ttttagttta gtaggaaaat
     3661 aaatgaagaa ttgagaattg tgtttcgact tcgctcaaca cccgatgatt gaatgaagaa
     3721 ttttggattt aaagagtttt tagtttagta ggaaaataaa tgaagaattg agaattgaat
     3781 gaatgtgtta atataaaaat tagataaatg aataaagcgg tttttcttga tagagatggt
     3841 gtaattaatc gtgagcgtgg gcgttatact acaaatgctg atgaatttat tattaatgac
     3901 ggtattggtg aagctgtgaa attattgaaa gataatgatt ttttagttat agttattaca
     3961 aatcaaggag gtattgcaaa atctttatat acagctgaag atgttttaga aatgcacatt
     4021 aaattttgca tgtatttggc agaatttgat actaaaatag atgattttta tttttgtcct
     4081 caccatcatg actatgggaa atgcttgtgt cgtaaacctg ggtcgctgtt gtttgaaaaa
     4141 gccattaaga tttatgatat agataccgaa aattctttta tgataggaga tagtgagcga
     4201 gatattatag ctgcttctaa atgtggaatt aaaggaattc aaatagagcc aaacgaaaat
     4261 attttagaaa tttgcaaaca aattatagga ggattttatg gctaaatatg tttcaattaa
     4321 tggtaatatc caactttcag aaaaagcctt tatttcatac gacaatagag gactgatttt
     4381 tggtgattct tttaattttg aaatgcgtgg aaattcttca aaagcatttt tcgaagatga
     4441 atattttgag ttttttatta aatctttaaa aagtttgcaa tttaaaaaat cgattttaca
     4501 taggaaaaat agtttattta cagatctaga acttttatta caaaaaaaca gaatttacaa
     4561 aggctttaaa gcaagaataa tagtttttag aaacgacaat ataagtaact cttgttcgat
     4621 tataatatct gttgatgctc ttgaaaatga atattaccct gatctctcaa atggattgac
     4681 aatagatgtt tataaagatt atgtaattcc tgaatatagt tttgaatttg atgttactcc
     4741 aaaatttagc gaagaattat tattgcatca aaaactagaa actctcgatg attttttgct
     4801 ttgtgataaa tatggaaata ttattaaaag tttatattct tctgtatttt ttgtaaaaaa
     4861 tgctagctta attttaccaa caaaatttgt aaataattct agcagaatat ttgtagatat
     4921 tgtaataaaa cttgcaaata atttaaaaat tcctgtggta aattgggatg tgaaagaaaa
     4981 agatttatat gatttagacg aagtgttttt aggagatatt gtaaatggat ttaaaagagt
     5041 gatggcattt aaaaataaaa gatattttta taaaactaca ggaaaatttc tagaagcatt
     5101 aaataataaa gtaaaagtta attttaaacc ataaattaat tataaaaaat ggattacgaa
     5161 aaattaagta agcaggattt aattgaagaa attcaaaagc taaccagtaa aaacgatatg
     5221 ctaagcgagc gtcttactag cttggcaaat ttgtttgaga taacaacaat aggactttat
     5281 aaaacaaata cagatggcga tattttggaa gtaaatgatc ctttgctaaa aatgttaggc
     5341 tattcaagtt tagaagatat tcaaaaatac aatattgtaa aatatgagtt agtaaataaa
     5401 aaagatagag aacaatttat taaaacttta gaaaaaaatg atactataag tggtcttgaa
     5461 acaatttgga agaaaaaaga tggaacactt attaatataa gagagagcgc acgtgctatt
     5521 aagaatgaaa atggagaaat tatatgtata gaaggaacag ttgaaaatat tgacgatcaa
     5581 aaacaaaaag agcgagaact tattgaaagc gaagaaaaat atagaacctt gatagagtta
     5641 ttgcctaatg ggtttgtaat tcataaaaat tctataattg tttatgtaaa taaggcaatg
     5701 tgtaagacat taaaagtgaa agataaatca gagcttatag gtagaaatat ttatgatttt
     5761 atccatccaa aattgcatga tattgtgcaa gaaagaataa aaaatacaat tttacatgat
     5821 aaaaatgttg acactagaga agaattgata gttgcggcta ataacgaata cttaaatgtt
     5881 gaagtgtctt cttttgcagc aaaaatagat ggggaacaac acgtaataac aattacaaaa
     5941 gacattacag aacataaaag agcagaacaa gagattttaa aagcaaaaga aaaagcagaa
     6001 aaattgaatt tacatagaga agctttgtta aaatctgttc ctgatattat tgtaactgtt
     6061 gaaaaaaatg gaaatataat tgagttttac tctaattata aaagcttttg taaagagcct
     6121 gtgccagaag attttataaa taaaaatatc gaagatgttt tttcaaaaaa tttgacatct
     6181 ttctttaaag aaaatataaa taaagtttta aagaataaaa gtatagaaag ttttacatat
     6241 tctaatgcaa gtttgctaaa agcaaaaaag ttattttttg atgctcgtct agtttatttg
     6301 aatgaaacaa cagttttggt tgttattaga gatattacag agagaatgga gttgcttaat
     6361 gatttagaaa ttgctaagca aaaagcagag gaaagcgaac gtttgaaaac agctttctta
     6421 gccaatatga gccatgaggt tagaacacct atgaatgcaa ttgtaggatt tgcagattta
     6481 ctaaaatatg atgttgacga aaatgaaaaa catgaatata tcaatataat ccaaaatagt
     6541 tgttctcaac tgttaacagt tcttgatgat attattgaac tttcaaaaat tgagagtggc
     6601 tctatgttga aaaacgttac ttcttttaat ttaaccgaat tattagatga agtatattta
     6661 tattggcata atagtgtgtt aaaaagtcct aatgttgaat taatatttaa taaaactaat
     6721 tcctctaact tgctatgcgt gtcggataaa gttaaaataa aacaaatttt cacaaactta
     6781 attagcaatg ctttaaaata taccgagaca ggtaaagtgg aatttggcta caaagaaata
     6841 aatgttagcg aaattgagtt ttatgtttca gatacaggag taggaatttg tccagaaaat
     6901 attgaaaaga tttttaatcg ttttactcaa atagataata agttaacaaa taaaaccaaa
     6961 ggagccggaa taggcttatc tatttgtaaa gcatatagtg aacttttagg tggtaaaatt
     7021 actgttgagt ctatagaaaa tgaaggaact actttttatt ttacttttcc aaaatttact
     7081 ttcgcataaa tttttaataa taaaaatagt cttcaactgt ctcgcctgga acgcaataat
     7141 atgatcttgt tgaaaaatta gtgctacctg aagttgttgt gataaaattt actctaacag
     7201 tatctccacc aacggtattg cagaaaaatt cagtgtctat atcttttcct tcaaataaaa
     7261 cgaaattttc atcgcaacat tctgtgcaaa gtttattaat tccctcaact tttactttta
     7321 acaagtcgtc aggatatgaa ggaggattgt tttttaattt tatttttaaa tacgaaactt
     7381 gagcaagcgg ataatttttg atatagtcgg caatttgctc gcttggttca aattcagtaa
     7441 aataactgtg ataaccttct ttttcaactc taattctaaa ttttatgtaa agttcttgtt
     7501 ctacttcaat taaatatttt ccttcttcat ctgtttgcgt ttcagcaatt ttgtgaaaag
     7561 tattattcaa atttccagaa actatttttg atgcttctaa aataactttt gctccttcaa
     7621 caccctgatt tgaggataaa ttatatatcg ttcccgaaaa atgattgtaa gttttttttc
     7681 tacacgacga aaaatagctc agactcaata aaactattat taaaatcatg aaaattttat
     7741 attttgtctt aaatagagtt cgcatttatt aaaattttta aaaataattt tgtaatttta
     7801 gccaaaatta taaaaaatat taatattatg agtattttag gaaatttaga accaaaaaat
     7861 gtgtggaatt atttcgaaga aattacaaaa gttccacgtc catcaaaaaa agaagaaaaa
     7921 attattgcct tcttattaga ttttgcaaag aaaaataatt tagaggcaaa aagagacaaa
     7981 attggaaacg ttttgattag taagccagct tcaaaaggaa tggaaaatag aaaatctgta
     8041 gttttacaaa gccacgttga tatggtttgt gaagcaaata aagatacagt aattgacttt
     8101 gataatgatc caattactcc ttatattgat ggagagtggg ttaaagcaaa aggtacaact
     8161 cttggagctg acgacggtat cggtatggcg gcttctatgg caattcttac agacaatagt
     8221 ttggtgcatg gtccaataga gtgtttgttt acagttgatg aagaaactgg actaacaggt
     8281 gctttcgaaa ttgaagaagg ttttttcaaa tcaaaaatat tactaaatct cgattctgaa
     8341 gatgaaggcg aaatttttat tggttgtgca ggaggtattg atttagttgc aaattttgac
     8401 tataaaaaag aaaatgttcc acaaaatcac ctagcctata aatttgttgt aaatggtttg
     8461 caaggcggac atagtggaga tgaaattcat aaaggttttg gcaattcaaa taaaattgca
     8521 aacaggtttt taaatataat taacgaaaaa tttaatgctc gtctttctgt atttgacgga
     8581 ggaaacaaac gtaatgcaat tccacgcgaa gcagaatttg ttttcacaat tgataaatct
     8641 cacgaacaag ctttaaagaa agaatttgaa atatttaaga aaaatattgt taatgaaatt
     8701 gttttaacag aaaaaaacat aaatttaagt ttagagtctg caaaaacacc agagttcgta
     8761 attgatttaa aaacacaaaa caatttaatc aatgctgttt atgcttgtcc tcatggagtt
     8821 catgcttgga gtccagcaat tcctggtttt gttgaatctt ctaataattt agcttctgta
     8881 aaatttattg ataacaaaat tcaagttgtt acaagtcaac gcagttctgt agagtctggt
     8941 aaaaaagata ttaataatat ggttaaagct gtttttgttc ttgcaggtgc agatgttaaa
     9001 attggcgatg gttatcctgg ttgggcacca aacccaaaat cagaaatatt aactattgca
     9061 gaagaatctt acgaaagact ttttggaaag aagccagtag ttagagcaat tcatgcaggt
     9121 ttagaatgcg gtttgttttt agaaaaatat ccagatttag atatgatttc gtttggtcct
     9181 acacttagag gagtacattc tccagacgaa agaattgaaa tcaaaactgt aaaaatgtgg
     9241 tatgaacatt tactagatgt tttgaaaaat attccaaaag cataaaaagt ataatttgaa
     9301 gaattttaat attttgaaag ccaactgcct tgttgtgagg tggttggctt ttaagtttta
     9361 tgaaaaatat actctgtgtt aaattaaaag tgtttctttt atgaaacact tttatattgt
     9421 agttgtttta tagaagaaac aatttgcaaa aaaatccaaa tgtttacgca ttgcgtaaag
     9481 aattgacatg gacacattat tgattgttat tctaattata ttagtgcttt agttttatga
     9541 aaaatatagc tataatagat taaaagtgtt tctattaaga aacatttttg ttttataatt
     9601 gttttcaaaa agaaacataa aagatataaa aatgtttttt aaataaaaca aaaatactta
     9661 aaatttgcta tttttaaaaa nnnnnnnnnn gtactttaaa cttttatttc gagattcgta
     9721 attattttgt atctttgcga aaaacaaatt tattatggac agtttagtga aaatttattt
     9781 gcgtttattt caagaaacag aagtgggtaa acttcgttat ctttattcag atatagattg
     9841 gaatgagcgt tgtattgcct tgataggagc aaaaggcgct ggaaaaacta cgcttttatt
     9901 gcaacatata aaaatgaatt ttgcaaataa aaaagaagct gcactttttg taagtttaga
     9961 caatacttgg tttgctaagc acacgatttt tgacttggct gatgaatttt atatgaacgg
    10021 aggaacacat ttgtttttag atgaaattca tcattatcca aattgggcaa ttgaaataaa
    10081 aaatatttac gacagttttc caaaattgaa aattgtattt actggttcat cgcttttgca
    10141 aatttataaa tcaacggtag atttatcaag aagggtggtg tatgaagtgt tagaatgctt
    10201 atcttttaga gaatttttga attttgaaaa agtaggagat tttcctgtgc tttcgttaga
    10261 agaaattctt gaaaatcacc aagaaatagc attccaaatt acagaaaatg tgaaaataat
    10321 accattattt aaaaaatact taaaaaatgg ttattatctt ttttatagag agggacttaa
    10381 aaaatatgaa agaaaattac aagaagcaat aaataatgta attgatgtgg atatttcggc
    10441 aattgaaaat attggatttg aatctagata taaattgaaa agattattgg caactcttgc
    10501 agctttagtt ccttatactc caaatattac agaattagct aaaactatag atactaatag
    10561 aaacaatact cttaaatatt taacattatt ggcaaatgca aaaatgttaa atttagtatc
    10621 atatgaaaat aaaactatag gtgatttaac aaaaccagat aaaattttac ttaataatac
    10681 aaatttgtca tatttatacg gtaatacaaa tataggtagt atgagagaaa cattttttgt
    10741 aaatcaagtt agtgctgttg aaaatcttgt gcttgcacga aatggcgatt ttatggtaaa
    10801 cgattacata tttgaaattg gtggtaaaaa taaaacatac aatcaaatta aagacattcc
    10861 aaacagcttt atagttgctg ataatattga aataggacat aaaaataaaa ttccgctttg
    10921 gttattcgga atgctttatt aaaacaacga ataactctta attaatagga ctacattata
    10981 aaatctatat ttacattaaa ttctacataa ttatttttga tttttaaaaa taattttgta
    11041 tgtttgttga atatatccga tattaatcaa tttttttata gattgtggat aaattaagca
    11101 gatatatcaa gcaaatgagg gagcctccgg caactatttt ataattgtta aaatcttttt
    11161 tataaaataa taaacaaaag ttgagaccag aaacaacttt aataaacggg gcgaaatccg
    11221 aaaagcctta tgagtaggaa aaattaaaat tttaataaaa tggacaacaa atttttcaca
    11281 ttcattaagc cttatctatc ttttattgat aagggtgatt tttttcgtaa accattcagt
    11341 tggttgtata ttgcttttgc agttttaaat ctaattttac cattttacat tttatacact
    11401 gcaattgata ataatatttt tgacacacct gcaaaattta ttatcgtttt tatacttgta
    11461 tggatagtta ttgcttttgc atcatgggtt tgttttcaac tatggtggga tagaaaaaac
    11521 aaagtaacag aaacttctgt agaaaattct gagtttgttg caactccagt gtttgctcat
    11581 tttatacaaa cattcggaga atggataggt acttggattg gaatagtagg cttttttgtg
    11641 gcactattag ctacaataat attaggctca gatgcagttt acttaactgc tgcaattgga
    11701 gttcctttca ttcaatcagg tattgctttc ataattctta ttccaattta tggttttcta
    11761 attgttgtcg gctcaagatt tcttgcagaa atgtgtagag ccttggcttc aatagcaaat
    11821 aatacaaaga agaattaaca atctaatttt ttaataacaa atttgcaata tggtaagcta
    11881 tttttggcaa gctatgttgc aaatttgttg tttttataat aaatatttga aatttatatt
    11941 taggtagaat tattttagaa tttattgcaa cactatagtt tttgtaaact ttagttttac
    12001 tcaatagtgc ttatatgttt aataaattga aatctttcgt aaacgctagg gtctttaata
    12061 tttgagtatg aaagtttagc cttaaaatca tcaattgagt tataaccttt ttcttccatc
    12121 cattttagaa tacctgcatt tatggtttta aattcattta atccattttt atataaaaca
    12181 gaacatagtt gtaaacttga agcaccagca agtaaaagtt gaatagcccc atttgcattg
    12241 tgaattcctg tagaagccga tatttctatg tctggcatca ttccttttac tattgctatt
    12301 gaacgcaaag attctttaat gtctgtttca tcacttatag ctgatgaatt tgtcatttcc
    12361 attttgtcaa tattaataat aggtttgtaa aatctattaa ataagacaaa attttgtatg
    12421 cctctaaatt taatttgttt tataaaatat ggaagatttg taaaagtttt acccatttta
    12481 attgctatag gtaattttgt acttgcttga actttttcaa ttatctcaaa atatttttgt
    12541 tcaatttgag cagaagtaag ttcataatcg aaatgaaaaa cgttcatatt taactctaag
    12601 ccatgagcgc cggctttttc tatttctttt gcaaaccctt cccattctgt gtcggaagta
    12661 caatttatac ttgcaattac cggtatttta actgctttta cagaagcttt aattaaatct
    12721 aagtattcgt ttaaaaaatt atgtcttgaa taatttttaa tataatcagc agcttcggga
    12781 taatcaatgc tatcatacaa tgtggcattt gtcataaaat ttatttgttc ttcaaataaa
    12841 gattttaaaa ttatggctcc tgcgccattt tcttcggctg taataatatt ttgtgttttt
    12901 ttacttaatc cagagctgct taagattaag gggctgttta tttccaagcc catataattt
    12961 gttttaagtt tttccataat tattattgtt ttttagttgc ttttttataa tattccattg
    13021 cttcgggtaa gcattttttc atattttcaa ttcgttttgc gtcagaaggg tgagtgctta
    13081 aaaattcagg tggtttttga cctcccattt tagacattct tgaccaaaat gcaacggctt
    13141 cgtttggatt gtatccagcc atagccataa aaattaaacc cattctatct gcttcgtatt
    13201 catgtttgcg agaataaggt aaaagaacac caacagttgt tccaacgcta aatacgttta
    13261 aaaatattgc tttagtttca gcaggtttag tttttataaa ttcgcttaat gccatttgtc
    13321 cagcagctgc cattaattgt tgagtcattc tttcgtttcc atgtcgagct acagcatgtg
    13381 caatttcatg ccccattaca actgccatgc catcagcgtt ttgagtgtaa ggcaatattc
    13441 cagtgtaaaa acaaatttta ccaccaggca tacaccaagc atttggaaca ttagattcta
    13501 ctaaggcaaa ttcccattta aaatctttta ttcttttttc taatttatta tctctcaagt
    13561 attgctcaac agcttttgat attttatagc ctacttcttc aagcataaca gtgtttttta
    13621 catctcttga acgaggcgta gtgtctaaaa aacctttgta attggtagca cccatgctaa
    13681 caagttcaga ttcgggtatt aatgtaactt gttttctgcc aattagtgga actgtagaac
    13741 agttagttac aaatattgct cctacaatta aacctattaa gcctaagctt aaaaataatt
    13801 tttttctcat tgttctttgt tttttaatgt gcaataatat gataatttat aaaaatcaaa
    13861 caaaaataaa ttcggatttg atgaatttaa taaatgtcgt tttattttgt ttttaaaaat
    13921 agaattaaaa aaccgtatta cgaaaattaa tttaagtttt tttaatcttt ttgcgatttt
    13981 tgttatttta atttcgttaa taaactcttt atcagttatt tgtgattttt caattaaaat
    14041 taaattttta attccttctt cggttttatg aagaaaaata gagttctcct caatatcttc
    14101 atctacaaca gggttgtcaa tgtgtaaaat tttaattcca ttttttagta attcatatcc
    14161 aaataggctg tcttcgtgtc catagttttt tagcgattcg ttaaattgca ctatttcaaa
    14221 cacttcgcta tcaatcatga aattattggt agcaaaacct gtgtttggat atttatttct
    14281 ttctttagga gtttttgttt cttttagtaa accatattta tatcttagtg cattagtttt
    14341 ttgatattgt tcgggaagat attttctacc accactaaca attttatgct ctaaatattg
    14401 tttatatgtc ttaatgaaat cactatgata aattctagag tcagcatcta aaaaaatgat
    14461 tttacccttt ttagcttcct ttgctaatct gttacgtgct gcacttcttc caacattctt
    14521 ttcagaaact atatgtttta cattaaattt ttctattaat aatgaatttt ttttcacact
    14581 ttcagcatac gaaccgtctt caaaaattat aatttgaaat tcaatatttt catttataca
    14641 ttgagtatag agttcttcaa ctagttttga aacgttgtgg ttaaaacatg gaatacaaat
    14701 atttaagaat tgcatttaat ctatttttag ttcgctagtc tcttcgtctg ctattcgttt
    14761 taaatcttct tctaactctt ctatttgtct gttaagttct tcttcaacgc tttcatccca
    14821 agaaatgctg tcaatttcgc tactggtcat agccataatt tttttattgc ttaaacttgt
    14881 ccttttttca acagattttg tccaattatc tatcttttca gggcagtcta tgtagaattt
    14941 atctgctact gtatctaatt ttgccataaa ttctgggtat ttgtctaact cataaaaggc
    15001 atgcatatcg ccatcaaaag ctaaatcaac agttttaatg taagtgtcta tgatttggtt
    15061 accaacttta ataaattcat cgcaattact aaaattatag tttgaatatt tttcaatcat
    15121 ttgttctaag tttaattctt cgtttgtgtc aatgctagtt ttgtctttag ttttatttga
    15181 acaagcaaaa gataatataa taatgactat tattaatatt ttaaacttaa agaatttcat
    15241 taaattattt ttaagatgaa agaattaaga atattaaatt ttatccacct gttgttctta
    15301 cactttcaac gcgatacaat ccttttggtg taatactaag cactgggata ggcgatgtat
    15361 taagcatttg gtaagcatat gaacttaata aaaagtttga aagagaaaaa gtttgctcag
    15421 tcattattgc aattaggtcg gcatcaattg cggttgcgta ttcaatagta atgtcggtaa
    15481 tattatctcc aactaaaaga gttgattgaa attttattcc ttgttcagtt aaaaactttt
    15541 taacttgtga agcataaagt tcaagtttgt tatgtattcc ttcgctggta gagctagtta
    15601 ctttaacaat atgtacttcg gagttaaatg ctttagcaat ttcagctgtt ttagaaactt
    15661 tttctctagt ttctttgcta atatctattg gtaaaactat tcgttttact tcacctatat
    15721 atttttctgt agttacagtt attacaggtc tatctgctgt ttgaacaatt ttgtaagcat
    15781 tactacctat tataaaatca gaaaaacctg attcgccgtt tgttgagcaa ataataatgg
    15841 agtcatcaaa agcttctgct tgtctggcaa cttcttcatg aactcttccg cttttgataa
    15901 tatatgaaaa atcacaatct ttatgcaatt tatctttata ttttcttagc aagtcttcaa
    15961 actcttccat tgcagcatca tattgttttc ttaattcaat atgaaatctg ccagtttttt
    16021 tcttttgtac aaataccatt tgtaggtcgg cattgaattt atttgcataa ataataccga
    16081 gttctaaacc tttaattgat tcttcagaaa aatctatagg tacaattatt ctttgcatat
    16141 tcttaaagtt taattttgtt gctaagatat taaatatttt tcatatattt acttttttta
    16201 aaatattttt acacttcttt tgtgtttttt tattttctat gaaaaaaagt tttaatatta
    16261 ttggtttaat gtcgggtacc tcgctcgatg gtttagatat tgccttggtt aaatttaaag
    16321 agaaaaacaa taattggata ttcaaaattg taaaagctga aacagtaaat tatgaaccgg
    16381 ttttaatcca aaaattttta acggctcact tgatgtctgc tcttgagtta aaatatttag
    16441 aaaaagaatt tagtgagttt tgtgctaaag cagttaataa ttttacaaat gatatttcag
    16501 aaaaatttga tttaatagct tctcatggac atactgtttt tcatcagccc gaaaaaggaa
    16561 tgactttgca aataggcgat ggagaattga ttgctaaacg ctgtggcata aatgtaataa
    16621 gcgattttag aagtggtgat gtagctcttg gtggacaagg agcaccactt gttccaatag
    16681 gagatgagtt gttgttttac gactatgatg cttgtgtgaa tttaggagga ttttcaaata
    16741 tctcgtttaa aaataataat aaaaaacgtg ttgcttttga tatttgtcct gtaaatattg
    16801 ttctaaatca tttatgtcaa aaaatcggag aaaaatatga tgaaaatggt aaaatagcct
    16861 cagaaaatat tgtaaataaa actttattag cagaattaaa tgatttagat tattatttta
    16921 aaaaataccc aaaatcgctt ggaagagagt gggtagaaaa agaagtcttt ccgattttag
    16981 aaaagtattt gcaaacagat tcagttgaaa atataatttc tacttttaca attcacatag
    17041 cttttcaaat ttcagaaaat ataaaaaact tttcaaaagt tttgtttact ggtggcggag
    17101 cttacaatgg ttttttaatt aaaaaaatcg aggagttttc gtctgtaaat attgttgtgc
    17161 cagaaaataa aattgttgat tttaaagaag ctttaatatt tgcttttctt ggcttgttaa
    17221 gatggcaaaa taaaattaat tgcttggctt ctgttacagg tgcagaaagg gattcttgtt
    17281 gtggaaaaat atctttcgct taaatataac tatgtttttg tgaaatctta actaattttc
    17341 atagttttga aaaaaatatt tgatatgcaa aataataaac aaatacaatt gtctgaattt
    17401 taccgcaaag tttataattc agaacctaaa aaaatagaaa aaattactca ggctggttct
    17461 ccaagatttt attgtagatt ttattctgaa gagcaaagca ttcttggcgt ttattccgac
    17521 aatgttatag aaacagaaac ttttctttat tattcagaag tttttacaaa attaaatctt
    17581 aatacaccta aagtatatgc tgtttcagac gataaaactt gttattttat taaagatttt
    17641 ggggacgatt tattgttgaa tttattatta caatcgcaaa aagaaaataa tactatctct
    17701 caacaaatta tagattatta taaaaaatca ttaactgctt tggttaagat gcaagtttta
    17761 gctaaagagc atattgattt tacaaaagca tattttattc aaaaatttga tgctcaatca
    17821 attctttttg atttgaatta ttttaaatat tattttttga atagtagcga aatagtgtat
    17881 aatgagaaaa aactacaaga tgatttcgag aaattagcta atgatttaga tagtggaggt
    17941 acaaaacttt ttatgtttag agattttcag tccagaaata ttttagttaa aaataatgag
    18001 gtgtatttta ttgattatca gggtggaaga cttggtgctc ctgagtatga tttagtttct
    18061 ttgctgtatc aagctagagc aggtttgccc gaaaatctca aacttgaatt aaaagatttt
    18121 tatttttctg aattatcaaa gcatattgat ttagacaaag aagaatttga cgatagattt
    18181 tataaatatg cttacttacg tgtattgcaa acttttggtg cttatggctt acgaggttta
    18241 attgagaaaa aacaacattt tgtggatagt attccttttg cacttgaaaa tttaaaaact
    18301 ttaatgactt tacatcctct ttctgacgat tttgttgagt taaagagagt tttgcaagaa
    18361 ttgattttat caaaaaaaca tgaacataaa gtttttgatg aatttactgt gcaagttttt
    18421 agttttagct ataaaaaagg attacctata gatataagcg gtaatggagg cggttttatt
    18481 tttgattgta gaggaattca caatcctggg cgttatgccg aatataaaga taaaacagga
    18541 aaagataagg aagtaataga attttttaaa acaaaatcgg atattgatgt atttatagaa
    18601 aatgcaaaaa caacagtaaa ttcaactgtg gaaaattata ttgaaagagg ttttaattct
    18661 ttgatgatta gttttggttg tactggaggc aggcatcgta gtgtatattg tgctgaggca
    18721 atggcaaaat atctcaaaga aaaatataaa gttaatgtga ttttaagaca tagagaacag
    18781 gagtaatatt ttagttttag gaatgatttt tgcatagatt tttattaatt tttaatttaa
    18841 actttaaaat tatgagaaaa atatttttat tagcaatgtt tttattaagc atttgcttta
    18901 tttatgcaca agattgcaat tgcgatgaat caaatgttgt cgagtctgaa cttaaatttt
    18961 ctgatttaag cgattttgaa aaagccgaag ctatcgaagc catgttagca ggcgattgtt
    19021 attataaagg aattaaactt tggggaagag ttaagttcgt agatacatac cccgacatag
    19081 aaattcaata tgtagaaact tatcctgata ttaaggtaaa atttgtaaca acatatcctg
    19141 acgcttgtgg caaatggcaa gttgttgata cttacccaga ttttacagtt aaggttgtag
    19201 atacttatcc agatttaaga gtaaaagtag ttgaaaccta tccaggagtg gattaaaaaa
    19261 cttttatttg ttgggtttta agcttgctgc tactttttta aagattttta aattatcttc
    19321 aacttttccg taggtaaagc cagtaattac atagtaatct aaattaacga aaattattgt
    19381 ttgataaata aattctttaa caaacatttc tttgtgtgta ccttgtgcat acatttcgta
    19441 acctttaaaa ttgcctgcga caaaaggttt tgggtcttgt tttattttac tatcccattc
    19501 taatggataa gcttcgaaaa gacgtttgca taaaattatt ttttcttctt cactcaaagc
    19561 ttttgaaata gttgattttc taatagtcat agaagttttt tcttgtgttt ttgaaggcac
    19621 attaccatcg tcagaatagg taagagaact caacattaaa tttccttttt taagtcctgt
    19681 accacttaca tcaacgctaa aatcaaacct gtcgcttagt tgcgattcaa tttttgggtc
    19741 ataaataact cctaaaacag attctttaac ttcttcaata tgtcttttcg acacatttga
    19801 aggcattgaa gcagaaatta aataagacct ataaatatca cctataagta acattatttt
    19861 agtataagtt tttccgtaag ccacttgttg tccttcaatt aacattgcat caaagccgtt
    19921 tattttataa aaagtttgtt tgtcaacaac tactccagtt ttaattaaat attttttatc
    19981 aagaccaatc atatttctgt taacttctcc aggaatttcg cttataatta tgctactacc
    20041 agctaattcg tgctgaaaac cagtatatga attgctttca ataaaaccac gaggtggaat
    20101 taattttact tttgtaccaa ctactgtttt ataattggca ttgatattgt tggttctttc
    20161 tatggctttt gtatttatac tttgtgaaaa caaagaaaat gcaaaaaaac ataaagtaaa
    20221 tatgaataaa atacgtttca taattaaaat ttttgtaaaa ttaattaaat ttgttcaatt
    20281 attaaaaaat acaagtttta tgccagcaaa aaaaataaat attctttgtt tagttttctt
    20341 tttacttcta agtattcctg tgttttcaat tgataaagaa aatataaagt gtgaagatgc
    20401 tgtttttgaa aaaaatatta aaactgtaag aatgcatccg atagaatggg agcaaggcta
    20461 tccgcttatt gagtttggga gcgataatat actttcattt aattttgacc aacttgagtc
    20521 aaatccggaa acttattact atacgatact ccactgcact tatgattgga aatcctcaaa
    20581 tttaatgtat tttgagtatg ctactggatt tgaagaaaat gtaattgatg attatgaaaa
    20641 ctctcataat acttttataa attatactca ttataatctt gaaattccta atcgagatgt
    20701 agagttaaca aaatctggaa attacttatt gatagtttat gtgaaatctg gcgaagaaac
    20761 taaagttgta attactaaaa gatttatgct ttttgaaaat gttcttgaaa tagaagcacg
    20821 aaccggcaaa gccttattga atgattatcg tttgcatttt caaaaagtag attttacagt
    20881 ttataaaaaa ggtttaaata tttacgaccc tcaagtagaa ttaaagccag ttgttattca
    20941 gaactatcaa tggaatactg caaaatatga ttttccattt tcttttattg ataatgataa
    21001 aatagtatat gaatgggacg ataaaataat ttttgatgca gcaaatgagt atcgctattt
    21061 taattttaat aatttagaat taaattcaga aagggtagag catattgaat ttagaaatcc
    21121 atattattat attgatttaa taaaggataa aacagaaatg tttactcctt atgccagtgt
    21181 taaggatatc aatggttctt atgtaattcg tacaaaaaga tttaaaaatt ctgattatcc
    21241 cgaaattcag tctgaatatg ctatagttag ctttaatttg gaacacaaaa atcctattag
    21301 caatggcgat gtttatattt atggcgattt aaccaattat tcgcttgatg aaaatagcaa
    21361 aatgctttat aatattgaaa cacgatgtta tgaaaaatta ttattcttga aacaagggta
    21421 ttacaattat cgttatgttt tggttgataa agacggaaaa attaatcata gtttttttga
    21481 aggaagccat caacaaaccg aaaatgctta tttgttgttc ttatattata aaggtatgtc
    21541 tgataattat tataggttga taaatttttt ggtaatataa aataattaag aattaagaat
    21601 tgtgtttcga cttcgctcaa cacccgatga ttgaatgaag aattttggat ttaaagagtt
    21661 tttagttttg agtttttagt tggagagtat tgagtatgaa gtgaattaga aattcagctt
    21721 tgctgcattt ttattcgttt cttggcagag ttctagcaag tttggctcta cacttgctcc
    21781 ttagaaaatt aagatttttt tttatttgct tacgcatttt ttatgtaatt ttacgcttat
    21841 atattttcaa aaatttgaaa tcattgttta tctttacgga ataaatttct gaaatttgat
    21901 aatgaaaaaa gtctttcaaa caaagcgatt tgagttttat accgcaggaa ttgaaactaa
    21961 acttgaatta gcttattatg gggacattcg agctggtttt ccaagccctg ccgaagattt
    22021 taataatgaa agaattgatt tgaataaaat acttattcag aatcctttgt caacttttta
    22081 tgtgaaagta attggtaatt caatggaagg tgacttttct gatggtgatt tgttgattat
    22141 cgacaaaagt ttagaatgga aagaaaatcg tatagcacta tgttttgtag aaggtgaatt
    22201 tactctaaaa agaatacgta ttaaaaatgg taaacattac ttagttcctt ctaacagtga
    22261 gtttccaatt atagaaataa atgaatataa taatgcaaca atttggggtg tagttgctta
    22321 ttcaataaaa aatcataagt aaatgtttgc tcatattgac tgtaataatt tctatgtttc
    22381 ctgtgaacgt gtttttaacc cttcgttaaa tggaaaacct gtgattgtac tttcaaacaa
    22441 tgacggctgt gctatttcac gttcaaatga agcaaaagca ttaggtattc ctatgggtgc
    22501 gcctgctttt aaatacaaag aaattattga ggaaaataat gttcatgttt tttctgcaaa
    22561 ttttccactt tatggcgata tgagcaatag agttatgagc attttagcca ctttttgtcc
    22621 gatgcaggaa atttatagta tagatgaatg ttttttggac ttgtcaggtt ttgatgttga
    22681 tttgaaagaa tacggtttgc aaatgaaaga acgtgtttat aaatggacag gattgcctat
    22741 aagtattggt attgcaccaa caaaatcact ttgcaaagtg gcaacaagag tagcaaagaa
    22801 attcccaaaa gagacaaatt ctgttcatgt tattgatagc gaagaattaa gaattaaagc
    22861 attaaaatgg cttgaaattg gtgaggtttg gggtattgga cgacaataca caaaaaaact
    22921 tttagccaaa aatataaaga atgcttatca gtttacttgt atgggcgatg attgggttaa
    22981 aaaacacatg tcgattgttg gattgcgttt gaaacatgat ttacaaggta ttccttctat
    23041 aatgattgag gaagttaagg ataagaaaaa tattgcaaca actcgaacat ttgactataa
    23101 cactgaaaat tacgaagatg ttaaagagcg aatagttact tttactacaa aatgtgccga
    23161 aaaattaaga aagcaaggct cgtgctgtaa ttctattcaa gtgtttattg caactaattt
    23221 ttacagaaaa gatttaccgc agtattctaa ttctatttgt ataaagctac catttgcaac
    23281 taattcaact tttgaacttg cagaatatgc tgttgtggga ttagaacaaa tttttaaaaa
    23341 gggctatgct tataaacgag caggtgtttt tgttatggat tttactcctg aaaacaatca
    23401 acaattaaat ctttttcaaa atagaaatgc aaaacatatc cctataatga aagcaatgga
    23461 taaattaaac cgaaagtatg gctgtgattt gttgagattg ggagcacaag caccgggaaa
    23521 aacttggcga atgaaacaag aaaaactttc accacattat actactaata tcaatgaagt
    23581 gttaacaatt aaagtttgag agctatatat tttaaattat gtgttttgtt tataggttag
    23641 ataaaagtgc aaatgctttg gaagaacgct ttgaagcaga atttattcat caacaagcga
    23701 atattttttt tgataatccc aatcctatat ataacggttt tattaatcca aaaatgcctg
    23761 taataactca ctcaaaacct cgtaatattc aattttttga atggggctta atcccaaaat
    23821 gggcaaaaga taagagtttt cagaaaaata cattgaatgc gagaattgaa actttgtctg
    23881 aaaaaccttc atttagggat agtgtaaacc acagatgttt aattccttct gacggtttta
    23941 ttgagtggcg ttggttagat gaaaaaggaa aaaataaaca aaagtatttg gtgcattttg
    24001 aaaatgattt gttatatgct tttgccggaa tttggtcagt ttgggaagat aagccaacag
    24061 gcaaactaat tcctactttt tcaattatta caacagaagc caatgaactt atgtcatttg
    24121 ttcataacaa caaaaaacgt atgcctgtta taataaaatc tgaatttgaa aaagattggt
    24181 tagaaacagg aaatattaca atgtggaatg atgatttaaa ggcaacagcg atttgaaatt
    24241 atttcaaagc gtgaagtata ttagaattta tatccattta aaaagtcttt ggttttcatt
    24301 cttttttttc ccgacatttg aagttcgtgt ataagaagga agccatcttt tacagcaatt
    24361 ttaatttcgt ttttattatc gctaattatt tttcctattt caagattgtg tttatttatc
    24421 tcgtaagatg tttcaaaaac tttaattgta ttttctgtgt ttgttaattc aaaccaagct
    24481 gctggataag gcgataaggc tctaacttta ttgtgtaatt cttcggcaga attgttaaaa
    24541 tcaagtttgc aaaactcttt attcaatttt ggtgcttggc gtagtttatc tgtttttaga
    24601 gaatcttgtt caatactttt tgcagtaccg tttgctatag aatttactgt tttaaccaat
    24661 aatttgcctc ctaaattcat caatttatcg tgtaatgtgc cagcattgtc agattcaagt
    24721 atttcacatt ttgtttgaaa aattatattt ccagtatcaa tttcttcgtt aatgaaaaat
    24781 gttgttagtc cggtttctgt gtcgccgttt aaaatagcat gatttattgg tgctgcacca
    24841 cgataatcgg gtagaagaga ggcatgtaaa tttatagttc caaactttgg aatttgccac
    24901 acttctttag gtaacattct aaaagctaca acaacaaata agtcggcatt taaactttta
    24961 agttcagaaa taaaatcagg gtattttaaa tttgttggtt gtaatatttt gatattcaaa
    25021 tctttagcaa aagttttaac ttctgattct gatatttttt tgcctctacc agcaggttta
    25081 tcgggtgcag taattattgc aagaattttt tgattgtctt caagcaattg ttttaaaggc
    25141 tccacagcaa agcctggagt acccatataa acgattttaa gtttattcac tttcgcctaa
    25201 gttaagttcg tgtcccattt tatctttttt agttttaaga taaaatttgt tgaatttatt
    25261 tggttttatt tctatatgct gtattgcaca aatttctaag ccatatcctt ttaatcctgc
    25321 tttttttgat ggattatttg tgataagcgt catttttctt actccaaggc ttctgagtat
    25381 ttgtgctcca acgccataat ctctttcgtc agctttaaaa cctaggtgaa gatttgcttc
    25441 tacggtatct aaaccttttt cttgaagttt gtaagcagat attttattca taagtccaat
    25501 gccacgtcct tcttggtcga gatatactat cacgcctttt cctgcttttt caatcatttt
    25561 cattgattcg tgcagttgat gtccgcaatc gcaacgttga gaagcaaaaa tatcgccagt
    25621 aacgcaacta gaatgaattc ttactaatat ttgttcgtca ggttcccagc ttccttttat
    25681 aagagcagaa tgttctaatc cattgtttgt ttgcttaaaa ggaatcattt caaaattgcc
    25741 gtaatcggtt ggtagtttaa ctttgtcgcc acgaataatt aagctatcaa attctaagcg
    25801 atattttatt aaatcttcaa tagaaattat tttaagctta aacttatcgg caattttcat
    25861 tagctgtgga aggcgagcca tagttccgtc ttcgttcata atttcgacta aagcaccacc
    25921 aacttttaaa ccagccaatc ttgttaggtc aacaactgct tcggtgtgtc ctgcacgttt
    25981 tagtacgccg ttttctctag ccataagtgg aaaaatatga cctggtcgtc ctaaatcttc
    26041 aggttttgtt ttatcgtcaa ttaaagcgta aattgttttt gctctatcag cggctgaaat
    26101 tcctgttgtg caaccatgac cattcaaatc aacagaaaca gtaaatggcg tttcgtgtag
    26161 tgcagtattc ttgcctacca tcaactctaa ttctaattcc ttgcatctgc tttcggtaag
    26221 tggtgcgcaa attaagccac gtccatgtgt tgccatgaaa tttactattt ctggtgtaat
    26281 tttttcggct gcaacaacaa aatcgccttc attttctcta tcgtagtcgt caacaacaat
    26341 cacgactttt ccatttttaa tatcttcaat agcctcttct attgtgttta aaggtctatt
    26401 gatttcgttt tgtgggtttc cagacatttg atttaactcc tttcatcgct ctaaacattc
    26461 ctgtaagtag agcaacattc attgataaaa aatgtgtaat aaaacgcaaa aatacgttat
    26521 gaatatttat ttttctaagt aaaaagtcaa ttaatggtaa taaaagtaca aaattatata
    26581 aacagaataa tacaagatag aagtttgata aaaaagcaag gtaaaagttg attccccaag
    26641 ctaggattaa aaatattggt gtaatccatc ttaaaacttt atgagataaa aagctaaaag
    26701 ccaaacctgt ccaagggggc cacaagaaat gtttaaattc ttttagattt tggaaatttc
    26761 ctgttgctat tcttactttt cttctaaact cgtcagataa attatttgaa acatcttcat
    26821 aaactacagc atcgaggtta tttatagctt tgtatccttt ttctaaaact ttcatgcaga
    26881 gataaaaatc gtcaactaaa aaagttttag gaactggcga gtataattcg cgtctcattg
    26941 caaaacaacc gccaaaaggt cccatcatag ttccccataa aataccttct ctatgtttta
    27001 tataaacttc tcttgaaata tatgattttt cttgtataga tattccttct ttttttaatc
    27061 ctgtattttt catatgagaa tccaccaagc ctactttagg gtctgaaaaa ggtttaacta
    27121 attcggaaat agtgttttta tcgaaaaata catttgcgtc tgataaaatt aaaatttcag
    27181 aagttgattt gtctatcata ctatttataa cattagattt tccttgtctt tcagtgaatg
    27241 cagtaaaatc aatgttttga tgttttttag cgtaagcttc aacaatttcg ttagtgttgt
    27301 cgttgctgtt gtctgagcca attataattt taattttatc aagaggataa tctgaattta
    27361 atatagaatc gagtttttcg gcaattacgc tttcttcatt gtataaagac atgattattg
    27421 taacttcttt tttttcagta agttccgaaa attttagttt tttatttatc gttaaaatat
    27481 ataaaataaa tgggaatagt acatagctgt ggaaaactaa aaatgctaga aagaagaata
    27541 atatttgaag tgcaataatc attttatatg taacaagtaa aagtcgttta agttttgagc
    27601 aattttatca ttatcaaaat tttctagaat aaattttctc gcttctaaag aaattgaatt
    27661 atgaagttct ctattatttt ttattttatt taaagaagtt aaaaattcga ctgaggaatt
    27721 tgcaattaat atattttttt catgagtagt gctaattcct tcactaccta ttgatgttga
    27781 tataattgtt tttcctgcag ccattccttc aattatttta attctcattc cagagcctga
    27841 aagtagtggt actatcataa cagattgtgt tgcaataaat tttgcagcat ctggaatttc
    27901 gccaagatat tcaatatttt tgcaatttcg caaatatttt tcaaataaaa caggtgcatt
    27961 tctgcctgct acagttagtt tgcaatcagg attatcttta ataaatatat tccaaacatt
    28021 ttcaataaac catgttaatc cttcttgatt aggaatccaa tctaaagctc ctatatggaa
    28081 aaaacctgga aattcgccat tattttcaag ttttaaaaac tcattgtctt ggtttattcc
    28141 tgttggtgtt acttgttgtg gttttgtatt tccaagttca ttaaatattt ttccgtcacg
    28201 ctctgtgatt ggaactagta aatcatattt attcaaatat gaaagtttga attttttaat
    28261 tctttttgca agatttttct tataaatacg gatacaagtg tttgtttcgt ttaatgtagc
    28321 tctttcccaa atttcatgtt ctatattatg tgctctaagc gaaattttag cattagagtg
    28381 ttttcttatt gtttttatat atggacaaag gtatagccct tctaattgaa ctatgtcgaa
    28441 attatctttt tctaaaattt ctattaattt attttcaaaa tttttagaaa taaatctttg
    28501 agcgttgtat ggttgtgacg aaaaaacaag attagttaag gctttaaaaa tagatgtgtc
    28561 tgtattaatt ttaactgcat gaatttttac tttgtttgtt atttcttttg gaattgaact
    28621 taactcagta tagtgttttg atgtattcat tgaaagaatt tcgacatgat ttttgttttg
    28681 acataaagac agggctaatg ataaacttgc tatagaacct ccgtctcttg gcggataagg
    28741 cattttattt gttaaaatca aaactctcat atcgttacat aaaatcagta caaagtaata
    28801 aaaaattatt gagtttttta agtctttttt gattttcttt tgaatttgct ttttaaattt
    28861 gaataaaatt gtgtgctaac tctcatagaa tcgttagcag ccttgtatat taagaatccc
    28921 gctaagggga taagaacata ggtgctcatc cacattccta ttgaaggttc aaaaacagct
    28981 tctcgcgttg ttttttcaaa agtgatagaa ataacatagt aaattaaaaa taaaaaaact
    29041 gatataataa ccggtaatcc gaaaccacct ttacgaatta ttgctccgag aggtgcgcct
    29101 actaagtaaa ataaaacaca tgcaatagct aaaacatatt ttctatgcca ctctattcta
    29161 tgacgtgcta tgtaacggtt gctgtaattt agttcgtctc tagttagttc tacatatagt
    29221 ttagctgagc tagcataatt tagagcaaaa tctataactt tatttttttg agctgagttt
    29281 aaatttttat aaattgaatc aacatgaata ttttgttgct cttctacatt gagttcatca
    29341 acttgtctgc tagcaatttg tttttcttct tcactttgat ttagccattc gtgtttgcct
    29401 ttaaataatg aatattctac tatattgaac catatagcat tttttctttc aaacatagtt
    29461 tttttcaaag agtctataga aaaaacaagc tgtttgttat ttagcatatc gaaactattt
    29521 ttgaacaagt cctcattggc ttggtccatg cctagtgcat ctagttcaat ataagctgtt
    29581 tgtgttttaa aactagcact ttggtgtgga tattttttac ttgatttgtc tttatcaaaa
    29641 tctaaatctt cgtatctata tccgtcttgt aaagttaaaa ttagaaattt tttatctttg
    29701 gtaatgtgta tgtttccgtg ttgtgctgtt gtaatactac ttgaattttt tttgtttctg
    29761 tggtcatata caagaatgtc ttctaaatca ttgctttttt tttgttttcg ctcaacttta
    29821 atgcaaaaat catttacagg ttcagtaaaa attccacttt gaagatttat ttctggtctc
    29881 tgttgttgaa tattatagag caaggttcgc atttttaaat tagtgtaggg gagtaggttg
    29941 ttagaaaaat aaaatgcgga aatacttatt attaaattta agacaaaaag tggatacatt
    30001 attctgtata aagatattcc accagacttc atcgcaaata gttcgttttt ttctccaaga
    30061 tttccgaaag tcattatggt tgctaaaaga atagaaagag gaagtgctaa aggaattaat
    30121 gttaatgatg cgtaaaataa aagttctgca ataacctgcc attctaaacc ttttccgact
    30181 aaatcgtcaa tgtatttcca taaaaactga agaagtaata tgaatacaca aattagaaaa
    30241 atagccaacc atggacctaa aaaagatttg ataatatatt tatgtaattt tttaagcatg
    30301 tctaaaattt aaaccatgca aaaataaaaa acaaatttta aaagtcctat gctcctatga
    30361 gatatttaag attatttatt tgagtttccc aaagttcaat aacttcttca cgttcatcat
    30421 ttatagcaaa atcagtaaca ataagggaaa cctcatttgt taactcatgg ttttgaattt
    30481 taaattcaaa ataaacttct tcattttcgc aatctgtcca cctaaaacgg atatagtcta
    30541 aatctttgat acctagtagt tctgctgtct cttcaacacc ttcccaaaca aaagtataaa
    30601 tattgtcatt tataaaaacc tcgtcagcaa accattcaga caaaccagtt tcggttgata
    30661 atctatcaaa taatattctt ggcgaagagt taagaatata ttcaatttcg attcttgtca
    30721 taatgtatag ctttttatgt tttacatttg tgttgtaaaa ttagttcttc aatatcttgt
    30781 aagttagagt tttatcaact gggtaagtaa ttctaataaa ataaattcca gaggctaatt
    30841 cgctagtatc tataatggtt tttgtgtttg ctagttttcc gctcattgct tgtcgtccaa
    30901 acatatcata aattataaaa tttaattgat ttattacttc tttttcggag tctattgtta
    30961 tggtttcgcc tgctggaata ggataaatat taaaagaaaa atcagtatta tcattgttgt
    31021 tttcttcttc ctcttcttct tcttcttcgt ttatttcaag ttcttttaca tgaagttgta
    31081 agtttactat gctgtcgcag ccatgttcgc taataaatgt ttcttcaaat tcaaaatctc
    31141 ctatttctgt aaaaacatct aaattaaatc catttagctt atagttttct cctttattta
    31201 tagtgtcctt aatagtcgtg tggtaatttg gataaacact taaatttaaa actactaagc
    31261 tatcacaatt attttgagca acataaaaca aagtgtcaac tcctgcatga ttcatattaa
    31321 aaccatgttc ggtatatgtt tcgccttcac aaatttttgc gtttatatta gtttgataaa
    31381 aacttcctat tgtaaggttt aaagtaattg tgctatcaca cccagcaatt gaagataaac
    31441 tgtgctgata aattccttct tcgcttaatg tttgtccaaa gaaatcataa gtcgcatttt
    31501 cgcaaatcat catatcaatt tctgtgttgt atgttggatt aacttttagg cttaaattta
    31561 ttatactatc gcaagtttca gattggaata ctatttttgt gtgattaaaa gttccagcag
    31621 tgttttgaac aggaagctcg aaaccatagt ttgaataagc attacctaca catatttcat
    31681 ctgaaatata acttgttgtg ctataataaa aatgaatttt ataaatcgag gttttattcc
    31741 cattattaac tgtgatttta gcaagatttg tattgttggc ttgttctatt gtaatgtttg
    31801 cattagggct agaagcagtt gcagatacaa tattagaaga accacaatca gcatctatat
    31861 aatagtcata agtgtttgga gaaaagttag gtattggatt accgtttacc aaaatgtttg
    31921 agactgtagt attgtaaata aactctacat catctatgta taaagcatct gattcagagc
    31981 cttgaccagg ttctttgttt gttgtgaaag ttaataaaat gtatttcgca tcacttgccg
    32041 gatagttgta atcaaaagga actgaatatc ttttccatgc ttgatttcca cgactaaaga
    32101 attttacaga ttttccaacc acacgacttg ctgcgtttgg gtcagagcct tcggggtcgc
    32161 gatatgaata gttgtcgtga attactgcaa tcatttttgc ttgttgactt tctgagggac
    32221 aaacaaattt tgcccaaaag acaatagaat cgggtttagc attcaaatct tgtctgaaat
    32281 tagaatcgtt tgttcttgta atgttatagt tttcagaatt tgtcgcggtt gtgcttccaa
    32341 ttctaatttg accagtagta atatttccat ttgcaataac agtatttcct aaaatagaaa
    32401 tttttgttgc ataaatttta caactataat ttccagcact tccaggtcta gtatctgttg
    32461 attttgcatg tcgtgttgct gtagctgtac cacaaccaag tgcagccatg ccacttaagt
    32521 cacactgtgc tgaagggaaa gcattccatg aacttggctc gctatcagaa ctactgtcat
    32581 cccattgctc aaatccaccg tttggtagct gatacgtttg ggggaataat gctaatgagc
    32641 aaaatacgct caaaaaaaca aaaagtataa ttttattcat aagttcaatc acataaacat
    32701 acaaatatat aactttattt ttaaaattag tgtaaaaatg ctttaattta ttaaaaatta
    32761 ttcactttag aatgtttata aacaaaattg agacaagttg gcatgttttt atttgctgaa
    32821 tcggacaaat tgacattgtg aggtatgtgc ttactgaaaa atatttccga aatttagtaa
    32881 aggtatatta tttgattatt aaatagtaga aaataaatta aaatattaac ttaaaattat
    32941 ggaggaaaaa atatgttagt aagaagaaac acaggtggtt acttaccatc aatatttgac
    33001 gcttttttta atgaagcaag tggtttagaa agttcaacaa ttttcacaaa accggagttt
    33061 aatgtttatg aaaacgagaa aaattttgta atcgaggcag ctgttcctgg aatggacaag
    33121 aaagatttta acattgaagt aaaagacaat gttttagaaa tttcatcaga aagagaagct
    33181 aaagaagaga aaaaagatga aaaatatttc tacaaaggat tttgctacgg aagttttaag
    33241 aaatcttata gcttaccaga gaacattgat atagacaaga ttaatgcagg ttacgaaaat
    33301 ggaattttaa aaattacaat tcctaaagac gaacaagcaa aggttttaaa gcaaattaaa
    33361 gtaggctaat tttaaattat taaaaaaaag gcggtgtctc ataagtgtca aactgctgac
    33421 ctgagtttat aaagttaaaa gttcataaag tttttaaagt cggttttata acaaaatgtg
    33481 tcaactttta ttaggacgcg acacttttaa cacttcaact acgttcagtg catcgctttt
    33541 aacttttaac ttttaact
//
LOCUS       DAOPNX010000019        32464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_1793_length_32464_cov_6.569255, whole genome shotgun sequence.
ACCESSION   DAOPNX010000019 DAOPNX010000000
VERSION     DAOPNX010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 32464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32464
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1793_length_32464_cov_6.569255"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..1092
                     /locus_tag="PKN53_03505"
     CDS             67..1092
                     /locus_tag="PKN53_03505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOD88050.1"
                     /translation="MKRIILTILTISFALFAKNAVYSQDIHFSQFFSAPLFLNPSNTG
                     DFEGDYRAALNNKNQWNTFTNAYSSFAGSIDAGFDDVFISQSRSGIGLQINNDIAGDG
                     RFGTTQLYLNLAYQLNVDKTQKFRLGLGLAGGYVMHGINLNNLYFGNQYDGERFNENL
                     PSDEVLINQKMSYPDFGTGAKLKYKYNHKLNFELGFSAYHLLQPKRSFIENSEMFLPI
                     KYSTILFAEYQLKEDLFLEAHALHMVQQKHNEVDVGGIFRLDYNPLDLQSIYFGGLVR
                     AKDAGIIVFGFKYHNFKICFNYDINFSKLSTISRGKGGAEFSIIYVFFKPRPFQAPDY
                     KKCPDFI"
     gene            1074..3071
                     /locus_tag="PKN53_03510"
     CDS             1074..3071
                     /locus_tag="PKN53_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88051.1"
                     /translation="MSGFYLMKRFLIYIAVIFIGINSVAQTKEVVNLVVSGDKAFKEK
                     NWYGAAKLYEQALKSNQRMRDIVWKAAEAYRLDNDYVRAAKHYRFLAEKNPEKYPDAI
                     FYYAEMLKANEEFIKAQYNYRQFIAIKENDETNLKVKKSMQEILNCEKAWKMYNNPSG
                     IEIFQCDTNINSVYSDFSTAFYKDSILTFASIQPVGDSMKDYKSKIYQINFFENNSEA
                     ELFDETINKVDFDIANPHFSKDGKKMWFTVSESYQTGNTYIYSSEYVDNEWTEPQKLP
                     DVINRPGYNFTHPFMLERENENNILLFSSDQPGGEGGYDIYYVEVLPDDSWGVVRNLG
                     RPIVEDKTLQNFLDTSSIINTPGNEITPFYNIKDSCLYFSSDWYENLGGYDIFKVKGD
                     FTKWEKVESLEYPVNTAQNDTYYKIYPKQGLAFFTSNRKSAFAVEKQSCCNDLFYYEL
                     EKELPPELITEKKITTIITKTKLLVPITLYFHNDQPVPNSWDTVTSINYSTSYFDYLA
                     LQETYRKMYSKGLGKTEKLMAIDSIDYYFSYNVQDNYSKLLEFTSLMHELLIEGQKII
                     VTIKGYTSPLNTAAYNINLAKRRISSLVNYFAEYDNGIFLPYIESGQLEYEFVAFGKT
                     LSDGKVSDDPKDPRNSIYSPAASRERKIEIIAISVENLEQN"
     gene            3130..4347
                     /locus_tag="PKN53_03515"
     CDS             3130..4347
                     /locus_tag="PKN53_03515"
                     /inference="COORDINATES: protein motif:HMM:NF037962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HOD88052.1"
                     /translation="MTKFLEIIANLNWHGILQETLMITSFVVIVMLLIEFLNVLSQGK
                     LTKVISKKPGMQIITGTVLGLIPGCMGVFTAVSLYTHRLFTFGAMLAAMIATVGDEAY
                     FMLALMPKTAIFMFAILAVLAIVLGFVADKYLKLKNYAADKEFSFDIHDHDDCCDKPS
                     STFSFKNFSFLPKRMIILAIILVILGLTFGGVIGHTHSDKLIFSLPAANSVKKVEMSD
                     LNITHIHNHDCEHEHEHISETSHNENLHHNHEHGIDWVRVTILISAFFALIVVLFTKD
                     HFIKKHLWQHLIKKHLPKILIWIFVTLLAVNILLQFANFGNWIYDHLFIVLLIAIAVG
                     IIPQSGPHLVFVVLFLQGTIPFSILLANSIVQNGHGSLPLLAESKKSFVIMKTISITI
                     GLIVGISGLLFNF"
     gene            4587..5087
                     /locus_tag="PKN53_03520"
     CDS             4587..5087
                     /locus_tag="PKN53_03520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOD88053.1"
                     /translation="MSTQEFNTRLLDLEKSLRYFALSLTKDMETAKDLVQDTFVKAIQ
                     YRDKYTADNNIKAWLFTILRNTYLNQVTKLSAKNTINDETEDEYILKNTMYEEYNAEN
                     SINTKDIQSTIDNLEDDYRIPFQLFVDGYKYKEIADKTNLPIGTVKSRIFFARRQLME
                     NLESFK"
     gene            5269..6810
                     /locus_tag="PKN53_03525"
     CDS             5269..6810
                     /locus_tag="PKN53_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88054.1"
                     /translation="MSKYFLLLISLILPLTFFGQKHLRNANVALDKEQYAQAIQLYLT
                     QIAKSKNKPRIIAESSFKIGYCYKKLSQPEESALYFKKAIDLGYSDPVQYLYYGEALQ
                     MLQKYDSALIQYEIFKKLAPEHKLANKGIESIDFTFSSIENPTRHKVELFAILNSGEH
                     DYCPFYEVRNKHKIYFTSGRYAPTHVSISPESGEYCSNLFFSEQDKFGKWSKPVIVPG
                     LVNTVDEEGAAFLNRKSNNLYFTRCQYDKKKDKGCRIFVAKRVGSYWGQIKEVEILGI
                     PEHISIGHPAISDDELTLYFVADSLLGGLGGKDIYKVVRERKNAPFGYPQNLGHYINT
                     EADEIHPYIRSNGDLYFSSDGHPGMGGFDIFRAKHISGIKYEISNLGFPINSSRDDFG
                     IVFIGMKEEGFLSSTRTGGLGKSDLYYFLLPSLEFTLSGTIYDKITKEKMANVDIQVM
                     NDEYILMDSQQSDTNGSYEIDLLPENDYIIYYKLNGYRVAKEIVNTKTLTEAKHFLRD
                     VYIEK"
     gene            6856..8931
                     /locus_tag="PKN53_03530"
     CDS             6856..8931
                     /locus_tag="PKN53_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOD88055.1"
                     /translation="MKHIVFFILVMIIPVLLTAQSSLITKGDEYYNTYQYELAKDYYL
                     KAIPSLRTQEDKASVNYKLGICFKNLGDSDKSELYFQVAKNNYVKGVIKPDVLLFYAD
                     ALRMNGKYEDAIDVYKQYLQVSPQDHRAIAGMESCSIVPKWISRPTRYKVTNVSSFNS
                     QSFDFSPAWGSKDYRTLYFTTSREGTIGARSNYKTGQKFTDIFSVTQDRKGSWSEPTP
                     VAGGINSEDDEGACTVSNKGTEMYFTRCKAGKKVDEPCKIYYASKKGNVWGEAQLVNI
                     PGFENYEVGHPALSTDDKYLYFSGEAPTGYGGMDIYRSTIKANNAGHPINLGPIVNSL
                     GDEVFPTVRADGTLYFASDGHKGMGGYDIYKVVKDDKGNITDIENLKYPINSSFDDFG
                     IIFYGSEETGYLSSNRKGGKGGDDIYSFVLPPLSITLNGVVRDTTDMLKIRTLKDVKL
                     SLFNDDGLVADFLSSENGTFNYKLDVNKNYKIKADGGKDYFATSVQFTTMKIEYDTVI
                     NVVINLARIPRVIVLPNILYEYDKATLLPESTVSLDGLVKTLEDNPHIAIELRSHTDF
                     RGAADYNMDLSLRRAKSCVDYLISKGIKTDRLTAKGFGKSEPREITKELAKEFPYFKE
                     GDILSEDYINRLKDNKQKEDAHQLNRRTEFSVISTEYGKDKEQEKAKDEIPQGQPGSS
                     IIKDTGREF"
     gene            complement(9386..10243)
                     /locus_tag="PKN53_03535"
     CDS             complement(9386..10243)
                     /locus_tag="PKN53_03535"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOD88056.1"
                     /translation="MMNKIFIIGLIVLFFGSISFAQNNRQNDSLINFVDINNNKQGKW
                     EKKYPNGQVRYRGFFIDNLPTGVFTYYYQNGKIKSVLNYDESGGSKAELFWDNGNYAA
                     KGYFDSDNNKHLTWIMYFEDGKKSAVINFNHGVAEGNVLIFYQNIEKLALNCNYKAGK
                     LDGQYTKYFQSGAKIEEGTYKNGTRDGYWKFYSNSGMIDEEGMYVNGKKDGEWKIYRD
                     NPKGEIVNYDKGVPDNWDELMEEWRAKEEWAKENQNKFKQPEDYFDNPIEFFKPSTDP
                     NTQVEYKLK"
     gene            10460..11581
                     /gene="gcvT"
                     /locus_tag="PKN53_03540"
     CDS             10460..11581
                     /gene="gcvT"
                     /locus_tag="PKN53_03540"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOD88057.1"
                     /translation="MKTTAFTNYHIAKGAKMVEFAGHNMPIEFTGINDEHVTCREKLG
                     VFDVSHMGEFWVKGPKAVDFLQKVTSNNILDLTDGKIQYSCFPNDKGGIVDDFLVYRF
                     NAESYLLVVNAANIDKDWAWCTAIASKMGMKIGEDLINASDEYCQLAVQGPYAIKAMQ
                     KLTSANITDMEYYTFKEIPFAGIESVIFSITGYTGAGGCEIYAKNKDAEKLYNAVFEA
                     GEEFGIKSIGLGARDTLRLEMGFCLYGNDINETTSPIEAGLGWITKFVDGKDFVNREY
                     HEKLKAEGASKMLKPFEMIDRGIPRQHYLVYDAEGNEIGEVTSGTMSPSMKVGIGMAY
                     LNKGFWKVDTEIFIGVRDKRLKAKVVKAPIFKNQIQVVK"
     gene            11624..12235
                     /locus_tag="PKN53_03545"
     CDS             11624..12235
                     /locus_tag="PKN53_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOD88058.1"
                     /translation="MKIICIGHNYKDHIEEMNAVLPEKPVFFLKPDSSILQTGKDFYL
                     PDFSNDIQYELEIIVKIHRLGKHIDKKFAHRYYSHIGVGIDVTARDLQRDCKEKGLPW
                     EIAKAFDGSAMLSEWIPIEEIEDVNNINFKLMRNGELVQLGNTKNMVFNIDEIIEYVS
                     KFLTLKIGDIIFTGTPKGVGKLEIGDELKAFIEEKELLNIKIK"
     gene            12247..13254
                     /gene="pta"
                     /locus_tag="PKN53_03550"
     CDS             12247..13254
                     /gene="pta"
                     /locus_tag="PKN53_03550"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOD88059.1"
                     /translation="MDLLQKIKENARKHEMRIVLPEGTEIRTLKAANQIIEEKLAKII
                     LLGSPEVINKTASENNLTNIKNAIIIDPKNHDKKEAYTDLLVELRKSKGLTKEEASKL
                     VENPLYLATLMIKAGDVDGEVAGADNATGDVLRPAFQVVKTLPGISVVSGAFIMILND
                     KKFGEDGLMVFADCVVQPDPTAQELAEIAVATGNTARTIAGFEPKIAMLSFSTLGSGK
                     HEMVDKVVEATRLAKEMAPDMMIEGELQSDAAIVPEVGQKKAPGSKIAGNANVLVFPT
                     MQAGNIAYKLVQRIAGVEAVGPILQGMAAPINDLSRGCSVDDIVNLVAITANQAVGMK
                     K"
     gene            13285..14490
                     /locus_tag="PKN53_03555"
     CDS             13285..14490
                     /locus_tag="PKN53_03555"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOD88060.1"
                     /translation="MNVLVLNCGSSSIKYQLLDMGKEPVLKAKGLVERIGLPSGMIKH
                     KNNKDQKFELETPVSDHTVGINLVLDALLSKEHGVISSLDEINAVGHRVAHGGEYFTK
                     SVKLDEEAISKIEACIDLAPLHNPAHLLGIRAIQKILPKVPQTAVFDTSFHQTMPASS
                     YMYAVPYKMYENYKIRRYGFHGTSHKFVAQKACNILGWNFNEKKIITCHLGNGASITA
                     IKNGKSVDTSMGFTPVEGLMMGTRTGDLDLGALLFIMEKEKLSISQANSFINKQCGVN
                     GVSEISSDMRDLQAESEAGNERAKLTLDMYAHRVKKYIGAYAAIMNGVDLIIMTGGIG
                     ENSGHIRDLVFRDMDYLGISFDFDKNSKIFGKDEVLSKEGSKVKVMSITTDEELVIAM
                     DTVDLVLKK"
     gene            14518..15423
                     /locus_tag="PKN53_03560"
     CDS             14518..15423
                     /locus_tag="PKN53_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOD88061.1"
                     /translation="MNITKLDDIIEVVKSKSRKRLSVAFANDAHTIEAVSNAIDLGIV
                     EGILVGDTNRIEEVCKQENIDVKKFKIVHETDETKAANLAVKLINDKEADFLMKGLVS
                     TDKYMRAILNKETGLLPPKATLSHVVVMELERYPKLLVVSDVAVIPYPDLNQKIQIAN
                     YVINVAHDLGIEQPRLALIAATEQVSASMPSCVDAAIISKMAERGQIKGALVDGPLAL
                     DVAIDKECVEIKKLKSVVAGQADCLVFPNIEAANVFYKGSVKLAGAKLAAMLVGAKCP
                     AVLSSRGDSAEIKTYSIALAALSAK"
     gene            15449..16525
                     /gene="buk"
                     /locus_tag="PKN53_03565"
     CDS             15449..16525
                     /gene="buk"
                     /locus_tag="PKN53_03565"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOD88062.1"
                     /translation="MKYKILAINPGSTSTKIAIFENEKNIFLKNIKHSTDELKGFAKI
                     TDQYEFRKNTILNELKEAGLDINQIHCVMGRGGLNKPIPSGVYKVNEEMKNDMINSPF
                     GEHASNLGALIADDIAKNLDNVNAYIADPVVVDELEEIARITGHPNFERISIFHALNQ
                     KATARRYARETNQVYEKMNLIVAHLGGGISVGAHRKGKVIDVNNALNGDGPFSPERSG
                     TLPTAQLVSVCFSGKYTKEEIGLMIKGKGGLAAYLNTNDAYEVEMRAKEGDKKAQFIL
                     DAMAYQIAKEIGACAAVLKGQVDAILLTGGIAHNKQFCAFIDEMTSFIAPMKVYPGED
                     EMLALAMNGLMVLRGELEPKEYKF"
     gene            16538..16924
                     /locus_tag="PKN53_03570"
     CDS             16538..16924
                     /locus_tag="PKN53_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOD88063.1"
                     /translation="MKKIISTEKAPKAIGPYSQAVEVNGMLFISGQVPVNPETGKIPE
                     GITAQTEQVMKNIEAILKEAGYSFDNVIKSTCLLSDMANFKAMNEVYGKYYPNNPPAR
                     AAFAVKGLPLDAMIEIETIAAKSVKS"
     gene            17406..19205
                     /locus_tag="PKN53_03575"
     CDS             17406..19205
                     /locus_tag="PKN53_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD88064.1"
                     /translation="MIKLSYKFLFLLLFLHSNILLSQKYTANVPEIEYTELDDYYSVG
                     QEVQCTARVYSYGLVDDLCAIYYKVFKDDFTTPISSIEEYGHAEFAVRGQGENYVTQT
                     ISSGSGYISVKPMFTTYIAFTLGIFDNWCVNRNRPIVLSMNFTEPGLYKFKVEIQKCS
                     NSGSSILTSFQSNGNEYCSTAGTSHSDKATSSCTSPETLFTSEIIIPVCSESEITFLE
                     GETEYCSGDELFLTYRLGDNDNMIDQLSLPDWISVTFDEIAKTASLSGIIPDYDAENP
                     IIEFDVKSMNSSNPDICPSAVINQSITILPSESSIQNVAICQGGSYTFFDQEFSEAGT
                     HEVAVENSNGCYDKHYLNLTVNPIYNNSTHIQLCVGSSYEFAGATYTENTNIAHTFET
                     VYGCDSVVNLNLEFVEQFETNLKDTICEGEYYLFGDEEIFETGEYTLELSSAQSCDSI
                     VILNLIVNPTNEIILNETINEGETFVFNNQELTETGEYVSHLINEFGCDSTVTLNLTV
                     VTQVFTNEILYNQISVFPNPTNSILYINIGNVDENIKADIFISITDIAGKEIINKQRY
                     VGNLDLSTFKSGIYFVNVYLKNSKLTTKLIMKK"
     gene            complement(19213..19869)
                     /locus_tag="PKN53_03580"
     CDS             complement(19213..19869)
                     /locus_tag="PKN53_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88065.1"
                     /translation="MKLKKTLLLIIPLFLFSCVVQQDIVFNKDFSGSYKYSIDFKEYV
                     KYLKAEVDEKTDEENYLSNIDFEEYLNFVKSGLENILGIENLKIVNNAEEGEVYFSYD
                     FADINTLNQALRYSNLDTKTNEYSSYFEKKCKTITYVRPKIHTEENKNIEDENKIDFD
                     DNSLFTWVFNLEFATDVKKHNLANDTTISIQKGGRKFTETNNIYDYTNKETKWEFKLK
                     "
     gene            19979..21007
                     /gene="fbp"
                     /locus_tag="PKN53_03585"
     CDS             19979..21007
                     /gene="fbp"
                     /locus_tag="PKN53_03585"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HOD88066.1"
                     /translation="MASSRILTLNEVIIQKQSAFPYAKGELSRLLMHIGLAAKIVNNK
                     INKAGLETSIIGEADHINIQGEAQQKLDVYADEVFTKALQASGIVCGIASEECDEIVV
                     FDDEMSKEGNYVICMDPLDGSSNIDVNVSVGTIFSIYRRISPVGEKVTKEDFLQPGTK
                     QVAAGYIIYGSSTMLVYTTGLGTTGFTYDPSIGEFCMSHYFMVTPKNGKIYSINEGNY
                     VKFEPGVKKYLRWCQENDKETGRPLTSRYIGSLVADFHRNLLKGGIYLYPKTASAPNG
                     KLRLMYECNPIAFLAEQAGGKATDGYGNRILEIQPTDIHQRVPFFVGSKEMVEKAEEF
                     MREFKDEE"
     gene            20997..22139
                     /locus_tag="PKN53_03590"
     CDS             20997..22139
                     /locus_tag="PKN53_03590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD88067.1"
                     /translation="MKSKKIFIIYLFIIIPVFSFSQKIDPVLITTLDEVVSETSGLII
                     YNNKVWTHNDSGGDPKLYRIDSINGSVLEEKLILNATNIDWEDICHDGEYLYIGDFGN
                     NSSGTREDLKIYRILLADLDNEQLDSVASDIIYFTYDPSIYPEAIDKSNDTDFDCEAF
                     IAKDDSLYLFSKNWVNKKCYLYSLPKVPGHYMAQRLDTLDTQGLVCGADYNPETNTIA
                     LIGYVYGIPAPSFLVVLSDFDNNDFFGGNNLRYELSLNGYQTEAIIFRDNDRLWFSNE
                     NFLGHVQSLYEIKLSTLGLKEENLHSVCEIYPNPTNDKISIMVNLPGKIKYSISDAKG
                     NCMIKTSKKQFVNQVLDIDVSLLPAGIYFIELLGQNYKIQSKFIKL"
     gene            22236..24434
                     /locus_tag="PKN53_03595"
     CDS             22236..24434
                     /locus_tag="PKN53_03595"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOD88068.1"
                     /translation="MKKLIFLFISVWFSLVAFTTPDEGMWLLNKIAQLNYADMQKLGC
                     KLTAEEIYSINNSSLKDAIFQLQNSQGMGFCTGEIVSAKGLLFTNHHCGYEAITKLSS
                     PEHNYLDDGFWAKSYDEELSASDMRVSRVVRIEDVTARVLNGVTDETSEGEREKMVSK
                     AMKEIAEEAKKDTHYEASVSEMYQGGEYYLFVYEVFGDVRLVGTPPQSIGKFGGDTDN
                     WMWPRHTGDFSVFRVYMSPDGKPTKGYNKDNVPYEPLHYLPVSIAGVEEGDFAMIVGY
                     PGQTERYLSSYGMIYKRDFFNPAVVTILETQLKTLKVDMDADINVRLAKSDSYASSAN
                     GHKLFKGEALTLKTTDAIQQKEQLEKDFQKWVESDEARNAKYGKVLSSLKQGYEAFGP
                     ATLDMIYLSLGLLQSGDNVMNAQAFMNLANLLKDAKNNQTAIDATVENLKQMSEGMFD
                     KYFPNTDRKVFEASLKLYIADIPAERRNSVFNNYIFKNFKAKTEEESIEKFAQAVYTK
                     SIFTDKARMDAFLAKPSYKALNSDPIYSFVSNIFTDVFAIQMAYVGAMNDIGVNERLF
                     IEGLREFQANRTFYPDANSTLRLTYGTVNSYKPRDGVDYHYVTYSKGILEKEDPTDDE
                     FIVPAELKEKILKKDFGRYADKTGDLPICFLSNNDITGGNSGSPVINGKGELIGLAFD
                     GNWEWLCSNLLFSTELQQTINVDARYVVWVIDKLYDCKHIMSELDIRTTK"
     gene            24689..26107
                     /locus_tag="PKN53_03600"
     CDS             24689..26107
                     /locus_tag="PKN53_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOD88069.1"
                     /translation="MINLKKTIFILFIALSFGLRAGNVHIFGEAKEYASNTIIFYKYS
                     DRITFIKKQLFELEIDKDGKFDVNFEVDDITYVFAEFGIYHAYFYAEPDASYELILPP
                     FQEKSLSDIFNPFFSSETIQIGIKNMQPTDLNYLILDFDYYYFRYLDLKYIELYVNGL
                     QTDVDTFITEINSKFNNYDNQYFQDYKKYRIAALKNIATQKQYEDQIVYSYYTKAPVL
                     YDNPAYMDLFNNIYAKYFDLLLVAPHGDSLYAIITYGHSISRLKKFLSRNLSLRNNQF
                     QELVILKGLNDAFGGGNLQWLPLLLTLDSVHLSTQYPFHQDVAQNIADNVLSMAKGTI
                     APPFELPDTAGEMHSLKEFKNKYTYIQFANTKTYSCQLEFAMMGNLHKRYKEVCNFVT
                     ILTDSDIESAKAYINKNEFGWTFLFTEINSQTISTYKIDTYPTYFLVDTRGTLLMSPA
                     PSPSENFEKYFFKYVEDKLPKK"
     gene            complement(26112..28901)
                     /gene="polA"
                     /locus_tag="PKN53_03605"
     CDS             complement(26112..28901)
                     /gene="polA"
                     /locus_tag="PKN53_03605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018362353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOD88070.1"
                     /translation="MKNKIFLLDAYALIFRSYYAFIKNPRINSKGLNTSAIFGFVNTL
                     EEVLNKEKPSHIAVVFDPSGPNFRHEIFPEYKANREATPEDIKLSVPYIKQILEAYKI
                     PVIQVSGYEADDVIGTLAKKFGNAENDVFMMTPDKDYAQLIDENIFMFKPARGGKAAE
                     VIGLTELNQQYGVSNPEQVIDILALWGDTSDNVPGVPGIGEKTASKLIDKFGNIDSIY
                     ANIHKIVGKQKESLEKNKEQLYLSRHLVTIDTKVPVETNMEELKLEEPDFVKLNQIFD
                     ELEFRTLKNRVIGENIKQVKQKTVPNQGNLQLDLFSSVSNSETAQTEIPDITTENRDV
                     DYKLIETLYEIEELIPKLNEHKEICLDTETTSLDIMQAEIVGISLSVVENQAYFIILP
                     QGFDTAKEILEMFNPIFQNKEKVIIGQNLKFDLAILDKYGINVKAQLFDTMIAHYLIE
                     PEQKHGIDFLAATYLNYTMISYEDLVGKGKNEKHIRDVDTKLLSFYACEDADITLKLK
                     NIFTPILKENNLEPLFFEVEMPLVKVLANMEKTGVSLDVEIVKEQSQILRKELLQIEE
                     KIYELAGETFNIASPKQLGIILFEKLKIISNPKKTKTKQYSTGEEELLKLKDLHPIIN
                     EILEFRSINKLINTYVDVLPQLVNPKTNKIHTSFNQTVTSTGRLSSNNPNLQNIPIRD
                     ERGRELRKAFVASDSEHVFLSADYSQIELRIMAHVSEDKNMLDAFNKFSTDIHSATAA
                     QIFKVDAENVTREMRNQAKTANFGIIYGISAFGLAQRLNISRTDAKTLIDNYFETFPG
                     VKAYMEASIAKARENQYVETIFGRRRKLADINSNNAIVRSYAERNAINAPIQGSAADI
                     IKLAMVNICKKFEENNLKSKMILQVHDELNFDVYKPELEQVKQIVKDEMQNVVTLKVP
                     LIVEIGVADNWLEAH"
     gene            complement(29034..29570)
                     /gene="rimM"
                     /locus_tag="PKN53_03610"
     CDS             complement(29034..29570)
                     /gene="rimM"
                     /locus_tag="PKN53_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOD88071.1"
                     /translation="MFFGKEELIKIAEIQRTHGVDGKVLLNISQDFDKSIFTEDLPVF
                     LIIDGMPVPFFIEHSKSQGKNVVVKLRHIESLEKAEKFVGTEIMTLKSLFDDFDEDED
                     NEKDIEGYSVYDKKFGFIGQVCMLNNIPGNPVIEINFKEKIIIIPYAEDIILEINDEK
                     KEIQISAPEGLIDLYLEM"
     gene            complement(29573..30202)
                     /locus_tag="PKN53_03615"
     CDS             complement(29573..30202)
                     /locus_tag="PKN53_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOD88072.1"
                     /translation="MAVKLRLARHGRKRQPYYYIVAADSRAPRDGKFIERIGSYSPVS
                     NPAKIELDFDRALTWLMNGAQPSDTVRRILSYKGVLMKKHLLEGVKKGAFNEEEAEKR
                     FKAWLAEKEAKISSKVSNLELAQREADKARLEAERKVKEQKAQELAKKFAAEAEAAKA
                     AQEAEKAEEATEEAKAEAPEAKAEEKVEAKVEEKAEEEKAEETTENTQE"
     gene            30388..31899
                     /locus_tag="PKN53_03620"
     CDS             30388..31899
                     /locus_tag="PKN53_03620"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOD88073.1"
                     /translation="MFYKKIIKIAIFLTILAGTTSCYREEIVLIPETDDNLQLESLLY
                     FNSKPCFFDEATNTLTYSIVETELQNFNPKLSFSPLVKVYYKGKLLKNSEYLQSDSEN
                     FEYKKAYSFYFVTKNDSVEIQLRFTPLPIVRLSKTLTIFDEPKIPAYFQIVYPESFKS
                     DYSCYIGVEIRGATSQIYPKKSLGFNTLANQDIKSYQMTTLVGNRENWKWILDAAYMD
                     NLRVRNSVSFYVWAKISNSPNHFSINTTPVELFINNEHQGLYFLGENFSNEILNLKAN
                     SEVLYKAYDWSEGATRFEFANDNISKNEFWDGWEQKHPDPKFYLNWNPLKDLRNFIVN
                     SSDENFRDSISYYIDIDNFIDYYLFMNLLFALDNAGKNTFLIKSAQTGKFVIIPWDLD
                     ATWGLFWDGTYNEPTFITTNYLYDRLFELNPNDFKTKLKNRWFALRQNVFSQEEILLE
                     FEKPLSLILKSGIIEKENLIWKLNINTEAEYNYIKTWISARLNYLDNYFSNLS"
     gene            32018..>32464
                     /locus_tag="PKN53_03625"
     CDS             32018..>32464
                     /locus_tag="PKN53_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88074.1"
                     /translation="MKRFVFLLTIAIITITHVKAQNYKVGVSLENIVSDFNWNKPLED
                     FMVTIQPFSLETKMPIKLGVCVQRNFKVISPSVHVNVIYRNIKYAKHYTSITNFNHYS
                     LNVPINLNYRKRVSDESTFVANIGGGINYILTSNDKTNSGMYNDGDL"
BASE COUNT        11724 a         4343 c         5370 g        11027 t
ORIGIN      
        1 tttaccacaa attaatacaa gttatcataa acaaccttgt gaaacttgac actttacaat
       61 taattcatga aaagaataat tttaacaata ttgacaataa gttttgcttt gtttgcaaaa
      121 aatgcagtat attctcaaga tatacatttt agtcaatttt tctccgcacc tcttttctta
      181 aacccttcta acactggtga ttttgaagga gattatagag ccgctttaaa taacaaaaac
      241 caatggaata catttacaaa tgcgtattca agttttgcag gttctataga tgccggcttt
      301 gatgatgttt ttatctctca atcacgttcg ggaattggtt tacaaataaa taacgatatt
      361 gcaggagacg gtaggttcgg aacaacacaa ttatatctta atcttgctta tcaattaaat
      421 gtggataaaa ctcaaaaatt tagactagga ttgggattgg ctggcggtta tgtaatgcat
      481 ggtattaatt taaataattt atattttgga aatcaatatg atggagaaag atttaatgaa
      541 aatttaccat ctgatgaagt tttaataaat caaaaaatgt catatccaga ttttggaaca
      601 ggagcaaaat taaaatataa atataatcat aaattaaatt ttgaattggg attttcagca
      661 tatcatttgt tgcaaccaaa acgtagcttt atagaaaatt cagaaatgtt tttgccaata
      721 aaatattcta caattctttt tgcagaatat caattaaaag aagatttatt tttggaggct
      781 catgctttgc atatggttca gcaaaaacat aatgaagtag atgtaggtgg aatttttcgt
      841 ttagattata atccacttga tttgcaatca atatattttg gtggattggt tagggcaaaa
      901 gatgctggaa ttattgtgtt tggctttaaa tatcataatt ttaaaatatg ctttaattat
      961 gatataaatt tctcaaaact aagcacaatt agtagaggaa aaggaggagc agagttttcg
     1021 ataatttatg tattctttaa accacgacca tttcaagctc cagattataa aaaatgtccg
     1081 gattttattt gatgaaaaga tttttaattt acatagcagt aatatttata ggaataaact
     1141 ctgtagcaca aaccaaagaa gtggttaatc ttgtagtttc tggggacaaa gcttttaaag
     1201 aaaaaaattg gtatggagca gcaaaactat acgaacaagc actcaaatca aatcaaagaa
     1261 tgcgcgatat tgtatggaaa gctgccgaag cttatcgctt ggataatgac tatgtgagag
     1321 ctgctaaaca ttatagattt ttagcagaaa aaaatcctga aaaatatcct gacgcaatat
     1381 tttattatgc ggaaatgtta aaagcaaacg aagaatttat taaagcacag tataattatc
     1441 ggcaattcat agcaattaaa gaaaatgacg aaactaattt gaaagtaaaa aaatcaatgc
     1501 aagaaatatt gaattgcgaa aaagcttgga aaatgtacaa caatccatct ggaatagaaa
     1561 tttttcaatg cgacacaaat ataaattctg tttacagcga cttttctact gcattttata
     1621 aagattctat tttaactttt gcttcaatac agcctgttgg cgacagtatg aaagattata
     1681 agtcaaaaat ctatcaaata aatttttttg agaataattc tgaggctgaa ctttttgacg
     1741 aaacaataaa taaagttgat tttgacattg caaatccgca tttttcaaaa gatgggaaaa
     1801 aaatgtggtt tacagtttcg gaatcttatc aaactggaaa tacttatatt tactcctctg
     1861 aatatgttga taatgaatgg acagaaccac aaaaattgcc agatgttatt aatagacctg
     1921 gatacaactt cacacaccca tttatgttag aaagagaaaa tgaaaataat atattgcttt
     1981 tttcttctga ccagccggga ggggagggag gctacgatat ttattatgta gaagtgttac
     2041 cagatgactc gtggggagta gtaagaaact taggaagacc aattgtcgaa gataaaactt
     2101 tacaaaattt tctagacact agctctataa taaatactcc aggaaatgaa attacacctt
     2161 tttataatat taaagattca tgtttatatt tttcgtctga ttggtatgaa aatttaggag
     2221 gttacgatat ttttaaggta aaaggagact ttacaaaatg ggaaaaagtt gaaagtttag
     2281 agtatcctgt taatactgct caaaatgata cttattataa aatatatccc aaacaaggat
     2341 tagctttttt taccagtaat agaaaatctg catttgcagt agaaaagcaa agttgttgca
     2401 acgatttgtt ttattacgaa ctagaaaaag aattaccgcc agaattaata actgagaaaa
     2461 aaataacaac aattattact aaaacaaaac ttttagttcc aattacgctg tattttcata
     2521 acgaccagcc agttccaaac tcgtgggata ctgtaacaag tataaattat tcaacaagct
     2581 attttgatta tttagccttg caagaaactt atagaaaaat gtattcaaaa ggattgggaa
     2641 aaacagaaaa gctaatggct attgatagca tagattatta cttttcttat aatgttcaag
     2701 ataattacag taagctattg gaatttacaa gtttaatgca cgaacttttg attgaaggtc
     2761 aaaaaattat tgttacaata aaaggctata caagtccact taatactgca gcctataata
     2821 ttaatcttgc taaacgccga atatcaagtt tagttaatta tttcgccgaa tacgacaatg
     2881 gcatatttct tccttacata gaatctggac aattagagta cgaatttgta gcttttggaa
     2941 aaacactgtc agatggcaaa gttagcgatg acccaaaaga cccgcgaaat tcaatatata
     3001 gtccagcagc atcgagagaa agaaaaatag aaattattgc aatttctgtc gaaaatctag
     3061 aacaaaatta aatttttatt gttagtaatt atgtaatttt gtagcggaaa gagaaactga
     3121 aattttatta tgacaaagtt tttggaaatt attgcaaact taaactggca tggtatatta
     3181 caagaaacat taatgattac aagttttgtt gtaattgtta tgttgttgat tgaatttctt
     3241 aatgttttaa gtcaaggaaa attaacaaaa gttattagca aaaaaccagg aatgcaaatt
     3301 ataacaggaa cggttttagg acttatacca ggttgcatgg gtgtttttac ggcagtctct
     3361 ctttatactc accgactttt tacttttgga gcaatgcttg ctgctatgat tgcaactgtt
     3421 ggtgatgagg cttattttat gttagcacta atgccaaaaa cagcaatatt tatgtttgct
     3481 attttagctg tattggcaat agttttaggt tttgttgctg ataaatactt aaaattaaaa
     3541 aattatgcag ctgacaaaga attttcattc gatattcacg accacgatga ttgttgcgat
     3601 aagccaagta gtactttttc atttaagaac tttagttttt tgcccaagcg aatgataata
     3661 ttagcaataa tcctagttat actaggctta acctttggtg gagttattgg acatacacat
     3721 tcagataaat taattttttc actccctgca gcaaattccg taaaaaaagt ggaaatgagc
     3781 gatttgaata ttacacatat tcataatcat gattgcgaac acgagcatga acatatttct
     3841 gaaacctcgc ataatgaaaa tttgcatcat aatcatgaac acggcataga ttgggttaga
     3901 gttacgattt taatatctgc attttttgca ctgattgtag tactttttac taaagaccat
     3961 tttataaaaa aacacttgtg gcaacatcta ataaaaaaac atttgcctaa aattttaatt
     4021 tggatatttg taactctgct agctgtaaat attttattac aatttgcaaa ttttggaaac
     4081 tggatttatg accacctctt tattgttttg ctaatagcaa tagcagtagg aataattcca
     4141 caatcaggac ctcatttagt gtttgtagtt ttattcttgc aaggaacaat acctttcagt
     4201 attttgttgg caaatagtat tgtgcaaaac ggacatggtt cactaccact gttagcagaa
     4261 tcaaaaaaga gttttgtaat tatgaaaact ataagtataa ccattggact aattgtagga
     4321 atatctggat tgttgtttaa tttttaataa ttgactaaaa aaacaaaaaa aaatcgaata
     4381 aacgctaatt tatacaaact ataaatattt cttacacacg cactaatagc actaaaatgt
     4441 aaaaatttac ttatttagaa taaggctgtt ttttgttaaa ttcttgatga aaaacattta
     4501 aataatttgg attatttgaa aataattttg tattttcgca ttgtaatact taaatacaat
     4561 tattaaaaaa ttaaaataat aaaacaatga gcacacaaga atttaacaca agactattag
     4621 acttagaaaa gagcttgaga tatttcgctc ttagtctaac caaagatatg gagacagcaa
     4681 aagatttagt tcaagatact tttgtaaaag ctattcaata tcgcgataaa tatacagccg
     4741 ataataatat taaggcttgg ttatttacaa ttttacgtaa tacatatttg aatcaagtaa
     4801 ctaaattgtc tgcaaaaaac accattaatg atgagacaga agatgagtat attttaaaaa
     4861 atactatgta cgaagagtat aatgctgaaa acagtattaa tactaaagac attcaatcaa
     4921 ctatagataa tttagaagat gattatagaa taccttttca gttgtttgtt gatggatata
     4981 agtacaagga aattgccgac aaaacaaatc tacctatagg tacagtaaaa agtagaatat
     5041 tttttgctcg acgtcagtta atggaaaatt tagaaagttt taaataattt ttacaaacta
     5101 caacttagtt gagtgccttt aagtaaaatt aaggcacaaa taattgagct gtcttagaag
     5161 tgttcaaaag tgtttgagca tttataagac agtttttttt gttttttaca ttttaaattt
     5221 gtttaaaatg taaaaatttt gttattttac gttttcaaaa attcatacat gagtaaatat
     5281 tttcttttgt taataagctt aattttgcca ctaacatttt ttgggcaaaa gcatttgaga
     5341 aatgcaaatg tcgctttaga caaagaacaa tatgcacaag caattcagct atatttaact
     5401 caaattgcaa aatctaaaaa caaaccacgc attattgcag aatcatcatt taaaataggc
     5461 tattgctaca aaaaactatc tcagccagaa gaatctgcat tatactttaa aaaagccatc
     5521 gaccttggat attccgaccc tgtgcaatat ttatattacg gtgaagcttt acaaatgttg
     5581 caaaaatatg attcagcctt aattcagtat gaaattttta aaaagttagc accagagcat
     5641 aagttagcaa acaaaggaat agaaagtata gattttactt tttcttcaat cgaaaatcca
     5701 actcgccata aagtagagtt atttgcgatt ttaaattctg gcgaacatga ttattgtccc
     5761 ttttatgaag ttaggaacaa acacaaaatt tattttactt cgggacgtta tgctccaact
     5821 catgtttcta taagtcctga atctggtgaa tattgttcaa atttattttt ttcagaacaa
     5881 gataaatttg gaaaatggtc aaaacctgtt attgtaccag gtttagtaaa tactgttgat
     5941 gaggagggag ctgctttttt aaatcgaaaa tcaaataatt tgtattttac aagatgccaa
     6001 tacgacaaaa aaaaggataa aggttgtaga atttttgtgg caaagcgtgt gggaagttat
     6061 tggggacaaa tcaaagaagt agaaattctt ggaataccag aacatatttc aataggacat
     6121 cctgcaatta gcgatgatga attaacttta tattttgtag cagactcttt acttggaggc
     6181 ttaggaggaa aagatattta taaagttgtt agagaaagaa aaaatgcacc ttttggttac
     6241 cctcaaaatt taggacacta tataaatact gaagctgatg aaattcatcc ttatattaga
     6301 agtaatggag atttatattt ttcgtctgat ggacatccag gaatgggtgg ttttgacatc
     6361 tttagagcta aacatatttc agggattaag tatgaaattt ctaacttagg atttccaata
     6421 aattcatcta gagacgattt tggcatagtt tttataggta tgaaagaaga gggattctta
     6481 tcttctacaa gaactggagg tttgggaaaa tcagatttat attattttct gcttccttct
     6541 ttagaattta cgctctcggg aacaatttat gataaaataa caaaagaaaa aatggcaaat
     6601 gtggatatac aagtaatgaa tgatgaatat attttgatgg attcgcaaca atctgacact
     6661 aacggaagct atgaaattga cttattgcct gaaaatgatt atataattta ttataaatta
     6721 aacggctatc gtgttgctaa agagatagtt aatacaaaaa cattaacaga agcaaaacat
     6781 tttttaagag atgtgtatat agaaaaataa agtttattat atttgaagag tttttaaatg
     6841 aaaaaaccta agactatgaa acatattgtt ttttttattt tagtaatgat tataccggtg
     6901 ttattaacag cacaaagttc tttgattacc aaaggagatg agtattataa cacatatcag
     6961 tatgaattag caaaagacta ttatttgaag gctattccga gtttgagaac tcaagaagat
     7021 aaagcctctg ttaattacaa acttggtatt tgttttaaaa atttaggtga ctctgataaa
     7081 tcagaattgt attttcaagt tgctaaaaat aattatgtaa aaggagttat taaacctgac
     7141 gttttgctgt tttatgcaga tgcattaaga atgaacggta aatatgaaga tgcaattgat
     7201 gtttataaac aatatcttca agtttcgcct caagaccata gagcaattgc tggaatggaa
     7261 tcttgttcta tcgttccaaa gtggatcagt cgcccaacaa gatataaagt taccaatgtt
     7321 tcaagtttta actcccaaag ttttgacttt tctccagcat ggggaagcaa ggattacaga
     7381 acattgtatt ttacaacctc tagagaagga acgataggag caagaagcaa ttataaaact
     7441 ggtcaaaaat ttacagatat attttcagta actcaagata gaaaagggtc atggagcgaa
     7501 ccaacacccg tagcaggagg aattaattca gaagatgacg aaggtgcatg cacagtatca
     7561 aacaaaggta cagaaatgta ttttactaga tgtaaagcag gcaaaaaagt ggacgagcct
     7621 tgtaaaatat attatgctag taaaaaagga aatgtctggg gagaagcaca gttagtaaat
     7681 attccaggct tcgaaaatta tgaagttgga cacccagcat tatctacaga tgataaatat
     7741 ttatattttt cgggagaagc accaacagga tatggcggaa tggatattta tagatctaca
     7801 ataaaagcta acaatgcagg acatccaata aatctaggac caatagtaaa tagtcttgga
     7861 gacgaagtgt tcccaacagt aagggcagac ggaactttat attttgcgtc agatggacat
     7921 aaaggaatgg gggggtatga tatttataaa gttgttaaag atgacaaagg aaatattact
     7981 gatatagaaa atttaaaata tcctattaac tcttcatttg atgattttgg aattattttt
     8041 tatggaagtg aagaaactgg atatttgtcc tcaaatagaa aaggaggtaa aggaggagat
     8101 gatatttatt cttttgtttt accaccactg tctataactt taaatggtgt agttagagat
     8161 actacagata tgttaaaaat aagaacgcta aaagatgtga aacttagctt atttaatgat
     8221 gatggacttg tggctgactt cctttcttca gaaaatggaa cttttaatta taagttagat
     8281 gtaaataaaa actataaaat caaagcagat ggtggcaaag attattttgc aacatcagtt
     8341 caatttacaa caatgaaaat tgaatatgat acggtaataa atgtagttat aaaccttgct
     8401 agaataccac gtgtaattgt attacctaat attttatatg aatatgataa agctacattg
     8461 ttacctgaat caactgtatc tttagacggt ttggtaaaaa ctttagaaga taaccctcat
     8521 attgcgattg aacttagatc tcacaccgac tttagaggag cagcagatta taacatggat
     8581 ttatctttga gaagagcaaa atcttgtgtt gattatctaa tttctaaggg aataaaaact
     8641 gatcgtttaa cagctaaagg ttttggtaaa agcgaaccta gagaaataac aaaagaatta
     8701 gcaaaagaat ttccatactt caaagaggga gatattttat ctgaagatta tatcaataga
     8761 cttaaagata ataaacaaaa agaagatgct catcaattaa atagaagaac tgagtttagt
     8821 gttatttcaa cagagtacgg aaaagataaa gaacaagaaa aagcaaaaga tgaaattcct
     8881 caagggcaac caggctcttc aattataaaa gatactggaa gagaattcta aatttataaa
     8941 aatatacaaa taaaaagggc tgtctttttt gaaacagccc ttttttgttt tatcaagtaa
     9001 actttttata ccatgaaaat tattacatta aaatcgaata ttatatcttg tcagaattcc
     9061 ccctttggtt aacaataagt taattgttgt aaatttaatt aagaggataa tttgctgttg
     9121 cctgctgaga ggtagcatta aggatttgag agtgtatcat taacctcagg gagaataata
     9181 tttcaaagaa attctgacaa ttaagtttgt aaattattaa tcattaaact tatacaaaga
     9241 tacaaaatat atttatataa ataaaatttt ttgttattaa aattaaaaaa ataaaataat
     9301 agtattaata tttgtttgtt tgttaattat caatgactta gatggagttc taaaattgtt
     9361 tgttttattt taaaagaaat agttgttatt tcaacttata ttctacttgg gtattagggt
     9421 ctgtgctagg tttaaaaaat tcaataggat tgtcaaaata atcttcgggt tgtttaaatt
     9481 tattttgatt ttctttagcc cattcttctt ttgctctcca ttcttccatt aattcgtccc
     9541 aattatcagg cacgcctttg tcgtaattta caatttcgcc ttttggatta tccctataaa
     9601 ttttccattc accgtctttt ttaccattta catacatgcc ttcttcatca atcataccac
     9661 tatttgaata aaatttccaa tagccgtctc gagtgccatt tttatatgta ccttcttcaa
     9721 ttttagctcc agattgaaaa tattttgtgt attgaccgtc aagtttacca gccttatagt
     9781 tacaatttaa agctaatttt tcgatgtttt gataaaatat taaaacattg ccttctgcta
     9841 ctccatgatt gaagtttata actgcagact ttttaccgtc ttcaaaatac ataatccaag
     9901 ttagatgttt attattatca ctgtcaaaat aacctttagc tgcataattt ccattatccc
     9961 aaaatagttc agctttagaa ccgccactct catcataatt taaaacagat ttgattttac
    10021 cattttgata atagtaagta aaaacgcctg taggaagatt gtcaataaaa aatcccctat
    10081 atcttacttg accattagga tattttttct cccattttcc ttgcttgtta ttattaatat
    10141 ctacaaaatt aattaaacta tcattttgtc tattgttctg agcaaatgaa atagaaccga
    10201 aaaataaaac tattagacct ataataaata tcttgttcat cataaattgt tttttactat
    10261 aactgtaatt ttaattaaaa tattgttatt gttttttatt gcaaaactca taccttattt
    10321 aaaagcaaat ataaacaaag attttatttt gtaaaaaata tgttgtaaat attgttttat
    10381 ttttgaataa aattttttaa atttgctacc agtttaaaaa taaattaaaa aaataaatat
    10441 tatataaaat acataaaaaa tgaaaaccac tgcatttaca aattatcaca ttgcaaaagg
    10501 agccaaaatg gttgaatttg cagggcataa catgccaatt gaatttacag gaataaatga
    10561 cgaacacgtt acttgtcgtg aaaaacttgg agtttttgac gtgtctcaca tgggagagtt
    10621 ttgggtaaaa ggaccaaaag ctgtagattt tttacaaaaa gttacgtcta acaatatttt
    10681 agatttgaca gatggtaaaa ttcaatattc ttgctttcct aatgataaag gtggaatagt
    10741 tgatgacttt ttagtttatc gttttaatgc agaatcttat ctgttagttg taaatgctgc
    10801 aaatattgat aaagattggg cttggtgtac agcaattgct agtaaaatgg gaatgaaaat
    10861 tggcgaagat cttatcaatg cttcagatga atattgccaa ttagcagttc aaggacctta
    10921 tgctataaag gctatgcaaa aacttacaag tgcaaatatt acagatatgg aatattatac
    10981 ttttaaagaa attccttttg caggaattga aagtgtaatt ttttctataa ctggatatac
    11041 aggtgctgga ggttgtgaaa tatatgcaaa aaataaagat gcagaaaaac tttataatgc
    11101 tgtttttgaa gcaggagaag aatttggaat aaaatctata ggtcttggtg ctagagatac
    11161 cttgcgttta gaaatgggat tttgcttgta tggaaatgat attaacgaaa ctacttcacc
    11221 aatagaagca ggattagggt ggattacaaa atttgttgac ggtaaagact tcgtaaatag
    11281 agaatatcac gaaaaactta aagccgaagg tgcaagcaaa atgttaaaac ctttcgaaat
    11341 gattgataga ggaattccac gtcagcatta tttagtgtat gatgcagaag gaaatgaaat
    11401 aggtgaagtt acatcaggaa ctatgtcacc aagcatgaaa gttggaatag gaatggctta
    11461 tctaaataaa gggttttgga aagttgatac cgaaattttt atcggagtta gagataaaag
    11521 attaaaagct aaagtggtaa aagctcctat ttttaaaaat caaattcaag tagtaaaata
    11581 aaatttcgag acaagcacat agcttgtctc tttttattat aaaatgaaaa tcatctgtat
    11641 aggacataat tacaaagatc atatcgagga aatgaatgct gtgttgcccg aaaaacctgt
    11701 attcttcctt aaaccagatt caagtatttt acaaacagga aaagattttt atttgccaga
    11761 tttttcaaac gatattcaat acgaattaga aataatcgta aaaattcata gattaggaaa
    11821 acatattgat aaaaaatttg cacatcgcta ttatagtcat ataggtgttg gaatcgatgt
    11881 taccgctcgc gatttacaaa gagattgtaa agaaaaagga ttgccttggg aaattgcaaa
    11941 agcttttgat ggctcggcaa tgctctcgga atggatacca atagaagaaa ttgaagatgt
    12001 aaataatata aacttcaaac tcatgcgtaa tggcgagcta gttcaattgg gaaatactaa
    12061 aaatatggtt tttaatattg atgaaataat agaatatgtt tctaaatttt taactcttaa
    12121 aattggagat ataatcttta ctggaacacc aaaaggagtt ggtaaacttg aaattggaga
    12181 tgaattaaaa gcctttatag aagaaaaaga attattaaat attaaaatta aataaaaata
    12241 ttaaaaatgg atttgttaca aaaaattaaa gaaaacgcac gtaaacacga aatgcgaatt
    12301 gttctaccag aaggaacaga aataagaact ttaaaagcag ctaatcagat tattgaagaa
    12361 aaattagcaa aaataatttt attaggttcg cctgaagtta taaataaaac tgcttcagaa
    12421 aacaatttga caaatattaa aaatgcaata attattgatc caaaaaatca tgataaaaaa
    12481 gaagcttata ccgatttatt agtagaactt cgtaaatcaa aaggtttaac taaagaagaa
    12541 gcttcaaaat tagttgagaa cccattatat ttagcaactt taatgataaa agcaggtgat
    12601 gttgatggtg aagttgctgg tgcagataat gctacaggtg atgtgcttcg tcctgctttt
    12661 caagttgtaa aaactttacc aggcatttca gttgtttctg gtgcttttat aatgatttta
    12721 aatgacaaaa aatttggcga agatggttta atggtatttg cagactgtgt agtgcaacca
    12781 gatccaacag cacaagaatt agccgaaatt gctgtggcaa ccggtaatac tgcaagaaca
    12841 attgctggtt ttgaaccaaa aattgcaatg ttaagttttt caactttagg ttcgggtaaa
    12901 cacgaaatgg ttgataaagt tgttgaagca acaagactag caaaagaaat ggctccagat
    12961 atgatgattg aaggtgaatt gcaaagcgac gctgcaatag taccagaagt tggacagaaa
    13021 aaagcaccag gaagtaaaat agcaggaaat gcaaacgtgc ttgtattccc aactatgcaa
    13081 gctggaaata ttgcatataa attagttcaa agaattgctg gagtagaagc agtaggacct
    13141 attttacaag gaatggctgc tccaatcaac gatttgtcaa gaggttgttc tgttgacgat
    13201 atagttaact tagttgctat tacagcaaac caagcagtgg gaatgaaaaa ataattatat
    13261 aaacttaatc taaaaaataa taaaatgaat gtattagttc taaattgcgg aagttcatca
    13321 atcaaatatc aacttcttga tatgggtaaa gaaccagtat tgaaagctaa aggtcttgta
    13381 gaaagaattg gtttgccaag tggtatgatc aagcataaaa ataataaaga tcaaaagttt
    13441 gaattagaaa ctccagtttc agaccataca gtaggtatta atctagtttt agatgctttg
    13501 ttgagcaaag aacatggagt tatttcatct ttagacgaaa ttaatgctgt aggacatcgt
    13561 gtagctcatg gtggcgaata ttttacaaaa agtgtgaaat tagatgaaga agcaattagt
    13621 aaaattgaag cttgtataga tttagcacca ttacataatc ctgcacactt attaggaatt
    13681 agagctattc aaaaaatatt accaaaagtt ccacaaacag cagtttttga tacttctttt
    13741 catcaaacaa tgccagcaag ttcatatatg tatgcagtac catataaaat gtatgaaaat
    13801 tacaaaataa gacgttatgg tttccatgga acaagtcata aatttgttgc tcaaaaagct
    13861 tgtaatatat tgggttggaa ttttaatgaa aagaaaatca taacttgcca cttgggtaat
    13921 ggagcttcaa ttactgcaat taaaaatgga aaatcagttg atacttcaat gggatttaca
    13981 ccagtagaag ggctaatgat gggcactaga acaggagacc ttgatttagg tgcattattg
    14041 tttattatgg aaaaagaaaa acttagcatt tctcaagcaa attcatttat aaataaacaa
    14101 tgcggagtaa atggagtttc tgaaatatca tcagacatga gagatttaca agcagaaagt
    14161 gaagcaggta acgaaagagc aaaactaact ttagatatgt atgcacatag agttaagaaa
    14221 tatatcggag cttatgctgc aataatgaat ggagtagatc taataattat gacaggagga
    14281 attggcgaaa attctggaca tatacgtgat cttgttttta gagatatgga ctatttaggt
    14341 attagttttg attttgataa aaacagtaaa atattcggta aagacgaagt gttaagcaaa
    14401 gaaggttcta aagttaaagt tatgagtata actacagacg aagaattagt gattgcgatg
    14461 gatactgtag atttagtgtt aaaaaaataa ttaatcataa caaaaaacaa acgagtaatg
    14521 aatataacaa aattagatga tattattgaa gttgtaaagt caaaatctcg caaaagatta
    14581 tcggtagctt ttgctaatga tgctcacact atagaagccg ttagtaatgc tattgattta
    14641 ggtatagtag aaggaatttt ggtgggagat acaaatcgta tagaagaagt atgcaaacag
    14701 gagaatatag atgtaaaaaa gtttaaaata gttcatgaaa cagacgaaac caaagctgca
    14761 aatctagctg taaagctaat aaacgacaaa gaagcagact ttttaatgaa aggtttggtt
    14821 agtacagaca aatatatgag ggctattttg aataaagaaa ctggactttt acccccaaag
    14881 gcaactttgt cacatgttgt ggttatggag ttggaaagat atccaaaact tttggttgtt
    14941 agtgatgtag cagtaattcc ttatccagat ttgaatcaga aaattcaaat tgcaaactat
    15001 gtaattaatg ttgctcatga tttaggtatt gaacaaccaa gacttgcttt aattgcagct
    15061 actgaacaag tatcggctag tatgccatct tgtgttgatg cagcaataat ttcaaaaatg
    15121 gcagagcgtg gacaaataaa aggagcttta gtcgatggac ctttagcttt agatgtagct
    15181 attgataaag aatgtgttga aattaaaaaa ttaaaatctg tagttgctgg acaagcagat
    15241 tgtttagttt ttccaaatat agaagcagca aatgtttttt ataaaggttc agtaaaatta
    15301 gcaggtgcaa aattagcagc aatgcttgtt ggagcaaaat gtcctgctgt tttatcatca
    15361 cgtggcgatt ctgccgaaat aaaaacttat tcaattgctt tagcagcatt atcagcaaaa
    15421 taatataaat taaaataggt ataaaattat gaaatataaa attttagcaa taaatccagg
    15481 ttctacttca accaaaattg caatttttga aaatgaaaag aatatatttt taaaaaatat
    15541 aaaacattca acagacgaat taaaaggttt tgccaagatt actgatcaat atgagtttag
    15601 aaaaaatact attttaaacg aacttaaaga agcaggactt gacataaatc aaattcactg
    15661 tgttatgggt agaggtggac taaataagcc tattccttca ggtgtttata aagtaaatga
    15721 agaaatgaaa aacgacatga taaattcgcc ttttggagag catgcaagta atttaggagc
    15781 cttaatagca gatgatattg caaaaaactt ggataatgtt aatgcttata ttgcagatcc
    15841 tgttgttgtt gacgaattgg aagaaattgc tcgcatcaca ggacatccaa attttgagag
    15901 aatttctatt tttcatgctt taaaccaaaa agcaactgct agaagatatg ctcgtgagac
    15961 aaatcaagtt tatgaaaaaa tgaacttaat tgttgcacat cttggtggag gtatttcagt
    16021 tggtgcacac agaaaaggaa aagtaattga tgttaacaat gctttgaatg gagatggacc
    16081 tttttcgcct gagcgttcag gaactttgcc aacagcacaa ttagtatctg tgtgttttag
    16141 tggaaaatat acaaaagaag aaattggttt gatgataaaa ggtaaaggag gtttagcagc
    16201 ttatttgaac acaaacgatg cttacgaagt tgaaatgaga gcaaaagaag gcgataaaaa
    16261 agctcagttt atattagatg caatggctta tcagatagct aaagaaatag gagcatgtgc
    16321 agcagtactt aaaggacaag tagatgcaat tcttttaaca ggaggaatag cacacaataa
    16381 acagttttgt gcttttatcg atgaaatgac aagttttatt gcacctatga aagtttatcc
    16441 tggtgaagat gaaatgttgg ctcttgcaat gaacggactt atggttttaa gaggtgagtt
    16501 agaaccaaaa gaatataaat tttaatgttt aaataaaatg aaaaaaataa ttagtacaga
    16561 aaaagcacca aaagcaatag gaccatacag ccaagctgta gaagtgaatg gaatgctttt
    16621 tattagcgga caagtgccag taaatcctga aacaggaaaa attccagaag gaattacagc
    16681 acagaccgaa caagtaatga aaaatattga agctatccta aaagaagcag gatacagttt
    16741 tgataatgta attaaatcaa cttgcctgct tagcgatatg gcaaacttta aagcgatgaa
    16801 tgaggtctat ggaaaatatt atccaaataa tcctcctgca agagctgctt ttgctgtaaa
    16861 aggattgcca ctcgatgcta tgatagaaat tgaaactatt gcagcgaaat cagttaaaag
    16921 ttaaaagtta aaagttaaaa gttaaaagtt aaaagcgatg cactgaacgt agttgaagtg
    16981 ttgaaagttg aaagttgaaa gtcgaaagtt aaaagtataa agttaaaagt ggctgacgcg
    17041 tctattgttt gaaccacaaa agacacaaaa gggcacaaaa ggacacaaaa gttaaaagtt
    17101 tgtaaagtta aaagttcata aaggaatgcc aagcaaaaaa ccgcttgtaa cttttaactt
    17161 ttacctttta acttaatttt ggcaacacaa taactcagtt aaaagtttat aaagttgcaa
    17221 tttttccaag tcaataaaat tgtattgtat tttagaaaat acaaaatata attaaaattg
    17281 tattcttgag aatacaacca cacttgcttg gattaaaact aatttaatat gttttttatt
    17341 ttcaggtttg gaattaaaaa aatattatta tgtttgaagt tttgtaaaca ctataaaatt
    17401 aaactatgat aaagttaagt tataaatttt tatttttact attgtttttg cattccaata
    17461 ttttattatc gcaaaaatat acagctaatg tcccagaaat tgaatataca gaattagatg
    17521 attattattc tgttgggcaa gaagtgcaat gtacagcaag agtatattct tatggtttag
    17581 ttgatgattt atgtgctatt tattataaag tttttaaaga tgatttcaca acgcctattt
    17641 cgagtataga agaatatgga catgcagaat ttgcagttag aggacaagga gaaaattatg
    17701 ttactcaaac tatcagttct ggtagtgggt atataagtgt aaaaccgatg tttacaacct
    17761 acatagcttt tactcttggc atttttgata attggtgtgt taatagaaat cgtcctatag
    17821 ttctatctat gaattttacc gaaccaggac tatataaatt taaagtcgaa attcaaaaat
    17881 gctcaaattc gggttctagt attttaacaa gttttcagtc taatggtaac gaatattgta
    17941 gtactgcggg gacttcacat tccgacaaag caacttcgtc ttgcactagt ccagaaactt
    18001 tgtttactag cgaaataata attccagttt gtagtgaaag tgaaattact tttctcgaag
    18061 gtgaaactga atattgctca ggtgatgaac tttttttaac ttatagactt ggtgataatg
    18121 ataatatgat agatcagtta tcgttgccag actggataag tgtaacgttt gatgaaatag
    18181 caaaaaccgc aagtctttcg ggtataattc cagattatga cgcagaaaac ccaataatag
    18241 aatttgatgt aaaatcaatg aactcatcta atccagatat ttgtcctagt gcagtaatta
    18301 atcaaagtat tacaattctt ccttcagaaa gttcaatcca gaatgttgct atttgccaag
    18361 gtggttctta tacttttttc gatcaagaat ttagcgaggc aggaactcat gaagtagctg
    18421 ttgaaaattc taatggttgt tatgataagc attatttaaa cctaacagta aatcctattt
    18481 ataataattc aacacatatt caactttgtg ttggaagttc ttacgaattt gcaggcgcaa
    18541 cttatactga aaacacaaac attgctcaca catttgaaac tgtttatggt tgtgatagtg
    18601 tagtgaattt gaatttggaa tttgttgaac aatttgaaac aaacttaaaa gatactattt
    18661 gcgaaggtga atattattta tttggtgatg aagaaatttt tgaaacaggt gaatatacac
    18721 ttgaattaag ttcagcacag tcttgcgata gtattgtaat tctaaatctg attgttaacc
    18781 ctacaaatga aataatttta aacgaaacta ttaatgaagg tgaaacattt gtgtttaata
    18841 atcaagaatt aacagagaca ggtgaatatg tatctcattt aattaatgaa tttggttgcg
    18901 atagcactgt tactctaaat ctaactgttg ttacacaagt ttttacaaat gaaattctat
    18961 ataatcaaat ttctgttttt cctaacccaa caaattctat actttatatt aatataggta
    19021 atgttgatga aaatattaaa gctgatattt ttattagcat aacagatatt gcaggtaagg
    19081 aaataataaa taaacagaga tatgtaggta atttagattt atcaacattt aagtcgggaa
    19141 tttattttgt gaatgtttat ttaaaaaact ctaaacttac aacaaaatta ataatgaaaa
    19201 aataagttga ttttacttca atttaaactc ccattttgtt tctttgttag tataatcata
    19261 aatattattg gtttctgtaa attttctgcc acctttttga attgaaatag ttgtatcgtt
    19321 tgctaagttg tgttttttaa catctgttgc aaattctaaa ttaaacaccc aagtaaataa
    19381 gctgttatca tcaaaatcta ttttattttc atcttctata tttttatttt cttctgtatg
    19441 gatttttggt ctaacataag ttattgtttt acattttttc tcaaagtaag atgagtattc
    19501 attagttttt gtatccaaat ttgaataacg aagtgcttgg ttcaatgtat ttatatcggc
    19561 aaaatcgtaa gaaaaataaa cttcgccttc ttctgcatta tttacaattt ttaaattttc
    19621 aattcctaaa atattttcta aaccagactt cacaaaattt aaatattctt caaaatctat
    19681 atttgataaa taattttctt catcagtttt ttcatcaact tctgctttta ggtatttaac
    19741 atattcttta aaatctatag aatatttata cgagcctgaa aaatctttat taaagacaat
    19801 atcttgctga actacacagc taaataaaaa taaaggaata attaataata gtgttttttt
    19861 taatttcata ataataaatt tgaatttgta taataaaaaa tataatttga tgtaaaagta
    19921 aaaatttttt tgtgaaaaga gaaaataaaa acttaaatta gcaaaaaaat aaatctttat
    19981 ggcaagttca cgtattttaa ctttaaatga ggtaattatt caaaaacaat cggcatttcc
    20041 ttatgctaaa ggcgaacttt ctagattgct gatgcacata ggtttagcag ctaagattgt
    20101 aaataataaa atcaacaaag ctggattaga aacaagtatt attggcgaag ccgaccatat
    20161 caatattcaa ggcgaagctc aacaaaaact tgatgtttat gctgatgaag tttttacaaa
    20221 agctttgcaa gcttctggta tcgtttgtgg tatagcttcc gaagaatgcg atgaaatagt
    20281 agtatttgat gacgaaatgt cgaaagaagg aaactatgtt atctgtatgg atccgctaga
    20341 tggtagctca aatattgatg tcaacgtttc tgtaggtaca attttttcga tttatagaag
    20401 gatttcacca gttggagaaa aagttactaa agaagatttt ttacagcccg gaacaaaaca
    20461 agtagcagca ggttatataa tatatggttc atcaactatg ttggtttata ctacaggttt
    20521 aggaacaaca ggttttactt atgacccttc gataggtgag ttttgtatgt cgcattattt
    20581 tatggtaaca ccaaaaaatg ggaaaattta ttctataaac gaaggtaatt atgtgaaatt
    20641 tgaaccaggt gtaaaaaaat atttgagatg gtgccaagaa aatgataaag aaactggtcg
    20701 tcctcttact tcgcgatata taggttcttt agttgctgat tttcatagaa atttgttgaa
    20761 aggtggcata tatttatatc caaaaacagc ttctgcacca aatggaaaac ttagacttat
    20821 gtatgaatgt aatcctatag cttttcttgc agaacaagca ggaggaaagg caacagatgg
    20881 ttatggaaat agaattttag aaattcaacc tacagacatt catcaacgag tgccattttt
    20941 tgtaggctca aaagaaatgg ttgaaaaagc tgaagaattt atgagagaat tcaaagatga
    21001 agagtaaaaa aatatttata atatatttat ttattataat tcctgtattt agtttttctc
    21061 aaaaaattga tcctgtttta attaccactt tagatgaagt agtatccgaa acatcaggat
    21121 taattattta taataacaaa gtttggacac ataacgactc tggtggtgat cctaaattgt
    21181 atagaattga ctctattaac ggctctgttt tagaagaaaa gttaatttta aatgccacaa
    21241 atatagactg ggaagatatt tgccatgatg gagaatattt atatataggt gattttggca
    21301 ataatagtag tggaactaga gaagatttaa aaatttatag aattttactt gcagatttgg
    21361 ataatgaaca acttgattca gtagcatcgg atattattta ttttacttat gatccaagta
    21421 tatatccaga agctatagat aagtctaatg ataccgattt cgactgtgaa gcttttattg
    21481 ccaaagatga tagcttatac cttttttcaa aaaattgggt aaataaaaaa tgttacttgt
    21541 attctttacc aaaagttccg ggtcattata tggctcaacg tttagataca ttggatacac
    21601 aaggtttagt gtgcggagct gattacaatc cagaaacaaa tacaatagca ttaattggct
    21661 atgtgtatgg cattccggca ccgtcatttt tagtcgtttt atccgatttt gataataatg
    21721 atttttttgg tggaaataat ctaagatatg agttgagctt gaacggttat cagacagaag
    21781 caataatttt tcgagacaat gacaggcttt ggttttcaaa tgaaaatttt ttaggacacg
    21841 tgcaatctct ttatgaaatt aaactttcaa cattaggatt aaaagaagaa aatttacatt
    21901 cagtttgtga gatatatcct aatcccacca atgataaaat aagtataatg gtaaatttgc
    21961 ctggaaaaat taaatattct atatcagatg caaaaggaaa ctgcatgata aaaacttcta
    22021 aaaagcaatt tgtaaatcaa gttttagata ttgatgtgtc tttgttgcca gcaggaattt
    22081 attttattga actattaggt cagaattata aaatacaatc aaaatttatt aaactttaat
    22141 tcttttaaga tatttttaag aaaagtaagg ttattttata tttttttttc gcaactttgt
    22201 taactaaaga attattaaat attaaaatta tttttatgaa aaaacttatc tttttattta
    22261 ttagcgtttg gttctcactt gtagcattta caactccaga cgaaggtatg tggctattaa
    22321 ataaaatagc tcaattaaat tatgctgata tgcaaaaact tggttgcaag ttaactgctg
    22381 aagaaattta cagcataaat aattcaagtt tgaaagatgc tatttttcaa ttgcaaaact
    22441 ctcaaggaat gggattttgt acaggtgaaa tagttagtgc taaaggctta ttatttacaa
    22501 accaccactg cggttatgaa gcaattacaa agctaagctc tccagaacat aattatcttg
    22561 atgatggttt ttgggcaaaa tcttatgatg aagagctttc ggctagtgat atgagagtga
    22621 gcagagttgt aagaattgaa gacgttactg ctcgagtatt aaatggagtt acagacgaaa
    22681 cttctgaggg agaaagagaa aaaatggtaa gtaaagcaat gaaagaaatt gctgaagaag
    22741 ccaaaaaaga cactcactac gaagcttctg tttccgagat gtatcaaggt ggcgaatatt
    22801 atctttttgt ttacgaagtg tttggtgatg tacgacttgt aggaacacct cctcaatcaa
    22861 taggaaaatt tggtggagat accgacaact ggatgtggcc ccgtcataca ggcgattttt
    22921 cagtttttag agtttatatg tctccagatg gaaaaccaac aaaaggatat aacaaagata
    22981 atgtaccata tgagccactt cattatcttc cagtatctat agcaggagtg gaagagggag
    23041 attttgcaat gattgtcggc tatccaggac aaacagaaag atatcttagc tcttatggaa
    23101 tgatttataa aagagatttc tttaatccag cagttgttac tattctagaa acacaattaa
    23161 aaactttaaa agttgacatg gatgcagata ttaatgttag attagcaaaa tctgactctt
    23221 atgcatcaag tgcaaatgga cataaattgt tcaagggaga agccctaaca cttaaaacta
    23281 cagatgctat tcaacaaaaa gaacaattag aaaaagattt ccaaaaatgg gtagaaagcg
    23341 acgaagctag aaatgcaaaa tatggaaaag tgttgagtag tttaaaacaa ggttacgaag
    23401 ctttcggacc agcaactctt gatatgattt acttgtcatt aggtctttta caaagtggcg
    23461 ataatgttat gaatgctcaa gcatttatga atcttgctaa tcttttaaaa gatgcaaaaa
    23521 ataatcaaac tgccatagat gctactgttg aaaacttaaa acaaatgagt gaaggaatgt
    23581 ttgacaaata tttcccaaac acagatagaa aagtttttga agcttctcta aaattgtata
    23641 ttgccgatat accagcagaa agacgtaatt ctgtgtttaa taattatatt tttaagaatt
    23701 ttaaagcaaa aactgaagaa gagtcaatag aaaaatttgc tcaagcagtt tatactaaat
    23761 ctatttttac agataaagca agaatggacg cttttttagc aaaaccaagt tataaagctt
    23821 taaattcaga tcctatttat agttttgtta gtaatatttt tacagatgta tttgcaatac
    23881 aaatggcata tgtaggtgca atgaatgata taggtgttaa cgaaagatta tttattgaag
    23941 gtttaagaga atttcaagct aatcgaactt tttatcctga tgcaaactca actttacgtt
    24001 taacatatgg cacagttaat tcttacaaac ctcgtgatgg tgttgattat cactatgtta
    24061 cttattcaaa aggaatttta gaaaaagaag atcctactga tgatgaattt attgttcctg
    24121 ctgagcttaa agaaaaaatt cttaaaaaag attttggtag atatgctgac aaaactggag
    24181 acttaccaat ttgctttttg agcaataatg atattacagg tggaaactca ggctcgccag
    24241 taattaacgg aaaaggtgag ttaatcggct tagcttttga cggaaactgg gagtggttgt
    24301 gtagtaatct gttatttagc acagaactac aacaaacaat taatgttgat gctagatatg
    24361 tggtttgggt aatcgacaaa ctatatgatt gtaagcatat tatgagtgaa cttgatatta
    24421 gaacaactaa gtaaattata acggtgtatt ataagtgtcc aactgtcata ctcagttaaa
    24481 agttaaaagt ccttgcattt gaacacttct aatacacctt gatttttctt tgttatttgg
    24541 aaaattattt tgagaaaaga aaggtgtctc attggttaaa agtaagagct tatgagacac
    24601 cgtctttttt ttattgttgg ctgataaaat aaagtgatac taattcaagt tataatagtg
    24661 atattgaaaa aagtatttgc ataaaaatat gataaattta aaaaaaacga tttttatatt
    24721 attcatagcc ttgagttttg ggcttagagc tggcaatgtg catatatttg gtgaggctaa
    24781 agaatatgct tcaaatacga taatctttta taaatattcg gatagaataa cttttataaa
    24841 aaagcaattg tttgagttag aaatcgataa agatggtaag tttgatgtta attttgaagt
    24901 tgacgatata acatatgtgt ttgcagaatt tggaatttat catgcatatt tttatgcaga
    24961 gcctgatgca agctatgaat tgatattgcc accatttcaa gaaaaatctc tatcagatat
    25021 ttttaatcca ttttttagta gtgaaacaat tcaaatcggg attaaaaata tgcaaccaac
    25081 agatttgaat tatttaattt tagattttga ttattactat tttcgttatt tagatttaaa
    25141 atatattgaa ttatatgtaa atggcttgca aacagatgtt gatactttta ttacagagat
    25201 aaattcaaag tttaataatt acgataatca atattttcaa gattataaaa aatatagaat
    25261 tgctgctcta aaaaatatag caactcaaaa acagtatgaa gaccaaatag tttatagcta
    25321 ttacactaaa gctcctgtgc tatacgataa tcctgcttac atggatttgt ttaataatat
    25381 ttatgctaaa tattttgatt tgttgttagt tgcacctcat ggcgattctc tttacgcaat
    25441 aattacttat gggcatagta tcagtcggtt gaagaaattt ttaagcagaa atttatctct
    25501 tagaaacaat caatttcaag aattggtaat tttaaaaggt cttaacgatg cttttggagg
    25561 aggtaattta caatggttgc cacttttatt gactttagac tctgtacatc tttctacaca
    25621 atatcctttt catcaagatg tggctcaaaa tattgctgat aatgttttaa gcatggcaaa
    25681 aggaacaata gctcctccat ttgaattgcc tgatacagca ggagaaatgc atagcttaaa
    25741 agaatttaaa aataaataca cttatattca atttgcaaat accaaaactt attcttgtca
    25801 gttggaattt gctatgatgg gaaatcttca caaacgatat aaagaagtat gtaattttgt
    25861 aactattctt acagacagcg atattgaaag tgccaaagca tatattaata aaaacgaatt
    25921 tggctggaca tttttattta cggaaataaa ttcacaaaca atatctactt ataaaattga
    25981 tacataccca acttattttt tagttgatac acgtggaact ttattaatgt ctccagcacc
    26041 atcgccatca gaaaattttg agaaatattt cttcaaatat gttgaagata aattacctaa
    26101 aaaataattt tttaatgtgc ttctaaccaa ttgtcggcta ctccaatttc tacaattagt
    26161 ggaactttga gtgttacaac attttgcatt tcgtccttaa caatttgttt tacttgttct
    26221 aattcgggtt tgtaaacatc aaagttgagc tcgtcgtgga cttgcaatat catttttgat
    26281 tttaaattgt tttcttcaaa ttttttgcaa atgtttacca tagccaattt tataatatct
    26341 gctgctgagc cttgtattgg agcatttata gcatttcttt cagcataaga tctgactatt
    26401 gcattgttac tatttatatc ggctaatttt ctacgtcttc caaaaattgt ttctacatat
    26461 tgattttcac gtgcttttgc tatgcttgct tccatataag cttttacgcc agggaaagtt
    26521 tcaaaataat tgtcaattaa agttttagca tcagtacgtg aaatatttaa gcgttgtgct
    26581 aaaccaaagg ctgaaattcc ataaataatt ccaaagtttg cagtttttgc ttgatttctc
    26641 atttcgcgtg taacattttc agcatcaact ttaaatattt gagcagctgt ggcagagtga
    26701 atatctgtac taaatttatt aaaagcgtct aacatatttt tgtcttcgct tacatgagcc
    26761 ataattctta actcaatttg agaatagtca gcagataaaa atacatgctc agaatcagaa
    26821 gcaacaaaag ctttacgtag ttctctacct ctttcgtctc gaataggaat gttttgtaaa
    26881 tttggattat tagaacttaa acgacctgtg cttgtaactg tttgattgaa acttgtgtga
    26941 attttgtttg tttttggatt tactagttgc ggcaaaacat ctacataagt atttattaat
    27001 ttattaatac ttctaaattc caaaatttca ttgatgatag gatggaggtc tttaagtttt
    27061 aaaagttctt cttcgccagt gctatattgt tttgtttttg tttttttagg atttgaaata
    27121 atttttaatt tttcaaaaag aataattcct aactgctttg gagaagctat gttaaatgtc
    27181 tctccagcaa gttcataaat tttttcttct atttgtaaaa gttcttttct taaaatttgc
    27241 gattgctctt ttactatttc tacatctaaa ctaactccag ttttttccat gttggcaaga
    27301 acttttacta atggcatttc aacttcaaaa aatagaggtt ctaaattatt ttcttttaaa
    27361 ataggagtga atatattttt aagttttaaa gtaatatcag catcttcgca agcataaaaa
    27421 ctgagtaatt tagtgtcaac atctctgata tgtttttcat tttttccttt gccaaccaaa
    27481 tcttcatacg aaatcattgt ataattcaaa taagtagccg caagaaaatc tattccatgt
    27541 ttttgttctg gttcgattaa gtaatgagca atcattgtat caaacaattg tgcttttaca
    27601 ttaattccat atttatctaa aattgctaaa tcaaatttta aattctgtcc aatgattact
    27661 ttttctttat tttgaaaaat tggattaaac atttctaaaa tctctttagc agtatcaaaa
    27721 ccttgaggaa gaataataaa ataagcttga ttttcgacaa ctgataaaga aattccaaca
    27781 atttcggctt gcattatatc aagacttgta gtttctgtat ctaaacaaat ttctttgtgt
    27841 tcgtttagtt ttgggattaa ttcttcaatt tcgtaaagag tttctataag tttataatca
    27901 acatctctat tctctgttgt aatgtcaggt atttcagttt gagctgtttc agaatttgaa
    27961 actgaagaaa ataaatctaa ttgcagattg ccttggtttg gaactgtttt ttgttttact
    28021 tgcttaatat tttcaccaat aactctgttt tttaaagttc taaattctaa ttcatcaaaa
    28081 atttggttaa gttttacaaa gtcaggctct tctaatttta gctcttccat atttgtttct
    28141 acaggcactt ttgtgtcaat agtaaccaaa tgtcgagaca gatataattg ttctttgttt
    28201 ttttctaagc tttctttttg tttgccaact attttgtgaa tgtttgcata aatgctgtcg
    28261 atatttccaa atttatcaat taatttactg gcagtttttt cgccaattcc tggaactccg
    28321 ggaacattat cagatgtgtc tccccacaat gctaaaatat ctattacttg ttcgggattg
    28381 ctaactccat attgttgatt aagttctgtt agaccaataa cttccgcagc ttttccaccg
    28441 cgtgcaggct taaacataaa gatgttttca tcaattaatt gagcataatc tttatcggga
    28501 gtcatcataa aaacatcgtt ttcggcattt ccaaattttt tggcaagtgt tccaataaca
    28561 tcatcggctt cgtaaccact tacttgaata acaggaattt tgtatgcttc tagaatttgc
    28621 ttaatgtatg gaacagaaag tttaatgtct tcgggtgttg cttctctgtt agctttgtat
    28681 tcaggaaaaa tttcatgtct aaaatttggt ccagaagggt cgaaaacaac tgcgatatga
    28741 cttggttttt ctttgtttaa aacttcttct aaagtattca caaatccaaa aattgcagat
    28801 gtgtttaagc cttttgaatt aatacgtgga ttttttataa aagcataata cgacctgaaa
    28861 atcagtgcgt aagcatctaa gagaaatatt ttgtttttca ttcgttaaag ttttgtccaa
    28921 agttaagaga aaaaagctaa atattgaaat ttgttgtaaa atttaatagt cctttgtgtt
    28981 ggttttgtca tgatttttgc aacaagttgg caaaaatccc gccaaaaccc aaactacatt
    29041 tccaaataca aatcaataag tccttcgggt gcagagattt gtatttcttt tttctcgtca
    29101 ttaatttcga gaataatatc ttccgcgtaa gggataatta taattttttc tttaaaattt
    29161 atttctatca caggatttcc aggaatattg ttaagcatgc acacttgacc gatgaatcca
    29221 aattttttat cataaacact atagccttca atgtctttct cgttatcttc atcttcgtca
    29281 aaatcgtcaa acaaggattt taaagtcata atttctgttc cgacaaattt ttctgctttt
    29341 tctaaagatt ctatatgccg taatttaacg acaacatttt taccttggct tttactgtgt
    29401 tctataaaaa aaggaaccgg cattccatca ataatcagga aaaccggtaa atcttctgtg
    29461 aaaatacttt tatcgaaatc ttggctgatg tttaataaaa ctttgccgtc aactccgtgt
    29521 gttctttgga tttctgcaat ttttattaat tcttccttac caaaaaacat aattactcct
    29581 gagtattctc agttgtttct tcagcttttt cttcttcagc tttttcttca actttagctt
    29641 ctactttttc ttcagcttta gcttctggtg cttctgcttt tgcttcttca gttgcttctt
    29701 ctgctttttc agcttcttga gctgctttag ctgcttctgc ttcagctgcg aatttcttag
    29761 caagttcttg agctttttgt tctttaactt tacgttcagc ttctaatctt gctttatcag
    29821 cttcgcgttg tgctaattct aagttagaaa ctttagaaga aattttagct tctttttctg
    29881 ctaaccaagc tttgaaacgt ttttcagctt cttcttcgtt aaaagctcct tttttaacac
    29941 cttctaacaa gtgttttttc attagaactc ctttgtaaga taaaattctt cttactgtgt
    30001 cgcttggttg tgctccattc attaaccaag ttaatgctct atcgaaatct aattcgattt
    30061 tagcagggtt acttacagga ctgtaagaac caattctttc aataaattta ccatcacgtg
    30121 gtgcgcggct gtcggctgca acaatgtaat aataaggttg tcttttgcgt ccgtgtcttg
    30181 ccaatcttaa ttttactgcc ataagataat taattaaatt attaataaat atcggtttct
    30241 cgaaccgagg tgcaaagata agctattatt tttaattatt gaattatttt ttttattttt
    30301 gactttcaac ttaattttaa acttgttttt aaaagtttta tcgtaattgc aatggtttaa
    30361 aagggttaaa actgaaaaat aattttaatg ttttataaaa aaataataaa aatagcaatt
    30421 tttctgacga ttttagctgg aacaacatcg tgttatcgcg aagaaattgt attaattccc
    30481 gaaacagatg acaatttaca attagaatcg cttttatatt ttaactcaaa accttgcttt
    30541 tttgacgaag caacaaatac tttaacttat agtatagttg aaacagaact tcaaaatttt
    30601 aatccaaaac ttagttttag tccattagta aaagtgtatt ataaaggaaa attattaaaa
    30661 aattcagagt atttgcagtc tgattctgag aattttgaat ataaaaaagc atatagtttt
    30721 tattttgtta caaaaaatga ttctgttgaa atacaattaa ggtttacacc tttaccgata
    30781 gtcaggcttt caaagacttt gacaattttt gatgaaccta aaattccagc ttattttcaa
    30841 attgtatatc ccgaaagttt taaatcagat tattcttgtt atataggtgt ggaaattaga
    30901 ggtgcaactt ctcaaattta tcctaaaaaa agtttaggct tcaacactct tgctaatcaa
    30961 gatattaagt cttaccaaat gactaccctt gttgggaata gagaaaattg gaaatggatt
    31021 ttagatgctg cttatatgga taatttaaga gttagaaatt ctgtgtcgtt ttacgtttgg
    31081 gcaaaaatta gtaattctcc aaatcatttt tcaataaata ctacacctgt tgaattgttt
    31141 ataaacaatg agcatcaagg tttatatttc ttaggtgaaa attttagtaa cgaaatttta
    31201 aatttaaaag caaattctga agttctttac aaggcttatg attggagcga aggtgcaaca
    31261 agatttgaat ttgctaatga taatatttca aaaaatgagt tttgggacgg ctgggaacaa
    31321 aaacatcctg acccaaaatt ttatctaaat tggaatccat taaaagattt acgtaacttt
    31381 attgtaaatt caagtgatga gaactttcga gactcaatat cttattatat tgatattgat
    31441 aattttattg attattactt gtttatgaac ttattgtttg ctcttgataa tgctggtaaa
    31501 aatacttttt taataaaatc tgctcagaca ggaaaatttg tgattattcc ttgggattta
    31561 gatgcaactt ggggattatt ttgggacgga acttataatg aaccaacatt cataactact
    31621 aattatctgt atgatagact ttttgaatta aatccaaatg attttaaaac taaattaaaa
    31681 aatagatggt ttgccttgcg tcagaatgtt ttttcgcaag aagaaatttt actagaattt
    31741 gaaaaacctt tgagcttgat attaaaatct ggaattattg aaaaagaaaa tttaatttgg
    31801 aaattaaata ttaatacaga agcagaatat aattatatca aaacttggat atcagcacgg
    31861 cttaattatt tagataatta tttttcaaat ttaagttgac tctataatag ttcactaaat
    31921 gcgaatggat tatactacac tttttttaaa aagagaagaa attgtcctga aaactcttga
    31981 aaacaaagta aggcggaaat aaattttatt attaaagatg aaaagatttg tttttttatt
    32041 aactattgct ataattacga ttacgcatgt gaaagctcaa aactataaag ttggggtttc
    32101 attagaaaat attgttagtg attttaactg gaacaaacct ttagaagatt ttatggtaac
    32161 tatacaacca ttttcattag aaactaaaat gccaataaaa cttggtgttt gtgttcaaag
    32221 aaattttaaa gtaatttcgc catctgttca tgtcaatgta atttacagaa atattaaata
    32281 tgcgaaacac tatacctcta ttacaaattt taatcattat tcgctaaatg ttccgataaa
    32341 cttaaattat cgtaaacgcg taagcgatga gtcaactttt gttgctaata ttggtggtgg
    32401 aataaattat atattaactt caaatgataa aacaaactct gggatgtata atgatggaga
    32461 ttta
//
LOCUS       DAOPNX010000020        31187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_1891_length_31187_cov_6.072562, whole genome shotgun sequence.
ACCESSION   DAOPNX010000020 DAOPNX010000000
VERSION     DAOPNX010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 31187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31187
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1891_length_31187_cov_6.072562"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2897
                     /locus_tag="PKN53_03630"
     CDS             <1..2897
                     /locus_tag="PKN53_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOD88075.1"
                     /translation="VLNATITEPSLLQASSSATAIACYGGTSTVTVSASGGTGSYTGT
                     GTFTVSSGDYSYTVTDANGCTATTSVTITDPPQLIASSTFTPVLCNGGISTVTVSATG
                     GTGSYSGIGEHITYAGAYSYTVTDSNGCSSVTSGTVTQPALLVASSVANDILCNGGTT
                     SVTVTATGGTGSYTGTGVMTNIGAGTHSFTVTDGNNCSSVTSITISQPSLLVASSSAG
                     NILCNGGTTSVTVSATGGTGTYAGTGVITNIGAGTHSFTVTDANNCSSVTSITISEPP
                     LLVASSSAGDILCYGGTTTVNVTATGGTGAYTGVGSIPNVSSGTHSYVVTDANGCSAT
                     TQITINQPDMLIVTAVVDNHVSCNGLSDAQATASTTGGTEPYVFHWNDPSSSYGNTVH
                     QLPAGTWTVLVTDSNGCTVTKSVTVTEPLVLSVNINTVDVLCFGESTGSANAVVTGGT
                     YPFTYSWSNSGTTSSISNLSSGVYVLTVTDNQGCQAISNSVINQPPELNVYLTSSSAI
                     CGSTGGSVLASVIGGSPDYQYIWSVSGSQNNIQNLNPGTYSVTVHDSHMCSKTQSISV
                     DVVGNIDADINVLSYISCFGQKDGALQALSSNGNTPLNFEWNTGAESSTLTNLGSGSY
                     SVIITDSWGCIGSANYVMIEPTEIVVDANIQGTSCFGYADGAITTQVSGGSSPYNYLW
                     SNAQTSSNISNLVAGSYSLTIYDKFGCQQIKTYALTQPDKLEIDLDIKDISCFGNIDG
                     GVKLSATGGTQPYSYALGIQDSIYAGQMHSPLRTGSYVAYVRDINLCIDSSLFMIVSP
                     APLEASYVFENPSCIGARNGSIEIQVAGGVEPYLFETNDAVVDIPLLSGLSAGNYNVI
                     ITDANDCSLELKNIVLSESDVECVKIPNAFTPNGDGVNDTWIIENLEMFPGARIFVYN
                     RWGQEVWIGYPGDEWDGKHNSKLMPATTYLYVIELYDGSKPYTGTVTIVY"
     gene            3021..4193
                     /locus_tag="PKN53_03635"
     CDS             3021..4193
                     /locus_tag="PKN53_03635"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOD88076.1"
                     /translation="MKKVIDTEKIPIKLWLDDIDENTLQQAKNLANLPFAFKHICLMP
                     DAHSGYGMPIGGVMAADGVIVPNAVGVDIGCGMCAVKTDLKFSPSIRKKMESILGDIR
                     RKVPVGFKHHKRAQEEDLMPKGYGIDNMEVVRAEYKSALKQLGTLGGGNHFIEIQKDS
                     ENMVWIMIHSGSRNIGKQVADHYNKVAKSLNQQWNSPVPPKADLAYLPFDTGEAHRYF
                     EEMNYCVDFALANRKLMLIRVQEVLLDYFPNLNFGETINIAHNYATWENHFGKDVVVH
                     RKGATSAHKGKLGIIPGSQGTNSYIVEGLGNPESFSSCSHGAGRKMGRNEAVRSLSLE
                     DEIRKLDKKGIVHAIRGKRDLEEAPSAYKNIVQVMANQEDLVKIKVELSPLAVVKG"
     gene            complement(4213..4656)
                     /locus_tag="PKN53_03640"
     CDS             complement(4213..4656)
                     /locus_tag="PKN53_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88077.1"
                     /translation="MKKNILDFTFGVIGVILGILILAIGIYVSFSSWLVGLTIIIVGA
                     FLAFSHNACFIDFKNKRVKFAALLFGLIKIGYWTNVKEDMYIKIIGSGNESKNENNEK
                     TFEKYKISLFSSENNEILVLLKSKQIDKINNFSNHFSKEFNIPIK"
     gene            complement(4692..8939)
                     /locus_tag="PKN53_03645"
     CDS             complement(4692..8939)
                     /locus_tag="PKN53_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOD88078.1"
                     /translation="MKNQIHTYLISVKIILALFLTTSSLFAQDNYIYFTPSSDTVLIE
                     SIKVENLRTNNTLSIEPNATLHIVSGANLKFMALEPQKFKVFPNPSESNFNISYFAEN
                     SGTVNFTIFNISGAVVYKNSFYNKKAYHQLELNAKLSGIYFIQISGDTFSYYIKVIGT
                     ADETYIEGIRHANSIDIENENTENDESNIYLKQSLENTLAYTFGDVLLFTFTSNEGDI
                     TKKTLVINQGDYYDGEYIDLEVSFYRCIDIEGKHYPVIVIANRIWMAENLNTKYFINN
                     NEITTITDNAEWRKTSKPAYSIYANNANNSGIYGKLYNWYAVNNEVPMCPKGWHIPSD
                     ADWKIIENLIEVQNIDTIGWRASNSACYLKSATQGIWDLPDKKLVNSTGFTAMPGGMR
                     ALTGEFHYKDDYAAWWTSSEQNEKNAWCRALDNSSCNIFREHGSKSLGLSVRCVYDKK
                     AVEEVPTLGNVQADYYSENASSAYFFVEIINHGLSPITEGGFMVSENPNFDNDCDDDC
                     DIYYAQDIYDHEIMLFIDSIEPNQTFYVKAFAKNSYGTGYSEEITFSTIPGNPKLGTI
                     EANFNAADGNVDFHVVIIEQGVSSIYEGGFMVSSTPNLDFSNSDIYYAQEIFENEFMV
                     HLDNIATNQTFYVKAFARNHHGTGYSEEISFSTTIPEPIIEPSIEILQIDSVYMKSAR
                     ISGHVESEEELYNLGVAWDKNPNFTIGESGIHYLPSNFTQSDYSYVINNLEPNKIYYV
                     KTFAQNSGGQSITNEFSFTTLEKYYISESTVTDIDGNVYRTVRIANTEYMTENLRTSR
                     LNDGTLIPLVDQNWESSPSMKSCYMDFDSVNNHLKNGKLYDQFAAQNEYICPTGWKVP
                     EDNDWVKLEMLLGMSETDALNTTGWRGTNQGGLLKQVELDNWISPNNGATNQTGYSAQ
                     GSGYITESGEFVGKGEIFSTWVNSENSTFVRTLSNAEQRIYRDEYSTPSSNTNGYSIR
                     CVRTNIGTPTVTTITPNNISISTADVSGEIVDEGESPISEKGILWGTWSGITLESCIG
                     FISAGAGSEQFTSTISGLNSNQNYYVRAYATNNNGTSYGEAIEFQTLNKLYVEGPGVL
                     DWDGNLYKTIVINDREWMAENLRVTKKNVSTDTTLMLISLQHVQSLEEWLTFVHGEEK
                     PSYCKYENSTLSNDSTYGYLYNFKAAESNIYNGNGVCPSGWVVPSDDDWFYLELFLGM
                     EEFEALNEGWRGTDEGGMLKEAGTENWLAPNLGATNTAGLNFRPAGYRDSTNVSMFSE
                     RGETLNIWTSTEITGEANAVAYSRHLKFDSSQIGKEKSGKNHGFSIRCIKQNGKSTVI
                     LHSADYDEVASEISLQAEVLIDGAYEIVEKGFFWSFSSAPHAENYDGVYTAGGMGGGS
                     FNHIISATPSIAYHIKAYVKLANDTYEYSNPIVVVTY"
     gene            complement(8951..9622)
                     /locus_tag="PKN53_03650"
     CDS             complement(8951..9622)
                     /locus_tag="PKN53_03650"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOD88079.1"
                     /translation="MKRLSLLFCFIICAFMLNAQGFYYGITAGSTMAKIIEKTEIGQD
                     YKKSNKFGYQVGLAAEYEILSFLYVGASLNYITKGDKYKDNFALSKINFGSFEVPLQI
                     GYKIPIGNVTVSAMVGPYTNVAIVGKRTFIPREEIVPPFEWNFEDTPHAQYLDNEDDV
                     FGEKWSSYKRFDSGICAGLRVGFKNYNVSANYSRGFVNIRHDETITANHSVFNLSFIY
                     YFNAY"
     gene            9814..11601
                     /gene="lepA"
                     /locus_tag="PKN53_03655"
     CDS             9814..11601
                     /gene="lepA"
                     /locus_tag="PKN53_03655"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOD88080.1"
                     /translation="MKHIRNFCIIAHIDHGKSTLSDRLLQYTDTVAERDFQNQLLDNM
                     DLERERGITIKSHAIQMEYEHEGENYILNLIDTPGHVDFSYEVSRSIAACEGALLIVD
                     ATQGIQAQTISVLYQAIENDLEIIPVLNKMDLEAAMPEEVEGQIIDLIGCAREDIIHA
                     SGKTGYGVDKILDAVINKVPAPEGDDQAPLQALIFDSVFNSYRGIIAYFRIFNGKIST
                     KDLVKFVNTGKEYDADEIGVLKLGLSPRETLSAGDVGYIISGIKTAAEVKVGDTITHI
                     ERPCEKAIEGFSEVKPMLFAGIYPVDADDYEELRASIEKLQLNDASLTFTLESSAALG
                     FGFRCGFLGLLHMEIVQERLDREFNMDVITTVPNVQYLAYTTKGEQVVVYNPSGMPSP
                     SHLDYVEEPYIKGSVITRAEYVGPIIKLCLDKRGTLLNQVYLATDRVELSFELPLGEI
                     VFDFYDKLKSISRGYASFDYHQSGYKESKLVRLDILLNGEMVDALSTLTHIDNAQGFG
                     RRMCEKLRELIPRQQFDVAIQAAIGSKIIARETVKALRKDVTAKCYGGDITRKRKLLE
                     KQKAGKKRMRQIGSVEVPQSAFLAVLKLD"
     gene            complement(11608..12258)
                     /locus_tag="PKN53_03660"
     CDS             complement(11608..12258)
                     /locus_tag="PKN53_03660"
                     /inference="COORDINATES: protein motif:HMM:NF027898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOD88081.1"
                     /translation="MMKKIILVLSIILFYANSFSQNDAAATALLDEIAAKADRYTSLK
                     VDFKMFVENLQTKKRDSYSGSAAYKAGYYKLDIMGQIVHSDGKTNWTYLKDAEEINIT
                     DNSENQADMMNPKTILKDYKNKFKIKYISDKFEANRPLVEIDMYPKVIEDKKYSKITL
                     KVDKTKKQIYSARYVGKDGVSYLIEIQKFLENPSIPDTEIKYSNNLYPDAEVIDMR"
     gene            complement(12279..14705)
                     /locus_tag="PKN53_03665"
     CDS             complement(12279..14705)
                     /locus_tag="PKN53_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOD88082.1"
                     /translation="MAKKSKNTKEKKEKKSLKDNNIIRFFKDEKFRISLGLLLLGFSI
                     LLCLSFTSYLFTWKADQSMLGVGFQGLFTNSDFSVENWMGKIGALISNHFIYNWFGLP
                     SFSFVLIFGVFGLRLLNIKLTNVWRFLFVSLIFTIWLSVALAAIFKDNYFMVGGSHGY
                     FIYLWLNALVGEIGTYTILIITAIIITIYTFKNSLNWFKIKINDIKTIKSDGLLEDNS
                     DNFKQDLEEIGKENDEDDETEKTDENSEMIPNVVDFDNEDEKDDDENEKEEAKPEEVV
                     TEENLQLAINEQTEEEALSDDEINSDFIGDYDPTRDLEYYKMPPIELLKDYPTGKSDV
                     TRDELNANKNRIVSTLQDYKIEISRITATIGPTVTLYEIVPAPGVRISKIKNLEDDIA
                     LSLAALGIRIIAPIPGKGTIGIEVPNSKPEIVSMRSVIKSVKFQESNYELPVAIGKTI
                     SNETFVFNLAKTPHLLIAGATGQGKSVGINAIITSLLYKKHPSQLKFVLVDPKMVELS
                     VYRRIEKHYLAKLPGEENPIITDVSKVVPAMNSLCIEMDRRYTLLTQAGVRNIIEYNE
                     KFIARKLNPNKGHFFMPYIVVVIDEFADIIVQEGKQVEAPISRLAAKARAIGIHLIVA
                     TQRPSTDVITGVIKANFPTRIAFKVTNGHDSKTILDSTGANQLIGRGDMLISGTGKLT
                     RIQCAFVDTPEIELVVDHISKQQSYPNVFELPEPEIAEGSSGVGAINPDERDEFFEEA
                     ARLIVRHQQGSTSFIQRRMSIGYNRAGRIMDQLEAANIVGPNEGSKARQVLFPDEYSL
                     EQYLNSLSNK"
     gene            complement(14786..15460)
                     /locus_tag="PKN53_03670"
     CDS             complement(14786..15460)
                     /locus_tag="PKN53_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88083.1"
                     /translation="MKTTFKLLLVLVAVSLSLSSFSQKKAKPFRGTVTYDITYDSEEE
                     LDEMTKAQLPTNIITYISGNKVRTDQVSAFYSMASISDFDKGGVIILMDAMGMKIAMK
                     QEKEDIDKALSEADMKDPEIKFIDETKMIAGYKCKKAEVTNGEDVVEIYYTDEFNVPE
                     KINELNGFKGIKGILMEYSMTQQGLTMTMTAKELKAGKVKGGLFVIPDDYELKTPEEL
                     GGMFGQ"
     gene            complement(15613..18393)
                     /gene="leuS"
                     /locus_tag="PKN53_03675"
     CDS             complement(15613..18393)
                     /gene="leuS"
                     /locus_tag="PKN53_03675"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOD88084.1"
                     /translation="MEYNFKEIEKKWQAYWKENKTYKVEIDNKKPKYYVLDMFPYPSG
                     SGLHVGHPLGYIASDIYSRYKKLKGYNVLHPMGYDSFGLPAEQYAIQTGTHPAITTEK
                     NIKRYREQLDNLGFSYDWDREVRTSDPKYYKWTQWTVVQLFEHYYDTKLNKANSIKHL
                     TEIFATQGSKAVSAYCDEHEDFTAEQWNTMSKEEKEEVLMKYRLLFLADTTVNWCPKL
                     KTVLANDEVADGLSVRGGFPVEKKKMKQWSLRITAYADRLLEGLQTIDWPEPVKDIQT
                     NWIGRSEGVQMFFDIKDSDKKIEIFTTRPDTIFGVSFMVLAPEHELCSELTSAENKVK
                     VEEYINWAKNRSEVERMTEKKVSGQFTGSYAINPFTKQEIPIYIADYVLIGYGTGAIM
                     AVPAHDSRDFAFAKHFNLNITQVIKNPNEKIIPTSEWTESYDSKDGILINSDFINGLE
                     VKEAISKMNSKISELGLGKVKINYRLRDAIFSRQRYWGEPFPIYYKNGIPKTMSIDEL
                     PLELPDIDKYLPTDDGEPPLARAKNWQTADGHKLETSTMPGFAGSSAYYLRYMDPNND
                     NELVSEEAVNYWQNVDLYIGGREHATGHLLYSRFWNMFLYDLGLVPNKEPFKKLINQG
                     MIQGRSNFVYRINGTNKFVSYNLKDQYETQRLHVDINIVHNDILDIESFKKWQPEFKD
                     AEFILEADGKYHCSWEIEKMSKSMHNVQNPDELVEKYGADTLRMYEMFLGPIEQAKPW
                     DTNGIEGVFRFIRKFWRLFHNDNDEFFVSDDKANAAELKILHNTIKKLSDDIEKFAFN
                     TGVSALMICINDLGKCNKREILEPLCILMSPYAPHITEELWNLLGHKESITKAMWTTY
                     NPAYTVENSFEYPISFNGKTRFTLELPLDLDNKQIEEAVLSNENTQRWIEGKQIVKMI
                     IVPKKIVNVVIK"
     gene            18522..19508
                     /locus_tag="PKN53_03680"
     CDS             18522..19508
                     /locus_tag="PKN53_03680"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HOD88085.1"
                     /translation="MKKSVILISLLSIVLYSCNSGSYKTVAYAHGQAGDIIVVMNNQA
                     WDTEAGDSLRKVFHEYVSGVPMEEHMFDLHQIPREQFVKGNKLHRNVIYQEVNSSIEQ
                     ASIKISRNKFAMKQVFVEIAAPNQHEFAKIVAQNKKKLVDIFLEADKARWIETLTKYT
                     NKIASKRVQERFDIFLRIPSYYNIEEDRDGFMWICKEAKNYSTNILIYSWEKSDTSVL
                     TAEYLISMRNLILQNNIPGEKPNSYMTTETKYDYPEFSLLMHNSMQTGKLKGLWRVHG
                     DFMGGPFVSYTKIDEPRNRLVTVEGFVYRPNQEVRDLIRELEWTCSTFDITF"
     gene            19572..20060
                     /gene="ssb"
                     /locus_tag="PKN53_03685"
     CDS             19572..20060
                     /gene="ssb"
                     /locus_tag="PKN53_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOD88086.1"
                     /translation="MNLNKVMLIGRLGKKPEVNYIQPDLPVAKFSLATSEYYKDKTGA
                     KIENTEWHNIVAWRGNAKFAENYLNKGMLVYIEGKIQTRRWESQDGTARYVTEIVADI
                     LRILEKRENREFTQNSQTQNTEANSKSSFDESSKQESQDFMADDDVFGGDDLGNNDIL
                     PF"
     gene            20080..21414
                     /gene="gldE"
                     /locus_tag="PKN53_03690"
     CDS             20080..21414
                     /gene="gldE"
                     /locus_tag="PKN53_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HOD88087.1"
                     /translation="MDPEPFINSIILLNAAFDTKVVIIEAIAIVVLLFCSGMISASEV
                     AFFSLSAKDLHKLKKTSPKSFERIVRQLERPNWLLSTILIANNFVNITIIILSTYLTQ
                     YLFAGMDTNSVAYILLQLVLITFLILLFGEIMPKILAAKIPVKAAKAMSLPLLILSII
                     FYPISYLLIHSTNFINKRLEKKHKNKISIEELSHVIELAGEDLKEDKEMLEGIVNSIN
                     LSAKEIMTSRIDVFALDYNLTFSKVVEGIVNSGFSRIPVYVDNLDNIKGVLYIKDVLP
                     YIYLQNKDDFKWQKLIKPHFIVPESKNINELLKNFQDKKLHIAIVVDEYGGVSGLVTL
                     EDVLEEFVGEINDESDEDEETLFEKIDDKAYVFDAKISLVDFCKIIDVDYSIFNEIKG
                     ESDSLAGLILEVNGEIPKQNKVLEIAGFLFTIIDSDERKIIKIKTKLPDEVQ"
     gene            21401..21970
                     /gene="gldD"
                     /locus_tag="PKN53_03695"
     CDS             21401..21970
                     /gene="gldD"
                     /locus_tag="PKN53_03695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HOD88088.1"
                     /translation="MKFNKIHILFFLIAFIFISCKDKNTIPKPRAYFRIDFPEKAYKQ
                     YDDDCPFKFEIPVYSFLIKNKEENCWMDIYFPKNRATVYMTYKELDDNLRQYLEDQRE
                     FVYKHVVKADAIEETEFINYDDKVYGMLYNIKGNTASSVQFYLTDSVNHFLRGSLYFD
                     VKPNKDSLAPVINFIRGDIEHFMESFSWK"
     gene            21978..22601
                     /locus_tag="PKN53_03700"
     CDS             21978..22601
                     /locus_tag="PKN53_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOD88089.1"
                     /translation="MLKNFVQGKAISGIKEISKDLNYLYSYCDLTAVDNMLLMQSGSL
                     ARRLEILNTRALIKDCGLKLSIIYKNRKPIVENGFVSISHSDSLVAFVWNPDFEISID
                     IEEIGERITRIARRAFSEDELKFANDDIVKLNLLWNVKECVYKLAGIKGLEFKTQIHV
                     FPFENIENIEAELITSEGKRNFLFHGKILKEHSLVYGSEKVKTFNRI"
     gene            complement(22744..23787)
                     /locus_tag="PKN53_03705"
     CDS             complement(22744..23787)
                     /locus_tag="PKN53_03705"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOD88090.1"
                     /translation="MDKEHLEPQVNEEEFEQSEELFEHHRLVIEPGQKLMRLDKYLVC
                     RLASTSRNRIQNAAEAGNILVNGKPSKNSYKVKPGDIISIVLSFPKTETELIAQDIPI
                     NIVYEDDDFVIVNKNPGMVVHPSFGHYEGTLMNALMFKFQQLPSTPGNEIRPGLLHRI
                     DKDTSGLLVVAKNEIAMNKIAKQFFDHTTKRKYQALVWGNLANDEGTITGHVGRNLKN
                     RKIMDVFPDGEYGKEAITHYKVLERFNYVNLVECQLETGRTHQIRVHFKSIGHPLFND
                     WEYGGDKILKGTTFNKYKQFVENCFKICPRQALHAKTLGFIHPSTGKEVCFDSDLPED
                     MQNCINKWREYIS"
     gene            complement(23790..25646)
                     /locus_tag="PKN53_03710"
     CDS             complement(23790..25646)
                     /locus_tag="PKN53_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD88091.1"
                     /translation="MMKKLSFLYISILISVISIAQEYTVDYGNMFLQQYSRNYFNKIS
                     LKNDPVVDTLELPFFDDFSASYVYPDSTKWLDKYAFINNTYAINPMTYMTATLDAADE
                     FGNIYTHLPSLSSAIADYLTSKPINLAVSPSDSVYFSFFYQAGGNGNKPEAGDSLVVQ
                     FKTPDLPWRSVWHVAGGNAMSEFKNVLIPITDTLLLVKGFQFRFLNYASISSNYEPSW
                     ISNCDHWNIDFVMLDTARTYKDTIANDVAFIKNFGSMLNGFEAVPWKHYKANHSGLIN
                     DTLTWVYRNTGPDIFAINRQIEIIDLYGNNSGFSMNDDNENIQAFETISYSRRIPYVF
                     NSNSEDSARFLLKGYITTDDAAERFRYRWNDTIRYYQKFENYYAYDDGTAEKGYGISG
                     QNTKYASLAYQFKPITNGRLYGVYIYFNKVLNEGNKKYFFLNVWEDNGGVPGDTIFQK
                     LGVLPEYSDEINGFVYYPLDTVLNINDVFYIGWTKTTDDMLNCGFDIHNDASQHLFYN
                     VDGTWQNSSIFGALMVRPVFEYEIKKQQSNVLVDNFVEFQIYPNPANEFINIESFSDN
                     DIVQIYDFTGKLILSSKVKSQIDISALSSGMYVVKLNSNLANYKTQKLLIAR"
     gene            complement(25653..26519)
                     /locus_tag="PKN53_03715"
     CDS             complement(25653..26519)
                     /locus_tag="PKN53_03715"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOD88092.1"
                     /translation="MKKAKGKNKFLNFLKSKLFIFNVLAAIALVIIIVIIVNFSLKIY
                     TKHGKSVTVPEFVGEQADKALASIEENGFQYVIVDTVFDDKFPKGAITEQNPEAGSLV
                     KSGRKIYVKINSQETEQISMPQFIGMSVKMVNSMAENYGLKVGNLKYVPDISVNVVIR
                     QLYRGRDIEPGIKIKKGATIDLVLGLGLSDKTTNVPTLIGLTKKEASNLLLDLYLNIG
                     AVVYDNTVRSSQDSTNAKIYRQSPRASTINQVNLGYSVDIWLTKDQSVIDEAIDETNA
                     KLSEEENENTDE"
     gene            complement(26652..30326)
                     /locus_tag="PKN53_03720"
     CDS             complement(26652..30326)
                     /locus_tag="PKN53_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOD88093.1"
                     /translation="MNKKLHKIILSACLIFSTALFNYSFACSGSTLAGNLTPTDNWQT
                     ISGVQAGDRYTFTLAAGEVIIFSFCQGGGSYTNDPMIDLHNADGSLTYQSNDDHCGYG
                     SELVWVCPTSGTYSIGLYNFYCSTDGTALGTVAFKKLPTPTEQDCLGARPLCASVSNH
                     PQSYVGTGHYYDLFNFNEHQGMAVSTHNCPNCLVTGEKNNVWYTFTAQTSGELAFTIT
                     PYNSSDDYDWAVYSLNNGVDCFDLIDWYNHPPVVCNYCGTSGNTGMGSGSSSCEGPNN
                     CSNWNSALSVSPGETYVLNVSNFSSTQDGYTINFGASTATMVDNSPPELESLVYEPYC
                     GSSSLTIQFTEAVWCSSVQPNDFVLTGPNGTYDINSTHSIVCAAASSNTYAGTWYDDV
                     WTLELDDYLSHSGDYVLSILSGSVEDKCENVNNAVNLPFTIIGITADVNITHPISCIG
                     GGCDGEIETTNIGGGTPPYTINWSGPDGFIANTANISGLCYGEYTITITDSEGICEYI
                     ETIIMAGTPPLNPQASSNSPICQGETLDLTVTTENPAISYQWSGPNGYSSYEQNPSRP
                     ASTPSMSGRYYVGVEDEYGCQAIDSVDVIVYPVQAVTVTSNTPYCTGQDIELNATIVN
                     NATYQWVGPNSFTSTDTNPIITNCTLENAGSYSLTVTNEHSCQTSINHEIVVTPGIEV
                     DVTATDPLCYNDANGWIDITVTNGTPNYTYLWSTGATGNIAHNLIGGNQYCVTVTDAA
                     GCSLEVCRTLNNPAPIQTSVSNVATECGELDGELILNISGGVGGYTVEWTEGHTGEHI
                     TGLHPGAYTATITDANGCTKIINANVDFFGGSNASIIELQGISCNGYTTGILQANMEN
                     GASPFTYQWSVAGQTSQTLSNVGAGTYTVTVTDTYGCYGSVSYELQQPEKITISFDKT
                     DVMCRGEKTGAATVNVAGGVFPYTYQWQHGPTSSHITSLKADTYVVQVTDMNGCQHTD
                     FVVIEEPERSITIDLQVTNLTCYGKFDGSAIAEATGGTLPYNYFWRQLNSTIGSGPTI
                     HSLHAGDYSVQVFDANNCKKEVYFSISEPSEIRLYAEANHITCKGAKNGSVGVQIVGG
                     NLPYNILWNTNDTTNIVTDLGAGQYYVTVTDNNTCSKTLNILLKESSSLCLKIPDAFT
                     PNGDGINDTWQIDYIEMYKEAFVYVFNRWGQKLYQARSGDEFWDGTFNGKPVPAGTYL
                     YVIDLRNNLDPLTGTVTVVY"
     gene            complement(30347..30970)
                     /locus_tag="PKN53_03725"
     CDS             complement(30347..30970)
                     /locus_tag="PKN53_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88094.1"
                     /translation="MRTKIFICLIMFIKASACYSQQNMKFTEAKFTNEGKTVYFHISG
                     LGEDEDERQALLSDMLKDPAIISGRIFTSVENKTRCQLYLPIEIEPEYIRSFLQAKGY
                     DYDFSTISVNGEIQNTNKLQFFASPYKRASKNFEPYKSTGDKIKDAENYAIAKEQWVM
                     KNQKKYKKEIQNGTATFPIVISKEEFYNYTPEKQKRLLAEPKKYIIK"
BASE COUNT        10182 a         5069 c         4957 g        10979 t
ORIGIN      
        1 tagttttaaa tgctacaatt accgaaccta gcttattaca agccagttca agtgcaacag
       61 ctattgcatg ttatggcggt actagtacag taaccgtaag tgcttcaggc ggtacaggtt
      121 catatactgg tacaggtact ttcactgtaa gttcaggcga ttattcttat actgttacag
      181 atgccaatgg ttgtactgca actacaagtg taacaataac agatccacca caattgatag
      241 caagttctac tttcacacct gtattatgta atggaggaat ttcgacagta actgtaagtg
      301 ctacaggtgg tacaggttct tattctggaa ttggtgagca tattacatat gcaggagctt
      361 attcttatac agtaaccgat tcaaatggtt gtagttcagt aacaagtgga acagtaaccc
      421 aaccagcttt attagttgca agttcagttg caaatgatat tttatgtaat ggaggcacaa
      481 caagtgttac cgttacagct acaggcggta caggttctta tacaggcaca ggcgttatga
      541 ctaatattgg cgcaggtact catagtttta ccgttaccga tggtaataat tgttcatcag
      601 ttacaagtat aaccataagt cagccttcat tattggtagc aagctctagt gcaggcaata
      661 tattatgtaa tggaggcaca acaagtgtaa cagtctctgc tacaggcggt acaggtactt
      721 atgctggcac aggcgttata actaatattg gcgcaggtac tcatagcttt acagttactg
      781 atgcaaacaa ctgttcatca gttacaagca ttacaataag cgaaccgcct ttattggtag
      841 caagttctag tgcaggagat atattatgtt atggtggaac aactacggtt aatgtaacag
      901 ctacaggagg tacaggagct tatacaggcg taggttctat tcctaatgtt agttcaggta
      961 cacacagcta tgtcgttaca gatgcaaatg gttgtagtgc aacaacacaa ataactataa
     1021 atcagccaga tatgcttatt gtaacagcag ttgtagataa tcatgtctca tgcaatggtt
     1081 tatcagatgc acaggcaaca gcgagtacaa caggtggaac agagccttat gttttccatt
     1141 ggaatgatcc aagttctagt tatggaaata cagtacatca attgccagca ggaacttgga
     1201 cagtattagt tacagacagc aatggttgta ctgttacaaa gagtgtaact gtaacagagc
     1261 cattagtatt gtcagtaaat ataaatacag ttgatgtttt atgttttgga gaaagcacag
     1321 gtagtgccaa tgcagtagtt acaggcggta catatccatt tacatattca tggagtaatt
     1381 caggaacgac atcttctatt tccaatttaa gttcaggagt ttatgtgctt acagtaactg
     1441 acaatcaagg atgtcaggct atctctaatt cagtaatcaa tcagccacca gaattaaatg
     1501 tttatctaac ctcaagttca gcaatttgtg gttctacagg cggtagtgta cttgcttcag
     1561 ttattggagg tagtcccgat tatcaatata tttggtcagt tagtggaagt caaaacaata
     1621 ttcaaaatct aaatcctggt acttacagtg taacagttca cgattcacat atgtgtagta
     1681 aaactcaaag tatatctgta gatgttgttg gcaacatcga tgcagacata aatgtattga
     1741 gttatattag ttgttttggt cagaaagatg gagcacttca agctcttagt agcaatggta
     1801 atactccatt aaattttgag tggaatactg gagcagaaag ttcaactctt actaatcttg
     1861 gaagtggttc ttatagcgtt atcatcacag actcttgggg ttgtataggt agtgcaaatt
     1921 atgttatgat tgaacctaca gaaatagtag tagatgcaaa tattcaggga acaagttgct
     1981 ttggatatgc agatggtgca attactactc aagtttcagg cggtagttct ccatataatt
     2041 atttatggtc aaatgcacag acatcatcta acatctctaa tttagttgca ggttcttatt
     2101 cattaaccat ttatgataaa tttggttgtc aacaaataaa gacttatgct cttacacagc
     2161 cagacaaatt agaaattgat ttagatatta aagatataag ttgttttggc aatatagatg
     2221 gaggcgttaa gttgtctgct acaggcggta ctcaaccata ttcgtatgcc ttaggtattc
     2281 aagattctat ttatgcagga caaatgcatt caccattacg cacaggcagt tatgtagctt
     2341 atgttcgcga tataaattta tgtatagatt catctttatt tatgattgta agtccagcac
     2401 cattagaagc ttcttatgtt tttgaaaatc caagttgtat tggggcaaga aacggctcta
     2461 ttgaaattca agtagcaggc ggtgtagagc catatttatt tgaaactaat gatgcagttg
     2521 ttgatattcc attacttagt ggtttaagtg caggcaatta taatgtaatt attaccgatg
     2581 ctaatgattg tagccttgaa ttaaaaaata tcgttttatc tgaatctgat gttgaatgtg
     2641 ttaaaatacc taatgcattt acacctaatg gcgatggagt aaatgataca tggataatag
     2701 aaaacttaga aatgttccca ggagcaagaa tatttgttta caacagatgg ggtcaagaag
     2761 tttggattgg ttatcctggc gatgagtggg atgggaaaca caattctaag ttgatgccag
     2821 caactacata tttgtatgtt attgagttat atgatggaag taaaccttat acaggtactg
     2881 ttactatagt ttattaatat atcttgcttt aattagcaag tgtaattgtt tttttcataa
     2941 ttcctcattc aggctgttct gaaacttaga ataaagtcct gaatgaggaa ttaaaaaata
     3001 cacactttta ggacactatc atgaaaaaag taatagatac agaaaaaata cctataaaac
     3061 tttggcttga tgacattgat gaaaatacat tacaacaagc aaaaaatctg gctaatttgc
     3121 cttttgcttt caaacatatt tgtctcatgc cagatgcaca ctctggatat ggaatgccta
     3181 taggaggagt gatggctgca gatggagtaa tagtccccaa tgcggtagga gttgatatag
     3241 gttgcggaat gtgtgcagtt aagactgatt tgaaattttc gccaagtatt agaaaaaaaa
     3301 tggaaagcat tttgggagat ataagaagaa aagtacctgt aggctttaag catcataaaa
     3361 gggcacaaga agaagatctt atgcctaaag gttatggtat tgataatatg gaagtggtaa
     3421 gagctgaata taaatcagca ttaaagcaac ttggaacgct aggaggtggc aaccatttta
     3481 ttgaaattca aaaagatagt gaaaacatgg tttggattat gattcattct ggtagcagaa
     3541 atataggtaa gcaggttgca gaccattaca ataaagttgc aaaaagcctg aatcaacagt
     3601 ggaactcccc agtaccacct aaggctgatc ttgcctatct tccttttgat accggagaag
     3661 cacacagata ttttgaagaa atgaattatt gtgttgattt tgcattagcc aatcgtaaac
     3721 taatgttgat aagagtgcaa gaagtactgc ttgattattt tcctaactta aattttggag
     3781 aaacaataaa tatagctcac aattatgcaa catgggaaaa tcattttggt aaagatgtag
     3841 tagtacatcg taaaggagcc acatctgcac ataaaggcaa actcggcatt ataccaggtt
     3901 cgcaaggaac aaactcatat atcgttgaag gtttaggaaa tccagaaagt ttttcaagct
     3961 gttcgcatgg agcaggtcgc aaaatgggac gcaacgaagc agtacgaagc ttaagtctag
     4021 aagacgaaat acgcaaactt gataaaaaag gaattgtaca tgctatcaga ggcaaaagag
     4081 acctagaaga agcaccatca gcttacaaaa atattgtaca agttatggct aaccaagaag
     4141 atttagtgaa aataaaagta gaactttcac cacttgctgt tgtgaaagga taactatgat
     4201 ttgttacaaa atttatttta ttggaatatt aaattctttg gaaaaatgat tactaaaatt
     4261 attaatttta tctatttgtt ttgattttaa caagactaaa atttcgttgt tttctgaact
     4321 aaacaaacta atcttatatt tttcaaatgt tttttcattg ttttcgtttt tactttcgtt
     4381 accgcttcca ataattttaa tgtacatatc ttcttttaca ttagtccaat atccaatttt
     4441 aattaaacca aaaagaagag cagcaaactt tactctttta tttttaaagt caataaaaca
     4501 tgcattgtga gaaaatgcta aaaaagctcc tacaataata atggtaagtc caacaagcca
     4561 tgaagaaaaa ctaacataaa ttcctatggc taaaataagt attcctaaaa tcacacctat
     4621 tacaccaaaa gtaaaatcta aaatattttt tttcatattt ttactaaaga taatataatt
     4681 cgcactaagg tttaataagt aactacaact attggatttg agtactcata agtatcgttt
     4741 gctagtttta cgtaagcttt aatatgataa gctatgcttg gtgtagcact aataatatgg
     4801 ttgaaagagc ctccacccat tccaccagca gtataaactc cgtcataatt ttccgcatgt
     4861 ggagcactac taaaagacca aaaaaagcct ttttctacta tttcataagc tccatcaatt
     4921 aaaacttctg cttgcaaaga aatttcagat gcaacttcat cgtaatcagc actgtgtaaa
     4981 attactgtcg atttaccatt ttgttttata catcttattg aaaatccatg attttttcca
     5041 cttttttctt ttccaatttg tgagctatca aattttaagt gacgtgaata tgccactgcg
     5101 ttagcttcgc cagtaatttc agtcgaagtc caaatgttta aagtttctcc tctttccgaa
     5161 aacatactaa catttgttga atctctataa ccagctggtc taaaatttaa gccagcagta
     5221 tttgttgcac ctaaatttgg tgctaaccaa ttttctgtgc cagcttcttt taacatacca
     5281 ccttcatctg tacctctcca gccttcattt aaagcttcga actcttccat accaagaaat
     5341 agttctaaat aaaaccaatc gtcatcacta ggaactaccc atccactagg acatacacca
     5401 ttaccgttat aaatgttaga ttcagcagct ttaaaattat acaaatatcc gtaagtacta
     5461 tcattagata gtgttgaatt ttcatattta caataactag gtttttcttc tccatgaaca
     5521 aaagtcagcc attcttcgag agattgtacg tgttgtaaac ttatcaacat taaagtagtg
     5581 tctgttgaga cattcttttt tgtaactctc aaattttctg ccatccactc tctatcattt
     5641 attacaatag ttttgtataa atttccatcc caatctaaaa caccaggtcc ttcgacataa
     5701 agtttattta atgtttgaaa ttctattgct tcgccatagc ttgtgccatt gttattagtt
     5761 gcgtaagctc taacataata gttttgatta ctgtttaatc ctgaaattgt tgaagtgaac
     5821 tgttcactac cagcccccgc actaataaag ccaatacatg attctaatgt aatcccgctc
     5881 caagtacccc ataaaattcc tttttcactt atagggcttt caccttcatc tacaatttct
     5941 ccacttacat cagcagtaga tatacttata ttattaggag ttatagttgt aactgtaggc
     6001 gttcctatgt ttgttctaac acaacgaata gagtagccat tagtgttgct gctaggagtg
     6061 gaatattcat ctctataaat tcgttgttca gcattactta atgtacgaac aaacgttgaa
     6121 ttctctgaat taacccaagt tgagaagatt tctcctttac caacaaattc cccactttcg
     6181 gtaatataac cagaaccttg agctgaatat cctgtttggt ttgttgctcc attatttggc
     6241 gaaatccagt tgtctaattc tacttgtttc aacaatccgc cttgattagt gcctctccaa
     6301 ccagtagtat ttaacgcatc agtttcactc atgcctaaaa gcatctctaa ttttacccaa
     6361 tcattatctt ctggcacttt ccaaccagta ggacaaatat attcattctg tgcagcaaat
     6421 tgatcatata acttgccatt ttttaaatga ttatttactg aatcaaaatc catataacaa
     6481 gacttcatac taggagagct ttcccaattt tggtcaacca aaggaattaa tgtgccatca
     6541 tttagtcttg atgtgcgtaa attttcagtc atatattcag tgttggcaat tcttacagtt
     6601 ctatatacgt tgccatcaat atctgttact gtagattctg atatataata tttttctaag
     6661 gttgtaaacg aaaactcatt tgtaatactt tgtcctccag aattttgagc aaaagtttta
     6721 acataataaa ttttgtttgg ttctaaatta tttataacat acgaataatc ggattgagta
     6781 aagttagaag gtaaataatg aataccactt tctccgatag tgaaattagg atttttgtcc
     6841 caagcaactc ctaaattata taattcttct tctgattcaa catgacccga aatccttgct
     6901 gatttcatat aaactgaatc tatttgcaat atttctattg aaggctcaat tatgggttca
     6961 ggtatggttg ttgaaaaaga gatttcttcg ctataaccag tgccatgatg gtttctggca
     7021 aaagctttaa cataaaaagt ttgatttgtt gcgatattat ctagatgtac cataaattca
     7081 ttttcaaaaa tctcttgagc ataatagata tcagaattag aaaaatcaag atttggagtt
     7141 gagcttacca taaaaccacc ttcataaata gaacttactc cttgctcaat aattactaca
     7201 tgaaagtcaa cattaccatc agctgcatta aaattagctt caatagtccc taacttagga
     7261 tttccaggta tagttgagaa agtaatttct tcactataac cagtaccata actatttttt
     7321 gcaaaagctt taacataaaa agtttgatta ggttctatgc tgtctataaa aagcataatt
     7381 tcatggtcat aaatatcttg ggcataatag atgtcacagt catcatcaca atcattatca
     7441 aagtttggat tttcgcttac cataaagcca ccctcagtta tagggcttaa gccatggtta
     7501 ataatttcta caaaaaagta agcactacta gcattttcac tataataatc agcttgaaca
     7561 tttcctaaag tagggacttc ttcaactgct tttttatcgt aaacgcaacg cacagataag
     7621 cctaatgact ttgaaccatg ttctctaaat atattgcaag aggagttgtc taaagctcta
     7681 caccaagcat ttttttcatt ctgctcgctt gaagtccacc aagcagcata atcgtcttta
     7741 tagtgaaact caccagttaa agcacgcata cctcctggca tagctgtaaa accagtagag
     7801 tttacaagtt ttttatcagg caaatcccaa attccttgag ttgcagattt taaatagcaa
     7861 gcactattac tagctctcca tccaatagtg tcgatatttt gaacttctat aagattttct
     7921 ataattttcc aatcagcatc gctaggtata tgccaacctt ttggacacat tggcacttca
     7981 ttattaactg cataccaatt atataatttg ccataaattc cagaattgtt tgcattgttt
     8041 gcatatatag aatacgcagg tttgcttgtt tttcgccatt ctgcattgtc agttattgta
     8101 gttatttcat tattgttaat aaaatatttt gtgttcaaat tttctgccat ccaaattcta
     8161 ttggcaatta ctataacagg ataatgcttc ccttcaatat caatacatct gtaaaagctt
     8221 acttctaaat ctatatattc tccatcatag taatcacctt gatttatgac caaagttttt
     8281 tttgtaatat ctccttcatt tgaagtaaaa gtgaataata aaacatcgcc aaaagtatag
     8341 gctaatgtgt tttctagact ttgttttaaa taaatattgc tttcatcatt ttctgtattt
     8401 tcattttcta tatcaatact atttgcatgt cttattcctt ctatataagt ttcatcagca
     8461 gttccaatta cttttatata atatgaaaat gtgtctccgc tgatttgaat aaaataaata
     8521 ccacttaatt ttgcattaag ctctaattgg tgatatgctt ttttgttata gaaagagttt
     8581 ttataaacaa ctgcaccaga aatgttgaaa atagtaaaat ttacagttcc cgaattttcc
     8641 gcaaaataac taatgttaaa attactttct gaaggattgg gaaaaacttt aaatttttga
     8701 ggttcaagag ccataaattt taggtttgca ccactaacaa tatgaagtgt tgcgttaggc
     8761 tctatgctta atgtgttatt tgttcttaga ttttcaactt ttatactttc tatcaaaact
     8821 gtatcagagc taggagtgaa ataaatataa ttatcttgag caaataaact agatgttgtt
     8881 aaaaatagag ctaatattat ttttacagaa attaagtatg tatgtatttg atttttcatg
     8941 attgtaaatt ttaatatgcg ttaaaataat aaataaacga taaattaaaa acagagtgat
     9001 ttgcagttat tgtttcatcg tgtcttatat ttacaaaacc acgtgagtaa ttagctgata
     9061 cattataatt tttaaatcct actctaagtc cagcacatat tccagagtca aatcttttat
     9121 aagaactcca tttttctcca aaaacatcat cttcgttgtc taaatattgt gcatgtggtg
     9181 tgtcttcaaa gttccattca aaaggcggaa caatttcttc gcgaggtata aaagttcttt
     9241 ttccaactat tgcaacatta gtatatggtc ctaccatagc actaacagta acatttccaa
     9301 ttggaatttt atatcctatt tgcaaaggca cttcaaaact gccaaagtta attttagata
     9361 aagcaaaatt gtctttatat ttatctcctt ttgtaatata attcaaggaa gcaccaacat
     9421 aaagaaaact taaaatttcg tattcagcag ccaaacctac ttggtaacca aacttattag
     9481 attttttata gtcttgacct atttccgttt tttctataat tttagccatt gtagagcctg
     9541 ctgtaatgcc ataataaaaa ccttgtgcat ttagcataaa agcacaaatt ataaaacaaa
     9601 atagcaatga taaccttttc ataattttac atataacata taatagctac aaaaataatt
     9661 atttatttta taaacacagc aaatagcgtg caaaaatata tgattttata aaaaaatatt
     9721 tagacttatt cttttaagag aagtgtaaca aaaaaatatg tgtaatttcg tattttgttg
     9781 tatctttgcg ttcaatttag aaagttagaa tcaatgaaac atattcgaaa tttttgtatt
     9841 attgctcata tagaccacgg gaaaagtact ttgtctgata gactattaca atatacagat
     9901 actgtagcag agcgtgattt tcagaatcag ctattagaca atatggactt ggagagggag
     9961 agaggaatta ctataaaaag ccacgctatt cagatggaat atgaacatga gggcgaaaat
    10021 tatattttaa atttaattga tactccaggt catgttgact tttcatatga agtaagtcgt
    10081 tctattgctg catgtgaagg ggcactactg attgtagatg caacacaagg aatacaagct
    10141 caaacaattt ctgttttgta tcaggcaata gaaaatgatt tggaaataat tcctgtttta
    10201 aataaaatgg acttagaggc tgctatgcct gaagaagtcg aaggccaaat aattgattta
    10261 attggttgtg ctagagaaga tattatacat gcaagtggaa aaactggtta tggagttgat
    10321 aaaatattag atgctgttat taataaagtt ccagctccag aaggcgatga ccaagctcct
    10381 ttgcaagcat taatttttga ttctgttttt aattcataca gaggaattat tgcatatttt
    10441 agaattttta atggaaaaat ttctaccaaa gacttagtta aatttgtaaa tactggaaaa
    10501 gaatatgatg cagatgaaat aggtgtctta aaacttggtt tatctcctcg tgaaactctg
    10561 tctgctggcg atgtgggcta cattatttca ggaataaaaa ctgctgccga agtaaaagtt
    10621 ggagatacta ttactcatat cgaacgtcct tgcgaaaaag ctattgaagg tttttcagaa
    10681 gtaaaaccta tgttgtttgc tggtatttat cctgttgatg ctgatgatta tgaagaactt
    10741 agagcttcta tagaaaaact tcagttaaat gatgcttcac ttacatttac attagagtcg
    10801 tcggcagctt taggttttgg atttagatgt ggcttccttg gtttgcttca tatggaaatt
    10861 gtgcaagaaa gacttgatag agagtttaat atggacgtaa ttacaactgt accaaacgtt
    10921 caatatttag cttacacaac aaaaggcgaa caagtagtgg tctataatcc ttctggaatg
    10981 ccaagtccaa gccatttaga ctatgttgaa gaaccttata ttaaaggtag tgtaattaca
    11041 agagctgaat atgttggtcc aataataaag ttgtgcttag ataaacgtgg aacgctttta
    11101 aatcaagtgt atcttgcaac agatagggta gagctaagtt ttgaactgcc tttgggcgaa
    11161 atagtttttg acttttacga taagttaaaa agtatttcta gaggttatgc ttctttcgat
    11221 tatcatcaat caggatataa agagtcgaaa ttagtgagat tagatatttt attaaatggc
    11281 gaaatggttg atgctctttc tactttaact catattgata atgctcaagg atttggaaga
    11341 cgtatgtgcg aaaaattaag agaattaatc cctcgtcagc aatttgatgt tgcgattcaa
    11401 gctgctatag gaagtaaaat aattgcacgc gaaaccgtaa aagctcttag aaaagacgtt
    11461 actgcaaaat gttatggtgg cgatattact cgtaaaagaa aattattaga aaaacaaaaa
    11521 gctggtaaaa agcgaatgcg acaaattggt tctgtagaag tgccacaatc cgctttctta
    11581 gctgtgctta aattagatta aaatatttta tctcatatca ataacttctg cgtcaggata
    11641 taagttgttt gaatatttta tttcagtatc tggaatagaa ggattttcta aaaacttttg
    11701 aatttcgatt aaatagctaa ctccatcttt accaacatat cttgcagaat atatttgctt
    11761 tttagtttta tcaactttta aagtgatttt agaatatttc ttatcttcaa tcacttttgg
    11821 gtacatgtct atttcaacta aaggacgatt tgcttcaaat ttatcactta tatattttat
    11881 tttaaatttg tttttgtaat cttttaatat agtttttggg ttcatcatat ctgcttggtt
    11941 ttctgaatta tcagtaatgt ttatctcttc agcatctttt agataagtcc aattagtttt
    12001 accgtcagaa tgaacaattt gtcccattat gtccaactta taatatcctg ctttgtaagc
    12061 agcacttcca gaataagaat ctcttttctt agtttgtaaa ttttcaacaa acattttaaa
    12121 atcaacttta aggcttgtgt atctgtctgc tttagcagct atttcatcaa gtaaagctgt
    12181 tgcagcagca tcgttttgag agaaactatt agcataaaat aatattatgc ttaatactaa
    12241 aattattttt ttcatcattt caaaatttta atttaaaatt atttattact taagctattc
    12301 aaatattgtt ccaaactata ttcatctgga aataaaactt gtctagcttt agaaccttcg
    12361 ttaggtccta ctatatttgc tgcttctaat tggtccataa ttctgccagc tctattatat
    12421 cctatactca ttcttcgttg aataaacgat gtagagcctt gttgatgtct aacaattagt
    12481 cgtgcagctt cttcaaaaaa ttcatctctt tcgtctgggt ttatcgcacc gacacctgat
    12541 gaaccttctg ctatttctgg ttctggaagt tcaaaaacat ttggataaga ttgttgtttg
    12601 cttatatggt caacaactaa ctctatttct ggagtatcaa caaatgcaca ttgtattctt
    12661 gtgagtttac ctgtacctga aattaacata tctcctcgac ctattaattg attagcacct
    12721 gttgaatcta aaattgtttt tgaatcgtgt ccattagtta ctttaaaagc tatacgtgtt
    12781 gggaagttcg cttttataac tcctgtgatt acatctgtag aaggacgttg tgttgctaca
    12841 attaagtgaa tgccaatagc tcttgctttt gccgcaagtc gtgaaatagg agcttcaact
    12901 tgtttgcctt cttgaactat aatgtcggca aactcatcaa taacaacaac tatatatggc
    12961 atgaagaaat gtcctttatt aggatttaat tttcttgcaa taaatttttc gttgtattca
    13021 atgatatttc taactcctgc ttgtgttaaa agagtataac gcctatccat ttctatacac
    13081 agcgagttca ttgctggaac aactttacta acatctgtaa taattggatt ttcttcacca
    13141 ggaagtttag caagataatg tttttctatt cttctataaa cagaaagttc aaccattttt
    13201 gggtcaacta aaacaaattt taactgcgat ggatgtttct tgtaaagtaa agaagtgatt
    13261 atagcattta tcccaacaga tttaccttgt cctgtagcac cagcaattaa aaggtgaggc
    13321 gtttttgcaa gattgaaaac aaaggtttcg ttagaaatag tttttcctat tgctacaggt
    13381 agttcatagt ttgactcttg aaattttaca gatttaatca cagaacgcat agacacaatt
    13441 tctggttttg agttaggaac ttcaatacct attgtgcctt tacctggaat aggagctatg
    13501 attcttattc caagagcagc caaactaagc gcaatatcat cttctaaatt tttaattttt
    13561 gaaattctaa ctcccggtgc aggaactatc tcatataaag ttactgtagg tcctattgta
    13621 gcagtgattc tcgatatttc tatcttgtaa tcttgtaaag tgctaacaat tctattttta
    13681 tttgcattta attcgtctct agtaacatca gatttgcctg taggatagtc ttttaataat
    13741 tcaataggag gcattttgta atattccaaa tctcttgttg gatcatagtc tccaataaaa
    13801 tcactattga tttcgtcatc gcttagagct tcttcttcgg tttgctcgtt tattgctaac
    13861 tgtaaatttt cttctgttac aacttcttca ggtttggctt cttctttttc attttcatca
    13921 tcatcttttt catcttcatt atcaaaatct actacatttg gtatcatttc agaattttca
    13981 tcggtttttt cagtttcatc atcttcgtca ttttcttttc ctatttcttc caaatcttgt
    14041 ttgaagttat cactgttatc ttcaagcaaa ccatcagact taattgtttt tatatcattt
    14101 atttttattt tgaaccagtt taaagaattt ttaaatgtgt aaatagttat aattattgct
    14161 gtaataatta atatagtgta agttcctatt tctcctacta atgcgttgag ccacaaataa
    14221 ataaaatatc catgtgaacc gccaaccata aaataattat ctttaaaaat agcggcaagt
    14281 gcaacgctta gccaaattgt aaaaattaaa cttacaaaaa gaaaacgcca aacatttgtt
    14341 aattttatat ttaataatct taatccaaag actccaaaaa ttagaacaaa cgaaaaagac
    14401 ggtaatccaa accaattata aataaagtgg tttgatatta aagctccaat ttttcccatc
    14461 cagttttcta cgctaaagtc ggaatttgta aaaagtcctt gaaatccaac gcctagcatg
    14521 ctttggtcgg ctttccatgt gaataggtaa gaggtaaaag acaaacatag aagtattgaa
    14581 aaaccaagta gtaaaagacc tagacttatt ctaaattttt catctttgaa aaatctaata
    14641 atattattgt ctttcaatga cttcttttct tttttttctt ttgtattttt tgactttttc
    14701 gccatggttc aaaattaaaa aaaatcccca tgtagaggat ttttttttaa tctatttatt
    14761 taaacagggt tgttaataaa atattttact gtccaaacat tccgcctaat tcttcagggg
    14821 ttttaagttc gtaatcatca ggaataacaa atagtccacc ttttactttg ccagctttaa
    14881 gttctttagc tgtcattgtc attgtaagac cttgttgagt catagaatac tccataagaa
    14941 ttcctttaat tcctttaaaa ccatttaatt catttatttt ttcaggaacg ttaaattcgt
    15001 cagtgtaata aatttccact acatcttcgc cgttagttac ttcagctttt ttacatttgt
    15061 atccagcaat catttttgtt tcgtcaataa acttaatttc agggtctttc atatctgctt
    15121 cgcttaaagc tttgtcaatg tcttcttttt cttgcttcat agcaattttc attcccatag
    15181 cgtccataag tataattacg ccacctttgt caaagtcaga aattgaagcc atagaataaa
    15241 aagctgatac ttggtcggtt ctcactttat ttccactaat gtaagtaatg atattggtag
    15301 gcaattgagc ctttgtcatt tcatctaatt cttcttcaga atcataagta atgtcataag
    15361 taactgttcc tctaaagggt tttgcctttt tttgagaaaa actagaaagg cttaaactaa
    15421 ctgccactaa aaccaaaagt aatttgaatg ttgttttcat agaatattaa tttttaatgt
    15481 taagcttaaa atattataca attatcatac cacaaagata taactaaatt tttgtaaata
    15541 aacatttttt tgtaaaaatt attaaaatat tgttaaggga ttggattaca atgaacagat
    15601 aatattccaa tcttacttta taacaacatt cacaatcttt ttaggaacaa taatcatttt
    15661 tacaatttgt tttccttcaa tccatctttg tgtgttttcg ttgctaagaa cagcttcttc
    15721 aatttgttta ttatccaaat caagtggcag ttctaatgtg aaacgagttt ttccattaaa
    15781 agaaattgga tattcaaaac tgttttctac tgtatatgca ggattatatg tggtccacat
    15841 agcttttgta atgctttctt tatgaccaag caaattccac aattcttcgg taatatgtgg
    15901 tgcataaggc gacattaaaa tacaaagtgg ttctaaaatt tctctcttat tgcattttcc
    15961 taaatcgtta atgcaaatca ttaaagcact tacgccagtg ttaaatgcaa atttttctat
    16021 atcatcactt agttttttaa ttgtgttatg aagaattttt aattctgctg catttgcttt
    16081 gtcatcactt acaaaaaatt catcattatc attgtggaat aatctccaaa acttacgaat
    16141 aaatctaaat actccttcaa ttccatttgt gtcccaaggt tttgcttgtt ctataggtcc
    16201 taaaaacatt tcatacattc taagtgtgtc ggctccatat ttttcaacaa gctcatcagg
    16261 attttgaaca ttgtgcatag atttcgacat tttttctatt tcccaagaac aatgatattt
    16321 gccatctgct tctaaaataa attctgcatc tttaaattct ggttgccatt ttttaaaact
    16381 ttcaatatct aaaatgtcgt tgtgcacaat gttaatgtca acatgcaatc tttgagtttc
    16441 gtattggtct tttaaattat aagaaacaaa tttatttgtg ccatttattc tgtaaacaaa
    16501 gtttgaacgt ccttgtatca ttccttggtt tatcagcttt ttaaatggtt ctttgtttgg
    16561 cactaatcca agatcgtata aaaacatatt ccaaaaacga gaatataata aatgcccagt
    16621 agcgtgttct ctaccgccaa tgtataaatc aacattttgc caatagttaa cagcttcttc
    16681 cgacactaat tcattgtcat tatttgggtc catatatctt aaataataag ctgatgaacc
    16741 tgcaaatcca ggcatagttg atgtttcaag tttgtgccca tcggcggttt gccaattttt
    16801 agctcttgct aatggtggtt ctccatcgtc tgttggtaaa tatttatcaa tatcgggtaa
    16861 ctcaagcgga agctcgtcta tgctcattgt tttaggaata ccgtttttat aataaattgg
    16921 aaacggttct ccccaatatc tttgacgact aaaaattgca tctctaagtc tataattaat
    16981 ttttactttt ccaagaccta gttctgaaat ttttgaattc attttactaa tagcttcttt
    17041 aacttctaag ccatttataa aatctgaatt aattaatatt ccgtcttttg agtcgtatga
    17101 ttctgtccat tcgcttgttg gaataatttt ttcgtttgga ttttttatta cttgagtaat
    17161 attaagatta aaatgtttag caaaagcaaa atctcgtgag tcgtgagcag gaaccgccat
    17221 aatagcacct gtaccatatc caattaacac ataatcagca atataaattg gaatttcttg
    17281 ttttgtaaat ggatttatag cataagaacc tgtaaattga ccagacactt ttttctctgt
    17341 cattctttca acttcagaac gattttttgc ccaatttata tattcttcaa cttttacttt
    17401 gttttcagca cttgttaatt cagagcaaag ttcgtgttct ggagctaaaa ccataaagct
    17461 aactccaaaa attgtgtcgg gacgagtagt aaaaatttca atttttttat ccgaatcttt
    17521 aatgtcgaaa aacatttgaa caccttcgct tcgtccaatc cagttggttt gaatatcttt
    17581 aacaggttcg ggccaatcaa tagtttgtaa accttctagc aatctgtcgg cataagcagt
    17641 aattcttagc gaccattgtt tcattttttt cttttcaaca ggaaaaccac ctctaacaga
    17701 aagaccatca gcaacttcat cgtttgctaa tactgttttt aattttggac accaattcac
    17761 tgttgtgtcg gcaagaaata gaagtctata tttcattaaa acttcttctt tctcttcttt
    17821 cgacattgta ttccattgtt cagcagtaaa atcttcatgc tcgtcgcaat atgcacttac
    17881 cgctttagaa ccttgtgttg caaatatttc tgtcaaatgt tttatagaat ttgctttatt
    17941 aagtttagtg tcatagtaat gttcaaatag ctgaactaca gtccattgag tccatttgta
    18001 gtattttggg tcgctagttc taacttctct atcccaatcg tatgaaaaac caagattgtc
    18061 tagttgttcg cgatagcgtt ttatgttttt ttctgtagta attgcgggat gcgtacctgt
    18121 ttgaatagca tattgctcgg ctggtaagcc aaaagagtcg tatcccattg gatgcaaaac
    18181 attgtatcct tttaattttt tatatctgga ataaatatct gaagctatgt atcctaaagg
    18241 atgtccaaca tgcaagcccg aaccagaagg ataaggaaac atgtcgagca catagtattt
    18301 aggtttttta ttgtcaattt caactttata agttttgttt tctttccagt aagcttgcca
    18361 ctttttttct atttctttga aattatattc cataatcaat aaaattttgt gcgaaattaa
    18421 aactttttat gtataaaagc ttctaaatat tcaaaaaaac caattagaat aagtttaagt
    18481 tctaattatt atttataact ttgcagtaaa ttgtaataaa tatgaagaaa tccgtaattt
    18541 taatttcgct tttatcaata gtattatatt cttgtaattc aggttcttat aaaactgtag
    18601 cttatgctca tggacaagct ggcgatatta ttgtagtaat gaataatcaa gcttgggaca
    18661 cagaagcagg cgatagtttg agaaaagtat ttcatgaata tgtttcaggt gtgccaatgg
    18721 aagaacatat gtttgattta catcaaatac ctagagaaca atttgtaaaa ggaaataaac
    18781 ttcatagaaa tgtaatttat caagaagtaa actcttctat tgagcaagca agtattaaga
    18841 tttcaagaaa taagtttgca atgaaacagg tttttgtaga aattgcagca cccaaccaac
    18901 atgaatttgc taaaatagtt gcacaaaata agaaaaaact tgttgatata tttctagaag
    18961 cagataaggc aagatggata gagactttaa caaaatatac caacaagatt gcaagtaaaa
    19021 gagtacaaga aagatttgat atttttctac gtattccttc atattataat attgaagaag
    19081 acagagatgg atttatgtgg atatgtaagg aagcaaaaaa ttatagtact aatattttaa
    19141 tttattcttg ggaaaaatct gatacaagtg ttttaacagc cgaatattta atttctatga
    19201 gaaatttaat actgcaaaac aatattcccg gagaaaaacc taattcttat atgacaacag
    19261 aaacaaaata tgactatcct gaattttctt tacttatgca caattcaatg caaacaggca
    19321 aactaaaagg cttgtggcga gtgcatggcg attttatggg tggtcctttt gtaagttata
    19381 caaaaattga tgaaccaaga aatagattag taacagtaga aggctttgtt tatagaccta
    19441 atcaagaggt gagagacctt attagagagt tagagtggac ttgttcaact tttgatataa
    19501 ctttttaatt tatcatattt tgtaaaaatg taaaataata atatttttgt aaaaaattaa
    19561 ataagaaaaa tatgaatttg aataaagtga tgttaatcgg tcgtttgggc aaaaagcctg
    19621 aagtaaatta tattcaacca gatttaccag tagcaaaatt ttctttagct acaagcgaat
    19681 attataaaga caaaacagga gctaaaatag aaaatacaga gtggcacaat attgttgctt
    19741 ggcgtgggaa tgcaaagttt gccgaaaatt atttaaacaa aggcatgcta gtttatattg
    19801 aaggcaaaat tcaaactaga agatgggaat ctcaagatgg aactgcacgt tatgtaacag
    19861 aaattgttgc tgatattctt agaattttag aaaaacgcga aaatagagaa tttactcaaa
    19921 attctcaaac tcaaaataca gaagcaaatt ctaaaagcag ctttgatgaa agctcaaaac
    19981 aagaaagcca agactttatg gctgatgatg atgtttttgg tggtgatgat ttaggaaata
    20041 atgatatctt acccttttaa ttaacataat tatacaactt tggaccctga accgtttata
    20101 aattctatta ttttacttaa tgcagctttt gatacaaaag ttgttataat agaagcaatt
    20161 gcaatagtgg ttttattatt ttgctcagga atgatttctg ctagcgaagt agcttttttt
    20221 agtttatcag caaaagattt acataaatta aagaaaactt caccaaaatc gtttgagaga
    20281 atagttagac aattagaaag accaaattgg ttgctgtcaa caattcttat tgcaaataat
    20341 tttgtaaata ttacaataat tattctttca acctatttaa cccaatacct atttgctgga
    20401 atggatacaa atagtgttgc ttatatttta ttgcaattag tattaataac atttttaatt
    20461 ttattgtttg gtgaaataat gccaaaaatt ttagcagcaa aaattcctgt taaagcagct
    20521 aaagctatga gcttgccttt gttgatttta agcattatat tttatccaat aagttatctt
    20581 ttaattcatt caactaattt tattaataaa agattagaaa aaaaacataa aaataaaatt
    20641 agcattgaag aactttctca cgttattgag ttggcaggcg aggatctgaa agaagataaa
    20701 gaaatgcttg agggaattgt taattctatt aatttaagtg ctaaagaaat tatgacatca
    20761 cgtattgatg tttttgcatt ggattataat ttaacttttt ctaaagttgt tgagggaata
    20821 gtaaattctg gattttcgag aataccagtt tatgtcgata atttagataa tattaaaggc
    20881 gtgctttaca tcaaagacgt gttgccttat atatatttgc aaaataaaga tgattttaaa
    20941 tggcaaaaac ttattaagcc gcattttata gttccagaat caaaaaatat taatgaatta
    21001 ttgaaaaatt ttcaagataa aaaacttcat atagcaatag tagttgacga atatggaggt
    21061 gtgagtggtt tggtaacttt agaagatgtg cttgaagaat ttgtcggaga aataaatgac
    21121 gaatcagacg aagatgagga aactttattt gaaaaaatag atgataaagc ttatgtgttt
    21181 gatgctaaaa ttagtttggt tgacttttgt aaaattattg atgttgatta ttccatattt
    21241 aatgaaatta aaggagaatc agatagcttg gcaggcttga ttttagaggt aaatggcgaa
    21301 atccctaaac aaaataaagt tttagaaatt gcaggatttt tatttactat cattgattct
    21361 gatgaaagaa aaattattaa aataaaaact aaactacctg atgaagttca ataaaattca
    21421 tattcttttt tttcttatag cttttatttt tatttcatgt aaagataaaa atactattcc
    21481 taaacctaga gcatatttta gaatagattt tcctgaaaaa gcttataaac agtatgatga
    21541 cgattgccct tttaagtttg aaattcctgt ttattcattt ttaattaaaa ataaggaaga
    21601 aaattgctgg atggatatat attttcccaa aaatagagca actgtttata tgacctacaa
    21661 ggaattagac gataatttac gtcaatatct tgaggatcag cgtgaatttg tttacaagca
    21721 tgttgttaaa gccgatgcaa ttgaagaaac tgagtttata aactatgatg ataaggttta
    21781 tggtatgctc tataatatta aaggcaatac cgcctcttcg gttcagtttt atcttacaga
    21841 tagtgttaat cattttttgc gtggctcctt gtattttgat gtaaaaccta ataaagactc
    21901 attggctcca gttatcaatt ttattagagg tgatatagag cattttatgg aaagttttag
    21961 ttggaaataa aatgtgtgtg ttaaaaaatt ttgttcaagg taaagctatt tctggcataa
    22021 aagaaatttc aaaagattta aactatttgt attcttattg cgacctgaca gctgttgaca
    22081 atatgctttt aatgcagtct ggttctttgg caaggcgttt agaaatttta aatactcgag
    22141 ctttaataaa agattgtggt ttaaaattaa gcataattta taaaaaccga aagccaatag
    22201 tagaaaatgg ttttgtaagt atttcgcatt cagatagttt ggttgctttt gtctggaatc
    22261 ccgattttga aatttcaatt gatattgaag aaataggcga aagaattaca cgaatcgcaa
    22321 gaagagcttt ttcagaagat gaattaaaat ttgcaaacga tgatatagta aagttaaatt
    22381 tgttgtggaa tgtaaaagaa tgtgtttaca aactagcagg tataaaaggt ttggaattta
    22441 aaacacaaat ccatgttttt ccatttgaaa acattgaaaa catcgaagca gagctaatta
    22501 cttcagaagg taaacgaaat tttttatttc atggcaaaat tttaaaagag catagcttgg
    22561 tgtatggtag tgaaaaagtt aaaacattta acagaattta atcaaaaatc ctcttttaac
    22621 tttatacttt taacttttaa ctgattatgc gtcagcccct ttaccttcgg tgagctcgta
    22681 aactcggttc aactttcaac acttcaacta cgttcagtgc atcgctttta acttttaact
    22741 gaattaagat atatattctc tccatttgtt tatgcaattt tgcatatctt cgggcaggtc
    22801 ggaatcgaaa caaacttctt ttcctgttga gggatggata aaacctaatg tttttgcatg
    22861 tagggcttgg cgtgggcata ttttaaagca attctctaca aattgtttat atttgttaaa
    22921 agttgttcct tttaagattt tgtctccacc atattcccag tcgttgaaca aagggtgtcc
    22981 aatgctttta aaatgcactc taatttgatg agttcgccca gtttcgagtt ggcattcaac
    23041 taagtttaca taattaaatc tttctaaaac cttataatga gtaattgctt ctttgccgta
    23101 ttcaccatct gggaaaacat ccattatttt tctatttttc aaatttcgtc ccacatgtcc
    23161 ggtaatggtt ccttcatcat ttgctaaatt tccccaaacc aaagcttggt attttcgttt
    23221 tgttgtatgg tcaaaaaatt gtttagcaat tttattcatg gcaatttcat tttttgcaac
    23281 cacaagcaaa cccgatgtgt ctttatctat tctatgcaaa agtccgggac gaatttcatt
    23341 tccaggagtt gaaggtaatt gttgaaattt gaacattaga gcattcataa gcgtgccttc
    23401 ataatgtcca aaactaggat gcacaaccat tccgggattt ttgtttacaa ttacaaaatc
    23461 atcatcttca tatactatat taataggtat atcttgagct attagttcag tttcggtttt
    23521 aggaaaagaa agaactatgc tgataatatc gccgggtttt actttgtaag aatttttact
    23581 tggtttgccg tttactaaaa tatttccagc ttcggcagca ttttgaatac gatttcgcga
    23641 tgtggaagcc aatctgcaaa cgagatattt gtccaaacgc attaattttt gtcctggctc
    23701 aataactaac ctgtgatgct caaaaagctc ttcggactgt tcaaattctt cttcattaac
    23761 ttgtggttcg agatgttctt tgtccatttt taacgggcta tgagcaattt ttgtgttttg
    23821 taatttgcta agttagaatt taattttact acatacattc ctgaacttaa agcagagatg
    23881 tcaatttgac tttttacttt agaagataat attaattttc cagtaaaatc gtaaatttga
    23941 actatgtcgt tatcagagaa agattcaata tttataaatt catttgcagg attaggatat
    24001 atttgaaact caacaaaatt atctacaagc acattggatt gttgtttttt aatttcgtat
    24061 tcaaaaactg gtcttaccat caaagcacca aaaattgaag agttttgcca agttccatca
    24121 acattataaa acaagtgttg gcttgcatca ttatgaatat cgaaaccgca attaagcata
    24181 tcgtcagtag tttttgtcca accaatataa aaaacatcat taatatttaa gactgtatct
    24241 aagggataat aaacaaaacc atttatttcg tcgctatatt ctggcagtac acctagtttt
    24301 tggaaaattg tatctcccgg aacgccacca ttatcttccc atacatttaa aaagaagtat
    24361 tttttatttc cttcgtttaa aactttatta aaataaatat atactccgta taatcttcca
    24421 tttgtgattg gtttaaattg atatgctaag cttgcgtatt tggtattttg tcctgatatt
    24481 ccatatcctt tttcagcagt accatcatca taagcataat agttttcaaa tttttgataa
    24541 tatctgatag tatcattcca gcgatatcta aatctttcgg cagcgtcatc agttgtgata
    24601 tagcctttaa gtaaaaatct agcagagtct tcagagttgg aattgaaaac atatggaatt
    24661 cttctgctat agcttattgt ttcaaaggct tgtatatttt cgttatcgtc attcattgaa
    24721 aaacctgaat tatttccata taaatcaata atttcaattt gtctattaat agcaaaaata
    24781 tctggtcctg tatttctata aacccatgtt agtgtgtcgt ttattaagcc agaatgattt
    24841 gctttatagt gtttccatgg aactgcttca aatccgttaa gcatagagcc aaaattttta
    24901 ataaaggcaa cgtcatttgc tattgtgtct ttatatgttc ttgcagtatc aagcattaca
    24961 aagtctatgt tccaatggtc gcaattgcta atccaactag gttcgtagtt tgatgatatg
    25021 cttgcataat ttaaaaatct aaattgaaaa ccttttacga gtaggagggt gtctgttatt
    25081 ggaattaaaa catttttaaa ttcactcatt gcgtttccgc ctgcaacatg ccatacactt
    25141 ctccatggta agtcaggagt tttaaattgc acaacaagag aatctcctgc ttctggtttg
    25201 ttcccatttc caccagcttg atagaaaaag ctaaaataca cagagtcact tggacttaca
    25261 gcaagattaa tcggttttga agttaaatag tcagctattg cagaacttag agaaggtaag
    25321 tgagtgtata tattaccaaa ttcgtcagca gcatcaagag ttgctgtcat ataggtcatt
    25381 gggtttatgg cataagtatt atttatgaat gcatatttat ccaaccattt tgttgaatcg
    25441 ggataaacat aagaagctga aaaatcatca aaaaaaggaa gttccaaagt gtcaacaaca
    25501 gggtcatttt ttagacttat tttattgaag tagttgcgac tatattgttg caaaaacata
    25561 ttgccatagt caacagtata ttcttgagca atagaaatta cagaaattag aattgaaata
    25621 tataaaaaac ttaatttttt catcattaat ttttattcat cagtattttc attttcttct
    25681 tcacttagct tagcatttgt ttcatctata gcttcgtcaa ttacagattg gtcttttgtt
    25741 aaccaaatat caacactata acctaaattt acttgattta ttgtgctagc tctaggcgat
    25801 tgtcgataaa tttttgcatt tgttgaatct tgcgaacttc taacagtatt atcataaaca
    25861 acagccccaa tgttaagata taaatctaat aaaagattag atgcctcttt tttagttaag
    25921 ccaattaaag tagggacatt tgttgttttg tcgctaagtc ctaaaccaag aactaaatca
    25981 attgttgcac ctttttttat ttttatgccg ggttctatgt ctcgtcctct gtataattgt
    26041 ctaattacaa catttacaga aatgtcagga acatacttta aatttccgac ttttagtcca
    26101 taattttcag ccattgagtt taccattttt actgacattc caataaattg aggcatagat
    26161 atttgttcag tttcttgcga gtttattttt acataaattt ttcgtccact ctttacaaga
    26221 gaaccagctt ctggattttg ttctgttatt gcaccttttg gaaatttatc atcaaaaaca
    26281 gtatcaacaa taacatattg aaacccattt tcttcaatgc ttgctaaagc tttgtcggct
    26341 tgttcgccaa caaactctgg tactgtaact gatttaccat gttttgtgta tatttttaaa
    26401 gaaaaattta ctattattac aataataata acaagagcga tagcagccag cacattaaaa
    26461 ataaataatt tgcttttcaa aaaatttaaa aatttatttt tgcctttagc ttttttcata
    26521 caaaagattt taggttgtaa aaatattaaa aatctatgaa tacacaatga aaatataaga
    26581 tagatttttt ctaattttat ttttttgtaa taaaactagc attaaagcag gtggttttgt
    26641 tagaattatt tttaataaac tacagttaca gttcctgtta gaggatctaa attgttgcgc
    26701 agatcgatta catacaaata agtccctgct ggtacgggtt tgccattaaa tgttccatcc
    26761 caaaattcgt caccagatct agcttgatat agtttttgtc cccatctgtt aaatacataa
    26821 acaaaagcct ctttatacat ttcgatatag tcaatttgcc aagtatcatt tataccatct
    26881 ccatttggtg taaatgcatc tggaattttt aagcataagc tagagctttc tttaagcaaa
    26941 atatttaaag ttttactaca agtgttgttg tcagtaacag taacataata ctgcccagct
    27001 cctaaatcag ttacaatatt tgttgtgtca ttagtattcc ataaaatatt atatggtaaa
    27061 tttccaccta caatttgtac gccaaccgag ccatttttag ctcctttaca tgttatatga
    27121 tttgcttctg catataatct aatttcacta ggctcactaa ttgaaaaata gacttctttt
    27181 ttgcaattat tagcatcaaa aacttgcaca gaataatctc ctgcatgtaa tgagtgaatt
    27241 gtaggtccac tacctatagt tgaattaagc tgacgccaaa aataattata tggtaatgtg
    27301 cctcctgtag cttcggcaat tgcacttccg tcaaatttgc cataacaagt tagatttgta
    27361 acttgtaaat caatagtaat agatctttca ggttcttcaa tcacaacaaa atcagtatgt
    27421 tgacatccat tcatatctgt aacttgcact acatatgtat cggctttcaa tgatgtaata
    27481 tggctagatg taggtccgtg ttgccactga taagtatatg ggaatactcc tccagcaaca
    27541 tttacagttg ccgcacctgt tttttcgcca cgacacatta catctgtttt atcgaaagat
    27601 attgttattt tctctggttg ttgtaattcg taagaaactg aaccatagca accatatgta
    27661 tcggtaacag ttacagtata agtgcctgct ccaacattgc ttaaagtttg ggacgtttgt
    27721 cctgcaaccg accattgata agtgaatgga ctagctccgt tctccatatt ggcttgtaaa
    27781 atacccgttg tgtatccatt acaacttatg ccttgtagtt ctataattga tgcgttagaa
    27841 ccaccaaaaa aatcaacatt agcgttaatt atttttgtgc agccattagc atctgtaata
    27901 gttgcagtat aagctccggg atgaagtccc gtgatgtgtt ctccagtatg tccttctgtc
    27961 cattctacag tatatcctcc aactccgccc gaaatattta atattaattc tccatcgagt
    28021 tctccacatt cagttgccac attactaaca cttgtttgta ttggggcagg attatttaaa
    28081 gttctacaaa cttctaggct acagccagca gcgtcagtaa cagtaacgca atattggttt
    28141 ccaccaatta aattatgtgc aatattacca gttgcaccag tactccacaa ataagtgtag
    28201 ttaggtgttc cattagtaac agttatgtct atccagccat ttgcatcgtt ataacacaga
    28261 gggtcagtag cagtaacgtc aacttcaata cctggcgtaa caactatttc atgattaata
    28321 gatgtttggc aactatgttc gttagttaca gttagagaat aagatccagc attttctaaa
    28381 gtacagtttg ttataattgg atttgtatct gtagatgtaa atgaatttgg tcctacccat
    28441 tgataagttg cattatttac aattgtagca tttagctcta tatcttgacc agtacaatat
    28501 ggggtgttgc ttgtaactgt aactgcttgc acaggataaa caataacatc aacagagtct
    28561 attgcttgac aaccgtattc atcttcaaca ccaacataat atcttccact catcgaaggt
    28621 gttgaagcag gtctgctagg attttgctca taagaactat agccgttagg tcctgaccat
    28681 tggtatgaaa ttgcaggatt ttcggtagtt acagtcaagt caagggtctc gccttgacaa
    28741 ataggactat tgcttgaagc ctgtggattt aatggaggag tgcctgccat gataatggtt
    28801 tcaatatatt cacaaattcc ttctgaatct gtaattgtta tagtatattc accgtaacac
    28861 aatccagaaa tgtttgcagt attagcaata aatccatctg ggccactcca atttattgta
    28921 tatggaggag tgccaccacc aatgtttgta gtttcaattt ctccatcaca acctccgcct
    28981 atacagctaa taggatgtgt aatatttaca tcagctgtaa tgccgattat agtaaatggt
    29041 aaattaacag cgttgttaac attttcacat ttatcttcta cacttcctga taaaatagaa
    29101 agtacgtaat ctccactatg agataaatag tcatcaagtt ctaaagtcca tacatcatca
    29161 taccaagtac cagcataggt atttgaagaa gcagcggcgc aaactatact atgagtacta
    29221 ttaatgtcgt aagttccatt agggcctgtt aaaacaaaat cgttgggctg aacactagaa
    29281 caccaaacag cttctgtaaa ctgtatagtt aaagatgagc taccgcaata aggttcatat
    29341 actaaacttt ctagctctgg gggagaatta tccaccattg ttgctgtgct tgcaccaaaa
    29401 tttattgtat aaccatcttg tgtagagcta aaattagata catttaaaac ataggtttcc
    29461 ccaggtgaaa cgcttaaggc agagttccaa ttagaacaat tattaggtcc ttcacaacta
    29521 gatgatccgc ttcccattcc tgtattgcca gatgttccgc aataattaca aactacagga
    29581 gggtgattgt accagtcaat taaatcaaaa caatctactc cattattcaa actataaaca
    29641 gcccaatcgt aatcatcact actattataa ggagttattg taaaagctaa ttcgccacta
    29701 gtttgagcag taaaagtgta ccacacattg tttttttcgc ctgtaactaa acaattagga
    29761 caattatgcg tagaaacagc cattccttgg tgttcgttaa agttaaataa atcataatag
    29821 tgtcctgtac ctacataaga ttgtggatga ttagagacac ttgcgcataa tggtctagca
    29881 cctaaacaat cttgttcggt tggtgtgggt agttttttga aagcaacggt tcctaatgct
    29941 gttccatcag ttgagcaata aaagttatat aatccaatag aatatgttcc acttgtaggg
    30001 caaacccaaa ctaattccga accataacca caatggtcgt cgtttgattg ataagttaga
    30061 gagccgtcag cattatgtaa atctatcatt gggtcgtttg tgtaagaacc tccaccttgg
    30121 caaaatgaaa aaataataac ttcgccagct gcaagtgtaa aagtatatct atctcctgct
    30181 tgaactcctg atattgtttg ccaattatct gttggggtta aatttcctgc tagagtactt
    30241 ccagaacaag caaaactata attaaataaa gctgtcgaaa atatcagaca tgctgacaag
    30301 ataattttat gtaatttttt gttcataact accaattttg acattattac ttaattatat
    30361 atttcttggg ttctgccaat aagcgttttt gcttttctgg cgtataattg taaaactctt
    30421 ctttagaaat aacaatagga aatgttgctg ttccgttttg tatttctttt ttatactttt
    30481 tttgattttt cattacccat tgttctttgg caatagcata attttctgca tcttttattt
    30541 tatcgcctgt ggatttatag ggctcaaaat ttttagaagc tcttttgtat ggagatgcaa
    30601 aaaattgtaa tttatttgtg ttttgtattt ctccatttac tgaaattgta gaaaaatcat
    30661 aatcatatcc tttggcttgc aagaaacttc taatatactc tggctctatc tctattggta
    30721 aataaagttg acaacgagtt ttgttttcga cacttgtgaa aatacgtccg cttataattg
    30781 caggatcttt taacatatca cttaatagag cttgtctctc atcttcatct tcgcctaaac
    30841 cactaatatg aaaatataca gttttacctt catttgtaaa ctttgcttca gtaaatttca
    30901 tgttttgttg agaataacaa gcagacgctt ttatgaacat aattaaacat ataaatattt
    30961 ttgttctcat attttagaaa ttattaataa ataatttaaa tggttaattt aatctcctac
    31021 acaaagccca ttataattaa cttaatattt taacggattt tttacaaaaa ggttgctaat
    31081 ttgtaactaa attaataaaa tcgttattaa atagttgttc ttctaaattg ctgtcctgtt
    31141 ttccgcattg ggacacccaa aaaaataatt cattaataat tttataa
//
LOCUS       DAOPNX010000021        29767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_2001_length_29767_cov_6.992697, whole genome shotgun sequence.
ACCESSION   DAOPNX010000021 DAOPNX010000000
VERSION     DAOPNX010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 29767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29767
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2001_length_29767_cov_6.992697"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..397)
                     /locus_tag="PKN53_03730"
     CDS             complement(212..397)
                     /locus_tag="PKN53_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88095.1"
                     /translation="MNKKTKKYEVKIHYSGFCTYEILAENESEAILKARNLQINSKEL
                     LSNIDNWEEADIASEVQ"
     gene            complement(450..1085)
                     /locus_tag="PKN53_03735"
                     /pseudo
     CDS             complement(450..1085)
                     /locus_tag="PKN53_03735"
                     /inference="COORDINATES: protein motif:HMM:NF022902.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1206..1994)
                     /locus_tag="PKN53_03740"
     CDS             complement(1206..1994)
                     /locus_tag="PKN53_03740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOD88096.1"
                     /translation="MELEKTYNENCLITMQNMPDNYVDFVITSPPYDDIRNYNGYQFE
                     FEKIAKELFRITKQGGIIIWVVADATINGSETGTSFKQALFFIEIGFRLHDTMIYYKN
                     NPMPQTGNRYHQHFEYMFAFSKGSPKTFNPITEPTKYQGLANMKNRGQNGSLDYEKVE
                     RTKEKKVGNVFFYSVGGGISTKDKIAYNHPAIFPEKLVADQIKTWTNENDLVYDPFMG
                     SGTTAKIAHLLKRRWIGSEISEEYVKISEERLKEYTINNLFTSI"
     gene            complement(2079..2687)
                     /locus_tag="PKN53_03745"
     CDS             complement(2079..2687)
                     /locus_tag="PKN53_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOD88097.1"
                     /translation="MRTKVLVIFLIYLGFSMNMNAQEIKLPAPDKKGGKTLMETLNER
                     KSTRSFTDKELSNQQLSNLLWAANGFNRDDKRTVPTARNCQELELYVILKDGIYFYNA
                     KEHKLTLTIKGDYRKNAGLQEFVADAAVNILIVADTEKAASSHYSYTNCGYISQNIYL
                     YCASEGLGTVARGAFDKEELHKILQLPENQEILLAQTVGFAK"
     gene            complement(2811..4367)
                     /locus_tag="PKN53_03750"
     CDS             complement(2811..4367)
                     /locus_tag="PKN53_03750"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlwI family type II restriction endonuclease"
                     /protein_id="HOD88098.1"
                     /translation="MTNKKNKRQATYKQMAFETAVRNPERYKEILTAILPYINKVLND
                     ETLLQVVSSLYLKGVVSSKNVIIDENSTIENIADAVISVNSTRRADGGFPEGYQSRFW
                     TYVRTLSEMGFVFAQYQQPLKFSNIAIKLINNEIDEQEAFSTQAMKYNRRSPYRNVLN
                     DFNYFKFILEILKQRERISYEQFIISTFSTNGDVGEFLQLINKNSFADLSEVETFLRS
                     KYETNLKTQTILRDYPDVILRLLIITGFVSIQFSGKVFVYRNIANDNYINDLLAINIE
                     LTETEKENPNLHFTKLETYNKQLLKIINKHREEIAKKKDIEYVQKVSKIIKMYNLNEK
                     IIVESINAIGTTKNIIPAFKYIPDPLKLEFYLSLILTLKYGKKFAINPNYKADYIGLP
                     ISHAQGNKGDIEVYSKNIYWLIEVTLIRNKTQQLNSETTTAIRHFLEDNKINNYLSKY
                     LSFVAPIIHQDTKEFYDFSIVKHKSKEQSINIKPYSIPEFIDVTLKTDNFNDMENYTN
                     QIINDFKNNY"
     gene            complement(4380..5306)
                     /locus_tag="PKN53_03755"
     CDS             complement(4380..5306)
                     /locus_tag="PKN53_03755"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009010327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HOD88099.1"
                     /translation="MKKEKINRSPLFYVGDKYKLIKEIKTHFPPSINQFIEPFVGGGS
                     VYLNVKAKKYLLNDIDKNVIDIHKFLCSFVEKENEFYDLIFSIIHKYNLSLSYEKDIV
                     PFELKKRFKKTYYAHFNKENFNRLKKDYNQSKEKDIAVLYVLLIYGFNRMLRFNNQGN
                     YNLPVGNIDFNRNVFNALSDYFKLNKTKQAYWYNLDFKTFLKSINFQKNDFIYLDPPY
                     LITLSEYNKLWNEETENELLQTLDILDKENIKFAISNITHYKEKENSLFIDWSSKYNT
                     YQIKSNYISYHDNTIKKINEVLVTNYKSNMTL"
     gene            complement(5260..6630)
                     /locus_tag="PKN53_03760"
     CDS             complement(5260..6630)
                     /locus_tag="PKN53_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOD88100.1"
                     /translation="MYTIGHLLKEIREEIGLPLQEVQQELGIDLTLLSKIENGKRLPT
                     IEQLQKLSKFYNYDDKLLIIQRESDKIANSLEYPELATETLKVAEDKIKYGNQYIQLF
                     QDTIYQKPIPIESRRYIGSKAKLTEWIMNLIDKETENINTFVDIFAGTASVSNQAIQK
                     YNQVIINDILYANNVIYKGFLKKGKWDKAKLNDIITNYNRLDADLIDENYFSENFGGK
                     FYEHNTAKLIGYIRQDIENLKPELTDKEYNILLTTLIYNIDKLANTVGHFDAYIKKPI
                     KPRPLRLRLIDAQDFDNVKIYREDANKLAQQINSDLVYIDPPYNSRQYNRFYHLYETL
                     VKWDKPKLYGVALKPAPENNSLYCTTKAKNAFKHLVLHLNTNFLVVSYNNTYNSKSHS
                     SENKIKLEEIEYILNKCGKTKIFECSHRFFNTGKTEFDNHKELLFITEVDEKRKNKSL
                     TSLLCR"
     gene            complement(6695..7378)
                     /locus_tag="PKN53_03765"
     CDS             complement(6695..7378)
                     /locus_tag="PKN53_03765"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOD88101.1"
                     /translation="MKTKQLALLTITVFLTCYSFCQPSILSLKEIEKNFIKVNDNLYA
                     YKYETSNELYNLFLSKQTGSDINLLKVDSANWRLPKYSNEPYAQYYHLHPALANYPVV
                     NISYEAAVEFCKWLTECYNNYSDRKYKKVEIRLPSSEEWNMLLSKIESKASKKQTDNN
                     VRPVDKYKNTGKFFNVLENVKEMTNTKGHAKGGDFENGINPSDFDYKYKEASLPFVGF
                     RFFIDIVEK"
     gene            7532..7879
                     /locus_tag="PKN53_03770"
     CDS             7532..7879
                     /locus_tag="PKN53_03770"
                     /inference="COORDINATES: protein motif:HMM:NF039670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HOD88102.1"
                     /translation="MKKTYKFIAVLSFLFLFSFVASAQKVYSCEYKSDADVKVYVATY
                     KSDADLVVYKCNYQSDATDNKGLWYFCNYKSDAKKKIYFCEYKSDADLVIYFTEYKSD
                     AGWQNNSKKHLMY"
     gene            7905..10136
                     /locus_tag="PKN53_03775"
     CDS             7905..10136
                     /locus_tag="PKN53_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88103.1"
                     /translation="MALKVRKGYIIALIVIAIIFTGFYSLKFFFKKSLEEELNKVFNA
                     QVKIGKIDLKFFAGQIQIRDILIIGQGEFENDTLISCEKLRLDLEDLDKKTNSIVFGN
                     ILLDNLTIKNIISENENLCWDNILNKEIIEKDSLYKDDEAFQKMSVKFRQIVLTDGII
                     ENIDRKNNKEQKLSDIQFKLELNSDVEDLEANFNLECLYSTEMQNELKCRVNGDFVSF
                     KDDLSANAKICLNEFPLSCKFFMNTDSLANSKITINANADLSNIKSKTNNYKGEILLD
                     LEALACFENMSVSKMDYCLLADSLVIKNKNTLDSIFINFEYDVKLNNSESFANSSSLK
                     NLNVCSAKENLTGDFFMELSDTNYCLSSSLNGEFSYKNFAKILELENKNFDFNLEAKS
                     DLKYEYNLHKNSKQGDFLVSSNFTSPVFEMTNAEIEFKNNEFVLNSDFSSKYSKGDFV
                     INFGDFTNYIKQKPILFASECNFSKLIIPKFEIDEKKVSSKTIPSFSSENFKPYISDK
                     LKLSTTCKIDTLNIFEKDIFNIETNFIMDSEHFVFKSNKIILEEGYLQFNFEKLKKGE
                     DNLFITEFDLNNFALNYFLDEDTSISGWLTANIQNTIYLDKEKSAKSYGENKIGLKQF
                     QQQSDIFGEYGIADEKYLKISDFEAKFILKNDSLNLLPFRMKLNDLNADLVGDIDLKL
                     KKLNFNILLDIPEHYYTNLVKVALYAFSEKSEVKLPKKPKRIFRLLKIHGDFEKPKFV
                     IYE"
     gene            10165..10458
                     /locus_tag="PKN53_03780"
     CDS             10165..10458
                     /locus_tag="PKN53_03780"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOD88104.1"
                     /translation="MRRSETIRLGDLVRQAIKENNLQDGLDADRIKTIWAEETGEHIA
                     KLTKEIVFDKGKLYVSIKSSSIVRNEILLIRNELVKRINARLGRKFVKEIIVR"
     gene            10467..11501
                     /locus_tag="PKN53_03785"
     CDS             10467..11501
                     /locus_tag="PKN53_03785"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOD88105.1"
                     /translation="MIIEKFKNQIEILEDKISKSKKVKILPHYNPDGDAIGSALALFI
                     ILKKMGKDVEVISPSQFPGFLNFLPFANKINVIGKRREIDDNMFADVDLLIGVDFNSL
                     GRLDRLQLAFEKSSAYKIVIDHHPNPEQFSDLLISDTTRSSAAELIYDLIMATNLKNE
                     FDKDVATNLYVGIMTDTGSFSYNSSSPKTFEIVGKLLEAGVEKDKVFDEVYNSFSFDR
                     MRFKGHVMLNRMRIFPELKTGYIYITAEDRAEFNEKYGDTENFVNIPLSIEGIIFSAI
                     FIERENFVKISFRSKGSFDVNKFAAKYFNGGGHINASGGESYDSIHETVKNFVSILDN
                     YSNELKNYEF"
     gene            11491..12078
                     /locus_tag="PKN53_03790"
     CDS             11491..12078
                     /locus_tag="PKN53_03790"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOD88106.1"
                     /translation="MNFKITYLYFIAISILVIACSGGRKEQEKEKVVPYSEEKKKHRQ
                     ELLDWNKDITQVDYVVIEKFIERRKWDMQTSETGLFWQIYFKTEAEKAEPNMIAVLKY
                     TISLLDGSVVYTSDEFGLREIHLGHEVDEKGLSEGVLKMRKGEKARFIAPPYLAYGVP
                     GDGYRIPYYSSLVYDVELVDLYFPEDEVVLEDGWY"
     gene            12089..12946
                     /locus_tag="PKN53_03795"
     CDS             12089..12946
                     /locus_tag="PKN53_03795"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOD88107.1"
                     /translation="MKYLVFVFTILVLIISCGRNELKFQKHKSGLEYNIIEKINEDSL
                     LKVGDLVSLHLSYETEDGKEIFNSKNSERKYLRTIAKPAHIGGSFEDGMLMLGAGDSA
                     VFRVHAESFLRFSEMFANLPEGITYEDYLIVKVRVLEKITKEDYSKILAEKYHQSEEA
                     EMRFLQKYLDNFNITEEPEESGLIYVERVKGKGKQAVEGSFITVHYSLMLIDGDLVET
                     TLGREPFRFQLGNKNVIQGWEEGVAMMREGGQATLIIPSKLAYGKEGKGNILPYTTLI
                     FEVELLKVE"
     gene            12947..13951
                     /locus_tag="PKN53_03800"
     CDS             12947..13951
                     /locus_tag="PKN53_03800"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOD88108.1"
                     /translation="MQKIIIFIVLFLVLGFGFSCKNKKSENQEKTDLKKKPELKYNAD
                     SASESAAVLVFGNDGKVEYVEGGTDKAYQKTKSGLEYRIITKSKSKLRPKIGDVIFIN
                     MSYKTESDSILFKTSDIDKNFKMRLTTPSHEDGCIEEAFLLMYEGDSAAFRIDAYNFF
                     TYTQGKVNLPKNIKKGDKLIFNIKMLKIVSDAEYVMKNKDMYAYYINQEDSHIERYIL
                     NFEYPAVKTESGLRIMTIKQGSGKKVTKGQKVQIHYTAGFIDGGVFDSSLERNQAFEF
                     VVGENQVIEGLEEGVSKMNVGGHAILIIPFRLAYGEEKYGIIPPFSTLVFEIDLLDAK
                     "
     gene            13941..14534
                     /gene="nadD"
                     /locus_tag="PKN53_03805"
     CDS             13941..14534
                     /gene="nadD"
                     /locus_tag="PKN53_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOD88109.1"
                     /translation="MQNKIEILLFFGSFNPIHNGHLAIASYFTEFYGMREVWFVITPQ
                     NPAKQNENLLGNRERQHLVNLAIGDYPKFRVSDIEFNLPKPNYTINTLVYMEEKFPNK
                     KFSLLIGGDNLETFHKWKNYKLILENYKLYVYKRPGAIIPDLKSFGEKADIEIVDAPM
                     MEISSSFIRKAIKEYKDVRYFMPEKVYKYIKDMRYYL"
     gene            14534..15421
                     /locus_tag="PKN53_03810"
     CDS             14534..15421
                     /locus_tag="PKN53_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOD88110.1"
                     /translation="MKKADGQIDRVRAGYWAAGISVVVNILLFALKYWAGVKTGSIAI
                     IADAWHTLSDSISSIVVVFAIFLSSRKADKKHPFGHGRWEQIAALFVGFILGIIAWDF
                     LSSSVKLFSEHQAVHYGKIAIYATAISIVVKEGLAQYAFYIARKTKNLSIKADAWHHR
                     SDALSSVVVLAGIAVAKYFWWIDSVLGIIISLMLFYATFEIIKEAISKLLGEEASDDL
                     INKVKEIISKNYIHDVEAHHFHIHNYSTHQELTFHIKLNPDYNIAEAHKIASAIEKDI
                     LNEMNIHTTIHVEPMDKCE"
     gene            complement(15424..16410)
                     /locus_tag="PKN53_03815"
     CDS             complement(15424..16410)
                     /locus_tag="PKN53_03815"
                     /inference="COORDINATES: protein motif:HMM:NF016338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOD88111.1"
                     /translation="MLIYEKYISQIFDIKSEEEFANLCLQAFEYQYKNNEIYKRYCNL
                     LKIKTVNKINEIPFLPISFFKTNKILTKTKDFEVIFSSSSTTGSIPSLHYVSDIKVYE
                     KSFVKNFEYFYSSPSNYVILALLPSYLEREGSSLIYMAKDLINLSQKPESGFFLNQHE
                     ELHQIIQSLEKKSQKYILLGVSFAILDFAEKYKLNLNHGIIMETGGMKGRRKEITRNE
                     LHKILKTAFSCENIHSEYGMTELLSQAYSKKDGIFECPPQMKVLIRDPYDALSVKTIG
                     KGALNIIDLANINSCCFIETSDLGSVFSDGSFEISGRFDDSEIRGCNLMISE"
     gene            complement(16504..17304)
                     /locus_tag="PKN53_03820"
     CDS             complement(16504..17304)
                     /locus_tag="PKN53_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88112.1"
                     /translation="MKTKIIFFSAFCSLFCSSVLFANELVLSGIYQGKNLYFTNPSLG
                     ENEGYCAYEIIVNNTVYNSTLNSSTFGIYLDGIGLKFGQDFKIVIKHHEGCSPTLVNP
                     EVLQPISTFSIIESNLELNDNLVFKTKDEAGKLVFHVEEFRWGKWSEIKRIPGEGGPQ
                     ENEYSVKVYPFNGENKFRVYQIDDKYKKHYSEEMVFNVEKEPVKVLTKLHKVKKEIVF
                     SDVTRYAIIDDYGAELISGQGKNVDVAELKKGNYFLTFDNEYVVIKKK"
     gene            17601..19406
                     /locus_tag="PKN53_03825"
     CDS             17601..19406
                     /locus_tag="PKN53_03825"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOD88113.1"
                     /translation="MKKAVLSFIILLNLGLFAQEYSDYVLNYPNGNIKEKGVLHLGLK
                     EGTWEYYSPDGKLMAKVDYHKDMLHGENIIYYSDGKVKEEYNYWANERHGRFYEYYQN
                     GKPSLKGFYHSNLKDSVWQVYYENGNPQLEVLYRKGLRIKILNAYDLEGKQIVKDGNG
                     QMINYFNSLKIQSQGNLEDGVEQGLWTFYYENGVKSSEGNFEKGKRVGKWEEWFESGK
                     KKSELNYESGLNIYWFESGKKEMEGTLIDSKREKSWTYWYPSGQVRSINNYVNDLRDG
                     VQIKYYENGNKQSEINYKNGLKEGEAHFWFEDGKKDIDGNFKDDLQDGLWTYWRTDGS
                     KGNEGHFVRGKMEGRWNFWYANGRLWKEVDYAAGIKDGVWNIYFENGNKFREGNFEND
                     FENGLWKEWYDNGVLAMQGNYDLGIMVGNWKTWHENGSKKSEVEYTDGVLSGKNYFWY
                     PNGKKQVEENYKIVKEKSSGKFREEERITSVLHGSYKTFNIRGTEIISGVYYEGLRNG
                     KFVYNNDNGNTVKIEFYKMDKPDGKWQTFYDFGALESEVNYKNGVKEGKSTYYDQRGN
                     ITFQSMFRGGRQTDVIVDDNPNLPQNKGGQNQYRR"
     gene            19403..19981
                     /locus_tag="PKN53_03830"
     CDS             19403..19981
                     /locus_tag="PKN53_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88114.1"
                     /translation="MIRILLSGLLIFFVLNLSSQENKNMYSGGMLFFQPGYVSASNSY
                     QKISNFSTGIGGMLRFYFKKHLTLGIHGGSNKAIYKSKNSENSYISFAYGGPFVGFTA
                     QKNKLRFCSSLGIGMGKLRNLHIESQTETVLNESELFFYSGVSLNPLLSFDYNFTEKI
                     ALTTQLMTIITLYNKNDLYFCPTLQIGVLFNR"
     gene            19991..21529
                     /locus_tag="PKN53_03835"
     CDS             19991..21529
                     /locus_tag="PKN53_03835"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOD88115.1"
                     /translation="MNRDELFKKIIAHCKEYGFIFQSSELYDGLSAVYDYGQNGVELK
                     NNIKEYWWKAMTQLNEEVVGIDAAIFMHPTVWKASGHVDAFNDPLIDNKDSKKRYRAD
                     VLIEEHIEKINDKIKKEVEKAAKRFGDAFDEAQFVSTNPRVLGYAQKIQEIKSNFVSA
                     LENNDLEKLRQIIIDEGIVCPISGSRNWTEVRQFNLMFDTKLGSLSEGANTVYLRPET
                     AQGIFVNYLNVQKTGRMKIPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQYFVKPG
                     DELTYFEAWKEKRMKWHLAMGIALEKYRWHEHDKLAHYANAAFDIEFDFPFGFKELEG
                     IHSRTDFDLKQHEEFSGKKIRYFDNETDESYIPYVVETSIGLDRTFLSVLSAAYNEEQ
                     VGNETRVVLNLPPALAPVKVAVLPLVKKDGLPELARKIMNELKFDMNCEYEDKDSIGR
                     RYRRQDAIGTPFCITVDHQSLEDDTVTIRYRDSMEQERIPINKVASLVREKVDIKNLL
                     KQLV"
     gene            21558..22448
                     /locus_tag="PKN53_03840"
     CDS             21558..22448
                     /locus_tag="PKN53_03840"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOD88116.1"
                     /translation="MKNPKPIVLYVLLLISMALWGTSYVWGKMALEDYSAFTVIFFRL
                     LVSVIVLLPVLFLTKRFKLPNKKNLHLFIILAFFEPFLYFIGETNGLKYVDASMTSAI
                     IAIIPLFTPFVAYYFLKEKIRAVNILGILISILGIIFLVFDKHMNLQVPLKGLLLLSL
                     AVVAANGYSVIIKKMPLDHSVLNILFWQNLFGLIMFLPIVAIFDIQAIKATGFVYDSA
                     IAIVNLGIFASTIAFLFFIYALRFMPITKANVFSNTIPIFTLIIAWLVMDEPLSIRKI
                     IGVFIVIAGVVVSQLRYKSS"
     gene            complement(22503..23528)
                     /gene="dusB"
                     /locus_tag="PKN53_03845"
     CDS             complement(22503..23528)
                     /gene="dusB"
                     /locus_tag="PKN53_03845"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOD88117.1"
                     /translation="MLKIGELEFCDKPVFLAPMEDVTDPSFRYMCKKFGADLMFTEFI
                     SSDGLIRDAKKSVKKLDIFDYERPVGIQIYGHIPEAMQQASLMINENANPDLLDINYG
                     CPVKKIANRGAGSGMMRNLPLMKEITELVVKTSKFPVTAKTRLGWDSDSLNVEEVALL
                     LQDCGIQALTIHGRTRTQMYKGEADWSLIGKVKENQNIKIPIIGNGDITDAQNAYDKF
                     NKYGVDAIMIGRAAIGKPWIFAEVKHFLNTGKHLPSPGVIERVEYAKEHLQKSLEYKE
                     DKKGIYEMRRHFALYFKAIPDFKETRLKLLTAQTSEEILEILNFIQDKFGQYDSNFFE
                     NLNEHNK"
     gene            complement(23545..26508)
                     /gene="infB"
                     /locus_tag="PKN53_03850"
     CDS             complement(23545..26508)
                     /gene="infB"
                     /locus_tag="PKN53_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOD88118.1"
                     /translation="MTSDKKPVRLSTLARELNVGTSTIIEFLAKNGYTIEGGPNSKLP
                     FECVELLEEEFKESKTLKEKAIKNTEQARETKEMNLSSDKQAKISELAKEKELETVEK
                     ATIAEAEQVTTTESEEKSSTQTESSAEEKTTKPARAKHEVKPIENSETTANTTSPKVV
                     GTIDLDQINQKTRPKRKTKAEREEERKQKEEERKLKEEAAKKKKEAEAKKEKTKATEE
                     KKTPETKKQPETTETKKKVEKEKPAEKPRVFKALTVSEEAKQAEFIKTKIEKLSGPSV
                     VGKIDIDSFLTRQEESEKRRRQEKEERQKTREKEKEERKSKRKRIKKVKVNIDDDPKP
                     SEGRRRRPNKKNLKPAKPSIDQEDVQKQIKETLARLTTPKQKSRTSKYRREKRDLVSQ
                     KRQEEFEKQEAEKNILKVTEFVSVNEMSIMMDVPVNKLIATCMSLGLFVSINQRLDAE
                     TMALVADEYGFEVEFVSAEVLSEIEEEEDAEEDLLPRPPIVTVMGHVDHGKTKLLDRI
                     RNTNIIDGEAGGITQHIGAYSVKLPSGRHITFLDTPGHEAFTAMRARGAQITDVAIII
                     IAADDSVMPQTVEAINHASAAGVPMVFAINKIDKPTANPEKIKEALANMNYLVEDWGG
                     KYQSQEISAKAGININELLEKVILEAELLELKANPNKNAVGTVIEASLDKGRGYVTTL
                     LVQAGTLKVGDILLAGCQTGRVKAMYNERGNRVEEAGPSTPVLVLGLNGAPQAGDKFH
                     VMESDHEARNIANKRLQLQREQGLRTQKHITLDEIGRRLALGNFNEINLIIKGDVDGS
                     VEALSDSLIKLSTDSFAVNIIHKAVGQISESDVMLATASNAIIVGFQVRPSSHARKLA
                     EEEEIEIRLYSVIYDAIEEIKDAMEGMLSPTIKEKVTGSCEVLEVFKITKVGSVAGCM
                     VTDGKIHRKSKVRLIRDGIVIYTGELGSLKRFKDDAKEVTAGLECGLNIDKFNDIKVG
                     DIVEAFDEIEVKQTL"
     gene            complement(26535..27773)
                     /gene="nusA"
                     /locus_tag="PKN53_03855"
     CDS             complement(26535..27773)
                     /gene="nusA"
                     /locus_tag="PKN53_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOD88119.1"
                     /translation="MDLQNNLIENFAEFKELKNIDRPTMIGVLKDVFTSTLLKLFGEE
                     SKFDVIVNDANGDVQIWRTRTVVENVDFEDENTQIPLSEAQKIDEDYEAGEEFSDEIK
                     LQDFGRRAIMALRQNLKAKIAEREKNNIFLKYTDKINEIVVGEIYQIWKKEILILDDE
                     GIELILPKAEQIPNDRYRKGDTIRAVVISVSIEKGSPLITLSRTSPVFLERLFELEIP
                     EILDGLITIKKIVRIPGERAKVAVESYDERVDPVGACVGMKGSRIHGIVRELHNENID
                     IINYTTNTQLYIQRALSPAKISSIKIKPEENKVDVYLNPDQVSLAIGKGGLNIKLASQ
                     LLGMTIDVYREMDEDEDDVELDEFSDEIEQWIIDQFKAIGCDTAKSVLNLSVEDLVKR
                     TDLEEETVIEVLNILKSEFE"
     gene            complement(27796..28254)
                     /gene="rimP"
                     /locus_tag="PKN53_03860"
     CDS             complement(27796..28254)
                     /gene="rimP"
                     /locus_tag="PKN53_03860"
                     /inference="COORDINATES: protein motif:HMM:NF002531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HOD88120.1"
                     /translation="MIDKKEIENLCKSALTETQFLVEIKISNNNDIFVTIDDFNGLNI
                     EECRRISRFIEENLDRDKEDFSIEVSSPGLSNPFKVPEQYQKSLNKEVEVILKTGEKI
                     TGTLTTIRDDEIIISYSYFAKMANKKQQISEISKIKFKDIKSTKSVISFK"
     gene            28447..29334
                     /gene="lgt"
                     /locus_tag="PKN53_03865"
     CDS             28447..29334
                     /gene="lgt"
                     /locus_tag="PKN53_03865"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOD88121.1"
                     /translation="MDFLFITWNVDPVIFYLGNRGIRWYGLLLAIGFLVGYLLLSRQM
                     KKEGVQQLVVDKLAIYVIVGVVLGLRLGHCLFYNPGYYLSNPIEILYVWEGGLASHGG
                     AAGILLAVWLFSRKFKINYLSLLDRVVLTVPFAGGMVRLGNLMNSEIIGVQTSMPWGF
                     KFLRNTEDLIPAIKASGGKCGFEDLACLAEFWTPRHPTQLYEAIFYFIMFVAFYFIYQ
                     KYIQKWKPGVFLGWFITVLFVFRYFIEFTKVDPVEFDGWTDAIRMGQLLSIPFVLLGL
                     FLMGRGYGWFKRKAKVKKE"
     gene            29346..>29767
                     /locus_tag="PKN53_03870"
     CDS             29346..>29767
                     /locus_tag="PKN53_03870"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOD88122.1"
                     /translation="MRDFEFYNPVKIIFGKNKIANIAEELKPYNKIMFTYGKGSIKRN
                     GIYERVVSALEGKDFIEFGGIEPNPSYETLMKAVKLGKENGVDFLLAVGGGSVIDGTK
                     FIAAAIKYSGDDPWDMIAKGEKVLDAVPLGSVLTIPATG"
BASE COUNT         9757 a         3977 c         4848 g        11185 t
ORIGIN      
        1 aagaattaca atcaaaagta ttgataaaaa cgcactaata ggaaattttt tcataacttt
       61 aattttacag gttattattt gcttttttaa atcttaatat tcatacaatc taaaatttta
      121 acaaatatat gtaaaatttt tgtaaaacaa tttgattaat agcaactata tttttatgag
      181 taacaatttt gtttaaaata tttaagttgc ctcattgaac ttctgatgca atatctgctt
      241 cttcccaatt atctatgttt gacaaaagtt cttttgaatt aatttgcaaa tttctagctt
      301 ttagaattgc ttcagattca ttttctgcaa gtatttcgta agtacaaaaa cctgaatagt
      361 gaattttgac ttcgtatttt ttagtttttt tgttcattat gttaatgttt taattgtatt
      421 aagggttgaa aataaatggt atgtgtattt tattcttcgt caaataattc tgctggattg
      481 attttgaatt gcaacatgtt tgctgtattt ctgcctccat cgccaccttt tctttgcata
      541 gtgattttgc ctattctaaa acttcctcta ttagtaatta taatttctcc gtttccgaaa
      601 taattcatgc aaaaattcat tggtttaaga acccaccttg catttttatt gatcttttga
      661 gcaactaaca tccactctgc ggcaaatttt cctcgtcctt ataatatatc tgaaacgatt
      721 aaagattggt ttttcttcaa ccattttaaa acaacttttt gttcatcttc agaaaattca
      781 tttgcaaaca ttcttctttt gtcttttgtc tttttaattg taggtttttc ttcacctgta
      841 aatcttttta gaattgaaac aacttgcaaa gggatattcc acatttcagt atatttatca
      901 acccatcgtt tatcaatttg gttaaatcct ttggggttac ttactaattt tacttgtaag
      961 ttttccacgt ctattgcttt tttgagtttt attgttactt gtacttgtac atcagttttt
     1021 ccgcctagat ttgcagataa tttaacagct tcaacaaatt cgatttcaga cagtttgtat
     1081 ttcattaaaa taagccaaga ttgagctatt ttatcttttt tccaattgtt aaatttttta
     1141 caatgtcatc ttcattttta aagccttctt ttgctgtttg agaacctttt tctactaaat
     1201 tcatattata ttgaagtaaa gagattattt atagtgtatt cttttaatct ttcttctgaa
     1261 attttaacgt attcttctga aatttcagag cctatccatc ttcgctttaa tagatgagca
     1321 attttagcag tagtaccact tcccataaaa gggtcataaa ctaaatcgtt ttcgttagtc
     1381 catgttttaa tttggtcggc aacaagtttt tcaggaaaaa ttgcaggatg gttatatgct
     1441 attttgtctt ttgttgaaat tcctccgccg acagaataaa aaaacacatt accaactttc
     1501 ttttcttttg ttctttctac tttttcatag tcaagactgc cattttgtcc tctgtttttc
     1561 atgttggcaa gtccttgata tttagtgggt tctgttattg ggttaaatgt tttaggacta
     1621 ccttttgaaa aagcaaacat atattcaaaa tgttgatgat atctatttcc tgtttgtggc
     1681 ataggattat ttttataata tatcattgta tcatgtaatc tgaaaccaat ttctataaaa
     1741 aataatgctt gtttaaacga tgtccctgtt tcacttccgt taattgttgc atctgctaca
     1801 acccaaataa ttattccacc ttgttttgta atgcgaaaca attcttttgc tattttttca
     1861 aattcaaatt ggtaaccatt ataatttcta atatcatcat aaggtggtga tgtgataaca
     1921 aaatcaacat aattgtcggg catgttctgc attgtaatta aacaattttc attatatgtt
     1981 ttttcaagtt ccatgctgca aatttagtta atttctgtca acatttctaa taatgtttct
     2041 ttgatttatt caaaacccta aatatttaaa attatctttt atttcgcaaa ccctacagtt
     2101 tgtgctaata gaatttcttg attttcaggt aattgcaata ttttgtgaag ttcttctttg
     2161 tcgaaagctc ctcttgcaac agttcctaag ccttctgaag cacagtacag ataaatattt
     2221 tgagaaatat atccacagtt tgtgtaagag taatgtgaac tagctgcctt ttcagtgtcg
     2281 gcaacaatga gaatgttaac tgctgcatcg gctacaaact cttgcaaccc tgcgtttttt
     2341 ctataatcgc cttttattgt caatgtaagt ttgtgctcct tggcattgta aaaataaata
     2401 ccatctttta aaattacata aagttctaat tcttgacaat ttctagctgt aggtactgtt
     2461 cgtttatcat ctctgttaaa accattagct gcccatagca aatttgacaa ctgctgattt
     2521 gataattctt tatcagtaaa cgaacgagta gattttcttt catttaaagt ttccatcaaa
     2581 gtttttccgc cctttttgtc tggtgctggt aatttaattt cttgtgcatt catattcata
     2641 ctaaatccta aatagattaa aaaaataact aaaacttttg ttctcataaa tttatgattt
     2701 tttaaaattc ttgctcaaag ataataaaat attttgacat ttggctattg taacattgct
     2761 ttttttaaaa taacgataga attgatttaa ttgagtaaaa acttgtgtta ttaataattg
     2821 tttttaaaat cattaattat ctgatttgta taattttcca tatcattaaa attatctgtc
     2881 ttaagtgtta cgtcaataaa ttcaggaatg gaatatggtt ttatattgat actttgctct
     2941 ttgctcttgt gtttaactat tgaaaaatcg taaaattctt ttgtgtcttg gtgaatgatt
     3001 ggagctacaa aagaaaggta ttttgacaaa taattattaa ttttgttatc ttctaaaaaa
     3061 tggcgtatgg cagtagtagt ttcactattt agttgttgag ttttattccg aattagagtt
     3121 acctcaatta gccaatatat attctttgaa taaacttcaa tatccccctt attcccttgt
     3181 gcatgcgaaa ttggtaagcc aatataatca gctttatagt taggatttat ggcaaacttt
     3241 tttccatatt ttaatgttaa aatcaaactt aaataaaact ctagttttaa aggatctggg
     3301 atatacttaa atgctggaat tatgttcttt gtagtcccga tggcattaat gctttcaaca
     3361 attatctttt cattaagatt gtacatttta ataattttcg atactttttg aacgtattca
     3421 atatctttct ttttagcaat ctcttctctg tgtttgttaa ttattttaag taattgttta
     3481 ttgtatgttt caagtttcgt aaaatgtaaa tttggatttt ctttttcagt ttcagttaac
     3541 tctatattta ttgctaataa atcatttatg taattgtcgt ttgcaatatt tctgtaaaca
     3601 aaaacttttc cactaaactg aatggaaaca aaacctgtaa taattaaaag gcgaagtatt
     3661 acatcggggt aatcacgtaa aatagtttgt gtttttagat ttgtttcata ttttgatctt
     3721 aaaaatgttt caacttctga caaatcagca aaagagtttt tatttataag ttgtaaaaat
     3781 tcaccaacat ctccattggt actgaaagtt gaaataataa attgttcgta agaaattcgc
     3841 tcacgttgtt ttaaaatttc taaaataaac ttaaaataat tgaagtcgtt taaaacattg
     3901 cgatatggag aacgcctgtt gtatttcatg gcttgagttg aaaatgcttc ttgttcgtca
     3961 atttcattgt ttatgagctt tatggcaata ttagaaaatt ttaatggttg ttgatattgg
     4021 gcaaaaacaa atcccatttc agacaaagtt ctgacataag tccaaaaacg cgactgatag
     4081 ccttcgggga aaccgccgtc agctctacgt gtactgttta cactaattac tgcgtcagca
     4141 atattctcaa tagttgaatt ttcatcaatt attacatttt tactagaaac aacacctttt
     4201 aaatataatg aagaaacaac ttgcaaaagc gtttcatcat tgagtacttt gtttatatat
     4261 ggtaatatcg cagttaagat ttctttatag cgttctgggt ttcgaacagc tgtttcaaaa
     4321 gccatttgtt tgtaagtcgc ttgtctttta ttttttttgt ttgtcattat tatggttttt
     4381 tatagtgtca tgttagattt gtaatttgta actaaaactt cattaatttt tttgatcgta
     4441 ttatcatgat agctaatata gttgcttttt atttgataag tattgtactt gcttgaccaa
     4501 tcaataaata aactattttc tttttctttg tagtgagtaa tgtttgaaat agcaaattta
     4561 atattttctt tatctaaaat atccagtgtc tgcaatagtt cgttttctgt ttcttcgttc
     4621 caaagtttat tatattcact taatgtgatt aaataaggtg gatctaaata gataaaatca
     4681 tttttttgaa aatttattga ttttaggaaa gttttaaaat ctaaattata ccaataagct
     4741 tgtttagttt tgtttagttt aaagtaatcg gatagcgcat taaaaacatt tctgttaaaa
     4801 tctatgtttc ctacaggtaa attatagtta ccttgattgt taaaacgtaa cattctatta
     4861 aaaccataaa ttaaaagtac atataaaaca gcaatatctt tttcttttga ttgattataa
     4921 tcttttttta gacgattaaa attttcttta ttaaaatgtg catagtatgt ttttttaaaa
     4981 cgttttttta attcaaaagg gacaatgtct ttttcataag ataaagacaa attgtattta
     5041 tgaatgatag aaaaaattaa atcataaaac tcgttttcct tttcaacaaa tgagcataaa
     5101 aacttatgaa tatcaataac atttttgtca atatcgttaa gtaaatattt ttttgcttta
     5161 acgttaagat atacagagcc accacccaca aatggctcaa taaattgatt aattgagggg
     5221 gggaaatgtg tttttatctc ctttataagt ttgtatttat cacctacata gaagagaggt
     5281 gagcgattta ttttttcttt tttcatctac ttcggtaatg aataatagtt ctttatggtt
     5341 atcaaactct gttttccctg tattgaaaaa acgatgtgag cattcgaaaa tttttgtttt
     5401 tccgcattta tttaaaatat attctatttc ctctaatttt attttgtttt cagaagaatg
     5461 acttttagaa ttgtatgtat tgttgtaaga tactactaaa aaattggtgt ttaaatgtaa
     5521 tactaaatgt ttaaatgcgt tttttgcttt tgttgtacaa taaaggctgt tgttttcggg
     5581 agctggcttt agggcaacac cataaagttt gggtttatcc cattttacaa gcgtttcata
     5641 taaatgataa aatctattgt actgccttga attatatgga ggatcaatgt aaactaaatc
     5701 agaatttatt tgttgggcaa gtttatttgc atcttcacga tagattttta cattatcaaa
     5761 atcttgagca tctattaatc gtagtcgcaa tggtcgaggt tttatgggtt ttttaatata
     5821 agcatcaaag tgcccaacgg tattggctaa tttgtcaata ttgtaaataa gtgtggttaa
     5881 aagtatattg tactctttat cagtaagttc tggttttaaa ttttctatgt cttgtctaat
     5941 ataaccgatt aatttagctg tgttatgttc gtaaaatttt ccgccaaaat tttctgaaaa
     6001 ataattttca tcaattaaat cagcatctaa tctattgtag tttgtaatta tatcgtttag
     6061 tttagcttta tcccatttgc ctttttttaa aaaaccttta taaatcacat tgtttgcata
     6121 aagtatatca ttgataatta cttgattata tttttgtatt gcttgattag atacagatgc
     6181 agtacctgca aaaatatcta caaaagtgtt gatgttttca gtttctttat ctatcaaatt
     6241 cataatccac tctgtgagtt ttgctttact gccaatatat cggcgacttt ctattggtat
     6301 tggtttttga taaatagtat cttgaaaaag ctgaatgtat tgattcccgt attttatttt
     6361 atcttcggca acttttaatg tttctgtagc aagttctgga tattctaaag aattagcaat
     6421 tttatcgctt tctctttgaa taattaaaag tttgtcatcg taattataaa atttagacaa
     6481 tttttgtaac tgttcaattg tgggcagacg tttaccattt tcaattttac ttagtagtgt
     6541 taaatctatg cctaattctt gttgcacttc ttgcaaaggg agtcctattt cttctcgtat
     6601 ttcttttaat aaatgtccta ttgtgtacat gttttatttt ttgacatcaa aaggcaaaaa
     6661 tacaaaaatt tttattttat aacaaaacac aaaatcattt ttctactatg tcaataaaaa
     6721 atctaaaccc aacaaaaggc aaacttgctt ctttatattt ataatcaaaa tcagaaggat
     6781 taattccatt ttcaaaatca ccacctttag catgaccttt agtgttagtc atttctttaa
     6841 cattttctaa aacattgaaa aacttaccag tatttttgta tttatcaact gggcgaacat
     6901 tattgtctgt ttgttttttg ctagctttag actcgatttt tgaaagaagc atattccatt
     6961 cttcacttga tggtaagcgt atttcaactt ttttatattt tctatcagaa taattgttgt
     7021 aacattcggt aagccatttg caaaattcaa cagcagcttc gtaacttata ttaacaactg
     7081 ggtaatttgc aagagcagga tgcaaatgat aatattgagc ataaggctca ttgctatatt
     7141 ttggtaatcg ccaatttgca gagtcaactt tcaataaatt aatatcgcta cctgtttgct
     7201 ttgataaaaa taaattgtaa agctcattgc ttgtttcata tttataggca taaagattgt
     7261 catttacttt tataaaattt ttttctattt cttttaagga taatatagaa ggctggcaaa
     7321 aactataaca agtaaggaac acagtaattg tcaataaggc aagttgtttt gttttcataa
     7381 taagcagttt taaaattata caaatatatg gttattctac aaactttaaa aataaatatt
     7441 tattgtatca agttttcaat ttatttctta tcttagcagt tttatatggg caaagttttt
     7501 gccgaattat taataattaa aaaaaatcgt tatgaaaaag acttacaagt ttattgcagt
     7561 tttgtcgttt ttgtttttat ttagttttgt agcaagtgct caaaaagttt attcttgtga
     7621 atataaaagc gatgcagatg tgaaagttta tgtggcaact tataaaagcg atgctgattt
     7681 agttgtgtat aaatgtaatt accaaagtga tgctaccgac aataaaggtt tgtggtattt
     7741 ttgtaattat aaatctgatg caaaaaagaa aatttatttt tgcgaatata aatccgatgc
     7801 tgatttggta atttatttta ctgaatataa atctgatgca ggttggcaaa ataatagcaa
     7861 aaaacatttg atgtattaat ttgacttaaa aaataaaatt acaagtggct cttaaagtaa
     7921 gaaaaggata tatcatagct ttaatagtta ttgcaattat ttttactggt ttttattctt
     7981 tgaaattctt ttttaagaag agccttgaag aagaactgaa taaggttttt aatgcacagg
     8041 tgaaaattgg aaaaattgat ttaaagtttt ttgcaggaca aatacaaatt cgtgatattc
     8101 taataatagg gcaaggcgaa tttgaaaacg acactttaat aagttgtgaa aaattaaggc
     8161 ttgatttaga ggatttagat aaaaaaacaa attctattgt atttggcaat attttgttgg
     8221 ataacttaac tatcaaaaat ataatttcag aaaatgaaaa tttgtgttgg gataatattt
     8281 taaataaaga aattattgaa aaagatagtc tttataaaga tgatgaggct tttcaaaaaa
     8341 tgtcggttaa gtttaggcaa attgttttaa cagatggaat aatagaaaat attgacagaa
     8401 aaaataataa agagcaaaaa ttgtcagata tacaatttaa actagaactt aattctgatg
     8461 tggaagattt agaagcaaat tttaatcttg aatgtcttta ttctacagaa atgcaaaatg
     8521 agctaaaatg tagagtcaat ggcgattttg taagttttaa agatgatttg tcggcaaatg
     8581 ctaaaatctg cttgaatgaa tttcctctta gttgcaaatt ctttatgaat acagatagcc
     8641 ttgcaaattc taaaatcaca attaatgcaa atgctgattt gtcaaatata aaatcaaaaa
     8701 caaataatta taaaggcgaa attttgttag atcttgaggc tttagcgtgt tttgaaaata
     8761 tgagcgtttc aaaaatggat tattgcttac tggctgatag cttggtaatt aagaataaaa
     8821 acacattaga ttctattttt ataaattttg aatatgatgt taaactaaat aattccgaga
     8881 gttttgcaaa ttcaagcagt cttaaaaatc taaatgtttg ttcagctaag gaaaatttaa
     8941 ctggtgattt ttttatggaa ttatctgata caaattattg tttaagttca agtttaaacg
     9001 gagagtttag ttataaaaac tttgctaaaa ttttagaatt agaaaacaaa aattttgatt
     9061 ttaatcttga agccaaatcc gatttaaaat atgaatataa tttgcataaa aattctaagc
     9121 aaggcgattt tttagttagt tcaaatttta caagtcctgt ttttgaaatg acaaatgcag
     9181 aaattgaatt taaaaataat gaatttgttc ttaattctga ttttagttca aaatactcaa
     9241 agggagattt tgtcataaat ttcggtgatt ttacaaacta tataaaacaa aaaccaattt
     9301 tatttgctag tgagtgtaac ttttcaaaac taattattcc aaagtttgaa atagatgaga
     9361 aaaaagtatc aagcaaaact attccttctt tttcttcaga gaattttaaa ccatatatta
     9421 gcgataaatt aaaactaagt acaacttgta aaatcgatac tttaaatatt ttcgaaaaag
     9481 acatttttaa tattgaaacc aattttataa tggattccga gcattttgtt tttaaatcaa
     9541 ataaaattat tcttgaagaa ggatatttgc aatttaattt tgaaaagctg aaaaaaggag
     9601 aagacaattt gtttataacg gaatttgatt tgaataattt tgctttaaac tattttcttg
     9661 atgaagacac aagtatttcg ggttggttaa ctgctaatat tcaaaatact atttatttag
     9721 acaaagaaaa atcggcaaaa tcttatggtg aaaataaaat tggtttaaaa caatttcaac
     9781 agcagtcaga tatttttgga gaatacggta ttgctgatga aaagtattta aaaatatctg
     9841 attttgaagc aaagtttatt ttgaaaaacg acagtttaaa tcttttgcca tttagaatga
     9901 aacttaatga tttaaacgca gatttagtcg gagatataga tttgaaattg aaaaaattaa
     9961 attttaatat tttattagac attccagaac attattatac taatttggtt aaagttgctt
    10021 tatatgcttt ttctgagaaa agtgaagtga aattaccgaa aaagccaaaa agaattttta
    10081 gattattaaa aatacacggc gattttgaaa aaccaaaatt tgtgatttat gaataaaaat
    10141 agtttttttg tttaaaatat aattatgaga agatctgaaa caatacgact tggcgacctt
    10201 gtgagacaag ctataaagga aaataacttg caagacggct tagatgcaga cagaataaaa
    10261 actatttggg cagaagaaac aggcgaacat attgcaaaat tgacaaaaga aatagttttt
    10321 gataaaggaa aactttatgt tagcataaaa tcatcatcta ttgttagaaa tgaaatttta
    10381 cttatacgta acgaattagt gaaaagaatt aacgctaggt tgggacgtaa gtttgttaaa
    10441 gaaattattg tgagataaat tattttatga ttattgaaaa atttaaaaat caaatagaaa
    10501 ttcttgaaga caagattagt aagagtaaga aagtaaagat tttaccacat tataatcctg
    10561 atggcgatgc aataggttca gcacttgcat tatttattat tcttaaaaaa atgggtaaag
    10621 atgttgaagt aattagtcct tcgcaatttc ctggtttttt aaattttttg ccctttgcaa
    10681 ataaaataaa tgtaattggc aaaagaagag aaattgacga taatatgttt gctgatgtcg
    10741 atttgcttat aggtgttgat tttaattctt tgggaagatt ggatagattg caattagctt
    10801 ttgaaaaatc atcagcttat aaaattgtta tcgaccatca tcctaatcct gagcaattta
    10861 gcgatttgct tatttccgat actactcgca gttctgcagc agaattgatt tatgatttga
    10921 ttatggcaac aaaccttaaa aatgagtttg ataaagatgt ggcaacaaat ttatatgttg
    10981 gaattatgac tgatacaggc tcgttttcat ataattcgtc ttcgccaaaa acatttgaaa
    11041 tagttggcaa actgctagaa gctggtgtgg aaaaagacaa agtttttgat gaggtttaca
    11101 atagcttttc ttttgaccgc atgagattta aagggcatgt tatgcttaac agaatgcgaa
    11161 tttttccaga attaaaaaca ggatatattt atataacagc tgaagataga gctgaattta
    11221 atgaaaaata tggcgacacc gaaaactttg taaatattcc attaagtatt gaaggaataa
    11281 ttttttcagc aatttttatc gaaagagaaa attttgtaaa aattagtttt cgctctaaag
    11341 gaagtttcga tgttaataaa tttgctgcaa aatattttaa tggaggtgga catataaatg
    11401 cttcgggagg agaatcttac gattcaatac acgaaactgt gaaaaatttc gtttctatct
    11461 tagataatta ttcaaatgag ttgaaaaatt atgaatttta aaattactta tttatatttt
    11521 atagccatat ctattctcgt aattgcttgt tctggtggta gaaaagaaca agaaaaggag
    11581 aaagttgttc cttattctga agaaaagaaa aaacacaggc aagaattatt ggattggaat
    11641 aaagatatta cacaagtaga ttatgtagtt atagaaaaat ttattgaaag acgtaaatgg
    11701 gatatgcaaa catctgaaac tggacttttt tggcaaattt attttaaaac agaagccgaa
    11761 aaagccgagc caaatatgat tgcagtttta aaatatacaa tttctttgct agatggaagt
    11821 gttgtttata cttcagacga gtttggattg agagaaattc accttggaca tgaggttgat
    11881 gagaaaggac ttagcgaagg ggttttaaaa atgagaaaag gcgaaaaagc tagatttata
    11941 gcaccaccat atttggctta tggcgttcca ggagatggat atagaattcc ttattactct
    12001 agtttagttt atgatgtgga attagtagat ttgtattttc ctgaagatga ggttgtttta
    12061 gaagatggtt ggtattaaaa tgaagaatat gaaatattta gtttttgttt ttacaatttt
    12121 ggttttaata atttcttgtg gacgaaatga attgaagttt caaaagcaca agtcgggatt
    12181 ggaatataat ataattgaga aaattaatga agattcttta cttaaagttg gagatttggt
    12241 gtctttacat ttaagctatg aaaccgaaga tggtaaagaa atatttaatt ctaaaaattc
    12301 tgaaagaaaa tatcttagaa ctattgcaaa acctgctcat atcggtggta gcttcgaaga
    12361 cggaatgttg atgttgggag ctggagattc tgccgtgttt agagttcatg ccgaaagttt
    12421 tttaagattt tcagaaatgt ttgctaattt gcccgaaggt ataacttatg aagattattt
    12481 aattgttaaa gttagagttt tagaaaaaat tactaaagaa gattattcta aaattttagc
    12541 agaaaaatat catcaaagcg aagaagctga aatgagattt ttacaaaaat atttggataa
    12601 ttttaatatt accgaagaac cagaagaatc aggtttgata tatgttgaaa gggtaaaagg
    12661 aaaaggcaaa caagccgtag aaggttcttt tattactgtt cattattctc taatgcttat
    12721 tgacggagat ttggttgaaa ctactttagg aagagagcct tttagatttc aattaggtaa
    12781 taaaaatgtt atacaaggct gggaagaagg ggttgctatg atgcgtgaag gaggacaagc
    12841 gacattgata attccatcta aacttgctta tggaaaagaa ggtaaaggga atattttacc
    12901 atatacaact ttaatttttg aagttgaact tttaaaagtt gaataaatgc aaaagataat
    12961 aatcttcata gttttatttc ttgtattagg ttttggcttt tcgtgtaaaa acaaaaagtc
    13021 tgaaaatcaa gaaaaaactg acttgaaaaa aaagccagaa ttaaaatata atgctgattc
    13081 ggcatcggaa agtgcagcag ttttggtttt tggtaacgat ggtaaagttg aatatgtaga
    13141 aggaggaaca gataaagctt atcaaaagac aaaatctggt ttggagtata gaataattac
    13201 aaaatcaaaa tctaagttaa gaccaaaaat aggagatgtt atttttataa atatgagcta
    13261 taaaacagaa tcagattcta ttttatttaa aacttcagat attgataaaa actttaagat
    13321 gaggctaaca acaccatctc atgaagacgg ttgtattgaa gaagcttttt tgcttatgta
    13381 tgaaggcgat tcagctgcat ttagaataga tgcgtacaat ttttttactt atactcaagg
    13441 caaagtaaat cttccaaaaa atattaaaaa aggcgataag ttaattttta atattaaaat
    13501 gttgaagatt gtatctgatg ctgagtatgt tatgaaaaat aaagatatgt atgcttacta
    13561 cataaatcag gaagatagcc atattgaaag atatatttta aattttgaat atcctgcagt
    13621 taaaacagaa tcgggattga gaattatgac aattaagcag ggttctggca aaaaagttac
    13681 gaaaggacaa aaggtgcaaa ttcattacac agcaggtttt attgatggag gtgtttttga
    13741 ttctagcttg gaaagaaatc aagcttttga atttgttgtg ggcgaaaatc aagtgataga
    13801 aggcttagaa gaaggagtgt cgaaaatgaa tgtaggtggt catgcgattt tgattattcc
    13861 tttccgcttg gcttatggcg aggaaaaata tggaattatt ccaccatttt cgactttagt
    13921 ttttgaaatt gatttattag atgcaaaata aaatagaaat attgctgttt tttggttcgt
    13981 tcaatcctat tcacaacgga catttggcta ttgcttcgta ttttacagaa ttttatggaa
    14041 tgcgtgaggt ttggtttgta ataacaccgc aaaatccagc taaacaaaat gaaaatcttt
    14101 taggtaatag agaaagacaa catcttgtaa atcttgctat tggcgattat ccaaagttta
    14161 gagtgtcgga tatagaattt aatcttccaa aacctaatta tacaattaat actttggtat
    14221 atatggaaga aaaatttcct aacaaaaaat ttagtttgct cattggtgga gataatttgg
    14281 aaacttttca taaatggaaa aattacaaac ttattttgga aaattataaa ttatatgttt
    14341 ataaaagacc gggggcaata attccagacc ttaaaagttt tggcgaaaaa gctgatatag
    14401 aaattgtaga tgcaccaatg atggaaattt cttctagctt tatccgaaaa gcaataaaag
    14461 aatataaaga tgtaagatat tttatgccag aaaaggtgta taaatatata aaagacatga
    14521 gatattattt gtgatgaaaa aggctgatgg tcaaatagat agggtgcgcg caggctattg
    14581 ggcagctgga atttctgttg ttgtaaatat tttattattt gctttaaaat attgggctgg
    14641 agtaaaaact ggctcaattg caattattgc tgatgcttgg cacactttgt cggactctat
    14701 tagttctata gttgttgttt ttgcaatatt tttgtcttcg cgtaaagcag ataaaaaaca
    14761 tccgtttgga catggacgct gggagcaaat agcagctttg tttgtaggtt ttattttagg
    14821 aattattgct tgggattttt taagttcgtc tgtaaaacta tttagcgaac atcaagctgt
    14881 gcattatggc aaaattgcta tttatgctac tgcaatttct attgtagtaa aagaaggcct
    14941 tgctcaatat gctttttata ttgcacgtaa aaccaaaaat ttatcaataa aagccgatgc
    15001 ttggcatcat aggtcagatg cgctttcgtc tgtagttgtt ttggcaggaa tagctgtggc
    15061 gaaatatttt tggtggatag attctgtgtt aggaataata atttctttaa tgttatttta
    15121 tgcaactttt gaaattatta aagaagcgat aagcaaatta cttggcgaag aagctagcga
    15181 cgatttaata aataaagtaa aggaaataat ttctaaaaat tacattcacg atgttgaagc
    15241 tcatcacttt catattcata attactcaac acaccaagag cttacttttc atataaaatt
    15301 aaatccagac tacaatattg cagaagcaca taaaatagca agtgcaatag aaaaagatat
    15361 tttaaacgag atgaatattc atacaacaat tcatgtagaa cctatggata agtgtgaata
    15421 gttttattcg cttatcatta aattgcagcc tcttatttcg ctgtcatcaa atcttccaga
    15481 aatttcaaaa cttccgtctg aaaaaacaga gcctaagtcg ctagtttcga taaaacagca
    15541 tgaattaata ttggcaaggt caattatgtt taaggctcct ttgccaatag tttttacaga
    15601 caaagcatcg taagggtcgc gaattaaaac tttcatttgt ggaggacatt caaaaattcc
    15661 atcttttttt gaatatgctt gcgataaaag ctctgtcata ccatattcag aatgaatgtt
    15721 ttcgcaagaa aaagcggttt ttaagatttt gtgaagttcg tttcgagtaa tttcttttct
    15781 tcttcctttc attccgccag tttccataat aattccatgg tttaggttta atttatattt
    15841 ttcagcaaaa tctaaaatag cgaaacttac acctaataat atatacttct gagatttttt
    15901 ttctaaactt tgaataattt gatgtaattc ttcgtgctga tttaagaaaa aaccactttc
    15961 aggcttttga gataagttta ttaaatcttt tgccatataa atcaaactag aaccttctct
    16021 ttccaaatat gaaggcaaca aagctaaaat aacatagttt gagggtgaag aataaaaata
    16081 ttcaaagttt tttacaaaag atttttcgta aacttttatg tccgaaacat aatgaagaga
    16141 aggaatagag cctgttgtgc tactcgatga aaagatgact tcaaaatctt ttgttttagt
    16201 taaaattttg ttggttttaa aaaatgaaat tggtaaaaat ggtatttcgt ttattttatt
    16261 tactgttttt attttaagta aattgcaata tcgcttatag atttcattgt ttttgtattg
    16321 atattcaaag gcttgtaggc aaagatttgc aaattcctct tcgcttttaa tgtcgaaaat
    16381 ttgactaata tatttttcgt agataagcat tttgattttg caaaaatagg aaaaataagt
    16441 agtttaaaag ctaagtatta agtaaaataa ttgagcagtt tttagaaaag tatttttgta
    16501 aaattatttt ttctttataa ccacatattc gttgtcgaag gttaaaaaat aatttccttt
    16561 tttcaattca gcgacatcta catttttacc ttgtccagaa attagttctg caccatagtc
    16621 atcaattatc gcatagcgag taacatcaga aaaaactatt tcttttttta ctttatgtag
    16681 cttggttagt acttttactg gttctttttc gacattaaac accatttctt cagaataatg
    16741 ttttttgtat ttatcatcaa tttgataaac tctaaatttg ttttcgccat taaaaggata
    16801 aacttttaca gaatattcat tttcttgagg accaccttcg ccgggtattc tctttatttc
    16861 gctccatttt ccccacctaa actcttcaac gtgaaaaaca agtttaccag cttcgtcttt
    16921 ggttttaaaa actaaattat cgtttagttc taaattgctt tcaataattg agaaagtact
    16981 aattggttgt aatacttctg gatttactaa tgttggggaa cagccttcgt gatgtttgat
    17041 aacaatttta aaatcttgac caaactttag accaatacca tctaaataaa ttccaaaagt
    17101 acttgagtta agggtgctgt tataaactgt gttattaaca ataatttcgt aggcacaata
    17161 tccttcgttt tcgcctaagc tcggattggt aaaatataaa tttttacctt gataaatacc
    17221 acttaaaact agttcattag cgaataacac gcttgagcaa aataatgaac aaaaagctga
    17281 aaaaaatata attttagttt tcataataaa aagttatcaa caattttatt aacaaatata
    17341 tataaaaaaa tttacttcca aatatttttt taaatatttt tcactttttt tatgattttt
    17401 tcgtgttttt cccaattttt tcgtgtttta agtatcttaa aatttttttt ctattgtttg
    17461 ccattaatat ttagttttgg aattttattt acatttaaca ttaattgttg aaatttttgt
    17521 ttacaagatt ttttattaaa gtggaaaatc gatttacaaa tgttatattt gtagattaag
    17581 aaatttataa attttacttt atgaaaaaag cagttttatc ttttataatt ttattaaatc
    17641 ttggtctttt tgcacaagag tatagcgatt atgttttgaa ttatccaaat ggcaatataa
    17701 aggaaaaagg tgttttacat ttaggattaa aggaaggaac ctgggaatat tattctcctg
    17761 acggtaaatt gatggcaaaa gttgattatc ataaagatat gttgcatggc gaaaatatta
    17821 tttattattc tgatggcaag gtaaaagagg aatataatta ttgggcaaat gagcgtcatg
    17881 gtaggtttta tgaatattat caaaatggaa aaccatctct aaaaggcttt tatcacagca
    17941 atttgaaaga tagtgtttgg caagtttatt acgaaaatgg caatcctcag ttagaagttt
    18001 tatacagaaa aggtttgagg ataaaaattt taaatgctta cgatttagaa ggtaagcaaa
    18061 tagtgaaaga tggcaacggg caaatgataa attattttaa tagtttaaaa attcaatctc
    18121 aagggaattt agaagatggg gtagagcagg gcttatggac tttttattat gagaatggag
    18181 taaaatcatc tgaaggcaat tttgaaaaag gtaagcgagt cggaaaatgg gaagaatggt
    18241 ttgagagtgg caagaaaaag agcgaattaa attacgaaag tggtttaaat atttattggt
    18301 ttgagtctgg caaaaaagag atggagggaa ctttaattga ttctaaacga gaaaaatcat
    18361 ggacatattg gtatccaagc gggcaagtta gaagtataaa taattatgta aatgatttaa
    18421 gagatggggt tcaaataaag tattatgaaa atggaaataa acaaagcgaa attaattaca
    18481 aaaatggatt gaaggagggc gaggctcact tttggtttga agatggtaaa aaagatattg
    18541 acggaaattt taaagatgat ttacaagacg gattgtggac atattggcga accgatggca
    18601 gtaaaggaaa tgaagggcat tttgtaagag gaaaaatgga aggaagatgg aatttttggt
    18661 atgctaacgg aagactgtgg aaagaagttg actatgctgc tggaataaaa gacggagttt
    18721 ggaatatata ttttgaaaac ggaaataaat ttagagaagg aaattttgaa aacgatttcg
    18781 aaaatggttt gtggaaagaa tggtacgata atggagtttt ggcaatgcaa ggaaattacg
    18841 acttaggtat tatggttggc aattggaaaa cttggcacga aaacggaagc aaaaaatcag
    18901 aagtggaata tacagatgga gttctttcag ggaaaaatta tttttggtat ccaaacggaa
    18961 aaaaacaagt agaagaaaat tataaaattg ttaaagaaaa atcttcgggt aaatttagag
    19021 aagaagaaag aataacttct gttttgcatg gttcttataa aacttttaat attagaggaa
    19081 ctgaaattat ttcaggagtt tattacgaag gactacgaaa tggaaagttt gtttataata
    19141 atgataatgg taatactgta aaaatagaat tttacaaaat ggataaacca gatggaaaat
    19201 ggcaaacttt ttatgatttt ggagcactag aatcagaagt taattataag aatggtgtta
    19261 aagaaggaaa gtcaacttat tacgaccaaa gaggaaatat tacttttcag tctatgtttc
    19321 gtggaggcag acaaaccgat gtaattgttg acgataatcc aaatttacca caaaataaag
    19381 gaggacaaaa tcaatataga agatgattag aattttatta agtggattgt tgattttttt
    19441 tgtattaaat ctttcttctc aagaaaataa aaatatgtat agcggaggta tgttgttttt
    19501 tcagcctggc tatgttagtg caagcaattc ttatcaaaaa ataagtaatt ttagcacagg
    19561 aattggtgga atgcttagat tttactttaa aaagcattta actttaggca ttcatggagg
    19621 aagtaataaa gcaatttata aatctaaaaa ctccgaaaat tcgtatatta gttttgctta
    19681 cggaggtcct tttgttggat ttacagccca aaaaaataaa ttaagatttt gctcttcgct
    19741 tggaattgga atggggaagc ttagaaattt acatatagaa agtcaaacag aaacagttct
    19801 aaacgagagt gagttatttt tttattcagg tgtttctttg aacccattgc ttagttttga
    19861 ttataatttt acagaaaaaa ttgcattaac tacccagcta atgacaatta ttactttata
    19921 taacaaaaat gatttgtatt tttgcccaac tttacaaata ggagttttat ttaatagata
    19981 aatataaaat atgaatagag acgagctttt taaaaaaatt attgcacact gtaaagagta
    20041 tggttttatt tttcaatcat ctgaattgta tgatggacta agtgcagttt atgattatgg
    20101 tcaaaatgga gtagaattaa aaaataatat taaagaatat tggtggaaag ctatgaccca
    20161 gcttaacgaa gaagttgttg gaattgatgc tgcaattttt atgcatccaa cagtttggaa
    20221 agcctcgggt catgtcgatg cttttaacga cccacttatt gataataaag attcaaaaaa
    20281 acgctatcgt gccgatgttt tgattgaaga acatattgag aaaattaatg acaaaataaa
    20341 aaaggaagta gaaaaagcag ctaaaagatt tggagatgct tttgatgagg ctcaatttgt
    20401 atcaactaat ccacgagtat taggatatgc tcaaaaaatt caagaaataa aatctaattt
    20461 tgtttcggct ttagaaaata acgaccttga aaaacttcgt caaattatta tagacgaggg
    20521 aattgtctgt cctatttctg gaagtagaaa ttggactgag gtgcgtcaat ttaatttgat
    20581 gttcgatacc aaattaggtt ctttaagcga aggagcaaat acagtttatt tacgacccga
    20641 aacagctcaa ggaatttttg taaattattt gaatgttcaa aaaacaggac gtatgaaaat
    20701 tccttttggg atagctcaaa taggaaaagc ttttagaaac gaaattgtag ctcgtcagtt
    20761 tatttttaga atgcgtgagt ttgaacaaat ggaaatgcaa tattttgtaa aacctggaga
    20821 tgaacttact tatttcgaag cttggaaaga aaaaagaatg aaatggcatc ttgctatggg
    20881 aattgcccta gaaaaatata gatggcacga acacgacaaa cttgctcatt atgcaaatgc
    20941 tgcttttgat atagaatttg attttccatt tggttttaaa gaattagaag gtattcattc
    21001 aagaacagat tttgatttaa aacaacacga agaattttcg ggtaaaaaaa ttagatattt
    21061 tgataacgaa actgatgaaa gctatatccc ttatgttgta gaaacttcta ttggtttgga
    21121 tagaacattt ttatcagttt tatctgctgc atataacgaa gaacaagttg gcaacgaaac
    21181 gcgtgtagtt ttaaatttac cgccagcttt agcaccagtg aaagttgcag ttttgccttt
    21241 agttaaaaaa gacggtttgc ctgaattggc tcgcaaaata atgaatgaac taaagtttga
    21301 tatgaattgt gaatacgaag acaaagactc tattggcaga cgttatcgcc gacaagatgc
    21361 tattggaact ccgttctgta ttacagttga ccatcaaagt ttagaagatg atactgttac
    21421 tattaggtac agagatagca tggaacaaga aagaatacct attaataaag tagctagcct
    21481 tgtaagagaa aaagttgata ttaaaaactt gttgaaacaa ttagtttaaa attatttagc
    21541 aagatttttt aaaaataatg aaaaatccaa aaccaatagt tttgtatgtc ttacttttaa
    21601 taagtatggc attgtggggt acttcatatg tttggggaaa aatggctttg gaggattatt
    21661 cggcttttac tgtaatattt ttcagattat tagtgtctgt tattgtttta ttaccagttt
    21721 tattcttgac taaaagattt aaacttccta acaaaaagaa tttacaccta tttataatac
    21781 ttgcattttt tgaaccattt ttatatttta tcggcgaaac taatggcttg aaatatgttg
    21841 atgcttcaat gacatcagcg ataattgcta ttatcccttt gtttacgcct tttgtggctt
    21901 attatttctt aaaagaaaaa atacgtgcag taaatatatt aggtattcta atttcaatat
    21961 taggaataat atttttggtt tttgataaac atatgaattt gcaagtacct ttaaaaggtt
    22021 tgctacttct ttctttagct gttgttgcag caaatggcta tagtgtaata ataaaaaaaa
    22081 tgcctttaga ccattctgta ttaaatattt tattttggca aaatcttttt gggctgataa
    22141 tgtttcttcc aattgttgcg atatttgata ttcaagcaat aaaagctaca ggctttgttt
    22201 acgacagtgc tatcgcaatt gtaaatttag gtatttttgc atcaacaata gcttttttat
    22261 tttttattta tgctttaaga tttatgccaa ttactaaagc taatgttttt agtaatacaa
    22321 ttccaatttt tactttaata atcgcttggc ttgtgatgga cgaaccactt agcattagaa
    22381 aaattattgg agtttttatt gtaattgctg gagttgtggt gtcgcagttg aggtataaaa
    22441 gttcttagtt tttagttttt agttaaactt taaaaacttt acgaacttta taaactttta
    22501 actcacttgt tgtgttcgtt taaattttca aaaaagttag aatcatattg tccaaacttg
    22561 tcttgaataa aatttagaat ttctaaaatt tcttcgctcg tttgtgcagt taaaagtttt
    22621 aatcttgttt ctttaaaatc tggaatagcc ttgaaataaa gagcaaaatg tcttctcatt
    22681 tcgtaaattc cttttttatc ttctttatat tcaagtgatt tttgcaaatg ttcttttgca
    22741 tattctactc tttctataac acctggactt ggcaaatgct ttcctgtatt taaaaaatgt
    22801 tttacttctg caaaaatcca cggtttgcca atagcagcac gaccaatcat aattgcatca
    22861 acgccgtatt tattaaattt atcgtaagca ttttgagcat ctgttatatc gccatttcca
    22921 attataggaa ttttaatatt ttgattttct tttacttttc caattaaaga ccagtcggct
    22981 tcgcctttgt acatttgtgt gcgagttcgc ccgtgtattg taagagcctg aatgccacag
    23041 tcttgtaaaa gcaaagcaac ttcttcaaca tttaaagaat ctgaatccca gcctaagcga
    23101 gtttttgcag ttacaggaaa ttttgaagtt ttaacaacta gttctgtaat ttctttcatc
    23161 agtggtaaat ttctcatcat tccagaacct gctcctctgt ttgcaatttt ttttacagga
    23221 cagccataat ttatatctaa taaatctgga ttagcatttt cgttaatcat taaacttgct
    23281 tgttgcattg cttctggaat atgtccgtag atttgtattc caacaggtct ttcgtaatcg
    23341 aaaatatcaa gtttttttac gcttttttta gcatctctaa ttaagccatc tgatgaaata
    23401 aattcggtaa acattaaatc tgccccaaat tttttgcaca tatacctaaa cgaagggtcg
    23461 gtaacgtctt ccattggtgc aagaaaaaca ggtttatcgc aaaattcgag ttcgcctatt
    23521 ttgagcattg attattttaa gattttatag agtttgttta acttcaattt catcgaatgc
    23581 ttcaacaata tctccaactt taatatcgtt aaatttgtca atatttaatc cacattctaa
    23641 gccggcagtt acttctttcg catcatcttt aaagcgtttt aagctaccta attcgccagt
    23701 ataaattaca ataccgtctc taataagtct aacttttgat tttctatgga tttttccgtc
    23761 tgtaaccata catcctgcga cagaacccac ttttgtaatt ttaaatactt cgagaacttc
    23821 gcaagagcca gtaacttttt ctttaattgt tggcgaaagc ataccttcca tagcatcttt
    23881 aatttcttct atagcatcgt aaattacaga atacaatcta atttctattt cttcttcttc
    23941 ggcaagtttg cgagcatgac tagaaggacg cacttggaaa ccaactatga tagcattaga
    24001 agctgtagct aacattacgt cagattcaga aatttgtcct actgctttat gtataatatt
    24061 aacagcaaac gagtctgtag aaagttttat taaagaatct gaaagtgctt caactgagcc
    24121 gtcaacgtca cctttaataa taagatttat ttcgttgaaa ttaccaagag ctaggcgacg
    24181 accaatttcg tcgagtgtaa tgtgtttttg agttctaagc ccttgttcgc gttgaagttg
    24241 taatcttttg ttagcaatat ttctagcttc gtggtcgctt tccataacat ggaatttatc
    24301 accggcttgc ggtgccccat ttaatccaag aactaacaca ggagtagaag gtccagcttc
    24361 ttcgactctg tttcctcttt cgttgtacat agctttaact cttcctgttt ggcaaccagc
    24421 aagcagaata tcgccaactt ttaatgttcc tgcttgtact aaaagagttg ttacatagcc
    24481 tcgtccttta tctaaactgg cttctattac cgtacctact gcatttttat taggatttgc
    24541 ttttaattca agaagttcag cttcgagaat tactttttct aataattcgt taatatttat
    24601 tcctgcttta gcagaaattt cttgagattg gtattttccg ccccagtctt caactaaata
    24661 attcatatta gctaaagctt ctttaatttt ttcggggttt gcagttggtt tatcaatttt
    24721 gttaattgca aaaaccattg gaactccagc agcacttgcg tggttgattg cttcaacagt
    24781 ttgaggcatc acgctatcgt cggcagcaat aatgataatt gcaacgtctg taatttgagc
    24841 accacgagca cgcatagctg taaacgcttc gtgtccaggt gtatctaaga aagtgatatg
    24901 tcttccgcta ggaagtttta ctgaatatgc accaatatgt tgagtgatac caccagcttc
    24961 gccatcaatt atgtttgtat ttctaattct atctaacaat tttgttttac cgtggtcaac
    25021 gtgtcccatt actgttacaa ttggtggacg aggaagtaaa tcttcttcag catcttcttc
    25081 ttcttcaatt tcactaagaa cttcagcact aacaaattca acttcaaatc cgtactcatc
    25141 ggcaactaaa gccatagttt cagcatctag tctctggttt atagaaacga ataagcctaa
    25201 gctcatgcaa gttgcaataa gcttgtttac aggtacgtcc atcattatcg acatttcgtt
    25261 taccgaaaca aattcggtaa cttttagtat gtttttttcg gcttcttgtt tttcaaattc
    25321 ttcttggcgt ttttgtgaaa ctaaatcacg tttttcgcgt ctgtattttg aagttcttga
    25381 tttttgttta ggtgtagtta aacgcgctaa agtttctttt atttgctttt gtacatcttc
    25441 ttggtcaata gatggtttag caggttttag attctttttg ttaggtcttc ttcttcttcc
    25501 ttcagaaggt tttgggtcgt catcaatatt aactttaact tttttaattc ttttgcgttt
    25561 agactttctt tcttcttttt ctttttctct tgttttttgt ctttcttcct tttcttgtct
    25621 tcttcttttt tcagattctt cttgacgagt taaaaatgag tctatatcaa tttttccaac
    25681 aacactagga ccagaaagtt tttcgatttt tgttttaata aattctgctt gtttagcttc
    25741 ttcgcttact gtaagagctt taaatactct tggtttttcg gcaggttttt ctttttcaac
    25801 ttttttcttg gtctctgttg tttcaggttg ttttttggtt tcaggcgttt tcttttcttc
    25861 agtagcttta gttttttctt ttttagcttc agcttctttt ttctttttag cagcttcttc
    25921 ttttagttta cgttcttctt ctttttgttt acgttcttct tctctttcgg ctttagtttt
    25981 tctttttggt ctagtttttt gatttatttg gtctagatct atagttccta caacttttgg
    26041 actggttgtg tttgctgtag tttcgctatt ttcaataggt ttaacttcgt gtttagctct
    26101 tgcaggtttt gttgtttttt cttctgccga gctttctgtt tgagttgagc ttttttcttc
    26161 actttcagta gtcgttactt gttcggcttc ggctattgtg gctttctcaa cagtttctag
    26221 ttctttttct tttgctaatt cactgatttt agcttgttta tcactagaaa gattcatttc
    26281 ttttgtttcg cgagcttgtt cagtgttttt aattgctttt tctttcaaag tcttagactc
    26341 cttaaattct tcttcaagaa gttcaacaca ttcaaaaggt aatttagagt tcggtccacc
    26401 ctctattgta taaccatttt tagccaaaaa ctcaataatt gtgcttgttc ccacattgag
    26461 ttctctggct aaagtactta gtcttaccgg ttttttgtct gaagtcataa actatattta
    26521 attactaaaa atttttattc aaattctgat tttaaaatat taagcacttc tataactgtc
    26581 tcttcttcta aatcagtacg tttcacaagg tcttctacgc ttaggtttaa aacgcttttt
    26641 gcggtgtcgc aacctattgc tttaaactgg tctataatcc attgttctat ttcatcacta
    26701 aattcatcta attcgacgtc gtcttcatct tcgtccattt ctctataaac gtctatagtc
    26761 attccaagta attggcttgc aagtttaata tttaatccac ctttaccaat tgctaaagat
    26821 acttggtctg gatttaggta aacatcaact ttattttctt cgggtttaat ttttatagat
    26881 gatatttttg ctggacttaa agctctttgt atgtatagct gagtgttggt agtataattt
    26941 attatgtcta tattttcgtt atgtaattct ctaacgattc cgtggatacg actacctttc
    27001 attccaacgc aagcacccac tgggtcaact ctttcgtcgt aagattctac cgcaactttt
    27061 gctctttctc cagggatacg aacaattttc tttattgtaa taagtccgtc aagaatttct
    27121 ggaatttcta attcaaataa tctttcaagg aatacaggtg atgttctaga aagtgtaata
    27181 agtggcgaac ctttttcaat tgaaaccgaa atcacaacgg ctctaatagt gtctcctttt
    27241 ctatatctat cgtttggaat ttgttcggct ttaggtaaaa taagttctat accttcgtca
    27301 tcgaggatta aaatttcttt tttccaaatt tgataaattt ctcctacaac aatttcgttg
    27361 attttgtctg tgtattttaa aaagatgtta tttttttctc tttccgcaat ttttgctttt
    27421 aaattttgtc ttagtgccat aatagctctt cgtccaaaat cttgaagttt tatttcgtca
    27481 gaaaactctt ctccagcttc ataatcttcg tcaatttttt gtgcttcgga tagcggaatt
    27541 tgtgtgtttt catcttcaaa atctacattc tcaactactg ttctagttct ccagatttga
    27601 acatctccat ttgcgtcgtt cacaataaca tcaaatttgc tttcttctcc aaaaagtttt
    27661 agaagcgtag aagtaaaaac atcttttaaa acacctatca ttgtaggacg gtcaatgttt
    27721 tttagttctt taaattctgc aaagttttct attaaattat tctgaagatc cataattttt
    27781 attttaaaat attatttact taaatgaaat tacacttttt gttgatttta tatctttaaa
    27841 tttaatttta gaaatttctg aaatttgttg ctttttgttt gccatttttg caaagtagga
    27901 ataacttata atgatttcgt catctcttat agttgttaat gtcccagtaa ttttttctcc
    27961 agtttttaaa ataacttcta cttctttatt taggcttttt tggtattgtt ctggcacctt
    28021 aaaaggattt gataagccag gcgaactaac ttcaatagaa aaatcttctt tatctctatc
    28081 taaattttct tctataaatc tacttatcct tctgcattct tcaatattta gtccattaaa
    28141 atcgtctata gtaacaaaaa tatcgttatt gttactaatt ttaatttcta ccaaaaactg
    28201 agtttcagtt agtgcagatt tacaaagatt ctctatttct tttttgtcta tcatcaataa
    28261 tttttaataa aataggggac ttaatgtgcc ccctaatctc atcgtttaac ccaaaaagcg
    28321 ttgcaaaaat actatattat tttttaatta ccaaaaaaac attgatttgt tttagcatat
    28381 gtttaaaata ttttttaata ttgtttagtt ttatattttt gtaaatgaaa ttttaattaa
    28441 aaaattatgg attttttgtt tattacttgg aatgttgatc cagttatatt ttatcttggc
    28501 aacagaggta taagatggta cggcttattg ttagctatag gttttttagt tggttatttg
    28561 cttctatcaa ggcagatgaa aaaagaagga gtgcagcaat tagttgttga taaacttgca
    28621 atttatgtta ttgttggagt tgttttagga ttgagattag gtcattgttt gttttataat
    28681 cctgggtatt atttaagtaa tcctatagaa attttatatg tttgggaagg aggtttagct
    28741 agtcatggtg gagctgctgg aattttgtta gcggtgtggc tattctcccg aaaatttaaa
    28801 attaattatt tgagtttgct tgacagagtt gttttaacag ttccttttgc aggaggaatg
    28861 gtaagacttg gtaatttaat gaattcagaa ataataggag tgcaaacttc tatgccttgg
    28921 ggttttaaat ttttgagaaa caccgaagac cttattcctg caattaaagc ttcgggagga
    28981 aagtgtggtt ttgaagattt ggcttgtttg gcagagttct ggactcctcg acatcccact
    29041 caattgtatg aggcaatttt ttattttata atgtttgtag ctttttattt tatttatcaa
    29101 aaatatattc aaaagtggaa acctggagtg tttttaggtt ggtttattac agtgttgttt
    29161 gtttttagat attttattga atttacaaaa gttgatcctg ttgaatttga tggttggaca
    29221 gatgctatta gaatgggaca attgttgagc ataccttttg tgctgcttgg attattctta
    29281 atgggacgag gttatggttg gtttaaaaga aaagctaaag ttaaaaaaga ataaatatta
    29341 ttgatatgag agattttgaa ttttataatc ctgtaaaaat aatttttgga aaaaataaga
    29401 ttgctaatat tgctgaagag ctaaagcctt ataataaaat catgtttact tatggtaagg
    29461 gcagtataaa aagaaatgga atttacgaaa gggttgtttc tgctttagag ggtaaagatt
    29521 ttatagaatt tggaggaata gaacctaatc cttcttatga aacactgatg aaagccgtaa
    29581 aattagggaa agaaaatgga gttgattttt tattggctgt tggtggaggc tctgtaattg
    29641 atggaacaaa atttattgca gcagctataa aatattcagg tgatgaccca tgggatatga
    29701 ttgcaaaggg tgaaaaagta ttagatgctg tgcctttggg tagtgtttta acaatacctg
    29761 caacagg
//
LOCUS       DAOPNX010000022        29275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_2051_length_29275_cov_6.814134, whole genome shotgun sequence.
ACCESSION   DAOPNX010000022 DAOPNX010000000
VERSION     DAOPNX010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 29275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29275
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2051_length_29275_cov_6.814134"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3922
                     /locus_tag="PKN53_03875"
     CDS             <1..3922
                     /locus_tag="PKN53_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HOD88123.1"
                     /translation="SPYAPPASGTGNFVLEISTDFGNTWSEIETVSNNGVAGWQTKAY
                     DLSTYVNTGDFLKFRITANWISGDYCIGFDNFNLASCLPVSDLSASNITNASATISWT
                     ASGSNPDDYQVYVSLANIAPAEDETNIEISNTNSIDLTGLEDSPLHYVWVRSDCGAGD
                     ISEWVGPLALWTSCEPIVALPYTENFDAYGTGTTAFPNCWSKPETYNSYPYIVDAYSV
                     SSPASLRFNSSTTTPTYAVSPAFAEDIQNLRVSFQLRAESTTYSGTIELGVMSDPNDI
                     STFESVATIQPTNTSFNYYYYDLYLATLSGANNYIALRHNATSTSYYYWLDDFKVELS
                     PSCLEPANFEASNINTTSVSLSWTALPTAPENGYDIYVSETETAPTELSAPTHNTAEA
                     LINIEGLTSATVYYAWLRANCNNSDVGEWTGPITFTTLCPPIATLSYTENFDSYSVGN
                     FPDCWLRPVTYNGFPSIVSGSSYSVSSPNGLKFQSSTTTPTYAVSPAFAEDIHNLRVS
                     FQLKAESTTYSGTIELGVMSDPNDISTFESVAIIQPENTSFNAYQYDLYSTTLSGANN
                     HIALRHNSNSSSWYYWLDDFEVDLSPTCIEPNGFAVNSVTANSASISWTESITLPENG
                     YDIYFSTTATAPTGSSTPTHNTNETSFLFENLDESTSYYVWVRANCNNADQSDWTGPL
                     HIYTGYCIPTNTSTSYWISSLTTTGGITNISHTGITSQPGGYGDFSAIHSCSNVEGQP
                     TMINFAQNTGTGYYFCWIDWNKDMQFDATERIIAATSSYSSTYQGTINIPAGTEPGEY
                     RMRVANSYLYEITDACTGSGYGQYFDYTFVVVEPANNETDILTYSFAEQIAPATIDNV
                     EHKVYVTVAAGTNLNGLVATFTLSEGASATVAGLPQQSGVTPNNFTYPVVYKVVAENG
                     LDEQPWQVFVDVDDTGLSSEADILAYTIPNQVGLTSINSTNRTVALTVPYGTDLTALV
                     ATFTLSNGATAEVETVTQQSGVTPNNFTNPVTYTVTAENGTTTKNWTVTVTEAPASDQ
                     ADILSYVIPNQVGLTSINSTNKTVALTVPYGTDVTALVATFTLSNGATAEVETVTQQS
                     GVTPNNFTNPVTYTVTAQNGTTTKDWIVTVTVAGEPLSSEADILTFSFAEQFEEAIIN
                     NVNRTVNIKVNEGTNLTNLVATFTLSEGAIAKVAGLVQQSGVTPNNFTNIVIYSVTAE
                     DGTTVKNWAVNVSEHSGIEDIEISKLSIYPNPNNGRFTLDFSDIYGKVNYQIYDTKGS
                     IILSDNLVVNGNAMKEVSLNIAPGVYYVKLITETQSIIEKLVVE"
     gene            4175..7225
                     /locus_tag="PKN53_03880"
     CDS             4175..7225
                     /locus_tag="PKN53_03880"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOD88124.1"
                     /translation="MRKILTRLTLILVFCIGVSAFSKLQSQIVVAKWDFGNTTKQDAI
                     TDNASFASSPYTADDGIVENKDIATISLGGSVNYNGWVTGASGTPDKAPNSTAWNLTD
                     GYWQIGIVTTGYKDLTLSSKQQSSNTGPTDFQIQYSTDNGSSWLDVGTAITVANNFTS
                     GVVTDLALPAECNNLANLLIRWTNTSNVSVGGTTVASGGTNRIDDIVVMGTLDDPCLA
                     TVPFFEGFENGYNHDDVVEGCWTQESETGTATWVVNSTNTTYNRTPRTGLFNATLQYS
                     NKDWLFYKVYLEQGESYDFTVWARQDATSGATLTLAWGTEDNSAAMTNIIVDAQVITN
                     GDYQEVKGTFGVPTDGVYYIGIKGSLTYTPYYMSIDDISLDFGPNCVEPTAIVASEIT
                     ANSANISWTASVSAPASYHVYYSTTNTAPADDIADAEYAEVTGLTTELSELTPVTTYY
                     VWVRSACGADENSVWTGISFETTPSCFEPTNIQASDITINSANISWTAATESPDATYH
                     VYYSLVDAAPADDIDIAECEAPATNSIELTDLTSSSTYYVWVRSVCGADDFSTWAGPS
                     VFSTSCAIISEYPYFEGFNSGIDPACWTIIDNDNSGDSWYSGTYASTPSYEGASMMVS
                     EFSEPNDDWLISPQFKITSDNLKVDFYAKSYSAYFYESFNVLISKTGTSPSDFTIVLE
                     NIVDHPNTWQNHEYILSEHGINIDDEIYVAIQHVSNDQFVLFIDAFTVSEIEIPSAEA
                     EILTYSIPNQVGDTEITSTETEGTITVTMPYDTEVANLVANFTLSYGATAKVGAIDQE
                     TGVTPNNFTDPVVYVVTAEDGTTTKNWTVTVNLEAAPSSEANILTYSFEEQYSPAVID
                     DVNHTVTVVVNEGTSLDALVATFTLSPGASAKIGAVDQESGVTENNFTDPVIYTVTAE
                     DGTTTKDWTVTVSVYVGIEDVENSALAIYPNPNNGRFTLDFSDIYGKVNYQIYDTKGS
                     IILSDNLVVNGNAMKEVSLNIAPGVYYVKLITETQSLIEKLVVE"
     gene            7354..7650
                     /locus_tag="PKN53_03885"
     CDS             7354..7650
                     /locus_tag="PKN53_03885"
                     /inference="COORDINATES: protein motif:HMM:NF021988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOD88125.1"
                     /translation="MTIQELNKAFELAYGKPAEAIYFAPGRVNLIGEHTDYNGGPVFP
                     CALSFGTKLLIAKNNKKVMNFKSLNLPEVESLKFDQLTTRLENSWVNYPLGVMA"
     gene            7654..8514
                     /gene="galK"
                     /locus_tag="PKN53_03890"
     CDS             7654..8514
                     /gene="galK"
                     /locus_tag="PKN53_03890"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOD88126.1"
                     /translation="MKRGKTFTHGYDILIWGNVPNGAGLSSSAALEVVTAFALNDQLK
                     AGFSREEIALIGQKAEHEFALVNCGIMDQFVSAHGAKDHAVYLDTTTLEYELVPVILE
                     GVKIVISNTHSPHKLDSGAYNQRVAECKKAVEQLNNVRPIKFLAELTEADFKKIESAI
                     TDPVAHKRARHVVGEVQRTADAVKALKAGDLKLFGELMNASHVSLRDDYEVTGPELDC
                     MAEEAWKIDGVIGSRMTGGGFGGCTVSLVKDEAIDTFIKEVGANYEKRIGIKPDFYIA
                     EIGDGATRLV"
     gene            8534..9538
                     /locus_tag="PKN53_03895"
     CDS             8534..9538
                     /locus_tag="PKN53_03895"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOD88127.1"
                     /translation="MKNINTYTIKNKNGMTAELTNYGTKITRLLVIDKYGKFDDIVQG
                     FDTLQENIDKEPYFGATCGRFANRIKNGQFTLDSVKYTLAQNNGGNALHGGIVGFNAK
                     VWDVIKQTDNSISMQYISPDGEEGYPGTLITVVKFTISEANELQIEYKATTDKVTIIG
                     LTNHSYFNLKGAGNGTVRNHILQVNADFHTVLDDTACPTGEIRPVDNSPFDFRKPTVI
                     EKYIDAEEYLPGRGIDNNWAIRKNQPKELALAATLYEPTSGRVMEVLTTQPGVQVYTA
                     NWVEKQIGKYGKTYAKQNSICIETQNFPNSPNIAHFPSSILRPGEIYEEMCIYKFSVK
                     "
     gene            9649..10296
                     /locus_tag="PKN53_03900"
     CDS             9649..10296
                     /locus_tag="PKN53_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007441850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOD88128.1"
                     /translation="MNKNTNKEPRVKGLGCLGLTVFVVPIILILVAFLIWAGYYAYSS
                     YNKFIDADQMVKNSWANVENTYQKRYDLIPNLVETVKGYATHERETFTAVTEARAKAS
                     SMNISAEELTEENLRAFEDAQSELSGALSRLLVSVEAYPELQANQNFMQLQSDLKEIE
                     NEILQRRDEYNNTVKPYNNLVLKFPRNLFAKMFGFKEKPYFKANEKASESPKVSF"
     gene            complement(10293..10997)
                     /locus_tag="PKN53_03905"
     CDS             complement(10293..10997)
                     /locus_tag="PKN53_03905"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOD88129.1"
                     /translation="MKDIFHFKKFSLKQSSSLLKVGTDAVLLGAYIDCKDAKHILDIG
                     TGTGIIALALAQKSEAIIHALDINSEACKLANENFQNSNWSNRLTIFCQAVQNFETDI
                     KYDIIVSNPPYFSAGNASPNKGKAIAKHDNELKLSDLLLNIKRLLNKNGLAYLIFPAL
                     QHDSFIQECENAELFVSKKLFIIPKKGKQANRIIFELSHQFSICENSHLTIENDFRHD
                     YSEEYKELTKDYYLKF"
     gene            complement(11001..11768)
                     /locus_tag="PKN53_03910"
     CDS             complement(11001..11768)
                     /locus_tag="PKN53_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOD88130.1"
                     /translation="MNTKLDFSNLKEFLDEKHDKFNNPNFIENDPIQIPHSFNKKEDI
                     EISGFLAATIAWGKRSTIIASSKKMMNLMGNNPYEFVMSHSKNDLDNFPTNIHRTFNN
                     EDFKFFITSLKNIYENHGGLENVLTKTDNIFDNLKDFHEIFFSIPHLQRTKPHLANVA
                     SGSAAKRLNMFLRWMVRNDKRGVDFGIWKNISPACLYIPLDVHSGRIARLLGLLERNQ
                     NDWRAVDLLTQKLREFDHKDPIKYDFALFGIGVNEDF"
     gene            11838..12476
                     /locus_tag="PKN53_03915"
     CDS             11838..12476
                     /locus_tag="PKN53_03915"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOD88131.1"
                     /translation="MILTNLWKGIIVGLSASIPLGPIGVICIQRTLNKGRRSGFISGL
                     GAAVADGLYAFIAGFGVKIVMDSINEYQIFIRSIGGVFLLFMSYKLYVTNPGVQLRKQ
                     LKKKRSGLLGDFFSVFALTISNPITLFVFAVVFAGFNVVDKTSDLIVVILLLIGVLLG
                     AVLWWFTLTSIVNKFRGKFKLRRMLYINRVAAILIALFAISIIVSIFFDKSI"
     gene            complement(12510..13253)
                     /locus_tag="PKN53_03920"
     CDS             complement(12510..13253)
                     /locus_tag="PKN53_03920"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HOD88132.1"
                     /translation="MKKILEICCDTIKSVKNANNAKAQRIELCTALNTGGISPSYSFL
                     KQAIEISDIPINALLRPRAGNFVYSDIEFEIMCEELKEMKRAGVAGIVSGVLLENGRI
                     DKERTKKLLELSKPQSFTFHRGIDFCTDIDEAMDFLCEIGAKAVLTSGGCAKINEGLE
                     NIIIMHKKYGNKICIMPGGGLNFENAKILKDVGIYEFHFSASEFEEESVVYSRYPNGL
                     GHIINGEKYGYNYSSKNTIKKMLEVLKKD"
     gene            13316..15124
                     /locus_tag="PKN53_03925"
     CDS             13316..15124
                     /locus_tag="PKN53_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HOD88133.1"
                     /translation="MRTIFYSIFLILISTNIMAQEERFSFPDQLKCRFMPKHAKDTAV
                     FFANKGIWHGYTLPENNDTKNCGKFGGPYCFYTKKWFAPSLLDLSLNVAGQGKIDLNT
                     AKTKNLNQFPGLLMQEFEFDDYKLILQLEAVTTRASLYQAILINTSDEAKKFSMQLEG
                     NIFDNTSKIEQIPDGWIYKVDGQEDIIWFVRFRLDAQMELNYSDTNYEFKYKAMQTVQ
                     ANDSLKITAVVAQFFNGDLQQDVLITNDILSTPQLYVDNNKNFWNFLFGHMTTQSNIR
                     RQMTLSAFQTIYLNSRSALPTKKYLTVLPYAETKDLFINTDEAWFTAVSLLKFDPRLS
                     YNIVSFILNSINKDGALKKYLSLVPKIDDKGEIIDKPMAAWTVFNIYSVTQDDEFVKM
                     CLPFLDKYLSYWFKDRDVDKNGWVENSQGIEEVALNAILFTETHSMLKMYEAIDDEEK
                     IEYYQKQVARIADNFNLYFFQVDHNRYCDFNAETQEYTVAENTIGYCLWSGIANQSIA
                     DVYAQEIAKDINSDEIFYRLANSDIDPGYLYLMAFGFKLFNYAQIQEALKSIYTNSVD
                     FEFMNNNKLFVKKYDSKTVEYSTYMAAVYLLMANYF"
     gene            15291..16361
                     /gene="serC"
                     /locus_tag="PKN53_03930"
     CDS             15291..16361
                     /gene="serC"
                     /locus_tag="PKN53_03930"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOD88134.1"
                     /translation="MRKHIFNAGPCKLPDRTLENTAKAILEFNNSGQSIMEVSHRGKD
                     FVAVMDNTIELMREILNIPENYSVLFLGGGASLQFAMIPYNFMKSKAFYLDTGTWSTA
                     AIKEAKLFGEVIDLSSKDKAFTYIPKNFKVPSDVDYVHITTNNTIRGTEIHEDLTFDK
                     PLFADMSSDILSRPVDVSKYAMIYGGAQKNMGPAGVTFVILRNDMLDKVVDRPIPTML
                     KYSTHIDKGSMHNTPPVVNIYALRETLLWVKEQGGVKAMDKLSNEKATMLYNAIDESK
                     LFTCPVVKEDRSRMNVIFVLEEKYKHLEEDFVKFATSKNCVGIKGHRSVGGFRASIYN
                     ACTVEDVKALVEVIKEFDKQHA"
     gene            16526..17488
                     /locus_tag="PKN53_03935"
     CDS             16526..17488
                     /locus_tag="PKN53_03935"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOD88135.1"
                     /translation="MFLKIVLLIAVIFQIIAVIVSLRLTRKTKYNLSWIFISLGFGAL
                     LMRMILEALPFYFNLEPKAYGYLYMWLGVASAMFFAIGLILIRSIFTYMEKIEKEKRA
                     SEKRFLSVLIQAEDNERRRLAKDLHDCIGPLLSTIKMSLSALKKLNTDEQQKVILDNL
                     DTVVVESMKSVKEISNNLNPHVLDNFGLDKAIANFIPKITTTGDLKIKYNSSLQDLRI
                     ETSKETVLYRVVCELINNTIKHANATQIDIDLYYDTRKIYLTYKDNGSGFELDNLFKH
                     QDKGTGIYNIYNRINSLKGTVNIDSKLDCGTKVEIKIPYSNENN"
     gene            17475..18137
                     /locus_tag="PKN53_03940"
     CDS             17475..18137
                     /locus_tag="PKN53_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOD88136.1"
                     /translation="MKTIKKTYSLMIVDDHALFRNGLKLILSSLPNFKVVAEASNGEE
                     FLNTLDLIPVDIVLMDIEMPVLNGIEATKLALKKQPNLKIITLSMYGDEEYYHKMIDA
                     GASGFLLKDSDIDEVHSALNSVAEGKSYFSQELLQNLIKNFQNQDENPKETFDLSARE
                     VEIIDLICQGFSNIEIGEKLFLSKRTVEKHRANILEKTNTKNTASLVMFAIKNKIVDV
                     EQ"
     gene            18204..18761
                     /locus_tag="PKN53_03945"
     CDS             18204..18761
                     /locus_tag="PKN53_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88137.1"
                     /translation="MFILFLLQSNLYSQVVKYKSDLPVVYYRDSLQKAGEYKLSLQNH
                     LSSLSKEFTCEALYKIAVNYAELNLKDSALYYLNLYVDHSSDDRLILADKAFEPLKDK
                     KADWHKLEIKIKNSYLKSLPDNINENLALELFELSILQNLYEYYIDLLKMPNSAFETK
                     NEMESSMFSNPRLKKKLIQNKYCLT"
     gene            18761..19165
                     /locus_tag="PKN53_03950"
     CDS             18761..19165
                     /locus_tag="PKN53_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88138.1"
                     /translation="MKSENIFKAILQKYGFPSVEKAGVFGENIAYKSIMKLNVSEQYL
                     DSVENLFNKKLFDPLAYAMIKDKYLYMKDKKQEFGTLLYYCENDSKWELSLYPVSDFK
                     NLNQRRKEIGILETVEDYANRRDAKIPKSYYK"
     gene            19246..19614
                     /locus_tag="PKN53_03955"
     CDS             19246..19614
                     /locus_tag="PKN53_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88139.1"
                     /translation="MRKISFLVFGLACIILLSSCGGPKTDAKKLETLLKAHTQAFVEI
                     ASDNKIDEKEAKEVSKLMEEMRNFNSEIEKKYESDPKGKEMLEEYFNKNEENFSLIYT
                     DYYNSLFGLFNCEGSENLDL"
     gene            complement(19620..20495)
                     /locus_tag="PKN53_03960"
     CDS             complement(19620..20495)
                     /locus_tag="PKN53_03960"
                     /inference="COORDINATES: protein motif:HMM:NF039704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6252 family protein"
                     /protein_id="HOD88140.1"
                     /translation="MKQIIFLSVIIFVFAFFCSCGNSGGDNSAKPAMFCLVNSTNWRS
                     PEPKAILTETSIKVFGTSANGQTIILNVGSPQKGEYTANAFTGCYAEFIPNMSAGAAR
                     YSTLNSESAYGTIKIESINEESKTMSGSFSFKAYRSTDNTFRTINDGVFTNVPYHFYS
                     NSDTTYQNIFYYSTEGRTWYAKDITAQKNDTALVIFADCDRTETWQSVTMWMSPTIGS
                     GVHYITADGPIYAKFQKGFSTYDATNGSVTIVENNSTTKKFRGTFFFNYKNENDETIA
                     ISDGAFDIKFTEESE"
     gene            complement(20590..21555)
                     /locus_tag="PKN53_03965"
     CDS             complement(20590..21555)
                     /locus_tag="PKN53_03965"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOD88141.1"
                     /translation="MKKSSLKNHILFLVKLIIVALILLFAYFKLKNSSDFPELLKLLF
                     EFNSTQISYLILLIILMLANWLLESEKWRFIISKIEKLKPLTALSFVWTGVSIGTLTP
                     NRIGEFVGRVMFLKPENRKRASSLTLIGDLSQLLITIFFGSLAILLLIPYFDKDSNLI
                     FNKSIVYTFIILSFTLSFGVFFSINKIINFFKNRNFKPNWFSKFYDLPIISFKDKLIL
                     FSLSLLRYLVFSFQYFVALKIFGIDISFYNCFIASSAMFFASHLLPNIAIVEVGIKIS
                     FSILFFGLFTNKISAISIASLLIYIVNILIPIVIGGINILYRRKK"
     gene            complement(21559..22548)
                     /locus_tag="PKN53_03970"
     CDS             complement(21559..22548)
                     /locus_tag="PKN53_03970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOD88142.1"
                     /translation="MKTTIKNIIKFIIFIGIGAVLFLLVYKDFDFVELVEEAKTLDYK
                     WFLLMAVLGLFSHFFRTFRWKMLLETEGNKTRFSNTFLAVANGYFANLALPRLGEVTR
                     CGIISKYEKIPFAKVLGTMISERAIDLIIFFIFAVIGVIVGYDQVLEFLRKNPKFGDN
                     LEFLFSWKFIVLSTIIIAIGIFFIVKIAKGKYNKYKIFAKISNFLNQLWEGLISIKNI
                     NNKAKFIFHSFFIWILYFLMLYSCFFAFDSMQHLDLSVALVIFVAGSLGMLAPAPNGI
                     GAYHFMVIQTLIIYGIAESSAASFALITHGIQTVLLILLGVLSFILLPILNKK"
     gene            22699..23439
                     /gene="rsmA"
                     /locus_tag="PKN53_03975"
                     /pseudo
     CDS             22699..23439
                     /gene="rsmA"
                     /locus_tag="PKN53_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760928.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
     gene            23464..24276
                     /locus_tag="PKN53_03980"
     CDS             23464..24276
                     /locus_tag="PKN53_03980"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOD88143.1"
                     /translation="MFVSDFDGTIANNSSVVSENTINLLKKLEEISVLRVIATGRSLF
                     SFTTVVKQDFPIDYLVFSSGIGIYDWKTKKIIQSSCIGSEQTKKVYDYLVENNYDFMV
                     QLPVPDNHFFHHFSSDCPSSDFLTRVKFYETKGLEPISECPETASQFVVICSEENGQM
                     EKISKKFPDLKVVKATSPLDNKSIWIEILPYQISKASGIEFIRQLENIEHENIVTVGN
                     DYYDLDMLNYSLKENSYLVENSPDEIKDNFNIIASNENEGVAKLLQRIYSVN"
     gene            complement(24647..26011)
                     /gene="hisS"
                     /locus_tag="PKN53_03985"
     CDS             complement(24647..26011)
                     /gene="hisS"
                     /locus_tag="PKN53_03985"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019968875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOD88144.1"
                     /translation="MAQPTSIPKGTRDFGTLEMMRRNYIFETIKSVFKKYGYSPIETP
                     AMENLSTLLGKYGDEGDKLLFKILNSGNFSKNVNADDLGEENLNKLSSKISDKGLRYD
                     LTVPFARFVVQHRNEIQFPFKRYQIQAVWRADRPQKGRYREFYQCDVDVIGSDSMLNE
                     FELMQIIDEVFEKLDIAVVLKINNRKILEGIAEVVGAPDKFMEITIAIDKIDKIGLEN
                     VCKELKDNGLSDEAINKLIPILENKRNSSETIEFLESLLQNSEAGLKGIEEIKSIFDY
                     LKISPLDTEVELDISLARGLNYYTGAIFEVKAKDVEIGSILGGGRYDNLTGIFGLKDV
                     SGVGISFGADRIYDVLASLDKFPETINSPVEVLFVNFGSEEEKLAIKLAKKLREDGVS
                     CEIYPDNAKLDKQFKYANNKNINFVVIIGANEIKNETVSIKNMKTGEQKSVAVEKFNT
                     TIFK"
     gene            complement(26017..27114)
                     /gene="ychF"
                     /locus_tag="PKN53_03990"
     CDS             complement(26017..27114)
                     /gene="ychF"
                     /locus_tag="PKN53_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOD88145.1"
                     /translation="MALHCGIIGIANCGKTTVFNCMSNTKAETTSFAFASNKSNIGVI
                     NVPDTRLKELEKFQKTEKIIPATVDIVDIPGLTKGSSSGEGVGNQFLADLRNADAIIH
                     VLRCFEDENLPHIEGKVDPVRDIETIDLELQVKDLESITKKIARLEKLIKVGDKDAKA
                     SIEILKIYQEHLENMQNARTAPVSEEDRKHVAELSLLTEKPVMFVCNIDEDSIAEGNK
                     HVESVKEYLKDQNVKILTVAAKLESEIAELETDEERQEFLNDYGLSEPSVNKIIREAY
                     DLLNLQSFFTVGPKEIRAWTITKGMTAPEAAGVIHSDLQRGFIRAEVMKYDDFVSLGS
                     ELAVKNAGKFHVEGKTYVVEDGDILHIRFNV"
     gene            27280..28089
                     /locus_tag="PKN53_03995"
     CDS             27280..28089
                     /locus_tag="PKN53_03995"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOD88146.1"
                     /translation="MKTKTSVIIIALLGLFCLKANAQIIKIADKIFVAGYVFEENTKE
                     PIPYVNIYVKQTRRGTITDNKGYFMLKAEINDTLIFSCLGFDKKYVHINQEVIDNNEA
                     LIISLDTKIYEIQSVDIYALRKYKQLEYEIKSMKLKDDDIVHAQSNFPLRPVDVDYYD
                     LVQSAPLSGFGVVISPITALYEACSKKGKENRKLAELKKQDNIQNLIEQKLGVENIMK
                     IINEDEATTLDFIEWCDFHEDFLLNISAYDLATVIKYKYKVYKVHPSLRAR"
     gene            28231..29220
                     /locus_tag="PKN53_04000"
     CDS             28231..29220
                     /locus_tag="PKN53_04000"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOD88147.1"
                     /translation="MYILKSESTDPYFNIALEEYLLSNYKEDFFIVAVNEPSIIVGVN
                     QNTNDTINALYVKVNKLKVVRRLSGGSAVFQDEGNLDFSNIYTDSSATLIDFTRITEL
                     IIEFLKTKFNIEANASGRNNILIDGKKISGQARARFGEKILNHGTLLLSSNKKSLVDA
                     LKFNEEKYKDQKLKSNLERITNINEHIEGSITMDKFIDLFLNFIQSKFPQAQRFNLSH
                     DDINAVNELVNLKYSNWDWNYGNIPEYNINTSLNTVDGFVSMYLNVVEDKIKNIKIYG
                     DFFSQLNISDLENVLVGVKYEKSEIEKALANLDFNCYMKGIDKQDFISHLFKN"
BASE COUNT        10501 a         4320 c         4676 g         9778 t
ORIGIN      
        1 ttccccttat gcaccaccag cttctggtac tggaaatttt gtattagaaa tttcaacaga
       61 ctttggaaat acatggtcag aaattgaaac tgttagtaat aacggtgtcg caggttggca
      121 aactaaagca tatgacttat caacttatgt taatactggt gacttcctta aattccgaat
      181 tactgcaaat tggattagcg gagattattg tataggattt gataatttta atttagcatc
      241 ttgtttgcca gtaagcgatt taagtgcaag caatatcaca aatgcttctg caactatttc
      301 ttggactgca tctggttcaa atccagatga ttatcaggta tatgtttctt tagcaaacat
      361 tgcaccagca gaagatgaaa ctaatattga aatttcaaat actaattcaa ttgatttaac
      421 tggattagaa gatagtcctc tgcattatgt gtgggtaaga tctgattgtg gagctggaga
      481 tattagcgaa tgggtaggac cacttgcctt atggacaagt tgtgaaccaa ttgtagcact
      541 cccatataca gaaaattttg acgcttatgg aacaggaaca actgcattcc caaattgctg
      601 gtcaaaaccc gaaacatata atagttatcc ttatatcgtt gatgcttatt ctgtaagttc
      661 gccagctagc ttaagattta actcttcaac cacaacgcca acttatgccg tgtctcctgc
      721 atttgcagaa gatattcaaa atttaagagt aagtttccaa ctaagagctg aaagcacaac
      781 atattctggt acaatagagc ttggagttat gagtgatcct aatgatataa gcacttttga
      841 atccgtagca actattcaac ctacaaatac tagctttaat tattattact atgacttata
      901 tttagcaaca ttgtcgggtg caaataatta tattgcatta agacataacg ctacttcaac
      961 tagttattat tattggttag atgactttaa agttgaactt agtcctagtt gtttagaacc
     1021 tgctaatttt gaagcaagca acattaatac aacttcagta agtctgtcat ggacagcatt
     1081 gcctacagca cctgaaaatg gatatgatat atatgtatca gaaacagaaa cagctccaac
     1141 agaattgtct gctccaactc ataatactgc tgaagcgttg attaatattg aaggtttaac
     1201 gtcagcaact gtttattatg cttggttaag agcaaattgt aataatagtg atgttggaga
     1261 atggacaggc cctattacat ttactacttt atgtcctcca atagctacac tttcatatac
     1321 tgagaacttt gacagttatt ctgtaggtaa tttcccagac tgttggttaa gacctgtaac
     1381 atataatgga ttcccatcta tagttagtgg tagtagctat tctgtaagtt caccaaatgg
     1441 tctgaaattc caatcttcaa ccacaacgcc aacttatgcc gtgtctccag catttgcaga
     1501 agatattcat aatctaagag ttagcttcca actaaaagct gaaagcacaa catattctgg
     1561 tacaatagag cttggagtta tgagtgatcc taatgatata agcacttttg aatcagtagc
     1621 aattattcaa cctgaaaata ctagttttaa tgcatatcaa tatgacttat actcaacaac
     1681 tttatcgggt gcaaataatc atattgcatt aagacataat tcaaattcat caagttggta
     1741 ttactggtta gatgattttg aagttgatct tagccctact tgcattgaac ctaacggttt
     1801 tgcggtaaat agtgttacag caaattcggc tagtatatca tggacagaat caataactct
     1861 acctgaaaat ggatatgata tttatttctc aacaacagct acagctccta cgggaagctc
     1921 tactccgaca cataacacta atgaaacttc attcttattc gaaaacttag atgaatcaac
     1981 aagttattat gtttgggtac gtgcaaattg taataatgct gatcaaagtg attggacagg
     2041 tccattacat atatatacag gttattgtat cccaactaat actagtactt catattggat
     2101 atcttctcta acaacaacag gtggtattac caatatatct catactggta ttacttctca
     2161 gcctggtggt tatggagact tctcagcaat tcattcttgc tcaaatgttg aaggtcaacc
     2221 aactatgata aattttgcac aaaatactgg tactggttat tatttctgtt ggatagactg
     2281 gaataaggat atgcaatttg atgcaacaga acgcataatc gcagcaacat caagttattc
     2341 aagtacatat caaggaacta taaatattcc agcaggtact gagccaggag aatatagaat
     2401 gagagtggca aatagctatc tttatgaaat tacagatgct tgtactggaa gtggatatgg
     2461 acaatacttt gactatacct ttgttgtagt tgaaccagca aataacgaaa cagatatatt
     2521 aacttattcg tttgcagaac aaatagctcc tgcaactata gataatgttg aacataaagt
     2581 atatgttact gttgcagcag gaactaactt aaatggacta gttgctacat ttactttatc
     2641 cgaaggtgct tcagcaactg tagcgggatt acctcaacaa agtggtgtta caccaaataa
     2701 ctttacatat cctgttgttt ataaagtagt tgctgaaaat ggtcttgacg agcaaccatg
     2761 gcaagttttt gttgatgtag atgatacagg tttaagttct gaagcagata ttttagctta
     2821 tacaattcca aatcaagttg gactaactag cattaattca acaaatagaa cagtagctct
     2881 tacagttcct tatggaacag atctaactgc tcttgtagct acatttactt tatctaatgg
     2941 tgctactgca gaagttgaaa cagttactca acaaagtggc gttacaccaa ataatttcac
     3001 aaatccagtt acctatactg taactgcaga aaatggaaca actactaaaa attggacagt
     3061 tactgttacc gaagcaccag ctagcgatca agcagatatc ttatcttatg ttattccaaa
     3121 tcaagttgga ctaactagca ttaattcaac aaataaaaca gtagctctta cagttcctta
     3181 tggaacagat gttactgctc ttgtagcaac atttacttta tctaatggtg ctactgcaga
     3241 agttgaaaca gttactcaac aaagtggcgt tacaccaaat aatttcacaa atccagttac
     3301 ctataccgta actgcacaaa atggtacaac tactaaagat tggatagtaa ctgtaactgt
     3361 agctggagaa ccacttagct ctgaagcaga tatcttaaca ttctctttcg ctgaacagtt
     3421 tgaagaagca attattaaca atgttaatag aacagttaat ataaaggtta atgaaggtac
     3481 aaacctcaca aatcttgttg ctacatttac tctatcagaa ggtgctattg caaaagttgc
     3541 tggacttgtt caacaaagtg gcgttacacc aaataatttt acaaatattg ttatttattc
     3601 tgtaactgca gaagatggca caactgttaa aaattgggca gttaatgtga gtgaacattc
     3661 tggaattgaa gacatagaaa tttctaagct ttcaatttat cctaatccta acaatggtag
     3721 atttacttta gactttagcg atatctatgg taaagtaaac tatcaaatat atgatacaaa
     3781 aggaagcatt attctaagtg ataacttagt tgtaaacgga aatgcaatga aagaagtttc
     3841 actaaatatt gctcctggag tttactacgt taaattaatt actgaaactc aaagcataat
     3901 tgaaaaattg gttgttgaat aagaaagtaa tttatgaata atttagaaaa aggctaactc
     3961 atttagagct agcctttttt tattactatt aaaaaatgct aatatcattc tattcaatat
     4021 cttagccgac aggcttatat ttgttttaaa aaaaaataac aaaatactcg tttatgtcaa
     4081 aaaaaaaatg taatattgct ttttaaaatg tgtgaagttt ttaacgcatg aaaaatagtg
     4141 aaattaaaat agtataaatt aaatttatta aattatgaga aaaattctaa caagattaac
     4201 actaatacta gtattttgta ttggtgttag tgcattttca aaactacaaa gccagattgt
     4261 tgtggctaag tgggattttg gaaatacaac gaaacaagat gctattaccg acaatgctag
     4321 ctttgcaagt agtccctaca cagctgatga tggtattgtt gaaaataaag atattgcaac
     4381 aatatcttta ggtggtagtg ttaattataa cgggtgggta actggcgcaa gtggtacccc
     4441 agacaaagca cctaatagta ctgcttggaa tcttactgat ggctattggc aaatcggtat
     4501 tgttactaca gggtataaag atttaaccct aagttctaaa cagcaaagtt caaatactgg
     4561 acctacggat ttccaaattc aatatagtac tgataatggc tcaagttggt tagatgttgg
     4621 gactgctata actgttgcta ataattttac ttcaggtgta gtcacagatc ttgctcttcc
     4681 tgctgaatgt aataacttag ctaatttatt aattcgctgg acaaatacat ctaatgtgtc
     4741 tgtaggtggt actactgttg catctggagg tactaataga attgatgata ttgttgtaat
     4801 gggtacgctt gatgacccat gcttggcaac agtgcctttc tttgagggat ttgagaatgg
     4861 atataatcat gatgatgttg ttgaaggttg ttggacacaa gaaagtgaga caggaacagc
     4921 tacatgggtt gttaattcaa ctaatacaac ctataacaga actcctagaa ctggtttgtt
     4981 taatgctact ttacagtatt ctaataaaga ttggcttttt tataaagttt acttagaaca
     5041 aggcgagtct tatgatttta ctgtttgggc tagacaagat gcaacttctg gagctacgct
     5101 tactttagct tggggaacag aagataactc tgcagcaatg acaaatatta ttgttgatgc
     5161 tcaagtaatt actaatggtg attatcaaga agttaaaggg acttttggtg ttccaacaga
     5221 tggagtttat tatattggaa taaaaggaag tctaacatat actccttact atatgtcaat
     5281 tgatgatatt tcacttgatt ttggacctaa ttgtgttgag cctacagcta ttgtagcaag
     5341 tgagattaca gctaattctg caaatatctc ttggacagct tcagtatcag cccctgcttc
     5401 ttatcatgtg tattactcaa caactaatac agcaccagca gatgatatag ctgatgctga
     5461 atatgcagaa gtaactggtc ttacaactga attaagcgaa ttaacaccag taactacata
     5521 ttatgtttgg gtaagatctg cttgtggagc tgatgagaat agcgtttgga ctggtattag
     5581 cttcgaaact actcctagct gttttgaacc tacaaatatt caagcaagtg atattactat
     5641 aaattctgca aatatttctt ggacggcagc aactgaaagc ccagatgcta cttatcatgt
     5701 gtattactct ttggttgatg cagcaccagc tgacgatata gatatagcag aatgtgaagc
     5761 accagctact aactcaattg aattaactga tttaacatca agttcaacat attatgtttg
     5821 ggtaagatct gtttgtggag ctgacgattt tagtacttgg gctggaccat ctgttttctc
     5881 aacttcttgt gcaattatta gtgaatatcc ttatttcgaa ggatttaact ctggtataga
     5941 tccagcttgt tggactatta tagataatga taatagtggt gattcatggt attcaggcac
     6001 ttatgcatca acaccgtctt atgaaggtgc gtctatgatg gtttctgaat ttagtgagcc
     6061 aaatgatgat tggttgattt ctccacaatt taaaattact agcgataatt taaaagtaga
     6121 tttctatgca aaaagctata gtgcttattt ttatgaatct tttaatgttc ttatatcaaa
     6181 aacaggtaca tctccatctg attttacaat agttctagaa aatattgttg atcaccctaa
     6241 tacttggcaa aatcatgagt atatattatc agaacatggc attaatatag atgatgagat
     6301 ttatgttgca attcaacatg tttctaacga tcaatttgtt ttattcatag atgcatttac
     6361 agtatcagaa atagaaatac ctagtgcaga agcagaaatc ttaacttatt ctattccaaa
     6421 tcaagttgga gatactgaaa ttactagcac agaaacagaa ggaacaatta ctgtaactat
     6481 gccttatgat acagaagtag ctaatcttgt agctaacttt actttatctt atggtgctac
     6541 tgcaaaagtt ggtgcaatag atcaagaaac tggtgttact ccaaacaact ttacagatcc
     6601 tgttgtttat gttgtaacag cagaagacgg tacaacaact aaaaactgga ctgttactgt
     6661 taatctagaa gcagcaccaa gttcagaagc aaacatctta acttactctt ttgaagaaca
     6721 atattcacca gcagttattg atgatgttaa tcatacagtt actgttgtgg ttaatgaagg
     6781 tacaagttta gatgctcttg ttgctacatt tactttatct ccaggtgcta gtgcaaaaat
     6841 tggagcagtt gatcaagaaa gtggtgttac agaaaacaat ttcactgatc cagttatata
     6901 tactgtaact gctgaagatg gtacaactac taaagattgg acagttactg ttagtgttta
     6961 tgtaggaatt gaagatgtag aaaattctgc tctagcaatc tatccaaatc ctaacaatgg
     7021 tagatttact ttagacttta gcgatatcta tggtaaagta aactatcaaa tatatgatac
     7081 aaaaggaagc attattctaa gtgataactt agttgtaaac ggaaatgcaa tgaaagaagt
     7141 ttcactaaat attgctcctg gagtttacta cgttaaatta attactgaaa ctcaaagcct
     7201 aattgaaaaa ttggttgttg agtaaaacag taatttctaa ataattaaaa aaaaccgctt
     7261 caataacgaa gcggtttttt tttgctcttt tataagtttt tcttggtatt tttattattt
     7321 tgcattactt tcgatttttt aaagtaaaat gatatgacaa tacaagaatt aaataaagct
     7381 tttgaactag cttacggtaa acctgccgaa gctatttatt ttgctccggg tcgtgtaaac
     7441 ttaattggtg agcatacaga ttataatggg ggaccagttt ttccatgtgc attaagtttt
     7501 ggtactaaac ttttaattgc taaaaacaat aaaaaagtga tgaatttcaa atcgcttaat
     7561 ctgcctgaag ttgaatcatt aaagtttgac caactgacaa cccgtctcga aaattcatgg
     7621 gtaaattatc ctttgggagt aatggcgtag tttattaaga gaggaaaaac ttttacacat
     7681 ggttatgata ttttgatttg gggaaatgtc cctaacggag cgggattatc atcatcagca
     7741 gctttagaag tggttactgc atttgcttta aatgaccaat taaaagcagg tttcagtcga
     7801 gaagaaattg ctttgattgg acaaaaagcc gagcatgaat ttgctttggt aaattgtggc
     7861 atcatggatc aatttgtgtc ggctcatggt gctaaagatc atgcggtata tttggatact
     7921 acaactttgg aatatgaatt agttccagta atattagaag gtgttaaaat tgttatttca
     7981 aatactcact ctcctcataa attagattca ggtgcttata atcaacgtgt tgcagaatgt
     8041 aaaaaagccg ttgagcaact aaataatgtt cgcccaatta aatttttggc agagctaaca
     8101 gaagctgatt ttaaaaaaat tgaatctgct attaccgatc ctgttgcaca caagcgtgct
     8161 cgtcatgttg ttggtgaagt gcaacgtact gccgatgcag taaaagcatt gaaagcaggt
     8221 gatttaaaac ttttcggcga acttatgaat gcatctcatg tttctttacg tgatgattat
     8281 gaagtaacag gtccagaact agactgcatg gcagaagaag catggaaaat agacggagtg
     8341 attggttcac gcatgacagg cggaggtttc ggtggctgta ctgtttcttt agtaaaagac
     8401 gaagctatcg atacttttat aaaagaggta ggagctaatt acgaaaaaag aataggcata
     8461 aaaccagact tctatatcgc cgaaattgga gatggtgcta ctagattagt ttgataattt
     8521 gtaaaattaa aacatgaaaa atattaacac ttacactatc aaaaataaaa atggcatgac
     8581 tgccgagcta acaaactatg gtacaaaaat tacccgcttg ttagtaattg ataaatacgg
     8641 aaaattcgat gatattgtac aaggcttcga tactttgcaa gaaaacattg ataaagaacc
     8701 ttattttgga gcaacttgtg gacgtttcgc caatcgtatc aaaaacggtc aatttacctt
     8761 ggacagcgta aaatatactt tagctcaaaa taatggaggc aatgctttac acggaggaat
     8821 tgtaggcttt aatgccaaag tttgggatgt aatcaaacag accgacaaca gtattagtat
     8881 gcagtatatc tctcctgatg gcgaagaagg ttatccagga acattaataa cagtagtaaa
     8941 atttaccatt tccgaagcaa atgagctaca gattgaatac aaagcaacaa ccgataaagt
     9001 aactataata ggcttgacca accactcata tttcaattta aaaggagccg gcaatggaac
     9061 agtacgcaat catattttac aagtcaatgc tgattttcac acagtgttag acgatacggc
     9121 ttgccctaca ggtgaaattc gaccagtaga taattctcct ttcgatttcc gaaaaccaac
     9181 ggttatagaa aaatacattg atgccgagga ataccttcca gggcgtggta tagataataa
     9241 ttgggcaata cgtaaaaatc aaccaaaaga attggctttg gcagctactt tatacgaacc
     9301 aacaagcggt cgtgttatgg aggtgctgac tacacaacca ggtgtacaag tttacaccgc
     9361 aaattgggta gaaaaacaaa tcggcaaata tggaaagact tatgcaaagc aaaactccat
     9421 ctgcattgaa acacaaaact ttcctaattc tccaaacata gctcatttcc ctagctcgat
     9481 tttacgaccg ggagaaatat atgaagaaat gtgtatttat aaattctcag taaaatagaa
     9541 aatttaaaat attgttaagc ttgataagaa attacttttt tatgtgtaat aagtaataat
     9601 tctaaaatta tatttatatt tgtaacatat tttttaaagc aaattactat gaataaaaat
     9661 acaaataaag aaccaagagt aaaaggtttg ggttgtttag gtttaacagt ttttgttgtg
     9721 cctataattc taattttagt cgcattttta atttgggcag gctattatgc ctattcttct
     9781 tacaacaaat ttattgatgc tgatcaaatg gtgaaaaact cttgggcaaa tgttgaaaat
     9841 acatatcaaa aacgttatga cttaattcct aatttagtcg aaactgttaa aggatatgcc
     9901 acacatgaga gagaaacttt tacagcagtt acagaagcta gagcaaaagc ttcatcaatg
     9961 aatattagtg cagaagagtt gacagaagaa aatttgcgag ctttcgagga cgctcaatca
    10021 gaacttagcg gagccttatc cagactcttg gttagtgttg aagcatatcc agaattgcaa
    10081 gcaaaccaaa actttatgca attacaatct gatttgaaag aaattgaaaa tgaaattttg
    10141 caaagaagag atgagtataa taacactgta aaaccatata ataatttggt cttgaaattc
    10201 ccacgaaact tatttgcaaa aatgttcgga tttaaagaaa aaccatactt taaagcaaac
    10261 gaaaaagcaa gcgaaagccc aaaagtttct ttttaaaatt ttagataata atctttagtt
    10321 aattctttat attcttcaga atagtcgtgt ctgaagtcat tttctattgt taagtgcgaa
    10381 ttttcgcaaa tagaaaactg atggctaagt tcaaaaatta ttctattagc ttgtttacct
    10441 tttttaggaa tgataaaaag tttttttgaa acaaataatt ctgcattttc acattcttga
    10501 ataaaactat cgtgttgcaa agccggaaaa attaaataag ctaatccgtt tttatttaac
    10561 agtcgcttga tatttagcag taaatctgac agttttaatt cgttgtcgtg tttggcaata
    10621 gccttgcctt tatttggtga agcatttcca gccgaaaaat aaggaggatt tgaaacaatt
    10681 atatcatatt taatatctgt ttcgaaattt tgaacagctt ggcaaaaaat tgtaagtcta
    10741 ttagaccaat tagaattttg aaagttttcg tttgctaatt tacaagcttc agaattaata
    10801 tctaaagcat gaataatagc ttcagatttt tgagctagag ccaaagcaat aatacctgtt
    10861 cctgtgccaa tatctaagat gtgttttgca tctttacaat caatataagc acctagcaaa
    10921 acagcgtcag tgccaacttt tagcagagaa gaactttgtt ttaaactaaa ttttttaaaa
    10981 tgaaaaatat ctttcacaac ttaaaaatct tcgtttacac caataccaaa aagggcaaaa
    11041 tcatatttta tcggatcttt atggtcgaac tctctaagtt tttgcgttaa taaatcaaca
    11101 gctcgccagt cgttttgatt tctttctaac aaacccaaca atcttgcaat tcttcctgaa
    11161 tgaacatcta aaggaatgta aagacaagca ggagaaatgt ttttccaaat gccaaagtca
    11221 acacctcttt tgtcgtttct aaccatccaa cgtaaaaaca tatttagtcg ttttgctgca
    11281 gaaccagaag caacatttgc taaatgaggt tttgttcttt gtaaatgtgg aatagaaaaa
    11341 aatatctcat ggaaatcttt taaattatca aaaatattat ctgtttttgt taaaacattt
    11401 tctaatccac catgattttc ataaatgttt tttaatgatg taataaaaaa tttaaaatct
    11461 tcattattaa aagttctatg aatattagta ggaaaattgt caagatcgtt tttagaatga
    11521 gacattacaa attcataagg gttattgccc attaagttca tcattttttt cgaagatgct
    11581 ataatagtgc ttcgctttcc ccatgcaatt gtggcagcaa gaaaaccact tatttcaata
    11641 tcttcttttt tattaaatga atgtggaatt tgtataggat cattctcaat aaaattggga
    11701 ttgttgaatt tatcgtgttt ctcgtctaaa aattctttaa gatttgaaaa atctaatttt
    11761 gtattcatac taaaaaatta ataattcgaa tataatattt attttcaaaa tattgaaaga
    11821 tttattttct ttgtactatg attttaacaa atttatggaa aggtataatt gttggcttgt
    11881 cggcatcaat tccacttggt ccgataggag taatctgcat tcaaagaaca ttaaataaag
    11941 gcagacgttc tggctttata agtggtttag gagcagccgt agcagatgga ctttatgctt
    12001 ttattgctgg atttggtgtt aaaatcgtaa tggattcaat aaatgaatat caaattttta
    12061 ttagaagtat tggaggagta tttcttcttt ttatgagcta taaactttat gtaacaaatc
    12121 caggagtaca actaagaaaa caattgaaga aaaaacgtag cggtttgttg ggagatttct
    12181 tttcagtatt tgctttaaca atttctaatc caatcacttt atttgtcttt gctgtagtgt
    12241 ttgcaggatt taatgtggtt gataaaactt cagacctgat agtagttata ctattactta
    12301 taggtgtatt attaggtgcg gtattatggt ggtttacttt aacctcaata gtaaataaat
    12361 ttcgaggaaa attcaaactc agaagaatgt tgtatataaa tagagtggca gcaatactta
    12421 tagctctatt cgcaatatct ataatagttt ctatattttt tgataaaagt atataagact
    12481 aaaataaaga ggctaactca agctaaaatt taatcttttt tcaaaacttc aagcattttc
    12541 ttaatagtat ttttagagga ataattatat ccgtattttt caccatttat aatatgtccc
    12601 aatccatttg gatagcgact ataaacaaca gattcttctt caaactcaga agccgaaaaa
    12661 tgaaattcat aaattccaac atctttcagg atttttgcat tttcaaaatt taaaccgcca
    12721 cctggcatta tgcaaatttt gttgccatat tttttatgca ttataataat attttccaat
    12781 ccttcattaa ttttagcgca accaccagaa gttaaaacag cttttgcacc tatctcgcat
    12841 aaaaaatcca tagcttcatc aatatcggta caaaaatcta ttcctctatg aaaagtaaaa
    12901 ctttgaggct tactaagttc aagcaatttt ttagtccttt ctttgtcaat tcttccattt
    12961 tctaataaaa ccccagaaac aattccagct actccggctc gtttcatctc tttaagttct
    13021 tcgcacataa tttcaaattc tatatcagaa taaacaaaat tacctgctcg aggacgtaaa
    13081 agtgcattta ttggaatgtc tgaaatttca atagcttgtt tcaaaaaaga atagcttgga
    13141 cttatacctc cagtgtttaa agcggtacac aattctattc tttgagcttt ggcattatta
    13201 gcatttttta ccgatttgat agtgtcgcaa caaatttcta aaattttttt cattatattt
    13261 gtaaataaaa tatttttggc acgttttttc ttgttacaaa ggtaaatata atattatgag
    13321 aacaatcttt tatagcatat ttttaatttt gataagcaca aatattatgg ctcaggaaga
    13381 aagattttcg tttccagacc aattaaagtg cagattcatg cctaaacatg ctaaagatac
    13441 agctgtgttt ttcgcgaata aaggaatttg gcatggatat actttgccag aaaataatga
    13501 tacaaaaaat tgtggaaaat ttggcggacc atattgtttt tatacaaaaa aatggtttgc
    13561 tccaagtctt ttagatttaa gtttaaatgt tgcaggacaa ggaaaaatag atttgaatac
    13621 tgcaaaaaca aaaaatttaa accaatttcc aggtttgctt atgcaagaat ttgaatttga
    13681 tgattataaa ttaattttac aattagaagc agtaactacg cgtgcaagtc tgtatcaagc
    13741 tattcttata aatacttcag atgaagcaaa aaagttttct atgcaactcg aaggtaatat
    13801 atttgataat acaagcaaaa ttgaacaaat tccagatggc tggatatata aagttgatgg
    13861 gcaagaagat ataatttggt ttgtgagatt taggttagac gctcaaatgg agttaaatta
    13921 ttctgacact aattacgaat ttaaatataa ggcaatgcaa acagtgcaag caaatgatag
    13981 cttgaaaata acagctgttg tcgctcaatt ttttaatgga gatttacaac aagacgtatt
    14041 gattacaaat gatatacttt ctacgccaca actctatgtt gataacaata aaaatttctg
    14101 gaatttctta tttgggcata tgactacaca aagtaatatc aggcggcaga tgactttaag
    14161 tgcttttcaa acaatttatt tgaactcgag atctgcattg ccaacaaaaa aatatttaac
    14221 agttttgcct tatgcggaaa cgaaagactt gtttataaat acagatgaag catggtttac
    14281 cgcagtgtct ttattaaaat tcgaccctcg tcttagttat aatattgtca gttttatttt
    14341 aaattcaata aataaagatg gagctttgaa aaaatattta agcttagtgc caaaaattga
    14401 tgataaaggt gaaattattg ataaacctat ggcagcatgg acagttttta atatttattc
    14461 ggtaactcaa gatgatgaat ttgttaaaat gtgtttgcca tttttagata aatatttaag
    14521 ctattggttc aaagatagag atgttgataa aaatggctgg gtagaaaatt ctcagggaat
    14581 agaagaggtt gcattaaatg caattttatt taccgaaact cactctatgt taaaaatgta
    14641 tgaagctata gatgatgaag aaaaaataga atattatcaa aaacaagtcg ctagaatagc
    14701 agataatttt aatctatatt ttttccaagt tgatcacaat agatactgtg attttaatgc
    14761 agaaactcaa gaatatactg ttgcagaaaa cacaattgga tattgtttat ggtcgggaat
    14821 tgcaaatcaa agtatagccg atgtttatgc acaagaaata gcaaaagata taaattcaga
    14881 tgagattttt tatagactag caaattctga catagatcca ggatatttat acttaatggc
    14941 atttggcttt aaattattta attatgcaca aattcaggaa gctctaaaat caatttatac
    15001 caacagtgtt gactttgaat ttatgaataa taataaattg tttgtcaaaa aatacgattc
    15061 caaaactgta gaatactcaa catatatggc tgctgtttat ttattgatgg caaactattt
    15121 ctaaaaaata atttttaaaa ttatcaattc ttaattcttc aattcttaat tcttaattaa
    15181 ataatcatgt tgtaaaatag ttaacatttt acttaaaatt caaataagtt tattagtttt
    15241 gctaaaaata ttaaattatt attgacaaat ttaaaaaaat aaaaaaaatt atgagaaaac
    15301 acatttttaa cgcaggtcct tgtaaattac cagacagaac tttagaaaat actgctaaag
    15361 caattttaga gtttaacaat tctggacaat caattatgga agtttcgcat cgcggaaaag
    15421 attttgttgc agtaatggac aatactattg agttaatgcg tgaaatttta aacattccag
    15481 aaaactattc tgtattgttt ttaggtggtg gtgcaagttt acaatttgca atgatacctt
    15541 acaactttat gaaatctaaa gcattttatt tagatactgg aacatggtca actgccgcaa
    15601 ttaaagaagc taaattgttt ggcgaagtta ttgacttatc ttcaaaagat aaagctttta
    15661 catatattcc taaaaatttc aaagttccta gcgatgttga ttatgtacat attacaacaa
    15721 ataacacaat tcgcggaaca gaaattcatg aagatttaac ttttgataaa cctcttttcg
    15781 ctgatatgtc gtcagatatt ttaagccgtc cagttgatgt tagcaaatat gctatgattt
    15841 atggtggagc acaaaaaaat atgggaccag caggagttac ttttgttatc cttagaaacg
    15901 atatgctaga caaagttgtt gaccgcccaa taccaacaat gttaaaatat tcaacacata
    15961 tagataaagg ttcaatgcac aatacaccac cagttgtaaa tatttacgca ctacgcgaaa
    16021 ctttactttg ggttaaagaa caaggtggag taaaagcaat ggataaattg tcaaatgaaa
    16081 aagctacaat gttatataat gctattgatg aaagtaaatt atttacttgt ccagtagtta
    16141 aagaagacag atctagaatg aatgttatct ttgttcttga agaaaaatac aaacacttag
    16201 aagaagattt tgtgaaattt gctacttcaa aaaattgtgt gggaattaaa ggacacagat
    16261 ctgtaggtgg ctttagagca tcgatttata acgcatgtac tgttgaagac gtaaaagctc
    16321 ttgtagaagt tattaaagaa tttgacaaac aacacgctta aaaataatga tgtcctcttt
    16381 agttaaaaag aggcagtcta aaacaattaa ggtgtcttag aagtgttcaa gtgtggatac
    16441 ttttgagaca ctttttttat tttataataa ctaaaaaaag taaaattact cccaaattat
    16501 tatatttgta aaaaaattca gacaaatgtt tcttaaaatt gttttattaa tagctgtgat
    16561 ttttcaaata attgcagtga tagtgtcgtt aagacttact agaaaaacaa aatataatct
    16621 ctcgtggata tttataagct taggatttgg cgctcttttg atgagaatga ttttagaagc
    16681 tcttcctttt tatttcaatc tagaaccaaa ggcgtatgga tacttataca tgtggctagg
    16741 agttgcgtca gcaatgttct ttgcaatagg actaatactt ataagaagta tttttacata
    16801 tatggaaaaa attgaaaaag aaaaacgtgc aagcgaaaaa cgttttttat ctgttttaat
    16861 tcaagccgaa gataatgaaa gaagaagatt agcaaaagat ttacacgatt gtataggacc
    16921 tttactatca acaataaaaa tgtctttgtc ggctttgaaa aaacttaata ccgatgaaca
    16981 acaaaaagtt attttagata atcttgatac agttgttgtc gaatcaatga aaagtgttaa
    17041 agaaatttca aataacttaa atcctcatgt acttgataat tttggcttag ataaagcaat
    17101 tgctaatttt atcccaaaaa ttacaacaac aggagatcta aagataaaat ataatagtag
    17161 tttacaagat ttacgtatag aaactagtaa ggaaactgtt ttatatagag tggtttgtga
    17221 actaataaac aatacaataa aacacgcaaa cgcaacccaa atagatatag atttgtatta
    17281 tgatacacgt aagatttatt taacttacaa agataatggc agtggcttcg aattggataa
    17341 tttattcaaa catcaagata aaggaacagg aatttacaat atttataata gaattaattc
    17401 tttaaaagga actgtaaata ttgactcaaa actggattgt ggcactaaag tagaaattaa
    17461 aataccatac tcaaatgaaa acaattaaaa aaacatatag cctaatgatt gttgacgacc
    17521 atgccttatt taggaatgga ctaaaactaa tattaagctc tctgcctaac tttaaagtag
    17581 ttgccgaagc ttcaaatgga gaggaatttc ttaatacttt agatttaatt ccagtcgata
    17641 ttgttttaat ggacatagaa atgcctgtat taaatggaat tgaagcaaca aaactagctc
    17701 taaaaaaaca gccaaatctt aaaataatta cgctttcaat gtatggagac gaagaatatt
    17761 atcacaaaat gatagatgca ggagcaagcg gctttttgtt aaaagattct gatattgacg
    17821 aagtacattc agcattaaat tctgtagcag aagggaaaag ctattttagc caagaactat
    17881 tgcaaaattt aattaaaaat tttcaaaatc aagacgaaaa tcctaaagaa acatttgatt
    17941 tgtctgcaag agaagtggaa attatagact tgatttgtca aggattttca aatatagaaa
    18001 taggcgaaaa attattttta agtaaacgaa cagtagaaaa acatagagct aatattttag
    18061 aaaaaacaaa tacaaaaaat acagcaagtt tggtaatgtt tgcaataaaa aataaaattg
    18121 tagatgttga gcaataaaat cgaaaatata ttggtttata atacataatc tattacataa
    18181 ttgttatgag gtatttaaaa attttgttta ttttattttt attgcaatcc aatttgtata
    18241 gtcaagtagt taaatataag tcggatttgc cagtagtata ttatagagat tcgctgcaaa
    18301 aagcaggtga atataaattg tcattgcaaa atcatttgtc ttctttgagt aaagagttta
    18361 cttgcgaagc attgtataaa attgctgtaa attatgctga acttaattta aaagattctg
    18421 cattgtatta tttgaatctt tatgttgacc actctagcga tgatagactt attcttgctg
    18481 ataaagcttt tgagccttta aaggacaaaa aagcagattg gcacaaatta gaaataaaaa
    18541 taaaaaactc ttatttaaaa tctttgcctg ataatataaa tgaaaacttg gctttagaat
    18601 tgtttgagct tagtattttg caaaatttat atgaatatta tattgattta ttaaaaatgc
    18661 caaattctgc ttttgaaact aaaaatgaaa tggaatcctc tatgttttct aatccacgtt
    18721 taaaaaagaa acttatacag aacaagtatt gcctaacata atgaaaagtg aaaatatttt
    18781 taaagctatt ttacaaaaat atggtttccc aagcgttgaa aaagccggag tgtttgggga
    18841 aaacattgcg tataaatcaa taatgaaatt aaatgtttct gagcaatatc ttgattctgt
    18901 tgaaaattta tttaataaaa aattatttga tcctcttgct tatgcgatga taaaagataa
    18961 gtatctctat atgaaagata aaaaacagga atttggaaca ctcctttatt actgtgaaaa
    19021 tgatagtaaa tgggaactta gcctttatcc tgtttcagat tttaaaaatt taaaccaaag
    19081 acgaaaagaa attggtattt tagaaacagt agaagattat gcaaatagac gagatgcaaa
    19141 aattcctaaa tcttattata aataagttaa agtaaatttt aaaatttgat atttgtttgt
    19201 tttctttgtg aaagattaaa ttattttaaa tataaaaaca ttattatgcg taaaattagt
    19261 tttttagttt ttggcttagc ttgtataata ttattgtctt cttgtggagg accaaaaact
    19321 gatgcaaaaa agttagaaac attattaaaa gctcatactc aagcatttgt tgaaatagca
    19381 agcgataata aaattgatga aaaagaagca aaagaagttt ctaaactaat ggaagagatg
    19441 agaaatttta attcagaaat tgaaaaaaaa tatgaatccg atccaaaagg taaagaaatg
    19501 cttgaagaat atttcaataa aaacgaagaa aatttcagtt taatttatac cgattattat
    19561 aattctcttt tcggtttatt taattgtgaa ggttctgaaa atttagattt gtaaaaattt
    19621 tattcagatt cttcagtaaa ttttatatcg aaagcaccat ctgaaattgc aatagtttcg
    19681 tcattttcat ttttgtaatt aaagaaaaac gttcctctaa atttttttgt tgttgaatta
    19741 ttttcaacaa tagttacaga gccattagta gcatcgtaag tagaaaatcc tttttgaaat
    19801 ttcgcataaa ttggaccatc ggcagttata taatgtacac cagagccaat agttggcgac
    19861 atccacattg taacgctttg ccacgtttcg gttctgtcgc aatcagcaaa gatgactaaa
    19921 gctgtgtcat ttttttgagc agtaatatct tttgcatacc aagttctacc ttcagtgctg
    19981 taataaaaaa tgttttgata agtggtatca gaattactgt aaaaatgata aggaacatta
    20041 gtaaaaacgc cgtcattaat tgttctaaaa gtattgtcag tgcttctata tgctttaaaa
    20101 ctaaaactac cactcatagt tttagattct tcgtttattg attcaatttt gatagttcca
    20161 taagcacttt cactgtttaa agtagaatat cttgcagcgc cagcactcat atttgggata
    20221 aattcggcat aacaaccagt aaaagcattt gcggtatatt cgcctttttg tggagaacca
    20281 acatttaaaa taatagtttg tccattagct gaagtaccaa atacttttat agaagtttct
    20341 gttaaaattg cttttggttc tggtgaacgc cagtttgtcg aatttacaag acaaaacatt
    20401 gctggttttg cagaattatc gcctccacta tttccacaag aacagaaaaa ggcaaaaaca
    20461 aaaataataa ctgataaaaa aataatttgt ttcataaagt aagtttttta caaatataaa
    20521 taaaataaat taacccgata tgctcgtaaa ttaaaatcaa ttaaacttaa agtgttaata
    20581 ataatttttt tatttttttc tcctatacaa gatgtttatt ccgccaatca ctatcggaat
    20641 tagaatgtta acaatataaa tcaacaagct agctatgcta attgcagaaa ttttattggt
    20701 aaaaagtcca aaaaataaaa ttgaaaacga aattttaatt cctacttcca caattgcaat
    20761 attgggtaat agatgtgaag caaaaaacat tgccgaactt gcaataaaac aattataaaa
    20821 actaatatca attccaaaaa ttttgagagc cacaaaatat tgaaagctaa aaactaaata
    20881 tcttaacaag cttaaagaaa ataaaattaa tttgtctttg aaacttatta ttggcaagtc
    20941 gtaaaatttc gaaaaccaat ttggcttgaa atttctattt ttaaaaaagt tgataatttt
    21001 attaattgaa aaaaacaccc caaaacttag agtaaaactc aatataataa acgtatagac
    21061 aatgcttttg ttaaaaatta aatttgaatc tttgtcaaaa taaggtatca gtaataaaat
    21121 cgctaaactt ccaaaaaata ttgtaattag tagttgcgac aaatcaccaa ttaaagttaa
    21181 cgaactagct ctttttctat tttctggttt tagaaacatt actctaccaa caaattcacc
    21241 aattctattt ggagtaagtg tgccaatgct aacacctgtc caaacaaagg ataaagctgt
    21301 taatggtttt agcttttcaa ttttagaaat aataaatctc catttttctg actctaaaag
    21361 ccagttagcg agcattaaaa ttattagtaa aatcaaatat gaaatttgtg tggaattaaa
    21421 ttcgaaaaga agttttaata gttctggaaa atcactactg ttttttagtt tgaaataggc
    21481 aaaaagcaaa attaaagcaa caataattaa ttttactaaa aagagtatgt gattttttaa
    21541 acttgatttt ttcaaggatt attttttatt taaaatcggt agtaaaataa atgataaaac
    21601 tcctagcagt attaataaaa cagtttgaat tccatgcgta atcaatgcaa aactagctgc
    21661 acttgattct gctatgccat aaattattaa agtttgaatt accataaaat gataagcacc
    21721 aattccgtta ggagcaggag ctaacatacc caaactacca gcaacaaata taacaagagc
    21781 aacgctaagg tcaagatgtt gcatgctatc aaaggcaaaa aagcaagaat aaagcataag
    21841 aaaatataat atccaaataa aaaacgaatg aaaaataaac ttagctttat tgttgatatt
    21901 ttttatgcta attaatcctt cccaaagttg atttaagaaa tttgaaattt ttgcaaaaat
    21961 tttatactta ttatattttc cttttgcaat tttaactatg aaaaaaatgc ctatggctat
    22021 tattattgta cttaaaacaa taaatttcca tgaaaacaaa aattccaaat tgtcgccaaa
    22081 ctttggattt tttcttaaaa attctaaaac ttggtcatat ccaacaataa ctccaataac
    22141 cgcgaaaata aagaatatta ttaagtcaat agctctttcg ctaatcatgg tgcctagaac
    22201 tttagcaaaa ggaatttttt catattttga aattatgcca cagcgagtaa cttctcctaa
    22261 acgaggcaat gcaaggtttg caaaataacc attagcaacg gctagaaaag tattagaaaa
    22321 tcttgtttta tttccttcgg tttctaaaag cattttccaa cgaaaagttc taaaaaaatg
    22381 actaaacaaa ccaagaacag ccattaataa aaaccatttg taatctaatg ttttagcttc
    22441 ttcaactaat tcaacaaaat caaaatcttt atacaccaat aaaaatagca cagcacctat
    22501 gccaatgaaa atgatgaatt tgatgatatt ttttatcgta gttttcaaaa gtttttttgc
    22561 aaaaatataa aatatattta aaaacccaag tccaagttgt tttttgaaat tataactttg
    22621 taaactttac aaactttacg aactttacga actttacgaa ctttacgaac tttataaact
    22681 tttatatata tttgtattat gataaaagca aaaaaacatt tgggtcagca ttttttggca
    22741 gataaaaaca tagccattaa aattgttgat tctttacaga atttgagagg attgccagtg
    22801 ctcgaagttg gtcctggtac aggtgtgctt acaactgaat tagtgaaaaa agattttgat
    22861 cttaatttgg ttgagataga cactgatatg gtttgtcttt taaaggaaaa atttggcaaa
    22921 gaattaaatt taataaatgc agatttttta aaaatagatt tgaaaaaaat atttagttct
    22981 gagttctgca taattggaaa ttttccttac aatatttctt cgcaaatttt ctttaaaact
    23041 ttagataatg ttgagcttat accagaactt gtttgcatga ttcaaaaaga agtggcagaa
    23101 aggattgctt caaagcatgg aaataaaact tacggtattt tgtctgtgct tttacagttg
    23161 ttttataaaa ttgaatattt gtttgttgta aacgaaaatg tttttattcc acctccaaaa
    23221 gtaaaatcta gtgtaattcg tttaactcgt tatcgcacag aaattgaagg cgtggataaa
    23281 aagcaacttt ttttagttgt aaaaacagca tttaatcaaa ggcgaaaaac tttgagaaat
    23341 tctttaaaac cattgcttaa cgaaaaagtt gatactacaa acaaagtttt taatttacgt
    23401 ccagaacaac ttagtcccga agattttgtt tcgcttacaa aaatgatttt taaagataaa
    23461 aaaatgtttg tcagcgattt tgacggaact atagccaata atagctcggt agtaagcgaa
    23521 aatacaataa atttattgaa aaaacttgag gaaatttctg tgcttcgtgt tattgctacg
    23581 ggaagaagtt tgttttcgtt tacaactgtt gtaaaacaag actttccaat tgattattta
    23641 gttttttctt caggaattgg aatttatgat tggaaaacaa aaaagattat tcaatctagt
    23701 tgcatagggt cagaacaaac aaagaaagtt tatgactatt tggtggagaa caattacgac
    23761 tttatggttc agttgccagt accagataat catttttttc atcattttag ttctgattgt
    23821 ccaagttctg attttttgac tagagtaaag ttttatgaaa caaaagggct agagccaatt
    23881 tcagaatgtc ctgaaacagc ttcgcaattt gttgtgattt gttctgaaga aaatggacaa
    23941 atggaaaaaa tttctaaaaa attcccagat ttgaaagtcg taaaagcaac aagtccattg
    24001 gataataaat caatttggat agaaatttta ccatatcaaa tttctaaagc ttcaggaatt
    24061 gaatttattc gacaacttga aaacatagaa catgaaaata ttgtaactgt aggaaatgat
    24121 tactacgacc ttgatatgtt aaattactcc ttaaaagaaa actcttatct tgttgaaaac
    24181 tctccagacg aaataaaaga taactttaac attatagcat ctaacgaaaa tgagggagtg
    24241 gcaaaacttt tacaacgaat ttattcagtt aattaagtgg taattttagc tttcaaatcc
    24301 gaacgagctg gtgcggtttg tccgtaggca acacaagact gtcaaaggct tgttcgtatg
    24361 acaacatgtg attaaccgta ggcgacagcc tgcggtagat gtcccctcga aagccttgcc
    24421 cgcaagggca acacaataaa atcagttaaa agtttataaa gtaaaaagtt tgtaaagaaa
    24481 tgctaaacag aaaaccgctt ttaactttag acttttaact tttaaatgta ttgtaagatg
    24541 gtacaataaa attgaaattg tataggtaag agacggcgca tgcaccgtct ctacactata
    24601 ccatataatt ctttcaaatt tatattttta cagaaataat tttcaattat ttgaaaatag
    24661 ttgtattaaa tttctcaaca gcaacacttt tttgttcccc agttttcata tttttaattg
    24721 aaacagtttc attcttaatc tcgtttgctc caattattac aacaaaattt atatttttgt
    24781 tattagcata tttaaactgt ttatcaagtt ttgcattatc agggtaaatt tcgcaactaa
    24841 cgccgtcttc tcttaatttt ttggctaatt ttatagcaag tttttcttct tcgcttccaa
    24901 aatttacaaa taaaacctct acaggagaat taattgtttc agggaattta tctaagctgg
    24961 ctaaaacatc ataaattcta tctgcaccaa aagaaatgcc aacacccgaa acatctttta
    25021 aaccaaaaat tcctgttaaa ttatcatatc taccgccacc taaaatactt ccaatttcta
    25081 catctttggc tttaacttca aaaattgccc cagtataata atttaaacct cttgctaagc
    25141 taatatctaa ctcaacttct gtatctaatg gtgaaatttt aagataatca aatatggatt
    25201 ttatttcttc tatgcctttt aagccagctt cgctattttg tagcaacgac tctagaaatt
    25261 ctatggtttc agaactattg cgtttgtttt ctaaaatagg aataagtttg tttatggctt
    25321 catcagataa accattgtct ttcagttctt tgcaaacgtt ttctaaacca attttatcaa
    25381 ttttatcaat agctatggta atttccataa atttatcggg agcaccaaca acttctgcaa
    25441 ttccttctaa aatttttcta ttatttattt ttaaaacaac agcaatatca agtttctcga
    25501 aaacttcatc aataatttgc ataagttcaa actcattaag cattgaatct gaaccaatta
    25561 catcgacatc acattgataa aattcgcgat aacgtccttt ttgaggtctg tcggcacgcc
    25621 aaacagcttg tatttggtat cttttaaatg gaaattgaat ttcgtttcta tgttgaacta
    25681 caaaacgagc aaaaggaact gtaaggtcgt atctcaaacc tttatctgaa atttttgagc
    25741 ttaatttatt aaggttttct tcgcctaaat catcagcatt tacatttttt gaaaaatttc
    25801 cagaatttaa aattttaaac aataatttat cgccttcgtc gccgtatttt cctaacaaag
    25861 tgctaaggtt ttccatagct ggagtttcga taggcgagta gccatatttt ttaaaaactg
    25921 actttattgt ttcaaaaata taattgcggc gcatcatttc taatgtgccg aaatctctag
    25981 tgccttttgg tattgatgtt ggttgtgcca taataattat acgttaaatc taatatgtaa
    26041 aatgtcgccg tcttcaacaa cgtaagtttt tccttctaca tgaaatttgc ctgcattttt
    26101 aactgctaat tctgaaccta aacttacaaa atcatcatat ttcataactt cggctctgat
    26161 aaatcctctt tgaaggtcag agtgaattac tccagcagct tcgggagcag tcattccttt
    26221 tgtaattgtc caagctctaa tttcttttgg tccaacggtg aaaaatgact gtaaatttaa
    26281 taaatcataa gcctcgcgaa taattttatt tacactaggt tcgcttagtc cgtaatcgtt
    26341 taaaaactct tgacgctctt catctgtttc taactctgca atttcagact ccaattttgc
    26401 agcaacagtt aaaatcttta cattttgatc ttttaaatat tcttttacgc tttcaacatg
    26461 cttattgcct tcagctatcg agtcttcatc aatattgcat acaaacatta caggtttttc
    26521 ggttagtaaa gataattctg ccacatgttt tctatcttct tcgcttacag gtgcagttct
    26581 agcgttttgc atgttttcca aatgttcttg atagattttc agaatttcaa tacttgcttt
    26641 agcgtcttta tcgccaactt ttataagttt ttctaatctt gcaatttttt ttgtaattga
    26701 ttctaaatct tttacttgaa gttctaaatc aatggtttct atatcacgaa cagggtcaac
    26761 cttgccttcg atatgaggta aattttcatc ttcaaaacat cttaaaacat gaataatagc
    26821 atcagcattt ctaaggtcgg caagaaattg atttccaaca ccttcgccag agctagaacc
    26881 ttttgttaaa ccaggaatgt caacaatatc aactgttgca ggaatgattt tttcagtttt
    26941 ttgaaatttc tcaagttctt ttaatctagt atcaggaaca tttataacac cgatattaga
    27001 cttattactt gcaaaagcaa agctagttgt ttcggctttt gtattcgaca tacaattaaa
    27061 taccgtagtc ttaccacaat tcgcaatacc tataattcca caatgtaaag ccattttaaa
    27121 aattctaaag tttaattttg caaaaataat caattttgcc aaaaactcac aaacttcaaa
    27181 ctagaactga aataaatttc aaacaaatat tttaattaat tttaacgtaa actaaataat
    27241 ttttttgtta tttcgttgtt atgatattta tatagaatat tgaaaacaaa aacaagtgta
    27301 attatcattg ctttgttggg cttgttttgc ttgaaggcaa atgctcaaat cataaaaatt
    27361 gctgataaaa tttttgtggc tggttatgta tttgaagaaa ataccaaaga gcctattcct
    27421 tacgtaaata tttatgtaaa gcaaacacga agaggaacaa ttacagacaa taaaggatat
    27481 tttatgctta aagcagaaat taacgacact ctaatttttt cttgtttggg ctttgataaa
    27541 aaatatgttc atataaatca ggaagttatt gacaataacg aggctttaat aatttcttta
    27601 gatacaaaaa tttacgaaat acaatctgtt gacatttatg ctttacgtaa atataagcaa
    27661 ttagagtatg aaattaaaag catgaaactt aaagatgatg atattgtaca tgcacaaagt
    27721 aattttcctt tacgacccgt tgatgttgat tattatgatt tagtacagtc tgcaccgctt
    27781 agtggatttg gtgttgttat tagccctata actgccttgt acgaagcatg ttctaaaaaa
    27841 ggaaaagaaa atcgtaaatt agcagaatta aaaaaacaag ataatattca aaatttgata
    27901 gagcaaaaac taggagtaga aaatattatg aaaattataa atgaagatga agcaactact
    27961 cttgatttta tagaatggtg cgatttccat gaagactttt tattgaatat ttctgcttac
    28021 gatttggcta cggttattaa gtataagtac aaagtttata aagttcatcc gagtttacga
    28081 gctcgctgaa agcaaagttc ataaagaaaa gccgaacacg tagcctcttt taactgaata
    28141 tttttgttga caacttttct ttaacaaaaa aatttgtgat tttaaataaa taaatctact
    28201 tttgcgtgta attaaaaact attttgggtt atgtatattt tgaaatctga aagtaccgac
    28261 ccctatttca acatcgcttt agaagaatac ttattaagta attataagga agattttttt
    28321 attgttgcag taaacgagcc ttcaataatt gttggtgtta atcaaaatac taacgataca
    28381 attaatgcac tttatgtaaa agtaaacaaa ctgaaagtcg ttagacgact gtcgggcgga
    28441 agtgctgttt ttcaagatga aggcaattta gatttttcaa atatttatac cgacagctct
    28501 gcaacattaa ttgattttac tagaataaca gaactgataa ttgaattttt aaaaacaaaa
    28561 tttaacatag aagccaatgc ctcgggaaga aacaatattt taattgacgg gaaaaaaata
    28621 agcggccagg caagagcaag atttggagaa aaaattttga atcatggaac gttacttttg
    28681 tcttcaaata aaaagtcttt ggtagatgct ttaaaattta atgaagaaaa atataaagac
    28741 caaaaattaa aatcaaattt agaaagaatt accaacatta atgaacatat agaaggaagt
    28801 attactatgg ataaatttat agatttattt ttaaatttta ttcaaagtaa atttccacaa
    28861 gctcaaagat ttaatcttag ccatgatgat atcaatgcag taaatgaatt agttaattta
    28921 aaatactcaa attgggattg gaattatggt aatatacctg aatacaatat caatacttcg
    28981 ttgaataccg tagatggatt tgttagtatg tacttgaatg ttgttgaaga caaaataaaa
    29041 aatataaaaa tttacggaga ttttttctca cagttaaaca tttccgattt ggaaaatgtt
    29101 ttagttggag tgaaatatga aaaatcagaa atagaaaagg cattagcaaa tttagatttt
    29161 aattgctata tgaaaggtat tgataaacaa gattttattt cacatttatt taaaaattaa
    29221 atgtaaagtt tcaccacgtt gttaatgaat ttatccccct gcttttgctc atgga
//
LOCUS       DAOPNX010000023        26629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_2333_length_26629_cov_5.784752, whole genome shotgun sequence.
ACCESSION   DAOPNX010000023 DAOPNX010000000
VERSION     DAOPNX010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 26629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26629
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2333_length_26629_cov_5.784752"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..603
                     /locus_tag="PKN53_04005"
     CDS             67..603
                     /locus_tag="PKN53_04005"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOD88148.1"
                     /translation="MIFLIYYPENKNRATIFSLILFNVVIFLLTFVLNDIKISMGAAF
                     GLFAVFSMLRYRTAGISLKDMTYLFIFIALGLVGAIGLKVQDLAIINGIIFIFIFVLD
                     GGFIFKREYSQIVVYEKIENIKVENYDILLEDLKERTGLNIHRIAIGKLNYLRDSAQI
                     KVFYYQKNKKVYPYGEEK"
     gene            complement(616..2013)
                     /locus_tag="PKN53_04010"
     CDS             complement(616..2013)
                     /locus_tag="PKN53_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOD88149.1"
                     /translation="MAKKDSIEKYKHLYKQSFWWNFFGLYVKTLHTLFYKNIVVEGKE
                     NIPKDCPLIFAPNHQNALMDPLAILFTFNRQIVFLARADIFKNKIFAKALYFLKILPV
                     FRIRDGKENLANNDLTFDIAVKVLESKQYIGIFPEARHNNKRQLLNFKKGIPRLAFMA
                     EEKNNFNLNLKIIPVGIYYSQYNRMRSIIHIRYGKPISVSDFKDEYFDNQQKAMLSLR
                     DKMQEATSNLTIDIQNLELYDTYESIRTLYVKNLIRKFQLGKLTQINKFKADKITINA
                     LNTFECDYPEKMLEIKSQVEHHESLKKKYKLSDQSIEKPFLSIFRLIINTLIIILCLP
                     IYIYGLLNNALVYFSPKLIVLKIKDKQFHSSIKFVWAVFLAPIIYLIQIVIFSLVIPN
                     IYLIIAYAISLPLTGLLAQLIFEWIIILVEDWRLLKLRYFNKSEYKKLKQTHKNLILK
                     LDSIINHWKKINQAN"
     gene            complement(1992..2936)
                     /locus_tag="PKN53_04015"
     CDS             complement(1992..2936)
                     /locus_tag="PKN53_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HOD88150.1"
                     /translation="MFEALQKMDGATLNFSDGGLLVLNIAIAVIMFGVALEIKVSHFK
                     ELFKKPKPVIVGFLSQFLVLPLLTFLLVMAFWENLTAGVAMGMILVAACPGGNVSNFL
                     STHAKGNAALSVSLTAIATMLAIVLTPFNFYFYGKLYKTFGPWVDNALLRELTINPIE
                     MLKTVIILLGIPIVLGMVVNHKFPKFTEKIKKPIKLFSLFFFMGMVVIMFANNYDHFV
                     KYIYWIFVVVLIHNALALSSGYGFAKLFRLNTINSRTIAIETGIQNSGLGLVLLFNPN
                     IFPQDLALGGMMIVTAWWGIWHIISGLTISTIWRRKTV"
     gene            3065..3553
                     /gene="cdd"
                     /locus_tag="PKN53_04020"
     CDS             3065..3553
                     /gene="cdd"
                     /locus_tag="PKN53_04020"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOD88151.1"
                     /translation="MKNHGINIRYKEFSYGELDSVDKKLLDSAVEAAKNAYAPYSHFR
                     VGAAVLLDDGSIITGNNQENAAYPSGLCAERVALFYANSKYPQKTVVAVAIVAVNPEG
                     EVLKGNVSPCGSCRQVMLETQTRYKNLIKIMLASDDAVLVFASVEDLLPLNFSQDSLK
                     GN"
     gene            complement(3632..5998)
                     /gene="pbpC"
                     /locus_tag="PKN53_04025"
     CDS             complement(3632..5998)
                     /gene="pbpC"
                     /locus_tag="PKN53_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HOD88152.1"
                     /translation="MKKAKILLIISITIWFAIILVFIFSLPSTLFKDPYSTAVYDKNN
                     QLLGVKIAEDGQWRFPETQKISPKFEACILNFEDQRFYYHFGIDPIAITRAIKQNISS
                     GKIKSGGSTISMQTIRIARKGKERTYYQKIIEVILAFRLELSYSKKEILQLYASHAPF
                     GGNTVGIEAAAWRYFERSPEQLSWAENAMLAVLPNSPSLINTAKKREILKEKRDFLLK
                     KLYTKNIISEEEYELAILEPIPEHPNPYPMHAYHLVNRAAKEFPNNNSKIETTIDINL
                     QKQINEIVNIYNQRYKANHIYNIACLVLEVESGKTLAYVGNADINSETPEKAIDMIKA
                     NRSSGSILKPFLYAAALSAGEILPKTLLRDTPLRIGSFSPENFDKSFDGAVPASEALA
                     RSLNIPFVLLLKEYGIAKFIYVLKSLGLKSVNKSSEHYGLSLILGGAETSLWDICSAY
                     ASMARSLKNYNKYQSKYSKSDYHEASYIKNAELNEQEDLRDWSFLSASSIWFTFEAMS
                     SVNRPAEEKQWRTFSSKQKISWKTGTSFGFKDAWSVAVTPDYVVGVWVGNASGEGRNG
                     ITGLRVAAPVLFDIINILPSYKNWFEIPYDDMILAPVCKQSGYLAGENCCDIDSVWIS
                     TRGINTNICPYHQKIYLDQSETFRVNSDCYPVNLMKEKSYFILPPSMEAYFINSNYWY
                     QSLPPFLEDCSQQLNVSEQIELIYPTNLSRVLIPIEMTGDTLQAIFKVSHRNPETKVY
                     WYIDEKYYGFTKDFHEMGFKLKPGNYTLNLMDENGYKISKKFKVISND"
     gene            complement(6010..7221)
                     /locus_tag="PKN53_04030"
     CDS             complement(6010..7221)
                     /locus_tag="PKN53_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88153.1"
                     /translation="MNINQVIAIIGAIPLFAVRTFFPAFLTALFLAHPEWFSFINYES
                     EIVAESSFWAQHWLVIVLGILSILEFISDKNSSLRIFLHEAEPYMKPIAYLLIQIFAL
                     SQQNTEVINAIQWSGFNPVIIIGIIGAFAVYVLAKIRKTTLDWLKNFDLDDNLKIGKL
                     ISWIEDSFVVFGFILLMVAGIFMIILYLIIIAILIFLRHKYKKRSEKAKIECPNCKNK
                     IYSFATKCQYCNFEISEPHKIGFLGNRKNAKTKDIKTHKLHLLSQSRCPNCASIIKKQ
                     KSTPICSECQTKLFDNPSLDEFIKFQDLRFYKILAFSFLAGFIPLFGFIVSAVLTDII
                     LLAPYRRYLSNINNFFIKILYRLIFIILVFLGIALGFIAAPLYCGIKYLIVKDRFVKK
                     FNSNNEVLKTL"
     gene            complement(7226..8485)
                     /locus_tag="PKN53_04035"
     CDS             complement(7226..8485)
                     /locus_tag="PKN53_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HOD88154.1"
                     /translation="MKKLILLILIISINISNSFSAFVLIPMDDTQTNHLKAYGLTYWT
                     LQNQVEAWWLLNYRGGSFLLNFHSEIERECILRGVSYEIIADAQKNAIFNEISSPSVN
                     MDAVKLEVAPKIAVYTPPFSQPWDDAVTLALTYAEIPYDKIYDREIINGELGKYDWLH
                     LHHEDFTGQYGKFYGAYGRMQWYRDNQKASEAEALSMGFNKVSQLKLEVAKTIKAYVA
                     EGGFLFAMCSATDSFDIALAAEGVDICETMFDGDPADPNAQSKLDYSKCLAFKDFTLV
                     RNPFEYEYSDIDVTDKRKVAQNEDFFVLFEFSAKWDPVPTMLCQNHTSMVKGFMGQTT
                     AFKAELVKPSVLVMGENKAANEIRYIHSEFGKGMWTFYGGHDPEDYRHFVGDEHTDLN
                     LYPNSPGYRLILNNVLFPAAKKKKLKT"
     gene            8853..9737
                     /locus_tag="PKN53_04040"
     CDS             8853..9737
                     /locus_tag="PKN53_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOD88155.1"
                     /translation="MERQTKANLYALATVLCWATVGTAFKIALRFVNYVQLLFWSVLV
                     SLIVFAIILSIKKLWQKLLEQSKTDILKSAIIGFISPFVYYLVLFQAYDLLPAQEALT
                     VNYMWPIVLVLLSIPILKQKISFLNILSIFISFLGCFIIVSKGKVFDLEFSNSFGVFL
                     AILSTFIWSIYWLFNVKDKRNEIVKLFMSFLFGFLFISLYSIFTDNLILPERNGLLAV
                     TYVGIFEMGLTFFLWIMALSLSKTTAKVGNFIYITPFLSLMIVSLVLKEKIHIATIFG
                     LVLIVLGIVMQKQSKVKS"
     gene            complement(10200..10742)
                     /locus_tag="PKN53_04045"
     CDS             complement(10200..10742)
                     /locus_tag="PKN53_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOD88156.1"
                     /translation="MTEEIIIAGFGGQGVLSMGKILGYSGISQGQEVSWMPSYGPEMR
                     GGTSNVTVIISDERISSPILAKYDTAIILNQQSMDKFEATVKPGGTLLYDPNGISTPP
                     TRTDINIYKVDATNIASELGNAKVFNMIMLGAYLKIKPIVKMENVIAGLKLSIPERNH
                     HLLPLNQQAIDKGMEAVKKA"
     gene            complement(10754..11533)
                     /locus_tag="PKN53_04050"
     CDS             complement(10754..11533)
                     /locus_tag="PKN53_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOD88157.1"
                     /translation="MTLKDIIKEENLVYKRSEIMTGKTLSYCPGCGHGTAHQIIAHTI
                     QDMGIQEQTIGISPVGCSVLMYDFLDLDIQQAAHGRAPALATAIKRVMPEKFVFTYQG
                     DGDLAAIGTAETIHACNRGENITIIFINNGIYGMTGGQMAPTTLEGMPTATCPYGRDT
                     SIMGNPLKITELLALLPGTYYVTRQAVHTPRAVRQTKKAIRTAFENQKLNRGVSFVEI
                     VSNCNSGWKLPPVKANEWMVENMFPFYPIGDMKVDGKIVNK"
     gene            complement(11546..12631)
                     /locus_tag="PKN53_04055"
     CDS             complement(11546..12631)
                     /locus_tag="PKN53_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOD88158.1"
                     /translation="MKKELRLMKGNEAVAEAAIRSGADAYFGYPITPQSEVLEYLMAE
                     KPYERTGMVVLQAESEVSAINMVYGAAGCGRMVMTSSSSPGISLKQEGISYMTGAELP
                     CLILNVVRGGPGLGTIQPSQSDYFQATKGGGHGDYRMIVLAPASVQEMYDFVELAFDL
                     AFKYRTPTMILSDGIIGQMMEKLEIGPQKPRRTPEEIAKQCPWATVGKPKNRERNIIT
                     SLDLSAVRQEEKNLKLQAKYKEIREKEVRYELINCEDADYILVAYGSSARLSMKAMDI
                     LRAKGVKVGILRLITLFPFPTKIIKEITPKVKGFLAVEMSCGQMVEDLRLAVEFKKPV
                     EHFGRVGGMIPSPEEIVEALEQKIIGG"
     gene            complement(12655..12882)
                     /locus_tag="PKN53_04060"
     CDS             complement(12655..12882)
                     /locus_tag="PKN53_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOD88159.1"
                     /translation="MAKIRGAVVVDIEGCKGCGVCTVACPAKVLALAKEVNGKGYNYA
                     YMQNPEACIGCESCAIVCPDGVITVYKVKVD"
     gene            complement(13104..14552)
                     /locus_tag="PKN53_04065"
     CDS             complement(13104..14552)
                     /locus_tag="PKN53_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD88160.1"
                     /translation="MREKFFNKQVSEYIKHNPTTDQSTAIEEINKFLNDKEKYCVFLL
                     TGYAGTGKTTLISALINTLNDYKVKTVLMAPTGRAAKVLTNYSGKPAYTIHKCIYRKK
                     SNKSNESKFNLNFNNHKDTIFIIDEASMISNQDFSESIFGSGKLLEDLLSFVYNDNNC
                     RLILVGDSAQLPPIGTNLSPALDDKYLYSYGMKVYKSELCEVVRQDQDSGILKNATDL
                     RLNLQNQNISLPKFQTTFPDVEYITGADLLEELESCYSKYGEDETKVVCRSNKTASQY
                     NLGIRNRILWKEEGISHGDKIMVVKNNYSWLPENEKIDFIANGDILEVKRIGRTQEIY
                     GYRYIDLSLQFVDYPELEIDVKAILDCLSSEKPAMDDNYYKNLYQLLQEDYQDITDSQ
                     KRHEKILEDPFFNALQIKFAYALTCHKAQGGQWKSVFIDHGYINFNELSEEGVHSLNR
                     WFYTGITRATEKVYLVNFAKILLEKYESIKNY"
     gene            14634..15548
                     /locus_tag="PKN53_04070"
     CDS             14634..15548
                     /locus_tag="PKN53_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88161.1"
                     /translation="MNKIVGIILKVVLLALCIVLGYYIVAGIKKPIDFESERERRFEM
                     TKQRLIDIRTAQVAYRAWYGEFTPSFDSLINFINHGHLRVIYSEGEVPEDKTESQALK
                     DGDITRDTILVPVKDSLFKHIKYDVKDIVYIPCGNKAKFTMDTATVLTGSGVNVKVFE
                     ARCSYWNILDGLDEQLIVNYNAAKLQRAHVDIYLNGNILKDNLSLTNGKGPLNYKFEA
                     KKGDILSINYTSGAWSHENLVNLIGPDKKYILKEHNPNPLKPWESKPLEQDGEYIIQL
                     LDTYGDGWYGSMAVGSLTEANNNAGNWE"
     gene            15553..16398
                     /locus_tag="PKN53_04075"
     CDS             15553..16398
                     /locus_tag="PKN53_04075"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HOD88162.1"
                     /translation="MISVNIIDNNTEKVLEKAKLELSILFTENGLSYFIFYRDENKIL
                     GLKSFEFKKNEDYYNQAASILSEIIEDENPYDLVRFVVADNKQTIVPEAIFDENNIET
                     YWQLNFETQSQTKLMHHYLNRAKSFIVFSVNESIETLFNNYRGKKTIIPSSASFIDFN
                     LKRNKLVENPELERVYVQVYENYMEVILLQNHNLRLFNTFTYKSQNDLLYYIINLYEQ
                     LKISQTDSELILSGFVETNSLLALNLKKFIKIVYFEARNTEYNYFYKFQDIAPHYYFY
                     FLNIS"
     gene            16395..16928
                     /gene="rsmD"
                     /locus_tag="PKN53_04080"
     CDS             16395..16928
                     /gene="rsmD"
                     /locus_tag="PKN53_04080"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOD88163.1"
                     /translation="MRIIGGKYRGKVFNPPTNFAARPTTDFAKEALFNVLNNIYNFEN
                     LEVLDLFSGTGSISYEFLSRGSKVVTAVENNQKHVNYILKQFEILKADQANVIKIDAF
                     KYLANCKRQFDIIFADPPYEDKNIIEIYNLVYEKSLLKEDSLLIIEHSGIYDFSQLSK
                     FKEMRHYGSVYFSFFET"
     gene            complement(16930..17436)
                     /locus_tag="PKN53_04085"
     CDS             complement(16930..17436)
                     /locus_tag="PKN53_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HOD88164.1"
                     /translation="MDIFLVIIGFICILIGIIGCIVPVLPGPPLSYVGLLLLQFSKYG
                     NFTTATMIILALAVIIITILDYVVPSWGTKKAGGTKWGVRGSAIGLIIGLFFAPAGIF
                     IGPFVGAFLGEILYRQYQNKKSKDNFSKSLKAAFGSFLGLIFGIALKIAISCFIAFLF
                     VKEFIINL"
     gene            complement(17436..17867)
                     /locus_tag="PKN53_04090"
     CDS             complement(17436..17867)
                     /locus_tag="PKN53_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88165.1"
                     /translation="MKKNIIIYIFLILFISSCTLIDKKLCRNFYTTDECFRASSVATS
                     SDRQLSSEKALLIAKKEIALEVDTYILSKFSHQTFLADPEFENKLTVARKTILNDITI
                     ICSLTTHNRRKNIYKTRMSIEMSKSSIDETVSKNLKATDIN"
     gene            complement(17892..18644)
                     /locus_tag="PKN53_04095"
     CDS             complement(17892..18644)
                     /locus_tag="PKN53_04095"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD88166.1"
                     /translation="MKKIVLINIGILLCTAFYANNINTLQDSALNNYINKDYEKALYF
                     YDSISKLGYSSADLHFNLANCYYNLGNIAEAIYNYEKANVLAPNDKDISHNLNIARKS
                     VRTKTEQKPEVFYVKWFNSILDLFSSNAWLYISITAFVISLIMTGLYFFSTKLGLRKT
                     GFYLGILLLIFSIICLIFSRLQANKILNNKFAIVFESSVIKSSPSEEGTNLFEISEGM
                     KVEVLDELQEWSEIKLDDGKEGWLLTESIRKL"
     gene            complement(18646..20475)
                     /locus_tag="PKN53_04100"
     CDS             complement(18646..20475)
                     /locus_tag="PKN53_04100"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOD88167.1"
                     /translation="MRRLLLICFTIFLHFGLVAQDVSFVASAPSKVGVHQNFQVKYTV
                     NAKGSNLNLGNYSGLKLVNGPFSSSSTSTQIYNGQVTTNVSYTYTYTFQASNTGKFTI
                     SGASINVDGQQYTSNSVTIEVQKDPVQTQQSRQQTYYDPFEDFFNLGRSRTQPQQPKE
                     ITNEDLFIRVIPNKTNVYKGEALTVSIKLYTRVDLSGLTDLEFPSFDSFYVEELESAT
                     RLLFNRENYNGTVYDVALIKTYLLYPRVTGKVVIESCKAECNVRQYVGGGFFARYQDI
                     PRKLQSPEISVNIKNLPNPPENFTGAIGNFNIELVQSEDTVNVNDAVSFKFVVKGTGN
                     FNMLEEPKIVWPKEFEIYDPVVSQNLKATNAGMNGSKTWEYTIIPRYPGKFNLGQISF
                     VYFDLNSHQYKILKTQDIKLAVRKDIYDTKFENDYNYSQKTVEFIDDDDIRFVKRGNL
                     NLVKNFTPSVKSCIVWLFYLLPLAIFITISILLRKRIKENANIAARKVKLAGKTSRKR
                     LKKARKFIQQNKKNEFFKEIISALWGYTSDRFGIPIAELTKDKVITVLQALNIEEELI
                     NKFIHLIDKCEYAHFAPSSEETSFSTIYSEAVEVIEKLEQKIR"
     gene            20652..23390
                     /locus_tag="PKN53_04105"
     CDS             20652..23390
                     /locus_tag="PKN53_04105"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD88168.1"
                     /translation="MKRDSILALAELFAIVANKNLKIDNKLYLDVFTNFLTSVVEESY
                     VKDILKFFSDNLAESATGDNLKKLSLNSVKLIAKLEKLKSSLSSCERYFVFLHLIILN
                     NKTGYTEYSEAFLDEVAHSFEIEQDLKTKIRTFIENNEENRELADSILFVHDELILFL
                     PSIDTYLHNGKKLAKNEVCVLDKDSIVTDFEGKNYFFSEFRALCGNSEENEKFSLVVK
                     NLEYKIKNKTLVQKTSVKFDSSQLIGIVGNSGSGKTSFLKCLAGFETKYSGEVGFVSD
                     VDKNFNLAFVQQENSFVPKLSVEEHLYERAKFLQYDKQKSHEKINEILEFVNISEQRD
                     NLVCKTDFSLDQISGGQQKRLAIATELLVNPSVLLLDEPTSGLSSEDAYDIMAKLKQL
                     TKQGKLVIASIHQPELDLFLMFDKILVFDDGGYTVYFGKPQDAVEYVRKIDERIDRKT
                     VFTLSKRPSILLNLLKENKAKNNKESLKHKLYSIFINENKSLEDLDLKTNVQTNRLNG
                     LRSFLYYLRFGFFVDFKHKLRLLLMLVLPLIAGVLLSLVTRYSYGETYQYFYNPNIPV
                     WLLIVLTIAVFVGLVSSGHEFIYLRKYINNKYKISPNYLPHVLAVSLRYVIYSFIQTL
                     ALVLPSVFIVGAGFHFADMFLFSFLMCVWGSLFGLLLSYLLKTNLMVYLLIPLIIIPQ
                     LVYSGALIRFSEFNKNKNSEREIPVVASLIPMRWAAEGVIYNLYAENPYYSKLYENRC
                     ELYEASYFADIYVPELMELKKTDSLQVYEILKNEQIEFIENFVNIDNSLSNIEQYYKV
                     IKQNCLQKEDSIITAMPNGSEAKLKYSNQAISNIVNYVNKGNFIAIKNAKTCRNFKNI
                     YSNNNLQIENEKYMVGSKSLCNITLKTGQFNLLVLIALDVFLFFLLVVVATLKRSR"
     gene            23526..26519
                     /locus_tag="PKN53_04110"
     CDS             23526..26519
                     /locus_tag="PKN53_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD88169.1"
                     /translation="MRKIFILFLALTFIFGGLANAQQLKSIAKAKASVNAVANTNAEI
                     GAFKMNGEKPAEKPATGKQVKLNSLLGSGAKATTVYSEDFTGGMGGWTVVGLGDGSWS
                     VKDESNAGGTAPELMLEWVSGSDFDGQSYCMSPVINTTGYTTLALEFKQWFRNYQGSS
                     VLGVYTTIDDGATWEEVWSETVSANFVEVKNLLITNAHVGNANFRIAFGFNGNTFDIW
                     EWAIDDILLVEPDAHDLAIVAINLNTFVESGSTVTPQVTIRNMGISNEATWSLTLTDG
                     DAYTSTKSDILINIGDNLVVDMDDWTPADGQYTLTATLTLTDDANTSNNTLTATVDVI
                     EFSTAFAWNAYHEGGLNATGEGPVNIVLQTGIMTQIAEDDSDFIMAADYVEDEIYGIR
                     YADGVPRPLVKIDPVTGVVTNIGGGAVDLVGFTYDVTTETAYVIDFNGMLGTINLETG
                     EVTNIGGDYTSVIGLACDTSGNLIAISLADHIASIDKTTGTSTIIGNLGVDINYAQDI
                     CYDRDNNILYGALYTDQGAIYIINTTTGAATLVSEVIDELTGFAIPYTLVGPQVVLLT
                     PEANAVGVALNAEVSVTFDVDLTAVDLTGITITPDPGNVSASVNNAVLTIAHDNFNYN
                     TEYTVLVPAGAVSDGTDPTTHPITWSFKTVLDPTACNEPTGIVFSDIQAEQATVAWTE
                     TGAGTSWRVKYGATGFDPDTEGEEVVVNEQTATITELTGNTTYEVYVQALCGTDETSG
                     WSGSAIFATACGAITTLPYLEEFEGAFPTECWTFVNNHADPTSNWHIDNYQDTGDPAL
                     NILWSDENMMDEWAISPVFDLSGITGEIALTFDFLMSYYYQVDPTNGADVMVKVTTDN
                     GTTWTKIWQEEDYGTFENWTWYTATLPFSTYVGESNVKFAFHYLGNDGAQVMIDNVEL
                     DYATSVNDNVTNLVSVYPNPSNGIVNVQVSETSVITIVDLAGRVVATYNANANETLSF
                     TQAAGVYVVKVESNGQVSNHKLIIQ"
BASE COUNT         8853 a         3837 c         4292 g         9647 t
ORIGIN      
        1 attatttata cgttaaattt ttaattttgt tgtcctaaaa aaggggacca ttatactatt
       61 ctgctgataa tttttttaat ttattatcct gagaataaaa atagagcaac aattttctca
      121 ttaatactat ttaatgttgt aattttttta ctaacttttg tacttaatga tataaaaata
      181 tccatgggag ctgcttttgg gttatttgct gtcttttcaa tgcttagata tagaacagct
      241 ggaatatcat tgaaagatat gacttatctg tttattttca ttgctttggg tttggtaggc
      301 gcaattggat tgaaagttca agatttggca ataattaatg gtataatatt tatttttatt
      361 tttgttttag atggtggctt tatttttaaa agagaatatt cgcagatagt tgtttatgaa
      421 aaaatagaaa atattaaagt cgaaaattat gatatattat tagaggattt aaaagaaaga
      481 acaggcttaa atattcaccg aatagctata ggaaaattaa actatctgag agattctgct
      541 caaattaaag tcttttatta tcaaaaaaat aaaaaagtat atccttacgg tgaagaaaaa
      601 taaatttgta ttacactaat tcgcttgatt tattttcttc caatgattaa tgattgaatc
      661 taatttgagt ataagatttt tgtgagtttg ctttagtttt ttgtattctg atttgttgaa
      721 gtatcttaat tttaataatc tccaatcctc aactaaaatt attatccatt caaaaattaa
      781 ttgagctaat aagcctgtta gcggtagaga aatagcatat gcaatgatta gataaatatt
      841 tggaataact agtgaaaaaa ttacaatttg aattaaataa attattggag ctaaaaatac
      901 cgcccaaaca aactttatac tagagtgaaa ttgcttgtct ttaattttta aaactataag
      961 ttttggactg aaatatacca aagcattgtt aagcaaacca tatatataaa taggtaaaca
     1021 taaaataata atcaaagtat ttattattaa tctaaaaatg cttaaaaatg gtttttcaat
     1081 agattggtcg ctgagtttat atttcttttt taggctttca tgatgctcaa cttgagattt
     1141 tatttctagc attttttctg gatagtcgca ttcaaaagta tttaatgcgt ttatagttat
     1201 tttgtctgct ttaaacttat ttatttgtgt tagttttcca agctgaaatt tcctaattaa
     1261 attttttacg tacaaagttc taatgctttc gtaagtgtcg tacaattcta agttttgaat
     1321 atcaattgtt aaattgctag ttgcctcttg cattttgtct cgcaagctaa gcatagcttt
     1381 ttgctgattg tcaaaatatt catctttaaa atcagaaaca gaaattggtt ttccatatct
     1441 tatgtgaatt atacttcgca ttctgttgta ttgactgtaa taaattccaa caggaataat
     1501 ttttagattt aagttgaaat tatttttttc ttcagccata aatgcaaggc gaggaattcc
     1561 ttttttgaag tttaaaagtt gtcttttatt attgtggcgt gcttctggaa aaattccaat
     1621 atattgtttg ctttctaaaa cttttactgc aatatcaaaa gttaaatcat tgtttgctaa
     1681 attttctttt ccatctctaa tgcgaaagac aggtaaaatt tttaagaaat ataaagcttt
     1741 agcaaaaatt ttatttttaa aaatatcggc tctagccaaa aaaacaattt gtctattaaa
     1801 tgtaaacaga atagcaagcg ggtccattaa agcattttgg tggttaggcg caaaaattaa
     1861 aggacagtct ttaggaatgt tttctttgcc ttcaactaca atgtttttgt aaaataaggt
     1921 atgtagcgtt ttaacatata gaccaaagaa attccaccaa aacgattgtt tatataaatg
     1981 tttatatttt tctatactgt ctttcttcgc catattgttg aaatagttag tcctgaaatt
     2041 atatgccaaa ttccccacca tgcagtaact atcatcattc cgccaagtgc taggtcttga
     2101 gggaaaatat ttggattgaa taatagaact aaacctaagc cactattttg tattcctgtt
     2161 tctatcgcaa ttgttcgaga gtttatagta tttaatctga aaagtttagc aaatccataa
     2221 ccagagctta aagcaagagc attatgaatt aatacaacaa caaaaatcca ataaatatat
     2281 tttacaaaat ggtcataatt atttgcaaac attataacga ccattcccat aaaaaagaaa
     2341 agcgaaaaaa gtttaatagg tttttttatt ttttctgtaa actttggaaa tttgtggttt
     2401 actaccattc ctaaaacaat tggaatacct aataaaataa taaccgtttt aagcatttct
     2461 attgggttta tagtcagttc gcgtagtaaa gcattgtcaa cccaaggtcc aaatgtttta
     2521 tataatttcc cataaaaata gaaattaaac ggcgttaaaa ctattgctag catagttgca
     2581 attgctgtta aagatacaga aagtgctgcg ttcccttttg catgagttga caaaaagttt
     2641 gatacattcc cgcccggaca tgctgcaact aatatcattc ccattgcaac gcctgctgtt
     2701 aaattttccc aaaatgccat tactaataaa aaagtaagca aaggaagtac taaaaattga
     2761 gacaaaaatc caacaattac tggttttggt tttttaaata attctttaaa gtgcgaaact
     2821 ttaatttcga gggcaacacc aaacataatt accgctattg caatatttaa gacaagtaaa
     2881 ccaccgtcag aaaaatttag tgttgctccg tccatttttt gtaaggcttc aaacatattt
     2941 attacaattt tgttacaaaa ataataatgt ttatgtattt ttttggattt ttatgcttaa
     3001 tttgcatata aatcttgtta attttaataa aattgcttaa ttttaaaaat taaaattttg
     3061 aaagatgaag aatcacggaa ttaatattcg ctataaggaa tttagttatg gtgaattaga
     3121 tagcgttgat aaaaagttgt tagattcggc tgttgaagct gcaaaaaatg cttatgctcc
     3181 atattcccat tttagagttg gagctgctgt cttacttgat gatggaagta tcataacagg
     3241 gaataatcag gaaaatgctg cttatccttc tggtttgtgt gccgaaagag tggctttgtt
     3301 ttatgcaaat tctaaatatc cgcaaaaaac agtagttgcc gtagctatag tggctgtaaa
     3361 ccccgaaggt gaagttttaa aaggaaatgt ttctccatgc ggttcttgtc gccaagtaat
     3421 gctcgaaact caaacgagat ataaaaattt aataaaaatt atgttagctt cagatgacgc
     3481 agttttagtt tttgcttcgg ttgaagattt gttgcctctt aattttagcc aagacagttt
     3541 gaaggggaat tgagaattgt gtctcgacta cgctcgacac ccgttcggct ctgctcgaca
     3601 cccgatgatt gaattaataa ttttagaatt tttagtcgtt gcttattact ttgaattttt
     3661 ttgatatttt gtatccgttt tcatccataa ggtttaaagt gtaatttcca ggttttaatt
     3721 tgaagcccat ttcatgaaaa tcttttgtaa atccataata tttttcgtct atataccaat
     3781 aaacttttgt ttctggattt ctgtggctta ctttgaaaat agcctgtaaa gtgtcgcctg
     3841 tcatttcaat agggattaat accctagaca aatttgttgg ataaataagt tcaatttgct
     3901 cgcttacatt taattgttgt gaacaatcct ctaaaaaagg tggtaaactt tgataccaat
     3961 aattggaatt gataaaatat gcttccatag aaggtggtaa aataaaatat gatttttctt
     4021 tcatcaaatt tacaggataa caatctgagt ttactctaaa tgtttcgctt tggtctaaat
     4081 aaattttttg atgatatgga caaatattag tatttattcc tcttgtgctt atccaaacgc
     4141 tatctatatc gcaacagttt tcgcctgcta aatatccgct ttgtttacaa acaggagcta
     4201 aaatcatatc atcgtatgga atttcaaacc aatttttgta agaaggtaaa atatttataa
     4261 tatcgaataa tactggtgcg gcgactctta gacctgtaat tccgtttctg ccttctccac
     4321 tagcatttcc tacccacacg ccaactacat aatctggagt tacagcaacc gaccaagcat
     4381 ctttaaagcc aaaactggtt cctgttttcc acgaaatttt ttgctttgat gaaaatgttc
     4441 gccattgttt ttcttctgcg ggtctattaa cagagctcat agcttcaaaa gtaaaccaaa
     4501 ttgacgaagc cgataaaaat gaccaatctc ttaaatcttc ttgctcgttt aattcagcat
     4561 tttttatata gcttgcttcg tggtaatcag actttgaata tttggattga tatttattgt
     4621 aattttttaa ggatcgtgcc atagaagcat aagccgaaca aatatcccat agcgaagttt
     4681 ctgcacctcc taaaattaaa gacaaaccat aatgctcaga gcttttattt acagatttaa
     4741 gacctaatga ttttaaaaca taaataaatt ttgctattcc atattctttt aataagagta
     4801 caaaaggaat atttaaagac cgtgcaagag cttcgcttgc aggaacagct ccatcaaaac
     4861 ttttgtcaaa attttcggga gaaaagcttc caattcttag tggagtgtct cttaataatg
     4921 ttttaggtaa aatttcgcca gccgataagg ctgctgcata taaaaaaggc tttaaaatac
     4981 tgccagaact tctgtttgct tttatcatat ctattgcttt ttctggagtt tcagaattaa
     5041 tgtcagcatt tccaacataa gctaatgttt ttccgctttc tacttctaaa actagacaag
     5101 caatattata aatatgattg gctttatatc tttgattgta aatatttact atttcattaa
     5161 tttgtttttg caaatttata tcaatagtgg tttcaatttt ggaattgttg tttggaaatt
     5221 cttttgcagc tctatttact aaatgataag catgcatagg ataggggtta gggtgttctg
     5281 gaattggctc caatattgct aattcatatt cttcttcgct aataatattt ttagtgtaaa
     5341 gttttttcaa taaaaaatct cttttttctt taagaatttc tctctttttt gcagtattta
     5401 ttagtgatgg cgaatttgga agtactgcca acattgcatt ttctgcccag gaaagttgtt
     5461 caggtgagcg ttcaaaataa cgccatgccg ctgcctctat tccaacagtg tttccaccaa
     5521 aaggtgcatg agaagcataa agttgaagaa tttctttttt tgaatatgat aactctaaac
     5581 gaaatgctaa aataacttca attatttttt ggtaataagt tctttctttg ccttttctag
     5641 caatacgaat tgtttgcatg cttatggtcg aaccgccact tttaattttt cctgaactaa
     5701 tattttgttt tatcgctcta gtaatagcaa ttgggtctat gccaaaatga tagtaaaatc
     5761 tttggtcttc aaagtttaaa atgcaggctt caaatttagg acttattttt tgagtttcgg
     5821 gaaatcgcca ctgcccatct tctgcaattt ttacacctaa caattgattg tttttgtcat
     5881 aaacagctgt ggaatacgga tctttaaaaa gtgttgatgg taaggaaaaa ataaacacta
     5941 atattatggc aaaccaaata gtaattgaaa taattagtaa tattttggct tttttcataa
     6001 tcttgtttct tataatgttt ttaaaacctc gttgtttgaa ttaaattttt tgacaaatct
     6061 gtcttttact ataagatatt taattccgca ataaagtggt gctgcaataa atccgagagc
     6121 aattcctaga aaaactagta taataaaaat tagcctataa agaattttga tgaaaaaatt
     6181 attaatattt gataaatatc ttctgtaagg agctaataat attatgtcgg taagcacagc
     6241 gcttacaata aatccaaata gtggaataaa tcctgctaga aatgaaaaag ctaaaatttt
     6301 gtaaaatctt aaatcttgaa attttataaa ttcatctaaa cttgggttgt caaataattt
     6361 tgtttgacat tcagagcaga tgggcgtgct tttttgtttt ttgataatac ttgcacagtt
     6421 tggacagcgt gattgactta aaaggtgtag tttatgcgtt tttatgtctt tagttttggc
     6481 gttttttcta ttccctaaaa atccaatttt atgtggttct gaaatttcaa aattgcagta
     6541 ttggcattta gtagcgaagg agtatatttt atttttacaa tttggacatt ctattttagc
     6601 tttttcactt cttttcttgt atttgtggcg aagaaaaatt agaatagcaa ttatgataag
     6661 gtataaaatt atcataaaaa ttcctgctac cataagtaaa ataaatccaa ataccacaaa
     6721 actatcttct atccagctaa taagttttcc aattttaaga ttatcatcaa ggtcgaaatt
     6781 tttgagccaa tcaagagttg tttttctaat ttttgcaaga acatacacag caaaagcacc
     6841 aataattcct attataatca caggattaaa gccagaccat tgaattgcgt taattacttc
     6901 tgtattttgt tgacttaaag caaagatttg aattagcaaa taagcaattg gtttcatata
     6961 tggttcagct tcgtgtagga aaattctaag gcttgaattt ttgtcggaaa taaattctaa
     7021 aatacttaat attcctaaaa ctattactaa ccaatgttga gcccagaacg aagattcagc
     7081 aacaatttcg gattcataat ttataaatga gaaccattcg ggatgagcca aaaataatgc
     7141 agttaaaaaa gctggaaaaa aagttcttac agcaaataaa ggaatggctc caattattgc
     7201 aataacttga tttatattca tatttctatg tttttagctt tttcttcttt gcagcaggga
     7261 acaatacatt atttaaaata agtctatatc caggtgagtt tgggtaaaga tttaagtcag
     7321 tatgttcatc accaacgaaa tgtctgtaat cttctgggtc gtgtccacca taaaaagtcc
     7381 acattccttt accaaattct gaatgtatgt atctaatttc attagctgct ttattttcgc
     7441 ccataaccaa cacagaaggt tttactaatt ctgccttaaa tgcagtagtt tgtcccataa
     7501 atcctttaac catacttgtg tgattttgac aaagcatagt tggcacagga tcccattttg
     7561 cggaaaactc aaataagaca aaaaaatctt cgttttgtgc aacttttctt ttatcggtaa
     7621 cgtctatatc ggaatattca tattcaaatg gattgcgaac aagtgtaaaa tctttaaaag
     7681 ctaaacattt tgaataatct aattttgatt gtgcgttagg gtcggcaggg tcgccatcaa
     7741 acattgtttc gcatatatca acaccttcgg cagcaagagc aatgtcgaaa gagtcggtgg
     7801 cagaacacat agcaaataaa aatccacctt cggcgacata ggctttaatg gtttttgcta
     7861 cttctaattt taattgagaa actttattaa atcccataga caatgcttct gcttcacttg
     7921 ctttttgatt atctctatac cattgcatac gcccataagc accataaaat tttccatact
     7981 gaccagtaaa atcttcgtgg tgtaaatgca accagtcgta ttttcccaat tcgccattga
     8041 taatttctct atcgtaaatt ttgtcgtaag gaatttctgc ataagttagt gctaaagtta
     8101 ctgcatcgtc ccaaggttga gaaaatggag gggtataaac agcgatttta ggagctactt
     8161 ctaattttac agcgtccata tttacagaag gagaggagat ttcgttaaaa atagcatttt
     8221 tttgtgcatc agcaattatt tcgtagctca caccgcgaag aatgcattct ctttcaattt
     8281 cggaatggaa atttaataaa aaagaaccac ctctataatt taataaccac catgcctcaa
     8341 cctgattttg taaagtccag taagtaagac cataggcttt taagtgatta gtttgagtgt
     8401 cgtccatagg aattaataca aaagccgaaa aggaatttga gatatttatg ctgattatta
     8461 atattaatag tataagtttt ttcatttctt tcaaatttga attatgcaaa gttacaaaat
     8521 ttataataat atctataaaa ttaaaaagag cccgagaaaa atctcgagct tcgtattaac
     8581 cttaatcaac catgaaaaag agaaaagtaa ctattattac ttttttaatt cattagttga
     8641 aactaaaata gtgcgatttt gttttgaaaa actgatgaat ttatactttt tgtgttaaat
     8701 ttaagaaaaa catatatctg tgttctatca aaatctaaaa aattattttg tttaagctac
     8761 aatttacttt tgttaaatct aaaattaaaa taaattgaaa atcgaataga tgttatattt
     8821 ttgtattaat tattataaat aggtatatat ttatggaaag acaaacaaaa gctaatcttt
     8881 atgcacttgc tactgtgctt tgttgggcaa ctgttggaac ggcttttaaa attgcattga
     8941 gatttgtaaa ttatgtacag cttttgtttt ggtcggtgtt ggtttcgtta attgtatttg
     9001 ccattatttt gtctattaaa aaattgtggc agaaattgct tgagcaatca aagacagata
     9061 ttttaaaatc agcaattata ggttttatta gtccttttgt ttattattta gttttgtttc
     9121 aagcctatga tttattacca gcacaagaag cattgacagt aaactatatg tggcccatag
     9181 ttttggtttt attatccatt ccaattttaa agcaaaaaat tagcttttta aatattttgt
     9241 caatttttat ttcatttttg ggatgtttta taattgttag taaaggaaaa gtctttgatt
     9301 tagaattttc taatagtttt ggtgtatttt tggcaattct cagtactttt atttggtcta
     9361 tttattggtt atttaacgta aaagataaac gcaacgaaat tgtaaaatta tttatgagtt
     9421 ttctttttgg atttttattt atatctctgt attctatttt tactgacaat ttaattttac
     9481 ctgaaagaaa cggtttgctt gcagttactt atgtcgggat ttttgagatg ggtttaactt
     9541 ttttcctttg gataatggct ttgtctttat ctaaaacaac agcaaaagtt ggtaatttta
     9601 tttatatcac tcctttttta tcgcttatga ttgtaagcct tgttttaaaa gaaaaaattc
     9661 atatagcaac aatttttggt ttggtgttga tagttttggg gattgttatg cagaaacagt
     9721 cgaaagttaa aagttaaaag ttaaaagtgg ctgtcgcgtc cacaaaagtt tagttaaacg
     9781 tttgtaattc aataaattat gcagtatggt gtagagacgg tgcatgcgcc gtctacacta
     9841 tacaaatgct aatttattgc acctcaaatc agttaaaact ggtattgtat tttagaaaat
     9901 acaaaatatt attgaaattg tattctcaag aataaaatcc tgcttgttcg gattttttca
     9961 cataaccccg ctcgctcgaa attttaatcc gagtgtaaca tttccaaaca ctcggattgc
    10021 aaatccgagc gagcgtcatg ggagttagga gcgagtgtca tgggagttag gagcgagcat
    10081 catgggtgta gagagtgagt agcataggta tagagtgaga gcgagcgtag caaaatttaa
    10141 cataaaaaaa agcaccaatt acggtgcttt tttatttttg tttaaaagtt tttaaaaagt
    10201 tatgcttttt tcacagcttc cataccttta tcaatagctt gttgatttag aggtaatagg
    10261 tggtggttac gttctgggat tgataatttt aatccagcta taacgttttc catttttact
    10321 ataggtttaa tttttagata tgcaccaagc ataatcatat taaacacttt agcattacct
    10381 aattcagatg ctatgttagt tgcatcaact ttataaatgt tgatgtctgt acgagttggt
    10441 ggagtagaaa taccgtttgg gtcgtataat aaagtaccgc ctggtttcac agttgcttca
    10501 aacttatcca ttgattgttg gttaagaatg atagctgtgt catattttgc taatatagga
    10561 gaactaattc tctcgtcgct aataataacg gtaacattag aagtaccacc acgcatttct
    10621 ggtccgtaag agggcatcca cgaaacttct tgtccttggc taattcctga atatccaaga
    10681 attttaccca ttgaaagaac tccttgtccg ccaaaacctg ctataataat ttcttcagtc
    10741 ataattttaa tttttattta ttaacaattt taccgtcaac tttcatatct ccaattggat
    10801 agaaagggaa catgttttca accatccatt cgtttgcttt tactggaggt aatttccaac
    10861 cagagttaca gttagaaact atttcaacaa aagaaacgcc acggtttaat ttttgatttt
    10921 caaaagctgt acgaattgct tttttagttt ggcgtactgc acgaggagta tgaactgctt
    10981 gacgagttac ataataagtt cctggaagca aagctaacaa ttcggtaatt tttagaggat
    11041 tacccattat tgaggtatcg cgtccataag ggcatgttgc agtaggcatt ccttctagag
    11101 tagtaggagc catttgtccg ccagtcatac cataaatacc gttatttata aaaatgattg
    11161 taatattttc gcctctattg cacgcatgaa ttgtttctgc agtaccaatt gcagctaagt
    11221 cgccgtctcc ttggtaagta aacacaaact tttcaggcat tactctttta attgcagtag
    11281 caagtgctgg agcacgtccg tgtgcagctt gttgaatgtc caaatcaaga aagtcataca
    11341 ttaatactga gcaacctaca ggtgaaatac caatagtttg ttcttgtatt cccatgtctt
    11401 gtattgtatg agcaataatt tgatgagcag taccatgacc gcaacctgga caatatgaca
    11461 aggtttttcc tgtcattatc tcgcttcttt tataaacaag attttcttct tttataatat
    11521 cttttaatgt gatttcagcc atgacttaac ctcctataat tttttgttct aaagcttcaa
    11581 caatttcttc tggtgatggt atcattccac caactcttcc aaaatgttca actggttttt
    11641 taaattcaac tgctaaacgt aagtcttcaa ccatttgtcc acaactcatt tctacagcta
    11701 agaatccttt tacttttgga gtaatttctt taataatttt tgttgggaat gggaaaagag
    11761 taattaaacg taagattcca actttcacgc ctttagctct taaaatatcc atagctttca
    11821 tacttaaacg agccgaagaa ccgtaagcaa caagtatata gtcagcgtct tcgcaattta
    11881 taagttcgta tcttacttct ttttctctta tttctttgta tttagcttgt agttttagat
    11941 ttttttcttc ttgacgaact gcagataagt ctagagatgt gataatatta cgttctctat
    12001 tttttggttt acctacagta gcccaaggac attgttttgc aatttcttcg ggtgtgcgtc
    12061 ttggtttttg tggaccaatt tctagttttt ccatcatttg tccaataatt ccgtctgata
    12121 aaatcattgt tggagtacga tatttaaaag ctaagtcgaa agcaagctct acaaagtcgt
    12181 acatttcttg aacagaagct ggagctaaaa ctatcatgcg ataatcaccg tgtccaccgc
    12241 cttttgttgc ttgaaaataa tctgattgag atggttgaat agttcctaaa ccgggtccac
    12301 ctctaactac atttaaaatt aaacaaggaa gttcggcacc tgtcatgtac gaaattcctt
    12361 cttgttttaa actaattcca ggactagatg atgatgtcat aaccattcta ccacaaccag
    12421 cagcaccgta aaccatattg atagcagaaa cttcgctttc tgcttgtaaa actaccatgc
    12481 cagttctttc atatggtttt tcagccatta aatattctaa cacttcagat tgtggagtaa
    12541 taggatagcc aaagtatgca tctgcccctg agcgtatagc agcttctgct accgcttcgt
    12601 tacctttcat taaccttaat tcttttttca tctttaaaaa tttttagtaa taaattagtc
    12661 cacctttacc ttataaacag taattacacc atctggacat acaatagcac aactctcaca
    12721 gcctatacag gcttcggggt tttgcatgta agcgtaattg taacccttac cattaacttc
    12781 tttagccaaa gctagtactt ttgcaggaca agctacagtg caaacaccac agcctttaca
    12841 tccttcaata tctactacta cggctcctct aatttttgcc atatctattt atatttataa
    12901 ttattaaata atattaaaat ttcttcaaag ttagaattat tttttatttt acaaaatgtt
    12961 tattgacagt tttaagagtt tttttgctta tttataatga aacaaaatta gaaaaattga
    13021 taaatcctct gtaaaatggc tgaatttcat aaatttaaaa gatttactaa ctaaaaaaag
    13081 aaaattatac ttatttaatt aatttaataa ttttttatcg attcatattt ttctaaaagt
    13141 atttttgcaa aatttactaa ataaactttt tcggttgctc ttgtaattcc agtgtaaaac
    13201 cagcgattta gtgaatgaac tccttcttcg ctaagttcat taaaatttat atagccgtgg
    13261 tcaataaata cagacttcca ttgtcctcct tgagctttgt ggcaggttag ggcataagca
    13321 aattttattt gaagagcatt gaaaaacgga tcttctaata ttttttcgtg tcgtttttga
    13381 ctgtctgtaa tatcttgata atcttcttgt agtagttggt aaagattttt gtagtaatta
    13441 tcgtccatag caggtttttc ggaggataga cagtctaaaa ttgctttaac atcgatttct
    13501 aattctggat aatcaacaaa ttgtaagctt aaatcaatat atctatatcc ataaatttct
    13561 tgtgttctgc caattcgttt cacttctaaa atatcgccat tagcaataaa atcgattttt
    13621 tcattttcgg gtaaccaaga ataattattt ttcacaacca taattttatc gccatgcgaa
    13681 attccttctt ctttccacaa aatccggttc cttataccta aattatattg tgatgccgtt
    13741 ttatttgaac gacaaactac tttagtttcg tcttcaccat attttgagta gcaggattct
    13801 aattcttcca aaagatcagc acctgtaata tattctacat caggaaatgt ggtttgaaat
    13861 tttggtaaac ttatattttg gttttgcaaa tttagtcgta aatctgtagc gttttttaaa
    13921 attcccgaat cttggtcttg acgcacaact tcacataatt cggatttata aactttcata
    13981 ccgtatgaat ataaatattt atcgtcaagt gctgggctta aatttgttcc aataggtggt
    14041 agttgagcag aatctccaac aagaataagt ctgcaattat tatcattata gacaaaacta
    14101 agtaaatctt ctaaaagttt gccacttcca aaaattgatt ctgaaaaatc ttgattacta
    14161 atcattgagg cttcgtctat aataaaaata gtgtctttgt gattattaaa gtttaaattg
    14221 aatttacttt cgtttgattt attagatttt ttgcgataaa tacatttatg tatagtatat
    14281 gctggcttac ccgaatagtt agttaatacc tttgcagctc tgcctgttgg agccattaaa
    14341 actgttttaa ctttatagtc gtttaatgtg ttaataagag ctgaaattaa agttgttttc
    14401 cctgttccag catagcctgt taataaaaaa acacaatatt tttctttgtc gtttaaaaat
    14461 ttgtttattt cctcaattgc agttgattgg tcagttgttg gattatgttt aatatattct
    14521 gaaacttgtt tgttgaaaaa cttttctctc atttttaaaa attagattga tttctatttt
    14581 tattttaaaa aaaataattt atttttgcaa aactaaaatt aaaattttta ataatgaata
    14641 aaattgttgg aattatttta aaagtggtat tacttgccct ttgtattgtt ttaggttatt
    14701 acatcgttgc tggaataaag aaacctattg attttgaatc agaaagagaa agacgttttg
    14761 aaatgacaaa acagcgttta atcgatatta gaacggctca agtagcatat agagcatggt
    14821 atggagaatt tactccttcg tttgatagtt taatcaactt tattaatcat gggcatttgc
    14881 gtgttattta ttcagaaggt gaagtgccag aagataaaac agaatctcaa gcattaaaag
    14941 atggtgatat aactagggac acaattcttg ttcctgttaa agattctttg tttaaacata
    15001 ttaagtatga tgttaaagac attgtttata taccttgcgg gaataaagca aaatttacaa
    15061 tggataccgc aacagtacta acaggttcag gtgttaatgt taaagttttt gaagcaagat
    15121 gttcttattg gaatatctta gatggtttag atgaacaact gattgttaac tataatgcag
    15181 ctaaattgca aagagcgcat gtggatatat atttgaatgg aaatatttta aaagacaatc
    15241 ttagcttaac taatggtaaa ggtcctctaa attataaatt tgaagcaaaa aaaggtgata
    15301 tactatcaat aaactatact tcaggtgctt ggtctcatga aaatttagtg aatttgatag
    15361 gtccagacaa aaaatatata ttaaaagagc ataatccaaa tcctctaaaa ccttgggaat
    15421 caaaaccatt agaacaagat ggcgaatata ttattcagct attagatact tatggagacg
    15481 gctggtatgg ctcaatggct gttggtagct taacagaagc taacaacaat gccggtaatt
    15541 gggaataagt aaatgattag tgttaatatt attgataata atacagaaaa agtattagaa
    15601 aaagcaaaat tagaattgtc cattcttttc acagagaatg gactttctta ttttatattc
    15661 tatagagatg aaaataaaat acttggatta aaatcttttg aatttaaaaa aaatgaagat
    15721 tattataacc aagcagcaag tattctctca gaaataattg aagatgaaaa tccttatgat
    15781 ttagttagat ttgttgttgc tgataataaa caaaccattg tgccagaagc aatttttgat
    15841 gaaaataata ttgaaacata ttggcaatta aactttgaaa cacagtcgca aacaaagcta
    15901 atgcaccatt atttgaatag agcaaaatct tttattgttt tttccgttaa tgaaagtata
    15961 gaaacactat ttaataatta tagaggtaaa aaaactataa ttccatcttc agcttcattt
    16021 atagatttta atcttaaaag aaataaacta gtcgaaaatc ctgaattaga gcgagtttat
    16081 gtgcaagttt atgagaatta tatggaagtg attttattgc aaaatcataa ccttagatta
    16141 tttaacactt ttacttataa aagccaaaat gatttattgt attatataat taatttatat
    16201 gagcaattaa aaatttctca aaccgattcg gagcttattc tatcaggttt tgtagaaact
    16261 aatagcttgt tggctttgaa tcttaaaaaa tttataaaaa tagtttattt cgaagcgcga
    16321 aatactgaat ataactactt ttataagttt caagatattg caccgcatta ttatttttat
    16381 tttttaaata tctcatgaga ataataggag gaaaatatag aggaaaagtt tttaatccac
    16441 caacaaattt tgcagctcgt ccaacaaccg attttgcaaa agaagcctta tttaatgttt
    16501 tgaataatat ttataatttt gaaaatttag aagtattaga tttgtttagc ggtacaggaa
    16561 gtatttctta tgagttttta agtcgtggaa gtaaagtagt tacagctgta gaaaataatc
    16621 aaaaacatgt aaattatatt ttgaaacagt ttgagatttt aaaggcagac caagctaatg
    16681 ttataaaaat tgatgcattc aaatatttag ctaattgtaa acgtcagttt gatataattt
    16741 ttgccgaccc accttatgaa gataaaaata ttatagaaat ttataattta gtttatgaaa
    16801 aatcactact aaaagaagac tctttgctaa taatagaaca ttctggaatt tacgattttt
    16861 ctcaactatc aaaatttaag gaaatgcgac actacggaag tgtgtatttt agtttttttg
    16921 aaacttaatt tataaattaa taataaactc cttaacaaat agaaaagcaa taaagcatga
    16981 tattgctatt ttcaaagcaa tcccaaaaat taatcctaga aaagagccaa atgcagcttt
    17041 tagtgatttt gaaaagttgt ctttagattt tttattttga tattgccggt ataatatttc
    17101 gcctaaaaaa gcacctacaa aaggtcctat aaaaattcct gcaggagcga aaaataaacc
    17161 aataatcaat cctattgcac tgcctctaac gccccatttt gtaccaccag cttttttagt
    17221 tccccagctc ggaacaacat aatctagaat agttattatt attacagcaa gtgcaagaat
    17281 aatcatagtg gcagtggtga aatttccata ttttgaaaat tgtaataata gtaaaccaac
    17341 ataactaagt ggcggaccag gtagaactgg cacaatgcaa ccaattattc caatcaaaat
    17401 acatataaaa ccaattataa caagaaatat gtccattaat taatatcagt agcttttaaa
    17461 tttttactca cagtttcatc tatgcttgat tttgacattt caatactcat tcttgtttta
    17521 tatatattct ttcttctgtt gtgggtggtt aaagaacaga ttattgtaat gtcatttaaa
    17581 attgtttttc gagcaactgt aagtttattt tcaaattctg ggtctgctaa aaaagtttga
    17641 tgactaaatt tgctcaaaat ataagtgtca acctctaatg caatttcttt ttttgcaatt
    17701 aatagagctt tttctgatga taattgtcta tcactgcttg tagcaacaga actagctcta
    17761 aaacattcgt cagtagtata aaaatttctg caaagttttt tgtctattaa tgtacaagaa
    17821 cttatgaata aaatcaaaaa gatatagatt ataatattct ttttcaattg tatttttaat
    17881 ttactgtgag cttataattt tctaatactt tcggttagta accagccttc tttgccatcg
    17941 tctaatttaa tttcgctcca ttcttgaagt tcgtctaaga cttcaacttt catgccttcg
    18001 ctaatttcaa ataaattagt tccttcttcc gaaggcgagg attttattac agaactttca
    18061 aaaactattg caaatttatt gtttaaaatt ttatttgctt gaagtcttga aaatattagg
    18121 catattatgc taaaaattaa taataatatt cctaaataaa aacctgtttt tcttagccca
    18181 agtttggttg aaaaaaagta taagccagtc attattaggc tgataacaaa ggctgtgatt
    18241 gatatgtata gccaagcgtt tgagctaaat aaatctaaga ttgaattaaa ccattttaca
    18301 taaaacactt cgggtttttg ctctgttttt gttctaacag attttctagc tatattcaaa
    18361 ttatgagaaa tatctttgtc gtttggagct agaacatttg ccttttcgta gttgtaaatt
    18421 gcttctgcaa tatttcctaa attataataa cagttggcta aattaaagtg taagtcagct
    18481 gaagaatagc ctagttttga tatagagtcg taaaaatata gagctttttc gtagtcttta
    18541 ttaatataat tgttgagtgc tgaatcttgt aatgtattta tattatttgc gtaaaaagct
    18601 gtacacaaca aaatgcctat atttattaaa acaatttttt tcatactaac ggattttttg
    18661 ttctaatttt tcaattactt caactgcttc gctataaatt gttgagaagc ttgtttcttc
    18721 agaagaagga gcaaagtgtg catattcgca tttgtcaatt aaatgaataa atttgttaat
    18781 gagctcttct tcaatgttta gggcttgcaa aactgtaata actttatctt tagtaagttc
    18841 tgctattgga attccaaacc tgtcgctggt gtagccccat aaagctgata taatttcttt
    18901 gaaaaattca tttttcttgt tttgctgtat aaatttacgt gcttttttca atctttttct
    18961 agaggtttta ccagctagtt ttacttttcg tgcagctata ttagcgtttt cttttattcg
    19021 ttttcgtaaa agtattgaaa ttgtaataaa tatagctaaa ggcaacaaat agaataacca
    19081 aacaatacaa gatttaacgc tgggtgtaaa attttttact aagtttaaat ttcctctttt
    19141 aacaaatcta atatcgtcat catcaataaa ttcgactgtt ttttgagaat aattataatc
    19201 gttttcaaat tttgtgtcgt atatatcttt tctaacagct aatttaatat cttgagtttt
    19261 taatatcttg tattggtgcg aattcaaatc aaaatatacg aaacttattt gtcctaaatt
    19321 gaattttcca ggataacgag gaataatagt atattcccaa gttttagagc cattcattcc
    19381 agcatttgtg gcttttaaat tttgtgaaac aacagggtcg taaatttcaa attctttggg
    19441 ccaaacaatt tttggttctt caagcatgtt aaaatttcca gttcctttta caacaaattt
    19501 aaaacttact gcatcgttaa cgtttacagt gtcttcgctt tgaacaagtt caatattgaa
    19561 attgcctatt gctccagtaa agttttcggg agggtttgga aggtttttaa tatttacact
    19621 aatctcaggc gattgtagtt tgcgaggaat atcttgatat cttgcgaaaa aaccaccacc
    19681 aacatattgt ctaacattac attcggcttt gcaagattca attacaactt tgcccgtaac
    19741 tcgtggataa agcaaataag tttttattaa agcaacatcg taaactgttc cattataatt
    19801 ttctctattg aagagcaatc ttgttgccga ctctaattct tctacataaa aagaatcgaa
    19861 agaaggaaat tctaaatctg ttaagccact taaatcaact ctagtataaa gtttaatgct
    19921 tactgtaaga gcttcgcctt tatatacatt tgttttatta ggaattactc ttataaataa
    19981 atcttcgtta gttatttctt tgggttgttg aggctgtgtt cgagagcgtc ctagattgaa
    20041 aaaatcttca aaggggtcgt aatatgtttg ttgcctagat tgttgagttt gaacagggtc
    20101 tttttgtact tcaatagtta cagaatttga tgtgtattgc tgtccgtcaa catttatact
    20161 agcacctgaa attgtgaatt tacctgtatt gctagcttga aaagtatatg tataggtata
    20221 agaaacatta gttgtaactt gaccattata tatttgtgtg cttgtactac ttgatgaaaa
    20281 aggaccattt acaagtttta aaccactata attacctaag tttaagtttg aacctttagc
    20341 atttactgta tattttactt ggaaattttg atgtactcca acttttgatg gtgcacttgc
    20401 aacaaaactt acatcttgag ctacaagacc aaagtgtaaa aatattgtaa aacaaattaa
    20461 aagtaatctt ctcatgtatt tcttaaaata acttgttggc aaaactataa aattctaact
    20521 aatattacaa tttttgtttt aatggaacgc aatttattta aaattattta attttcatct
    20581 taaaaaatag ttaataagca aaaaaaatac ttaattttaa actttaattt tattgtttcg
    20641 aatattaaaa tatgaagcgt gattctatat tagctttggc agaattgttt gcaattgttg
    20701 caaataagaa tttaaaaata gacaataagc tatatcttga tgtttttaca aattttttaa
    20761 cttctgtggt tgaagaaagc tatgtgaaag atatattaaa gtttttttct gataatttag
    20821 ccgaaagtgc aacaggcgac aatttaaaga aactcagtct taattctgtt aaattgattg
    20881 ctaaacttga aaaactaaaa tcttcactat caagttgtga gagatatttt gtgtttttgc
    20941 atttgattat tttgaataat aaaacaggat atactgagta ttcagaagca tttttagatg
    21001 aagttgcaca tagctttgaa atagaacaag atttaaaaac aaaaattaga acttttattg
    21061 aaaataatga ggaaaatcgg gagcttgcag attctatttt atttgttcat gacgagctaa
    21121 tattatttct tccttcaata gatacgtatt tacataatgg taaaaaactt gccaaaaatg
    21181 aagtatgtgt attagataaa gattctattg ttactgattt tgaaggtaaa aattacttct
    21241 tttctgaatt tagagcattg tgtgggaatt ctgaggaaaa tgaaaaattt agtttagttg
    21301 taaaaaattt agagtataaa attaagaata aaactttggt tcaaaaaact tctgtaaaat
    21361 ttgatagttc gcaattaatt ggaattgttg ggaatagcgg gagtgggaaa acaagttttt
    21421 tgaaatgttt ggctggtttt gaaacaaaat attctggcga agttggtttt gtaagcgatg
    21481 tggataaaaa ttttaatctt gcttttgtgc aacaagaaaa tagttttgta ccaaaactta
    21541 gcgttgaaga gcatttgtat gaaagagcaa aatttttaca atacgataaa caaaaatccc
    21601 atgaaaaaat aaacgaaata ttagaatttg taaatatttc agaacaaaga gataatttag
    21661 tttgtaaaac agatttttct ttagatcaaa tttcgggagg acaacaaaaa cgtttggcta
    21721 ttgctactga attgcttgta aatccaagtg ttttgcttct agatgaacca acaagcggac
    21781 tttcttcaga agacgcttat gatataatgg ctaaattgaa acaacttaca aagcaaggaa
    21841 aactcgtgat agcctcaatt catcaacctg aattggattt gtttttaatg tttgataaaa
    21901 tattagtttt tgatgacggc ggttatactg tgtattttgg aaaaccacaa gatgctgtcg
    21961 aatatgtaag aaaaattgat gaaagaattg atagaaaaac tgtttttaca ttgtcaaaac
    22021 gcccttcgat attgctgaat ttattgaaag agaataaagc aaaaaataat aaagaaagtc
    22081 taaaacataa actatattca atttttataa atgagaataa aagcttagaa gatttagatt
    22141 taaaaactaa tgtacaaaca aataggttga atggtttgag atcattttta tattatctcc
    22201 gctttggatt ttttgtagat tttaagcata aactaagatt gcttcttatg ttagttcttc
    22261 ctctgattgc aggagtgttg ttgtcgttag taacaagata ttcatatgga gaaacttatc
    22321 aatattttta caatccaaat attcctgtat ggctattgat tgttttaaca atagcagttt
    22381 ttgttggttt agtaagttct ggacatgaat ttatttattt gcgaaagtat attaataata
    22441 agtataaaat ttctccaaat tatttacctc atgttttagc tgtgagttta agatatgtga
    22501 tttactcttt tattcaaact ttagctttgg tactaccttc ggtatttatt gttggtgcag
    22561 gttttcattt tgccgacatg tttttatttt cttttttaat gtgcgtttgg ggtagtttgt
    22621 tcggcttgct tttatcttat ttattaaaaa ctaatttaat ggtttattta ttaataccat
    22681 taataataat accacaatta gtttattcag gagctttaat taggttttcg gaatttaata
    22741 aaaacaaaaa ctcagaacgc gaaattccag tagtagcaag tttgatacct atgaggtggg
    22801 cagccgaagg agtaatttat aatttgtatg ctgaaaatcc ttattattca aaactttatg
    22861 aaaataggtg cgaattgtac gaagctagtt attttgccga tatttatgtg ccagaattga
    22921 tggaacttaa aaaaacagat tctttgcaag tatatgaaat tttgaaaaat gaacaaatag
    22981 aatttattga aaattttgtg aacatagata attctttaag caacattgag cagtattata
    23041 aggtaattaa acaaaattgt ttgcaaaagg aagattctat aattacagca atgccaaatg
    23101 ggagtgaggc taagttgaaa tattctaatc aagcaattag caatattgtg aattatgtga
    23161 ataaaggaaa ttttatagca ataaaaaatg caaaaacttg tagaaatttt aaaaatattt
    23221 actcaaataa taatttgcaa atagaaaatg aaaaatatat ggtcgggagc aaaagtttat
    23281 gtaatattac gctcaaaaca ggacaattta atttgttagt tttaatagca ttagatgttt
    23341 ttttattctt tcttttagtt gtagtagcta ctttgaagag gagtagatga ttatataaaa
    23401 gcttattata attatgacaa tctgataatt gttaataaaa aagttgattc tttgctttat
    23461 ttataaaata ttatatgtat ttttgtattt taaacttaat aattattaat ttaaatttta
    23521 taactatgag gaaaattttt attttatttt tggctttaac ctttattttt ggaggtttag
    23581 ctaatgcaca acaattgaaa agcattgcta aggcaaaggc aagtgtaaat gcagttgcaa
    23641 atacaaatgc ggaaattggt gcatttaaaa tgaatggcga aaagccagca gaaaagccag
    23701 caacaggcaa gcaagtgaaa ttaaacagtt tgttaggttc tggagcaaag gctactacag
    23761 tttattctga agactttact ggaggtatgg gtggatggac agttgtgggc ttaggagacg
    23821 gaagttggtc tgttaaagat gaaagtaatg caggaggaac tgcacctgaa cttatgctag
    23881 aatgggtttc tggttctgat tttgatgggc agtcttattg catgagccca gttattaata
    23941 ctacaggcta cacaacatta gccttagaat ttaaacaatg gtttagaaac taccaaggct
    24001 cttctgtact tggtgtttat actactattg atgatggtgc aacttgggaa gaagtttggt
    24061 cagaaactgt atctgctaat tttgtagagg ttaaaaactt actgataact aatgctcatg
    24121 ttggaaatgc caattttaga attgcttttg gatttaatgg aaataccttt gatatatggg
    24181 aatgggcaat tgatgacatc ttattagttg aacctgatgc tcacgattta gccattgttg
    24241 ctattaacct taatacattt gtagaaagtg gttctactgt aacaccacaa gttactattc
    24301 gaaatatggg aataagcaat gaagctactt ggtctttaac tttaacagat ggagatgctt
    24361 atactagtac taaaagtgat attcttatta atataggaga caatttagta gttgatatgg
    24421 atgattggac accagctgat ggtcaatata cattaaccgc tacactaact ctcacagacg
    24481 atgcaaatac aagcaataac acattaacag ctactgttga tgtaattgag ttttctactg
    24541 ctttcgcatg gaatgcttat catgaaggag gcttaaacgc tactggagaa ggtcctgtta
    24601 acattgtttt acaaactggc atcatgactc aaatcgcaga ggatgacagt gactttatta
    24661 tggcagctga ttatgttgag gatgaaattt atggtattcg ttatgctgac ggcgtgcctc
    24721 gtcctttagt taaaattgac cctgttacag gagttgttac aaatattggt ggtggtgctg
    24781 tagatttagt tggttttact tatgatgtaa ctacagaaac tgcttatgtt atagatttta
    24841 atggtatgtt gggtactatt aaccttgaaa ctggtgaagt aacaaatatt ggcggagatt
    24901 acacaagtgt aataggctta gcatgtgata ctagtggtaa tttaattgct atttctttag
    24961 cagatcatat cgcaagtatt gataaaacta cgggaacatc tacaataatt ggaaatttag
    25021 gcgtagatat taattatgca caagatattt gctatgatag agataacaat attttatatg
    25081 gtgcattata tactgaccaa ggtgccatat atataataaa tacaacaaca ggtgcagcta
    25141 ctttggtttc tgaagtaata gatgaattaa ctggatttgc tattccttat actctagtcg
    25201 gtcctcaagt tgttttatta acgccagaag caaatgccgt aggtgttgca cttaatgctg
    25261 aagttagtgt aacttttgat gttgatctta cagctgttga tcttactgga attactatta
    25321 ctccagatcc aggtaatgtt tcagcttcag ttaataatgc agttttaaca attgctcatg
    25381 acaattttaa ttataataca gaatacacag ttttagttcc tgctggtgca gttagtgatg
    25441 gtactgatcc aactactcat cctattactt ggtcgttcaa aactgtatta gatcctacag
    25501 catgtaatga accaactggc attgtatttt ctgatatcca agctgaacaa gcaactgttg
    25561 cttggacaga aactggtgca ggtacaagct ggagagttaa atatggcgct actggttttg
    25621 acccagatac tgaaggtgaa gaagttgttg ttaatgaaca aacagctact ataacagaat
    25681 taacaggtaa tactacttat gaagtatatg tccaagcact ttgtggtaca gatgaaacaa
    25741 gtggttggag tggttcagct atattcgcta ctgcttgtgg tgctataact actttgccat
    25801 atcttgaaga gtttgaagga gcattcccta cagaatgttg gacatttgta aataatcatg
    25861 cagatcctac ttctaattgg catatcgaca attatcaaga tactggagac cctgcgctta
    25921 atatactctg gtctgatgag aacatgatgg atgaatgggc aataagtcct gtgtttgatt
    25981 taagtggaat tactggagaa attgcattaa catttgattt tttgatgagt tattattatc
    26041 aagtggatcc tactaatgga gctgacgtaa tggttaaagt tacaacagat aatggaacta
    26101 cttggactaa aatttggcaa gaagaagatt atggaacatt tgagaattgg acttggtaca
    26161 cagctaccct tccatttagt acatatgttg gagaatctaa tgttaaattt gctttccact
    26221 atttaggtaa tgatggtgct caagttatga ttgataatgt agaacttgat tacgcaacaa
    26281 gtgttaacga caatgtaact aatctagttt cagtttatcc taacccatca aacggcatag
    26341 ttaacgttca agttagcgaa acttctgtta ttacaatcgt tgatttagcc ggaagagttg
    26401 ttgctactta taatgctaat gcaaacgaaa cactaagctt cactcaagct gctggtgtat
    26461 atgttgtaaa agtagaaagc aacggtcaag tttctaatca taaattaatt attcaataaa
    26521 ctttaaagtt taatattata aaaagagagg tgagagcctc tctttttttt gtattaatac
    26581 caagttgcgt tctattataa aacattaaga taaggaaaaa gtgtcatag
//
LOCUS       DAOPNX010000024        26598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_2338_length_26598_cov_6.921976, whole genome shotgun sequence.
ACCESSION   DAOPNX010000024 DAOPNX010000000
VERSION     DAOPNX010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 26598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26598
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2338_length_26598_cov_6.921976"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="PKN53_04115"
     CDS             complement(<1..935)
                     /locus_tag="PKN53_04115"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOD88170.1"
                     /translation="MEINKELMQASIIGLRDAMGLKPEETLLVVTDEVKRDIGLALHE
                     AGKQLCKESILVEMKSREINGEEPPFAISEMMKMVDVVVIPTAKSLTHTDARRNAVKE
                     GVRVGTMPGISVEIMSRCLNADFDKIVELTEYVAKRLEGVKTVKVVTEAGTNLVMPIE
                     GRMIIPSTGVLREKGQSGNMPSGETYLAPIEGKSNGTLVIDGSMAGIGVIKTPIKVEI
                     RDGYAEEITGGEEAQRLIAMLDKNGREARAVAEFGIGTNYKAILSGQILEDEKVLGTI
                     HVAFGNNLTMGGSIAVNSHLDGLVKSPDVYFDDELV"
     gene            complement(983..3454)
                     /locus_tag="PKN53_04120"
     CDS             complement(983..3454)
                     /locus_tag="PKN53_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOD88171.1"
                     /translation="MKRFFLFMFFVFFAYFLQAQIISGKIVDENDNPLVGASVIIKNT
                     FLGTSSDLNGDFSIKSKFDNCEIVISYLGFESISQIVKLNGKNQNLGKLVLKPSAYMS
                     DEVIVKATRADDNSPIAFISMDKEDLQKRNTGQDLPYMLELTPSVVASSEAGTGVGNT
                     SFRVRGTDPTRINITVNGIPLNDSESQAVFWANMPDFAGSVNNLQLTRGVGSSTNGAA
                     AFGASMNIFTGSLSNKPYAEISSMMGSFNTFKENLKIGTGILKNGFSFDLRLSKLDSD
                     GYVENSYSDHYSMMMSAAWRGKTSFVRANIIRGNQKTGITWYGCPQEVLDTNRIYNPA
                     GEYFDEFGNRKMYKDNADNYIQTHYQIIFSKDFKRNLDLSAGIHYTRGDGYYEQYKMD
                     QKFSKYLLPNIVLQPDPIINGLDTLIPNAIVINMSDLVYRKQMANDFYGATSSLNYKN
                     EKLKLSFGAGWNKYDGDHFGNILWMKNLKDLPINYEWYRNYGEKTEFNVFSKINYEIA
                     KSLYLYGDLQYRNIRYFMQGEDYELDMNGNLIVLDQEHKFNFINPKAGLFYDISDKMN
                     VYASFAVANREPTRTNFKDAAGDTLKTPKAERLYDYELGYNYRSQRFSALVNLYYMDY
                     IDQLIPTGEKSNTGYDIMTNVAESYRAGIELVANYKPINKLSLEANLTLSRNKIKDFV
                     SWAATYDEDWNENYESFDLGERDIAYSPNIISSGIVSYKPFAGFNISWIAKYVGAQYF
                     DNTSSPDRKLDPYFVNNLQLDYSIETKFIKEINFSFIVNNIFNLKYSNNAYGGMWYEQ
                     GVEKTWAYYFPQAGINFMGGINLKF"
     regulatory      complement(3501..3591)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3653..4240)
                     /locus_tag="PKN53_04125"
     CDS             complement(3653..4240)
                     /locus_tag="PKN53_04125"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOD88172.1"
                     /translation="MKRIFDLLFSFLGLVILSPFLLVIAILIVIDSKGDIFYLQQRVG
                     KDNKDFNIFKFRTMRPNSDKQGLLTVGAKDSRITKIGVFLRKYKIDELPQLINVLIGN
                     MSFVGPRPEVRKYVDLYNSEQKKVLTVKPGITDYASIEYSNENELLARSENPEKTYIE
                     EIMPAKLELNLKYIKEASLLTDIKIIFKTFAKIVS"
     gene            complement(4240..>5247)
                     /locus_tag="PKN53_04130"
     CDS             complement(4240..>5247)
                     /locus_tag="PKN53_04130"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOD88173.1"
                     /translation="DEVIVPAYTYCASANVVVHCGAVPVLVDIDEKTFNLNISQLKNL
                     VNKRTKVIMPVDIAGLPCNYDEINNFVNSPAVKSLFKPKNEIQKTLGRILILSDSAHS
                     FGAEYKGHKTGSLCDISVFSFHAVKNLTTAEGGAVTLNLPKPFDNEELYKFLCVLSLH
                     GQNKDALAKTKGNWRYDVITAGFKANMTDIQASLGLVELERYESETLPRRKQIFDFYT
                     ESFKNNKNFTVPFYEDENYKSSYHVYLLRVNNVDEAKRDAIIQEIYENGVSVNVHFQP
                     LPLLSFYKGLKYKMKDYPQAYENYKCEISLPVYYDLSDEDAAKVVEVVKTAVAKIIEN
                     K"
     assembly_gap    5248..5347
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5375..6607)
                     /locus_tag="PKN53_04135"
     CDS             complement(5375..6607)
                     /locus_tag="PKN53_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOD88174.1"
                     /translation="MVLNYIWIAFFVIGFIVALVKLIFFGDVSVFPEIMNGTFDNAKL
                     GFELSLGLTGVMTLWLGIMKIGEKGGAINYLSRIISPLFNKLFPDIPKNHPVHGSMVM
                     NIAANMLGLDNAATPLGLKAMKELQEINPNKEKASNPMIMFLVLNTSGLTIIPISIMA
                     YRALMGAANPSDIFLPILIATFVSTLVGIITVSIFQKINLFCKEILIFLGGITLLMGG
                     LVYGFTLLAASQINVYSSLIANLILFSIIISFIVLGIRKKINIYDAFIEGAKDGFNVA
                     VKIIPFLIAILVAVGVFRASGALDFVMQGLKYLFASAGAGVEWIDALPVALMKPLSGS
                     GARGLMIDTMTMFGADSFAGRLACTFQGATDTTFYILAVYFGSVGIKNTRYAVSAGLI
                     SDLAGVIAAIFVAYLFFG"
     gene            complement(6739..7620)
                     /locus_tag="PKN53_04140"
     CDS             complement(6739..7620)
                     /locus_tag="PKN53_04140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOD88175.1"
                     /translation="MIKPYFKSSDKNFYLLHGNTMELLPQFEHKFDMVFADPPYFLSN
                     NGLSIQNGQIVSVNKGKWDKSEGFEFINDFNRKWLSLVRDKMKEDATIWISGTMHNIF
                     SVGQILTELGFKILNIITWEKTNPPPNFSCRYFTYSTEQIIWARKNEKVPHYFNYDLM
                     KQLNGNKQMKDVWTLPAIAPWEKSCGKHPTQKPLSVLTRLILASTKPNAWILDPFAGS
                     STTGIAANLLNRRYLGIDQEEDFLLISKNRKLEIENKKLAAKYKEKISGFNTKNELEL
                     FLFKEPKTEYGTELNFD"
     gene            complement(7620..8459)
                     /locus_tag="PKN53_04145"
     CDS             complement(7620..8459)
                     /locus_tag="PKN53_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HOD88176.1"
                     /translation="MSKQFEKFLSQLSETNATLDYFTDFDKVKENVNKISIKLTQLNY
                     LIGKKDLKKAVRELFDENPKVFEVLDILIAVRKNKTAKTFNNHGKVVILDDYFTSPEL
                     IVEYLEETGLYEIFKNKDITNLVDYVFGVEVGLDTNARKNRGGDNMSKAVSRIFNIAG
                     ISYEKEILSTSFSEIVSLGADVKQFDFVIKTKKKTYLIETNFYNSGGSKLNEIARSYS
                     DLAPKINQYDNYEFVWITDGQGWLTAKNKLEEAFNTIPKTYNLKTLKNFITLLKSEGV
                     IKL"
     gene            complement(8443..9354)
                     /locus_tag="PKN53_04150"
     CDS             complement(8443..9354)
                     /locus_tag="PKN53_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOD88177.1"
                     /translation="MAKPFLKWAGGKTQLLNDIELILPKNLVKTNFTYIEPFVGSGAV
                     LFWMLNNFPNIKKAVINDINTDLINTYKIIADKPNELISILEILQNEYHSLDEKADKK
                     KEYYYQKRELYNTRKSTNTEQAAFFIFLNRTCFNGLYRVNRKNEFNVPIGSYKKPMIC
                     DRENILAINKSLQKVEILSGDFEQTLKYANPNSLFYFDPPYKPLSNTSSFNSYTKDEF
                     DDLEQIRLRDFCNKLDVLGHIWILSNSDVKGKNPKDNFFDDLYSNFHIYRVEAKRTIN
                     ANPNKRGKLTELLITNQVDIKEYVQAI"
     gene            complement(9479..10717)
                     /locus_tag="PKN53_04155"
     CDS             complement(9479..10717)
                     /locus_tag="PKN53_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007481134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOD88178.1"
                     /translation="MKVALLTIGDEILIGQIVDTNSAWISKKLNEIGVKVEKILSIGD
                     TAEEIKWAVNFLMSENDIVVVTGGLGPTKDDITKTVLCEIFDTKLVFNQQALDNINEL
                     LGKRNVPINKNNFNQAMVPEAATIFNNKRGTAPAMLFEKNNSYLISMPGVPCEMQYIT
                     ETYLVPFVKEKFRINNIFHKTLLCSGIAESVLAEMLEDLEDSMPNYMKLAYLPALGFI
                     RLRLSIYDYKPEYQGAVDKVLEEIRKLIGNKILAEEDLKPEIIIGNILKSQNKTICTA
                     ESCTGGRIASLLTSVPGSSQYFYGSVVSYDNSVKRNLLGVSSEDLESYGAVSQQVVEQ
                     MAKGALELLKTDYAVSVSGIAGPDGGTSEKPVGTVWIAVASKEKILSKKVQFFNDREI
                     NIRRATNTALAMMIHFIKGE"
     gene            complement(10759..12498)
                     /locus_tag="PKN53_04160"
     CDS             complement(10759..12498)
                     /locus_tag="PKN53_04160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HOD88179.1"
                     /translation="MTNLRHSVVTKHDIQLVVKLKGIFGKFISSILYSVLGLKSLNKM
                     YNHCFDVDGEKFVENCLKYKNNKIKVYPQDLSYIPETGACVLLFNHPFGALEALMIYK
                     VLREKRKDVKFVANFLLSRIEPLKDAFVQVNPFETKKEVFSNLAGAKAMHKVIEDGGL
                     LCIFAAGEVSTKYKNSKYVEDKAWAQNIMRFIKSTNVPVISGFVDGQNSRCFHFLGKI
                     NPILRTASLPRQLLNKKNLEVCLRFTPPLSPKNIEQIENNKLLADFLKSRTYLLKYEE
                     VEEYNSDQKKYAEIIEAQDKDLLKAEIEKIKQTDLLFTTENYLVFFSEKKDIPVMFTE
                     LSRCREIAFRAIGEGTGDATDEDKYDDYYNHLFIWDEVENALVGAYRIGIGKPILEKF
                     GKKGFYLNTLFKFQDGFEEYLEKSMEMGRSFIVPEYQRRTLPLFLLWRGIYFVTQKYP
                     EYKYLIGPASISSIYSENAKILMIEFLKRYHGWQELSKFVEPRTKYEYKLNHHHEKVF
                     EVYADDLTLIDRLIRDIDINHYAMPVLIKKYLSLGGKILDFNVDPEFNFAIDGLVVLD
                     IGVVSQEVINSYK"
     gene            complement(12662..13573)
                     /locus_tag="PKN53_04165"
     CDS             complement(12662..13573)
                     /locus_tag="PKN53_04165"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOD88180.1"
                     /translation="MQFEIKILGSSSALPSSTRMPSAQMVLYNKTPYLIDCAEGTQMQ
                     MRKFSIPFGRLKNIFISHLHGDHFYGIFGLISSLNLLGRKQDLHIYAPAQLEEIISSL
                     HKINGDELKYKLIFHSLNSNGKNLIFENKNLEVYSFPLKHSKQVWGFLFQEKEKPRNI
                     KKEAIEKHNLSISQIVKLKNGEDIIDENGILIKNQELTYAASIPRSYAYCTDTIPVRN
                     LDKYIYNVDLLYHEATFGNDLADVAKQTLHSTAREAGEIAKLVNAKKLVIGHFSVRYK
                     NMNHLLKEAQEVFENTIIAEDGLSIEL"
     gene            complement(13583..15835)
                     /locus_tag="PKN53_04170"
     CDS             complement(13583..15835)
                     /locus_tag="PKN53_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOD88181.1"
                     /translation="MKYLLFFSVLFCLSISLFSQNYYWVFYKDKANTSFDPYKYFDTK
                     AIERRENLGISLFDLSDFPLNETYVATISDYCDEYIGESRWLNASAIYTDEIRIDIIS
                     KFEFVLDIQLIDNDLALCESKGVFTEEHDSAKVYLPQIKRMEGDKFIEKGINGKGKRI
                     AVFDGGFPNVDTHQAFKHLRDNNQIVKTWNFPRKIENVYGWNSHGTSVLACIAGVDED
                     GKNIGLATGAEFLLARTEIGPEPAREEVWWAMALEWADKNGADIVNSSLGYGKERRKL
                     KQMDGKTSVVAKAANTALEKGILVVNAAGNEGTDKSWQKIITPADSESVLAVGGVDYE
                     GKAHIGFSSYGPSYDGRLKPNVSAYGSVYTATTKSYGQLYGTSFASPLVAGFVACAWQ
                     TKPDLSAKEMKLEIEKSADKYPYFDYANGYGIPMASYFLNESKTFAKSFEIEEHENIV
                     EIKLLNSEEEKDLGYVFYHIRNSEGKLVFYEQVKVFSLRNSKISLPKSAFPDENSVLA
                     VWYQGYNEEYKLKNYKEYVDNDFNPSYVGTRSRRSQKPGQEKYSKFGANNCFYIEPFI
                     RKGYVFGDKSDNYDMSSAGNFTFGIRYKQKIVNAFSLGLNFSYSKSKVHLNDFSDFVN
                     NNRVSLISYKENFELKAFTGEFYGRIRFVAAGQSGLGLHLDVGAYADCVFSTNFRLKY
                     KTIDTKVKSLEKYSINDNLQYGLAAKFGYGMFSVYYKYALSELSVKNQYVLPNMEAGV
                     EISIPLGIIF"
     gene            complement(15853..17097)
                     /locus_tag="PKN53_04175"
     CDS             complement(15853..17097)
                     /locus_tag="PKN53_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOD88182.1"
                     /translation="MKKIVCYSFILTFLLFSCKTKKEMKNSSGMSTTGAKIEANQNQK
                     LTNFSFKLYSNLIKDEPRKNYSFSPVSLNLAMGMAYAGAEGKTADDISNVMGFYRDKQ
                     NFLLSISEYMDLLDKIAQDSLVEFRLANKIYLEQTYNILSSYQAKIEKYFDGAFQLAD
                     FINNAQKEAKNINSWVEKLTNNRIKNLIPDGTLSPSTAMVLVNAIYINSDWKHQFKKE
                     RTQKKDFFLSKKEKLETDFMIQKIKKIKYAEINSYKAIEMEYVSNDLSLLFILPVESN
                     IDNISDVVLNGEQYKKICNSLRYEEVHIEIPKFKIESSFNLSQMLKKMGMSSAFSNAD
                     FSGISQNNNLQISQVLQKVFFEVDEKGTEAAAATAMIVQTTAFIQNPNPTYEFIANRP
                     FFYVLKENKYNTPLFIGQFVKP"
     gene            17232..18002
                     /locus_tag="PKN53_04180"
     CDS             17232..18002
                     /locus_tag="PKN53_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOD88183.1"
                     /translation="MKKLIQDLKVVSNIKLNANYYLLKLFSEEDLPLILPAQFVEIKI
                     PNSPSTFLRRPISIHDVNYEKNTIKLLINIAGKGTESLSKIQANDYLNIVYPLGNSFT
                     MPTCDKVLLVGGGIGVAPLLYTAKYFLSFGIESDFLLGYRSKSNIVLHDKFSKYGKVF
                     YTTDDGSFGEEGHVLNHSIFTGSVFNYKKIYACGPKIMLKNLSIWANERNIDCEVSLE
                     NLMACGVGACLCCVQNTVDGHKCVCTEGPVFNSKEIIW"
     gene            17996..18907
                     /locus_tag="PKN53_04185"
     CDS             17996..18907
                     /locus_tag="PKN53_04185"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOD88184.1"
                     /translation="MVNLEINFAGLKFKNPILTASGTFGYGTEFADFIDLNLLGGFIV
                     KGTTLNYREGNPYPRMAETASGMLNAVGLQNKGVKYFCENIYPEIKDLKTNIFVNVSG
                     STIEDYVATAEMINNLENIPGIELNISCPNVKEGGMAFGTSCTSAAQVVKEVRKVYKK
                     HLMVKLSPNVTDIVSIAQAVESEGADSISLINTFLGMAIDAKTRKPKLSTITGGLSGP
                     AIKPIALRMVWQVSKAVKIPICGLGGIMNAEDVIEFMLAGASCVQIGTANFIDPDITI
                     KIINDLQVFCENQKIKNIDEIIGGLRL"
     gene            complement(18926..19366)
                     /locus_tag="PKN53_04190"
     CDS             complement(18926..19366)
                     /locus_tag="PKN53_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88185.1"
                     /translation="MKKIDVLISFVVLLMFFLSCEKRTIPEGYYSFTFKNYGSNLTAR
                     LSHSQVVESTRDYIVIKPYKETLYKDGKNITGILTHHGDIRGGEGLIYYGPFHITGVC
                     DKKKGKHYISGTFASEILVPNVQEEKLDTTYTSGTFEFKQYFGI"
     gene            complement(19682..20038)
                     /locus_tag="PKN53_04195"
     CDS             complement(19682..20038)
                     /locus_tag="PKN53_04195"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOD88186.1"
                     /translation="MKTTFLIIAIAFLSITAYADNNKSSKNVKTNKTEAVEKANFVNV
                     SGIITDKKSNESLAGASIYIDGTKYYSDLDGNFTIQNIKPGKHTVKVEFISYQSVEME
                     MDIRTNQEINIDLVQE"
     gene            complement(20154..20630)
                     /locus_tag="PKN53_04200"
     CDS             complement(20154..20630)
                     /locus_tag="PKN53_04200"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOD88187.1"
                     /translation="MRHKLILITVLFLVVFSLQAQKTPQIYQNEGVYYRDKKQNELYT
                     GDYREYYENKTLKLEMQIKNGLPEGTYIIYFENRKPKEIRSYKEGKLHGLWRNYDISG
                     QLISEAEYKNGQKHGTWRIWDELGMQRYEMNYYEGEKTGIWRMWDEKGTIVDEKQY"
     gene            21199..21471
                     /locus_tag="PKN53_04205"
     CDS             21199..21471
                     /locus_tag="PKN53_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOD88188.1"
                     /translation="MNKAQLIDAIAKKGNIKNAEAKNALELTLGAISEALKSGESITL
                     IGFGTFKIVDIAERTGRNPQTGEKIQIKAKKVVKFKPGNELSGHVK"
     gene            complement(21585..22019)
                     /locus_tag="PKN53_04210"
     CDS             complement(21585..22019)
                     /locus_tag="PKN53_04210"
                     /inference="COORDINATES: protein motif:HMM:NF039676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6169 family protein"
                     /protein_id="HOD88189.1"
                     /translation="MHNEYEINEFVNPKGFGYCFNATDKCKYTLLFNNLGGDKFIFEF
                     DKTESLDKNSINNKLIRDSICKVIYNFITANPERVIFIVYDNADYLDKARYRLFNIWF
                     KHYNKSNYFKKMNILTSKQEIVSIIFMSNNVKFIKEILYKDS"
     gene            complement(22019..23038)
                     /gene="gwsS"
                     /locus_tag="PKN53_04215"
     CDS             complement(22019..23038)
                     /gene="gwsS"
                     /locus_tag="PKN53_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="grasp-with-spasm system SPASM domain peptide
                     maturase"
                     /protein_id="HOD88190.1"
                     /translation="MNSESKYFKLFAECKLVKGNKRSAIYDLPRNSYKLIPNGLYDIL
                     STYKTNTIDDVKRDFEKTQHSIIDEYFKMLIENEFIFFCDEDELELFVDLEPAWDYPG
                     QVSNAILELPMQNDTLTLSLLSLEKLGCRFIQFRAFNTLNLNYIEAILTFLNNKDFDY
                     IEIISKWGNYDIDKLISILKNHQRLRLLYFYGAEKNKVLKDNKIERQKIVFSSEMKEK
                     HSDFHVNNKYYFKVNITLFCESLFFNNYYNRKVCITKEGEIKNCLSLNKTFGYVKNLD
                     SIDRIVQTNDFQLLWNINNDKIKICQDCEFRYMCVDNIPLKYENGEWIKTSPCFYNPY
                     NPDDL"
     gene            complement(23028..23228)
                     /locus_tag="PKN53_04220"
     CDS             complement(23028..23228)
                     /locus_tag="PKN53_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88191.1"
                     /translation="MESKSIKDLYDKDFDKLPTNVQFLKIEEKLKDDHLKIIKASNNT
                     SDYYPQNFYKIISPIINLKNEF"
     gene            complement(23218..24201)
                     /gene="gwsG"
                     /locus_tag="PKN53_04225"
     CDS             complement(23218..24201)
                     /gene="gwsG"
                     /locus_tag="PKN53_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="grasp-with-spasm system ATP-grasp peptide
                     maturase"
                     /protein_id="HOD88192.1"
                     /translation="MILIISEPDDATTDSVINWLKYYNVEYLRINNTNVFDFFKLTIN
                     ADGNIDFVCQHDDTVIQLSKITGYWYRRGKLSLNVNAVGKQKYLNLSEVTNRFLLNEF
                     DAITEFIYNELHNIKKSVGNIYENNTNKLINLKIANKCGMNIPRTWILTNKQEVQDII
                     KEYKILLTKPITQSGLYYENDEICFSGQSYLFTEEIINNFPDTFAPTLFQEYIDKAYE
                     LRVFYFKEEIYISAIFSQLDEMTKIDFRNYNFQKPNRTPPYKAPDSLKLAIIKFMDKT
                     NMKSGSIDILVGKDKRYYFLEVNPIGQFYQVSYPCNFYLEKRIAKYFKNGK"
     gene            complement(24223..24855)
                     /locus_tag="PKN53_04230"
     CDS             complement(24223..24855)
                     /locus_tag="PKN53_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88193.1"
                     /translation="METIQDFDSLYSIYKASINLNLANQIDSLFEIDQKYTQKVNDGF
                     ILFRHTIFGLKWIINNKKQFTIINNIIKEYGFPGEKLIGLPKTYEDSVSTVRYISFWE
                     PYIREWQAYFMLLHYFSTKRNVSVDFKNTLFQNVINGNMTPFQYANICRYIYRHSKNT
                     DYESYYGNDTITNRKRLEVGLNTVEQEKRNELIQRERRKNKKANDEIMLE"
     gene            complement(25295..25663)
                     /locus_tag="PKN53_04235"
     CDS             complement(25295..25663)
                     /locus_tag="PKN53_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88194.1"
                     /translation="MKKKDLQSLNEELFNSEEILSNELRELVEVVGGAATPATSKNED
                     CSSCTYDCTGASNDTSKYRTDSSAGPEGGTDTIRKDDGCNEQAAQAVLKAIDDYNKNS
                     NGVVSGDLMEYEGPCCKEYY"
     gene            25774..26232
                     /locus_tag="PKN53_04240"
     CDS             25774..26232
                     /locus_tag="PKN53_04240"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HOD88195.1"
                     /translation="MKRFKHIKQNDHNDCGPTCLRIVAYWHGKNYDIDFLKKNCNINN
                     DGVNLLDIVKAAELIGFKTLCVKIDTKKLDKVLEIGPCIIHWQKKHFVVLYKQKKSFW
                     KRSKIYYISDPARGRLTFNEEIFSKSWKGNSAKGYCLLLEPKEDFYNLKF"
BASE COUNT         8877 a         4381 c         3465 g         9775 t
ORIGIN      
        1 acaagttcat cgtcgaaata aacgtctgga gattttacaa gtccgtctaa atggctatta
       61 acagcaatac ttccacccat tgtaaggttg tttccaaatg ctacatgaat agttcctaaa
      121 actttttcat cttctaaaat ttgtccagaa agtattgctt tataatttgt gcctattcca
      181 aattcggcta cagctctggc ttctcttcca tttttatcga gcattgcaat tagtctttga
      241 gcttcttcgc ctccagtaat ttcttcggca tagccatcac gaatttctac ttttataggc
      301 gtttttataa ctccaattcc agccatagag ccgtcaatta ccaaagttcc gttagattta
      361 ccttctattg gtgctaaata tgtttctccc gaaggcatat ttccactttg tcctttttcg
      421 cgtaaaactc ctgtgcttgg aattatcatt ctgccttcta ttggcataac taaatttgtt
      481 cctgcttcgg taacaacttt tactgtttta acgccttcta atcgttttgc tacgtattct
      541 gttaattcta caattttgtc aaaatcagca tttaaacaac gcgacataat ttccacagaa
      601 attcctggca tagtaccaac tctaacgcct tctttaactg cattgcgtct tgcatctgta
      661 tgtgtcaagg attttgctgt aggaataaca accacatcaa ccattttcat catttccgaa
      721 attgcaaatg gaggttcttc gccattaatc tcacgcgatt tcatttcaac caaaatactt
      781 tctttacata attgtttgcc agcttcgtgc aaagctaagc caatgtctcg ttttacttcg
      841 tctgttacaa caagtaaagt ttcttcgggc tttaacccca tagcatcacg caaacctata
      901 attgatgcct gcattaattc tttatttatt tccatcttaa atatttttac gaaagttcaa
      961 atttataaaa aattaacgaa ttttaaaact ttaaatttat tcctcccata aaattaattc
     1021 ctgcctgtgg aaaataatat gcccaagtct tctctactcc ttgttcgtac cacattccac
     1081 cataagcatt atttgaatat tttaaattga aaatattgtt tacaataaaa ctaaaattaa
     1141 tttcttttat aaattttgtt tctatgctat aatccaattg caaattattt acaaaatatg
     1201 gatctagctt tctgtcaggc gaagaagtat tgtcaaaata ttgagctcct acatattttg
     1261 caatccaact tatattaaac ccagcaaatg gtttatacga aacaattccc gatgaaataa
     1321 tatttggcga ataagcaata tctcgttctc ccaaatcaaa tgattcatag ttttcgttcc
     1381 aatcttcgtc ataagttgct gcccaagaaa caaaatcttt aattttattt ctactcaaag
     1441 ttaaatttgc ctccaagcta agtttattta ttggcttata gtttgcaaca agctcaatac
     1501 cagccctata cgactcagca acatttgtca taatatcata tcctgtattt gatttttcgc
     1561 ctgttggaat aagttggtcg atataatcca tataatacaa gtttacgagt gcagaaaaac
     1621 gctgagaacg ataattatag cctaattcat aatcgtaaag tctttcggct tttggtgttt
     1681 ttaaagtgtc gccagcagca tctttaaaat tagttcttgt aggctctcta tttgctacag
     1741 caaaagaagc gtaaacattc attttgtcag aaatatcata aaataatcct gctttaggat
     1801 ttataaaatt aaatttatgc tcttggtcta aaactattaa attaccattc atatctagct
     1861 cataatcttc accttgcata aaatatctta tatttctata ctgcaaatct ccatacaaat
     1921 aaagtgattt tgcaatttca taatttattt ttgaaaaaac attaaactca gttttttctc
     1981 cataatttct ataccattca taattaatag gtaaatcctt caagtttttc atccacaaaa
     2041 tatttccaaa atgatcgcca tcatatttat tccagcctgc tccaaagctt aattttagtt
     2101 tttcattttt ataatttaaa gaacttgtag ctccataaaa atcatttgcc atttgttttc
     2161 tataaactaa atcagacatg tttattacaa tagcatttgg aattaaagta tccaagccat
     2221 tgataattgg atctggctgc aacacaatgt ttggtaataa atattttgaa aatttttgat
     2281 ccattttata ttgttcgtaa taaccatcgc cacgagtata atgtatgcca gcactcaagt
     2341 ctaagtttcg tttaaaatct tttgaaaata tgatttgata atgagtttga atatagttgt
     2401 cggcattatc tttatacatt tttctatttc caaattcatc aaaatattcg ccagcaggat
     2461 tataaattct atttgtatct aaaacttctt gtggacagcc gtaccaagtt attcctgttt
     2521 tttgatttcc tcgaataata tttgctctta caaaacttgt ttttcctctc caagctgcac
     2581 tcatcatcat agaatagtga tcagaatatg aattttcaac atatccatcg ctatcgagtt
     2641 ttgaaagtcg taaatcaaaa gaaaatccgt ttttcaaaat tcctgttcca atctttagat
     2701 tttctttaaa agtgttgaaa cttcccatca tgcttgaaat ttcggcataa ggcttattgc
     2761 ttaacgagcc tgtaaaaata ttcatacttg caccaaaagc ggctgctcca tttgttgaag
     2821 agccaacacc acgtgtcagt tgcaaattat ttacagagcc tgcgaaatcg ggcatatttg
     2881 cccaaaaaac agcttgggac tctgaatcgt tgagaggaat tccgtttaca gtaatattta
     2941 tacgagttgg gtctgttcca cgcactctaa aacttgtatt tccaactcct gtacctgcct
     3001 cgctacttgc aacaacagaa ggtgttaact ccaacatata aggcaaatct tgtcctgtat
     3061 tgcgtttttg caaatcttcc ttatccattg atataaaggc aattggagaa ttatcatcag
     3121 cccttgttgc tttcacaata acctcatcgc tcatataagc tgatggtttt agtactaatt
     3181 ttccaagatt ttgatttttg ccattcaatt tcacaatttg cgaaatagat tcaaaaccaa
     3241 gataagaaat tacaatttca caattgtcaa atttagattt gatagaaaaa tcaccattga
     3301 gatcggaact tgttcccaaa aaagtgtttt taataatcac acttgctcct acaagaggat
     3361 tatcattttc atcaacaatt tttccgctaa taatttgagc ttgcaagaaa taagcgaaaa
     3421 acacaaagaa catgaataaa aaaaatcgtt tcataataat taaatattaa gttaataaaa
     3481 atttaattgg ggaaactaaa cggttgtaat caattcctcc ctacggcggc attacccgca
     3541 tcaggttcaa agggtatgat ctcagcctgt ttggcacccc tgtcataatg acgatacaaa
     3601 gttaatattt attttttagg aataaaaatg ttttggaaca agttttaaaa atctatgaaa
     3661 cgattttcgc aaaagtttta aaaattattt taatgtctgt tagcaaactt gcttctttga
     3721 tatattttaa atttagttca agttttgcag gcattatttc ttcaatataa gttttttcgg
     3781 gattttcgga tcttgctaac aattcgtttt cgttactgta ttctattgag gcataatcag
     3841 ttatacctgg ttttactgtc aaaacttttt tttgttccga attgtataaa tcaacatatt
     3901 ttctgacttc gggtcgtggt ccaacaaatg acatgttacc tattaataca ttaataagtt
     3961 gaggcaattc gtcaatttta tattttctta aaaacactcc tattttagta attctgctat
     4021 ctttcgcacc aactgtaagc aaaccttgct tatcggaatt tggtctcatt gtacgaaatt
     4081 taaaaatatt aaaatcttta ttatccttgc ctactctctg ttgtaaataa aatatatcac
     4141 ctttagagtc aattacaatt aaaattgcaa tcactaacaa aaagggagat agaattacta
     4201 atcccaaaaa tgaaaacagt aaatcaaaaa ttcttttcat tatttatttt caataatttt
     4261 tgcaacagca gttttcacaa cttcaacaac tttagctgca tcctcatcgc tcaaatcgta
     4321 ataaacaggc aaagaaattt cgcatttata attttcgtaa gcttgaggat aatctttcat
     4381 tttatatttt aaacctttat aaaaagaaag caaaggcaaa ggttgaaaat gaacatttac
     4441 cgaaactcca ttttcataaa tttcttgaat aatagcatct cttttagcct catcaacatt
     4501 attaactcgt agcaaataaa cgtgataaga agatttgtag ttttcatctt cataaaatgg
     4561 aactgtaaag tttttattat ttttaaaact ttcagtataa aaatcgaaaa tttgttttcg
     4621 tcttggtaaa gtttcgcttt catatctttc aagctctact aagccaagag aagcctgaat
     4681 atctgtcata tttgccttaa agccagcagt aatcacatca tatctccaat ttccttttgt
     4741 ttttgcaaga gcatctttat tttgtccgtg caagctcaaa acacaaagaa atttatataa
     4801 ttcttcgtta tcgaaaggtt ttggtaaatt taaagttaca gctccacctt cggcagttgt
     4861 aagattttta accgcatgaa aagaaaaaac agaaatatcg cacaaagaac ctgttttatg
     4921 tcctttatat tctgctccaa aagaatgtgc agaatcagac aatattaaaa ttcttcctaa
     4981 agttttttga atttcatttt taggcttaaa aagtgattta acagctggtg agtttacaaa
     5041 attattaatt tcatcataat tgcatggtaa accagcaata tcgacaggca taataacttt
     5101 tgttcgttta tttacaagat ttttaagttg tgagatattc agattgaaag ttttttcgtc
     5161 aatatccact aaaacaggaa ctgctccaca atgcactaca acatttgccg aagcacaata
     5221 agtatatgca ggcacaatta cttcatcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnata ttacaaaagt aattataaac aacatcaacc aaagaaaagg taagccacaa
     5401 atatagcagc aattacccct gccaaatcag aaattaaacc ggcagagacg gcatatcttg
     5461 tatttttaat tcctaccgag ccaaagtaaa ctgctaaaat atagaaagtt gtatctgttg
     5521 ccccttgaaa agtacaagcc aaacgtccag cgaaggaatc tgcgccaaac attgtcattg
     5581 tgtcaatcat taatcctctt gctccacttc cactcaaggg tttcattaaa gctactggca
     5641 aagcatcaat ccactccact cctgctccag cagaagcaaa taaatatttt aatccttgca
     5701 tcacaaaatc taatgctcct gaagctctaa aaacaccaac agcaaccaaa atagcaatta
     5761 aaaaaggaat tatttttacc gcaacattaa atccgtcttt tgctccttcg ataaaggcat
     5821 cgtaaatatt tatttttttt cttatcccca aaactataaa actaataatt attgaaaata
     5881 aaataagatt tgcaataagt gaggaataaa catttatttg tgatgcagcg agcaaagtaa
     5941 aaccataaac taagccaccc attaaaagtg taattccacc aagaaaaatc aatatttctt
     6001 tacagaataa atttattttt tgaaaaattg acactgtaat aattcctact aaagttgata
     6061 cgaatgtagc aattaatatt ggcaaaaata tatctgaagg atttgctgct cccattaaag
     6121 ctctataagc cattatgcta atagggatta ttgttagtcc cgaagtgttt aaaaccaaaa
     6181 acattatcat cgggtttgag gctttttctt tatttggatt tatttcctgc aactctttca
     6241 tagcttttaa gccaagtggc gtagctgcat tatccaatcc aagcatatta gcagcaatat
     6301 tcataaccat agaaccatgc actgggtggt ttttgggaat atctgggaaa agtttattaa
     6361 acaatggaga aataattctt gataaataat ttatagctcc tcctttttcg ccaattttca
     6421 taattccaag ccacaaagtc ataacacctg tcaatcccaa cgaaagctca aaaccaagct
     6481 tagcattatc aaaagtgcca ttcataatct caggaaaaac acttacatct ccaaaaaaaa
     6541 ttagttttac aagtgcaact ataaaaccaa ttacaaaaaa tgctatccaa atataattta
     6601 ataccaaaac aaaaaacttt ttttgtaaag atacaagcta atagaaaaga aatttaaaat
     6661 atgaaaactt tgtattaaca tagttttgaa caaaaattga tgggtaccag taaaaaatat
     6721 ttataaaact aactcatttt aatcaaaatt aagttcagtt ccatattctg ttttcggttc
     6781 tttaaataaa aataattcta attcattttt agtattgaag ccactaattt tctccttata
     6841 tttagcagca agttttttgt tttctatctc aagttttcga tttttactaa ttagtaaaaa
     6901 atcctcttct tggtcaatgc ctaaatacct tctatttaat aaattagcgg caattcctgt
     6961 tgttgaactt ccagcaaaag gatccaaaat ccaagcattt ggttttgtag aagccaaaat
     7021 tagacgagtt aaaactgaaa gaggtttttg tgttggatgt tttccacatg atttttccca
     7081 aggagcaata gctggtaaag tccaaacatc tttcatttgc ttattcccat tcaactgctt
     7141 cattaaatca taattaaaat aatgaggtac tttttcgttc tttcttgccc aaataatttg
     7201 ttctgtcgaa taagtaaaat atctacaaga aaaatttggt ggtggattag ttttttccca
     7261 agtaataata ttcagtattt taaagccaag ttctgtaaga atttgaccaa cagaaaaaat
     7321 attatgcata gttccactaa tccaaatagt ggcatcttct ttcattttgt cacgcactaa
     7381 cgacaaccac ttgcgattaa aatcattaat aaactcaaaa ccttctgact tgtcccattt
     7441 gcctttattc acacttacaa tttgcccatt ttgaatagac aatccattat ttgacaagaa
     7501 ataaggcgga tcagcaaaaa ccatatcaaa cttatgctca aattgtggca aaagctccat
     7561 agtatttcca tgaagaagat agaaattttt gtctgaagat ttaaaataag gttttatcat
     7621 tatagtttaa ttacgccttc acttttcagt aaagttataa aattctttaa agttttcaaa
     7681 ttgtatgttt ttggaattgt attgaaagct tcttctaact tatttttagc agtaagccaa
     7741 ccctgtccat ctgttatcca aacaaattca taattatcat attggttgat ttttggtgca
     7801 agatcagagt aagaacgagc aatttcattt agtttagagc caccgctgtt gtaaaaatta
     7861 gtttcaatca aataagtttt ctttttagtt tttattacaa aatcaaattg cttaacatct
     7921 gcacctaaac taacaatttc ggaaaaagaa gtactaagta tttctttttc ataagaaatc
     7981 cctgctatgt taaaaattcg agaaactgct tttgacatat tatcgccacc tctgttctta
     8041 cgagcattag tatctaatcc aacctcaact ccaaagacat aatcaactaa atttgtaata
     8101 tctttatttt taaaaatttc atacaaacct gtctcttcca aatattctac aattaattca
     8161 ggcgaagtaa aatagtcatc taagattact actttaccat ggttattaaa tgttttcgct
     8221 gttttatttt ttcggacagc aattaatata tctaatactt cgaaaacttt tggattttca
     8281 tcgaataact ctctaacagc tttttttaaa tccttttttc caatcaaata attcaactga
     8341 gttaatttaa ttgaaatttt atttacattt tcttttacct tatcaaaatc ggtaaagtaa
     8401 tcaagtgttg catttgtttc agaaagttga gataaaaatt tttcaaattg cttggacata
     8461 ttcttttata tcgacttgat tggttattaa taattctgtt aatttccccc ttttgttagg
     8521 gtttgcatta atagttcttt ttgcttcaac tctataaata tggaaatttg aatataaatc
     8581 gtcaaagaaa ttatcttttg gatttttccc tttcacatct gagttgctta aaatccatat
     8641 atgacccaag acatctaatt tattacaaaa atctctaagt cgaatttgtt ctaaatcatc
     8701 aaactcatct tttgtatatg agttaaaact tgatgtgttg ctcaaaggtt tatatggtgg
     8761 atcaaaataa aataaagagt ttggattggc atattttaat gtttgctcaa agtctccgct
     8821 taaaatttca actttttgaa gagacttgtt tattgccaaa atattttctc tatcgcaaat
     8881 catcggcttt ttataacttc caataggaac gttaaattca ttttttctat ttactctata
     8941 caaaccatta aaacatgtgc gatttaaaaa aataaaaaaa gctgcttgtt ctgtatttgt
     9001 acttttacgc gtattataca attctctttt ttgataataa tactcttttt tcttatcggc
     9061 tttttcatct aagctatgat attcattttg taaaatttca agaattgaaa taagttcgtt
     9121 tggtttgtct gctattattt tatatgtatt gattaagtct gtattaatgt catttatcac
     9181 agcctttttt atatttggaa aattatttaa catccaaaat aaaactgctc cacttccaac
     9241 aaaaggttct atataagtaa aatttgtttt tactaaattt tttggcaaaa tcaattcaat
     9301 atcattgaga agttgagttt ttccacctgc ccattttaaa aaaggttttg ccattttatt
     9361 tatattcatt ttttcttttg ttatttatgc gtcaaattta caaatttaat tgggataata
     9421 ctattaaaaa ttactggttt tacaatttat ttattaaaaa tttaggtatt acaaagcatt
     9481 actctccctt aataaaatgt atcatcattg ccaaagcggt atttgtggct cgtcttatat
     9541 ttatttccct atcattgaaa aattgaactt ttttggacag gattttttct tttgaagcaa
     9601 ctgcaatcca gactgttcct actggttttt cgcttgttcc gccgtctggt ccagcaatac
     9661 cacttaccga aacggcataa tcagttttca ataattctaa agctccttta gccatttgct
     9721 ctacaacttg ctgagaaaca gctccataac tttctaaatc ttcagaactt actcctaata
     9781 aatttcgctt tacagaatta tcataagaaa caactgaacc ataaaaatat tgagaacttc
     9841 ctggtacaga agttaataaa gaagctattc gtccacctgt acacgattcg gctgtacata
     9901 tagttttatt ttgacttttc agaatattgc cgataattat ttcgggtttt aaatcctctt
     9961 ctgccaaaat tttatttcca attaattttc taatttcctc caacactttg tccacagccc
    10021 cctgatattc aggcttataa tcataaatac taagtcttaa tctaataaaa cctaaagctg
    10081 gtaaataagc caatttcata taatttggca ttgagtcttc taaatcttca agcatttcgg
    10141 caagcaccga ctctgcaata cctgaacata aaagcgtttt gtgaaaaata ttatttattc
    10201 taaatttttc ttttacaaat ggaactaaat aagtttcggt aatatattgc atttcgcaag
    10261 gcacacctgg cattgaaatt agatatgaat tatttttctc aaataacatt gctggtgcgg
    10321 ttcctctttt attattaaaa attgttgcag cttctggcac catagcttga ttgaagttgt
    10381 ttttatttat tggcacatta cgctttccca acaattcatt tatattgtct aaagcttgtt
    10441 gattaaaaac caattttgta tcaaaaattt cgcataaaac agtttttgta atatcatctt
    10501 ttgttggacc caaaccgcct gtaacaacaa ctatatcatt ttcggacatt aagaagttta
    10561 cagcccattt tatctcttcg gcagtatcac caatagataa aattttctcg accttaactc
    10621 ctatttcatt aagttttttg ctaatccagg ctgaatttgt atcaacaatc tgtcctatta
    10681 aaatttcatc gccaatagtt aaaagtgcaa ctttcataaa taattttcaa aacaaaaaaa
    10741 ctggaaaaaa taaaaatctt acttatacga atttataact tcttgagata ctaccccaat
    10801 atccaaaact accaatccat ctatagcaaa attaaattct gggtcaacat taaaatctaa
    10861 tatttttcca cctaaagaaa gatatttttt tattaacaca ggcattgcat aatgattgat
    10921 atctatatct ctaattagtc tatctattaa agtcaaatcg tctgcataaa cttcaaaaac
    10981 tttctcatga tgatgattta atttatattc atatttagtt cttggctcta caaatttact
    11041 caattcttgc caaccatgat atcttttcaa gaactcaatc attaaaattt tagcattttc
    11101 ggaatatata cttgaaatgc tggctggacc aattaaatat ttatattctg gatatttttg
    11161 tgtaacaaaa taaattcctc tccacaacaa aaacaatggt aatgtccttc tttgatattc
    11221 tggaactatg aaagaccttc ccatttccat tgatttttcg agatattctt caaatccatc
    11281 ttgaaattta aaaagcgtat tcaaataaaa accttttttg ccaaattttt ctaatatagg
    11341 tttgcctatt cctattcgat acgcaccaac taaagcattt tcaacttcgt cccaaataaa
    11401 taaatgatta taataatcat catatttatc ttcatctgtt gcatcacctg taccttctcc
    11461 tatcgctcta aaagcaattt ccctacatcg cgacaattca gtaaacatta caggaatatc
    11521 ttttttttct gagaaaaata ctaaataatt ttctgtagta aataataaat ctgtttgttt
    11581 aattttttca atctctgctt ttagcaagtc tttatcttga gcttcaataa tttcagcata
    11641 ttttttttgg tctgagttat attcttctac ttcttcgtat tttaataaat atgttcttga
    11701 ttttaagaaa tctgctaata atttattgtt ttctatttgt tctatatttt taggtgataa
    11761 aggtggtgta aatctcaaac aaacttctaa atttttctta tttaaaagtt ggcgaggcag
    11821 gcttgctgtt ctgagtatag gattgatttt tcctaaaaaa tgaaaacatc tactgttttg
    11881 accatcaaca aagcctgaaa tgactggcac attggtagat ttaataaatc tcattatatt
    11941 ttgtgcccaa gctttatctt caacgtattt tgaattttta tatttagtag atacttctcc
    12001 tgctgcaaat atgcacaaca aaccaccatc ttcaataact ttatgcatag ctttagcacc
    12061 tgctaaattt gaaaaaactt cttttttagt ttcaaaagga tttacttgca caaaagcatc
    12121 tttcaaaggt tctattcttg aaagtaaaaa atttgcaaca aactttacgt cttttctttt
    12181 ttcccttaaa accttataaa tcattaaagc ttctaaagca ccaaaaggat gattaaataa
    12241 taatacacaa gctccagttt ctggaatata tgataagtct tgtgggtaaa ctttaatttt
    12301 attattttta tatttaaggc aattttcaac aaacttttcg ccatctacat caaaacagtg
    12361 attatacatt ttgtttagag attttaatcc caaaacactg tataatatgc ttgatataaa
    12421 tttgccaaat atacccttta gctttacaac taactgtata tcatgctttg ttactacact
    12481 atgccttaaa ttagtcatat agttttttta gttcacaaat atatgtaaaa aattcagcaa
    12541 ataaaaatga aaaaaacgaa gtgttttaaa gaaaacatat tagttaaaac caaacactaa
    12601 gatttagaaa gaaaaaacaa atatcgctat acaaaacgcc aacttaaaaa gatcaaaaaa
    12661 ttcacaattc tatactcaaa ccgtcttcag caattattgt gttttcaaaa acttcttgcg
    12721 cttcttttaa taaatgattc atatttttat aacgaacgga aaaatgtcct atcacaagtt
    12781 tttttgcatt aactaatttt gcaatttctc ctgcttctct tgcggtagaa tgtaatgttt
    12841 gctttgccac atcagccaaa tcgtttccaa aagtagcttc atgatatagt aaatcaacat
    12901 tgtaaatata tttatccaaa tttcttactg gaattgtatc agtacaataa gcataagacc
    12961 ttggtataga agcagcatag gtaagttctt gattttttat taatattcca ttttcatcta
    13021 taatatcttc tccatttttt aattttacaa tttgtgaaat acttaaattg tgtttttcaa
    13081 ttgcttcttt ttttatattt cttggttttt ctttttcttg aaacaaaaat ccccaaactt
    13141 gcttactatg ctttaaaggg aaactataaa cttcaagatt tttgttttcg aaaattaaat
    13201 tttttccatt tgaatttaaa ctatgaaaaa tcaatttata tttaagttcg tctccattaa
    13261 ttttgtgtaa agagcttatt atttcttcaa gttgagcagg agcataaata tgcaggtctt
    13321 gttttcgtcc caataaattc aaacttgata tcaagccaaa aatgccatag aaatggtcgc
    13381 catgtaagtg tgaaataaaa atatttttta acctcccgaa tggaatggaa aactttctca
    13441 tttgcatttg agtaccttcg gcacagtcaa ttaagtatgg cgttttatta tacaacacca
    13501 tctgagcact tggcattcga gttgaacttg gtaatgcaga gctggatcct aaaattttaa
    13561 tttcgaactg cataaaaatc ttttaaaata taatacctaa agggatacta atttcaacac
    13621 cagcttccat atttggcaat acatattgat ttttcacact caattccgac aaagcatatt
    13681 tataatatac tgaaaacatt ccataaccaa acttagccgc taagccatat tgcaaattat
    13741 catttattga atatttttct aaagatttta cttttgtatc tattgtttta tattttaatc
    13801 tgaaatttgt agaaaacaca caatcagcat aagctccaac atctaaatga agtcctaaac
    13861 cactttgtcc agcagctaca aatcttattc ttccgtaaaa ttctccagta aaagctttaa
    13921 gttcaaaatt ttccttataa gaaattaaag aaactcgatt attattaaca aaatcggaaa
    13981 aatcatttag gtgaacttta gattttgaat atgaaaaatt taatcctaaa gaaaaagcat
    14041 ttacaatttt ttgtttataa cgaatgccaa aagtgaaatt tccagcactt gacatatcat
    14101 aattatctga tttatcgccg aatacatatc cttttctgat gaaaggttct atataaaaac
    14161 aattgttcgc tccaaatttc gaatactttt cttgtcctgg cttttggctt cttcgtgatc
    14221 tagttcccac atagcttgga ttaaaatcat tatcaacata ttctttataa tttttaagtt
    14281 tatattcttc attatagcct tgataccaaa ctgctaaaac actattttcg tctgggaaag
    14341 ctgatttagg caaacttatt ttagagtttc taagtgaaaa aacttttact tgctcataaa
    14401 aaactaattt tccttctgaa tttctgatat gatagaacac atatcctaaa tctttctcct
    14461 cttctgaatt aagtaatttt atttctacta tattttcatg ttcttcgatt tcgaagcttt
    14521 tagcaaaagt tttgctttca ttcaagaaat acgaagccat tggaattcca taaccatttg
    14581 cataatcaaa atatggatat ttatcagcag atttttctat ttctagtttc atttcttttg
    14641 cactcaagtc tggttttgtt tgccaagcac aagccacaaa gcctgcaact aaaggacttg
    14701 caaaagaagt tccatatagt tgtccataag attttgtagt ggctgtatat acgcttccat
    14761 aagccgaaac gtttggtttt agcctgccat cataacttgg tccgtaagaa gaaaaaccta
    14821 tatgagcttt accctcataa tcaacacctc ctactgcgag tacactttct gagtcggctg
    14881 gtgtaataat tttttgccaa gatttgtctg ttccttcatt tcctgctgca tttacaacga
    14941 gaattccttt ttctaaagct gtgtttgctg cttttgcaac tacagaagtt tttccgtcca
    15001 tttgttttag ttttcgtctt tctttgccat atcccaaaga tgaatttaca atgtcagcac
    15061 catttttatc tgcccactct aaagccatag cccaccaaac ttcttctctt gcaggctctg
    15121 gtcctatttc ggtacgagcc aataaaaatt ctgcacctgt agctaaacca atatttttcc
    15181 catcttcatc aactcctgct atacatgcca aaacacttgt accatgcgaa ttccacccat
    15241 aaacattttc tattttacgt gggaaattcc atgtttttac aatctgatta ttatccctca
    15301 aatgtttaaa agcttgatga gtgtcaacgt ttggaaagcc tccatcaaaa actgctattc
    15361 gcttgccttt accattaatt cctttttcga taaatttatc cccttccatt ctttttattt
    15421 gaggaagata aactttagcg gaatcatgct cttctgtaaa aacaccttta ctttcacaca
    15481 aagctaagtc attgtcaatc aactgaatat ccaacacgaa ctcgaactta gaaataatat
    15541 ctattcttat ttcatcagta taaattgccg aagcattcag ccaacgactc tcgcctatat
    15601 attcatcaca ataatcagat atagttgcaa cataagtttc gtttaaagga aaatcgctta
    15661 aatcaaaaag agaaattccc aagttttccc tcctctcaat agctttggta tcaaaatatt
    15721 tataagggtc aaagctagtg tttgctttat ctttataaaa tacccaataa taattttggg
    15781 aaaataaaga gattgacaaa cagaaaagaa cactaaaaaa tagtaaatat ttcatattat
    15841 tttcaaaaaa atttaaggtt taacaaattg tccgataaac aaaggcgtat tgtatttatt
    15901 ttcttttaaa acataaaaga aaggtctatt tgcgataaac tcataagttg ggtttggatt
    15961 ttgtataaaa gcagtcgttt gtacaatcat tgctgttgca gcagcagctt ctgttccttt
    16021 ttcatcaact tcaaaaaata ctttttgaag aacttgacta atttgcaaat tattattttg
    16081 agaaattcct gaaaaatcag cattagaaaa tgcagaactc attcccattt ttttcaacat
    16141 ttgagacaaa ttaaagctag attctatttt aaatttagga atttcaatat gcacttcttc
    16201 gtaacgaagc gaattacaaa tctttttata ctgctcgcca tttaaaacta catcagaaat
    16261 attatcaata ttactttcaa ctggcaaaat aaacaataaa gataaatcat tactaacata
    16321 ttccatttca atagctttgt aagaatttat ttccgcatat tttatctttt taattttttg
    16381 tatcataaaa tctgtttcta acttttcttt tttacttaaa aagaagtcct ttttttgggt
    16441 tctttccttt ttaaattgat gtttccaatc agaatttata taaattgcat taaccaatac
    16501 cattgcagta cttggcgaca atgtcccgtc aggaattaaa tttttaattc tattatttgt
    16561 tagtttttct acccaagaat ttatattttt tgcttctttt tgtgcattgt taataaaatc
    16621 tgccaattga aaagctccat caaaatattt ttcaatctta gcctgatacg aagaaagtat
    16681 gttgtaagtt tgttctaaat aaattttatt agcaagccta aattctacca aactatcttg
    16741 agctatctta tccaacaaat ccatatattc agaaatgcta agtaaaaaat tttgcttgtc
    16801 gcgataaaaa cccataacat tactaatatc gtcggcagtt tttccttcag caccagcata
    16861 agccattccc atagctaaat ttaacgatac tggcgaaaaa gaatagtttt ttctaggttc
    16921 gtcttttatt aaattagaat atagtttaaa agaaaagtta gtcaattttt gattttgatt
    16981 agcttcaatt tttgctcctg tagttgacat tccagaggag tttttcattt cttttttagt
    17041 tttacacgaa aacaataaaa aagttaaaat aaatgaatag caaacaattt ttttcataat
    17101 cacagttttt taattttatt gcaataaata tgccctaaaa ttactttatt tttttaattt
    17161 ttaaaaaaga ttttacaaaa cacagaaaat gcacaaaaat taactaattt tgctaaagat
    17221 tttataaaaa aatgaaaaaa cttattcaag acttaaaagt tgttagcaat ataaaactta
    17281 atgctaatta ttatttatta aaattgtttt cagaggaaga tttaccactt attttgcctg
    17341 ctcaatttgt tgaaataaaa attccaaatt cgccatcaac atttttgagg cgtcctatta
    17401 gcattcatga tgtaaattat gaaaaaaata caattaagtt attaatcaat attgctggca
    17461 aaggcacaga gtcgctatct aaaattcaag ccaatgatta tttaaacata gtttaccctt
    17521 tgggaaattc ctttacaatg ccaacttgtg ataaagtttt gcttgttggt ggcggaattg
    17581 gtgtagcacc tcttttatat actgcaaaat actttttgag ttttggtata gaatccgatt
    17641 ttttacttgg ctatagaagt aaaagcaata ttgttttgca cgacaagttt tcaaaatatg
    17701 gaaaagtttt ttacacaacc gacgatggca gttttggcga agaaggtcat gttttaaatc
    17761 actctatttt cacaggttct gtatttaatt ataaaaagat atatgcttgc ggtcctaaaa
    17821 ttatgctaaa aaacttgtct atttgggcta atgaaagaaa tattgactgc gaagtttcat
    17881 tagaaaactt gatggcttgt ggtgttggtg cttgcttgtg ttgcgttcaa aatacagttg
    17941 atggtcataa atgtgtttgc actgaaggac ctgtttttaa ttcaaaagaa attatatggt
    18001 aaacttagaa ataaattttg ctggtttaaa gtttaaaaac cctatactaa ctgcctctgg
    18061 cacatttggt tacggaacag aatttgctga ttttatagat ctgaatttac ttggcggttt
    18121 tattgttaaa ggaacaacat taaattatcg cgaaggcaat ccttacccaa gaatggcaga
    18181 aacagcttcg ggaatgttaa atgctgttgg tttgcaaaat aaaggcgtaa aatatttttg
    18241 tgaaaatatt taccctgaaa taaaagattt aaaaaccaat atttttgtaa acgtttctgg
    18301 ctcaacaata gaagattatg ttgcaactgc cgaaatgata aacaatttgg aaaacattcc
    18361 tggcatagag cttaacattt cttgtcctaa cgtaaaagaa ggtggaatgg catttggcac
    18421 tagttgcaca tctgctgctc aagttgttaa agaagttagg aaagtttaca aaaaacattt
    18481 aatggttaag ctttcgccaa atgttactga tattgtatct attgctcaag ctgttgaaag
    18541 tgaaggggct gacagcattt ctttaataaa tactttttta ggaatggcaa tagatgcaaa
    18601 aactcgaaaa ccaaaattat ctacaattac tggaggatta tcgggtccgg caattaagcc
    18661 aattgcttta agaatggttt ggcaagtttc taaagcggtt aaaattccaa tatgtggttt
    18721 aggtggaatt atgaatgccg aagatgttat tgagtttatg cttgctggtg cttcatgcgt
    18781 acaaattgga actgcaaatt ttatcgaccc cgacattaca attaaaatca ttaatgattt
    18841 gcaagtcttc tgcgaaaatc aaaaaataaa aaatatagat gaaattattg gagggttgag
    18901 attgtaacat ttagtatatt cactcttata tcccaaaata ttgtttaaat tcaaaggttc
    18961 cgcttgtata agttgtatct aatttttctt cttgcacatt tggaactaat atttcagatg
    19021 caaaagttcc actaatgtaa tgtttacctt ttttcttgtc gcaaacacct gtgatatgga
    19081 aaggtccata atagataaga ccttccccac ctcttatgtc tccatgatgt gttaatatac
    19141 cagtaatatt ttttccatct ttatacaatg tttctttata tggcttgatt actatataat
    19201 ctcgtgttga ctccacaact tgggaatgac ttaagcgtgc ggtaagattc gagccataat
    19261 tcttaaaagt aaaactataa taaccttcag gtatcgtccg tttctcgcaa cttaagaaaa
    19321 acattaataa tacaacaaaa cttataagca catcaatttt tttcatattc taagtattta
    19381 aaggctaata tttgattatg acaatttatt ttatatggta aaattagaga attttcaaat
    19441 ataaagcaaa taaaatttgc atatttttta tatgtcttta tatatgcgca aagatattca
    19501 ataaaattac tcttgattaa acgacaatac agttgaaagt taaaagcgat gcactaaacg
    19561 tagttgaagc gttgaaagta taataaaaaa tttgtatagt ttagagacgt tatcacaacg
    19621 tcccactata caaatttcag atattgcttt ttgtcctagg acaagacttg gtttattttt
    19681 tttattcttg aactaaatca atattaattt cttggttagt tcgaatatcc atttccattt
    19741 caacagattg ataagaaata aattcaacct taacagtatg ttttcctggt ttaatatttt
    19801 ggatagtaaa atttccgtct aaatcggaat agtattttgt tccatcaata taaatacttg
    19861 ctcctgctaa agactcatta ctttttttat ctgtaattat tcctgataca ttaacaaaat
    19921 tagctttttc aacagcttcg gttttgttag tttttacatt tttgcttgat ttattattat
    19981 ctgcataagc agtaattgat aagaaagcaa ttgcaataat taagaaagta gttttcataa
    20041 atgtttttaa ttattattta acgatacaaa aatgacgtat aagtatttca taaaaattac
    20101 atcaaaatta agattctgtt acaattttat ttttataaaa acatattaat attttaatat
    20161 tgtttttcgt ccacaattgt acctttttca tcccacatac gccaaattcc tgttttttct
    20221 ccttcgtaat aattcatttc ataacgttgc attcctaatt catcccatat acgccaagtt
    20281 ccgtgttttt gtccgttttt gtattcggct tcagaaatta gctgtccaga aatatcataa
    20341 tttctccata aaccatgaag ttttccttct ttgtatgagc gtatttcttt gggtttacga
    20401 ttttcgaaat aaattatata agttccttct ggcaaaccgt ttttaatttg catttcgagt
    20461 ttaagtgttt tgttttcgta atattcgcga taatcgccag tgtatagctc gttttgtttt
    20521 ttatcacgat agtaaacacc ttcgttttga tatatttgag gagttttttg agcttgcagg
    20581 ctaaatacca ccaaaaataa cactgtaatt aaaatcaatt tatgtctcat ttgttcctaa
    20641 tttattttat agaacaaagg tatctcgatt tttcgaaata cctttgattt ttgcttaaat
    20701 ttttaaagta aaactaaaat gtatatcctg tccagtcgaa aatatttgtt gggcaacctg
    20761 tattaccatt ttcaattttt acattatcat atgtagttcc gtcttttttg gttatttcgt
    20821 taccactgat tggtgttgtt aaagaattag ttatagaaat tgttgcgttt ccgtgtcatt
    20881 gtcattttac tttatttctt ttctgtcggc tggtgtgtcg gcgtttttta ttttttttaa
    20941 gtgtcggcaa attttttaaa acaatttctc tcggtgcgtt ggctttgcta gctctttgac
    21001 aaagttttgg ttagattttt ggctcgtgtg gctttggcaa tgtgcttgca aaatgcgttg
    21061 gctaattgca aagttagtta tattttcttt atcaaaatct ttttcttaaa aatgttcgta
    21121 tatttgcttt ctatttgtta cagaacagat tgaaaacgaa ctgaatttat ttacttttaa
    21181 aaaataacaa ttatgagcat gaacaaagca caattaattg atgcaattgc aaaaaagggc
    21241 aatatcaaaa atgcagaagc aaaaaacgct ttagaattaa ctttaggagc aataagcgaa
    21301 gctctaaaat caggtgaaag tattactctt atcggtttcg gtacttttaa gattgtcgat
    21361 attgctgaac gtactggaag aaacccacaa actggtgaaa aaattcaaat taaagcaaaa
    21421 aaagtggtga aatttaaacc aggtaatgaa ttgtcaggcc atgtaaagta attttgtgaa
    21481 tgcaacttga tttatagtct tttaggctta aaaataatag cattgtgaag aattcaataa
    21541 catgtatgac tttattatgg agaaaacaat aagactaaac ttaattaact atctttataa
    21601 agtatttctt taataaactt aacattatta gacataaaaa ttattgatac aatttcttgt
    21661 tttgatgtta agatattcat ttttttaaaa taattcgact tgttataatg tttaaaccaa
    21721 atgttaaata agcgataacg agctttatca agataatctg cattatcata aacaatgaag
    21781 attactcttt cagggttagc tgttatgaaa ttatatataa ccttacaaat tgaatctcta
    21841 attagtttat tgtttatcga atttttatcc aaactttctg ttttatcaaa ttcaaaaata
    21901 aatttatcac cacctaagtt gttaaacaat aatgtatatt tacatttatc tgtggcatta
    21961 aaacaataac caaatccttt cggattgaca aattcattta tttcgtattc attatgcatt
    22021 ataagtcatc aggattataa ggattgtaaa agcatgggct tgtttttatc cattctccat
    22081 tttcatattt taaaggaata ttatcaacac acatatatct aaattcacag tcttggcaga
    22141 tttttatttt atcattgtta atattccaaa gtaactgaaa atcattagtc tgaacaatcc
    22201 tatcaatact gtctaaattt tttacataac caaaagtctt atttagagat aaacaatttt
    22261 ttatttctcc ttcttttgta atgcaaactt ttctgttata ataattattg aaaaataaac
    22321 tttcacaaaa aagagtaata ttgactttaa aataatattt gttattaaca tgaaaatctg
    22381 aatgtttttc tttcatttca cttgaaaata caatcttttg tctttctatc ttattgtctt
    22441 ttaaaacttt gtttttttct gctccataaa agtataaaag tcttagtctt tgatgatttt
    22501 ttaatatact aattaactta tcaatatcgt aatttcccca ctttgatatt atttcaatat
    22561 aatcaaagtc tttattatta agaaacgtaa gtattgcttc tatataattt aaatttaagg
    22621 tattaaaagc tctaaactga ataaatctac atcctaattt ttctaagctc aatagagata
    22681 aagttaatgt gtcattttgc ataggaagtt ctaaaattgc attagagact tgaccagggt
    22741 agtcccatgc tggttctaaa tcaacaaata attcaagttc atcttcatca caaaaaaaga
    22801 taaattcatt ttcaataagc atcttaaaat attcatcaat aattgaatgt tgagtttttt
    22861 caaagtctct tttaacatca tcgattgtat tggttttgta tgttgataaa atatcatata
    22921 aaccattagg aattagtttg tacgagttac gaggcaaatc gtaaatagca cttcgtttat
    22981 tgcctttgac taatttgcat tcagcaaaca atttaaaata cttactttca gaattcattt
    23041 ttaagattta tgataggtga aatgatttta taaaagtttt gcggataata atcacttgta
    23101 ttatttgaag ctttgattat tttcaaatga tcatctttca acttttcttc tattttaaga
    23161 aattgaacat ttgttggtag cttgtcaaaa tctttatcgt ataaatcttt aatactctta
    23221 ctttccattt ttaaaatatt tagcgattct tttttctaaa tagaaattgc aagggtaaga
    23281 aacttgataa aactgtccta ttggattgac ttccaaaaaa taatatcttt tgtccttgcc
    23341 gactaaaatg tctattgagc cagatttcat atttgttttg tccataaatt ttataattgc
    23401 taattttaaa ctatctggtg ctttgtaagg aggtgtacgg tttggtttct gaaaattata
    23461 attgcgaaaa tcaatttttg tcatttcgtc caattgagaa aaaattgcgg aaatgtatat
    23521 ttcctcttta aagtaaaaaa ctctaagctc ataggctttg tcaatgtact cttgaaataa
    23581 ggttggagca aaagtatcgg gaaaattatt tattatctct tcagtaaata aataagattg
    23641 tccagaaaaa catatctcat cattttcata atataagcca ctttgagtta tgggttttgt
    23701 aagtaatatt ttatattctt taataatgtc ttgaacttct tgtttatttg tcaatatcca
    23761 agtccgtgga atattcatac cacatttatt tgcaatttta agattaataa gtttattggt
    23821 attattttca tatatatttc cgacactctt tttaatatta tgtaattcat tataaataaa
    23881 ttcagtaatt gcatcaaact catttagtaa aaatctatta gttacttcag aaaggtttaa
    23941 atatttttgt tttccaactg catttacatt taaagaaagt ttccctctac ggtaccaata
    24001 acctgtaatt ttacttaatt gaattactgt atcatcatgt tgacaaacaa aatcaatgtt
    24061 accatcggca tttatagtaa gtttaaaaaa atcaaaaaca ttagtattgt tgatacgtaa
    24121 gtattcaaca ttataatatt ttaaccaatt tattacacta tcagttgttg catcatcagg
    24181 ttctgaaatt attaatatca ttgttaaaaa cttataaaaa atttattcaa gcattatttc
    24241 atcattggct tttttatttt ttcttctttc tctttgtatt agttcatttc ttttttcttg
    24301 ctccactgtg tttagtccca cttctagtct ttttctattt gtaatggtat cattaccata
    24361 ataactttca taatcggtat ttttactatg tctataaata tagcgacata tatttgcgta
    24421 ttgaaatgga gtcatgtttc catttatcac attttgaaat aatgtatttt taaaatcaac
    24481 tgatacgtta cgctttgttg aaaagtaatg aagcaacata aaataggctt gccattctct
    24541 aatataaggt tcccaaaaac taatatatct tactgttgat acagaatctt cgtaagtttt
    24601 gggcaatcct ataagttttt ctccgggaaa cccatattct ttgatgatat tattaattat
    24661 tgtaaattgc tttttgttat ttattatcca tttcaaacca aatatagtat gcctgaacag
    24721 gataaatcca tcatttacct tttgtgtata tttttgatct atttcaaata aactgtctat
    24781 ttgatttgca agatttaaat ttattgatgc tttataaata gaataaagac tatcaaaatc
    24841 ttggatagtt tccatagtag tagagtatgc gagtgatttt tttgttaact cgcttgatct
    24901 tataaaccat atcgaaattc cttgcgtaaa acacttatta agccacttat tagccctgat
    24961 gctatcatca acataacaac aaatttgtaa agacttaaaa cagtgttgtg cataaataaa
    25021 atcataattt gtataaatac tatccaaacg tagcagggct aaattataat tttctgacaa
    25081 aacatcttca tctactcggt taaaagtttg atgatagtat atataatttt gtccaaaaga
    25141 acataacgat atagctgaaa ggatcaacaa aaaaatcact aatcgcataa ttacatacct
    25201 tgtatagcaa aaaaacgcat cataccacct ttaaaagtag tttgagtaag aaataataac
    25261 aaaaatatca ttcccattgt aaagaaaatt attactaata atattcttta cagcaaggtc
    25321 cttcatattc catcaagtct ccagagacaa ctccattgga gtttttatta tagtcgtcaa
    25381 ttgcttttaa aacagcttga gctgcttgtt cattacatcc atcatctttt cttattgtat
    25441 cagttccccc ctcaggtcct gcagatgaat ctgtcctata ctttgatgta tcattagagg
    25501 caccagtgca atcataagta caacttgagc agtcttcatt ttttgatgta gctggagttg
    25561 cagctccacc aactacttct acaagttcac gcaattcatt tgataaaatt tcttcagaat
    25621 taaataattc ttcgtttaaa ctttgtaagt ctttcttttt cataacgata aaattttaaa
    25681 ttaataatag aaaacacaaa tataagaatt atttgcgata tttgcaaaaa ttatttttta
    25741 tttggtattt ttttgtactt ataattattc taattgaaaa gattcaaaca cattaaacaa
    25801 aatgaccata atgactgtgg accgacatgt ctaagaattg ttgcctattg gcatgggaaa
    25861 aattatgaca tagattttct caaaaaaaac tgcaatatta acaatgatgg tgtcaatttg
    25921 ctagatattg tcaaagcggc agaacttatt ggatttaaaa cattatgtgt aaaaattgac
    25981 acaaagaaac ttgataaagt cttagaaatt ggaccttgca ttatacactg gcagaagaag
    26041 cattttgtag tactttacaa acaaaaaaaa tcattttgga aaagaagtaa gatttattat
    26101 atttcagatc ctgccagagg tcgtttaact tttaacgaag aaatctttag taaatcatgg
    26161 aaagggaata gtgcaaaagg atattgcttg ttattagaac caaaggaaga tttttacaac
    26221 ttgaaatttt aaatcaatga attaagataa taatttttga aaaattttcg aataggcaaa
    26281 atttgattct tttatgtgtg atgtaaaatg gcatttattt aaattcttta atgatctatt
    26341 tacatgtaaa acgtgcgttg taaaaatttg gctctttgac ttttgcgaag ttttggcacg
    26401 tgtgtcgggc aaaaggcaat gtgccaaatg tgggcaggca ttaaaaattg ttttaaaaaa
    26461 tgtgcgttgg caaaaaaata aaaaacatcg ggtcggcttg tgtgtcggct atcttttctt
    26521 gtcaatattc cttttttctg tcctcctgtt ttccgcattg ggacacccaa aaaaataatt
    26581 cattaataat tttataag
//
LOCUS       DAOPNX010000025        26112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_2392_length_26112_cov_6.152013, whole genome shotgun sequence.
ACCESSION   DAOPNX010000025 DAOPNX010000000
VERSION     DAOPNX010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 26112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26112
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2392_length_26112_cov_6.152013"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..677)
                     /gene="yihA"
                     /locus_tag="PKN53_04245"
     CDS             complement(90..677)
                     /gene="yihA"
                     /locus_tag="PKN53_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOD88196.1"
                     /translation="MQIKTAEFLMSNQDYRNCPQDDIPEIAFIGRSNVGKSSLINMLT
                     NNSNLAKTSSVPGKTQLINHFIIDKSWYLVDLPGFGYAKVSKTVREKFKKMLEDYILK
                     RPNLVCLFVLVDSRIPAQKIDLEYMYWLGLNSVPFKIVFTKTDKLGVNALKKNIEDYK
                     LSLEKYWENLPDYFITSSTTKRGKEELLDYIESIL"
     gene            complement(680..2029)
                     /locus_tag="PKN53_04250"
     CDS             complement(680..2029)
                     /locus_tag="PKN53_04250"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOD88197.1"
                     /translation="MLTFKKITFLCLSVFLSIYSIAQYKLSCRIENYAHREVKVFTLF
                     GDEAEYVESVQTNINSAFEFSFDKKEVGLYRLYLDNNEYFDLIFNNENIQLSTFVENP
                     VYNMKVIESNENFQLYEFLKMEFMYKYKIQILEQFMSIYPDETFTKTVEKEKAKQLKE
                     KNDLLIKVINLNKNSFAGKYLTYFKEVTAPEKLSIEKKQEFYRKEYLKSYDFSSVLML
                     KSNAYQKVVLDYLKLFRATDADVYYVAGKDVLDHIFFEEPVIFNTIFEFILNGFETIG
                     LVEQSSKLSLEFGDLCSDASDNLKARIKANTELTIGKKAPDFTVQTLSGQTYTLSKMK
                     SNYTLILFWATWCGHCQLTIPTFAEAQQLFDAASVDIIAISLDKDKKELNEFLSKNPF
                     PWNIACDYKSWDGKQVIDYSVYATPIMYLVDKDLNIVAKPYDEQRLFRELEKILYRN"
     gene            complement(2263..4680)
                     /locus_tag="PKN53_04255"
     CDS             complement(2263..4680)
                     /locus_tag="PKN53_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88198.1"
                     /translation="MRKIFTSIVCLALVFSVNVQAQKTFMEGDILSSDPINPENFNDK
                     LFQDVLVYKINTYMDSLGLEGFEIHDFFINPAREHATYMAETGEANLTGSGRAGTVRD
                     RLAYAGGTGVGAEVVARANIRTANEYISYDELANQTLDKWKTGKYSKDLLSQKYFFIG
                     ASGKVDASQKRVFVSVYMGNYASFISGAGNVLELAAPISVKSQGLKVYDEKICKKTTR
                     KMPDVVDLQEGLSINDNGEIVFKYNDLRKFRRFIRASKDGLAVDIVQKDQFNRCKSEN
                     IVDYSKVNIGFMTKKMFSKKIYKKNIAEGEGRRKKVTKLEVVLGQLPAGFDPQNIELN
                     LMVIKEKHVCHNIPQSWVDTKIYDFVPKIALMPDTILPAGISEYAPTATSTELNFRIP
                     FEQGKFTYQPDDMKPVLAALNEPDFIINKIFIEAYSSLEGSIAENAALQKRRANSIVK
                     ALEENQNASIVDSVITAPNLKDLQNDCKGTIFEDVCDMDLEQAVRYVNSKAKEMEMFL
                     ENHRYANVTIWITYDIDGEKEQKYVVTQFNRAVEAGQINTALAIQKYILKRVVEGRYN
                     ENVVADMKIPSGKEYVGLNMNKIWLTQFIYMDVLDEDYLTKIDDLNKLDQTNIYVDFN
                     DVLCKVTLTDLDNDRTQQTLQNRIDKMYNTSLRVDLVDLLNIELQYQIMDIYKDSLGY
                     DHPSVVKAMNKMKEIIHIDELTWANSLKLASVFINHSDYEYAIRLLEPWIKDESIPFV
                     YLTTYATVCSKVDYKVHSNNFVYALDRIRKQDPEFLCDLFKGDKLSVQTFVNTRVKQL
                     YCETCKK"
     gene            4987..5367
                     /locus_tag="PKN53_04260"
     CDS             4987..5367
                     /locus_tag="PKN53_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOD88199.1"
                     /translation="MEENLRIDKFLWAVRLFKTRNLAQEKCNKGHVLINDNAVKPSKN
                     VKIGDVIFIKNPPIIRSYKILKLTDKRVSAKLVPDLIVETTPQSELDKLKNIETAFII
                     RDKGSGRPTKKERRLINKIQENNL"
     gene            5364..5891
                     /locus_tag="PKN53_04265"
     CDS             5364..5891
                     /locus_tag="PKN53_04265"
                     /inference="COORDINATES: protein motif:HMM:NF027596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4924 family protein"
                     /protein_id="HOD88200.1"
                     /translation="MIIAEQKYKENIIEYIIYIRQIQDIIRLANCDIEEINKLIIDQY
                     KTSEKIKIKIRDWYLDLINLIKTEKIDKQGDFGFIKNIIDELEELNQKLLNQENSYKH
                     QELYRWAKPNIEEFKKLSNNANEGDVKTCIDALHSLLLLRIKNQPISSETMQAMQTFS
                     NLMANLAFEYKKLKS"
     gene            complement(5892..6926)
                     /locus_tag="PKN53_04270"
     CDS             complement(5892..6926)
                     /locus_tag="PKN53_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88201.1"
                     /translation="MKKKYYVLILIFVLLVGILMFFVFSKKKAEFEVDISKIELNIEI
                     ERFDKDFERFSKGDFYTQVAFMEKTYQEFFEVYNYEIIAIGGADNSAYLSYVNTFLND
                     FSVVQARKYVEKEYSKLDDVNEDLTYGFKHLKYYYPDVKIPRFVSFIAGFNQSVVLTE
                     DFIGIGLDKYLGNDCELYDMLQIPDFSKSEMTREQIAIDVLSAWAEAEYPFYSDSENL
                     LENMIYNGRKLYFLHSMFPNFKPERINKYTKEQLDFCEKFEREMWTTIVENKLLFSTD
                     ALTIKKFVGSAPFTYQFGPDSPPRTGNWLGFKIVCSYMKNEDVSLEELMEEVDYQKIL
                     NLSKYNPKYK"
     gene            6998..7687
                     /locus_tag="PKN53_04275"
     CDS             6998..7687
                     /locus_tag="PKN53_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOD88202.1"
                     /translation="MKKIILILISLFFFGNIFAQQAISGGLYIGPTMSWMSTDSKVAD
                     TKGMKFGYNFGALVDFNLIDNFAISTGIQFNNLGGNIAYKYGFTNLKPKEGAVIDTVF
                     PGTKIKYNLNYIGVPVSFKGKTNEIGYMTYFLKAGVTPLINIKTLGSFGENEKLMLKE
                     INLFNVAWHIGGGIEYSLSGNTRILVEILYTGGLIDVDKTKVFDVPDMTSTSNPRIAL
                     NDIHLKVGVLF"
     gene            7798..9459
                     /locus_tag="PKN53_04280"
     CDS             7798..9459
                     /locus_tag="PKN53_04280"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOD88203.1"
                     /translation="MKIAIAQLNYQVGNLDFNSQKIINTILTAKNQGVDLVIFSELSI
                     CGYIPYDLLNYNFFIDDCYKKLEEIKLQCTNIAAIVGLPTKNLKNTGKKLYNSAAFLY
                     DGEIQKVIHKTLIPNFDIINEDKYFEENQDFSIINFKNKKIALTIAEDLWDEEKIHGF
                     FENKIMFKNAPMEYLNKFNPDFIVNIAAQPFAYNQTDCKKDFIYKNAKKYSIPIININ
                     QVGANTELIYEGASIVVDKTGNTIISSNRFKEDLKIFEFDEITNTSQTNNHNRNSDDK
                     YESIYSALILGIKDFFEKSNLKKAVVGLSGGLDSAVVVALLVNALGKENVDVLLLPTK
                     YSSQHSIDDSLEMVKRCGIKHHIVDIEELRKMYLKTMEKVFENTKEDITEENIQARIR
                     GSVLMAYSNKFGNILLNTSNKSEAAVGYSTMYGDMNGALAVIGDLYKSEVYELADYIN
                     QNFNNMIPQNIIDKIPSAELRLNQKDSDSLPDYEILDKILFLYIEQNLSEEEIIKKGL
                     PQEIVHRIIRLVNSSEFKRFQMPPVLKISRKSFGDGRKMPLVGRF"
     gene            9497..9799
                     /locus_tag="PKN53_04285"
     CDS             9497..9799
                     /locus_tag="PKN53_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88204.1"
                     /translation="MEDRTFQEILDFSILELGSEFNLTVLNLFELVLLFILTFLALFL
                     IKKTIYKSKKLDLAKKYSLNNLTKYVVYTIAFIIFFNILGFELKYVMAGSAALLVV"
     gene            9817..10272
                     /locus_tag="PKN53_04290"
     CDS             9817..10272
                     /locus_tag="PKN53_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOD88205.1"
                     /translation="MFSDFVSGIIILIDSSIKVGDILDVDGLICQVEKINLRTTLVLT
                     RDDKFILLPNSLLTKQKIVNWTHSKNISRFEVDVSVARSSDVELTMKLIKEAGLEQKD
                     VLKDPDPFVRLNNFGTSSLDFTLYFWVNNVFRVENTKSAIRVILYKKLK"
     gene            10490..11680
                     /locus_tag="PKN53_04295"
     CDS             10490..11680
                     /locus_tag="PKN53_04295"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOD88206.1"
                     /translation="MKFKLKLKHYTLYLGLIAYIIGIALNIFYSLYSNQPGPQQSQSP
                     LFEILSLFPPIIILALTLVSASIFEELAFRGWTIKHKFSKYISIIGILFYCFLATNSF
                     IICVIVLALLIFVFFYKNDELSQFIKVIVTSLIFAFIHFDNFSDNMRYISILQLFGVS
                     LIISYFALRFGFVYGILIHFANNLLATIIIFTTSANYSTSIVKENYMANIEKIEFLEN
                     SSSIQEGSFQDSIVHVGNITHIAVELAPFNNEVIYKAKLSSLDSYHYVAKCKNKETCT
                     LDRQELLKDFLNHTNLKTDTILVDAFVLKIIDIDKLQEHRADGHTTLIKYLVEGLRSE
                     PFNIPIFIDEDIVDISFIMPDNFYRVFLKLSTKDKIKKLETDYGILLEKTNDKTVSVV
                     YFFE"
     gene            complement(11702..12067)
                     /locus_tag="PKN53_04300"
     CDS             complement(11702..12067)
                     /locus_tag="PKN53_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88207.1"
                     /translation="MKKIIIPAILYSLILAFFACSTIYNYDLSIGGLDDLPATKACLT
                     KYLPKSVDAQKGQQGREIAPITLIFDEHEAEIKDSLLADSVVFEDNSYLLKFKIVALD
                     PFDEVTEYDVEKQYFLENE"
     gene            complement(12064..12795)
                     /locus_tag="PKN53_04305"
     CDS             complement(12064..12795)
                     /locus_tag="PKN53_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOD88208.1"
                     /translation="MNRLKHILILLFVFIINFKFLLAQTYKPAPDFSLLDTNGEEVNL
                     YDSIAAGKVVLLHFFSLNCGSCIEVAPIVDSIHRQYGSGTQDLIVWGIVHYYYNSEEI
                     EAFKEYHNLTFRCFPTGHATDVFGLYEINYTPQIMMVCDYIVSESLSYFNIIETLNYC
                     LPSSKLEEKNNDFTFNIVGDFLNFESQKKVSEIQVYDYMGRLVFNEKNPKNFIRIPLL
                     ISKSLYIVNFVFDDNSKFTSKIISQ"
     gene            12974..15199
                     /locus_tag="PKN53_04310"
     CDS             12974..15199
                     /locus_tag="PKN53_04310"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOD88209.1"
                     /translation="MKENMFWLVPFASILALGFAWYFFRSMMKEKEGTHMMKRVALYV
                     RKGAMAYLKQQYKVVAIVFIALTLLFAIMAYVLKIQNPWVPFAFITGGFFSGLAGFLG
                     MRTATYASARAANAARHSLNASLKLAFRSGAVMGLVVVGLGLLDISLWFYVLQYAIGA
                     LDYTTNPLIAADPSMKLIIITTTTLTFGMGASTQALFARVGGGIYTKAADVGADLVGK
                     LEADIPEDDPRNPATIADNVGDNVGDVAGMGADLYESYCGSILSTAALGAVAFKSSGL
                     DVQFNAVIAPMVIAAVGIILSILGIFLVKTKEGANQKQLLAALGRGVNVSSILIAFFA
                     LGVLYYLGLPNYQGIWIAMLVGLLGGIGIGKSTEYFTSSGFKPTRKISESAKTGHATV
                     IIQGVGTGMISTAIPVLIVTASIILSYLFAAKFDFSNIGMGLYGIAIAAVGMLSTLGI
                     TLATDAYGPIADNAGGNAQMSGLEPEVRQRTDALDQLGNTTAATGKGFAIGSAALTAL
                     ALLASYIEEIKIAIGRLPEKASEFMSTTGVDIHSATIPDIMSFFQVNLMNPLVIVGIF
                     VGAMTAFLFSGLTMNAVGRAAEGMVNEVRRQFREIKGILTGEAEPDYARCVEISTISA
                     QKEMVLPSSLAIAIPILMGLIFGVPGVMGLLIGGVATGFVLALFMANSGGAWDNAKKY
                     VEEGNFGGKGSEAHKAAITGDTVGDPFKDTSGPSLNILIKLMSMVAIVMAGLTAAFSL
                     L"
     gene            complement(15251..15964)
                     /locus_tag="PKN53_04315"
     CDS             complement(15251..15964)
                     /locus_tag="PKN53_04315"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOD88210.1"
                     /translation="MTNLSVNINKVATIRNARGGNIPDVKLAAIRCEMFGAHGITVHP
                     RPDERHIRYQDVYDIRPIIKTEFNIEGYPSKDFIDLVKKVKPHQVTLVPDPPDVLTSN
                     AGWDTVKHKDFLKEIISELKAEKIRTSIFVDTEFKNIEGAVLTGTDRVELYTEPYAAG
                     FEKNKEKAVEPFVKAGELALKLGLGLNAGHDLSLQNLKYFVQNVKGISEVSIGHALIS
                     DALYFGLDNTIKLYLRELA"
     gene            16055..16720
                     /locus_tag="PKN53_04320"
     CDS             16055..16720
                     /locus_tag="PKN53_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOD88211.1"
                     /translation="MIAKNLYSDTIPVLKTSNTGSEAIALMEMHRISHLPIVNNEEFL
                     GLISDSDIYDHNDLDAPIGTHNPSLIRPYVFENQHIYEIVDLFAKLKLTIVPVLDANK
                     KYLGSITLHNIVTEFAKMITVEKAGGIIILEMTIHDYCLSEIAQIVESNNAKILSLFV
                     NTIEASTAISITIKVDVTDMASIIQTFERYDYKIKASFLSEDMLKVFYQDRLDSFLNY
                     LNI"
     gene            16717..18099
                     /locus_tag="PKN53_04325"
     CDS             16717..18099
                     /locus_tag="PKN53_04325"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOD88212.1"
                     /translation="MKNYFIIFLLFALVLSKSAKNQNYIISEISINGNNKTKKEVILK
                     ELCFNEKDSIDYLTLEKAISESKQNLLRTSLFNYVDLNYKTDESQVFVNVNVEERWYW
                     WVYPIFEHGSRNLSSFIIDKDFSQINYGGVVELHNIKGKNNYFKIKVRGGYRQHYALT
                     LFLQNFEKTKNHGFKINAETFIQKKIISNILENKAVYSLSSFLNFNSHFVYSYRINLN
                     NQIGLGMAYKHYWVKDNNQETFISNYTSYYLNPIIFYNFDNRNNKVYPTNGFLININC
                     GYWYNLNNKFTSFIGNNFEFQLHKTIKNSRLAVHNISSYKFYKEDKNQNSFFQTQLEF
                     GKDFWIRGYEYFYFMGNNTVKTQNTLSFLLSKTKIHNLPKFLPNEFKKAYTKIYLEIF
                     GDLIYTDGYNKTFNLNNNFNKNINYTFGIGASFETYYDRLLQINLTYIPNISKIGIFV
                     NYKTPLIKLY"
     gene            18100..18981
                     /locus_tag="PKN53_04330"
     CDS             18100..18981
                     /locus_tag="PKN53_04330"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOD88213.1"
                     /translation="MNIAIFGRNFGNNFNGMAIQLFEELKNREVDFYINKKFFDFISE
                     KVLYTPLAKGVFQNSLPKDVKFDFVFSIGGDGTFLETASLVGDLGIPMLGINTGKLGF
                     LSYVSSNNLLTALNQIFEKEYSLEKRILIQIDADKEFYGDKNFCLNDFVIQKKSRLSM
                     IKTKVYADGNFINTYWSDGLIVSTPTGSTAYSMSCGGPIVAPAANVFVITPIANHNLT
                     VRPLVISADSEIEISVDSEEGKVLASLDHRAYGVDNFKKIVIKRANFHINFVNLFKNT
                     YFSTLREKLMWGTDIRN"
     gene            19028..19921
                     /locus_tag="PKN53_04335"
     CDS             19028..19921
                     /locus_tag="PKN53_04335"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOD88214.1"
                     /translation="MRKLFVFLLIVSFSGSFVSAQYNVHWRKIRHEVSVGGGTNNFLG
                     ELGGSDAVGSHFVKDFNFYASRWQFQVGYSYKLAEAWAVSTNLIYGRLYGSDELAGEE
                     NRYNRNISFRSPFIDLSANIQFSILKEKYGHRYDLKRVKGAKNTPNLYIFTGISGMYF
                     NPKGQYTDGKWYALQPLGTEGQGIIPTRDKYCRIALGIPLGIGVNYMLDRNLGIGAEY
                     CVKYYFTDYIDDVSSTYVDPAIFTDPMAAYFSSGTANPGWNGTGPGQQRGQTNYNDAL
                     MYLSVKLTYKLRPGAPGMPKF"
     gene            20021..20737
                     /locus_tag="PKN53_04340"
     CDS             20021..20737
                     /locus_tag="PKN53_04340"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOD88215.1"
                     /translation="MQQQTALLFFITILFFSPSKSIAQRGFEIGPTAGGSYYMGDVNM
                     YKHFYSTHLNLGLLMKYHINPRVGIRLSGIATKLSAADEDFKNPFQQERNHSFETNLI
                     EISSMVEITFLPYEIGDIKRKSFTPYIHTGLAAYLASSEQGLNMAVPIGMGIKKNIRP
                     RLVIGIEWTFKRTFSDGLDNLIGEDLKIYDPAYGTDITESNMNKQTGFLYNKDWYSFA
                     NLTISYTFKLGGLGCPAYYE"
     gene            20748..21488
                     /locus_tag="PKN53_04345"
     CDS             20748..21488
                     /locus_tag="PKN53_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOD88216.1"
                     /translation="MSLINKINKNNIPKHVAIIMDGNGRWAKARGKDRLSGHQAGANS
                     VKTSLEVCGELGIKFLTVYAFSTENWNRPKTEVLGLMNLLVGTIDKELDEIKKKKVKV
                     TVIGDMSRLDTLVQKKLKLAIEETKNNDLLNFIVALNYSGRWDIVNACKKLAQDCKEN
                     IIEPEKINENVFNSYLSTYNIPDPELLIRTSGEERISNFLLYQLAYSELYFTQTFWPD
                     FSKEEFYKAILSYQNRERRFGKISEQLT"
     gene            21490..24033
                     /gene="bamA"
                     /locus_tag="PKN53_04350"
     CDS             21490..24033
                     /gene="bamA"
                     /locus_tag="PKN53_04350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOD88217.1"
                     /translation="MMKKILQIIIFSCISLYLNAQHSSNQTVIDYSNPKEYEIGGVTI
                     SGIQYLDHNVLLYLTGLEIGNKITIPGDDITKAIKILWEQGLFSDVKISMTRQINDVV
                     FLDIYLEERPRLSKFSFTGVKKGEADDIRDLIKLVKGTQITENTILYSEKKIKDYFID
                     KGFRNVSVNTKQEIDTSLVNSKVLIFEIDKANRVKIENINFIGNENIGEEKKFYDFYL
                     SQEKRSDWKLRRAMKDTKQKTWYNIFKSSKMLPQQFEDDKKNIINKYNELGYRDARII
                     SDSIINNPDGTISVNIQIEEGKKYYFRNITWVGNTKYNSELLDRVLGIKKGDVYNHKL
                     LNERLLYDPAGVMSIYQDNSYLFSNINPVEILVENDSIDIEMRIYEGKQARINRVDIS
                     GNTRTNDHVIVREIRTRPGSLYKRSEVQRTIRELAQLGYFDPEKLNVDFQPDPVNGTV
                     DLEYLVEEKSTDQIELSGGYGGGIIVGTLGLTFNNFSLRNIFNAKAYHPLPSGDGQRL
                     ALRVQASGLWYQNYSFSFIEPWFGGKKPNSFTTSAYHSVMSNPSFQDTTTYYFKVTGV
                     GFGLGQRLRVPDDYFTLYNELSFKHYNLKDYPNPGFALYRTGRSNNISFKTILGRSSS
                     GPNPIFPTTGSNFSLSVELTPPYSLLNGKDYSANMTPQERYKWIEYHKYKFSGQWFTT
                     LAGSRDGSSRALVLMSKFEFGMLAMYNKEVGPSPFEAFQVGGDGLYGYNLYGLETIGL
                     RGYANNSLTPNNGGNIYDKFTLELRYPLSLAPTATLYMIGFIEAGNAWFASENFDPFD
                     LKRAAGVGVRFFMPMIGMLGVDWGYGFDELSGSAGANGSQFHFVIGQQF"
     gene            24097..24609
                     /locus_tag="PKN53_04355"
     CDS             24097..24609
                     /locus_tag="PKN53_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOD88218.1"
                     /translation="MKKIFVFIIALTCAGLAFSQKTAYVDTEYILNNIPTYESAKTQL
                     DEISEDWKNEIDQMKESIDKMYKDYQSERILLTEELRIKREEEIMKREKEMRDKQQKY
                     FGKDGLLFKKREELIKPIQDDIFNAIKEIAQEGSYSIIFDTANSLNMLYTDPKFDKSD
                     DVLKKLGYKN"
     gene            24644..25144
                     /locus_tag="PKN53_04360"
     CDS             24644..25144
                     /locus_tag="PKN53_04360"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOD88219.1"
                     /translation="MKKIIILFVVLGATLSSFAQKTKCGHVDTNSIFASMPERETATK
                     TVEEYAMTLETQLQDLSKELETKYTDYMANKETYSPSIAQMKEEEIVNLQQRIQAFQV
                     RAQEDLQNKEAELLEPIYTRIQEAIKTVGAEKSLLYVFEISTLLYFSEESVDITNDVK
                     AKLGIK"
     gene            25193..25990
                     /gene="murI"
                     /locus_tag="PKN53_04365"
     CDS             25193..25990
                     /gene="murI"
                     /locus_tag="PKN53_04365"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOD88220.1"
                     /translation="MNLPIGVLDSGIGGLTVLKALSTSLPNEDFIYYADSKNAPYGTK
                     TVDEIFILVDEIIKFLLDKKCKLIVIACNTATAAVVADMRKKYQIPIVGLEPAVKPAC
                     LNTKTGNIGVLATEGTFRGQLFIETSNKYKNYVNIHLQVAKGLVEAAENGIFEGEEIE
                     NILKKYINPFIEHNADYIVLGCTHYPLFAPIIKKLTNDKIQIIDSAEAVARRTKDLLI
                     TNSIFIKEPKKQNLKIYSTGDKTTITNVANKLLNVENNNTLVEYYFI"
BASE COUNT         9737 a         3902 c         3837 g         8636 t
ORIGIN      
        1 gtacgtgact gagtccgaat attgaaagca acgtggttgc tgaacgaagt tgaagcaaag
       61 cagtttgaca cttatgagac accgtctttt tataatatac tttcaatata atctaacaat
      121 tcttccttcc ctcttttagt tgttgaacta gtaataaaat aatcaggtaa attttcccaa
      181 tatttttcca aacttaattt ataatcttca atattctttt ttaatgcatt cactcctaac
      241 ttatcagttt ttgtaaaaac tatcttaaaa ggaacagagt ttaaacccaa ccaatacata
      301 tattctaaat ctattttctg agcagggatt cgactgtcca ccaagacaaa taagcaaaca
      361 agatttggac gttttaaaat ataatcttca agcatttttt taaatttctc tctcactgtt
      421 tttgaaactt tagcataacc aaatcccggt aagtcaacta aataccaaga tttatcgata
      481 ataaaatgat tgattaattg agttttacca ggcacagaag atgtttttgc caaatttgaa
      541 ttatttgtca acatattaat taatgaagac ttcccgacat tggatcttcc aataaaagca
      601 atttcaggaa tatcatcttg cggacaattt ctatagtcct gatttgacat caaaaattct
      661 gcagttttaa tttgcatttt taatttctat ataatatttt ttctaattcc ctaaataatc
      721 tttgttcatc ataaggttta gcaacaatat tcaaatcttt atcaactaaa tacattatcg
      781 gtgttgcata aacagaataa tcaattactt gttttccatc ccaagattta taatcacaag
      841 caatattcca agggaaagga tttttagata agaactcgtt taattctttt ttatctttat
      901 ctaaactaat agcaataata tcaacacttg ctgcatcaaa caattgttga gcttctgcaa
      961 aagtaggaat agtcaactga caatgtccac accatgttgc ccaaaaaaga attaaagtgt
     1021 aattcgactt catttttgac aaagtataag tttgcccact aagagtttga accgtaaagt
     1081 ctggagcttt cttaccaatt gtcaattctg tatttgcttt aattcttgct ttaagattat
     1141 cgcttgcatc actgcacaaa tctccaaact caagacttaa cttagaggat tgttcaacaa
     1201 gtccaattgt ttcaaaacca tttaaaataa attcaaaaat tgtattaaaa atcacaggct
     1261 cttcaaagaa aatatggtcg agaacatctt tacctgcaac ataatacaca tctgcatctg
     1321 ttgcacggaa cagttttaaa taatctaaaa ctactttttg ataagcatta gattttagca
     1381 ttaaaacact gctaaaatca taagatttta aatattcctt acgataaaat tcttgttttt
     1441 tctctatact aagcttttct ggtgcagtaa cttctttaaa atatgttaaa tatttgccgg
     1501 caaaactatt tttattcaaa ttaattactt taatcaacaa atcatttttc tctttaagct
     1561 gttttgcttt ttccttttct actgtttttg taaatgtttc gtcaggataa atagacataa
     1621 attgttctaa gatttgaatt ttatacttat acataaattc catttttaga aattcatata
     1681 attgaaaatt ctcattagac tcaataactt tcatattata aactggattt tcgacaaaag
     1741 tgcttaattg aatattttca ttattaaaaa tcaaatcaaa atactcatta ttgtcaagat
     1801 aaagtctata caaaccaact tcttttttat caaaagaaaa ctcaaaagca gaattgatat
     1861 tagtttgaac actttcgaca tattcagctt catcgccaaa taaagtaaaa actttcacct
     1921 ctctgtgagc ataattttca attctacatg ataatttata ttgagcaata gaataaatac
     1981 tcaaaaaaac agataaacat aaaaatgtta tttttttaaa agttaacata acttagcgat
     2041 attaaataaa aaagcgactg tctcaaaaca aattttaaaa caataaattt tcaaggtgtt
     2101 tcagaagtgt taaaatgcaa ggcgctgaaa ttgaggacga aggagcgtac cagaagcgtt
     2161 gcactgagca aagccgaagt gtacgtgact gagtccgaat attgaaagca acgaagcagt
     2221 ttgacactta tgagacaccg ccttttaact tattaataaa ttttactttt tacaagtctc
     2281 gcaatacaac tgtttaactc ttgtatttac aaaagtttgc acagatagtt tatcgccttt
     2341 aaacaaatcg cataaaaact caggatcttg ttttctgatt ctatctaatg cataaacaaa
     2401 attgtttgaa tgtactttat aatctacttt tgaacaaact gtagcataag ttgttaaata
     2461 cacaaatgga atgctttcat ctttaatcca aggttctagc aatcttatag catattcata
     2521 atcagagtga ttgataaata cactagctaa tttcaaagaa tttgcccatg ttaactcgtc
     2581 aatatgaata atctctttca ttttattcat agcttttaca acagatggat gatcatagcc
     2641 taatgaatct ttataaatat ccataatttg atattgcaac tcaatattca acaaatcaac
     2701 taaatcaaca cgtaaagaag tgttatacat tttgtcaata cgattttgta aagtttgttg
     2761 agtgcggtca ttatcaagat ctgttaaagt tactttacat aatacatcat taaaatcaac
     2821 ataaatattt gtttggtcta atttatttaa gtcatcaatt tttgttaaat aatcttcgtc
     2881 aagtacatcc atataaataa attgagttaa ccaaatttta ttcatattta atccaacata
     2941 ttctttgcct gaaggtattt tcatgtcggc aacaacattt tcgttatatc ttccttccac
     3001 aactcttttt agaatatatt tttgaattgc aagtgcagta tttatttgtc ctgcttcaac
     3061 agctctatta aattgtgtta caacatattt ttgttctttt tctccatcaa tatcataagt
     3121 aatccaaata gtaacatttg catatctatg gttttctaaa aacatttcca tttcctttgc
     3181 ctttgaattt acatatctaa ctgcttgttc aagatccata tcacaaacat cttcgaaaat
     3241 agttccttta cagtcgtttt gtaaatcttt taagtttggt gcagttatta cagaatccac
     3301 aatacttgca ttttgatttt cttctaaagc cttaacaata ctatttgctc ttctcttttg
     3361 taaagctgca ttttcagcta tagatccttc taaagaagaa tatgcttcaa taaagatttt
     3421 gttaataata aaatctggtt cattcaaagc tgctaacaca ggcttcatat cgtcaggttg
     3481 ataagtaaat tttccttgtt caaatggaat tctaaaattc aactctgttg aggttgctgt
     3541 tggtgcatat tcgcttattc ctgctggaag tatagtatcg ggcattaaag caattttagg
     3601 aacaaaatca taaatttttg tatcaaccca tgattgaggg atgttgtggc acacatgttt
     3661 ttctttaata accatcaaat tcaactcaat gttttgaggg tcaaaaccag caggtaattg
     3721 acctaaaaca acttctaatt ttgttacttt ttttcttctg ccctcgcctt cggctatatt
     3781 ctttttatat attttttttg aaaacatttt tttagtcatg aagccaatgt ttaccttaga
     3841 ataatcgaca atattttctg acttacaacg attaaattgg tctttttgaa caatatctac
     3901 tgctaagcca tctttcgaag ctctgataaa acgtctaaat tttcttaaat cattatattt
     3961 aaaaacaatt tcgccgttgt cattaatcga aagtccttct tgtaaatcaa caacatcggg
     4021 cattttcctt gttgttttct tacatatttt ttcgtcataa acttttaacc cctgtgactt
     4081 tacagaaatt ggagctgcca actctaacac attaccagca cctgagataa atgaagcata
     4141 attacccata tacacagaaa caaaaactct tttttgcgat gcgtcaactt taccactagc
     4201 tcctataaaa aagtattttt gtgacaacaa atcttttgaa tattttccag ttttccactt
     4261 atccaatgtt tgatttgcca attcgtcata actaatatat tcattagctg tccttatatt
     4321 agctcttgca accacttctg ctcctacgcc tgtaccgccg gcataagcca acctatctct
     4381 aactgttcca gcacgtccag aacctgtgag gtttgcttcg ccagtttctg ccatataagt
     4441 agcatgttcc ctagctggat ttataaaaaa atcatgaatc tcaaagcctt cgagtcctag
     4501 agaatccata tatgtattaa ttttataaac taatacatct tgaaacaatt tatcattaaa
     4561 attctctgga ttaattggat cactactcaa aatatcacct tccataaagg ttttttgagc
     4621 ttgaacatta acactaaaaa caagtgctaa acatacgata ctggtaaaaa tcttcctcat
     4681 ttaagtatta tatttagtta atattatttt tgcataaaaa taagtaataa tattcaataa
     4741 aaaaatattt ttgcatgaat aaaattcata aaatcaataa aaaaaacact aaattctcag
     4801 ctacaaagtt atacaaagtt ataaaatata tttttaggat tgtaaaaatt tacttttttt
     4861 attaactttt atttaacaac cccatatccc tatgaaaatc aacatattac aaacatcaga
     4921 taaaagtttg tgtttttcca tcaatctttt ttaatatctt tgtatttcaa ttaaatgttg
     4981 atagtgatgg aggaaaattt aagaatagat aaatttttgt gggcggttag attattcaaa
     5041 actcgtaatt tagctcaaga gaaatgcaat aaagggcatg tcttaatcaa tgataatgct
     5101 gttaagcctt caaaaaatgt taagattgga gatgttattt ttataaaaaa tccacctata
     5161 attagatcat acaaaatatt aaaactcact gataagcgag tatctgctaa actggttccc
     5221 gaccttattg ttgaaaccac tcctcaatca gaattagata aactaaaaaa tattgaaaca
     5281 gcatttatta tcagagacaa aggaagcgga agaccaacaa aaaaagaaag aagattaatt
     5341 aataaaatac aagaaaacaa cttatgataa ttgcagaaca aaaatataaa gagaatataa
     5401 ttgagtacat aatttatatc agacaaattc aagatattat aagacttgca aattgcgaca
     5461 ttgaagaaat aaacaaactt atcatagacc aatataaaac aagtgaaaaa ataaaaatta
     5521 aaatcagaga ctggtatttg gatttaataa atttaattaa aaccgagaaa attgataagc
     5581 aaggtgattt cggttttata aaaaacataa ttgacgaact tgaggaatta aatcaaaaat
     5641 tattaaacca agaaaactca tataaacacc aagaacttta caggtgggca aaacctaata
     5701 ttgaagagtt taaaaaactt tctaataacg caaacgaggg tgatgtaaaa acctgcattg
     5761 atgcacttca ttctttacta ttattaagaa taaaaaatca acctatttct agcgagacaa
     5821 tgcaagcaat gcaaactttt agtaatttaa tggcaaactt agcatttgag tataaaaaac
     5881 tcaaatcttg attatttata ttttgggtta tattttgaca aattcaaaat cttttgataa
     5941 tcaacctctt ccatcaattc ttcaagactt acatcttcat tcttcatata tgagcatact
     6001 attttaaaac ccaaccaatt gcctgttctt ggtggactat ctggaccaaa ttgatatgta
     6061 aaaggtgctg agccaacaaa ctttttaatt gttaaagcgt cagtagaaaa caacaattta
     6121 ttttcaacta ttgtagtcca catttcgcgt tcaaattttt cacaaaaatc taattgttcc
     6181 tttgtatatt tattaattct ttcaggttta aaatttggaa acatagaatg caagaaatac
     6241 aactttctac cattataaat catattttcc aataaatttt cggaatcgct ataaaaaggg
     6301 tattctgctt ctgcccaagc cgacaaaaca tcaattgcaa tttgctctct cgtcatttca
     6361 gattttgaaa aatcaggaat ttgcaacata tcgtacaatt cacaatcatt acctaaatat
     6421 ttatctaaac ctattccaat aaaatcttca gtgagcacaa ccgactgatt gaatccagca
     6481 ataaaagaaa caaagcgagg aattttaaca tcgggataat aatattttaa atgtttaaac
     6541 ccgtaagtca aatcttcatt cacatcatct agtttagaat attctttttc aacatatttt
     6601 cgtgcttgaa caacagaaaa atcatttaaa aatgtattta cataagacaa ataagccgaa
     6661 ttgtctgctc caccaattgc tattatttca taattatata cttcaaaaaa ctcttgatat
     6721 gttttttcca taaatgcaac ttgagtataa aaatctcctt tggaaaacct ttcaaaatct
     6781 ttatcaaatc gttctatttc aatgtttaac tcaatttttg aaatatcaac ttcaaactct
     6841 gctttctttt ttgaaaacac aaaaaacatt aaaattccaa ctaataaaac aaaaattaaa
     6901 attaaaacat aatatttctt cttcataata aactttatta aaaaaataat actatttttg
     6961 tagtacaaaa ttaaactaaa tttaaaatct taaaaatatg aaaaaaatta ttcttattct
     7021 aattagtttg tttttcttcg gaaacatttt tgctcaacaa gctatttctg gcggattata
     7081 tattggtcca acaatgtcgt ggatgagtac tgacagtaaa gtggcagata ctaaaggcat
     7141 gaaatttgga tacaattttg gagctttagt tgactttaac ttaattgata attttgcaat
     7201 ttctaccgga attcaattta acaatttagg aggtaatatc gcttacaaat atggatttac
     7261 taacttaaaa ccaaaagaag gtgctgttat agacactgta tttcctggaa ctaaaataaa
     7321 atataatctt aattacattg gagttccagt aagtttcaaa ggaaaaacta atgaaattgg
     7381 ctatatgaca tatttcttaa aagcgggagt tacaccatta attaatatta aaactttagg
     7441 ctcttttgga gaaaacgaaa aattaatgct aaaagaaata aatttattta atgttgcctg
     7501 gcatattgga ggcggaatag aatactcgct gtcaggaaat acaagaattt tagttgaaat
     7561 actttatact ggaggtttaa ttgatgttga caaaactaaa gttttcgatg tcccagatat
     7621 gacttctact agcaacccaa gaattgcatt aaatgatatt catttaaaag ttggtgtttt
     7681 attttaagca cataaaaaca aactttaaag caaaataatc aaactgtctt aaaagtgttc
     7741 aaatgtaatt cggcaattgg acgcttttaa gacagtgttt tttaacaaat cataaaaatg
     7801 aaaattgcaa ttgctcaatt aaactatcaa gttggaaatt tagattttaa ttctcaaaaa
     7861 attataaata caattttaac tgccaaaaat caaggagtag atttagtaat tttttcagaa
     7921 ctttcgattt gtggatacat accatatgat ttgttaaatt acaacttttt tattgatgat
     7981 tgctacaaaa aacttgaaga aataaaatta caatgcacaa acattgctgc aatagtaggt
     8041 ttaccgacaa aaaacctaaa aaatactggc aaaaaactat ataattctgc tgcttttctt
     8101 tatgatggag aaattcaaaa agtaattcat aaaactctga ttccaaattt tgacatcatc
     8161 aacgaagaca agtattttga agaaaatcaa gactttagca ttattaattt caaaaataaa
     8221 aaaatagctc taactattgc cgaagatttg tgggacgaag aaaaaattca cggctttttt
     8281 gaaaacaaaa taatgtttaa aaatgctcca atggaatatt tgaataagtt taatcctgac
     8341 tttatagtaa atattgctgc tcaaccattt gcctacaatc aaactgattg taaaaaagat
     8401 tttatttata aaaatgcaaa aaaatattct atacctatta ttaatataaa tcaagttgga
     8461 gcaaacactg aattaattta cgaaggtgct agcattgttg ttgacaaaac tggaaacaca
     8521 attatttctt ccaatagatt taaagaagat ttgaaaattt ttgagtttga cgaaattaca
     8581 aacacctcac aaacaaacaa tcacaataga aattcagatg acaaatacga atctatttat
     8641 tctgctctaa tacttggaat taaagatttt tttgaaaaat caaatcttaa aaaagctgta
     8701 gttggtttat ctggcggatt agattctgct gtggttgtag cacttttggt taatgctctc
     8761 ggaaaagaaa atgttgatgt tttattattg ccaacaaaat attcaagtca acattccata
     8821 gacgactcgc tagaaatggt taaaagatgt ggaataaaac atcatattgt tgatattgaa
     8881 gaacttagga aaatgtattt gaaaacaatg gaaaaagttt ttgaaaatac aaaagaagat
     8941 attaccgaag aaaatattca agcccgcatt agaggcagtg ttttgatggc ttattcaaat
     9001 aaatttggaa atattttgct caacacttca aataaaagtg aggcagcagt tggatattca
     9061 actatgtatg gcgacatgaa tggcgctctt gctgttattg gcgacttata caaaagcgaa
     9121 gtttacgaat tagcagatta tataaaccaa aatttcaata atatgattcc acaaaatata
     9181 attgacaaaa taccatcggc agaactaaga ctaaatcaaa aagatagcga ctctcttcct
     9241 gactatgaaa tattagacaa aattttattc ttatacatag aacaaaactt atctgaagaa
     9301 gaaattatta aaaaaggact tccacaagaa atagttcaca gaattattag attagtaaac
     9361 agtagcgaat ttaaaagatt tcaaatgcca cctgttttaa aaattagtcg aaaatccttt
     9421 ggcgatggaa gaaaaatgcc tttagtagga agattttaag aattattttt aattttgaaa
     9481 actaaaaaac agtaagatgg aagacagaac atttcaagaa atattagatt tttcaattct
     9541 agaattaggg agtgaattta atcttactgt attgaattta tttgagttag tattgttgtt
     9601 tatactcact ttcctcgctt tattcttaat taaaaaaact atttataaat caaagaaact
     9661 tgatttagcc aaaaaatatt ctctcaacaa tctcacaaaa tatgtagttt acaccatagc
     9721 ctttatcatt ttctttaaca ttctcggttt tgagttaaaa tatgtaatgg ctggttcggc
     9781 agctttattg gtcgtatagg tttaggttta caaaatttgt ttagcgattt tgtttctgga
     9841 attataatat tgattgattc ttccataaag gttggcgaca tacttgatgt agatggttta
     9901 atatgccaag tagaaaaaat aaatttgaga acaactttag tgcttacacg agacgacaaa
     9961 tttattcttt tgccaaactc attattgaca aaacaaaaaa ttgtaaactg gacacactca
    10021 aaaaatatat cacgttttga agttgatgtt agtgttgctc gttcttctga tgtagaacta
    10081 acaatgaagc ttataaagga agctggctta gaacaaaaag acgtattaaa agaccctgat
    10141 ccatttgtac gacttaataa ctttggaact tcctcactag actttacact ttatttctgg
    10201 gtaaacaatg tttttcgtgt ggaaaacaca aaaagtgcaa ttagagtaat cttatacaaa
    10261 aaattaaaat aaaacaatat caacattcca ttcccacaac acgtgcttca tgtagagaaa
    10321 gaaaatatag aggatgcaac caaggaataa aataaatcaa atgctaggct aaatttgata
    10381 gtacattaac ttttagaaaa gcacattaaa ttcgaacaac gagcaaagca taagtgtttt
    10441 ttgattttat tttattattt tatacgtttg caacgaatat ttaattttca tgaaatttaa
    10501 actaaaatta aaacactaca cgctatattt aggacttata gcctacatta taggaattgc
    10561 tttgaatatt ttttattctc tgtattcaaa tcaaccaggt ccgcaacagt cgcaatctcc
    10621 attgtttgaa attttgtctt tgttcccacc tataattatt ctagccttaa cattagtaag
    10681 tgcatcaatt tttgaagaat tggcatttcg aggatggact attaaacata agttttcaaa
    10741 atatatctca atcataggta ttttgtttta ttgtttttta gcaacaaaca gtttcataat
    10801 ttgcgtcatt gtattagctc tactaatttt tgtttttttc tacaaaaatg atgaattgag
    10861 ccaattcata aaagttattg ttacaagtct aatttttgca ttcatccact ttgataactt
    10921 tagcgacaat atgcgatata tcagcatctt gcaactattc ggagtatctc taattataag
    10981 ttactttgca cttagatttg gatttgttta cggaatatta atacatttcg caaataatct
    11041 tttagcaaca attataatat ttacaacatc tgccaattac agcacaagta ttgtaaaaga
    11101 aaattatatg gcaaatattg aaaaaatcga atttttagaa aattcatctt caatacaaga
    11161 aggtagtttt caagattcta ttgttcatgt cggaaatatt acacatattg ctgtagaatt
    11221 agctcctttt aataatgaag taatttacaa agcaaaatta agtagtttag actcatatca
    11281 ttatgtggct aaatgtaaaa acaaagaaac ttgcactctc gacaggcaag agcttttaaa
    11341 agacttttta aatcacacta atttaaaaac agatacaata ttagttgatg catttgtttt
    11401 aaaaattata gatattgata aattgcaaga gcatagagcc gatggacaca caacattgat
    11461 taagtatttg gtagaaggtt tgagaagcga accttttaat atacctattt ttatagatga
    11521 agacattgta gatattagtt ttattatgcc cgataatttt tatagagtat ttttgaaatt
    11581 atctactaaa gacaaaataa aaaaattgga aactgactat ggcattcttt tagaaaagac
    11641 aaatgataaa actgtttctg tggtttactt ttttgaataa aactcaaagt tttaatatcc
    11701 tttactcatt ctctaaaaaa tattgttttt caacatcata ttcagtaact tcatcaaaag
    11761 ggtctaaggc aacaatttta aacttgagta aatagctatt atcttcaaac actacagaat
    11821 ctgcaagcaa agaatcctta atttctgctt cgtgttcatc aaaaattaaa gtaataggag
    11881 caatttctct tccttgttgt cctttttgtg catcaacgga ttttggcaaa tattttgtta
    11941 aacatgcttt tgttgcaggc aaatcatcaa gtccacctat acttaaatca taattataaa
    12001 tagttgaaca agcaaaaaac gctaatatca aagaatacaa aattgctgga attattattt
    12061 ttttcattga gatataattt tagaagtaaa tttcgagtta tcatcaaaaa caaaattcac
    12121 tatatacaaa gattttgaaa ttaataaagg aattctaata aagttttttg gatttttttc
    12181 attaaaaact aagcgtccca tatagtcgta aacttgtatt tcactgactt ttttctgaga
    12241 ttcaaaattt aaaaaatcac ctacaatatt aaaagtaaaa tcattatttt tttcttcaag
    12301 ttttgaagaa ggtaagcaat aatttaaagt ttcgattata ttaaaataag atagagattc
    12361 tgaaactata taatcgcaaa ccatcattat ttgtggagta taattaattt catataatcc
    12421 aaacacatca gttgcatgtc ctgtagggaa gcatctaaaa gttagattat gatattcctt
    12481 aaaagcttct atttcttcgc tattataata atagtgtaca attccccaaa caattaaatc
    12541 ttgggttccc gaaccatatt gcctatgaat agaatccaca ataggagcta cttcaataca
    12601 agaaccacaa tttagagaga aaaaatgtag taaaaccact tttcctgccg caatgctatc
    12661 ataaagattt acttcctcgc cattagtatc taacaatgaa aaatcaggtg caggcttata
    12721 ggtttgcgcc agtaaaaact taaaatttat tataaaaaca aataatagaa ttaatatatg
    12781 cttcaatcta ttcatagaat cattatatta atataattaa acaaatataa atgttttttg
    12841 ttaaaaactt catacctatt aactaatatt ttataaattt taaaaataat atggtgattt
    12901 agttttgtat ttcaaaaaaa aataggaatt ttgcaaagtt ttttaaatgg ataataaaaa
    12961 tacataaaat atgattaaag aaaatatgtt ttggctagtt ccatttgctt ctattttggc
    13021 tctaggtttt gcttggtact tttttagaag catgatgaaa gaaaaagaag gaactcacat
    13081 gatgaaaaga gttgctttat atgttagaaa aggtgcaatg gcttacttaa agcaacaata
    13141 taaagttgta gcaattgttt ttattgcttt aacactttta tttgccatta tggcttatgt
    13201 cttaaaaatc caaaaccctt gggttccttt tgcctttatt acaggcggat ttttctcagg
    13261 tttagctgga tttttaggca tgagaactgc tacttatgct tcggcacgag ctgcaaatgc
    13321 tgcacgacac tcactaaatg cgagtttaaa acttgctttt aggtctggag cagtaatggg
    13381 tttagttgtt gttggcttag gtttattaga catttcgctt tggttttatg ttttacagta
    13441 tgcaattgga gcattagact atactaccaa tcctttaatt gctgccgatc catcaatgaa
    13501 gttgattata attaccacca ctaccctaac ttttggtatg ggagcttcga ctcaagcatt
    13561 atttgcacgt gttggcggtg gtatttacac aaaagctgct gacgttggag ctgacttagt
    13621 tggcaaatta gaagccgata ttccagaaga tgacccacga aacccagcaa caatagctga
    13681 taatgttgga gataatgttg gagacgttgc tggaatgggt gccgatttat acgaatccta
    13741 ttgcggttca atactttcaa ccgctgctct aggtgcagta gcattcaagt ctagcggact
    13801 tgacgttcaa tttaatgcag taattgcacc aatggtaatt gctgctgttg gaattatttt
    13861 atcaatacta ggaatattct tagtaaaaac caaagaaggt gcaaaccaaa aacaacttct
    13921 agctgcatta ggtagaggag ttaatgtaag ctcaatttta attgcttttt tcgcactagg
    13981 agtattatat tatttaggtc ttcctaatta tcaaggcatt tggattgcaa tgttggttgg
    14041 attattaggt ggaataggaa ttggaaaatc tacagaatat tttacttcat ctggatttaa
    14101 acctactcga aaaatctcag aatcggcaaa aacaggacat gcaacagtta ttattcaagg
    14161 tgttggaact ggtatgattt cgactgcaat tccagtttta attgtaacag catcaataat
    14221 tttatcttat ttatttgcag caaaattcga ctttagcaat attggaatgg gactttatgg
    14281 aattgcaata gccgcagttg gtatgctatc aactttaggt attacccttg caacagacgc
    14341 ttacggacct attgccgaca atgctggcgg aaatgctcaa atgagtggac tagaaccaga
    14401 agttaggcaa agaacagatg cattagacca actaggaaac accactgctg caacaggcaa
    14461 aggctttgct atcggctcgg ctgctttaac agctttagca cttttagctt catatatcga
    14521 agaaataaaa attgctatag gaagattacc cgaaaaagct agcgaattca tgagtactac
    14581 tggtgtagat atacattccg caactattcc agatattatg tcttttttcc aagtaaactt
    14641 aatgaatcct cttgttattg ttggaatatt tgttggagca atgacggctt tcttattttc
    14701 aggcttaaca atgaatgcgg ttggacgtgc tgcagaaggt atggttaacg aagtaagaag
    14761 acaatttaga gaaataaaag gaattttaac aggcgaagca gaaccagact atgcacgttg
    14821 cgtagaaata tcaacaataa gtgctcaaaa agaaatggtt ttaccttcat ctttagctat
    14881 tgcaatacct atacttatgg gacttatatt cggagtgcct ggagtaatgg gattgttaat
    14941 tggaggagta gcaacaggat ttgttttagc attatttatg gcaaattctg gaggtgcttg
    15001 ggacaatgca aaaaaatacg tagaagaagg aaattttgga ggcaaaggtt ccgaagctca
    15061 caaagctgca attactggtg atactgttgg agacccattt aaagacacat ctggaccaag
    15121 tttgaatata ttgataaaac taatgagcat ggtcgctatt gtaatggctg gactgaccgc
    15181 agcattttca cttttataat aaataaagtt tattgttaaa ttactaaaga attggcttag
    15241 gttgtaaaac ttaagccaat tctcttaaat atagtttaat tgtattatcc aaaccaaaat
    15301 aaagagcatc tgaaataaga gcatgtccaa tagaaacttc ggaaattcct tttacatttt
    15361 gaacaaaata ttttagattt tgtaaactta aatcatgtcc agcatttagt cctaaaccaa
    15421 gttttagagc taattcgcca gctttaacaa aaggctcaac ggctttttct ttattcttct
    15481 caaaaccagc tgcataaggt tctgtatata actctaccct atctgttcct gttaatacag
    15541 ctccttcaat atttttaaat tcagtatcta caaaaatcga agttctaatt ttttcagctt
    15601 taagttcaga aataatttct tttaaaaaat ctttatgttt aacagtatcc caaccagcat
    15661 ttgaagttaa aacatcaggt gggtcaggaa ctaatgttac ttgatgtggc tttacttttt
    15721 ttactaaatc aataaaatct ttcgatggat aaccttcaat attaaattct gttttaataa
    15781 taggacgaat atcataaaca tcttgatacc taatatgacg ctcatcagga cgaggatgaa
    15841 cagttatacc atgtgcgcca aacatttcac atcttattgc agcaagtttt acatcaggaa
    15901 tattaccacc acgtgcattt cttattgttg caactttatt aatatttaca cttaaatttg
    15961 tcatgtatta aaaaattaaa gtttttatta ttgtattttt ctgatacaaa tatattaatt
    16021 aagtttgcat aatcaaatat caaaaaacat atttatgatt gctaaaaatt tatattctga
    16081 tacaatacca gtattaaaaa catcaaatac tggcagtgag gcaatagcct taatggaaat
    16141 gcatagaata tcacatttac caatagtaaa taacgaagaa tttttaggat taatttccga
    16201 ctctgatatt tatgaccaca acgacttaga tgcaccaata ggaacacata atccttcact
    16261 tattagacca tatgtgtttg aaaatcaaca tatttacgaa attgttgatt tgtttgccaa
    16321 attaaaactt actattgttc ctgttttaga tgctaataaa aaatatttag gtagcattac
    16381 tttacacaat atagttactg aatttgccaa aatgattacc gtagaaaaag ctggtggcat
    16441 cataatttta gaaatgacta ttcacgatta ttgtttatct gaaattgctc aaattgtaga
    16501 atcaaacaat gcaaaaattc tttcattatt cgtaaacact atagaagcaa gtaccgcaat
    16561 aagtataaca ataaaagttg atgttacaga tatggcttca ataattcaaa cttttgaaag
    16621 atacgattat aagataaaag cttcgttttt aagcgaggat atgctcaaag ttttttacca
    16681 agatcgactt gattcttttt taaattacct aaacatttga aaaattattt cataatattt
    16741 ctgttatttg ctttagtatt atcaaaaagt gcaaaaaatc aaaattatat aatttcagaa
    16801 atttctatta atggaaataa caaaactaaa aaagaagtta ttctaaaaga attatgcttt
    16861 aacgaaaaag acagcattga ttacctaact ctcgaaaaag caatatcaga atcaaaacaa
    16921 aatcttttaa gaaccagtct gtttaactat gttgacctaa attataaaac tgatgaatct
    16981 caagtatttg ttaatgttaa tgttgaagaa agatggtatt ggtgggttta tcctattttt
    17041 gaacatggaa gcagaaattt atcaagtttt attattgaca aagactttag ccaaataaat
    17101 tatggaggag ttgtagaatt acacaacata aaagggaaaa ataattactt taaaataaaa
    17161 gttcgaggag gttacagaca acattatgcc ttaacactat ttttacaaaa ttttgaaaaa
    17221 accaaaaacc atggttttaa aataaatgca gaaacattta ttcaaaaaaa aattatttca
    17281 aatattcttg aaaacaaggc agtttacagt ttaagcagtt ttttgaattt caattcgcat
    17341 tttgtatatt cttacagaat aaacctaaat aaccagatag gtcttggaat ggcttataaa
    17401 cactactggg taaaagataa caatcaagaa acatttattt caaattatac ttcgtattat
    17461 ttgaacccaa ttatttttta caattttgat aatcgcaata ataaagtgta tccaacaaat
    17521 gggtttttaa taaacataaa ttgtggatac tggtacaatt tgaataataa atttacttcg
    17581 tttattggaa ataattttga gtttcaactt cataaaacaa taaaaaattc acgtttggcg
    17641 gtacacaaca ttagttcgta caagttttac aaagaggata aaaatcaaaa ttcatttttt
    17701 caaacccagt tagaatttgg caaagatttt tggattagag gatatgaata tttttatttt
    17761 atgggtaata atactgttaa aactcaaaac actttatcgt ttttattaag caaaaccaaa
    17821 atacataatt tgccaaaatt tttaccaaac gaattcaaaa aagcatatac taaaatatat
    17881 ttagaaatat ttggagattt aatttatact gatggatata acaaaacttt taacttaaat
    17941 aataatttta ataaaaacat taattataca tttggaattg gggcttcatt tgaaacttac
    18001 tacgacagac tattgcaaat aaatttaaca tatattccaa atatatcaaa aataggtata
    18061 tttgtaaatt ataaaacacc acttataaaa ttatattaaa tgaacatagc tatatttggt
    18121 cgaaattttg ggaacaactt taatggcatg gctattcagt tgtttgagga attgaaaaat
    18181 cgcgaagtag atttttatat aaacaaaaaa ttttttgatt ttatttcaga aaaagttctt
    18241 tacacaccac tagcaaaagg tgttttccaa aattctttac ctaaagatgt aaaatttgat
    18301 tttgttttta gtattggagg agacggcacc tttttggaaa ctgctagttt agttggagat
    18361 ttgggtatcc ctatgttagg tattaatact ggaaagttag gatttctttc ttacgttagt
    18421 tccaacaatt tgttaacagc actaaatcaa atttttgaaa aagaatacag cctcgaaaaa
    18481 cgaatactta tacaaataga tgctgataag gaattttatg gcgacaaaaa tttttgttta
    18541 aacgattttg taattcaaaa aaaatcgaga ctaagcatga taaaaacaaa agtatatgca
    18601 gacggaaact tcataaacac ctattggtct gatggattaa tcgtctccac accaacaggt
    18661 tctactgcat attctatgag ctgtggagga ccaattgtag ccccagcagc aaatgtattt
    18721 gtaataactc caatagccaa tcataattta actgttagac ctttggtaat ttctgctgat
    18781 tctgagatag aaatttcggt tgattcggaa gagggtaaag tattggcatc actagatcat
    18841 agagcatatg gagttgacaa ttttaaaaaa atagtaataa aacgtgcaaa ttttcatatt
    18901 aattttgtta atttatttaa aaatacttat tttagcacct tgagagaaaa attaatgtgg
    18961 gggacggaca taagaaatta acaccttgaa aatcagggtg tttgtgaaat tttaaaaata
    19021 ataaaagatg agaaaattat ttgttttttt gttaatagtt tcattttcgg gaagttttgt
    19081 atcggcgcaa tacaatgttc actggagaaa aatacgccac gaagtatctg ttgggggtgg
    19141 aactaataac tttttaggag aactaggagg ctccgatgct gtaggttcac actttgtgaa
    19201 agactttaat ttttatgcct caagatggca atttcaagtt ggttatagct acaaactagc
    19261 cgaagcttgg gcagtttcaa caaaccttat ttacggaaga ttatatggta gcgacgaatt
    19321 agctggcgaa gaaaaccgat acaacagaaa cataagtttt agatcaccat ttattgattt
    19381 atctgcaaat atccaattct ccatattgaa agaaaaatat ggacacagat acgatttgaa
    19441 aagggttaaa ggagcaaaaa atactccaaa tttatacata tttactggaa tttcaggaat
    19501 gtattttaat cctaaaggac aatatactga tggcaaatgg tatgccttac aacctcttgg
    19561 cacagaagga caaggaatta ttcctacacg cgacaaatac tgccgaattg ctttaggaat
    19621 tcctttagga ataggtgtaa attatatgct agatagaaat cttggtatag gggcagaata
    19681 ttgtgtgaaa tattacttta ccgattatat agatgatgta agtagcacat atgttgaccc
    19741 agcaattttc accgacccaa tggctgcata ttttagcagt ggaactgcaa atccaggttg
    19801 gaatggaact ggacctggtc aacaacgcgg acaaacaaac tacaatgatg ctttaatgta
    19861 tttatctgta aaacttactt ataaacttag accaggagca ccaggaatgc ctaaatttta
    19921 atatcacaca aaatatttta atttaatttt cgttgatagt aaaatgtggg gcagaaaaaa
    19981 catattatta attaatatag gtattacaaa aaaaacaata atgcagcaac aaactgcatt
    20041 attgtttttt attacaatat tatttttttc cccttcaaaa agtatagcac aaagaggatt
    20101 tgaaatcgga cctactgctg gaggctctta ctatatgggc gatgtaaata tgtacaaaca
    20161 tttttattct acacatttga atcttggact tttgatgaaa taccatataa atccacgtgt
    20221 tggaattaga ttaagtggta ttgctacaaa actttcagcg gcagacgaag attttaaaaa
    20281 tccgtttcaa caagaaagaa accacagctt cgaaaccaac ttaatagaaa tttcatctat
    20341 ggtggaaatt acttttttac cttatgaaat tggagatata aaaagaaaaa gtttcacacc
    20401 ttatattcat actggattag ctgcatattt agcatcgtct gaacaaggat taaatatggc
    20461 tgtaccaata ggaatgggaa tcaaaaaaaa tataagaccc agattagtta ttggtataga
    20521 gtggacattc aaacgaactt tttcagatgg tttagacaat ttaataggag aagatttaaa
    20581 aatttacgac cccgcatatg gaacagatat aacagaatca aacatgaaca aacaaactgg
    20641 ctttctgtac aataaagatt ggtattcatt tgccaaccta acaatatctt atacttttaa
    20701 attaggtgga ctaggttgtc cagcttatta cgaataaaat aaaataaatg tctttaataa
    20761 acaaaataaa taaaaataat atcccaaaac acgtagcaat aataatggac ggcaacggta
    20821 gatgggcaaa agctagaggc aaagatagac taagtggtca tcaagcagga gcaaattctg
    20881 taaaaactag cttagaagta tgtggcgaac ttggaataaa atttttaacc gtttatgctt
    20941 tttctacaga aaactggaat agaccaaaaa ccgaagtact gggactaatg aatcttttgg
    21001 ttggaacaat tgacaaagaa cttgacgaaa taaaaaagaa aaaagtaaaa gtaactgtaa
    21061 ttggcgatat gtcaagacta gacacattag ttcaaaaaaa acttaaacta gcaatagagg
    21121 aaacaaaaaa taatgactta ttaaatttta tagttgctct caattatagt ggaagatggg
    21181 acatagtaaa tgcatgcaaa aaattagctc aagattgtaa agagaacata atagaaccag
    21241 aaaagataaa tgaaaatgtt tttaactcat acttgtcaac ttataatatt cccgaccccg
    21301 aacttttaat aagaactagc ggtgaagaaa gaattagcaa ctttttactt tatcaattgg
    21361 cttattcaga attatacttt acccaaacat tttggcccga cttctcaaaa gaagaatttt
    21421 acaaggctat attaagttat cagaatagag aaagacgttt tggaaaaatt agtgaacaat
    21481 taacttaaaa tgatgaaaaa aattttacaa ataattatat tttcttgtat tagtctttat
    21541 ttaaacgctc aacacagttc aaaccagact gttattgatt attctaatcc taaagaatat
    21601 gaaattggtg gagttactat ttcaggaatt caatacttag accacaatgt tttattatat
    21661 cttacaggtc ttgaaattgg aaataaaata acaataccag gagatgatat aactaaagcg
    21721 attaaaattc tatgggaaca aggattgttt tccgatgtaa aaatcagcat gacaagacaa
    21781 attaacgatg ttgtattttt agatatttat ttagaagaaa gaccaagatt atcaaaattc
    21841 tcttttacag gtgttaaaaa gggagaagct gacgatatta gagatttaat aaagcttgtg
    21901 aaaggaactc aaataaccga aaatacaatt ttatattctg agaaaaaaat aaaagattat
    21961 tttattgaca aaggttttag aaatgtttct gtaaacacca aacaagaaat tgacacaagt
    22021 ttagttaact caaaagttct aatttttgaa atagacaaag ctaatagagt taaaatcgaa
    22081 aacattaatt ttatcggaaa tgaaaatatc ggagaagaaa aaaagtttta cgatttttat
    22141 ttatctcagg agaaacgtag cgactggaag cttcgcagag ctatgaaaga cactaaacaa
    22201 aaaacatggt ataatatttt taaatcttct aaaatgttgc ctcaacaatt tgaagatgac
    22261 aagaaaaaca taatcaataa atacaacgag ttaggttata gagatgcaag gataatatcc
    22321 gactctataa tcaacaatcc agatggaact ataagcgtaa atatacaaat tgaagaaggt
    22381 aaaaaatatt attttagaaa tattacttgg gttggtaata ctaaatataa ttccgaactt
    22441 ttagatagag tgcttggcat aaaaaaaggt gatgtttaca accacaaact tcttaacgaa
    22501 agattattgt acgatcctgc tggagttatg tcaatttacc aagataatag ttaccttttt
    22561 tcaaatataa atcctgttga aattttggta gaaaacgatt ctattgatat tgaaatgcga
    22621 atatacgaag gcaaacaagc tagaataaat agagtagata tttcaggcaa cacgagaact
    22681 aacgaccatg tcattgtgag agaaatcaga actcgaccag gatccctata taaacgttct
    22741 gaagttcaac gtacaattag agaattagca caacttggct atttcgaccc agaaaaatta
    22801 aatgtagatt ttcaaccaga cccagtaaat ggaacagttg atttagaata ccttgttgaa
    22861 gaaaaatcaa cagaccaaat agaactttcg ggtggatatg gtggtggaat tattgttggt
    22921 actttaggat taactttcaa caatttttcg ctacgaaaca tatttaacgc aaaagcatat
    22981 catcctctac caagcggaga cggacaaaga cttgcactta gagtacaagc atcaggactt
    23041 tggtatcaaa attattcatt ttcatttata gagccttggt ttggcggtaa aaagcctaat
    23101 tcattcacaa cttctgctta tcattctgta atgtctaatc ctagtttcca agatacaacc
    23161 acttattact ttaaagttac aggagttggt tttggtttag gacaaagact aagagtacct
    23221 gacgactatt ttactttata caacgaactt tcttttaaac attataattt aaaagattat
    23281 ccaaatcctg gttttgcact ttacagaaca ggtcgctcaa ataatattag ctttaaaacc
    23341 attcttggac gtagctcatc gggaccaaat ccaatatttc caactacagg ctcaaacttt
    23401 tcactctctg ttgagttaac accaccatat tccttactta atggaaaaga ctactctgca
    23461 aacatgaccc cacaagaaag atacaaatgg atagaatatc acaaatataa atttagtgga
    23521 caatggttta caactcttgc aggaagcaga gacggcagta gtcgtgcttt agttttaatg
    23581 tctaaatttg aatttggaat gttagccatg tataacaaag aagttggccc atcaccattt
    23641 gaagcattcc aagtgggtgg agatggactt tatggataca atttatatgg cttagaaaca
    23701 attggcttaa gaggatatgc aaacaactca ttaacaccaa acaatggtgg aaatatatat
    23761 gacaaattta cattagaact tagataccca ttgtcattag caccaactgc aaccttatat
    23821 atgattggat ttattgaagc tggaaatgca tggtttgctt cagaaaactt tgatccattt
    23881 gaccttaaac gtgctgcagg cgttggagtt agattcttta tgcctatgat tggaatgtta
    23941 ggagttgact ggggatatgg ctttgacgaa ttgtcaggct ctgcaggggc aaatggctca
    24001 cagtttcatt ttgtaattgg acaacaattt taaatttaat ttggaacaca ttttgcattt
    24061 atatattata ttagtaacat aaaaaatttg aaaattatga aaaaaatatt tgtttttatt
    24121 atcgctttaa catgtgcggg acttgcattt tcccaaaaaa ctgcttatgt ggatactgaa
    24181 tatattttaa acaatatccc aacttacgaa tctgcaaaaa cacaacttga tgaaatttct
    24241 gaagattgga aaaatgaaat tgaccaaatg aaagaaagta ttgataaaat gtataaagat
    24301 tatcaatccg aaagaatttt gttgacagaa gaacttagaa taaaacgaga agaagaaatt
    24361 atgaaaagag aaaaagaaat gagagacaaa caacaaaaat acttcggaaa agatggttta
    24421 ttgtttaaaa aacgtgaaga actaatcaag cctattcaag atgatatttt taatgcaatc
    24481 aaagaaattg ctcaagaagg ttcttattca attatttttg atacagctaa cagtttaaat
    24541 atgttataca cagatcctaa atttgacaaa agtgatgatg tattaaaaaa gttaggttat
    24601 aagaattaat tatttatatt tgccaaaatt ttaaaataaa gatatgaaaa agattatcat
    24661 tttatttgta gtattaggtg ctacattgtc aagttttgct caaaaaacaa aatgcggaca
    24721 cgttgataca aatagtattt ttgcgagcat gccagaaaga gaaactgcta caaaaactgt
    24781 agaagaatat gccatgactt tagaaactca attacaagat ttatctaaag aactagaaac
    24841 aaaatatacc gactacatgg caaataaaga aacctactca ccatcgatag ctcaaatgaa
    24901 agaagaagag atagttaatc ttcaacaaag aatacaagcg tttcaggtaa gagcacaaga
    24961 agatttgcaa aataaagaag cagaactttt agaacctata tacactagaa ttcaagaagc
    25021 tatcaaaact gtaggtgctg aaaaatcgct actctatgtt tttgaaattt ctacactttt
    25081 atatttttca gaagaaagtg ttgacatcac taacgatgta aaagcaaaac ttggaataaa
    25141 ataaaaaaaa attgtaattt taatcccgaa taaaatcggg atttttttta taatgaattt
    25201 acctatagga gtattagata gcggtatagg aggacttaca gttctaaaag cacttagtac
    25261 aagtttgccc aacgaagatt ttatttatta tgcagatagc aaaaatgcac cttacggcac
    25321 taaaacagtt gacgaaattt ttattttagt tgatgaaata ataaaattct tattagataa
    25381 aaagtgtaaa ctaattgtaa tagcatgtaa cacagccaca gcagctgttg ttgctgacat
    25441 gagaaaaaaa tatcaaatcc caattgtagg tttagaacct gctgtaaaac ctgcttgctt
    25501 aaatacaaaa actggtaata ttggagtttt agcaactgaa ggcactttta gaggacaact
    25561 ttttattgaa actagtaaca aatacaaaaa ctatgtaaat attcatttac aagtagccaa
    25621 aggattggtt gaagcagcgg aaaatggaat ttttgaaggt gaagaaattg agaatatctt
    25681 aaaaaaatac attaatccat ttatagaaca caacgccgat tatatagttt taggttgtac
    25741 ccactaccct cttttcgcac ctataattaa aaaattaaca aacgataaaa tacaaataat
    25801 agactcagcc gaagctgttg cacgaagaac taaagattta ttaataacaa attctatttt
    25861 cataaaagag ccaaaaaagc aaaacctaaa aatatatagc acaggcgata aaacaacaat
    25921 aacaaatgtt gccaacaaac tactcaatgt agaaaacaac aataccttag tagaatatta
    25981 ttttatttaa tttgtttttc ataacataga tgagtgtttt tacgtataaa agtacataaa
    26041 tcttgttatc aacttacaaa gttccttaaa tccgcaacta aaaataatta ttgcaatttt
    26101 atgtaaaatt at
//
LOCUS       DAOPNX010000026        25468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_2458_length_25468_cov_6.823240, whole genome shotgun sequence.
ACCESSION   DAOPNX010000026 DAOPNX010000000
VERSION     DAOPNX010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25468
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2458_length_25468_cov_6.823240"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PKN53_04370"
     CDS             <1..424
                     /locus_tag="PKN53_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88221.1"
                     /translation="VSMEGVERLNPEVEQYMILTKDEIYDGNYKASFINFQEETGCIN
                     RDSVGVELFTKDGYWRFFDEEFGAACNIGEYLDKKEGLVLVIELKNDSISRGKNYIGD
                     RWKILKLTNKELKIVNNVCIMTPCIFYDNNRTLTFEKE"
     gene            617..3628
                     /locus_tag="PKN53_04375"
     CDS             617..3628
                     /locus_tag="PKN53_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOD88222.1"
                     /translation="MKNYLAFIFLVFFVKFAFAQPVIEVQSPGLHKCVGESGELKITA
                     TGTGPLTYQWYHNGSPTGTNTNILSFASFVDSDDGDYYCEVSNSEGSVTSDIIVVRID
                     SNIPTINDVSTQFNIVCLGTNNNFSVDYTGTNCSFTWYHGGEVASYVQNYQLTNAVVG
                     DAGLYYCRVKNACGSVLSDNVEINVFGSPTITTQPHTTSVCEGGSVEFCVEAEGQYLN
                     YLWKKNGENLLAEQASCLQLANLSYPHSDVYSVYVYNTCGDITSNNVHATVNTVPTIT
                     GHPVDQIMCATDNVVLHGYGYSTTEVTYQWYNTDTGLIPNATDDNLTISISEGENTGY
                     YFVLSGTCGTVTSNTAEIIGKKAPIVLTQPIGAELCVGENHTLLIKTEGTLPMTYQWQ
                     KNSADVFGDNITGIQEATMIITGINAGQAGNYHCVVTNECGSITSDTVEVIVNTPPSI
                     IEFPQDMEFCEGQDFSLNVNTLGTEPLTIEWYVVGSETVVFEGSEFVFESALPEVSGD
                     YYCKVSNMCNTVYSDTISILVKALPEVIQNPIGAEICVGDSHEMSVTATGEQPLSYLW
                     YRNGSAISTETNSEIIISNAQINQTGIYFCRVANDCSYVDSEEVELVIGTPPAISWQP
                     INQNLCEHQTLNLIMDAQGENYSLQWYQNDVPISGANDTVLNIQNLSLENAGNYYCLA
                     YNACASVSTNIVEVVVKPSPEIDLGPDIELCDGETVTIGTDLPYSHYNWNNGLSHNQY
                     LEVALGGTFILEVIGSNDCPNSDTIVVTFHPYHSLASIPDNIVSCGPYTINAGDGAYS
                     YLWSNDATTSSITVNAPGTYSVTVTGDSFGCESTASIVVDLREPISFSLGNDVSAHVD
                     TYVNIGIEHIFSEYLWNTGFTGSMLTVYGSEYGNGTHEFWLTAYALNGCAHTDTIKVT
                     FYGQSGIEDEIANEISVYPNPASDFVIFESNDFLIENIEFYSVSGKLISKEIVNNWEV
                     KYNLSSFAKGFYLVKIVGENNKIINTKILVQ"
     gene            3632..4504
                     /locus_tag="PKN53_04380"
     CDS             3632..4504
                     /locus_tag="PKN53_04380"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOD88223.1"
                     /translation="MKEGKIKNKVRGAYLTSLVSISMVLLLLGIVGWMILGASFLSNY
                     VKENICFSLIIKNDVREADIKQYQKTLDAKNFIKATEYITKEDAANSLQEELGEDFIS
                     TLGYNPLSATLNVYLTADYAHPDSIAKIENFFLTESDIVEELAYQHNLLGLINDNINK
                     ISLVLLVFSAALLVISLALINNTVRLMIFSKRFIINSMRLVGAKRSFIRKPFLISGAL
                     QGFFSSLIAIAILSAIFYFVNKHIGEVVSLIDPLIIVSLYAILIIIGMLLTSISTYFS
                     VNKYLKLKTSNLYY"
     gene            4509..4769
                     /locus_tag="PKN53_04385"
     CDS             4509..4769
                     /locus_tag="PKN53_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HOD88224.1"
                     /translation="MNKIEKTENQEQNSGFTLGKKNYILIIIGFALIILGFILMSGGK
                     STDPNVFNEEIFNFRRISLAPIIILIGFIIEIFAILWRPKSK"
     gene            4779..5567
                     /locus_tag="PKN53_04390"
     CDS             4779..5567
                     /locus_tag="PKN53_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOD88225.1"
                     /translation="MEWFEALILGLIQGLTEFLPVSSSAHLEIGKALFGIKGEENFYF
                     SIMVHGATVLSTLVVFWKDILKLIKGFFKFKYNEETSYIFKIFVSMIPVLIVGLLFKE
                     HIEKLFAGNSFVFIGSMLIVTAALLFFTYFFKNKKQRDINYLDAFIIGISQAIAVVPG
                     ISRSGATIATGLINGNKKEEIAKFSFLMVLIPILGENFLDIVGGTAVSSGTPTSSLII
                     GFLAAFVSGLIACKTMINIVKKGKLYWFAIYCLIIGVITIFFVR"
     gene            5568..6305
                     /gene="truB"
                     /locus_tag="PKN53_04395"
     CDS             5568..6305
                     /gene="truB"
                     /locus_tag="PKN53_04395"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOD88226.1"
                     /translation="MIESFKLNTIPNFEQGEIIVIDKPLTWSSFDVVNKIRYKLREVT
                     GNQKIKVGHAGTLDPLATGVLVICTGKYTKLIEQLQEGEKVYEATFRLGATTPSYDME
                     TEVDKNFEIDNIKEEDIYKVAKSFLGTSMQTPPIYSAIQIRGKRAYEFARKGKKLKLE
                     QREINITKFDVSKINLPGVTLTIACSRGTYIRTLAHDFGKRLDNGAFLYYLRRIKSGD
                     YSIDDAIDLEGFIDFLDENKLTISCKK"
     gene            6327..7379
                     /gene="queA"
                     /locus_tag="PKN53_04400"
     CDS             6327..7379
                     /gene="queA"
                     /locus_tag="PKN53_04400"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOD88227.1"
                     /translation="MKLSQFDFNLPDELIALYPAENRDEARLMVLHRKTNEIEHKLFK
                     DIKDYFEEKDLMIFNDTKVFPARLYGTKEKTGAQIEVFLLRELNKEQRLWDVLVDPAR
                     KIRIGNKLYFGENDDLVAEIIDNTTSRGRTLRFLIDCDYPTFKEKIYNLGQTPLPTHI
                     IKREVEEEDKDRYQTIYAKNEGAVAAPSAGLHFSKILLKQLEIIGVEFGYVTLHVGLG
                     TFREVDVEDLTKHKMDSEQIFITEELANLVNKTKEEKHKVCAVGTTVLRTLESAVTTT
                     GYLKPFEGWTNKFLFPPADILVPDAMISNFHLPKSTLMISVAAFAGYHQLLKAYDVAI
                     KEKYKFGSYGDAMLII"
     gene            7383..7961
                     /locus_tag="PKN53_04405"
     CDS             7383..7961
                     /locus_tag="PKN53_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88228.1"
                     /translation="MKLLIRTGTLIFCILLIFIKIADAQHDNTSYDMLTSNEELNALL
                     TDEKLTDVKSRLFLTGVRNDTLFIVQYFSSENKIFLIPIAHLNDTSTLFCVRSGNYKT
                     SGNESCTISQLLETDGNTYKILQKMPCPQKEQITNEDKMQTVDVGEIGYLSRILKYTK
                     SKGVVDVEYLPFDKNLCTGCMKWFATEGKFVY"
     assembly_gap    8324..8423
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8424..9106)
                     /locus_tag="PKN53_04410"
     CDS             complement(<8424..9106)
                     /locus_tag="PKN53_04410"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOD88229.1"
                     /translation="MLEKVKINEVLFLDIETVPAQPNFESLEENFQKHWEKKSAYFRS
                     ENESASDVYNRAGIYSEFGKIICISVGFIYEKQLRVKSFYGDDEKQLLTEFANMISSW
                     DRFGNKQICGHNIKEFDVPYIARRMLVNGLNLPPAFDLAGKKPWEVNHIDTLELWKFG
                     DYKNFTSLALLADLFNIPTPKDDIDGSQVYAVYYEENNLERIVTYCEKDVLCVANLLL
                     RFMGKDIID"
     gene            complement(9109..9720)
                     /gene="udk"
                     /locus_tag="PKN53_04415"
     CDS             complement(9109..9720)
                     /gene="udk"
                     /locus_tag="PKN53_04415"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOD88230.1"
                     /translation="MLVVGIAGGTGSGKTTVVRKLIDQLPENCVTLIPQDSYYKDNSH
                     LPLEERQQINFDHPDAIEWSLLIEHIKELKQKKTIYQPIYSYLTCTRSEETIKVEPLK
                     IIIIEGILILGNKQLRSLMDLKVFVNADADDRLSRVIQRDIIERGRSVNMVLERYEKT
                     VKPSHLQFIEPTMRYADIIVPQGGNNDVAIDLLSKFILQNINK"
     gene            9788..10750
                     /locus_tag="PKN53_04420"
     CDS             9788..10750
                     /locus_tag="PKN53_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOD88231.1"
                     /translation="MLYPIKFKPIYKPKVWGGEKIKNLKSDKKTPDNCGESWEISALQ
                     GDLSVVANGFLKDNTIEEIIEVYMGEIVGDEVFENFGYEFPLLIKIIEAKENLSIQVH
                     PNDELAAERHNARGKSEVWYVLESEKGAKLISGFCKNTNSNEFFDAIEKEDFEYLLNI
                     VETKAGEVYYIPAGRVHSLGEGNLILEVQQTSDVTYRVYDYNRKDRELHLDLAKDVID
                     YSKTENLKINFSKKADNQNKIISNQFFTLNYIPIMNEIQKNYYNLDSFVVYFCVNGKV
                     QIKTPSNKTETLSKGETILIPAAIKDVNIIPEIYSEIIEIYIEV"
     gene            complement(10747..11664)
                     /gene="gldA"
                     /locus_tag="PKN53_04425"
     CDS             complement(10747..11664)
                     /gene="gldA"
                     /locus_tag="PKN53_04425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HOD88232.1"
                     /translation="MSIKVQNITKIFGTQKALDNVSFEIESGEIVGFLGPNGAGKSTM
                     MKIITGFIPPTNGEIFVNDKNIVEFQNYIKRIIGYLPEQNPLYLDMYVLEFLNFIAGI
                     YSLPNKKSRISEVVELTGLIPEMHKKIGALSKGYKQRVGLAQAIIHDPQVLILDEPTS
                     GLDPNQIIEIRNLIQDLGKSKTVLLSTHIMQEVEAICDRILIINKGIIVADGTTKTIQ
                     AKLKDKVKSLDLETLNPIDTKKLKLIAGVLAVRDLGKNHYNIEFDSKTDIRQDIFNLA
                     VENNNTVLSLQLKEHSLEQVFAELTTKAN"
     gene            11775..12182
                     /locus_tag="PKN53_04430"
     CDS             11775..12182
                     /locus_tag="PKN53_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88233.1"
                     /translation="MEQKDYILREIEKIGLILIAIKQKLFGGKENLAITIEKQMEETK
                     DALLTELKFDIDNFLSLDKAETEQYLSEFEGFNVENTEHLADIISQIGFAKKSENYLE
                     KALQLYEFCIFSSKTFSLDRERRMEEIRRCLGS"
     gene            12779..13129
                     /locus_tag="PKN53_04435"
     CDS             12779..13129
                     /locus_tag="PKN53_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88234.1"
                     /translation="MKKKLYIIFLIVIILCFITTGCVKQSNCDCCDEKIGRFVYYEEP
                     QEIIFCAEKTETSGLYITPEGHGVHIVGYIPKAFRVKDTIDVKICLKVIKREICRYYG
                     VPSVHKIKCIEKID"
     gene            complement(13384..15273)
                     /locus_tag="PKN53_04440"
     CDS             complement(13384..15273)
                     /locus_tag="PKN53_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOD88235.1"
                     /translation="MSDQIKHECGIALMRLRKPLSYYQEKYGTWRYGVNKLYLLMEKQ
                     HNRGQDGAGILGLKLNMRAGLKYMHRKRSVSSTPIIDCFNEIYRSFDIGIKSSPEKAN
                     DANWAKQNLDFVCDLYMGHLRYASYGKDNIDYVHPLLRVSNWRSKTLALAGNFNLTNV
                     DELFQYLIEFGQHPKECADTVTALELIGLFMEEENQKLFREYKNQGYKNYEITNLIEE
                     NIDIAKVLREATASWDGAYVMGGITGHGDSFLIRDPGGIRPAFYYYDDEIFVAASERP
                     AIQTALNITAEQVHELDPGMALIIKKNGTINTEMIKIPEKKTPCSFERIYFSRGTDHE
                     IYKERKKLGELLVPTILKAINYDFENTVFSFIPNTAETAFYGMLKGLETHLNEYKTKQ
                     IREKVDTMTDEEIRNLLELRPRVEKIAVKDVKMRTFITQDKDREEMVKHVYDITYKTI
                     NKKVDTLVIIDDSIVRGTTLRQSILKILDRLEPKKIIIVSSAPQVRYPDCYGIDMPRI
                     GDFVAFQAAIALAKENNKEDLIKEVYQLCKEQENLPKEDVVNYVRNIYKEFSAEQISQ
                     KIAQLIKNDSVNAEFQVIYQTIEDLHKACPNNHGDWYFTGNYPTPGGNKVVTHSFIDF
                     YEKAY"
     gene            15463..16389
                     /locus_tag="PKN53_04445"
     CDS             15463..16389
                     /locus_tag="PKN53_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOD88236.1"
                     /translation="MENLRDIEYFPEFDNLEFIASQVVEGFITGLHKSPFHGFSVEFA
                     EHRIYNKGESTKHIDWKLYARSEKLFIKRYEEETNLRGQIFLDISSSMLFPYENKIIN
                     KLNFSIYTAAALMYLLIRQRDAIGLSSFDDEIRFHANARSSKVHLQMIYNELRKYLNP
                     KNIGLQKKTNTAEVLHQLAEIINKRSLVIIFSDMFTSKDTDSEKLFAALQHLKYNKHE
                     VIIFHVTDKKFEQEFAFSNRPYKFVDLETGNILKLNPNEIRETYIERTQNLVQELTNR
                     SIQYGIDIIEADINKDFSEVLKPYLIKRAKFF"
     gene            complement(17159..18193)
                     /locus_tag="PKN53_04450"
     CDS             complement(17159..18193)
                     /locus_tag="PKN53_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOD88237.1"
                     /translation="MKKSFIKQIFIILVMSGIVFSCVPARQFEDVKSLKDKYQSERDE
                     LKKENERLATTNNELQAQLDKLKIDFNALATDTLIKGNAYRTLTVQYDKINELYSQLL
                     ANQEKLRQGADADAQKAMGLLQQTREELQRKEDELRNLEARLNQERANLDAMRIQNEL
                     KEKELADKNEKVQELQARLNAQDSIMNALRKKVSDALVGFEGDGLTVTQKDGKVYVSL
                     EEKLLFKSGRWDVDPKGVQALKNLASVLENNEEINILIEGHTDDLAYSGGGNIQDNWD
                     LSAKRATSIVKILLQNSNIDPTRLTAAGRGPYLPIDPAKTTEARAKNRRTEIILTPKL
                     DELYKLMNAN"
     gene            complement(18451..18813)
                     /locus_tag="PKN53_04455"
     CDS             complement(18451..18813)
                     /locus_tag="PKN53_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88238.1"
                     /translation="MKKIIFITILSVLFTIVLSSCTKEESEISGQIIYTGAISGIQYY
                     ASGAEVYLYYGSTTLCDDPIKTIADQNGNYSFTNLTDGNWQIYATVTVNGIKYQALTD
                     IIRTTGDDTQTVNLVLVN"
     gene            complement(19324..19989)
                     /locus_tag="PKN53_04460"
     CDS             complement(19324..19989)
                     /locus_tag="PKN53_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88239.1"
                     /translation="MNTIRCASKGIVFLALILLTACVKERTNYFSPENKDWITPYKIG
                     DSFVMKDNNNISHSFTLQSNNYYFTESAGGILFFTTRRANTEYHFQEFTSNYSAKFSL
                     SLTAAFYNDDEGDEIYIDLNDICFAYDLKNKTISRIYTPFGNLSNSTYEELDDNTQIK
                     STAEILDSYVVGNSNYSEVLHFEFKDFTEQWKDFTVKEIFVAKKYGLIKYVLNNNITF
                     ERE"
     gene            complement(19964..20566)
                     /locus_tag="PKN53_04465"
     CDS             complement(19964..20566)
                     /locus_tag="PKN53_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88240.1"
                     /translation="MLYKLQIFGFCLLSMLLLRACGRPPCETTVIDNGVLPESALEYV
                     PYISGKTYKFKHSNGLVVNFDASRETQKREEWSSCMRSCPYVIHYECNTTRLIPDYPI
                     FNFELNICNLDTLNYNCNVSLGKFGFHIPTGNFDTNYYEQVDSIKIDSVYYYQVFKLK
                     SYYDRYSSHDSIYADSMYYSYDKGIIKITLSNNEYYTLCE"
     gene            20683..23994
                     /locus_tag="PKN53_04470"
     CDS             20683..23994
                     /locus_tag="PKN53_04470"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOD88241.1"
                     /translation="MKFVKKPHNLIAVLTAVFSFIIYVLTLSPTVNFWDSGEFICCAN
                     TLQINHAPGNPTYLLLARIFSIFASSPQKIAFAINLFSAFSAAFAIFFVFKTIFWFSE
                     KLIAKKSNLDPKNSYLVSILASIIGALSLAFSSSFWAIATEAEVYSLSLFFTSLCLWL
                     MTKYLDEANQKANNLIVLIALMLGLSLGVHQLNILIIPALVFLFYFYKCKFSYKGFAI
                     AIVLAIGILIFVQIFVAGLPQILAWFEKIFTNHLSAGYYVGLISFSLIFILLIGLGIY
                     WSGVKNKKVLNLIFTSFLMFVIGYSSYAIIIIRSASNPPIDQNNVENIYNFVSYLNRE
                     QYGDRPLWRGQQFSSKLDKAKPYKKGEAIYDKTNGKYQLVHHKPVTNYKKSDIRYLPR
                     MWSDNPLHIYAYSEWTNITNSEIPRASQNFGFMFRYQFGHMYLRYFMWNFVGKQNNIQ
                     GHGDAIHGNWISGIKFIDNFRLGNQEKKPDFLKKNQTAYYFLPFILGLIGIFSQLKFD
                     KKNLFVISVFFIFTGIAIAFYLNQSPYQVRERDYAFLGSFYAFTIWIGLGITGLYLFL
                     HKFLKKKFLIFALGILGILISPAQMLLKNYKIQDKTNNDFAYHFARNLLNTCDQNAIL
                     FVSGDNETFPLWYLQECENFRTDVRVINLSFLNNDWYIDQIQRAERGNKAIKLNLDRR
                     KYISGKADILPIIDNTAKYYSFIYDKEYEKIDEEYSQIHDEFCSLLKKSGYANKYKLE
                     FESFEEYFRNIKAFGNNENFKDFCSIIYNLEDDEIQKYYGITEQQAKKLISDLDNFLT
                     SQILYLGNLESKLEFMFSEDTSLMIASKFYDYPTNYCPGYEFNMKLNKSKAIKYFEPI
                     GLREELLVDKIIWQIDTSQKYLNKSQLIAYQIINENNWERPIYFSSFLDKENYFGLES
                     YLYLEGLAYRLFPIKTEFTTNDLVNVNSYKMYDNFINKFKWGKLNYIDESIENILFLL
                     RADYTKLSRGLFLAQAYDAAEQVISHCIKVIPNKKVNFDYYTVGLVHSYYRLRKFPEA
                     SILTLMIAENVEKELEFYNSLSVELKSGLTQSYIKPKQTLEELMILAKQYEKSENTET
                     YKKLKQIYDKTINLK"
     gene            24006..24611
                     /locus_tag="PKN53_04475"
     CDS             24006..24611
                     /locus_tag="PKN53_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOD88242.1"
                     /translation="MSFLPIRYPKLFYCKALSRKKDRPKTLYLSFDDGPNPLVTEKVL
                     EILKKYKAKASFFCKGKNADLYPEIFNKVKAEGHTIGNHSYMHLDAFKHKNKKWLSDV
                     LRKSPVSEAYYFRPPYGHIFPWQYRVLRKKYKLVFWDVMVYDFRTDYSLKKIKKILFN
                     KVRDGSIIVFHDNIASSQKMLPALEFTLEYYTKRGYKFEKL"
     gene            24657..25418
                     /locus_tag="PKN53_04480"
     CDS             24657..25418
                     /locus_tag="PKN53_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005788098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOD88243.1"
                     /translation="MKIISFNLNGIRSAINKGFYDWLKNENPDILCVQETKAQESQIE
                     VDKFKELGYHSIIHSAVKPGYSGVAIFSKKQADFVQIGIGIDEFDYEGRLIRADFGDF
                     TQINSYFPSGTMGDVRQEVKYAYLDAVQNYVNELKKERPNIVLSGDYNICHKEIDISN
                     PKTKKGVSGFLPEERQWMTDFLESGFIDSFRVFDQSPEKYTWWSYRAGARKKNLGWRI
                     DYHMVSEPMRNKLISASIQNEVVMSDHCPIVVELG"
BASE COUNT         8983 a         3618 c         3999 g         8768 t
ORIGIN      
        1 agtttctatg gaaggagtag agagattaaa tcctgaagtt gaacaatata tgatattaac
       61 caaagatgaa atatatgatg gtaattataa agccagtttt ataaattttc aagaagaaac
      121 agggtgtata aatagagatt ctgtaggagt agaattattt acaaaagacg gttattggag
      181 attttttgat gaagaatttg gagctgcctg caatattggg gaatatttag ataagaaaga
      241 aggcttagtt cttgttattg agttaaaaaa cgatagcatt tcaagaggga aaaactatat
      301 aggagacaga tggaaaattt taaaattgac aaataaggaa ttaaaaatag tcaataatgt
      361 ttgcattatg acaccttgta tattctatga taataatcga acattaacat ttgagaaaga
      421 ataaactatt ggcgtttaaa cacaactgac agaaaacaaa aaaaatgata aaacaatagt
      481 attcaatttt attaataaat caactctttt tataaatagt aaattgttta taaatatttg
      541 ataaaatata tctattacat aatttatttt ttgtattttg cttgcttaaa ttgtttaaaa
      601 cataaagcca tatattatga aaaattattt agcatttatt tttctcgttt tttttgtaaa
      661 atttgctttt gctcaaccag ttatcgaggt gcaatcgcct ggtcttcaca aatgtgtagg
      721 tgaaagcgga gaattaaaaa ttacagcaac aggaacagga ccattaactt atcaatggta
      781 ccataatgga agtccaacag gaacaaatac taatatctta agttttgcta gtttcgtaga
      841 tagcgatgac ggggattatt attgtgaagt tagcaatagc gaaggtagtg ttacatctga
      901 tattattgtg gtaagaattg attctaacat accaacaata aacgatgtgt caactcaatt
      961 taatatagtg tgtttaggaa caaataataa tttctctgtc gattatacag gaacaaattg
     1021 ttcttttact tggtatcacg gaggagaggt tgctagctat gttcaaaatt atcaattaac
     1081 aaatgctgta gttggtgatg caggacttta ctattgtaga gttaaaaatg cttgtggttc
     1141 tgttttatca gataatgtag aaataaatgt ttttggttca ccaacaatta ctacacaacc
     1201 acacacaacc tcagtttgcg aaggaggctc tgttgagttt tgtgtcgaag cagaaggaca
     1261 atacttaaat tatttgtgga aaaaaaatgg agaaaatttg cttgctgaac aggcttcttg
     1321 tttacaactt gcaaacctaa gctatccaca ttcagatgtt tactcagtat atgtatataa
     1381 tacttgtggc gatataacta gtaacaacgt tcatgcaaca gttaatacag tgccaacaat
     1441 aactggacat ccggtagatc aaatcatgtg tgcaaccgac aatgttgtgc ttcacggtta
     1501 tggatatagt acaacagaag taacatatca gtggtataat acagatacag gattaatacc
     1561 aaatgcaacc gatgataatt taacaatttc tatatcagaa ggagaaaata ctgggtacta
     1621 ttttgtgcta tctggcactt gcggaacagt gactagcaac accgctgaaa ttataggtaa
     1681 aaaagcacct attgttttaa cacaacctat tggagcagaa ctttgtgtag gagaaaacca
     1741 cactctttta ataaaaacag aaggcacgct accaatgact taccaatggc aaaaaaatag
     1801 tgcagatgta tttggagata atattacagg aattcaagaa gcaacaatga ttattacagg
     1861 aataaatgca ggacaagctg gaaattatca ttgtgttgta actaacgaat gtggctctat
     1921 tacaagtgat acagttgaag taatagtaaa tacaccacca agtattattg agtttccaca
     1981 agatatggaa ttttgtgaag gacaagattt tagtttgaat gtaaatactt taggcacaga
     2041 accattaact atagaatggt atgttgtggg ctcggaaact gtagtttttg aaggaagtga
     2101 atttgttttt gaatcagctt taccagaagt atctggagat tattattgta aagtttcaaa
     2161 tatgtgcaat acagtttatt ccgacacaat tagtatttta gtaaaagcat tgccagaagt
     2221 aatacaaaat cctattggag cagaaatttg tgttggcgat tctcacgaaa tgtctgttac
     2281 tgcaacagga gaacaaccat tatcgtattt atggtataga aatggctcgg caatttcaac
     2341 agaaacaaat agcgaaataa taatttctaa cgcacaaata aatcaaacag gtatatattt
     2401 ttgcagagtt gctaacgatt gttcttatgt tgattccgaa gaagtcgagc ttgttattgg
     2461 aacacctcca gcaatttcat ggcagccaat aaatcaaaat ttatgcgaac accaaacttt
     2521 aaatctaata atggacgctc aaggcgaaaa ctactcttta caatggtatc aaaatgatgt
     2581 tcctatttca ggagctaatg atacagtttt aaatattcaa aatttaagtt tggaaaatgc
     2641 aggaaattat tattgcttag catacaatgc ttgcgcaagc gtatcaacca atattgttga
     2701 agttgttgtt aagccatctc cagaaataga tttaggacct gacattgaat tatgcgatgg
     2761 agaaacagtt actattggaa cagacttgcc atattcacac tataactgga acaacggttt
     2821 gagccataat cagtatttag aagtagctct gggaggaact tttattctcg aagtaatagg
     2881 aagtaatgat tgtccaaatt cagatactat agttgttact tttcatcctt atcatagttt
     2941 agcatcaata ccagacaata tagtatcttg tggtccatac acaataaatg caggagacgg
     3001 agcatattca tatctttgga gtaatgatgc aacaacatca agtattacag taaatgcacc
     3061 tggcacttat tctgtaacag ttactggcga tagctttggc tgcgaaagca ctgcttcaat
     3121 tgttgtggac ttacgtgaac ctattagctt tagtttagga aatgatgtct cagcacatgt
     3181 tgatacttat gtaaatattg gtattgaaca tatcttctca gaatatttat ggaatacagg
     3241 atttacagga tctatgctaa ctgtttatgg ctcagaatat ggaaatggaa cacatgaatt
     3301 ctggctaact gcctatgctt taaacggttg tgctcacaca gacacaataa aagttacatt
     3361 ttatggacaa tcaggaattg aagacgaaat tgcaaacgaa atatctgtat atccaaaccc
     3421 cgctagcgat tttgtaatat tcgaatcaaa cgatttctta atagaaaata tagagtttta
     3481 tagcgtttct ggaaaattaa tttctaaaga aattgttaat aattgggaag ttaaatataa
     3541 tcttagtagt tttgcgaaag gtttttattt ggtgaaaatt gtaggagaaa ataataaaat
     3601 catcaataca aaaatacttg tacaataagt aatgaaagaa ggaaaaatta aaaataaagt
     3661 aagaggagca tatctaacat ctttggttag tatatcaatg gtgttgcttc tacttggaat
     3721 tgtgggttgg atgattttag gtgcaagttt tttgtcaaat tacgttaagg aaaatatatg
     3781 cttttcatta attattaaaa atgatgtcag agaagcagat ataaaacaat atcaaaaaac
     3841 tttagatgct aaaaacttta ttaaagcaac tgaatatata accaaagagg acgctgcaaa
     3901 ctcacttcaa gaagaacttg gcgaagattt tattagcact ttgggatata atcctttgtc
     3961 ggcaacacta aatgtttact taacagctga ctatgctcac cctgatagta tagcaaaaat
     4021 agaaaacttt tttttgacag aaagtgatat tgttgaagaa ttggcatacc aacacaattt
     4081 attaggactt ataaatgata atataaataa aattagtctt gtattgttag tcttttctgc
     4141 agctttgctt gtaatttctt tggctctaat aaacaatact gtgagattaa tgattttctc
     4201 taaacgcttt attataaact caatgagatt agtaggagca aaacgaagtt ttatacgaaa
     4261 accattcctt atttcaggag cattacaagg attttttagc tcactaattg caatagctat
     4321 cttaagtgca atcttttatt ttgtaaacaa acatattgga gaagtagtaa gccttataga
     4381 cccactaatt attgtttcat tgtatgcgat actaattatt attggcatgt tgttgacaag
     4441 tatttcaact tatttctcag taaataaata tttaaaatta aaaacatcta atttatatta
     4501 ttaaaaatat gaataaaatt gaaaaaacag aaaatcaaga acaaaattct ggatttacct
     4561 taggtaagaa aaattatata cttattatta tcggttttgc cttaataata ttaggattta
     4621 tattgatgtc gggtggaaaa agcacagacc caaatgtgtt taatgaagaa atttttaatt
     4681 ttagaagaat ttcattagct ccaataatta ttcttattgg ttttattatt gaaatttttg
     4741 caattttgtg gagacccaaa tctaaataaa agaaatatat ggaatggttt gaagcattaa
     4801 tccttggatt aattcaagga ttaaccgagt ttttaccagt gagcagtagt gctcatttag
     4861 aaattggaaa agccttattt ggcataaaag gagaagaaaa tttttatttc tcaataatgg
     4921 tgcatggtgc aacagttcta agtactttag ttgtgttctg gaaagatatt ttaaaactaa
     4981 tcaaaggatt ttttaagttc aaatataatg aggaaacaag ctatattttt aaaatatttg
     5041 tttctatgat tccagtattg atagttggat tattattcaa agaacatata gaaaaattgt
     5101 ttgcaggaaa tagctttgtt tttattggct caatgctaat tgttactgcg gctttattgt
     5161 tttttacata ttttttcaaa aacaaaaaac aaagagatat aaattattta gacgctttta
     5221 ttatcggaat atcacaagca atagctgtag tgccaggcat ttctcgttca ggagcaacaa
     5281 tagcaacagg actaataaat ggaaataaaa aagaagaaat agcaaaattt tcattcttga
     5341 tggtgttaat tcctatttta ggagaaaact ttttagatat agttggaggc actgctgtct
     5401 caagtggaac acctactagt tcgttgatta tagggttttt ggcagctttt gtttcaggat
     5461 tgattgcttg taaaacaatg ataaatattg taaaaaaagg aaaactttat tggttcgcca
     5521 tatattgttt aataataggt gttataacta ttttcttcgt tagataagtg atagagagtt
     5581 ttaaactaaa tacaatccct aacttcgagc aaggagaaat tattgttatc gataaacctt
     5641 taacatggtc atcttttgat gttgtaaata aaattagata taaattacga gaagttacag
     5701 gaaatcaaaa aattaaggtt ggacatgctg gtactttaga ccctttggct acaggagttt
     5761 tggttatctg cacaggaaaa tacactaaac ttatcgagca attacaagaa ggagaaaaag
     5821 tttacgaagc aactttcaga cttggagcaa caacgccttc gtatgacatg gaaacagagg
     5881 ttgataaaaa ttttgaaata gataatataa aagaagaaga tatttataaa gttgcaaaaa
     5941 gttttttggg aacaagcatg caaacgccac caatatattc tgcaattcaa attagaggaa
     6001 aacgagctta cgaatttgct agaaaaggga aaaaactaaa actcgaacaa cgcgaaatta
     6061 atatcacaaa atttgatgta agtaaaatta atttgcctgg cgtaaccctt actattgctt
     6121 gctctagggg aacttatatt agaactttag cacatgattt tggaaaaaga cttgacaacg
     6181 gggctttttt gtattatctt aggcgaatta aaagtggaga ttattctata gacgatgcta
     6241 ttgatttaga ggggtttata gattttttag atgaaaataa attgacaatt agttgtaaaa
     6301 aataatttta ataacttatt taaaatatga aattatcaca atttgatttt aacctaccag
     6361 acgaattaat tgctttgtat ccagcagaaa atagagacga agcaagactt atggtattac
     6421 atagaaaaac taatgaaata gaacataaac tttttaaaga cattaaagat tattttgaag
     6481 aaaaagattt aatgattttt aatgatacta aagtttttcc agcacgcctt tacggaacta
     6541 aagaaaaaac aggtgctcaa atcgaagttt tcttacttcg cgaactaaac aaagagcaaa
     6601 gattgtggga cgttttggtt gacccagcgc gtaaaattag aataggaaat aaattatatt
     6661 ttggcgaaaa cgatgatttg gtggcagaaa ttattgataa tacaacttct agaggtagaa
     6721 ctttaagatt tttaatagac tgcgactatc caacttttaa agaaaaaatt tataatttag
     6781 gacaaacacc tctaccaact catataataa aaagagaggt agaagaagaa gacaaagata
     6841 gatatcaaac tatttatgca aaaaacgaag gagctgttgc agcaccttca gctggtttgc
     6901 actttagtaa aatactttta aaacaattag aaataatagg tgtagaattt ggttatgtta
     6961 cgctacatgt gggtttgggg acatttagag aagttgatgt ggaagattta acaaaacata
     7021 aaatggactc agagcaaatt tttattactg aagaattggc taacctagtg aacaaaacca
     7081 aagaagaaaa acataaagtt tgtgcagtag gaacaactgt tctaagaact cttgaaagtg
     7141 ctgttacaac tactggatac ttaaagccat ttgaaggttg gacaaataaa ttccttttcc
     7201 caccagcaga tattttagtg ccagacgcta tgatttcaaa tttccatctt ccaaaatcaa
     7261 ctctaatgat ttctgttgca gcatttgcag gctatcatca actattaaaa gcttatgatg
     7321 tggcaattaa agaaaaatat aaatttggat cttatggaga tgcaatgctg attatttaaa
     7381 atatgaaatt attaatcaga acgggaactt taattttttg cattttgcta atttttataa
     7441 agattgctga tgcacagcat gataacacat catacgatat gcttacgtct aacgaggagt
     7501 tgaatgcctt gttaacagat gaaaaactga cagatgttaa atcaagatta tttttaacag
     7561 gagtgagaaa cgacaccctt tttattgttc agtatttttc ttccgaaaat aaaatatttc
     7621 taattcctat tgcacatcta aatgacacaa gcacattgtt ttgtgtgcgc tctggcaact
     7681 ataagacttc aggtaatgag agctgtacaa tatctcaatt gttagaaaca gatggaaata
     7741 catacaagat tttgcaaaaa atgccctgtc ctcaaaaaga gcaaataaca aacgaagata
     7801 aaatgcaaac agttgatgtc ggtgaaatag gttatctctc aagaatatta aaatatacga
     7861 aatcaaaagg ggttgttgac gttgaatatt tgccttttga taaaaattta tgtactggct
     7921 gtatgaaatg gttcgccact gaaggaaaat tcgtttatta gttgagatga attttatttt
     7981 gcacgtttca ctaattccaa atcaaaaaat ctttcgactt tcaactttta acttttaact
     8041 ttcaactgat tatgcgtcag cctcttttgt tccttttgtg gaaaaaaact aaaaactctt
     8101 tcaacccaag cgagcagagt gagcttgcga accctgcgaa gcaagtacag acaaatcttg
     8161 cactaaagaa attctatcat tgtgaagttt ttaaaaactc actactaaaa actcaacact
     8221 caacactaaa aactctttca acttttaact gatttaacaa gatctttaaa atcaataata
     8281 tcttttccca taaaacgtaa caaaagattt gcaacacaca aaannnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnaaatcaa taatatcttt tcccataaaa cgtaacaaaa
     8461 gatttgcaac acacaaaacg tctttttcgc agtaggtaac aattctttct aagttgtttt
     8521 cttcgtaata aactgcataa acttggcttc cgtctatatc atcttttggt gtaggaatat
     8581 taaataaatc agccaaaagt gccaaagatg taaagttctt ataatctcca aatttccaca
     8641 actctaaggt gtcgatatga ttaacttccc aaggtttttt gccagctaaa tcaaaagcag
     8701 gaggtaagtt taaaccattt accaacattc tgcgagctat ataaggaaca tcaaattctt
     8761 taatattatg accacatatt tgtttgtttc caaatctatc ccaacttgaa atcatatttg
     8821 caaattctgt taacaactgc ttttcgtcat caccataaaa tgattttact ctaagttgtt
     8881 tctcataaat aaacccaaca gatatacata tgatttttcc aaattcagaa taaattccag
     8941 cacgattgta aacatctgaa gcagactcat tttcacttct aaaataagcc gatttctttt
     9001 cccaatgttt ttgaaaattc tcttctaagc tttcaaaatt aggctgtgca ggaacggtct
     9061 caatatctaa aaataaaact tcgttgattt taactttttc taacataatt acttatttat
     9121 attttgaaga ataaatttac ttaataaatc aattgcaaca tcgttgttac caccttgtgg
     9181 aacaataata tctgcatatc tcatagttgg ctcaataaat tgaagatgag aaggttttac
     9241 tgttttttcg tatctttcca aaaccatgtt gacagaacgt cctcgctcaa taatgtcgcg
     9301 ttgaataaca cgagataatc tgtcgtctgc atcggcattt acaaacactt ttaaatccat
     9361 taggctacgc aactgtttat ttcccaaaat taaaattcct tcaataataa taatttttaa
     9421 aggctcaact tttatagttt cctcactacg agtgcaagtc aaatacgaat aaattggctg
     9481 ataaattgtt ttcttctgct ttaactcctt gatatgttca atcaaaagcg accactctat
     9541 agcatcggga tggtcaaaat ttatttgttg tctttcttct aatggcaaat gactattatc
     9601 cttataatat gaatcttgag gaattaatgt aacacaattt tcgggaagtt ggtctataag
     9661 ttttctaact acagtagtct taccagaacc agttccgcct gcaataccaa ccactaacat
     9721 aatattatca tttttagaaa aaaaatatta attttgaatt tttaaaaata ataaaaatat
     9781 tttaaatatg ctatatccaa taaaatttaa accaatatat aagccaaaag tatggggtgg
     9841 cgaaaaaatt aaaaatttaa aatcagataa aaaaacaccc gataattgtg gcgaatcttg
     9901 ggaaatatct gcattacaag gcgatttgtc tgttgtggca aatggatttt taaaagataa
     9961 taccatagaa gaaattattg aagtttacat gggcgaaatt gtcggagatg aggtttttga
    10021 aaactttgga tatgaatttc ctttgctaat aaaaataatt gaagctaaag aaaatttgtc
    10081 aattcaagta caccctaatg atgagcttgc cgcagaaaga cataatgcca gaggtaaatc
    10141 agaagtttgg tatgttttag aatcagaaaa aggagcaaaa ttaatttcgg gcttttgtaa
    10201 aaacacaaat tcaaatgagt tttttgatgc aatagaaaaa gaagattttg aatatttact
    10261 aaatattgtt gaaacaaaag caggagaggt ttattatatt cccgcaggca gagtgcatag
    10321 cttgggcgaa ggaaatttaa ttttagaagt gcaacaaact tcagatgtta cttatagagt
    10381 ttatgattac aatagaaaag acagagaact gcatttagac ttggcaaaag atgtgataga
    10441 ttattcaaaa actgaaaatc taaaaattaa tttctctaaa aaagcggata accaaaataa
    10501 aataatttca aaccaatttt tcacattgaa ttacattcca attatgaatg aaattcagaa
    10561 aaactactat aatttagata gctttgtagt ttatttttgt gtaaatggta aagtccaaat
    10621 aaaaacacct tctaacaaaa cggaaacatt atcaaaagga gaaactattt taattcctgc
    10681 agcaataaaa gatgtaaata ttattccaga aatttattca gaaattattg aaatttatat
    10741 agaagtttag ttggctttgg ttgttaattc agcaaaaact tgttctaagc tatgttcttt
    10801 taattgaaga ctaagcacag tgttattatt ttcaacagca agattaaaaa tatcttgtct
    10861 tatatctgtt ttagagtcaa attcaatatt ataatgattt tttcctaaat cgcgaacagc
    10921 taaaacacca gctataagtt taagtttttt agtatcaata ggatttaatg tttctaaatc
    10981 caaacttttt actttgtctt tcaattttgc ctgaattgtt ttagtagtgc cgtcggcaac
    11041 gataatacct ttatttataa ttaaaattct atcacaaata gcttcaactt cttgcataat
    11101 gtgagttgat agtaaaacag ttttgctttt acctaaatct tgaattaagt ttctaatttc
    11161 aataatttga tttgggtcta agccagaagt gggttcgtct aaaattagta cttgcgggtc
    11221 gtgaattata gcttgagcca aaccaactct ttgcttataa ccttttgata aagctccaat
    11281 ttttttgtgc atttcgggta tcaagcctgt aagttctaca acttcactaa ttcttgattt
    11341 tttgttaggt agagaataaa tcccagctat aaaattcaga aactcaagca catacatatc
    11401 caaataaaga ggattttgct caggcagata gccaataatt cgttttatat agttttgaaa
    11461 ttcaacaata tttttgtcat taacaaaaat ttcaccatta gtaggtggaa taaaaccagt
    11521 tattattttc atcattgttg attttccagc accattggga cctaaaaaac caacaatttc
    11581 gccactttca atttcaaaac ttacattatc taatgctttt tgtgtgccaa aaatttttgt
    11641 aatattttgt actttaatcg acattgaaat taaattttaa gaatgtgtaa aattagtttt
    11701 ttattttaat tattaaaaac taaaaccact ttataaataa aaatggttta tctttacgtt
    11761 gaaattttag caaaatggaa caaaaagact atatacttag ggaaatagaa aagattggac
    11821 taatactaat tgcaataaaa caaaaacttt ttggtggaaa agaaaatttg gcaattacta
    11881 ttgaaaaaca aatggaagaa actaaagatg ctttattaac agaactaaag ttcgatatag
    11941 ataattttct tagtttggat aaagctgaga ccgaacaata tctttctgag tttgagggat
    12001 ttaatgtgga aaacacagaa catttggcag atattatttc ccaaataggt tttgctaaaa
    12061 aatcagaaaa ctatttagaa aaagcacttc aactttacga attttgcatt tttagtagta
    12121 aaacattttc gttagataga gaaagaagaa tggaggaaat tagaaggtgt ttggggagtt
    12181 gaaatagtca aatgtattat aattttcatt tttagatatt tttcaaaatg aagttttata
    12241 ttttattacc aaaacagtac aaattttaac ccctggactt ttttaaaatt ttaaaataaa
    12301 tacttacatt tttcttgatt ttctaaaaat aatattgtat ttttgagaaa tgtttagttg
    12361 atgaggtttg ttgtaaaaac gaaaaatgta tgtgcacatg taaatattgt aaattttatt
    12421 ttactaatgt ttctatgtac aaaactattg cattgtaata aattatattt acttttaaat
    12481 tcaattaaga agatcgttta cattgtagta gatagctgta tttttttata cgacaaaatt
    12541 ataaatattg cagtatttaa ggagatacac acatatatct aataacagca ttccagcaat
    12601 atatatactt atttcaattt ggaagctatg taaaaacgat tttgattgtt tgccctcgtt
    12661 taaatggtgg ttgcagtatt agcaacctaa gcgagggtat aaataaagct gtttttggtc
    12721 ggaatatgcc gaaagagact aaaaaccgaa gtaggcacaa tttataaaac ttaaaattat
    12781 gaaaaaaaaa ttatatatta tatttttaat tgtaattata ttatgtttta ttacaacagg
    12841 ttgtgttaaa cagagtaatt gcgattgttg tgacgaaaag ataggtagat ttgtgtatta
    12901 tgaagaacca caagaaatta ttttttgtgc tgaaaaaaca gaaactagcg gtttatatat
    12961 aaccccagaa ggtcatggag ttcatatagt tggttatatc cctaaagctt ttagagtaaa
    13021 agatacaatt gatgtaaaaa tttgcctcaa agtaataaag agagaaattt gtcgttacta
    13081 tggtgtacct agtgtgcata aaattaagtg tatagaaaag atagactgat tttaaatgtt
    13141 ttaaataggt caattggata caaaacgaac tgtttggtat aaacttctaa aaatagtaaa
    13201 gagccaataa gtcagcaatg agttaattgg ctcttttgca tttttactaa taaaacattt
    13261 tcgttagata gagaaagaaa aatggaggaa attagaaggt gtttggggag ttgaaaatac
    13321 taaaatctca ctttaaaaac tttacaaact ttacgaactt taaaacttta tgaactttac
    13381 aaactaatat gctttttcat aaaagtctat aaatgaatgt gttacaacct tgtttccgcc
    13441 gggagttgga taattaccag tgaaatacca atcgccatga ttgttaggac aagctttatg
    13501 taaatcttca attgtttggt atatcacttg aaattctgca ttaacagagt cgttttttat
    13561 tagctgggca attttttgag atatttgttc tgcagaaaat tctttgtaaa tatttcttac
    13621 ataatttaca acgtcttctt ttggtaaatt ttcttgctct ttacaaagtt ggtaaacttc
    13681 tttaattaaa tcctccttat tgttttcttt tgctaaagca atagccgcct gaaaagcgac
    13741 aaaatcacca atcctaggca tgtctattcc ataacaatcg ggatatctga cttgaggagc
    13801 agaagaaaca attattattt ttttaggctc taatctatct aaaattttta aaatactttg
    13861 tctcaaagtt gttcctctaa caatactgtc gtcgattatt acaagtgtat caactttttt
    13921 attaatagtt ttataggtaa tgtcgtaaac gtgttttacc atttcttctc tatctttatc
    13981 ttgagtgata aaagtcctca ttttcacgtc ttttacagca attttttcaa ctcttggacg
    14041 aagttctaac aagtttctga tttcttcatc agtcattgtg tcaacttttt cacgaatttg
    14101 cttggtttta tattcgttta aatgagtttc caagcccttg agcataccat aaaaagctgt
    14161 ttcggcagtg ttaggaataa aagaaaaaac agtattttca aaatcgtaat ttattgcttt
    14221 taagattgtt ggaacaagca gttcgccaag tttttttctt tctttataaa tttcgtggtc
    14281 tgttcctctt gaaaaataaa ttctctcaaa actgcaagga gtttttttct caggaatttt
    14341 aatcatttca gtgttaattg ttccattttt ctttatgatt aatgccattc ctggatctaa
    14401 ttcatggact tgttcagcgg taatgttcaa agctgtttga atagctggac gttctgaggc
    14461 tgcaacaaaa atttcatcat cataataata aaatgcaggt ctaattcctc ctggatctct
    14521 aatcagaaaa ctatcgccat gaccagtaat accacccatt acataagcgc cgtcccaact
    14581 tgctgtagct tctcttaaaa ctttagcaat atcaatattt tcttcaatta gatttgtgat
    14641 ttcatagttt ttatagcctt gatttttata ttcacggaaa agtttttgat tttcttcttc
    14701 cataaataaa cctatcaact ccaaagctgt aacagtatcg gcacattctt taggatgctg
    14761 tccaaattca attaaatatt gaaataattc atctacatta gtaagattaa aattgcctgc
    14821 taaagctaaa gttttgcttc gccaattact cactctcaac aaagggtgaa cataatcaat
    14881 attgtctttt ccatagcttg catagcgtaa atgacccata tataaatcgc aaacaaaatc
    14941 taaattttgt tttgcccagt ttgcatcatt agctttttcg ggagagcttt ttataccaat
    15001 atcaaaactt ctataaattt cattaaagca atcaattata ggtgttgaac taaccgaacg
    15061 ctttctgtgc atgtacttta agcctgctcg catgtttaat tttaaaccta aaatcccagc
    15121 tccatcttga ccacgattat gttgtttttc cattaataaa tataatttat taacaccata
    15181 acgccaagtt ccatattttt cttgataata cgataaaggt tttcttaaac gcattaaagc
    15241 aataccacat tcatgtttga tttgatcgct cataaaaact gcctaatatt gaaatagatg
    15301 aatttgttta aacattggac aaaattaata tttttaaaca agttgtaaaa aactttaaga
    15361 ttttcttaac attaaaatat caacatataa tttgtcaaaa caaaatatgt ggcttaaaat
    15421 gaattaacaa ttgtattttc gtatatttgc aaaaattatt gaatggaaaa cctaagagat
    15481 atagaatatt tcccagagtt tgataatctt gagtttatcg cttctcaagt ggttgagggg
    15541 tttattacag gtttacacaa aagtcctttc catggctttt cggttgaatt tgccgaacat
    15601 cgtatttaca acaaaggcga aagtaccaag catattgatt ggaaacttta tgctcgtagc
    15661 gaaaaacttt ttataaaacg ttatgaagaa gaaacaaact tgcgtggtca gatattttta
    15721 gatatttctt catcaatgct tttcccttac gaaaataaaa ttataaataa gcttaatttt
    15781 tcaatttaca ccgcagcagc tttaatgtat ttgcttattc gtcaacgcga tgctattggt
    15841 ctttcaagct ttgatgacga aattagattt catgcaaatg ctcgttcttc gaaagttcat
    15901 ttacaaatga tttataatga gcttagaaaa tatcttaatc caaaaaatat aggactacag
    15961 aaaaagacaa ataccgcaga agtgcttcat caacttgctg aaataataaa caaacgctct
    16021 ttagttatta tttttagcga tatgttcaca agtaaagaca cagattctga aaaacttttt
    16081 gcagcacttc aacacttaaa atacaacaaa cacgaagtga taattttcca tgttactgat
    16141 aaaaaatttg agcaagaatt tgctttttcg aacagacctt acaaatttgt tgatttagaa
    16201 acaggaaata ttttaaaact taacccaaac gaaattcgag aaacttatat agaaagaact
    16261 caaaatttag tgcaagaact tacaaaccgt tctattcaat atggaattga tataatcgaa
    16321 gctgatataa acaaagactt tagcgaagtt ttgaaaccat atttaattaa gcgtgccaaa
    16381 ttcttttagg cggtttcggt ggcttataag tattcaactg ctttacttca acttcgttca
    16441 gcaaccacgt tgctttcgat attcaaactc agtcacgtac acttcggctt tgctcagtgc
    16501 atcgcttctg gtacgctcct tcgttttcaa tctcagacgc cttgcatttg aacacttcta
    16561 agccaccttg gtttagtggt ttttattaag ctatgtatta ttttgtcctg aacaatattt
    16621 gataaaggcg gtggcttata agtgtcctga cttgagttta taaagttcgt aaagaaggta
    16681 agtgtttgac tgtcaaaagt ttgcccgaag ggcaacatgt gattaaccgt aggcgcagcc
    16741 tgcggtatga tctcctcaaa agccttgccc gcaagggcaa cacaatcaaa tcagttaaaa
    16801 gttaaaaagt tttaaagttc gtaaagttaa atccgtttga gcaatagact aaacactaaa
    16861 aactctttca actttcgact tttaactgat gtgcatttga acacttctaa gccaccttgg
    16921 ttgtttgatt aggataattt cacaacattt gctcgctcgg atttgcaatc cgagtgtttt
    16981 cacaattaaa tatttttccg cacggattgc aaatccgcgc gagcaaggat gaaaaataga
    17041 tttgcattgg taacaaaaga cgacatacac cgtccctaca ctatgctata tagttctttc
    17101 aatcagactt taaaaacttt atgaacttta caaactttat gaacttttaa ctgatttgtt
    17161 agttcgcatt catcagttta taaagctcat ctaacttagg tgtaagaata atttctgtac
    17221 ggcgattttt tgctctagct tcggttgtct tagctgggtc tataggtaaa taaggacctc
    17281 ttcctgcagc tgtcaatctt gttgggtcga tatttgagtt ttgtaaaaga attttcacaa
    17341 tagaagttgc tctttttgca cttaaatccc agttgtcttg aatgttccca ccacctgaat
    17401 atgctaaatc atcggtatgt ccttcaatca aaatatttat ttcttcatta ttttctagaa
    17461 ctgaagcaag attttttaga gcttgcacac cttttggatc aacatcccat cttccagatt
    17521 tgaataataa tttttcttcg agtgaaacat aaacttttcc atctttttgt gtaactgtta
    17581 gtccatcgcc ttcaaaacct actagtgcgt ctgaaacttt tttacgcaaa gcattcatta
    17641 tagaatcttg agcatttaaa cgagcttgta attcttgaac tttttcattt ttatcggcta
    17701 attctttttc tttaagttcg ttttggattc tcatagcatc aagatttgct ctttcttggt
    17761 ttaatcttgc ttctaaatta cgcaattcat cttcttttct ttgtaattct tctcgtgttt
    17821 gttgtaaaag acccatagct ttttgtgcgt cagcatctgc accttgtcta agtttttctt
    17881 gatttgctaa caattgagaa tataattcat taattttgtc atattgtaca gtcaatgttc
    17941 tataggcatt acctttaatt aaagtgtcgg tcgctaaagc gttaaaatca atttttagct
    18001 tatctaattg tgcttgcaac tcgttgtttg ttgttgctaa tctttcattt tcttttttaa
    18061 gctcatctct ttcgctttga tatttgtctt tcagagattt aacatcttca aactgacgtg
    18121 caggtacaca agaaaatact atgcccgaca tcacaagaat tataaatatt tgtttaataa
    18181 atgatttttt cattgcaata aaatttaata ttcatacaaa aataatggtt ttaattcaaa
    18241 aaatatagga taaaaatatt ttttatttac aggtgtttgt tagttaaatt tttaggagat
    18301 taatgcttgc aaaaaagtga tgggaaatca agtttgctat tatttcttag ttttaaattg
    18361 tattttttgt tttgcttagt agaatggaat aaactcattg aactgattgt tagtcaacag
    18421 aacaatgagt ttatccaagc aaattgtcat ttaattaaca agaactaagt tgacagtttg
    18481 agtatcgtct ccagttgtgc gaataatatc agtaagggct tgatatttga tgccgtttac
    18541 tgtaactgtg gcataaattt gccaatttcc gtcggttaaa tttgtgaaac tataatttcc
    18601 attttggtcg gcaattgttt taattggatc atcacataaa gttgtggagc cataatacaa
    18661 atatacttct gcacctgaag catagtactg aattccactt atagctccag tataaataat
    18721 ttgaccagaa atttcacttt cttcttttgt acaactgctt aatacaatag taaataaaac
    18781 acttaaaatt gttataaata taattttttt catgttataa ttcttttaat ttatttttcc
    18841 tactcatatg gcttttcggt tacgcctcgt tttttagagt ggtttctgat ttccactttt
    18901 taaattatta agattgtttg aagatttggc aacatttagg cggttctctt gtctttaaca
    18961 ttttagttac tttttgtgcc tttaagttcc aattgggcgg tttataaaaa aaagcgtgga
    19021 actgtaactt tatttgcttt cgaggtaccg ccagacacct acacatcaaa taagtacatc
    19081 ccacgccgta gcgtgagcat ttgaacttgt tatttctgat gtgtttctga aaattggcgg
    19141 ttttcgaaag caaaaataaa agttaacgct tcgttatttt actattttat aattattaag
    19201 tcattatcgt tttttattaa tattttttga atacatattt ataaatgcaa atttaatata
    19261 attattgaat gttaaaaatt tttaacaaaa tattagtttc aatctaaatt attttgtttc
    19321 agtttattcc ctttcaaatg ttatgttatt gtttaaaaca tattttatca atccgtattt
    19381 tttcgcaaca aaaatttctt ttacagtaaa atctttccat tgttctgtaa aatctttaaa
    19441 ttcaaaatgt aaaacttcgg aatagttaga gttaccaaca acataagaat caagaatttc
    19501 ggcagttgat ttaatttgcg tattgtcgtc aagttcttcg tatgttgagt ttgacaaatt
    19561 cccaaatggt gtataaatgc gagaaatagt tttgtttttt aaatcgtatg caaagcaaat
    19621 atcattaagg tctatataaa tttcatcgcc ttcgtcatca ttataaaaag ctgctgttag
    19681 cgacaatgaa aattttgcac tataatttga agtaaattct tgaaaatgat attccgtatt
    19741 tgctctccta gttgtaaaaa aaagaattcc tccagcagat tcagtaaaat aataattgtt
    19801 actttgcaaa gtaaaactat gagatatgtt attattatct ttcataacaa aactatcacc
    19861 aatcttgtag ggcgttatcc aatctttgtt ttcaggagaa aaataattag ttctctcttt
    19921 tacacaagca gttagcaata ttaaagcaag aaacacaatt cctttactcg cacaacgtat
    19981 agtattcatt atttgaaagc gttattttta taataccttt atcataactg taatacatgc
    20041 tgtcagcata aattgagtca tgtgaggaat atctgtcgta gtaagatttt aatttaaaaa
    20101 cttggtaata atatactgaa tcaattttaa ttgaatcaac ttgttcgtaa taattagtat
    20161 caaaattgcc agttggtatg tgaaatccaa attttcctaa ggatacattg cagttatagt
    20221 ttaaagtgtc taagttgcag atattaagtt caaaattaaa aatcggataa tcaggaatta
    20281 atctcgtagt attacactca tagtgaatta cataaggaca agacctcatg caactagacc
    20341 attcctcacg tttttgtgtt tctctactag catcaaaatt tactacaaga ccgtttgagt
    20401 gcttaaattt atatgtttta ccgctaatat atgggacata ttctaacgcc gattcgggca
    20461 aaactccatt gtcaattact gttgtttcgc aaggcggacg accacatgct cgcaacaaaa
    20521 gcattgataa taaacaaaat ccaaaaatct gtaatttata aagcataatt cttaaaattt
    20581 gatacaaaga tatagtattt aaacgtataa aaagcactta ttattaaaaa tatataggtt
    20641 tttttaaagc atttttttat tttagcagta aattttttgt gaatgaagtt tgttaaaaaa
    20701 ccacataatt taatagcagt tcttaccgct gtttttagtt ttattattta tgttttaact
    20761 ctgtcgccaa cagtaaattt ttgggactca ggcgaattta tttgctgtgc taatacttta
    20821 caaataaacc atgctcctgg aaatccaact tatctgcttc ttgccagaat tttttcaatc
    20881 tttgcttcaa gtccacaaaa aattgctttt gcaataaact tgttttctgc tttttcggct
    20941 gcttttgcaa tattttttgt ttttaaaact attttttggt ttagtgaaaa attaattgca
    21001 aaaaagagta atttagaccc taaaaattct tacttagttt caattcttgc atcaataatt
    21061 ggagctctaa gtttagcttt ttcttcaagt ttttgggcaa tagcaaccga agcagaagtc
    21121 tattctcttt ctttgttttt cacatcttta tgcctttggt tgatgacaaa atatttagat
    21181 gaggcaaacc aaaaagcaaa taatttgata gttttaatcg cattaatgct tggattaagt
    21241 cttggagttc atcaacttaa tattttgata attccagctt tagtgttttt attttatttt
    21301 tataaatgta aattttctta taaaggattt gcaattgcaa tagttttggc tattggaata
    21361 ttaatttttg ttcaaatttt tgttgcaggt ttaccacaaa ttttagcttg gttcgagaaa
    21421 atatttacaa atcacttatc tgctggatat tatgttggct taattagttt ttcgttaatt
    21481 tttattttgc ttataggttt gggaatttat tggtcaggag taaaaaataa aaaagtgtta
    21541 aacttaattt ttacaagttt tttaatgttt gtaataggct attcaagcta tgcaattatt
    21601 ataatcaggt cggcatctaa tccaccaatt gaccaaaata atgttgaaaa tatttacaat
    21661 tttgtttctt acttaaatcg cgaacaatat ggcgaccgtc ctttatggag aggacaacaa
    21721 ttttcgagca aattagataa agcaaaacct tacaaaaaag gagaagcaat ttacgataaa
    21781 actaatggta agtaccaact tgttcaccat aagccagtta ctaactataa aaaatcagat
    21841 attagatatt tgccaagaat gtggagcgat aatcctcttc atatttatgc ttattcagaa
    21901 tggacaaata ttacaaatag tgaaattcca cgagcttcgc aaaattttgg ttttatgttt
    21961 cgctatcagt tcggacatat gtatttgcga tatttcatgt ggaactttgt tggaaaacaa
    22021 aataatattc aaggacatgg agatgcaatt catggaaatt ggataagtgg aataaaattt
    22081 attgataatt ttcgtttggg aaatcaagag aaaaaaccag attttttaaa gaaaaatcaa
    22141 actgcatatt attttctacc ttttatttta ggacttatag ggattttttc acaattaaaa
    22201 ttcgataaaa aaaatctgtt tgtgatttct gtgtttttta tttttacagg tattgcaatt
    22261 gctttttact taaatcaaag tccctaccaa gttagagaaa gagattatgc ttttttggga
    22321 tctttttatg ccttcacaat ttggattggg ttaggtatta cgggcttata tttgttttta
    22381 cacaaatttt tgaaaaagaa atttttaatt tttgctttgg gaattttagg aattttaatt
    22441 tctccagctc aaatgctttt gaaaaattac aaaattcaag ataaaaccaa caacgatttt
    22501 gcttatcatt ttgcacgaaa tttattaaat acttgcgacc aaaacgctat cttgtttgtg
    22561 tctggcgata acgaaacttt tccactatgg tatttgcaag aatgtgaaaa ttttagaaca
    22621 gatgtaagag taataaattt aagtttttta aataatgatt ggtatataga ccaaattcaa
    22681 agagctgagc gtggaaataa agctataaaa ttaaatttag ataggcgtaa gtatatttca
    22741 ggaaaagctg atattctgcc gattatagac aatactgcaa aatactattc ttttatttat
    22801 gataaagaat atgaaaaaat tgacgaagag tatagccaaa ttcatgatga gttttgcagt
    22861 ttattgaaaa aatctggtta tgccaataaa tataaattag aatttgaaag tttcgaagaa
    22921 tattttagaa atataaaagc ttttggtaac aacgaaaact ttaaagattt ttgttcaata
    22981 atttataatt tagaagacga tgaaattcaa aaatattatg gcattactga gcaacaagca
    23041 aaaaaactta tttcagattt agacaatttt ttaacttctc aaattttata tcttggaaat
    23101 ttagaatcaa aacttgaatt tatgttttcc gaagacacaa gtttaatgat tgcgtcaaaa
    23161 ttttatgatt acccaactaa ttattgtcca ggttatgaat ttaatatgaa gttaaataaa
    23221 tcaaaagcaa taaaatattt tgagccaata ggcttgcgtg aagaactttt ggtcgataaa
    23281 ataatttggc aaattgatac aagtcaaaaa tatttgaata aatctcaact tattgcatat
    23341 cagattatta atgagaacaa ttgggaaaga ccaatttatt tctcatcgtt tttagataag
    23401 gaaaattatt ttggattaga atcatattta tatttagaag gtttggctta cagacttttc
    23461 ccaataaaaa cagaatttac aaccaatgat ttagttaacg taaattctta taaaatgtat
    23521 gataatttta tcaacaaatt caaatggggc aagctaaatt atattgatga aagcatagaa
    23581 aatatattat ttcttctgag agcagattat acaaaattat caagaggttt gtttttagca
    23641 caagcttatg atgctgcgga acaagtaatt tcacattgca taaaagtaat ccctaataaa
    23701 aaagttaact ttgattatta tacggttgga ttagtacatt catattacag attaagaaaa
    23761 tttcccgaag caagtatatt aactttaatg atagcagaaa atgttgaaaa ggaattagag
    23821 ttttataata gtctttctgt tgaattaaaa agtggattaa cacaatctta tataaagcca
    23881 aaacagactt tagaagaact aatgatttta gcgaaacagt atgaaaaatc agaaaataca
    23941 gaaacttata aaaagctaaa acaaatttat gataaaacaa ttaatctaaa atagagtttt
    24001 aacttatgag ttttttgcca attagatatc ctaaactatt ttattgcaaa gctttgtcgc
    24061 gaaaaaaaga cagaccaaaa actctatatt taagctttga tgatggacca aatcctttgg
    24121 taacagaaaa agttttagaa attttaaaaa aatacaaggc aaaggctagt tttttttgca
    24181 aagggaaaaa tgcagatctt tatccagaaa tttttaacaa agtaaaagcc gaaggtcata
    24241 ccattggaaa ccattcttac atgcatttag acgcttttaa gcataaaaat aaaaaatggc
    24301 tatctgatgt gttgagaaaa tcgccagtat cggaagctta ttattttcgt ccaccttatg
    24361 gacatatttt cccttggcaa tatagagttt taagaaaaaa atataaatta gttttttggg
    24421 atgttatggt ttatgatttt cgaactgatt attcgttaaa aaaaataaaa aaaatattat
    24481 ttaacaaagt tagagatggc tctataatag tttttcatga taatattgca agttctcaaa
    24541 aaatgttacc agctttagaa tttacattag aatattatac aaaacgtgga tataaatttg
    24601 aaaaattata aaaaactaaa aacttttgtt agatttactt ttctaaaaat tgaattatga
    24661 aaataataag ttttaatctt aatggcatac gctcagcaat aaacaaggga ttttacgatt
    24721 ggttaaaaaa tgaaaaccct gatattttgt gcgttcaaga aactaaagca caagaatcgc
    24781 aaatcgaagt tgataaattt aaagagttag gttatcattc tataattcat tcagcagtaa
    24841 aaccaggata tagtggagtt gctatttttt caaaaaaaca agccgatttt gtgcaaatag
    24901 gaattggtat tgacgaattt gattatgaag gtcgccttat tagagccgat tttggagatt
    24961 tcactcaaat aaattcttac tttccttcag gaactatggg cgatgttcgt caagaagtta
    25021 aatatgcata tttagatgct gttcaaaatt atgttaatga gcttaaaaag gaaagaccaa
    25081 acattgtttt atcaggcgat tataatattt gtcacaaaga aatagatatt agcaatccta
    25141 aaacaaaaaa aggagtttca ggctttttac ccgaagaaag acaatggatg accgactttt
    25201 tagaaagtgg atttatagat tcgtttaggg tgtttgacca aagtcctgaa aaatatacat
    25261 ggtggagcta tagagcagga gcaagaaaga aaaatttggg ctggcgtata gactaccata
    25321 tggtttctga accaatgaga aataaattaa tttccgcctc aatacaaaac gaagtggtaa
    25381 tgtcggatca ttgtccgata gtggtggagt tggggtgatt ataattcaag attaacttac
    25441 tttcgaacat tgggcttaat gaaatgcg
//
LOCUS       DAOPNX010000027        25424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_2463_length_25424_cov_6.954590, whole genome shotgun sequence.
ACCESSION   DAOPNX010000027 DAOPNX010000000
VERSION     DAOPNX010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25424
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2463_length_25424_cov_6.954590"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..1811)
                     /locus_tag="PKN53_04485"
     CDS             complement(45..1811)
                     /locus_tag="PKN53_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOD88244.1"
                     /translation="MKIFEANISLQVCEFLKDFFNNTSSFEIANQKILDFFGINDFFE
                     NDNDFKELKKSVSIVSINSVAEPDRAEYGDFQTNKDLANKLAKSLSKNNILPEIIIEP
                     TCRKGNFIIASLSNFKTVKKVFGIEIYKPYIWETKFSILDYFLSNPNDNKPEITIKHC
                     NIFDFDFKNISKQYPNEKLLIIGNPPWVTNSKLGSLNSTNLPKKSNFKNQNGLDAMTG
                     KGNFDIAEYITLMLLDVFQTHNGHLALLVKNSVIKNIIFDQKDAKYKIGKIEKYCIDS
                     KKEFNVSVEASLFYCQLNSTPSFQCNELDFYSLEKKSSFGWLNAKFVSNLANYNENKE
                     IDGQCPFKWRQGIKHDCSNVMELERENECFINKLSQKIKLEEDLIYGFLKSSDLKNTV
                     IQNTRKQVIITQTKVGQDTSYIQHLYPKTYNYLKSNITYFQARKSSIYKGKPHFSIFG
                     IGDYSFKSYKVAISGLYKTYHFTLVLPQDNKPIMLDDTCYFIGFDKIEFAVYTLILLN
                     HSKTEEFLKSITFSDAKRVFTKDILMRLDLLNITKTISQKEIEEQINFLNNKYDLSIN
                     LSHWNSFINTITPQKAKKLTIF"
     gene            complement(1811..2485)
                     /locus_tag="PKN53_04490"
     CDS             complement(1811..2485)
                     /locus_tag="PKN53_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019036757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOD88245.1"
                     /translation="MKEKLTIAKLKAEAKAFCIAESKIQNKFLFGVTDRKAVGTHIKH
                     KFQEQLNSKYKTTIGSSASGIDLPSKDILTDIKVTSIKQPQSSCPFKDAKQKIFGLGY
                     NLLIFVYDKIDDPKTRTAKLDFVSCLFISKERTADYTTTYRLREMVKDKANIEDIVAY
                     LSDKNIPADEITLNQLAKQILKTPPVQGYLTISNALQWRLQYQRVVTLDKNVQGISKV
                     IDKASK"
     gene            complement(2551..2700)
                     /locus_tag="PKN53_04495"
     CDS             complement(2551..2700)
                     /locus_tag="PKN53_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88246.1"
                     /translation="MRAKIFLIILMSLYIDNSLFSQSNDCLKDFDYLVKKIQADYPGY
                     LELLD"
     gene            complement(2721..3767)
                     /locus_tag="PKN53_04500"
     CDS             complement(2721..3767)
                     /locus_tag="PKN53_04500"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOD88247.1"
                     /translation="MKKFFKPILAFFLPIIISINSFAQIPDGYYDGVQGLTGESLRSA
                     LHDIIDGHSVISYNGLWTAFKYTDNLGNNKVWDMYSNCNFTFGTDQCGNYQNICDCYN
                     REHSFPKSWFNDASPMYSDLFHLVPTDGKVNGYRSNYPFGECSSGTTYGTGKLGICTF
                     PGYTQTVFEPANEYKGDFARIYFYMLTRYMDKISSWSSPALSGNNFSEWTRKLLLKWH
                     RQDPVSQKEIDRNNVIYNDYQHNRNPFVDNPEWADMIWDPNYTPSFVESSKIYFDIFP
                     NPANDFINISFTQNFNFNYTINISDNSGRVIKSYTLNSNHENIDISELSAGSYFIVLN
                     SENITFVRQFFVVE"
     gene            complement(3764..4360)
                     /gene="purN"
                     /locus_tag="PKN53_04505"
     CDS             complement(3764..4360)
                     /gene="purN"
                     /locus_tag="PKN53_04505"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOD88248.1"
                     /translation="MKTINLALFASGSGTNVENIANYFAKNPQINISCVLCNKPDAYV
                     IERAKKLKLDFLVFNKADFQKNGKVECYLLEKNVNFIILAGFLWLIPEWLIEKYPNKI
                     VNIHPALLPKYGGKGMYGDNVHKAVLENKEKETGISIHYVNKNYDEGDIIFQAKTTID
                     ETDNIESIAQKVHELEYKFYPQVIENILLTLLPNIFVL"
     gene            4518..4754
                     /locus_tag="PKN53_04510"
     CDS             4518..4754
                     /locus_tag="PKN53_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOD88249.1"
                     /translation="MSDITSRVKSIIVNKLGIDESEVVPEASFTADLGADSLDTVELI
                     MEFEKEFGISISDQDAEKIATVGDAISYMEANVK"
     gene            4782..6032
                     /gene="fabF"
                     /locus_tag="PKN53_04515"
     CDS             4782..6032
                     /gene="fabF"
                     /locus_tag="PKN53_04515"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOD88250.1"
                     /translation="MTLKRVVVTGIGSISPVGANAKETWKNVVNGVCGIDLITHYDTT
                     NFKTKFACEVKNYDPLQYFDRKELRRIDKFTQFAIIASDEAIKDSAIDFESMDKSRAG
                     VIWSSGIGGVETFLEEIKGFIEGNYIPRFSPFFIPKIISDIAAGHISMKHGLMGPNYC
                     TVSACASSSNAIIDSFNLIRLGMVDYMVTGGSEASLNEVGMGGFQSMMAISTNNEEYK
                     TASRPFCATRDGFVMGEGGAGLILEEYEHAKARGAKIYAEVVGVGLSADAYHLTAPHP
                     EGLGANYVMRNALQDAKLKPEDIGYINVHGTATPLGDIAELKAIMQVFGDHAFKLNIS
                     STKSIVGHLLGAAGAMEASFAVMALVDQIIPPTINHQKADENIDPRFNLTLHKAQKRE
                     LTYAMSNTFGFGGHNTCVIFKKAE"
     gene            6038..6751
                     /gene="rnc"
                     /locus_tag="PKN53_04520"
     CDS             6038..6751
                     /gene="rnc"
                     /locus_tag="PKN53_04520"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOD88251.1"
                     /translation="MGFFFFKRFSEEDKKLLKNLKLILDFKPKILELYKVVFTHASVG
                     RNTKNNERLEFVGDSVMGLIITDILYNKFPKADEGRLSILRASIVSRQSLNVLGENLG
                     LDQLVDYKNTSGNEIKNLSGNALEAMVGAIYFDRGYHSAKKFVEKLVYENFDLEKLLE
                     QNKDYKSKLLQLIQKFKLNITFNTYENMESNEKNQHFLCELCLDDRFLAEGKAWTKKE
                     AEQKASKQALQKLSHLRLN"
     gene            6831..7250
                     /locus_tag="PKN53_04525"
     CDS             6831..7250
                     /locus_tag="PKN53_04525"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HOD88252.1"
                     /translation="MKTKKIKYEAEYNFKLIGISSSEDDYRLSWKLGKIFKAEFIKQE
                     DIEIKDNKYTETQKFVSYKCQPSNLSLNLRLVVNKTNIGYIVDELKNIDYFLQVFDSH
                     DDAYLEKIISEIKAISSVSAVFNLDLSRIKSKEKLLF"
     gene            7252..8748
                     /locus_tag="PKN53_04530"
     CDS             7252..8748
                     /locus_tag="PKN53_04530"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOD88253.1"
                     /translation="MGIVSRQTIKGSIYSYLGAFIGFVNVALLMPRLFATDQIGLVNI
                     LVALSTIFGQLGTLGMVNVTIRQFPYFRDEKNNHNGFLFFALAVGSLGFVLYTIFYYL
                     TKGYVIRTNIDQSPLFAEYVYLLLPFVFITIFYFIIDTYNRLLFNASFGAFAKELLLR
                     LLNFITIVLFWLELIDFHQFIILYTISYAVPVILICILLICKKQFSLKPSFKLFTKSF
                     TKEVISVAVFGLISSFSGMAVLQIDRYLINHYCDLSATGIYSTVFFFGTIILLPGRSL
                     IRISSTLITEAIQKENWKDVNEIYKRSTTSMSVVGMALFVLIWGNINNIMTILPPEFG
                     IGRYVIFFIALAHLLQMIASVSSEIIYSSKYYREFAVLMIILIISIIIFNIILLPKWG
                     IVGASIALALAFLIYLVSRQFFIYWKFKLNPYKLKHIIILIAGLVALIINVFIPQMHF
                     ILDICVRSLVLAIVFFVPILITKVYPELNSSMKNIVYKLKKLSQKSAK"
     gene            8815..9135
                     /locus_tag="PKN53_04535"
     CDS             8815..9135
                     /locus_tag="PKN53_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88254.1"
                     /translation="MGFGSFFKVPQHNVFDYKPRYYDPEKERRRELLNEMRIAQGKEP
                     VGMDSNKPGAKIKGSFKRPMKRTAYSKGNSTIRVLVIACILAFIVYLILIADLTPIVK
                     LFMK"
     gene            9135..10973
                     /gene="mutL"
                     /locus_tag="PKN53_04540"
     CDS             9135..10973
                     /gene="mutL"
                     /locus_tag="PKN53_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOD88255.1"
                     /translation="MADIIKLLSDSVANQIAAGEVIQRPASVVKELVENAIDAGATEI
                     KIAIIDAGRTLIQVSDNGKGMSPQDARTAFERHATSKISSADDLFAIRSMGFRGEALA
                     SIAAVASVELRTKTEGQDLGTCINISGSKVESQEVVNCPKGANFIVKNLFYNVPARRK
                     FLKSDPVENRHITTEIHRIVLANPEIAFQFMLDNKIVLTLNPENHKQRIVNVFGKSIN
                     QHLVSLNTETSIVKISGFVAKPERTRKTTNEQFFFVNKRFMIHPYFRKAISIAYEKLI
                     PEGEFPSFFVYLEIDPSTIDINIHPTKTEIKFEEEQSIFQILKASVKEALGKFNIVPG
                     LDFEENITRDVHLTSNTSFKPPTININPDYNPFKSHTQHKGGQSNYVPRNWSSLYEQS
                     QNKNREDFVLPQQENIEETQNLLSRDETQSTSFNKSLFFQLKNKYIITSVKSGMVVID
                     QKRAHERILYENFLKLLETRKGVSQRTLFPVIIEPSPTERAVILEIITDLNNIGFEIV
                     LVGKEKFELRAVPGDLTDIDPKKLIDEIVYAIAELQGSAKVVLNEKIALALAKTSSIR
                     VAKPLTELEMQEIFQKLMLCSDHNYTPEGKKILEVIKIEEIEQKLN"
     gene            10980..11714
                     /locus_tag="PKN53_04545"
     CDS             10980..11714
                     /locus_tag="PKN53_04545"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOD88256.1"
                     /translation="MNFQRGGFFNLPPVVKTLLIINIALFALRFLVPDSIMHLAVFSF
                     KSDFFRPYQLITHMFMHGNFWHLFFNMFALYMFGRILEEVWGSKRFFIYYFFTGLGAA
                     FLHLIVMNIEINSLIAKMDPQQVKYVLDEGSEALMQGKNFVDIQMRDLNLMINTPTVG
                     ASGAIYGLLLAFGMMFPNAILYLYAAIPIKAKYLVMIFVAIELVLGFLNRSGDNVAHF
                     AHLGGMLFGLIFILVWRKKQFNRWDQ"
     gene            11711..12616
                     /locus_tag="PKN53_04550"
     CDS             11711..12616
                     /locus_tag="PKN53_04550"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOD88257.1"
                     /translation="MSFLDELKYSFKAKNNFNILIYINLIVFLFIQLFKVFLFLNAKT
                     SQEFLYYLAVPANINNLKTYPWTIITYMFTHESFLHILFNLLAFYWFGKLFLQYLSQK
                     QLLGVYILGGILGAVFYILAFNIFPVFNEIKEISIAIGASASVMAIVFTVAMLAPNQE
                     IHLAFLGNFKLKYLALFVVILDILSIPTDNAGGHIAHLGGAFFGVMFALFYRKGVDIT
                     SFLTKFLYWIKNLFSPQKNMRIKYKKSKSKTKSTSTKRESDWDYNARKKAEQEEIDKI
                     LDKIAKSGYEALTASEKEKLFKQSK"
     gene            12613..13710
                     /locus_tag="PKN53_04555"
     CDS             12613..13710
                     /locus_tag="PKN53_04555"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOD88258.1"
                     /translation="MKKKKANKALLIINILASILLLAIYGIEFIPPSKFLFLSLLPFL
                     FTIILIVNIIFVLIWIFVRWKYSLISLISILLVIKYVALIYPIQSFFSSKSKEADFKV
                     MTYNVMIFGLYDWENTNIRTDIIQLISDENPDVLCLQEAYWNNSNSNFKTLKIILENL
                     NTKYVHKATMTTAKGGQNFGLVTISKFPILNSYSEKFDNSYNGFTYSDILIDADTVRV
                     YNVHLQSLEFNQRDYTVIENISEGINDLGVKTILKKYLKSIKIRIKQAEIVRGTMNDC
                     HYPILVCGDFNDIPISYSYLKLAKNLKDSFSACGRYPGYTWVNSKLKLRIDYILYSKD
                     FKCCSHKLIEKNLSDHYPVIAEFRFAKNDKQ"
     gene            complement(13723..13953)
                     /locus_tag="PKN53_04560"
     CDS             complement(13723..13953)
                     /locus_tag="PKN53_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOD88259.1"
                     /translation="MDEIIKKIEKSIDEVRPYLQADGGDISLIEVTPDYIVKVKLLGA
                     CGSCPFSIQTLKLGVEDKLKKDVPQVKEVVSV"
     gene            complement(13967..15070)
                     /locus_tag="PKN53_04565"
     CDS             complement(13967..15070)
                     /locus_tag="PKN53_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOD88260.1"
                     /translation="MKRNKENIEQVLTTVLHPETGKDIVSSGILEGIELTDNSVDLTI
                     SFFKSTDPFINSIKRAIKSALEAEFGTDLIVNITTKVKKKVKDPSSISAGLSEVKNII
                     AIASGKGGVGKSTVAVNLAIALAQKGFKVAVLDADIFGPSIPKMFGAEDARPSGYEED
                     GKEYIIPIEKYGIKLLSIGFFVSPKEATIWRGPMAGGALKQMIEQTKWGELDYLLFDL
                     PPGTSDIHLTLVQSVAVTGAVIVSTPQEVAIADARKGVAMFNASAIEVPVLGLVENMA
                     WFTPAELPENKYYIFGKGGVEKLAKEVNSEVLGQIPLVQSVCESGDKGTPAALEFSSV
                     IGKAFANLADNFVKAVEKRNADLPPTQQVIMNN"
     gene            complement(15193..16482)
                     /locus_tag="PKN53_04570"
     CDS             complement(15193..16482)
                     /locus_tag="PKN53_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOD88261.1"
                     /translation="MFKSILKKEKKISVIGLGYVGLPIALEFAKFVKVVGYDIKPERI
                     ELMKNKIDPSNELDKEAFENADILFTANPEDLRECHFHIVAVPTPIDEHKLPDLRPIL
                     SASKTVGKILKKGDYVVFESTVYPGCTEEDCVPILEEMSGLKFKKDFKVGFSPERINP
                     GDKQHTLPNIVKITSGCDAESAEEIAKVYEIIIKAGVHRTSSIKVAEAAKIIENTQRD
                     VNIALMNELSIIFDKIGINTFEVLEAAKTKWNFLNFTPGLVGGHCIGVDPYYLLHKAK
                     QLGYHAEMIDSGRSLNDSMGPYVADKAVKLIIANKKEITTAKILLMGITFKENVSDIR
                     NSRVIDIIEELQKYNITVDVVDAFAKPKEVKHEYNVDMIKEPKGKYTAVIVAVNHKPY
                     LNLEEKDFANLLVDNGVLIDIKGIYRNKIKNLTYWSL"
     gene            complement(16518..17462)
                     /locus_tag="PKN53_04575"
     CDS             complement(16518..17462)
                     /locus_tag="PKN53_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOD88262.1"
                     /translation="MSNLNFALVGVGGYIAIRHVKAIKETGNELVACLDPFDSVGFLD
                     AHFPNADFFHEFERFDRHVYKLSRTDKKIDYVSICSPNYLHDSHIRFALRNQAHAICE
                     KPLVLNPWNIDALAEIEKESGKKIYNILQLRLHQSIKNLKEKIENGPKDKIYDIDLAY
                     ITSRGKWYHYSWKGDIQKSGGIATNIGIHFFDMLSWIFGEVKSSVVHVSEKDKAAGYL
                     ELERARVRWVLSIDENMLPDEIKSKGQRTYRSIKMENEEIEFSDGFTDLHTESYRHIL
                     SGKGFGLKEAKPSIQTVFNIRNATPIGITGDYHPLLKK"
     gene            complement(17468..18043)
                     /locus_tag="PKN53_04580"
     CDS             complement(17468..18043)
                     /locus_tag="PKN53_04580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOD88263.1"
                     /translation="MKQDYFAHETAVIDEGCIIGKGTKIWHFSHIMSNCRIGENCNIG
                     QNVVVSPDVVLGNNVKVQNNVSIYTGVSCEDDVFLGPSMVFTNIINPRSAIVRRDSYL
                     KTKVKKGASIGANATIVCGHDIGRYAFIGAGAVVTKAVPDYALVVGNPARQTGWMSEY
                     GHKLNFDKNGFATCPESGEKYQLINNSVKKL"
     gene            complement(18102..19784)
                     /locus_tag="PKN53_04585"
     CDS             complement(18102..19784)
                     /locus_tag="PKN53_04585"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOD88264.1"
                     /translation="MKRFNLISFIFAIYIILTIASCAKQIAISGGPKDTSPPKFVKSE
                     PQNGSTNFNSDKILIQFDEYIRLNNVNQKLIVSPPMPTKPVVTIKRKGVLIKLKSNEL
                     EENTTYTLNFNDAIADNNENNVLNSFVFAFSTGDKIDSLLCGGVVLDAFDRKPVKDAW
                     VSLYSDLSDSAIIKSQPSYITKTDQNGKFLIPFVKENEYKIYALIDNNYNYLFDLPEE
                     SIAFLDTTIQPSVKLIAKTDTTAAKTINKPTNLELLIFKEDNQTQFIKSSKRINPRLV
                     EIVFNKPQHDNFEINVIGDENAILKMSKSLDTLQVFLTKKEIIDSSRMQIICNYPDFE
                     SSINIVTDSLFLRSVDKKLEDNIVKLSIPKTKEPLLDLEIKTEFPISEFDPSKLKLEL
                     ISENEYIKTDFKLEKDSINPLILALKSDLLEKSEYRLLADSAFLKNIYNEPNLVDTLK
                     FQTASSSEYGNFKIIFPEANTNYLVQMIQNEKVVAESSEENKEVVFEFLKAGKYKVKV
                     ILDSNQNGRWDTGNYIKNQHAEKVYFLPSEYEIRANWSHEIEWDPISNKFIE"
     gene            complement(19826..20875)
                     /gene="ribD"
                     /locus_tag="PKN53_04590"
     CDS             complement(19826..20875)
                     /gene="ribD"
                     /locus_tag="PKN53_04590"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOD88265.1"
                     /translation="MNSIDEIFMQRCLDLAQKGLGSVSPNPMVGSVIVHEGKIIGEGY
                     HRKYGEAHAEVNAINSVRDKSLLSKSTIYVSLEPCSHVGKTPACANLIVEKNIPKIVI
                     GTIDPHEKVAGKGIEILKTANREIITGVLENKCIDLNKRFFTFHQKKRPYIILKWAET
                     KDGYIDFVRTPSDKAKAFWITNDECKTLVHKWRTEEDAFLVGTNTVLLDNPQLTSRLW
                     KGKNPTRISIDKNLTLSKDFNIFNKEAETIIFNSHTTLIINNITYLKIDFSKNIIPQI
                     LENLYDFNILSLVVEGGTQTLQSFIDLNIWDEARVFVGDHIFEKGVPAPIFEKLEFKT
                     ETFGNSILKWFYNKY"
     gene            21055..23523
                     /locus_tag="PKN53_04595"
     CDS             21055..23523
                     /locus_tag="PKN53_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOD88266.1"
                     /translation="MRKVLLIVFIIVTTSFALFSQTVLLSGKVVDAENNEGLFGVNIT
                     INHAEELLANKQQLGTSTDFDGNYKIALYPGDYIVKFAFIGFEEVTKEVELLVGKPVV
                     LDINLSPTAEIIDEVVVSAGKFEQKLSDVTVSMEVLKPKMIESNNTTRLDHALNMVPG
                     VDVNDGQASIRASTGWSYGAGSRVIVLMDDLPIMSADVGDVKWYYVPLENVSQVEVVK
                     GASSALFGSSALGGVINVRSAYPTNKPVTKVSYFNGIYGNPRDFRQKWWGKEFFANNN
                     SPLKIPLREELFYFVRNPMYAGLSFHHAQQFDNLDLSLGGNHHIDEGYREAEYERRSR
                     FNANLRYRAKKVQGLAVGVNTNFMAVDKSDFFMWKADTNAYQANPGLGTTAPTQGFRM
                     NLDPYVNYYRPNGDKFSLRTRYFLTENVIPSDTMKNSKGYTYYGEFQYQTVLAEKWNL
                     AAGIVNSYSNVYSNMFGDHFSNNLSLYTHVDVTFGRLKLSGGLRGEYFVLDSTQTESL
                     FDIKLGKERLEIPFRPVFRTGVNYSVTDQTFIRASFGQGYRFPSIAEKYTATSLGMVN
                     VLPNPRLLPETGWSTELGIKQGYKFGKWRGFADLALFNQDINNMMEFTFGVFNPVTYM
                     PFQPTDTVIPVFAFQSQNYGDVRITGADLSASIAGKVGNYMIMGMVGYTYTYPVPKFG
                     ADTTASSLSPILKYRNRHSIKADIGVETKRITFGANIIWRSAMENVDRLFCDERDPAT
                     VNLTDIAFYQFFSQTILPGYWNYRIENAKKNYFNIDLRFGFNFNKYLSANFIVKNLLN
                     AEYVGRPGDMHAPRRYEILISAHF"
     gene            23553..25307
                     /locus_tag="PKN53_04600"
     CDS             23553..25307
                     /locus_tag="PKN53_04600"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOD88267.1"
                     /translation="MKHVIFTFFVVLFSLALNAQICDPNCTPDQSCVENFEGNGMICP
                     VDLPVATQNVFYDETVTVIPPKNFEGLSLIGAIRIDEVNGLPEGIAWCKSQEIFNVTT
                     PLTRYCAQLKGTPTTPGEYQLTLKITPYLNFFGTLVEQSPVYDDTSLLIVVLPYVEPP
                     VADFEANVTTIEQGQQVSFTDLSTNDPTSWSWIFEGGTPGASTEQNPTVTYMVPGTYN
                     VSLTVANAGGEDNVVKNAYITVTATPTPPVANFVGNPTTITVGGTVAFTDQSTNTPTS
                     WEWVFDGGEPATSTDQNPTVTYNTAGTYTVKLTATNVDGSDIKTRTNYITVVEDGSLT
                     ADFIGNPTTVEEGDTVYFTDQSLGNVTSWNWEFSGGNPATSTEQSPSVVYEIAGVYDV
                     KLTISDGTNQDEEVKVEYVTVIPQPEPVVADFTANPTSVEIGGTVNFTDQSTGGVTSW
                     NWQFPGGNPATSILPNPSVVYNTVGTYDVTLTVGNGTSQDEEVKTAYIAVIPSGIEGV
                     DFDFVKIYPNPASDNIVVEALGLEAVSIIDVLGKVVYTKTTNSDKEFIDVSKLQKANY
                     FIKVKTNSGEIVKAISVK"
BASE COUNT         8596 a         3754 c         3985 g         9089 t
ORIGIN      
        1 tgtttgatta aaataattag tatcattgtc aaagtccttt tttatcagaa tattgtcaat
       61 ttttttgctt tttggggtgt tattgtgttg ataaaactat tccaatgaga aagattaatg
      121 cttaggtcat atttgttatt taagaaattt atttgttctt caatttcttt ttgtgagatg
      181 gttttagtga tatttaataa atccaaacgc attaaaatat ctttagtaaa gactctttta
      241 gcatcggaaa atgttattga tttcaaaaat tcctctgtct tgctatggtt gagaagtatt
      301 agtgtataaa ctgcaaattc aattttatca aacccaataa aatagcaagt gtcgtcaagc
      361 attataggtt tgttgtcttg tggtaatacc aatgtgaaat gatatgtttt atacaatccc
      421 gaaattgcaa ctttgtaaga cttaaaagaa taatctccaa ttccaaaaat tgagaaatgt
      481 ggtttccctt tgtaaatact tgacttcctt gcttgaaaat atgtaatgtt tgattttaaa
      541 taattgtaag tttttggata caaatgttga atatatgaag tgtcttgtcc aacttttgtt
      601 tgggttataa taacttgttt tctcgtgttt tgaattacag tattttttaa atcagaactt
      661 tttaaaaacc cataaattaa atcttcttca agttttattt tttgtgaaag tttatttata
      721 aaacactcgt tttctctttc aagttccatc acatttgaac aatcgtgttt tattccttgt
      781 ctccacttaa aaggacattg cccatcaatt tctttatttt cattgtaatt agcaagatta
      841 gaaacaaact ttgcattaag ccagccaaaa cttgattttt tttctaaact ataaaaatca
      901 agctcattgc attgaaaact tggagttgaa tttaattggc aataaaacaa agaggcttca
      961 accgaaacgt taaactcttt tttactatca atacaatatt tttcaatttt tccaatttta
     1021 tattttgcat ctttttgatc gaaaataata ttttttatca ctgagttttt tactaataat
     1081 gctaaatgtc cgttatgagt ttgaaaaaca tctaaaagca ttaaagttat atattcggca
     1141 atgtcaaaat ttcctttgcc tgtcattgca tctaagccat tttgattttt gaaatttgat
     1201 ttttttggaa gattagttga gttaaggctt cctaattttg agtttgttac ccaaggagga
     1261 tttccaatta ttaataattt ttcgtttggg tattgtttag aaatattttt aaaatcaaaa
     1321 tcaaaaatgt tacaatgttt aattgtgatt tctggtttat tgtcgttggg gtttgaaaga
     1381 aaataatcga gaatactaaa tttagtttcc caaatgtaag gtttgtaaat ttctattcca
     1441 aatacttttt taacagtttt aaaattggat aaagaagcaa tgataaaatt tccttttctg
     1501 catgtcggct caataataat ttcgggtaat atgttgtttt tggatagact ttttgcaagt
     1561 ttgtttgcca aatctttatt agtttgaaaa tcaccatatt cagcacggtc aggttctgca
     1621 accgaattta tggaaacaat agaaacagat ttttttaatt ctttaaaatc attatcattt
     1681 tcaaaaaaat cattgatgcc aaagaaatcc aatatttttt gattggcaat ttcaaaagac
     1741 gaagtattgt taaaaaaatc ttttaaaaac tcacaaactt gtaacgaaat atttgcttca
     1801 aatattttca ttacttgctg gctttatcaa ttactttact aattccctgc acatttttgt
     1861 caagtgtaac aactctttgg tattgaagtc gccattgtaa agcatttgaa atggttaaat
     1921 atccttgaac aggaggcgtt tttagaattt gttttgctaa ctgatttaga gttatttcat
     1981 ctgcaggaat atttttgtcg cttaggtaag caacaatatc ttcaatatta gccttatctt
     2041 taaccatttc acgcaaacga taagttgttg tgtaatctgc tgttcgttct tttgaaataa
     2101 ataaacaact aacaaaatca agtttagcag ttctagtttt gggatcatct attttgtcat
     2161 aaacaaaaat aagtaagttg tatccaagtc caaaaatctt ttgcttagca tctttaaatg
     2221 ggcaagatga ttgtggttgc tttattgatg taactttaat atctgttaaa atatcttttg
     2281 atggcaaatc aattccactt gcagaagaac caatagttgt tttgtatttt gagtttaatt
     2341 gttcttgaaa cttatgtttg atatgtgtac ctacagcttt cctgtcagtt actccaaata
     2401 gaaatttatt ttgaattttt gactctgcaa tacaaaaagc tttggcttct gcttttaact
     2461 tagctattgt cagtttttct ttcattataa aaccttgtgt ttagtgtaca aatataattt
     2521 tttttgttat ttatttctct attattttcc ttaatctaat aattccaaat aaccaggata
     2581 gtctgcctga atttttttaa ccaaataatc aaaatctttt aagcaatcgt ttgattgtga
     2641 aaatagtgag ttgtcaatat aaaggctcat tagaattatt aaaaaaatct ttgctctcat
     2701 ataattttta aatatctaat tcactccaca acaaaaaact gtctaacgaa agtaatgttt
     2761 tcagaattta gaacaataaa atatgagcca gccgaaagtt cgctaatatc gatattttcg
     2821 tggttagagt ttagagtgta agattttatt actcgaccag aattatcgct tatatttata
     2881 gtataattga agttgaaatt ttgagtaaat gaaatattta taaaatcatt agctgggttt
     2941 ggaaaaatat caaaatatat tttagaactt tcaacaaaag aaggagtgta gttgggatcc
     3001 caaatcatat ctgcccattc gggattgtca acaaaaggat ttctattgtg ctgataatcg
     3061 ttgtatataa cattatttct atcaatttct ttttgagata ctgggtcttg tctgtgccat
     3121 ttaagtaata attttcttgt ccattcagaa aaattatttc cactaagtgc tggactgctc
     3181 caagatgaaa ttttatccat atatcttgtt aacatataaa aataaattct ggcaaagtct
     3241 cctttatatt catttgcagg ctcaaaaact gtttgtgtat atccgggaaa agtgcatatt
     3301 cctaatttgc ctgttccata tgttgtgcca gaggaacatt ctccaaacgg ataatttgat
     3361 ctatatccgt ttacttttcc atcggttggt actaaatgaa ataaatctga atacataggt
     3421 gaggcatcgt taaaccagct tttggggaaa ctatgttcac gattatagca gtcgcaaata
     3481 ttttggtaat ttccacattg gtctgttcca aaagtaaaat tacaatttga gtacatatcc
     3541 caaactttgt tatttcctaa attatcagta tatttaaaag ctgtccataa tccgttatat
     3601 gaaattactg agtgtccatc aataatgtcg tgtaatgcag aacgcaaact ctcgcctgtt
     3661 aaaccttgaa caccatcata ataaccatca ggaatttgag caaaagaatt aatgcttata
     3721 ataataggta agaaaaaggc aagtataggt ttgaaaaatt ttttcataaa acaaaaatat
     3781 taggcaacaa agttagtaaa atattttcta ttacttgagg ataaaattta tattctagtt
     3841 cgtgtacttt ttgtgcaatg ctttcaatgt tgtcggtttc gtctatagtt gtttttgctt
     3901 gaaaaataat gtctccttca tcgtaatttt tattcacata atgaatactt attccagttt
     3961 ctttttcttt attctccaat acagctttat gaacgttgtc gccatacatt ccttttcccc
     4021 catattttgg taataatgca gggtgaatat ttacaatttt attaggatat ttttcaatta
     4081 accattcggg aattaaccat aaaaaaccag cgagtatgat aaaattgacg tttttttcca
     4141 ataaatagca ttcaactttg ccgttttttt gaaaatctgc cttgttaaat accaaaaaat
     4201 caagttttag tttttttgcc ctttcaatta cataagcgtc aggcttattg caaagaacac
     4261 atgagatatt aatttgagga tttttagcaa aataatttgc tatattttct acgttagtgc
     4321 ctgagccaga ggcaaatagg gctaaattga tggttttcat tttatttcta ataatttacc
     4381 atgtttatat tgctgataat cagcgtattg tgcaaattgt taataaaata ttcattaaaa
     4441 aatttgagtt aacgaatgat ttttgtttct ttgcacctgc aaaactaatt aattttatta
     4501 atcttaaatt attaattatg tcagacatta catcaagagt aaaaagtatt atcgttaata
     4561 aattaggtat tgacgaatca gaagtagttc cagaagctag tttcaccgct gatttaggtg
     4621 cagattcttt ggacacagtt gaacttatca tggaatttga aaaagaattt ggtatttcta
     4681 tttcagacca agatgctgaa aaaattgcca cagtaggtga tgcaattagc tacatggaag
     4741 ctaacgtaaa gtaaattttg aataactaaa aaattcacag tatgacatta aagcgagttg
     4801 tagttaccgg catcggaagc atttctccgg taggcgctaa cgcaaaagag acatggaaaa
     4861 atgttgttaa cggcgtttgt ggaatcgact tgataacaca ttatgatact acgaatttta
     4921 aaacaaaatt tgcttgcgaa gtaaaaaatt acgacccgct tcaatatttt gaccgtaaag
     4981 aacttagaag aatagacaaa tttacacaat ttgctattat cgcttccgac gaggctatta
     5041 aggatagtgc aattgatttc gaatcaatgg ataaatcccg agctggagtt atatggtcat
     5101 caggtattgg cggtgttgaa acttttttag aagaaattaa gggatttatc gagggaaatt
     5161 atatccctcg ttttagccct tttttcattc cgaaaattat ttccgatatt gcagcaggac
     5221 atatatcaat gaaacatgga cttatgggtc caaactattg tactgtgtca gcttgtgctt
     5281 cttcgtcaaa tgctattata gattccttta atctaattag attaggaatg gtagattata
     5341 tggttactgg cgggagcgaa gcctcactaa acgaagtggg aatgggcgga tttcaatcca
     5401 tgatggcaat atctactaat aatgaagagt ataaaacagc gtctcgtcca ttttgtgcaa
     5461 cacgcgatgg atttgtaatg ggcgaaggtg gtgcaggtct tattctagaa gaatatgagc
     5521 acgctaaagc tagaggtgct aaaatttatg ccgaagtagt aggagttggt ttatcagcag
     5581 atgcatatca cttaacagca cctcacccag aaggattggg agctaattat gtgatgagaa
     5641 atgctctgca agatgctaag ctaaaaccag aagatatcgg atatataaat gtacacggaa
     5701 ctgctacacc tcttggagat attgcagaac ttaaagcaat tatgcaagtc ttcggagatc
     5761 acgcattcaa attaaatatt agctcaacta aatctattgt aggacaccta ctaggtgctg
     5821 caggagcaat ggaagcttca tttgctgtta tggctttggt tgatcaaata attccaccaa
     5881 caataaatca ccaaaaagca gacgaaaata tagacccaag atttaattta actctgcata
     5941 aagctcaaaa aagagaactt acttatgcaa tgagcaatac ttttggtttc ggtggacaca
     6001 atacttgtgt gatttttaaa aaagctgaat aatcttaatg ggcttcttct tttttaaaag
     6061 gtttagcgaa gaagataaaa aattattaaa gaatttaaag ctaatattag attttaaacc
     6121 taaaattcta gaattatata aagttgtttt tacacacgca tctgtaggaa gaaacaccaa
     6181 aaacaatgaa agattagagt ttgtcggaga ctctgtaatg gggctgataa ttactgatat
     6241 tttgtacaat aaatttccaa aagcagatga gggaagactt tctatactca gagcaagcat
     6301 agtaagcaga cagtctttaa atgtattggg cgaaaaccta ggattagatc aattggttga
     6361 ttataaaaat acttcaggaa atgaaataaa aaacttgtcg ggaaatgcct tagaggcaat
     6421 ggttggagct atatattttg acagaggcta ccatagtgcc aaaaaatttg ttgaaaaatt
     6481 agtttatgaa aactttgatt tagaaaaact tttagaacaa aataaagatt ataaatctaa
     6541 actattgcaa ctaattcaaa agtttaaatt gaatattact tttaatactt atgaaaatat
     6601 ggaaagtaat gaaaagaatc aacatttttt gtgcgaacta tgtttagatg atagattttt
     6661 ggctgaagga aaagcatgga caaaaaaaga agcagaacaa aaagcctcca aacaagcatt
     6721 gcaaaagctc agtcatttga gattgaacta aatttaaaat aatgcaaata atttaagcaa
     6781 actcttaaac aaagttatac tatacaaaaa aaataactac atttgtattg atgaaaacta
     6841 aaaaaataaa atacgaagca gaatataact ttaagcttat aggaatttct tcatctgaag
     6901 atgattacag gctttcgtgg aagttgggaa aaatttttaa agcagaattt ataaaacaag
     6961 aagacataga aattaaagat aataaatata cagaaactca aaaatttgtt tcatataaat
     7021 gtcagccttc aaatctttca ttaaacttga gattggttgt taacaaaacc aatataggtt
     7081 atattgttga tgaattaaaa aatatagatt attttttgca agtgtttgat agtcatgacg
     7141 atgcttattt ggagaaaata atttcagaaa taaaagcaat aagttcagtt tctgccgttt
     7201 ttaatttaga tttatcacga attaagtcta aggaaaaatt acttttttaa aatgggaata
     7261 gtatctcgac aaacaattaa aggttccatt tattcctact tgggtgcatt tataggcttt
     7321 gtaaatgtcg ccttgcttat gccaagattg tttgctacag accaaatcgg tcttgtcaat
     7381 attttagtgg ctttatctac aatttttggg caattgggaa ctttaggaat ggtaaatgtt
     7441 actattaggc aattccctta ttttagagat gaaaaaaata atcacaatgg ctttttgttc
     7501 tttgctttag ctgttggtag cttaggtttt gtgctgtaca cgatttttta ttatttaaca
     7561 aaaggttatg taataagaac aaatatagac cagtcgcctc tgtttgcaga atatgtttat
     7621 ttgttgttgc catttgtttt tattacaatt ttttatttca taatagatac ctataatcgt
     7681 cttttgttta atgcttcgtt tggagcattt gctaaagaat tgcttttaag gttgttaaac
     7741 tttattacaa tagtcttgtt ctggttagaa cttatagact ttcatcaatt tattattctc
     7801 tatactattt cttatgctgt tcctgttatt ctaatttgta tattattaat ttgtaaaaaa
     7861 caattttctc taaaaccttc atttaaactt ttcactaaat catttacaaa agaagtgatt
     7921 tctgttgctg tttttggctt gatttcaagt tttagtggaa tggcagtttt gcaaatagat
     7981 agatatttaa ttaatcatta ttgcgattta agtgcaactg gaatatattc tactgttttc
     8041 ttttttggta ccataattct attgccagga agatctttaa ttagaatttc aagcacatta
     8101 attacagaag caattcaaaa agaaaactgg aaagatgtaa acgaaattta taaaagaagt
     8161 acaacaagta tgtcggtggt tggcatggct ctttttgttt taatatgggg aaatataaat
     8221 aatattatga caattttacc accagagttt ggaattggac gttatgttat attttttatt
     8281 gcactagctc atcttcttca aatgattgca agcgtaagta gcgaaattat ttattctagc
     8341 aagtattatc gagagtttgc agtcctaatg ataattttaa taatttctat aattatcttt
     8401 aatattattt tactacctaa atggggaata gtcggagcta gcattgcctt ggctcttgca
     8461 tttttaattt acttagtttc taggcaattt tttatttatt ggaaatttaa acttaatccc
     8521 tataaactaa aacatattat catattaatt gctggacttg ttgctttaat tattaatgtg
     8581 tttattccac aaatgcattt tattttagat atttgtgtaa gaagtcttgt tttggcaata
     8641 gtattttttg tgccgatttt aataacaaaa gtttatccag aacttaattc ttcaatgaaa
     8701 aatatagttt ataagcttaa aaaattatct caaaaaagcg caaaataaac aaagtgttgt
     8761 cgggttagtg aaataattca attaaaaaca ttaaatttgt aaaaatttat aattatgggt
     8821 ttcggaagtt tttttaaagt accacaacat aatgtgtttg attataaacc acgctattat
     8881 gaccccgaaa aagaaagaag acgggagttg ttaaatgaga tgagaattgc tcaagggaaa
     8941 gagcctgttg gtatggacag taataaaccc ggagctaaga taaaaggtag ctttaaacgc
     9001 cctatgaaac gaacagctta tagtaaagga aattctacta taagagttct cgtaatagct
     9061 tgtattttag cttttatagt ttatttaata ttaatagctg accttactcc aattgttaaa
     9121 ctgttcatga aataatggca gatattatta aacttttatc cgattcggtt gcaaatcaaa
     9181 tagccgcagg cgaagttata caaagaccag cttctgttgt taaagagctt gtcgaaaatg
     9241 ctattgatgc aggtgcaaca gaaataaaaa tagcaattat tgatgcagga cgaactttaa
     9301 ttcaagtttc agacaacgga aaaggaatga gcccacaaga tgctagaact gctttcgaac
     9361 gtcatgctac ttcaaaaatt tctagtgccg acgacttgtt tgctattcgt agtatggggt
     9421 ttagaggcga agcccttgct tctattgctg ctgttgcttc ggttgaacta agaacaaaaa
     9481 ccgaaggaca ggatttagga acttgtataa atatttctgg ctcaaaagta gaatctcaag
     9541 aagtggtaaa ttgccctaaa ggagcaaatt ttattgtaaa aaacttattt tacaatgttc
     9601 ctgcaagacg aaaatttttg aaaagcgacc cagtagaaaa tcgacatata acaacagaaa
     9661 ttcatagaat agttttagca aatcccgaaa tagcttttca atttatgtta gacaataaaa
     9721 ttgttttaac attaaatccc gaaaatcata aacaaagaat tgttaatgtg tttggaaaaa
     9781 gtataaatca acatcttgta agtctaaaca cagaaacatc aatagtaaaa atttctggct
     9841 ttgttgcaaa acccgaacga acacgcaaaa caactaatga gcaatttttc tttgtaaaca
     9901 aacgctttat gattcatcct tattttcgaa aagcaattag tatagcttat gaaaaactta
     9961 ttcccgaagg agaatttcca agtttttttg tttatctcga aatagaccca tcaactattg
    10021 atattaatat tcacccaaca aaaactgaaa ttaaatttga agaagaacaa tctattttcc
    10081 aaattctgaa agcaagcgta aaagaagctt tgggaaaatt taatatagtt ccaggattag
    10141 atttcgaaga aaatataaca agagatgttc atttaacttc aaatacaagt tttaaaccac
    10201 caacaattaa tataaatcca gattataacc catttaagtc tcatacacaa cataaaggtg
    10261 gacaatctaa ttatgtacca cgcaactgga gcagtttata cgagcaaagc caaaacaaga
    10321 atagagaaga ttttgtttta ccacaacaag aaaatattga agaaacacaa aatttgcttt
    10381 cgcgagatga aactcaatca actagtttta acaaatcatt gttttttcaa ctaaaaaata
    10441 agtatattat aacttcggta aaatctggaa tggttgttat agaccagaaa agagcacatg
    10501 aaagaatttt gtacgaaaac tttttaaagc ttttagaaac tcgaaaagga gttagccaaa
    10561 gaacactatt tcccgtaata atagagcctt ctccaaccga aagagcagtt atcttagaaa
    10621 taattacaga cttaaacaat ataggctttg aaattgttct tgttggtaaa gaaaaatttg
    10681 aacttagagc tgtgccaggc gatttaacag atatagaccc caaaaaatta attgatgaaa
    10741 tagtttatgc aattgctgaa ttacaaggct ctgcaaaagt tgtgctgaac gaaaaaattg
    10801 ccttagcttt agcaaaaaca tcatcaataa gagtagctaa gcctttaaca gaactcgaaa
    10861 tgcaagagat ttttcaaaaa cttatgcttt gctcagacca taactatacg ccagaaggaa
    10921 aaaaaatatt agaggtaatt aaaattgaag aaatagaaca aaaattgaat taatttatta
    10981 tgaattttca aagaggaggt ttttttaatt taccaccagt agtaaagact ttattaataa
    11041 taaatattgc tctttttgca ttgaggtttc ttgtaccaga ttcaataatg catttggctg
    11101 tttttagttt taaatctgat ttctttagac cataccagct gattactcac atgtttatgc
    11161 acggcaattt ttggcatttg ttctttaata tgtttgcatt atatatgttt ggtaggattt
    11221 tggaagaagt atggggttcg aaaagatttt ttatttatta ttttttcaca ggtttaggag
    11281 cagctttttt gcatttaata gtaatgaata tagaaatcaa ttcacttatc gcgaaaatgg
    11341 atcctcagca agtaaaatat gttcttgacg aaggtagtga ggctttaatg caaggtaaga
    11401 attttgtaga tattcaaatg agagacttaa atttaatgat aaacacccca acagtaggag
    11461 cttctggagc tatttacggt ttgcttttag cttttggaat gatgtttccc aatgcaattt
    11521 tatatcttta tgctgctatc ccaataaaag caaaatattt agttatgatt tttgttgcaa
    11581 ttgagttggt tttaggtttc ttaaatagaa gtggtgataa tgtcgctcac tttgcacatt
    11641 taggcggaat gttgttcgga cttatattta ttttagtttg gagaaaaaaa caatttaatc
    11701 gctgggatca atgagttttt tagatgaatt gaaatatagc tttaaagcta aaaacaattt
    11761 taatattctt atttatatta atttaattgt ttttcttttt attcagttat ttaaagtttt
    11821 tttgtttctc aacgctaaaa caagtcaaga gtttttatac tacttagctg tccctgcaaa
    11881 tattaataat ttaaaaactt atccatggac aataattact tatatgttta cccatgaaag
    11941 ttttttgcat attttgttta atctattagc cttttattgg tttggaaaac tttttttgca
    12001 gtatttgtct caaaaacaat tacttggcgt ttatatttta ggaggaatat taggtgcagt
    12061 tttttatatt ttagcattta atatttttcc tgtttttaac gaaattaaag aaatttctat
    12121 tgcaatagga gcatctgctt cggttatggc tattgttttt acagttgcaa tgcttgcacc
    12181 taaccaagaa atacatttgg cttttttagg aaattttaaa ttaaaatacc ttgctttgtt
    12241 tgttgttata ttagatattt taagtattcc aacagataat gcaggtggtc atattgcaca
    12301 tttaggagga gctttttttg gagtaatgtt cgctctattt tatcgcaaag gagttgatat
    12361 tactagtttt ctcactaaat ttctttattg gataaaaaac ttatttagtc cccaaaaaaa
    12421 tatgagaatt aaatataaaa aatctaagtc aaaaacaaaa tcaacatcaa caaaacgaga
    12481 aagcgactgg gactataatg caagaaaaaa agcagaacaa gaagaaatag ataaaatact
    12541 tgataaaatt gcaaaatctg gttacgaagc attaacagca agtgaaaaag aaaaactttt
    12601 taaacaaagc aaatgaaaaa aaagaaagca aataaagcat tactaataat aaatatacta
    12661 gcaagcatat tattacttgc tatttatgga atagaattta tccctccatc aaaatttttg
    12721 tttctttctc tacttccatt tttatttaca attattttaa ttgtaaacat aatatttgtt
    12781 ttgatttgga tttttgtgag atggaaatat tctttaattt ctttaataag tattttgtta
    12841 gtaattaaat atgtagcttt aatttatcct attcaatctt tttttagctc aaaatctaag
    12901 gaagccgact ttaaagtaat gacttataat gtaatgattt ttggcttgta cgattgggaa
    12961 aatacaaata ttagaactga tataattcaa cttatctctg atgaaaaccc agatgtttta
    13021 tgcttgcaag aagcttattg gaataattct aatagtaatt ttaaaacttt aaaaataatt
    13081 ttagaaaact taaacactaa atatgttcac aaagctacta tgacaactgc aaaaggtgga
    13141 caaaattttg ggcttgtaac aatttcaaag tttccaattt taaattctta ttcagaaaaa
    13201 tttgacaatt cttataatgg atttacttat tctgatattt tgatagatgc cgatactgtt
    13261 agagtttata atgtacatct tcagtctttg gagtttaatc aaagagatta tactgttata
    13321 gaaaatattt cagaagggat aaatgatctt ggggtaaaaa ctatattaaa gaaatattta
    13381 aaatcaataa aaattcgcat aaaacaagcc gaaattgtta gggggacaat gaatgattgt
    13441 cattatccaa tacttgtttg tggcgatttt aatgatattc ctattagtta ttcttatttg
    13501 aaacttgcaa aaaacttaaa agacagcttt agcgcatgtg gacgctatcc tggatatact
    13561 tgggtaaatt caaaacttaa attaagaata gattatattt tgtatagcaa agattttaag
    13621 tgctgttctc acaagcttat agaaaaaaat ctatccgacc actatcctgt aatagcagaa
    13681 tttagatttg caaaaaatga caaacaatag attttgtttg acttataccg aaactacttc
    13741 cttaacttgc ggaacgtctt ttttaagttt atcttcaaca ccaagtttta gagtttgaat
    13801 actaaatggg caagagccac aagcacccaa tagttttact ttaacaatat aatctggtgt
    13861 aacttcaatt aaactaatgt cgccaccgtc tgcttgtaaa tatggtctaa cttcgtctat
    13921 tgatttttct atttttttga taatttcgtc catgatattt tattttttaa ttattcatta
    13981 ttacttgttg cgtgggtggt aagtctgcat ttcttttttc aacagctttt acaaaattgt
    14041 ctgctaaatt tgcaaaagct ttaccaataa cagagctaaa ttctaaagct gcaggagtac
    14101 ctttatcacc actttcgcat actgattgaa ctaaaggaat ttgtccaagc acttcactat
    14161 ttacttcttt agcaagtttt tctacaccac cttttccaaa aatataatat ttgttttcag
    14221 gaagttcggc aggagtaaac caagccatat tttcaactaa accaagaact ggaacttcta
    14281 tagcacttgc attaaacatt gcaacacctt tgcgagcatc ggcaatagca acttcttgag
    14341 gagtacttac aattacagca cctgtaactg caactgattg tacaagagtt aaatgaatat
    14401 cgctagttcc gggaggaaga tcaaacaata aataatctaa ttcgccccat tttgtttgct
    14461 caatcatttg ttttaaagca ccacctgcca taggtccacg ccaaatagtt gcttcttttg
    14521 ggcttacaaa aaatcctatc gacaataatt taattccata tttttcgatt ggaataatat
    14581 attctttacc gtcttcttca tatccactag gtcttgcatc ttcggcacca aacatttttg
    14641 gaatacttgg tccaaaaata tcagcatcta aaacagcaac tttaaaacct ttttgagcta
    14701 aagcaattgc taagtttaca gcaactgtag atttaccaac gccaccttta ccactggcaa
    14761 tagcaataat atttttaact tctgataatc cagctgaaat gctagaagga tcttttactt
    14821 ttttctttac ctttgttgtg atatttacaa ttaaatctgt tccaaattca gcttctaaag
    14881 cagattttat agctctttta atggagttta taaatggatc tgtagattta aaaaaactta
    14941 ttgttaaatc aaccgaattg tcagttaatt ctatcccttc taaaatgcct gaactaacaa
    15001 tgtctttccc tgtttcgggg tgtaataccg ttgttagaac ttgttcaatg ttttctttat
    15061 ttcttttcat atttaaaatt aactattata ctagtaacaa aattgtttac tacttgttct
    15121 tttagagtac aaacttacaa attaaaaaat aaaaaagcta tagtttttta tgacattata
    15181 caggattgaa aactataaac tccagtaggt taaattttta attttatttc tataaatacc
    15241 tttaatatca attaaaacgc cattgtcaac taaaagatta gcaaaatctt tttcttctaa
    15301 gtttaaatat ggtttgtgat ttactgcaac tattacagca gtatatttgc ctttaggttc
    15361 ctttatcata tcaacattgt actcatgttt cacttcttta ggttttgcaa atgcatcaac
    15421 gacatcaact gtaatattat atttttgtag ttcttcaata atatctatta ctcttgaatt
    15481 tcttatatcg ctaacatttt ctttaaatgt tatacccatt agtaaaattt ttgcagtagt
    15541 aatttctttt ttgttagcaa taattagttt tacggcttta tcagcaacat atggtcccat
    15601 gctatcattt aaagatcgtc ctgaatctat catttctgca tgatacccta actgtttggc
    15661 tttatgtaac aaataataag gatctactcc aatacaatgt ccaccaacta atccaggagt
    15721 aaagtttaag aaattccatt tagttttagc agcttctaac acttcaaaag tattaattcc
    15781 aattttatca aaaattattg ataattcgtt cattaaagca atgtttacat ctcgttgagt
    15841 attttcaata attttagcag cttcagcaac ttttatagag ctagttctgt gaacaccagc
    15901 tttaataatt atttcataca cttttgcaat ttcttcggca gactcggcat cgcaacctga
    15961 agtgattttt acgatatttg gaagtgtgtg ttgtttgtcg cctgggttaa ttctttctgg
    16021 agaaaagcca actttaaaat cttttttaaa ctttaatccc gacatttctt ctaaaatagg
    16081 tacacaatct tcttctgtgc aaccaggata aacagttgac tcaaaaacaa cataatctcc
    16141 tttttttaaa attttgccta ctgttttaga tgcacttaaa ataggacgta aatcaggtag
    16201 tttgtgctca tcaataggtg taggaacagc aacaatgtga aaatgacact ctcttaaatc
    16261 ctctgggttg gctgtaaata aaatatcagc attttcaaaa gcctctttat ctaactcatt
    16321 agatgggtcg attttgtttt tcatcagttc tatgcgttct ggtttaatat cataaccaac
    16381 aactttaacg aacttagcaa attcaagtgc aattggtaag ccaacatagc ctaagccaat
    16441 aactgatatt tttttctctt tttttaaaat tgatttgaac ataataaaat tcctttatta
    16501 ttatattttt aataagttta tttctttaac aatggatggt aatcaccagt aattcctatg
    16561 ggtgtagcat tcctaatatt aaatactgtt tgaattgaag gcttggcttc tttaagacca
    16621 aatcctttac cacttaaaat atgacgataa ctttctgtat gtaaatctgt aaaaccatca
    16681 gaaaattcta tttcttcgtt ttccatttta atagaacgat aagtacgttg tcctttagat
    16741 ttaatttcgt ctggcaacat gttttcatca atagaaagca cccatcttac tcttgctctt
    16801 tctaattcga gatagccagc agctttatct ttttcactta catgtactac acttgattta
    16861 acttctccaa aaatccatga aagcatatcg aagaaatgaa ttccaatatt tgttgctata
    16921 ccaccagatt tttgaatatc gcctttccac gaataatgat accattttcc acgacttgta
    16981 atgtaagcca aatcaatatc gtaaatttta tcttttggac cattttctat cttttcttta
    17041 agatttttta ttgactgatg taatcttaat tgaagaatgt tgtaaatttt tttgccagat
    17101 tctttttcaa tttcagctaa ggcatcaata ttccaagggt ttaaaaccaa aggtttttca
    17161 caaatagcat gggcttggtt tcttaaagca aatctaatat gggagtcgtg caaataattt
    17221 ggagaacaaa tacttacata gtcaattttt ttatctgtac gggatagttt gtaaacgtgc
    17281 ctgtcgaaac gctcaaattc atgaaaaaaa tctgcattag gaaaatgtgc atctaaaaag
    17341 ccaacactat caaaagggtc taaacatgca acaagttcgt ttccagtttc ttttattgct
    17401 tttacgtgtc ttattgcaat ataacctcca acaccaacta atgcgaaatt caaatttgac
    17461 atattgatta taattttttt acggaattgt taataagttg atacttctca ccactttcag
    17521 ggcaagttgc aaagccattt ttgtcgaaat ttagtttatg tccatattca ctcatccaac
    17581 cagtttgtcg agcaggattt ccaacaacta aagcatagtc aggaactgct tttgtaacta
    17641 ccgcaccagc tccaataaaa gcatatctac caatatcgtg tccgcaaact attgtggcat
    17701 ttgcaccaat acttgctcct ttttttacct tagtttttaa gtaggaatct cttctaacaa
    17761 ttgcacttct tggatttatt atatttgtaa aaaccattga aggacctaaa aaaacatcgt
    17821 cttcgcaact aactcctgtg taaatagaaa cattgttttg cacttttaca ttattgccta
    17881 aaactacatc tggcgaaacc acaacatttt gaccaatatt gcagttttct ccaattctac
    17941 aatttgacat aatatgcgaa aaatgccata ttttagtgcc ttttcctata atgcagcctt
    18001 catcaattac tgccgtttcg tgtgcgaaat aatcctgttt catatttttt acaaaaataa
    18061 ttaataatct ataaaatcga aaaaaatata aataaaagtt tttattctat aaatttgttc
    18121 gatattggat cccactcaat ttcatgactc cagtttgctc tgatttcata ttctgaaggc
    18181 aaaaaataaa ctttttctgc atgttggttt ttaatataat tcccagtgtc ccatctgccg
    18241 ttttgatttg agtctaaaat tacttttacc ttatatttcc ctgcttttaa aaattcaaaa
    18301 acaacttctt tattctcttc tgaactttca gcaactactt tttcgttttg tatcatttgc
    18361 acaagatagt ttgtattggc ttcgggaaat attattttaa aattaccata ttcactactc
    18421 gaagcagttt gaaacttcaa agtgtcaaca aggttaggtt cattataaat atttttaaga
    18481 aatgccgaat ctgctaataa tctatattca gacttttcta ataaatcgga ttttagtgct
    18541 aaaattagcg gatttatgct gtctttttct aatttaaaat ctgttttaat atattcgttt
    18601 tctgaaatta gttctaattt tagtttgctt gggtcaaatt cactaattgg aaattctgtt
    18661 ttaatttcta aatctaaaag aggctcttta gtctttggta tggaaagttt aacaatattg
    18721 tcttctaatt ttttgtcaac agatcttaaa aataaactgt cggtaacaat attgatagat
    18781 gattcaaaat caggataatt gcaaattatc tgcattcttg atgaatcaat tatttctttt
    18841 tttgttaaaa aaacttgtaa ggtgtcaaga cttttactca tttttaaaat tgcattttca
    18901 tcgcctatta cattgatttc aaaattgtcg tgttgtggtt tgttaaaaac aatttctacc
    18961 aatcttggat taattctttt gcttgatttt ataaattgag tttgattatc ttctttaaaa
    19021 attaaaagtt ctaaatttgt tggtttatta attgttttgg cagctgtagt atctgttttt
    19081 gcaattaatt taacactagg ttgtattgtt gtgtctaaaa atgcaatgct ttcttcagga
    19141 aggtcgaaga ggtaattgta attattgtcg attagtgcat aaattttata ttcgttttcc
    19201 ttgacaaaag ggattaaaaa ttttccattt tggtcagttt ttgtgatata cgaaggttga
    19261 gattttatta ttgcagagtc acttaaatca gaatataatg aaacccaagc atcttttaca
    19321 ggttttctgt cgaaagcatc taaaactaca ccgccacata acaaagagtc tatcttatct
    19381 ccagttgaaa atgcaaaaac aaaagaatta agcacattat tttcattatt atcggcaatg
    19441 gcatcattaa aatttaaagt gtaagttgtg ttttcttcta actcattaga ttttagcttt
    19501 attaaaactc cttttctttt aattgtaact acaggttttg ttggcattgg tggtgaaaca
    19561 attaattttt gatttacatt atttaacctg atatattcat caaattgaat aagaattttg
    19621 tcagaattaa aatttgttga gccgttttgg ggttctgatt ttacaaattt tggtgggctg
    19681 gtgtctttag gtccaccaga aattgctatt tgcttggcac aagaagcaat tgttaaaata
    19741 atgtatattg caaaaataaa tgatattaaa ttaaatcttt tcattaaaag caatttgatt
    19801 taaaatgcaa atttattatt aaaatttaat atttgttgta aaaccatttt aaaatactat
    19861 ttccaaaagt ttcagtttta aattccaatt tttcaaaaat aggagcaggc acgccttttt
    19921 caaaaatatg atcgccaaca aacacccttg cttcgtccca tatattcaaa tcaataaatg
    19981 attgtaaggt ttgtgtgcca ccttcaacaa caagcgacaa aatgttaaaa tcatataaat
    20041 tttctaaaat ttgaggtatt atatttttag aaaaatcaat tttcaagtaa gtaatattgt
    20101 tgataatcaa ggttgtgtgg gagttgaaaa taattgtttc ggcttcttta ttaaaaatat
    20161 tgaaatcttt agataatgtt agatttttat caatgcttat gcgtgttgga tttttccctt
    20221 tccacagcct gctagtaagt tgtggattgt ctaataaaac tgtatttgtg ccaactaaaa
    20281 acgcatcttc ttctgttcgc catttatgaa caagagtttt acattcatca tttgttatcc
    20341 aaaatgcttt ggctttgtcg gaaggcgttc ttacaaaatc aatatagcca tcttttgttt
    20401 ctgcccattt taaaattata taaggtcgct ttttttgatg aaaagtaaaa aatcttttat
    20461 tcaaatcaat acatttgttt tcgagtacac ctgtaattat ttctctgtta gcagttttaa
    20521 gaatttcaat tccttttcca gcgacttttt catgtgggtc gatagtgcca attacaattt
    20581 ttggaatatt tttttctact atcaaatttg cacaagcagg agtttttcca acatgagaac
    20641 aaggctctaa agaaacataa atagttgact tgcttaataa agatttatct cttacagaat
    20701 taatcgcatt tacttcagca tgagcttcac catattttct gtgatagcct tctccaataa
    20761 tcttgccctc gtgtactata acacttccta ccattgggtt gggcgaaact gagcctaaac
    20821 ctttttgagc aaggtcaagg catctttgca taaatatttc atcaatacta ttcatagttg
    20881 caaagatatg gtttagaaat taaaaaagat aattttttgt tatttttaaa aatttaaact
    20941 ctaagtataa atacgtattt ttttaaactt gttgatataa ttattgtcaa aaaagtttaa
    21001 aatggtagtt atttttctat ttttgcaact tattaataat aattatttag tattatgaga
    21061 aaagttttac ttattgtttt tattattgtt accacttctt ttgctctgtt ttcacaaact
    21121 gttttattat ctggaaaagt tgttgatgcc gaaaataatg aaggactttt tggagtaaat
    21181 attacaatta atcatgctga agagttgctt gctaataagc aacaattagg gacttcaaca
    21241 gattttgacg gcaattataa aattgcatta tatcctggcg attatattgt taaatttgct
    21301 tttatcggat ttgaagaagt aacaaaagaa gtagaattac ttgttgggaa accagtagtt
    21361 ttagatataa atttatcgcc tacagcagaa attattgatg aagtagttgt gagtgctggt
    21421 aagtttgaac aaaaactatc agacgttacc gtttctatgg aagttttaaa accaaaaatg
    21481 atagaaagta ataacaccac tcgtttagac catgctctta atatggttcc tggtgttgac
    21541 gttaatgatg gtcaggcaag tattagagca agtacaggct ggagttatgg agcaggaagt
    21601 cgtgtaattg ttttaatgga cgatttacct ataatgtcag ctgatgttgg agatgtgaaa
    21661 tggtattatg tacctttaga aaatgtgtct caagttgaag ttgtgaaagg tgcaagttca
    21721 gcattatttg gctcatcagc tcttggagga gttataaatg taagatctgc atatcccaca
    21781 aacaaacctg ttactaaagt gtcttacttt aatggtattt atggaaatcc tagagatttt
    21841 cgccaaaaat ggtggggaaa agaattcttt gcaaataaca actctccttt aaaaattcct
    21901 ttgcgtgaag agctttttta ttttgtgcga aatcctatgt atgcgggttt aagttttcat
    21961 catgctcaac aatttgacaa tttagattta tcattaggtg gaaatcatca tatcgatgaa
    22021 ggatatagag aagctgaata cgaacgccgt agccgattta atgcaaattt aagatatagg
    22081 gctaaaaaag ttcaaggatt ggctgtagga gtaaatacaa actttatggc ggttgataaa
    22141 agcgactttt ttatgtggaa agcagatact aatgcttacc aagcaaatcc aggattagga
    22201 acaactgcac caacacaagg ttttagaatg aatctagacc cttatgttaa ttattataga
    22261 ccaaatggag ataaatttag tttgagaaca cgctattttt taaccgaaaa tgttattcct
    22321 tcagatacaa tgaaaaactc taaaggatac acttattatg gagagtttca atatcaaaca
    22381 gtacttgctg aaaaatggaa tttagcagca ggaattgtaa attcatactc aaatgtttac
    22441 tccaatatgt ttggcgacca ttttagcaat aatttatctc tatatacaca tgttgacgtt
    22501 acttttggaa gattgaaact ttcgggtgga ttgagaggag agtactttgt tttggattcc
    22561 actcaaaccg aatctttgtt tgatataaaa cttggtaagg aacgcttaga aatcccattt
    22621 agaccagtat ttagaacagg ggtaaactat tcggttacag accaaacttt tatacgggct
    22681 tcatttggac aaggttatcg ttttccgtca atagcagaaa aatatactgc aacatcatta
    22741 ggaatggtaa atgttttgcc taatccaaga ttgttgccag aaacaggctg gagtacagaa
    22801 ttaggaataa agcaaggata taaatttggt aaatggcgag gttttgccga cttagcttta
    22861 tttaatcaag atataaataa tatgatggaa tttaccttcg gtgtgtttaa tccagtaaca
    22921 tatatgcctt tccaacctac agatactgtt attccagtat ttgcttttca atcacaaaat
    22981 tatggagatg ttagaattac aggagcagat ttgtcggcaa gtattgctgg taaagtgggc
    23041 aattatatga ttatgggaat ggtgggatat acttatactt atccagtacc aaaatttgga
    23101 gcagacacta cagcttctag tttatcgcca attttaaaat atagaaacag acattctata
    23161 aaagcagata taggagtaga aactaaacgt attacttttg gtgcaaatat tatttggcgt
    23221 agtgctatgg aaaatgttga cagactgttc tgcgacgaaa gagatcccgc aacggtaaat
    23281 cttactgata tagcttttta tcaatttttt agccaaacaa ttttaccagg atattggaat
    23341 tatagaattg aaaatgcaaa aaagaattat tttaatattg acctaaggtt tggatttaat
    23401 ttcaataaat atttaagtgc taattttatt gtaaaaaatc tattaaatgc agaatatgtc
    23461 ggaagacctg gagatatgca tgctccacgt agatacgaaa tattaataag tgctcatttt
    23521 taattattgt aaaactttta atttaaaata ttatgaaaca tgtaattttt actttttttg
    23581 ttgttttatt ttctttagca ttaaatgctc aaatttgtga tcctaattgt actcccgacc
    23641 aatcttgtgt cgaaaatttt gaaggtaatg gaatgatatg tcctgtagat cttcctgttg
    23701 ctacacaaaa tgttttttat gacgaaacag ttactgttat tccacctaaa aattttgaag
    23761 gtctttcttt gattggtgct attagaattg atgaggttaa tggattgcca gaaggaatag
    23821 cttggtgtaa aagtcaagaa atatttaatg ttacaactcc gcttactcgc tattgtgcac
    23881 aattaaaggg aactcctaca acacctggcg agtatcaatt aacattaaaa attactcctt
    23941 atcttaactt ttttggtaca ctcgtggaac aaagccctgt ctatgatgac acttctttac
    24001 taatagtagt tcttccttat gttgagcctc ctgtagcaga ttttgaagct aatgtaacaa
    24061 ctattgagca aggtcaacaa gttagtttta ctgatttaag cacaaatgat ccaacaagtt
    24121 ggtcttggat ttttgaagga ggaactcctg gtgcaagtac agaacaaaat cctacagtta
    24181 cttatatggt accaggaact tataatgttt ctcttaccgt agctaacgca ggtggtgagg
    24241 ataatgttgt aaaaaatgca tatattacag ttactgcaac acctacacct ccagtagcaa
    24301 attttgttgg aaatcctaca actattacag ttggcggaac agttgctttt acagaccaat
    24361 ctacaaatac ccctacatct tgggaatggg tttttgatgg aggagaacct gctacaagca
    24421 cagatcaaaa cccaacagta acctacaaca cagcaggaac atacactgta aaactaacag
    24481 caacaaatgt tgatggtagc gatataaaaa caagaacaaa ctatattaca gttgtggaag
    24541 atggttctct aacagcagac tttattggaa acccaacaac tgtagaagaa ggcgacactg
    24601 tttactttac agaccaatct ttgggtaatg taacttcttg gaattgggaa ttttctggag
    24661 gtaatccagc aacaagcaca gaacaaagcc catctgtagt atatgaaatt gctggcgttt
    24721 atgatgttaa gctaacaatt agcgatggaa caaatcaaga tgaagaagtt aaagttgaat
    24781 atgttacagt tattccacaa ccagaacctg tagttgctga ctttaccgca aatcctacaa
    24841 gtgttgaaat tggcggaaca gttaatttta ctgaccaatc cactggtggt gttacttctt
    24901 ggaattggca attcccaggc ggtaatccag caacaagtat tttaccaaac ccatctgtag
    24961 tttataatac tgtcggcact tatgatgtta ctctaacagt tggcaatggt acaagccaag
    25021 acgaagaagt taaaactgct tatattgcag ttatcccatc aggaattgaa ggagtagatt
    25081 ttgattttgt aaaaatttat cctaacccag cgtctgataa tattgttgtt gaagcactag
    25141 gcttagaagc cgtaagcatt attgatgttt tgggaaaagt agtttataca aaaacaacaa
    25201 attcagacaa agaatttatt gatgttagca aattgcaaaa agctaattat tttataaaag
    25261 taaaaactaa tagtggcgaa attgtgaaag ctattagtgt gaaataaata ataggttttg
    25321 ttatattata aaagctctat ccgaaagaat agagcttttt ttattctttg ggatattcag
    25381 aaattgttaa taaaaagaca acaaattgat tgttaattag ttat
//
LOCUS       DAOPNX010000028        25363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_2482_length_25363_cov_6.188755, whole genome shotgun sequence.
ACCESSION   DAOPNX010000028 DAOPNX010000000
VERSION     DAOPNX010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25363
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2482_length_25363_cov_6.188755"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..543
                     /locus_tag="PKN53_04605"
     CDS             64..543
                     /locus_tag="PKN53_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88268.1"
                     /translation="MTSCISIKTVVDESAIKEPYKNPLIVILYDKYNTKKFSEKLKTN
                     MELQFRNANIKVEFIFSDLKSSDELTLNQNDDLNTKINNAINNDQKDIVFILKPASIS
                     YQNNAKFVNYSLTGIDVVTQKEVWKARFNTSSSFETNGLAKKAAQKILERLKTDKVL"
     gene            complement(720..1697)
                     /locus_tag="PKN53_04610"
     CDS             complement(720..1697)
                     /locus_tag="PKN53_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HOD88269.1"
                     /translation="MEAKGTISKFIEENFKHFNAATLVDASKGYVKHLTEGGKMMITL
                     AGAMSTAELGKTLAEMIRQDKVHIISCTGANLEEDIMNLVAHKHYKRVPHYRDLTPQE
                     EWDLMQKGLNRVTDTCIPEEEAFRRIQEHIFKQWKTAQDKGERYFPHQYMYKMLLSGD
                     LEKYYEIDPKNSWMLAAAEKNLDIVVPGWEDSTMGNIFTSYCIKGDLKPSTMKTGIEY
                     MMFLTDWYRKNSSGKGVGFFQIGGGISGDFPICVVPMMYQDLEWEDVPFWAYFCQISD
                     STTSYGSYSGAIPNEKITWGKLDINTPKYVIESDATIVAPLVFAWVLGY"
     gene            complement(1697..2548)
                     /gene="speB"
                     /locus_tag="PKN53_04615"
     CDS             complement(1697..2548)
                     /gene="speB"
                     /locus_tag="PKN53_04615"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOD88270.1"
                     /translation="MSKRTYAGIPEEYSDYENAKVVILPVPYDKTSTWGKGADKGPEA
                     FLDASENMEIYDLESDTEVFKHGVWLSDVLDVDDNPEMMVQQVKAKAKKFYDDNKLFT
                     MIGGEHSVSIGAIMASAEKFENLSVLQLDAHSDLRPEYDDSIYNHACALHWASKNTNL
                     IQVGIRSMAVEEREFVNKNNVFYAEDIANRNDWQQEVIDKLGQNVYLTIDLDYFDPSI
                     LPATGTPEPGGLTWYPTLDLLKKLAKQRKIVGFDIVELAPDPAHLASNFLAAKLYYKI
                     LTYILEK"
     gene            complement(2581..3978)
                     /locus_tag="PKN53_04620"
     CDS             complement(2581..3978)
                     /locus_tag="PKN53_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HOD88271.1"
                     /translation="MKTKYIDLIEQSFEFPTEEFEVEDGELYFYDIPLMDLVKQYGTP
                     MKFTYLPKISENIKKARVWFNVALTKANYNGKYYQCYCTKSSHFSFVLEEALKNDIHI
                     ETSSNFDIDIVRALYESGQIDKNKFIVCNGFKMPAYIENIARLINDGFTSVIPVLDNY
                     SELDQLLEQVEGEFSVGIRIASEEEPKFEFYTSRLGIGYKNIVQYYHDFIENNPRVKL
                     KMLHFFINTGIKDTAYYWNELNKCMNVYCELKKVCPELTALNIGGGFPIKNSLTFDYD
                     YEYMAEEIVNQIKMACDANKVPEPDIFTEFGSFTVGESGGAIYSVVFQKQQNDREKWN
                     MIDSSFITTLPDSWSISKRFIMLPINKWESQYERVFLGGLTCDSDDYYNSEQHVNAIY
                     LPEYDEEETLYIGFFNTGAYQEAIGGFGGIQHCLIPAPKHVLISKDEDGKVTTRLYSK
                     EQSYKSMLKTLGYTY"
     gene            complement(4248..4838)
                     /locus_tag="PKN53_04625"
     CDS             complement(4248..4838)
                     /locus_tag="PKN53_04625"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOD88272.1"
                     /translation="MEYLENLDIDIFLYFNSLNLAWLDPIMKFISSFWIWYIVVAIFI
                     FLLVRKFKKKFWIPLLVSIICFALTDQSSNLIKHSVKRYRPTHNLEISDKVHTIDGYK
                     GGLYSFVSGHACNGFGLALITLLFLRNKKWTWFILIWASIVSYSRIYLGVHYPADLVA
                     GAILGTCLALAVYKIHKLIPYFKNLETDRKMASPNV"
     gene            complement(4838..5830)
                     /gene="obgE"
                     /locus_tag="PKN53_04630"
     CDS             complement(4838..5830)
                     /gene="obgE"
                     /locus_tag="PKN53_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOD88273.1"
                     /translation="MVTSNFVDYVKIFCRSGSGGAGATHFRREKFVERGGPDGGNGGR
                     GGHIILRASTHRWTLLHLKFQRHIFAENGESGGASNRTGADGADQIIEVPMGTIAKDA
                     ETGEFLCELTEDNQEIILLEGGKGGLGNTEFKTSTNQAPRYSQPGIEGIEKAIILELK
                     LLADVGLVGFPNAGKSTLLSVVSAAKPKIADYPFTTLVPNLGIVEYRDSKSFVMADIP
                     GIIEGAAQGKGLGHRFLRHIERNSILLFMIPCDTKNIKKEYEILLKELREYNPELKDK
                     TRILAITKSDLIDDELMELLKAELPKNIETIFISAVANKNIDKLKDLIWNKLNE"
     gene            complement(5836..6414)
                     /locus_tag="PKN53_04635"
     CDS             complement(5836..6414)
                     /locus_tag="PKN53_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOD88274.1"
                     /translation="MKIAILAGPPGSGKSTHSKLLAEKLGFVHISTGNIFRKEIEKNT
                     DLGKLAQSLIDKGNFVPDEIVCEMIEKIITTSDENTKFVFDGFPRNYNQAVEFEKILE
                     KLNLKVNVFLEFNVCEDSLISRLIERSKSSNRKDDSDVRIIKHRLEVYKNNTHIINDF
                     YFSRTNCFKINGDRPISVVSAEIFEIVNKFMS"
     gene            complement(6710..7189)
                     /gene="nuoE"
                     /locus_tag="PKN53_04640"
     CDS             complement(6710..7189)
                     /gene="nuoE"
                     /locus_tag="PKN53_04640"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOD88275.1"
                     /translation="MSRIQIKLKEQDYKAIREICHSFNNDPEELINVLHKTQGHFGYL
                     PAEVQEAVAQELNVPVAKVYGVVTFYSFFTMSPKGQFPISICMGTACYVRGAEKVLEE
                     VKKELKINVGETTPDGKFSLSCLRCVGACGLAPVVMIGDKTFGRVAPEDVKKILAEF"
     gene            complement(7197..8969)
                     /locus_tag="PKN53_04645"
     CDS             complement(7197..8969)
                     /locus_tag="PKN53_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOD88276.1"
                     /translation="MSLINIKINGKDIQVAAGTTVLQAAKKVGIEIPTLCHMNLHDLN
                     IEHNPGGCRICVVEVAGRRNLAPACVTKVTEGMEVKTHTMRVLNARKTVLELILSDHP
                     FECLTCAKNGTCDLQKLAQDLGVREIHYQGEQSSYKEDNSPSIIRDMDKCIMCRRCET
                     MCNEVQTCGVLSGVNRGFDAVVAPAFEQDLIQSPCTFCGQCVAVCPTGALTERDYTNR
                     VIRALADPTKTVIVQTAPAVRAALGEEFGLEPGTLVTGKMVAALRKIGFDKVYDTDFA
                     ADLTIMEEGTELLGRLTKFLNGDKDVKLPLLTSCCPAWVNFLEYQFPELKDIPSTAKS
                     PQQMFGAVAKTYIAEKLGVAREDLIVVSIMPCLAKKYEAQRPEFAVNGNPDVDFALST
                     RELAHLIKQSIGDLKDLEEEDYDKLMGQSTGAAVIFGNTGGVIEAACRTAYEVHTGKT
                     LEKVDFKQLRGLEGVRTASVDIDGLELKLGIAHGLGNARKLLEMVKNGEADFHAIEIM
                     ACPGGCIGGGGQPLHHGDSSILKARAAALYRADEEMKLRKSHENPEIISLYKEFLGQP
                     MSEKAHHLLHTEYFDKKKVIYIEK"
     gene            complement(8984..10777)
                     /gene="nuoF"
                     /locus_tag="PKN53_04650"
     CDS             complement(8984..10777)
                     /gene="nuoF"
                     /locus_tag="PKN53_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOD88277.1"
                     /translation="MAKYKMHILVCGGTGCKSADSAKIVDSLNAELESHGIESEAKVI
                     LTGCFGFCEQGPIVKVIPDNTFYTQVKPSDAKEIIEEHIIKGRKVTRLLYTNPENKEH
                     VSDSKHMDFYKKQIRIALRNCGFLDPENIDESLARDAYQALGKAITEMTPQEVIDEIK
                     KSGMRGRGGAGFPTGLKWEIAAKNKADQKYVVCNADEGDPGAFMDRSILEGDPHTVLE
                     AMAICGYCIGADKGLIYIRAEYPLAIKRLQIAIKQAKEYGLLGKDILGSGFNFDIELR
                     YGAGAFVCGEETALIHSMEGERGEPTVKPPFPAESGFLGKPTNVNNVETFASIPVILL
                     KGAEWFSSIGTEKSKGTKVFALAGKINNVGLIEVPMGITLREVIFEIGGGIKNDKKFK
                     AVQTGGPSGGCLSEKHLDTPIDFDNLLAAGSMMGSGGMIVMDEDDCMISIAKFFLEFT
                     VEESCGKCSPCRIGNKRLHEILTKITEGKGTLEDLELLKNLSGVIKDTALCGLGQTSP
                     NPVLATLNNFWDEYVEHVTEKKCRAGQCVALKQYVIDPEACTGCTACARVCPVGAISG
                     ELKKPHTINQETCIKCGACYEKCKFNAIFIK"
     gene            complement(10792..11184)
                     /locus_tag="PKN53_04655"
     CDS             complement(10792..11184)
                     /locus_tag="PKN53_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOD88278.1"
                     /translation="MNKIKNLADLRKMKDDLHNKITLRERSDSPEAIIQIRVGMATSG
                     IASGAKEIMAYLIEELDKRNIEALVLQVGDMGYCYAEPTIEVTRPGEDPVVFGYVDIA
                     KADEIIEKYIKNGEMIEGILPANYQSID"
     gene            complement(11538..12068)
                     /locus_tag="PKN53_04660"
     CDS             complement(11538..12068)
                     /locus_tag="PKN53_04660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD88279.1"
                     /translation="MKTIDLHIIDIANNSIRAKASEILIEVKDSEKDNSITLVITDTG
                     CGISPEKLKEINESFYSSRKTRKIGMGLALLKYHAEITGGKFELTSELGVGTKVYAKF
                     VRNHIDRQPIGDLPGTIAMFICQYSDINFIFRYISDDNEFELSTADVKSVFENIELNN
                     HLILSNIKELIANSIE"
     gene            complement(12071..12793)
                     /locus_tag="PKN53_04665"
     CDS             complement(12071..12793)
                     /locus_tag="PKN53_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOD88280.1"
                     /translation="MIFKADLHIHSVLSPCGDLEMSPSAIIAEAKSKGLNIIGISDHN
                     STRNALVASKIGQKNDVFVLCGAEVTTKEEVHCLCFMPDENTLLEFQNFLDSKYIFFQ
                     NDPDKFGYQLVVDEDENIVDQIDTLLINAIDCGIEDLQKKVYELNGIFIPAHVDKPRF
                     SLSSQLVFIPENLKFDALELSKYAEKNNFLQNFSWFSLNRFITSSDAHYIPDIGTVYT
                     ELEMETPSFENIRSALKNIKPR"
     gene            complement(12790..13119)
                     /locus_tag="PKN53_04670"
     CDS             complement(12790..13119)
                     /locus_tag="PKN53_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HOD88281.1"
                     /translation="MLLKDIIEKLELKVLNLANENVDVKDAYASDLLSDVMGKSKAGQ
                     IWITLQTHKNVSAICSLKDLAAVIIIGNGQPDPDMLEHAKEEEVCVLSTPDSCFNICG
                     KLYELIK"
     gene            complement(13123..14457)
                     /locus_tag="PKN53_04675"
     CDS             complement(13123..14457)
                     /locus_tag="PKN53_04675"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOD88282.1"
                     /translation="MEEPKGTYFYHALKVDHDICVGCTHCLQACPTEAIRIKKGKAVL
                     YANKCVDCGECFRVCPVRAIYIKQDDFAELGKYKYRIALVPAVFISQFAENVRTSQVY
                     ACLKKLGFTHIYEVEHGVGVLIELMNNYMRKNQDRKPFISGYCPAVVRLIQVRFPSLV
                     QNIIHLKAPLDVASITILERLTDAGISKDEIGIFYITPCAAKIAAVKSPVENVSSSIN
                     GVINIDFLYNKVQQMLAKENTPAEPINHSLSGRDILWSTSGGEAQNFAGNSFAVDGIR
                     NVIGFLERIENDEIESDGIIEMRVCNQSCAGGVLNPNNRFVAVNKQKRRAESLDNQLD
                     GRSCFLPVFSQDFNNRVKELMTIDEIKPRSILKLDDNLQKALKMVESINKTKAILPGI
                     DCGACGAPTCSALAEDITRGNAEIEDCIFMSAECDKSKETLGRIWNYKNKNK"
     gene            complement(14461..14874)
                     /locus_tag="PKN53_04680"
     CDS             complement(14461..14874)
                     /locus_tag="PKN53_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HOD88283.1"
                     /translation="MELNFKIEGGNFSGAGSASSEVKKTLKQLGVDASQIKRIVIALY
                     EAEINVVAHAYRGQIGVKISADKIFARIEDEGPGIADISKAMQEGYSTASSKVREMGF
                     GAGMGLPNIKSNVDEFDIQSVVDKGTVLEMTFYLA"
     gene            complement(14879..15229)
                     /locus_tag="PKN53_04685"
     CDS             complement(14879..15229)
                     /locus_tag="PKN53_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88284.1"
                     /translation="MKVADIKELVEGRVVNNKVCLENIGEIKHAFASDLMSDVLRLKC
                     ENTLLITGLCNVQTIRTVEMAEIKLIVFARGKKVDENMLELAEENDICIIETDFSVYK
                     VCGLLYQNGISPVY"
     gene            15540..16082
                     /locus_tag="PKN53_04690"
     CDS             15540..16082
                     /locus_tag="PKN53_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88285.1"
                     /translation="MQSSQSLEDFKNKMGNLNELCANALVKDETLMEKAFYEKHKENV
                     RILDAERILQKVDSCSNATDLGIMITEETNKNSMEITSDEAHRVLVDDWFLLDKLEIY
                     ENAEVPSEYKSEMKQIASDIRNSIIENARSVEEDMQAWENRWRLKPEILLEYRHKRLF
                     PYEDKHIEEQIARYKSIINR"
     gene            16095..16271
                     /locus_tag="PKN53_04695"
     CDS             16095..16271
                     /locus_tag="PKN53_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88286.1"
                     /translation="MAIDRMMLDPTLDTYRKMLKDLQEQNITGEDMDKMAEIIARMEQ
                     LGNELSDINDFLGK"
     gene            16271..17512
                     /locus_tag="PKN53_04700"
     CDS             16271..17512
                     /locus_tag="PKN53_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88287.1"
                     /translation="MQEDLFGKFSAHYTKALTSQYQAQNSENGGTYNDAALLKQCVDA
                     LKYAVTTIKDSYNKTIEDAKNFDAKEHKDKSIEYFEETTGTTVGKFFKKQAKKDLDKT
                     LKEKPNAFDNSIEVAVLHDPELIIKGIQDLIDLGEQEGMTTPKFLRLQIETGLDKAMQ
                     GTSTFRKALEFQLDSTLANPTPWTLKLAEEKLRVFDELAAKNKFNIPNLKELELAHND
                     IDYIYERDIKIWDEIIERWKDLLGDLDVWSLSHCSFAPSIEPWRMARDPKQATIKTQK
                     TTPGIFKQKEKLLKKYFGLNFMDVFTHHSFEWNVKYNYIEYSQEFTEFLIEKVYPQCV
                     PLNSLNSDIINERASFYPTGSNPDRETNPHCNMYAKRLRDFYDSKFGKGRYDSKFGVI
                     NEINSAAKPWDWDSFKFKNKI"
     gene            18252..19760
                     /locus_tag="PKN53_04705"
     CDS             18252..19760
                     /locus_tag="PKN53_04705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOD88288.1"
                     /translation="MKKRKMVNGIIVLMSISLILIIIMQATHLSKSYKSSKEIVERGI
                     AEAISRSIVVLQKQDAVFFVYDKLHQKNKVVDSLVPIDPYLVQLGLRPIFNQYSNIHF
                     QSFPGTGLSSVSFHSEFSDLSYIESYMFQQFTQKTIEFDQIINQLETEFLQRQIPIEQ
                     RFDAETVEITLKKSLASVGLNLDFEFAISDNDQNIKLKSSNFNAKNLKNCYKFNLTPA
                     SVFTNPDVLLIDFPTKDKYVLETIYAQLGTSIVLILIFVLTFSTSLYALIKQKKNAEI
                     KNDFINNMTHEFKTPIATIKLAAASIQSNNAKENPEFVNNMLNIINQETNRMNSHVEQ
                     VLQMAVLDKKNVQLKISTENINELVAEVAKNIELVILEKNGELKLNLCEENPNLRIDR
                     DLIANALDNLLDNAIKYSKTSPEITVNTYIKNDKFNISVEDKGIGMSKDVLNKIFERF
                     YRAHTGNLHNIKGFGLGLNYANEIVNAHNGHINVKSTLGKGSTFIITLPMIN"
     gene            19773..20486
                     /locus_tag="PKN53_04710"
     CDS             19773..20486
                     /locus_tag="PKN53_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOD88289.1"
                     /translation="MNKQNNNKQKLLLVEDDVNFGFVLKSYLELNEFDVDLVSDGKDA
                     FPTFNKNTYNLCILDVMLPNVDGFTIAKDIRKINKNIPIIFLTAKSQKDDVLEGFKSG
                     ADDYLTKPFDSEVLLAKIRAIIRRNSNRPGQSLDKKINIGRYVFNTQLRTLQDGDTVH
                     KLTPRESDLLQELYLYRNNVLDRNQALKKIWGDDNYFNARSMDVYITKLRKYFKDSDT
                     VQINNIHGSGFVLILKDEQ"
     gene            20549..20818
                     /gene="yidD"
                     /locus_tag="PKN53_04715"
     CDS             20549..20818
                     /gene="yidD"
                     /locus_tag="PKN53_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004344733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOD88290.1"
                     /translation="MKFVKILKYILILPFLILVKLYQWLISPLIPPSCRFTPTCSNYS
                     LEAFKKHGPIKGFILSTWRILRCNPWGGHGHDPVPEKFYIFKRKK"
     gene            20828..21154
                     /locus_tag="PKN53_04720"
     CDS             20828..21154
                     /locus_tag="PKN53_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005780971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HOD88291.1"
                     /translation="MDWIILFIAGLFETGFAFCLGKAKETNGTEMYLWYFAFLVSLTI
                     SMLLLIKATKTLPIGSAYAVWTGIGAVGTALIGIFVFKEPTNFWRLFFIFTLIASIIG
                     LKIASN"
     gene            complement(21151..22047)
                     /locus_tag="PKN53_04725"
     CDS             complement(21151..22047)
                     /locus_tag="PKN53_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOD88292.1"
                     /translation="MKFLNKEKLFSAKWFKSLALILSGTFIMSLGYVYFISPYKITPG
                     GVYGIAIVIHHLFDLPIGLTALAFEIPITLLGLKLLGPKFGWKTVVGFVSMAGFVDLL
                     TYFNGDVPLVKDDTLLSAIFGGVLIGVGLGLVFKSRASSGGSDVIAMILNKYTRIPLG
                     TLVIYVDSAIVLISLVAFKDWTIPLYSWLVIYITGKAVGIILEGASYEKAIFIISDKY
                     EEIKETIIQDLNRGGTIFHGKGMYSQEEKQIIYTTVSRREFELLKDYIYEIDPKAFIT
                     VTEAHEIIGEGFKSMRDSLSSD"
     gene            complement(22168..23445)
                     /locus_tag="PKN53_04730"
     CDS             complement(22168..23445)
                     /locus_tag="PKN53_04730"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOD88293.1"
                     /translation="MKIDVLLGLQWGDEGKGKIVDVLTPKYDIIARFQGGPNAGHTLE
                     FNGIKHVLHTIPSGVFRKNAINIIGNGVVIDPIILKKEIEDTEKLGVDLSEIFYISRK
                     AQLILPTHKLLDKAYEEYKGKNKIGSTLKGIGPAYTDKTARLALKIGDIFTESFKEKY
                     NTAKDHHQKIFKGYNFNEDYSKLEKDWFDAVEFLKKFKFIDSEIFINKSINENKSVLA
                     EGAQGTLLDVDFGSYPFVTSSNTVSSGVCTGLGVSPQKIGKVFGIVKAYCTRVGSGPF
                     PTELFDEVGQYMRDKGHEYGATTGRPRRCGWLDMVALKYSIMINGVTDLIVTKSDVLS
                     GLDTIKVATKYKYKGEIIDYMPYDMCDDIEPIYEELPGWKEDITKCTKLEELPKELLD
                     YIKYLEEKTQVKIKYLSLGPDREQTIELSVKSL"
     gene            complement(23455..23919)
                     /locus_tag="PKN53_04735"
     CDS             complement(23455..23919)
                     /locus_tag="PKN53_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOD88294.1"
                     /translation="MKAVEYNIPVRQILTDYLERKGYRKTPERFAILDEIYSRNGHFD
                     IESLHISMKNKNYRVSRATLYNNIDILLDANLIVKHQFGKNISTFEKAYACMQHDHMI
                     CTDCGKVIEFCDPRIAEIEKTISKASDFKVLNHSLYLYGICNECQKKEEYKK"
     gene            24053..25300
                     /locus_tag="PKN53_04740"
     CDS             24053..25300
                     /locus_tag="PKN53_04740"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD88295.1"
                     /translation="MIFFRLILDSIKFSFSSLINNKLRTFLSLLGITIGIFSIISVMA
                     TIDSLERGIKNNIGQLGSNTVYIQKWPWIFEADFPWWKYINRPSPKLSELTQIRELST
                     CADKIAFAASTLQTIEYENKSLDDAGVVLSTHDYMQINFMNIEKGRYFSQMESHTGQN
                     VAILGAQCAESLFENTDPIGKQIKLNGKKLTVIGVFEKQGENMFSSGSDELVLIPINF
                     GKTIFDIENDDLNPFIIVSAKEGITSGELIDELTIIMRTIRKLKPLEEDNFSLNQASM
                     ITKSLDKIFKILDLAGIIIGGFAILVGGFGIANIMFVSVKERTKEIGILKAIGAKSYL
                     ILIQFLTEATALSLVGGILGLILVWLLTAVARSLTPLDLALSFGNIMSGTLISICVGI
                     VSGFAPARKAAKLDPIKAINQGT"
BASE COUNT         8654 a         4343 c         3544 g         8822 t
ORIGIN      
        1 aaccaaaaca ttattaaagc agatgaaaat atctattacc taaaaattta gggttcgagc
       61 ttaatgacaa gttgtatatc tataaaaact gttgttgatg aatcagctat taaggaacca
      121 tataaaaacc ctctaattgt aattttatac gataaatata atactaaaaa attttctgaa
      181 aaactaaaaa ctaatatgga attacaattt agaaatgcaa atataaaagt tgaatttatt
      241 ttttctgatt taaaaagtag tgatgagcta accctcaatc aaaatgatga tttaaataca
      301 aagattaaca atgctattaa taatgatcaa aaagacattg tttttatttt aaagccagct
      361 tctatatcat atcaaaataa tgctaaattt gttaattata gtttaactgg aattgatgtt
      421 gttacacaaa aagaagtttg gaaggcaagg tttaacacaa gtagttcatt tgaaacaaac
      481 ggattggcaa aaaaagctgc acagaaaatt ttagaaagat tgaaaacaga taaagtatta
      541 taagtaactt taagcctgta actggcagtt aaaagataaa aatccagaaa ttttcacaac
      601 attccacttt acaaactttt gctttcagcg agctcgcaag ctcggttcaa actttacaaa
      661 ctttcgactt ttaacacttc aactacgttc agtgcatcgc ttttaacttt taactgagtt
      721 taatacccta acacccatgc aaacacaagt ggtgcaacaa tagtagcatc agattctatt
      781 acgtattttg gagtatttat atcaagtttt ccccaagtaa ttttttcgtt tggaattgca
      841 cctgaatatg agccgtatga agttgttgag tcgctaattt ggcagaaata agcccagaaa
      901 ggaacatctt cccattctag atcttggtac atcattggta caacgcaaat tgggaagtca
      961 ccagaaattc caccaccaat ttggaaaaat cctacgcctt taccactaga atttttgcga
     1021 taccaatctg ttaaaaacat catatactca atacctgttt tcattgttga aggttttaaa
     1081 tcgcctttta tacaatatga ggtaaaaata tttcccatag ttgagtcttc ccaacctggt
     1141 acaacaatat ctaagttttt ctcagctgca gcaagcatcc aagagttttt tgggtctatt
     1201 tcgtaatatt tttctaaatc tccactcaac aacattttat acatatattg atgtgggaaa
     1261 tatctttcgc ctttatcttg agctgttttc cattgtttaa aaatatgctc ttgaatacga
     1321 cgaaaagctt cttcttctgg aatacaagta tcggtaactc tattcaagcc tttttgcatt
     1381 aaatcccact cttcttgtgg tgttaaatca cgataatgag gcactcgctt atagtgctta
     1441 tgagcaacta aattcataat atcttcctca agatttgctc ctgtacaaga aataatatgc
     1501 actttatctt ggcgaatcat ttctgctaaa gttttgccaa gctctgctgt actcatagct
     1561 ccagctaaag taatcatcat cttgccacct tcggttaagt gttttacata gccttttgag
     1621 gcatcaacta aagtagctgc attaaaatgt ttaaaatttt cttcaataaa ttttgaaatt
     1681 gttccttttg cttccattat ttttctaata tataagttaa tattttgtaa tacaattttg
     1741 ctgctaaaaa attcgatgct aaatgtgctg ggtctggtgc taactcaaca atatcaaatc
     1801 caacaatctt tctttgttta gcaagttttt ttagtaaatc taaagtcgga taccaagtta
     1861 aacctcctgg ttctggcgtt ccggttgcag gcagtatcga agggtcgaag taatccaaat
     1921 caatagttaa gtaaacattt tgtccaagtt tatcaattac ttcttgttgc caatcatttc
     1981 tatttgcaat atcttcggca taaaatacat tgtttttgtt tacaaattct ctttcttcta
     2041 cagccatgct tctaattcca acttgaatta aatttgtatt tttcgaagcc caatgcaaag
     2101 cgcaagcatg attataaata ctgtcgtcat attctggtct taaatctgaa tgtgcatcga
     2161 gttgtaaaac tgataaattt tcaaattttt cagcacttgc cattattgca ccaatgctta
     2221 cagaatgctc accgccaatc attgtaaaaa gtttattatc atcataaaac tttttagctt
     2281 tagctttcac ttgctgcacc atcatttcag gattgtcgtc aacatctaaa acatcgctta
     2341 accaaacacc atgtttaaaa acttcggtat ctgattccaa atcataaatt tccatatttt
     2401 ccgaagcatc taaaaatgct tctggtcctt tgtctgctcc ttttccccaa gttgatgttt
     2461 tatcgtaagg aacaggcaaa attacaactt ttgcattttc atagtctgaa tattcctctg
     2521 gaatacctgc ataagtacgt ttagacataa ttatttaaaa tttcaccaaa attaaaacaa
     2581 ttaataagta tatccaagag ttttaagcat tgatttatat gattgttctt tagaatacag
     2641 tctggtagta actttaccgt cttcgtcttt gcttataagc acatgttttg gtgcgggaat
     2701 aagacagtgc tgaattccac caaaaccacc tattgcttct tgatatgctc ctgtattaaa
     2761 aaatccaata tataaagttt cttcttcatc atattctggc agataaattg cgttcacatg
     2821 ctgctctgaa ttatagtaat catcgctatc gcaagtaagc ccaccaagaa aaaccctttc
     2881 gtattgtgat tcccatttat ttattggcaa cattataaat cttttactaa ttgaccatga
     2941 atcaggtagt gttgtaataa aagaagaatc aatcatattc catttttctc tatcgttttg
     3001 ttgcttttga aagactactg aataaattgc accaccactt tcaccaacag taaatgagcc
     3061 aaattctgta aaaatatctg gttcaggaac tttgtttgca tcacaagcca tttttatttg
     3121 attcacaatt tcttcagcca tatattcgta gtcgtaatca aatgtcaagc tatttttaat
     3181 aggaaaccca ccaccaatat ttaaagctgt taattcagga caaacttttt taagctcgca
     3241 ataaacattc atacatttgt tcaattcatt ccaatagtat gcagtatctt taattccagt
     3301 atttataaaa aagtgaagca tttttagttt tactcttgga ttattttcaa taaagtcgtg
     3361 ataatattgt acaatatttt tgtaacctat tccaagtcga gaagtataaa actcaaattt
     3421 tggttcttcc tcagaagcaa tacggatacc tacgctaaac tcaccttcaa cttgctcaag
     3481 taactgatct agttctgaat aattatccaa aactggaata accgaagtaa aaccatcgtt
     3541 tatcaatcta gctatatttt caatataagc tggcattttg aaaccattgc aaactataaa
     3601 tttattttta tcaatttgtc cagattcata aagtgctctt acaatgtcaa tatcaaagtt
     3661 agaacttgtt tcaatatgaa tatcattttt caaagcttct tctaaaacga aagagaaatg
     3721 agaactcttt gtacaatagc attggtaata cttgccatta taatttgctt tagttaaagc
     3781 tacattaaac cacactcttg ccttttttat attttcacta atttttggca aataagtaaa
     3841 cttcatagga gttccatatt gtttaactaa gtccattaaa ggaatatcat aaaaataaag
     3901 ttctccatct tccacttcaa attcctctgt tggaaattcg aatgactgtt caattaaatc
     3961 tatgtacttg gtcttcatct gttaaaataa tttaaagtaa attagttatc catattattg
     4021 ggttgcaaaa gtaagtcatt tacttcaata tctatagcag attaatggaa tttttcatca
     4081 ctagactaaa ttttatattt aaacagtact atttcataca aacatactac tttaacaaaa
     4141 caaaggcaat ttttcgttat tcaacaaaga attaaaacta tttaaaaacc tatctaaaaa
     4201 caaaagtaag taagttacat agaaattttt aaacgcaagt cggtaagtta gacattgggc
     4261 gatgccattt tacgatccgt ttctaagttt ttaaaataag gaataagttt atgtatttta
     4321 tatactgcaa gtgctaagca agttcctaaa attgctcctg ctactaaatc ggctggataa
     4381 tgcacaccaa gataaattcg agagtaagaa acaatgcttg cccaaattaa aataaaccaa
     4441 gtccattttt tatttcttaa aaaaagcaaa gtaattaatg ctaagccaaa accattacaa
     4501 gcatgtcccg aaacaaaact atacaagcca cctttataac catcaattgt atgtacctta
     4561 tctgaaattt ccaaattgtg tgtaggacgg tatcttttta cactatgttt tattagattt
     4621 gagctttggt cggttaatgc aaaacaaatt atagacacta ataatggaat ccaaaatttc
     4681 tttttaaatt ttctaactaa taaaaaaata aaaatagcca caacgatata ccaaatccaa
     4741 aaagaagata tgaatttcat aattggatct aaccaagcta agttaagtga gttaaaataa
     4801 agaaaaatat caatatctaa gttttctaag tattccatta ttcgttaagt ttgttccaaa
     4861 ttaaatcttt tagtttatca atatttttat ttgcaacagc agaaataaaa attgtttcaa
     4921 tattttttgg taattctgct ttcaacaatt ccattaattc atcgtctatt aaatctgatt
     4981 ttgtaattgc taatattctt gttttatctt ttagttctgg attgtattct cttaattcct
     5041 ttaataagat ttcatattct tttttaatat tttttgtatc gcaaggaatc ataaataaaa
     5101 gtattgaatt tctctctata tgtcgtaaaa acctgtgtcc caaacccttg ccttgtgctg
     5161 ctccttctat tattcctgga atatctgcca ttacaaaaga tttagaatct ctatattcca
     5221 ctattcctaa atttggaact agggttgtaa aaggataatc tgcaattttt ggttttgctg
     5281 ccgagactac agaaagcaaa gttgattttc ctgcatttgg aaatccaact aagccgacat
     5341 cagccaaaag ttttaattct aaaattattg ctttttcaat tccttcaatt cctggttgcg
     5401 aatatcttgg tgcttggttt gtcgaagttt taaattctgt atttcctaat ccacctttac
     5461 caccttcgag caatattatt tcttgattat cttctgtaag ttcgcaaagg aattctccag
     5521 tttcggcatc ttttgcaata gttcccattg gaacttcaat aatttggtcg gcaccatctg
     5581 cacctgttct gttagaagct cctccacttt ctccattttc agcaaaaata tgtctttgaa
     5641 atttcagatg taacaaagtc caacgatgag tgctagctct aagtattata tgtccacctc
     5701 tacctccatt tccgccgtct ggaccaccac gttcaacaaa tttttctcgt ctaaagtgag
     5761 tagcaccagc tccgccagat ccagagcgac aaaatatttt tacataatct acaaaatttg
     5821 atgtaaccat agattttagc tcataaattt atttactatt tcaaaaattt ctgcagaaac
     5881 tacagatatt ggtcggtctc cgtttatttt aaagcaatta gttcgcgaaa aataaaaatc
     5941 atttataata tgtgtattat ttttataaac ttcaagtctg tgtttaataa ttctaacatc
     6001 agaatcatct tttctgttgc tacttttact acgttcaatt agtctagaaa tcaaactatc
     6061 ttcacaaaca ttaaattcta aaaacacatt tacttttagg tttaattttt ccaatatttt
     6121 ttcaaattca acagcttggt tatagtttct tggaaatcca tcgaaaacaa attttgtatt
     6181 ttcatcacta gttgtgataa ttttttcaat catttcgcat acaatttcat caggcacaaa
     6241 attaccttta tctataagtg attgtgcaag tttacctaaa tcagtatttt tttcaatttc
     6301 ctttctaaaa atattaccag ttgaaatatg cacaaatccc aacttttctg caagaagttt
     6361 agaatgagta ctttttccgc taccgggtgg acctgccaaa attgctattt tcataagtct
     6421 ttttttacaa aaatattcaa ataaatttaa agtaacaaat attgctctat tatagtcaac
     6481 gaaaaaaaga caggttttgg tattgatgta ggttaaataa tatatacttg gtttatataa
     6541 aaatagaaag tcagaatttt ggaaatctga ctttcataaa caaacaaact aactatgaat
     6601 cttttactaa aatgattcta attaaaatta tgaaactaaa agggataggc aattatttta
     6661 tttatcgcct acccttttaa gaaacaaaca aactatgaaa aactatgaac taaaattctg
     6721 ctagtatttt cttcacgtct tctggagcaa ctctaccaaa agttttgtct cctatcatta
     6781 ctactggagc taatccacat gctcctacgc aacgcaagca tgataatgag aattttccat
     6841 ctggagttgt ttcaccaacg ttaattttta attctttttt cacttcttca agaacttttt
     6901 ctgcacctct aacatagcaa gctgtaccca tacaaataga gattggaaat tgtccttttg
     6961 gactcatagt aaaaaaacta taaaaagtaa ctactccgta aacttttgct actggtacat
     7021 ttaactcttg agctacagcc tcttgtactt cagctggcaa atatccaaaa tgtccttgag
     7081 ttttatgtaa aacattgatt agctcttcag gatcgttatt gaatgagtgg caaatttcac
     7141 gtattgcctt ataatcttgt tcttttaatt ttatttgtat tcttgacatg tctttattat
     7201 ttttcgatgt aaattacttt tttcttatca aaatactcag tatgcaataa gtgatgtgct
     7261 ttttcactca ttggttgtcc taagaattct ttatacaagc tgattatttc aggattttcg
     7321 tgagatttac gaagtttcat ttcttcatct gcacgataaa gagcagctgc acgtgctttt
     7381 agtattgaag aatcaccatg atgtaatggt tgacctccac cacctataca gccacctgga
     7441 caagccataa tttcaattgc atggaaatca gcttcgccgt ttttcaccat ttctaataat
     7501 tttctagcat ttcctaatcc atgagcaatt cctaatttta gttctagtcc atcaatatca
     7561 actgatgcag tacgtactcc ttcaagtcca cgtaattgtt tgaaatcaac tttttctaat
     7621 gttttaccag tatgaacttc gtatgctgta cgacaagctg cttcgataac accacctgta
     7681 ttaccaaaaa ttactgcagc acctgttgat tgtcccatta atttatcata atcttcttct
     7741 tctagatctt taagatcacc tatactttgt ttaattaagt gggctaattc gcgagttgac
     7801 aatgcaaaat caacatctgg atttccatta actgcaaact ctggacgttg tgcttcgtat
     7861 tttttagcta agcaaggcat tatagaaact actattaaat cttcacgagc tacgcctagt
     7921 ttttctgcga tataagtttt agcaactgca ccaaacattt gttgtggaga ttttgctgta
     7981 gatgggatat cttttaattc tgggaattga tattctaaga aattaaccca tgctggacaa
     8041 caagaagtta acaatggtaa tttaacgtct ttatcaccgt ttaagaactt agtcaaacga
     8101 cctaaaagct ctgtaccttc ttccataatt gttaagtcag ctgcaaagtc ggtatcataa
     8161 actttatcaa agcctatttt gcgtaaagct gcaaccattt taccagtaac taatgttcct
     8221 ggctctaatc caaactcttc gcctaaagct gcacgaactg ctggagcggt ttgaacgata
     8281 acagtttttg taggatctgc taaagctcta ataactctat ttgtatagtc gcgttctgtt
     8341 aatgctcctg taggacaaac tgctacgcat tgtccacaga aagtacaagg cgattgaatt
     8401 aagtcttgtt caaaagctgg tgctactaca gcatcgaaac cacgatttac tccacttaac
     8461 acaccgcatg tttgaacttc attacacatt gtttcgcaac gacgacacat aatacattta
     8521 tccatatctc ttatgattga tggagaattg tcttctttat aagaagattg ctctccttga
     8581 taatgtattt cgcgtacgcc taaatcttgt gctaattttt gcaaatcgca agtaccattt
     8641 tttgcacatg ttaaacattc gaatggatgg tctgaaagga ttaattccaa aacagtttta
     8701 cgtgcgttaa gaactctcat tgtgtgagtt ttaacttcca ttccttctgt aacttttgta
     8761 acacaagctg gtgccaagtt tctacgtcct gcaacttcta caacacatat acggcaacca
     8821 cctggattgt gttcaatatt aaggtcgtga agattcatat gacataatgt tggaatctca
     8881 atacctactt ttttagccgc ttgtaagaca gttgttcctg ctgcgacttg tatgtctttt
     8941 ccgtttattt ttatattaat aagactcatt ttatagcctc ctgttattta atgaatattg
     9001 cgttgaattt acacttttcg taacaagcac cacacttgat acaagtttct tgatttatag
     9061 tatgtggctt tttaagttcg ccagaaattg caccaactgg acaaactcta gcacaagcag
     9121 tacagcctgt acaagcttct ggatcaataa cgtattgttt taaagcaaca cattgaccag
     9181 ctctacattt tttctcagtt acgtgttcta catactcgtc ccagaagtta tttaatgttg
     9241 ctaaaacagg gtttggagag gtttgaccta atccgcacaa tgctgtatct ttaataactc
     9301 ccgacaagtt tttaagtagt tctaaatctt ctagagtacc tttaccttcg gtaatttttg
     9361 ttaagatttc gtgtaagcgt ttgttaccta tacgacatgg agaacatttt ccgcaagatt
     9421 cttcaactgt aaattctaag aagaatttag ctattgaaat catacagtcg tcttcgtcca
     9481 taacaatcat acctccagaa cccatcatag aaccagctgc caataagtta tcaaaatcta
     9541 ttggtgtgtc taagtgtttt tctgataagc aacctcctga aggacctcca gtttgaactg
     9601 ctttaaattt cttgtcgttt ttaattccgc caccaatttc aaaaattact tctcttaatg
     9661 taattcccat tggcacttcg ataagaccaa cgttgttaat tttacctgct aaagcaaata
     9721 cttttgttcc tttagatttt tctgttccaa tacttgagaa ccattcagca ccttttagca
     9781 agattactgg tatagaagca aatgtttcaa cattattaac atttgttggt tttcctaaga
     9841 aacctgattc tgctgggaat ggtggtttta ctgttggttc gcccctttcg ccttccatag
     9901 aatggattaa agcagtttct tcaccacaaa caaaagctcc agctccatat cttaattcaa
     9961 tatcgaaatt aaaaccagaa cccaaaatat cttttcctaa caaaccatat tctttagctt
    10021 gtttaattgc tatttgtaaa cgtttaattg ctagtggata ttctgctcta atataaatta
    10081 aacctttatc ggcacctata caataaccgc aaattgccat tgcttctaat actgtgtgtg
    10141 ggtcaccttc taggattgat ctatccataa aagctcctgg gtcgccttcg tcggcattac
    10201 acactacata tttttggtct gctttatttt tagcagctat ttcccatttt aaacctgttg
    10261 ggaaaccagc tccaccgcga ccacgcattc ctgatttttt aatctcatcg ataacttctt
    10321 gaggagtcat ttctgtaata gctttaccta aagcttggta agcatcacga gcaagagatt
    10381 cgtcaatatt ttcaggatct aaaaatccgc agttacgtaa agcaattcta atttgctttt
    10441 tgtagaaatc catatgtttt gaatcactta cgtgttcttt attttctgga tttgtataaa
    10501 gtagtcttgt tactttacgt cctttaatga tgtgttcttc aataatttct ttagcatcgc
    10561 taggttttac ttgagtatag aaagtattgt ctggaataac ttttactata ggaccttgtt
    10621 cacaaaatcc gaagcaacct gttaaaataa cttttgcttc gctttctata ccatgagact
    10681 ctaactcagc gtttagactg tcaacaatct tagcgctatc cgcagatttg caacctgttc
    10741 cgccgcaaac caaaatgtgc attttatatt ttgccataat ctttaaatat tttaatcaat
    10801 tgactgatag tttgctggta gaataccttc aatcatttcg ccatttttaa tatacttttc
    10861 aataatctca tcggctttag ctatgtcaac atagccaaaa acaactggat cctcgcctgg
    10921 acgagtaact tctattgttg gctctgcata gcaataaccc atatctccaa cttgtaaaac
    10981 taaagcttct atattacgct tatccaattc ttcgataaga taagccataa tttcctttgc
    11041 accagaggca ataccacttg tcgccatccc aactctaatt tgaataatgg cttctggtga
    11101 atcacttctt tcgcgaagtg taatcttatt gtgcaaatca tctttcattt ttctaagatc
    11161 tgcaagattt ttgattttgt tcatattttt aaagtattat gtgataaata atataatttc
    11221 cgatacaaaa ataaaaataa actgcttatt gaacaaattg ttttggttat cattttgctt
    11281 taattaggta ataatcagta atttataagc attctaaata acttttttgc aaaaccaaac
    11341 acaaaacact actaatcaat attttacaaa atatcaaaaa aagattttaa aaataaaaaa
    11401 ataaaacaaa tgttatcaaa cataaaataa tataagtatt atattttctc aaacaagggc
    11461 aatcctgttt tgcaacatat ttttttgcga caaaaattat gttttgaatt atttattttt
    11521 aagttttgaa attttaatta ttctatagaa ttagctatta attctttaat atttgaaagg
    11581 attaaatggt tatttaattc aatattttca aaaacagatt ttacatcagc agttgaaagt
    11641 tcaaattcat tatcatctga aatatagcgg aaaataaaat ttatatcaga gtattgacaa
    11701 atgaacattg caatagttcc tggcaagtcg cctattggtt gacggtcaat atggtttctt
    11761 acaaattttg catacacttt tgtccctact ccaagctcgg aagtaagttc aaattttcca
    11821 cctgtaatct cggcgtgata cttcaaaaga gctaaaccca ttccaatttt tctagttttt
    11881 cttgaagaat aaaaactttc gtttatttct ttcaattttt cgggtgaaat tccacagcca
    11941 gtatcagtaa ttaccaaagt aatagaattg tctttttctg aatcttttac ttcgattaaa
    12001 atttcgcttg cttttgctct aatagaattg tttgcaatgt ctataatgtg taaatctata
    12061 gttttcactt ttatctaggt tttatatttt ttaaagctga tctaatattt tcgaaactag
    12121 gtgtttccat ttccaattcg gtataaacag ttccaatatc tggaatatag tgtgcgtctg
    12181 agcttgtaat gaaacgattt aggctaaacc atgaaaaatt ctgcaaaaaa ttatttttct
    12241 cagcgtattt gcttaattct aaagcatcaa attttaaatt ttctggaata aataccaatt
    12301 gtgaacttaa actaaatcgt ggtttatcaa catgagcagg gataaaaata ccatttaatt
    12361 cataaacttt tttctgtaaa tcttcaattc cacaatcaat tgcattaatt aataatgtat
    12421 caatttggtc aacaatattt tcatcttcat caactacaag ttgataaccg aatttgtcgg
    12481 ggtcgttttg aaaaaatatg tattttgaat ctaaaaaatt ttgaaattcc aataatgtat
    12541 tttcatctgg cataaaacaa aggcagtgga cttcttcttt tgttgtaact tcggcaccgc
    12601 acaaaacaaa aacatcgttt ttctgtccaa tttttgaagc aactaaagca ttacgagttg
    12661 aattatgatc gctaatacct attatattaa gtccttttga ttttgcttcc gcaattattg
    12721 ccgaaggact catttccaaa tcgccacaag gcgacaaaac agaatggata tgtaaatctg
    12781 ctttaaatat cactttatta attcgtacaa ttttccgcaa atattaaagc aagagtctgg
    12841 agttgacaac acacaaactt cttcttcttt tgcatgttct aacatatctg ggtcgggttg
    12901 tccattacct ataattataa cagctgctaa atcttttagt gagcaaattg cagaaacatt
    12961 tttatgtgtt tgcaaagtaa tccaaatttg acctgcctta gatttaccca taacatcact
    13021 caaaagatca gaagcgtaag catcttttac atcaacattc tcgttagcta agtttaaaac
    13081 ttttagctcc aatttttcaa taatatcttt taaaagcata atttatttat ttttattttt
    13141 ataattccaa attctaccta atgtttcttt agatttgtcg cattcagctg acataaaaat
    13201 acagtcttct atttctgcat ttccgcgagt aatatcttct gccaatgccg agcaagttgg
    13261 tgcgccacaa gctccacaat caattcctgg taatattgct ttagttttat ttatactttc
    13321 caccatcttt aaagctttct gcaaattatc atctagcttt aaaattgaac gaggttttat
    13381 ttcgtcaata gtcatcaatt cttttactct attattaaaa tcttgactaa atactggcaa
    13441 gaaacaactt ctaccatcaa gctgattatc aagagattct gcacgtcttt tttgtttatt
    13501 tactgctaca aatctattat ttgggttaag cactcctcct gcacaagatt ggttacaaac
    13561 tctcatttct attattccat cactttcaat ttcatcattt tctattcttt ccaaaaagcc
    13621 tataacattt ctaattccat caacagcaaa actatttcct gcaaaatttt gtgcttctcc
    13681 gcccgatgta ctccatagaa tatctcttcc cgaaaggcta tgatttattg gttcggcagg
    13741 tgtattttct ttcgcaagca tttgctgaac tttattatat aaaaagtcta tatttattac
    13801 accgttaatt gatgaagaga cattttcgac tggactttta acagctgcaa tttttgcagc
    13861 acaaggcgtg atataaaata ttcctatttc atctttacta attccagcgt ctgttaatct
    13921 ttctaaaatt gtaatagatg ctacatctag aggagctttt agatgaataa tattttgtac
    13981 caaagacggg aacctaacct gaattagcct aacaactgcc ggacaatatc ctgatataaa
    14041 aggttttcta tcctgatttt tacgcatata attattcatc aattcaatca atactcctac
    14101 tccatgttcg acttcataaa tatgcgtaaa accaagtttt tttagacatg cataaacttg
    14161 cgaagttcta acattttcag caaattgact aataaaaact gctggtacta gagcaattct
    14221 gtacttatat ttacctagtt cagcaaagtc gtcttgtttt atataaattg ctcgcacagg
    14281 acatactcta aaacattcgc cacagtcaac acatttattg gcataaagca cagctttacc
    14341 tttttttatt cgtatcgctt ctgttgggca tgcttgtaaa caatgcgtac aacctacaca
    14401 aatgtcatgg tcaactttta aagcatgata aaaatatgtt ccttttggtt cctccatata
    14461 ttaagctaaa taaaatgtca tttccaaaac cgtaccttta tctacaaccg actggatatc
    14521 aaactcatca acattacttt taatatttgg caaccccatt cctgctccaa aacccatttc
    14581 tctaactttt gatgaagctg tagaatatcc ttcttgcata gctttactaa tatctgctat
    14641 tccaggacct tcgtcttcga tacgggcaaa aattttatcg gcagaaattt taactccaat
    14701 ttgtcctcta taggcatgtg caaccacgtt tatctctgct tcatatagtg ctataacaat
    14761 tcttttaatt tgtgaagcat caactcctaa ctgttttaaa gttttcttaa cttcactact
    14821 tgcacttcct gcacctgaaa aattaccgcc ttctatttta aaatttaatt ccataatttt
    14881 aatacactgg tgaaattccg ttttgatata aaagtccaca aactttgtaa acactaaagt
    14941 cggtttcgat aatacaaata tcattttctt ctgctaattc aagcatattc tcatctactt
    15001 ttttacctct agcaaaaaca atcaatttta tttctgccat ctcaacagtg cgaatagttt
    15061 ggacattaca taaacctgtt atcaacaaag tattttcaca tttcaatcta agcacatcac
    15121 tcattaaatc tgaggcaaaa gcatgcttga tttcacctat gttttctaaa cataccttgt
    15181 tgtttacaac tcttccctcg acaagctctt taatatctgc tactttcatc tataatagta
    15241 ttattttgtt tacaaagata agtacaaaat tattaaaaac ataattttaa tgaattatta
    15301 ttttattact ataacactat atatatataa tactgaaact ttcgcaaaaa aataaattta
    15361 tacaagattt taaataaaaa tttattgtat ctttgttgaa taagaaaaat aataaaacaa
    15421 atatggacaa caatctatta aacgaataca aatagtatta cgccatacga gctgagagat
    15481 atgctaataa cgaaaattac aaatatagtt atgaagcaga aaaaaaactt tctgaagcaa
    15541 tgcaatcttc ccagtcttta gaagatttta aaaataaaat gggaaattta aatgaattat
    15601 gtgctaacgc tttagttaaa gatgagacac taatggaaaa agcattttac gaaaaacata
    15661 aagaaaatgt tagaatacta gatgctgaaa gaattttgca aaaagttgac tcttgtagta
    15721 atgctacaga tttgggtatt atgattaccg aagaaacaaa taaaaacagc atggaaatta
    15781 catcagacga agctcatcgt gtgttagtag atgattggtt tttattagac aaattggaaa
    15841 tttacgaaaa cgctgaagta ccatcagaat ataagtcgga aatgaagcaa attgcaagcg
    15901 atataagaaa tagtataatt gaaaatgcta gaagtgtaga agaagacatg caggcatggg
    15961 aaaacagatg gcgtctaaag cctgaaatac tcttagaata tcgccataaa agactatttc
    16021 cttacgaaga caagcatatt gaagaacaaa ttgcaagata taaatcaatt attaaccgtt
    16081 aaactccata cattatggca atagatagaa tgatgttaga tccaactttg gacacttaca
    16141 gaaaaatgct taaagatttg caagagcaaa atattacagg cgaagatatg gataaaatgg
    16201 ctgaaattat agccagaatg gaacaattag gaaatgaatt gtctgatatt aacgattttt
    16261 tgggaaagta atgcaagaag atttatttgg aaaattttcg gcacactaca ccaaagcatt
    16321 aacaagtcaa tatcaagcac aaaattccga gaatggggga acttataatg atgctgcttt
    16381 attaaagcaa tgtgttgatg ctctcaaata tgcagtcaca acaattaaag acagctacaa
    16441 caaaactata gaagatgcaa aaaactttga tgcaaaagaa cataaagata agagtattga
    16501 atatttcgaa gaaacaacag gtacaacagt tggcaaattc tttaaaaaac aagcaaaaaa
    16561 agatttagac aaaactttaa aagaaaaacc taatgctttt gacaatagta tagaagttgc
    16621 tgtgttacac gatccagaat taattatcaa aggaattcaa gatttaattg atttaggcga
    16681 acaagaagga atgaccactc caaaattttt gcgtttgcaa attgaaactg gtctagacaa
    16741 agcaatgcaa gggacttcaa cttttagaaa agcattagaa ttccaacttg attctacatt
    16801 agcaaatcct acaccttgga ctttaaagct agcagaagaa aaacttagag tgtttgatga
    16861 attagctgca aaaaacaaat ttaacattcc aaatcttaaa gaacttgaac tcgctcataa
    16921 tgatatagat tacatatatg aaagagacat aaaaatttgg gatgaaatta tagaacgttg
    16981 gaaagatttg ttaggtgatt tagatgtatg gagtttatca cattgtagtt ttgcaccaag
    17041 tattgagcct tggagaatgg caagagaccc aaaacaagct acaattaaaa cacaaaaaac
    17101 aacaccagga atttttaaac aaaaagaaaa attgttgaaa aaatattttg gtctaaactt
    17161 tatggacgtg ttcacccacc atagttttga atggaacgta aaatataatt atatagaata
    17221 ctcgcaagaa tttacagaat ttttaatcga aaaagtctat ccacaatgtg tgcctttaaa
    17281 tagtttaaat tctgatataa ttaatgaaag agctagcttt tatccaacag gcagtaatcc
    17341 agatagagaa accaatccac actgcaatat gtatgcaaaa agacttagag atttttacga
    17401 ctctaaattt ggcaaaggaa gatatgattc taaatttgga gtaattaacg aaatcaacag
    17461 cgctgctaaa ccatgggatt gggatagttt taagtttaaa aataaaatat aattatattg
    17521 ctacttattc gtatcagtag gattgttgct taaataaaac aatcctttta aattgggact
    17581 cgtataaact tcataaatat aatgtttccc tctaattttt atttttttcc atttatttca
    17641 tattttgcca catcaactag cattggaact gtgtttttag aaatatcaaa catcatagac
    17701 atcaaggact ttatgaactt tacaaacttt acgaacttta tgaactttac gaactttatg
    17761 aactttaaaa actttacgaa ctttacaaac tcttaacaga tataaaaaaa cacatcttcg
    17821 ccggggagac gaagatgtgt taaaaaaatt aagcattgtt atttattaag gagaggattt
    17881 ttattcacat ctatttatat aaaccaataa gtttttaatt tgttcacaaa taaaacgaaa
    17941 aattttaaaa attattgtat ttattttaaa aattaagcct ttatttaata aaaaaacatt
    18001 atttttacaa acttgtttaa taaaagtaaa aaaatcccgc cggggagacg ggatttttct
    18061 tttaaaaatt aagcattgtt atttaaagga gaggattttc ttattataat gtaaaaccgt
    18121 tgtttttcat tttctctctg atgcaaagaa acaacaatat tttaaaaact agtgtttatg
    18181 caaagttaaa taaagttaaa ataatgttaa tgagcaaata atgatacaat tggtttatat
    18241 atatttgtaa tatgaagaag cgaaaaatgg tcaacggaat aattgtttta atgtcaattt
    18301 cattgatatt gataatcatt atgcaagcta cacacctctc taagtcttac aaaagctcaa
    18361 aagaaattgt tgaaagagga attgcagaag ctatttcgcg tagcatagtc gtattgcaaa
    18421 aacaagatgc ggtatttttt gtttatgata aacttcatca aaaaaataaa gttgttgaca
    18481 gtttagttcc cattgaccct tatttagttc aattggggtt aagacctatt tttaatcagt
    18541 attcaaatat acattttcaa tctttcccag gaacaggact aagctctgtg tcttttcact
    18601 cagaattttc tgatttatct tatatagagt cgtatatgtt tcaacaattt actcaaaaaa
    18661 ccatagagtt cgaccaaata attaatcaat tggaaactga gtttttacaa agacaaatac
    18721 ctattgaaca aagatttgac gcagaaactg ttgaaataac tcttaaaaaa tcattggcat
    18781 ctgttgggct taaccttgat ttcgagtttg ctattagcga caacgaccaa aacattaaat
    18841 taaagtcttc aaattttaat gctaaaaact taaaaaattg ctataaattt aatttaactc
    18901 ctgcatctgt ttttacaaat ccagatgtcc tactaattga ttttccaact aaagataaat
    18961 atgtattaga aactatttac gcacaattag gcacttcaat agtattaatt ttaatttttg
    19021 ttttaacttt tagcacttct ttatatgcac taattaagca aaagaaaaat gctgaaatta
    19081 aaaacgattt tattaataat atgacacacg agttcaaaac tcctattgcc actataaaac
    19141 ttgcagcagc atctatacaa agcaataatg caaaagaaaa tcctgaattt gtaaataata
    19201 tgctcaatat tataaatcaa gaaaccaaca gaatgaactc acatgttgaa caagttttac
    19261 aaatggcggt tttagataaa aaaaatgttc aattaaaaat atctactgaa aatataaatg
    19321 aattagttgc agaagttgct aaaaatattg agcttgtaat tttggaaaaa aacggagaat
    19381 taaaattaaa tttatgcgaa gaaaatccaa acttacgaat agacagagac cttattgcca
    19441 atgctttaga caatttgtta gacaacgcta ttaaatattc taaaacttct ccagaaatta
    19501 cagtaaacac ttatattaag aatgataagt tcaatatttc tgtagaagac aaaggaattg
    19561 gaatgtcgaa agatgtgctt aacaaaattt ttgaaagatt ttatagagct cacacaggga
    19621 atttgcataa tattaaaggt tttggtttag gtttgaatta tgcaaacgaa attgtaaacg
    19681 cccataacgg acatataaat gttaaaagta ctttaggaaa aggtagtact tttattataa
    19741 ctttgccaat gattaattaa taaatataat atatgaataa acaaaataac aataaacaaa
    19801 aacttttatt agttgaagat gatgtaaatt ttggcttcgt gcttaaatcg tatttagagt
    19861 taaacgaatt tgatgttgat ttagttagcg acggtaaaga cgctttccca acttttaata
    19921 aaaacactta taatttatgc attctagacg taatgttgcc aaacgttgat ggttttacta
    19981 ttgctaaaga tattagaaaa atcaacaaaa atattcctat aatttttctt acagcaaaat
    20041 cacaaaaaga cgatgtgtta gaagggttta aatctggtgc tgacgattat ctaacaaagc
    20101 cttttgattc tgaagtgtta ttagctaaaa ttagagctat tatacgcaga aacagcaaca
    20161 gaccaggaca aagtctagat aagaaaatta atataggaag atatgtcttc aacactcaac
    20221 taagaacgct tcaagacgga gatacagttc ataaactaac tccccgcgaa tcagacttgt
    20281 tacaagaatt atacttatat agaaataatg ttttagacag aaatcaagct ctaaaaaaga
    20341 tttggggcga tgacaattat tttaacgcta gaagcatgga tgtttacatt actaaactta
    20401 gaaaatattt taaggatagt gatactgtac aaataaataa tatacacggc tctggatttg
    20461 tactaatatt gaaagacgaa caataataag tcatataatc aattgaaaaa aaactaggtc
    20521 gcttattagc attatttatt gttgtaagat gaaatttgtg aaaatattaa aatatatttt
    20581 aatacttccg tttcttatat tagtaaaact ttatcaatgg ctaatatctc ctttaattcc
    20641 accttcgtgc agattcactc ctacatgctc caactactcg ctagaagctt ttaaaaaaca
    20701 tggtccaatt aaaggtttta ttttaagtac ttggcgaatt ttaagatgca acccttgggg
    20761 cggacatgga cacgaccctg tacctgaaaa attttatatt tttaaaagaa aaaaatgatt
    20821 taaaactatg gattggataa tcttatttat tgcaggttta tttgaaactg gatttgcgtt
    20881 ttgccttgga aaagcaaaag aaaccaacgg cacagaaatg tatttgtggt attttgcatt
    20941 tttagtttcg ctaacaataa gcatgttgtt gttaataaaa gctacaaaaa ctttacctat
    21001 tggttctgct tacgcagttt ggacaggcat tggtgcagtt ggaactgcat taatagggat
    21061 atttgttttt aaagaaccta caaatttttg gcgtttattc tttatcttta cacttattgc
    21121 ttcaattatt ggattaaaaa ttgcatcaaa ttaatctgaa ctcaaagaat ctcgcattga
    21181 tttaaaacct tcgccaataa tttcgtgtgc ttctgtaact gttataaaag cctttggatc
    21241 aatttcatag atatagtctt ttaataattc aaactctcta cgacttacag tagtgtaaat
    21301 tatttgtttt tcttcttggc tatacattcc tttcccatga aaaattgtac ctccacgatt
    21361 caaatcttga ataatagttt ctttaatttc ttcatattta tctgaaataa taaatatagc
    21421 tttttcgtaa ctagcacctt ctaaaataat gccaacagcc ttacctgtaa tataaatcac
    21481 aagccaagag tataaaggaa ttgtccaatc tttaaacgct actaaactta taagaacaat
    21541 agcggaatcc acataaatta ctaaagttcc aagcggaatg cgtgtatatt tattaagaat
    21601 catggcaata acatcactac cgccagaaga agcacgagat ttaaaaacca aaccaagccc
    21661 cactcctatc aaaactccac caaatattgc cgacaataat gtatcatctt taactaaagg
    21721 gacatcacca ttaaagtaag ttaacaaatc gacaaaacca gccattgaaa caaaacccac
    21781 aacagttttc caaccaaatt ttggtcctaa taattttaag cctaacaaag taattggaat
    21841 ttcaaaagcc aaagctgtca aacctattgg caaatcgaat aaatggtgaa taactattgc
    21901 aataccataa acgcctccag gtgttatttt atatggggaa ataaaataca cataacctaa
    21961 agacataata aatgttccac tcaatatcaa agccaaggac ttaaaccact tagccgaaaa
    22021 caatttttct ttatttaaga atttcataat cttctaaatt tggaatgcaa aaatacattt
    22081 tataatgaat tggaaaaaat aatcagttaa aagtcgaaag ttaaaagaag ttttatgatt
    22141 ggcatttctt tatgaacttt acgaacttta taaactttta actgatagtt caattgtttg
    22201 ctccctatca ggacctagtg ataagtattt aatttttact tgtgtttttt cttctagata
    22261 tttaatatag tctagcagtt cttttggtag ttcttcgagt tttgtgcatt tggtaatatc
    22321 ttctttccag cctggtagtt cttcataaat tggttcaata tcatcgcaca tatcataagg
    22381 catataatca attatttcgc ctttatactt atattttgtt gcgactttta ttgtatctaa
    22441 accgcttaac acatcagatt ttgtaacaat taaatctgta actccattta tcattataga
    22501 atattttaaa gctaccatat ccaaccaacc acaacgtctt ggtcttccgg tagtagctcc
    22561 atattcgtgt cctttgtctc tcatatattg tccaacctca tcaaacaatt ctgttgggaa
    22621 aggacctgaa cctactcgtg tacagtatgc ttttacaatt ccgaaaactt ttccaatttt
    22681 ttgaggcgaa actcccaacc ctgtacaaac tccagacgaa actgtgtttg aagaagttac
    22741 aaaaggataa gaaccaaaat caacatctaa aagagttccc tgtgctcctt ctgccaaaac
    22801 agatttattt tcgtttattg atttattaat aaaaatttca gagtctataa atttgaactt
    22861 tttaagaaat tcaacagcat caaaccagtc tttttctaat ttggaataat cttcattaaa
    22921 attatatcct ttaaaaatct tttgatgatg gtcttttgca gtattatatt tttctttaaa
    22981 actttcggta aatatatctc ctatttttaa agctagtcga gcagttttat ctgtatatgc
    23041 tggaccaatt ccctttaaag tagagcctat tttatttttg cctttatact cttcataagc
    23101 tttatcaagc aatttgtgag tgggtaaaat taattgtgct ttacgagaga tataaaaaat
    23161 ttcacttaaa tctacaccta atttttctgt atcttcaatt tcttttttca aaataatagg
    23221 gtcaattaca actccattac ctattatatt aatggcattt tttctaaaaa caccacttgg
    23281 gattgtgtgt aaaacatgtt ttattccatt aaattcaaga gtatgtccag catttggacc
    23341 gccttgaaat ctagcaatta tatcatattt aggcgtaagt acatcaacta ttttgccttt
    23401 accttcatcg ccccattgca aacctaataa aacgtctatt ttcatctttc aatattattt
    23461 tttatattct tctttctttt gacattcatt gcaaatccca tataaataca aagagtgatt
    23521 taaaacttta aaatcgctgg ctttagaaat agttttttca atttcagcaa ttcttgggtc
    23581 gcaaaattct ataacttttc cacaatctgt acaaatcatg tggtcgtgtt gcatacaagc
    23641 ataagctttt tcaaatgtgc ttatattttt tccaaattga tgtttaacaa tcaaatttgc
    23701 gtctaacaaa atatcaatat tgttataaag cgtagctcta ctaacacgat aatttttatt
    23761 tttcatagat atatgcaaac tctcaatgtc gaaatgacca tttctagagt aaatttcatc
    23821 taaaatagca aatctttctg gagtttttct atatcctttt ctttccaaat aatcagtcaa
    23881 tatctgacgt acaggaatat tatattctac ggctttcatt gtttgttatt ttttagacta
    23941 aattaaaata atttttctca tactcaaaac ttattttaaa caaattatca acaaaatttg
    24001 aaagtaaatt tttaattaaa tcgattttaa tatctttgaa aaaatattta ttgtgatttt
    24061 tttcagatta atattagata gtataaaatt ttcgttttcg tcattaatta ataataagtt
    24121 aagaacgttt ttatcgcttt taggaattac cattgggatt tttagcataa tctctgtaat
    24181 ggcgactata gattctttag aaagaggcat taaaaacaat ataggacagt taggttcaaa
    24241 cactgtttat atccaaaaat ggccctggat ttttgaagca gactttcctt ggtggaaata
    24301 tattaatcgt ccttcgccaa aattatctga acttacacaa attagagaat tatcaacttg
    24361 tgccgacaag attgcttttg cagcttcgac attgcaaacc atagaatacg aaaacaaaag
    24421 tttagacgat gctggtgttg ttcttagcac acatgattat atgcaaataa actttatgaa
    24481 tattgaaaaa ggtagatatt tttcacaaat ggaatcacat actggtcaaa atgtggctat
    24541 cttaggggcg caatgtgctg aaagtttatt tgaaaatacc gaccccattg gaaaacaaat
    24601 taagctaaat ggcaaaaaac taactgtaat aggagttttc gaaaagcaag gagaaaacat
    24661 gtttagttca ggctctgatg agttggtgtt aattcctata aattttggaa aaacaatttt
    24721 tgatattgaa aacgacgact taaatccatt tataatagtg tcggctaaag aaggaattac
    24781 ctccggtgaa ttgattgatg aactcacaat aattatgagg acaattcgaa aattaaaacc
    24841 tttagaagaa gataattttt cgctaaatca agcaagtatg attacaaaaa gtttagacaa
    24901 aatttttaag atactcgatt tagcaggaat aattattggt ggttttgcta ttttagttgg
    24961 aggttttgga attgcaaata ttatgtttgt atctgtaaaa gaaagaacca aagaaattgg
    25021 aatactaaaa gctattggag caaaatcata tcttatttta attcaattct taacagaagc
    25081 gacagctttg agcttagttg gcggaatttt aggattaata ttagtatggt tgttaaccgc
    25141 tgttgcgcga agtctaacgc cactagactt agctttaagt tttggaaata ttatgtcagg
    25201 aacgctaatt tctatttgtg ttggaatagt ttcaggcttt gctcctgctc gcaaagctgc
    25261 taaacttgac cctattaagg cgataaatca aggaacttaa gttaaaagtg tcaagtttca
    25321 caaggttgtt tatgataact tgtattaatt tgtggtaaag ata
//
LOCUS       DAOPNX010000029        25040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_2518_length_25040_cov_6.261357, whole genome shotgun sequence.
ACCESSION   DAOPNX010000029 DAOPNX010000000
VERSION     DAOPNX010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25040
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2518_length_25040_cov_6.261357"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PKN53_04745"
     CDS             complement(<1..725)
                     /locus_tag="PKN53_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOD88296.1"
                     /translation="MKLKIFTYIGILLLISSFKLSAQQIGNHRNYVFNIANQNPAAIA
                     MKEIPEILLNSRIQWTGFSGAPRINSIYGKYLFRSDMGASAALVHDRIGISQRLAFNL
                     GYSYIIRTKNFNISFGLDMNFSQLKLLSSQIKVYETNDQILNFETDEKIWKPDANAGI
                     LLNANNYFVGFSILQLFKSNYFFSGSNADISGLIKNARHFFITGAYNFKLEDSKHSFT
                     PILNMYFAKSSPFKFDLIANYNY"
     gene            complement(728..4639)
                     /locus_tag="PKN53_04750"
     CDS             complement(728..4639)
                     /locus_tag="PKN53_04750"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOD88297.1"
                     /translation="MKHFVKISYLFLFILLSLNIFGQENTLQHEMLYQSHNQNMWGPD
                     YAYGIDVDHTFFDIDINESWGFSQISEFLGQQFGVGMNVGIIALLRSTFEAHGFYTGS
                     FSLDYPIKTTLEFPEDFSFNYGGPATIHTSYEVTDGWALETSFPPVGVITLDFEYQFD
                     PFMNIVVCVFSCDTIHLIPASVSLPYTLDTLFHINAETQYCVYPCYVGDQFTFCHDYE
                     LPIDIDFTDLVGFNFEAHVDLPEVFTEDYIEPGSNCLIAKGEDPYMLVELDVLGFLYV
                     MAGYIPPPEGPQIQQAIAFLNDTLQYPINTPLGDIIFQIEYSLLSVYLNIENTLHQDI
                     YFCPTIWLTLDFPVEMPYIVTDPQNSNAEVESGFGDTITMAVGNDLTITYPCHDWDSM
                     YVGVKYNIQPTIRNHTWDSIAFSFVIEALSASITIVTPFKSQIADNVMPSFELPINIG
                     KPIVSEPIAMQGYYEDEEFIQGLEKDIGPWDIGPLFEWTVSLGYVPVTWFDQTWELMH
                     FAEDIVFPGTYIRPYDKSEVNSLLYTEQAFCYGQTDGYIYAQAQNGIAPYEFQWSTGQ
                     LTQNSFADIDSIYGNPGYYMITITDAQNCESYDSISVAINPPIIHSLTASDLDCYGFF
                     TGSITTEVSGGTFPYFFEWSNGSHQQSPTDLTSGWYYVTISDFRECFVTDSIFISQPT
                     SPITIDYTKTDLPCHGTDFGSIDITLSGATPPYTVLWSSGQTYQDLYNLNAGTYTITA
                     TDANNCSWSQSITIIEPDTLIANINSSNIPCFGLDNGSLVVQASGGTQPYTYHWFHDP
                     TQSSHIITNMHPGYYFVSVVDANGCSDTASTHITQPDELILVEQIHNVSCKGGHDGYI
                     TVDVSGGTPYYNITWSNGYFSDSIGNLPIGSYTITVTDQNACKNIQTYTITEPANYLT
                     SNFSEINNVSCFGYYDGSANVIPTGGTPPYTVYWEEGVEQQDFTGLGMPANTYYNITV
                     VDANSCHYFDSIRFTQPSMLQLTGKTDPVECGLKAGSGTVMPEGGTPPYSYLWISNET
                     TQTATNLPYGQNQVIVTDANNCSDSLTVFVNKLGKIYGSYNVIEPNLCFNDSIAVAIA
                     KLEDGQQPIHFIWSNGHIGDTAKGLPAGEHFVFASDKYNCTDTIRVLISEPDSLNPNF
                     KLTPPTCSGIYDGAIEVNPSGGTPEYTFLWDNNSEENNISELREGNYYISITDANNCL
                     YIYNVDLMELTYCITIYNTITPNGDGANDTWIVENIDKFPRNKIWIYNRIGNLVFDTE
                     NYQNDWGGTFKGKDLPEGTYYYIIDLGDGKSLFKGHITIIR"
     gene            complement(4641..5159)
                     /locus_tag="PKN53_04755"
     CDS             complement(4641..5159)
                     /locus_tag="PKN53_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88298.1"
                     /translation="MNKLLIIIVLIFSSLSVFSQTDELEVFVQEQNAKLEYNPNKLVF
                     APNTNVKMIPPEYFEVDESINGFVHKGSAATIQVMEINGVSYRDISSSMTKEYIESQN
                     YIFKEKVNLQTATGKDAVIFFVEFTSGEMVYERAMFFTGEENTIWVNFNYPLNMKKLL
                     FPAIEACLISIE"
     gene            complement(5245..9387)
                     /locus_tag="PKN53_04760"
     CDS             complement(5245..9387)
                     /locus_tag="PKN53_04760"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOD88299.1"
                     /translation="MRKIFTFLFSFLSAVAFGQVEFETTETFNSKDASINTYKITSPI
                     IQDRNGGYGFYLKTPVEFTSFALGWVSSTEIYSAGSFEIVYKVHKPNKGWTDWEIDDG
                     FTKPNETKSNYYKSNLMFGLDEWEHDSIEFYIHPPEGEIISEIYLILLNVRPTIITNT
                     ETKSTKSGAKACPEFPSPIITRSGWCNGVSNCLYPTYTVVYRNPTHTVIHHGASPSSY
                     TDGAAVVRSYWNYHVNTLGWSDIGYNYLFDKYGNFFQGRHNPNLPNQDVHGAHAGYSN
                     TYSIGLNFLGDSDSPGTAPTQPQLDKCAEFLAWWYDYRGFDPTSMASIIKQDNSGYIN
                     VHRICGHRDVNPGGTTCPGDALYALLPSIRTATQQILDICDGSFDTQAPTTDIQTQRK
                     WYNSDFEVNFNDQDNEGGSGVNYSFYQVMDFDGTEWRANGEHGFFNDNFETEINAEWT
                     EYTGEWSISENHLKQTNQTSTNTNLYAYVNQVQGETYLYHWKQKVSGSGNNRRAGMHF
                     FCSDPEASGRQTSYMLYLRVDPTLGTGSGTAEIYRYNNNSYDTSEGGFFHFESYDLQP
                     NIWYDVKIILNTTTGIISLYIDNEFVTSITDPTPFESGNSISLRTGQSICEYDDIKMY
                     KTRSASEIITTQLSPNAEIRYQSPNNTQEAARIRTQIIDNANNWSESKSKNIYTDFDN
                     PETDIEVTGTWHTDNFSAYFSDNDQLSGVEKGFFNISDFDGTKWTANKNKGHFFDDFN
                     NALSTDWTNQVGNWTTNSEGILIHNDESVGNSNIYAYLNQELSNKYLYEFDLKVEGSN
                     VNKRAGFHFFCDNPELSNRGNGYFIWFRLQDTQSLEFYRVTDNVLSLDKTFTINFAAE
                     TWYNIKILYDRVTGEFFVYQDNKLIGEHKDTNPYFTGSYISFRSGNSIMSIDNFRTFR
                     SRYSNQNTEIVCNATDGDLRYSNPNPDIAAAKISSMCMDSAKNLSAIITKNYNIDFSK
                     PTSISNVNDGIAQDVDSSNDSQNLSGNWTESQDPNSGISAYFYAIGTSQGGTDYLTWT
                     NNGLATNFTITNANLEIGQTYYISVKAKNGAGLYSDIATSDGVLIQSIICPDNQYLSI
                     NDATLELNLATPQSGQYYYNNNPITSFNPNEYGVGNYLITYIYTDENNFVDSCTFTIY
                     VYNPNVVECTENFEICSNANEFALEGATPLGGTYSGDAVVDGVFYPENASLGINIITY
                     SYESEQCSFEIVVKQAPEVFCPNDINLEENDSPLPLSGAEPEGGNYYLNGNQITYINP
                     ANYEIGDYTIVYTYIDETTQCSASCYFNLTISPFTGLHTDTNFDFDIYPNPNTGKFKI
                     LLPNQNEEYNLQILNIHSQIIEEKQINSNEDEMNFNLSSGIYFVKLSNSKVNLVKKIF
                     VE"
     gene            9497..10945
                     /locus_tag="PKN53_04765"
     CDS             9497..10945
                     /locus_tag="PKN53_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD88300.1"
                     /translation="MPKFFKLLKLYPKTYWISNVMELFERWAWYGMFMLFALYLTGST
                     DAGALGFSQTQKGMMMGTVVGLLYLMPIFTGAIADRFGYKKVLIISYIILSSSYFALS
                     LVTSFYAVFIVFLILAVGASLFKPVISACIAKTTNDQTSSLGFGIFYMIVNIGAFIGP
                     IVASLARQHDWKYVFYISSAVILINLVLVILFFKEPEREIKQNKFSQELKIIFKNIFE
                     ALKDMKFVVFLLIIVGFWTMYNQLFYTLPVFIDQWMDTSIVYNKLHSVWPWLAETIGT
                     PEKTIAAEMLTNLDAMYIIIFQIAVSYFVMRLKPIHSIISGFLVATIGLFLTFSTNNP
                     MFLFVSILIFGLGEMAGSPKITEYIGKIAPPNKTALYMGYSFLPMAGGNYLAGILSGS
                     VYQKYSDKISLLKMEVSSRGLNIPEISENFSQTDYINAACQQMNMSQGQLTEYLWTNY
                     QPYNIGIIFAAIGIGTVIAMLLYNWYLNRRKA"
     gene            complement(10946..13771)
                     /gene="uvrA"
                     /locus_tag="PKN53_04770"
     CDS             complement(10946..13771)
                     /gene="uvrA"
                     /locus_tag="PKN53_04770"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOD88301.1"
                     /translation="MNKKENFISVEGARTHNLKNLSLDIPQHQLSVITGLSGSGKSSL
                     AFDTIYAEGQRRYMETFSAYARSFIGSMERPDVDSITGLSPVIAIEQKSTIKNPRSTV
                     GTITGIYDYLRLLYARASTAYSHVTGEKMIKYSDEQIINIILKDFKDKAVYILAPIVD
                     GRKGHYKETFENLRKKGFLNARIDGEIREITYNLRLDRYKTHFVELVVDKLIIKNQDR
                     NRLKKTIDLAMSLGKNIIMVLEKDSDKPRFFSRFLMCPTSGISYREPAPYTFSFNSPQ
                     GACPKCNGLGVIADIDINKIIPDPSLSIYNGGISILGKYKNSLPFWILEALAEKLNFS
                     LKTPIDELNEQAIDAVLFGYPESLQLKNTPLGLTSNYSISFDGIVNQIKAKQEDEEKD
                     PYSNKSSFRFNKFITCPKCNGTRLQAEALQFKIAEKNIAELAEMDMLNLSKFLDTVLD
                     KLDNKERLIATEVIKELSSRVSFLLDVGLDYLSLNRSANSLSGGESQRIRLATQIGSR
                     LVNVLYILDEPSIGLHQRDNKRLINSLKQLRDRSNSIIVVEHDKEMITEADFVADIGP
                     GAGRKGGHLVFAGKPSDMLKSDSLTSQYINGKLKIEVPKSRREISDKKIIIRGAKGHN
                     LKNIDLEIPLGVMICMTGVSGSGKSSILNRTLVPALSRHFYNSNAFPLDHDKIEGIEN
                     IDKFIEVDQSPIGRTPRSNPATYTGLFTDIRELFASLPEAKIRAYKAGRFSFNIKGGR
                     CETCKGAGVKTIEMNFLPDVYVQCETCRGKRYNRETLEVKFKGKSISDVLELTINAAV
                     EFFENIPHIYKKLIVLQQVGLGYINLGQSSTTLSGGEAQRVKLATELMKRETGNTLYI
                     LDEPTTGLHFEDIKVLLKVLNSLVDRGNSMIIIEHNLDIIKSADWIIDMGPEGGERGG
                     TVIAQGKPEDIIKNKNSYTAKYLKEEL"
     gene            complement(13764..14741)
                     /locus_tag="PKN53_04775"
     CDS             complement(13764..14741)
                     /locus_tag="PKN53_04775"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOD88302.1"
                     /translation="MDKKKLMKIPFFIILGLGLGLIFANSFIFSSSEKANQIKETEIQ
                     PTTIPYKVFQPYIPDTLSFCGENVPLQNFDVYEALDYELIVNTYRHSSTLIYIKRANR
                     YFPEIEKILKEKGIPDDMKYLCVAESGLANVVSPAGATGFWQFMKTTAGEYNMHVGKG
                     IDERYNHTKSAIAATEYLHKAYKVFKSWTLAAASYNMGMGGLQKDIDYQKVNSYWDLH
                     LNQETARYVYRILALKLIMSNPKLYGFDIDEKHLYKEIKTQEIVVDTTISDLVEFAFK
                     YGTNYKMLKYFNPWLTGKSLINPNKKEYKIVLPAKNARECMKQSKNNDE"
     gene            complement(14751..15809)
                     /locus_tag="PKN53_04780"
     CDS             complement(14751..15809)
                     /locus_tag="PKN53_04780"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOD88303.1"
                     /translation="MNYIIIITFIVSFLFSLYLNNFLIKSPLKFLVKKSNKSAIRFSS
                     QSKPIFGGLSFYFVFLLTMIVILFLKENIYFSKEYISLFLVVTLSFLMGLADDILNTS
                     PIFKFIIQIVCALILIYNGIYIIISPMDWMNYLITILWVVGIMNSINMLDNMDGVSSA
                     SSLSIFIAVAIYLIANGGENSILSLIILVSTIASLLSFLFYNWHPSKMYMGDNGSQFL
                     GILLAWAGIKYFWNAIPITEFSYGFNTMQFLIVFLAFLVLITDTTTVTINRLLRKKSP
                     FIGGKDHTTHHLYYLGLPTDIIALVFFVLNSIGLALAFCLIQKSSTIAYSQLWLFFLY
                     PITVFLLLYINTKVSKEK"
     gene            complement(15806..18256)
                     /locus_tag="PKN53_04785"
     CDS             complement(15806..18256)
                     /locus_tag="PKN53_04785"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOD88304.1"
                     /translation="MDENKTSILNQKFDTRTFLIIVKKNIWVVVLLILICLGTGFAFH
                     RYSTPIFKTSTIIQVKNENKTNQILGISSNLVDKDLAPVIELIRSNEFLKTVVAELPL
                     DISYFRKGTFLSTELYGSTPFEIKYNITNNVICDQNIDLEFKDYKCLISYTLGTEKYE
                     YTIQPEEWQTIYGMEIYVHFPSQKDLELELSPQNKSLYFFIINNPNTILKNLSKRLNV
                     QILNETAGTIKITYQDFNARKAAEITNTIAEKFIEFDEAKKKESATNIIKYIDQQMEN
                     VFTQLNANEKDLHNFREQNNITNISDEYIFNKTNIYSSQISDIESKLLNVDFEIMTLE
                     KMLDKLRSNPDINTYELLAMLSGQKSEAFLSAMINSLQELLNKKEILLFDLTSNSHKI
                     QTIDEQIKSKKETIIDFTNSTINRLKAEKTELIKKNKELENIAFKESKYDEIEYARLQ
                     RLYAINEDFYAQLIKSKAETLISQAGYVSNNIILEKANIPSAPDFPIFSTIMTIAFLI
                     SLVLSFIFLAIKYLLYNKILSIDDITNHTNIPVVGGIPLSKTNFDVSQMVVHLRPKSM
                     LSEAFRNIRTNLEFYPIKDKSRIITVSSTIAGEGKTFLALNIGAIYAMSGKKVIILDL
                     DLRKPKLNQCFNVENIKGVTSILLGKNSFQECINNSSLEGLDFITSGPLPPNPGEFIL
                     SDKYNMLIEQAKQNYDIIIIDTPPIGIVNDALTSYSLADNQLYVMRAGYSPRAFINNL
                     NYFSKSIQLKNLAIVLNGIEKSIASYGYGYKAGYGYTKKTAYYGISYLNNTHNSYYGE
                     EEITKPKFFNKFRKKNKK"
     gene            complement(18262..19047)
                     /locus_tag="PKN53_04790"
     CDS             complement(18262..19047)
                     /locus_tag="PKN53_04790"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOD88305.1"
                     /translation="MKIKILFISSLILTIFFGNSCRLLRPSDMFKTDKDYPKSEFEVS
                     KNEYIIQPFDKIGLRVVSNTGESFFGTGSMENANANANYQRNQHGSEFLVEFDGKIKL
                     PILGRIDIAGKTIREAELLLEKEYSNYFIEPFVLVQVINRKVMIFMDGGTKASVVEMP
                     NDNFTLIEAIAQVGGVTDISRSYRIKLIRGNLTQNPEVFYWNISNIKELKSSNILLEA
                     NDIIYIDSRPQYFTRVLREVSPYLTLITTLVTIYGVFFKLKQL"
     gene            19226..20323
                     /locus_tag="PKN53_04795"
     CDS             19226..20323
                     /locus_tag="PKN53_04795"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOD88306.1"
                     /translation="MKRLFIIFIICFGWNIVYSQSYNSYYHQFEMRANYGLTIPHHTC
                     LKYLIEKNIYIGEITYSIKTDGSEPWQHVWRFPELGVGYLMGSLGNINVFGFSQSLYA
                     FYGVPIVETGNFIFKYRFGCGLAYVSEKFDARANYFNIAFGSYLNAHLHFSLLVDYKF
                     PDIPLYLTAGFAYNHFSSGAIETPNLGLNQVTGNFGIKYMYSMYNYSLPKRTVPYQYD
                     KDFEFSAYYAVSVKENSTYENKKYIINSLICDFASRLNNQRSLGVGLNLIFDPSIKPL
                     LEEDYTGAHNLLRVGVHAMQEVYLTDDLSVIMHLGTYAFNKYKDGKKILVYSKLGVRY
                     TFAERYFANIVLKTHTTTADYIEFGLGFRIN"
     gene            complement(20320..21213)
                     /locus_tag="PKN53_04800"
     CDS             complement(20320..21213)
                     /locus_tag="PKN53_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOD88307.1"
                     /translation="MMSKSIVFLIAICLISFEVKAQDAKNFYIYFDINSYKIENKYKH
                     EFDKVDLSKIDKIEIYAYTDFLGSKELNDKLSQQRANATKTFLINLGFNSDKIITCQG
                     KSVHENSSFENRTNESDRGIKEHRKSEIIITFNVEEQTKSYTYQDTISFETKFDKKTE
                     HTDLANIKAEDLVKGNKFNIKNINFKGGTPFFLPTAEEPLMELLQLMRENPNLKIEII
                     GHICCQDESEPDGYDHTDGTYTLSLNRAKAVFIFLRKGGIEASRMIYKGVGSSQKLYP
                     REQNSWEQSQNRRVEIHVLEN"
     gene            21508..21855
                     /gene="rplS"
                     /locus_tag="PKN53_04805"
     CDS             21508..21855
                     /gene="rplS"
                     /locus_tag="PKN53_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOD88308.1"
                     /translation="MELMNIVNQKMAIEKEFPKFKAGDTITVSYKIREGNKERIQEFR
                     GVVLQINGEGRTATFTVRKMSGAFGVERIFPFASPFIDAITVNKYGRVRRARIFYLRG
                     LTGKKARIKERRF"
     gene            complement(21950..23971)
                     /locus_tag="PKN53_04810"
     CDS             complement(21950..23971)
                     /locus_tag="PKN53_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOD88309.1"
                     /translation="MKIKRHILLLLLCISYSFSFSQSLYDYCNEIDDKKIIKDFNKSI
                     EYINSEKFDQAELLLSKIVDEEPEFTEAWLGVAEINYIKYKNSKDAKSQNKFYANYAR
                     SLEKVASTCPHYNNWSVNYTLGKMFYEKEDYENAKKYLDLYTINAPKESKHIKEAELI
                     LDYIDTYLELISNPVPFKPVIVEGISTVNDDFLPLISPDGSLAFFTHAYMKKDIASVT
                     TEKYTEEFSVARALDESGTRFSKGEPMPYPFNTGKNQGASALTIDNSTLYVTICEFVS
                     RTYDNCDIYYSVRKANGWSELINLGPNINGLNTWESQPSISADGKTLYFSSIREGNIG
                     FDPNNPTSDIWYSTKNEDGTWNKAQNMGPTINTPGNEKSPFIHSDSQTLYFSSDGHKG
                     LGGYDIFFSKYRNNEWSIPVNLGYPINTQAHDLGFVVNTQGTKAYFASNKLKGKGGWD
                     IYSFELYPEARPEKVFIVKGQLLDEVGAIITEAKLEVRNTRTKEVSEGLVDSSTGNYA
                     VAVANEKEKEDDFVMVVKKEDYSFTSALIEVNEEIFEAPLVIDFEIKPIEKGKSVELR
                     DINYATASYQIDKKSLLVLDEFIIFLNDNPNLKIEIRGHTDNIGSMQTNITLSNNRAK
                     AVYDYLIKHGVNPNRLQHKGYGPNLPIATNETEAGRAKNRRTEFYILDK"
     gene            complement(24132..24998)
                     /locus_tag="PKN53_04815"
     CDS             complement(24132..24998)
                     /locus_tag="PKN53_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HOD88310.1"
                     /translation="MIKKIKTYLSLVKFSHTIFAMPFALIGLFVAIQELGHFPDWYIF
                     VLIILCMILARNAAMAFNRYTDHKIDKANPRTAMREIPTNKVSPKNALIFTIINSILF
                     ITATFFINKTVFYLSPLALFIVLSYSYTKRFTFLSHFILGLALSIAPAGAFLAITSYL
                     TTQILMLSAAVLFWTAGFDILYSLQDEEFDRENGLFSIPAKCGRNNSLKLSSLIHLVA
                     ACFIILFSLSLNSNFLIITGTCIFLILLIFQHLIVKKDDISKINIAFATTNGVASLVF
                     AVFIIASLYYKA"
BASE COUNT         8012 a         3764 c         3919 g         9345 t
ORIGIN      
        1 ctatagttgt agtttgcaat taaatcaaat ttgaatggac tagattttgc aaaatacata
       61 tttaatatag gtgtaaaact atgttttgaa tcttctagtt taaaattata agcaccagta
      121 ataaaaaagt gtctagcatt ttttattaat ccagaaatgt ctgcgttaga accagagaaa
      181 aaataatttg atttgaataa ttgtaaaatc gaaaatccta caaaataatt attcgcattt
      241 aacaaaattc ctgcatttgc gtcaggtttc caaatttttt cgtcagtttc aaagttcaaa
      301 atttggtcat ttgtttcgta aactttaatt tgtgatgaca ataattttaa ttgagaaaaa
      361 ttcatatcca agccaaaact aatattaaag tttttagtgc gaattatgta tgaatagccg
      421 aggttaaacg caagtctttg actaatccca attctgtcat gaactaaagc agcagatgca
      481 cccatgtcgc ttctgaataa gtatttccca taaattgaat taattcgagg agcgccagaa
      541 aaacctgtcc attgtattct actatttaaa agtatttcgg gaatctcttt cattgcaatt
      601 gcagcaggat tttggttggc aatattaaat acataatttc tatggttacc aatttgctga
      661 gcagataatt tgaaactact tattaaaagt aaaataccta tatatgtaaa aatctttagt
      721 ttcattttta tctaattatt gtaatatgtc ctttaaatag tgatttgcca tcgcctaaat
      781 ctattatata ataataagtt ccttctggta aatctttacc tttaaatgtt ccaccccaat
      841 cattttggta attttctgta tcaaaaacta aatttccaat tctattgtat atccatattt
      901 tatttcttgg gaatttatca atattttcga ctatccaagt gtcatttgct ccatctccat
      961 ttggagttat tgtattataa atagttatgc aatatgttaa ttccattaaa tcaacattat
     1021 aaatatataa acagttgttt gcatcagtta tgcttatata ataatttcct tctctgagtt
     1081 cagaaatatt attttcttca gaattattat cccaaaggaa tgtgtattct ggagttcctc
     1141 cagatggatt aacttctatt gctccatcat atattccaga gcaagttgga ggagttaatt
     1201 taaaattagg attaagcgaa tcaggctcag aaattagcac tctaatagta tctgtacaat
     1261 tatatttatc gcttgcaaac acaaaatgtt cgcctgctgg taaacctttt gctgtatcgc
     1321 caatatgtcc gtttgaccaa ataaaatgta ttggttgttg tccgtcttca agttttgcaa
     1381 ttgctacagc aatactatca ttaaaacata agttaggttc tataacatta taactgccgt
     1441 aaatttttcc aagtttgtta acaaatacgg ttaagctatc cgaacaatta ttagcatctg
     1501 taactattac ttgattttga ccataaggta aatttgttgc agtttgagtt gtttcgttac
     1561 ttatccacaa ataggagtaa ggaggcgttc ctccttctgg cattacagtt ccagagccag
     1621 ccttaagtcc acattccaca ggatctgttt ttccggttaa ttgtaacatt gatggttggg
     1681 tgaatcttat gctatcaaaa tagtggcaag agtttgcatc aacaactgta atgttataat
     1741 atgtatttgc tggcattcct aggcctgtaa agtcttgttg ttcaacacct tcttcccaat
     1801 atactgtgta aggaggtgtt ccgcctgtag gaataacgtt tgcacttcca tcataatagc
     1861 caaaacaact tacattatta atttcagaaa agttagaagt aagatagttt gcaggttctg
     1921 ttattgtgta agtttgaatg tttttacaag cattttggtc tgtaactgta attgtatagc
     1981 taccaatagg caaattacca atagagtctg aaaaatatcc attgctccaa gtaatattat
     2041 aataaggtgt tccgccgctt acatcaacag taatatagcc gtcatgtccg cctttgcaac
     2101 taacattgtg aatttgttct accaatatta gttcgtcagg ttgagtaata tgtgtgcttg
     2161 ctgtgtcgga acagccattt gcatcaacaa ccgaaacaaa ataatatccg ggatgcatat
     2221 ttgtaataat gtgcgaactt tgagttgggt cgtggaacca atgataagta tatggttgag
     2281 ttccgccact tgcttgaaca actaaacttc cattgtctaa accaaaacaa ggaatatttg
     2341 atgaatttat gtttgcaatt agagtatcag gttcaataat agtaatgctt tgtgaccacg
     2401 aacaattatt tgcatctgtt gcggttatgg tataagttcc agcattcaaa ttatataaat
     2461 cttgatatgt ttgaccgcta ctccataaaa ctgtgtatgg tggagtagct ccagaaagtg
     2521 taatgtcaat agaaccgaag tctgttccgt ggcaaggtaa atcagtttta gtataatcaa
     2581 ttgtaatagg agaggtgggt tgactaatga aaatggagtc agtaacaaaa cattctctaa
     2641 agtctgaaat agttacataa taccagccag atgttaaatc tgttgggctt tgttggtggc
     2701 taccgtttga ccattcaaag aagtaaggaa atgttcctcc agagacttct gtagtaatgc
     2761 ttcctgtgaa aaatccataa caatctaaat cgcttgctgt taagctatga ataataggtg
     2821 gatttattgc tactgaaatt gaatcataac tttcgcaatt ttgagcatct gttatagtaa
     2881 tcatataata tcctgggttg ccataaatac tatctatatc ggcaaaagaa ttttgtgtta
     2941 actgtcctgt tgaccattga aattcataag gtgcaattcc attttgagct tgtgcataaa
     3001 tgtatccgtc agtttgtcca tagcaaaatg cttgctcggt gtaaagtaga gaatttactt
     3061 ctgatttatc gtatggacgt atataagttc ctgggaaaac tatatcctcg gcaaagtgca
     3121 ttaattccca agtttgatca aaccaagtaa caggaacata acccaaactt actgtccatt
     3181 caaaaagagg tccaatatcc caaggtccta tatctttttc taatccttga ataaattctt
     3241 catcttcgta gtatccttgc atagcaattg gttcggaaac tattggtttt cctatgttaa
     3301 taggtagttc aaaactaggc attacattgt ctgcaatttg agatttgaac ggagttacta
     3361 tagtgattga agcgctaaga gcttctataa caaacgagaa tgcaatacta tcccaagtgt
     3421 ggtttcggat agttggttga atattatatt ttacaccaac atacatagaa tcccagtcgt
     3481 ggcacgggta agtgatagta aggtcgtttc caacagccat agtaattgta tcgccaaaac
     3541 cactttcaac ttctgcattt gagttttgag ggtcggtaac aatataaggc atttcaacag
     3601 gaaaatctaa ggttagccaa attgtggggc agaagtaaat atcttggtgt aaagtgtttt
     3661 caatattaag ataaacactt agcaaagaat attcaatttg aaaaattata tctcctaatg
     3721 gtgtgtttat tggatattga agcgtgtcgt ttaaaaaagc tatagcttgt tgtatttgtg
     3781 gtccttctgg tggtggaata tagccagcca tcacatataa aaaacctaaa acatcgagtt
     3841 ctactaacat gtaagggtct tcaccttttg caattaagca atttgaacca ggctcaatat
     3901 agtcttcagt aaatacttca ggcaaatcca catgagcttc aaagttgaaa cccacaagat
     3961 ctgtaaaatc tatatcaata ggcagttcgt agtcgtggca aaaagtaaat tggtcgccaa
     4021 catagcaagg ataaacacag tattgggttt ctgcatttat atgaaataaa gtatctaatg
     4081 tgtaaggtaa agaaacactc gctggtatta aatgtattgt gtcgcaagaa aatacgcaaa
     4141 ctacaatatt cataaatggg tcgaactgat attcaaaatc aagagtgatc actccaactg
     4201 gagggaaaga tgtttctaaa gcccagccat cagttacttc ataagatgtg tgtattgttg
     4261 caggtccacc ataattgaag ctaaaatctt caggaaactc taaagttgtc tttattggat
     4321 aatctaagct aaaacttcct gtataaaagc catgtgcttc aaaagtagaa cgtaataaag
     4381 caataattcc gacattcatt ccaactccaa actgttgacc taagaactct gaaatttgag
     4441 aaaatcccca agattcatta atgtcaatat caaaaaatgt atgatcgaca tctatcccat
     4501 aagcataatc aggtccccac atattttggt tgtgagattg atagagcatt tcgtgttgca
     4561 aagtattttc ttgcccaaaa atgtttaaac ttaaaagtat aaacaaaaat aaatatgata
     4621 ttttaacgaa atgcttcata ttattcaatt gagattaaac atgcttcaat agctggaaat
     4681 aaaagttttt tcatatttag agggtaatta aaatttaccc aaatagtatt ttcttcgcca
     4741 gtaaaaaaca tagctctttc gtaaaccatt tcaccactag taaattctac gaaaaatatt
     4801 acagcatctt tgcctgtagc agtttgtaaa tttacttttt ctttaaatat atagttttga
     4861 gattcaatat attcttttgt cattgaagat gaaatgtctc tgtacgaaac tccatttatt
     4921 tccataactt gtattgttgc agcagaacct ttgtgaacaa atccgtttat gctttcatct
     4981 acttcaaaat attctggagg aatcattttt acatttgtat ttggagcaaa aactagcttg
     5041 ttgggattgt attctagctt ggcattttgt tcttgaacaa aaacttctaa ttcatcggtt
     5101 tgtgaaaaaa ctgatagtga gctaaaaatt aaaactataa ttattaataa tttgttcata
     5161 acaaaacact taatttaata attaataaat gcaaatttaa acaaacttga tgaattacaa
     5221 aacatagttg caaaaattta tattttattc aacaaatatt tttttaacta aatttacttt
     5281 tgaattactg agttttacaa aataaattcc agacgataaa ttaaagttca tctcatcttc
     5341 atttgaattt atttgttttt cttcaataat ttgtgagtga atatttaata tctgtaaatt
     5401 gtactcttca ttttgatttg gtaaaagtat tttaaacttt ccagtatttg gatttggata
     5461 tatgtcaaaa tcaaagtttg tatcagtgtg taagccagtg aaaggactga tagttaaatt
     5521 aaagtagcaa gaagcagagc attgtgttgt ttcatcaata tatgtataaa ctatggtgta
     5581 gtctccgatt tcgtaatttg ctggatttat gtatgttatt tgatttccgt ttaaatagta
     5641 gtttccacct tcgggttctg ctccagataa aggaagtggt gagtcatttt cttcaagatt
     5701 aatatcattt ggacaaaata cttctggtgc ttgttttacc acaatttcaa aactgcattg
     5761 ttcgctttcg tatgagtaag taattatatt gatgcccaaa cttgcatttt caggataaaa
     5821 aactccatca acaactgcat ctccagaata tgtgccacca agtggagttg ctccttctaa
     5881 agcaaattca tttgcatttg agcaaatttc aaaattttcg gtacattcta caacattagg
     5941 attataaaca taaattgtga aagtgcaaga atccacaaaa ttattttcat cagtgtatat
     6001 atatgttatt aaataatttc ccacaccata ttcatttgga ttaaagcttg tgatcggatt
     6061 gttgttataa taatattgac cgctttgagg tgtagctaag ttaagttcta aagttgcatc
     6121 attgattgat aaatattgat tgtcgggaca gattatgctt tgaattaaaa caccatctga
     6181 agtagcaata tcggaatata gaccagcacc atttttagct ttaaccgaaa tataataagt
     6241 ttgaccaatt tcaagatttg catttgttat tgtaaagttt gtagcaagtc cattatttgt
     6301 ccaagttaag taatctgttc ctccttgact tgtgccaata gcataaaaat aagcacttat
     6361 tccagaattt gggtcttggc tttctgtcca attgccagat aaattttgtg aatcattgct
     6421 actatctaca tcttgagcta ttccatcatt aacatttgaa atacttgtag gtttgctaaa
     6481 atcaatattg tagttttttg tgattattgc agataggttt tttgcactat ccatgcacat
     6541 agagctaatt tttgctgcag caatatcagg attaggattt gaatatctta aatctccatc
     6601 tgttgcattg cagacaattt ctgtgttttg gttagaatat cttgaacgga aagttctaaa
     6661 attatcaatc gacattatag aatttccact tcggaaagaa atataacttc cagtaaaata
     6721 tggattagtg tctttatgtt cgccaataag tttattgtct tgatacacaa aaaattcacc
     6781 agtaactctg tcgtaaagga ttttaatatt ataccaagtt tctgccgcaa aattaattgt
     6841 aaaagtttta tctaatgaaa gtacattatc tgtaactcta taaaactcca aagattgtgt
     6901 gtcttgtagt ctaaaccaaa taaaatatcc gttacctcta ttgcttaatt ctggattgtc
     6961 gcagaaaaaa tggaagccag ctcttttgtt tacattactt ccttcaactt ttaaatcaaa
     7021 ttcgtacaaa tatttatttg ataactcttg atttaggtaa gcatatatgt ttgaatttcc
     7081 tacactttca tcattgtgaa ttaaaatacc ttctgagtta gtagtccaat tcccaacttg
     7141 attagtccaa tctgtgctta atgcattatt aaaatcatcg aaaaaatgtc ctttgttttt
     7201 gtttgctgtc cattttgttc catcaaaatc agaaatgttg aaaaatcctt tttctactcc
     7261 actaagttgg tcattgtcag agaaataagc agagaagttg tcggtgtgcc aagttccagt
     7321 aacttcaata tcagtttcag gattgtcaaa atcagtataa atattcttag actttgattc
     7381 agaccaattg ttggcattat ctataatttg tgttctaatt cttgcagctt cttgagtgtt
     7441 gtttggactt tgatatctaa tctctgcatt tggacttagt tgagttgtta tgatttcgct
     7501 tgcactacga gttttataca tttttatatc atcgtattcg catatgcttt gtccagttct
     7561 aagcgaaata gaattaccag attcaaatgg tgttgggtca gttatagatg taacaaactc
     7621 attatcaata tataatgaaa ttattcctgt ggttgtgttc agaataattt ttacgtcata
     7681 ccaaatgtta ggttgcaaat cataagattc aaagtgaaaa aatcctcctt cactagtgtc
     7741 gtatgaattg ttattatagc gataaatttc agctgttcct gaacctgtcc ctagtgtagg
     7801 gtcaactctt agataaagca tgtatgaagt ttgtcttccg ctagcttcgg ggtcgctaca
     7861 gaaaaagtgc attccagctc tacgattgtt gccagaacct gaaacttttt gtttccaatg
     7921 atataaataa gtctcgcctt gcacttggtt tacataagca taaagatttg tattggtact
     7981 tgtttggttt gtttgtttta ggtggttttc gcttattgac cattcaccag tatattctgt
     8041 ccattcggca tttatttctg tttcaaaatt atcattaaaa aaaccatgct ctccattggc
     8101 acgccactca gttccgtcaa aatccataac ttgatagaaa ctataattta cacctgaacc
     8161 gccttcattg tcttggtcgt taaaatttac ttcaaaatct gaattatacc attttctttg
     8221 agtttgaata tctgttgttg gtgcttgagt gtcaaaacta ccatcgcata tatctaagat
     8281 ttgttgtgtg gcagttctga ttgaaggcaa tagagcatac aaagcatcac cagggcaagt
     8341 agttccacca ggatttacat ctctatgtcc acaaattcta tgaacattta tatatccgct
     8401 gttatcttgt ttaattatac ttgccatact tgttgggtca aaccctctgt aatcgtacca
     8461 ccatgctaaa aattctgcac atttatctag ctgtggttga gttggagctg ttccaggaga
     8521 atcagagtct cctaagaaat ttaagcctat agaataagtg tttgaataac ctgcatgagc
     8581 gccgtgcaca tcttgattgg gtaagtttgg attatgccga ccttggaaaa aatttccata
     8641 tttgtcgaaa agataattat aaccaatatc gctccagcca agagtgttta catgatagtt
     8701 ccaatagctt ctaacaacgg ctgctccatc tgtgtatgag ctgggacttg caccatgatg
     8761 aattacagta tgggttggat ttctgtaaac aacagtatat gttgggtata ggcaattaga
     8821 aacgccatta caccacccag atcgagtaat gattggtgat ggaaattctg gacaagcttt
     8881 tgcaccagat tttgtacttt ttgtttctgt gtttgtgatt attgttggtc taacatttaa
     8941 taaaatcaag taaatttcac ttataatttc accttctggt gggtgaatat aaaattctat
     9001 agaatcatgt tcccactcgt ccaagccaaa cataaggttg gatttataat aatttgattt
     9061 tgtttcgttt ggtttagtaa agccatcgtc aatttcccaa tcagtccaac ctttattagg
     9121 tttgtgaact ttgtaaacaa tctcaaaact acctgccgaa taaatttctg tacttgatac
     9181 ccaacctaga gcaaaagatg taaattctac gggagttttt aaataaaaac cgtaaccacc
     9241 atttctgtct tggataattg gtgaagtaat tttgtaagta ttgatacttg catctttact
     9301 gttaaaagtt tctgttgttt caaactcaac ttgtccaaat gctactgcgg acaaaaagct
     9361 aaataaaaat gtaaaaattt tcctcataat tttaaataat caataattta acaatgtgtg
     9421 caagttacgg caaaatgttt tttaaaacaa atatttttaa atattttttt agctttgcaa
     9481 gaaaaaaaat ataattatgc ctaaattctt caaactttta aaattatatc ctaagaccta
     9541 ttggatttca aatgtgatgg agctttttga gcgttgggct tggtatggta tgtttatgct
     9601 ttttgctctt tacttgactg gctcaaccga tgcgggtgcg ttaggttttt cgcaaactca
     9661 aaagggaatg atgatgggaa cagttgttgg tttgctttat ctaatgccaa tttttactgg
     9721 tgctattgcg gatagatttg gatataaaaa agtgctgata atatcttata taattcttag
     9781 ttcatcgtat ttcgctcttt ctttggttac tagtttttat gcagtattta tagttttttt
     9841 aattcttgct gtgggtgcat ctttgtttaa acccgtaata tcagcttgta tagcaaaaac
     9901 cacaaacgac caaacttcat cattgggttt tggaatattt tatatgattg taaatatagg
     9961 tgcatttata ggtcctattg ttgcttcttt agcaagacaa cacgattgga aatatgtttt
    10021 ttatatttct tcggctgtaa ttttgataaa tttagtttta gtaattctat tctttaaaga
    10081 gccagaacga gaaatcaaac aaaataagtt ttcgcaagaa ttaaaaatta tttttaaaaa
    10141 tatttttgaa gcattaaaag acatgaagtt tgtagtgttc ttattgataa tagttggttt
    10201 ttggacaatg tacaatcagc tattttatac tttgccagtt ttcattgacc aatggatgga
    10261 cacttcaata gtttataata aattgcattc tgtttggccc tggttggcag aaacaattgg
    10321 tactcccgaa aaaactatag cagcagaaat gcttacaaat cttgatgcaa tgtatattat
    10381 aatttttcaa atagctgttt cttattttgt gatgaggctt aaacctattc attcaattat
    10441 tagtggtttt ttggttgcaa caattggttt atttcttacc ttttctacaa acaatccaat
    10501 gtttttattt gttagtattt taatttttgg tttaggcgaa atggcaggtt ctccaaaaat
    10561 tactgaatat attggaaaaa ttgctcctcc aaacaaaaca gctttgtata tgggttatag
    10621 ttttttacca atggctggcg gaaattatct tgcgggaatt ttatcgggat cggtttatca
    10681 aaaatattcc gataaaatta gcttattgaa aatggaagtt agcagtagag gtttgaatat
    10741 accagaaatt tcagaaaatt tttcgcaaac agattatata aatgcggctt gtcaacaaat
    10801 gaatatgagt caaggtcaat taacggagta tttgtggaca aattatcaac cttataatat
    10861 tgggataatt tttgcagcaa ttggtattgg tactgttata gcaatgttgt tgtataattg
    10921 gtatttgaat aggaggaaag cgtaattaca gttcttcttt caaatattta gctgtatagc
    10981 tatttttatt tttgataata tcttcaggtt ttccttgagc aataacagtt ccacctcttt
    11041 cgccaccttc tggtcccata tctataatcc agtcggcaga tttaattata tctaaattat
    11101 gttcaataat tatcatgcta ttgcctctgt caactaaaga gtttagcact ttaagaagca
    11161 cttttatatc ttcaaaatgc aaacctgttg taggctcgtc taaaatataa agagtatttc
    11221 ctgtttctcg tttcatcagt tctgttgcaa gttttactct ttgagcttct ccgcctgaga
    11281 gagtagtgct tgattgtcct aaattaatat atcccaagcc aacttgttgt agtacaatta
    11341 attttttata aatatgtgga atgttttcaa aaaattcaac agcagcgttt atagttaatt
    11401 caagtacatc agaaatagat tttcctttaa attttacttc taaagtttct ctattatatc
    11461 ttttgccacg gcaggtttcg cattgtacgt aaacatcggg tagaaaattc atttctatgg
    11521 ttttaacacc agcacctttg caagtttcac atctaccacc tttaatatta aatgaaaatc
    11581 tgccagcttt gtaagctcga atttttgctt ctggtaaact tgcaaataat tcacgaatat
    11641 ctgtaaacag tcctgtatat gttgcagggt ttgagcgtgg tgtccttcca ataggcgatt
    11701 ggtcaacttc gataaatttg tcaatatttt caattccttc tattttgtca tgatctaatg
    11761 gaaaagcgtt tgaattataa aaatgacgtg aaagtgcagg aactagagtt ctatttaaaa
    11821 tacttgattt tccacttcct gaaacaccag tcatgcaaat cattacacca agcgggattt
    11881 caaggtcaat gttttttaga ttatgacctt tagctccacg aattattatt tttttatcag
    11941 aaatttctct tctggatttt ggaacttcga ttttaagttt accatttata tattgagagg
    12001 taagagaatc tgattttagc atgtcgcttg gttttcctgc aaagactaaa tgtccacctt
    12061 tgcgtccagc gccaggaccg atatctgcaa caaaatcagc ttcagtaatc atttctttgt
    12121 cgtgttccac aactattata gaattgcttc tatctctaag ttgtttaagt gaatttatta
    12181 gtcttttgtt gtcgcgttgg tgtaagccaa tacttggttc gtctaaaata tataaaacat
    12241 ttactaaacg acttcctatt tgagttgcta atctaattct ttgagattcg cctcccgaca
    12301 aactgtttgc cgaacggttt agtgataaat aatctaatcc aacatcaagt aaaaaactta
    12361 ctctgctact taattcttta attacttctg ttgcaattaa tctttcttta ttatctaatt
    12421 tgtctaatac agtatctagg aatttactga ggtttaacat gtccatttct gcaagttcgg
    12481 caatattttt ttctgcaatt ttaaattgta gtgcttcagc ttgcagtcta gttccattac
    12541 attttggaca agtgataaac ttattgaatc taaatgatga tttgtttgag tatggatctt
    12601 tttcttcgtc ttcttgtttg gctttaattt gatttacaat gccatcaaaa cttatagaat
    12661 aatttgaagt tagtccaaga ggtgtatttt ttaattgtaa actttcggga tagccaaata
    12721 atacagcatc aattgcttgt tcgttaagtt catcgattgg agttttgagc gaaaagttta
    12781 atttttcggc aagggcttct agaatccaaa aaggtaaact atttttatat tttccaagaa
    12841 tgctaattcc gccattgtaa attgaaagcg atgggtctgg aataatttta tttatatcaa
    12901 tatcggctat tactccaagt ccgttacatt ttggacaagc accttgtggc gagttgaaag
    12961 aaaaagtgta gggagcaggt tctctgtatg aaattccgct tgttggacac attaaaaatc
    13021 gactaaaaaa acgaggtttg tcgctatctt tttctaaaac cataattata tttttgccta
    13081 aactcattgc taggtctata gttttcttta gtctatttct atcttgattt ttaataatta
    13141 atttatcaac tactaattct acaaagtggg ttttgtatct atctaatctt aaattataag
    13201 taatttctct aatttctccg tcaattcttg cgtttagaaa accttttttt cttaggtttt
    13261 caaaagtttc tttgtagtgt ccttttcttc cgtcaacaat tggtgcaagt atataaaccg
    13321 ctttgtcttt aaaatctttt aaaataatat ttataatttg ttcgtcgctg tatttaatca
    13381 ttttttcgcc tgtaacgtgc gagtatgctg ttgaggctct agcataaagc agacgtagat
    13441 aatcatatat tcctgtaatt gtgccgacag ttgagcgtgg attttttatt gttgattttt
    13501 gctctatagc aatcactggg cttagtcctg ttatactatc aacgtctgga cgctccatgc
    13561 tgccaataaa acttctagca taagcagaaa aagtttccat atatctgcgt tgtccttcgg
    13621 cataaattgt gtcgaaagct aacgaagatt ttccacttcc actcaaacct gtgataactg
    13681 atagttgatg ttgtggaata tctaggctta aattttttaa attatgagtt cttgctcctt
    13741 caactgaaat aaaattctct ttcttattca tcattgtttt tactttgttt catacattca
    13801 cgtgcgttct ttgcaggtaa aacaattttg tattcctttt tgtttgggtt aattaatgac
    13861 tttccagtta accagggatt aaaatattta agcattttgt agtttgtgcc gtatttgaaa
    13921 gcaaattcca ctaaatcaga aatggtagta tcaacaacaa tttcttgagt tttaatttct
    13981 ttatatagat gtttttcatc tatatcaaag ccataaagtt taggattaga cataattaat
    14041 tttaatgcta atatcctata cacatatcta gcagtttctt gattcaaatg taaatcccaa
    14101 taactattaa ctttttgata atcaatatct ttttgtaagc cacccattcc catattgtat
    14161 gaagctgctg ccaatgtcca gcttttaaaa actttatatg ctttgtgtaa atattctgtt
    14221 gctgcaattg cagatttagt atgattgtat ctttcgtcta taccttttcc tacatgcata
    14281 ttgtattctc ctgctgttgt tttcataaat tgccaaaatc ctgtagcacc agctggacta
    14341 acaacatttg ctaagccact ttcggcaaca cataagtatt tcatatcatc aggaatacct
    14401 ttttctttta atattttttc tatctcagga aaatatcgat ttgctctttt tatataaatt
    14461 aatgtagatg aatgtctgta agtatttaca ataagttcgt aatctaaagc ttcgtaaaca
    14521 tcaaagtttt gtagaggaac attttcacca caaaaactta aagtgtcagg aatatatggt
    14581 tgaaaaactt tatatggtat tgttgttggt tggatttctg tttctttgat ttgatttgct
    14641 ttttccgagc ttgaaaaaat aaaactatta gcaaaaataa gacctaagcc aagtcctagt
    14701 ataataaaaa aaggtatttt cataagtttt ttcttgtcca tatttttttg ttatttttct
    14761 ttacttactt tagtgtttat gtataatagt aaaaacacag tgatggggta taagaaaaag
    14821 agccaaagtt gtgaatatgc aatagtagaa cttttttgta tgagacaaaa tgctaatgct
    14881 aaacctatag agtttaacac aaaaaataca agtgctatta tatctgttgg caaacctaaa
    14941 tagtataagt gatgcgtagt atgatctttg cctcctataa aaggagattt ttttcttaat
    15001 agtctattta tagttactgt tgttgtatca gttattagta ctaaaaacgc taaaaataca
    15061 attaaaaact gcattgtatt aaatccatat gaaaattcag ttatagggat agcattccag
    15121 aaatatttta ttcctgccca tgctaataat attcctaaaa attgagatcc attatctccc
    15181 atatacattt ttgatggatg ccaattataa aataaaaagc taagtaaaga tgctattgta
    15241 gaaactaata ttattaaact taatatagaa ttttcacctc cattagctat caagtatatt
    15301 gcaacagcaa taaaaattga aagacttgat gctgacgaaa caccgtccat attgtccaac
    15361 atgtttatag agttcattat tccgactacc cacaatatgg ttattagata attcatccag
    15421 tccattgggc ttataatgat gtaaataccg ttgtaaatta atattagagc acatacaatc
    15481 tgtattataa atttgaatat tggcgatgtg tttaaaatat catcggctaa acccattaaa
    15541 aatgataatg ttacaactaa aaaaaggcta atatattctt ttgaaaagta tatgttttct
    15601 tttaaaaata aaatcactat catggttaac aagaaaacaa aataaaaact tagtccacca
    15661 aatattggtt ttgattgtga gctaaatctt atagcagatt tgttagattt ctttactaaa
    15721 aatttcaaag gacttttaat taaaaagtta tttaaataaa gactaaaaag aaaagaaaca
    15781 ataaatgtta ttattataat gtaattcatt ttttattttt ttttctaaac ttattaaaaa
    15841 attttggttt agttatttct tcttcgccgt aatatgaatt gtgggtgttg ttgagataac
    15901 taattccata ataagcggtt ttttttgtat agccataacc tgctttatat ccgtatccgt
    15961 agcttgcaat acttttttct ataccattaa gtactatagc taaatttttt aattgaattg
    16021 atttagagaa atagtttaaa ttatttataa atgctcttgg gctatatcca gctctcatca
    16081 catatagttg attatcagct aagctatatg aagtaagggc atcattgact attccaatag
    16141 gtggggtgtc tataataata atatcataat tttgttttgc ttgctcaatt aacatattat
    16201 acttgtctga aagaataaat tctcctgggt ttggaggcaa aggtcctgac gtaataaaat
    16261 ctaaaccttc taaagaactg ttgttaatac attcttggaa actattttta ccaagtaaaa
    16321 tgcttgtaac tcctttaata ttttcgacat taaagcattg attaagttta ggctttctta
    16381 aatcaaggtc taagataata actttttttc ccgacattgc ataaatagct cctatattta
    16441 gtgctaaaaa tgtttttcct tctcctgcaa tcgttgatga aactgttatt attcttgatt
    16501 tatcttttat tgggtaaaat tctaaattag tccttatatt tctaaaagct tcgcttagca
    16561 tagatttggg acgtaaatga acaaccattt gggaaacatc aaagtttgtt ttgctaagtg
    16621 gaatgccacc aacaaccgga atgtttgtat gattagtaat atcatcaata gatagaattt
    16681 tattataaag taaatattta attgctaaga aaataaaaga taaaactaaa gaaattaaaa
    16741 aagcaattgt cattattgtt gaaaatatag gaaagtcggg tgctgaagga atatttgctt
    16801 tttctaaaat tatattgttt gaaacatagc cagcttgtga tattaaagtt tctgcttttg
    16861 atttaattag ttgtgcataa aaatcttcgt taatagcata tagcctttga agtcgtgcat
    16921 attcgatttc atcatactta gattctttaa aggctatgtt ttcaagttct ttatttttct
    16981 tgatgagttc agttttttct gccttaaggc gatttattgt tgagttggta aaatcaatta
    17041 ttgtctcttt tttagatttt atttgttcat ctatggtttg aattttgtgg ctattagacg
    17101 ttaggtcaaa taaaagaatt tcttttttat ttaataattc ttgtagagaa ttaatcatcg
    17161 cagacaaaaa tgcttcagac ttttgtcctg aaagcattgc taaaagttcg taagtattaa
    17221 tatctggatt tgaacgtaat ttatctaaca ttttctctaa agtcataatc tcaaaatcaa
    17281 catttaaaag cttagactct atatcagata tttgcgatga ataaatattt gttttattaa
    17341 aaatatattc atcgcttatg tttgtaatat tattttgttc tctaaagtta tgcaaatctt
    17401 tttcgttagc attaagttgg gtaaaaacat tttccatttg ttggtcaatg tattttataa
    17461 tgttggttgc agattctttc tttttggctt catcaaactc aataaatttt tcggctattg
    17521 tattggtaat ttctgctgct tttctagcat taaagtcttg ataagttatt ttaatagttc
    17581 cggcagtttc gttaagtatt tgaacattaa gccgttttga taagtttttt aatattgtat
    17641 tagggttgtt tatgatgaaa aaatatagag atttattttg tggactaagt tcaagttcta
    17701 aatctttttg cgatgggaaa tgaacataaa tttccattcc ataaatagtt tgccattctt
    17761 ctggttggat tgtgtattca tatttttctg ttcctaaagt ataggaaatc aggcatttat
    17821 agtctttaaa ctctaaatca atattttggt cacatattac attatttgta atattatatt
    17881 taatttcaaa aggtgtggag ccatacaatt ctgttgacaa aaatgttcct tttctaaaat
    17941 aactaatatc aagtggaagt tctgcaacaa ctgtttttaa aaattcgttg gagcgtatta
    18001 gttctattac tggtgctaga tctttgtcca ctaaattaga agaaatgcct aaaatttggt
    18061 ttgttttatt ttcgtttttt acttgaatta tagttgatgt tttaaaaata ggggttgaat
    18121 atctatgaaa agcaaagcca gttcctaagc atattaaaat caatagaaca actacccaaa
    18181 tatttttttt aactataatt aaaaatgttc ttgtgtcaaa cttttgatta agtatagatg
    18241 ttttattttc gtccattttt attataattg cttaagttta aaaaatactc cataaattgt
    18301 aactaatgtt gtaattagtg ttaagtatgg tgagacttct cttaatactc ttgtgaagta
    18361 ttgtggtctg ctatcgatgt aaataatatc gttagcttct agcaaaatat ttgaactttt
    18421 tagttcttta atatttgaaa tattccaata aaaaacttcg gggttttgtg ttaaattgcc
    18481 acgaattaat tttattctat atgaacggct aatatccgta actcctccaa cttgtgctat
    18541 tgcttcaatt agggtaaaat tatcattcgg catttcaaca acacttgctt tggttccgcc
    18601 gtccataaaa atcattactt ttctattaat aacttgtact aaaacaaaag gttcgataaa
    18661 atagttgcta tattcttttt caagaagcaa ttctgcttct cttattgttt tgccagcaat
    18721 atcaattctt cctaatattg gtagttttat tttaccatca aattctacaa gaaattcaga
    18781 cccgtgttga tttctttggt aattagcatt tgcattagca ttttccatac tacctgtccc
    18841 aaaaaaagat tcgcctgtat tactaacaac tcttaaacct attttatcga aaggttggat
    18901 aatatattca ttttttgaaa cttcaaattc agattttgga taatctttat cggtcttaaa
    18961 catatctgag ggtctgagaa gacggcaaga atttccaaaa aatattgtta atattaaact
    19021 tgatataaat aaaatcttta ttttcatgta gttttattaa attcaattat gcaaaaataa
    19081 ataaatttct catattcctt ttaatttgta gctaattttt gctttttaac tctgatttta
    19141 ttaaactcaa attgttgaat aaaatttttt tataaattta tattgttaaa tctagttttt
    19201 atatttgtaa ataattcttg tattgatgaa acgattgttt ataatattta ttatttgctt
    19261 tggttggaat attgtttatt ctcagagcta caattcttat tatcatcaat ttgagatgcg
    19321 cgcaaattat ggtctaacta ttccgcacca tacttgctta aaatatttaa tagaaaaaaa
    19381 tatttatata ggcgaaataa cttattctat aaaaacagat ggctcagaac cttggcaaca
    19441 tgtatggcgt tttccagaat tgggtgttgg atatttaatg ggtagtcttg gtaatatcaa
    19501 tgtatttgga ttttcacaat ctttatatgc tttttatggc gtgcctattg tcgaaactgg
    19561 taattttatt tttaaatatc gctttggttg tggcttggct tatgttagcg aaaaatttga
    19621 cgctagagca aattatttta atattgcttt tggttcatat ttgaatgcac atctgcattt
    19681 ttcgctttta gttgattata aatttccaga tatacctttg tatctaactg caggatttgc
    19741 ttataatcat ttttcaagtg gagcaatcga aaccccaaat ttaggtttaa atcaagttac
    19801 tggtaatttt ggaatcaaat atatgtattc tatgtataat tattctttac ctaaacgcac
    19861 tgtgccttat cagtatgata aagattttga gttttcggca tattatgccg tttcggttaa
    19921 agaaaattct acttatgaaa ataaaaagta tatcattaat tctctaattt gtgattttgc
    19981 ttctcgctta aataatcaaa gaagtttagg tgttggatta aatttaattt ttgacccatc
    20041 aattaaacct ttattagaag aagattatac tggtgcccac aatttgctta gagttggtgt
    20101 tcatgctatg caagaagttt atttgaccga tgatttgagt gtgattatgc acttgggaac
    20161 ttatgctttt aataaatata aagatgggaa aaaaatattg gtttattcaa aactcggtgt
    20221 tagatatact tttgctgaaa gatattttgc caatatagtt ttaaaaacac atacaacaac
    20281 tgccgattat attgaatttg gcttaggatt tagaattaac taattttcta aaacatgaat
    20341 ttcaactctt ctattttgac tttgttccca agagttttgt tcacgtgggt atagtttttg
    20401 acttgaacct acgcctttat aaatcattct tgaagcctca atacctcctt ttcttaaaaa
    20461 aataaaaact gctttagctc tatttagact taaagtataa gtgccgtcag tatgatcgta
    20521 accatcaggt tcggattcat cttggcaaca aatgtgtcca ataatttcta tttttaaatt
    20581 tggattctca cgcattagtt gtaatagttc cattaggggt tcttcggctg tgggcaagaa
    20641 aaatggtgta ccacctttaa aatttatatt tttgatatta aatttatttc cttttactaa
    20701 atcttctgct ttaatatttg ctaagtctgt atgttctgtt tttttgtcaa attttgtttc
    20761 gaaacttata gtgtcttggt aagtgtagct tttagtttgt tcttcaacgt taaatgtaat
    20821 aataatttca gattttctat gttctttaat tcctctgtcg ctttcatttg tacgattctc
    20881 aaaactacta ttttcatgta cagactttcc ttgacaagtg attattttat cagaattgaa
    20941 gcctaaattt attaaaaatg ttttagtcgc atttgctctt tgttgagaaa gcttatcatt
    21001 aagttcttta cttcctaaga aatctgtata agcatatatt tcaattttat caattttaga
    21061 taagtcaact ttatcaaact catgtttata cttgttttca attttataac tatttatatc
    21121 aaaatatatg taaaagtttt tggcatcttg agctttaact tcaaaactta ttaagcaaat
    21181 tgcaattaaa aaaacaatac tttttgacat cataattttt aaatttgtta tttatttaac
    21241 tgtaaaatta aaattttatt attgatttga ctgtgttttt tagaaaaaat agaggtttta
    21301 ttgatgattt ttattttttt ataaattaaa gtacaaagta ttcgtttgtt aaagtggtgg
    21361 ttagtagggg tttagagttt gtttttgttg agcaaaatta tgttcagata aaaaaaataa
    21421 aatatttata aaaaaatgag cgtaattcaa aaaaaatgac taatattgca atccaaaaat
    21481 tgtaaggaat atttaaagtt ttttgtaatg gaattaatga atatcgtaaa tcagaaaatg
    21541 gctattgaaa aggaatttcc taagttcaaa gctggagaca caataactgt tagttataaa
    21601 attcgtgaag gaaataaaga acgtattcag gagttcagag gagttgtttt acaaatcaat
    21661 ggtgaaggtc gcacagctac ttttactgtt cgtaaaatgt ctggggcttt tggtgttgaa
    21721 agaatttttc catttgcgtc gccttttatt gatgctataa ctgttaataa atatggacga
    21781 gttcgtcgcg caagaatatt ttatttacgt ggacttacag gtaaaaaagc tagaattaaa
    21841 gaaagaagat tttaagatta gtttagttta tattgctcat aaaagcgaga ctgctcaatt
    21901 aattttgggc agtctttttt ttgctaaaaa atcttgtttt gactatttat tatttgtcta
    21961 aaatataaaa ttctgttcta cgattttttg ctctacctgc ttcggtttcg ttagtagcaa
    22021 taggcaagtt tggtccatag cctttatgtt gcaatctatt tggatttact ccatgtttta
    22081 ttaaataatc atatacggct ttggctctat tattcgatag agttatgttt gtttgcatac
    22141 ttcctatatt atcggtatgt cctcgaattt caatttttaa attaggattg tcgttcaaaa
    22201 aaataataaa ctcatctagc acaagaagcg actttttatc tatttgatac gaagcagttg
    22261 cataatttat atcacgaagt tcgacagatt ttcctttttc tataggtttt atttcaaaat
    22321 caataactaa tggagcttca aaaatttctt cattaacctc aattagagct gaagtaaaac
    22381 tataatcttc tttttttaca accattacaa agtcgtcttc tttttctttt tcgtttgcaa
    22441 ctgctacagc ataattccca gtagaagagt ccacgagccc ttccgaaact tctttagttc
    22501 tagtatttct tacctctaat ttcgcttctg taataattgc acccacttca tcaagaagtt
    22561 gtcctttaac tatgaatact ttctcgggtc tagcttctgg atataattca aaagaataaa
    22621 tatcccagcc accttttcct tttaatttat tgctagcaaa atacgctttt gtgccttgag
    22681 tatttacaac aaatcctaaa tcatgtgctt gtgtatttat tgggtatcca agatttactg
    22741 gaatactcca ttcattattt ctatatttag aaaagaaaat atcatatcca cccaatcctt
    22801 tgtgtccgtc cgaagaaaaa tacaaagtct gactatctga atgaataaat ggagattttt
    22861 cgtttccagg ggtatttatt gttggtccca tattttgggc tttattccat gttccgtctt
    22921 cgtttttagt tgaataccaa atatcgctag taggattgtt tgggtcgaaa ccaatattgc
    22981 cttctctaat acttgaaaaa tatagagttt ttccgtctgc agatattgat ggttgagact
    23041 cccatgtgtt taatccatta atgttaggtc caagattaat taattcagac cagccatttg
    23101 ctttcctaac agaataataa atgtcgcagt tatcataagt tctggaaaca aattcacata
    23161 tagtaacata aagtgtagag ttatctattg ttaaagcaga agctccttgg ttttttccag
    23221 tattaaaagg gtagggcata ggctcgcctt tcgaaaacct tgtaccagat tcatctaaag
    23281 ctcttgcaac agaaaactct tctgtatatt tttcagtagt tacgctagca atatcttttt
    23341 tcatgtaagc atgtgtaaaa aatgctaaac ttccgtctgg cgaaattaat ggtaaaaaat
    23401 catcattaac tgttgaaata ccttcaacaa taacaggttt gaaaggaact ggattagata
    23461 ttaattcaag ataagtatca atataatcta aaataagttc agcttctttt atgtgtttgc
    23521 tttctttagg agcgtttata gtgtataagt caaggtattt ttttgcattt tcgtaatctt
    23581 ctttttcata aaacattttt cctaaagtat aatttacaga ccaattgttg taatgaggac
    23641 aggtagaggc tactttttct aaacttcttg cataatttgc ataaaactta ttttgcgatt
    23701 tagcgtcttt ggaatttttg tatttaatat aattaatttc agctactcca agccaagctt
    23761 ctgtaaactc gggttcttca tctacaattt ttgacaaaag taattcggct tgatcaaact
    23821 tttctgagtt tatatattca attgatttgt taaaatcttt aattattttt ttatcgtcta
    23881 tttcgttaca atagtcataa agagattgtg aaaatgaaaa agaatatgat atacataaaa
    23941 gtaatagcaa tatgtgtctt tttattttca ttaaaaactg tttacaacaa agatatttta
    24001 aaatatccat ttttaataat taaaacgaaa taatattgtt aaaattatat tgttagcaaa
    24061 taatattcca tttgagcata attttagaat aaataaatga aaattttagt aaagttcttt
    24121 taattagtct attatgcttt gtagtagagc gaagcaataa taaaaactgc gaatactaaa
    24181 gatgcgactc catttgtagt tgcgaaagct atatttattt ttgaaatatc atcttttttt
    24241 acaattaagt gttgaaaaat aagcaaaatt aagaatatac aggttccagt aattattaaa
    24301 aaattagagt ttaagcttaa actaaataaa attataaaac atgctgcaac taaatgaatt
    24361 aggctagata gcttcagaga gttatttctt ccacatttag caggaattga aaataaacca
    24421 ttttcgcggt caaattcttc gtcttgtaac gagtataaaa tatcaaagcc agcagtccaa
    24481 aagagaacag cagcagatag catgagtatt tgagttgtta aatatgaagt tattgcaaga
    24541 aaagctcctg caggggctat tgataatgct aatccaagaa taaagtgaga taaaaaagta
    24601 aatctttttg tgtagctata tgataaaaca ataaataaag ctaaaggcga taagtaaaag
    24661 acagttttat taatgaaaaa agtagcagtg ataaaaagta ttgaatttat tatcgtaaaa
    24721 ataagtgcat tttttggaga gactttgttt gttggaattt ctctcattgc agtgcgagga
    24781 ttagctttat ctattttatg gtcagtatag cgattaaaag ccattgctgc attacgagct
    24841 aaaatcatac ataaaatgat taatacaaaa atataccaat ctgggaaatg tccaagttct
    24901 tgaattgcaa caaataagcc gattagtgca aacggcatag caaagattgt atgagaaaat
    24961 tttactaggg atagatatgt tttaattttt ttgatcattt tacaaaattg caaaaaaaaa
    25021 cggagttaaa agctccgttt
//
LOCUS       DAOPNX010000030        24791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_2549_length_24791_cov_6.506428, whole genome shotgun sequence.
ACCESSION   DAOPNX010000030 DAOPNX010000000
VERSION     DAOPNX010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24791
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2549_length_24791_cov_6.506428"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="PKN53_04820"
     CDS             complement(<1..1310)
                     /locus_tag="PKN53_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOD88311.1"
                     /translation="MSSQKRDGFTSKFGVIAAAAGSAVGLGNLWRFPYITGENGGGAF
                     LLVYIFFVVLIGIPVMMSEFVIGRRSGSNAIGAFRKLAPGTAWWLVGLMGIIAAFVIL
                     AFYGTVAGWTLEYIIQAFQNSFVGKSQAELSTDFNNFTASTFRPLLWQIVVMILTAAI
                     VLRGVSKGIEKATKILMPLLIVLILVVCIRSITLPGSSKGLEFLFKPDFSKLTFKAVL
                     MALGQAAFSLSIGMGALITYGSYVRKDTPLLSTSISVAVADTVIAILCGIMVFPALFA
                     LGGESTGGPGLVFVTLPGVFQSMPGGYIFGILFFILVFLAAITSTISLLEVVVAYFKE
                     DLRISRFKATVLATVAITVLGVFCTLSFGKLSHIKLFDKTIFGLADYVSANILLTFGA
                     LFIVIFTGWYLGKSKFIEEVKLGCKVNPIVLNVIVFIIKWIAPVA"
     gene            complement(1409..2212)
                     /gene="ispE"
                     /locus_tag="PKN53_04825"
     CDS             complement(1409..2212)
                     /gene="ispE"
                     /locus_tag="PKN53_04825"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOD88312.1"
                     /translation="MISFPNSKINIGLNIVSKRDDGYHNIETVFYPIPYKDILEILPS
                     QKLEVINLGKKIDCKIEDNLCYKAYQILNSEFNLPPVKIILYKNVVFGSGLGSGSSDA
                     AHTLISLNKLFDLNLDEEQLQFYASKLGADCAFFIKNKAVFAQGLGNVFSEISLDLSG
                     KHLMLCLPDVHVSTALAYKNCKPNMPSQSLKSLISLPLEQWKNNIKNDFEENLFVQFP
                     NLQKIKHELYQKGAIYAQMSGSGSAIFGIFDSLPNLNFKNCDEIKTFIL"
     gene            2343..3647
                     /gene="der"
                     /locus_tag="PKN53_04830"
     CDS             2343..3647
                     /gene="der"
                     /locus_tag="PKN53_04830"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOD88313.1"
                     /translation="MGNIVAIVGRPNVGKSTLFNRLIERREAIVDNVSGVTRDRLYGH
                     CEWNGRTFSVIDTGGYVKDSDDIFEKEIARQVVIAIEEADVIIFMVDVLAGLTDLDSS
                     VADLLRKSEKPVFVTANKVDIHERILDAYEFYNLGLGEIYSISAINGSGTGDLLDEIV
                     KVLPEDKNAEEEELPRITIVGRPNVGKSSLTNVLVGEERNIVTDIAGTTRDAINTRYN
                     KFGFDFMLVDTAGVRKKSKVNQDLEFYSVMRAIRAIENSDVCLLMIDASVGFEAQDQN
                     ILNLIRKNKKGIVILVNKWDLIDKTTNTLKEYQKSIMEKIAPFNDIPILFISALTKQR
                     VHKVIETAMAVYENRTQRIPTARLNEVMLQAIEDYNPPSVKGKYIKIKYVTQLPTPTP
                     VFAFFANLPQYIKEPYKRYLENKLRENFNFTGVPIVLVFRNK"
     gene            3696..4715
                     /gene="pheS"
                     /locus_tag="PKN53_04835"
     CDS             3696..4715
                     /gene="pheS"
                     /locus_tag="PKN53_04835"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOD88314.1"
                     /translation="MIDKINQILSEVEAFKPKSIEEIESFRLTYLSKKGKISTLFDDF
                     RNVPVEQKKEVGVKINILKQTATEKLNQLKEQFIVDNSGKIDIDLSRPGDPLKLGARH
                     PLSVVHNEIVEIFTKLGFSISDGPEIEDDNHNFALLNFPENHPARDMQDTFFISNDMN
                     VLLRTHTSSVQVHEMLEQKPPIRTLSPGRVYRNEAISARAHCLFHQVEGLCIDTNVSF
                     ADLKYTLEIFAKEMFGKHTKIRLRPSYFPFTEPSAEVDVSCSLCGGKGCNVCKNTGWL
                     EILGCGMVDTNVLEATGIDPKIWSGYAFGMGIERISMLKYEIKDLRLFFENDIRFLNQ
                     FTSAF"
     gene            4719..5522
                     /locus_tag="PKN53_04840"
     CDS             4719..5522
                     /locus_tag="PKN53_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOD88315.1"
                     /translation="MILNHLKIGNNIKNDNLVILHGLYGSGETWTNVANLLSDKFNIY
                     LPDARNHGKSFHNPSHTYPELVEDLKIWTDSLNIEKFNLLGHSMGGKTAMFFANKYPQ
                     YINKLIVADISPRNYSNISQHVPSINFHLNLISNMLKLELKKYKSYSEVAKELVNYEE
                     NIRNIILKNLIKKDGKMCWKLNLQAIANNFPEIVQGLDTDEFIEKKILLPTLFLKGDL
                     SNYITNQDFKLIEFIFPNSKVETVENAGHWLHIEQPQEVSNKIFNFLID"
     gene            5525..6907
                     /gene="fumC"
                     /locus_tag="PKN53_04845"
     CDS             5525..6907
                     /gene="fumC"
                     /locus_tag="PKN53_04845"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOD88316.1"
                     /translation="MTKFRQEKDSMGIVDVPADKYWGAQTQRSFQNFKIGGKMPLEVI
                     YAMAILKKSCAKANHNIGVLSEEKAKAIANVCDEICQGKLDDQFPLVVFQTGSGTQTN
                     MNLNEVIANRAHVLNGGSLTDSKKLLHPNDDVNKSQSSNDTFPSAMNIAAKTVIVNKT
                     LPALKRLHKEFVKLSKEFANKIKTGRTHMMDATPISFGQEFSGYAAQLEYGINALEKS
                     LDHLSELAIGGTAVGTGLNAPKGYDKEVCKYLTEFSKIEFKTAPNKFEALAAHDSILE
                     THSALKQIAVAINKIANDFRMMASGPRCGLNEILIPENEPGSSIMPGKVNPTQIEAIT
                     MVCAQIIGNDLSITTSAMNGHFELNVFRPVMIKNFLESANILAEACDSFLNNCVSGVK
                     INDKQTEEYLKKSLMTVTALNTAIGYDKAAEIAKNAHKKGISLKESAMEIGSISEKDF
                     DNIVDPRKMI"
     gene            6977..8839
                     /gene="asnB"
                     /locus_tag="PKN53_04850"
     CDS             6977..8839
                     /gene="asnB"
                     /locus_tag="PKN53_04850"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOD88317.1"
                     /translation="MCGITGFYTKDNPNFVSKDELELMTSCLAHRGPNARGVFYTDSI
                     GLGHQRLSILDLSDDANQPMESHRMRYICVYNGEVYNFKEIAKDLNIELKTNTDTEVI
                     IEAFAEWGITFINKLIGMFAIAIYDKQEKILYVFRDRMGIKPLYYYWDGKNFAFGSEI
                     KALLQVKQIREERQLNNFAIYEFLHLGYIPEPNTIYTNIRKFPSGQIGIVSQNGFRLE
                     SYWDIEGCIRRNVITDFHEAKERVHSLIKSSVSMTLLSDVPFGTFLSGGIDSSLVTAI
                     AAKTHSQKIDTFSLGFNEPKFDESKYAKQIADYLGTNHHQYIVSVDDVKSIILDMLDF
                     FDEPFADSSAIPVMLISKFATQKVGVVLSGDGGDELFHGYGAYTWAKRLDKFPYKNFR
                     NVFATILELSPSNRSKRASYLFKYKNKDTLKSHIFSQEQYFFSEKELRKILNPDLIFD
                     NSLEQDYSNFVRKLTPEEAQAIFDLKYYLKDDLLVKIDRASMRYGLEVRVPLLDHRIV
                     ELALNLDPKLKNSKNIPKFLLKEILYDYLPREFFDRPKSGFAMPLKTWLNTDLAFLIK
                     DYLNESVINEFNIIEFSEVQKLITEFKLGKDYLYNRLWLLIILHKFLIKNKYSQ"
     gene            8849..9226
                     /gene="crcB"
                     /locus_tag="PKN53_04855"
     CDS             8849..9226
                     /gene="crcB"
                     /locus_tag="PKN53_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOD88318.1"
                     /translation="MLKHAIYIFIGSGLGGIIRFGLGKWISSIHSTNFPLGTFIVNIL
                     ACFIVGFVIGLADSKQIISASAKMFWVVGFCGGFSTFSTFSNETLNLLQTGNHTTSFL
                     YIALSVIVCLTFTFLGLLIANKV"
     gene            complement(9242..11002)
                     /locus_tag="PKN53_04860"
     CDS             complement(9242..11002)
                     /locus_tag="PKN53_04860"
                     /inference="COORDINATES: protein motif:HMM:NF019436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD88319.1"
                     /translation="MKNYLIFILIVLCASQAIYSQNKLPYPVIFVHGLTGSEKTFATS
                     MEFLRDSYQLGDINVFDAVLNADNDLSTALLENDVKWENFVFNDKVIYLGPRNYTDNK
                     DDYVQEWTGSSIFAINFKEENIKGASGFFYSYFDQSNQSAIYKQGYALGKMIQEVLDF
                     TGADKVVLVGHSMGGLCIREYLQRTDENGVHVNWIDPTVADGHKVARVATFGTPHLGS
                     NTSPDPTKSDIPDVQGKSEANRDLIWEYDSYTFCNTYPQGIYLFGGNENCIASEGGFF
                     GNSTFYNVDVNCNGSETDDIIGINEGPDSQSYNPEMPLPLNIKYTYMTSIWLGWDIGL
                     VGDGAVDINKQWLYDQDRNPVPLGITDTLLTDVIHTSEGSDYYTLIRGLDEPNQFSLA
                     YTLEPDNEIIGFVTFQQNGVEIDEDVFKLKSGNNNAIAFVFNGESSGVNTIQFYDLNE
                     NLIQSKTITNFIDTTIINIPANDDGIYVKLIGTASSTTWENPYKIHFIPYVTAEMPNY
                     NNASVSIFPNPSNGICEFSLMNISDCYIKIFNTNGVEVFRSEITNQKELDLSFLSNGI
                     YTIKFDFDSGSLIQKLVIIR"
     gene            11138..>11909
                     /locus_tag="PKN53_04865"
     CDS             11138..>11909
                     /locus_tag="PKN53_04865"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HOD88320.1"
                     /translation="MNKSPLEILVSKFEKDGELIRINEFVNPELEITEFADRMMKLPK
                     GGKALFFENTGTDFPLLINHFGSEKRIFSILGIKNWEEPSQKIDLLFKNIINPPQTFS
                     EKLSALSELKKIKKWMPKKVKSKGKCQEVIVENPDLSKLPILKCWKHDGGRFITLPLV
                     ISEDPETKIRNIGMYRMQVFDNKLTGMHWHLHKGGAAHFEKHKKLNKKMPISVVLGGD
                     PILTYCASAPLPQDIDEFILAGFLKNKNVKLVKSITNDI"
     assembly_gap    11910..12009
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12010..12705
                     /locus_tag="PKN53_04870"
     CDS             <12010..12705
                     /locus_tag="PKN53_04870"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HOD88321.1"
                     /translation="IRLPDYGVAHNLVLVKAKATYKGQAIKIMNALLGAGQMMFSKII
                     ICFNENIDIQDDKQVFETLLKVNDLKNNTIFSKGPADVLDHSAYTYAFGGKILIDATN
                     PQNEKLENREIKLIKNSEFDKTKKLTGINIIIDKPEAIKDNHTALWLILNNIDPIYDV
                     KIEDDFILINASSKNLKENNFQRKWPNPVCADKKTIEKVDKIWCKYTNMDFVESPSLK
                     YIDLVSGDAAVYE"
     gene            12741..13391
                     /locus_tag="PKN53_04875"
     CDS             12741..13391
                     /locus_tag="PKN53_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88322.1"
                     /translation="MEYNQAFNDLINNGYEVKTKQYLNEGLELFKKYSNGFFGFYLII
                     FGVAAIASAPPLMYVGSVLSLLQAPLIAGFFLVANDVIRGNTPKFADFFNGFNFFLPL
                     VLLNIVSSIFIAVGLILLIVPGMYLAVSYSFANMFIIFFNYDFWTAMELSRKIITKNW
                     WSFFGFLLILFLINFAGILACGVGVLFTLPITSCMLFVAFEDIVGSAMRNQQQTNF"
     gene            13404..14171
                     /locus_tag="PKN53_04880"
     CDS             13404..14171
                     /locus_tag="PKN53_04880"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HOD88323.1"
                     /translation="MKSIIKFSVLILLSLFIYFNTYSQDCTTILTPSVKKSETKLCHT
                     HYCFVYSEKHEQSKWVAYILKDYMTESNAKRTDKFMTDPLVATGTANDNDYKNSGYDK
                     GHLLPAADMSFDSIAMVETFYFSNMSPQAPSFNRGIWKNLETYVRTTSSKLGCIYVVT
                     GPVLENDLENIGENKVSVPKYFYKTILYHSDTLTSGIAFLIPNEKATDKDIFSYTLSI
                     RDLEKTTKLNFWHTLPRSMQNKVEKNTDFDFWKQFAK"
     gene            14191..>14290
                     /locus_tag="PKN53_04885"
     CDS             14191..>14290
                     /locus_tag="PKN53_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOD88324.1"
                     /translation="MDKTVLIAFSLTLIAGLSTGIGSLISFFAKKTN"
     assembly_gap    14291..14390
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14391..14738
                     /locus_tag="PKN53_04890"
     CDS             <14391..14738
                     /locus_tag="PKN53_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOD88325.1"
                     /translation="GIPVALAIAIHNIPEGIAVAVPIYYATGSKSKAMRLSFLSGLAE
                     PLGAIIAYLIIMPFINDVVLGISFAITAGIMVFISFDELLPGAQKYGNHHIPTYGVVI
                     GMAIMAFSLLILN"
     gene            complement(14735..16300)
                     /locus_tag="PKN53_04895"
     CDS             complement(14735..16300)
                     /locus_tag="PKN53_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOD88326.1"
                     /translation="MSLINSIVSLINYNRLAEIDYFRSHPFEVQEKVLAELLEKGAFT
                     EIGKKYNLDKVKNGVDFSNKMPLHDYDSLKPYIDETMHGKENILWPGKTKWFSKSSGT
                     TSDRSKFIPISKEALEDCHFRSGKDIFMLYADRYPESKVFVGKTLSIGGSTDINANDS
                     DSHYGDLSAILIKNLPFWTYFNRVPKSDIALIDDWEVKLEKIVETTYNKNVTCLVGIP
                     TWFLVLIKKVLSYTGKSNILEVWPNLELFVHGAVNFSPYQETFRNLIPNPAMIYMETY
                     NASEGFFAIQDETSSNPLLNNEGLMLMLDYGIYYEFIELSDYLAGRNNAVPLEGVECG
                     VNYAMVITTNAGLWRYIIGDTVSFVSKNPYKLKITGRTKHFVNAFGEEVIMDNAQVAL
                     NHACEITGAQVREFTVAATFYPQDFTSEGAHQWLFEFEQKPNNLQTFVQALDSKLREV
                     NSDYDAKRFNDLALAMPEIIVLDEGSFFAWMGRRGKLGGQHKVPRLSNTREYVDDLLK
                     HSKVIEKIVNLKD"
     gene            16348..16842
                     /locus_tag="PKN53_04900"
     CDS             16348..16842
                     /locus_tag="PKN53_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOD88327.1"
                     /translation="MFFIDIILIIIFIYFIYKGVKNGLIKELASLLALTLGIFLAIKF
                     YAKLIPILEDKASSYAEYLPIFAFAIIFIATVVVVFMFSHLLNRFIKLIKLQWLNKTG
                     GAVFAILKISLIIGGLLFFINRLNEKAGLFDPAIWDKSILYKFIIKIFNIIFPYTKEL
                     TNLF"
     gene            16901..17731
                     /locus_tag="PKN53_04905"
     CDS             16901..17731
                     /locus_tag="PKN53_04905"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOD88328.1"
                     /translation="MDKKRNKISNWFRKSFQLVVYHGKTYDTIRTIKFNRLGFTFFIF
                     GIIVVLFAILSVFVIYTPIKQMIPGYPDKETKKLIYENAIKTDSLMHELEMKDQYLKM
                     IQDLVFNEVPIDEDFVVPVQNLSEQQIIDFNNPTKPRIKIEDDTDFVVPEEEFPNIVE
                     PIKGIVVSKYSPSQNHFGIDIAAISDEVIKATYRGTVISSAYTIENGYTIIIQHKSNL
                     ISVYKHAKSSLVKEGERVATGQIIANYGDSGENSSGPHLHFELWKNGKSINPENYIQF
                     "
     gene            17731..18897
                     /locus_tag="PKN53_04910"
     CDS             17731..18897
                     /locus_tag="PKN53_04910"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOD88329.1"
                     /translation="MKKQKNIAILGSTGSIGTQALKIISDYPDKFQAEVLVANNNVDL
                     LIKQAIEFQPNIVVIANEEHYEKLKNAINHLPIKVFAGAKSIEQVVQMDCVDIVLTAM
                     VGFAGLLPTIKAIEANKTIALSNKETLVVAGHIINKLLENSKSQIIPVDSEHSAIFQC
                     LIGENHKNVDKLILTASGGPFRGKDIDFLKKVKPKQALKHPNWNMGSKVTIDSASLMN
                     KGLELIEAHWLFGIDAKNIEVVIHPQSVIHSLVEFVDGSLKAQLGVPNMRIPIQYALD
                     FPNRSVSNKHRFSFKDFENLTFEQPDLKTFRNLQIAIDCLNAGGTSPCVMNAANEVAV
                     DLFLQEKISFVEISDLIEEGLATIEFINNPSLKDCVEIDNITRKKTFELYKKIK"
     gene            18897..20198
                     /gene="rseP"
                     /locus_tag="PKN53_04915"
     CDS             18897..20198
                     /gene="rseP"
                     /locus_tag="PKN53_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOD88330.1"
                     /translation="MGGLIMAGQLLLSLTLLVVIHELGHFLAARMFGIKVTKFFIFFD
                     VGFKIWSKKIGDTEFGFGWLPLGGYVKIAGMIDESMDTEQMKQEPKEWEFRSKPAWQR
                     LIVMLAGVFMNVIAGILILSISHMVYTKEYLPVKNVEQGIYAYPYARYLGFETGDKII
                     AIDGKPTQRYEDVFSSKLFFAKSITVDRYGQTKEIAIPDTLYSYLKNGGLFISLDNFN
                     FSIDSCVDNMPISDLNLKKGSKILSINRERIKSFGQLKEILHYNKNRELSFVFDNNGK
                     IDSLDIFIDSTAKLGIYASLPKFTGAKYNFGTALLYGWKDAFETVNANIKGYGLIFAG
                     KEKVRESLQGPIGIAKVYGAVWDWAKFWRLTGIISMILAFINVLPIPALDGGHALFLV
                     IEVITGRKLSDKFMGIVQVIGMLILLALMVFVIGNDIINLF"
     gene            20232..21053
                     /locus_tag="PKN53_04920"
     CDS             20232..21053
                     /locus_tag="PKN53_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88331.1"
                     /translation="MRKIIFVFLVLNSSFLSLNAQEFLDFSNTAPSEKRILLVPFDPR
                     IYVNDATAIMLKNERSNHDELMQYFRYQFNLQLHNAMMDSCSVVSLFSDNTRIDQEDI
                     NTLYSLISYELVLATKNAPENPEENKKKSFFAKKKEEKELQRRLEETKEYDARIHNGE
                     IVSRRQTTQDMSLNIVFHQPEVLEEIANRRNVDLFLFINQFEIVGNYGDPYLSGNTKA
                     ERNLKVHFSIYNTKGQMIHNSFGITKLPFNLDDKQTVVELYFPEVIRQIIHNISF"
     gene            21056..21652
                     /locus_tag="PKN53_04925"
     CDS             21056..21652
                     /locus_tag="PKN53_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HOD88332.1"
                     /translation="MNTNFIFATNNPNKLKEVRHIANNKFNIVGIDEFGITEELPETQ
                     NTLEGNALQKARYLYEKISKNCFADDTGLEVEALNGRPGIFSARYAGEEKSSIDNIKK
                     LLNELKDCSNRSAQFRTVVALILNGKEYLFEGIIKGKIIGELKGENGFGYDPVFVPLG
                     FEKTFAELPLNIKNTISHRAQAFEKLFNFLKHYEVDLK"
     gene            21698..24067
                     /locus_tag="PKN53_04930"
     CDS             21698..24067
                     /locus_tag="PKN53_04930"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOD88333.1"
                     /translation="MNKINYILSIVCLMILSFFSTAQVAVGQWRDHLPYNHGTSLAIS
                     ENEIYMSTNAGLMRFDKRTSELEKLSKINGFSDAGVSTVKYSKKFDTFIIAYTNGNID
                     LVQNNTITNIGDLKRKFINGDKTIYDISLNGDFAYLACGFGIIELDLKRKEIKETWYI
                     GENGTYVKINSLDNDGEYIYAATDIGIYKGNFNNKLVDFSNWEIITQQNLPNDFKWLE
                     GKEFSSLTIFDGKIIANYHNPTQDDADTLVVYYNGNWERLYPEENMFEAVTSSSEHLI
                     LINRYWIRLLNKNFEEVKTVWHYTINEQYRQAIPADAFLEDEKILWIADKKNGLVRTP
                     EPWDYKSYIINGPETEKVYDFAVSESKIIGINGGMTAAWGPKWAHGMFFEFKDEFWTS
                     ALDKPNNGLEGVFDLTTITINPNDPNNIFIGSWVNGLLEYRNNAYYKIHNDENSSLRQ
                     ALGNSGYIRIGGADFDSEGNLWVTNSLATPQIHVLDKQGKWTGINYSSIINDRNIGKI
                     LITKDNIKWVIVGQSGGLLVFDDNGTPDVRSDDKVKRLDIVNENGELISNDVYSIAED
                     KNGYIWVGTRKGVAVYYNPQDVFTSNKFTARQIKIPRNDGTDNADILLGNDVVTTITV
                     DGSNKKWFGTQSGGVYYTSADGLEIILHFNTENSPLISNNIICTEIIPNTGEVFFGTE
                     KGIISYRNIPTEGDDNFDKVYTFPNPVKPDYRGPITISGLVAGSYVKITDISGNLVFE
                     TRSEGGQAVWYGEDLRGNRVRTGVYLVFAANETGSKKNVTKILFISGNE"
     gene            24070..>24791
                     /gene="recO"
                     /locus_tag="PKN53_04935"
     CDS             24070..>24791
                     /gene="recO"
                     /locus_tag="PKN53_04935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOD88334.1"
                     /translation="MQKTEAIVLNKIKYSDSSFIVNLISEDLGRFAAMVRVSKSSKSN
                     IKQASFFPLNIIETEINLKNNREVQTLTYCNNVENLQNILLDINKLTIAQFIAEIILK
                     TIKEKETNSKTYNYIKNFTLELNSNSSETNNIHLLFLKDFAALSGFEMYNNYCKETPF
                     FNIKEGLFSPVFTSSTESLDLHESQILFNLLSAKNTNELIKLNSNNKRLLIEKILLFY
                     EHHIPNFGKTKSLKILQEVFND"
BASE COUNT         9191 a         3755 c         3781 g         7864 t
ORIGIN      
        1 gcaacaggtg ctatccactt aattataaaa acaattacat ttaaaacaat aggattaact
       61 ttacaaccta atttgacttc ttcaataaat ttagattttc ctagatacca acctgtaaaa
      121 ataactataa ataaagctcc aaatgtcaat aaaatatttg ctgacacata atctgctaaa
      181 ccaaagatag ttttatcaaa caacttgata tgcgataatt ttccaaatga taaagtacaa
      241 aatactccta aaacagttat agcgacagtt gcaagtactg ttgccttaaa tctagaaatt
      301 cttaaatctt ctttaaaata tgcaacaaca acttctaaaa gagaaattgt gctagttatg
      361 gcagctaaaa atactaaaat aaaaaataaa attccaaaaa tataaccacc tggcatagat
      421 tgaaaaactc ctggcaaagt aacaaacact aaaccaggtc ctccagtact ttctccacct
      481 aaagcaaaca aagctggaaa aaccataata ccacataaaa tagcaatcac agtgtctgct
      541 acggcaacac taatagaagt acttaacaaa ggcgtgtctt ttctaacata cgaaccataa
      601 gtaattaaag cccccattcc tatacttagt gagaaagctg cttgtcctaa agccattaaa
      661 acagctttaa atgtaagttt actaaaatcg ggcttaaata aaaactccaa accttttgaa
      721 gatcctggca aagttatact tcttatacaa acaacaagta ttaaaactat taataaaggc
      781 ataagtattt tagttgcttt ttctattccc ttgctcacac cacgcaaaac aattgctgca
      841 gttaatatca taactactat ttgccataat agaggacgaa aagtgcttgc tgtaaaatta
      901 ttaaaatcag tgctcaactc agcttgagat ttgccaacaa aagaattttg aaaagcttga
      961 ataatatatt ctaaagtcca accagcaact gtgccataaa acgccaaaat tacaaaagct
     1021 gcaataattc ccattaaacc cacaagccac catgctgttc ctggagcaag tttacgaaac
     1081 gcaccaattg cattagaacc agaacgccta ccgataacaa actccgacat catcacagga
     1141 atgccgataa gaactacaaa gaaaatataa actaataaaa aagcacctcc gccattttca
     1201 cctgttatat aagggaaacg ccacaagttt cctaaaccta ctgcagaacc agccgccgca
     1261 gctataactc caaatttact ggtaaaacca tctctttttt gacttgacat aataaataaa
     1321 ttttagaact taatttcaat tattacctaa acatcttaca ataaatcaat ccttaaatct
     1381 tcaaaaatat aaaaatttat caactttatc ataaaataaa tgtttttatt tcatcacaat
     1441 ttttaaaatt taagtttggc aagctatcaa aaattccaaa aattgctgaa cctgaacccg
     1501 acatttgagc ataaatagct cctttttgat aaagttcatg ttttattttt tgcaaatttg
     1561 ggaattgaac aaacaaattc tcttcaaaat catttttaat attgttcttc cattgctcta
     1621 atggcaaact aatcaaagat ttaagacttt gacttggcat attcggctta cagttcttgt
     1681 aagccaaagc tgtacttaca tgcacatctg gtaagcataa catcaaatgt tttccactca
     1741 aatccaaaga aatttccgaa aacacattac ctaaaccttg tgcaaaaaca gctttatttt
     1801 taataaaaaa tgcacagtca gctcctaatt tagatgcgta aaattgtaat tgttcttcat
     1861 ctaaattcaa atcaaataat ttgtttaacg aaattaaagt atgagcagca tcggaagaac
     1921 ctgaacctaa gccacttcca aaaacaacgt ttttatacaa aataatttta acaggaggta
     1981 aattaaattc cgaatttaaa atttgataag ccttataaca caaattgtct tcgattttgc
     2041 aatcaatttt tttacctaaa ttaattactt caagcttttg agatggaaga atttccaaaa
     2101 tatctttata aggaattgga taaaacaccg tttctatatt gtggtagcca tcgtcacgct
     2161 ttgaaacgat attcaatcca atattaattt tactatttgg aaaactaatc attctacaaa
     2221 attaaaatta atttcttaaa aacataattt ctaaaggttt ttatatttaa tagttttgaa
     2281 acaatgataa taaagctaat gcaataattt taattttttt atctttgtga aaattttgaa
     2341 ttatgggaaa catagttgca attgtaggtc gtccaaatgt tggaaaatca actctcttca
     2401 ataggcttat cgaaagacgt gaagctattg ttgataacgt tagtggtgtt acaagagata
     2461 gactttacgg acattgcgaa tggaatggaa gaacattttc ggtgatagac actggaggat
     2521 atgtaaaaga ttctgatgat atttttgaaa aagaaattgc tcgccaagtt gttattgcaa
     2581 tagaagaagc tgatgtaata atttttatgg ttgacgtgct agctgggctt acagatttag
     2641 attcttctgt tgcagattta ttaagaaaat cagaaaaacc tgtatttgta actgcaaata
     2701 aagttgatat acacgaaaga atacttgacg cttatgagtt ttacaactta ggtttaggcg
     2761 aaatttattc tatttctgca attaatggct caggtacagg cgatctttta gatgaaattg
     2821 ttaaagtttt acccgaagat aaaaatgctg aagaagaaga attgcctcgc ataacaatag
     2881 ttggtcgtcc aaatgtcgga aaatcatcat taacaaatgt gcttgttggc gaagaaagaa
     2941 atattgttac agatattgca ggaactacac gtgacgctat aaacacaaga tataataaat
     3001 ttggatttga ctttatgcta gttgacacag caggagttag aaaaaaatct aaagtaaacc
     3061 aagatttaga gttttattcc gtaatgcgag caattagagc aatcgaaaat tcagatgttt
     3121 gccttttaat gatagatgct agcgtaggtt tcgaagctca agaccaaaat attttaaact
     3181 taattagaaa aaataaaaaa ggaatagtaa ttttagttaa taagtgggat ttaattgata
     3241 aaacaactaa tactttgaaa gaatatcaaa aatctataat ggaaaaaatt gctcctttta
     3301 atgatattcc aattttattt atttctgctt taacaaaaca aagagttcac aaagtaattg
     3361 aaacagcaat ggcggtttac gaaaatcgta ctcaacgcat tccaactgct agactaaacg
     3421 aagttatgtt acaagcaatc gaagattata atccaccgtc agtaaaaggc aagtatataa
     3481 aaattaaata tgtaactcaa ctgccaaccc ctaccccagt atttgcattt tttgccaact
     3541 tacctcaata tattaaagag ccatataaaa gatatttaga aaacaaactt agagaaaact
     3601 ttaattttac tggagttcca atagtactag tttttagaaa taaataatta tattagtctc
     3661 gaaaatttat aaatttgcat tttaatttta aaaaaatgat tgataaaata aaccaaatac
     3721 tttcagaagt tgaagctttt aagccaaaaa gtatagaaga aattgaaagc tttagattaa
     3781 catatttgtc aaaaaaagga aaaatttcta ctctattcga tgatttcaga aacgtgccag
     3841 tagaacaaaa gaaagaagtt ggagtaaaaa ttaatatttt aaaacaaact gcgactgaaa
     3901 agttaaatca actgaaagag caatttattg ttgacaattc gggcaaaatt gacattgatt
     3961 taagtcgtcc tggcgacccc ttgaaacttg gtgcaagaca tcccctatct gtggttcata
     4021 acgaaattgt tgaaattttt acaaaattag gtttttctat ttcagacgga ccagaaattg
     4081 aagatgataa tcataatttt gctttactaa atttcccaga aaatcaccct gcacgcgaca
     4141 tgcaagacac atttttcatt tctaacgata tgaatgttct tcttcgtact catacctcat
     4201 cggttcaagt tcacgaaatg ttagaacaaa aaccaccaat tcgtactttg tcgccgggta
     4261 gagtttacag aaacgaagct atttctgcac gcgcccattg tttgtttcat caagttgaag
     4321 gactatgtat tgatacaaat gtttcgtttg cagaccttaa atatacctta gaaatttttg
     4381 ctaaagaaat gtttggtaaa cacacaaaaa ttagattgcg tccatcatat tttccattta
     4441 cagaaccatc agcagaagtc gatgtgagct gtagcctttg cggaggaaaa ggttgcaatg
     4501 tttgcaaaaa tactggctgg ttagaaattt taggttgcgg aatggttgac acaaatgttt
     4561 tagaggcaac tggaattgac ccaaaaatat ggtcgggcta tgcttttgga atgggaatag
     4621 aaagaatttc aatgctaaaa tatgaaataa aagatttaag attatttttt gaaaacgata
     4681 ttagattttt aaaccaattt acatctgcat tttaaaaaat gattttaaac cacttaaaaa
     4741 ttggcaataa tattaaaaac gacaatcttg taattttaca cggactttat ggcagtggcg
     4801 aaacttggac aaatgttgca aatttattaa gcgataagtt taatatttat ttacctgacg
     4861 caagaaatca cggaaaatca tttcataatc catctcatac atatcctgaa ctcgtagaag
     4921 atttaaaaat ttggacagat tctctgaata tcgaaaaatt taatctttta ggtcattcca
     4981 tgggtggaaa aactgccatg ttttttgcaa ataaatatcc acaatacata aacaaactaa
     5041 ttgttgcaga tatttcacca cgaaattatt ccaacataag ccaacatgta ccaagcataa
     5101 acttccattt gaatttaatt tcaaacatgc ttaaacttga actaaaaaaa tacaaatcct
     5161 atagtgaagt cgccaaagaa ttagtaaatt acgaagaaaa tattagaaat attattctaa
     5221 aaaatttaat aaaaaaagat ggtaaaatgt gttggaaatt aaatctgcaa gcaattgcaa
     5281 ataattttcc agaaattgta caaggcttag atacagatga atttattgaa aaaaaaatac
     5341 ttttaccaac acttttttta aaaggagatt tatcaaatta tatcactaat caagatttta
     5401 aacttataga atttattttt cctaattcaa aagtcgaaac agttgaaaat gctggacatt
     5461 ggttacatat tgaacagcca caagaagttt caaacaaaat atttaatttt ttaatcgatt
     5521 aatcatgaca aaatttagac aagaaaaaga cagtatggga atagttgatg ttcctgccga
     5581 caaatactgg ggagcacaaa cacagcgttc gtttcaaaac tttaaaattg gtggtaaaat
     5641 gcctttagaa gtaatttatg ctatggcaat tttaaaaaaa tcttgtgcaa aagctaacca
     5701 caacataggt gttttaagcg aagaaaaagc caaagcaata gctaatgttt gtgatgaaat
     5761 ttgtcaagga aaacttgacg accaattccc tttagtagtt tttcaaactg gttcaggcac
     5821 tcaaacaaat atgaacttaa acgaagttat tgcaaaccgt gctcatgttc taaatggagg
     5881 aagtttaaca gattcaaaaa aattactaca cccaaacgat gacgttaaca aaagtcaatc
     5941 atcaaacgac acttttcctt cggcaatgaa tattgctgca aaaactgtaa ttgtaaacaa
     6001 aaccctacct gctctaaaaa gattgcacaa agaatttgta aaactatcta aggaatttgc
     6061 gaataaaatt aaaacaggca gaacccatat gatggatgcg acaccaataa gttttggaca
     6121 agaattttcg ggatatgctg cacaattaga atatggcatt aatgctttag aaaaaagttt
     6181 agaccacctt tctgagctgg caataggcgg aacagcagta ggaacaggtt taaatgcacc
     6241 aaagggttac gacaaagaag tttgcaaata tctaactgaa ttttctaaaa tagaatttaa
     6301 aactgcccca aataaatttg aagctttagc tgcacatgat tctattttag aaacacattc
     6361 agcattaaaa caaattgcag ttgcaataaa taaaattgca aacgatttta gaatgatggc
     6421 aagtggtcca agatgtggtc taaacgaaat tcttattcca gaaaacgaac caggttcttc
     6481 aattatgcca gggaaagtta atccaactca aattgaagca ataacaatgg tttgtgcaca
     6541 aatcattgga aatgatttaa gcataacaac ttctgcaatg aatgggcatt ttgaactaaa
     6601 tgtatttaga cctgtaatga ttaagaactt tttagaatct gcaaatattt tagccgaagc
     6661 atgcgactca ttcttaaaca attgcgtttc tggagttaaa attaatgaca aacaaacaga
     6721 agaatattta aagaaatctt taatgactgt aactgctctc aacacagcta ttggatacga
     6781 caaagcagcg gaaattgcaa aaaacgctca taaaaaagga ataagcctaa aagaatcagc
     6841 aatggaaatt ggtagtatat cagaaaaaga cttcgataat atagttgacc cacgcaaaat
     6901 gatataattt tataaatatt aactggaatg caatgaaaat tatattaatt ttgattttta
     6961 aaatttttga aaatagatgt gtggaataac aggtttttac accaaagata atccgaattt
     7021 tgtaagtaaa gatgaacttg aacttatgac atcttgcttg gcacacagag gaccaaacgc
     7081 aagaggtgtc ttttataccg actctattgg actaggacat caaaggctta gtattttaga
     7141 cctttccgat gatgcaaacc aaccaatgga atcgcatcgt atgagatata tttgtgttta
     7201 taatggtgaa gtttataatt ttaaagaaat tgctaaggac ttaaatatag aactaaaaac
     7261 caatacagat actgaagtta ttatcgaagc ttttgcagaa tggggaatca cattcataaa
     7321 taagctaatt ggaatgtttg ctattgcaat ttatgacaag caagagaaaa ttttatatgt
     7381 atttagagat agaatgggaa taaaaccatt gtactattac tgggacggta aaaattttgc
     7441 ttttggatca gaaatcaaag ctttattaca agtaaaacaa attcgcgaag aaagacaact
     7501 aaataatttt gcaatctacg aatttttaca tttaggctat attccagaac ctaatacaat
     7561 atacactaac attagaaaat ttcctagtgg acaaattgga attgtttcac aaaacggctt
     7621 tcgattagaa tcctactggg atattgaagg ctgcattaga cgaaatgtaa taaccgattt
     7681 tcacgaagct aaagaaagag ttcactcact aataaaatca tctgtttcaa tgactttgct
     7741 tagcgatgtg ccatttggaa catttttgag tggaggaata gactctagtc ttgttactgc
     7801 aattgctgca aaaactcatt cacaaaaaat agacactttt tctcttggtt ttaacgagcc
     7861 taaattcgac gaatcgaaat acgcaaaaca aattgcagat tatttaggaa caaatcatca
     7921 tcaatacata gtttctgtag atgacgtaaa aagtatcata cttgatatgc ttgacttttt
     7981 tgatgagcct tttgcagact cttcagcgat tccagtaatg cttatatcta aattcgcaac
     8041 acaaaaggtt ggagttgttt tgtcgggaga tggtggcgat gagcttttcc acggatatgg
     8101 agcatataca tgggcaaaaa gacttgacaa attcccatat aaaaacttta gaaatgtttt
     8161 tgctacaatt ttagaacttt caccaagcaa cagaagcaaa agagcttcgt acctttttaa
     8221 atataaaaat aaagacactc taaaatcgca tattttttca caagaacaat acttttttag
     8281 cgaaaaagaa cttagaaaaa ttttaaaccc tgacctaatc tttgacaaca gcttagaaca
     8341 agattattca aattttgtaa gaaaacttac acccgaagaa gctcaagcaa tttttgattt
     8401 aaaatactac ttaaaagatg acttgctagt caaaatagac agagcatcaa tgcgttatgg
     8461 attagaagtg agagttcctt tgctagacca cagaattgtt gaacttgctt taaatttaga
     8521 tcctaaatta aaaaactcga aaaatattcc taagttttta ttaaaagaaa ttttatacga
     8581 ttatcttcca agagaatttt tcgacagacc taaatctgga tttgcaatgc cactaaaaac
     8641 atggcttaac acagatttag catttttaat aaaagattac ttaaacgaaa gtgttataaa
     8701 cgaatttaat attattgagt tttccgaagt tcaaaaactt attacagaat tcaagttagg
     8761 aaaagactac ctttacaata ggttatggct cttaataatt ttacacaaat ttcttatcaa
     8821 aaacaagtat agccaataga ttacaaacat gctgaaacat gcaatttata tttttatagg
     8881 cagcggacta ggcggaatta taagatttgg actcggaaaa tggataagtt caatacactc
     8941 aacaaatttc cctctcggaa catttatagt aaatatttta gcctgtttta ttgtaggttt
     9001 tgtaattggc ttagcagata gtaaacaaat tatttcagca tctgctaaaa tgttttgggt
     9061 tgtaggcttt tgtggtggat ttagcacatt ttcaacattt agtaacgaaa ctctcaatct
     9121 tttgcaaaca ggaaatcaca caacaagttt tttatacatt gctctaagcg taatagtttg
     9181 cttaacattt acttttctag gactactaat tgcgaataaa gtttaaagct gataaaattt
     9241 ctcaacgtat aatcactaat ttttgaatta aactacctga atcaaaatca aattttatag
     9301 tataaatacc attagacaaa aaacttaaat ctaattcttt ctggtttgtt atttctgagc
     9361 gaaaaacttc aacaccatta gtgttaaata ttttgatata acaatctgag atattcatta
     9421 agctaaattc gcatatccca tttgaaggat ttggaaaaat gcttactgaa gcattattgt
     9481 aatttggcat ttctgctgtt acataaggta taaagtgaat tttatatgga ttttcccaag
     9541 ttgttgaact tgctgttcca attagcttta cataaatacc gtcatcattt gcaggaatat
     9601 taataatagt agtatcaata aaattagtaa ttgttttaga ctgaattaga ttttcgttta
     9661 aatcataaaa ttgtattgtg tttacacctg aagattctcc attaaaaaca aaggctattg
     9721 cattattatt gccagattta agtttaaata cgtcttcgtc aatttctact ccattttgtt
     9781 gaaaagtaac aaaacctatt atttcattat caggttctaa agtataagca agcgaaaact
     9841 gattaggttc gtcaagccca cggataagtg tgtaataatc actaccttcg cttgtgtgaa
     9901 taacatcggt aagtaatgta tctgtaattc ctaaaggtac aggatttcta tcttggtcgt
     9961 agagccattg cttgttaata tccactgctc catcacctac taaacctata tcccaaccaa
    10021 gccatataga tgtcatatat gtatatttga tattcagtgg cagaggcatt tctggattat
    10081 atgactggct atctggtcct tcatttattc caattatgtc atcagtttca cttccattac
    10141 aatttacatc aacattataa aaagttgagt ttccgaaaaa ccctccttca cttgcaatac
    10201 aattctcatt tcctccaaat aaataaattc cctgtggata tgtattgcaa aaagtatagg
    10261 aatcgtattc ccaaattaaa tctcgatttg cttcagattt gccttgcaca tcaggaatgt
    10321 cagattttgt tgggtcagga gatgtattag aacctaaatg cggagtgcca aatgttgcta
    10381 ctcttgctac tttatgtcca tctgcaactg tagggtcaat ccaatttaca tgaacaccat
    10441 tttcgtcagt tctttgcaaa tattctctga tacataatcc acccatagaa tgacctacta
    10501 aaacaacttt gtcagcacct gtaaaatcaa gaacttcttg tatcatttta cctagagcat
    10561 aaccttgttt atatattgca gattgattac tttgatcaaa ataactataa aaaaatccgc
    10621 tggctccttt tatattctct tccttgaaat ttattgcaaa aatgctggac cctgtccatt
    10681 cttgaacata atcatcttta ttgtctgtat aatttcttgg accgagatag ataactttat
    10741 cattaaaaac gaagttttcc cattttacat cattttccaa caaggcagtt gataggtcat
    10801 tgtcagcatt taaaacagca tcaaacacat tgatatctcc aagttgatag ctatcacgta
    10861 aaaattccat acttgtagca aatgtttttt cactacctgt aagcccatga acgaaaataa
    10921 ccggatatgg aagtttgttt tgcgaataaa ttgcttgact agcacacaaa actatcaaaa
    10981 taaatattaa ataattcttc ataaaatttt cactttttca ccgccaaaaa taataaaatt
    11041 aaaataaaaa atcattactg tcctgtaaaa attatattac atttttaaat catttattct
    11101 taattttatt atattgcgtc tttgttaaat agaataaatg aacaagagtc ctttagaaat
    11161 attagtgtca aaattcgaga aagatggtga attaattaga ataaatgaat ttgtaaatcc
    11221 cgaacttgaa atcacagaat ttgcagacag aatgatgaaa ctcccaaaag gaggcaaagc
    11281 cttatttttt gaaaatacag gtacagattt tccattatta ataaaccatt ttggctccga
    11341 aaaaagaata ttttcaatat tagggataaa aaattgggaa gaaccttcgc aaaaaattga
    11401 cttacttttt aaaaatataa taaatccacc acaaactttt tctgaaaaat tatcggcttt
    11461 atctgaacta aaaaaaataa aaaaatggat gcccaaaaaa gtaaagtcaa aaggaaaatg
    11521 ccaagaagta attgtggaaa atcctgattt gtcgaaactt cctattttaa aatgttggaa
    11581 acatgatggt ggaagattta ttactttgcc tttagtaatt agcgaagatc ccgaaacaaa
    11641 aatcagaaat attggaatgt atagaatgca agtttttgat aataaattaa caggaatgca
    11701 ttggcatttg cataaaggcg gagctgcaca ttttgaaaaa cataaaaaac taaataaaaa
    11761 aatgcctatt tcagttgttt taggcggaga cccaatctta acttattgtg ctagcgctcc
    11821 tcttccacaa gatatagacg aatttatttt ggcaggattt ttaaaaaaca aaaatgtaaa
    11881 attagtaaaa tcaattacca acgatatttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnnna ttagattgcc agactatgga gtagcacata atttagtgtt ggtaaaagca
    12061 aaagcaactt ataaaggaca agcaattaaa attatgaatg ccttgcttgg tgcaggacaa
    12121 atgatgtttt caaaaataat tatttgcttt aacgaaaata ttgatattca agacgataaa
    12181 caagtttttg aaacattatt aaaagtaaat gatttaaaaa acaatacaat tttcagcaaa
    12241 ggtcctgctg atgttttaga ccattcggca tacacatacg catttggcgg caaaatttta
    12301 attgatgcta caaatcctca aaatgaaaaa ttagaaaata gagaaataaa actcattaaa
    12361 aattctgaat ttgataaaac taaaaaactt actggaataa atataattat tgataaacca
    12421 gaagctataa aagataatca cacagctctt tggctaattc taaacaatat agacccaatc
    12481 tatgatgtta aaattgaaga tgattttatt ttaataaatg caagttcaaa aaatttaaaa
    12541 gaaaataatt ttcaaagaaa atggcccaac cctgtgtgtg ctgacaaaaa aactatagaa
    12601 aaagttgata agatatggtg taaatacaca aatatggatt ttgttgaatc gccatcatta
    12661 aaatatattg atttagttag tggagatgcg gcagtttatg aataaaaata ttatttttac
    12721 attttaaaaa attaaaaatt atggaataca atcaagcttt taatgactta attaacaatg
    12781 gttatgaagt aaaaaccaaa caatacctaa acgaaggtct tgagcttttt aaaaagtact
    12841 ctaacggatt ttttggattt tatttaatta tttttggtgt tgctgctatt gcaagtgctc
    12901 ctccactaat gtatgtggga agtgttttaa gtttacttca agctccttta atagcaggat
    12961 tcttccttgt agcaaacgat gtgataagag gcaacactcc aaaatttgcc gactttttta
    13021 atggatttaa cttttttcta cctttagtac ttttaaatat tgtaagttca atatttattg
    13081 ccgttggatt gattttacta attgttccag gaatgtatct tgctgtgtca tactcatttg
    13141 caaatatgtt tattatattt ttcaattatg atttctggac tgcaatggaa ttaagcagaa
    13201 aaattattac aaaaaattgg tggtcatttt tcggattttt acttattctg tttttaataa
    13261 attttgcagg aattttagct tgtggcgttg gtgttttatt cactcttcct ataacttctt
    13321 gcatgttgtt cgttgccttt gaagatattg tgggtagtgc tatgagaaat caacaacaaa
    13381 caaactttta aacttaatat actatgaaga gcataattaa attttcagtc ctaattttgc
    13441 tttcgttatt tatttatttt aatacttatt cgcaagattg tacaactatt ctaacacctt
    13501 ctgtaaaaaa aagtgagact aagctttgtc atactcacta ctgttttgtt tacagcgaaa
    13561 aacacgaaca atcaaaatgg gtagcttata tacttaaaga ttatatgaca gaaagcaatg
    13621 cgaaacgtac agacaaattt atgacagacc ctcttgtcgc aacaggaact gctaacgaca
    13681 atgactacaa aaattcagga tatgacaaag gacatttatt gcctgctgca gacatgagtt
    13741 ttgactcaat agcaatggta gaaactttct acttcagcaa tatgagtcca caagcgccat
    13801 cgttcaatcg tggaatttgg aaaaatttag aaacttatgt tagaacaaca agctcgaaat
    13861 tgggttgtat ttatgttgtt acaggtcctg tgctagaaaa tgacttagaa aatataggcg
    13921 aaaataaagt tagtgttcca aaatattttt ataaaacaat attatatcat tccgacactc
    13981 taacttctgg aatagctttt ctaataccta acgagaaagc tacagataaa gatattttta
    14041 gctatactct ttctattcga gatttagaga aaactactaa gctaaacttt tggcacacac
    14101 ttccaaggtc aatgcaaaac aaagttgaaa agaatacaga tttcgatttt tggaaacaat
    14161 ttgctaaata aattgtttct aaaacaattt atggataaaa cagtattaat agctttttct
    14221 ttaactctaa ttgctggact ttctactggt ataggctcac ttatttcgtt ttttgcaaaa
    14281 aaaactaaca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggaattcctg
    14401 ttgcacttgc tattgccatt cacaatatac ccgaaggaat tgctgtggct gttcctatat
    14461 attatgctac aggttctaaa tccaaagcca tgcggctttc ttttttatcg ggcttagccg
    14521 agccacttgg agcaattata gcttatttaa ttataatgcc ttttataaac gatgttgtat
    14581 taggaattag tttcgcaata accgcaggaa ttatggtttt tatttctttt gatgaattat
    14641 tgccgggtgc tcaaaaatat ggaaatcatc atataccaac ttatggagtt gtaattggaa
    14701 tggcaattat ggcatttagc ctattaattt taaattaatc ttttaaattt acgatttttt
    14761 ctatcacctt gctatgcttg agtaaatcgt caacatactc acgtgtattc gacaaacgcg
    14821 gaactttatg ttgtccacct agttttcctc ttctgcccat ccaagcaaag aagctacctt
    14881 catcaagaac aataatttcg ggcattgcta atgctaagtc gttaaagcgt ttagcatcat
    14941 aatcagaatt tacttctctc aatttcgaat ctaaagcttg aacaaatgtt tgtaaattat
    15001 ttggtttttg ttcaaattca aacaaccatt gatgcgctcc ctctgatgta aaatcttgag
    15061 gatagaatgt tgcagctaca gtaaactctc taacttgtgc tccagttatt tcacaagcat
    15121 ggtttagggc aacctgagca ttatccataa ttacttcttc gccaaaagcg tttacaaaat
    15181 gcttggtgcg accagttatt ttaagtttat atggattttt tgaaacaaaa ctaactgtat
    15241 cgccaataat ataacgccac aaaccagcat tagtagttat taccatagca taattcacac
    15301 cgcattctac accttctaat ggtactgcgt tgtttcttcc tgctaaataa tctgaaagtt
    15361 caataaattc gtaataaatt ccataatcca acattagcat aagtccctca ttatttaaca
    15421 aaggattgct tgatgtttca tcttgaattg caaaaaaccc ttcagacgca ttatatgttt
    15481 ccatataaat catagctgga ttaggaatta aattgcgaaa agtttcttga taaggcgaaa
    15541 aatttactgc tccatgaaca aacaactcta aattgggcca aacctcaaga atattacttt
    15601 taccagtata gctcaatact ttttttatca aaactaaaaa ccaagttggt attccaacta
    15661 aacaggtaac gttcttatta taagttgttt caacaatttt ttctaactta acctcccaat
    15721 cgtctattaa tgcaatatca cttttaggaa ctcggttgaa ataagtccaa aaaggcaagt
    15781 tttttattaa tatagccgac aaatcgccat agtgagaatc tgaatcattt gcatttatat
    15841 ctgttgaacc tcctatagac aatgtttttc caacaaagac cttagactcg ggatacctgt
    15901 cagcataaag cataaaaatg tcttttccac ttctaaaatg acaatcttct aaagcctctt
    15961 tagaaatagg tataaactta cttctatcag aagttgttcc actagatttt gaaaaccact
    16021 tagtttttcc gggccaaaga atattttctt tcccgtgcat agtttcatca atataaggct
    16081 ttaaagaatc ataatcatgc aaaggcattt tattagaaaa atccacacca ttttttactt
    16141 tatctaaatt gtatttcttt ccaatttcag taaaagcacc tttttctaac aattctgcta
    16201 atactttttc ttgcacctca aaaggatgtg aacgaaaata atcgatttct gctaatcgat
    16261 tataatttat taaacttact atagaattta ttaaagacat tttgtcgcga agataaaaaa
    16321 aattattttt ataaaaattt ttgaattatg ttttttattg acattatact tattattatc
    16381 tttatttatt tcatttataa aggagtaaaa aatggattaa taaaagaact tgcaagcctt
    16441 ttggctttaa cactcggcat ttttctagct ataaaatttt atgcaaagtt aatacctatt
    16501 cttgaagata aagcaagttc ttacgcagaa tatttgccaa tatttgcttt tgcaataatt
    16561 tttattgcca cagtagtagt agtttttatg ttttctcatc tattaaatag atttattaaa
    16621 cttataaaac ttcaatggct aaacaaaact ggaggagctg tttttgcaat tctaaaaatt
    16681 agcttaatta ttggtggctt actgtttttt ataaataggc taaacgaaaa agctggtctt
    16741 ttcgaccctg caatatggga caaatcaatt ttatataagt ttattataaa aatttttaac
    16801 ataatttttc catacactaa agaattaaca aacttatttt aaaaatttgg caagtctcaa
    16861 atgaagacat ttcacaaaat caactatatt tgcacacgaa atggataaaa aaagaaacaa
    16921 aatatcaaat tggtttagaa agagttttca acttgtagta tatcatggaa aaacctatga
    16981 tactatcaga acaattaaat tcaatcgatt aggatttaca tttttcattt ttggaatcat
    17041 agtagtttta tttgcaattt tatcagtttt tgtaatttac acaccaataa aacaaatgat
    17101 tcctggctac cctgacaaag aaacaaaaaa gttaatttat gaaaatgcaa ttaaaacaga
    17161 cagtttaatg catgaattag aaatgaaaga ccagtattta aaaatgatac aagaccttgt
    17221 ttttaatgag gtaccaatag atgaagactt tgttgtgcct gttcaaaatt taagcgaaca
    17281 gcaaattatt gatttcaaca atcccacaaa accaagaata aaaatagaag acgatactga
    17341 ttttgttgtt ccagaagaag aatttcctaa tattgttgaa ccgataaaag gaatagtagt
    17401 ttcaaaatat tcgccttctc aaaatcattt tggaattgat atagctgcca ttagtgatga
    17461 agtaataaaa gcaacttacc gcggtacagt aatttcatct gcttatacta tagaaaatgg
    17521 ctatacaata attatccaac acaaatctaa tttaatctcg gtttataaac atgcaaaatc
    17581 aagtttagta aaagaaggag agagagttgc aactggtcaa attattgcta actatggaga
    17641 ttctggcgaa aatagttctg gtcctcattt gcatttcgaa ttgtggaaaa atggcaaatc
    17701 aataaatccc gaaaactata tacaattcta atgaaaaaac aaaaaaatat agcaattttg
    17761 gggtcaacag gctcgattgg aactcaagct ttaaaaatta ttagcgacta tcccgacaaa
    17821 tttcaagctg aagttttagt tgcaaataat aatgttgatt tactaattaa acaagccatt
    17881 gaatttcaac caaatatagt agttattgca aatgaagaac actacgaaaa attaaaaaac
    17941 gccattaatc atttgcctat aaaagttttt gctggagcaa aatctataga gcaagttgtg
    18001 caaatggatt gtgttgacat tgttctaaca gccatggtgg gttttgcagg tcttttgcca
    18061 accataaaag ccatagaagc aaacaaaaca atagctctat caaacaaaga aaccctagta
    18121 gtagcaggac atatcataaa caagctacta gaaaatagta aatcacaaat tattcctgtt
    18181 gattcggaac attctgcaat atttcaatgc ttaattggag aaaatcataa aaatgttgac
    18241 aaattaattt taaccgcttc gggtggtcct tttagaggaa aagatataga ctttcttaaa
    18301 aaagttaaac caaaacaagc acttaaacat ccaaactgga atatgggaag taaagttacc
    18361 attgactctg caagtttgat gaacaaagga ttagaactaa tagaagctca ctggcttttt
    18421 ggaattgatg ccaaaaatat tgaagttgta atacaccccc aatccgtaat acattcttta
    18481 gttgagttcg ttgatggttc attaaaagct cagctaggag taccaaatat gagaattcca
    18541 attcaatatg ctttagattt tccaaatcgc agtgtttcca ataaacatag attttctttc
    18601 aaagattttg aaaatttaac tttcgaacaa ccagatttaa aaacttttag aaatttacaa
    18661 attgctatag attgcctaaa tgcaggtgga acttcgcctt gtgttatgaa cgcagcaaat
    18721 gaagttgctg tagatttatt tttacaagaa aaaatttcat ttgtagaaat ttctgattta
    18781 atagaagaag gtttggcaac aatagaattt attaacaatc caagtttaaa agattgtgtc
    18841 gaaatagaca atataactag aaaaaaaaca tttgaattat ataaaaagat aaaataatgg
    18901 gtggattaat aatggcagga caactattat tgtcattaac acttttagtt gtcattcacg
    18961 agttggggca ttttcttgca gcaagaatgt tcggcatcaa agtaacaaaa ttctttatat
    19021 tctttgacgt tggatttaaa atatggagca aaaaaattgg agatacagaa tttggttttg
    19081 gttggctacc tttaggtggt tatgtgaaaa ttgcaggtat gattgacgaa tcaatggata
    19141 ccgagcaaat gaaacaagag ccaaaagaat gggaatttag gagcaaacct gcttggcaaa
    19201 gactaatcgt aatgttagca ggagttttta tgaatgttat agctggtatt ttaattctaa
    19261 gtatttcaca tatggtttat acaaaggaat atttaccagt aaaaaatgtg gaacaaggaa
    19321 tttacgctta cccttatgct agatacttag gctttgaaac aggagataaa ataattgcaa
    19381 ttgacggaaa accaacacaa agatacgaag atgtattttc aagcaaatta ttttttgcaa
    19441 aatctattac tgttgataga tacggacaaa caaaagaaat tgctattcca gacactcttt
    19501 attcatacct aaaaaatgga ggactcttta taagtcttga taactttaac tttagtattg
    19561 atagctgtgt tgacaacatg ccaatatccg acttgaattt aaaaaagggc agtaaaatct
    19621 tgtcaataaa tcgtgaaagg ataaaatctt ttggacagct aaaagaaatt ttacattata
    19681 acaaaaatag agaacttagt tttgtttttg acaataatgg caaaatagat agtttagata
    19741 tttttattga tagtacagct aagctaggga tatatgctag tttaccaaaa tttacaggtg
    19801 caaaatataa ttttggtact gctcttttat atggctggaa agatgctttc gaaactgtta
    19861 atgcaaatat aaaaggatat ggcttaatat ttgctggaaa agaaaaagtt agagaaagct
    19921 tacaaggacc aataggaatt gctaaggttt atggagcagt ttgggattgg gcaaaattct
    19981 ggagattaac aggtattata tctatgatat tagcttttat aaatgtttta cctatcccag
    20041 cattagacgg cggacatgca ctattccttg ttatagaagt tattacagga cgaaaattgt
    20101 ccgacaagtt tatgggaatt gtgcaagtta ttggaatgtt aattttactt gccttaatgg
    20161 tttttgtaat cgggaacgac atcattaact tattttgagt ctggttgtta gtttaaattt
    20221 ttaatgaaat tatgagaaaa ataatttttg ttttcttagt tttaaattca agtttcttga
    20281 gtctcaatgc tcaggaattt ttagattttt ctaatactgc accttcagaa aaaagaattt
    20341 tgctagttcc tttcgaccca agaatatatg taaacgatgc tacagcaatt atgttgaaaa
    20401 atgaaaggtc aaaccacgac gaattaatgc aatattttag gtatcaattt aatctccaac
    20461 ttcataatgc tatgatggat tcttgtagcg ttgtaagttt attttcagac aataccagaa
    20521 ttgaccaaga agatattaat actttatatt ctttaatttc ttacgaattg gttttagcca
    20581 caaaaaatgc acctgaaaat ccagaagaaa ataaaaagaa atcttttttt gctaagaaaa
    20641 aagaagaaaa agaactacaa cgaagacttg aagaaactaa agaatatgat gcaagaatac
    20701 acaatggcga aattgtaagc aggcgacaaa cgacccaaga catgagctta aatattgtgt
    20761 tccatcaacc cgaagttctt gaagaaatag caaacagacg aaatgttgac ttgtttttat
    20821 ttataaatca attcgagata gttggaaatt atggagaccc ctatttaagt ggaaatacta
    20881 aagctgaaag aaatttaaaa gttcattttt caatttataa cacaaaaggg caaatgatac
    20941 ataatagttt tggaatcact aaattacctt ttaatttaga cgacaaacaa acagttgttg
    21001 aattgtattt tccagaagtt ataagacaaa taattcataa tataagtttt tagaaatgaa
    21061 tacaaacttc atatttgcta caaataatcc aaacaaatta aaagaagtaa gacatattgc
    21121 aaataataaa ttcaatattg ttggaattga cgagtttgga attaccgaag aacttccaga
    21181 aacacaaaat actctagagg ggaatgcact tcaaaaagca agatatttgt atgaaaaaat
    21241 tagtaaaaat tgctttgctg atgacactgg tttagaagtt gaggctctta atggaagacc
    21301 aggaattttc tccgccagat atgcaggaga agagaaatca tctattgata atatcaaaaa
    21361 gttacttaat gaattaaaag attgttcaaa tcgctcagca cagtttagaa ctgttgttgc
    21421 cttaatctta aacgggaaag aatatctttt tgaaggaata ataaaaggca aaataattgg
    21481 tgaattaaaa ggcgaaaatg gctttggata cgatcctgtt tttgtgcctt taggttttga
    21541 aaaaacattt gctgaacttc ctttaaatat aaaaaatacg atttcgcaca gagcacaagc
    21601 ttttgaaaaa ctctttaatt ttttaaaaca ttatgaagta gatttaaaat aaattctttg
    21661 tttttatgtt tataaataaa aaatactata aataataatg aataaaataa attatatact
    21721 aagcattgtt tgcttgatga ttttgtcatt tttctctact gctcaagtcg ctgtaggaca
    21781 atggcgagac cacctacctt ataatcacgg cacaagtttg gctatatctg aaaatgaaat
    21841 ttacatgagt actaatgccg gactaatgcg ttttgataaa agaactagtg aattagaaaa
    21901 actaagcaaa ataaacggat ttagtgatgc gggagtaagt acagtaaaat atagtaaaaa
    21961 attcgacact tttattattg catatactaa tgggaatatt gacctagtac aaaataatac
    22021 aattactaat ataggtgact taaaacgcaa gtttataaat ggagacaaaa ctatttacga
    22081 tattagtcta aatggagatt tcgcatactt agcttgcggt tttgggatta ttgaactaga
    22141 cctaaaacgc aaagaaatta aagaaacttg gtatataggc gaaaatggaa cttatgtgaa
    22201 aattaattct ttagataatg atggtgaata catttatgca gctaccgaca taggaattta
    22261 caaaggcaat tttaacaata aactagttga tttctcaaat tgggaaataa ttacacaaca
    22321 aaatttgcct aacgatttca aatggctcga aggcaaagaa ttttctagtc ttacaatttt
    22381 tgatggaaaa attattgcta attaccacaa tcctacacaa gacgatgcgg acactttagt
    22441 agtttactac aacggaaatt gggaaagact ttaccccgaa gaaaatatgt ttgaagccgt
    22501 tacttctagc tccgaacatt taattttaat aaaccgttat tggataagac ttcttaacaa
    22561 aaattttgaa gaagttaaaa ctgtttggca ttacactata aatgaacaat atcgccaagc
    22621 tattcctgct gatgcgtttt tagaagatga gaaaatttta tggattgctg ataagaaaaa
    22681 tggtttagtt agaacgccag aaccttggga ttataaatca tacattatta atggacctga
    22741 aactgaaaaa gtttatgatt ttgctgtttc agaatcaaaa attataggaa ttaatggtgg
    22801 aatgaccgca gcatggggac caaaatgggc acatggaatg ttctttgaat ttaaagatga
    22861 attttggact tcggctctag ataaacccaa taatggttta gaaggtgtat tcgacttaac
    22921 aacaattact ataaacccta atgacccaaa caacatattt attggctctt gggtaaatgg
    22981 tttgttagaa tatagaaata atgcatatta taaaattcac aatgacgaaa acagttcttt
    23041 gcgacaagct ttaggaaata gcggatatat aagaattgga ggagctgatt ttgatagtga
    23101 aggaaatctt tgggtaacaa attctctagc aactccacaa attcacgttt tagataaaca
    23161 aggaaaatgg actggtataa attatagctc gataattaat gatagaaata ttggtaaaat
    23221 tttaataaca aaagataata taaaatgggt tattgttggt caatctggcg gattgttagt
    23281 ctttgatgac aacggaactc ctgatgtaag aagcgatgac aaagttaaac gacttgacat
    23341 tgtaaacgaa aatggagaac ttattagtaa tgacgtttat tcaatcgccg aagataaaaa
    23401 cggatatatt tgggtaggaa caagaaaagg agttgcagtt tattacaacc ctcaagatgt
    23461 atttacttca aataaattta ctgcaagaca aataaaaatc cctagaaatg acggaactga
    23521 caatgctgat attttattag gaaatgatgt tgttacaaca ataactgttg atggttctaa
    23581 taaaaaatgg tttggaactc aatctggagg agtatattat acaagtgctg atggactaga
    23641 aataatctta cattttaaca cagaaaattc gcctctaatt tcaaataata ttatttgcac
    23701 agaaataatt ccaaatactg gtgaggtgtt ttttggaact gagaaaggaa ttatttcata
    23761 cagaaatatt cctaccgaag gtgatgataa ttttgataaa gtttacacct tcccaaatcc
    23821 tgtgaaacca gattatagag gacctattac aatctctgga ttagttgctg gttcttatgt
    23881 gaagattaca gatatttctg ggaatttggt tttcgaaact cgttccgaag gtggacaagc
    23941 agtgtggtat ggagaagacc ttagaggaaa cagagtgcgt acaggcgtat atttggtttt
    24001 tgctgcaaac gaaacaggct ctaaaaaaaa tgttacaaaa attcttttta taagtggaaa
    24061 tgaataaaaa tgcaaaaaac agaagccata gttcttaata aaattaaata cagcgacagc
    24121 tcttttattg taaatttaat tagcgaagac ttaggtaggt ttgccgctat ggtaagagtt
    24181 tcaaaatctt ctaaatcaaa tataaaacaa gcttcttttt tcccactaaa tattattgaa
    24241 accgaaataa atctcaaaaa taacagagaa gtacaaaccc taacttattg caacaatgta
    24301 gaaaaccttc aaaatatttt actagatata aataagttaa ctatagctca atttattgcg
    24361 gaaattattc taaaaaccat aaaagaaaaa gaaacaaatt caaaaactta taattacatc
    24421 aaaaatttta cactagaatt aaactcaaat tcttccgaaa ccaataacat acatttgctc
    24481 tttttaaaag attttgctgc tctatcagga tttgaaatgt acaataatta ttgtaaagaa
    24541 actcctttct tcaatattaa agaaggatta ttctctcctg tatttacaag cagtacagaa
    24601 tcattagatt tgcacgaaag tcaaatactt ttcaacttat tatcagcaaa aaacactaac
    24661 gaactaataa aacttaattc caacaacaaa agacttttaa tagaaaaaat cttattgttt
    24721 tacgaacacc atattccaaa ttttggcaaa actaaaagtt taaaaatttt acaagaagtt
    24781 tttaatgatt a
//
LOCUS       DAOPNX010000031        24253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_2604_length_24253_cov_7.160013, whole genome shotgun sequence.
ACCESSION   DAOPNX010000031 DAOPNX010000000
VERSION     DAOPNX010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24253
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2604_length_24253_cov_7.160013"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PKN53_04940"
     CDS             complement(<1..348)
                     /locus_tag="PKN53_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88335.1"
                     /translation="MRRLMGHLTDIKSSTQNQVFKYFAKELLKNRKNANEPYSGEEKQ
                     KIESFFVKSKIKMYSFPLKKFSYNANKQDHREKRKQVIEFEKQVIKLFKESGKILMNK
                     NMPSNVNETIQFVE"
     gene            complement(529..927)
                     /locus_tag="PKN53_04945"
     CDS             complement(529..927)
                     /locus_tag="PKN53_04945"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOD88336.1"
                     /translation="MIEHEMKYRVLYADTDAMGVMYYANYLRIYEAARADFMRKLGFS
                     FSEAATKGIVCPAINVNLEYHRFAQFDQEVTVKTCVKEIPRVKFVFHQKMYDKDNNLL
                     NSAIITLGFVNTEKFKAVRCPEWILRYFEG"
     gene            complement(920..1510)
                     /locus_tag="PKN53_04950"
     CDS             complement(920..1510)
                     /locus_tag="PKN53_04950"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOD88337.1"
                     /translation="MIYRPNISNDEIQYLPLSSFEGEIVVVDNIETYNKIKDELFLEN
                     IWGFDTETKPCFKKGMANNTPVSLLQLSSAEKTYLFRLNKLELQKEVINILSSPKYLK
                     VGVSISDDLKALKKLKNFKPQAFFELQNYVKEFGIEEMSLKKLAAIVLNVRVSKAQQL
                     SNWAAEELTEKQIKYAATDSWICREIYLHLKYSQND"
     gene            complement(1500..2030)
                     /locus_tag="PKN53_04955"
     CDS             complement(1500..2030)
                     /locus_tag="PKN53_04955"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HOD88338.1"
                     /translation="MYNTELIYTKETIEFAAVAKEFLSFLESSQASSKDEFIDTSLKI
                     LPLLYLKALQLPKVEDFDDDFSEKFVDEATWSYIQQITSAKLGEDDQYVQVQDATVMN
                     SLDSLNVGLSELYADLYQDIGDFIGAFRLGIDDTMLAALFYCQENFKTYWGIRNLILL
                     KNLHEIKFKTEDDIDL"
     gene            complement(2036..2656)
                     /locus_tag="PKN53_04960"
     CDS             complement(2036..2656)
                     /locus_tag="PKN53_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88339.1"
                     /translation="MKNAIILISLVFITLLASSCSNKESEVVVNGKIITPSENDYQGE
                     KLVVTNKIMYDVQIRNDLIGDRSINNPDWFWENLPSPQDDEFLKDFLNDAIVGKLKTY
                     YYDLSGDYSSFEEIPEAERANYIEKCLNYEFEIVDTTVLPNDVTKMKIQLDHTNIVKL
                     RFLEEWYIAEGVFKKRVIAVAPYFSYLRPDNELITFVFFWVLVDGK"
     gene            complement(2734..2807)
                     /locus_tag="PKN53_04965"
     tRNA            complement(2734..2807)
                     /locus_tag="PKN53_04965"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2771..2773),aa:Arg,seq:tct)
     gene            3043..3996
                     /locus_tag="PKN53_04970"
     CDS             3043..3996
                     /locus_tag="PKN53_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88340.1"
                     /translation="MVKTGICGDIYENDKILDNVLDIPELSLVGFYSPDLHTKEFKEK
                     SKIKSYTQVESLLNDVDAVIALSPLTDSFHLEKLVKNSKHVYFEPSSKCYTEDFNKLN
                     NIIDEANVKVQAGLHHRFNNTFLSAKPFIKKPKFIQSKNFKRFNFDIDSHSVLMDLLI
                     PDIDIVLSLISSNIKNIKTSATSLNYSDPDIINVRLEFMNGSVADLTASRIAAENSHE
                     ISFYCDKNYVQLDLYNNNANLLKKRDPKSSVNLFQENVGDLIVEKINIKPNNSYFDEF
                     SSFAKSIVYDKEPEADVDKILKTYKIIDMIKDKLKLETVRR"
     gene            4099..5769
                     /gene="ettA"
                     /locus_tag="PKN53_04975"
     CDS             4099..5769
                     /gene="ettA"
                     /locus_tag="PKN53_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOD88341.1"
                     /translation="MSDEKIIFSMVKVGKKVPPQRQILKDIYLSFFYGAKIGIIGLNG
                     SGKSTLLKIIAGVDKSYEGEVVFSPGYTVGYLEQEPVLDNNKTVKEIVEEGVHEIVSL
                     LKEYEEVNEKFAEPMSDDEMNKLIERQGELTELLDQHDAWNLDSKLERAMDALRCPDE
                     NEIVAHLSGGERRRVALCRLLLREPDILLLDEPTNHLDAESVHWLEQHLQQYKGTVIA
                     VTHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKTTRLAMEEKQESKRRKTLERE
                     LEWVRMSPKARHAKSKARLSAYDRMLNEDTKQKEEKLEIFIPNGPRLGDKVIVANNIT
                     KAFDDKLLFENFSFSLPPAGIVGIIGPNGAGKTTLFRMIMDQVKPDSGHFEIGETVKI
                     GYVDQTHTEIDPEKTVYQVISGDSEFINVGGRQINARAYVARFNFSGADQEKKCKVLS
                     GGERNRLHLALTLKSESNVLLLDEPTNDIDVNTLRALEEGLENFAGCAVVITHDRWFL
                     DRVATHILAFEGDSQVIFFEGSYSEYEENKKKRLGDRGPQRIRYRKLI"
     gene            5783..9067
                     /locus_tag="PKN53_04980"
     CDS             5783..9067
                     /locus_tag="PKN53_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOD88342.1"
                     /translation="MKKIIIFIFVLSLFSNLSNSQSSYIKQINKGKYSKVEKKLEKSL
                     QKSPNDIELNYAKSYLYNSKKFNKYNSEKAYDYIIKANSNFYYLKDEKARKKLEKILI
                     NTSSITKQIDSVSYNALTDISKNNSIEDYNKFLTYFKLAPQKYKNQAIENRDNLAYDE
                     ALEINTIASYQYFMDNYPISRHFKFAEKNRNKLAFQIASSENTIESYNYFMKTYPYAK
                     EFTEAETNRDELAFNKAKSIDNSSAYENFINNYPNSKQKNEAFQLFETRLFEEITTQN
                     NGDSYISFIENFPNNSFINDAIDSLYLIGSQIKYLPYIKYGLSKSKGNQYTNGIIHYY
                     NLISKDGELSSLKMFSDKFFDYLYLIDTYEDDLEKALFAESIGLTGDLSASHFDNSDE
                     LNQRLIREGAKSGSIQISLMWDNYNDLDLHCIDPFGEEIYYGHRKSYSGGELDVDMNV
                     SPESLRPVENIYWQEGNAPIGQYTVILRHYSNHRCGVNCKDPTKYKIRIKIGNKIKEY
                     TGSISHPQKYRTICTFNYTGTEYGEIELTQENIQKLKNYILESAPNDLSFVALQILIS
                     QNIKNKRWSIALATLKEFENSFKNNSKYNDLVLILKSQLDNSIKINPIYSINSVNGEE
                     YSPVISADNKIIYFCGKERNDNIGGEDIFYSKLIGYNWSKPELDRGLSYRDSNEAPMA
                     LSSDGNTMIQFKNGKIGYCEKTENGWTELEYFPDNINKGSWTGDAMLTSDGNALIFAS
                     ERPGSFNFTAIGLQGYHASNNYASDIYISLREDDTWGEPINIGPSINTIYSERSPFLH
                     PDMKTLYFSSDGHGGLGKYDVFKSTRLSDTCWNCWTEPINLGKEINTEENDWGYTIST
                     DGSKAYFAKYNDKKKNKIYWLNLPTHLRPDMVATVSGKLLDSEKKAVSADIKWEDLST
                     GKNIGQSKSDPVDGSFFIVLPLGKIYGYYVDGNDYFPVSKNIDLRNSKDPVVLEENID
                     IISFKQMIEEEIAVPINNLFFNFAQSSLLEYSLPELRRVAKIIKKNNLSVEISGHTDN
                     IGNDADNQILSEKRADAVKEFLINENCEADKLHTIGYGATKPVATNDTEAGRAKNRRV
                     ELKFIQ"
     gene            9077..10966
                     /locus_tag="PKN53_04985"
     CDS             9077..10966
                     /locus_tag="PKN53_04985"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD88343.1"
                     /translation="MTNKFKILCFLLSITLNYNFSLLAQTNLTSSNSINFDYYLNLAE
                     QYRDSIGNKDLYYANQALNYAEKENDNSKIVKALILIAEISKDNGNLDNSLKCLNKAL
                     PLCKQIDDQDLLHSVYLDLGIIYKNLNQIDLAIEYHNLALELAEQQNDIINQALCLNN
                     IGNIYISQDNFSKALYYYKLTLKLFTNPEINSNLYIATLNNIAVIYMSLGDFENAKIT
                     LLEALALIDEGKYSIKNQFYTNLCSVEFKLKNYSQAEKYAKMSEDILSKSDDKSSRLY
                     LYERIFTLYNEQGIYDKAIDYQNKYIQLKDSIFTDKLNQTLSEYKTKYELDKLEVENL
                     LKNKELENKNKINQILIIVILLITTIFILLINYFIKTKLLNKKLNNLNNLLQTKNKEI
                     KQNLKYSKQIQLSLIANKALPDNVLILDLPKDEVGGDFFIYRNYFDSEICILGDCTGH
                     GSSGALLSIFSINTIDQILNQKLEINEILNQLNNRFLAQISKSEYLKGESLSITIIQR
                     TKDAILYSGSKQKLWKFDNLENEIFEFKTDNIIIGREENKSFSLNKLSYNENDVIFIS
                     SDGYPDQFGDNDKGKLKYQKFREILKIWANSKLDNTDIPENEFLKWKGETEQTDDVLV
                     VAIKI"
     gene            10991..12868
                     /gene="mnmG"
                     /locus_tag="PKN53_04990"
     CDS             10991..12868
                     /gene="mnmG"
                     /locus_tag="PKN53_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOD88344.1"
                     /translation="MTFKYDVIVVGAGHAGCEAAAAAANLGSKVLLITIDMNKIAQMS
                     CNPAMGGVAKGQIVKELDALGGYSGIITDKSMIQYRMLNMSKGPAMWSPRAQCDRMVF
                     STEWRNTLEKIENIDFWQDSVNEILVENYKVIGVKTEMKITFYAKSVILTVGTFLNGL
                     MHIGHTQLKGGRISEPASYGITECLTSMGFTADRMKTGTPMRIDGRTVDFSKLQAQNG
                     DEQIIPFSYLHNYNQTFLKQRPCFVGYTDEEVHDSIRLGLDDSPLYDGTIQSIGPRYC
                     PSIETKLVTFPDKDKHQLFLEPEGETTIEYYLNGFSSSLPWEVQIQSLRKVKGFENVK
                     VFRPGYAIEYDFFHPTQLYNTLETKNIENLYFAGQINGTTGYEEAGAQGMIAAINAHL
                     KINEKPEFTLNRDQAYIGVLIDDLVTKGVDEPYRMFTSRAEFRILLRQDNADIRLTEL
                     SHKLGLATDERFETMLNKVHKRDELLSFYAKQSCKPEKLNPILKERGASELKQAIKLN
                     SVLTRPQIKTNDIIHVFPKLQKLLDSYGERSQEYIDSAEILVKYSGYIEREKLLANKL
                     KRLENIIIPDKINYEDLKSLSTEARQKLTKIKPKTIGQASRISGVSPSDISVLLVFMG
                     R"
     gene            12923..14551
                     /gene="ade"
                     /locus_tag="PKN53_04995"
     CDS             12923..14551
                     /gene="ade"
                     /locus_tag="PKN53_04995"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOD88345.1"
                     /translation="MKKYEGNIVDLFNRKIFKGEVIVENDKIIDIQEKENNSNFYILP
                     GLIDAHVHIESSMLIPQEFAKLAVRNGTVAVVSDPHEIANVLGKEGVEFMIENSKNTP
                     LKVFYSVPSCVPATDFETSGAILNHEDIDELFAKHKLLSLGEMMNYPGVIYEDENVLK
                     KLEVAKKHNAIIDGHIPGISGENLEKYVKAGISTDHECFTIDEAIEKINLGMKILIRE
                     GSAAKNFNELFPIISKFNTMVMLCTDDSHPDDLIKYGHINKILKMGIKQNIDIFDLLI
                     SASVNPVLHYKLPVGLLRKNDFADFIIIDNLKDFNILETIINGQTVYSNNRILFNTKK
                     IKPINKFNAKPISVENLKVENLNKEIKVIEVIDNELITNQIICKPKVENNNLVSDIEK
                     DILKIVVYNRYQENSKPQIGFIKGFGLRDGAVATSVAHDSHNIIAIGNTDQDICNAIN
                     EIMENKGGLTYVKNKDYYTLNLEFAGLMTQDNAENVAQKYETLNKIIKVNGSVLTAPF
                     MTLSFMALLVIPKLKIGDRGLFDVEKFEFTELFV"
     gene            14610..15059
                     /gene="dtd"
                     /locus_tag="PKN53_05000"
     CDS             14610..15059
                     /gene="dtd"
                     /locus_tag="PKN53_05000"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOD88346.1"
                     /translation="MIAVIQRVSRANVKIDSKIHSEINEGLLVLLGITHDDTIEDVKW
                     LNQKIVNLRIFNDENGVMNLSCLDVNGEVLIVSQFTLHAKTKKGNRPSYIEAAKHETA
                     IPLYEEFIKNTEIELKKPVKSGVFGADMKIELVNDGPVTIIIDTKNK"
     gene            15123..16001
                     /gene="deoC"
                     /locus_tag="PKN53_05005"
     CDS             15123..16001
                     /gene="deoC"
                     /locus_tag="PKN53_05005"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOD88347.1"
                     /translation="MENPYIKETPSEKLVNTKVAEVKSKVQDYKRKNILIDILNLIDL
                     TSLNGTDTIGKIKSMTEKVNNFSKEFPKYKNVAAICVYPALVPVVKENLTDKSVKIAA
                     VGAGFPASQTFLSVKAAECDLTVQKGADEIDIVLSLGSFLEKDLEKTAYEVKIIKSAI
                     KEAHLKVILETGELKTIENIFLASLLSMEAGADFIKTSTGKTSISATPEAVYTMCSAI
                     KTYYLKTGRKVGIKPSGGMTVAEDALLYYLIVNEVLGEEWLNNKLFRIGASRLANNLL
                     KDILSFDKKTDFEGYF"
     gene            complement(16003..16980)
                     /locus_tag="PKN53_05010"
     CDS             complement(16003..16980)
                     /locus_tag="PKN53_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOD88348.1"
                     /translation="MKSFKEISKNIKLDLENFEVLLNELFIDEDESLKEFFDFIFKIK
                     GKRVRPALVYLFARMFGETNSTSHDAALLVELIHTATLLHDDVIDEAVLRRGVVSVNK
                     KWDNKTAILLGDYLLAKSMQVTTKHQEYKLFDIITPSVLALSLGELEQQISNFKNFEI
                     NEEKYFKIIKNKTASLLSACCKAGAYSTVKDEIILEKAHEFGENLGIIFQIKDDILDY
                     VGNEKTGKEKGIDIKEGKITLPLIRAFQNMSESNRNQLKVVWQQAFDDAEIRKNIIEL
                     TIKNNGIKSAEQDMQIYRNRALNILNEFPKCSARDALEELIDYVIERDK"
     gene            17201..18337
                     /locus_tag="PKN53_05015"
     CDS             17201..18337
                     /locus_tag="PKN53_05015"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOD88349.1"
                     /translation="MIIVENFQAGHYEQGIEYKYFVPNKINVKWSWTTPTINYLLEKA
                     SIKLGELNSFSRLVPNIDLFIKLHVTKEAVVSSRIEGTQTKIDEALLPENEISPERRN
                     DWKEVNNYIKAIDNAILNLEKLPISSRLIKATHKLLLDSVRGEHKLPGIYRSSQNWIG
                     GKSIADAKYIPPHFTLVDDLMGDLEKFLNNDNIEVPALIKIAIAHYQFETIHPFLDGN
                     GRIGRLLITLFLVEQKILDKPLLYISNYFEKNRELYYDNLHNTRIKNDMLQWIKYFLI
                     GVEQTATLAVDTLSKIISLKTNIEENIRISYGRRSNVALMLLHDLFKNPFVTIDDVTK
                     TCNLSYKAANDLVKKMQEDEYLTEMTGQTRNRIFVFKQYIDIFI"
     gene            18571..19437
                     /locus_tag="PKN53_05020"
     CDS             18571..19437
                     /locus_tag="PKN53_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HOD88350.1"
                     /translation="MTQKQSIQLFEEKKVRTVWDEEQEKWYFSIVDTIAILTDSINPR
                     KYWSVLKTRLKKEGSELATNCSQLKMRSSDNKMYLTDVADTEQLFRLIQSIPSPKAEP
                     FKLWIAQVARERLDQMQDPELSIEQAMTDYKRLGYSDNWINQRLKSIEIRKELTDEWK
                     KRGLQEGVQFASLTDIIYKTWAGKTAKEYKKFKGLKKENLRDNMTNTELVLNMLAELS
                     TTRISETTNPETIEEHSDVARQGGEIAYNARIELESKTGKKVISPLNAKQGIITNENS
                     LKNNENIEQSEE"
     gene            19589..20383
                     /locus_tag="PKN53_05025"
     CDS             19589..20383
                     /locus_tag="PKN53_05025"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOD88351.1"
                     /translation="MLLAFDIGNTNIKVAVFESDKLLQQWRISSDRSRTGDEYFALIN
                     VLFKDYNLDIKRFNNAVISSVVPQLIGAFEIVTKRLLNKEPLIINPDIYHKLPVSIPP
                     TAVYEIGTDLLCDAVQVWETYKQACIVVDFGTALSFVAVGDKANILGITIAPGIGTAF
                     KSLFSNTAQLQSVPLEIPTTSLGINTTMCIQSGMMFGYKGLVEGIINQMKKDMQKQEN
                     INPQNIITIATGGFNNVLTPITTIFDKFDKMLTLNGLNTVTKYALK"
     gene            20396..20743
                     /locus_tag="PKN53_05030"
     CDS             20396..20743
                     /locus_tag="PKN53_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOD88352.1"
                     /translation="MSNEILLYQSNELTERIEVRLENETVWLSQQQMATLFQQTKQNI
                     SLHINNCFREKELDKNSTVKYSLTVQIERVENQNMVSSILEHTTQHSTTKVKMNKNSV
                     QNIINAIAEDLKS"
     gene            20748..21845
                     /gene="corA"
                     /locus_tag="PKN53_05035"
     CDS             20748..21845
                     /gene="corA"
                     /locus_tag="PKN53_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOD88353.1"
                     /translation="MQPKQNKPMNFLNPFHFISVLNRKKNDPKEYIFTGNLKINELDI
                     QLFKYNKEECEEIKNILPKDINKFEKDENNYWLNIYGLSETNKIVSICEKQGIHSLVI
                     QDILDVNQRPKFQEFENFSFLTLKSIVPSENEMITEQISFVFGTNYLISFQERRADFF
                     EHLRVRLRENKGILRESSMDYLLYTMLEAILDNYFKTLTKLDEDTEKFNFTNTKKEPS
                     PNALEIIENNKKLINFIKKSILPIKEFTQLVERAECQSVQKNNLKYFAEIKDLCLTLI
                     DNCDMILSSLESKSNLFFSVQGHRMNQVMKTLTIVSTIFIPLTFIAGIYGMNFSNMPE
                     LKWKYGYFAIWIIIIIVFFTMIMYFRRKKWF"
     gene            21912..22361
                     /gene="queD"
                     /locus_tag="PKN53_05040"
     CDS             21912..22361
                     /gene="queD"
                     /locus_tag="PKN53_05040"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOD88354.1"
                     /translation="MTKIRLTKIFEFEAAHALYNYDGLCRNIHGHSYKLAVTVIGKQN
                     TEPNSPKLGMVMDFGILKNIVKEKIVDKFDHSILLNKKENINSFKNENPMFNRIHVFD
                     FQPTAENMILHFVEIIKKLLPENIKLHSLKLHETASSYAEWYADDQI"
     gene            22365..23102
                     /locus_tag="PKN53_05045"
     CDS             22365..23102
                     /locus_tag="PKN53_05045"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOD88355.1"
                     /translation="MIKSFLFSIIICTTLLSFTKVDDNQDNTPQNSKLNSYSYIDKNY
                     LLGKVKPEEDSIFVKIPKKYCLHRTEYIHKEALKPYLAMYFKAEKQGINLKIVSAFRS
                     FNNQRHIWNRQFHGNSDKITVINSILRYSSMPGTSRHHWGTDIDLLSVHLRYFETEEG
                     KKAYQWLCENAYKFGFYQVYTNKPYIGYNEEKWHWTYLPVSKIYLEEYKKQISYEDII
                     GFNGCEFAEKLNIIENYVFAIDSVLLR"
     gene            complement(23104..23598)
                     /locus_tag="PKN53_05050"
     CDS             complement(23104..23598)
                     /locus_tag="PKN53_05050"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOD88356.1"
                     /translation="MSLSIFDYLSLKGVLSKIKWPNDIYLKNKKLAGILIENTITGNI
                     ITNSIIGIGLNLNQDKFPQDLPNPISLKNVTNIEYNIDDEIQLLSNTIVANFEKYKFK
                     DFNFLRDLYLSNLYKYQEKSRFKSEFQEFEGIIRGVDNYGYLLVEVADKIEKFYFKEI
                     EFLE"
     gene            23600..>24253
                     /locus_tag="PKN53_05055"
     CDS             23600..>24253
                     /locus_tag="PKN53_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOD88357.1"
                     /translation="MEAETFENFLQTKFLGTKRFSLEGGETLIPMLYNLLDLAADEQL
                     ASVIIGMSHRGRLNVLANVLGKPLEMIFREFEGDFDEESYQGSGDVKYHLGYKGKYTS
                     EKNNIIDFHLAANPSHLELVDPVVEGIARAEEDKLLDKNYNKSLPILIHGDSSFAGEG
                     VVMETLNFSELEGYKTGGTIHIIVNNQIGFTTNAEDSRSAYYASDIAKMIQAPILHVN
                     "
BASE COUNT         9470 a         3359 c         3573 g         7851 t
ORIGIN      
        1 ttctacaaat tgtatggtct catttacatt actaggcata tttttattca ttaaaatttt
       61 gccggattct ttaaataatt ttatcacttg tttttcaaat tcaataactt gtttgcgttt
      121 ttcgcgatga tcttgtttgt ttgcattgta agaaaacttt tttaatggga atgaatacat
      181 ttttatttta cttttaacaa aaaaactttc tattttttgt ttttcttcac cagaatatgg
      241 ctcgttagca tttttacgat ttttaagaag ttcttttgca aaatatttaa aaacttgatt
      301 ttgagttgaa gactttatat ctgttaaatg ccccattaat cttctcaaag gagaccttgc
      361 tgtaatatat tgggaatcac ctgtttgacc aatataataa cttagattct ttattatttt
      421 ctttattatt tacctcaatt atataaactg aataagcact ttccaagtgt ttaccttcta
      481 tttctaataa aatataattt tcttttacgt tatttgacat atccccctct acccctcaaa
      541 atacctcaaa atccactctg ggcagcgaac ggctttaaat ttttcggtat ttacaaaacc
      601 taaggttata atagctgagt ttaataaatt attgtcttta tcatacattt tttgatgaaa
      661 tacaaatttt actcttggta tttcttttac gcaagttttt actgtaactt cttggtcgaa
      721 ttgtgcaaat ctatgatatt caagatttac atttattgca ggacaaacaa ttccctttgt
      781 tgcggcttca gaaaaggaaa aaccaagctt tcgcataaaa tctgccctag cagcttcgta
      841 aattcttaaa taatttgcat aatacatcac tcccattgca tctgtatctg catataggac
      901 tctatacttc atttcgtgtt caatcatttt gtgaatattt taagtgtaaa taaatttctc
      961 tacaaatcca cgagtctgtg gctgcatatt ttatttgttt ttcagttagt tcttcggctg
     1021 cccaattact aagttgctga gcttttgata ccctaacatt taaaactatt gctgctaatt
     1081 tttttaaact catctcctct attccaaact ctttcacata attttgtaac tcgaaaaatg
     1141 cttgtggctt aaagtttttc aactttttta atgcttttaa atcatcgctt atagatacac
     1201 caacttttaa gtattttggc gatgataaaa tatttataac ctctttttgt aattctaatt
     1261 tatttaaacg aaaaagataa gttttttcag cactgctcaa ctgtaacaaa gaaactggcg
     1321 tgttgtttgc cattcctttt ttaaaacaag gcttggtttc tgtgtcaaag ccccaaatat
     1381 tttctaaaaa aagctcgtct ttaattttat tgtaagtttc aatattgtca actacaacta
     1441 tttcgccctc gaaagaagac aaaggcaaat actgaatttc atcgttacta atgtttggtc
     1501 tataaatcaa tgtcgtcttc ggttttaaat ttaatttcgt gtaagttttt cagtaatatc
     1561 aagtttctaa ttccccaata tgttttgaaa ttttcttgac aataaaacaa agcagcaagc
     1621 atagtgtcat caattccaag cctaaaagct ccaataaaat ctcctatatc ttgatataaa
     1681 tctgcataaa gttctgataa tccgacattt aaagagtcta aactattcat aactgttgca
     1741 tcttgaactt gaacatattg gtcgtcttcg cctaactttg cagaagtaat ttgctgaata
     1801 taagaccaag tagcttcgtc aacaaatttt tcagaaaaat catcatcaaa atcttcaact
     1861 tttggtaatt gcaaagcttt taaatataat aaaggaagaa tttttaacga agtatcaata
     1921 aactcatctt tagaacttgc ttgcgaagat tctaaaaaag atagaaattc ctttgcaact
     1981 gccgcaaatt ctattgtctc cttagtgtaa attaattctg tattatacat aaaaattatt
     2041 tgccatcaac aagaacccag aaaaacacaa aagtaataag ctcattatca ggacgtaaat
     2101 atgaaaaata aggagcaaca gcaattactc ttttcttaaa aacgccttct gctatatacc
     2161 attcttctaa aaatctaagt tttacaatat tagtgtgatc tagctgaatt ttcattttag
     2221 taacatcgtt tggcaacact gttgtatcaa ctatttcaaa ctcataattc aaacattttt
     2281 ctatataatt tgcccgttcg gcttctggaa tttcttcaaa actgctataa tcaccactaa
     2341 ggtcataata ataagttttt aattttccta caatggcatc attaagaaaa tcttttagaa
     2401 attcgtcatc ttggggactt ggtaaatttt cccaaaacca atcgggatta tttatgcttc
     2461 tatcaccaat caaatcattg cgaatttgca catcatacat gattttattt gtaacaacta
     2521 atttttcacc ttgataatca ttttcagatg gtgttataat ttttccattt acaacaactt
     2581 cgctttcttt gtttgaacaa gacgaagcca aaagtgttat aaaaactaag ctaattaaaa
     2641 ttattgcgtt tttcatactc aaatttttgt caaaattaac aaaaacttta aaaacataaa
     2701 aaaaatctac cgctgtttgc aatagatttt ttgtgatcta gtcaggattc gaacctgaga
     2761 cctacggctt agaaggccgt tgctctatcc agctgagcta ctagaccatt tatttggctt
     2821 gcaaaattat aaaataatat ctattcaaca aaatattttt aaaaatattt tatttacctt
     2881 ataatcaagt agttaaaaca ctcttttaaa cctatttaaa atcatatatc acacaaaaaa
     2941 aatgaaaaat tcctaatttt aacatatatc agttgaaaag tctatctgct tagctgtatt
     3001 ttgcaaaact taaatttata cttttacgta aaatttaata caatggtaaa aactggtata
     3061 tgcggagaca tttatgaaaa tgacaaaatt ttagataatg ttttagatat tcctgaattg
     3121 tctttagttg gtttttatag ccccgacttg catacaaaag aatttaaaga aaaatctaaa
     3181 attaagtcat atacacaagt tgagtcgctt ttaaatgatg ttgatgcagt tatagcatta
     3241 tcacctttaa cagattcttt tcatttagaa aaacttgtaa aaaattctaa acacgtttat
     3301 tttgaaccaa gtagcaaatg ttatacagaa gattttaaca agttaaataa tattatcgac
     3361 gaagccaatg ttaaggttca agctggtttg catcatagat ttaacaacac ttttttatca
     3421 gcaaaaccat ttattaaaaa acctaaattt attcagtcta aaaatttcaa acgttttaat
     3481 tttgatattg attctcactc tgttttaatg gatttgctaa taccagacat tgatatagtt
     3541 ttaagtctca tttcatcaaa tattaaaaat ataaaaacaa gtgcaacaag tttgaattac
     3601 agcgatcccg acattattaa tgttagacta gaatttatga atggaagtgt tgcagactta
     3661 actgcttcaa gaattgcagc cgaaaattct catgaaataa gtttttattg cgacaaaaat
     3721 tatgttcaat tagatttata caataataat gctaatttgc taaaaaaacg agacccaaaa
     3781 tcttctgtaa acctgtttca agaaaatgtt ggagatttaa ttgttgaaaa aattaatata
     3841 aaacctaata attcatattt tgacgaattt tcttcttttg caaaatctat agtttacgac
     3901 aaagaacctg aagccgatgt tgacaaaatt ttaaaaacat acaaaattat agatatgatt
     3961 aaagacaagc taaagcttga aacagttaga agataagata aaacttttat agaaaaataa
     4021 ataatccaaa acaatttttt attcctttag aaatttttta ttaagtttgc ttttcaacaa
     4081 atttaattta tttgacagat gagcgacgaa aaaataattt tttctatggt aaaagtgggg
     4141 aaaaaagttc caccacaaag acaaatttta aaagatattt atttatcatt tttttacggt
     4201 gctaaaattg gaattattgg tttaaatggt tctggaaaat caacactttt gaaaattatt
     4261 gccggtgttg acaaatcgta tgaaggcgaa gttgtgtttt cgcccggata tactgttggt
     4321 tatttggaac aagaaccagt acttgacaat aataaaactg taaaagaaat tgttgaagaa
     4381 ggcgtacacg aaattgtttc tttacttaaa gaatacgaag aagtaaatga aaaatttgcc
     4441 gagccaatgt ctgacgatga aatgaataaa ttaattgaaa gacagggcga attgacagag
     4501 ttgcttgacc aacacgatgc ttggaatttg gatagcaaat tggaaagagc tatggatgct
     4561 ttacgctgtc ctgatgaaaa cgaaattgtt gcacatttat cgggtgggga acgcagacga
     4621 gtagcacttt gcagactttt gcttcgcgaa cctgatattt tacttttaga cgaacccaca
     4681 aaccatttag atgcagaatc tgtacattgg ttagaacaac atttacaaca atacaaagga
     4741 acagtaattg cagttactca cgatagatat ttcttagata atgttgctgg ttggatttta
     4801 gaattggata gaggagaagg aattccatgg aaaggaaatt attcttcgtg gttggagcaa
     4861 aaaactactc gcctagctat ggaagaaaaa caagagtcga aacgccgcaa aacacttgag
     4921 agagaattgg aatgggtaag aatgagtccc aaggcaagac atgcaaaatc taaagctcgt
     4981 ctttcggctt atgatagaat gttgaacgaa gacactaaac aaaaagaaga aaaactcgaa
     5041 attttcattc ctaatggacc aagattagga gataaagtaa ttgttgcaaa caacattact
     5101 aaagcatttg atgacaagct gttatttgaa aattttagct tctcgcttcc accagcagga
     5161 attgttggaa ttattggacc aaacggagca ggaaaaacaa ctctttttag aatgattatg
     5221 gatcaagtaa aaccagattc aggacatttt gaaattggag aaacagtaaa aattggctat
     5281 gttgaccaaa cccacacaga aatagatcct gaaaaaactg tttatcaagt aatttcgggc
     5341 gattcagaat ttataaatgt tggtggaaga caaataaatg ctcgtgctta tgtagcaaga
     5401 tttaatttta gtggtgccga tcaagaaaag aaatgtaaag ttttatcagg aggtgaaaga
     5461 aacagacttc atttagcttt aacattaaaa tctgaatcta atgttttact tttagacgaa
     5521 cctactaacg acatagatgt taatacctta cgtgctttag aagaaggttt agaaaatttt
     5581 gccggttgtg cagtagttat cacccacgac agatggtttt tagatagagt tgctacacat
     5641 attcttgctt ttgaaggaga ttcacaagta atattctttg aaggctctta ttcagaatat
     5701 gaagaaaata aaaagaaaag attaggagat cgaggaccac aaagaattag atataggaaa
     5761 ttaatttaaa aatatataaa ccatgaaaaa aattataata tttatttttg tactttcatt
     5821 attttctaat ttgtcaaata gtcaaagttc ttatataaaa caaataaata aaggaaagta
     5881 ttcaaaagtt gaaaaaaaac ttgaaaaatc tttacaaaag tcgccaaatg atattgagct
     5941 taattatgct aaatcatatt tatataattc aaaaaaattt aataaataca atagcgaaaa
     6001 agcatatgat tatattataa aagcaaattc aaatttttat tacttaaaag atgaaaaagc
     6061 tagaaaaaaa ttagagaaaa tccttattaa cacgtcaagt attacaaagc aaattgatag
     6121 tgtttcgtat aatgctttaa cagacataag taaaaataat agtattgagg attataataa
     6181 gtttttgaca tattttaaat tagctcctca aaaatataaa aatcaagcaa tagaaaatag
     6241 agataattta gcttatgatg aggctttgga aattaacact attgcttctt atcaatattt
     6301 tatggataat tatccaattt cgcgacattt taaatttgca gaaaaaaata gaaataaatt
     6361 ggctttccaa atagctagta gtgaaaacac aatagagtct tataattatt ttatgaaaac
     6421 ttatccttat gctaaggaat ttacagaggc agaaactaat agagatgaat tggcttttaa
     6481 taaggctaaa agcattgata attcatcagc ttatgaaaac tttataaaca attatccaaa
     6541 cagtaaacag aaaaatgaag cttttcaatt atttgaaaca aggctatttg aagaaatcac
     6601 aactcaaaat aacggagata gttatattag ttttatagaa aattttccaa ataatagttt
     6661 tataaatgat gctattgata gcttatattt aattggcagt caaatcaaat atctgccata
     6721 tattaagtat ggtttgagta aaagcaaagg caatcaatat acaaatggaa ttattcatta
     6781 ttataattta atttcaaaag atggagagct ttcaagttta aaaatgtttt ctgataaatt
     6841 ttttgattat ttatatctta ttgatactta tgaagatgat ttagaaaaag ctctgtttgc
     6901 tgaatctatt ggtttaacag gtgatttaag tgctagccat tttgacaatt cagatgaatt
     6961 aaatcagcgt ttaatcagag aaggagccaa atcaggttcc attcaaatct cacttatgtg
     7021 ggataattac aatgatttag atttacattg tattgatcct tttggggaag aaatttacta
     7081 tggtcataga aaatcttata gtgggggcga attagatgta gatatgaatg taagtccaga
     7141 gtcattaaga ccagttgaaa atatatattg gcaagaagga aacgctccta ttggtcaata
     7201 tactgttatt ttaagacatt atagtaacca tagatgtggt gtgaactgta aggacccgac
     7261 aaaatataag ataagaataa aaattggcaa taaaattaaa gaatacacag gaagtattag
     7321 ccatccacaa aagtatagaa caatttgcac ttttaattat actggaactg agtacggtga
     7381 aattgaatta acacaagaaa atattcaaaa acttaaaaat tatattttgg aatctgcacc
     7441 aaatgattta tcctttgttg ctcttcaaat attaatttct caaaatataa aaaataaaag
     7501 atggagtata gcattagcaa cattgaaaga atttgaaaat tcttttaaaa ataatagtaa
     7561 atataatgac ttagttttaa ttttaaaaag tcaactagat aactcaatta agataaatcc
     7621 aatttattca attaattcag taaacggaga agaatattca ccagtaatat cagctgataa
     7681 caaaattatt tatttttgtg gtaaagaaag aaatgacaat ataggtggag aagatatatt
     7741 ttactctaaa cttattggat ataattggtc aaaacccgaa ttagatagag gtttatcata
     7801 tagagattct aatgaagctc caatggcttt aagtagcgat ggaaatacta tgatacaatt
     7861 taaaaatggg aaaataggat attgtgaaaa aacagaaaat ggatggacag aattagaata
     7921 ttttcctgat aatataaata aaggtagttg gacgggcgat gctatgctaa caagcgatgg
     7981 aaatgcactc attttcgctt cagaaagacc aggtagcttt aattttaccg ctataggatt
     8041 acaaggttat catgcgtcaa ataattatgc atctgatata tacataagtc ttcgtgaaga
     8101 cgatacttgg ggagagccaa taaatatagg accatcaata aacacaatat attctgaaag
     8161 aagtcctttt ttacaccctg atatgaaaac cttgtatttt agctcagacg gacatggtgg
     8221 attgggcaag tatgatgttt ttaaatcaac tcgtttatca gatacttgtt ggaattgctg
     8281 gacagagcca ataaacttgg gaaaagaaat taatacagaa gaaaacgact ggggctacac
     8341 tatatctaca gatggtagta aagcttattt tgcaaaatat aatgataaaa agaaaaataa
     8401 aatttattgg cttaatcttc ctactcattt aagaccggat atggtagcaa cagtatcagg
     8461 aaaactttta gactcagaaa aaaaagccgt ttcagcagat ataaaatggg aagatttaag
     8521 tacaggtaaa aatattggac aatcgaaaag cgaccccgtt gatggtagct tttttatagt
     8581 tttaccttta ggaaaaattt acggttatta tgttgatggt aatgactatt tcccggtttc
     8641 aaaaaatata gaccttagaa atagcaagga ccctgtagta ttagaagaaa atattgatat
     8701 aatctctttt aaacaaatga ttgaagaaga aatagccgtt ccaattaaca acttgttttt
     8761 taattttgct caaagtagtt tgttagaata ttcattacca gaattaaggc gagttgcgaa
     8821 gataattaaa aagaataatt taagtgttga aataagtggg catacagata acataggtaa
     8881 tgatgcagat aaccaaattt tatcagaaaa aagagcagat gcagtaaaag aatttttaat
     8941 taacgaaaat tgcgaagcag ataagttaca cactatagga tatggagcaa caaaaccagt
     9001 agcaacaaac gatacagaag ctggaagagc taaaaacaga agagtagaac taaaatttat
     9061 tcaataaatc atacccatga caaataaatt taaaatttta tgttttttac taagtattac
     9121 actaaactat aactttagtt tactagctca aacaaactta acttcaagta atagcataaa
     9181 ttttgattat tatttaaatt tagctgaaca atacagagat tctattggaa ataaagattt
     9241 atattatgca aatcaagctt taaattatgc cgaaaaagaa aatgataatt caaaaatcgt
     9301 aaaagcttta attttaattg cagaaatttc taaagacaat ggaaacttgg ataacagttt
     9361 aaaatgcctt aataaagcat tgcctctttg caaacaaata gatgaccaag atttacttca
     9421 ttctgtatat ttagacttag gcattattta caaaaattta aaccaaatag atttagcaat
     9481 tgaatatcac aatctggcat tagaactggc tgaacaacag aatgatatta ttaaccaagc
     9541 tttatgttta aacaatattg gaaatattta tataagtcaa gataattttt ctaaagcatt
     9601 atattattac aaactaactt taaaattatt cacaaatcct gaaataaact caaatttata
     9661 catagcaact ttaaataata ttgctgttat ttacatgtca ttaggagatt ttgaaaatgc
     9721 aaaaataact ctactcgaag cccttgcact aattgatgaa ggaaaatatt ctataaaaaa
     9781 tcaattttat actaatcttt gttctgtaga gtttaaactt aaaaattact ctcaagcaga
     9841 aaaatatgct aaaatgtctg aagatatttt aagtaaatca gatgataaaa gttcaagatt
     9901 atatctttat gaaagaatat ttactctata taacgaacaa ggaatttatg ataaagctat
     9961 tgattatcaa aataaatata ttcaactgaa agacagcata tttactgata aattaaatca
    10021 aactttaagc gaatataaaa ctaaatatga attagataaa ttagaggttg aaaatttgtt
    10081 aaaaaataaa gaattagaaa ataaaaataa aataaaccaa attttaatta tagtaatact
    10141 tttaataacc actatcttta tattgttaat caattatttc attaaaacaa aattactaaa
    10201 taaaaaacta aataatctaa ataatttatt acaaacaaaa aataaagaaa taaagcaaaa
    10261 tttaaaatat tcaaaacaaa ttcaacttag tttaattgct aataaagctt tgccagacaa
    10321 tgtattaatc ttagatttac caaaagacga agttggtgga gattttttta tatatcgaaa
    10381 ttattttgat agtgaaattt gtattttagg cgactgcaca ggacacggaa gttctggagc
    10441 tttactatcc attttttcaa tcaatacaat agaccaaata ttaaatcaaa aattagaaat
    10501 taatgaaata cttaatcagc taaataatag atttttagct caaatcagta aaagtgaata
    10561 tttaaaagga gaatctttga gtattacaat aattcaaaga accaaagatg caatacttta
    10621 ttctgggtct aaacaaaagt tgtggaaatt tgacaattta gaaaatgaaa tttttgaatt
    10681 taaaactgat aatattatta ttggtcgaga agagaataaa agctttagtt taaataagtt
    10741 aagttacaat gaaaacgatg taattttcat atcaagcgat ggttatccag atcagtttgg
    10801 agataatgat aaagggaaat taaaatatca aaaatttaga gaaatattaa aaatttgggc
    10861 aaattcaaaa cttgataata cagacattcc agaaaacgaa tttttaaaat ggaaaggcga
    10921 aactgaacaa accgatgatg ttttagttgt agcaataaaa atttgattaa ttttgtaaaa
    10981 attttcacaa atgactttta aatatgatgt aatagttgta ggagctggac atgcaggttg
    11041 tgaggcagca gcagcggcag caaatcttgg gagcaaagtt cttcttataa ctattgatat
    11101 gaacaaaatt gctcaaatgt catgtaatcc agcaatggga ggtgttgcta aaggtcaaat
    11161 agttaaagaa cttgatgctt tgggcggata ttctggaatt ataaccgata aatcaatgat
    11221 tcaatataga atgttgaata tgtctaaagg tccagcaatg tggagtccac gcgctcaatg
    11281 tgatagaatg gtatttagca ctgaatggag aaacacatta gaaaaaattg aaaacataga
    11341 tttttggcaa gattctgtaa acgaaatttt ggttgagaat tataaagtta taggtgtaaa
    11401 aacagaaatg aaaataactt tttatgcaaa gtcggttatt cttacagtag gaactttttt
    11461 aaatggctta atgcatattg gacacactca actaaaagga ggtagaattt ctgaaccagc
    11521 ttcttatgga attacagaat gtttaacttc aatgggattt actgccgaca gaatgaaaac
    11581 aggaacacct atgagaatag atggtagaac tgttgatttt tcaaaattac aagctcaaaa
    11641 cggagacgaa caaataattc ctttttcata tttacataat tacaatcaaa cttttttgaa
    11701 acaacgtcct tgcttcgttg gatatacaga tgaagaagta cacgattcta ttcgtttagg
    11761 tttggacgac tcaccacttt atgatggaac catacaaagt attggaccaa gatattgccc
    11821 aagcatagaa accaaacttg taacttttcc tgataaagat aaacatcaat tatttttaga
    11881 accagaagga gagacgacaa tagaatatta tttgaatggt ttttcatcgt cattgccttg
    11941 ggaagtacaa attcaatctt tgcgtaaagt gaaaggattt gaaaatgtaa aagtttttag
    12001 acctggatat gctattgagt acgatttttt ccacccaacc caactttaca atactttaga
    12061 aactaaaaac attgaaaatt tatattttgc cggacaaata aatggaacta caggttatga
    12121 agaagctggt gctcagggca tgatagctgc aataaatgct catttaaaaa ttaatgaaaa
    12181 acctgaattt actttaaacc gcgaccaagc ttatatagga gttttaattg atgatttagt
    12241 tacaaaagga gttgacgaac cttatagaat gtttacctca cgtgcagaat ttagaatttt
    12301 attacgacaa gataatgcag atattaggct tacagaatta tcgcataaac ttggattagc
    12361 cacagacgaa agatttgaaa caatgctgaa caaagttcac aaacgagacg aacttctttc
    12421 attctatgca aaacaaagtt gtaagcccga aaaattaaat ccaattttaa aagaacgtgg
    12481 tgcttcggaa ttaaagcaag caataaaact aaattccgtt ttgactcgtc cacaaataaa
    12541 aacaaatgat attatccacg tatttccaaa attacaaaaa ctattagact cttatggaga
    12601 aagatctcaa gaatatattg actcagcaga aattttagtg aaatattctg gttacataga
    12661 aagagaaaaa ttattagcaa acaaactaaa aagattagaa aacataatta tacctgacaa
    12721 aattaattac gaagatttaa aatctctttc tactgaggca agacaaaaat taacgaaaat
    12781 taaacccaaa accataggac aagcatcaag aatttcagga gtttcacctt cagacattag
    12841 tgttttgtta gtttttatgg gtagataaaa aatgttccac gtggaacaat taaagtaaaa
    12901 cttgaaatta aaatgtttta ttatgaaaaa atatgaagga aatattgttg atttattcaa
    12961 cagaaaaatt tttaagggag aagtaattgt agaaaacgac aaaattattg atattcaaga
    13021 aaaagaaaat aatagtaatt tttacattct cccaggctta atagatgcac atgtacatat
    13081 agaaagttca atgttaattc cgcaagagtt tgcaaaattg gcagttagga acggtacagt
    13141 agcagtagtt tcagatccac atgaaattgc aaatgttttg ggaaaagaag gagtggagtt
    13201 tatgatagaa aattcaaaaa atactcctct taaagttttt tactctgtac catcatgtgt
    13261 accagccaca gattttgaaa cttctggagc aatattaaac catgaagata ttgacgaact
    13321 atttgctaaa cataaacttt taagtcttgg cgaaatgatg aattacccag gagttatata
    13381 tgaagatgaa aatgttctaa aaaaattaga agttgcaaaa aaacataatg caataattga
    13441 cggtcatata ccaggcattt ctggagaaaa tttagaaaaa tatgtcaaag caggaattag
    13501 taccgaccac gaatgtttta caattgatga agcaatagaa aaaataaatc ttggaatgaa
    13561 aattttaatt cgcgaaggtt ctgccgctaa gaattttaat gaactttttc caataatttc
    13621 caaattcaat actatggtca tgctttgcac agacgactca cacccagacg acctaattaa
    13681 atatgggcat ataaacaaaa tattgaaaat gggaataaaa cagaatattg acatttttga
    13741 cctgcttatt tcagcttctg ttaatcctgt tttgcattat aaattacctg ttggactttt
    13801 acgcaaaaat gattttgcag attttattat aattgacaac ctaaaagatt ttaatatttt
    13861 agaaacaatt attaatggtc aaactgttta ctcaaataat agaattttat ttaacactaa
    13921 aaaaattaaa cctattaata aatttaatgc aaaaccaatt tctgtagaaa atcttaaagt
    13981 tgagaattta aacaaagaaa taaaagttat tgaagttata gataatgaac taatcactaa
    14041 ccaaattata tgtaaaccaa aagttgaaaa caataattta gtttcagata ttgaaaaaga
    14101 tattttaaaa atagttgttt ataatagata tcaagaaaat tcaaaaccac aaataggctt
    14161 tatcaaaggt tttggtttac gcgatggtgc agtagctact tctgttgcac atgacagtca
    14221 taatatcatt gctataggta acacagacca agatatttgt aatgcaatta acgaaattat
    14281 ggaaaacaaa ggtggtttaa cctatgttaa aaataaagac tattatactt taaacttaga
    14341 gtttgcagga ctgatgactc aagacaatgc ggaaaacgta gcacaaaaat atgaaacttt
    14401 aaacaaaata ataaaagtaa atggctctgt attaacagct ccatttatga ccttatcgtt
    14461 tatggcttta ttggtaatac caaaactaaa aataggagat aggggattat ttgacgtgga
    14521 aaaatttgaa tttacagaac tttttgtctg aatgttccac gtggaacaaa ttataactat
    14581 ctatttttca ataatttaaa acaaataata tgatagcagt aattcaaaga gtgtcgagag
    14641 ctaatgtgaa aattgattca aaaattcatt cagaaatcaa tgaaggctta ttagtcttgt
    14701 taggaatcac acatgacgat acaatcgaag acgttaaatg gcttaatcaa aaaattgtaa
    14761 atctaagaat ttttaatgac gaaaatggag ttatgaatct aagttgctta gacgttaatg
    14821 gagaagtatt aattgtaagt cagtttacat tgcatgctaa aacaaaaaag ggaaatcgcc
    14881 catcttatat tgaagcagca aaacacgaaa cagcaattcc tctttatgaa gaatttataa
    14941 aaaacacaga aattgaattg aaaaaacccg taaagtctgg tgttttcggt gctgatatga
    15001 aaattgaact agtaaatgat ggtccagtta caatcattat agacactaaa aacaaataat
    15061 taaatagaaa ttattatatt tgcaaaagat aaagctaaac aaaaatttaa aattaaaata
    15121 atatggaaaa tccttacatc aaagaaacac cttcagaaaa acttgtaaac acgaaagttg
    15181 ctgaagttaa atctaaagtt caagattata aaagaaaaaa tattttaatt gacattttaa
    15241 atttaataga tttaacaagt ttaaatggaa cagatactat cggcaaaatt aagtcgatga
    15301 cagaaaaggt aaataatttt tcaaaagaat ttcctaaata taaaaatgtt gctgcaattt
    15361 gtgtctatcc agctttagtt cctgttgtaa aagaaaattt aacagataaa tctgtaaaaa
    15421 tcgcagcagt aggagcaggt tttccagcat ctcaaacatt tttatcggtt aaagctgctg
    15481 aatgcgacct aacagtacaa aaaggagctg acgaaattga tatagtttta tctttgggta
    15541 gctttttaga aaaagactta gaaaaaactg catacgaagt gaaaattata aaatctgcaa
    15601 ttaaagaagc acaccttaaa gtaattttag aaactggaga acttaaaaca atagaaaata
    15661 tatttttagc ctctttactt tctatggaag ctggtgctga ttttataaaa acatcaactg
    15721 gaaaaacatc aatatcagca acacccgaag ctgtttacac aatgtgttcg gcaataaaaa
    15781 catattatct aaaaactggt agaaaagtag gaataaaacc aagtggagga atgacagttg
    15841 cagaagatgc tcttttatac tatttaattg taaatgaagt acttggagaa gaatggttaa
    15901 ataataaatt atttagaata ggtgcttcaa gattagcgaa taatttatta aaagatattt
    15961 tgagctttga caaaaaaact gattttgaag gatattttta aattatttat ctctctcaat
    16021 cacatagtca attaattctt ctaaagcgtc tcgtgctgag catttaggga attcattcaa
    16081 aatatttaat gctctgtttc tataaatctg catatcttgc tctgcagatt ttattccatt
    16141 gtttttaatt gttaattcta taatattttt tcgtatttct gcatcatcaa aagcctgttg
    16201 ccacacaact tttagttgat ttctattaga ttcagacata ttttgaaaag ctcgaattag
    16261 tggcaaagta attttacctt ctttaatatc aattcctttt tcttttccag ttttttcatt
    16321 ccctacataa tcaagaatat catctttaat ttgaaaaatt atacctaaat tttcaccaaa
    16381 ttcatgagct ttttctaata ttatttcatc tttcacagtt gaataagctc ctgctttaca
    16441 acaagcagag agtaatgacg cagttttatt ttttattatt ttaaaatatt tttcttcatt
    16501 aatttcaaaa tttttaaaat tagaaatctg ttgttctaat tcgcctaaac tcagtgctaa
    16561 aacagatgga gtaattatat caaataactt atattcttga tgtttagttg ttacttgcat
    16621 tgattttgcc aaaagataat cgccaagtaa aattgcagtt ttattatccc attttttatt
    16681 tacacttaca acacctctac gtaaaactgc ctcatcaata acatcatcgt gaagtaaagt
    16741 agcagtatgt atcaattcta ccaaaagggc tgcatcatga ctggtagaat tagtttctcc
    16801 aaacattctt gcaaataaat aaactaaagc tggtctaact cttttacctt taattttaaa
    16861 aataaaatcg aagaattcct ttaaactctc atcttcatca ataaaaagct cattcaataa
    16921 aacctcaaaa ttttcaaggt ctaacttaat attttttgat atttctttaa aactcttcac
    16981 tatataattt cagactacaa atatatcttt ttttacttat gtttaaataa aaaattttct
    17041 ttcacaagaa taaaggcaaa aaaattgaaa agaaagtaaa ataattgttt ttttatttta
    17101 cttttgtgct ttaaagtaaa ataagaattt tatattttac ttttgcgtct gaaagtaaaa
    17161 taagaatttt tttattttag ttaaataaca ttaaataatt atgataattg tagaaaattt
    17221 tcaagctggt cattacgaac aaggtataga atataaatac tttgtaccaa ataagataaa
    17281 tgtcaaatgg agttggacca caccaacaat aaattatctt ttggagaaag cttctattaa
    17341 attaggagaa ttaaactctt tttcacgatt agtgccaaat atcgacttgt ttataaaatt
    17401 gcatgttact aaagaggctg ttgtatcaag cagaatagag ggtacacaaa caaagattga
    17461 cgaagctcta ttaccagaaa acgaaattag tcctgaaaga cgcaatgatt ggaaagaagt
    17521 aaataattat atcaaagcta tcgataatgc cattcttaat ttagaaaaac tacctatttc
    17581 atctcgactt attaaagcta ctcacaagtt actattagat agtgttagag gagaacataa
    17641 attgccaggt atttaccgtt caagccaaaa ttggattgga ggtaaatcaa tagctgatgc
    17701 gaaatatatt ccaccacact tcacacttgt agatgattta atgggagatt tagaaaaatt
    17761 tcttaataat gacaatatag aggttcctgc tttgataaaa atagctatag ctcattatca
    17821 attcgaaact attcaccctt tccttgatgg taatggaaga ataggaagat tgcttataac
    17881 actatttctt gttgaacaaa agattttaga caaaccatta ttgtatattt caaactattt
    17941 tgaaaaaaac agagaattat attacgacaa tctacataat acaagaataa aaaacgatat
    18001 gttgcagtgg attaaatatt ttcttatagg tgttgaacaa accgcaactc ttgctgttga
    18061 cactttatct aaaatcatta gtttaaagac aaatattgaa gaaaatatac ggatttctta
    18121 cggaagaaga agtaatgtag ctcttatgtt attacacgat ttatttaaaa atccttttgt
    18181 tacaattgac gatgttacta aaacatgtaa cttatcctac aaagcggcaa atgacttagt
    18241 aaaaaaaatg caagaagatg aatatcttac agaaatgaca ggccaaacca gaaatcgaat
    18301 atttgttttt aaacagtata ttgacatttt tatttaaaaa tggtattaaa tcgtataaat
    18361 aaacaaaaga acatttttat attttacttt tacgtctgaa agtaaaataa aattttcttt
    18421 attttacctt cgctaatttt cctacaatat attttccaaa taaaaatttt atcttttata
    18481 ggaataaagg caaaaaaaat gtaaattagc attttaacaa aaatgaaata aggtgtacta
    18541 taataattaa gatcaaaaaa tacaaacaac atgacacaaa agcaatccat acaattattt
    18601 gaagaaaaaa aagtccgtac tgtttgggac gaagaacaag aaaaatggta tttttcaata
    18661 gtagatacca ttgcaatatt aacggatagt ataaatccaa gaaaatattg gagtgtttta
    18721 aaaactcgat taaaaaagga aggcagcgag ttggctacaa attgtagtca actgaaaatg
    18781 cgctcgtcag acaacaagat gtatcttacc gatgtagctg acacagagca acttttccgc
    18841 ttgattcaat ctataccttc accaaaagca gaaccattca aattgtggat tgcacaagta
    18901 gcacgtgagc gtttagatca aatgcaagat ccagaactat caatagaaca agcaatgaca
    18961 gattataaac gacttggcta ttccgacaac tggattaacc aacgtttaaa aagcattgaa
    19021 atacgcaaag aacttacaga tgaatggaaa aagcgtggat tacaagaagg tgttcaattt
    19081 gcttctctta ctgatatcat ctacaaaaca tgggcgggaa aaacagcaaa ggaatataaa
    19141 aaatttaaag gtttaaaaaa agaaaattta agagacaaca tgaccaatac agagttagta
    19201 ttaaatatgc tggcagaatt gtccactaca cgaatttctg aaactacaaa ccctgaaact
    19261 atagaagagc acagcgatgt agcacgtcaa ggaggagaaa tagcctacaa cgcaagaatt
    19321 gaattggaat caaaaacagg aaaaaaagta atttctccgt taaatgccaa acaaggtatt
    19381 attacgaacg aaaatagtct aaaaaacaac gaaaacatag aacaaagcga agaataacaa
    19441 tactaactgg ttattcggaa attccgaaca accactcaac atggtgcaat atcaagtaaa
    19501 agcaaataaa atttacattt cttctctttc ttaacaatct aaattcaaaa caatttatta
    19561 cttttgaaat aaaaataaac aataaaatat gttattagca tttgacattg gcaacacaaa
    19621 tattaaagtt gcagtatttg aatcagataa gttactacaa caatggcgta tcagcagtga
    19681 tagaagtcgt actggagacg aatattttgc acttataaat gttctattta aagattataa
    19741 tttggatatt aaacgtttta acaatgctgt aataagttct gtagtgccac aacttatagg
    19801 agcttttgag attgtaacaa aacgtttgtt aaacaaagaa ccgcttataa taaacccaga
    19861 tatttatcac aaactgcctg taagcatacc acctacagcc gtgtatgaaa taggtacaga
    19921 tttgctttgc gatgcagtac aagtttggga aacctacaaa caagcttgta ttgttgtaga
    19981 ttttggaaca gctctatctt ttgttgctgt tggcgacaaa gctaatattt taggtattac
    20041 catagctcca ggcattggta cagcttttaa atctctgttt agcaatactg cacaacttca
    20101 gtcagtaccg ttagaaattc caacaacaag tttgggcatt aataccacaa tgtgcatcca
    20161 aagcggaatg atgtttggct ataaaggatt agtcgaaggc ataataaatc agatgaaaaa
    20221 agatatgcaa aaacaagaaa atatcaatcc acaaaatatc attactatag ctacaggcgg
    20281 tttcaataat gtactaacac ctattacaac aatatttgat aaatttgata aaatgctcac
    20341 cctcaatgga ttaaatacgg ttacaaaata tgcattgaaa taaataagta taaaaatgag
    20401 taatgaaatt ttattatatc aatcaaatga actaactgaa cgtattgaag taaggttaga
    20461 aaatgaaact gtttggctat cacaacagca aatggcaact ttgtttcaac aaacaaagca
    20521 aaacattagt ttacatatca acaattgctt tcgagagaaa gaattagata aaaattcaac
    20581 tgtcaagtat tccttgacag ttcaaattga aagagtagaa aatcaaaata tggttagttc
    20641 cattttagaa cataccactc aacacagtac tacaaaagtg aaaatgaata aaaactctgt
    20701 gcaaaatatt attaatgcaa ttgctgaaga tctaaaatca taaaactatg caaccaaaac
    20761 aaaataaacc aatgaacttt ttaaatcctt ttcatttcat atcagttctt aatagaaaaa
    20821 agaatgaccc aaaagaatat atttttacgg gaaatttaaa aattaatgaa ttagatattc
    20881 agctttttaa gtataacaag gaagaatgtg aagaaattaa aaacatctta ccaaaagaca
    20941 taaataaatt tgaaaaagat gaaaataatt actggctaaa tatttatgga ttaagcgaaa
    21001 caaacaaaat agtttcaatt tgtgaaaaac aaggcataca tagcttagta attcaagata
    21061 ttcttgatgt taaccaaaga cctaaatttc aagaatttga aaatttttca tttcttacat
    21121 taaaatctat tgttccttct gaaaatgaga tgattacaga acaaataagt tttgtttttg
    21181 gcacaaatta tttaatctct tttcaagaaa gaagagcaga cttttttgaa cacctaagag
    21241 ttagactaag agaaaataaa ggtattttaa gagaatcaag tatggattac ctgctttata
    21301 caatgcttga agctattctt gataattatt ttaaaactct tacaaaatta gatgaagaca
    21361 ctgaaaaatt taatttcacc aacaccaaaa aagaaccttc accaaatgca ctcgaaatta
    21421 tagaaaataa taaaaagctg attaatttta ttaaaaaatc tattctgcca ataaaagaat
    21481 ttactcaatt ggtagaacgt gctgaatgtc aatctgttca aaaaaataat ttaaaatatt
    21541 ttgccgaaat aaaagattta tgcctaaccc ttatcgataa ttgcgatatg attctttcat
    21601 cattagaaag taaatcaaat ttgttttttt cagtgcaagg acatagaatg aaccaagtaa
    21661 tgaaaacact tacaatagtt tcaactatat ttattccttt aacttttatt gcaggcattt
    21721 atggaatgaa tttttcaaat atgccagaac ttaaatggaa atatggctac tttgcaatat
    21781 ggataattat tattatcgtt ttctttacaa tgattatgta ttttcgtaga aaaaaatggt
    21841 tttaacaaat tcattcccaa attactctct caaaacattt ttttactatt tttgttaaat
    21901 aaaataaaga aatgacaaaa attagattaa caaaaatatt tgaattcgaa gcagcacacg
    21961 ctttgtacaa ttacgatggc ttatgtcgta atattcacgg acattcgtac aaacttgctg
    22021 ttacagttat tggaaaacaa aatacagaac ctaactcgcc taaactcggt atggttatgg
    22081 actttggcat attaaaaaat attgtcaaag aaaaaatagt tgacaaattc gaccatagta
    22141 ttttattaaa taaaaaagaa aatataaatt catttaaaaa tgaaaatcca atgtttaata
    22201 gaatacatgt atttgatttt caacctactg ccgaaaatat gattttacat tttgttgaaa
    22261 ttattaaaaa attattacca gaaaatataa aacttcatag cttaaaactt cacgaaacag
    22321 caagttcata cgcagaatgg tatgctgatg accaaattta aaaaatgatt aaatcttttt
    22381 tattttcaat aataatatgt actactcttt tatcatttac taaagttgat gataatcaag
    22441 ataatacacc tcaaaacagt aaattaaact catattcgta tattgataaa aattatcttc
    22501 ttggtaaagt aaaacctgaa gaagacagca tttttgtaaa aattccaaaa aaatattgtt
    22561 tacatagaac tgaatacatt cataaagaag ctcttaaacc ttatcttgca atgtatttta
    22621 aagcagaaaa acaaggaatt aacctaaaaa tagtttctgc cttcagaagt ttcaataacc
    22681 aaaggcatat ttggaataga caatttcatg gaaattcaga taaaattaca gtaattaatt
    22741 caatacttag atattcttca atgccaggaa cttcgcgaca ccactgggga acagatatag
    22801 atttattaag tgtacattta agatattttg aaacagaaga aggcaaaaaa gcttaccaat
    22861 ggttatgtga aaatgcttac aaatttggat tttatcaagt ttataccaat aaaccatata
    22921 taggatacaa cgaagaaaaa tggcattgga catatttacc tgtttctaaa atttatttag
    22981 aagaatataa aaaacaaatt tcttacgaag atattattgg atttaatgga tgtgaatttg
    23041 cagagaaact aaatattatc gaaaattatg tatttgctat tgatagtgta ttattacgat
    23101 aatttattcc aaaaattcta tttctttaaa ataaaacttt tcaattttat cagctacttc
    23161 aacaagtaaa taaccataat tatcaacacc acgaattatt ccctcaaact cttgaaattc
    23221 tgatttgaat cttgattttt cttgatattt atacaaatta ctcaaatata aatctctaag
    23281 aaaattaaaa tctttaaact tatatttttc aaaatttgct acaattgtat ttgataataa
    23341 ttgaatttca tcatcaatat tatattcgat atttgttaca ttttttaaag aaataggatt
    23401 tggtaaatct tgtggaaatt tatcttgatt caaatttaaa cctattccaa ttatcgagtt
    23461 ggtaataata ttgcctgtaa ttgtattttc gataagtatt ccagcaagtt ttttgttttt
    23521 tagataaata tcattgggcc attttatttt gcttaaaaca ccctttaaac tcaaataatc
    23581 aaaaatactt aaagaaataa ttgaagcaga aacttttgaa aattttctgc agacaaaatt
    23641 tctgggtact aaacggtttt cattggaagg aggggaaact cttattccaa tgctttataa
    23701 cttattagat cttgcagcag atgaacaatt agcgtcagta attattggaa tgtcgcatcg
    23761 aggaagactg aatgtacttg caaatgtgct ggggaaacct ttggaaatga tttttcgaga
    23821 atttgaaggg gattttgatg aagaatctta tcaaggttca ggggatgtta aatatcatct
    23881 tggttataaa ggaaaatata catcagaaaa aaataatata atcgattttc atcttgcagc
    23941 aaatccaagt catttggaac ttgtcgaccc agttgtagaa ggaattgcaa gagcagaaga
    24001 agataaatta ttagataaaa attataataa atctttaccg attttgattc acggggattc
    24061 ttcatttgcg ggagagggtg ttgtgatgga aactttgaac ttctcggaac ttgaaggata
    24121 taaaacaggt ggaacaattc atattattgt aaataatcaa ataggattca caaccaatgc
    24181 cgaggattct cgttcagcat attatgcaag cgatattgca aaaatgatcc aggccccaat
    24241 tttgcacgtc aat
//
LOCUS       DAOPNX010000032        23623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_2699_length_23623_cov_6.805796, whole genome shotgun sequence.
ACCESSION   DAOPNX010000032 DAOPNX010000000
VERSION     DAOPNX010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23623
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2699_length_23623_cov_6.805796"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..861
                     /locus_tag="PKN53_05060"
     CDS             100..861
                     /locus_tag="PKN53_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88358.1"
                     /translation="MKKIFIFLILISTFGYLVAQQKIDKAFPYLTWVDPQSGEKSSQG
                     ELFGYDVLVFTPVHQRIENLPKSDPCYKSENNLDFYLVGRYKNESMTDAVNILYSPGP
                     SWDPNFVITDKNNKVIWNFICENMCINLTGVIYTSGNVDKMFDERCKFQLQNNRVVEA
                     KQPYLYVGIKDKLLKPVKLYSDKTGGEVIATLPVGYEVEVLLSENYDKFAENIDYPIN
                     YLVRTSFGLVGWLRLTSEDTYHLDPVIRGLGYLGD"
     gene            875..1456
                     /locus_tag="PKN53_05065"
     CDS             875..1456
                     /locus_tag="PKN53_05065"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HOD88359.1"
                     /translation="MKKYILYLVLILACVNVNAQSFKLPEKMINSEDSLEPLTEEEIS
                     EIQGKVDEFYHKMQKQYSDYDEMAKQYYVEFRTDTFAIEIIENLRSSKRIPELEYSII
                     VSDVNAAYDVLLNKYYKLLRANLNEEKQEMLKQSQLNWLKFKTSELKLCGELFLEDGS
                     IGEIKARYYDTELIKSRTIRLFEYLVEISAYVD"
     gene            complement(1616..2917)
                     /locus_tag="PKN53_05070"
     CDS             complement(1616..2917)
                     /locus_tag="PKN53_05070"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOD88360.1"
                     /translation="MAKQTDTKTARQKYIEISYKNAGKYKYNAGDSVAVIFSGGPASG
                     ANSVISSLALNFINAGIPLYGILKGFENIENFGTDELKTLEEGEHYIVFTREVAGIRG
                     NAGVVLKTSRANPGKDIKKVEDIEDPKKNSKLNNIINAFESLNVKVAITIGGDDTLKL
                     ANFLHLMGFPTIHVPKTIDNDYFGIPWTFGYWSAVETAKDALINFREDARSTDSYFII
                     ELMGRKTGWITYAAGVSAQATMMLAVEDFLDQKYIDINELAKKIVDQIIAREKEKRNY
                     GVICVAEGLVDKLPDDKVAKEKDKHGNIYYGLTEVCKLIADAAKKEYFKRTGKNKKII
                     PRQIGYETRCVRSSAFDTTLGSMLGYGAYKLFEENKFGNMVSVEDNFDIKPIPFSELI
                     DPETLLTKIRTVNPNKDFYKLKESLSYHVGQKNFENGNDEY"
     gene            3135..4115
                     /gene="pfkA"
                     /locus_tag="PKN53_05075"
     CDS             3135..4115
                     /gene="pfkA"
                     /locus_tag="PKN53_05075"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOD88361.1"
                     /translation="MAVLKKIAVLTSGGDAPGMNAAIRAVVRRAIKEDIKVVGVMRGY
                     DGLVKGDFVPLLSKSVSSIIQLGGTILKTARSKEFMTPEGRQIAYNNLVENEIDALVV
                     IGGDGTFKGARIFTEEFNFPTVGIPGTIDNDLFGTDMTIGYDTALNTVVEAVDKIRDT
                     AASHERVFLIEVMGRDAGFIALRAGIACGAEDVLIPETPSDLEGLHEKLTRYIDTKKT
                     SSVILVAEGDEEGGAFEIKEKMSKFKGDYELRVTVLGHIQRGGSPSAYDRYLASRLGI
                     GAVEALIDDQRSIMIGMINREVVHVPFNKTTKHHKTIDQKMVDIVEILNA"
     gene            4122..6572
                     /gene="priA"
                     /locus_tag="PKN53_05080"
     CDS             4122..6572
                     /gene="priA"
                     /locus_tag="PKN53_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOD88362.1"
                     /translation="MQKPVYVEVLLPLALENTLTYVVPKEINNTPKIGSRVIVELGKR
                     KIYTGIITKIHSEKPDYECKEILSVKDDFPILSEKQTELLNWISDYYCCTQGETLAAA
                     MPSKLLASSETQVWFNKNCESDYSPTQTEINILNYLENSPKASVSDIAKAVNLKNPLK
                     HIENLEEQGFLNLSQQIVNAYKPKYQNFLSFNLDLAQEKLDEIEKILTKNNKQKEVLQ
                     FFNEKCLQENITSLEYSEKLLMQELNVAKSTIQTLVNKGLIEIVKKEETRFETINVES
                     AKSLKLSEAQTEVYNQIKNNFGNSKPVLLHGVTSSGKTEIYIKLIKEVLKTGKQILYL
                     LPEIALTTQMINRLRNYFGDKIGVFHSKYTVDARSEIYLKLLKKELQIVLGVRSAVLL
                     PFSDLGLIIVDEEHETSFKQQDPDPRYSARDTAVVLSKLHKAEIVLGSATPSIESYNN
                     AINSKYLLTTLNQRYGNVEMPEIVLADMSDAYKRKINSGHFHPILINEIKKVLEKNQQ
                     VILFQNRRGYTSIIECRDCGWVPHCKSCNVSLTYHKFRNKLVCHYCGEMYDIVNNCGN
                     CNSEKLSHKGFGTEKLEDEVSKFFPHARIARLDYDTASTRNKFSRIIEGFEKHEFDIL
                     VGTQMVTKGLDFQKLALVGVLNADNLLNYPEFRAYERAFQLLTQVSGRAGRRDKRGKV
                     IIQTYDVLNPIIQYVKNEDYLSMFNSQLAERQLFNYPPFSNYIIIKLKHKDRAKVHRT
                     AHVFAMMLRTKLGNRVRGPEEPLINKISNYFILNVHIRFEKTISGTQLRKFIKNRIAK
                     IKEESDFSSVRFEIDVDP"
     gene            6679..7014
                     /locus_tag="PKN53_05085"
     CDS             6679..7014
                     /locus_tag="PKN53_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88363.1"
                     /translation="MKKIRIILNFALEPWAPPNITTPFPSDMLIDYVKVYDVKKDCSN
                     NLNVCDYNFGVHDNKVKKSITIGNGSCINSLSVGQNVFLRASEGVLINGDFTVPDGAQ
                     LYIDVNTCY"
     gene            7026..7433
                     /locus_tag="PKN53_05090"
     CDS             7026..7433
                     /locus_tag="PKN53_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88364.1"
                     /translation="MKKKLFYSTVLLILIFISGCKKENVCGNMPEGLYEGWFTDYGAS
                     SVYIPLYVSKIDENTLVINSTGESSYGPFVKRDGCSIGGVIEGKSCLGEVQLKKGNYL
                     IKGTYSYLANNGGQGNPNPQYYEVHGTFEIKSN"
     gene            7758..8402
                     /locus_tag="PKN53_05095"
     CDS             7758..8402
                     /locus_tag="PKN53_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HOD88365.1"
                     /translation="MSDNRVKSNIKDITWGLQKLMQIRPVHYSIVNNMFDSSGVKIEG
                     TRNQYGFISQEIEKAFPEVKITDDVLGYKLMDYDQIIPITVAAIQEQQKTIDSLKTEL
                     ANLKDQVNSNISLGIENNNSSTLTSRTVLNQNSPNPFNESTEVTFSIDDQNFRTASIV
                     IFDMSGLLIKKYDIPKSGDGTIKINGNELKAGMYIYTLIVNQKEIDSKRMILLN"
     gene            8511..8888
                     /locus_tag="PKN53_05100"
     CDS             8511..8888
                     /locus_tag="PKN53_05100"
                     /inference="COORDINATES: protein motif:HMM:NF039701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HOD88366.1"
                     /translation="MPPSPIFNPFMVIAMPILITFAAVVLIIFLVLRYRKIREKGRYD
                     LYVKALEAGQPLPDKFFEEPAKTSSSNLRRGAVLIAVGLGMAVLGLLGKNVVMIGIGA
                     IPGFIGIAFMLIYNIERKHKKEE"
     gene            8896..9438
                     /locus_tag="PKN53_05105"
     CDS             8896..9438
                     /locus_tag="PKN53_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD88367.1"
                     /translation="MSKAEDMLLISQVVISDDRLAFDKLVRKYQSPVRRFLLNLTLGD
                     SMLADDLAQETFIKAYLNIRSFKGISAFSTWLFRIAYNTFYDSMRAKKHFEDLNTSEI
                     DKKNSSEIEYSEEKNDIYTALKLLSEEEKAVILLCYMEEMTHKEATKILKMPLGTVKT
                     HVLKSKKKMGEFLIKQGYGN"
     gene            9428..9748
                     /locus_tag="PKN53_05110"
     CDS             9428..9748
                     /locus_tag="PKN53_05110"
                     /inference="COORDINATES: protein motif:HMM:NF027794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5056 domain-containing protein"
                     /protein_id="HOD88368.1"
                     /translation="METKNDEILKDFFANNKSEIQDNGFTESVLNQLPRKKVKQAKWL
                     VPVFTLLGIFVSTLFIDFNKLISEIYFSFLQLPLFYYVCLVLTIPVVVLLLFFFAERQ
                     NKLQ"
     gene            complement(9766..10855)
                     /gene="prfB"
                     /locus_tag="PKN53_05115"
     CDS             complement(join(9766..10800,10802..10855))
                     /gene="prfB"
                     /locus_tag="PKN53_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_113942210.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOD88369.1"
                     /translation="MITTEKIKETKARIEALRGIFEIEQKREFVASESLKTQEPDFWN
                     DPEKAEKILKNITAEKKWIVEFDKLLSKYEEIELVYEFFKSDEITEEEADKEFEKFVN
                     LIEDLELKNMLRNEEDSFDAILTINSGAGGTESNDWASILERMYLRWAEDNNYKVSII
                     DRLDGDTAGIKSVTMSISGDFAYGYLKSENGVHRLVRLSPFDSQHKRHTSFASVFVYP
                     LVDDTINIEINPSEIEWDTYRSSGAGGQNVNKVETAVRLKHLPTGIVIENSETRSQLK
                     NRENAMRLLKSQLYELELRKQQEEREKLEGQKQKIEWGSQIRNYVLHPYKLVKDLRTD
                     YETGNTQAVLDGKIWDFIKAYLMEFGGK"
     gene            complement(10886..11353)
                     /locus_tag="PKN53_05120"
     CDS             complement(10886..11353)
                     /locus_tag="PKN53_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOD88370.1"
                     /translation="MTNQKLFNEIFSKAANYCSKSEKCNFDIFQYLKKQDYDQEIIEA
                     VVEKLNSENYINESRYSAAFVNDKFQFSGWGKIKIAYMLGQKQIPSSIINSSLNLIDQ
                     QEYEDQLHKILKAKIKNIKTDDKSILKEKLLRFASSRGFEIENIYKIFDILGI"
     gene            complement(11346..12149)
                     /locus_tag="PKN53_05125"
     CDS             complement(11346..12149)
                     /locus_tag="PKN53_05125"
                     /inference="COORDINATES: protein motif:HMM:NF022430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2797 domain-containing protein"
                     /protein_id="HOD88371.1"
                     /translation="MIYSGNIQKMRSIFENPVKYFLQCGDSEIELNNLIGKKIKIEYQ
                     NRINCISCGALTKTSFCQGYCYSCFTSLPQCDEGVLNPEKDMSHLGISRDMEWSKQNS
                     LIDHYVYLALTGNLKVGVTRHTQIPTRWIDQGAIEAIKLAKTPHRNLAGQIEVALKQH
                     ISDKTNWSKMLLATKHDIDLIQSKKEIMTLVPDEFKKYITLDNSITKIEYPFAPQGID
                     KFTTINFDTNPQIESKLIGIKGQYLIFENANVLNIRKHNGYFVNIEVYD"
     gene            complement(12162..12713)
                     /locus_tag="PKN53_05130"
     CDS             complement(12162..12713)
                     /locus_tag="PKN53_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HOD88372.1"
                     /translation="MILDKNSLSSAVMEAAKNIGIAIQTAPKGKGRDTLDFRIVSNKE
                     KMEEIALKMEEISEKSGQKFFLRDANNVRESDVIILVATYIESLGLRNCGFCGKENCE
                     NKNNFPEVPCAINTVDLGIAIGSAVSRAADYRIDNRVLFSAGTAVKEMGMFDEKYKVI
                     FAITLSASAKNIYFDRKPHTTNK"
     gene            complement(12710..13654)
                     /locus_tag="PKN53_05135"
     CDS             complement(12710..13654)
                     /locus_tag="PKN53_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOD88373.1"
                     /translation="MNNKVLLIDTVHEIIPEKLKKSGFTVAEAYKESKIEIQKFISEY
                     CGIVIRSRFNIDKEFIDCAKNLKFIARVGAGMESIDTDYCNKKGIVCLNSPEGNRDAV
                     GEHAVAMLLSLLNNLPKANKEMKLGIRNREENRGSELGSKVVAIIGYGNMGSSFAKKL
                     SGFGCKVIAYDKYKKNYSNQFVKETDYKEIFEKADILSLHVPLTAETKFLVNSEFIAK
                     FKKPIILINTARGQVVKTSDLVDALKSEKILAAGLDVLEYEDASFETTIDLKDNPDFQ
                     HLAKSENVILTPHIAGWTYESKTKLAEILVDKIINLKI"
     gene            complement(13737..15362)
                     /gene="pruA"
                     /locus_tag="PKN53_05140"
     CDS             complement(13737..15362)
                     /gene="pruA"
                     /locus_tag="PKN53_05140"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOD88374.1"
                     /translation="MNNALFSFPRPANEPIFQYLKGSKERELLMAELKRMSSEKIEIP
                     LIIGGKEVRTGNTVDVVMPHNNKKVIAVCHMAGEKEVEMAIEAAMAAKKQWENLPWTE
                     RASITLKIAELISTKYRPTISAATMLGQGKNIYQSEIDAVCEAADFLRFNAHYASQIY
                     ANQPGEIKNQLNRMEYRALEGFVFALTPFNFTAIASNLNMSPALMGNVTVWKPSTTAV
                     YSNYHLMKIYKEAGLPDGVVNFVPGKGSMMGKVILASKHFAGLHFTGSTATFNTLWKG
                     AANNVDKYVSYPRIVGETGGKDFIFVHKSAQADEVANAIVCGSFEYQGQKCSAASRVY
                     MPKSLWSKTKEILTKTLSTLKMGDVNDPDNFINAVIDEASFDNIMRYIQLAKDSKECE
                     IIAGGKGDKSVGYFVEPTVIVTTNPRFTTMEEEIFGPVVTIYVYEDDKYEETLEICNT
                     TSPYGLTGSIFSRDIYATQIAVDALRYAAGNFYINDKPTGAMVGLQPFGGARASGTND
                     KAGGSLNLIRWVNARAIKETFVSPTDHRYGYMK"
     gene            complement(15554..15624)
                     /locus_tag="PKN53_05145"
     tRNA            complement(15554..15624)
                     /locus_tag="PKN53_05145"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15590..15592),aa:Gln,seq:ttg)
     gene            complement(15738..16445)
                     /locus_tag="PKN53_05150"
                     /pseudo
     CDS             complement(15738..16445)
                     /locus_tag="PKN53_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833445.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            complement(17021..17623)
                     /locus_tag="PKN53_05155"
     CDS             complement(17021..17623)
                     /locus_tag="PKN53_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88375.1"
                     /translation="MEDLLKNDFTAYYDLSVCDEPNLTIQTNKPYFEIEDNALIKDIV
                     LHTKIGAGMARFSNPKSATVTVAKYEKFVTSLPNSFQRSRKRCDIIVCDNGNNLILGE
                     IKDSPNIKGHRKEAKKQLYESLITLLAVPQFIELTNLKTIKRCCYFNKQAISPTGITA
                     TTAFNRLPEIFKDGFKMNFPAIEENNFEYWEYSGNQTLTL"
     gene            complement(17601..18779)
                     /locus_tag="PKN53_05160"
     CDS             complement(17601..18779)
                     /locus_tag="PKN53_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD88376.1"
                     /translation="MKESIHIKNLGPIKDIFIDDIKPLTVLIGESGSGKSTLMKVVAL
                     FRWLYKMHNIHSYLKTSNVSKSLFNFNINAYFKNCGFEEYLKNDTEIIYTVEFSDNRK
                     YTLHFVQNELTGTSDKNLIETQDLCYNKISFISETRNIIPLWSERGASLSGGYLGFYF
                     HEVYNDFDLASENIKDLDINYLNLKLSVEKEQSIKKYYIQDPDKEYKLTLKNSSSGTQ
                     NAIPVSLIAEHFSKYFDFEEAFNRTLLNFLSKTDSLRDFKPVKNLGEIKKKLFIHIEE
                     PELSLYPDAQCELINDLISKCFVSNKNTIDLMFSTHSPYIINHLNLLIKAHDCNKLID
                     GAKIEFEKIAVYLVEEGEIEDLVAKNQRIINTNALSDTINNIYDKYNQLDKDGRLIEE
                     "
     gene            19126..20715
                     /locus_tag="PKN53_05165"
     CDS             19126..20715
                     /locus_tag="PKN53_05165"
                     /inference="COORDINATES: protein motif:HMM:NF018530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine exchanger family transporter"
                     /protein_id="HOD88377.1"
                     /translation="MDILFAPYFALFLIIILGLLIGRIKIRGISLDISAVLFVAMAYG
                     YFGLEMPADLKNIGLVLFIFTIGFQSGPAFFETFKKEGKKLVILTVLIVVSGAAVALA
                     CGMLLGYSPALTGGLFTGGITSTPGLATITELSNSGEATIGYGIAYPFGVVGVVLFVK
                     LFPRLFKMDIKKAEEKYEAENLEKYPKVISRNFIVENPDVIGKQISELNIRENTGVDI
                     SRIFQGGMAFTPNPQTILFSGSIIKAVGTEADLLKVQNLIGIPTDVEIVLNENYDIKF
                     VVVTNKKVLGKTTRELGLQSKYNVVVTRIRRSGVDFSVSSNLKLQFGDKIKIAGIKED
                     VNKVVEFLGNEASKVHEANYLPVAVGIVLGVLLGQISIKIGKFDFSLGLTGGVIIVAI
                     ILSRIRKIGPVVFSLTTVASQILRQIGLLLFLIGVGTEAGSSIVPMLKTSGVSLFLSG
                     VLITMVPMILAVILGKYILKIDVLQLLGGIPGGMTSTPGLAAASSMTKTNAPGIAYAT
                     IYPIGMIMVIICVQIMFWIMA"
     gene            complement(20712..21962)
                     /locus_tag="PKN53_05170"
     CDS             complement(20712..21962)
                     /locus_tag="PKN53_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOD88378.1"
                     /translation="MTKKRFLIILIIFLLIVAVVLFFIFKPKKQETPITEEVQEEVIP
                     EKFGIPLTGWELIEDTVRQGDYIGGILSKYGISSQQIHKVVEMSCDTFRTTLITVGNL
                     YTIFYDTIIDDVPQAKIFVYENSKMKYTLYDFRNPDTVYIKRALKQIDTITNTASGII
                     EYSLWQTMIDNGYNWDIALALSQTFAWTVDFYSLQKQDWFKVIYDEYFVDNERVGLGE
                     IKAGVFHHGGKELWAIPFVEDSVLCFYDTLGNSMRKTFMPAPLEYTHIGSRYSHARMH
                     PIHHVVTPHLAVDFSAPTGTPVYAASDGTIVIRTYGTGAGNYVKIRHNSVYSTVYMHF
                     SRFGEYQVGDRVNQGDIIGYVGSTGWSTGPHLHYEIHENGKKIDPLKFEPPPAEPVDS
                     IHMERFNKEKRKWIDDINKIKLPN"
     gene            complement(21959..23056)
                     /locus_tag="PKN53_05175"
     CDS             complement(21959..23056)
                     /locus_tag="PKN53_05175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOD88379.1"
                     /translation="MKKILAIVQHRKDRSPGQRFRLEHFIPALEQNGYEVIFSNIISK
                     KDDELFYSKGKYFAKLRILIKSFFHRLRDVRIAKDCDLIFIYREAFMLGTTYFEKKLA
                     KTKVPIIFDFDDSIWLNDTSDGNKNLAWLKRSEKTAKICGLSTLVLVGNKFLADYAKQ
                     YNSNVFILPTILNIDYHNFSKSQIKKSAVCIGWTGTTTTLKHFYTAIPVLKRIKEKYK
                     EKVYFKVIANTKTWDKDIDVKLTQWNKETEIDDLCEFDIGIMPLPNDKWSNGKCGFKG
                     LQCLSLEIPAVMSPVGVNTEIIEHGVNGFLADDDEQWFEILSKLIENPELRKEIGKNG
                     RKTIEEKYSVNVWEDKFLDLIRQTIEKKFKL"
     gene            complement(23053..>23623)
                     /locus_tag="PKN53_05180"
     CDS             complement(23053..>23623)
                     /locus_tag="PKN53_05180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD88380.1"
                     /translation="LNIAKDDFVLSYSGSIGTWYMLDEMLDFFKVLKQKYSNSKFFFI
                     TNDNPVNILTKAKEKNIDENDIIIKSADRNQVPEFLSLSTMSIFFIKPVFSKKASSPT
                     KLAEILGMGIPIVCNSNVGDIDEIFSNNNIGLVVSEFNEQEYKNTIDKLDKNFNLDKS
                     ELREVSKKLFSLQMGVDRYYNIYKSLSQK"
BASE COUNT         7850 a         3542 c         3678 g         8553 t
ORIGIN      
        1 ttggcatacg ttactatttc caaaaataaa attcaaaaac aattaaaaat ttagtatttt
       61 tgtacaactt ttttataagt gtagttaatt ttatagatta tgaaaaaaat atttatcttt
      121 ttaattttaa tttccacatt tggttatctt gtggctcaac aaaaaatcga caaagccttt
      181 ccttatctta cttgggttga tccgcaaagt ggagaaaaat cttcacaagg cgaacttttt
      241 ggctatgatg tattggtatt tactcctgtg catcagagaa tagaaaattt accaaagtca
      301 gacccctgtt ataagtcaga aaacaattta gatttttatt tggttggaag atataaaaat
      361 gaaagcatga ccgatgcagt aaatattctg tattcgccag gtccaagttg ggatccaaat
      421 tttgtaatta ccgataaaaa caataaagtt atttggaatt ttatatgcga aaatatgtgc
      481 ataaatctca caggtgtaat atatacatct ggaaatgttg acaaaatgtt tgatgagcgt
      541 tgcaaattcc agttgcaaaa taatagagta gtggaggcaa aacagcctta tctttatgta
      601 ggcattaaag ataaattatt aaaacctgta aaactttata gcgacaaaac tggtggcgaa
      661 gtaattgcaa cattgccagt tggttatgaa gtagaagttc ttttaagtga aaattatgat
      721 aaatttgcag agaatataga ttatccaata aactatttag tgcgtacatc atttggttta
      781 gtaggctggc ttagacttac aagcgaagat acttatcatt tagacccagt tattagaggt
      841 ttaggatatt taggtgatta ataaatttaa aaagatgaaa aaatatattt tgtacttagt
      901 cttaatttta gcttgtgtaa atgtaaatgc acaaagcttt aaattgccag aaaaaatgat
      961 taattctgaa gatagtttag aacctctaac agaagaggaa attagcgaaa tccagggtaa
     1021 agtagatgag ttttatcata aaatgcaaaa gcaatattct gattatgatg aaatggcaaa
     1081 gcaatattat gtagaattta gaacagatac ttttgctatt gaaattatag aaaacctaag
     1141 aagctcaaag cgaattcctg agttggaata tagcattatt gtttctgatg taaacgcagc
     1201 ttatgatgtt ttgttaaata aatattataa acttttacga gcgaatttga atgaggaaaa
     1261 acaagaaatg ttaaaacaat ctcagctaaa ttggcttaaa ttcaagacat cagaattaaa
     1321 gttatgtggt gaattattct tagaagatgg aagtataggc gaaataaaag ctagatatta
     1381 tgatactgaa ctaataaaat ctcgtacaat acgattgttt gagtatttag tggaaataag
     1441 tgcgtatgta gattgataat ggcttttttg tttcaaaaat tttactttca aaaattattt
     1501 tcaaaaaatc caagtatcta taaaaaatct ttagaaactt attggaaaat ctaaaattct
     1561 ttcaactttt aacacttcaa ctacgttcag tgcatcgctt ttaactttta actgattaat
     1621 attcatcatt tccattttca aaattctttt gtcccacatg ataagacaag gattctttaa
     1681 gtttataaaa gtctttattt ggatttacgg ttctaatttt tgttaataaa gtttcagggt
     1741 caattagttc actgaatgga ataggtttta tgtcgaaatt atcttcaacg ctaaccatat
     1801 ttccgaattt gttttcttca aataatttgt aagcaccgta gcccaacata ctgcctaaag
     1861 tcgtgtcaaa agccgagctt cttacgcaac gagtttcgta accaatttgg cgaggaatga
     1921 tttttttatt ttttcctgtt cttttgaagt attctttttt tgcggcatct gcaattagtt
     1981 tgcaaacttc tgttaatccg taataaatat ttccgtgttt gtctttttct tttgcaactt
     2041 tatcatcggg tagtttgtca accaaacctt cggcaacgca aattactcca tagtttcttt
     2101 tttctttttc tctagcaata atttggtcaa caattttctt tgcaagctca tttatatcaa
     2161 tatatttttg gtcgagaaaa tcttctacag ccagcatcat agtcgcttgt gctgaaactc
     2221 cagcggcata agttatccag ccagtttttc tgcccattag ctctattatg aaataagaat
     2281 cagtacttct agcatcttct ctaaagttga taagtgcatc tttagcagtt tctacagccg
     2341 accaataacc aaaagtccaa ggtattccaa aatagtcgtt gtcaattgtt tttggcacat
     2401 gaatagttgg aaaacccatt aaatgcaaaa agtttgcaag ttttaaggtg tcgtctccac
     2461 caattgtaat agcaacttta acatttagtg attcaaaggc gtttataata ttatttaatt
     2521 tgctgttttt ctttgggtcc tcaatatctt caactttttt aatgtcttta cctggatttg
     2581 ctctactagt ttttaaaaca actccagcat ttcctctaat tccagcaact tctctagtaa
     2641 aaactatata atgttcgcct tcttctaagg tttttaattc atcagtacca aagttttcga
     2701 tattttcaaa accttttaaa attccataga gtggaattcc tgcatttatg aaatttaacg
     2761 ccaaagatga aattacagaa tttgctccag aagcaggacc accagagaaa attacagcta
     2821 cagaatcacc agcattatat ttgtattttc ctgcattttt ataagaaatt tcaatatatt
     2881 tttgtcttgc agtttttgta tcagtttgtt ttgccatgtt tttttaatat tatttgttta
     2941 tttataaagg tgtaaattaa cacaattttt aaagattaca aaactaataa aatttattaa
     3001 gctactgatt taattaggtt tatttttatt aacatgtttg atatttttat ttaaaactta
     3061 tctaataatt ttattaacta tctacgtaaa attgaaaaat ttacattatt ttagcacaaa
     3121 attagattaa aaatatggca gtacttaaaa aaatagcagt tttaacttct ggtggtgatg
     3181 ctcccggaat gaatgccgca atcagagcgg ttgtaagacg tgcgattaaa gaagatataa
     3241 aagttgtagg cgttatgcgt ggttatgatg gtttggtaaa aggtgatttt gtacctctac
     3301 tttcaaaaag cgtttctagc ataattcaac ttggtggaac tattttaaaa actgctcgta
     3361 gtaaggagtt tatgactcca gagggaaggc aaatagctta taataattta gtagaaaatg
     3421 aaattgatgc tcttgtggta attggaggtg atggtacttt taaaggagca agaattttta
     3481 ccgaagaatt taattttcct acagttggaa tccccggaac tattgataac gacttgtttg
     3541 gtacggatat gactataggt tatgatacag cgttaaatac tgtagttgag gcagttgata
     3601 aaattagaga tacagcagct tctcatgaaa gagttttttt gattgaagta atgggacgag
     3661 acgcaggatt tatcgctctt agagcaggaa ttgcatgtgg agcagaagat gtgttaatcc
     3721 cagaaacacc atcagactta gaaggtttac atgaaaaact tactagatat atagatacta
     3781 aaaaaacttc tagcgttatt ctcgtggcag aaggcgatga agaaggaggt gctttcgaaa
     3841 taaaagaaaa aatgtcgaaa tttaaaggcg actatgaact tagagttaca gttctaggac
     3901 atattcaaag aggaggctcg ccttcagctt atgacagata cttggcaagt cgtttaggaa
     3961 ttggtgctgt agaagcattg atagatgacc aaagaagtat tatgattgga atgattaata
     4021 gagaagttgt tcatgtacct tttaataaaa caacaaaaca tcataaaact atagaccaaa
     4081 aaatggttga tattgttgag attcttaatg cttaaatatt gatgcaaaaa cctgtgtatg
     4141 tagaagtctt attaccacta gctttagaaa atactctgac ctatgttgta ccaaaagaaa
     4201 taaataatac tccaaaaata ggctcaagag taattgtaga actaggaaaa agaaaaattt
     4261 acacgggaat tatcacaaaa attcattcag aaaaaccaga ttatgaatgt aaagaaattc
     4321 tttcagtaaa agatgatttt ccaattttaa gtgaaaaaca aactgaatta ttaaactgga
     4381 tttctgatta ttattgttgc actcaaggcg aaactttagc cgctgcaatg ccgtctaaat
     4441 tattggcttc aagcgaaact caagtttggt ttaataaaaa ttgcgaatct gattattctc
     4501 caacgcaaac tgaaataaat attttaaatt atcttgaaaa tagcccgaaa gcaagcgttt
     4561 ctgatatagc aaaagctgta aatttaaaaa acccattgaa acatattgaa aatttggaag
     4621 aacaaggttt tttaaatctt tctcaacaaa ttgtaaatgc ttataaacct aaatatcaaa
     4681 attttttaag ttttaatctt gacttagctc aggaaaaact tgatgaaata gaaaaaatct
     4741 taacaaaaaa taataagcaa aaagaagtac ttcaattttt taatgaaaag tgcttacaag
     4801 aaaatattac aagtttagaa tattctgaaa aattattaat gcaagaatta aatgttgcaa
     4861 aatcaactat tcaaacgctt gtaaacaaag ggctaattga aattgtgaaa aaagaagaaa
     4921 ctcgatttga aactataaat gttgaaagtg caaaaagttt aaaactttct gaggctcaaa
     4981 ccgaagttta taatcagatt aaaaataatt ttggaaactc taaacctgtt ttgttgcatg
     5041 gtgtaacttc atctgggaaa actgaaattt atataaaatt aattaaggaa gttctaaaaa
     5101 caggtaaaca aattttgtat ttattgcccg aaattgcttt aactactcaa atgattaatc
     5161 gtttgcgtaa ttatttcggc gataaaatag gagtttttca cagtaaatat acagttgatg
     5221 cacgttcaga aatttatttg aaattattga aaaaagaatt gcaaatcgtt ttgggagtta
     5281 ggtcggcagt tttgcttcca ttctcagatt taggtttaat tattgttgac gaagaacacg
     5341 aaacaagttt taaacaacaa gatcccgacc cacgatattc ggcaagggat actgctgtag
     5401 ttttatcaaa acttcataaa gctgaaattg ttctcggttc ggcaactcct tcgatagaat
     5461 cgtataataa tgcaattaat tcaaaatatt tgcttacaac attaaaccaa agatatggaa
     5521 atgttgaaat gcctgaaatc gttttggcag atatgagcga tgcttataaa agaaaaatta
     5581 attctggaca ttttcatcca attttaatta atgaaattaa aaaggtttta gaaaaaaatc
     5641 agcaagtaat tttatttcag aatagacgag gttatacttc aattatagaa tgtagagatt
     5701 gcggttgggt gcctcattgc aaaagttgta atgtgagttt aacttatcat aaatttagaa
     5761 ataagttagt ttgtcattac tgtggcgaaa tgtacgacat tgtaaataat tgtgggaatt
     5821 gtaacagcga aaagcttagt cataaaggtt ttggaacaga aaaacttgaa gatgaggtaa
     5881 gtaaattttt tccacatgcc agaattgctc gtttagatta tgatacagca agtacaagaa
     5941 ataaattttc tcgaataatt gaaggttttg aaaaacatga atttgatatt ttagttggta
     6001 cacaaatggt taccaaaggt ttagattttc aaaaacttgc tttagttgga gttttaaatg
     6061 ccgacaattt attaaattat ccagaattta gagcttacga aagagctttc caattattaa
     6121 cacaagtaag tggtagagcg ggtaggagag ataaacgagg caaagtcatt atccaaactt
     6181 atgatgtcct aaatcctata attcaatatg tgaaaaatga agattatctt tcaatgttta
     6241 attctcaact tgctgaaaga caattgttta actatccgcc tttttcaaat tatataataa
     6301 taaaattaaa acataaagat agagctaaag tgcatagaac tgcccatgtc tttgctatga
     6361 tgttgagaac aaaacttgga aatagagtga gaggacctga agagccttta ataaataaaa
     6421 ttagcaatta ttttatttta aatgttcata ttagatttga aaaaactatt tctggcactc
     6481 agcttagaaa atttataaaa aatagaattg caaaaattaa agaagaaagt gatttttcaa
     6541 gcgtaaggtt tgaaatagac gtagatccat aaaattttta tacattattt aaaaaaaaat
     6601 cactttttca aatccaaacc tatgttttat tacgtctaat agataaacaa aatttattaa
     6661 caaattaaaa aaatgaaaat gaaaaaaatt aggataatat taaattttgc tcttgaacca
     6721 tgggctccac ccaacatcac tacaccattt ccatcagata tgcttattga ttatgttaag
     6781 gtctatgacg tcaagaaaga ttgcagcaac aacttaaatg tttgtgatta taattttggt
     6841 gtgcatgaca acaaagtaaa gaaaagcatt accattggta atggtagttg tataaattct
     6901 ttgagtgttg gccaaaatgt ttttttgaga gcttcggaag gtgttttaat caatggcgat
     6961 tttactgtac cagacggtgc acagttgtat attgatgtca atacatgtta ttaaaacttt
     7021 gggatatgaa aaaaaaacta ttttattcaa cagtactttt aatactaatt ttcattagtg
     7081 gttgtaaaaa agaaaatgta tgtggtaata tgccggaagg actttatgaa ggctggttta
     7141 ctgactatgg tgccagttcg gtatacattc ctttgtatgt atctaagata gatgaaaata
     7201 ctttggtaat taatagcaca ggggaatctt catacggacc ttttgttaaa agagatgggt
     7261 gttccattgg tggagtaata gagggtaaga gttgtttggg ggaagttcaa ttaaaaaagg
     7321 gaaattacct tattaaaggt acatactcat atcttgctaa caatgggggg caaggaaatc
     7381 ccaatcccca atattatgaa gttcatggaa cttttgaaat caagtcaaat taaagcatat
     7441 aacaatcaat catgaaaaca aaatcaataa tactctctgc aatttttgta gtttttacat
     7501 tgtcagtctt tgctcaatta aaagttgacc aatatgggag aataggcatg ggtacaaact
     7561 ggccgaatcc tgaatttaag tgtcatattg ctggaaattt acttttaacg agctatccat
     7621 ctaatccttt ttatgaatta cgaatgaaag taaacaacgg ttgaccaggt gttgaaattg
     7681 ggagcaatta tgacaaaata gcgttttggt catcttacgt tgaatataat gatctgtatg
     7741 ctgcaaatta ttacaaattg tcagataacc gtgttaagtc aaatataaaa gacattactt
     7801 ggggcttgca aaaattgatg caaattcgtc ctgttcacta ttctatcgta aataatatgt
     7861 ttgatagttc aggagtgaaa attgaaggaa ctagaaatca atatggtttt atttcacaag
     7921 agatagaaaa agcctttcca gaagtaaaaa tcacagatga tgtgcttggg tataaattga
     7981 tggattatga ccaaataata ccaattaccg tagcggccat tcaagagcaa caaaagacaa
     8041 ttgattcatt aaaaactgaa ttagcgaatt tgaaagacca agtaaactca aacatttcac
     8101 ttggtataga aaacaacaat tcatctacat taacttcacg aactgtattg aatcaaaatt
     8161 caccaaatcc ctttaatgag agtacggaag tcacattttc aatcgatgat caaaatttta
     8221 gaaccgcatc aattgtcata tttgatatga gtggtttgtt gattaaaaaa tatgacatcc
     8281 caaaaagcgg agatggtaca attaagatta atggaaatga attaaaagca ggaatgtaca
     8341 tttatacttt gattgtaaat cagaaagaaa ttgattctaa gcgaatgata cttctgaact
     8401 aaaaatttac gtttgctaac aaaaaattgg caagagtggc aggaaacgtc ttcttttgaa
     8461 tgttttagta aaactcaaag ttcctgcttc aataggaatt tacgataaaa atgccaccct
     8521 cgccaatttt caatccgttt atggtaattg ctatgcctat tctaattaca tttgcagcag
     8581 tggttcttat tatatttctt gtgttaaggt atagaaaaat tcgagaaaaa ggacgctatg
     8641 atctttatgt taaagcactt gaagctggac aaccacttcc agataagttt tttgaagaac
     8701 cagcaaaaac atcttcatct aatcttagaa gaggagctgt tttgatagct gttggattgg
     8761 gtatggctgt tcttggttta ctgggaaaaa atgttgttat gattggaatt ggtgctattc
     8821 caggatttat aggtattgct tttatgctga tttataatat tgaaagaaaa cacaaaaagg
     8881 aagaataaac agtaaatgag taaagcagaa gatatgcttt tgatttcgca ggtggttatc
     8941 agtgatgacc gccttgcgtt tgataaactt gtgcgtaaat atcaatcgcc tgtgcgtaga
     9001 tttttactaa atcttacttt gggcgattct atgcttgcag atgatttagc acaagaaact
     9061 tttatcaaag catatctgaa tatccgttct tttaaaggaa tttcagcgtt ttcgacttgg
     9121 cttttccgca tagcatacaa tactttttat gattctatgc gagcaaaaaa acattttgaa
     9181 gacttaaata caagtgaaat tgataaaaaa aacagctcag aaattgaata ttcagaagaa
     9241 aagaacgata tttacaccgc gttaaaattg ctcagcgaag aagaaaaagc agtaattctg
     9301 ctatgttata tggaagaaat gacacataaa gaagctacaa aaatactaaa aatgccactc
     9361 ggaactgtaa aaacacatgt tttgaaaagc aaaaagaaaa tgggtgaatt tttaataaaa
     9421 caaggttatg gaaactaaaa atgatgaaat attaaaagat tttttcgcta acaacaaatc
     9481 cgaaattcaa gataacggat tcactgaaag tgtgttaaat caacttccac gaaagaaagt
     9541 aaaacaggca aaatggcttg ttcctgtttt taccttgctt ggaatatttg tttctacatt
     9601 gtttattgat tttaataaac ttatttctga aatttacttt tcttttttac aacttcctct
     9661 gttttattat gtttgtttag tgctaacaat tccagttgtg gttcttttac ttttcttttt
     9721 cgctgaaaga caaaataaat tgcaataaaa ttgtaattac agtttttatt tcccaccaaa
     9781 ctccattaaa tacgccttaa tgaaatccca tattttaccg tcgagtacag cttgagtatt
     9841 tcctgtttcg taatctgtac gaaggtcttt tacaagtttg taagggtgaa gaacataatt
     9901 tcgaatttgc gaaccccatt ctattttttg tttttgtcct tcaagttttt ctctttcttc
     9961 ttgttgtttg cgaagttcta attcgtataa ttgagatttt aataacctca tggcattttc
    10021 gcgatttttt aattgagaac gtgtttcgct attttcgata acaattcctg ttggtaagtg
    10081 ctttagtcgt acagcggttt caactttatt tacattttgt ccgccagcac cgctacttct
    10141 ataggtgtcc cattcaattt ccgaaggatt tatttcaata ttaattgtgt cgtcaactaa
    10201 aggataaaca aaaaccgaag caaatgaagt atgtcttttg tgctgtgaat caaaaggcga
    10261 tagccttaca agtcggtgaa ctccattttc gctttttaaa tatccataag caaaatcgcc
    10321 acttatgctc atagtaacag atttaattcc agcggtgtcg ccgtctaatc tgtcaattat
    10381 gctaacttta tagttgttgt cttcagccca acgtaaatac attctttcta aaatcgaagc
    10441 ccagtcgttg ctttcagtgc ctccagcccc agaattaatt gtaagtattg catcaaaaga
    10501 gtcttcttcg ttgcgaagca tgtttttgag ttctaaatct tcaattaaat ttacgaattt
    10561 ttcaaactct ttatctgctt cttcttcagt aatttcgtct gatttaaaaa actcataaac
    10621 taactcaatt tcttcgtatt tggatagcaa tttatcaaat tcaactatcc attttttttc
    10681 ggctgtgatg ttttttaaaa ttttttctgc tttttctggg tcgttccaaa aatctggttc
    10741 ttgagttttt aaactttctg aagcaacaaa ttcgcgtttt tgctcaatct caaagatacc
    10801 tcctcaaagc ttctattcga gcttttgttt cttttatttt ctcagtagta atcataacaa
    10861 aatttaattt tacaaaaata ggaatttaaa tccctaatat atcaaaaatt ttataaatat
    10921 tttcaatttc aaagcctcta cttgatgcaa atcttaataa tttttctttt aaaatagatt
    10981 tatcatcagt ttttatgttt tttatttttg cttttaagat tttgtgcaat tggtcttcat
    11041 attcttgttg gtcaattaaa tttaaacttg aattaattat tgagcttggt atttgttttt
    11101 gtccgagcat atatgcaatt ttaatttttc cccagcccga aaattgaaat ttatcattaa
    11161 caaatgctgc cgaatatcta ctttcgttta tataattttc ggaatttaat ttttcaacaa
    11221 ctgcttcaat aatttcttgg tcataatctt gtttttttaa atattgaaaa atatcaaaat
    11281 tgcacttttc ggatttgcta caataatttg cagctttaga aaaaatctcg ttaaaaagtt
    11341 tttgattagt cataaacttc aatatttaca aaataaccat tgtgttttct aatgtttaaa
    11401 acatttgcat tttcaaaaat taaatattgc ccttttattc caattaattt tgattcaatt
    11461 tgaggatttg tgtcaaaatt tatggttgta aatttatcaa tgccttgtgg tgcaaatgga
    11521 tattctattt ttgtaatgct attatcgaga gtgatatatt ttttaaactc atctggaaca
    11581 agagtcataa tttctttttt tgattgaatt aaatctatgt cgtgtttagt agccaaaagc
    11641 attttcgacc agtttgtttt gtcagaaata tgttgtttta gagcaacttc tatttgtcca
    11701 gctaagttcc tatgtggagt ttttgcaagt tttattgctt ctatagctcc ttggtcaatc
    11761 cagcgagttg gaatttgagt atgacgagtt acaccaactt ttaaatttcc tgttaatgcc
    11821 aaataaacat aatgatcaat taacgagttt tgctttgacc attccatatc tctactaatt
    11881 ccaagatggg acatgtcttt ttcgggattt aaaacgcctt catcacattg aggaagactt
    11941 gtaaagcaag aatagcaata accttggcaa aaacttgttt ttgttaaagc accacaacta
    12001 atgcaattta ttcggttttg atattcaatt ttaatttttt tacctataag attgtttagc
    12061 tcaatttctg aatcgccaca ttgtaaaaaa tattttacgg ggttttcaaa aatagacctc
    12121 attttttgaa tatttcctga ataaatcata gttgttgtgt tttatttgtt tgttgtgtgt
    12181 ggttttctgt caaaataaat gttcttagca cttgcactta gagtgattgc aaaaattact
    12241 ttatattttt catcaaacat tcccatttct ttaacagcag ttccggcaga aaacagtact
    12301 ctattgtcaa ttctataatc agcggctctg ctaactgctg aaccaattgc aattcctaag
    12361 tcaacagtat ttatagcaca aggaacttcg gggaaattat ttttgttttc acaattttct
    12421 tttccacaaa aaccgcagtt tcttaatcct aaactttcta tatatgtagc tacaagaatt
    12481 ataacgtcag attctctcac attgtttgca tctcgtaaaa agaatttttg tccgcttttt
    12541 tctgaaattt cttccatttt caaagcaatt tcttccattt tttctttatt gctaacaatt
    12601 ctaaaatcga gagtgtcgcg tcctttgcct ttcggtgctg tttgaattgc aataccaata
    12661 ttttttgccg cttccattac ggcagacgaa agagagtttt tgtcaagtat catattttta
    12721 aatttataat tttatcaact aatatttcgg ctaattttgt tttcgattca taagtccaac
    12781 cagcaatatg aggtgttaga attacatttt cactttttgc aagatgttga aaatcaggat
    12841 tgtcctttaa gtctattgtt gtttcaaaag aagcatcttc gtattctaaa acatctaagc
    12901 cagctgccaa tattttttct gattttagag catcaactaa atccgaagtt tttaccacct
    12961 gaccacgagc agtatttatt aaaattattg gctttttaaa tttagctata aattctgaat
    13021 ttaccaaaaa tttggtttct gctgtcaatg gaacatgcaa acttaaaata tcagcttttt
    13081 caaaaatttc tttataatca gtctctttta caaattggtt ggaataattt tttttgtact
    13141 tatcgtaagc aataactttg caaccaaagc cagaaagttt ttttgcaaaa ctacttccca
    13201 tatttccgta cccaataatt gctacaactt tgctacctaa ttcagaacca cgattttctt
    13261 cccgattgcg aatgcctaat ttcatttctt tgttggcttt gggaagattg tttaataaac
    13321 tcagcaacat agctacagca tgttcgccaa cggcatcgcg atttccttct ggtgagttta
    13381 agcaaacaat tccttttttg ttgcaatagt ctgtgtcaat actttccatt cctgcaccaa
    13441 cacgagcaat aaattttaga ttttttgcac aatcaataaa ttctttatca atattaaatc
    13501 tgctacgaat aacaattcca caatattccg aaataaattt ttgaatttca attttacttt
    13561 ctttatatgc ttctgcaaca gtaaaaccag attttttcaa tttttctgga ataatctcat
    13621 gaacagtatc tatcaataaa actttgttat tcataattta attttattaa gctacaaata
    13681 taaaacaaaa aaaaagaccg agaaaattcg gtctttcttt tatagaattt tatatcttat
    13741 ttcatatatc catatctgtg gtcagttggg cttacgaaag tttctttaat agctcttgca
    13801 tttacccatc taattaagtt tagcgaacct ccagctttat cgttagttcc agacgctctt
    13861 gcaccaccaa atggttgtaa acctaccatt gcacctgttg gtttgtcatt aatgtagaag
    13921 ttaccagcag cgtatcttaa agcatcaaca gcaatttgag tagcgtaaat atcacggcta
    13981 aagatagaac ctgttaatcc ataaggtgaa gttgtgttac aaatttcaag tgtttcttca
    14041 tatttgtcat cttcgtaaac ataaattgta actactggac caaagatttc ttcttccata
    14101 gttgtaaatc ttggatttgt tgtaacgatt acagtaggtt caacaaaata tcctacagat
    14161 ttgtcgcctt taccaccagc aataatttca cattctttag aatcttttgc taattgaata
    14221 tatctcataa tattgtcgaa tgaagcttcg tcaataactg catttatgaa attatcagga
    14281 tcgtttacat cgcccatttt gagtgtggaa agcgtttttg ttaatatttc ttttgttttg
    14341 ctccataatg attttggcat ataaacgcga gaagctgccg aacatttttg accttgatat
    14401 tcaaaagaac cacaaactat agcattagca acttcgtcag cttgtgcaga tttgtgaaca
    14461 aagataaagt ctttacctcc agtttcacct acaattcttg gataagaaac atacttgtca
    14521 acattgtttg cagcaccttt ccacaatgtg ttaaaagttg ctgtagagcc agtaaagtgt
    14581 aagccagcaa aatgtttact tgctaaaata accttaccca tcatactacc tttaccagga
    14641 acaaagttta caactccgtc aggtaaacca gcttctttgt aaattttcat taggtgataa
    14701 tttgaataaa ctgctgtagt tgaaggtttc caaacagtaa cattacccat taaagctggc
    14761 gacatattta ggtttgaagc aatagctgtg aaattaaaag gtgttaaagc aaatacaaaa
    14821 ccttctaaag cacggtattc cattctgttt agttgatttt ttatttcacc aggttggttt
    14881 gcataaattt gagatgcata gtgagcatta aatcttaaaa aatcagcagc ttcgcaaaca
    14941 gcgtcaattt cggattggta aatattttta ccttgtccca acattgtagc agcgctaata
    15001 gtaggacgat actttgttga gataagttca gcaattttta aagtaatgct tgctctttct
    15061 gtccatggta aattttccca ttgttttttt gcagccattg ctgcttctat agccatttcg
    15121 acttcttttt caccagccat atggcaaaca gctataactt ttttattgtt atgaggcata
    15181 acaacatcaa cagtatttcc tgttctaact tcttttccac ctataattaa tggaatttca
    15241 attttttcag aactcattct tttaagctct gccattaaaa gttcacgttc tttacttcct
    15301 tttaaatact gaaaaatagg ttcgtttgcc ggtcttggaa aactaaataa tgcattgttc
    15361 ataataaaat attttaaact ttaaattttg ttaagattac aaaaataata aaattatttt
    15421 attttctaag aagaattacc atgataaaaa acatcaattt ttgaaaaaag tataaaaata
    15481 ttggaaaatc ttgggcttat taaaaacaaa aaagattgca cttaaaaatg caatcttttt
    15541 tttattcttt ttgttgtccg acaaggattc gaaccttgac aggcagaacc aaaatctgca
    15601 gtgctaccgt tacaccatcg gacaaaagaa ctttgtgagt gcaaaagtaa ttctttttaa
    15661 ttaaatacca aaaaaattgt gtaaaaaaaa cttgcacaag cagattccgt gcttatcgtt
    15721 cattatcagc agtttagcta ttcgtacatt cctgttttca atctaataac ttcttggtcg
    15781 ctaagaaatc tccatcttcc tctaggtaag tttagcttag ttaagcctgc aaaataaact
    15841 ctatccaact tttttacatc atagccaaaa tgttccataa ttctacgaac aattctattt
    15901 cttccagaat gaatttctat tcctacctca gtttttagat tgtcaacata gctaattgcg
    15961 tcaacaacaa ttggtccgtc ttctaattct attccttcag caatttggtt taaatggtcg
    16021 cgagtaagag gtttgtcgag ttctacgtgg taaattttct ttttattgta cgaagggtgg
    16081 gttaattttt cggttaattc gccatcattt gtcaataaaa gaagtccagt agtatttcta
    16141 tccaatcttc ctacaggata aactcgctct ttgcaagcat tttgaattaa atccataact
    16201 gttcgtctgc cttcggggtc gtcagatgta gttacataat ccttgggttt attgagcaaa
    16261 atataaactt tcttttcagg atttattttt ttgtcattaa atttaacttc atcgccaggc
    16321 ataactttta tgccaagctc tgtaactatt tcgccattta cactaactaa tcctgcacta
    16381 ataaaagtat ctgcttctcg tcttgagcaa ataccagcgt tagcaatata tttattaagt
    16441 ctaatacctt cttttggtgt agcttttgct gatttgtcat ttcgtctcga gggtttagaa
    16501 attttcttca tttttaaaga ccttgtatca actcctttaa tatcatcaat atcaaagctg
    16561 tccatatttc tgtttttgga cttaggtttt tcaaatttag gtttagaaat tttctttttt
    16621 aaatattctg gtccagcaaa atattttgat gctttgtcat atttttttcc gtaattttta
    16681 ccttttcttg gtgctgagtc attgtaattt cttggtcttt tgctagattt agttgtctga
    16741 ttattgttat aagccatttt aaatttaatt taaggttaat aaaaattttt taagcaaagc
    16801 aggtgcttga ataaatctaa cttgatgtgc tagaatgcaa tcttgtgttt ttctatatta
    16861 ttaacatcat taatgttata taagtagtaa tgctttttat tttttttgtc ttgtaaattt
    16921 ttgaaatcat gtcttaaagt tttatttatt taatatgttt aacgtaaaaa aaatattttt
    16981 gttttgattt taacaaaata aaaacaagta ttaaatattt ttatagcgtt aatgtttgat
    17041 tacctgaata ttcccaatat tcaaaattat tttcttcgat tgcaggaaag ttcattttaa
    17101 atccgtcttt aaatatttca ggaagtctat taaatgctgt agttgctgta attccagtag
    17161 gcgaaatagc ttgtttgttg aaataacagc atctttttat agtttttaaa tttgttaatt
    17221 ctataaattg gggaacagcc aacaaagtaa ttaatgattc ataaagttgc ttttttgctt
    17281 cttttctatg tcccttaata tttgggctgt cctttatctc tccaaggatt aaattatttc
    17341 cattatcaca tacaattata tcacatcttt ttctgcttct ttgaaaagaa ttaggtaaac
    17401 ttgttacaaa tttttcatac ttagcaactg taactgtcgc agattttgga ttggaaaatc
    17461 ttgccatacc tgctccaatt ttggtatgca acacaatatc ttttataaga gcattatctt
    17521 caatttcgaa atatggcttg tttgtttgga ttgtcaaatt tggttcatcg caaacagaaa
    17581 gatcataata agctgtaaaa tcattcttca ataagtcttc catctttgtc taattgattg
    17641 tatttgtcat aaatgttatt gattgtatcc gataaagcat ttgtgtttat aattctttga
    17701 tttttagcta ctaaatcttc aatttcccct tcttctacta ggtaaaccgc aatcttttcg
    17761 aactcaattt ttgctccatc aattaattta ttacagtcat gtgctttgat tagtaaattt
    17821 aaatgattaa taatatatgg actgtgagtt gagaacatta aatcaattgt gtttttgttt
    17881 gacacaaagc atttagaaat caaatcattt attaattcac attgagcatc tggatatagg
    17941 cttaattcag gttcttcgat atggataaat aatttctttt ttatttctcc aagattttta
    18001 acaggtttga aatctctcaa actgtctgtt ttggaaagaa agtttaataa tgttctgttg
    18061 aaagcttctt caaaatcaaa atactttgaa aagtgttcag ctattaaact aactggaata
    18121 gcattttgag ttcctgaaga actgttttta agtgttagtt tgtattcttt gtcagggtct
    18181 tgaatgtagt attttttaat agactgctct ttttcaacag acagttttaa gtttaaataa
    18241 ttaatatcaa ggtctttaat attttcagaa gccaaatcga agtcattata tacctcatga
    18301 aagtaaaaac ctaaatatcc accgctaaga gaagcacctc tctcagacca aagtggaata
    18361 atatttcttg tttcagaaat gaaacttatt ttattgtaac ataaatcttg agtttcaatc
    18421 aaatttttat cacttgttcc tgtaagttcg ttttgtacaa aatgaagagt atattttcta
    18481 ttgtcagaaa actcaaccgt atagataatc tcagtatcat tttttaggta ttcctcaaaa
    18541 ccgcaatttt taaaatatgc gtttatatta aagttaaaaa ggctttttga aacattgctt
    18601 gtttttaggt aagagtgaat attgtgcatt ttgtaaagcc atctaaacaa tgctacaact
    18661 ttcattaatg tgcttttacc acttcctgat tcacctatta aaactgttag tggtttgatg
    18721 tcatcaataa aaatgtcttt tattggacct aagtttttta tatgtattga ttctttcatt
    18781 tttatttaat attgcgtatt tataatttgt cactatgaaa ctaacataac aaagctgtta
    18841 tatagtgttt aattagtggc aaatatattt tataaaaata taaactagat tacgtagttc
    18901 aaagacgatt ttttaaattt atacaaaagc catattgttg tttacaaaca gcgatttata
    18961 tgggtgtaaa agattgtttt tgctaagatg ttaaatcttg tattatgaaa gtaatttttc
    19021 attaaaatac ttgttttata ctctaaaaca ttttaaaata tattttataa cattttaaaa
    19081 acttatttca agcagtagtt ttgcaaaatt aaatttattt attgaatgga tattcttttt
    19141 gctccatatt ttgctttatt tttaataatt attttaggtc ttttaatagg taggataaag
    19201 ataagaggca ttagtttgga catttctgca gtattgtttg tagctatggc ttatggttat
    19261 tttggattag aaatgcctgc agatttaaaa aatattggct tagtgctttt tatttttact
    19321 ataggatttc aatctggtcc agcttttttt gagactttta aaaaagaagg aaaaaaatta
    19381 gtaatattaa ctgttcttat tgttgtatct ggagctgcag tagctttagc ttgtggaatg
    19441 ttacttggct atagcccagc tttaactggc ggtttgttta caggtggaat tactagcaca
    19501 cccggacttg ctactataac agagttgtcg aattcgggcg aggcaactat aggttatggt
    19561 attgcttatc catttggggt tgttggtgtt gtgctttttg taaaactttt tcctcgttta
    19621 tttaaaatgg atattaaaaa agctgaagaa aaatatgaag ccgaaaattt agaaaaatat
    19681 cctaaagtta ttagtagaaa ttttattgta gaaaatcctg atgttattgg taaacaaata
    19741 tcagaactaa atataaggga aaatacaggt gtagatattt ctagaatttt tcaaggcgga
    19801 atggctttta caccaaaccc gcaaacaatc ttatttagtg gctctataat aaaagctgtt
    19861 ggcaccgaag ctgatttgtt aaaggtacaa aatttaatcg gcataccaac agatgtagaa
    19921 attgtgttaa atgaaaatta tgatattaaa tttgttgttg taactaataa aaaagtatta
    19981 ggtaaaacta ctagagagct tggtttgcaa agtaaataca atgttgtagt tacaagaata
    20041 agacgtagcg gagttgattt ttctgttagt tcaaatttaa aactacaatt tggtgataaa
    20101 attaaaattg ctggaataaa agaagatgta aataaagtag ttgaattttt aggaaacgag
    20161 gctagtaagg ttcatgaagc caactatttg ccagtagctg taggtatagt tttaggagtt
    20221 ttacttggac aaataagtat aaaaatagga aaatttgact tttctttagg tttaacaggt
    20281 ggagtaatta tagtagcaat aattttatca cgtattagaa aaatcggacc agtagttttt
    20341 tcgctaacaa cagtagcttc acagatttta cgacaaattg gtctattatt atttttgatt
    20401 ggcgtaggaa cagaagctgg ttcaagtatt gtgccaatgt taaaaactag tggagttagt
    20461 ttgtttttat cgggcgtttt aattaccatg gtaccaatga ttttagctgt tattttagga
    20521 aaatatattt taaaaattga tgttttacaa cttttaggcg gaataccagg cggtatgaca
    20581 agtactccag gattggcagc tgcaagctct atgacaaaaa caaatgctcc aggtattgct
    20641 tatgcaacca tttatcctat aggtatgatt atggtaataa tttgtgttca aataatgttt
    20701 tggataatgg cttagtttgg aagtttaatt ttattaatat catcaatcca tttgcgtttt
    20761 tctttattaa acctttccat gtgaatagaa tcaacaggtt ctgctggtgg tggttcaaat
    20821 ttaagtgggt ctattttttt gccattttca tgaatttcat aatgcaagtg aggaccagtt
    20881 gaccagcccg tgcttcctac ataaccaata atatctcctt gatttactct atctccaacc
    20941 tgatattctc caaatctcga aaagtgcata tatacagttg aataaacgct attatggcga
    21001 attttaacat aattacctgc accagttcca taagtacgaa taactattgt tccatcagaa
    21061 gcagcataaa caggagttcc tgtaggtgca gaaaaatcaa cagctaagtg tggcgtaaca
    21121 acatgatgaa ttggatgcat acgtgcatga gaataacgcg aacctatatg agtatattct
    21181 aatggtgctg gcataaaagt ctttcgcata ctattaccaa gtgtatcata aaaacaaaga
    21241 acagagtctt ccacaaaagg tatagcccaa agttcttttc caccgtgatg aaaaacacca
    21301 gctttaattt ctcctaagcc aactctttca ttatcaacaa aatattcgtc ataaataact
    21361 ttgaaccagt cttgtttttg aagcgaataa aaatcaactg tccaagcaaa agtttgagac
    21421 aaagccaaag caatatccca attataacca ttgtcaatca ttgtttgcca aagcgaatat
    21481 tcaataatgc ctgaggcagt gttagttatt gtgtcgattt gttttaatgc tcttttaata
    21541 taaacagtat caggattgcg aaaatcataa agtgtatatt tcattttaga attttcataa
    21601 acaaaaattt tagcctgtgg aacatcatca attatggtgt cgtaaaaaat tgtgtataaa
    21661 tttccaactg ttattagcgt tgtcctaaaa gtgtcgcatg acatttctac aactttgtgt
    21721 atttgttgag aactaattcc atatttactt aaaattccac ctatataatc gccttgacga
    21781 acagtatctt caataagttc ccaacctgtt agcggaattc caaatttttc tggaataact
    21841 tcttcttgaa cttcttctgt aataggagtt tcttgttttt taggtttaaa aataaagaat
    21901 aaaacaactg ctacaattag tagaaaaatt attaaaatta ttaaaaatcg tttcttcgtc
    21961 ataacttaaa ttttttttca atagtttgtc taatcaaatc taaaaattta tcttcccaaa
    22021 catttaccga atatttttct tcaatggttt tgcgtccatt tttcccaatt tctttgcgta
    22081 gctcagggtt ttctatgagt tttgataaaa tttcaaacca ttgttcgtca tcatctgcta
    22141 aaaaaccatt aactccatgt tcaattattt cggtatttac accaactggc gacataacag
    22201 ctggaatttc taaacttaaa cattgtaaac ctttaaaacc acatttgcca ttactccatt
    22261 tatcatttgg tagtggcatt attcctatgt caaattcgca caaatcatca atttctgttt
    22321 ctttattcca ttgtgttaat tttacgtcaa tatctttatc ccaagttttt gtgtttgcaa
    22381 ttactttaaa atatactttc tctttgtatt tttcttttat tctttttaat acaggaatgg
    22441 ctgtgtaaaa gtgttttaaa gttgtagtag tgcctgtcca gcctatgcaa actgcagatt
    22501 ttttgatttg gctttttgaa aaattgtggt agtcaatatt taagattgtt ggtaaaataa
    22561 aaacatttga gttgtactgt tttgcataat ctgccaaaaa tttatttcct actaatacaa
    22621 gtgtagataa tccgcatatt ttagcagttt tttcggatcg ttttagccaa gccaaatttt
    22681 tgtttccatc gcttgtgtcg tttaaccaaa tggaatcatc aaaatcaaaa attattggaa
    22741 cttttgtttt tgcaagtttt ttctcgaaat atgttgtgcc aagcataaag gcttcgcgat
    22801 atataaatat tagatcgcaa tcttttgcaa ttctaacatc tcttaaacga tgaaaaaaac
    22861 tttttataag tattctaagt ttagcaaaat atttaccttt tgaataaaaa agctcatcat
    22921 cttttttact gataatatta gagaaaataa cttcatagcc attttgctcc aaagcaggga
    22981 taaagtgttc aagtctaaat ctttgccctg gcgacctgtc tttgcgatgc tgaactatgg
    23041 ctaaaatctt tttcattttt gacttaaact tttgtaaata ttataatatc tatcaactcc
    23101 catttgcaaa gaaaacaatt tttttgaaac ttctcttagt tcacttttgt ctagattaaa
    23161 atttttgtct aatttatcaa tagtgttttt atattcttgt tcattaaatt cagatactac
    23221 aagccctata ttgttgttgc taaaaatttc atcaatatcg cctacgtttg aattgcaaac
    23281 aataggaatt cccatgccta atatttcggc taatttagtt ggtgaagatg cttttttaga
    23341 aaaaacaggt tttatgaaaa atatagacat tgttgacaaa cttaaaaatt caggaacttg
    23401 gtttctgtcg gcagatttaa tgataatatc attttcatca atattctttt ctttagcttt
    23461 tgtgagaatg tttacaggat tatcgtttgt tataaagaaa aatttagagt ttgaatattt
    23521 ttgttttaaa actttgaaaa aatccaacat ttcatctagc atataccaag ttccgataga
    23581 acctgagtaa gataaaacaa aatcatcttt tgcaatattt agt
//
LOCUS       DAOPNX010000033        23554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_2714_length_23554_cov_6.930082, whole genome shotgun sequence.
ACCESSION   DAOPNX010000033 DAOPNX010000000
VERSION     DAOPNX010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23554
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2714_length_23554_cov_6.930082"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..850)
                     /locus_tag="PKN53_05185"
     CDS             complement(140..850)
                     /locus_tag="PKN53_05185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOD88381.1"
                     /translation="MTKETKMFENQLVQRTWAETKSNDSWSIFKIMGEFVNGYDHFAQ
                     IGPCVSIFGSARTKPENRYYKMATETAYLLTQEGFGVITGGGPGIMEAGNKGASAGHG
                     TSAGCNIKLEFEQDANKYIDEDKLVTFDLFFVRKIMFMKYSQGFIVFPGGFGTLDELF
                     EALTLIQTKKIEKFPIILLDKEYWGQLFDWVKEVMLKREHNVSEADLDLYHIVDTPEE
                     AVKIIKDFYDKYTLKPNF"
     gene            complement(911..1837)
                     /locus_tag="PKN53_05190"
     CDS             complement(911..1837)
                     /locus_tag="PKN53_05190"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOD88382.1"
                     /translation="MQAKILKGTDASYFFFPEEKSTIISKSNYSIVYLAANLETKEKV
                     VCKQFLLMLTPNQSARLKFYIETNIRLEHKGIAKIIDLIIQNGEVFIIQEYVHGLSLK
                     ELITSREYFDYRYNYFFYKTIANFLDILTFFHSQGLCLCDVKPSNIMVCFSENSEIDM
                     KDPQVKLIDIELVKPSFKPAIPNFGGKNFSLIYASPEQILGFEELVGEHSDIFSTGLI
                     LYEAIAKEPALNTANPMFVKRIQTRVKVKKHYRFDDELFKVIYKACVKPEFSVIDKKQ
                     SEDEIKLAIVKSLSKRYQSAEEFKNDVLKLIL"
     gene            complement(1844..3301)
                     /locus_tag="PKN53_05195"
     CDS             complement(1844..3301)
                     /locus_tag="PKN53_05195"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOD88383.1"
                     /translation="MKRLLYIFLFILLTSKAFATLEVYFMYAEFNSPAGPYIETYMST
                     MGKSAVFVKNENNKFQAEIEVTMIFKSDDDKIVAFNKYNLLGPEIEDTSLAKPNFIDL
                     QRVSLDNGKYKIEVTIKDINSKEEPMMIIDEFEISFNQNEIQFSGIELIEEFEKAETT
                     SSLTKNGLDLIPYVSNFYPQNFHSFIFYAEIYNSDKVLNSDFLLRYYLSQYENSKILE
                     QYSSFKKLSSAEIVPFLGAFNIKDLPSGNYLLVLEVRDNKNELLQTKKFFFQRSNSLA
                     TNQEHINKILVDYDITANFQGSMTIKDSLYHYISCLRPIADMNEQRFIDNQLKSASLE
                     EMQHYFMEFWLKRNNVQPGELWRQYKEQVDYVDRIYQTPINRGYSSDRGRVYLQYGAP
                     DDIYISKHEPSAYPYEIWSYYKIRNENNKKFIFYNPHIAGKEYELLHSDLTGEIKTPN
                     WERLLNKRNTTLYNHDLKESDDIWGGRAKEEYKKK"
     gene            complement(3423..4214)
                     /locus_tag="PKN53_05200"
     CDS             complement(3423..4214)
                     /locus_tag="PKN53_05200"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOD88384.1"
                     /translation="MMKNLQISVEKIAKQAGEIIRENIISRTNLEIEQKGLNDFVTEI
                     DKQSEVFIVKELQKLLPASGFITEENTIQTNNKEYTWIIDPLDGTMNFIHGLYPCSVS
                     IALMKENEIILGVVYEIGLDECFSAIKNGGAFLNGKPIKVSQTQNLANSLIGTGFPYS
                     KFEKLDKYLKTLEVFIKSSRGVRRPGSAAADLSYVACGRYDGFFEYNLNSYDVAAGSL
                     IVSEAGGKLSDFSNSNNYIFGKEIIATNSLIMEEMLEIISKNFKS"
     gene            complement(4211..5512)
                     /gene="mtaB"
                     /locus_tag="PKN53_05205"
     CDS             complement(4211..5512)
                     /gene="mtaB"
                     /locus_tag="PKN53_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOD88385.1"
                     /translation="MNLSNKKVAFYTLGCKLNFAETSTISRFFTDKEIKIVKFNEIAD
                     YYVINTCSVTSNANKKSRNAIVRATKKNPNAIIIVTGCYAQLKPHEIKNICGVDYIFG
                     ANNKDDIEKIIETSSKLKKVEITTTDHKDMKSFFPAVSKGDRTRAFLKVQDGCDYFCA
                     YCTIPYARGRSRNLPIKEIIKQAEFVAANNIKEIIISGVNIGDFGKSSNETFFDLLKK
                     LVEVENIQRYRIGSIEPNLLTDEMIEFIANHKKIMPHFHIPLQAGSDEVLKKIKRKYN
                     TALFKSKIEKIKSLMPQVFIGIDLIVGVNGETKENFNETIEFVKSLDISYIHYFQYSE
                     RENTPAINFEPKVSPSEKHERAKIIDEISKSKHKSFLSSQINKKFQVLFESSKKGNKM
                     FGFTNNYIRVVSNYDKCLVNQIVDVEIIDFYDDETMQIKIL"
     gene            complement(5618..6118)
                     /locus_tag="PKN53_05210"
     CDS             complement(5618..6118)
                     /locus_tag="PKN53_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88386.1"
                     /translation="MKKLILLLVPVMMLAFACNNTAEKKTDEQQVEEKTCDKAKEECH
                     RNKMTVDSLLNNLPDYVDKEVSVCGKCTHICQHGGKRIFLASPNDLDLVIIGMANDEL
                     GSFNDTLVGENVRLKGILRAVTNEDEVETHHDIEVRYYIEVSEAKLSCCKNKKNHCGE
                     KHHENE"
     gene            complement(6146..6580)
                     /locus_tag="PKN53_05215"
     CDS             complement(6146..6580)
                     /locus_tag="PKN53_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88387.1"
                     /translation="MKPQFYKILILTIFGILISYCSYSQFNYKDTLETKDGIEISYKI
                     VNAKCNDKNSPAQIHLRLKNTNDYPTNIKFEIEYSTGFTQRYKSELIETCIPPKSTKA
                     GRASGLVFELKTNDVDIFKNEDSEWEFTIFEVEKVENCRKLK"
     gene            complement(6587..7768)
                     /locus_tag="PKN53_05220"
     CDS             complement(6587..7768)
                     /locus_tag="PKN53_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOD88388.1"
                     /translation="MRTKIFTSIIFSILLVCANAQSQTNIVNNNNEFAFKIFKQLVKT
                     DTTNVFISPISISTALSMAYDGAKGQTAKEMRKVLSFSKSQVQSHKEFCELLNFYSTQ
                     NSDLFKIVNSAVTQEKFAFLDSYISSLKDYNALIKTADFVNPVNREAARREINKWVEE
                     NTNNKIRDLVDENSLDDMTRLVLLNAIHFKADWKFAFDPKQTRQMLFFGTNRQYITSY
                     MHIREKLNFYKSDELIMLELPYKDDKAHMYVLLPNQETQIDDFCLNLDYTKFCELKNN
                     LSERLVDLQIPKFTIESKYKLKEQLIALGMPQAFSGAANFKKMNGKKNLMIDNVIHQT
                     FIEVDEVGTEAAGATAVVVRQKSAPQAEYVNCNRPFVFVITEKEKGSILFVGKFNNPK
                     M"
     gene            complement(7772..8230)
                     /gene="pyrI"
                     /locus_tag="PKN53_05225"
     CDS             complement(7772..8230)
                     /gene="pyrI"
                     /locus_tag="PKN53_05225"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOD88389.1"
                     /translation="MKGELIVKAIENGTVIDHIKAENIFKIISIIEIQDFDTQFFIGG
                     NLDSKKYGKKAIIKIADKFPSMDDINRIALIDSNAVINIIHDFKVVEKKKVELPETIT
                     AYFKCINPKCITNNENVETKFKVINKVGNVNLKCYYCDKITGHNNLEVKK"
     gene            complement(8258..9169)
                     /gene="pyrB"
                     /locus_tag="PKN53_05230"
     CDS             complement(8258..9169)
                     /gene="pyrB"
                     /locus_tag="PKN53_05230"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOD88390.1"
                     /translation="MNKKSLISITDYSKEDIFKILDLAEEFQNNPKHEKLLENFVIAS
                     LFFEPSTRTRLSFESAIQKLGGKVVGFSEASNSSVAKGESLKDTILTVSNYSDLIIMR
                     HPIEGSARYACDITSTPIINAGDGANQHPTQCLLDLYSIRKTQGTLENLNLYIVGDLK
                     YGRTVHSLLMAMSDFNPTFNFVSPVQLRIPQEYKNFLNDKGLDFYEHDQLDENIKYAD
                     IIYVTRVQRERFSDPVEYEKVKNTYVLKNKMLENTKDNLKVLHPLPRVNEIDTDVDDN
                     PKAYYFQQAENGVYARMALICHILGIK"
     gene            complement(9229..9927)
                     /locus_tag="PKN53_05235"
     CDS             complement(9229..9927)
                     /locus_tag="PKN53_05235"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009436902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOD88391.1"
                     /translation="MRNISFLLIMTMVLIGTTWSCKSQNKVDNKKIELKTKVDSASYA
                     FGVQIGANFAQQGLDTVMNMNLVLEGIRAQIAKTAIIDVNQTDKIINEFFSEMQKAQS
                     AGKLEEGEKFLAENGKREGVTTTASGLQYEVIKMGNGEKPNASSTVKVHYRGTLLDGK
                     EFDSSYARKEPISFPLSGVIKGWTEGLQYMPVGSHFKFYIPWNLAYGERGAGGLIGPY
                     ETLIFEVELLSIEK"
     gene            complement(10021..10863)
                     /locus_tag="PKN53_05240"
     CDS             complement(10021..10863)
                     /locus_tag="PKN53_05240"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOD88392.1"
                     /translation="MKTIFITGATSGIGEASAKLFLNKNWKVIASGRNQEKLELLKEL
                     GAETYRLDVTNEQDINKLFTELNEAGTKIDVVLNNAGYGQFGTIEEVSDSLAKKQFET
                     NVFGLASVCRAAIPILRNNGGGRIINVSSVAGFVSMPGGGWYAASKHSVKALSDALRW
                     ETKQFGIKVSMIEPGPIKTGFADTVNSNMVNNENGVYSSLVKDLTKSTLSFGGGTVNH
                     CAKKIYRAATRRCPKNRYMVTKEAYLIKFLIHALPPKVLDYFVIKMFIPALKVYAKTK
                     KSAN"
     gene            complement(10872..11045)
                     /locus_tag="PKN53_05245"
     CDS             complement(10872..11045)
                     /locus_tag="PKN53_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88393.1"
                     /translation="MKERVFDIKTKILKGVDLAGQKLINERAREDGEIVISKNGKIEF
                     VKAKDLINNLDIS"
     gene            complement(11048..11614)
                     /locus_tag="PKN53_05250"
     CDS             complement(11048..11614)
                     /locus_tag="PKN53_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HOD88394.1"
                     /translation="MPNLYIISGCNGAGKTTASYTILPEMLNCREFVNADEIAKGLSP
                     FQPEKAIIGAGRIMLHRIKELIKLEECFAVETTLASRTYVKFIENAKANGYFVTLVYF
                     WLNSPELAVERVKTRVKQGGHNIPTDVIYRRYKAGLKNLNNLYLDIADFWMIIDNSEF
                     PSKFIAEGNKSNVKEVYYKEIFDKIVKR"
     gene            complement(11829..12668)
                     /locus_tag="PKN53_05255"
     CDS             complement(11829..12668)
                     /locus_tag="PKN53_05255"
                     /inference="COORDINATES: protein motif:HMM:NF037635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HOD88395.1"
                     /translation="MNILGKNTKLTITEVKGDEIIFETKDFGHISAQNNDLIIKYKVG
                     QKVDVFIYPDSENTIKAFIGKAYAQVNEFAKLKVVALTKYGAFLDFGLEKDILCPFSE
                     QKTKMEMNKYYIVFIYLDEKTKKLAASAKLEKFISNENLELKENDEVDIMILNKSQLG
                     YNAIVENKHMGLLYNNEVFSELKPGETTTAIVKKIREDNKIDLRLFKNDASDISKFEQ
                     QIIEYLKKHNGKMYINDESDPEDIYKTFGISKKNFKKALGALYRKEIIDLKDTAVKLI
                     KNE"
     gene            12834..13772
                     /locus_tag="PKN53_05260"
     CDS             12834..13772
                     /locus_tag="PKN53_05260"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOD88396.1"
                     /translation="MILPENITKDFFNNIIEDKIRMHSKLLFFKGSDFNVSASFCTKL
                     AKNICAIANSGGANLIYGIETKRGKAEKFNFINNFHHSKEWLFHEIQSHIDKPIRDLN
                     INIFIFDENSSLIHFQIPENNNQPHMFSDSKYYKWQKNRAVVLDESEVRSLYGRLSVS
                     ELEFLGIYNTNGLPVLSGGKFSAMSFYPKLLIRNSGNIVEKDYKVEISFPANLYEESF
                     KPLSSLFIRHEGSYVVFGNKGMHPIFQEEISTMIEAKISVNLDNLDTFLKEYLNITLY
                     FSNGIKKHSLKLSDTLTYNGKILQKDQFANVGVLFQ"
     gene            complement(13793..15070)
                     /locus_tag="PKN53_05265"
     CDS             complement(13793..15070)
                     /locus_tag="PKN53_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD88397.1"
                     /translation="MNTKSRLIFMNFLQFFVWGAWMMTLAHYGFVEKGWSGSEFGLVF
                     STMGFASIIMPTLFGIIADKWKANYVYAILHILFGATMLILPSINSPITFFWVLLVAM
                     SFYMPTIGLTNSISFGVLKKDGKDPVVAFPPIRVWGTVGFIVAMWTTNFFTKDWGLGY
                     SIKVSFVIAAVLAFVLGIFSFIFLPTIKSEKKHDNEKKSFIQKLGLEAFVLFKQKKMA
                     LFFIFSLLIGAALQLTNAFGDAFLQDVSVFPEGSLINNFSTIILSVSQISETLFILAI
                     PFFMKRFGIKKVMLLSMIAWVLRFALFGIADNSVLGFALIILSCIIYGMAFDFFNISG
                     ALFVEKNTDSSIQSSAQGLFMLMTNGIGAVVGNNVASLVIQKWFEDPVNPHVKDWTGI
                     WLTFAAYALVVAIVFAIVFKYKHDPNEEFEVKH"
     gene            15220..16014
                     /locus_tag="PKN53_05270"
     CDS             15220..16014
                     /locus_tag="PKN53_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88398.1"
                     /translation="MKKTVFLIILTAVFATVSFGQEQEIKTKFENDSVYVDTLNRNLV
                     GLNVFPAFGILGGGRLPSTKVFIQYKHYYKHMNLRASLNYLNFDFNNDQLDVLGFTKD
                     TVVTDSGTNVVDNINFRKFYYNIYSYDARVGLEAAFPSKAYRFYLGAGAIIGYHYLGE
                     YYYHYKKNFEGYPALNVNHSFIPADISGYRKTRFLKTGVDFTIGVDINITPSFVISVQ
                     YAPELTYYHRISDDVSDKDNCFEGKKVPSSLTFVPDYIDLIFSIRF"
     gene            complement(16119..16655)
                     /locus_tag="PKN53_05275"
     CDS             complement(16119..16655)
                     /locus_tag="PKN53_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88399.1"
                     /translation="MSSCYDEKLRIENEQSLIRKAKRFGFGTNNIVSSNSDNFLHILN
                     GQGIPDAKIYDKFGNYIEFKSNDSACNAELSEFIQNLHKDTIYKTSEYPNFFEEYSKY
                     RDLKGNDLPKPNSADFYIVIFWTAWTGRLNKTHVKIWEELAKENKNCEIEVLKLNLDI
                     QNHWNKEERERIIKVFSY"
     gene            16840..20070
                     /locus_tag="PKN53_05280"
                     /pseudo
     CDS             16840..20070
                     /locus_tag="PKN53_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836643.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
     gene            20299..22119
                     /locus_tag="PKN53_05285"
     CDS             20299..22119
                     /locus_tag="PKN53_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOD88400.1"
                     /translation="MDYSFFDFLMLVGSLGLFLYGMKIMSEGLQKLAGNKLRSILSAM
                     TRNRVTGVLTGLLVTALIQSSSATTVMVVSFVNAGLLSLVQSIGVIMGANIGTTATAW
                     LISLFGFGKFSISVLSIPLMGIAIPLIFSSKSKRKSIGEFIFGFAFLFMGLDLLKSSM
                     PDLQSNPEVLAFVQNFSDMGFWSILIFLLIGTILTIVVQSSSATVALTLIMATKGWID
                     FPSAAAMIMGENIGTTITANLAAIPANIAAKRAAFAHFMFNVFGVIWMLFVFKYFVQM
                     VEYLVASYGPANPAGISDYVNSLPAEEYKQITTLSKSELPAHLVEKQDTYLNYQAATS
                     YGLTLFHTMFNICNVMIMIWFVNLYEKICLKVIRPRRGEEEDGDFGLQYITTGMLSTA
                     ELSILQAEKEVELYAKRVRKMLGFVEQMTNMNHNDKKFLKLYNRLEKYEEISDRMEIE
                     IGSYLGKVAEGRLSNESKEHIRSILREVSEIESVADACCKIGRHLKRKMDADIVFGDE
                     LVENVNSMYELLKEAFDNMQKILASATITEQELIASRSIEDAINEQRNLLKRKNIESL
                     NNLEYSYQASVYYMDIISECEQMGDYIINVIESIKIKNRG"
     gene            22444..23043
                     /locus_tag="PKN53_05290"
     CDS             22444..23043
                     /locus_tag="PKN53_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88401.1"
                     /translation="MTTVERDAIVNPAHSLLIFNTTTRCIEFYDQDNNEWGSLGCMNP
                     AYPSSGGVDYVHCSGTPTAVVDVTNPTTGKTWMDSNLGASQVATAKDDANSFGDLFQW
                     GRFADGHQCRTSNTTTTLSDSDMPGHSDFIIRTASVAPNDWRSPQNDNFWQGVSGINN
                     PCPSGYRLPTKAELEAERVSWGTNSPAGAMASPLKLQLP"
     gene            23333..23406
                     /locus_tag="PKN53_05295"
     tRNA            23333..23406
                     /locus_tag="PKN53_05295"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23367..23369,aa:Asp,seq:gtc)
BASE COUNT         7794 a         3507 c         3582 g         8671 t
ORIGIN      
        1 cttttaactg atttgctttg ctcgcttagg tttagcttcg caaagttcgc aagttcactc
       61 tgatcgctct acgcccagta gcaaccgctc attgagcgta gccgaaatga gcgtagccga
      121 aatgagcgag taattaattt taaaaattag gtttaagtgt atatttatcg tagaaatctt
      181 taatgatttt tactgcttct tctggagtat caactatatg atataagtct aagtctgctt
      241 cagaaacatt atgctctctt tttagcataa cttcttttac ccaatcaaaa agttgtcccc
      301 aatattcctt gtctagcaag atgataggga atttttcaat ttttttagtt tgaattagtg
      361 ttaaagcttc aaaaagttcg tcaagagttc caaagccacc agggaaaaca atgaaacctt
      421 gagaatattt cataaacata attttgcgaa caaagaaaag gtcgaaagta acaagcttat
      481 cttcgtcaat atatttattt gcatcttgtt cgaattctaa ttttatatta caaccagcag
      541 aagtgccatg tcctgcacta gctcctttat ttccagcttc cataatgcca ggaccaccac
      601 ctgtaattac tccaaaacct tcttgagtta ataaataagc agtttctgtt gccattttat
      661 aatacctgtt ttcaggtttt gtgcgtgcag aaccaaaaat tgaaacacaa ggacctattt
      721 gtgcaaaatg gtcgtatcca tttacaaatt ctcccattat tttaaaaata ctccacgaat
      781 cgttagattt tgtttcagcc caagtccttt gtactaattg gttctcaaac attttagttt
      841 cttttgtcat aaatatattt tttattgtaa tgtgaacaaa aatagtattt ttttataaat
      901 caacaactat ttataatatt aatttcaaga catcattttt aaattcttca gcagattgat
      961 atcgttttga tagcgatttt actatagcta attttatttc atcttcgctt tgttttttat
     1021 ctattacaga aaattcaggt tttacacagg ctttataaat aactttaaat aattcatcat
     1081 caaatctata gtgttttttt acttttactc ttgtttgtat tcttttaaca aacatagggt
     1141 ttgcagtatt taatgcaggt tcttttgcaa ttgcttcata taaaattaag cctgtgctaa
     1201 aaatatcgga atgttcgcca actagctctt cgaaaccaag aatttgttca ggactagcat
     1261 agattagact aaaatttttt cctccaaaat ttggaatagc aggtttgaag ctaggtttaa
     1321 ctaattctat atcaataagt ttaacttgtg ggtctttcat gtctatttcc gaattttctg
     1381 aaaaacaaac cattatattt gaaggcttaa catcgcacaa gcacaagcct tgggagtgaa
     1441 aaaaagttaa aatatctaaa aaattagcaa tagttttgta aaaaaagtaa ttatatctat
     1501 agtcaaaata ttctctactg gttattagct cttttaaact taaaccatgc acatattctt
     1561 gaattataaa gacttctcca ttttgaataa ttaggtcgat aattttagca attcccttat
     1621 gttctaatct aatatttgtt tcgatataaa attttagtct tgccgattga tttggtgtta
     1681 gcatgagcaa gaactgtttg catacaactt tttctttggt ttctaaatta gcagctaaat
     1741 aaacaattga ataatttgat tttgaaatta ttgttgattt ttcttcaggg aaaaagaaat
     1801 aagaggcatc tgttcctttt agaatttttg cttgcataat atattatttc tttttgtatt
     1861 cttcttttgc tctaccaccc caaatatcgt ctgattcctt taaatcgtga ttataaagtg
     1921 tggtatttct tttgtttaac aatctttccc aatttggagt ttttatttcg ccagtgaggt
     1981 cagaatgtag taattcgtat tctttaccag ctatatgagg attataaaaa ataaactttt
     2041 tattattttc gtttctaatt ttatagtagc tccatatttc ataagggtag gcactaggtt
     2101 cgtgctttga gatataaatg tcatcagggg caccatattg caaataaacc ctgcctctgt
     2161 cgcttgaata accacgattt ataggcgttt gataaattct gtcaacataa tcaacttgtt
     2221 ctttgtattg tcgccataac tcgcctggct gaacattgtt gcgttttaac caaaattcca
     2281 taaaatagtg ttgcatttct tctaaacttg cagattttaa ttgattgtca ataaatcttt
     2341 gttcgttcat atcggcaatt ggacgtaaac atgaaatata atgatacaga gaatctttta
     2401 tagtcatgct tccttggaaa tttgccgtaa tgtcataatc aactaaaatt ttgtttatgt
     2461 gttcttgatt tgttgcaaga gaattgcttc gttgaaaaaa gaatttctta gtttgtagta
     2521 attcattttt attatcgcga acttctaata caagcaaata atttccagat ggtaagtcct
     2581 ttatattaaa ggctcctaaa aacggaacaa tttctgctga actcaatttt ttaaaactgc
     2641 tatattgctc taatattttt gaattttcat attgtgatag gtaatatctt aaaagaaaat
     2701 cagaatttag cactttgtct gaattgtaaa tttcagcata aaaaataaat gaatgaaaat
     2761 tttgagggta aaagtttgaa acataaggta ttaagtctaa gccatttttt gtaagtgagc
     2821 ttgtagtttc agctttttca aattcttcta ttagttcaat tccgctaaat tgaatttcgt
     2881 tttgattaaa actaatctca aactcatcaa ttatcatcat gggttcttct ttagaattta
     2941 tgtcttttat agtaacttcg attttatatt ttccattatc caaactaact cgctgtaaat
     3001 caataaaatt tggttttgct aaacttgtgt cttcaatttc aggaccaagt aaattgtatt
     3061 tattaaatgc aacaatttta tcatcatctg atttaaaaat cattgttact tcaatttctg
     3121 cttgaaattt attattttca tttttaacaa ataccgctga ttttcccatt gtgctcatgt
     3181 aggtttcgat ataaggacca gcaggcgagt taaattctgc atacataaaa tatacttcga
     3241 gtgttgcaaa tgctttagaa gtcaatagaa taaacagaaa aatatataat aatcttttca
     3301 tactcttttt tttgcaagat acaaattttt taaactattc gtttagcttt tgtcatgatt
     3361 tttgcaaaaa ttgcaaaaat cacgccaaaa cccacattct taattcatta aaacttagta
     3421 ttttaagatt taaagttttt tgaaataatt tctaacattt cttccataat aagtgagttt
     3481 gttgcgataa tttcttttcc aaagatatag ttgtttgaat ttgaaaaatc gctaagcttt
     3541 ccgcctgctt cacttactat aaggcttcca gcagcgacat cgtaagagtt taaattatat
     3601 tcaaaaaagc catcatatct accgcaagcc acataagaaa ggtcggcagc tgctgaacca
     3661 ggtcgtctaa caccacgact agattttata aatacttcta aagtttttaa atatttgtct
     3721 aatttttcaa atttgctata ggggaaacct gttccaataa gtgaatttgc caaattttga
     3781 gtttgtgaaa ctttgatagg ttttccattt agaaaagctc caccattttt tattgcagaa
     3841 aaacattcgt ctaagccaat ttcataaaca acacctaaaa taatttcgtt ttctttcatt
     3901 agagcaatac tcacagagca ggggtagagc ccatgaataa aattcatagt tccgtctagt
     3961 gggtcaatta tccaagtgta ttctttattg ttagtttgaa tagtgttttc ttctgttata
     4021 aaacccgaag ctggcaaaag tttttgcaat tcttttacaa taaaaacttc agattgttta
     4081 tcaatttctg taacaaaatc gtttaagcct ttttgttcaa tttctaagtt agttctagaa
     4141 ataatatttt ctcgaataat ttctccagct tgctttgcaa tcttttcaac agatatttgt
     4201 aagtttttca tcataatatt tttatttgca tagtttcatc atcgtaaaaa tcaataattt
     4261 ccacatcgac tatttggttt acaaggcatt tgtcgtaatt tgaaactact ctgatatagt
     4321 tatttgtaaa tccaaacatt ttatttcctt tttttgagga ttcgaataaa acttggaatt
     4381 ttttgttgat ttgagatgat aaaaacgatt tgtgttttga tttagatatt tcatcaatta
     4441 ttttagctct ctcatgcttt tcacttgggc ttactttggg ttcaaaattt attgcaggcg
     4501 tgttttctct ttctgaatat tggaaataat gtatatatga aatatctaaa gactttacaa
     4561 attcaatagt ttcattaaaa ttttctttag tttcgccatt tacgccaaca attaaatcta
     4621 ttccaataaa aacctgaggc attaaagatt ttattttttc aatttttgat ttaaaaaggg
     4681 cagtattata ttttcttttt atttttttca gaacttcatc gcttcctgct tgtaaaggaa
     4741 tatgaaaatg aggcataatt tttttatgat ttgcaatgaa ctcaatcatt tcgtctgtta
     4801 atagattggg ctcaatgctt ccaattctgt atctttgaat attttcgact tctacaagtt
     4861 tttttagtaa atcaaaaaat gtttcgttac ttgattttcc aaagtctcca atatttacgc
     4921 ctgatataat aatttcttta atattatttg ctgcaacaaa ttctgcctgt tttataattt
     4981 ctttaattgg taaatttcgg cttcgtcctc tagcgtaagg aattgtacag taagcacaaa
     5041 aataatcgca accatcttga acttttagaa aagctctagt tctgtcgcct tttgatacgg
     5101 caggaaaaaa acttttcata tctttatggt cggtagtagt aatttcaacc tttttaagtt
     5161 tagaagaagt ttctattatt ttttcaatat catctttatt gtttgctcca aaaatataat
     5221 caacgccaca aatattttta atttcatgag gttttagttg tgcatagcaa cctgtaacta
     5281 taataatagc atttggattt ttctttgtgg ctctaacaat tgcatttctg ctttttttat
     5341 tagcatttga agttactgaa catgtgttaa ttacataata atcagctatt tcgttaaatt
     5401 ttacaatttt tatttcttta tcagtaaaaa accttgaaat agttgaagtc tctgcaaagt
     5461 ttaacttaca gccaagggta taaaatgcaa cttttttatt tgacaaattc atttaatatg
     5521 taataaaaaa gcgatgtctc aaaagcattc agcaattatc ttgcattaga acacctttaa
     5581 gacatcgttt aaatttttgt cttagaacaa ttgtttttta ttcattttcg tgatgttttt
     5641 cgccacaatg atttttcttg ttcttgcagc agctaagttt agcttcacta acttcgatat
     5701 agtatctaac ttctatatcg tgatgagttt caacttcatc ttcattagtt acagctctta
     5761 agattccttt taatctaaca ttttcaccaa ctaaggtgtc gttgaaactt ccaagttcgt
     5821 cgtttgccat accaattatt actaaatcta aatcatttgg actagcaagg aaaattcttt
     5881 taccaccgtg ttggcaaata tgagtacatt ttccacaaac gctaacttct ttatcaacat
     5941 aatcaggaag attattcaac agagaatcaa ctgtcatttt attgcgatga cactcttctt
     6001 ttgctttatc acatgttttt tcctcaactt gttgttcgtc agtctttttt tctgcagtat
     6061 tattacatgc aaatgccagc atcataactg gaactaataa taaaattaac tttttcatct
     6121 ttttaaaatt taattagtaa ttattttatt tcaattttct acaattttca actttttcta
     6181 cttcaaatat agtaaattcc cattcagaat cttcattttt aaaaatatca acatcattgg
     6241 tttttaactc aaatactaaa ccgcttgctc tacctgcttt tgtgcttttt ggcggaatac
     6301 aagtttctat taattcactt ttatatcttt gagtaaaacc tgttgaatat tctatttcaa
     6361 atttaatatt tgttggataa tcgttggtgt tttttaatct taaatgaatt tgtgcgggac
     6421 tatttttgtc attacattta gcattaacaa ttttataact gatttcaatt ccatctttag
     6481 tttctaaagt gtctttataa ttaaattgag aataactgca atagctaatt aaaattccaa
     6541 aaattgtgag tattaatatt ttataaaatt gtggtttcat ataaaattac attttagggt
     6601 tattaaattt tccaacaaat aggatagagc ctttttcttt ttctgtaatg acaaaaacaa
     6661 atggacgatt acagtttaca tattcggctt gaggtgcaga tttttgtcta acaaccacag
     6721 cagtagctcc agcagcttcg gtaccaactt cgtcaacttc aataaaagtt tgatgtatta
     6781 cattatcaat cataagattt tttttgccat tcattttttt gaaattagca gcacccgaaa
     6841 atgcttgagg cattcctaaa gcaataagtt gttcttttaa tttgtatttg ctttctattg
     6901 taaactttgg aatttgtaaa tctacaagcc tttctgacaa gttatttttt aattcacaaa
     6961 atttagtata atctaaattt aagcaaaaat catcaatttg cgtttcttga tttggtaaca
     7021 atacatacat gtgagcttta tcgtctttat aaggcaattc aagcattatt aattcgtctg
     7081 atttgtaaaa atttagcttt tctcttatgt gcatatatga tgttatatat tggcgatttg
     7141 ttccaaaaaa caacatttgt cttgtttgtt ttgggtcaaa agcaaatttc caatcagctt
     7201 taaaatgaat tgcatttaat aaaactagtc gagtcatgtc gtctaaagag ttttcatcaa
     7261 ctaaatctct aattttatta tttgtatttt cttcaaccca tttatttatt tcgcggcgtg
     7321 cagcttctct gttgacagga ttgacaaaat cggcagtttt tattaaggca ttataatctt
     7381 ttagtgatga aatataagag tctaaaaaag caaatttctc ttgtgtaaca gctgaattga
     7441 cgattttgaa taaatcagaa ttttgagtgg aataaaaatt taaaagttcg caaaattctt
     7501 tatgagattg aacttgactt tttgaaaagc taagaacttt tctcatttct tttgcagttt
     7561 gtcctttagc tccatcataa gccattgata atgcagttga aatgcttatc gggctaatga
     7621 aaacatttgt agtatcggtt ttaactagtt gtttgaaaat tttaaatgca aattcattat
     7681 tattatttac gatattagtc tgtgattgcg catttgcaca aactagcaaa atactaaaaa
     7741 tgatagaggt aaagattttc gttctcatat tttatttttt tacttccaaa ttattatgtc
     7801 cagtaatttt atcgcaataa taacatttta aattgacatt tccaacttta ttgataactt
     7861 taaatttagt ttctacattt tcgttgtttg ttatgcactt tggatttata catttaaaat
     7921 atgctgttat cgtttcggga agttcaactt ttttcttttc aacaacttta aaatcatgaa
     7981 taatatttat tactgcattt gaatcaataa gtgcgattct gttaatatcg tccatagatg
     8041 gaaatttgtc ggctattttt ataatagctt tttttccata tttttttgag tctaaatttc
     8101 cgcctataaa gaattgagta tcaaagtctt gaatttcaat tattgaaata attttgaaaa
     8161 tattttcagc tttaatatgg tcaattactg ttccattttc tattgctttt actattaatt
     8221 cgcctttcat attttatgtt tttaaaaatt atacaattta ttttatccca agtatatggc
     8281 agattagtgc cattcttgca tatacaccat tttcggcttg ttgaaaataa tatgcttttg
     8341 gattatcgtc cacatcagtg tcaatttcgt taactcttgg tagtgggtgc aaaactttaa
     8401 gattatcttt tgtattttca agcattttgt ttttaagcac ataagtattt ttcacctttt
     8461 catattctac agggtctgaa aatctttctc tttgtactct tgtaacatat ataatatcgg
     8521 catatttaat attttcgtca agttggtcgt gttcgtaaaa atcaagtcct ttatcgttta
     8581 aaaagttttt gtattcttgc ggtattctaa gttgaacagg tgaaacaaaa ttaaaagtag
     8641 ggttaaaatc agacattgcc ataagtagag aatgtactgt gcgtccatat tttaaatcgc
     8701 ccacaatata aagatttaga ttttctaaag tgccttgagt tttgcgaata gagtagaggt
     8761 cgagcaaaca ttgggtagga tgttgattag ctccgtctcc agcatttata ataggagtag
     8821 atgttatgtc gcaggcatat ctagcacttc cttctattgg atgtcgcatg ataattaagt
     8881 cggaataatt actaacagtt agaatggtgt cttttagcga ttcgccttta gcaacgcttg
     8941 agttagatgc ctcgctaaat cctacaactt ttccgcctaa tttttggata gcagattcaa
     9001 agctcaatcg agttctggtt gatggttcaa aaaataatga agcaatcaca aaattttcta
     9061 ataatttttc gtgttttgga ttgttttgaa actcttcggc taagtctaat attttaaaaa
     9121 tatcttcctt gctgtagtct gtgattgaaa ttaaactttt tttattcatc ttaatttgtt
     9181 tttatattat tcaaaaaaaa ccgaagcgta aacttcggtt ttttttaatt atttttcaat
     9241 tgacaataat tcaacttcaa aaatgagtgt ttcgtaagga ccaataagtc ctcctgcacc
     9301 tctttcgcca taagctaaat tccaaggaat ataaaattta aagtgtgaac ctacaggcat
     9361 gtattgtagt ccttctgtcc aacctttaat aactccactt aatgggaaag aaataggctc
     9421 tttacgtgca taagaagaat caaactcttt gccgtctagc aaagttcctc tataatgaac
     9481 ttttacagta cttgaagcat taggtttttc gccattgccc atttttataa cttcgtattg
     9541 caatccacta gcagtagttg taacaccttc tcttttgcca ttttctgcaa ggaatttttc
     9601 accttcttct aattttccag cagattgtgc tttttgcatt tcactaaaaa attcattaat
     9661 aattttatct gtttggttta catcaataat tgcagttttt gcaatttgag ctcttatgcc
     9721 ttctagcact aagttcatgt tcattactgt gtctaaacct tgctgagcaa aatttgctcc
     9781 aatttgaact ccgaaagcat agcttgcaga gtcaacctta gtttttaatt caattttttt
     9841 attatccact ttattttgcg atttgcatga ccaagttgta cctataagta ccatagtcat
     9901 aattagtaaa aaagaaatat ttctcatgct atataatttt agttgtgcaa aataacaaca
     9961 atatttttgt aattacaatt tttatttgat ttttcttttt atttttttct taacaaaaaa
    10021 ttaatttgca gatttcttag tttttgcata aacctttaaa gcaggaataa acattttaat
    10081 aacaaaataa tcaagtactt tgggaggtag agcatgaatt aaaaatttaa ttaaatatgc
    10141 ttcttttgtt accatatatc tgtttttggg gcatcttctt gttgctgctc tgtagatttt
    10201 tttagcacag tgattcacag ttccgccacc aaatgatagt gttgattttg ttaaatcttt
    10261 tactaaacta gagtaaactc cgttttcgtt atttaccata ttagaattga cagtatctgc
    10321 gaaacctgtt tttataggtc caggttctat cattgatact tttatgccga attgttttgt
    10381 ttcccaacgc aaggcatcgc taagcgcttt tacagaatgt ttacttgcag cataccagcc
    10441 tccaccgggc atagatacaa aaccagctac cgaagataca tttataattc ttcctcctcc
    10501 attatttctt aaaattggaa ttgcagctct acaaaccgat gctaatccaa aaacatttgt
    10561 ttcaaattgt tttttagcga gagagtcgga gacttcttca atagtcccaa actgaccata
    10621 tccagcattg tttaaaacta cgtcaatctt agttcctgcc tcgtttagtt cggtgaataa
    10681 cttgttgata tcttgttcat tagttacatc taagcggtaa gtttctgcac caagttcttt
    10741 aagtaattct aatttttctt gatttctgcc acttgctatt actttccaat ttttgtttag
    10801 aaataatttt gcactagctt ctccaattcc gctagtagca cctgtaataa agatagtttt
    10861 cattgaaaaa attaagaaat gtctaaatta ttaattaaat cttttgcttt tacaaattcg
    10921 attttaccgt ttttagaaat tacaatttca ccgtcttcgc gggctctttc gttaatgagc
    10981 ttttgtccag ctaaatcaac gcctttcaaa atttttgttt taatgtcaaa aactctttct
    11041 ttcatattta tctttttaca attttatcaa aaatttcttt ataataaact tctttcacat
    11101 tacttttatt gccttctgca ataaatttgc ttggaaactc tgaattatcg ataatcatcc
    11161 agaagtctgc aatatctaaa tataaattat tcaaattttt taatccagct ttatatcttc
    11221 tataaattac gtctgttgga atattatgtc caccttgttt tacgcgagtt tttactcttt
    11281 ctacagctaa ttcgggacta tttaaccaaa agtaaactaa agtaacaaaa tagccattag
    11341 ctttagcatt ttcaataaat tttacgtaag ttcttgaagc taaggtagtt tctacagcaa
    11401 aacactcttc taactttatt aattccttta tgcggtgtaa cattattctt cctgcaccta
    11461 ttatggcttt ttcgggttga aaaggcgaaa gaccttttgc tatttcgtct gcatttacaa
    11521 actctctaca gtttagcatt tctggcaaaa ttgtgtaaga agccgttgtc tttcctgcac
    11581 cattgcaacc tgatataata tataaattag gcatctaata ttttttccaa atacaaatat
    11641 acattaatat aagttggttt gcaattatat attacagaaa tttattaaca cttcacaaga
    11701 aattagcaat tctaaattaa aaattcttca attatcaatt ttctaaggag cgagtgcaga
    11761 gtcaatcttg cttgagccat gccgagagac gacagaaaat gtagcgaagc taaattatca
    11821 attatcaatt attcattctt aattaatttc acagcggtat ccttcaaatc aataatttcc
    11881 ttcctatata atgccccaag tgctttttta aaattctttt ttgaaattcc aaaagtttta
    11941 taaatatcct cagggtcgga ttcatcgttg atgtacattt tgccattatg ttttttcagg
    12001 tattctataa tttgttgttc aaattttgaa atgtctgaag catcattttt aaataaacgc
    12061 aaatctattt tattatcttc tctaattttt tttacaattg ctgttgttgt ttcgcctggt
    12121 ttaagttcgc taaagacttc gttattatac aacaatccca tgtgtttatt ctcaacaata
    12181 gcattatatc caagttgcga tttatttaaa atcataatat caacttcatc gttttctttg
    12241 agttcaaggt tctcgttact aataaatttc tcaagttttg cagatgcagc aagttttttg
    12301 gttttttcgt caagataaat aaaaactata tagtatttat tcatttccat tttagttttt
    12361 tgttcagaaa aagggcataa aatatctttt tctaatccaa aatctaaaaa agcaccgtat
    12421 tttgtaagtg ctacaacttt taattttgca aattcattaa cttgtgcata agcttttcct
    12481 ataaatgctt taattgtgtt ttcactatct ggataaataa aaacatcaac tttttgacct
    12541 actttatatt taataattaa atcgttgttt tgagcagata tgtgtccaaa atcttttgtt
    12601 tcaaatatta tttcgtcacc ttttacttct gtaatggtta attttgtatt ttttcctaaa
    12661 atattcatct taaatttgtt cgttaaatga tagtgtaaat aagtttgcaa aaatcatatt
    12721 tttttaaata aataacaaaa aaagttcaaa ttttaaaact tgaacaactt ttaacttttt
    12781 aacttttaac ttttaactgt gtttttttaa ctgaatttgt ttatttttgt cccatgattt
    12841 tgcctgaaaa tattacaaaa gattttttca ataatattat tgaagataaa attagaatgc
    12901 attcaaaatt gttgtttttt aaaggcagtg attttaatgt gtctgcgagt ttttgtacta
    12961 aacttgcaaa aaacatttgt gctattgcaa attcgggcgg agcaaatcta atttatggaa
    13021 ttgaaacaaa aaggggcaag gctgaaaaat ttaattttat taataatttt caccactcaa
    13081 aagaatggtt attccacgaa attcaatctc atattgacaa gccaatcaga gatttgaata
    13141 taaacatatt tatttttgac gaaaattcga gtttaattca ttttcaaatt ccagaaaaca
    13201 acaatcaacc tcatatgttt tctgattcaa aatattataa atggcaaaaa aatcgagctg
    13261 ttgtactcga tgaaagcgaa gtcagaagtt tatatggaag attaagtgtt tcagaacttg
    13321 aatttcttgg aatttataat accaatggtt tgcctgtttt aagtggtgga aaattttctg
    13381 caatgagctt ttatccaaaa ctcttgatac gcaattcagg caatattgtt gagaaagatt
    13441 ataaagttga aatttctttt cctgctaatt tatacgaaga aagttttaaa ccgctaagct
    13501 ctctttttat tcgtcacgaa ggatcttacg tggtgtttgg aaataaagga atgcatccaa
    13561 tttttcaaga agaaatttca actatgattg aagcgaaaat ttctgtaaat ctcgacaatt
    13621 tagacacctt cttaaaagaa tatttgaata ttactcttta ttttagcaat ggaataaaaa
    13681 aacatagcct aaaacttagc gataccttaa catataatgg aaaaatcttg caaaaagacc
    13741 aattcgcaaa tgtaggcgtt ttgtttcaat aagataatta cttaaaaaat cattaatgtt
    13801 ttacttcaaa ttcttcgttt ggatcatgtt tatacttaaa gactatagcg aagacaattg
    13861 caacgactaa ggcatatgct gcaaatgtta accaaattcc ggtccaatct ttaacatgag
    13921 gattaacagg atcttcaaac catttttgga taactaaact tgctacatta tttccaacaa
    13981 cagcaccaat accatttgtc ataagcataa acaaaccttg agctgatgat tgtatagaag
    14041 aatctgtatt tttttcgaca aataaagcac cagaaatatt gaaaaagtcg aaagccattc
    14101 cataaataat acaagataga attattaacg caaagcctaa aacagaattg tctgctattc
    14161 caaatagtgc gaaacgtaaa acccaagcaa tcatacttaa aagcattact ttctttattc
    14221 caaatctttt cataaaaaat ggaatagcaa gaataaataa tgtttcggat atttgagata
    14281 cagaaagtat tattgtagag aaattattta ttaaactgcc ttcaggaaat accgacacgt
    14341 cttgcaaaaa agcatcgccg aatgcattag taagttgtaa agcagcacct attaataaac
    14401 tgaaaataaa aaacaaagcc attttctttt gtttaaaaag cacaaaagct tctaagccaa
    14461 gtttttgaat aaacgacttt ttctcattgt cgtgtttctt ttcacttttt atagttggta
    14521 aaaatatgaa agaaaaaata cctaaaacaa atgctaaaac tgcagcaata acaaaagaca
    14581 cttttatgct atatccaagt ccccagtctt ttgtgaaaaa gttggtagtc cacattgcaa
    14641 caataaaacc tacagttccc catacacgaa ttggtgggaa agctacaact gggtcttttc
    14701 cgtctttttt tagaactcca aaactaatgg aatttgtaag acctatagtt ggcatataaa
    14761 aactcattgc cacaagcaat acccagaaaa acgtgatagg agaatttatt gatggtaaaa
    14821 tcaacattgt cgctccaaat aaaatatgca aaatagcata cacgtaattt gctttccatt
    14881 tatcggcaat aattccaaaa agtgtaggca taataataga agcaaagccc atggtagaaa
    14941 aaaccaaacc aaattcagac ccactccagc ctttttcaac aaaaccataa tgtgccaatg
    15001 tcatcatcca agctccccaa acaaaaaatt ggagaaaatt cataaaaatc aagcgagatt
    15061 tagtattcat atctaaattt ttaattttaa atcaataagg tttaatatgt attggcaaaa
    15121 atagagaaag tttttttatt tttagcaagt ttttactttt tatataattt tattgttaaa
    15181 tttgcgttta ataatttaag ttaattaaat atttgtgata tgaaaaaaac tgttttttta
    15241 atcattttaa ctgcggtttt tgcgacagtt tcttttgggc aagaacaaga aattaaaaca
    15301 aaatttgaaa atgatagcgt ttatgttgat actttaaata gaaacttggt tggtttgaat
    15361 gtttttccgg catttggaat tttaggtgga ggacgtttgc cgagtacaaa agtttttatt
    15421 caatataagc attattacaa gcatatgaat ttgagagcta gtcttaacta tttgaatttt
    15481 gactttaata atgaccaact tgatgtatta ggctttacaa aagatacagt tgtaaccgat
    15541 tctggcacta atgtagttga taatattaat tttagaaaat tttattataa tatttactca
    15601 tacgatgcga gagttggttt agaagctgct ttcccaagta aagcttatag gttttatcta
    15661 ggtgcaggtg caattatagg ttatcattat ctaggtgagt attattatca ttataaaaag
    15721 aactttgaag gctatccagc acttaatgta aatcattctt ttattccagc cgatatttca
    15781 ggttatagaa aaacaagatt cttgaaaaca ggcgttgatt ttactatagg cgttgatatt
    15841 aatataacac caagttttgt tatttctgtt cagtacgcac cagaattaac atattatcat
    15901 agaatctctg atgatgtttc tgacaaagac aactgctttg aagggaaaaa agtaccaagt
    15961 agcttgactt ttgtgccgga ttatatagat ttgattttta gtattaggtt ctaagacttg
    16021 gagttttggt gtgtcgcgtc ctaataaaag ttaacacatt ttgttataaa aaccgacttt
    16081 aaaaacttta aactttagga actttaaaaa ctaaacactc aataactaaa aactttaatt
    16141 attctttctc tttcctcttt gttccaatgg ttttgtatat caagattaag ctttagaact
    16201 tcgatttcac agtttttatt ttcttttgca agttcttccc aaattttaac atgtgttttg
    16261 ttaagtctgc ctgtccatgc tgtccaaaaa ataacaatat aaaaatcagc agaatttggc
    16321 ttgggtaaat cattgccttt taaatctctg tattttgagt attcttcaaa aaagttggga
    16381 tattctgaag ttttgtaaat agtatctttg tgtaaatttt gaataaattc agaaagctcg
    16441 gcgttgcaag cagaatcatt agacttaaat tcaatataat ttccgaattt atcataaatt
    16501 ttagcatctg gaattccttg cccgtttaat atatgtagaa aattatcaga gtttgaactg
    16561 acaatattat ttgtaccaaa gccaaatctt ttagcttttc ttattaaact ttgctcattc
    16621 tcaattctga gtttttcatc ataacaggac gacaagaact aagcaaaata gcaaatatta
    16681 acaataaaat atgtgatttt ttcattcagt ataaaaacat ataataatgc aaaattataa
    16741 atatttttaa cgattttaca gtttgcaata atttattgga aaaattatga actcatttaa
    16801 agctaattct tatttttgta aaaaaacagt tttaaaatta tggtaaaaaa atatttatta
    16861 agtattttgt gtttgagttt tgtgataaat cttttttcac aaaaacaatc tgaacgtttt
    16921 gcagaaatta caaatcctaa tttagttcac attaataaaa tgccagcaca tgcaagcttt
    16981 gtttcatact ctaaaatgac tgatgctcaa aattttgtgc gtgctaatga tttagaaaat
    17041 tatatttttc taaatggagt ttggaaatat cattatgtaa ataattttga cgatagattg
    17101 caaaacgatt tttataaatt ctcgtttaat gataataatt ggtcggatat aaatgtccca
    17161 ggaaattggg aatttcaagg ttttggtaca cctatttatg taaatgcaac ttatgaattt
    17221 acttcatggg gttttccacc atattgggat agaccaaatc caccacttgt gccaaaagaa
    17281 ttaaacgcaa caggaactta tcgtaaggag tttgaaatcc ccgcagaatg ggagggaaaa
    17341 gatattattt tgtcatttga tggaattaaa ggtgcttctt acttttattt gaatgaagaa
    17401 tttctcggaa tgagcaaaga tagtaaattg cctgtgcgtt ttgatattac agaaaaagct
    17461 aaaattggaa aaaatatttt ggcaattcag gttcatagat agagcgatgc aacttattta
    17521 gaatgtcaag atttttggag agtgtcggga atagaacgcg atgtttatat ttatgccaga
    17581 ccaaaagttc atatctcgga tttttttgca aaagcgattt tagacgaaaa ttatgaaaat
    17641 ggattattgg atttaacagt taaaatcaat aatttagaaa atgacaaatc aacttataaa
    17701 cttaattatc agttatttga tgataaaaat aagaaagtcg ctgaagaaac taaagaattg
    17761 agttttgttg ataatgttgc agaaataaat tttttaaaaa ctattcaaaa agtaaaaaaa
    17821 tggtcggctg aagaaccaaa tctttacact ttaatcttag aaattaaaaa cgaaaacaac
    17881 gaaagtattg aaattaccag taaaaaaata ggttttagaa ctgttgaatt aaaaaacaaa
    17941 caattacttg tgaatggcaa accaattttg attaaaggtg taaatattca cgaacatagc
    18001 gaaaaaacag gacattatgt taatgaagaa ttattgcgaa aagattttga actttttaga
    18061 aaatacaatg taaacactgc tcgtacagca cattatccac agcctgaatt gttttatcaa
    18121 cttgctgatg aatatgggat ttatgtaatt gatgaagcaa atatcgaatc tcatggaatg
    18181 ggctatagct tgcaagtagg cggttgtttg gcaaataatt tagagttttt agaatctcat
    18241 ttacatagaa caataggaat gttcgaaaga gacaaaaatc atccaagtgt aataatttgg
    18301 tctctaggaa atgaggcagg aaatggatat aatatgtatc aaacttattt atggttaaaa
    18361 aaacatgatg atactcgcct tgtacagtac gaaagagctg gtttagaatg gaatactgat
    18421 gttttttgtc caatgtatgt gccacctgcc gaaaccgaag cctatgcttt gagaacaaac
    18481 gcaaatcgtc ctttaattca atgtgaatat gcacatgcaa tgggaaatag cttggggaat
    18541 tttatagatt attggaatat cattagaaaa taccctttgt tgcaaggcgg ttgtatttgg
    18601 gattgggttg accaaggtat tgaagagaaa aatgaaaaag gcgaaaaaat ctgggctttt
    18661 ggtggcgatt acggtgaaag aggaactcca tcggctggag atttttgtat aaatggattg
    18721 atttttccag acagaacaac aaagccacat accgaagaaa tgcgtaaagt ttatcaaaat
    18781 gtgtggttcc aagattttga ttataatgct gaaactgtaa cgatttacaa cgaaaatttc
    18841 tttatagatt taagtcaata taaaattgaa tatgaaattc tttcagctgg taaaattttg
    18901 aaaacaggac aattaaatgt tgacgtaaaa ccacaagaaa gaaaaactgt ttcaattcct
    18961 ggaattaaga aattatttaa aaaagatgtt caaactttga ttaattttta tgtaaaacaa
    19021 aaagatgaag ttcgcggtat tccaaaggat tggattattg caagagacca atttatagta
    19081 aatgaatttc caaaatttga acttaaaaat caaaaaaata atttgaattt agttgatgaa
    19141 ttaggaaagt tgcaagtagt tggaaaagat tttgttgtcg aaattaataa agaaacagga
    19201 attattttgt catataaata tcaaaattta gaatttataa aagatgagtt tggtttgcgt
    19261 cctaattttt ggcgtgcgcc tttagataac gactatggag caggaatgcc taaaaagtta
    19321 gaggtttgga aaaatgcaag ttatgataaa ttaaatgtac aaaatctcga aattaaaaat
    19381 gaaatatcag agcctttaaa aattaaatta acctacaatt ttgaagaggc aaatgcaact
    19441 tgggatttag aatatacaat ttataataac ggaattatta aagttaataa taattttgat
    19501 gcacgaaaat caagtttgcc atatctgttt agactaggaa tgcgtatgca aatgccagcc
    19561 gagtttgttg atgtcgaata ttatggtaga ggtccttggg ctaactattg tgatagaaaa
    19621 acttcaactt ttattggaaa atattcttca aaaatttcgg aatttaacga aaaatatgtt
    19681 tttgcacaag aaactggaca tcatacagat acgcgttggt tggcaatttg taatcaactt
    19741 ggcaaaggct tagtttttca tgctgctaat gaaaattttg aatttaatgt ttcagatgtt
    19801 ttaatggaaa gtattgataa tggagatgat tggcataacg atgctccaag attaacagcg
    19861 gttgataaaa aacatattga tgcttatcaa aaatctgata gggtagatgt gtttattgat
    19921 tataaaatgc agggagttgg tggaaataat tcttggggag ccttgcccga agaagaatac
    19981 agacttgtgc caaaaaatct aaatgttgaa tatggatttt atttaattcc ttttgaaaac
    20041 accaaagaat taagtaaaat tttgaaataa attctcattc tttcttttgc ttttaactta
    20101 ctgagtatta gtggtttggt aaatgtttgt aaaatcttat taacaatgat ttttatattg
    20161 ttgaaaaaaa ataatgatta ctactttctt gttgtttgaa tagtttgagg cattttatta
    20221 ttaaaattat agattttcca tttgtattta tttctttttt aaatactttt gtcagcatgt
    20281 taacttatta tttttgagat ggattatagt ttttttgatt ttttaatgct cgttggttct
    20341 ttaggtctgt ttctgtacgg aatgaagata atgagcgagg gattgcaaaa acttgcaggg
    20401 aacaaattgc gttcaatttt gtcagcgatg acaagaaatc gagtaacggg tgtacttacc
    20461 ggacttttag taacagcact catacagtca tcgtctgcta ccacagtaat ggttgttagt
    20521 ttcgtaaatg caggactgct tagcttagtg cagtcaattg gtgtaataat gggtgcaaat
    20581 attggaacaa ctgccactgc ttggttgatt tctttatttg gatttgggaa gttttcaatt
    20641 agtgtcctct ctattccatt aatgggtata gcaattccgc taattttttc atctaaaagt
    20701 aaaagaaaat caatagggga atttattttt ggttttgctt tcctttttat gggacttgat
    20761 ttgttaaaat cttcaatgcc tgatttacaa agcaatccag aagtgttggc ttttgtgcaa
    20821 aacttttccg acatgggctt ttggtctata ttgatttttt tactaatagg tactatttta
    20881 actattgtcg ttcaatcatc tagtgcaaca gtagccctta cgcttattat ggctacaaaa
    20941 ggttggatag actttccatc agcagcagca atgattatgg gcgaaaatat tggaacaact
    21001 attactgcta atttagctgc aattccagca aatatagctg caaaacgagc tgcctttgca
    21061 cattttatgt ttaatgtctt tggggtaata tggatgctgt ttgtattcaa atattttgtt
    21121 caaatggttg aatatttggt agcaagttat ggaccagcaa atcctgcagg aatatcagac
    21181 tatgtaaatt ctttacctgc cgaagaatac aaacaaataa ctacactctc aaaatctgaa
    21241 cttccagctc atttagtaga aaagcaagat acttacctaa attatcaagc agcaacttct
    21301 tacggtttaa ctctatttca taccatgttc aacatatgta atgtgatgat aatgatatgg
    21361 tttgtgaatt tgtacgaaaa aatctgcctt aaagttatac gtcctcgaag gggagaagag
    21421 gaagatggag attttggact tcaatatatt acaacaggaa tgctctcaac agcagaattg
    21481 tcaattcttc aagctgaaaa agaagttgag ctttacgcta aacgagtgcg taaaatgttg
    21541 ggttttgttg agcaaatgac aaatatgaac cataatgata aaaaattcct taaattatat
    21601 aatagattag aaaagtacga agagataagc gatagaatgg aaatagaaat aggttcatat
    21661 ttaggtaaag ttgctgaagg aagattgagc aacgaaagta aagaacatat tcgttcaatt
    21721 ttacgtgaag tttcagaaat tgaaagtgtc gccgacgctt gttgcaaaat tggtcgccat
    21781 ttaaaacgta aaatggacgc agacatcgtg tttggagacg aattggtgga aaatgtcaac
    21841 tctatgtatg aacttttaaa agaagctttt gataatatgc aaaaaatatt ggcttcggct
    21901 acaataacag aacaagaact tattgcaagt agaagcatag aagatgcaat aaacgagcaa
    21961 cgaaatttat taaaacgtaa aaatattgaa agcctaaata atcttgaata ttcatatcaa
    22021 gctagtgttt attatatgga tattattagc gagtgtgaac aaatgggcga ctatattatt
    22081 aacgttattg aatcaataaa aataaaaaat agaggttaaa aaatatttta taaggctgtt
    22141 ttaaatttta ttttaaaaca gccttgtata gatattagac taattttgtt agaattgctt
    22201 ttaatattaa gtaaaaatta ttatatttgt aaatattctg attttagtaa aaaaagatat
    22261 ttaataatta gtattatgag tttgacaaaa atttaaaaaa aagcattatt attttttatt
    22321 ttagtttttt ctttttctgt tatttatgca cagggtgtag caattaatac tacaggagaa
    22381 acggctgatg agtcggcaat attagatgtt aagagtacaa cttcaggatt gttattacct
    22441 cgtatgacaa cagtagaaag agatgctatt gtcaatcctg ctcattcttt gttgattttt
    22501 aataccacaa caagatgtat tgaattttat gaccaagata ataatgaatg gggctctttg
    22561 ggttgtatga atccagcata tcctagttct ggtggagttg actatgtgca ttgttctggt
    22621 acgccaacag cagttgttga tgtaactaat cctacaactg gcaaaacatg gatggatagt
    22681 aatcttggtg catcacaagt agcaactgct aaagacgatg ctaattcttt cggagatttg
    22741 ttccaatggg ggcgatttgc agatggacat caatgtagaa cttcaaatac aactactaca
    22801 ttaagtgata gcgacatgcc tggtcatagt gattttataa taagaactgc ctctgtagct
    22861 cctaatgatt ggcgttctcc tcaaaatgac aatttttggc agggagttag tggtattaat
    22921 aatccttgcc caagtggtta ccgcttgcca accaaagcag aactggaggc tgagcgagta
    22981 agctggggaa ctaacagccc tgctggagct atggcttctc ctttaaaatt acaattacca
    23041 tgatttcgta ataatgctac ctttaatgct ggtactataa cgagtgcggg agctgtcggt
    23101 acttattggt ctagtacagt aaattctata aatgcagatc gtttgtatat ctataatgct
    23161 ggtgcttaca caaacagccc tacttatcgt ttacagggtt catcagtacg ttgcattaaa
    23221 gactaagtgg ttgataatta tgcagataag tttaatctga aaaaataatt taaaaaaagt
    23281 aaaaaatatt tggtggatat aaatatctgt tttatatttg caggcgattt aaggattggt
    23341 agttcagttg gttagaatac cggcctgtca cgccgggggc cgcgggttcg agtcccgtcc
    23401 agtccgccca aaaagctcac aacaagtggg cttttttgtt ttgtaaaaat tatcgaaaaa
    23461 attaagcata ataaaaaaac ttttttaact ttgtaggatt ataatggtcc ccttttttag
    23521 gacaacaaaa ttaaaaattt aacgtataaa taat
//
LOCUS       DAOPNX010000034        23424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_2735_length_23424_cov_6.832342, whole genome shotgun sequence.
ACCESSION   DAOPNX010000034 DAOPNX010000000
VERSION     DAOPNX010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23424
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2735_length_23424_cov_6.832342"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..149
                     /locus_tag="PKN53_05300"
     tRNA            78..149
                     /locus_tag="PKN53_05300"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:110..112,aa:Thr,seq:ggt)
     gene            211..1398
                     /gene="tuf"
                     /locus_tag="PKN53_05305"
     CDS             211..1398
                     /gene="tuf"
                     /locus_tag="PKN53_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOD88402.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLSAV
                     MSFDSIDNAPEEKERGITINTSHVEYQTASRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     IIVVAATDGPMPQTREHILLARQVNVPRIVVFLNKVDLVDDEELLELVEMEVRDLLNF
                     YGFEGDDCPVIRGSALGALNGEPQWEDKVMELMEAVDTWIPIPPRDNEKPFLMPVEDV
                     FSITGRGTVATGRIETGVINTGNEVHIIGLGEEARKTVVTGVEMFRKILDKGEAGDNV
                     GLLLRGVDKDEVKRGQVIAQPGTIKPHTRFKAEVYILKKEEGGRHTPFHDKYRPQFFL
                     RTLDITGEIHLPEGVQMVMPGDNVSIEVELIYPVACNVGLRFAIREGGRTVGAGQITE
                     IIE"
     gene            1449..1521
                     /locus_tag="PKN53_05310"
     tRNA            1449..1521
                     /locus_tag="PKN53_05310"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Trp,seq:cca)
     gene            1538..1732
                     /gene="secE"
                     /locus_tag="PKN53_05315"
     CDS             1538..1732
                     /gene="secE"
                     /locus_tag="PKN53_05315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOD88403.1"
                     /translation="MRKIKEYFKDTYNELVHKVTWPKWSELQGSAVVVMVASLIFALI
                     IFVMDYSFKNLLEVIYGIFY"
     gene            1750..2295
                     /gene="nusG"
                     /locus_tag="PKN53_05320"
     CDS             1750..2295
                     /gene="nusG"
                     /locus_tag="PKN53_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOD88404.1"
                     /translation="MSEIEKKWYVVRALSGKEKKVKEYLEAEIARLGLQDQVSQVLIP
                     TEKVYQVRNGKPITKERNFFPGYILIHAALDGEIEHSIKNVSGVIGFLKTQDGHAIPL
                     RNDEVARILGKVDELAESDEEVNMLFYVGEPVKVIDGPFSGFSGVIEEVYDEKKKVKV
                     MVKIFGRRTPLELRFIQVEKE"
     gene            2318..2761
                     /gene="rplK"
                     /locus_tag="PKN53_05325"
     CDS             2318..2761
                     /gene="rplK"
                     /locus_tag="PKN53_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOD88405.1"
                     /translation="MAKQISGFIKLQVKGGVANPSPPLGPALGSKGLNIMEFCKQFNG
                     RTQDQVGKVLPVIITYYSDKSFDFIVKTPPVAIQLLEQSKVNKGSAEPNRNKVGSVTW
                     DQVKAIAEAKMVDLNAFTLDSAMSMVAGTARSMGISVKGQSPIKK"
     gene            2779..3471
                     /gene="rplA"
                     /locus_tag="PKN53_05330"
     CDS             2779..3471
                     /gene="rplA"
                     /locus_tag="PKN53_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOD88406.1"
                     /translation="MAKISKNRKNALAKLEKGKKYDLQEAAKLVKEITFTKFDASVDI
                     DVRLGVDPRKANQMVRGITTLPHGTGKEVRVLVLCTPDKEQEAKDAGADYVGLDNYIE
                     QLSGGWTDIDIIITMPQVMAKLGKLGRILGPRGLMPNPKAGTVTMEIGKAVEDVKKGK
                     IDFKVDKYGIIHAGIGKVSFSPEKIVDNATEIIQTIIKLKPTATKGTYVRSIYLSSTM
                     SPGIEIDLKSIN"
     gene            3492..4010
                     /gene="rplJ"
                     /locus_tag="PKN53_05335"
     CDS             3492..4010
                     /gene="rplJ"
                     /locus_tag="PKN53_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOD88407.1"
                     /translation="MKREDKNALIESLKDTINEYNHFYLTDISGLNAADTFELRKKCF
                     EKGIELIVVKNTFFKQALERSGRDFQELYGVLKENTTVMFTNVANVPGKLIQELNKKS
                     DKPLFKGAYAEESFYIGADQLSALASLKSKEELIGDIILLLQSPIKTVLGQLESGKNL
                     LGGLVKTLSERE"
     gene            4047..4427
                     /gene="rplL"
                     /locus_tag="PKN53_05340"
     CDS             4047..4427
                     /gene="rplL"
                     /locus_tag="PKN53_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOD88408.1"
                     /translation="MADLKKLAEELVNLTVKDVNELANILKEEYGIEPAAAAVAVAGP
                     VAGGAGEVAEAEKTEFDVILKSAGAQKLQIVKLVKELTGLGLKEAKALVDEAPKPIKE
                     GVPKDEANALKTQLEEAGAEVELK"
     gene            4523..8326
                     /gene="rpoB"
                     /locus_tag="PKN53_05345"
     CDS             4523..8326
                     /gene="rpoB"
                     /locus_tag="PKN53_05345"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOD88409.1"
                     /translation="MISNKKQRINFSTTKNQLEYPDFLEIQIKSFEEFFQLNSTAEQR
                     KSEGLYQVFMDNFPINDTRNNFVLEFLDYTLDAPRYSIQECVERGLTYSVPLKAKLKL
                     YCTDTEHEDFETSVQDVWLGAVPYMTPSGTFVINGAERVIVSQLHRSPGVFFSQSIHT
                     NGTKLYSARIIPFKGSWIEFATDINSVMYAYIDRKKKLPVTTLLRAIGFESDKDILEI
                     FDLADEIKVSKTALKRVVGRRLAARVLKTWIEDFVDEDTAEVVSIERNEVVIDREIVL
                     EPQHIDLILESGTKTILLHKENQKVSDYAIIFNTLQKDPCNSEKEAVVSIYRQLRSSE
                     PPDEATARDVIDKLFFSDKRYDLGDVGRFRMNKKLNLNIPDEIRILTKEDIIEIIKYL
                     IELVNSKADVDDIDHLSNRRVRTVGEQLANQFGVGLARMARTIRERMNVRDNEIFHPI
                     DLINSKTLSSVINSFFGTNQLSQFMDQINPLAEITHKRRLSALGPGGLSRERAGFEVR
                     DVHYTHYGRLCPIETPEGPNIGLISSLCVYAKINKLGFIETPYRKVVDGKVCFDDIVY
                     LSAEEEEEKVIAQANALIDDDGNFLSSRVKSRDEGDFVVTDPQQIELMDVAPNQIASI
                     AASLIPFLEHDDANRALMGSNMMRQAVPLLRAEAPIVGTGIEEMVARDSRILITAENN
                     GVVEYVDAEKIIVKYDRTEEEEFLSFTDNFVTYNIPKFQKTNQNTCVNLRPLVKKGDK
                     VKKGDLLTEGYGTEKGELALGRNLKVAFMPWKGYNFEDAIVLNENIVKEDYFTSIHIA
                     DYQLEVRETKRGLEELTSDIPNVSETATKDLDENGIIRVGAYVKPGDIIIGKITPKGE
                     SDPSPEEKLLRAIFGEKAGDVKDASLKASPSTSGVVIDKKLFSKTIKDRKLRNQDKIL
                     VENLDKKHAEESAALKSMLIEKLFTLVNGKTSQGVKDNFNNDLIPKGTKFTQKLLAEL
                     DFINVSPGRWTTDKNRNETISKLLFNYMVEQKKIDGDYRRKKLEITIGDELPAGIIQL
                     AKVYIASKCVVKVGDKFAGRHGNKGILAKIVRQEDMPFLEDGTPVDIVLNPLGVPSRM
                     NLGQIYETILGWAGSVLGCRFSTPIFDGANIDQINEYTKKAGLPEYGSTYLYDGGTGD
                     RFDQPATVGVIYMLKLRHMVEDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWA
                     LEAFGAANILQEILTVKSDDVVGRAKTYEAIVKGEQMPEPGIPESLNVLVHELRGLCL
                     RVDLD"
     gene            8353..12651
                     /gene="rpoC"
                     /locus_tag="PKN53_05350"
     CDS             8353..12651
                     /gene="rpoC"
                     /locus_tag="PKN53_05350"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOD88410.1"
                     /translation="MAFRKDKKIKTKFSKITISLASSEEIQEMSSGEVTKPETINYRT
                     YKPERDGLFCERIFGPVKDYECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERIGHIK
                     LEVPVAHIWYFKTLPNKIGYLLGLPSKKLDSVIYYERYIVVQPGVKAIDGIQELDFLT
                     EDEYLEILDSLPRENQLLEDTDPNKFIAKMGAEAIQDLLKRIDLDQLSYSLRDKASNE
                     TSQQRKSEALKRLNVVEAFRLSADRNKPEWMIINVIPVIPPELRPLVPLDGGRFATSD
                     LNDLYRRVIIRNNRLKRLIEIKAPEVILRNEKRMLQEAVDSLFDNSRKSNAVKTQANR
                     PLKSLSDSLKGKQGRFRQNLLGKRVDFSARSVIVVGPDLKMHECGLPKEMAAELYKPF
                     IIRKLTERGIVKTVKSAKKIIERKDPVIWDILENVLKGHPVLLNRAPTLHRLGIQAFQ
                     PKLIEGKAIQLHPLVCTAFNADFDGDQMAVHLPLGNAAILEAQVLMLASHNILNPANG
                     APITVPSQDMVLGLYYLTKGRKGVKGEGYTYYSPEEVIIANNEGQLDLHANIKVRVDD
                     VNEKGEAVTRMIETTTGRVLFNQIVPKEYGYINEILTKKSLRDIIGNLVGRFGTDVTS
                     RFLDDIKDLGFSHAFKGGLSFNLNDVIIPETKQELIQEGYAEVDEIMFNYENGFITDT
                     ERYNQVIDVWTNINARLSIDVMKKLSTDDQGFNAVYMMLDSGARGSKEQISQLCGMRG
                     LMAKPQKSGVATSEIIENPILANFKEGLSILEYFVSTHGARKGLADTALKTADAGYLT
                     RRLVDVAQDVIITEEDCGTLRGLVAVELKKNNEEVIESLYERILGRTSVHDIYHPQTE
                     ELIVAAGEEITERIAKIIDESPIEKVEIRSVLTCESRKGVCAKCYGRNLATHRMVQKG
                     EAVGVIAAQSIGEPGTQLTLRTFHMGGVAGKIAGESFIDAKYDGILEIDELRTVEHKE
                     KDGSLVDIVVTHMAEMKILDPNTKIPLVNKPIPYGSKLYFKQGNSVKKGDRICDWDLY
                     NIVIVSEVSGKLVFEDVIEGVNYKDELIIQTGLTEKIIIETKDKTKIPVAKIVDKNNE
                     VLATYNLPVGARLQIEENEKVESGDILIKMPRIVGKGGDITGGLPRVTELFEARNPSN
                     PAVVSEIDGEVSFGKIKRGNREIEITAKDGKVNKYLVPLSKQILVQPNDYVKAGTPLI
                     DGAITPSDILAIKGPTEVQEYLVNEIQDVYRLQGVKINDKHFEVIVRQMMRKVEVVEP
                     GDTQFLEKQLVDKWEFREENDAIFDKKIVVDPGDSTNFSKGQMISYRRLRDENSILKR
                     KDMKLVEVRDALPATSSQVLQGITRAALQTNSFMSAASFQETTKVLNEAAIRAKVDEL
                     EGLKENVIVGHKIPGGTGQKEFENIRVTSKMIAEQYLNQDEISIEEEKFEETEI"
     gene            12657..12974
                     /locus_tag="PKN53_05355"
     CDS             12657..12974
                     /locus_tag="PKN53_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOD88411.1"
                     /translation="MDEKKQNQINIELSEEVGQGVYSNLAVITHSSSEFVLDFVRIMP
                     GMPKAKVQSRIILTPEHAKRLLGALQENVVKFESINGEIKISNNKPGTPIPMNFGGPT
                     AQA"
     gene            13109..13633
                     /locus_tag="PKN53_05360"
     CDS             13109..13633
                     /locus_tag="PKN53_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88412.1"
                     /translation="MKYLVEIRRKRKNDGIAIILFLLLFSFSSCFKPQTFPKEPNIEF
                     IDFIVKDTFDILQNRVLNGTLHFYFVDGDGDIGYDTTSPRQNTIFLQKYKIENKQEVL
                     INMQVPLEYFVPKFKRNIQDEALQGEMFVKDLNETFPFENDTIMYKFYIKDRAGNKSN
                     VASTGYIIVNNYLQ"
     gene            13697..15637
                     /gene="dnaG"
                     /locus_tag="PKN53_05365"
     CDS             13697..15637
                     /gene="dnaG"
                     /locus_tag="PKN53_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOD88413.1"
                     /translation="MIDQVTVQKIIDAAEITSLIQEFVSLKRRGANYLGLCPFHNEKT
                     PSFTVSPSKGIFKCFGCGKAGNAVSFIMAHEQLSYPEALRYLAKKYNIHIEEKELTEE
                     DIRANTERESMNIVNEAACKYFENTLFNSDEGISIGLSYFKERGFNEQVIKEFRLGYS
                     LEQKNAFTEWALKNGYKKEFLESTGLSFFKENYYFDRFAARVMFPILSIAGQVVGFGA
                     RTLSNDKKTAKYLNSPESLVYNKSKVVYGLFHAKNEIIKQNKKCYLVEGYMDVISLFM
                     TGIKNVVSSSGTSLTEDQIRQIHRFADDITILYDGDLAGIKASLRAIDMILEQGLNVR
                     IVLFPEGEDPDSYAKKHGSSKTIEYIEKNETDFISFKANLLFDEAKNDPIKRASLITD
                     IVRSIAIINDNIKQSIYIQECSKILDVDQSVLYAEVLKIRRSKIESIKKQEYRYSQPV
                     KLTPSLPDSFEGIYFESQEKEIIRLLLLYGNNIIDIKDGATETELEISVAQFIINELR
                     SEGLEFKNVINKKIFLFAEELINNDEPIKIELFTHNLDPEISQVSVNLCSSKYELSPI
                     WEKRGSPILKEEKRLQEILEDVFIKYKLKIAKEFETNVKNKIKLLTESKNDEELQSTL
                     EDFKKIKGILKDLSNMQGRVIL"
     gene            complement(15683..18319)
                     /locus_tag="PKN53_05370"
     CDS             complement(15683..18319)
                     /locus_tag="PKN53_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOD88414.1"
                     /translation="MNMQKTVFLSLLMLVNVLAHTQELKPDRTSINYFYDAEAFMYEG
                     NYDEALDLLLKLDNISPDNPNIWYKIGVCYLNTRLYKTQALEYLKKASNYTSPNYKEN
                     NHKEKNTPLESYLYLGIAYRVNYMFQESLDSFDKLKGMLSPSKTEDKALLKIIKREIE
                     ITENAIYNVANPIKAELINLGEVINSEFTDHSPIVDLYENYLIFTSRRPREGQEMKNQ
                     DEDIFISKKVNNKWETPIRLGEPVNTIDNNEAAIGLSIDSKQLFFYRSGENNSGSIFI
                     TESDDMYNWSTPTLLKENVNSRFKETHATISPDGNAIFFTSNRKGGFGGLDIYVMRKL
                     PNGKWSEALPLPKQINTEYDEESPFIHPDGITLYFSSKGHNSMGGYDVFSSIMSRNGK
                     FTEPVNIGYPINTPDDDVSYVVNLDGQKGYIATIKADDGYGDLDLYEILQEDVHKNNI
                     IIYEGIIADKNNNLPENVTITLKNKDSNEEPKVSRLNQNNSKYVLTMIPDNTYEIIYQ
                     IHGHTIRSIDYTPTIDELTQQTKLYNPIELDPIILSSFYNHAYVYFDENDNNLNDDAI
                     SLLDKVIEKYVEFEAKNEKLAINIVTPKQDANPLYDEKRSKEIFEYLSKNNIAKNDIY
                     SDGVFDNNSTNIYGLEIVENKKLLAIEEKEEVIVDKAILKVENILFDFDKYNIKPEYF
                     DNLDLLANYLNDNPTAKIEIGGHTDWLGTSEYNYLLSYHRSKAVKDYLVAKGNNAENI
                     ITAKYGKHTPIAANQTPDGRDNPTGRKYNRRAEFKVLVQGNKTILEILPIVIEEGDYI
                     DISKTPSNKKYTVQIYALKNQKALETFSELVGVKMHVSSDGWYRYYVGEYTSYKMAVE
                     AATNLKSMGYDTLIRKLSFYEE"
     gene            complement(18327..20231)
                     /gene="dxs"
                     /locus_tag="PKN53_05375"
     CDS             complement(18327..20231)
                     /gene="dxs"
                     /locus_tag="PKN53_05375"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOD88415.1"
                     /translation="MIDDKKYQLLKDINSPDDLKKIDLSELPTVCEELRNFIIESASK
                     NPGHFGASLGVVELTVALHYAYNTPVDQLVWDVGHQAYGHKILTGRRDLFHTNRTYGG
                     ISGFPSPKESEYDNFGVGHASTSISAALGIAKAANLQGEKINTVAIIGDGSLTGGIAF
                     EGLNNLGASGENVLIVLNDNKIAIDENRGALKEYLTDIATSKTYNKVKDDVWYFLGRL
                     NRLGLNVQSFSQKIDNAIKSIVLKNSNLFEALNIRYFGPVDGHDVVLLVKLFEDIKNI
                     PGPKLLHIITKKGKGFKAAEENQTLWHAAPGKFDITTGKLTDTSHCENEPQKFQDIFG
                     DAIIELAKANPKICAITPAMISGSSLCKMFEEFPERSFDVGISEQHAVTFAAGLAIKG
                     MLPFCNIYSSFMQRAYDQVIHDVALQNLKVIFCLDRAGLVGQDGPTHHGSFDLSYMRC
                     VPNLVIAAPRDEFQLRNLMFTLQLEKHNFPSVIRYPRGKSTQAKSKIEFKEIEIGKGE
                     KIKDGKDIAILSIGTPGNTAIKLANDLEEENLNIAVFDMIFVKPLDNKLLTEIFNNYK
                     HIITIEDNSIIGGFGSAISEFMVDNKFNSKIYRFGIPDRFISHGSQQQLIEECEFDYN
                     TLKLFIKNLN"
     gene            complement(20323..21012)
                     /locus_tag="PKN53_05380"
     CDS             complement(20323..21012)
                     /locus_tag="PKN53_05380"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HOD88416.1"
                     /translation="MIKHSKIFFTCIALSLVVSTTNAQEVAVTNDIGSWLTAGFEKKL
                     YKGFKLEFEQELRLFKNFTKIDDIISNIEFSYKINKNFSIAAGGRYTYDMDYLNNFQH
                     DLRYNLDFSYQIKLSSVIRLGYRLRYQNEYLNCFRSREIDNLQEIKFRNRIKLEYYHK
                     QANKFYFSAEIFRLHKIFRLPYFSKIRLFIGDKYSFGKHSLDISLGFHHFLKKESPAK
                     LGVLSLKYSFN"
     gene            complement(21009..21782)
                     /locus_tag="PKN53_05385"
     CDS             complement(21009..21782)
                     /locus_tag="PKN53_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOD88417.1"
                     /translation="MKQVSKSKISFINSLKLKKYRIKEKLFVVEGEKIVSEVLKSNLD
                     IKLICGSPEYLEKNEYNLEKFQDLVFEAKPEEMKKISSFVTPSNVLAIVSMPESRNIL
                     YKNEDLYLALDEIQDPGNLGTIIRICDWFGIKNLICSLNSADLYSPKTLQATMGAFLR
                     VNVIYANIAEEIVKFKNNTKNHVFGTFLNGENIYKSSLPKAGFIIMGNEGKGINKNLE
                     AMVDKKLFIPSFSELDIHAESLNVSVASAIICSEFKRNL"
     gene            complement(21898..22407)
                     /locus_tag="PKN53_05390"
     CDS             complement(21898..22407)
                     /locus_tag="PKN53_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOD88418.1"
                     /translation="MKIGLLSDTHGYLDKEILDFFAECDEIWHAGDIGSEQILDDLEK
                     FKPIKAVWGNIDGTKIRARTKEFLNFVCEGQKVLITHIGGYPGKYAPNIKNLLLDIKP
                     DIFIAGHSHILKVIYDNNLKLLHLNPGAAGNTGWHKFITAIRFEIENNTVNPNEVIKN
                     IDIFEKKRS"
     gene            22736..23347
                     /locus_tag="PKN53_05395"
     CDS             22736..23347
                     /locus_tag="PKN53_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88419.1"
                     /translation="MEANFTVKEAIIESIKLGLKNFLSLIGAVILWGLTIWIPYLNVG
                     TTIGMITLPAKMSRGAIISPTEIFASKYRKYMGEFFLVTGLKSIIMFPAFLFMYFPGL
                     VLQYAFSFATLFVVDKGINPSEAISLSNKCTYGYKWKMFFTHLVLFVVIWIVFLILGF
                     ITPILGVIAFVLFMPILLGATAFFYGKLTAHVPSADNPEIVEN"
BASE COUNT         8290 a         3342 c         4081 g         7711 t
ORIGIN      
        1 ggaatagctc agttggtaga gcatcagcct tccaagctga gggtcgcgag ttcgagtctc
       61 gtttcccgct cgaaggtgcc aatgtagctc aggggtagag cacttccttg gtaaggaaga
      121 ggtcatgggt tcaattccca tcattggctc aattttttgt aagtataaat taaaaaaagt
      181 ttaattatta atatttaata tttttaagcc atggctaaag aaaaatttga taggtcgaaa
      241 ccgcacgtaa atattggtac aattggtcac gttgaccatg gtaaaaccac cttaactgct
      301 gctattacaa cagttttagg aaaaaaaggt ttgtcggctg taatgagctt tgattctatt
      361 gacaatgctc cagaagaaaa agaaagaggt attactatta atacttcaca tgttgaatac
      421 caaactgcat cacgtcacta tgcacacgtt gactgtcctg gtcacgctga ctacgttaaa
      481 aacatggtta caggtgctgc tcaaatggac ggagctatta tagttgttgc tgcaactgat
      541 ggacctatgc ctcaaactcg tgagcatatc cttttagctc gtcaggtaaa cgttccaaga
      601 attgtagtat tcttgaacaa agttgactta gttgatgacg aagaactttt agaattagtt
      661 gaaatggaag ttcgtgattt attgaatttc tacggttttg aaggtgatga ttgtccagtt
      721 attagaggtt ctgcattagg tgcattaaat ggtgaaccac aatgggaaga caaagttatg
      781 gaattaatgg aagctgttga tacttggatt ccaattcctc cacgtgataa cgaaaaacca
      841 ttcttaatgc ctgttgaaga cgtattctct attacaggtc gtggtacagt tgctactggt
      901 cgtatcgaaa ctggcgttat caatacaggt aacgaagttc atattatcgg tttaggtgaa
      961 gaagctcgca aaactgttgt tacaggagtt gaaatgttcc gtaaaatatt agacaaaggc
     1021 gaagctggtg ataacgttgg tctattactt cgtggtgttg ataaagacga agttaaaaga
     1081 ggtcaagtaa ttgcacaacc aggaacaatt aaaccacata caagatttaa agctgaagtt
     1141 tatatcttga aaaaagaaga aggtggtcgt cacactcctt tccacgataa atatcgtcca
     1201 caattcttct tgagaacatt agatattact ggtgaaattc atttgccaga aggcgttcaa
     1261 atggttatgc ctggtgataa tgtttcaatt gaagttgaat taatttaccc agtagcttgt
     1321 aacgtaggtc ttcgttttgc tatccgtgaa ggtggtagaa cagttggagc aggacaaatt
     1381 actgaaatta ttgaataatt aaaaccaata tgatagatga agcgaggttt tccttgcttc
     1441 ttctttctac gggtgtagct cagttggtag agcactagtc tccaaaacta ggtgtcgggc
     1501 gttcgagtcg ctcctcccgt gcaaacgaga tattaagatg agaaaaatta aagaatattt
     1561 caaagatact tacaacgaac tagttcataa agttacttgg cccaaatggt cagaacttca
     1621 gggcagtgca gttgtggtta tggttgcttc cttaatattt gccctaatta tcttcgtgat
     1681 ggattatagt tttaaaaatc ttctcgaagt tatttacggc atattttatt aaacctaatt
     1741 ttgtcggata tgtcagagat tgaaaaaaaa tggtatgttg ttagagctct tagcggaaaa
     1801 gagaaaaagg taaaagaata ccttgaagca gagattgcta ggcttggact acaagatcaa
     1861 gtttctcagg ttcttatacc gacagaaaaa gtttatcagg tcagaaatgg aaaacctatt
     1921 acaaaggaaa gaaatttttt tccaggttat attttgatac atgctgcttt agacggtgaa
     1981 atagagcaca gtataaaaaa tgtaagcgga gtaattggtt ttttaaaaac ccaagatgga
     2041 cacgcaatac ctttgcgaaa tgatgaggta gctagaatac ttggaaaagt tgacgagcta
     2101 gctgagagtg atgaagaggt aaatatgctc ttttatgttg gagaaccagt aaaggttatc
     2161 gatggtccat ttagcggttt tagtggtgtg atagaagaag tgtatgatga gaagaaaaaa
     2221 gtgaaagtaa tggttaagat ttttggtcgt agaactccac tagaacttcg attcatacaa
     2281 gttgaaaaag agtaatcttt attaagaaaa aaagtttatg gcaaaacaaa ttagtggttt
     2341 tattaaacta caagttaaag gtggtgtcgc aaatccatct ccacccttgg gtcctgcttt
     2401 aggatcaaaa ggtttaaata tcatggagtt ctgcaaacaa tttaatggta gaacccaaga
     2461 tcaagtaggt aaagttttac ctgtgattat cacttattat agtgataaat cttttgattt
     2521 tatcgtaaag actccaccgg ttgcgattca attgttagag caatcaaaag ttaacaaagg
     2581 ctctgcagaa ccaaatagaa ataaagttgg ttcagtaact tgggatcaag ttaaagctat
     2641 tgcagaagct aaaatggttg acttaaatgc tttcacatta gattcagcaa tgagtatggt
     2701 tgcaggcaca gctagaagta tggggatttc tgtaaaagga caatcaccaa ttaaaaaata
     2761 attaaagtac tttttgaaat ggcaaaaatt agcaaaaata gaaaaaacgc tttagctaaa
     2821 cttgaaaaag gaaagaagta cgatcttcaa gaggctgcaa aacttgtaaa agagattact
     2881 tttacaaagt ttgatgcttc tgttgatatt gatgttcgtt taggtgttga ccctcgtaaa
     2941 gccaatcaaa tggttagagg aattacaaca ttacctcacg gaactggtaa agaagttcga
     3001 gttttggtat tatgtactcc agataaagag caagaagcaa aagatgctgg tgctgattat
     3061 gttggattag ataattatat tgaacaactt tcaggaggct ggacagatat cgatattata
     3121 attactatgc ctcaggtaat ggcaaaacta ggaaaactag gtagaatctt aggccctaga
     3181 ggtttaatgc caaacccaaa agctggtaca gtaactatgg agatcggcaa agcagttgaa
     3241 gatgtgaaaa aaggaaagat tgattttaaa gtggataaat acggtataat ccatgcaggt
     3301 attggtaaag tttcatttag tcctgaaaaa atagttgata acgcaactga aataattcag
     3361 acaattataa aacttaaacc aacagcaaca aaaggaactt atgtaagaag tatttacttg
     3421 tctagcacaa tgagtccagg tattgaaatc gatcttaagt caattaatta gtaattaaca
     3481 aattgccagt aatgaaacga gaagataaaa atgcacttat cgaaagtttg aaagatacta
     3541 tcaacgaata taaccacttt tatctaacag atatttcggg gttaaatgcg gcagatactt
     3601 ttgaattaag aaaaaaatgt ttcgaaaaag gcatagaact gattgttgtt aagaatactt
     3661 tttttaagca agctttagaa cgaagcggtc gtgattttca ggaactatat ggtgttttaa
     3721 aagaaaacac tacagtaatg tttactaatg ttgcaaatgt tcctggtaaa ttaattcaag
     3781 aacttaataa aaaaagtgat aaaccgcttt tcaaaggagc ttatgctgaa gaatcgtttt
     3841 atattggtgc agaccagtta agcgctttag caagtcttaa atctaaagaa gaacttattg
     3901 gagatattat tttattgtta caatcaccaa taaaaacagt tcttggacaa cttgaatcag
     3961 gtaaaaattt attaggaggt ttagtaaaaa ccttatcaga aagagaatag aaaatttagt
     4021 ttaaaacaaa ttaaaaacaa attaaaatgg cagatcttaa gaagttagca gaagaattag
     4081 ttaaccttac tgttaaggat gtaaatgaat tagcaaacat cctaaaagaa gaatatggta
     4141 ttgagccagc agctgcagca gttgcagtag caggaccagt tgcaggtggc gctggtgaag
     4201 tagctgaagc tgaaaaaaca gagtttgacg ttattttgaa aagcgcaggc gctcaaaaat
     4261 tacaaattgt taaattagta aaagaattaa caggactagg tcttaaagaa gctaaagccc
     4321 ttgttgatga agctccaaaa ccaattaaag aaggagttcc aaaagacgag gcaaatgcac
     4381 ttaaaactca attagaagag gctggtgcag aagtcgaact taaataaaca acttttgttg
     4441 tattaaatta taggtttaaa gcccttttgg gctttaaacc tttcgttgtt aatgtttagg
     4501 ttcttttaaa taataaaaaa caatgattag taataagaaa caaagaatta atttttctac
     4561 aacaaaaaat cagttagagt atcctgattt tttagaaata caaataaagt catttgagga
     4621 gttttttcaa ctaaactcca ccgcagaaca aagaaaatct gaaggtttgt atcaagtgtt
     4681 tatggataat tttccaataa acgatactag aaataatttt gtattagaat ttttagatta
     4741 tactttagat gcaccacgat acagcataca agaatgtgtt gaacgtggat taacttatag
     4801 cgtgccttta aaagcaaagc taaaactgta ttgtactgat acagaacatg aagattttga
     4861 aacttcggtt caagatgttt ggctaggtgc agtgccatac atgacaccaa gtggaacttt
     4921 tgtgataaat ggagcagaaa gagtaatcgt ttcgcaacta cataggtcgc caggcgtatt
     4981 ctttagtcaa tctattcata ctaatggaac taaattatat tcagctcgta ttatcccatt
     5041 taaaggttct tggattgagt ttgcaacaga tatcaatagt gtaatgtacg catatattga
     5101 tagaaaaaag aaattacctg ttacaacttt acttcgagct atcggttttg aaagtgataa
     5161 agatatttta gaaatttttg acttagccga tgaaataaaa gtgtctaaaa ctgctcttaa
     5221 aagagttgtc ggtcgaagat tagcagcaag agttttaaaa acgtggatag aagatttcgt
     5281 tgatgaagat acagctgaag tagtatcaat agaacgtaac gaagttgtta ttgatagaga
     5341 aatagtccta gaaccacagc atatagattt gattttagaa tcaggaacta aaactattct
     5401 tttacataaa gaaaatcaaa aagtttctga ttatgcaata attttcaaca ctttacaaaa
     5461 agacccatgt aactcggaaa aagaagctgt tgtaagtatt tatcgtcagt tacgtagtag
     5521 cgaaccacca gacgaagcta ctgcacgtga tgttattgat aaattattct tttccgacaa
     5581 acgttacgac ttaggagatg ttggtcgttt tagaatgaac aagaaattaa acttgaatat
     5641 tcctgacgaa atccgcattc ttactaaaga agatataatt gaaataataa aatatttaat
     5701 tgagttagta aactcaaagg cagatgttga tgatatcgac cacttaagta accgtagagt
     5761 aagaacagtt ggagaacaat tagccaatca atttggtgtt ggtttagctc gtatggctcg
     5821 tactattcgc gaaagaatga atgttagaga caatgagatt ttccatccaa ttgatcttat
     5881 caactcaaaa acattatcgt ctgtaataaa ttcatttttt gggacaaatc aattgtctca
     5941 atttatggat caaataaatc cattagcaga aattactcat aaaagacgac tttcagcatt
     6001 aggtccaggt ggactttctc gcgaaagagc cggttttgag gttagagacg ttcactatac
     6061 tcactatgga cgtttgtgtc ctatcgaaac tccagaaggt ccaaatattg gtttgatttc
     6121 ttcactttgt gtttatgcaa aaataaataa actcggattt attgaaactc cttatcgaaa
     6181 agttgttgat ggaaaagtat gtttcgatga tatagtttat ttaagtgcag aagaagaaga
     6241 agaaaaagtt attgctcaag ctaatgctct gattgatgat gacggaaact tcttgtcatc
     6301 aagagtaaaa tcaagagatg aaggtgattt tgtagtaaca gatcctcaac aaattgagtt
     6361 aatggacgtt gcaccaaatc aaatagcgtc aatagcagct tctttaattc catttttaga
     6421 gcatgacgat gctaaccgtg ctttaatggg atctaacatg atgcgtcaag ctgtgccttt
     6481 gttaagagca gaagctccaa ttgtaggaac aggtatagaa gaaatggttg ctcgcgattc
     6541 acgaatttta attactgctg aaaataacgg tgtggttgaa tatgtggacg cagaaaaaat
     6601 tatagttaag tacgatagaa ccgaagaaga agaattttta agttttacag ataattttgt
     6661 tacttataat attccaaaat ttcaaaaaac taaccaaaat acttgtgtaa acctacgtcc
     6721 acttgttaaa aaaggcgata aagttaaaaa aggcgattta ttaaccgaag gttatggaac
     6781 agaaaaagga gaattagctt tgggacgtaa tcttaaagtt gcttttatgc cttggaaagg
     6841 ttacaacttt gaagacgcta ttgtattaaa tgaaaatata gttaaagaag attattttac
     6901 atctatccat attgcagatt atcaattaga agtacgcgaa acaaaacgag gactagaaga
     6961 attaacttca gatattccta atgtaagcga aactgcaact aaagatttag atgagaatgg
     7021 aattattagg gtaggtgcat atgtgaaacc tggagatata attataggta aaattactcc
     7081 aaaaggagaa tctgatcctt ctccagaaga aaaacttttg agagctattt ttggtgaaaa
     7141 agctggtgat gtaaaagatg cttcattaaa agcttcgcca tcaactagtg gagttgtgat
     7201 tgataaaaaa ttgttctcaa aaacaattaa agatcgtaaa cttagaaatc aagataaaat
     7261 tttagttgaa aatcttgata aaaaacatgc agaagaatct gctgctctaa aaagtatgtt
     7321 gattgaaaaa cttttcactc ttgtgaatgg aaaaacatca caaggtgtta aagataattt
     7381 taacaatgat ttaattccaa aaggaacaaa atttactcaa aaattattag cagaattaga
     7441 ttttattaat gtaagtcctg gacgttggac aaccgacaaa aatagaaacg aaactatttc
     7501 aaaacttttg tttaattata tggttgaaca aaagaaaata gatggtgatt atcgtcgtaa
     7561 aaaactcgaa attactattg gagacgaact tcctgctgga ataattcaat tggcaaaagt
     7621 ttatattgca agtaaatgtg ttgttaaagt tggtgataaa tttgctggtc gtcatggtaa
     7681 taaaggtatc ttggcaaaaa ttgtacgtca agaagatatg cctttcttag aagatggtac
     7741 acctgttgat attgttttga atcctttggg cgtgccttct cgtatgaact taggacaaat
     7801 ttatgaaaca attttaggtt gggcaggtag tgttttgggt tgtagatttt caactccaat
     7861 ttttgatggt gctaatatag accaaattaa tgaatatact aaaaaagctg gtttaccaga
     7921 gtacggaagc acttatttgt atgacggtgg aacaggcgat agatttgacc agccagcaac
     7981 ggttggagta atttatatgt taaaattaag acacatggtt gaagataaaa tgcacgcgcg
     8041 ttctattggt ccttattcat taattacaca acaacctctt ggtggtaaag cacaatttgg
     8101 aggtcaacgt tttggagaaa tggaggtttg ggcactcgaa gcatttggtg ctgctaatat
     8161 acttcaagaa attctaactg ttaagtctga tgatgtggtt ggtcgtgcaa aaacttatga
     8221 agcaatagtt aaaggcgaac aaatgccaga accaggaatc ccagaatctc taaatgtgtt
     8281 ggtacatgag ttaagaggtt tgtgtttgag agtagatctt gattaaaaaa taatttaata
     8341 gctgataaaa atatggcttt tagaaaagat aaaaaaataa aaacaaaatt ttcaaaaatt
     8401 actattagtc ttgcttcttc tgaagaaatt caggagatgt ctagtggcga agtaactaaa
     8461 ccagaaacta taaactatcg tacttataaa ccagaaagag atggtttgtt ttgcgaaaga
     8521 atatttggtc cagtaaaaga ttatgaatgt cattgcggta aatacaaaag aattagatac
     8581 aaaggaatag tttgtgatag atgtggtgtt gaagttaccg agaaaaaagt aagacgcgaa
     8641 agaattggac atattaaact tgaagttcca gtagcgcata tttggtactt taagactttg
     8701 ccaaacaaaa taggttattt gctcggacta ccaagtaaaa aactcgattc tgtaatttat
     8761 tacgaacgtt atatcgttgt gcagccagga gtaaaagcta ttgatggtat tcaagaatta
     8821 gactttttaa cagaagatga atatttggaa attttagata gcctgccacg cgaaaatcaa
     8881 cttttagaag atacagaccc aaataaattt atcgcaaaaa tgggtgctga agctatccaa
     8941 gatttgctta aaagaattga tttagatcaa ttgtcatatt ctttgagaga taaagcaagt
     9001 aatgaaactt cacaacaaag aaaaagcgag gcgctaaaac gtttgaatgt tgtagaagca
     9061 tttagacttt ctgctgatag aaataaacct gagtggatga taatcaatgt tatcccagtt
     9121 attccaccag agttaagacc attagtacca ctagatggtg gacgttttgc aacttccgac
     9181 ctaaatgatt tgtatcgtag agttattatc agaaataata ggcttaaacg tttaatcgaa
     9241 attaaagctc cagaagtaat attacgtaac gaaaaacgta tgttacaaga agcagtagat
     9301 tctctttttg ataactcaag aaaatcaaat gctgtaaaaa ctcaagcaaa tagaccttta
     9361 aaatctctta gtgatagctt aaaaggaaaa caaggtcgtt tccgtcaaaa cttattaggt
     9421 aaacgcgttg acttttctgc tcgttcggta attgttgttg gtccagattt aaaaatgcac
     9481 gaatgtggat tacctaaaga aatggcagca gaattataca aaccttttat tattcgtaag
     9541 ttaactgaac gcggaatagt aaaaactgtt aaatctgcta aaaaaattat tgaaagaaaa
     9601 gatccagtta tttgggatat acttgaaaac gttttaaaag gtcatccagt tttgttaaac
     9661 cgtgctccta ctttgcaccg cttaggtatt caggcatttc aaccaaaact tatcgaagga
     9721 aaagcaattc aattgcaccc attagtttgt acagcattta atgctgactt cgacggtgac
     9781 caaatggcag tgcatttacc actcggtaac gcagctattc ttgaagcaca agttttaatg
     9841 ttggcttctc ataatattct aaatccagct aatggagctc ctattactgt tccttctcaa
     9901 gacatggttt taggtttgta ttatctaact aaaggtagaa aaggagtaaa aggagaagga
     9961 tatacatatt attctcctga agaagttatt atcgctaata acgaaggaca attagatctt
    10021 catgctaata taaaagtaag agttgacgat gtaaacgaaa aaggagaagc ggttacaaga
    10081 atgatagaaa ctactacagg tagagtatta tttaaccaaa tcgttcctaa agaatacgga
    10141 tatattaatg aaattcttac aaaaaaatca ttacgcgata ttattggaaa ccttgtagga
    10201 aggtttggaa cagacgtaac ttcaagattc ctagatgata taaaagactt aggattttct
    10261 catgcgttta aaggtggatt gtcgtttaat ttgaatgacg ttattattcc tgaaacaaaa
    10321 caagaattaa ttcaagaagg atatgctgag gttgatgaaa tcatgtttaa ctatgaaaat
    10381 ggttttatta ccgatactga acgctacaat caagtaattg acgtatggac taatataaac
    10441 gctaggttat ctatcgatgt tatgaaaaag ctttctactg atgaccaagg ctttaatgca
    10501 gtttatatga tgcttgactc tggagctaga ggttctaaag agcaaattag ccaattgtgc
    10561 ggaatgaggg gacttatggc aaaaccacaa aaatcaggtg ttgcaactag cgaaattatt
    10621 gaaaacccaa ttttagcaaa ctttaaagaa ggtttgtcaa ttcttgaata tttcgtttct
    10681 actcacggtg ctcgtaaagg tcttgcagat acagctttaa aaactgccga tgcgggatat
    10741 ttaacaagaa gattagttga cgttgcacaa gacgttatta ttaccgaaga agattgtgga
    10801 acattaagag gattggtagc tgttgaacta aagaaaaata atgaagaagt aattgaatct
    10861 ctttatgaac gtatcttagg tcgtactagt gttcatgata tttaccatcc acaaactgaa
    10921 gaattaatag ttgctgcagg tgaagaaatt actgaaagaa ttgcaaaaat tattgacgaa
    10981 tctccaattg aaaaagttga aattcgttcg gtattaactt gtgaatctcg aaaaggagtt
    11041 tgtgcaaaat gttatggacg taatttggca actcacagaa tggttcaaaa aggagaagct
    11101 gtaggagtta ttgcagcaca atctatcgga gagcctggta ctcagttaac tctacgtaca
    11161 ttccacatgg gaggggttgc tggtaaaatt gcgggagaat catttataga cgcaaaatat
    11221 gacggtatcc tagaaattga tgagttaaga acagttgaac ataaagaaaa agatggttct
    11281 cttgttgata ttgtagtaac tcatatggct gaaatgaaaa ttttagaccc aaatacaaaa
    11341 atacctctag taaacaaacc tataccttat ggttctaaat tgtactttaa gcaaggtaat
    11401 agtgttaaaa agggagatag aatctgtgat tgggacttat ataatatcgt tattgttagt
    11461 gaagtttcgg gaaaactagt gtttgaagac gttatcgaag gtgtcaacta taaagatgaa
    11521 ttgataattc aaactggttt aacagaaaaa attataatcg aaactaagga taaaactaaa
    11581 attccagttg ccaaaattgt tgataaaaat aacgaagtat tagcaactta taatttacct
    11641 gtaggtgctc gtttgcaaat tgaagaaaac gaaaaagttg aatctggaga tatcttgatt
    11701 aaaatgccta gaattgtcgg aaaaggtgga gatattaccg gtggtttgcc tagagtaaca
    11761 gagttgtttg aggctagaaa tccatctaat cctgctgtag tttcagaaat tgacggggaa
    11821 gtatcttttg gtaaaattaa aagaggaaat agagaaattg aaattaccgc aaaagacggt
    11881 aaggttaata agtatttagt acctctttca aaacaaattt tagttcaacc aaacgactat
    11941 gttaaagctg gtacaccact tatcgatgga gcaattactc ctagcgatat tttagcaatt
    12001 aaaggtccta ccgaagttca agaatattta gtgaatgaaa tccaagacgt atatcgttta
    12061 caaggtgtga aaattaatga caaacacttt gaagtgattg tacgccaaat gatgagaaaa
    12121 gttgaagttg ttgaacctgg tgatactcaa ttccttgaaa aacaactagt tgacaaatgg
    12181 gagtttagag aagaaaatga cgctattttc gataagaaaa ttgttgttga cccaggagac
    12241 tctacaaatt tctctaaagg acaaatgatt tcttatcgta ggttgagaga tgaaaactca
    12301 atattaaaac gtaaagacat gaaactagtt gaagttagag atgctttgcc agctacttct
    12361 agtcaagtgt tacaaggaat tactagagca gctcttcaaa caaatagctt tatgtcagca
    12421 gcttctttcc aagaaacaac taaagttcta aacgaagcag caatacgtgc aaaagttgac
    12481 gaacttgaag gattgaaaga aaacgttatt gttggacata aaattcctgg aggaaccgga
    12541 caaaaagaat ttgaaaatat tagagttaca tctaagatga ttgcagaaca atacttaaat
    12601 caagatgaaa taagtattga agaagaaaaa tttgaagaaa ctgaaattta aaaattatgg
    12661 acgaaaaaaa gcaaaatcaa ataaatattg aactaagcga agaggttgga caaggtgttt
    12721 attctaatct tgcagtaata acacactctt catcggagtt tgtgctggat tttgttagaa
    12781 ttatgccagg aatgcctaag gctaaagtac aatctagaat aattcttact ccagaacacg
    12841 caaaaagatt attaggggct ttgcaagaaa atgttgttaa atttgaaagc attaatggcg
    12901 aaataaaaat tagtaacaat aaaccaggaa cacctattcc aatgaatttt ggaggaccaa
    12961 cagctcaagc ataagtaagt gatattaaat aattaaactt agagtgatct tggggaaaat
    13021 taaattttag ttttccccaa tttttatatt attaagtctg ctttacaata aattttatgt
    13081 tatctttgct tattagattt tataggtaat gaaatattta gttgagataa gaagaaaaag
    13141 aaagaatgat ggtattgcta ttattttgtt tttactttta ttctcattct catcatgttt
    13201 taaaccacaa acatttccca aagaaccaaa tattgaattt attgatttta tagttaaaga
    13261 tacttttgat attttgcaaa atcgtgtatt aaatggcaca ttacattttt attttgttga
    13321 tggagatggc gatataggtt atgatacaac tagccccaga caaaacacaa tctttttaca
    13381 aaaatataaa attgaaaata agcaagaagt tttgattaat atgcaagtac cattagagta
    13441 ctttgtgcct aagtttaaaa gaaacattca agacgaagct ttgcaaggcg aaatgtttgt
    13501 taaagattta aacgaaacct ttccttttga aaacgacaca ataatgtata aattttacat
    13561 aaaagacaga gctggcaata aaagcaatgt agcatctaca gggtacatta ttgtgaataa
    13621 ctatttacaa taacaaaagt tataggtttg ttatcttatt aataatttct aatttagaga
    13681 aaaataaaaa ccaaacatga ttgatcaagt aaccgttcag aaaattattg atgcagctga
    13741 gattacaagc cttattcaag aatttgtatc tttaaaaaga agaggagcaa attatttagg
    13801 tttgtgtcct tttcataatg aaaaaactcc atcgtttaca gtaagtccat caaaaggaat
    13861 ttttaaatgt tttggctgtg gaaaggcagg taatgccgta agttttatta tggctcacga
    13921 acaactttct tatcccgaag ctttaagata tttagcaaaa aaatacaata ttcatataga
    13981 agaaaaagaa ttgaccgaag aagatattcg tgccaataca gaaagagaaa gtatgaatat
    14041 tgtaaacgaa gctgcttgta aatattttga aaacacatta tttaattcag atgagggaat
    14101 atcaataggt ttaagttatt ttaaagaaag aggttttaat gagcaagtta taaaagaatt
    14161 tagattgggt tatagtcttg agcaaaaaaa tgcctttaca gaatgggctt taaaaaacgg
    14221 atataaaaaa gaatttttag agtcaacagg actaagcttt tttaaagaaa attactattt
    14281 cgataggttt gcagcaaggg taatgttccc aattttaagt atcgcaggac aagtagtagg
    14341 ctttggtgca agaactttga gcaatgataa aaaaacagca aaatatctaa attcaccaga
    14401 atctttagtt tataataaaa gtaaagttgt atatggttta tttcatgcaa aaaatgaaat
    14461 aattaagcaa aataaaaaat gctatttggt agaaggttat atggatgtta tatccttgtt
    14521 tatgactgga ataaaaaatg ttgtttcttc atcaggaacc tcattaaccg aagaccaaat
    14581 acgacaaata cataggtttg cagatgatat taccattctt tatgacggag acttagcagg
    14641 aataaaagcc tccctaagag ccatagatat gattttagag caaggtttaa atgttagaat
    14701 tgtacttttc ccagaaggag aagatcccga ttcttacgct aaaaaacatg gaagctctaa
    14761 aactattgaa tatatcgaaa aaaatgaaac agattttatt tcatttaagg caaatttact
    14821 gtttgatgag gctaaaaatg acccaataaa acgagcttct ttaattacag atattgttag
    14881 gtctattgca attatcaacg acaacattaa gcaatctatt tatattcaag agtgtagcaa
    14941 gattttagat gttgaccaaa gtgttttgta tgccgaagtt ttaaagatta gaagatcaaa
    15001 aatcgaaagc attaaaaaac aagaatatag atattctcaa ccagtaaagc ttacacctag
    15061 tttacccgat tcgtttgagg gtatttattt cgaatcacaa gaaaaagaaa ttattagatt
    15121 attgttgtta tatggcaata atatcataga cataaaagat ggtgcaaccg aaacagaatt
    15181 agaaataagt gttgctcaat ttattataaa cgagcttaga agcgaaggat tggaatttaa
    15241 aaatgtaata aataaaaaaa tatttctttt tgctgaagaa ttgataaaca atgatgaacc
    15301 tataaaaata gaattattta ctcataattt agaccctgaa attagtcagg tttctgtaaa
    15361 tttatgttcg tcaaaatatg aacttagtcc aatatgggaa aaaagaggct cgccaattct
    15421 taaagaagaa aagcgattgc aagaaatatt agaagatgtt tttataaaat ataaattaaa
    15481 aattgctaaa gagttcgaaa ctaatgtgaa aaataaaata aaattactta cggaaagcaa
    15541 aaatgacgaa gaactccaaa gcacattaga agattttaaa aaaataaaag gcattttaaa
    15601 agatttatct aatatgcagg ggagggtgat tttgtagtta ttcctattcc aactttactt
    15661 ttttattata ttctttatcc cactactctt cataaaagct aagttttctt attaatgtat
    15721 cataacccat agattttagg tttgtagcag cttcaaccgc cattttgtat gaagtgtatt
    15781 cgccaacata atatctatac caaccgtcac tagaaacatg catttttaca ccaaccaatt
    15841 cagagaaggt ttcaagtgct ttttgatttt tcaaagcata tatttgaaca gtatattttt
    15901 tatttgaagg agttttagaa atatcaatat aatcgccttc ttcaattact attgggagaa
    15961 tttctaatat tgttttatta ccttgaacta gcactttaaa ttctgctctt ctattgtatt
    16021 ttctaccagt cggattatca cgaccatctg gagtttgatt ggcagctata ggagtgtgtt
    16081 taccatattt tgcagttata atgttttcag cattattacc ttttgcaact aaataatcct
    16141 ttactgcttt tgaacgatga taagaaagta aataattata ctcgctagta cctaaccagt
    16201 cggtatgtcc gccgatttca attttggcag taggattatc atttaagtaa tttgctagta
    16261 aatctaaatt atcaaaatat tcaggtttta tattgtattt atcaaaatca aataaaatat
    16321 tttcaacttt aagtattgct ttatctacta taacttcttc tttttcttcg atagctagca
    16381 attttttgtt ttcaacaatt tctaatccat aaatattggt tgagttatta tcaaatacac
    16441 catcgctgta tatatcgttt ttagctatat tgtttttgct taaatattca aaaatttctt
    16501 ttgaacgttt ttcatcatat aatggatttg cgtcttgttt tggtgtaaca atatttattg
    16561 caagtttttc gtttttagct tcaaattcta catacttttc aatcacttta tcaagtaaac
    16621 ttatagcatc atcatttaag ttattatcgt tttcatcaaa atatacatat gcatgattat
    16681 aaaacgaact taaaataata gggtctaatt ctattgggtt atatagtttt gtttgttgtg
    16741 ttaattcatc tatagttgga gtgtaatcaa tagaacgaat ggtatgtccg tgtatttgat
    16801 aaataatttc ataagtgttg tctggaatca tagttaatac atattttgaa ttattttgat
    16861 ttaaacgaga tacttttggt tcttcattag aatctttatt ttttaaagtt atggttacat
    16921 tttcagggag attattattt ttatcagcaa taattccttc ataaataatt atattgtttt
    16981 tatgaacatc ttcttgtaat atttcgtaca aatctaagtc tccataaccg tcatcagctt
    17041 ttattgttgc tatatagcct ttttgtccat caagatttac aacataagaa acatcatcgt
    17101 ctggcgtatt tattgggtat ccaatattta caggttctgt aaatttgcca ttcctgctca
    17161 taatagatga aaacacgtca taacctccca tagaattgtg tcctttggaa ctaaaataca
    17221 aagtaatgcc gtcagggtga ataaaaggag attcttcatc atattctgtg ttaatttgtt
    17281 tgggtaaagg caaagcttcg ctccattttc cgtttggtaa cttcctcata acatatatat
    17341 ctaaaccacc aaatcctcct tttctgttac tagtaaaaaa tatagcattt ccatctgggc
    17401 taattgtagc atgagtttcc ttaaatcttg agttcacatt ttcttttagc aaagttggag
    17461 ttgaccaatt atacatatca tcactttctg ttataaaaat actgccagag ttattttctc
    17521 ccgacctgta aaagaataac tgtttagaat ctatagacaa tccaattgca gcttcattgt
    17581 tatcaattgt attaacaggt tctcctaatc taataggagt ttcccactta ttattaactt
    17641 ttttagaaat aaaaatatct tcatcttgat ttttcatttc ttgtccttcg cgtggtcgcc
    17701 tagatgtaaa gattaagtaa ttttcatata aatcaacaat aggggagtga tcggtaaatt
    17761 cagaattgat aacctctcct aagttgatta attcagcttt tattgggttt gcaacattat
    17821 aaattgcgtt ttctgtaatt tcaatttctc tttttattat ttttaataga gccttgtctt
    17881 ctgtttttga aggactaagc atgcctttaa gcttatcaaa gctatcgaga ctttcctgaa
    17941 acatgtaatt taccctataa gcaattccta aatataaata agattctaaa ggtgtatttt
    18001 tttctttatg gttattttct ttataatttg gcgaagtgta gtttgatgct ttttttaaat
    18061 actcaagagc ctgtgttttg taaagtcttg tatttaagta acatacgcct attttatacc
    18121 aaatgtttgg gttgtcagga cttatgttat ctaattttaa caacaagtct aaggcttcat
    18181 cgtaattacc ttcgtacata aatgcttcag catcataaaa ataattaatg ctagttctat
    18241 caggttttaa ttcttgagtg tgtgctagaa cgttaacaag cattagtaaa gaaagaaata
    18301 ctgtcttttg catattcata agaaaattaa tttaaatttt taataaataa ttttagggta
    18361 ttataatcga actcacattc ttctattaat tgttgttggc ttccatgaga aataaatctg
    18421 tcaggaatac caaagcgata tatttttgaa ttaaatttgt tgtcaaccat aaattcagat
    18481 attgcagagc caaatccacc aataatactg ttgtcttcaa tagttatgat atgtttataa
    18541 ttgttaaata tttctgttaa tagtttattg tcaagaggtt ttacaaaaat catatcaaaa
    18601 actgcaatgt ttaaattttc ttcttctaaa tcgttagcta gctttattgc agtatttcca
    18661 ggagtgccaa tactaagtat tgcaatatct tttccatctt taattttttc tccttttcca
    18721 atttcaattt ctttaaattc aattttagat tttgcttgtg tagatttacc acgtggatac
    18781 ctaattacag atggaaaatt atgtttttct aattgtaagg taaacattaa atttctaagt
    18841 tgaaattcat ctctaggagc tgcaataact aagtttggga cacaacgcat atatgataag
    18901 tcgaaactgc cgtggtgggt aggtccatct tgtccaacca agccagctct gtctaaacaa
    18961 aatattactt ttaaattttg taaagccaca tcgtgaatta cttggtcgta tgctctttgc
    19021 ataaacgaac tataaatatt gcaaaaaggt agcattcctt ttattgctaa gccagcagca
    19081 aaagtaacag catgttgttc tgaaatacca acatcaaaac ttcgttcagg aaattcttca
    19141 aacattttgc acaaagaaga accacttatc atagctggag taattgcaca aatttttggg
    19201 tttgcttttg ctaattcaat aatagcatct ccaaaaatat cttgaaattt ttgaggttcg
    19261 ttttcgcaat gactagtgtc ggtaagtttg cctgttgtaa tgtcaaattt accgggtgca
    19321 gcatgccata atgtttgatt ttcttcagca gccttaaatc ctttaccttt tttagtgata
    19381 atatgcaata gctttggtcc tggaatattt ttgatgtctt caaatagttt tactaataaa
    19441 acaacatcat gtccatcaac aggtccaaaa tatctaatgt ttaaagcttc aaataaattg
    19501 ctgtttttta aaacaataga ttttatcgca ttatcaattt tttgtgaaaa agactgaaca
    19561 tttaaaccca aacgattaag cctgcccaag aaataccaaa catcgtcttt aactttatta
    19621 taagttttgg aagtagcaat atctgttaaa tattctttga gtgccccacg gttttcgtca
    19681 attgcaattt tattatcgtt taggacaatt aaaacatttt cgccagacgc acctaggtta
    19741 tttaagcctt caaaagcaat tcctccagtt aaagaaccat cgccaattat tgcaacagtg
    19801 tttatttttt cgccttgtaa atttgctgct tttgcaattc ctaacgcagc cgaaatgctt
    19861 gtagatgcat gtcctacgcc gaaattgtca tattcgcttt ctttgggact tggaaatcca
    19921 ctaatgccac cataagtcct gtttgtgtga aataaatctc ttcttccagt taaaatttta
    19981 tgtccataag cttgatgacc aacgtcccaa accaattgat caacaggtgt gttgtatgcg
    20041 taatgcaatg caactgttaa ttcaactact cctaaacttg ctccaaaatg accaggattt
    20101 tttgatgctg attcaatgat aaaatttctt aactcttcac aaacagttgg taattcactt
    20161 aagtcgattt tttttaaatc atcaggcgaa ttaatgtctt tcaaaagttg atatttttta
    20221 tcatcaatca tagttaatgc aaaagtaaat aaataaaatt agaaaacata tagtaagtag
    20281 ttgatagttt attaaattca tgctgttgaa aagtagaaat ttttaattaa aactatattt
    20341 taatgataaa acaccaagtt tagcagggga ttcttttttt aaaaaatggt gaaagccaag
    20401 cgatatgtct aaactatgtt ttccaaaact atatttatca cctataaaaa gtctaatttt
    20461 actaaaatat ggtaatctaa aaattttatg cagtctgaaa atttcagctg aaaaataaaa
    20521 tttattagct tgtttgtgat agtattcaag ttttatccta ttcctaaact taatttcttg
    20581 caagttatca atttctcggc ttctaaagca attcaaatat tcattttgat aacgcaatct
    20641 gtaaccaagt ctaataacag aactaagttt tatctgatat gaaaagtcaa gattatatct
    20701 taaatcgtgt tgaaaattgt ttaaataatc catgtcgtaa gtgtatcttc cccctgcggc
    20761 tatcgaaaaa tttttattta ttttataaga aaattcaata ttggagatta tatcatcaat
    20821 ttttgtgaaa tttttaaaca atcttaattc ttgttcaaat tctagtttga aacctttata
    20881 taactttttc tcgaaacctg ctgtcaacca tgagccaata tcatttgtaa cagccacttc
    20941 ttgagcattt gtagtactaa caacaagact tagagctatg caggtaaaaa atattttaga
    21001 atgttttatc ataaatttct tttaaattcg gaacaaataa ttgcacttgc aacagataca
    21061 tttaaagatt cggcatgtat atctaattca gaaaaacttg gaataaaaag ttttttgtca
    21121 accatagctt ctaaattttt atttatgcct tttccttcgt tgcccataat aataaatcct
    21181 gcttttggca acgatgattt gtaaatattt tcgccattta aaaaagttcc aaacacatgg
    21241 tttttggtat tgtttttaaa tttaacaatt tcttcagcaa tattggcata aataacatta
    21301 acccttaaaa aagctcccat tgtagcttgt aaagtttttg gagagtataa atctgcagaa
    21361 ttgagagaac aaattaaatt ttttattcca aaccaatcgc aaatacgaat aattgttccc
    21421 aaatttcccg ggtcttgaat ttcatctaaa gctagatata aatcttcatt tttatataaa
    21481 atattacgag attccggcat tgaaactata gccaacacat tagaaggagt aacaaaactg
    21541 cttatctttt tcatttcttc aggtttggct tcaaaaacta aatcctgaaa tttttctaag
    21601 ttatattcat ttttttctaa gtattctggc gaaccacaaa ttaattttat atctaaattt
    21661 gattttaaaa cttcgctcac aattttttca ccttcaacaa caaaaagttt ttccttaatt
    21721 ctatactttt taagttttaa agaattaata aatgatattt tcgacttaga aacttgtttc
    21781 atacaaaaca aaaatataaa aaaaatagaa tattatcatt gtttcatatt tatttttcag
    21841 ttttggcgta gtcaaaggtt ttttattgag attagtgaag ttgattatag agtgttttta
    21901 acttcttttt ttctcaaaaa tatcaatgtt tttaattact tcgttaggat taacggtatt
    21961 gttttcaatt tcaaatctta ttgctgtgat aaatttatgc cagcctgtgt ttcctgccgc
    22021 accaggattt aggtgtaaaa gtttcagatt attatcataa attactttta aaatatggga
    22081 atgaccagca ataaaaatat cgggttttat atctaatagc aagtttttaa tgttaggagc
    22141 atatttccca ggatagcctc caatatgtgt tattagcact ttttgtcctt cgcaaacaaa
    22201 atttaaaaat tctttagttc tagctctgat tttagtgcca tcaatatttc cccaaacggc
    22261 ttttattggt ttaaattttt ctaaatcatc taaaatttgt tcagaaccaa tatcgccagc
    22321 atgccaaatt tcatcacatt ctgcaaaaaa atctaaaatt tctttgtcta aatatccatg
    22381 agtatctgaa agaagaccaa ttttcataaa aatattttta aaaatcctac caattggtag
    22441 gtgtgttggg caaaaataag aaattcctgt tttattttga tagcaaagtt gcgtatttaa
    22501 tgtaaaaata tcaaaatcac ataaactttt taaaaatgtt tatgtcgtat ttattagatt
    22561 ataatttttg ctaactcgct tatatagcac atagtacagt aaaaaaggaa tgtctatttt
    22621 aattaataaa accatgttgt taacataatg ttaataaatt attgctagaa tttgaatatt
    22681 atttattatt tttggataat tattatcatt aagtttaatt aaaatactaa aaattatgga
    22741 agcaaatttt acagtaaaag aagctattat tgaatcaata aagcttggtt tgaaaaattt
    22801 tttatcgtta ataggtgcgg taattctttg gggattaacc atttggattc cttatctaaa
    22861 tgttggaact acaataggta tgattacttt gcctgcaaaa atgagtagag gagctataat
    22921 ttctcctaca gaaatttttg ctagtaagta tcgcaagtac atgggagagt tcttcctcgt
    22981 tacaggttta aagtctatta taatgttccc agctttttta tttatgtatt ttccaggttt
    23041 agttcttcaa tatgctttct ctttcgcaac tttatttgtt gttgataaag gaataaatcc
    23101 tagcgaagca ataagtctta gcaacaaatg tacttatgga tataaatgga aaatgttttt
    23161 tactcattta gtactttttg tagttatttg gattgtattt ttgattttag gctttataac
    23221 tccaatcttg ggtgttatcg cttttgtttt gtttatgcct attttattgg gagctacagc
    23281 atttttctac ggtaaattaa ctgctcatgt tccttcagca gacaatccag aaatagtaga
    23341 aaattaatat attaagtaaa actaaaaaac taaaattaaa aagcgactta tttgtcgctt
    23401 tttttgttta gagaaatttc aaaa
//
LOCUS       DAOPNX010000035        23350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_2742_length_23350_cov_6.018674, whole genome shotgun sequence.
ACCESSION   DAOPNX010000035 DAOPNX010000000
VERSION     DAOPNX010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23350
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2742_length_23350_cov_6.018674"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PKN53_05400"
     CDS             <1..546
                     /locus_tag="PKN53_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOD88420.1"
                     /translation="KISYRETTGGIVVIAKALSTNLTDIKVNDKSLFIILESVEKPGN
                     LGAIARIADAASVDAIIVCDQLSDAYNPNAIRASLGCVFSVKIVNSETDTVLKWLKEN
                     NITSYAAELQATEFYHNCDFSKSSAIVFGTEAFGLSQKWIDSADKRIKIPMLGSIDSL
                     NVANSVAIITFEARRQLNFLV"
     gene            588..1043
                     /locus_tag="PKN53_05405"
     CDS             588..1043
                     /locus_tag="PKN53_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88421.1"
                     /translation="MKNFKIFYLLNVLIFMTFCSNQLFSQTKAFVEITAKTKQSYLDR
                     GYTLVDFKSDSIIDTKPLVSSDIVLNYKTYYIVHVQVDACAYCEYNLYFVDTENVMTE
                     LEPEIVVENNLKQAIYRFDNEENSKGKYVVLLNSKLPYYANIFVFKKKF"
     gene            complement(1040..3187)
                     /locus_tag="PKN53_05410"
     CDS             complement(1040..3187)
                     /locus_tag="PKN53_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOD88422.1"
                     /translation="MNLNKIKMLRYFIVFCFLLFTLNLVFAQKNNQNEISITYRPGQI
                     LKLAKTAQKEGDIFSAIDYYKTYEELNPGRPKVQAALGELYLKEKNYREAQKYLFDSY
                     KLDPKKNLKALFYYAECLKTLGEYDEAKKQFNSFINQAKKNKDLTEEIKISRNHLKGI
                     DSTELWQNKPNNTIVKLLDKEINKPHIDFSPIPFEDNKLIWGSLAEEEVKYYDPNKDE
                     LPVRKFYIAKRDGEDWKNLGEFDETINGSNVNTGNGAFSADKKRFYFTRCAKDFNNKV
                     VCKIFVSNYEDNVWQDPQELPETINLPGSTNTMPTVGVSRLNTDVLYFVSDRENGQGG
                     LDIWFTYYDSKRKEWKEPKNCGKRINTPANELTPYYNIETKTLYFSSDGHPGMGGLDI
                     FKAFGDGKNFENALNLRQPINSSFDDLYFILENNGQRGFFTSNRDGGYSLRHANCCDD
                     VYEFIDKDYINIAVTGKIFGITDSAFFKTIEKEYQQEMALSLNVLDRSDDIELLYNYQ
                     VSLFMIDKNSGKELFVKNDFTTPEYYFFNLEQGFDYFISVKDYNKKEKKLPFTTKNIT
                     RSDTIVLDAIFVNTLPEQSFIVKNIYYEFAKSELTTEAKKTIDNTIFKILQAYPDIII
                     EISSHTDSIDTEEFNMKLSQARAQSVVDYLINKGIESERLVAKGYGKNFPIAPNSNPD
                     GSDNPEGRQKNRRTEFKVIGQLNNEEEVIYEDY"
     gene            complement(3191..4213)
                     /locus_tag="PKN53_05415"
     CDS             complement(3191..4213)
                     /locus_tag="PKN53_05415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOD88423.1"
                     /translation="MKKIFIIISTVTLIFGYFCTKAYCQDIHFSQFTASPLSLNPAET
                     GFFDENWRFTNNYRTQWAAIGTPYRTISVGFDKSMHTKKGKNFGLGAFFINDHSGSSN
                     LLVNKIFVNGSYILSLNETNVLSFGAQIGFVTKNFTLNGLSLPSQFNDNTGLFDSNLP
                     NNITQTVENIQYLDINLGIKYKVTIKNLTPHFGLSFFHVNNPKETFVNTDNKLPMRVA
                     LNSGVTLPIKDNIDIVPNILVMLHKKTNDFVLGGIANFKLKPHKQISNIFAGIYERNS
                     LNNADAIIFTAGLGIYGFDLGFSYDVNISALKAATKNRGAFEISIIYKHLTQKVKSVT
                     IPCDRY"
     gene            4397..8794
                     /locus_tag="PKN53_05420"
     CDS             4397..8794
                     /locus_tag="PKN53_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOD88424.1"
                     /translation="MKRIYILFLLIFSVTRLFSQPTADFTSSGNNLCAPAIITFTNTS
                     TGCSGTPTFYWTSGTSDVSSLENPTFSYTVGGTYTVSLKITCDGFEDTKTMTIVVYDP
                     PEANFSNVVQKGCVPFDVNFIDMSLTGDAAINSWVWHFGDGTSSTEQHPSHTYASSGS
                     FNVTLFVKDENNCSSQVTHNNLIRVANLPTVSFVADNPTLCTSPHTVNFSSSVTTSFG
                     LTATYLWNFGDGGTSIENNPSHTYTSGIYDVSLTVTDEYGCENTFSKPEYVRIEPAVP
                     QYSVLEGDTVCKGMQTHFLNETGYSCRWDFGDGGTSMQNTPIHIYQQTGNVNVTFTVD
                     PGGQCEASTEFTLFVETITASFTTNPTNLYSCSIPFEVEFTNTSSANATSFYYVFQDG
                     TSSIQPNPSHTYSSGGIFQPTLTVTSNAGCVHTFMGPIINISPADASFTADEVEGCEP
                     LTITFTYSGDPEAITNFNWNFGNGNTNSDGSAIESSVYNAGEYTVVLTVTDGQGCTGE
                     SSLELNIGAVYEPNINVFDNDPDHTPLLSHNICAQDTVSLWVLEWDNEDYEFTWWIDS
                     TSNKDVSNEYTDHAFDQDTGWVHLHMITLYNGCRDTIYWDSLYISGPIIDGISKSYDC
                     NNPRNYEFTLNETIADTWDWKIYYIHEGTMTIVEETLSSTDHNYSYTFPPSPDSFWVK
                     VTAHSDTTSCEYVDSIQIKIATPSASFSIPVYNNCTGVDISFDASASQDASEYYWDFG
                     DGNNSGWLSEPIFIYQYMTVGVFDVVLTVRDENGCEDTDTHPIHIIGPEIHTTISETY
                     GCDQLLVSFSDFSTASEPIVSVEWNFGDGSTMIGTDVTHLYSTPGVYSVTVNVTTLSN
                     CGLGITYQDTIVVESVDASFNALPVVACVNQDISFQALENNISYIYTWNFGDTNPAIS
                     GHSPSINYSYENGGYYDVHLKVENGRGCEDELLLEGFIKIEQAFANFSLTEDHFSCYP
                     ATPIIIQNSSVLPADTELYYQWILGSDNDTITVEEPTYVYTVPGNFDIVLNLSTPAGC
                     TDTHSLPLIIEGPYAQMNISDTIACVGQEIEFSLTEQENVETIQWVVGGGDSYTVESF
                     THSYSMVPPSGYYTVNLVIKSGDCQPPSFVQNIYIFDVTADFSITDTENNEILGGLCS
                     PFEAMLVSNSINDDFRYWFVDNHPIGSGLSTETHNFVNNQSYDTSGEITLIVEDIHGC
                     LDTIKKSIDVYALPVIEITQDTIICKGDEIKLYSAGGVSYLWSPDVNISDVNSQSPSV
                     KPEEDIIYYLEVRNSHDCLSIDSVSILVIQDFLVNFTPVYDSIIIGDTTRIILEATQD
                     SLIYTWTPSTNISCTNCPEPEFYPLEDTRYRLVVEDSTGCFKYHYLLDIFVIEEYTLD
                     VPGAFTPGSGDKNAVIYARGFGIKKLLQFRIYNRWGEEVFYTDDINQGWNGYYKGKLQ
                     SIDNYSYFIEAEMFNGSIQTKKGHIMLIR"
     gene            8882..9844
                     /gene="rfaD"
                     /locus_tag="PKN53_05425"
     CDS             8882..9844
                     /gene="rfaD"
                     /locus_tag="PKN53_05425"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR02197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HOD88425.1"
                     /translation="MIIITGAAGFIASCLVTELNNRGYKDLVLVDDFSKSEKKNNYSD
                     KTFTKLIDRNEFDYWLDRNHKLVQLIFHIGARTDTTEFNFEILEKLNLEYSKLIWRKS
                     YEYGLPLIYASSAATYGAGEFGFDDNTSPELLKPLNPYGISKNEFDKWALNNEKKPYF
                     WAGFKFFNVYGPNEYHKGRMASVVYHAYNQIKETKKLKLFKSHNPKFEDGMQLRDFIY
                     VKDVCSVLIHFMETRKNSGIYNLGTGKARSFLDLGKACFNALSIEPNIEFVDIPLDIR
                     DKYQYFTQANVEKLRSVGYDKPFYSIEEGVNDYISNYLKEEKYF"
     gene            9848..10963
                     /gene="meaB"
                     /locus_tag="PKN53_05430"
     CDS             9848..10963
                     /gene="meaB"
                     /locus_tag="PKN53_05430"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOD88426.1"
                     /translation="MSNKKKYIGEVEEAKADNSALKVNQGIEQPPQVNPNFRKFKRIK
                     KDLTVSDYVEGIVAGDVNILSQAITLVESRLAKHQEIAQEIIKECLHLDKKSIRIGIT
                     GVPGAGKSTFIEALGMEIVNRGHKLAVLAVDPSSERSGGSILGDKTRMEELSAQKNAF
                     IRPSPSAGSLGGVARKTRETIVLCEAAGYDTIFIETVGVGQSETAVHSMVDFFLLLML
                     SGAGDELQGIKRGIMEMADAIAITKADGNNVQKAKLARVQYQNALHLFPQTEAKWEPK
                     VLTCSSIEKKGISEIWETIKEYETHTRNNGYFENNRLKQSKYWLFEAINSALKERFYD
                     DKLVKTELRKLEVKIEKNEISSFVAATQLLDIYFQKK"
     gene            10960..11463
                     /locus_tag="PKN53_05435"
     CDS             10960..11463
                     /locus_tag="PKN53_05435"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOD88427.1"
                     /translation="MIGQKIVYETLEKLNISFEYYEAPKDFSNEEDISFWNRIGATRC
                     KNLFMRNHKGNRHFLLISDYYRDINIKVLEDKFKKGKISFASQQRIDKHIKGVIGAIS
                     VFSLLNDEANHVEVFIDEGLKTKQKLTFLPNELNALIAISFEDFIKILDYSGNKYEFM
                     NLAASVE"
     gene            complement(11464..12435)
                     /locus_tag="PKN53_05440"
     CDS             complement(11464..12435)
                     /locus_tag="PKN53_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOD88428.1"
                     /translation="MKHLFYIFLSLIFIPNLIFSQNDKNARIIIDTVNSKGFDIILFE
                     NNTWEYINQDSVLAVIKFNDSIKLYNDIYENRLLELDSLTVFSENWDTINIFAFGKAD
                     YKRTLDTCAISLLNNGEKFYMPYIGTVISKFGWRRGYHHNGIDIRLKTGDTIVSAFDG
                     IVRYAGWNRGGYGYLVIIRHYNGIETYYAHLSKLLCQRNQEVKAGDTIARGGNTGKSY
                     GPHLHFETRFKDNPFNPELIIDFENNKLKSDTLVLMPEEFAYLKSRRRTSGGVSGVLE
                     SNYHIVYSGDTLWAISRKYNISISQICSMNGISEDSKLQIGQKIRVK"
     gene            12665..12738
                     /locus_tag="PKN53_05445"
     tRNA            12665..12738
                     /locus_tag="PKN53_05445"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12699..12701,aa:Pro,seq:ggg)
     gene            12887..13216
                     /locus_tag="PKN53_05450"
     CDS             12887..13216
                     /locus_tag="PKN53_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014798146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOD88429.1"
                     /translation="MKMTSFGKFIKAEREKRGWSQTELGALIKINTPLVSRIENDKKL
                     MSFDKVELLANAFEMDYQELKDLYFADKTAKSAYEYKCSKNVFAVAEKQYEYIEATNI
                     VQGQLKF"
     gene            13222..14004
                     /locus_tag="PKN53_05455"
     CDS             13222..14004
                     /locus_tag="PKN53_05455"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOD88430.1"
                     /translation="MDNKKLIQTLGFVPKENSSGIFQKKYSKADYYVVEIDFEKENIN
                     YGNKIKGESKTTQNFLQAENWVVLECVDRLLGKGYQPQNITLEKTWKTGHGTSGRLDI
                     LVTKDDGSAYLMIECKTWGTEYEKELKNLEKNGGQLFTYFQQDKNADVLMLYTSKIDS
                     KGVEYKNTIVKIEEDYKQTGNVKDFYERWNKLPKTNGIFDSWVRPYEFQSKALTRRDL
                     KALKQKDSSFIFNRFLEILRHNVVSDKPNAFNKIFTLYTISV"
     gene            14054..16909
                     /locus_tag="PKN53_05460"
     CDS             14054..16909
                     /locus_tag="PKN53_05460"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOD88431.1"
                     /translation="MEGEDDHISFQKRLTDLYRRGMKELLEKKVTDVSDDEFDTRFKQ
                     TVDEQYREEIKNILTEIRLKKNNEFAIKEVYDDASFEDNAKVVKEVVELLQVYQIRYN
                     YRQQFLSDFFELLLTTGLKQESGQFFTPVPIARYIIKSLPIREITLQKIEQGSKNDLL
                     PNVIDYAAGSGHFVTEVMEVIQEYIDTLTENQFSTPTTKKALRSWQGDQFNWAEQYVY
                     AIEKDYRLVKTAKVSCYLHGDGLAKVIHGDGLGDFATSNDFKDLLKYTDKTYPQDNKQ
                     FDIVISNPPYSVSAFRGTMMDEEKALKAFELYSRLTDQSSEIECLFVERTKQLLKDGG
                     VAGIILPSSILNNTGIYTQTREIVLKYFDILAITEFGSNTFMATGTNTVTLFLRRRNN
                     ADAFNIEEAVKRFFTNLNDVTINGIEKPVHKYVSYVWEGVNFDDYITLLRKNPNEVVK
                     KHEIFKEYNSKIKAKSDIEKWNAILDLEQEKLLYFILTYPQKLVLVKTGEKAEEKRFL
                     GYEFSNRRGSEGIHPIQRGKFIDECTQLFDADTFDNPEKASTYIYKAFQGEFDLDIPE
                     ILQKNVSYQNLVDMLSFDRVTFEKNISLTTKKKVNYELIWKSDSLILLSKIADIRKGT
                     SITKAKTVEGNIPVVAGGQEPAYFHNEANRDGNIITVSASGAYSGFVNYFKNPIFASD
                     CNTINSKNENVISTKLIFEFLKSIQKTIYSLQRGQAQPHVYAEDLAQIKIPLPSLNIQ
                     HKIVSEIEVLEKKEEKTRKEIENFSHKITELYETALSKAKTILRLNDDTIFEVSIGKR
                     VLKNEFVPNGKIPVYSANVIEPFGNINKLLIEDFSKPSVLWGIDGDWMVSYVPEGYPF
                     YPTDHCGVLRVKNNLVNEKYLAFVLEKEGKAFGFSRTKRASIDRIKGIKISVPTLSEQ
                     EKIVSEIEKIETQIEALENEIVEIPKQKEAILKKWLN"
     gene            complement(16990..17079)
                     /locus_tag="PKN53_05465"
     tRNA            complement(16990..17079)
                     /locus_tag="PKN53_05465"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17043..17045),aa:Ser,seq:tga)
     gene            17584..17859
                     /locus_tag="PKN53_05470"
     CDS             17584..17859
                     /locus_tag="PKN53_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOD88432.1"
                     /translation="MNKAQLIDAMAKEANLTKADTKKALDAFIKTTTSALKKGEKVAL
                     VGFGTLSVAKRAARTGRNPQKPTQVIKIPAKNVVKFSVGSELKKAVK"
     gene            17947..18621
                     /locus_tag="PKN53_05475"
     CDS             17947..18621
                     /locus_tag="PKN53_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOD88433.1"
                     /translation="MDNKFIIDNLSPMVSENRLETFINVLNNRTYYITVVLEDLFQSH
                     NASAVLRSCDCFGIQNVHVIENTYQFKENPDVSLGSSQWLTKFKYNSSENNSIKAIEN
                     LRKQGYRIVATTPHTNDVNLNDLDLEKGKIALFFGAELPGLSQTVLEQADEFVKIPMY
                     GFTESFNISVSAAICLYDIVNRLKNSNIKWQLSELEKEELLAKWLKVSVRESEKILKL
                     LKVRNS"
     gene            complement(18624..21389)
                     /locus_tag="PKN53_05480"
     CDS             complement(18624..21389)
                     /locus_tag="PKN53_05480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOD88434.1"
                     /translation="MNNYTSNIKCCINILLITILIFNFKFSAFSQDSNSKPYHDNILI
                     IKLKADGNRTFTNKDAKNAILPYFKNKSYFRIEQEFPNSQNLNKNNIEKNVDISNIFR
                     ITYLDNTIDPAILANKMSKSNIFEYVEPLYKIELLYVPNDPLNQENQYWLEKVKAYEA
                     WDIHKGDTNIVIGISDTGIEVSHPDLINQIKYNYADPLDGVDNDLDGYIDNFRGWNFA
                     ENNNIVSADVNQHGTWVAGIAGAENDNGIGVSGAGYKCKVLPLKIMNSEGIIENAYQS
                     IVYAAEHNVDVINCSWGSTHYQKMGQDVVNYATNNFDLLIVAAAGNTNSDLRFYPASY
                     DNVLSVAGTQEDDERWAPDNSISQKGSTYSYTVDLCAPATNFLSTGAIDSYTLMYGGT
                     SFAAPIVAGFAGILRAYHPEFTAKQIMALIKAGCDNIDTIAYNIPFAGKLGKGRLNMH
                     KSLSMEIPPAVVLEDIIINENNYAISVGGNFINYLKNVENLTITAEVVNGYANIINPT
                     IFEGSLEQMQSYSSASLILLELDENIPHDYNLAIKINYSAENYTDYQFIDFLVNPSYI
                     DISTEKLNLSITANGRIGYSDSQSSIGNGLNYNNLYDLFYDCGIISGNSAVNLFSSIR
                     QNSDFKNEKYPEIITEEIADQHIRLEFSDSLDLNTKGIYIIENAYSWTEDENFVIIDY
                     DIINTSGYPIENYYFGLFTDWDLIEAAKNSAIFDQEAQMLYAKSNSEQSMNAGIKLLS
                     KQTPIFYALDQADENNEVININDGFSDIEKFYMISNPNFLDSELQTDIVLYTGIGPIN
                     ILAKDTITVGFAIIAEPNLYKFKQAIANASEKYNNVLHPQNVKYEFENQFSIFPNPCS
                     NRLNINTNSSENFEVIIYNSLGKKMQNFNSNFNTNIDLTNYKSGIYLIKIKQGDKEFL
                     NKVSVIK"
     gene            complement(21465..22574)
                     /locus_tag="PKN53_05485"
     CDS             complement(21465..22574)
                     /locus_tag="PKN53_05485"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOD88435.1"
                     /translation="MKKIFLIICLTSFYLLSFSQSQQPAISWDKTVHDFGNFKEEAGN
                     QSYDFIFTNTGNMPLYITNVNASCGCTATDYTKEPVAPGAKGFVRVTYNPRNRPGKFN
                     KSITVTSNSENPTSILRIQGNVTPRDKGIEDLYPRAFGELRLRTSHLAMAQISNTTSK
                     TDTVGIVNMSSEPMTIGFDNVPPHIKISVEPQKLAAKKEDAKFGGMGVITVTYDASKK
                     NDWGFSIDRVDVVINGEKNVQNRLSISATIEEDFSHLTKDELANAPKIEFKNTKFEFG
                     TIKAGEKVSHNFEFTNTGKSDLIIRKIRATCGCTATSPEKMVIKAGESSHITATFNSA
                     GRKGKQSKSITVITNDPTNHTTTLIIMGDVEEIQN"
     gene            complement(22605..23000)
                     /locus_tag="PKN53_05490"
     CDS             complement(22605..23000)
                     /locus_tag="PKN53_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOD88436.1"
                     /translation="MKSILLFVFASVLSLGLFAQQSGPTISWTKTVHDFGIFEEEAGL
                     QTYKFEFVNTGNEPLYITNVRASCGCTATDYSKEPLKPGEKGFVSASYNPAGRPGKFN
                     KSITVTTNEFQPTSVIRITGEVIPKKTNN"
BASE COUNT         8142 a         3303 c         3822 g         8083 t
ORIGIN      
        1 aaaatctcgt atagagaaac aacaggagga attgttgtga tagcaaaagc actctctact
       61 aatttgacag atataaaagt taatgataag tctttattta ttattttaga gtctgttgaa
      121 aaacctggaa atcttggtgc tattgctaga attgctgatg ccgcaagtgt cgatgctata
      181 attgtttgcg accaactaag cgatgcctat aaccctaatg ccattagagc tagtttaggt
      241 tgtgtgtttt cagtgaaaat tgttaattcc gaaaccgata ctgttttaaa gtggttaaaa
      301 gaaaataata tcacttctta tgccgcagaa ttacaagcca cagaatttta tcataattgc
      361 gatttttcaa aatcttctgc cattgtgttt ggcacagagg cttttggtct gtctcaaaaa
      421 tggattgatt cggcagataa aagaattaaa attccaatgt tgggaagtat tgattcttta
      481 aatgttgcaa actctgtagc aataattact tttgaggcta gacgacaatt aaattttctg
      541 gtttgattat tgtagtataa tttagtaatg tttaaatttg aattataatg aagaatttta
      601 aaatatttta tttgttaaat gtgttgattt tcatgacatt ttgtagcaat caattgtttt
      661 ctcaaactaa agcttttgta gaaatcactg caaaaactaa acaatcttat cttgatagag
      721 gatatacatt ggtggatttt aaaagcgatt cgattattga tacgaaacct ttagtttctt
      781 cagacatagt cttgaactat aaaacctatt atattgtaca tgttcaagtt gatgcttgtg
      841 cttattgtga atataacctg tattttgttg atacagaaaa tgttatgact gagttagaac
      901 cagagattgt tgtggaaaac aatttaaaac aagcaattta tagatttgat aatgaagaaa
      961 attctaaagg aaaatatgtt gtgctattaa attcaaaact tccttattat gcaaatattt
     1021 ttgtgtttaa gaagaaattt taatagtctt cgtaaataac ttcttcttcg ttgtttaatt
     1081 gtccaataac tttaaattca gtacgtctat ttttttgtct tccttcggga ttatcacttc
     1141 catcaggatt tgagttagga gctatcggga agtttttacc ataacctttt gcaaccaatc
     1201 gttctgattc aatgcctttg ttaattaaat aatcaacaac agattgtgcc ctagcttgtg
     1261 aaagcttcat gttaaactct tcagtatcaa tagagtccgt atgagaacta atctctataa
     1321 ttatatctgg atacgcttgc aaaattttaa aaatagtgtt gtctatagtt tttttagctt
     1381 ctgtagtaag ttctgatttt gcaaattcgt aataaatatt tttaacaata aaagattgtt
     1441 caggtaatgt gtttacaaaa atagcatcta aaactatagt atctgatctt gtaatgtttt
     1501 ttgttgtgaa aggtaatttt ttctcctttt tattgtaatc ttttacagaa ataaaataat
     1561 caaaaccttg ttctaaatta aaaaagtaat attctggtgt tgtaaaatca tttttcacaa
     1621 aaagctcttt accactattt ttatcaatca taaacaagct aacttgatag ttgtataata
     1681 gttctatatc atcacttctg tccaacacat ttaaacttaa agccatttct tgttgatatt
     1741 ctttttcaat agttttgaaa aaagcagagt ctgtaatacc aaaaattttg cctgtaactg
     1801 caatattgat ataatcttta tcaataaact catatacatc gtcgcagcag tttgcgtgtc
     1861 ttagagaata gcctccatct ctgtttgatg taaaaaaacc tctttgtcca ttgttttcta
     1921 aaataaaata caaatcatca aaacttgagt ttataggctg tctaagattt aatgcgtttt
     1981 caaaattttt accatcgcca aaagctttga atatatctag acctcccatt ccagggtgtc
     2041 catctgaact aaaatataaa gttttagttt caatattata ataaggtgta agttcattgg
     2101 caggagtgtt aattctcttt ccacaatttt tgggttcttt ccattcctta cgttttgaat
     2161 cataatacgt aaaccatata tctaatccgc cttgtccatt ttctctgtca gaaacaaaat
     2221 aaagaacatc tgtatttagt cttgaaacac caacagttgg cattgtgtta gtactgccgg
     2281 gtaggttaat tgtttcggga agttcttgag gatcttgcca tacattatct tcataatttg
     2341 aaacaaaaat tttacaaaca actttattat taaaatcttt agcacatctt gtaaaataaa
     2401 atcttttttt atctgcagaa aaagcaccat tgccagtatt tacgttagaa ccattaattg
     2461 tttcatcaaa ctctccaaga tttttccaat cttcaccatc tcttttagcg atataaaact
     2521 ttctaactgg tagttcatct ttatttgggt cataatattt tacttcctct tcagctaatg
     2581 acccccaaat tagtttatta tcttcgaatg gaataggaga aaaatcaata tgaggcttgt
     2641 ttatttcttt atccaaaagt tttacaatag tattgttagg cttattttgc caaagttcag
     2701 ttgagtcaat tccttttaaa tggtttctag aaattttaat ttcttctgtt aagtctttat
     2761 tcttttttgc ttgattaata aaactattaa actgcttttt tgcttcatca tattcgccta
     2821 gagttttaag gcattctgca tagtaaaata aagctttaag gttttttttg gggtctaatt
     2881 tataagagtc aaataaatat ttttgagctt cgcgataatt tttttctttt aaatataatt
     2941 ctcctaatgc agcttggact tttggtctgc cagggtttag ctcttcataa gttttataat
     3001 aatcgattgc agaaaaaata tctccttctt tttgtgctgt ttttgctagt tttaagattt
     3061 gtcctggtct atatgttatc gatatttcat tttgattatt tttttgagca aaaactaaat
     3121 tcaaagtgaa taataaaaag caaaaaacta taaaatatct caacatcttt atcttgttta
     3181 aattcatttt ttaatatcta tcacatggta ttgtaacgct tttaactttt tgtgttagat
     3241 gcttataaat aatcgaaatt tcaaaagctc ctctgttttt tgttgcagct ttaagagcag
     3301 aaatattcac atcatagcta aatcctaggt caaatccgta aatacctaaa ccagctgtga
     3361 aaattattgc gtcagcatta ttaagagaat ttctttcata aatacctgca aaaatattgc
     3421 taatttgttt atgaggcttt aatttaaaat ttgcaattcc acctaaaaca aagtcgttag
     3481 tttttttgtg aagcattact aaaatatttg gaacaatatc aatattgtct tttattggca
     3541 aagttactcc agaattaaga gcaactcgca taggaagttt gttatctgta tttacaaatg
     3601 tttctttagg gttgtttaca tgaaaaaatg ataatccaaa atgtggagtt aaatttttga
     3661 tggtaacttt atattttatt cctaaattta tatctaaata ttgtatgttt tctactgttt
     3721 gtgtaatgtt gtttggtaaa ttggagtcga aaagaccagt attatcatta aattgtgaag
     3781 gcagacttaa tccgtttaaa gtaaaatttt ttgtaacaaa tccgatttga gcgccaaatg
     3841 ataaaacatt agtttcgttt aatgaaagta tataacttcc gttaacaaaa attttattta
     3901 ctaataaatt acttgagcct gaatggtcgt ttataaaaaa tgcaccaaga ccaaaatttt
     3961 tgcctttttt tgtgtgcatt gacttgtcaa atccaacaga aatagtgcga taaggagtgc
     4021 caattgcagc ccattgtgtt ctataattgt tggtaaatcg ccaattttca tcaaaaaaac
     4081 cagtctccgc aggatttagt gagagtggag aagcagtaaa ttggctaaaa tgtatgtctt
     4141 gacaatatgc ttttgtgcaa aaatagccaa aaattaaagt tactgttgat ataataataa
     4201 aaatcttttt caatgccgtt caacttttta taattatgat tttcaaaaat aataattttg
     4261 ttttaaaaaa caaacttttt tatgattttt ttttataaaa aaatatttaa taaaatttcg
     4321 ggaaattatg ctagaataat taaaattata ttatttttgt taattgatgt tttgttttac
     4381 taatgcattg cttaaaatga aaagaattta tattttattt ttgttaattt tcagtgttac
     4441 gagattgttt tcgcaaccta cagctgattt tacttctagt gggaataatt tatgtgcgcc
     4501 agcaattata acctttacta atacaagtac tggatgttcg ggtacaccta ccttttattg
     4561 gacatcagga acaagcgatg tgtcttctct tgaaaatcct acattttctt atacagtagg
     4621 aggaacttat acggttagtc ttaaaattac ctgtgatgga tttgaagaca caaaaactat
     4681 gaccattgta gtttatgatc cacctgaggc aaactttagc aatgtagtgc agaaaggatg
     4741 tgttccattt gatgtgaatt ttatagacat gtctttaaca ggtgatgccg caataaattc
     4801 atgggtttgg cattttggag atggaacaag ttctacagag caacatcctt cacatactta
     4861 tgcttcaagt ggtagtttta atgtaacttt atttgtaaaa gatgaaaata attgctcttc
     4921 acaggttact cataataatt taataagggt tgctaatttg cctacagtta gttttgtggc
     4981 tgataatcca actttgtgta cctcacctca tacggttaat tttagctcgt ctgttactac
     5041 ctcgtttggt ttaacagcaa cctatttatg gaatttcgga gatggcggta caagcataga
     5101 aaataatcct agccatacat atacttcagg aatttatgat gtgagtttaa cagtaactga
     5161 tgaatatggc tgtgaaaata cattttctaa acctgaatat gtgagaatag aaccagcagt
     5221 cccccagtat tcggttttag agggcgatac agtatgtaaa ggaatgcaaa cacatttttt
     5281 aaacgaaaca ggctattcgt gtcgctggga ttttggagac ggaggtacaa gtatgcaaaa
     5341 tactccaata catatttatc agcaaacagg aaatgttaat gtaactttta ctgttgaccc
     5401 aggtggacaa tgtgaggctt caacggaatt tactcttttt gtagaaacta ttactgcatc
     5461 tttcacaact aacccaacaa acttatattc atgctcaata ccctttgagg tagaatttac
     5521 aaatacttca tcagcaaatg ctacaagttt ttattatgtg tttcaagatg gaacttcatc
     5581 aattcagcca aatccttcgc acacttattc atcaggagga atatttcagc caactttaac
     5641 agtaacaagt aatgcgggct gtgtacatac atttatggga cctataataa atatttcacc
     5701 cgcagatgct agctttacag ccgatgaggt tgaaggttgc gaaccattaa ctattacctt
     5761 tacatattca ggagatccag aggctattac taattttaac tggaattttg gaaatggaaa
     5821 cacaaactca gatggttctg ctatagaatc ttcggtttat aatgcaggag aatatacagt
     5881 tgtattaaca gttacagatg gacaaggttg cactggagaa tcaagtcttg aactaaatat
     5941 aggagctgtg tatgaaccaa acataaatgt ttttgataat gatcctgacc acacaccttt
     6001 gttaagccat aatatttgtg cacaagatac tgtctcgtta tgggtgttgg aatgggataa
     6061 tgaggactat gaatttactt ggtggataga ctccacatca aataaagatg tgtcaaatga
     6121 atacactgat catgcttttg accaagatac aggttgggtt cacttgcaca tgataacttt
     6181 gtataatggc tgtagagata caatttattg ggatagcctg tatataagtg gacctattat
     6241 tgatggtata tctaaatctt atgattgtaa taatcctaga aattatgaat ttactttaaa
     6301 tgaaactata gctgatactt gggattggaa aatatattat attcatgaag gtacaatgac
     6361 aattgtggaa gagactctga gctcaacaga tcataattat tcatatactt ttccaccaag
     6421 tccagattca ttttgggtta aggtaacagc acatagcgat acaactagtt gcgaatatgt
     6481 ggactcaata caaattaaaa tagcaacacc gagtgcaagt ttttctatac ccgtatataa
     6541 taattgtaca ggagttgaca tatcttttga tgcttctgct tctcaagatg caagcgaata
     6601 ctattgggat tttggagatg gaaataattc aggttggtta agtgaaccta tatttatata
     6661 tcaatatatg actgtcggtg tgtttgatgt agtgttaact gttcgtgatg aaaacggttg
     6721 tgaagatact gatactcacc ctatacatat tattggtccg gaaatacata caactatcag
     6781 cgaaacatat ggttgcgacc aattattagt gagtttttca gacttttcta cagctagtga
     6841 accaatagta agtgttgaat ggaattttgg agatggctcc acaatgatag gcacagatgt
     6901 aacacattta tactcaacac caggcgttta ttctgtaact gtgaatgtaa caactttatc
     6961 aaattgtggt ttagggatta cttatcagga tacaattgtt gtcgaatctg ttgacgctag
     7021 ttttaatgct ctgccagttg ttgcttgtgt taatcaagat attagttttc aagctttaga
     7081 aaataatata agttatatat atacatggaa ttttggagat acaaatcctg caatttctgg
     7141 acatagccct tctataaatt attcatacga aaatggtgga tattatgacg ttcatttaaa
     7201 agtagaaaat ggaagggggt gcgaagatga gcttttgtta gagggcttta taaaaataga
     7261 gcaggcattt gctaacttta gtttaactga agatcatttt tcatgttatc cagctacgcc
     7321 aataataatt caaaattcta gtgttttgcc agccgataca gaattatatt atcaatggat
     7381 tttaggcagt gataatgata caattactgt tgaagaaccg acatatgtat atactgtgcc
     7441 tgggaacttt gatattgttt taaatttgtc aacgccagca ggatgcaccg acacacattc
     7501 tttaccatta attattgaag gtccatatgc acaaatgaat attagtgata ccatagcttg
     7561 tgttggacaa gaaatagaat ttagccttac agaacaagaa aatgttgaaa ctatacaatg
     7621 ggttgtcgga gggggagatt cttatactgt agaatcattc actcattcgt attcaatggt
     7681 gccaccaagc ggttattata cagttaattt agtgataaaa tcaggagatt gtcaaccacc
     7741 ttcgtttgtg caaaatattt atatttttga tgttactgca gattttagta ttacagatac
     7801 agaaaataat gaaatactag gtgggctttg ttctccattt gaggcaatgc tagtaagtaa
     7861 ctctataaat gacgatttta gatattggtt tgttgacaac catccaatag gatccggttt
     7921 aagtactgaa actcataatt ttgtaaataa tcaaagttat gatacaagtg gagaaataac
     7981 tttaattgtt gaagatattc acggttgcct tgatactatt aaaaagagca ttgatgttta
     8041 tgccttgcct gtaattgaaa taactcaaga tactattatt tgtaaaggtg atgaaatcaa
     8101 gttatattct gcaggaggtg taagttactt gtggtctcca gatgtcaata tttctgatgt
     8161 taattctcaa tcgccaagtg taaaaccaga agaggatatt atatattatt tagaagttag
     8221 aaactcgcat gattgtctta gtatagattc tgtgagtata ttagtaatac aggatttttt
     8281 agtaaacttt acacctgttt atgattctat tattattgga gatactacac gaattatttt
     8341 agaagctact caagacagtt taatttatac ttggacacct agtacaaata tttcatgtac
     8401 aaattgtcca gaacctgaat tttatccact cgaagataca agatacagac ttgtagtaga
     8461 agatagtaca gggtgtttca agtatcatta tttgctcgat atttttgtaa ttgaggaata
     8521 tacattagat gtaccaggag cgtttacgcc tggttctggg gataaaaatg ctgtaattta
     8581 tgctagaggc tttggaatta aaaaattatt gcagtttaga atttataacc gttggggaga
     8641 agaagttttt tatactgacg atataaacca aggctggaac ggatattata aaggaaagtt
     8701 acaaagtata gataattatt cctattttat tgaagcagaa atgtttaatg gaagtattca
     8761 aactaaaaaa ggacatataa tgttgattag ataagaaact tgttttaaat tctaaattct
     8821 caattcaaaa aaacttttta tttcccatat tttttcccaa ctttgcagaa aatacaatta
     8881 tatgataatt attacaggtg ctgcaggatt tatcgctagc tgtttagtta cagaacttaa
     8941 taaccgtggt tataaagacc ttgtattagt tgatgatttt agtaaaagtg aaaagaaaaa
     9001 taactattca gataaaactt ttacaaaact tatcgataga aacgaatttg attattggtt
     9061 ggatagaaat cacaaacttg ttcaattaat ttttcatatt ggtgcaagaa cagatactac
     9121 cgaatttaac tttgaaatac ttgaaaaatt aaatttagaa tactcaaaac ttatttggcg
     9181 aaaatcttat gaatatggtt tgccattaat ttatgcatct tcggcagcaa cttatggagc
     9241 cggagaattc ggttttgatg acaatacttc tccagaatta cttaaaccac taaaccctta
     9301 tggaatttct aaaaatgaat ttgataaatg ggctttgaat aatgagaaaa aaccatattt
     9361 ctgggctgga tttaagtttt ttaatgttta tggtccaaac gaatatcata aaggtagaat
     9421 ggcatctgtt gtctatcatg cctacaatca aattaaagaa acaaaaaaat taaaactttt
     9481 taaatctcat aatccaaagt ttgaagatgg aatgcaattg cgagatttta tttacgtaaa
     9541 agatgtttgc tctgttttaa ttcattttat ggagactcgc aaaaattcag gaatatataa
     9601 tcttggaact ggaaaggcaa gaagtttctt agatttaggt aaagcttgtt tcaatgcact
     9661 aagtatagaa ccaaatatcg agtttgttga tattcctttg gatataagag ataaatatca
     9721 atattttacg caagcaaatg ttgaaaaatt gcgttcagta ggttatgata aaccatttta
     9781 tagcatagaa gagggagtta atgattatat ttctaattat ttaaaagaag aaaaatattt
     9841 ttaagaaatg agcaataaaa aaaaatatat aggcgaagta gaagaggcta aagcagataa
     9901 ttctgctttg aaagtaaatc aaggaataga gcaaccacct caagtaaatc ctaattttag
     9961 aaaatttaaa agaataaaaa aagatttaac agtttctgat tatgttgaag gaattgttgc
    10021 aggtgatgta aatattttaa gtcaagctat tactttagtc gaaagtcgtt tagccaaaca
    10081 tcaagaaatt gctcaagaaa ttattaaaga atgcttgcat ttagataaaa aatctattcg
    10141 aattggaatt actggagttc ctggagcagg gaaaagcact tttattgaag ctttgggaat
    10201 ggaaattgta aatcgaggac ataaattagc agttttagca gttgacccaa gtagtgagcg
    10261 tagtggtgga agtattttag gcgataaaac acgaatggag gaattgtcgg ctcagaaaaa
    10321 tgcttttatt cgtccatcgc cttcggcagg ttctcttggt ggagtagctc gtaagacacg
    10381 tgaaacaatt gtgctttgcg aagcagcagg gtatgataca atttttattg aaacagttgg
    10441 tgttggacaa tccgaaactg ctgtgcatag catggtagat ttctttttat tgctaatgtt
    10501 atctggagct ggtgacgaat tgcaaggaat aaaacgagga attatggaaa tggctgatgc
    10561 tattgcaata acaaaagctg acggtaataa tgtgcaaaaa gcaaaactag cacgagttca
    10621 atatcaaaat gctttgcatt tatttccaca aactgaagca aaatgggaac caaaagttct
    10681 gacttgttca tcaattgaga aaaaaggaat aagtgaaatt tgggaaacaa taaaggaata
    10741 tgaaactcat acaagaaata atgggtattt cgagaataat cgcttaaaac aatctaaata
    10801 ttggttgttc gaagcaataa attcagcttt gaaagaaaga ttttatgacg ataaattagt
    10861 gaaaaccgaa ttgagaaaat tagaagttaa aatcgaaaaa aatgaaataa gtagttttgt
    10921 agctgctacc caattgttag atatttattt tcaaaagaaa tgataggaca aaaaattgtt
    10981 tacgaaacct tagaaaaact gaatataagc tttgaatatt atgaggcacc aaaagatttt
    11041 tcaaatgaag aagatataag tttttggaat aggataggag caacacgttg taaaaacctg
    11101 tttatgagaa atcataaagg gaatagacat tttttgttaa tttctgatta ttacagagat
    11161 attaatatta aagtgttaga agataaattt aaaaaaggaa aaatttcttt tgcatctcaa
    11221 caaagaatag ataaacacat aaaaggagtg ataggtgcaa tttcagtttt ttctttatta
    11281 aacgatgagg ctaatcatgt tgaggtcttt attgatgagg gattgaaaac aaaacaaaaa
    11341 cttacatttt taccaaacga attaaatgct cttattgcaa taagttttga agattttata
    11401 aaaatccttg attattctgg caataaatat gaatttatga atttagcagc ctcggttgag
    11461 taattatttt actctaattt tttgaccaat ttgtaattta gaatcttcgc taattccatt
    11521 cattgaacat atttgactaa ttgaaatatt atattttcga gaaattgccc aaagagtatc
    11581 gccgctataa actatatgat aattgctttc caaaacccca gacactccac cagaagttct
    11641 acgtctcgat tttaaatatg caaattcttc tggcatcaac accaaagtat cagattttag
    11701 tttattgttt tcaaaatcta ttattaattc agggttaaaa ggattgtctt taaatctggt
    11761 ttcaaaatga agatgaggtc cgtaagattt gccagtattt ccacctcgag caattgtgtc
    11821 tccagctttg acctcttgat ttctttgaca taataatttt gacaaatgtg cgtaataagt
    11881 ttcaataccg ttgtaatgtc taataataac caaatatcca taacctcctc tgttccaacc
    11941 tgcataacgt acaatcccat caaaagcaga aacgatggta tctccagttt ttagcctaat
    12001 gtcaattcca ttatgatgat atcctcttcg ccacccaaat ttactgatta cagttcctat
    12061 atatggcatg taaaattttt ccccattatt taataaactt atagcacaag tgtcaagagt
    12121 tcgtttgtaa tcagccttgc caaaggcaaa aatattaatt gtatcccagt tttctgaaaa
    12181 tacagttaaa ctatctaatt ctaaaagtct attttcgtaa atatcattat aaagttttat
    12241 tgagtcgttg aatttaataa cagccaaaac actatcttga tttatatatt cccaagtgtt
    12301 gttttcaaat aaaataatat caaaaccttt actatttaca gtgtctatta ttatgcgagc
    12361 atttttatcg ttttgtgaaa aaatcaaatt aggaataaat ataagactta gaaatatgta
    12421 aaaaagatgt ttcatatttt tctattttga attaacaaaa atgaaaaaaa taatctaaaa
    12481 caaattattt atcagaaatt ttatacaaat agtgaatatt ctcctatttg tatgattttt
    12541 aaagttactt ctttgatgtt ttaggtttta aaaatgataa ttctcaaaat gtttcgatta
    12601 taattaagat tgaaaaaaac tttgtttgct aaataaaact tattattttt gcaatgcaat
    12661 tttgcggggt gtagctcagt tggttagagt acgcgtctgg ggggcgcggg gtcggtagtt
    12721 cgagtctact caccccgaca aaattaaaaa atcaaaaatc ctaataaata atccgaaaaa
    12781 ttaaaatttt agagcgttgt taataaattt gtattaaata ttttaatttt tacaaaaata
    12841 tattagaaaa tttgcctaaa aaagataaaa caaatttgaa taaaacatga aaatgacaag
    12901 ttttggtaaa tttataaaag cagaacgaga aaaaagaggt tggagtcaaa cagaattagg
    12961 tgctttaatt aagataaata ctccacttgt gagtcgtatc gaaaacgata aaaagctcat
    13021 gtcttttgat aaagttgagc tacttgctaa tgcttttgaa atggattatc aggaattaaa
    13081 agatctttat tttgcagata aaacagcaaa atctgcttat gaatacaaat gttctaaaaa
    13141 cgtttttgca gttgctgaaa aacaatatga atatatagaa gctaccaaca ttgtacaagg
    13201 acaattaaaa ttctaatcgt gatggacaat aagaaattaa tacagacatt gggatttgta
    13261 ccaaaagaaa acagttcggg tatttttcag aaaaaatatt ctaaagctga ctattatgtt
    13321 gttgaaattg attttgagaa agaaaatatc aactatggaa ataaaataaa aggtgaaagt
    13381 aaaacaacgc aaaacttttt acaggctgaa aactgggttg ttttggagtg tgtggatagg
    13441 cttttgggaa aagggtatca gccacaaaat ataacattgg aaaaaacttg gaaaacagga
    13501 cacggaacaa gcgggcgatt agatattttg gtaactaaag acgatggttc cgcttattta
    13561 atgattgaat gtaaaacttg gggcacagag tatgaaaaag aactgaaaaa cttagagaag
    13621 aacggcggac aactttttac ctattttcaa caagataaaa atgccgatgt tctaatgcta
    13681 tatacttcta aaatagacag taaaggtgtt gaatacaaaa atacaattgt aaaaattgag
    13741 gaagattaca aacaaactgg caatgtaaaa gatttttacg aacgttggaa taagcttccc
    13801 aaaacaaacg gtatttttga tagctgggtc agaccttacg aatttcaaag taaagcgtta
    13861 acaagaagag atttaaaggc tttaaaacaa aaagatagta gttttatttt taatcgcttc
    13921 ttggaaatcc tacgtcacaa tgtagtgtcg gataagccta acgcttttaa taaaatattt
    13981 acactttaca ctatttctgt gtaaaattat tgatgaagat cgaagtgaag acgaacagct
    14041 tcatttccaa tggttggagg gggaagatga ccatatctct tttcaaaaaa gattaacaga
    14101 cctgtatcgt aggggaatga aagagctatt ggaaaaaaag gttacagacg ttagtgatga
    14161 tgagtttgat acacgtttca agcaaaccgt agatgagcaa tatagagagg agattaaaaa
    14221 tattttaacc gaaatacgtc tgaaaaaaaa taacgaattt gccataaagg aagtgtatga
    14281 cgatgcttcg tttgaggata atgccaaagt ggtaaaagag gttgtagaac tcttacaggt
    14341 ttatcaaatc cgttataatt atcgtcagca gtttttaagc gatttctttg agttgctttt
    14401 aaccactggg ttaaaacaag agtcgggaca gtttttcaca ccagttccaa tagctcgata
    14461 tattattaaa agtttaccta ttcgcgaaat aacattgcaa aaaattgaac aaggaagcaa
    14521 aaacgatttg ctaccaaatg ttattgacta tgctgctgga agtggacact ttgttacaga
    14581 ggtaatggaa gttatacagg aatatattga taccttgacc gaaaatcaat tttcaacacc
    14641 aacaacgaaa aaagccctta gaagctggca gggagaccag tttaattggg cagaacagta
    14701 tgtatatgct attgaaaaag attatcgttt ggtaaaaact gcaaaagtaa gttgttactt
    14761 acacggagac ggcttggcaa aagttattca cggagacggt ttgggagatt ttgctacatc
    14821 aaacgatttc aaagatttgc tgaaatatac cgataaaact tatccgcaag ataataagca
    14881 gtttgatata gttatttcaa atccgccata ttcggtttcc gcattcagag gcacgatgat
    14941 ggacgaagaa aaagctctga aagcctttga actttatagc cgtttaaccg atcaaagttc
    15001 agaaatagag tgcctgtttg tagagcgaac caaacaactg cttaaagatg gcggtgttgc
    15061 aggtattata ttgcccagca gtatattaaa caatacaggc atatataccc aaactcgcga
    15121 aattgttttg aaatactttg atattctggc tattaccgaa tttggttcta atacatttat
    15181 ggctaccgga actaatactg ttactctgtt tttacgaaga cgaaacaatg ctgatgcctt
    15241 taatattgag gaggcagtaa aaagattttt tacaaatctg aacgatgtta caatcaatgg
    15301 aattgaaaaa cctgtacata aatatgtgtc atacgtttgg gagggggtaa attttgacga
    15361 ttatattact ttgctcagaa aaaatcctaa cgaagtcgta aaaaaacacg agatttttaa
    15421 ggagtacaac agtaaaataa aagctaagag tgatattgag aaatggaatg caattttgga
    15481 cttagagcaa gaaaagctac tctattttat tcttacttat cctcaaaaat tagtacttgt
    15541 aaaaacaggt gaaaaggctg aagagaaacg ttttttaggc tacgaattta gcaaccgccg
    15601 aggcagcgag ggaatacacc ctatacagcg aggaaaattc atagacgagt gcacacaact
    15661 gtttgatgct gatacatttg acaatcccga aaaggcaagt acctatattt ataaagcatt
    15721 tcaaggagaa tttgatttag atattcccga gatattacag aaaaatgtta gctatcagaa
    15781 tttagtggat atgcttagtt ttgatagagt tacctttgaa aaaaatattt cgcttactac
    15841 taaaaaaaaa gtaaactatg aactcatttg gaaaagtgat tcattaatat tgttgtctaa
    15901 aattgcagac ataagaaaag gtacatcaat aacaaaagca aaaacagtag agggcaatat
    15961 tcctgttgtt gcaggaggac aagaacctgc atattttcat aatgaggcta acagagatgg
    16021 aaatataata acagtaagtg cgtcaggagc ttattctggt tttgtaaatt attttaaaaa
    16081 tccgattttt gcttcagatt gtaatactat caattctaaa aatgaaaatg tgatttcaac
    16141 aaaattgatt tttgaatttt tgaaatcaat tcaaaaaact atttattcac tacaaagagg
    16201 gcaggctcag ccacacgttt atgcagaaga tttagctcaa ataaaaatcc ctcttccgtc
    16261 actaaatatc caacacaaaa tcgtttcaga aatagaggtt ttggagaaaa aagaagaaaa
    16321 gacaaggaaa gaaatagaga attttagtca taaaataaca gaactatatg agactgcttt
    16381 gtctaaagca aaaacaatat taaggttaaa tgacgatact atttttgagg tttctattgg
    16441 aaaacgtgtt ttaaaaaacg aatttgttcc taatggaaaa atacctgttt atagtgcaaa
    16501 tgtaattgag ccttttggaa atataaataa attattaatt gaagatttta gcaaaccatc
    16561 tgttttatgg ggaattgacg gagattggat ggttagttat gtgcccgaag gttatccatt
    16621 ttatccaaca gatcattgtg gagttttaag agtaaaaaac aatttagtta atgaaaaata
    16681 tttagctttt gttttggaaa aagaaggaaa agcttttggt ttttcaagaa caaaaagagc
    16741 ttctattgat aggattaaag gtattaagat ttctgttcct acactttccg agcaagaaaa
    16801 aatagtttcc gaaatagaaa aaatcgaaac gcaaatagag gcattagaaa atgaaatagt
    16861 tgaaattccg aaacaaaaag aagcgatctt aaagaaatgg ttgaattaac ttaacaataa
    16921 aaaaaggact aaattttact tagtcctttt tttatttttc atcaagtaaa ttacttactt
    16981 tttatgatgt ggcggagaaa gagggattcg aacccccgga gccctctcga gctcaacggt
    17041 tttcaagacc gccgcattcg accgctctgc catttctccg ctgcaaaagt agattaattt
    17101 attaatatgc aaaaatattt tgaatgtttt ttttgaaaaa aactgtaatt atattataaa
    17161 cctttaatca tgaggtatat acataataaa ttttttttaa ctgagattat tttgaaaaat
    17221 cacacctatt aaatacatat ataaataacg tgttttaata ttcgcgaaaa accgattttc
    17281 ttgtttagtt gaagaaaaat gacgttttac acgttttttt aagttaaaaa cactttaaaa
    17341 cacttgaaac ataggggttt tagtaaattt ttataatgaa aaggcttggt tttgtttagg
    17401 tgaaacggct gaaatgcttg tattttcaat attttgcagt tgtttttgct tgggttatta
    17461 acaaaataaa taagttatta acaaaagtga aaaaacttga ttttaaatta ttgctataac
    17521 ccttattatt actatatttg ctttgtaaaa ttaatcttat tattaatcta aaaaattaaa
    17581 aaaatgaaca aagcacaatt aattgacgca atggctaaag aagctaactt aacaaaagct
    17641 gacacaaaaa aagcattaga cgctttcatt aaaacaacaa caagcgcttt aaaaaaaggt
    17701 gaaaaagttg cattagttgg atttggtact ttaagtgttg caaaaagagc agctagaact
    17761 ggtcgcaacc cacaaaaacc aactcaagta attaaaatcc ctgctaaaaa tgttgtaaaa
    17821 ttctcagttg gaagcgaact aaaaaaagct gttaagtaag aattgcatca ttaatgaaat
    17881 taaaaaagag agttatagtt tttataactc tcttttttta tatttgcctt ttcaaaaaac
    17941 cgtataatgg ataataaatt tattatagat aatttatcgc caatggtttc tgaaaaccgt
    18001 ttagaaactt ttataaatgt attaaacaat cgtacctatt atataactgt tgtactcgaa
    18061 gacctttttc agtcgcataa tgcttcggca gttttgcgtt cttgcgattg ttttggaatc
    18121 caaaatgtac atgttataga aaatacttat caatttaaag aaaacccaga tgtttcatta
    18181 ggctcatctc aatggttgac aaagtttaaa tataattcat ctgaaaataa cagcataaaa
    18241 gcaatagaaa atctaagaaa gcaaggctat agaatagttg caacaacacc ccataccaat
    18301 gatgttaacc ttaatgattt ggatttagaa aaaggtaaaa tagcattgtt ttttggagca
    18361 gaattaccag gattatcaca aactgtatta gaacaagcag atgaatttgt gaaaatacca
    18421 atgtatggtt ttacagaaag ctttaatatt tctgtgtcgg cagcaatttg tttgtatgat
    18481 attgtaaatc gtctaaaaaa ttctaatatt aaatggcaat taagcgaatt agaaaaagaa
    18541 gaattactag ccaaatggct taaagttagc gtgagagagt ctgaaaaaat tttaaaatta
    18601 ttaaaagtta gaaattctta gttttatttt attacagaaa ctttatttaa aaattcttta
    18661 tctccttgtt ttatttttat taagtaaatt cctgatttat aatttgttaa gtctatgttt
    18721 gtgttaaagt tagaattgaa attttgcatt ttttttccca atgaattgta aataataact
    18781 tcaaaatttt cagaactatt tgtatttata tttaatctat ttgaacaagg atttggaaaa
    18841 attgaaaact gattttcaaa ttcatattta acattttgag gatgaagcac attgttatac
    18901 ttttcgcttg catttgcaat agcttgttta aatttatata aattaggctc agctataata
    18961 gcaaaaccaa ctgtgatagt atctttagcc aagatgttaa taggaccaat tcctgtgtaa
    19021 agaacaatat cggtttgtaa ttccgaatca agaaagttag gatttgaaat catataaaat
    19081 ttttcaatat cagaaaaacc gtcatttata ttaataactt cattattttc atcagcttga
    19141 tctagagcat aaaatattgg tgtctgtttg cttaataact taattcctgc attcatgctt
    19201 tgttcggaat tactttttgc gtaaagcatt tgtgcctctt ggtcgaaaat agcagaattt
    19261 tttgctgctt ctattaaatc ccagtctgta aataaaccaa aataataatt ttcaataggg
    19321 tagccacttg tatttataat atcataatca ataataacaa aattttcatc ttcagtccaa
    19381 gagtatgcgt tttcaataat ataaattcct tttgtattta agtctaagga gtctgaaaat
    19441 tcaagtctaa tgtgctggtc tgctatttcc tcagtaatta tttcgggata tttttcattt
    19501 ttaaaatctg agttttgtct tattgagctg aataaattta ccgcagaatt tccggaaata
    19561 attccacagt cataaaacaa gtcgtataag ttgttgtaat ttagtccatt acctattgaa
    19621 ctttgagaat cagaatagcc gattcttcca tttgcagtaa tagaaagatt taatttttca
    19681 gtgctaatat caatataact ggggttaact aaaaaatcaa taaattgata atcagtatag
    19741 ttctctgcag aataatttat ttttatagct aaattataat cgtgaggaat attttcgtcg
    19801 agttctagta aaattagtga tgcagaactg tagctttgca tttgttctaa acttccttca
    19861 aaaattgttg gattaataat attggcatat ccattaacaa cttctgctgt tatagttaag
    19921 ttttcaacat tttttaaata atttataaaa tttccaccta cactaatcgc atagttgttc
    19981 tcattaataa ttatgtcttc tagaacaact gctggaggaa tctccatgct taaagatttg
    20041 tgcatattca atcttccttt tcctaatttt cctgcaaaag gaatattata agctatagta
    20101 tctatattgt cgcatccagc ttttattaga gccataattt gttttgcagt aaattctggg
    20161 tggtaagctc ttaaaattcc tgcaaatcct gcaacaattg gtgcagcaaa tgacgtaccg
    20221 ccatacatta gtgtgtaaga atcaatggca ccagttgata aaaaattagt ggcaggagca
    20281 catagatcaa cagtataaga ataagtgcta cctttttgag aaattgaatt atcaggagcc
    20341 catctttcat catcttcttg tgttccagca accgataaaa cattatcata tgaagcagga
    20401 taaaatctta aatcagaatt tgtgttacca gctgcagcca ctattaataa atcaaaatta
    20461 tttgttgcat aatttacgac atcttgtccc attttttgat aatgtgtcga accccaactg
    20521 caattaatta catcaacatt gtgttcggct gcataaacta tagattgata tgcgttttca
    20581 ataattcctt cagagttcat tatttttaaa ggtaaaactt tgcatttata accagcacct
    20641 gaaactccaa taccattatc attttctgca ccagcaatgc cagctaccca ggttccgtgt
    20701 tgatttacat ctgcacttac aatattgttg ttttcagcaa aattccatcc tctaaaatta
    20761 tcaatgtacc catctaaatc attgtcaacg ccatctaaag ggtcggcata attatatttt
    20821 atttgattaa ttaaatctgg atgtgaaact tcaattcctg tatctgaaat gccgataaca
    20881 atattggtat ctcctttatg aatatcccaa gcctcgtatg cttttacttt ctccaaccaa
    20941 tattgatttt cttgatttaa tgggtcattt ggaacgtata gtaattcaat tttgtacaaa
    21001 ggttcaacat actcaaaaat attagattta gacattttgt ttgccaaaat agcaggatca
    21061 atagtgttat ctaagtatgt aattctaaaa atattgctta tgtcaacatt tttttcaata
    21121 ttatttttgt ttaaattttg tgaattcgga aattcttgtt caattctaaa gtaagatttg
    21181 tttttaaaat aaggtaaaat tgcgtttttt gcatctttat ttgtaaaagt tctatttccg
    21241 tcagctttta attttattat taaaatattg tcgtgatagg gtttgctgtt tgaatcttga
    21301 gagaaagcac taaatttaaa gttgaaaatt aatattgtaa tcaataaaat attgatacag
    21361 catttgatgt ttgaggtata gttattcata gcaatatttt tttgcaatat ataaaaaaaa
    21421 tagtcgtatg gaacgactat tttaaaaaaa gtttaaatat gtttttaatt ttgtatttct
    21481 tcaacgtctc ccattattat tagagttgtt gtatgattag ttgggtcatt tgtaataact
    21541 gtaatacttt tactttgttt tccttttcta ccagctgagt tgaaagtagc ggtaatatgg
    21601 ctggattcgc ctgcttttat gaccatcttt tcaggacttg tagctgtaca accacaagta
    21661 gctctaattt ttctaataat taaatcactt ttaccagtgt tggtaaattc aaaattatga
    21721 cttacttttt ctcctgcttt tatagttcca aattcaaatt ttgtattttt aaattcaatt
    21781 tttggagcat tggcaagctc atctttagta agatgtgaaa aatcttcttc tatagttgca
    21841 ctaattgata gtctattttg tacatttttc tcaccattaa ttacaacatc aactctgtct
    21901 atagaaaagc cccaatcatt ttttttactt gcgtcgtaag taactgtaat tactcccatg
    21961 ccaccaaatt ttgcgtcttc ttttttggct gctaattttt gtggttctac agaaattttt
    22021 atgtgtggtg gaacattatc aaaaccgata gtcataggtt ctgaactcat atttactatt
    22081 ccaacagtat cggttttgct tgttgtatta gaaatttgag ccattgctaa atgcgaggtt
    22141 cttagtctta gttctccgaa tgcacgtggg taaagatctt caattccttt atctcttggt
    22201 gttacatttc cttgtatgcg taatatgcta gttgggtttt cactatttga ggtaacagtt
    22261 atagatttat taaacttacc aggtctattt ctagggttgt atgtaaccct aacaaaacct
    22321 tttgcacctg gagctacagg ttcttttgtg tagtcggtag cagtacagcc acaagaagca
    22381 ttaacatttg taatatataa aggcatatta cccgtgtttg taaaaataaa atcataactt
    22441 tgatttccag cttcttcttt aaaatttcca aaatcgtgaa ctgttttgtc ccaagaaatg
    22501 gctggttgtt gcgattgaga aaaacttaaa agataaaaac tagttaaaca aataattaaa
    22561 aatatttttt tcattttaat aaaagtttgg ttttacaaat taagctaatt gttggttttt
    22621 ttaggaataa cttcaccagt aattctaatt acagaagtcg gttgaaactc atttgttgta
    22681 acagttatag atttattaaa ttttcctggt cgtccagctg gattatatga agctgaaaca
    22741 aaaccttttt cgcctggttt tagaggttct ttagaataat cagtagctgt acaaccacac
    22801 gaagctctaa catttgttat atataaaggt tcgttgcccg tgttaacaaa ctcgaactta
    22861 taagtttgca aacctgcttc ttcttcaaaa attccaaaat catgaacggt ttttgtccac
    22921 gaaatagttg gacccgattg ttgtgcaaat aatcctaaac ttagaacact agcaaaaaca
    22981 aataataaaa tacttttcat aatataaaat tttaaattaa aaatcaaatt tatttataca
    23041 ctaataccat tttcaaattt tgtaccaaaa ataattattt tttaataaag agtttaattt
    23101 tacaacaaaa tattaacatt tttaattgaa aacatgcgag ttaaccaatc ttttaggctt
    23161 gctaataaaa aactaatatt tttaataata aaatttctca ctatttattt aatatgacaa
    23221 aattactgat ttttttaaag atattacaat ttttgcaatt tttttttaat taattataaa
    23281 attaatgggt aaattaatta agtgtcccct tttattagga cagttttttt aatttgttaa
    23341 tattttattt
//
LOCUS       DAOPNX010000036        23310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_2748_length_23310_cov_5.669533, whole genome shotgun sequence.
ACCESSION   DAOPNX010000036 DAOPNX010000000
VERSION     DAOPNX010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23310
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2748_length_23310_cov_5.669533"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="PKN53_05495"
     CDS             complement(<1..1293)
                     /locus_tag="PKN53_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD88437.1"
                     /translation="MLTKNSLSKILSASESSHIERIVSTNDMDKFCQAICSFSNDVSS
                     SGKNGYLIIGAHNDGKLSGLKVDDKLLLKISNIRTDGNILPQPTMSVEKFSFEDGELL
                     VVEVQPSEFPPVRYRGRIWVRVGPRKSTATEAEEKILMERRLSNIHTFDAMPCLGTNI
                     NDLDVKIIEKEYLPKAVAEDILAEDNRCIEEQLASLGFYDLRYNCPTNGAIVLFGKNP
                     ERYLHGSYIQYVHFKGKDRAGDIINEHKFAGNLCRELPKIDTFIETSIAQKRPIPVSV
                     LREETFSKYPYWATRELLMNAIMHRDYETNAPVQFYEYDDRIEVQNPGGLYGKVTPEN
                     FPNVSDYRNPFIAEAMKVLGYVNRFSRGVDRVQKELIENGNGKAKFDFSLITAFRVIE
                     NVSEKYLEEGFILEKAKEKAKEKPKKYILVEFPYPSGKS"
     gene            1531..3645
                     /locus_tag="PKN53_05500"
     CDS             1531..3645
                     /locus_tag="PKN53_05500"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOD88438.1"
                     /translation="MSKRKIENKGVFIYLVILFVISVSLVVFIFPRKEQFQYSYEPGT
                     PWLYESLVAETDFPIYKTEDEIALEKDSVVKNFIPHYIKDSSAIEKYKYLMQTKIEEI
                     LDSLKTRSNKIFILSTTRKQINTLENFYKEFETVNATYYNRGIIELSEFDYNNKLEQV
                     YFDKNGTSELTSVNNFYTKSEFSKALLDIYDKCMLETLDSVWCVEIQNFISPDYIPAN
                     IVYNQELNEKEINEEIDNLSPILGVIQAGQVIVRKGNIIGDYECKVLNSMQNYMTKDL
                     QHNTWSVSLGILLVFVLLFSVLFVYFLNFSCEIMNSFKTSSFFSIQILIMILTVYLVF
                     QFTDFSINNIPFVLFPLLIFTFYKFRVSFFVYLFTLFIVGFFAPSQFEFLIIQTLAGL
                     AAMFSLRKSQKRQHIFVTVLIVFGVYVVLNLGFSLMKGKGLGLEFINELKPFAVSSFL
                     VLLYLPFVYILEKAFGLVSDYTLLELSDINHPALRLLSEQAPGTFQHSMQVSNLAESV
                     VNALGGNSILTRVGALYHDIGKISHAEYFIENQSGVNIHNELDFEESATKIISHVKEG
                     MLIAKKYNLPSQVADFIETHHGTSLTKYFYVSWVNANPGLEPNMLNFKYPGPKPHSIE
                     TAVLMMADAIEAASRTLQDYTVENIQNTVSNIIDSQLNDGQFNEVSITLEQITKAKKI
                     FAEKIKNIYHARIVYPEINSKN"
     gene            3699..6167
                     /locus_tag="PKN53_05505"
     CDS             3699..6167
                     /locus_tag="PKN53_05505"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOD88439.1"
                     /translation="MKLKIIFNIIALFLVSSFSFSQTATLTGIVHDSKSKALHYANIS
                     VVGHSIGTTSDKKGYFELIVPASIDINIEVSYLSFETKTFLLNLNPGEVFNLEVELYE
                     AANILPSTDIVASSDRHGTAVRLNPEISLKIPTIGGFEDMLKSLPSVSSNNELSSQYS
                     VRGGNFDENLVFVNDIEIFRPILVRSSQQEGMSFINSDMVSGVVFSAGGFEAQYGDKM
                     SSVLDIKYRKPSQFGGSLSASLLGTNVHVEGVSKNHLFRYNTGLRYKTTKYVLGTLDT
                     EGNYDPQFFDFQTYCTYDVSDKLELSFLGNASSNVFNFIPEDRATSWGTLNEALKIMM
                     YFEGQEKNKFQNTTGALTAKYKKNKNLDMKFILSSYYALEQEAFDILGQYYLNELDKQ
                     LGSDNLGDSVANIGIGSYLNHARNYLNAYVTSFKYLGNYKTGNHSILWGAQYNYETFD
                     YSVHEWNMIDSAGYSLGSYGYSQQELIPENRDIVPLFLTDIDNMKIYSNRLNAFIQDS
                     YLIKLDAGEFSMGGGLRFNYWSFNHEFLISPRLNMGFKPNCKRDVVFRFSTGLYHQPA
                     FFKEIRRLDGSINRDIKAQSSYQIVAGGDYIFKAWGRPFKLVTEAYYKYMYNLIPYDI
                     DNVRIKYYGDNLSRGYSMGFEAKINGEFVPGTESWFSVAVMQTQEDIEGDFYRQKYAD
                     GTVDTIYLDMIPRPTDQRLNFGIFFQDYIPKHETWQAYINLLFGTGLPVEKAKEPARF
                     KAYRRIDLGISKQLLDGESQFATSRKFWSNFKSIWLSLEVFNLIDIKNEISYTFVTDI
                     RGWQYGVPNYLTGRRFNLKLMVKF"
     gene            6282..7556
                     /locus_tag="PKN53_05510"
     CDS             6282..7556
                     /locus_tag="PKN53_05510"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HOD88440.1"
                     /translation="MKKQKKYYTFFDAFIINIFTFLAVLLLWAIIDSVDLFNPLDEFF
                     DNFDYTDLYYSELRHNDMVPDTNIFIVNIGTLSRAELAKQITVLQKFQPKVIGIDAVF
                     AEQKGMEDYLLKQALNLDNNIVLGGFGEFDEDDGDEAIGIIKSNPFFGEFPVGHLEFI
                     ADPKIVREFDKFIRFNDTIINAFSLEIMSKYDPNVFLEFAQRKSKTEIINYRAGFHPY
                     IIFDYEEISDTNSNLEIIKDKIVLMGYVRMHQGAPTDTIDSHFTPIKRSEYGYADTKG
                     IEIHAHILSMLLSKDYVQKVPDWINYLIALIICQLFLMWLVYYYVHGAKLFDILSKPI
                     QFLLIVLVLWATFMIFAKFGIKIDMIPSVLTLVLCIEVLYLYEETLELFKIDSYLTQN
                     FKYTRDERIENFRYIVRLEFIRNRFKRTRKRV"
     gene            7510..8277
                     /locus_tag="PKN53_05515"
     CDS             7510..8277
                     /locus_tag="PKN53_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88441.1"
                     /translation="MSLFAIGLNAQESEYKVFYFSGSPKIVEKGKEISVKRDTYLNSK
                     ASIKLPANSYLVLTNKNEVPMGISNPGTYSIADLNKIYENVGNSNLTQEFFDYIANNM
                     IQENEKARRSGGVYRAVGDILVAPFDEAVLLIDSLTLSWKNPKQKKLYLKVYNPGNWE
                     LIYNIPTTDSTYTLYYEPQKFIKGREYAWTVYHGEDHPQQGTILRVFTFADDTWKENF
                     NNKLKVIKESGENVEINKIKTLRLYLDNNIYPLSESE"
     gene            complement(8286..9062)
                     /locus_tag="PKN53_05520"
     CDS             complement(8286..9062)
                     /locus_tag="PKN53_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOD88442.1"
                     /translation="MSKLKVSVLQTDIIWENAEENLKNYNKLILQNPGSDLYVLPEMF
                     NSGFTNNVYGCAEDNNGKSLTTLSEISRITGAAIATSLILKENDLFYNVFVFVKPDDR
                     IIKYNKRHLFKLGGEGKMFTKGSERVVFEFRGFKILFQVCYDLRFPVWSRNTEGYDLM
                     INVASWPIERRKMFDILLRARAIENLCYVVGCNRIGIDGNNLFYTGGSCIIDPKGEYI
                     ALANDEQEEIITATLDTDTIQEYRKSFPALDDKDDFNILV"
     gene            complement(9055..9606)
                     /locus_tag="PKN53_05525"
     CDS             complement(9055..9606)
                     /locus_tag="PKN53_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOD88443.1"
                     /translation="MKKTFAKIYFKLNRWKYNEFPDIKKAVVLMAPHTSFWDFVLGKL
                     YFNLYNYKPQILIKKEAFWGPLGYFLKKWGGIPVDRGKRNGIADMLTKRIKEAKDRFY
                     LVITPEGTRKKTKNWKKGFIKIAKEADVPVIVGFIDKKTKTLGVIGLLDMQGTDMEIM
                     ERLKKHYVGITGLRKNKFETGYE"
     gene            complement(9587..10204)
                     /locus_tag="PKN53_05530"
     CDS             complement(9587..10204)
                     /locus_tag="PKN53_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009228478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOD88444.1"
                     /translation="METDYFYTIASPSEGIFTEKSSKFLAFAFQVSSEEQVKQHLNDL
                     KKKYYDARHHVYAFIIGTKQETFRASDDGEPSNSSGPPVLGQIRSLNLTNTLIVVVRY
                     FGGTKLGIPGLINAYKTAAKNALDNAKIIKKYIEEKIEITFDYEDMNFVMKVIKDYEA
                     NIISQEFQENCSIICTIRLSLYENFLESLKQNHKLIIKLDEEDIC"
     gene            complement(10314..11741)
                     /gene="dnaA"
                     /locus_tag="PKN53_05535"
     CDS             complement(10314..11741)
                     /gene="dnaA"
                     /locus_tag="PKN53_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOD88445.1"
                     /translation="MVKKCEEIWGKCLQIIKDNVPKVSYQTWFEPIVPLDINDKVLAI
                     QVPSEFFYEFIEENFLDLLKKTLNKVIGTGAKLEYHVVIENGASADKTYVLKKPSSDK
                     IPITNRPVQVPLDLEANAIVNPFIIPGIKKMHVDPNLNLEYTFDNFVEGECNRLGRSA
                     GYAVAQNPGGTAFNPLFIYGNSGLGKTHLAQAIGIEVKKIMPEKVVLYVDANKFLTQY
                     MEATRNNNRNDFIHFYQMIDVLIVDDVQYFSKKEGTQEVFFHIFNHLHQHGKQLVLTS
                     DKPPVELNGMEQRLLSRFKWGLSADLQEPDFETRIKILKKKTYKDGIDIKEEILEYIA
                     SHITNNIRELEGALVSLLAQSTLNKKEITLELAKDMIDKLVKSTRREVSIDYIQKVVC
                     NYFSMPVETLSSKTRKREIVQARQIAMYFAKTMTKSSLAAIGAAIGNKDHATVLHAFK
                     TVNNLIDTDKKFKADVEEIEKRLKM"
     gene            12220..12798
                     /locus_tag="PKN53_05540"
     CDS             12220..12798
                     /locus_tag="PKN53_05540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOD88446.1"
                     /translation="MENRLCVFCSSSNDLEPKYYKLAEDLVLEIKKANYSIMHGAGSI
                     GLMGSLLNAAKENNVNIIGVVPEKLNKPHIVDSKNQKLVVTKDMKDRKEYLRENSSAF
                     IALPGGFGTLEEILEVITLKQLKYHNKAIVFINAYGYFDNLFKQFDYMFETNFANHNY
                     RNLYFVANSPSEAIDYINNYTHQNIYDKYLRE"
     gene            12814..13926
                     /locus_tag="PKN53_05545"
     CDS             12814..13926
                     /locus_tag="PKN53_05545"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOD88447.1"
                     /translation="MRNPFPEEFYKQRTKPWTPDVRGDFFRDQTAIIHSTVFRRLKNK
                     TQVFFAPENDHICTRIEHVLHVATIAASICKGLNQYSHNLDVEMAYAIGLGHDLGHTP
                     FGHAGEKALNKILGGNNAFIHEINSYRQAEYLANKGKGLNLSYGVKDGIICHNGEKDE
                     QYLKPTKTPNVLDEIKDRRVVATSYEGCVMRFADKIAYLGRDIEDAFAAGFISEKDIP
                     ADVKEALGNRNGQIINNLIIDIVENSKDSEKIGFSDRTYEFVTKLKHFNYKYIYSHHK
                     LQTYDLFCDKIIRTLFDYLMNLMDKNAYEFQKYSESAIDLDRYFGNYLKDMFEFYNKT
                     KDTNSQIVTDYIAGMTDVFTLEAIKQITIPKPIHFI"
     gene            13926..14453
                     /locus_tag="PKN53_05550"
     CDS             13926..14453
                     /locus_tag="PKN53_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88448.1"
                     /translation="MKILNHEILTIDFSSNSDYDSFFDIITNKINSIEDIEKPYLKKI
                     IICAIELIQNNIIHNCKEVSRLEIFKKDNFIILDYVQSTDKQIFENLVKHIKFVNELN
                     FENISLKIKGNIENTKSNIGSGNGLLTCRLKTKNFVNIKLLEAKSEDEYKFIIRLKID
                     YYENTNSSSSKNTIG"
     gene            14410..14766
                     /locus_tag="PKN53_05555"
     CDS             14410..14766
                     /locus_tag="PKN53_05555"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOD88449.1"
                     /translation="MKTQIPAQAKTPLVEKDLELNIIKIVGVLIPENPMLFFEELYSF
                     TEEFLQKTKKLNLHVDLEYFNTSSARYLYDYLKKIIPISDLQLTWFYDSDDEDILESG
                     KEFEELCKIKFEFVEK"
     gene            complement(14790..16130)
                     /gene="trkA"
                     /locus_tag="PKN53_05560"
     CDS             complement(14790..16130)
                     /gene="trkA"
                     /locus_tag="PKN53_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOD88450.1"
                     /translation="MQIVISGAGEIGLYLAKMLVKEKHDVSLIDINDTIIKEVDVSSY
                     DLMTVCGDCTNFSVLKSVQTHTSDLYIAVTNSQDTNILSCIFAKKLGAKKTIARVNNM
                     EYLNPINKLSFINLGIDKMIYPELLAAKEVVGVIKQTGTTEIFEFSGGKLTLFVIKLD
                     KNSPILNLNLMETSQLVHNKHFRAVAIKRLDETIIPKGKDILKEDDSVYIISTPDGVK
                     ELLKIFGKERHDINNVIFLGGSRISIKASKYISQQLKVKIIESDPDKCLKLVEMLPNT
                     MIINGNGRNTDLLLEAGLEKCDAFVAVTGDSETNLITCILAKRYGVKQTFAEIENLDY
                     IDIASKMGIDTIINKKLIAASNIYTQTMKAEVTSIKCLTGTDAEVLEFVASKNAIITQ
                     NRLKDINFPEGVIIGGIVRGKKSFIAMGDTQIKANDRVVLFALPEAIHKIEYYF"
     gene            complement(16146..17042)
                     /locus_tag="PKN53_05565"
     CDS             complement(16146..17042)
                     /locus_tag="PKN53_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOD88451.1"
                     /translation="MDKNKIISLLKLLEDPDEKVFSIVKDEIVGHGELFKAYLENYHA
                     LSTNSLALERSEDILDEIFWESFETKLIEYFTNPEAKFYEGVFLIEKFFNRDIDTKEL
                     QTDYSILKTSIWIEMSNQLTNIEKINVLNTTLFDKLGYTKLTVKEIKSSTLSITYCIS
                     NKKFLPPNIAVLYCMLADEIQIPVFPINLPELFALCYRNADIHSEVFKNKSNDIIFFL
                     FPSEKGAIISKDLANRHLERLKSKSQIKIDTTIDDIEATSYDNLLLNYFNIRIKSLKI
                     SNTDNFCTKYAKKVEDIFHEYL"
     gene            complement(17047..19245)
                     /locus_tag="PKN53_05570"
     CDS             complement(17047..19245)
                     /locus_tag="PKN53_05570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOD88452.1"
                     /translation="MGLTKEEEIWIESQFDELIKVSKRLRSQDDIDLVRKAFDLANEA
                     HSKMRRKSGEPYFTHPIAVAMIVASEIGLGVKSIVSALLHDVVEDTDYTLNDIETLFG
                     PSIAGIVGGLTKIANILDKDASLQVENFRRMILTMSQDIRVIFIKLADRLHNMRTLDS
                     MPEYKQIKIASETTYLYAPLAHRLGLYSIKTELEDLALKYEHPLIYWEISKKFTETEE
                     ERNKYIEQIKLPIQKVLEEENIKANIIGRTKSIYSIWNKMRTKNVPFEEIYDLFAIRI
                     IFDHPDDYPDSKARAKCYHIMALVTNIYPHLEDRVRDWIAKPKANGYEALHVTVMGPN
                     GDWIEVQIRSRKMNDIAEKGLASHWKYKANNKDALGNLDNLINTLQQTLENNVNDTLS
                     FLDDVKMNIFTKEVYVFTPKGEIKKIPSGATVIDFAYEIHTEVGNKAIAAKVNHQLKP
                     LSHVLKSGDQVEILTSDKQSPKREWLNFAITTKAKNRIKSSLKEDRRYHIDKGQEIFI
                     KKVRELNLKLDHKRFKELRKTYNTPHKEDFYFKIGSGLIQIDDLEKQAAKTFKTFASY
                     WKLSIPKFRTSQPKSKKNTLIIDDNADTKRITLAKCCNPIPGDEVAGFVNPNESIIIH
                     KRICERLTNLAATQGDRIISVKWTSYKLQSFKAKIQIEGMDKLGMLNKITKVISLDSR
                     VNIKTLSFNTDDEIFSGEIILYVHNLQDLEDIMKDLLKIDGVEKVTRLEE"
     gene            complement(19306..20820)
                     /locus_tag="PKN53_05575"
     CDS             complement(19306..20820)
                     /locus_tag="PKN53_05575"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOD88453.1"
                     /translation="MIKRIFFLIILSLAISNIGLGQIDIPDAPKFISASIIPESNPTS
                     VHIKWLPSDSLDVQGYIVYQIINSITETIDTVWGRETTEYIYGLGNYQQSEKFRLVSF
                     DNQYYKSNITSPHTTIYLAHEYNKCNKYVSLNWTNYEGWQTVKEYNIYRRNIEGTYER
                     ITKVSGDKTSYEDKSLEANNTYYYYIEAVSPENYKASSNSIKVKTEAYRAPNFLITSQ
                     ASVKDNKIDLKFVVDNTAEIIEFRIQRATSPDGDYKTIKVVQNTSQDVILISDNEIDT
                     NNEIYYYRIASVNPCGDINAYSNYACNILLKAETAEDLNHYLSWNEYQQWANGVFNYK
                     IYRIFDNIEAEIDVNPNNLFDYTYNIEWYVKYCHNLKINTSSKYCYYVEAVEKQGEIA
                     IISKGRSRSNIACVYHEPVFWIPNAFNVSSIEPENKVFKPIISFIKEEPYEFSVYNRF
                     GMKVFETNDSQEAWDGKINKTYTAPSQYYTYYVKYFDFNDKQHIKTGIFFLFIK"
     gene            complement(20917..21531)
                     /locus_tag="PKN53_05580"
     CDS             complement(20917..21531)
                     /locus_tag="PKN53_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88454.1"
                     /translation="MEYIDIIIVIASLTLTAGFICNRKAFKQHLIFISLIAIVVYSFL
                     RPFYPIGIVAALGIISHIIFIIKKSKTKSTIKLLEVEFDNAYIQEFINNYKRDIYSFF
                     PFYQAEKGQRCFLMMLNADMAGILIAKINKNEMLIEVDYTKPDYRNEVIGKYIFKQNT
                     GYFKKLGIDKLLTKSYHKGHSKYLKKMGFEQVNLDNQSFFVKNL"
     gene            complement(21637..>23310)
                     /locus_tag="PKN53_05585"
     CDS             complement(21637..>23310)
                     /locus_tag="PKN53_05585"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HOD88455.1"
                     /translation="GTTWIPNTFNVPENLSPGDISAIDDMNAWVALYDGTNGGGGIYR
                     TSDGGETWIHQASAAFNNSSSFCNGVHMFNANDGYCIGDPVNGEFELYLTNDGGENWT
                     LIDGNNIPDPQSNEMGWTGVNCAIGNNAWFGTSTGRVYRSTDKGQTWTAHATGQSNVS
                     KISFADENNGVAICATYNQTTGTIQSWKLIRTTDGGETWEIIPVEDQYISDISAVPGK
                     PGMYVGVKISQTIEDNFSAYSLDYGTTWEMLDDSVQYTNVKMFNQNTGWAGGFNLDAN
                     TNGIYKWLGIPNLDSPYFTSLPITSINEGEEYSYEITAEDPNTLPLTITAQEKPDWLE
                     LTDNGNGTAILTGTAPTITQISQNFNVTLQASNGTDIGEQVFTITVHTTNTPPEFVSE
                     PITSGVQYELYEYNIEATDADGDVITLTATTLPDWAEIIDNGDGTGKITGTPTEKSVI
                     GFQVKIQASDGIHQVEQSFRVKVAESYVEDFGHGKINIFPNPTKNILNIQNCQNTNYE
                     IIDVTGKVLQTGKINDSIETLDMSNFAKGNIFIKLQDNENVYIMKIVKM"
BASE COUNT         7684 a         3300 c         3565 g         8761 t
ORIGIN      
        1 agatttacca gaaggatatg gaaactccac tagtatatat ttcttaggct tttctttggc
       61 tttttctttg gctttttcta atataaatcc ctcttctaaa tatttttcag aaacattttc
      121 tattactcta aaagcagtta ttaatgaaaa atcgaatttt gctttaccat ttccattttc
      181 aattaattct ttttgtactc tatctacacc acgactaaat ctatttacgt agcctaacac
      241 tttcatagct tcggcgatga acggattacg gtagtcgctg acatttggga agttttctgg
      301 agttactttt ccgtatagtc caccgggatt ttgtacttca attctgtcgt catattcgta
      361 gaattggaca ggagcgttgg tttcgtaatc gcggtgcata attgcgttca ttaaaagttc
      421 gcgagtagcc cagtatggat attttgaaaa agtttcttca cgaagtacgc ttactggaat
      481 aggacgtttt tgtgctatgc tggtttcgat aaacgtatca attttgggaa gctcccgaca
      541 aagatttcct gcgaatttat gctcattaat aatatctcca gcacggtctt ttcctttgaa
      601 atgtacatat tgaatatatg agccgtgtag gtagcgttct ggattttttc caaaaagtac
      661 aatagctcca ttagttggac agttgtaacg caaatcataa aatccaagcg aagcaagttg
      721 ttcttctata caacgattgt cttcggcgag tatatcttca gctactgctt taggtaaata
      781 ttctttttct ataattttta cgtctaaatc gttgatgttt gttcctaagc aaggcatggc
      841 atcgaatgtg tgaatgttgg ataaacggcg ttccattagg attttttctt ccgcttctgt
      901 tgctgtgctt tttcttggac caactcttac ccaaatacgt cctctataac gcacaggagg
      961 aaattctgag ggttgaactt cgacaaccaa tagctcgcca tcttcaaaag agaatttttc
     1021 tacactcata gtcggctgag gtaaaatatt gccatcggtg cgaatgttgg atatctttaa
     1081 taataactta tcgtcaactt ttaagcctga taatttcccg tcattgtgtg ctccaataat
     1141 taaatagcca ttctttccac ttgacgaaac atcgttggaa aaggaacaaa tagcttgaca
     1201 aaatttatcc atatcgttgg tagatacgat gcgttctata tgggaacttt ccgaagcaga
     1261 aagaattttt gaaagactat tttttgtgag cataaactgt attttatgca aaaataaagt
     1321 ttttctaaac ttttagcaaa caatttacta taaattaaaa tgcaaaatgc tattcatttg
     1381 atatatgctt attaagtaaa gttgaaatgt cttttattta aaccaaaaaa atattcaaat
     1441 tttcttaatt atcacttcat tttaaaatct ttattcattt ttaattttaa aatattatat
     1501 ttgcttttgt ttaattcata aaatgaacaa atgagtaaaa gaaaaattga aaataagggt
     1561 gtttttattt atttagttat tctttttgtt ataagcgtaa gtttggttgt atttattttt
     1621 ccacgaaaag aacaatttca atactcttat gaaccgggca caccttggct ttatgaaagt
     1681 ttagtggcag aaactgattt tcctatttat aaaactgaag atgaaattgc tttagaaaaa
     1741 gatagtgtag taaaaaattt tataccacat tatattaaag atagttctgc aattgaaaaa
     1801 tataaatatt taatgcaaac taaaatagaa gaaatattag attcgctaaa aacacgtagc
     1861 aataaaattt ttatattgag taccacaaga aagcaaatca atacgcttga aaacttttat
     1921 aaagagtttg aaacagtaaa tgcaacatat tataatcgtg gaataattga actttctgaa
     1981 tttgattata acaataagtt agaacaagtt tattttgata aaaatggaac ttcagaattg
     2041 acttctgtta ataattttta cacaaaatca gaatttagta aagctttgtt agatatttat
     2101 gataaatgta tgcttgaaac acttgattcg gtttggtgtg ttgagattca aaattttatt
     2161 tcacctgatt atatcccagc aaacatagtg tataatcaag aattgaatga gaaagaaatt
     2221 aatgaagaaa tagataattt aagtccaatt ttaggtgtaa ttcaagcagg acaagtaatt
     2281 gttcgtaaag gaaatattat aggcgactat gaatgtaaag tgttaaattc tatgcaaaac
     2341 tatatgacaa aagatttgca gcacaatact tggagtgtta gtttgggaat tttgttggtt
     2401 tttgttttac tattttcagt attgttcgtt tattttctga atttttcttg cgaaataatg
     2461 aattcgttta aaaccagtag ttttttctca attcagatat taataatgat tttaacggtt
     2521 tatttagttt tccaatttac tgattttagc ataaacaata ttccttttgt actttttccg
     2581 ctactaatat ttaccttcta taaatttaga gtttcatttt ttgtttactt atttacatta
     2641 tttatagttg gattttttgc accatctcaa tttgaatttt taattattca aactttagct
     2701 ggtttagcag ccatgtttag tttgagaaaa agccaaaaac gacaacatat ttttgtaact
     2761 gttcttatag tttttggagt ttatgttgtg ttaaatttgg gcttttcatt aatgaaagga
     2821 aaaggacttg gtcttgagtt tataaatgag ttaaaaccat ttgcagtaag ctcgttttta
     2881 gttcttttat atttaccttt tgtttatatt ttagaaaaag cttttggttt agtctcagac
     2941 tatacattat tagaattgag cgatattaat catcctgctt tgagattgct ctcagaacaa
     3001 gcaccaggca cttttcaaca ttccatgcaa gtttctaatt tagcagaatc tgttgtaaat
     3061 gctttgggag gaaactctat acttacaagg gtaggggctt tgtatcatga tattgggaaa
     3121 ataagtcatg ccgaatattt tatcgaaaat caatcaggag ttaacataca taatgaattg
     3181 gattttgaag aaagtgccac aaaaataatt tctcatgtta aagaaggtat gctaattgca
     3241 aaaaaatata acttaccatc tcaagtagca gattttatcg aaactcatca tggtacgtct
     3301 ttaacaaaat atttttatgt ttcatgggtt aatgcaaatc ctggactaga gccaaatatg
     3361 ttaaatttta aatatcctgg tcctaaacca catagcatag aaactgctgt tctgatgatg
     3421 gctgatgcaa tagaagctgc ttcgagaact ttgcaagatt atactgtcga aaatattcaa
     3481 aatacagttt ctaacattat tgattctcaa ctcaatgatg gtcagtttaa tgaagtaagt
     3541 attacattag aacaaattac taaagctaag aaaatatttg ctgaaaaaat aaaaaatatt
     3601 tatcatgctc gcatagttta tcccgaaatt aattcaaaaa attaattaaa aacaatatat
     3661 ttgcggactt ttagttataa ctatgttttc taagctttat gaaactgaaa ataatattta
     3721 acatcatagc tttattttta gttagtagtt tttcattttc tcaaactgct acgctaactg
     3781 gcatagtaca tgactcaaaa tcaaaggctt tgcactatgc aaacattagc gttgtaggac
     3841 attcaatagg aacaactagc gataaaaaag gatactttga attaatagtg ccagcttcta
     3901 tcgatataaa tattgaagtt tcttatttaa gttttgaaac aaaaacattt ttactaaact
     3961 taaatcctgg cgaagttttt aatcttgaag tagaattata tgaagctgca aatattttgc
     4021 caagtacaga cattgttgca agttcggata ggcatggaac agcagtaaga ttaaatcccg
     4081 aaatttctct taaaattcct actattggcg gttttgaaga tatgttaaaa tctttgcctt
     4141 cggtttcgtc taacaacgaa cttagttcgc aatatagcgt tagaggtgga aattttgatg
     4201 aaaatttagt ttttgtaaat gatatagaaa tttttagacc tattctagtc agatcgagtc
     4261 aacaagaagg aatgagtttt attaattctg atatggtttc gggtgttgtg ttttcagctg
     4321 gagggtttga ggcacaatat ggagacaaaa tgtcatctgt gcttgatata aaatatagaa
     4381 aaccttcgca atttggagga agtttaagtg ctagcttatt aggaacaaat gttcatgtcg
     4441 aaggtgtaag caaaaatcac ctttttagat ataatacagg tctcagatat aaaacaacaa
     4501 aatatgtttt aggaacttta gatacagaag gaaattatga tcctcaattt tttgactttc
     4561 aaacatattg tacttatgat gtaagcgata aactcgaatt aagttttttg ggaaatgcaa
     4621 gcagtaatgt ttttaatttt attccagaag atagagctac ttcttgggga actttaaacg
     4681 aagctttaaa aataatgatg tatttcgaag gacaagaaaa aaataaattt caaaatacta
     4741 caggagcttt aacagcaaaa tataagaaaa ataaaaattt agatatgaag tttattttgt
     4801 cttcgtatta cgcattagag caagaagctt tcgatatttt aggtcaatat tatttgaatg
     4861 aattagacaa acaattaggt tcagataact tgggcgacag cgtagcaaac ataggtatag
     4921 gttcttatct aaatcatgcc aggaattatt tgaatgctta tgttacttca ttcaaatatt
     4981 taggaaatta taaaacaggg aatcacagta ttttatgggg agctcaatat aattacgaaa
     5041 cctttgatta ttctgtccat gaatggaata tgatagattc ggcgggatac tctttaggat
     5101 cttatggata ttcgcaacaa gaactaattc ccgaaaatcg tgatattgtg cctttgttcc
     5161 taaccgatat tgataatatg aaaatttata gcaacaggtt aaatgcattt attcaagatt
     5221 cttatttaat aaaacttgat gcaggagaat tttctatggg tggaggatta agatttaatt
     5281 attggagctt taaccacgaa tttttgataa gtccacgttt gaatatggga tttaaaccaa
     5341 attgtaaaag agatgttgtt tttagatttt ctacaggatt gtatcatcag ccagcgtttt
     5401 ttaaggaaat acgaagatta gacggaagta taaatagaga cattaaagca caatcttcgt
     5461 atcaaattgt tgcaggagga gattatattt ttaaagcttg gggacgacca tttaaacttg
     5521 ttacagaagc atattataaa tatatgtata atttaattcc ttacgatatt gataatgtga
     5581 ggattaaata ttatggggat aatttatctc gaggttatag tatgggattt gaagcaaaaa
     5641 ttaatggaga atttgttcca ggaacagaat cttggtttag cgttgcagta atgcaaactc
     5701 aagaagatat agaaggggat ttttataggc aaaaatatgc tgatggaaca gttgatacta
     5761 tttatttaga tatgatacct cgtccaaccg accaaagact taatttcgga atatttttcc
     5821 aagattatat tcccaagcat gaaacatggc aagcttatat aaatttattg tttggaacag
     5881 gtttaccagt tgaaaaagct aaagaaccag ccagatttaa agcatatcgt agaatagact
     5941 taggtatttc aaaacaattg ttagatggtg aaagtcagtt tgcaacatca aggaaatttt
     6001 ggtctaattt taaatctatt tggttgagcc ttgaagtgtt taatttaata gatattaaaa
     6061 atgaaatttc atacactttt gtaaccgaca ttcgtggttg gcaatatgga gttccaaatt
     6121 acttgacagg acgaagattt aatcttaaat taatggtgaa gttttaaaaa cattttctga
     6181 aagttaagtt tattttagtg aaaaatattt ttattgaagt attgtttttt tctaaaatta
     6241 tatatatttg tcataaaata tgtgagaatt aatttaagca catgaaaaaa caaaaaaaat
     6301 attatacttt ttttgatgct tttataataa atatatttac atttttagca gttttgcttt
     6361 tatgggctat cattgattct gttgatttgt ttaatccctt agatgaattt tttgataatt
     6421 ttgattatac cgacttatat tattcagaat taagacataa tgacatggtt ccagacacta
     6481 atatttttat tgtaaatatt ggaactttaa gtcgagcaga attagctaaa caaattacag
     6541 tccttcaaaa atttcaacct aaagtaatag gaatagatgc ggtttttgcc gaacaaaaag
     6601 gaatggaaga ttatttgtta aaacaggctt tgaatttaga taataatatt gtattaggag
     6661 gatttggaga gtttgacgaa gatgatggag acgaagctat tggtataata aaatcaaatc
     6721 ctttttttgg cgagtttcca gtaggacatt tagagtttat tgccgaccca aaaattgtta
     6781 gagaatttga caagtttatt agatttaatg atactataat taatgctttt tctttagaga
     6841 taatgagtaa atatgaccca aatgtatttt tagaatttgc tcaacgtaag tcaaaaactg
     6901 aaattattaa ttatagagcg ggttttcatc catatataat ttttgattat gaagaaattt
     6961 ctgatacaaa ttcaaattta gaaattataa aagataaaat tgtgctaatg ggctatgtaa
     7021 gaatgcatca gggagcacct acagatacta ttgatagcca ctttacacca ataaaacgtt
     7081 cagaatatgg ctatgcagat acaaaaggaa tagaaattca tgcacatatt ttaagtatgt
     7141 tgctttcaaa agattatgtt caaaaagtgc cagattggat aaattactta atagctttaa
     7201 ttatatgcca attgttttta atgtggttgg tttattatta tgtacatggg gctaaattat
     7261 ttgatatatt atcaaaacca atacaatttt tattaattgt tttagtttta tgggcaactt
     7321 ttatgatttt tgcaaagttt ggtataaaaa ttgatatgat accatcagta ttaactttgg
     7381 ttttatgtat agaagtgcta tatttatacg aagaaacgtt agaacttttt aaaattgatt
     7441 catatttaac acaaaacttt aaatacacta gagatgaaag aattgaaaat tttcgttaca
     7501 ttgttcgcct tgagtttatt cgcaataggt ttaaacgcac aagaaagcga gtataaggta
     7561 ttctattttt caggcagtcc taagattgtt gaaaaaggta aagaaataag tgtcaaacga
     7621 gatacttatt taaactctaa agcaagtatt aaacttccgg caaattcata tttagtatta
     7681 actaacaaaa atgaggtgcc tatgggtatt agtaatcctg gaacttatag tattgctgat
     7741 ttaaacaaaa tttatgaaaa tgttggtaac agtaatttaa cacaagaatt ttttgattat
     7801 attgctaata atatgattca agaaaatgag aaagcaaggc gtagtggtgg tgtttataga
     7861 gctgttggcg atattcttgt agctccattt gatgaagctg ttttattaat tgatagtctt
     7921 actttaagtt ggaaaaatcc aaaacaaaag aagttgtatc taaaggttta taatccagga
     7981 aattgggaat taatttataa tattccaaca acagattcaa cttatacttt gtattatgaa
     8041 cctcaaaagt ttattaaagg cagagaatat gcatggactg tttatcatgg tgaagaccat
     8101 ccacaacaag gaacaattct tagagttttt acatttgccg atgacacatg gaaagaaaac
     8161 tttaataata aattaaaggt aattaaagaa agtggtgaaa atgtagaaat taataagata
     8221 aaaactttaa gactatattt ggataataat atttatcctt taagcgaaag cgaataagct
     8281 tttgtttata ctagaatatt aaaatcatct ttatcgtcta aagcagggaa acttttacga
     8341 tattcttgaa tagtgtctgt atcaagagtt gcggttatta tttcttcttg ttcgtcgttt
     8401 gctaatgcaa tatattcgcc tttagggtca attatgcatg aaccacctgt gtagaacaag
     8461 ttattcccat ctataccaat tctgttgcag cccacaacat aacaaaggtt ttctattgct
     8521 ctagccctta gaagtatgtc aaacatttta cgtctttcta tgggccatga tgcaacattt
     8581 atcattaagt cgtagccctc agtatttcta ctccatacag gaaagcgtaa gtcataacac
     8641 acttgaaata atattttgaa tcctctaaat tcaaaaacaa ctctttcgct acctttggta
     8701 aacatttttc cttcgccacc aagtttaaac aaatgtcttt tattgtattt tataattcta
     8761 tcatcaggtt ttacaaaaac gaaaacattg taaaacaaat cattttcttt taatattagc
     8821 gaagttgcta tagcagcacc cgtaattctt gatatttcac taagtgttgt taggcttttc
     8881 ccattgttgt cttcggcaca gccatataca ttgtttgtaa agccagaatt aaacatttct
     8941 ggtaagacat ataagtcaga gccaggattt tgtaatatta acttattata attttttaag
     9001 ttttcttctg cattttccca aataatatcg gtttgtaaaa ccgaaacttt taatttactc
     9061 ataacctgtt tcaaatttat tttttctaag tccagtaata cctacataat gtttttttaa
     9121 tctttccata atttccatgt cagtgccttg catgtctaat aaacctatta cgcccaatgt
     9181 tttggttttt ttatctataa aacctacaat aactggaaca tcagcttctt tagctatctt
     9241 tataaaacct tttttccagt ttttcgtttt ttttcttgtt ccttctggtg taattacgag
     9301 gtaaaatctg tctttggctt cttttattct ttttgtaagc atatctgcta tgccgtttct
     9361 ttttcctctg tcaacaggaa ttccgcccca tttttttaaa aaatatccta aaggtcccca
     9421 aaaagcttct ttttttatta aaatttgcgg tttgtaatta tataagttga aatataattt
     9481 tcctaaaaca aaatcccaga agcttgtgtg tggagccatt aaaactacag cttttttaat
     9541 atcaggaaac tcattgtatt tccatctgtt taatttaaaa tatattttag caaatgtctt
     9601 cttcatctag ctttataatc aacttatgat tttgttttaa agattctaga aaattttcat
     9661 acaaagataa cctaattgta caaattatgc tacagttttc ttgaaattct tgcgatatta
     9721 tattagcttc ataatcttta ataactttca ttacaaagtt catatcttca taatcgaaag
     9781 taatttcaat tttttcttcg atatattttt tgataatttt tgcattatcc aaagcatttt
     9841 ttgctgctgt tttataagcg tttattaagc ctggaatacc aagttttgtt ccaccaaaat
     9901 atctaactac aactataagt gtgtttgtta aattcaatga tcgaatttgt cccaatactg
     9961 gtggtccaga actatttgat ggttctccat cgtcagatgc tctaaaagtt tcttgctttg
    10021 tgccaataat aaatgcataa acatgatgtc gtgcatcata atattttttt tttaaatcgt
    10081 ttagatgttg ttttacttgt tcttctgaac taacttggaa agcaaaagct aaaaatttac
    10141 ttgatttttc tgtaaaaatt ccttcacttg ggcttgcaat agtataaaaa taatcagttt
    10201 ccattaatta aaaacgtgaa tacaaagtgt ttttttacaa actttatggt aaaaaagtaa
    10261 gttgtcttaa atgtgttcaa aatcaaaagt ttgacacatc taagacatag tttttacatt
    10321 tttaatcttt tttcaatttc ttcaacgtct gctttaaact ttttatctgt atctatcaga
    10381 ttattaacag ttttgaaagc atgtaatact gtagcgtggt ctttattacc aatagctgca
    10441 ccaatagctg ctaaagaaga ttttgtcatt gtttttgcaa aatacatagc tatttgtcta
    10501 gcttgtacta tttcgcgttt gcgtgttttt gagctaagtg tttcaacagg cattgaaaaa
    10561 tagttacaaa caactttttg tatatagtca atagagactt ctcttctagt actcttaact
    10621 agcttgtcta tcatatcttt agctaattct aaagtaattt cttttttgtt aagagttgat
    10681 tgtgccaata aagaaactaa agcaccttcg agttcgcgta tattgtttgt gatatgagag
    10741 gcaatgtatt caagaatttc ttctttaata tcaataccgt ctttatatgt ttttttcttt
    10801 aaaattttaa ttctagtttc aaaatctggt tcttgtaaat ctgcagataa gccccatttg
    10861 aatcttgaga gtaatctttg ttccatgccg tttagttcta ctggtggctt gtcagatgtt
    10921 aaaaccaatt gttttccatg ttgatgtagg tggttgaaaa tatggaagaa tacttcttga
    10981 gttccttctt ttttagagaa atattgcaca tcgtcaacta tcagaacatc aatcatttgg
    11041 tagaaatgaa taaaatcgtt tctattatta tttctagtag cttccatata ttgagttaaa
    11101 aacttgtttg cgtcaacata tagcactact ttttctggca ttattttttt aacttctatt
    11161 ccaatcgctt gtgctaggtg tgtttttcct aatccagaat ttccgtaaat aaataaaggg
    11221 ttaaaagctg ttccgccagg gttttgtgca acagcataac cagccgaacg acctaaacga
    11281 ttacattcac cttcaacaaa attatcaaaa gtatattcta ggtttagatt tggatctacg
    11341 tgcatttttt tgatgccagg aataataaaa ggatttacaa tagcattagc ttctaaatca
    11401 agtggcactt gcactgggcg atttgttatt ggaattttat cgctagaagg ttttttgaga
    11461 acataggttt tatcagcact agcaccattt tcgataacta catgatattc taatttagca
    11521 ccagttccta ttactttatt taaagttttt ttaagtaaat caaggaaatt ttcttcaata
    11581 aattcataaa aaaactcact tggaacctgt atggctaaaa ccttatcatt gatatctaaa
    11641 ggaacgatag gctcaaacca agtttgatag ctaactttcg gcacattgtc tttaatgatc
    11701 tgcaaacact ttccccatat ttcttcacac ttttttacca tttcattaaa tagcttttaa
    11761 ttaatttaac tttaataaat atatttaaag tagccttttt tttgctttta aaattataat
    11821 ttaaacttta tctcttcaaa tcaaaaatta gcaaaaaaat taacaaactt attgtcagag
    11881 agtttgctaa tttttgtttt tgattaagca atcaaaaaaa tgcccttcaa atcatctgct
    11941 aaaataagac atttttttgt caaacaaaac atttttatac atttttttga aaaaaataaa
    12001 aaaatatcta tttgtgtcat tatcagtcat taagcggtat taactataac attacttcca
    12061 ttatgcatgt gtagcgattt agttttcaag ttgttatatt tttttgataa agaaaatgtt
    12121 ttttgttatt aactttattg ataatgaaat gttaaaaaat atatgtagtt gaaaatactg
    12181 attagcacaa tttgtttaaa tttacaaaaa aatgatttta tggagaatag actttgtgtt
    12241 ttttgtagct ctagtaatga tttagaacca aaatactata aattagccga agacttagtt
    12301 ttagaaatta aaaaagcaaa ttatagtata atgcatggag ctggcagtat aggactaatg
    12361 gggagtttgt tgaatgccgc caaagaaaat aatgtaaaca taattggtgt agtgccagaa
    12421 aagttaaata agcctcatat tgtagattca aaaaatcaaa aattggttgt aactaaagat
    12481 atgaaggata gaaaagaata tttaagagaa aattcttctg cttttattgc attaccagga
    12541 ggttttggta ctttggagga aattcttgaa gtgataactt taaaacaatt aaaatatcat
    12601 aataaagcta ttgtttttat aaatgcatac ggatattttg ataatttatt taaacaattt
    12661 gattatatgt ttgaaactaa ttttgctaat cataactata gaaatcttta ttttgttgca
    12721 aattcaccaa gcgaggcaat agattatatt aataattata ctcatcaaaa tatttatgat
    12781 aaatatttaa gagaataaaa aacacatttt attatgagaa acccatttcc agaagaattt
    12841 tataaacaaa gaactaaacc ttggactcct gatgttagag gtgatttttt tagagatcaa
    12901 accgcaataa ttcattcaac agtttttaga agattaaaaa ataaaactca agtttttttc
    12961 gctcctgaaa atgaccatat ttgtactaga attgagcatg ttttacatgt tgctacaatt
    13021 gcagcaagta tttgtaaggg cttaaatcaa tattctcata atcttgatgt tgaaatggct
    13081 tatgcaattg gattaggaca cgatttgggg catacacctt ttggacatgc tggcgaaaaa
    13141 gctttaaata aaattttagg cgggaataat gcttttattc atgaaatcaa ttcttatcgt
    13201 caagcagaat atttagcaaa taaaggtaaa ggactaaatt tgtcttatgg agttaaagat
    13261 ggaataattt gtcataatgg ggaaaaagat gagcaatatc taaagcctac aaaaacacca
    13321 aatgtattag acgaaattaa agataggaga gtagtagcaa ctagttacga aggttgtgtg
    13381 atgagatttg ctgataaaat agcatatctt ggaagagata ttgaagatgc ttttgctgcc
    13441 ggatttataa gcgaaaaaga tattccagca gatgtgaaag aagcacttgg gaatagaaat
    13501 gggcaaatta tcaataattt gattattgat attgttgaaa attctaaaga ttctgaaaaa
    13561 attggatttt cagatcgaac ctatgaattt gttacaaaac tcaaacattt taattataaa
    13621 tatatttata gtcatcataa actgcaaacc tatgatttgt tttgtgataa aataataagg
    13681 actttgttcg attatttgat gaatttaatg gataaaaatg cttatgaatt tcaaaaatat
    13741 tccgaaagcg caattgattt agatagatat tttggaaatt atttgaaaga tatgtttgag
    13801 ttttacaata aaacaaaaga tactaattcc caaattgtaa cagattatat tgcaggaatg
    13861 acagatgttt ttaccttgga agcaataaaa caaataacta tccccaagcc tatacatttt
    13921 atttaatgaa aattttaaat cacgaaatat taacaataga ttttagttca aatagcgatt
    13981 acgactcgtt ttttgatatt attactaata aaattaattc tatcgaggat attgaaaaac
    14041 cttatcttaa aaaaataata atttgtgcaa tagaattaat ccaaaacaat ataatacata
    14101 attgtaagga agtttcgcga cttgaaatat ttaaaaaaga taactttatt attttagatt
    14161 atgttcaatc tacagataaa cagatatttg aaaacttagt taaacatata aaatttgtaa
    14221 atgaattgaa ctttgaaaat attagtttga aaattaaggg aaatattgaa aatactaaat
    14281 ctaatatagg aagtggtaat ggtctgttaa cttgcagatt aaaaactaaa aattttgtaa
    14341 atataaaatt gttagaagct aaatccgaag atgaatataa atttataata agattaaaaa
    14401 ttgattatta tgaaaacaca aattccagct caagcaaaaa caccattggt tgaaaaagac
    14461 ttggagttaa acataattaa aattgtgggt gttcttatcc cagaaaatcc aatgcttttt
    14521 tttgaagagc tttactcttt tactgaagaa tttttgcaaa aaacaaaaaa actaaattta
    14581 catgttgatt tagaatattt taacacaagt tcagctaggt atttatatga ttatttgaaa
    14641 aaaataatac caatcagtga tttacaactt acttggtttt atgattctga tgatgaagat
    14701 attttggaaa gtgggaaaga atttgaggaa ttatgcaaaa taaaatttga atttgttgaa
    14761 aagtaaaact gaattataag ctaattttgt tagaaataat attcgatttt atgtattgct
    14821 tcgggtagag caaaaagtac aactctgtca tttgctttaa tttgagtatc tcccatagca
    14881 ataaaacttt ttttgcctct aactataccg ccaataataa cgccttcagg gaaattaata
    14941 tctttaagtc ggttttgggt tataattgca ttttttgaag ctacaaactc taaaacttct
    15001 gcgtcagttc ctgttaagca ttttattgaa gtaacttctg ccttcatagt ttgagtataa
    15061 atattacttg cggcaataag ttttttattt ataattgtat ctatccccat ttttgaggct
    15121 atatctatat aatctaaatt ttctatttcg gcaaatgttt gttttactcc atatcttttt
    15181 gctaaaatac aagttataag atttgtttct gaatctccag taactgcaac aaaagcatca
    15241 catttttcca aaccagcttc taacaataga tcagtgtttc gtccatttcc attgattatc
    15301 attgtgtttg gtaacatttc aacaagtttt aagcatttgt cagggtcgga ttctataatt
    15361 tttactttta gttgttgaga aatgtatttt gaagctttta tactaattct actgcctcca
    15421 agaaaaataa cattattaat gtcatgtctt tctttaccaa aaatttttag taattcttta
    15481 actccatctg gcgttgagat tatatataca gaatcgtctt cttttaaaat atcttttccc
    15541 tttggaataa ttgtctcatc taaccgtttg attgctactg ctctaaagtg cttattatgc
    15601 acgagttgtg aggtttccat taggtttaag ttgagaatag gactgttttt gtcgagtttt
    15661 attacgaata gggtaagttt accacccgaa aattcaaaaa tttctgttgt cccagtttgt
    15721 tttataacgc caacaacttc ttttgctgct aatagttcgg gataaatcat tttatctatg
    15781 cctaaattta tgaaactgag tttgttaata ggattcaaat actccatatt attaactctg
    15841 gcaatggttt tttttgctcc aagttttttt gcgaaaatac aagataaaat attggtgtct
    15901 tgagagttag ttacagctat ataaaggtca gaagtgtgag tttgaacgct ttttagaact
    15961 gaaaagttgg tgcagtctcc acaaacagtc attaagtcat aagaggagac atcaacttct
    16021 tttattattg tgtcgttaat atcaattaaa gaaacatcgt gtttttcttt tacaagcatt
    16081 ttagctaaat aaagaccaat ttctccagct ccggaaatta ctatttgcat tttttatttt
    16141 ttaacttata aatattcatg aaaaatatct tccacttttt tagcatattt agtacaaaaa
    16201 ttatctgtat tagaaatttt taaacttttt attctaatat taaaatagtt taacagaaga
    16261 ttgtcataac ttgttgcttc aatatcatca atggttgtat ctattttaat ttgtgatttt
    16321 gatttaagcc tttcaagatg tctgttagca aggtctttag aaataattgc acctttttcg
    16381 ctagggaata ggaagaaaat aatgtcgttt gatttgtttt taaaaacctc tgaatgtatg
    16441 tctgcgttgc gataacatag tgcaaataat tctggtaaat ttattgggaa aacaggaatt
    16501 tgtatttcat cagcgagcat acaatataac acagctatat ttggtggtag aaatttttta
    16561 ttgctaatgc agtaggttat gcttaatgtg cttgatttga tttcttttac tgttagtttt
    16621 gtgtatccta atttgtcaaa aagagtagtg tttaaaacat ttattttttc aatatttgtt
    16681 agctggttac tcatttctat ccatatcgat gtctttagta tcgaatagtc tgtctgaagt
    16741 tctttggtgt ctatatctct gttaaagaat ttttcaatta aaaagactcc ttcataaaac
    16801 ttcgcttcag gatttgtaaa gtattctatt aattttgttt caaaactttc ccaaaaaatt
    16861 tcgtctaaaa tatcttctga cctttctaag gctaaactgt tagtggatag agcatgataa
    16921 ttttctaaat atgccttaaa aagttctcca tgtccaacaa tttcatcttt cactatagaa
    16981 aacacttttt cgtcagggtc ttctagtaat tttaataatg atatgatttt atttttatcc
    17041 atttaattat tcttctaatc ttgtaacttt ctcaacacca tcaattttta gcaaatcttt
    17101 cataatatct tctaagtctt gtaagttgtg aacatataaa attatttccc cactaaaaat
    17161 ttcgtcatca gtattgaaac ttaatgtttt tatatttact cttgagtcta aagaaattac
    17221 tttggtaatt ttattcaaca ttcctaattt atccatacct tcaatctgaa tttttgcttt
    17281 aaaagattga agtttgtagc tagtccattt taccgaaata attctatcgc cttgagttgc
    17341 agcaaggttt gttagtcttt cacaaattct tttatgaatg attatagact cgttaggatt
    17401 aacaaatcca gccacttcgt ctccgggtat tggattgcaa cattttgcta aagtaattcg
    17461 ctttgtgtca gcgttatcat caataataag agtgtttttt ttactttttg gttgacttgt
    17521 tctaaatttt ggaatgctga gtttccagta acttgcaaaa gttttaaagg tcttagctgc
    17581 ttgtttttcc aaatcatcta tttgaataag tcccgagcca attttaaaat agaaatcttc
    17641 tttatgtggc gtgttataag tttttcttag ttctttaaaa cgtttatggt ctaattttaa
    17701 gtttaattct cttacctttt taatgaaaat ttcttgtcct ttatctatat gatatcgtct
    17761 atcttctttt aaagaacttt tgattctatt ttttgctttt gttgtaattg caaaatttag
    17821 ccattctctt tttggagatt gtttgtcgga agttaggatt tctacttggt cgccactttt
    17881 taaaacatga gaaagaggtt ttaattggtg gttcactttt gcagcaatgg ctttgttgcc
    17941 aacttctgta tgaatttcgt atgcaaaatc aataacagta gcacctgatg gtattttttt
    18001 tatttcgcct ttaggagtaa aaacataaac ctctttagtg aaaatgttca ttttaacgtc
    18061 atctaaaaac gagagcgtat cattaacatt gttttctaaa gtttgctgta aagtgtttat
    18121 gagattatct aaatttccta gtgcgtcttt gttgtttgct ttgtatttcc agtgcgaagc
    18181 tagtcctttt tcggcgatat cgttcatttt tctacttcta atttgaactt caatccagtc
    18241 gccatttggt cccattactg taacatgtag tgcttcgtag ccatttgctt tgggttttgc
    18301 tatccagtct cgaactctat cttctaaatg tggataaata ttagttacta atgccattat
    18361 atgataacat ttcgcccttg ccttagaatc tggataatcg tcagggtgat caaatattat
    18421 tctgatggca aataaatcgt aaatttcttc aaaaggaaca ttcttagttc tcattttgtt
    18481 ccaaattgag tagatagatt ttgttctccc tataatatta gctttaatat tttcttcttc
    18541 caacaccttt tgaataggaa gttttatttg ctcaatatat ttatttcttt cttcttcggt
    18601 ttctgtaaat ttcttactta tttcccaata aatcagtggg tgttcgtatt ttaaagctaa
    18661 atcttctaat tcagttttta tagaatataa acctaatcga tgagctaatg gagcatataa
    18721 atatgttgtt tcgcttgcta tttttatttg tttatattct ggcatggagt ctaaagtacg
    18781 catgttgtga agtctgtctg ctagttttat gaatataact cttatgtctt gtgacattgt
    18841 taaaatcatt cttctaaaat tttcgacttg taaactcgca tctttgtcta aaatgtttgc
    18901 aatttttgtt aaccctccaa caattccagc aattgaagga ccaaaaagtg tttctatatc
    18961 atttagagta tagtctgtat cttcaacaac atcgtgtaat aaagcactaa caattgattt
    19021 tacgcctagt ccaatttctg aggctacaat cattgcaact gctattgggt gagtaaaata
    19081 aggttcgcca gattttcttc gcatttttga atgagcttcg tttgcaaggt caaatgcttt
    19141 gcgaactaaa tctatgtcat cttgagatct caagcgtttt gatactttta taagttcatc
    19201 aaactgcgac tcaatccata tttcttcttc ttttgttagt cccatacaaa aaagctaaaa
    19261 caaaatttat tttgtttaca aaaataataa aatatttcta aaatgttatt ttataaaaag
    19321 aaagaaaatg ccagttttaa tgtgttgttt atcattaaaa tcaaaatatt tcacataata
    19381 agtgtagtat tgagaagggg cagtatatgt cttgttgatt ttgccgtccc aagcttcttg
    19441 agagtcgttg gtctcaaaaa ctttcattcc aaatctatta taaacactaa actcataagg
    19501 ttcttctttt ataaaagata taatgggttt aaagactttg ttttcaggtt ctatagacga
    19561 aacattaaat gcgttaggta tccaaaaaac aggctcatga taaacacatg cgatatttga
    19621 gcgactacgt cctttactta ttattgctat ttctccttgt ttttcaacag cttcaacata
    19681 atagcagtat ttgctagatg tatttatttt tagattgtga cagtatttta cataccattc
    19741 tatattatat gtatagtcaa ataaattatt tggatttacg tcaatttcag cttcaatatt
    19801 atcaaaaatt ctatatattt tataattaaa tacaccattt gcccattgtt ggtactcatt
    19861 ccaacttaaa taatgattca aatcttctgc agtttctgct tttagtagaa tattacatgc
    19921 gtagtttgaa taggcattaa tatctccaca tggattgact gatgctattc tgtaataata
    19981 aatttcatta tttgtatcga tttcattgtc tgaaattaat attacatctt gactggtgtt
    20041 ttgtacaact tttattgttt tatagtctcc atcagggcta gttgctcttt gaattctaaa
    20101 ttctataatt tcagcagtat tatctacaac aaattttaag tctattttat tatcttttac
    20161 gcttgcttga ctagttatta aaaaatttgg agctctgtaa gcttctgttt ttactttaat
    20221 agaattagaa ctagctttat aattctcagg actaaccgct tcaatataat agtaatatgt
    20281 attgttggct tctaaagatt tatcttcata gctagtttta tctccagaaa ctttagttat
    20341 tcgttcgtaa gttccttcaa tatttcttct ataaatgtta tattccttta ctgtttgcca
    20401 gccttcatag tttgtccaat ttaaacttac gtatttgtta catttattat attcatgtgc
    20461 tagataaata gttgtatgtg gcgaggttat gttgctctta tagtattgat tgtcaaaaga
    20521 aactagtcta aatttttcgg attgttgata atttcctaga ccatatatat attcggtagt
    20581 ttctcttccc caaacagtat ctatagtctc tgttatagaa tttattattt gataaacaat
    20641 atatccttgc acatcaagcg aatcgctagg tagccatttt atgtgtacgc tagttgggtt
    20701 tgattctgga ataattgaag cggaaataaa tttgggagca tcgggaatat caatttgtcc
    20761 caatcctatg tttgagatag ctagagataa tatgatgaga aaaaatattc tttttatcat
    20821 tacttgtcaa aaatgttttt taaaaatcag tcaaaaataa taaattttac aaatactaaa
    20881 aaatttatgt gtttaaaatt aaaacatttt aattatttat aaatttttca cgaaaaatga
    20941 ttgattgtct aagtttactt gttcaaaacc catttttttt aaatattttg aatgtccttt
    21001 atgatatgat tttgttaaga gtttatctat tcctaatttt ttaaaataac ctgtattttg
    21061 tttgaaaata tattttccaa taacttcgtt tctatagtct ggtttagtgt aatcaacttc
    21121 aataagcatt tcatttttgt ttattttagc aattaaaatt cctgccatat cagcatttaa
    21181 catcattaaa aagcaacgtt gtcctttttc agcttgataa aatgggaaaa aactataaat
    21241 atctcttttg taattattga taaattcttg gatataagca ttgtcaaatt caacttctaa
    21301 tagttttatt gtagattttg ttttcgattt tttaataatg aaaattatat gtgagataat
    21361 tcctaaggct gctacaattc cgatagggta gaatggtcgc aaaaaagaat atacaacaat
    21421 agcaataagc gatatgaaaa ttaaatgttg cttaaatgct tttctattgc atataaatcc
    21481 tgctgttaga gttaggctgg ctataactat tattatatca atatattcca taaaaaaaca
    21541 ccgtaaaagt aagttttttt ttaattaaaa tcaagtgttt attacttgaa gttttaattt
    21601 tatactttta cggtgctttt agttatttag ttgggtttac atttttacaa ttttcattat
    21661 atagacattt tcattgtctt gtagtttgat aaaaatgtta cccttagcaa agttagacat
    21721 atctaaagtt tctattgaat cattaatttt gccagtttgt aaaacttttc cagttacatc
    21781 aattatttca taatttgtat tttggcaatt ttgaatattt aagatgtttt tagttgggtt
    21841 agggaaaata ttgattttac catgtccaaa atcttcaaca taactttcag caacttttac
    21901 tctaaatgat tgttctactt gatgaattcc atcagaagct tgaattttta cttgaaagcc
    21961 tattacactt ttttctgttg gagttccagt tattttacca gttccatcac cattatcaat
    22021 aatttcagcc caatctggaa gtgttgttgc agttagtgta attacatcac catctgcatc
    22081 agttgcttcg atattatatt cgtaaagttc gtattgaact ccacttgtaa ttggttcaga
    22141 aacaaactct ggaggtgtgt tggtcgtatg aacagttatt gtaaatactt gttctccaat
    22201 gtctgtgcca ttagatgctt gcaaagttac gttaaagttt tgacttattt gcgttatggt
    22261 aggagcagtt cctgttaaaa tagctgtgcc gtttccatta tcggttagtt ctaaccaatc
    22321 tggtttttct tgagcagtta ttgttaatgg gagtgtatta ggatcttctg ctgtaatttc
    22381 gtaagagtat tcttctcctt cattaatgct tgtaattggt aatgaagtga aatatggact
    22441 atcaagatta ggtattccta accatttgta aataccgtta gtgtttgcat ctaagttaaa
    22501 tccaccagcc catcctgtat tttggttaaa catttttacg tttgtatatt gaacagagtc
    22561 gtcaagcatt tcccaagtag ttccataatc taaactgtat gcagagaagt tatcttctat
    22621 ggtttgtgaa attttaacac caacatacat tcctggtttt ccaggaacgg cggatatgtc
    22681 gcttatgtat tggtcttcaa ctgggataat ttcccaagtt tcgccaccat cagtagttct
    22741 tattagtttc caactttgta tggttcctgt ggtttggtta taagtagcac aaatagctac
    22801 accattgttt tcatcagcaa atgaaatctt acttacatta gattgtccag ttgcgtgagc
    22861 agtccaagtt tgacctttgt cggttgagcg ataaacccta ccagtactag ttccaaacca
    22921 agcattatta ccaatagcac aattaactcc tgtccaaccc atttcatttg attgtggatc
    22981 aggaatatta ttgccgtcta taagtgtcca gttttcgcca ccatcatttg ttaaataaag
    23041 ttcaaattca ccatttacag ggtctccaat acaatagcca tcatttgcat taaacatatg
    23101 tactccatta caaaaactag aagagttgtt aaatgcagca cttgcttgat gtatccaagt
    23161 ttcaccaccg tctgaagttc tgtaaatgcc accacctcca ttagtgccgt cataaagagc
    23221 tacccaagcg ttcatatcat caattgcaga aatatctcca ggagaaagat tttcagggac
    23281 attgaatgta ttaggtatcc aagttgttcc
//
LOCUS       DAOPNX010000037        22228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_2921_length_22228_cov_6.609931, whole genome shotgun sequence.
ACCESSION   DAOPNX010000037 DAOPNX010000000
VERSION     DAOPNX010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22228
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2921_length_22228_cov_6.609931"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PKN53_05590"
     CDS             complement(<1..571)
                     /locus_tag="PKN53_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88456.1"
                     /translation="MKNIIIALVFVTISLLMACSVEKNTSSSRAYHNLVLKYNIYFNA
                     NEAYKRGINRINSSNTDNYNEILPVFVESKEDLASSAAGEMDKVIQKASKGIKIHSIT
                     KKPDMSGKNVSKKDKEFYDKKEFNKWVDDCYLIMGKAYFIKRDYLQARYNFEFIPRQF
                     PDLDTRHHANLYLVRTFSESKNYKEAKETL"
     gene            complement(636..>1130)
                     /locus_tag="PKN53_05595"
     CDS             complement(636..>1130)
                     /locus_tag="PKN53_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOD88457.1"
                     /translation="EKILEKADYVEKIADVIKDATNALYLGRGYLYPVALEGALKLKE
                     ISYIHAEGYPAAEMKHGPIALVDDKMPVIVVATKDHSYDKIVSNIQEVKARKGLIIAI
                     VTEGDTTIKNMADYVIEVPHSDFALAPLLTVIPLQLLSYYTAVKRGCNVDQPRNLAKS
                     VTVE"
     assembly_gap    1131..1230
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1231..2641)
                     /gene="glmS"
                     /locus_tag="PKN53_05600"
     CDS             complement(<1231..2641)
                     /gene="glmS"
                     /locus_tag="PKN53_05600"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOD88458.1"
                     /translation="MCGIVGYIGKRNASEVLIKGLKRLEYRGYDSSGIALASDDIRVF
                     KSKGKVSDLENLLNKENTKGSIGMGHTRWATHGEPTDDNAHPHESMNGHFVLIHNGII
                     ENYSKLKKGLGKKGYVFKSETDTEVLVNLIEYVFLNHANDDVPFTAEEAVRQALLMVE
                     GAYGLVIMAKSEKNKLIAARKGSPLVIGVGDGEYFIASDATPIVEYTNSVIYLNDGDI
                     AIITKNSLNLTNLKNVSLKPDIKKIDLKLDEIEKGGFEHYMLKEIHEQPRAIKDTYRG
                     RLNADAKVLTLGGLHSVFNELKSAERILIIACGTSWHAGLVGEYLLEEFTRIPTEVEY
                     ASEFRYRNPVIKKGDIVIAISQSGETADTLAAIQLAKSAGALVLGICNVVGSSIPRET
                     RAGVYTHAGPEIGVASTKAFTAQVTVLTMIAMAIGYANKGLTKKEYDDLIDELYVIPE
                     KIEKILEKADYVEKIADVIK"
     gene            complement(2654..3910)
                     /locus_tag="PKN53_05605"
     CDS             complement(2654..3910)
                     /locus_tag="PKN53_05605"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HOD88459.1"
                     /translation="MRIYNKYLCFFTLIVIGMFLFNSCEKKQSNIGLDLISDSDKLHL
                     YADTVDVKCYTVTDSVVSSDERTLSPLGSYFDPIFGFTKASFVCHARISSSNVDFSSV
                     SKINGIELHLKYSSTYGDTSTNQTINVCRLKKEVYFDTDYYSNFTLQPSEYDVLASET
                     LSFGKDSLTKIKLPNELAQEFVNSDINNFINNTTFIEYFNGLYVYTDPVSSNGCIYSI
                     NLIHPDSKMVMYYNDSLEYEFLINSKSAIINMYEHDYTNAHSDILSVLADSTLLLDKC
                     YIQSLAGLKAKIHFKQIIDYFGTIKNVAINKAQLVITLKADTDENIFLPPPKLALVAI
                     NENGKYDFLTDYKLNNSNFGGALENKFTYKFNIPFHIQELVNGKADNGIYLFASDNRT
                     IPYRLVLNANGASKDRMKLEVYYSLY"
     gene            complement(3910..4722)
                     /locus_tag="PKN53_05610"
     CDS             complement(3910..4722)
                     /locus_tag="PKN53_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOD88460.1"
                     /translation="MKLRVLFVTQAITPFMPETEMSLVGRHLPQGIQERDCEIRTFMP
                     NFGSINERRNQLHEVIRLSGMNLVIDDTDHQLVIKVASIQPARMQVYFIDNDDYFSRK
                     FALSEKGKEFEDNDERAIFYSRGVVETVKKLGWAPDIIHCHGWFTSLVPFYMRKIFDN
                     EPLFENGFKIIYSIYNDKFQKSLNEELPTKLIEDGVYPEDVALIETPSWENLMKFACE
                     YSDGVIVGTKLLDKNVANYLEGLEKPILNYQNPENYIDKYADFYDKLFKNKI"
     gene            4921..5766
                     /gene="panC"
                     /locus_tag="PKN53_05615"
     CDS             4921..5766
                     /gene="panC"
                     /locus_tag="PKN53_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOD88461.1"
                     /translation="MQIITSISQIIEARKQIAQNLKIGFVPTMGALHQGHISLVERSV
                     KENDITIVSIFVNPRQFNDKTDLENYPRNIENDIVLLEKAKCDIIFTPNNDDLYANYQ
                     GHKTDLKGIDKIYEGEFRPGHFQGVVDVVYRLFDLVKPHKAYFGEKDFQQLAIIKLMT
                     KNAKLPIEIIGCDIVREKSGLAMSSRNERLSEEERKNASEIYKTMLKNTANLKAGQKT
                     DEIAKKIKEEIDNIPFLKTEYIVFCEPETLKSINKFSANTDIRLCLAVWCKKIRLIDN
                     ISLHI"
     gene            5808..6158
                     /locus_tag="PKN53_05620"
     CDS             5808..6158
                     /locus_tag="PKN53_05620"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOD88462.1"
                     /translation="MNIEIVKSKIHKVRVTEANLQYVGSITIDEDLMKAANIIEHEKV
                     QVVNINNGERFETYVIKGEAGSGVICLNGPAARKVAVDDIVIIISYATMDFEEAKTFK
                     PSFIFPDTHTNRLI"
     gene            6161..6652
                     /gene="rfaE2"
                     /locus_tag="PKN53_05625"
     CDS             6161..6652
                     /gene="rfaE2"
                     /locus_tag="PKN53_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOD88463.1"
                     /translation="MNNLDLINSKIINPEKLQDFINSLKQNKETIVFTNGCFDILHYG
                     HINYLAKAANLGSKLIVGLNSDSSVQRLKGKDRPINKEFERAFVLASLFFIDAIIIFN
                     EDTPEKLIHNIAPDILAKGGDYKVEEIAGSDFVLKNGGKVEIIQFVEGYSSTKIINKV
                     SNI"
     gene            6691..7098
                     /locus_tag="PKN53_05630"
     CDS             6691..7098
                     /locus_tag="PKN53_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88464.1"
                     /translation="MKLLKITLLLVAGVLFFNSCKNYEEDIVGTWNFQTFDNTPQGTV
                     TWTFQDNGTLIRVSTASGDLKFDSCNYSIDKNLFKKHLIITNSKDMPGIASVNGKFRI
                     EVFKDDILKMTRFELEDGEKGGAYLRCELMRKQ"
     gene            7098..8450
                     /gene="radA"
                     /locus_tag="PKN53_05635"
     CDS             7098..8450
                     /gene="radA"
                     /locus_tag="PKN53_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOD88465.1"
                     /translation="MSKYKTVFVCQNCGYVTPKWAGRCNNCDSWNSFIEEVELKEDKQ
                     KNQIKAIPGKAYPILEIEKNTFERQNMFFTEFDRVLGGGLVPGSLVLLGGEPGIGKST
                     LILQSVLNLENRRVLYISGEESESQIKLRADRIGIKNTDCFIYTETNIQKILATIEEL
                     SPDLIIIDSIQTVASQDLSSSPGTISQIRECTRLLQEYAKSSSTPIILIGHINKDGDI
                     AGPMSLEHIVDVVLQFEGDSNYFYRLLRPKKNRFGSTYEIGVFEMTNTGLKEINDPGN
                     ILLSDENLNLSGVTFGTTAEGTRTLMIEIQALVGNAVFSSPQRNATGFDSRRFQMLLA
                     VIEKRLGLRLSQKDIFLNIAGGLKINDPAADLAVIAAVISSLYDKAPKENSCFIGEGG
                     LSGQIKPVSFIEKRVSEASRLGFNRIYMPNSQKSEVKIKNIELITISDITDFARKFLK
                     "
     gene            complement(8442..9158)
                     /locus_tag="PKN53_05640"
     CDS             complement(8442..9158)
                     /locus_tag="PKN53_05640"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOD88466.1"
                     /translation="MHLFYDKNIFSDFHVLNEEETRHCVKVLRLNIGDAVYLTDGMGN
                     FYETHIVENHAKKAILKIVKKTENYGKRNFNLHIAIAPTKMNERFEWFVEKSVEIGID
                     EITPVICGNSERKIIKTERINRVAEAAMKQSYKAYHPQINEQINLEKFLKQNLNYKQK
                     FIAHCSENDNKIYLGSVLEKQSSYLILIGPEGDFTENEIQMCKDAGFVEVSLGESRLR
                     TETAGVVTCSIVSVVNSILL"
     gene            complement(9259..10191)
                     /locus_tag="PKN53_05645"
     CDS             complement(9259..10191)
                     /locus_tag="PKN53_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88467.1"
                     /translation="MISLIKSIFAFIKSDFSWKIYLSIFLFTAALVLFEYNTFLYDKL
                     DMKFSYTPKVYLLNFLYFSFAYFVPVILVKSFSPSKIKLNTRFWIKGILGIIIISLYV
                     SFYSFYKLVYKLPSPDYYYWYYTLSNASGLLIVVLPLAIIYLIFDRKSEVPFYGANLK
                     HFNFRLALLLSVIAFLIALLGVKFSDVSNYYPIVNRTGYESFAAKHGISKIFSAGLFE
                     LSYMFDFAIVELIFRGFMIFAFVKFLGKKAVLPVAVLYTVIHFGKPLPETISSFFGAY
                     ILGIIAINQKNIGIGVVLHCVLAFSVEMLTLVKS"
     gene            complement(10194..11636)
                     /gene="dacB"
                     /locus_tag="PKN53_05650"
     CDS             complement(10194..11636)
                     /gene="dacB"
                     /locus_tag="PKN53_05650"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOD88468.1"
                     /translation="MKRLSVLFLVFSLCFNVFSQTKTAQDVLNDFISQSELKNASVSV
                     YAENLTTNTLVFDYNSNLALAPASVLKLVPTSMALELFGADYKFKTELAYSGQISADG
                     TLNGDLYIIGYGDPCLGSQNFTSTYGKDFEILQTWTNEVKNLNIKKIKGNIIADISYF
                     GEIPIPDDWIWEDIANYFGNQGSSLNFVDNLYYLHFKTGYTNGAATIITKIEPEGLGY
                     KFDNQVLSSSTVWDDAYIYYTADKNQMQVKGNLVWKKNDFVIKGSIREPERYLVQAFS
                     QNLNSNGISLDGNLKVISAFDINVPRKVFHTTYSPKLSDIVDYTNIRSFNLYAEMLAH
                     HIMKKTEKSWNESVVEFWKKKSIDTDGLELSDACGLSRFNAITTKQIVGMLKYMGLYS
                     ANKDKFFTSLPIAGTNGTMSRFCIGTKAQNRLHAKSGSIARVRSYAGYIYNSNGDEIV
                     FAVVVNNHSANSYRIKKIFEDFFAALVDIE"
     gene            11772..12749
                     /locus_tag="PKN53_05655"
     CDS             11772..12749
                     /locus_tag="PKN53_05655"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF005486.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HOD88469.1"
                     /translation="MQSKLFKNPNFSSINQLAKLRIPFVFVINFAKTDIRLFPIPANT
                     NDILYKFPNSTNCVKTKNSQNINLSIQETDFENYKQKFSYIQNKIKEGKITQINLTQA
                     SPITIDKTLKEIYFASNALYKLYLPNHFIVFSPETFVKIENNTLITHPMKGTIDASIK
                     NAEENILNDKKEIEEHQKVVAMVKSEFSNLSENVWVERSRYISKIKTKTKTLLQVSSE
                     ICAKLQPNWQDNLGDLLNSILPAASICGVPKNEAIEIINKIEGYDRGFYSGVMGIFTG
                     ESLDSGVMIRFIEKNGENYFYKSGGGITELSDVEKEFSELKNKIYVPIY"
     gene            12733..13320
                     /locus_tag="PKN53_05660"
     CDS             12733..13320
                     /locus_tag="PKN53_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOD88470.1"
                     /translation="MFQFIESIRLENGVVHNLNYHQERVNKTFLHIFKMPANFSLEKI
                     ISENKFPKQGLFKIRIVYDSHQQSIEFAEYTRPKFHTYKLVEIEKNFTYSFKSTDRKL
                     FDTISQETDKNTLPILIQNKNVSDSVFSNLIFEKNGNLYSPNTPLLFGTMLSQSLQQH
                     KINMITIKEDEVKNFDYIYPINAMNPLGAIEKIKI"
     gene            13370..14362
                     /locus_tag="PKN53_05665"
     CDS             13370..14362
                     /locus_tag="PKN53_05665"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOD88471.1"
                     /translation="MIYIVNKSNKPDYNIALEEYCFKNLLQHEKIFILWINEPAIIIG
                     KHQNAFEEINADYVKKHNINVVRRISGGGAVYHDLNNLNYTIISNEEKGVDFDFKTFS
                     QPVIDTLKELGVTAEFSGRNDILIDGKKICGNAQAYIKGRIMHHGCLLFNTELSVLAK
                     ALEIPKDIISSNATKSVRSAVDNILPNLPNKISVEEFANKLLAHMKRKFPAMKEYTFS
                     QQELAEIEKNRASKFGTWEWNWGKSPKFDINRHSRYTAGKIQVFANVKNSMIENIKFY
                     GTFFGNNSNLEEIENALIGVRYTYTNIENKLKDLNFNDYFAGFSLEELTKAIVD"
     gene            14374..15420
                     /gene="mtnA"
                     /locus_tag="PKN53_05670"
     CDS             14374..15420
                     /gene="mtnA"
                     /locus_tag="PKN53_05670"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOD88472.1"
                     /translation="MKVNNKHYRAIWVENETVFMIDQNKLPFEFKIFKSKNLQTTCMA
                     IKTMITRGAGSIGAAAAYAMLQAIYENKSQDLYSSLEKAKKLIDQTRPTAVNLFYATQ
                     RVLDAAKISKEFAISEAHAIADECAEHGRLIGEFGNTLIEDGFSIETHCNAGWLALVD
                     YGSALAPIYAAHNSGKNIFVYVDETRPRNQGARLTAWELKQENIPHAIIPDNAGAYYM
                     SKGKIDLFIAGADRIAANGDTANKIGTLEKAIAAKYYGIPFYIAAPSSTIDLKTKTGK
                     SIPIEERSADEVIYCSGKLKSGKIEEMQITNPGSGALNPAFDTTPAELITGIITEKGI
                     IKPNEEEIIKLFQK"
     gene            15417..16040
                     /locus_tag="PKN53_05675"
     CDS             15417..16040
                     /locus_tag="PKN53_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOD88473.1"
                     /translation="MMFKKERKEVAKIMRRLYAQGLTTSSGGNVSFKNENGFVFITAS
                     QTDKSCIKWKDIIVFDDNKNNITPKLKPSMESGMHLKIYETRKDISAIVHSHPVYASS
                     YAVAGSIPNTALTGEASLLLGKISLSEYELMGTEKLAVEVANKLQDTDVCIMKNHGAI
                     SIGKSLFEAFDKMEVLEFVCHLNINSLILNKVNKLSPEQIQEINKSF"
     gene            16247..16705
                     /locus_tag="PKN53_05680"
     CDS             16247..16705
                     /locus_tag="PKN53_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88474.1"
                     /translation="MKTKKFIKIDLYHICDLLAIITLIFLAVFLVNKTKLDYFLFFAG
                     IACLTTSLQLHFGQAAIKNKSNKTLKAKPEDEDKDFLINVEPHSKVFQIDGIKVANKV
                     YKIPDSIHVKVTKKNKVRVSSFCAYMIYWLYNGGILDVAPDEKWNKLFEA"
     gene            complement(16761..17159)
                     /locus_tag="PKN53_05685"
     CDS             complement(16761..17159)
                     /locus_tag="PKN53_05685"
                     /inference="COORDINATES: protein motif:HMM:NF036886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5618 family protein"
                     /protein_id="HOD88475.1"
                     /translation="MKKKDSIEKARQYVEKAKKILEKNGKFYEATETYQNNKCVKRAG
                     NRLWKACLIALEAAFPNVEGKGRILVDKYKEVAQCDKKLHDCIFNTYYITYLAMGYDG
                     TSVKTITDVGFEYANLLIDHCAILCNQQKI"
     gene            complement(17347..17418)
                     /locus_tag="PKN53_05690"
     tRNA            complement(17347..17418)
                     /locus_tag="PKN53_05690"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17383..17385),aa:Arg,seq:cct)
     gene            complement(17457..19496)
                     /locus_tag="PKN53_05695"
     CDS             complement(17457..19496)
                     /locus_tag="PKN53_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HOD88476.1"
                     /translation="MSFINPAFFWAMFAIAVPIIIHLINFRRHKTLYFSNTRFLENIR
                     KETKTKTKLKQILILIARILTIAMLVIAFAGPFIPIKQGNNDKPSGLNIIYLDNSFSM
                     ENTGKKGKLFEQAQQIAQEIAYSSASSAKYFLITNDLLPEHQYFSDRDAFVRNINKTS
                     ISPSSYTLNEVVLKANANVTKEQNANLYVISDMQETFVENISAKLNDNINLIFVPLKA
                     EKQNNIFIDSVWFESLSHRLNMSETLVARLVNNSDEAFFEQSLQLYINDSLKAVAGFN
                     IEAGETKDVEIEYVNTQSGFIEARLEITDYPIIYDNILYFNYKVSDMTKILIINNEKS
                     NRWLTALYASDSENFDLKQVKLGTEQNENFNNYNLVILNSFSEISTGLSANLLEYTEN
                     GGSLIFIPDRKLKVSSINSFLNLFNAGNFTEQSIQNTRIASIEYEHELFKNVFIKKEK
                     QSELPIVANSHKFNTFTSSSVRKVLNLENNATLLAAGNYGSGKIYIISAHVDESNSEF
                     VKSAIFAPAFYNMALNSQISNQLYSTIKAGAKVEIKSDKEILSSDIFKLTDKTNFEIL
                     TPHRYANKKIIIDIPQQILNSGTYSLYKTEEYISPISLNYDRSESVLDFVSASDLDKL
                     IVEKFDNKAKLVDSDKSDFKLELKRFADGKPLWQLFVLLAFIFVCCEIAFIRLLK"
     gene            19639..20451
                     /locus_tag="PKN53_05700"
     CDS             19639..20451
                     /locus_tag="PKN53_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HOD88477.1"
                     /translation="MAKINVKNTEITIISIEEKDYISLTDMANAKESESRAADIIKNW
                     LRNRYTLEFLGTWEQIHNPIFKVVEFDHFKMQAGLPNFVLSVSEWIEKTNAIGIIVKK
                     GKYGGTFAHKDIAFEFGSAISVPFKLYLINEFQRLKEEEQKQLGWSAKRELAKLNYHI
                     HTDAIKQNLIPQELSTTQTSIIYANEADVLNVALFGITAKQWRDSNPELKGNIRDYAT
                     INELICLSNMENLNAVFINEKMPQKERLIKLNQIAIQQMSILQEVENRKLLK"
     gene            20576..20896
                     /locus_tag="PKN53_05705"
     CDS             20576..20896
                     /locus_tag="PKN53_05705"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOD88478.1"
                     /translation="MNTRDVIISKILELSKEKYPDAEIYLYGSHARGDAKPFSDWDLL
                     ILLNLKQIPFALETKIMDEFYEIELETGEILSPLIYPKEEWNVKYRQTPLYENIKNES
                     IRLI"
     gene            21142..21939
                     /locus_tag="PKN53_05710"
     CDS             21142..21939
                     /locus_tag="PKN53_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88479.1"
                     /translation="MKKILITLVLSILFLNSIAQELELKKKWSLGFSYTPNISYLYNP
                     TVYFGGQVPTGYSYQSKKSVRDLPIFEFMNFGIRISAVLNEKNEIETGVILSNKDYYS
                     PQGLIHDEMPFARFKPRAYFIDVPLIYKHNISKIGNYNLNLIGGVIGSVPLYKHIDFP
                     GELYLYATDGNEFALTQNLDFYPLCMNFNLGLRLLKNKINNIQFEFGPVFQSAIMPFS
                     RKIEGNIIKVRGNHELIVSNPEKGYMPCFIGFDLILRFNFFDLTKYK"
BASE COUNT         8153 a         3433 c         3107 g         7435 t
ORIGIN      
        1 ctaatgtttc cttagcttct ttataatttt tactttctga gaatgtacga actaaataaa
       61 gattagcatg gtgtcgagta tctaaatcag gaaattggcg aggaataaat tcaaaattat
      121 acctagcttg caaataatct cttttaataa agtaggcttt acccataatc aagtaacaat
      181 cgtccaccca tttattaaat tcttttttgt cataaaattc tttatctttt ttcgacacat
      241 ttttgccact catatctggc ttttttgtta tcgaatgaat ttttatacct ttagatgctt
      301 tctggataac tttatccatt tcgccagctg cagaacttgc caaatcttct ttactctcta
      361 caaaaacagg caaaatttca ttataattat ctgtattgct ggagtttatc ctatttatcc
      421 ctcttttata tgcttcgtta gcattaaaat aaatattata tttcaacaca agattatgat
      481 aagctctact tgaagaagta tttttttcga ctgaacaagc catcaaaaga cttattgtta
      541 caaaaactaa ggctattatt atatttttca atttcataaa tcttacaaga tgataactta
      601 aaatatttat ttttattatt tataaagcat aaaatttatt caacagttac tgattttgcc
      661 aaattcctag gctggtcaac attacaaccg cgttttaccg cagtataata cgataataat
      721 tgcaatggaa taacagttaa caatggtgct aatgcaaagt cggaatgagg aacttctata
      781 acataatcag ccatgttttt aattgttgta tctccttcgg ttacaatagc tattatcaag
      841 ccttttcttg cttttacttc ttgtatatta gatacaattt tatcgtaaga atggtctttt
      901 gttgcaacca caatcacagg cattttatca tctacaagag ctattggtcc gtgtttcatt
      961 tcagcagctg gatatccttc tgcatgtata tatgatattt cttttaactt taaagctcct
     1021 tccaatgcta ctggatataa atatcctcta cctaaataca aagcattggt tgcatcttta
     1081 attacatctg caattttttc gacataatct gctttttcta atattttttc nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctttaattac atctgcaatt ttttcgacat
     1261 aatctgcttt ttctaatatt ttttcgattt tttctgggat tacatacaat tcatcgatta
     1321 aatcgtcata ttcttttttg gttaatcctt tatttgcata accaatagcc atagctatca
     1381 ttgttaaaac tgttacttga gctgtaaaag cttttgttga agctactcct atttctggtc
     1441 cagcatgtgt ataaactccg gcacgtgttt cgcgtggaat acttgaaccc accacattac
     1501 atatacccaa gaccaaagca cctgcagatt ttgctaattg aattgcagca agtgtgtcgg
     1561 cagtttcacc actttgcgag atagcaataa ctatatctcc tttttttatt acaggatttc
     1621 tatatctaaa ttccgaagca tattcaactt ctgtaggaat gcgtgtgaat tcttctaata
     1681 aatactcccc tactaaacca gcgtgccaac tagtaccaca agcaattatt aaaattcttt
     1741 cagcggattt aagttcatta aaaactgaat gtaatccacc aagtgttaaa accttagcat
     1801 cagcattcaa cctacctcta taagtatctt taattgcacg tggttgctca tgaatctctt
     1861 taagcatata atgctcaaaa ccgccttttt caatctcatc aagttttaaa tctatttttt
     1921 taatatctgg ttttagagaa acatttttta agtttgttag atttaaacta ttttttgtaa
     1981 taatagctat atcgccatca ttaagatata ttacgctatt tgtatattca actattggcg
     2041 tagcatcaga ggcaataaaa tattctccgt ctcctacacc tataactaaa ggacttcctt
     2101 tgcgagctgc aataagttta tttttttctg acttagccat aattactaaa ccataagcac
     2161 cttcgaccat caataaggct tggcggacag cttcttcggc agtaaacgga acatcatcat
     2221 tagcatggtt taaaaaaaca tattctatca aattaactaa aacttcagtg tcggtttcag
     2281 acttaaaaac atatcctttt tttccgagac ccttttttag ttttgagtaa ttttcaataa
     2341 tgccattatg aattagtaca aaatgtccat tcatagactc atgaggatgt gcattatcat
     2401 ctgtaggttc gccatgtgta gcccaccttg tatgccccat accaatagaa cctttagtat
     2461 tttctttatt taaaagattt tctaaatcac ttacttttcc tttgctttta aaaactctaa
     2521 tatcgtcaga agccaatgca attccagaag aatcatatcc acgatattct aaacgcttta
     2581 gtccttttat tagtacttca cttgcgtttc tttttcctat atatcctaca ataccacaca
     2641 taattactaa attttaatac aatgaataat aaacttccaa tttcattcta tccttagatg
     2701 ctccatttgc atttagaaca agtctatatg gtattgttct attatcgctg gcaaataaat
     2761 aaattccatt atctgctttt ccattaacaa gctcttgaat atgaaaaggg atattaaatt
     2821 tataagtaaa cttattttct aaagctcctc caaaatttga attatttaat ttatagtctg
     2881 tcaaaaaatc atatttacca ttttcgttaa ttgccactaa agctaatttt ggaggtggta
     2941 aaaatatgtt ttcatcagta tctgctttga gagtaattac taattgagct ttatttattg
     3001 caacgttttt aatagtgccg aaataatcaa taatttgttt aaaatgaatt tttgctttta
     3061 aacctgccaa actttgtata taacatttat ctaacaataa agtagaatct gccaacacac
     3121 ttagtatgtc cgaatgagca tttgtataat catgttcata catatttatt attgcagact
     3181 ttgaattaat taaaaattca tattctaaag aatcattata atacatcacc attttagaat
     3241 cgggatgaat taaatttata ctataaatac aaccattgct actaactggg tctgtataaa
     3301 catataaacc attaaagtat tcaataaatg tagtattatt tataaaatta tttatatccg
     3361 aatttacaaa ctcttgtgct aattcatttg gaagcttaat ttttgttaaa ctatcttttc
     3421 caaaacttaa agtttcgcta gctaaaacat cgtattccga tggttgtaaa gtaaaattgg
     3481 aataataatc tgtatcgaaa tagacttctt ttttcaatct acaaacatta atggtttggt
     3541 tagttgaagt atctccataa gttgaactat attttaaatg taattctata ccatttattt
     3601 tacttactga agagaagtca acattactcg aagatattct tgcatgacac acaaaagaag
     3661 cttttgtaaa accgaatatc gggtcaaaat aagaacccaa aggtgacaaa gttctttcat
     3721 ctgacgacac tacagagtct gtaactgtat aacactttac gtccactgtg tcagcataaa
     3781 gatgtagttt atcgctatct gatattaaat ctaagccaat attactttgt tttttctcgc
     3841 aagaattaaa taaaaacatg cctattacta ttaaagtaaa aaagcacaaa tacttattat
     3901 aaattcgcat taaattttgt ttttaaacaa tttatcgtaa aagtcagcat atttatcaat
     3961 atagttttcg ggattttgat agttaagtat aggtttttct aatccctcta gatagtttgc
     4021 aacgtttttg tctaataatt ttgtaccaac aattacacca tcagaatatt cgcaagcaaa
     4081 tttcattaaa ttttcccagg aaggtgtttc tattaaagct acatcttctg gataaactcc
     4141 atcttctatt agttttgttg gcaactcttc gtttagtgat ttttgaaact tatcgttata
     4201 gattgaataa ataattttaa agccattctc aaatagaggt tcattatcaa aaatctttct
     4261 catataaaat ggcactaaag aagtaaacca accgtggcaa tgaataatat caggagccca
     4321 gcctagtttt ttaacagttt cgaccacacc acgagaataa aaaattgctc tttcatcgtt
     4381 gtcttcaaat tcttttcctt tttctgataa agcaaatttt ctgctaaagt aatcgtcatt
     4441 atctataaaa taaacttgca tacgcgccgg ctgaatagaa gccactttaa ttactaattg
     4501 atggtcagta tcatcaatta ccaaattcat tccactcaac ctaattactt catgtaattg
     4561 atttcttctt tcatttatgc ttccaaaatt tggcataaac gttctaattt cacaatctcg
     4621 ctcttgaatt ccctgaggca aatgacgccc gaccaaagac atctctgttt ctggcataaa
     4681 aggcgttatt gcttgagtaa caaaaagaac tcttaatttc atatattttc ctttattttt
     4741 ataaaccaca aaaatatata aaaaatttca attttccata cttttttatt aagatattat
     4801 taaacagata attatcaatt aattaggtaa aaataaacta tcacaagaaa acagttttac
     4861 aacaagtttt taatactttg tttttctttt taataatctt aaatttgcaa aaaaaataaa
     4921 atgcaaatta ttacaagtat ttctcaaatt attgaagctc gtaaacaaat tgcacaaaac
     4981 ttaaaaatag gttttgttcc aactatggga gctttacacc aaggacatat ttctttggtt
     5041 gaacgttctg ttaaagaaaa cgacattaca atagtatcta tttttgtaaa ccctcgacaa
     5101 tttaacgaca aaactgattt agaaaattat ccaagaaata ttgaaaacga tattgtttta
     5161 ttagaaaaag caaaatgcga tataattttc acaccaaaca atgatgattt atatgcaaat
     5221 taccaagggc ataaaacaga tttaaaaggc attgacaaaa tttatgaagg tgaatttaga
     5281 cctggacatt ttcaaggagt tgttgatgtt gtttacagac ttttcgactt agttaagcct
     5341 cacaaagcat attttggtga aaaagatttt caacaattag caataataaa gctaatgaca
     5401 aaaaatgcta aattgccaat agaaattata ggttgcgata ttgttagaga aaaaagtgga
     5461 ttagcaatga gttcaagaaa cgaaaggctt agtgaagaag aaagaaaaaa tgcttcagaa
     5521 atttataaaa caatgctaaa aaatacagca aacttaaagg ctggacaaaa aactgacgaa
     5581 attgccaaaa aaattaaaga agaaatcgac aatattccat ttttaaaaac agaatatatt
     5641 gttttttgcg aaccagaaac tttaaaatca attaacaaat tctctgcaaa cactgatatt
     5701 agactatgtt tagcagtttg gtgtaaaaaa attagattaa ttgacaacat aagtttgcat
     5761 atttaaacaa gtatttttta actttgcaac gaatttattt tagtatcatg aatatagaaa
     5821 tagtaaagtc aaaaattcat aaagttagag ttacagaagc taacttacaa tatgttggta
     5881 gtattaccat tgatgaagat ttgatgaaag ctgcaaatat tattgaacac gaaaaagttc
     5941 aggtagttaa cataaataat ggcgaacgct tcgaaactta tgtaattaaa ggcgaagctg
     6001 gctcaggagt tatttgctta aatggaccag cagcaagaaa agttgctgtt gacgacatag
     6061 ttattataat atcttatgct acaatggatt ttgaagaagc aaaaactttt aaaccttctt
     6121 ttattttccc cgacactcat acaaatcgtt tgatataact atgaataatt tggatttaat
     6181 aaattcaaaa ataataaatc ctgaaaaatt acaagatttt atcaattctt taaaacaaaa
     6241 taaagagacc attgtcttta caaacggttg ttttgacata cttcattatg gacatataaa
     6301 ctatttagca aaagctgcaa acttaggctc taaacttatt gttggattaa attctgattc
     6361 ttcagttcaa agattaaaag gcaaagatag acctataaac aaagaatttg aaagagcttt
     6421 tgttttagcc tcactttttt ttattgatgc tataataatt tttaatgaag acactccaga
     6481 aaaattaatt cacaatatag ctccagacat attagcaaaa ggcggagatt acaaagtaga
     6541 agaaattgca ggttccgatt ttgtactaaa aaatggagga aaagtagaaa taattcaatt
     6601 tgttgaagga tattcttcaa caaaaattat aaataaagta tctaatattt aaaatttaat
     6661 tatatttgta cgcttaaaaa atactaagtt atgaaactat taaaaattac tttattgtta
     6721 gttgcaggag ttttattctt caatagctgc aaaaattacg aagaagacat tgtcggcact
     6781 tggaatttcc aaacatttga caacacacca caaggtacag taacttggac ttttcaagat
     6841 aatggcactt taataagagt ttcaacagca agtggagacc taaaatttga ttcttgtaat
     6901 tatagtattg ataaaaactt atttaaaaag cacttaataa ttaccaatag taaagacatg
     6961 ccaggaattg caagtgtgaa tggcaaattc agaatagaag tttttaaaga tgatatttta
     7021 aaaatgacta ggtttgaact tgaagacgga gaaaaaggtg gtgcttattt gcgttgtgaa
     7081 ctcatgcgta aacaataatg tcaaaatata aaacagtttt tgtttgtcaa aactgtggat
     7141 atgttacccc taaatgggct ggcagatgca acaactgcga cagttggaat tcttttatcg
     7201 aagaagttga attaaaagaa gataaacaaa aaaatcagat taaagctata ccgggcaaag
     7261 cttatcctat tcttgaaatt gaaaaaaata cttttgaaag acaaaatatg tttttcaccg
     7321 aatttgacag agttcttggt ggaggtttag tgccaggctc tttagtttta cttggaggcg
     7381 aacccggaat tggaaaatca actttaattt tacaatcagt tttaaattta gaaaatcgta
     7441 gagtacttta tatttctggc gaagaaagcg aaagccaaat aaaactaaga gccgacagaa
     7501 ttggaattaa aaatactgat tgctttattt atacagaaac aaatatccaa aaaatattag
     7561 caacaataga agaattaagc ccagatttaa taattataga ttctatacaa accgttgctt
     7621 ctcaagacct tagctcatcg cctggaacta tttcgcaaat tagagaatgt acacgtcttt
     7681 tacaagaata cgcaaaatca agttctacac caataatttt aatcggacat ataaataaag
     7741 acggcgatat tgctggacca atgtccttag agcatattgt tgatgtagtt ttacaatttg
     7801 aaggagacag caattatttt tacagactat tacgccctaa gaaaaaccgt tttggctcaa
     7861 cttacgaaat tggtgttttt gaaatgacaa acactggact taaagaaatt aacgaccctg
     7921 gaaatatcct tttatctgac gaaaatttga atttaagcgg agttactttt ggcacaactg
     7981 ctgaaggcac acgtactctt atgattgaaa ttcaagcttt agttggaaat gcagttttta
     8041 gcagtccaca acgcaatgct acaggatttg actctagacg ttttcaaatg ttgcttgcag
     8101 ttattgaaaa aagattaggt ttaagacttt cgcaaaaaga tatattttta aatattgctg
     8161 gcggattaaa aataaacgac ccagcagcag atttagcagt tatagcagcg gtaatttcat
     8221 cgctttatga caaagctcca aaagaaaact cttgctttat aggcgaagga ggcttatcag
     8281 gacaaataaa accagtatca tttattgaaa aacgagttag cgaagcctca agattaggtt
     8341 ttaatagaat ttatatgcct aactcacaaa aatctgaagt aaaaattaaa aacattgaac
     8401 taattactat ttcagatatt actgatttcg caagaaaatt tttaaagtaa aatagaattt
     8461 actaccgaca caatactaca agtaacaaca ccagcagttt cggttcgcaa acgactttcg
     8521 ccaaggctaa cttcaacaaa tccagcatct ttacacattt gaatttcatt ttctgtaaaa
     8581 tcgccttcgg gaccaattag aattaaataa gaagattgtt tttccaaaac tgagccgaga
     8641 taaattttat tgtcattttc agaacaatgt gcaataaatt tttgcttata gttcaagttt
     8701 tgttttaaaa atttttccaa atttatttgc tcgtttatct gtggatgata agccttgtaa
     8761 gattgcttca ttgcagcttc ggcaactcgg tttattcttt cagtttttat aatttttctc
     8821 tcagaatttc cgcaaattac tggagtgatt tcatcaattc ctatttccac agatttttca
     8881 acaaaccact caaatctttc attcatctta gttggagcaa tagcaatatg caaattaaaa
     8941 tttcgttttc cgtagttttc tgttttttta acaattttta aaattgcttt tttagcatga
     9001 ttttctacaa tatgagtttc ataaaaattt cccatgccat ctgtcaaata aactgcatcg
     9061 ccaatattta atcttaaaac cttaacgcaa tgccttgtct cctcctcgtt aaggacatga
     9121 aaatcactaa aaatattttt atcgtaaaat aagtgcataa aatctttttg caaaaataaa
     9181 caaaattcag ttacaaaatt cagttaaaag tttataaagt aaaaagttca taaagaaagc
     9241 cgaaccctca agtctctttt aacttttaac taatgttagc atctctactg aaaatgctaa
     9301 cacacaatgc aacaccactc ctatcccaat atttttttga ttaattgcaa taatacctaa
     9361 aatataagca ccaaaaaacg agctaatagt ttctggcaaa ggttttccaa agtgaattac
     9421 tgtatataaa actgctacag gtaaaacagc ttttttgcct aaaaacttta caaaagcgaa
     9481 tatcataaat cctcgaaaaa tcaactcaac aattgcaaaa tcgaacatat aagacaattc
     9541 aaataaacct gcagaaaata ttttgctaat tccatgtttt gctgcaaagc tttcgtaacc
     9601 agttctattt acaataggat agtaatttga aacatcagaa aatttaactc caagtaaagc
     9661 aatcaaaaaa gcaattacag acaataataa agctaaacga aaattaaaat gtttcaaatt
     9721 agctccataa aatggcactt cgctttttct atcaaaaatt aaatatatta ttgccaaagg
     9781 taaaactact atcaacaacc ccgaagcatt acttaatgtg taataccaat aataataatc
     9841 tggactcgga agtttataaa caagtttata aaaagaataa aaagaaacat aaaggctaat
     9901 aataataatt cctaaaatgc cttttatcca aaaccgagtg tttaatttta tttttgaagg
     9961 actaaatgac tttaccaaaa ttactggcac aaaataagca aacgaaaagt ataaaaaatt
    10021 caataaatat acttttggtg tataagaaaa cttcatgtcc aatttatcat acaaaaaagt
    10081 attgtattca aacaaaacta aagcagcggt aaaaaggaaa attgacaaat aaattttcca
    10141 agaaaaatct gactttataa atgcaaaaat tgattttatt aaacttatca tatctactct
    10201 atgtcaacta atgcagcaaa aaaatcttca aaaatctttt taattctata cgaatttgca
    10261 gaatgattat ttacaactac agcaaaaaca atttcatcac catttgaatt atagatataa
    10321 cctgcataag acctcactct tgcaatactt cccgattttg catgaagtcg attttgagct
    10381 tttgtaccaa tacaaaatct tgacatagtt ccattcgtac ctgctattgg caagcttgtg
    10441 aaaaacttat ctttatttgc agaatataat cccatatatt tcaacattcc tactatttgc
    10501 ttagttgtta ttgcattaaa acgacttaaa ccacaagcat cgctaagttc taaaccatca
    10561 gtatcaatag atttcttttt ccaaaattct acaacagact cattccaaga tttttctgtt
    10621 tttttcataa tgtgatgagc taacatttct gcatataaat taaaacttct aatgttagta
    10681 taatcaacaa tatcagaaag ttttggcgaa taagttgtat gaaaaacttt tcgtggaacg
    10741 ttaatatcaa atgcacttat tacttttaaa ttgccgtcta aacttattcc attagaattc
    10801 aaattttgag aaaaagcttg aacaagataa cgttcaggct cgcgaataga acctttgatt
    10861 acaaaatcat tttttttcca aactaaattt ccttttactt gcatttggtt tttatccgct
    10921 gtataataga tataagcatc gtcccaaaca gttgaagaag aaagcacctg attatcaaac
    10981 ttataaccta agccttcggg ttctattttt gtaataattg tagctgcacc atttgtatat
    11041 cctgttttaa aatgtaagta atataaatta tccacaaaat ttaaagacga gccttgattt
    11101 ccaaaatagt ttgcaatatc ttcccaaatc cagtcgtcag gaattggaat ttcgccaaaa
    11161 tatgagatgt cagcaataat atttccttta atttttttaa tatttagatt tttaacttca
    11221 tttgtccatg tttgtaaaat ctcaaaatct tttccataag ttgaagtaaa attctgagaa
    11281 ccaagacaag ggtcgccgta tccaataatg tacaaatctc catttaaagt tccgtcagca
    11341 gatatttgtc ctgaataagc aagctctgtt ttaaatttat aatctgcacc aaacaattct
    11401 aaagccatgc ttgtaggaac aagttttaaa accgaagcag gtgctaaagc caaatttgaa
    11461 ttataatcaa aaactaaagt attagttgtt aaattttctg cataaacaga tacagaagca
    11521 tttttaagtt cgctttgtga aataaaatcg tttaaaacat cttgagcagt ttttgtttgt
    11581 gaaaacacat taaaacataa actaaaaacc aaaaataaca cagacagtcg tttcatatta
    11641 aaaaaattat ttcagtgcca aatatataaa aacatagtgt aaaatataag tattgcaaaa
    11701 aattttatca aaaaatttat ttacataaaa acaaataata tttttgcttc aatttacaaa
    11761 tgaaatataa aatgcaatct aaacttttta aaaatccaaa ttttagttca ataaaccaat
    11821 tagctaaatt acgtattcct tttgtttttg taataaattt tgcaaaaacc gacattcgct
    11881 tatttcctat tcctgcaaat acaaacgata ttttgtacaa atttccaaac agcactaatt
    11941 gtgtcaaaac taaaaattca caaaatatta atttaagtat tcaagaaact gattttgaaa
    12001 actacaagca aaaattctca tatattcaaa acaaaataaa agaaggaaaa atcactcaaa
    12061 taaatctaac acaagcaagt ccgattacaa ttgacaaaac tcttaaagaa atttattttg
    12121 cttcaaacgc tttatataaa ctatatcttc ccaatcattt tattgttttt tcgcctgaaa
    12181 cttttgtaaa aattgaaaac aacacgctca tcacccaccc tatgaaaggc actatagatg
    12241 cttcgattaa aaatgccgaa gaaaatattt taaacgacaa aaaagaaatt gaagaacacc
    12301 aaaaagttgt tgcaatggta aaatcagaat tttctaattt gtccgaaaat gtttgggtag
    12361 aaagaagtcg ctatattagc aaaattaaaa ctaagacaaa aactttatta caagtaagtt
    12421 ctgaaatttg tgcaaagcta caaccgaatt ggcaagataa tttaggcgat ttattaaact
    12481 ctatacttcc tgctgcatca atttgcggtg ttcctaaaaa cgaagctatt gaaataatta
    12541 ataaaattga aggctacgac agaggttttt atagcggtgt tatgggaatt ttcactggcg
    12601 aaagtttaga ttctggagta atgattagat ttattgaaaa aaatggagaa aattattttt
    12661 ataaaagtgg cggtggaatt actgaactaa gcgatgtaga aaaagaattt agcgaattaa
    12721 aaaacaaaat ttatgttcca atttattgaa agtatacgcc tcgaaaatgg agttgtacat
    12781 aacttaaact accatcagga aagagtaaat aaaacttttt tacatatttt taaaatgcct
    12841 gccaacttct ctctcgaaaa aattatttct gaaaataaat ttcccaaaca aggacttttc
    12901 aaaataagaa ttgtctatga ttcacaccaa caatcaatag aatttgcaga atatacaaga
    12961 ccaaaatttc acacttacaa attagttgag atagaaaaaa atttcacata ttcatttaaa
    13021 agtactgata gaaagctttt cgacacaatt tctcaagaaa ctgataaaaa tactttacca
    13081 attttaattc aaaacaaaaa tgttagcgat tctgtattca gcaatcttat atttgaaaaa
    13141 aatggaaatt tatattcgcc aaacactcca ctactttttg gcacaatgct aagtcaaagc
    13201 ttgcaacagc acaaaatcaa catgattaca ataaaagaag atgaagttaa aaattttgat
    13261 tatatttatc ctatcaacgc tatgaatcca cttggagcaa ttgaaaagat taaaatatag
    13321 tctgattatc tccaaaaatt cttaattttg cctgtctaaa atttttatta tgatttatat
    13381 tgtaaacaaa tccaacaaac ctgattacaa cattgcttta gaagaatatt gttttaaaaa
    13441 tcttcttcag cacgagaaaa tatttatttt atggataaac gagcctgcaa ttattattgg
    13501 caaacatcaa aatgcatttg aagaaattaa tgcagattat gtaaaaaagc ataatattaa
    13561 tgttgtaagg cgaatttctg gtggtggagc tgtttatcac gatttaaaca atttgaatta
    13621 cacaattatt tctaacgaag aaaaaggagt tgattttgat tttaaaacat tttctcaacc
    13681 tgtaatcgac actctaaaag aattaggtgt tacagcagaa ttttcgggta gaaatgatat
    13741 tttaattgat ggaaaaaaaa tctgtggaaa cgctcaagcc tatattaaag gtagaattat
    13801 gcaccacggc tgtttgttgt tcaacactga actaagtgtt ttggcaaaag ctttagaaat
    13861 tcctaaagat attattagct ctaatgctac aaaatctgtt cgtagcgctg ttgataatat
    13921 tttgccaaat ttgccaaaca aaattagcgt tgaagaattt gctaacaaac ttcttgctca
    13981 catgaaacgc aagtttccag caatgaaaga atatactttt tcacaacaag aattagctga
    14041 aattgaaaaa aacagagcaa gcaaatttgg aacttgggaa tggaattggg gaaaatcgcc
    14101 aaagtttgat ataaacagac attcgagata cacagctggg aaaatacaag tatttgctaa
    14161 tgtgaaaaat tccatgatag aaaatattaa gttttacggt actttttttg gcaacaatag
    14221 caatctcgaa gaaattgaaa atgcactaat tggcgttaga tacacttata caaatattga
    14281 aaacaagtta aaagacttaa atttcaatga ttattttgca ggtttttcgc ttgaagagct
    14341 tacaaaagca attgttgatt aaaattttaa aaaatgaaag ttaacaataa acattatcgg
    14401 gcaatttggg tagaaaacga aacagttttt atgatagacc aaaacaaact gccttttgaa
    14461 tttaaaattt ttaaaagtaa aaacttgcaa actacttgca tggcaataaa aactatgatt
    14521 actcgtggtg ctggttctat tggtgctgct gcggcttatg ccatgcttca agctatttat
    14581 gaaaataaaa gccaagattt gtattcaagt ttagaaaaag ccaaaaaact aattgaccaa
    14641 actcgtccaa ctgcggtaaa tttattttat gcaacccaaa gagttttaga tgctgcaaaa
    14701 atttcaaaag aatttgcaat ttctgaagct catgccattg ctgacgaatg tgccgaacat
    14761 ggtcgcctga ttggagaatt tggaaacaca ttgatagaag acggtttttc tattgaaact
    14821 cattgcaatg caggttggtt agccttagtt gattacggtt ctgcccttgc tcctatttat
    14881 gctgctcata attctggtaa aaatatattt gtgtatgttg acgaaactcg tcctcgcaac
    14941 caaggagcaa gacttacagc ttgggagcta aaacaagaaa atataccaca tgcaataatt
    15001 cccgacaatg caggagcgta ttatatgtct aagggaaaaa tagatttatt tattgctggt
    15061 gccgacagaa ttgctgcaaa cggagatact gcaaacaaaa ttggaacttt agaaaaagca
    15121 attgctgcaa aatattatgg aattccattt tacattgccg caccaagcag tacaatagat
    15181 ttaaaaacaa aaacaggcaa atcaattcct atagaagaaa gaagtgcaga cgaagtaatt
    15241 tattgttcag gaaaattaaa atcaggaaaa attgaagaaa tgcaaattac aaatcctggc
    15301 tctggtgctt taaatcctgc ttttgacaca acacctgccg aactaattac aggaattatt
    15361 accgaaaaag gaattataaa accaaatgaa gaagaaatta ttaaattgtt tcaaaaatga
    15421 tgtttaaaaa agaaagaaaa gaagtagcaa aaataatgag aaggctttac gcacaaggtc
    15481 ttactacatc aagtggtgga aatgttagtt ttaaaaatga gaatggtttt gtttttatca
    15541 ctgccagcca aaccgacaaa tcctgcataa aatggaaaga tataattgtt tttgacgaca
    15601 ataaaaataa tattacgcct aagttaaagc caagcatgga aagtggaatg catttaaaaa
    15661 tttacgaaac acgtaaagat atttctgcaa tcgttcactc ccaccctgtt tatgcttcaa
    15721 gctatgcagt tgcaggaagt attccaaaca cagctttaac tggcgaagca agtttattac
    15781 ttggcaaaat ttctctatca gaatatgaat taatgggaac agaaaaactt gctgtagaag
    15841 ttgcaaataa actacaagac acagatgttt gcattatgaa aaatcatgga gcaataagta
    15901 ttggcaaatc tttattcgaa gcttttgata aaatggaagt tttagaattt gtgtgccatt
    15961 tgaatattaa ctcactgatt ttaaacaaag taaataaact aagcccagaa caaattcagg
    16021 aaataaataa aagtttttag tgtttagttg agaagttttt agtttttagt tgatgagttt
    16081 ttagtgttta gttaaattcc agaaagtttt aactaaaata aatcaaaaat ccacttttaa
    16141 ctttttaatt cctcgcttaa attacaacgt aagcctgcaa gttaaacaag tgtataaaaa
    16201 tataaattta tgtgcaataa acgcaaagag aaagcgtttt taggttatga aaactaaaaa
    16261 atttataaaa atagacctat accatatttg tgatttgtta gcaatcataa cactcatatt
    16321 tttagcggtg tttcttgtga acaaaacaaa attagactac tttctcttct ttgctggaat
    16381 tgcgtgctta acaacatcat tacaattgca ttttggacaa gctgccataa aaaacaagag
    16441 taacaaaact ttaaaagcca agcctgaaga tgaagataag gattttttaa taaacgtaga
    16501 gcctcactca aaagtttttc aaattgacgg tatcaaggtt gcaaacaaag tatataaaat
    16561 acccgacagt attcatgtga aagtaacaaa gaaaaacaaa gtacgtgtaa gttctttttg
    16621 tgcctatatg atttattggt tatataatgg cgggatatta gacgtagccc ctgacgaaaa
    16681 atggaataaa ctttttgagg cttgaaaatt atgtttacca aaaattttct tagtacgatt
    16741 tttggtaaac attttaacac ctatattttt tgctgattac acaatattgc acaatggtct
    16801 ataagtaaat tagcatattc aaaaccaaca tcagttattg ttttaacact tgtaccatcg
    16861 taacccatag ctaaatatgt tatataataa gtattgaaaa tgcaatcatg tagtttttta
    16921 tcacactgag ctacttcttt gtatttatcg actaagattc gtcctttccc ttccacatta
    16981 ggaaacgcag cctctaaagc tatcaagcaa gctttccata acctattgcc tgctcttttt
    17041 acacatttat tattttgata tgtttcggta gcctcataaa atttaccatt tttttctaat
    17101 atttttttgg ctttctcaac atactgcctt gctttttcta tagaatcttt ctttttcata
    17161 ttttaagttt taaagtgtaa tcctattttt tataacataa cagattcaat tatcaatttt
    17221 ctaaagagcg agtgcaaagc caaacttgtt agtgctatgc cgagagacga cagaaaatgt
    17281 agcgaagcta aattcttcaa ttatcaatta tcaattatca attatataat tctcaataca
    17341 aaaaagtagc cccaccagga atcgaacctg gatctacagt ttaggaaact gctattctat
    17401 ccgttgaact atgaggcccc ctcacaaaaa ttttcgcaca aatataaaaa ctttttttat
    17461 ttaagaagtc ttataaatgc aatttcgcaa caaacaaaaa taaaagctaa taaaacaaat
    17521 agttgccaca atggtttgcc gtctgcaaat ctttttaatt caagtttaaa atcgctttta
    17581 tctgagtcaa caagttttgc tttattatca aatttttcaa ctatgagttt atctaaatca
    17641 cttgcactta caaagtctaa aacagattcg cttctatcgt aatttagcga aattggcgaa
    17701 atatattctt cagttttata aagtgaatat gttccagaat ttaaaatttg ttgaggaata
    17761 tcaataatta tttttttgtt tgcatatcta tgtggcgtta aaatttcgaa atttgttttg
    17821 tctgtcaatt taaaaatatc tgagcttaaa atttccttat cggattttat ttctactttt
    17881 gctcccgcct taatcgttga atacaactga ttgcttatct gagagtttaa tgccatatta
    17941 taaaatgctg gagcaaaaat tgcagatttt acaaattccg aattgctttc atcaacatgt
    18001 gctgaaataa tataaatttt tccagaacca taatttccag cagccaacaa agttgcattg
    18061 ttttctaaat taagaacctt acgaacagaa gacgaagtaa atgtattgaa tttatgacta
    18121 tttgcaacta ttggcaattc gctttgcttt tcttttttga taaatacatt tttaaataat
    18181 tcgtgctcgt actctattga agcaattctt gtgttttgaa tactttgttc tgtaaaattt
    18241 cctgcattaa aaagatttaa aaaagaattt atcgaactga ctttcaattt tctatcagga
    18301 ataaaaatca aagagccacc gttttcggta tattccaaca aattggcact caaacctgta
    18361 gatatttctg aaaatgaatt taaaattacc aaattataat tattgaaatt ctcattttgc
    18421 tctgttccta atttaacttg ttttaaatca aaattttcag aatccgaagc ataaagagct
    18481 gttaaccaac gatttgactt ttcattattt attattaaaa ttttagtcat atccgaaacc
    18541 ttataattaa aataaagtat attatcataa attatcggat agtcagtaat ttcaagtctt
    18601 gcctcaataa atcctgattg agtatttaca tattcaattt caacgtcttt agtttcgcct
    18661 gcttcaatat taaatcctgc aacagcttta aggctgtcat ttatatataa ttgtagagat
    18721 tgctcaaaaa atgcttcgtc agaattattt actaatcttg caacaagagt ttccgacata
    18781 tttaatctat ggctaagcga ctcaaaccaa acagaatcaa taaaaatatt gttttgtttt
    18841 tcagctttta ggggaacaaa aattaaattt atattatcat ttaattttgc agaaatattt
    18901 tctacaaaag tttcttgcat gtctgaaata acatacaagt tcgcattttg ctcttttgtt
    18961 acattagcat tagcctttaa aaccacttcg tttaacgtat agcttgaagg acttatagaa
    19021 gttttgttaa tatttctcac aaaagcatct ctatcgctaa aatactgatg ttcaggcaat
    19081 aaatcatttg ttattaaaaa atattttgct gaacttgccg aactgtaagc aatttcttga
    19141 gcaatttgtt gtgcttgttc aaaaagttta ccttttttgc cggtattttc catagaaaat
    19201 gaattgtcaa gataaattat gtttaaaccg cttggtttat cattatttcc ttgttttatt
    19261 ggaataaaag gacctgcaaa agctattaca agcattgcta tagttaaaat tcgagcaatt
    19321 agaattaaaa tttgtttaag tttagttttt gttttggttt ctttgcgaat attttctaaa
    19381 aagcgtgtat tactaaaata taaagtttta tgtcgcctaa aattaattaa gtgaataatt
    19441 atcggaacag ctattgcaaa catcgcccaa aaaaaggcag gattaataaa actcattttt
    19501 taaagtttcg ttttaaattt tggcaaagtt aaaaaaatat cggttaaaat aggaaatgaa
    19561 atagaaatgt tgtagttttg ttttgcaaat tagattttag ataacataaa agctataacc
    19621 ataaaaaagt aatgaattat ggcaaaaatt aacgtaaaaa atactgaaat cacaattata
    19681 tctattgagg agaaagatta catttctctt accgatatgg caaacgcgaa agaaagcgaa
    19741 agccgtgcag cagatattat taaaaactgg ttgagaaatc gttatacact tgaatttttg
    19801 ggtacatggg aacaaataca taacccaatt tttaaagtgg tcgaattcga ccactttaaa
    19861 atgcaagcag gattaccaaa ttttgtatta agtgtttctg aatggataga aaaaaccaat
    19921 gctattggga ttatcgtaaa aaaaggcaag tatggaggaa cttttgcaca taaagacatt
    19981 gcttttgagt ttggctctgc aattagtgtt ccttttaagt tgtatttaat aaatgaattt
    20041 caaagactta aagaagaaga acaaaaacaa cttggttggt ccgcaaaacg ggagttagct
    20101 aaattaaatt accacataca taccgatgca attaaacaaa atctcattcc acaagagctt
    20161 tcaacgacac agacatcaat aatttatgcc aatgaagccg atgtgttaaa tgttgctttg
    20221 ttcggaatta cagccaaaca atggcgagac tcaaaccctg aattgaaagg caatattcgt
    20281 gattatgcaa caataaatga attgatttgt ctgtcaaata tggagaatct gaatgcagtt
    20341 ttcattaacg aaaaaatgcc ccaaaaagaa cgtctcataa aactaaatca aatagcaatt
    20401 cagcaaatga gtattttaca agaagttgaa aatcgaaaat tattaaagta aaagaagtta
    20461 aaatagaaaa tgaaataaga atgttatacc tttgttatgg aaattaaaag ttggtaacaa
    20521 gaaaaaaatc tgagtaaaca attgaagtca gatagatttt tattatattt gcaatatgaa
    20581 tacaagagat gtcataataa gtaagatttt ggaactttca aaggaaaagt atccagatgc
    20641 tgaaatttat ttgtacggtt ctcatgcacg tggagacgca aaaccttttt ctgattggga
    20701 tttattaatt ttactaaatt taaagcaaat tccttttgct ttagaaacta aaataatgga
    20761 tgaattctat gaaatcgaat tagaaacagg tgaaatttta tccccattga tatacccaaa
    20821 agaagaatgg aatgtaaaat atagacaaac tccactttat gaaaatatta aaaatgaatc
    20881 aattagatta atatgacagg catacagaag aaaaggttat gccttattat gactttgtta
    20941 agaaattcat actacaaata gaaaaaatca taaataacta attgccagtt gccaacaaga
    21001 ggtgtaggac ttaaagttca gttaaaagtt aaaagtgcgt ctgagagaca attctaagtt
    21061 gaaaataata tgcaattttt tgttttgtag tggaaaatgt tgtatatttg ttatgtaaaa
    21121 cattcgttag caaaaataaa tatgaaaaag atattaatta ccttagtttt atctattcta
    21181 tttttaaatt ctattgctca agagcttgaa ctaaaaaaga aatggagttt aggattttca
    21241 tatactccaa acatttccta tttatataat ccaacagtat attttggtgg tcaagttcct
    21301 acaggataca gttaccagtc aaagaaatct gtaagagacc ttcctatatt tgagtttatg
    21361 aattttggga taagaatttc tgctgtatta aatgaaaaga atgagataga aacaggagta
    21421 attttaagta ataaagatta ttattctcca caaggattga tacatgatga aatgccattt
    21481 gcgcgtttta aacctagagc atattttata gatgttccgc taatctataa acacaacata
    21541 tctaaaattg gcaactataa tttgaattta ataggtggcg ttataggaag tgttccattg
    21601 tataaacaca ttgattttcc aggtgaactt tatctttatg caactgatgg taatgagttt
    21661 gcattgacac aaaatttaga cttttatcca ctttgcatga attttaatct aggtcttaga
    21721 cttctcaaaa ataaaatcaa taatattcaa tttgagtttg gtcctgtttt tcaatctgca
    21781 attatgccat ttagtcgaaa aattgaagga aacataatca aagtaagagg aaaccacgaa
    21841 ctaattgttt ctaaccctga aaaaggttat atgccgtgtt tcattggctt tgatttaatt
    21901 ctacgtttta atttttttga tttaaccaaa tataaataag atttgccaac aacagcttgg
    21961 tttaatagct ggttttaatt tttcaaataa atttttaaag tgcaaagtaa aacctattgc
    22021 tacattttcc agctaaatat acgcatacga ttttttttac actaatgttt gtatcttaaa
    22081 aaatttatgt acatttgcgt attaaacaaa aattaaaact actgatatga aaaaaattat
    22141 tatccttttt gtaaccatta gctttacctt aatcccaaac cctaaaaaat taggtaattt
    22201 aaattgaaaa aaaaacatat aaatactt
//
LOCUS       DAOPNX010000038        21846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_2987_length_21846_cov_5.604470, whole genome shotgun sequence.
ACCESSION   DAOPNX010000038 DAOPNX010000000
VERSION     DAOPNX010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21846
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2987_length_21846_cov_5.604470"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PKN53_05715"
     CDS             <1..704
                     /locus_tag="PKN53_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88480.1"
                     /translation="VIFTILGVIGGFIHFYCSKEVYKTKLIASSPIVDNRIVYELLEP
                     LKIHIKNNQNDSVANKLNINSDVAASIHALSFDTTVNRTLIVNLEVFSKENIKVICNG
                     IIDFLNDIEYINRQVNYKKKELTDYIAIIDNEISKLNNLQDAMLKNAQLASNLSISNT
                     YSEMLTLVERKRELEKELSGLTSFQIINANIVFDTSKSSVIKSLLVFTFLGAFLGFCI
                     AIFIDLKRKVNAINK"
     gene            complement(729..1874)
                     /locus_tag="PKN53_05720"
     CDS             complement(729..1874)
                     /locus_tag="PKN53_05720"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOD88481.1"
                     /translation="MADYIKNLKTEISKYLKTDNIYFHYKGRVSLYVILQAMNVKEGD
                     EVIIPAYTCVVVPNPIIYLGAKPVYVDIDKDNLCMDINLLEKNITEKTKVIICQNTYG
                     LSANVDTIVEIAKKHNLYTIEDCTHGFGGFYKNQPNGSYCDAAFYSMQWNKPFSSGIG
                     GFLTINNAELLENINKIDRQKAKPGFLTKLNLKLLFFAKRYFLNNTTYWFLLNFYRWL
                     SKNNLVTGSSSGNEISDIKMPDNFLMDYSQTQAKEGLRNIKKLPKLLALRKNNALKYT
                     EFLLKNNKVFVKKDLFENHSFLKYPILVKNRDKFFDLARKHKIELGDWFNSPIHPVQN
                     NFEKWEMYPEKYPIANYTSQHVVNLPTDTKKIKKVLSFMEKFIDEIL"
     gene            complement(1876..2883)
                     /locus_tag="PKN53_05725"
     CDS             complement(1876..2883)
                     /locus_tag="PKN53_05725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD88482.1"
                     /translation="MSNLVSIIIPTYNEEKNIRECLNSVLEFNYPLNSLEIFVVDGMS
                     EDKTREIVLNEFCALHPNIRLLDNPKRTAPFAFNIGIKESNGDYIVIIGAHSSYSKDY
                     IKNLISWHKKLDAANIGGVMQTEVRNKNKTTSAIIKVLSNKFGVGNANFRTGSKKVVA
                     VDTVAYGCYKKECFDKFGLFNEKLTRNQDIEFNKRLIKGGEKIYLVPNATCTYYARET
                     FSGIAKNNFQNGMWNILTVFYTKDFKSLSLRHFVPLFFVLALLLPLILALFYWQFVFV
                     SALALVLYLLLIISVSIKLNDKSTSFLNLVFTFFTLHISYGTGSLIGILKLPLLAIKK
                     E"
     gene            complement(2889..3986)
                     /locus_tag="PKN53_05730"
     CDS             complement(2889..3986)
                     /locus_tag="PKN53_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOD88483.1"
                     /translation="MKICHISTVHPRYDVRIFLKECVSLSKSFKEVHLIIADGLGDEK
                     KNNVQIHDIGKPKSRKDRFLNFNKLALSKAKELQAEIYHLHDPELLRIALKLKKIGAK
                     VIYDSHEDLPRQILNKTYIPKYLRKLISRIIENYENKISKKINGIAAATPHIRDRFLK
                     INKNTVDINNFPIIEDIKFNSDWENRELAIGYIGGIFKTRGIFETLDAINNTDIKLHL
                     AGNFSHSQLETECKSHPAWKQVEFHGYLDRNGVNELLKKVRLGMVILEATPSYIVSLP
                     IKMFEYMAAGLPVVASNFPLWKSIIEENNCGICVDQTNPEEIRNKLLALISNDDKLIE
                     MGKNGRKAVELTYNWEVEEKKLVEFYKNLNN"
     gene            4117..5391
                     /locus_tag="PKN53_05735"
     CDS             4117..5391
                     /locus_tag="PKN53_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOD88484.1"
                     /translation="MFRQISKIISPKSEFSKNFFILFKGTMIAQIIPMLLMPVLTRMY
                     SPDDFGVLELFVSVTTILGSIANLRYELSIVLPDRKEDAWNIMSLGFLIAFVLSIILQ
                     ILIGFFADTIAGLLNNSQIKFWLYFVPITVFLQGAYNMLSYYNTREKSYKNISSASIW
                     RASTRTGTQIGLGFLSKTPIGLIVGQIIGYLVSIFPLMSKIKLKLFIKKTSWSNMKMQ
                     AKRYVDFPKYQMPATLANVLAVNLVSVLISMLYNVTQVGYYSLSNRILGLPSAVIGTS
                     MQNVYYKEAHEQMKKYGHAKEVFWSTLKKLSLMAIPIIVVIFFLGEWLFALVFGEEWR
                     IAGYYAKILIPLIMIRFVTAPLSISLSVFEKQKVALYWQIGLLVLTLLVFGIVKIFDW
                     DMISFMILLSSVLSLYYLYFLYLLYRVVARKL"
     gene            5479..6813
                     /locus_tag="PKN53_05740"
     CDS             5479..6813
                     /locus_tag="PKN53_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOD88485.1"
                     /translation="MIKIFIQNKFVAEKKYVLNYIFGEIYNTEYEILTDNEPLYRFVS
                     DNYRLIIDDVFFSNFEDGYKYFQNIINIPTDVDFMTCEGFSFAIPILFGKDELRNEDG
                     LIYCGNDIIAAIFFMLTRWEEIANKTKDKHDRFDEKENLSIKYGFHKRPIVNDYISVL
                     AKMLNDAGIEVAPSQRDFRLFLTHDIDFFARYDKFGKIVKALLGDIFRRKNFKIFFNT
                     LKTVYKQIFKKAPDVYDKFDFFMDISETKNVKSEFYFIPGLKGEEGITYSILDKKIKK
                     TIHNIKERNHIIGIHPSYSSYNNVSQLKREVNRLYNLRIDVSQGRQHFLRYLIPQTFR
                     DWEVCNLKIDSGMGFNSNIGFRCGICYEFPLFDVELRNQLQLRERPLIVMDKALRARC
                     ENQQDCVNESVRLANIVKSYNGDFVLLWHNSNLVVNEWEGWDSIYKEIVEGI"
     gene            6992..8077
                     /locus_tag="PKN53_05745"
     CDS             6992..8077
                     /locus_tag="PKN53_05745"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOD88486.1"
                     /translation="MSFVFLILFYLWMLLSLIWTPSESYSYKKVILFIPNIIGFGFPL
                     LCKRFDIAKFFRIVSITLIFVSIWFLKIYLDYLDDGYSKKTYYEPILGLYLSCASFMG
                     LNILVILGAKKRIFKNLFVSSFVVTLTIILLFLLGARGPFIFVLLLLIPLFIIKYIYS
                     YYYNKVLLSDFKKALYSGLTVLLFFVFFLIFEKEVKVLLDRSVFRISLLMPSSDSETS
                     SVPTTSNTTKSSSINTSVDTRIDQLKFSMDLIKNNKFESIYGFGVGSFGILYLQKDFR
                     AYPHNIFLEIWVELGLIGLILFLIFLYFVFSKNLNGTNYINLLVLVYIILNVLKSSSY
                     VDIRVFFAVFAMYMLSSNDKSLNKVAN"
     gene            complement(8085..8657)
                     /locus_tag="PKN53_05750"
     CDS             complement(8085..8657)
                     /locus_tag="PKN53_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88487.1"
                     /translation="MKKIFILFVLSIFTLPLFSQVEEPVDNPGGGGFGAILLNFRSSD
                     ENFALYTGGGGGFVVKDFRIGAFFSGMAKSFTKNDSIVSSMKIGCTQGGLWFAYPFFK
                     KHALHGLAEMKFSIGNTKVIDTNWQHHDKKVFWGFNPSLAIEYDISEVLKIAAGVEYQ
                     YSYFRKLPTLYSTDAFSSPGVFISVKLGTF"
     gene            8752..11361
                     /locus_tag="PKN53_05755"
     CDS             8752..11361
                     /locus_tag="PKN53_05755"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOD88488.1"
                     /translation="MKTTLFVYLLLLSGFLFSQSVLSPALVSELGKHEGYYINVNIYF
                     QSHYDINELARNLDAKKASFDFRTKSVNDLLNKNAEISLSEFAVCLDFILNQDSRAVG
                     KIKFFRAANALNIEIKPNFVYYLADQKSIRFIDINSARYKIIGTERVLSSEPKLVGGT
                     EPGLKAINAHKLWEKGYTGRNILFLSMDTGVHATHPAISDNFAGNYLPLSQCWYGIRN
                     PKPVDNAGSSHGTHTTGTVLGLDKTTNDTIGVAFNAKWIASDPVASSDSDLLTPADFM
                     AVFDWVLNPDGDETTSDDVPRVINNSWGYNYDLALNFGACDLPEAEILVTIETAGICS
                     PFSAGNEGPGAGTTGFPAMLAYNIVNPMSIGAVNGNSENFPIADFSSRGPSLCVEEEG
                     RLKIKPEVVAPGVSVRSASGASSYSYLQGTSMACPHVSGALLLLAEAFPMASAYELKL
                     ALYETAIDLGETGEDNIYGNGMIDALAAFDYLSNIYTPAPPITNEYDLSLELTSSEKS
                     FVCQDEKFLNTQMKIKNLGENPINGFNVKVYLNEVIVCDSTINESIVEGSSFEFLYPN
                     LELSAGENYIHGIVKPLEDLSEYDRFNNGFNHRVFLLSEVDSSYYQNFENISNLDNSE
                     FVIINPDYRTTWSMLNWGENDEHKAIGVNFLEYTPKNWNIDDLYLPEITMPAENDSMF
                     LSFTYAYKRRTLNLYKDSLIIEVSTDCGLSFDKILFAEGGENLATVEGNSNYKFYKPI
                     EASEFDTINISLNEFKGEKLVIRFRTKNDNGSVLYIDEMKIGVLDNSSIYSNKLVVQE
                     PEIYPNPTSDIIFVKIYESEEFVNIYDLTGRLVLNQKVYNEISKVNLGDLTEGVYFIH
                     FINSGKTKKLIKK"
     gene            11461..12351
                     /locus_tag="PKN53_05760"
     CDS             11461..12351
                     /locus_tag="PKN53_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88489.1"
                     /translation="MKKVFVISVLMMIVGLALQSCGPEDTHGPKIYILNPDGKILHGE
                     AKDTTLLLWSKYVDPGVFVEDNASKTEDIVVTLDTTVLPLNKEGYLRATRGTVEEMLN
                     LTYTATDLAGNVSTNVRPFRVANVSEVFAGSYLISRTAMHVNDTNYTSTISADARVAG
                     RLRFNKVYAHRWSGKNTYFKVNADLYSSELSPASFDERYGYLCKKGDPGVCYYSGLTY
                     TPAKEGIYDYYYLKIDAQNYEDSLGNVVYIRGIEDVNDLPLSKIEFLGDTRTITKIIL
                     ELNVTKNGQVDKVKEVYIPR"
     gene            complement(12408..13181)
                     /gene="dapF"
                     /locus_tag="PKN53_05765"
     CDS             complement(12408..13181)
                     /gene="dapF"
                     /locus_tag="PKN53_05765"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOD88490.1"
                     /translation="MLLHFSKYQGAGNDFIIINMMNNKIELNQKQIEFLCNRRFGIGA
                     DGLMLVLKNDDYDFYMQYFNADGKEGSMCGNGGRCIAKYAYLNKISSSEVNFLAIDGK
                     HKANIIRSSVSLSMSNVNKIQDFEDGYFLDTGSPHFVNFVNDLNSINVKQEGRKIADE
                     SRFSPMRTNVNFVEIKNNNIRIATFERGVEDETLACGTGSVASALALHHSGKIKSNEI
                     VLIAKGGRLKVNFEYNTNCYQNIILSGDAEHVFEGEINI"
     gene            complement(13230..14426)
                     /locus_tag="PKN53_05770"
     CDS             complement(13230..14426)
                     /locus_tag="PKN53_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88491.1"
                     /translation="MNKFVFVVCGGSEHIEKLNFSLKFLRKYSKNEIIVLSDLKRNEI
                     KIEHNNIIQIDTPEDFDHHQASIFIKTGLNKFLPKTHNYCYLDGDIVAINEKVDEIFN
                     FYKPPITFAKDHCSLQYFSPHAMNCSCIVNKIKNDETFKIKISQLFEKIDFSNPKIKA
                     QSQQLLQIFAEYQNNPIKYSIQNITYFIKRYILPIKQFKLKQFKFLKSDKCWYNSDNE
                     AILFDYPYYEKKIWGKTGIIFNFEENLWENRKGEKYEFKAPSCSHLSEYLQKEYGIKI
                     PDNWRHWNGGVFLFNDKSEEFLNFWHEITIKEFSNPYTKTRDQGTLALSVWKFGLENH
                     PNIPEEFNWICEFANKDIDWDKNLGYTKDGFKTHFYPKLLHIYHEWGNKNWSIWQSVA
                     NQNKNT"
     gene            complement(14440..16221)
                     /gene="asnB"
                     /locus_tag="PKN53_05775"
     CDS             complement(14440..16221)
                     /gene="asnB"
                     /locus_tag="PKN53_05775"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOD88492.1"
                     /translation="MCGITGWWSKNSKINRELFNSMRDTLTHRGPDGYDSYFSSDEKL
                     ALGHRILAFNNFSKSDTQVTCSENKNVYSVFNGKIYNYKKLRILLEEKSHIFKSNSDD
                     ELIIHAYQEWGMKFLEKIDGMFAFAIFDNKLQKLFLVKDRFGVKPLYYFCDKENLIFA
                     SEIKAIIKNPEIKREINKKSISEYFIYRYIPSPNTIFQDIFKIPPAHYIEIDKNFNIS
                     HKKYFELKIDIKKQRKSKLIKEIEELIRNSIKEQIDSDTDIASFLSGGFDSSILVKYF
                     SEIKKEINTFSIGFENWEKSEHLFAEKVSKIYKTNHFSKLLNQESLNILDELMYYYDE
                     PIADISIIPTYFACKLASQHNKIILSGEGADEFFAGYGWHKEYLWEISKAELTKAKKW
                     NWDLPINNFDIDSYAKAMAMGEFDEKELKFLLTEKYHQYTPTDTKEFYRKYFNENIDK
                     PKRFQAIDIKAFMGELLLTKMDRASMANSLEVRMPFLNKEICEKMLSLDTEKYFDKKK
                     QKIILYKLLQKDLPKKILNRKKQGFTGPDEFYMNFEFYEKNLNNSKLVEAEIINQSAI
                     NHYIKQKDHWRLWKILVLEKWFKKWIA"
     gene            complement(16193..17167)
                     /locus_tag="PKN53_05780"
     CDS             complement(16193..17167)
                     /locus_tag="PKN53_05780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD88493.1"
                     /translation="MKNESDTMLNIGILCHNHFNFIEKCINSILCQKTSYNYNIIIVD
                     DFSTDGTREILENFFQQNPNKIELIFNNENIGALNSAKILAQKAKAKYFCFLDGDDYW
                     CYENKIQTQIDFLENNPDYAGCFHDAKIEQYNKSNDANYNHRTHINWKTYSQFNRYVE
                     DFMPWDLLVRNIIPTASLIFRNTKVSKFLNNFNASALSLSWALHLEIIKNSKLKYFNE
                     IWSVYNDHPKGASKIHDITVFKRNNVKILENLLKDPEWKFYKYEIYDTICKEYRSMLK
                     SKWELEKSWKEYKKSLNAYKKYIRIANKFDIDQLKNDFNYVRNNGLVE"
     gene            complement(17168..17410)
                     /locus_tag="PKN53_05785"
     CDS             complement(17168..17410)
                     /locus_tag="PKN53_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOD88494.1"
                     /translation="MENIKTDLINILKKYTLDKNVWNDFNDNSSIISDLKINSARIVD
                     IVIDLEEKYDIEITDEELEKLTCFKDIVKLIDSKIN"
     gene            complement(17429..18706)
                     /locus_tag="PKN53_05790"
     CDS             complement(17429..18706)
                     /locus_tag="PKN53_05790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOD88495.1"
                     /translation="MSKRVVITGLGVISPIGNDIESFKLSMQKGISGIKYIKELADYN
                     FASQIGGVPDINNSEYEKIFEKFELKDADLTVKYSVLAALQAWNDAKLELPDKFAENI
                     NENYGAVIGSCAGGAELYARKLYPLVNSKNIRKLGSQIIENLMHSGTTAVLSNIFALA
                     NQTISNSSACATGAESIIMASEKIKQSKASLFIAGSADPYSPYMWAGFDSMRLLARKF
                     NDKPTQASRPLSASADGFVASAGAGMLIVEELNHALNRNAKIYAEIVGYEYNSGGQRN
                     GGSMTASNPKRVIDCIKSALNNANISGKDIDLICGHLTGTKFDYQEVENWKTALNLNE
                     KLPYINSLKSLLGHTIGAAGAIETIAAILQLQNNFIHPSINCEDLNENIKTIWNKNKI
                     PQKLIKDINLNYIAKASFGFGDVNSCIILKKFN"
     gene            complement(18699..19160)
                     /locus_tag="PKN53_05795"
     CDS             complement(18699..19160)
                     /locus_tag="PKN53_05795"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88496.1"
                     /translation="MHIETNQDYLFSSIISKLPYGKDFLFVDEIQYIDKDKIVGTYKF
                     KSSAYFYNSHFLHKPITPGVILIEAMGQIGMVSHLIFLNDLHLSNKNFMPLFSNLESS
                     FYKIVERGEKIKVIGDKIYFRNNVLKSEIKLYNKLEELCVVCKAQLSLKYE"
     gene            19240..20361
                     /locus_tag="PKN53_05800"
     CDS             19240..20361
                     /locus_tag="PKN53_05800"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD88497.1"
                     /translation="MVNSKFHTLYFEATRNCNFSCIYCSTGSSKSKKCTDADYQAIVD
                     HIFVPAKNLGTKFIDFSGGEFLLRNDSLKLLQTAINMGFSVGIVSNGTTLNNEKLQQL
                     KDIAGEELIISLGINSFDSDNIVTRDNHYTNTIKLIEKISGFGFKINISVTIGEFNKK
                     SFADTIKKISDMSLAFNRIPFSIRNCNALDLMITPDSMKNYFHPVLRNYFNGQISYVP
                     FFLPPDSYNTITKSNIYNDELPLNPSIGCWVGSYYSINPEGEVSPCPLFGDQISGGNV
                     FKENLKDILFESELFKKILNRKNFKGKCGNCKYTLTCGGCRVMAYFLNGDPYAEDPIC
                     FIDKLSNEEIEAIEKETIKSFKNYNRMIKIRNLLINKDS"
     gene            20358..21140
                     /locus_tag="PKN53_05805"
     CDS             20358..21140
                     /locus_tag="PKN53_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOD88498.1"
                     /translation="MNTNKEFILIIGGSGSIGLACAKMLAKKKYNLVLIHRDSRSMQK
                     LSQVEFDKIRNSGIELYNINVNANTDEAFIKLKELLSENNIKSIKAFIHAYADANIGS
                     ILESEKELSADDFIHSFNSMALSFVTWSKWLKQNNYLQNFSKIIGFTSEGSFRVLNNY
                     VAVGMAKSALESACKYLAIEFAPYEINVNLINSGIVFSKSVELMEKQKPFIEKAIKRN
                     PFKRLTEPSDIAKVVSFLISDDSNWITGQKITVDGGEQLLSI"
     gene            21171..>21846
                     /locus_tag="PKN53_05810"
     CDS             21171..>21846
                     /locus_tag="PKN53_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88499.1"
                     /translation="MNKNVILLFYPLVDKDKQIPNLPFSVLNLERMLRDLGLEIIIID
                     ERVIVDYKQIILENKDRLLMAGVSAMIGYQMISGKEFSLFFKQHSDAPLVWGGWFANV
                     LPELALKENFIDFIIHGQGEIPFKNFVETFIRGEDFKNIRGLGYKIDNKIVINPIENI
                     VDEKSFPNINFNLVDVNKIANINEPDNTGASSLNYIATIGCPYSCAFCCLASIWGQKT
                     FNKDVSL"
BASE COUNT         7380 a         2843 c         3256 g         8367 t
ORIGIN      
        1 tagtgatatt tactatacta ggagtaattg gaggatttat tcatttttat tgttctaaag
       61 aagtttataa aacaaaactt attgcttcaa gtcctatagt tgacaataga atagtttatg
      121 aattgttaga acctttaaaa attcatatta aaaacaatca aaatgatagt gtagccaata
      181 aattaaatat taactctgat gtagcagctt caatacatgc cctttctttt gatacaacag
      241 taaatcgaac tttaatagtt aatttggaag tgttttcaaa agaaaatatt aaagttattt
      301 gtaatggaat aatagatttt ttaaatgata tagaatacat taacagacaa gtaaattata
      361 aaaagaaaga attaactgac tatatagcaa ttatagataa tgaaatttca aaactaaata
      421 atttgcaaga tgctatgttg aaaaatgcac aattagcttc aaatctttca attagcaata
      481 cttacagcga aatgttgact ttggttgaga gaaaaagaga gttagaaaaa gaattatcgg
      541 gtttaacaag ttttcaaatt attaatgcca atattgtttt tgatacttct aaatcatctg
      601 ttattaaaag tttgttggtt tttactttct taggtgcgtt tctaggtttt tgcatagcaa
      661 tatttattga tttaaaaaga aaagttaatg caataaataa gtagtaataa attttgaact
      721 atagaaattt ataagatttc atcaataaat ttttccataa aagacaaaac ttttttaatt
      781 ttttttgtat ctgttggaag attaactaca tgttgtgagg tgtagtttgc aataggatat
      841 ttttcgggat acatttccca tttttcaaag ttgttttgaa ctgggtgtat tggagaatta
      901 aaccaatctc caagttcaat tttatgtttg cgggctaagt caaaaaattt gtctctattt
      961 tttactaaaa taggatattt taaaaaagag tgattttcaa ataaatcttt ttttacaaaa
     1021 actttattgt tttttaataa aaattcagtg tattttaagg cattgttttt tcttaaagct
     1081 aataattttg gtagtttttt tatattgcgt aatccttctt tagcttgggt ttgcgagtaa
     1141 tccattagaa agttgtctgg cattttaata tctgaaattt cattaccaga cgaagagcca
     1201 gtaactaaat tatttttgct tagccatctg taaaaattta acaaaaacca atatgtagtg
     1261 ttattcagaa aatatctttt agcaaaaaac aaaagtttta gatttaattt tgttaaaaat
     1321 ccaggtttag ctttttgtct gtctatttta ttgatatttt ctaatagttc agcattatta
     1381 attgttaaaa atcctcctat gccactcgaa aaaggtttgt tccattgcat tgagtaaaag
     1441 gcggcatcgc aataacttcc attgggttgg tttttataaa atccaccaaa accgtgtgtg
     1501 caatcttcga tagtatataa attatgcttt tttgcaattt caactattgt atcaacattt
     1561 gcagagagtc cataagtatt ttgacagata ataacttttg ttttttctgt tatgtttttt
     1621 tctagaagat ttatgtccat acataaattg tctttgtcaa tatcaacata aacaggcttt
     1681 gctcctaaat atattatggg atttggaact actacgcaag tataagctgg gataataact
     1741 tcatcacctt cttttacgtt cattgcttgt aaaataacat aaagcgaaac tcttccttta
     1801 tagtgaaaat aaatattatc tgtttttaaa tatttagata tttctgtttt taaattttta
     1861 atataatcag ccattttatt ctttttttat agctagtaaa ggtaatttta atattcctat
     1921 caaagaaccg gtaccatatg aaatatgtaa agtgaaaaaa gtaaaaacca aatttaaaaa
     1981 gcttgttgat ttatcgttta attttattga aacactaata attaatagta aatataacac
     2041 caatgctaat gcacttacaa aaacaaattg ccagtaaaac aaagcaagaa ttaaaggaag
     2101 taataatgct aatacgaaga ataaaggaac aaaatgtcta agtgataaag atttaaaatc
     2161 tttagtgtaa aaaactgtta aaatattcca cattccattt tgaaaattgt ttttagcaat
     2221 acctgaaaat gtctctctag cgtaataagt gcatgtggca ttagggacta ggtaaatttt
     2281 ctcgcctccc ttgattagtc ttttattaaa ctcaatatcc tgatttcgtg taagtttttc
     2341 gttaaacaaa ccaaatttgt cgaaacattc ttttttataa catccataag caacagtatc
     2401 tacggcaact acttttttgg aaccagtcct gaagtttgca tttcctacac caaatttgtt
     2461 gctcaaaact ttaataattg ctgacgttgt tttgttttta tttctaactt ctgtttgcat
     2521 cacaccacca atattagctg catcaagctt tttgtgccat gatattaaat ttttaatata
     2581 atctttacta taggatgagt gcgctccaat aattacaata taatcaccgt tactttcttt
     2641 tatgccaata ttaaatgcaa atggagctgt tcgttttggg ttgtcgagaa gtctaatgtt
     2701 tggatgtaat gcacaaaact catttaaaac aatttctctt gttttatctt cactcattcc
     2761 atctacaaca aaaatttcga gactattcag tggataatta aattcgagaa cagaattcaa
     2821 gcattcgcgg atatttttct cctcgttata agtgggtatg attatagata ccaagttact
     2881 catagttttt aattgtttaa atttttataa aattcaacca atttcttttc ttcaacttcc
     2941 caattgtaag ttaactcaac agctttgcgt ccgtttttgc ccatttcaat tagtttgtca
     3001 tcatttgaaa ttaatgctaa tagcttgttt ctaatttctt caggatttgt ttggtcaaca
     3061 caaattccac aattattttc ctcaattatg cttttccata aaggaaagtt tgacgcaact
     3121 actggtaaac ctgctgccat atattcaaac atttttattg gtaacgaaac tatataacta
     3181 ggtgttgctt ctaaaataac cattccaagt ctaacttttt ttaataattc atttactcca
     3241 tttctgtcta ggtaaccgtg aaactcaact tgtttccatg ctggatgtga tttgcactct
     3301 gtttctaatt gagagtgtga aaaattacca gccaaatgaa gttttatatc tgtattattg
     3361 atagcatcaa gagtttcaaa tatgcctctt gttttaaaaa tgccaccaat atatcctatt
     3421 gcaagttctc tattttccca atcagagtta aattttatat cttctattat aggaaaatta
     3481 ttaatatcaa cagtgttttt attaattttt aaaaatctat ctcttatatg tggagttgca
     3541 gcagctatgc cgtttatctt tttagaaatt ttattttcat aattttctat aattcttgaa
     3601 ataagttttc ttaaatattt aggaatatat gttttattta aaatttgtct tggtaaatct
     3661 tcgtgtgagt cataaataac ttttgctcct atttttttta gctttaaagc tattctaagt
     3721 aattcagggt catgtaaatg atagatttcg gcttgtaatt cttttgcctt acttaatgct
     3781 aatttgttaa agtttaaaaa tctatctttt cgtgattttg gttttccaat atcatgtatt
     3841 tgaacattgt tctttttttc atctccaagt ccatcagcaa taattaaatg tacttctttg
     3901 aagctttttg atagagaaac acattctttt aaaaaaatcc ttacatcata acgaggatga
     3961 actgtactta tatggcaaat cttcatgttt ttaactagaa aatattattt gacaaaaata
     4021 tataaataag tttagaatta aaattcatat tctgttttgt gaaaaaattc ttgtttattg
     4081 ctacgattaa ttatttttgt gaaatatttt taaaagatgt ttagacagat ttctaaaata
     4141 atttctccaa agtcggaatt ttcaaagaat ttttttattc tttttaaggg gactatgata
     4201 gcacaaatca ttcctatgtt gttgatgcct gtgcttacac gcatgtattc tccagatgac
     4261 tttggtgttt tagagctttt tgtttcagta acaacaattt tgggaagtat tgcaaattta
     4321 cgttatgagc tttccattgt tttacccgat agaaaagaag atgcatggaa tataatgagt
     4381 cttggttttc ttatagcttt tgtgttgtct ataattttgc agattttaat aggctttttt
     4441 gccgatacta tagctggtct gttaaataat tcacaaataa aattttggtt gtattttgtc
     4501 ccaataactg tgtttttgca aggagcatat aatatgttaa gttattataa tactcgcgaa
     4561 aaatcatata aaaatatttc atctgccagt atttggagag cgagtaccag aactggaact
     4621 cagataggtt taggcttttt gtcaaaaaca ccaataggtt taattgttgg acaaattata
     4681 ggctatcttg tttctatatt ccctttaatg tctaaaatta aactcaaatt atttataaaa
     4741 aaaacctctt ggtctaatat gaaaatgcaa gcaaaacgat atgttgattt tccaaaatat
     4801 caaatgcctg caactttagc caatgtattg gctgtaaatc ttgtgagtgt tctaatctct
     4861 atgttatata atgttactca ggttggatat tattctctgt caaatagaat tttaggacta
     4921 ccaagtgcag ttatcggaac ttctatgcaa aatgtgtatt acaaagaagc tcatgaacaa
     4981 atgaaaaaat atggacatgc aaaagaagtg ttttggagta ctttaaaaaa acttagcttg
     5041 atggcaattc caataattgt agtaatattt ttcttgggag aatggctttt tgctttagta
     5101 ttcggtgaag aatggcgtat agctggttat tatgcaaaaa tattaattcc tttaattatg
     5161 ataaggtttg taactgcacc attgtcaatt agtttgtcgg tttttgaaaa acaaaaagta
     5221 gctttatatt ggcaaattgg attgttagtt cttacattgt tagtttttgg aattgttaaa
     5281 atttttgact gggatatgat aagttttatg attttgctat cgtctgtttt gagtttatat
     5341 tatctttatt ttttatattt gctatataga gtagtggcaa gaaaattata ataaatgcaa
     5401 aactttaaac ttaaaagaaa aaagtttgtg cagaatttta atactttgct atatttgtag
     5461 aaatttcata cacaaattat gattaaaatt tttattcaaa ataagtttgt cgccgaaaaa
     5521 aaatatgttc tgaattatat ttttggtgaa atttataata ctgaatatga aattttaaca
     5581 gataatgaac ctctttatag atttgtttcg gataattata gactaataat tgacgatgtg
     5641 tttttttcta attttgaaga tggctataag tattttcaaa atattataaa tattcctaca
     5701 gatgtggatt ttatgacttg tgaaggtttt agttttgcaa ttcccattct gtttggcaaa
     5761 gatgaactta gaaatgaaga tggtcttatt tattgtggaa atgacattat agcagctata
     5821 ttttttatgc ttactaggtg ggaagaaata gcaaataaaa ctaaagataa gcatgacaga
     5881 tttgatgaaa aagaaaatct ttcaataaaa tatggttttc ataaacgtcc tatagtaaat
     5941 gattatataa gcgttctagc taaaatgtta aatgatgcag gcatagaagt tgccccttcg
     6001 caaagagatt ttcgattgtt tttaacacat gatattgact tttttgctag atatgataaa
     6061 tttggaaaaa ttgttaaggc acttttaggt gatatttttc gtagaaaaaa ttttaaaata
     6121 ttttttaata ctttaaaaac agtttataag caaattttta aaaaagcacc tgatgtttat
     6181 gataaatttg atttttttat ggacatctct gagacaaaga atgttaaatc tgagttttac
     6241 tttataccag ggttaaaagg agaagagggt attacttaca gtattttaga taaaaaaata
     6301 aaaaaaacta tacataatat aaaagaaaga aatcatataa taggaattca tccttcatac
     6361 tcaagctata acaatgtttc gcaacttaaa agagaagtta atagattgta taatttaaga
     6421 attgatgtct ctcaaggacg gcaacatttt ttaagatatt taatacctca aacttttaga
     6481 gattgggaag tttgtaactt aaaaattgac tccggaatgg gttttaattc caatattggt
     6541 tttagatgtg gaatatgtta tgagtttcca ctttttgatg ttgaactaag aaatcaacta
     6601 caattaagag aacgtccact tattgtaatg gacaaggctc tgcgagcaag atgtgaaaac
     6661 caacaagatt gtgtaaacga aagtgtaaga ctagctaata ttgtaaaatc ttataacggt
     6721 gattttgttc ttttatggca taatagtaat ttggttgtaa atgaatggga ggggtgggat
     6781 agtatttata aagaaattgt agagggaatt taaaatggaa gttattttta caatatttta
     6841 tctttcagga ataataaaaa gttttttgat tttttacaac atcccttttc ctattgattt
     6901 tacttttctt gcctctttct tattattgct tgcacttata atagaattta aaaatgaaaa
     6961 aatttcagtt agttttaaca aaaatatttt attgtctttt gtttttttaa ttctatttta
     7021 tctgtggatg ttgctgagtt taatatggac accgtcagaa agttattctt ataaaaaagt
     7081 aatacttttt attccaaata ttattggttt tggatttcca cttttatgta aaagatttga
     7141 tattgctaag ttttttagaa tagtgtctat tactttaatt tttgtgagca tatggttttt
     7201 aaaaatttat ttggactatt tagatgatgg ttattcaaaa aaaacttatt acgagccaat
     7261 tttaggatta tatctgtctt gtgcgagttt tatgggcttg aatattttgg taatattggg
     7321 agctaaaaaa agaattttta aaaatttatt tgtttcaagt tttgtagtta ctcttactat
     7381 tattttattg tttttacttg gagctagagg tccatttatt tttgtattat tacttttaat
     7441 tccacttttt ataataaaat atatttacag ttattattat aacaaagtat tgctttcaga
     7501 ttttaaaaaa gctttatata gtggattaac ggtattattg ttttttgttt ttttccttat
     7561 ttttgaaaag gaagttaaag ttttacttga tcgttcagta tttagaataa gcttgctgat
     7621 gccttcttct gatagtgaaa ctagcagtgt gcctacaact tctaatacaa ctaaaagcag
     7681 tagtataaat acttctgtcg atacaagaat tgatcaattg aaatttagta tggatttaat
     7741 aaaaaacaat aagtttgaat ctatttatgg atttggtgtt ggtagttttg gaattcttta
     7801 tttacaaaaa gattttagag cttatcctca taatattttt cttgaaattt gggtagaact
     7861 tggtttaatt ggtttgattt tatttttaat atttttatat tttgtttttt caaagaactt
     7921 aaatggtaca aattatatta atttgttggt attggtctat ataatattaa atgttttaaa
     7981 atcaagttct tatgttgata ttagagtttt ctttgctgtt tttgcaatgt atatgctaag
     8041 ttcaaatgat aaatccttga ataaagttgc gaattaaatt atgtttaaaa tgttcccaat
     8101 tttacagaaa taaatactcc tggagaagaa aaagcatcgg tagaatacaa tgttggaagt
     8161 ttacgaaaat aactgtattg atattctacg ccagcagcaa tttttaaaac ttcggaaata
     8221 tcatattcta ttgctaatga aggattaaaa ccccagaaaa cttttttgtc gtggtgttgc
     8281 caatttgtat ctattacttt agtatttcca atactgaatt tcatttctgc aagcccatgt
     8341 aaggcatgtt ttttaaaaaa aggataagca aaccacaaac cgccttgagt acaaccaatt
     8401 ttcatcgaag aaacaataga atcattttta gtaaaagatt ttgccattcc actaaaaaat
     8461 gcacctattc taaagtcttt aacaacaaaa cctccgccac cgcctgtata taaagcgaag
     8521 ttttcatctg aacttctaaa atttaataaa atagccccaa aacctccacc tccaggatta
     8581 tctactggtt cttcaacttg tgaaaatagt ggcagtgtaa agatagataa aacaaataat
     8641 ataaaaattt tcttcatttt taagtaaaaa tttaagtgta aagatataaa aaaaaataat
     8701 tatgttcttt tataaatttg atttgttatt tttgaagcaa aatattttat tatgaaaact
     8761 acactttttg tatatttatt attattgtca ggttttttat tttcccaatc tgttctttcg
     8821 cctgctttgg tttcggaatt aggaaaacat gaaggctatt atattaatgt gaatatttat
     8881 tttcagtcgc attatgatat aaatgaattg gcaagaaatt tagatgctaa aaaggcttct
     8941 tttgatttta gaacaaaatc tgtaaatgat ttgttgaata aaaatgcaga aatttcttta
     9001 agcgaatttg ccgtttgctt agattttatt ttaaatcaag attcaagagc tgtcggaaaa
     9061 ataaaatttt ttcgtgctgc aaatgcttta aatattgaga taaaaccaaa ttttgtttat
     9121 tatttagcag atcaaaaatc aattcgtttt attgatataa attcagcacg ttataaaatt
     9181 attggtaccg aaagagtttt gtcttctgaa cctaagcttg tagggggcac agagcctgga
     9241 ttaaaagcaa taaatgctca caaactttgg gaaaaaggat acacaggacg aaatattttg
     9301 tttttgagta tggacacagg tgttcacgca acacatccag ctatttctga taattttgca
     9361 ggaaattact tgcctttaag tcaatgttgg tatggaattc gtaatcctaa acctgttgat
     9421 aatgcaggtt caagtcatgg aactcatact acaggaacag ttttaggttt ggataaaact
     9481 acaaacgata ctataggtgt agcttttaat gcgaaatgga ttgctagcga cccagttgca
     9541 agtagtgatt ctgatttgtt aacaccagca gattttatgg ctgtttttga ttgggtgtta
     9601 aatcctgatg gcgatgaaac tactagtgat gacgttccac gagtaataaa taattcttgg
     9661 ggctataatt acgatttagc actcaacttt ggtgcttgtg atttacctga agcagaaatt
     9721 ttagttacta tagaaacagc tgggatatgt tctccttttt ctgctggtaa tgaaggacct
     9781 ggagctggaa caacaggttt tccagcaatg ttagcttaca atattgtgaa tccaatgtca
     9841 ataggagctg ttaatggaaa ttctgaaaat ttccctattg ctgacttttc gagtcgagga
     9901 ccttcacttt gtgttgagga agaaggacga ttaaaaatta aaccagaagt tgttgcccct
     9961 ggtgttagtg ttcgttctgc tagtggtgct agctcttatt cttatttaca aggaacttct
    10021 atggcttgtc cacatgtttc tggtgcttta ttgctattag ccgaagcatt cccaatggct
    10081 tcagcttatg aactgaaact tgctttatat gaaaccgcaa tagatcttgg cgaaactgga
    10141 gaagataata tttatggaaa tggaatgatt gatgcactag cagctttcga ttatttgtca
    10201 aatatttata ctccagctcc acctattaca aatgaatatg atttgagttt agagttgaca
    10261 tcttcagaaa aaagttttgt ctgtcaagat gaaaaatttc taaatactca aatgaaaatc
    10321 aaaaatcttg gtgaaaatcc aataaatggc tttaatgtta aagtttatct aaatgaagta
    10381 atagtttgtg attcaactat taatgaaagt atagttgaag gcagtagttt tgagttttta
    10441 tatccaaatt tagaattgtc agcaggagaa aattatattc atggaatagt aaaaccattg
    10501 gaagatttgt ctgaatacga caggtttaat aatggattca atcatagagt gtttttgtta
    10561 agtgaagttg atagttctta ttatcaaaac tttgaaaata tttcaaattt agataattca
    10621 gaatttgtaa taataaatcc cgactataga actacttggt caatgttgaa ttggggcgaa
    10681 aatgacgagc ataaagctat aggtgttaat ttccttgaat atactcccaa aaattggaat
    10741 atagatgatt tatatttgcc agaaattact atgccagcag aaaatgattc tatgttttta
    10801 agttttactt atgcttataa acgcagaaca ttaaatcttt ataaagatag tttaataata
    10861 gaagttagta ctgattgtgg tttgagtttt gataaaattt tgtttgcaga aggaggagaa
    10921 aatcttgcaa ctgtcgaagg aaattcaaac tacaagtttt ataaacctat agaagcctca
    10981 gaatttgata caataaatat tagtttaaat gagtttaaag gcgaaaaact tgtgataaga
    11041 tttagaacaa aaaatgataa tggttcggta ctttatattg atgaaatgaa aataggagta
    11101 ttagataata gctcaattta ttcaaacaag cttgtcgtgc aagagccaga aatttatcca
    11161 aatcctactt cagatattat ttttgtaaaa atctatgaaa gtgaagaatt tgttaatata
    11221 tatgatttga cgggaagatt ggtgttaaat caaaaagttt ataatgaaat aagtaaagta
    11281 aatcttggcg atttaaccga aggagtttat tttatccatt ttattaattc aggtaagact
    11341 aaaaaattaa taaaaaagta aaaatcttaa atattttgtt ttgaaaataa taattttata
    11401 atacatttgt attttacatt aaaaatgatt atttttgaaa atattttaaa atagaaattt
    11461 atgaaaaaag tttttgttat tagtgtttta atgatgatag taggcttagc tttgcaatct
    11521 tgtggtcctg aagatactca tggtccaaaa atttacattt taaatcctga tggtaaaatt
    11581 ttacacggtg aagctaaaga tacaacctta ttattgtgga gtaaatatgt agatccagga
    11641 gtttttgttg aagacaatgc ttcaaaaaca gaagatattg ttgttacact tgatacaaca
    11701 gttttacctc tcaacaaaga aggatatttg agagccacta gaggaacagt tgaggaaatg
    11761 ttgaatttaa cttacacagc tacagactta gcgggtaatg tttcaacaaa tgtgcgtcct
    11821 tttagagttg ctaatgtttc tgaagtgttt gcaggctctt atcttataag ccgaacagct
    11881 atgcatgtta acgatacaaa ttatacttca actattagtg ctgatgcaag agttgcagga
    11941 cgtttaagat ttaataaagt ttatgcacat agatggagtg gtaaaaacac atattttaaa
    12001 gttaatgcag atttatattc ttccgaatta tctccagcat cttttgatga aagatatggc
    12061 tatctttgta aaaaaggtga tccaggagtt tgttattatt ctgggttgac ttatacacca
    12121 gctaaagagg gaatttatga ttattattat ttaaaaattg atgcccaaaa ttatgaagac
    12181 tctttaggta atgttgtgta tattagagga atagaagatg tgaacgatct cccactttct
    12241 aaaattgagt ttttaggaga tacacgtact ataactaaaa ttattttaga attaaacgtt
    12301 accaaaaacg gacaagttga taaagtaaaa gaagtgtata ttccaagata agaaactatt
    12361 gtgagaatta ttatagggct gctttaacaa tgttggagca gcttttttta tatattaatt
    12421 tccccttcaa aaacgtgttc tgcatcgcca cttaaaataa tattttgata acaattagtg
    12481 ttatactcaa aatttacttt taatctacca ccttttgcaa ttaaaactat ttcgttagat
    12541 tttattttgc cagaatggtg caaagctaaa gctgaagcaa cagaacctgt tccgcaagca
    12601 agggtttcgt cttcaactcc tctctcaaaa gtagctatac gaatattgtt gttttttatt
    12661 tcaacaaagt ttacatttgt tctcattggt gaaaatcttg actcgtcagc aatttttctg
    12721 ccttcttgtt ttacgtttat tgaatttaaa tcattaacaa aattaacaaa atgaggcgag
    12781 ccagtatcta aaaaatagcc atcttcaaaa tcttgtattt tatttacatt gctcatcgaa
    12841 agggaaacag agcttctaat tatatttgcc ttatgttttc catctatagc taaaaaattt
    12901 acttccgagc tagaaatttt atttaaatat gcatattttg caatacacct tccgccattt
    12961 ccgcacatgc ttccttcttt tccatctgca ttaaaatatt gcatgtaaaa atcataatca
    13021 tcatttttta gtactaacat taaaccatct gcacctattc caaatcttct gttacataaa
    13081 aattcaattt gtttttgatt aagttcaatc ttattattca tcatgtttat aatgatgaaa
    13141 tcgttgccag ctccttgata ttttgaaaaa tgtaataaca tgatataaat tttatgtaaa
    13201 gtaaataaaa aaatatgttt attttaaaat tatgtgtttt tattttggtt tgcgacagat
    13261 tgccaaatac tccagttttt atttccccat tcatggtata tgtgcaatag tttagggtag
    13321 aagtgggttt taaatccatc ttttgtatat cctaaatttt tgtcccaatc aatatccttg
    13381 tttgcaaact cacaaatcca attaaactct tcgggaatat tagggtgatt ttctagacca
    13441 aatttccaaa cacttaaagc caaagtcccc tgatcgcgag tttttgtata aggatttgaa
    13501 aattctttaa tagtaatttc gtgccaaaag tttaaaaatt cttcagactt atcattaaat
    13561 aaaaatacgc caccattcca atgtcgccaa ttatcaggaa tttttattcc atattctttt
    13621 tgtaaatatt cactaagatg agaacagcta ggtgccttaa attcgtattt ttctcctttt
    13681 ctattttccc ataaattttc ttcaaaatta aatattattc ctgttttgcc ccatattttt
    13741 ttttcataat atggataatc aaaaagaata gcttcgttat cagaattata ccaacattta
    13801 tcagacttga gaaatttaaa ctgtttaagt ttaaattgtt ttattggtaa aatatatctt
    13861 tttatgaagt aagtaatatt ttgaattgaa tactttattg gattgttttg gtattctgca
    13921 aatatttgta aaagttgttg tgattgagct tttatttttg gatttgagaa gtcaattttt
    13981 tcaaaaagtt gggatatttt tattttaaaa gtttcatcat ttttaatttt gtttacaatg
    14041 caagagcaat tcatggcatg aggtgaaaaa tattgtaaag aacaatggtc ttttgcaaaa
    14101 gtaataggtg gtttgtaaaa attaaaaatt tcatctactt tttcattaat cgctacaata
    14161 tctccatcta aataacaata attatgagtt tttggtaaaa atttatttaa gcctgtcttt
    14221 atgaaaatgc tggcttgatg atggtcaaag tcttctggtg tatcaatttg aatgatgttg
    14281 ttatgttcta tttttatttc atttcgtttt aagtcgctta gaacaattat ttcgttttta
    14341 gagtatttac gtaagaattt taatgaaaag tttagctttt ctatatgttc acttccacca
    14401 caaactacaa aaacaaattt attcatacgg tatataaatt tatgctatcc attttttaaa
    14461 ccatttttcc aaaactaata ttttccataa acgccaatgg tctttttgtt taatataatg
    14521 atttatagct gattgattaa taatctcagc ttcaactaat tttgagttat ttaaattttt
    14581 ttcataaaat tcaaaattca tataaaattc atcgggtccc gtaaaacctt gttttttgcg
    14641 atttaaaatt ttctttggta aatctttttg taaaagtttg tataggatta ttttttgctt
    14701 tttcttatca aaatactttt ctgtatctaa gctaagcatt ttttcgcaaa tttctttgtt
    14761 caaaaatggc attctaactt ctaaagaatt tgccatacta gctctatcca tttttgttaa
    14821 taatagctca cccataaatg ccttgatatc aattgcttga aatcgttttg gtttgtctat
    14881 attttcgtta aaatattttc tgtaaaattc ctttgtatca gttggagtat attgatgata
    14941 tttttcggta agtagaaatt ttagttcttt ttcatcaaat tcacccattg ccatagcttt
    15001 tgcataagag tctatatcaa agttatttat gggtaaatcc caattccatt tttttgcttt
    15061 tgttagttcc gcttttgaaa tttcccataa atattcttta tgccaaccat agcctgcaaa
    15121 aaactcatca gcaccttcgc ctgataaaat tattttattg tgttgtgaag ctaatttaca
    15181 agcgaaatat gttggaatta ttgatatatc agcaattggt tcgtcataat aatacattaa
    15241 ttcgtcaaga atatttaaac tttcttggtt tagtagcttt gaaaaatggt tggttttata
    15301 aatttttgat actttttctg caaataaatg ttctgatttt tcccaatttt caaatccaat
    15361 tgaaaaagta ttaatttctt ttttaatttc tgagaaatat ttaactaaaa ttgaagaatc
    15421 aaaacctccg cttaaaaagc ttgcaatatc tgtgtctgaa tctatttgct cttttattga
    15481 attacgaatt aactcttcaa tttctttgat aagcttactt tttctttgtt ttttgatgtc
    15541 aatttttagt tcaaaatatt ttttgtgaga aatattaaaa tttttatcta tttcgatata
    15601 atgtgcaggt ggaattttga aaatgtcttg aaaaattgtg tttggcgaag gaatatacct
    15661 atatataaaa tattcgctaa tggatttttt atttatttct ctttttattt ccgggttttt
    15721 aataattgct tttatttcgg aagcaaaaat taaattttct ttatcgcaga aatagtataa
    15781 aggttttact ccaaatctgt ctttaactaa aaataatttt tgtaatttat tatcaaaaat
    15841 agcaaaagca aacattccgt caattttttc taaaaatttc attccccatt cttgataggc
    15901 atgtattatt agttcgtcat cagagtttga tttaaaaata tgagattttt cctctagtaa
    15961 aattcgtaat tttttataat tatatatttt accattaaaa actgaataaa catttttgtt
    16021 ttcactacat gtaacttgag tatcagactt gctaaaatta ttaaatgcta gtatcctatg
    16081 tcctagtgct aatttttcat cactactaaa atatgagtca tagccatcag gtcctctgtg
    16141 cgttaaggta tcgcgcatgg agttaaataa ttctctatta attttagagt ttttactcca
    16201 ccagcccgtt attccgcaca taattaaaat cgttttttag ttggtcaata tcgaatttgt
    16261 tagcaattct tatgtacttt ttataagcat tcaaggattt tttatattct ttccaagatt
    16321 tttcaagttc ccattttgat tttaacatag atctgtattc tttgcaaatt gtgtcgtaaa
    16381 tttcatattt ataaaatttc cattcagggt cttttaataa attttctaaa attttcacat
    16441 tatttctttt aaatacagtt atgtcgtgaa tttttgaagc accttttggg tggtcattat
    16501 aaacagacca tatttcattg aaatatttta attttgaatt tttaatgatt tctaaatgta
    16561 aagcccaact aagcgataga gcagaagcat tgaaattgtt taaaaatttt gaaactttag
    16621 tgttcctaaa aattaatgat gctgttggga taatatttct tactaataaa tcccaaggca
    16681 taaagtcttc gacatatcta ttaaattgcg agtaggtttt ccagttaatg tgtgttctat
    16741 gattgtagtt tgcatcattt gacttattgt attgttcaat ttttgcgtca tgaaagcatc
    16801 ctgcataatc gggattattt tctaaaaaat caatttgagt ttggatttta ttttcgtaac
    16861 accaatagtc atctccgtct agaaaacaaa aatatttagc cttagctttt tgtgctaaaa
    16921 ttttggcaga atttaatgca ccaatatttt cattattaaa aattaattca attttatttg
    16981 gattttgctg gaaaaagttc tctaatattt ctctagttcc atctgttgaa aaatcatcaa
    17041 caataataat gttgtaatta tatgatgttt tttgacaaag tatagaattg atacacttct
    17101 caataaaatt aaagtgatta tggcataata taccaatgtt gagcatagta tcagactcgt
    17161 ttttcattta attgatttta gaatctatta attttactat atctttaaaa caagttagtt
    17221 tttctaattc ttcatctgtt atttctatat cgtatttttc ttctaaatca attacaatat
    17281 ctacaattct tgcagaattt attttaaggt cactgataat acttgagttg tcgttaaaat
    17341 cattccatac gtttttatca agcgtatatt tttttaaaat atttattaaa tcggttttaa
    17401 tattttccat attttagttt tttcaaagtt aattaaattt ttttaaaatg atacaagaat
    17461 taacatctcc aaacccgaaa cttgcttttg caatataatt caaattaatg tcttttatta
    17521 gtttttgagg gattttattt ttattccaaa tggtttttat gttttcgtta aggtcttcac
    17581 aatttattga aggatgtata aaattatttt gtaattgcaa aatggctgca attgtttcta
    17641 tagctccagc tgcacctatg gtatgtccta ataatgattt taacgagttg atgtacggta
    17701 atttttcgtt taaatttaaa gctgttttcc agttttcaac ttcttggtaa tcaaatttag
    17761 tgcctgttaa atgaccacat attaagtcta tatctttgcc agaaatattt gcattattta
    17821 gtgccgattt aatacaatca attactcttt tagggtttga tgctgtcatg ctacccccat
    17881 ttctttgacc accagaattg tattcataac caactatttc tgcataaatt ttagcatttc
    17941 tatttaaggc atgattaagt tcttcaacaa ttagcattcc agctccagca gatgctacaa
    18001 atccatcggc acttgcactt aaagggcgtg atgcttgagt tggtttgtca ttaaactttc
    18061 tggcaagtaa tctcatagaa tcaaatcccg cccacatata tgggctatag gggtcggcac
    18121 taccagcgat aaataaacta gcttttgatt gtttgatttt ttcactagcc attattattg
    18181 attcagctcc tgtagcacaa gcagatgaat tagatatagt ttggtttgcc aatgcaaata
    18241 tatttgacag tacagcagtt gttccactat gcattagatt ttctattatt tgactaccta
    18301 attttctaat atttttactg tttaccaaag ggtataactt tctagcatat aattcggctc
    18361 caccagcaca agagcctata acagctccat aattttcatt tatgttttca gcgaacttat
    18421 ctggtaattc taattttgcg tcattccatg cttgtagtgc tgctaaaaca gaatatttaa
    18481 ctgttaaatc tgcatctttt aattcgaatt tttcaaatat tttctcatat tcggaattgt
    18541 tgatatctgg aacgccacct atttgcgagg caaaattata atcagctaat tctttaatat
    18601 atttaatgcc tgaaatgcct ttttgcattg atagtttaaa actttcaata tcattaccta
    18661 tagggctaat aactcctaat cctgttataa caacacgttt actcatactt taaacttaat
    18721 tgtgccttgc atacgacaca aagttcttcc aatttattat aaagttttat ttcagatttt
    18781 aatacattat ttctgaaata tattttatct ccaataactt taattttttc tcctcgttca
    18841 actattttat aaaaactact ttccaaatta gaaaataaag gcataaaatt tttgttggat
    18901 aagtgtaaat cgtttaaaaa tattaaatga gataccatgc ctatttgtcc cattgcttct
    18961 attaaaatta cacctggagt tataggtttg tgcaaaaaat gagaattgta aaaatatgct
    19021 gatgatttaa atttataagt tccaactatt ttatctttat caatatattg tatttcatct
    19081 acaaataaaa aatctttacc ataaggtagt ttagaaatta tgcttgaaaa taaataatct
    19141 tgattagttt caatgtgcat atttaaaaag ttttaaaaca caaatataaa aaataaaatt
    19201 atatttgcat aaaacatttg aatattaatt tgttaaataa tggtaaatag caagttccat
    19261 actttatatt ttgaagcaac tagaaattgt aatttttctt gtatatattg ttcaacaggt
    19321 tcgtcaaaga gtaagaaatg cacggatgcc gactatcagg caattgtaga tcatattttt
    19381 gtcccagcaa aaaatttagg aactaaattc attgattttt caggcggaga gtttcttttg
    19441 cgtaatgata gcttaaaatt acttcagacg gctattaata tgggattctc tgttggtatt
    19501 gttagcaatg gtacaacttt aaataatgaa aaattacaac aattaaagga tattgcaggt
    19561 gaggaactca ttatttcgtt aggaattaat tcttttgata gtgataatat agttactaga
    19621 gacaatcatt atactaacac aatcaaactt attgaaaaaa ttagtgggtt tggttttaaa
    19681 attaatatta gtgtaactat tggtgaattt aataaaaaat catttgcaga tacaataaaa
    19741 aaaatatctg atatgtcttt ggcatttaat cgaattccat tttctattag aaattgtaat
    19801 gctttggatt tgatgattac tccagacagt atgaaaaact attttcatcc agttttgaga
    19861 aattacttta atgggcagat tagttatgtc ccattttttt tacctccaga ctcttataat
    19921 acaattacta aaagtaatat ttataatgat gaattgccct taaatccaag tataggttgt
    19981 tgggtaggtt catattattc tatcaatccc gaaggcgagg tttctccatg tccattattt
    20041 ggtgatcaga ttagtggagg aaatgttttt aaagaaaacc ttaaagatat tttatttgag
    20101 tcagaacttt ttaaaaaaat tctgaataga aaaaatttta aaggtaaatg tggaaattgt
    20161 aaatatactt taacttgtgg tggctgtaga gtaatggcat attttttaaa tggagatcca
    20221 tatgctgaag acccaatttg ttttattgat aaattaagta atgaagaaat agaagcaatt
    20281 gaaaaagaaa caatcaaaag ttttaaaaat tataacagaa tgataaaaat tcgcaatcta
    20341 ttaataaata aagactcatg aatacaaata aagaatttat cctaataata ggtgggagtg
    20401 gtagtatcgg tttagcttgc gcaaaaatgt tagctaaaaa aaaatataat ttggttttaa
    20461 ttcatagaga ttctcgctca atgcaaaaat taagtcaagt agagtttgat aaaattagaa
    20521 attctggtat agaattgtat aatatcaatg taaatgccaa taccgatgaa gcttttataa
    20581 aattaaaaga acttttaagt gaaaataata taaaatctat aaaagcattt attcatgctt
    20641 atgctgatgc aaatattggt agtattttag agtcagaaaa agaacttagt gcagatgatt
    20701 ttattcatag ttttaattct atggcattaa gttttgtaac ttggtcaaaa tggttgaaac
    20761 aaaataatta tttacaaaat ttttcaaaaa taataggttt taccagcgaa ggtagtttta
    20821 gagtgttgaa caactatgtt gcggtaggaa tggcaaaatc agcattggaa tcggcatgta
    20881 aatatttagc tatagagttt gcaccatatg aaattaatgt gaatttaatc aattcaggaa
    20941 ttgttttttc aaaatctgtt gaattaatgg aaaagcaaaa accatttatt gaaaaagcta
    21001 taaaacgaaa tccatttaaa cgattgacag agccttctga tattgcaaaa gttgtttctt
    21061 ttttgatttc tgatgactcg aactggatta cagggcaaaa aataacagtt gatggaggag
    21121 aacagttgtt gagcatataa attaattgta actatcttaa taagaaaata atgaataaaa
    21181 acgtaatatt actattttac cctttagtcg ataaagataa gcagattcct aatttacctt
    21241 tttcggtttt gaatttggaa agaatgctaa gagatttagg tttggaaatt ataattattg
    21301 atgaaagagt aatagtggat tacaagcaaa taattttaga aaataaagat aggcttctta
    21361 tggctggcgt tagtgctatg attggatatc agatgatttc aggaaaagag ttttctttat
    21421 tttttaaaca acattcagat gctcctttag tgtggggagg ttggtttgca aatgttttgc
    21481 cagaacttgc attaaaagaa aattttatag attttattat tcatggacaa ggcgaaatcc
    21541 cttttaaaaa ttttgttgaa acctttatca gaggtgaaga ttttaaaaat atcagaggac
    21601 taggatacaa aatagataat aaaatagtga taaatcctat agagaatatt gttgatgaaa
    21661 aaagttttcc aaatattaac tttaatctcg ttgatgtaaa taaaattgca aatattaatg
    21721 agcccgataa tacaggtgca agtagtctta attatattgc aactattggc tgcccatata
    21781 gttgtgcttt ttgctgttta gccagtatct ggggacaaaa aactttcaat aaagatgtgt
    21841 ctttaa
//
LOCUS       DAOPNX010000039        21222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_3083_length_21222_cov_7.071526, whole genome shotgun sequence.
ACCESSION   DAOPNX010000039 DAOPNX010000000
VERSION     DAOPNX010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21222
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3083_length_21222_cov_7.071526"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..551)
                     /locus_tag="PKN53_05815"
     CDS             complement(36..551)
                     /locus_tag="PKN53_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88500.1"
                     /translation="MLLEILKITIPSLIVFLTAYFVLKAVLKKEHENRKIEVVLQNKK
                     IITPLRLQAYERIVLFLERISPNSIIIRLQTPNMTVGQLHKEMLTTIRTEFEHNLSQQ
                     LYLSISAWEEVRNAKEKTIKLLNSCLEDLDLNDNALIYSQKIFETLIEIEKSPTQEAI
                     NFVKEEVAQLY"
     gene            645..1838
                     /locus_tag="PKN53_05820"
     CDS             645..1838
                     /locus_tag="PKN53_05820"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOD88501.1"
                     /translation="MEKVSDRLNKMPVSATLAMAQKSRDLQAQGIDVINLSIGEPDFD
                     TPQHIKDAAKKAIDDNFTHYPPVPGYESLRKAIVEKFKRENNLEFKTDQIVVSTGAKH
                     SIINVFLTILNPGDEVIVPAPYWVSYPAMVGLAEGEPVFVKCNIDSDFKMTAAQLEAA
                     ITPKTRALIFSSPSNPTGNLYSKEELKSLAKVIEKHPNIIVVSDEIYEHINFVGKHES
                     IAQFPEIKDRVVVVNGVSKGYAMTGWRIGYIGAPTWIAKAVTKLQGQFTSGACTIAQK
                     AAEAALNAGVDEPHKMTAIFNKRKDAVLKFMKDIPGLKTNIPEGAFYVFPEVSYFFGK
                     TYDGKTINTSNDLALYLLEKAHVATVAGDAFGSPECLRLSYATSEELMLEALKRIKKA
                     LAELK"
     gene            1947..>2420
                     /locus_tag="PKN53_05825"
     CDS             1947..>2420
                     /locus_tag="PKN53_05825"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOD88502.1"
                     /translation="MRKILFFISVFILANYANSQITTNYSLSDALISDNTRCFAEDNN
                     GNIWIGSSAGISVFDGSDFSYFTTEDGLGGNVIYDFLVHSNGNVYAATSGGLSKYNGV
                     SWMNYDAGSGLPSNSIWCVDEDIDGKIWVGTSSNGAAYYDGVNWHVLSENQGLISN"
     assembly_gap    2421..2520
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2521..6198
                     /locus_tag="PKN53_05830"
     CDS             <2521..6198
                     /locus_tag="PKN53_05830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD88503.1"
                     /translation="KIFGSTFTTYDNTHGLSNKIVNKILITKEDNNSQIWVGSPFNGL
                     TVFDMNQDFVIYRMNKNIVGNNISTIFTDENNTIWNGTNDGLNKVLDRNWRTFKVSDG
                     LVNNMITAIHKDQNQNVWIGTQNGLSKISGQTIVNFNTPNLSNSYINDITSDNTGKVY
                     VATRSKVNIIENDEVVNIIDVDQGLANDTIKKIHFENGRIWFLSDAAIQYFDGSNYID
                     ATALCAGNYSQAGAVCSNTSNIQYFGDDYSLRIYDVNNTTYNCIPHPYSGVSKIVSMT
                     KIGNKIYCVFENGELQYYDGTWTNVALTFSATWVEHYLDENYILLGSENQGLFKLCLN
                     CSSDIVTTNNPPCLEGNNGSITITSPSGSQYSINNGNTWQASNTFSNLKRGYKHILIK
                     NSLNKIIADKEIYLESYNGHIGSSNLTITQIDCFGNNSGEISLDYDPSASHVWENYNT
                     TLLVRENLNPGTYSVTITDGASCTKVLTNSIVQPEQLVTSHTYNNITCYGLANGSIDL
                     TIEGGTQPYSILWSNGDTNEDINNLMPTTYIYTVTDANNCTSSNSQQITQPNQLEVSA
                     DIVNNLCHGDTNGSIDITISGGTTPYDITWSNTDYVNAQNDIVNAPADTYTLNIVDQN
                     NCSIEETYTITEPEGILLSLDNLEHVKCYGENTGKIIIDVSGGYGALSFEWKKDSETE
                     VFSTEQNIENLFAGTYHLSITDANNCQRTETYTVSQSPALSASINVTPITCAGSNDGQ
                     MSASATGGSGTYSAYTWYNSEDQIIGVNQTINNLGAGDYYVVVRDSYYCYATAYASLT
                     QATPHVYETSHTDMTCNGLNNGTITITIDGGSGAGFNFNWQSGVSSTTNTASNLGPGT
                     YFVTVTDPTNCVEILEETIIEPPMQSIGCFDDYGYVCYGNSLTLNPGNFVSYIWSTGE
                     ITQSIDVENEGTYFVEVVDNQGCHLGDTIQLVVNAVYNNEEINLASVDNQGSVTLMWN
                     KTPNHGTEYFKIYKDIGNGYDFLTNVYFNQPAIYTDTDVDTENHYYKYKISVVDSCGA
                     ESQLSEHHRTCLLSGVGDNNGASWLNWGAYEGFFVVYYFIERGSTPNNLEVVDSVLYN
                     QFNWAEMNPNPDGSYYRIKVRRMDGCNPGDGNYYSEAYSNIVFCNNNVGFVNNAIVSP
                     QVYPNPFKDEINIEFYQNIPGEVSYSITNMLGQTVESSDKIYFNQGNNTLKFNTELQN
                     GVYILKLNFREESHSFRIIKN"
     gene            6200..6922
                     /gene="truA"
                     /locus_tag="PKN53_05835"
     CDS             6200..6922
                     /gene="truA"
                     /locus_tag="PKN53_05835"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOD88504.1"
                     /translation="MRYKIELSYKGTNFHGWQRQPIATTVQQVLEDAINLIFRDKIEL
                     VGCGRTDSGVHAKYYVAHFDSEKNYTEQAVKRLNKFLSSDIAISSISKVNDDFHARFS
                     AKERSYIYHITEIKNPFNTDFAWHITKKLNVELMNQAIQKLYNYTDFTSFSKLHSDVK
                     TNNCKIINAEVEKINSDIFIKITADRFLRDMVRAITGTLVDVGKGKISILDFCKIIEE
                     KNRCKAGQSVPAHGLSLVDVKY"
     gene            6935..7627
                     /gene="cmk"
                     /locus_tag="PKN53_05840"
     CDS             6935..7627
                     /gene="cmk"
                     /locus_tag="PKN53_05840"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOD88505.1"
                     /translation="MNKTIKIAVDGHSSCGKSTLAKDLAKYFDFLYIDTGAMYRAVTL
                     FAIRKGVFKNDKENYELLLKNLSEINIEFKQTSKDKTEIYLNGENVEYEIRNLEVSSK
                     VSPIATVPEVRTLLVDLQRKMSENKSVVMDGRDIGTVVFPNAEVKLFVTADVETRAKR
                     RFDELKQKNIDVDYESIFKNITERDFIDQNREVSPLKMAEDAILIDNSKLTIKQQLDL
                     AVKIIKEKIYEN"
     gene            7617..8474
                     /locus_tag="PKN53_05845"
     CDS             7617..8474
                     /locus_tag="PKN53_05845"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007751648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOD88506.1"
                     /translation="MKIEIDKHAGFCFGVTRAIKEAEENSSLGIACLGQIVHNAKEEK
                     RLNKLGMKTVTKEDFPSLAGKTIMIRAHGEPPETYTKAENLNIKIIDATCPVVLKLQQ
                     QIKEKYNLDKNSIIVIYGKKGHAEVNGLVGQTNGEAIVIGSIEEAKNLDVSKNIYLFS
                     QTTSDYFKYQEILNFLEKKSKVFHGNTKQFKAFQTICPSVKNRIPKLKDFCLNHDIII
                     FVSDSNSSNGKMLFGICKETNKNSFFVTSTNDIDKSWFANVKTVGISGATSTPFWLME
                     NIKTKISSL"
     gene            8519..9265
                     /locus_tag="PKN53_05850"
     CDS             8519..9265
                     /locus_tag="PKN53_05850"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HOD88507.1"
                     /translation="MKRTIAILSIVFLLASCDVLTGVLNETGSGSTTPKQLTQAEVAE
                     GLKEALSVGIGNVVNVVSATNGFYNNAQIKIHFPEEAIKVKEFCIDAGLKNQVIQFEE
                     KLNRAAEDASKGAKEVFITSIKQMTITDAISILKGNDHAATDYLKRTTSKQLYDKFYP
                     VVTASTSKIMLAKYWTPLVTKYNAAMTITGGQQVDTDLNAYVTNKALDGLFLMVAKEE
                     KNIRIDPIARVTDILTKVFGSDLNPYNKNK"
     gene            9312..10577
                     /locus_tag="PKN53_05855"
     CDS             9312..10577
                     /locus_tag="PKN53_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88508.1"
                     /translation="MLIAVMLTASIPPRTPFVVLRVDGTDYKAGSEIKVRQGERIIVE
                     AVLMGGKRDYCSNPNTYANVGRNTVVLSQGENGMSFQINNYQFTGDWKLTEEKANFSS
                     GPEVKIKSLGSGNMQRKVELEFTRGYYSKVFLKVNSTTKWHYTRKTPGGKTEDNETNT
                     GSATFYFLVETTDSEWYSSNNIKATGQEDLSVRNNLDRIQKYYDAIEKAMLEKNWSSA
                     ETQWGNLKTSLGELKTNIDRAKQKNENYECEITLYNLPSTLSMKHVEDLKIMAQSWKD
                     CWEICSENVLTIKDDLLSNKTVFTNNVMKSIFKNYISWSGQLPTSASSILTLYDPKGI
                     FTALDLPNNIIEWYTDAQNDASILKNQVNNMKLLNQLHDFYKAKMDNSINERRALLNI
                     LQDLKPAIELHNKMGQYISGVSSVKLVKK"
     gene            complement(10804..11160)
                     /locus_tag="PKN53_05860"
     CDS             complement(10804..11160)
                     /locus_tag="PKN53_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88509.1"
                     /translation="MKKFYTLLVFLFFATIVFSQVLKDPRIESMSGGVSYSSGNYLLF
                     IDPRGYIQKTDMEIRDYRNLPDNLSATQRTNTEQQREIDELKRTISNLERKLGEMERD
                     LYNQKRQIEDLQRKVR"
     gene            complement(11203..12252)
                     /locus_tag="PKN53_05865"
     CDS             complement(11203..12252)
                     /locus_tag="PKN53_05865"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOD88510.1"
                     /translation="MKKIVYVLFVLFIVVYFSNCEKNYDLCHSKSQWLKDYFAELKSG
                     NYPNIYAISWWHEDFDNTYFKINSSKRSLKTYKELVSDDIFISSCSFVNKKLVPVSGK
                     IYHSSFPCFSGTEDEVTSQRIEDFESLVEKEIAWAYFSNNWMDSLVFPKNSIDIILEK
                     GKTPFIRLMFRSVFEQNQEDPKYKMKDILNGRYDAAIIAWANEAKNCSTNLLVEFGTE
                     VNGVWFSWNGYYYGGGTCDEYGDPNYPDGPEIFRDSYRHIIDLCNQQGCDNITWFFHF
                     DVNDAPEEWWNNPIYYYPGDDYIDWIGVSTYGPFQRGDDYIEPKEIINKAYKKMQNVS
                     TLKPYAILEFGVTEL"
     gene            complement(12395..13054)
                     /locus_tag="PKN53_05870"
     CDS             complement(12395..13054)
                     /locus_tag="PKN53_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HOD88511.1"
                     /translation="MKRVIIICEGETEREFCQKVLAPHLAQHDIFIQAPLIKKSIGGI
                     VRWSVLKKEIETYLLESNVIVTTLIDYYGLLKKYNFPKWDEGERIVDKNARMDFLEKA
                     MKDDVLDNVRHRYIPYLQLHEFEGLLFNNIQLFYNQVPESELVGKAELKKTFDDYDNP
                     EMINNNKETSPSHRLKRIIKGYNKVLYGHYFAEAIGIEQIRAKSPRFNSWIDKIIKIE
                     Q"
     gene            complement(13051..14118)
                     /locus_tag="PKN53_05875"
     CDS             complement(13051..14118)
                     /locus_tag="PKN53_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009231621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD88512.1"
                     /translation="MDYIEISGYKSIKNTRIDLAPINILIGANGSGKSNFISFFDFLN
                     RLYNRKLNEYVALKGGDNKILHKGKKNTDTISFKMEFDNGQSGYSAKIQSGTDGFVFL
                     NENLIYKGDSQSISKPGFEAQIKISDNHNAPCVIKYLNGFRKYHFHDTSLNSPFSKHS
                     NIENDIYYFYEDGMNLAAFMYHIYETNKIVYNRIVKNIQSIAPYFSDFYFAPSKENII
                     RLQWTDKYSDVVFGANDLSDGTLRFIALSVLFLQPNLPDTIIIDEPELGLHPSAIAKL
                     AGMIKSVSDRGCQVIVATQSTDLISHFLPEDIITVDQIDGQTVFARLSSSNLKQWLED
                     YTIDDLWKRNIITGGQLNYKL"
     assembly_gap    14189..14288
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14335..14553)
                     /locus_tag="PKN53_05880"
     CDS             complement(14335..14553)
                     /locus_tag="PKN53_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88513.1"
                     /translation="MDIHAKKYQLIEWITNISDIKLIDKLMKIAEKTDWWNEISEAER
                     ASIERGLKDIEENRVFDHSEVRKNHFIN"
     gene            complement(14783..16867)
                     /locus_tag="PKN53_05885"
     CDS             complement(14783..16867)
                     /locus_tag="PKN53_05885"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOD88514.1"
                     /translation="MKKGLIFSFFILLSFVGFSQILIKPQNPEVFAYSGDNQTQTLGK
                     TTANPIRVLVLDENKNPIQNYEISFSIISYPQFSKGFQLTDKKVKTDENGIASTYLQI
                     GDQEGEYTIMASSANDLDVDCLVYTVNARDKSWIFFLIAGLLGGISIFLYGMNILGKG
                     MVSATGNKMRNLTSKFIKNRFSGFIAGFVLALLVQSNTAASVLTIGFVESGLVSFVQS
                     LSMLLGAAVGTTITTQLIALNITDYALFIIAIGFGTVMFSRNNKFKYIGRAILGLGLL
                     FMGMYVISETMAPLKNYTKFVEILIGLENPFIGIAVGFIFTMALNSTIAFIGIIITVS
                     TQGFLSIEAAIPLILGSNMGNTLIPIIASLKAGREAQRAALANILFKFSAVLIFVWWI
                     PELANLAKSFSPSRLSHVSDYEYFAETVPHQIANVHTFFNIGMAIVMLPLINVYAYLI
                     KKILPDKKEKSPFELNYIDMAVNVSPALTLSLAKKEALRMGDKIKELVSLCLLPFIER
                     NENVLKIWQALENEADFLQKNINKYLVTVSSQSVDEQQLNEAFQIMYIVKELEMIGDI
                     VNTNLRHQAVKWLASDYSFSEEGKAELETIHLKALKQISRSMEVFAEFDKERAEHVRN
                     KYKQYAELAESLEKSHYERLFTQNQQTLSSSEVHLELLGLLNSVNRHATNIARILVNW
                     QKNKVKKSNKMN"
     gene            16974..17960
                     /locus_tag="PKN53_05890"
                     /pseudo
     CDS             16974..17960
                     /locus_tag="PKN53_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013815486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            17946..18923
                     /locus_tag="PKN53_05895"
     CDS             17946..18923
                     /locus_tag="PKN53_05895"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88515.1"
                     /translation="MWGLLSYLTIWIISLVFLGGYDKPAKLKNVYKGIIVGSLFILVV
                     YSLLPANLRFSRAIILLSTAWALILIPLVRLLLHYTGLNCFKINLPGKKKVAIVGSET
                     EAKDLVKLIKNTNPKFVISSFVSPSKDENSEFFSGNLEQLNEIVKIDKIDEIIFCAKN
                     LQAQQIIRTMLKLDNPRLDYKIASPDGFSVIGSNSTNTTGELYNIDINSIIKPENLRN
                     KRLLDFCISFLMILFYPILVLFMKQPKQKLKNLFSILLGNKTFVSYYEGEGVDTSMLP
                     KIKKGIYTPLENFKKAELNPELAQRINLMYAKDYRVTQDMNIIVKSWRK"
     gene            18911..19453
                     /locus_tag="PKN53_05900"
     CDS             18911..19453
                     /locus_tag="PKN53_05900"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOD88516.1"
                     /translation="MAKINSQLSNDNILLRKPEPEDLDFLYSLENDTNIWWVSDTRSP
                     FSRWQIKQHIENSVYDIYTNKELRLIITDKHNGKALGVIDLFQYEPAHQRAGVGIVIL
                     QEYRNQKIAEQSLEIIIEYCFNIINLNQIWCCIDAKNLISIKLFKKFGFVKTGELKEW
                     KKIGENKFEDVYLYQLKAMH"
     gene            complement(19823..20293)
                     /locus_tag="PKN53_05905"
     CDS             complement(19823..20293)
                     /locus_tag="PKN53_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOD88517.1"
                     /translation="MTTKFPVLSYKTYDFRLKEEDGKLNIFDELRKKWLVCTPEEWVR
                     QNLIRFLIEEFGFPANLIAIEKSLSLAGRNYRFDALVYDRQFSPLMIIECKSPYVKLE
                     QKVFDQIWTYNYEINAPFYLVTNGIAFIMGECRSGKTPSFFKEVKGFENLIKEI"
     gene            complement(20293..20754)
                     /locus_tag="PKN53_05910"
     CDS             complement(20293..20754)
                     /locus_tag="PKN53_05910"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HOD88518.1"
                     /translation="MFRFFAIIFFLNISYVSFSQEVIDFPHWLAGTWEIESPTGVSYE
                     EWKLSDNSELIGRTYRLFGNDTLVFDNMRIYNLGSATVYEMFVNCEYDRELMKFSLQK
                     PDDNLWKFENIKSNSPKNINYYRIEENVVYVWTEHASDETACIDFLMKKKK"
     gene            20908..>21222
                     /locus_tag="PKN53_05915"
     CDS             20908..>21222
                     /locus_tag="PKN53_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88519.1"
                     /translation="MKRLFYLALFVIATMLFSCKDSSQTLVGKWNIVDIHQEAQDSLT
                     DLTIPTVIFGMQTTGVSYVEFTKDNEYHVFDKENQIIETQKYSIDKNKLSIQGKDGKV
                     DVV"
BASE COUNT         7664 a         3224 c         3126 g         7008 t
ORIGIN      
        1 aaaaactaaa aactcaacac taaaaactct tttaattaat aaagttgcgc cacttcttct
       61 tttacaaaat ttattgcttc ttgagttgga gatttttcga tttcgataag agtttcaaaa
      121 attttctgac tataaatcaa agcattatca ttcaaatcta aatcttctaa gcaagaattt
      181 aaaagtttta ttgttttttc ttttgcattt ctaacttctt cccaagcact aatcgacaaa
      241 tataattgtt gagaaagatt atgttcaaac tctgttctaa ttgttgttag catttcttta
      301 tgcaattgtc caacagtcat atttggagtt tgcaacctaa taattattga atttggcgaa
      361 attctttcca aaaacagaac tatacgctca taggcttgca gtcgcagagg tgtaataatc
      421 tttttgttct gcaaaactac ctctattttt ctgttttcat gttctttttt caacacggct
      481 ttcaacacaa aatatgctgt taaaaacact atcaaagacg gaattgtaat ttttaaaatt
      541 tctaataaca tgtcaaatac tttttagata aatatgtttt acaatattat aaaaataaaa
      601 aataagttct tatattagca aataaatttt ataaacaatt tacaatggaa aaagtatctg
      661 atagattaaa caaaatgcca gtatcagcca cgttggctat ggcacagaaa agccgtgatt
      721 tgcaagctca aggtattgat gttattaatt tgagtattgg cgagcccgat ttcgacactc
      781 ctcaacacat aaaagatgct gcaaaaaaag ctattgacga caattttact cattatcctc
      841 cagttccagg atatgaatct ttgcgtaaag caattgttga aaagtttaaa cgcgaaaata
      901 atttagagtt taaaaccgac caaatagtag tttctactgg agcaaaacat tcaataataa
      961 atgttttctt gactatttta aatcctggag acgaagttat tgttcctgct ccttactggg
     1021 ttagctatcc agctatggta ggcttagcag aaggtgaacc tgtatttgtg aaatgcaata
     1081 tagattcgga ttttaaaatg actgccgcac aattagaagc tgcaataact ccaaaaacaa
     1141 gagctttaat ttttagttcg ccttcaaatc ctacaggaaa tttatattca aaagaagaac
     1201 ttaaaagttt agctaaagta attgaaaaac acccaaatat tattgttgtt tctgacgaaa
     1261 tttacgaaca tataaatttt gttggtaaac acgaaagtat tgctcaattc cctgaaatta
     1321 aagatagagt tgtggttgtt aacggtgttt ctaaaggata tgcaatgaca ggctggcgta
     1381 ttggttatat tggcgctcct acttggatag caaaagcagt tacaaaactt caaggacaat
     1441 ttacttctgg agcttgtact attgcacaaa aagctgccga agctgctcta aacgctggag
     1501 tagatgagcc tcataaaatg actgcaattt ttaacaaacg taaagatgca gttcttaaat
     1561 ttatgaaaga tattccagga ctaaaaacaa atattccaga aggagctttt tatgttttcc
     1621 cagaagtttc ttatttcttt ggaaaaactt atgacggaaa aacaataaat acttcaaacg
     1681 atttagcatt atatctttta gaaaaagctc acgttgcaac tgtggcagga gatgcttttg
     1741 gctcgccaga atgtctaaga ctgtcttatg caacaagcga agaactaatg ttagaagcac
     1801 taaaaagaat caaaaaagcc ttggctgaat tgaaataaga aaaaaactaa cgataagtgg
     1861 ttaataaata aaaatattaa ccactttttt tgccttgttg ttataaaaaa tgtctatatt
     1921 tgctaaattg ttataaattt tacatcatga gaaaaatttt attttttatt tcagttttta
     1981 ttttagcaaa ctacgctaac tcccaaatta ctacaaacta ttccttatca gacgctctta
     2041 taagtgataa tacaagatgc tttgctgaag ataataatgg caatatttgg attggcagta
     2101 gtgccggcat ttctgttttt gacggaagcg atttctcgta tttcactacc gaagacggac
     2161 ttggcggaaa tgttatttat gattttttag ttcatagtaa tggaaatgtt tatgcagcaa
     2221 cttctggcgg attgtcaaaa tataatggag tttcgtggat gaattatgat gctggtagcg
     2281 gattgccttc aaactctatt tggtgtgtgg acgaagatat agatggaaaa atttgggtgg
     2341 gaacttcaag caatggggct gcttattatg acggtgttaa ctggcatgtt ttgtctgaaa
     2401 atcaaggatt aatttctaat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 aaaatttttg gaagcacttt tactacttat gacaacactc atggactttc aaataaaatt
     2581 gtaaacaaaa tattaattac aaaagaagac aacaatagtc aaatttgggt tggctcgcca
     2641 ttcaacggac ttacagtttt tgacatgaat caagattttg taatttatag aatgaataaa
     2701 aatattgttg gcaataatat tagcacaatt tttactgacg aaaacaatac aatttggaat
     2761 ggaacaaatg atggtttaaa taaagttttg gatagaaatt ggagaacttt taaagttagc
     2821 gatgggcttg taaacaatat gattacagca attcacaaag accaaaatca aaatgtgtgg
     2881 ataggaactc aaaatggttt gagcaaaatt tctggacaaa caattgtgaa ttttaacaca
     2941 ccaaaccttt ctaattcata tattaatgat attacttctg acaacacagg aaaagtttac
     3001 gtagcaacaa gaagtaaagt aaatataatc gaaaacgatg aagttgttaa tattatagat
     3061 gttgatcaag gtttagcaaa cgatactatt aaaaaaattc atttcgaaaa tggtagaatt
     3121 tggtttttga gcgatgctgc tatacaatat tttgatggga gtaattatat tgatgcaaca
     3181 gcattatgtg caggaaacta ttcacaagca ggagctgttt gttctaatac aagcaacatt
     3241 caatattttg gagacgatta ttctttaaga atttatgacg ttaataatac aacatataat
     3301 tgcattcctc atccatattc tggtgtttca aaaattgttt ctatgacaaa aattggaaac
     3361 aaaatttatt gcgtttttga aaatggagaa ctacaatatt atgatggcac ttggacaaat
     3421 gtagctttaa ctttttctgc tacttgggtt gaacattatc ttgatgaaaa ttatatttta
     3481 ttaggctctg aaaatcaagg gctttttaaa ttatgtttaa attgcagttc tgatattgta
     3541 acaacaaaca atccaccttg tcttgaaggg aataatggaa gtattacaat tacttctcct
     3601 tctggaagtc aatattcaat taataatgga aatacttggc aagcatcaaa tactttctct
     3661 aatttaaaac gtgggtacaa gcatatttta ataaaaaata gcttaaataa aattattgct
     3721 gataaagaaa tttatttaga atcttataat ggacatatcg gctcttcaaa cctaactatc
     3781 acacaaatag attgttttgg aaataattcg ggagaaatta gtttagatta cgacccatca
     3841 gcaagtcatg tttgggaaaa ttataacact actcttttag tacgagaaaa tcttaaccca
     3901 ggaacttatt ctgtaacaat tacagacgga gcttcttgca caaaagtatt aaccaactca
     3961 atagttcaac cagaacaact tgttacaagt catacttaca acaacatcac ttgttatgga
     4021 ttagcaaatg gaagcattga cttaactatt gaaggcggta ctcagcccta ctctatttta
     4081 tggtcaaatg gcgacacaaa cgaagacata aacaacttaa tgccaaccac ttacatttac
     4141 acagttactg atgcaaataa ttgtacaagt tcaaattctc agcaaattac tcaaccaaac
     4201 caattagaag tttctgcaga cattgtaaat aatctttgtc atggcgacac aaatggaagt
     4261 attgacatta caattagtgg tggaactacg ccttatgata ttacttggtc aaatactgat
     4321 tatgtaaatg ctcaaaatga tattgtaaat gcacctgctg acacatatac tttaaatatt
     4381 gttgaccaaa acaactgtag catagaagaa acttatacaa ttactgaacc agaaggaata
     4441 ctattaagtt tagataatct tgaacatgta aaatgctatg gagaaaatac tggaaaaata
     4501 attattgatg tttctggtgg ttatggagct ttgtcttttg aatggaaaaa agatagcgaa
     4561 actgaagttt tctcaacaga acaaaatatt gaaaatttat ttgctggaac atatcattta
     4621 agcataacag atgctaacaa ctgtcaaaga acagaaactt atacagttag tcaatctcca
     4681 gcattaagtg caagcattaa cgtaacgcct attacttgtg caggaagtaa tgatgggcaa
     4741 atgtctgctt ctgcaacagg cgggtctgga acctacagtg cttatacttg gtacaattct
     4801 gaagatcaga ttataggtgt taaccaaact attaacaatc tgggagctgg agattattat
     4861 gttgttgtta gagattcata ttattgttac gccacagctt atgctagcct aacacaagct
     4921 acaccacatg tttatgaaac ttcgcatacc gacatgactt gcaatggatt aaataatgga
     4981 acaattacta taactattga tggaggttca ggagcaggtt ttaactttaa ctggcaaagc
     5041 ggagtttcaa gtacaacaaa tactgcatca aatttaggac caggaacata tttcgtaaca
     5101 gttaccgacc caacaaattg tgttgaaatt ttagaagaaa ctattataga accacctatg
     5161 caaagcattg gttgttttga tgactacgga tatgtttgct acggcaattc attaacttta
     5221 aatcctggaa attttgtttc ttatatttgg agtacaggag aaataacaca aagcatagat
     5281 gttgaaaacg aaggcactta ttttgttgaa gttgttgata atcaaggctg tcatcttgga
     5341 gatacaattc aactagttgt taatgctgtt tataataatg aagaaattaa cttagcaagt
     5401 gttgataatc agggaagtgt aacattaatg tggaataaaa ctccaaacca tggcacagaa
     5461 tatttcaaaa tttacaaaga cattggaaac ggctatgatt ttcttacaaa tgtatatttt
     5521 aaccaaccag caatttatac cgacacagat gtggacacag aaaatcatta ttacaaatac
     5581 aaaatttccg ttgttgactc ttgtggagca gaaagccaat tatctgaaca ccacagaact
     5641 tgtcttttaa gcggtgttgg cgacaataat ggggcaagtt ggttaaattg gggagcttac
     5701 gaaggatttt ttgtagttta ttattttata gaaagaggtt ctactccaaa caacttagaa
     5761 gttgttgact ctgttttgta taatcaattt aattgggcag aaatgaatcc aaatcctgat
     5821 ggttcatatt atagaattaa agtaagaaga atggacggtt gcaatcctgg cgatggaaat
     5881 tattattcag aagcatattc taatattgta ttttgtaata ataatgtagg atttgtaaac
     5941 aatgccattg ttagtccaca agtttatcca aacccattta aagacgaaat aaatatagag
     6001 ttttatcaaa atattcctgg agaagtttct tattcaataa caaatatgtt aggacaaact
     6061 gttgaaagct ctgacaaaat ttacttcaat caaggaaata atacccttaa atttaatact
     6121 gaattacaaa atggagtata tattttaaaa ttgaatttta gagaagaaag ccatagtttt
     6181 agaataatta aaaattaaag tgcgttacaa aatagaactt tcatataagg gaacaaattt
     6241 tcatggctgg caacgtcaac ctattgctac aacagttcag caagttttag aagacgctat
     6301 aaatttaatt tttcgcgata aaatagaact tgtaggttgt ggcagaactg atagtggtgt
     6361 acatgcaaaa tattatgtag ctcattttga ttcagaaaaa aattatacag aacaagctgt
     6421 caaaagatta aataaatttt tgtcttcaga catcgccata agttcaatca gcaaagtaaa
     6481 tgatgatttt catgctcgat tttcagctaa agaaaggtcg tatatttatc atatcacaga
     6541 aataaaaaat ccttttaaca cagactttgc ttggcatata acaaaaaaac ttaatgtcga
     6601 attaatgaat caagccatac aaaaacttta taattatacc gattttacaa gttttagcaa
     6661 attgcactct gatgtaaaaa ccaataattg caaaataata aatgcagaag ttgaaaaaat
     6721 aaattctgat atttttataa aaatcactgc tgatagattt ttaagagaca tggtaagagc
     6781 aattactgga acattagttg atgtgggaaa agggaaaatt tcaattctag acttttgcaa
     6841 aattattgaa gaaaaaaacc gatgcaaagc tgggcaatct gttcctgcac acggactttc
     6901 tttagttgat gtgaaatatt aaaaaatttt atttatgaat aaaacaataa aaatagcagt
     6961 tgatggtcat tcttcttgcg gaaaaagtac cttagccaaa gatttagcaa aatatttcga
     7021 ttttttatat atcgacactg gagcaatgta cagagctgtt actttatttg caataagaaa
     7081 aggtgttttt aaaaatgata aagaaaatta tgaattattg cttaaaaatt tatccgaaat
     7141 aaatatagaa tttaaacaaa cttcaaaaga taaaactgaa atttatctaa atggcgaaaa
     7201 tgtagaatac gaaattcgca atcttgaagt ttcttcaaaa gttagtccaa tagcaactgt
     7261 gccagaagta agaacactat tggttgattt acaaagaaaa atgtctgaaa acaaatctgt
     7321 agttatggac ggcagagata ttggaactgt agtttttcca aatgcagaag taaaactttt
     7381 tgtaactgct gatgtggaaa ctcgtgccaa acgcagattt gatgaattaa aacaaaaaaa
     7441 catagatgtt gattatgaat ccatttttaa aaatattact gaacgcgatt ttattgacca
     7501 aaacagagaa gtttcgccct taaaaatggc tgaagatgca atattaatag acaattccaa
     7561 gttaaccatt aaacaacaat tagacttagc tgttaaaata atcaaagaaa aaatctatga
     7621 aaattgagat tgataaacat gctggatttt gctttggagt aactagagct attaaagaag
     7681 ctgaagaaaa ttcaagtctt ggaattgctt gcctcggaca aattgtgcat aacgcaaaag
     7741 aagaaaaaag actaaataag ctaggaatga agactgttac aaaagaagat tttccttcac
     7801 tagcaggcaa aaccataatg attcgcgctc acggcgaacc gccagaaaca tatacaaaag
     7861 cagaaaactt aaatattaaa attattgatg ctacttgtcc tgtagtttta aaattacaac
     7921 aacaaatcaa agaaaaatat aatttagata aaaacagtat tatagtaatt tatggcaaaa
     7981 aaggacatgc tgaagtaaat ggtttagtag gacaaacaaa tggcgaagct attgttattg
     8041 gctcaataga agaagctaaa aatttagatg tttctaaaaa catttatctt ttttctcaaa
     8101 ccacatctga ttattttaaa tatcaagaaa ttttaaattt tttagaaaaa aaatctaaag
     8161 tatttcacgg caatactaag caatttaaag cattccaaac tatttgccca agtgtaaaaa
     8221 accgaattcc taaacttaaa gatttttgct taaaccacga tattataatt tttgtaagcg
     8281 attcaaatag ctctaatggc aaaatgcttt ttggtatatg taaagaaaca aacaaaaata
     8341 gtttttttgt aacctcaact aatgacattg ataaatcttg gtttgcaaac gtaaaaactg
     8401 taggaattag cggtgcaaca tctacaccat tttggttaat ggaaaatatt aaaaccaaga
     8461 ttagttcgct ttaataaaaa ttaattctta aatttgcatt attcaaaatt ttaaaaatat
     8521 gaaaagaaca atagcaatat tatcaatagt gtttttactt gcaagttgcg atgtgttaac
     8581 tggagtttta aacgaaacag gttctggtag cactacacca aaacaactca cacaagcaga
     8641 agtagcagaa gggcttaaag aagctttaag tgttggaata ggaaatgttg taaacgtggt
     8701 ttcagcaacc aatggctttt acaataacgc tcaaataaaa attcatttcc cagaagaagc
     8761 aataaaagta aaagagtttt gcatagatgc tggattaaaa aatcaagtta ttcaatttga
     8821 agaaaagtta aaccgtgctg ccgaagatgc ttctaaagga gcaaaagaag tttttataac
     8881 atcaatcaaa caaatgacta ttacagatgc tatatcaatt ttaaaaggaa acgaccacgc
     8941 agcaactgat tatttgaaac gcactacttc aaaacaactt tacgacaaat tttatcctgt
     9001 tgttactgca tcaacaagta aaataatgtt agcaaaatac tggacacctt tagttactaa
     9061 atacaatgca gcaatgacta ttactggtgg tcaacaagtt gataccgact taaatgctta
     9121 tgtaacaaac aaggctttgg acggattatt tttaatggta gctaaagaag aaaaaaacat
     9181 aagaatcgac cctattgcaa gagttacaga tattttaacc aaagtttttg gaagcgattt
     9241 gaatccttat aataaaaata aataaccaaa attaatttaa aataaaaaaa ctaaatttat
     9301 tatcagcatt tttgttaatt gcagtaatgt taactgcaag tattccacct agaacacctt
     9361 ttgtcgtttt gcgagttgat ggcacagact ataaagcagg ttctgaaatt aaagttcgcc
     9421 aaggtgaaag aattattgta gaagcagtac ttatgggcgg aaaacgtgat tattgttcaa
     9481 accccaacac ttacgcaaat gtaggaagaa acacagtagt cttatcacaa ggcgaaaacg
     9541 gaatgtcgtt tcaaataaat aattaccaat tcacaggcga ttggaaatta acagaagaaa
     9601 aagcaaattt tagctcaggt ccagaagtga aaattaaatc attaggctct ggcaatatgc
     9661 aaagaaaagt cgagcttgaa tttacaagag gctattattc aaaagtattt ttaaaagtaa
     9721 attctactac aaaatggcat tatacaagaa aaacacctgg tggcaaaaca gaagataatg
     9781 aaaccaatac aggctcagct actttttatt ttttagtaga aacaactgac agcgaatggt
     9841 attcttcaaa caatatcaaa gcaacaggtc aagaagattt atcagttaga aacaacttag
     9901 acagaataca aaaatactat gatgctattg aaaaggcaat gttagaaaaa aattggagct
     9961 ctgccgaaac acaatggggt aacttaaaaa ccagtttagg agaacttaaa accaatattg
    10021 atagagctaa acaaaaaaat gaaaattacg agtgtgaaat tacgctttat aatttgccgt
    10081 caactttatc aatgaaacat gttgaagact taaaaataat ggcacagtcg tggaaagatt
    10141 gttgggaaat atgctctgaa aatgttttga caattaaaga tgacttgcta agcaataaaa
    10201 cagtgtttac aaataatgtt atgaagtcta tatttaaaaa ctacatttct tggagtggac
    10261 aattaccaac ttctgcctca agcattttaa cactttacga ccctaaaggt atttttacag
    10321 cactcgattt accaaacaat attatagaat ggtacacaga tgctcaaaac gatgcatcaa
    10381 tacttaaaaa tcaagtaaat aatatgaaac ttctaaatca attacatgat ttttataaag
    10441 caaaaatgga taactcaata aacgaaagaa gagctttact aaatatttta caagacttaa
    10501 aacctgcgat agaacttcat aataaaatgg gacagtatat ttctggagtt agtagtgtaa
    10561 aacttgtgaa gaagtaaata tagattagat atattcaaaa actatttaaa aaaccaacta
    10621 atttattgtt aattagttgg tttttttatg caataaaatg cttagcataa gacaaaaaac
    10681 atcaatgtta tgacataaaa accaaaataa atatcaacgc attgatataa taagtcgcaa
    10741 aatgtatcaa tgtgttgaca ttaattttaa aaaaagtgtc aattgaatga cattaatttg
    10801 gtttcacctc acttttcttt gtaaatcttc aatttgtctt ttctgattat acaaatctct
    10861 ttccatttcg ccgagttttc tttcaaggtt tgaaatagtt cgttttaatt catctatttc
    10921 tctttgttgt tctgtgtttg tcctttgtgt tgcgcttaaa ttatctggta aatttctgta
    10981 atctcttatt tccatatctg ttttttgaat atatcctctc gggtcaataa ataacagata
    11041 atttccagaa gaataagata ctcctccact catgctttca atacgaggat ctttcaaaac
    11101 ttgagaaaag acaattgtcg caaaaaacaa gaaaactaat aatgtgtaaa attttttcat
    11161 gggaatttaa tttttattta gaactatatt tatttctcaa ccctacaact cagttactcc
    11221 aaattctaat attgcatagg gtttaagagt tgatacattt tgcatttttt tatatgcttt
    11281 atttattatt tctttaggtt caatataatc atctcctcgc tgaaaaggac catatgtgct
    11341 tacacctatc cagtcaatat aatcatcgcc agggtaataa taaattggat tattccacca
    11401 ttcttcgggg gcatcgttta catcaaaatg aaaaaaccat gtaatattat cacaaccctg
    11461 ctgattacat aagtcgataa tgtgcctgta agaatctcta aatatttctg gaccatctgg
    11521 atagtttgga tcgccatatt catcacatgt gccgcctcca taataatagc cattccaaga
    11581 aaaccaaaca ccatttactt ctgttccaaa ctcaactaat aaatttgtac tacaattttt
    11641 tgcttcgttt gcccaagcaa taattgcagc atcatatctc ccatttaaaa tatctttcat
    11701 tttatattta ggatcttctt gattttgctc aaaaacactt ctaaacataa gacgaataaa
    11761 tggcgttttt cctttttcta aaataatgtc tattgaattt ttaggaaata ctaaactatc
    11821 catccaatta tttgaaaaat atgcccaagc aatttctttt tctaccaagc tttcaaaatc
    11881 ttcaattcgc tgacttgtaa cctcatcttc tgttccagaa aaacaaggaa atgaggaatg
    11941 ataaattttc cccgatactg gaacaagttt tttatttaca aatgaacaac tagaaataaa
    12001 aatatcatca cttaccaatt ctttataagt ttttaacgaa cgcttagaag aattaatttt
    12061 aaaataagtg ttgtcaaagt cttcatgcca ccacgaaatt gcataaatat ttggataatt
    12121 gccagatttt aattctgcaa aatagtcttt caaccattgt gactttgaat gacacaaatc
    12181 atagtttttc tcacaattag aaaaataaac tacaataaat aaaacaaata aaacatatac
    12241 aatcttcttc ataactagtt tttgttcatt gttacttcaa taaaataaat aatatggtgc
    12301 attttgtctc atattttttc aatatatacc aaaaatacaa aatttgggac atatgaataa
    12361 tttttgttaa gtaagatttt gttgagattt agcattattg ctcaattttt attatcttgt
    12421 ctatccaact attaaatcgt ggtgattttg ctcttatttg ttcaatacct attgcttctg
    12481 caaaataatg cccgtataag actttattat aaccttttat tattcgtttt aatctatggc
    12541 ttggagaggt ttctttattg ttgtttatca tttctggatt gtcatagtcg tcaaaagttt
    12601 tctttaattc agcttttcca accaattctg attcagggac ttgattgtaa aaaagttgaa
    12661 tattgttaaa aagtaaaccc tcaaattcgt gcagttgcaa gtatggtata tatctatgtc
    12721 taacgttgtc taaaacatca tctttcattg ctttttctaa aaaatccatt cttgcatttt
    12781 tatcaacaat tctttctccc tcgtcccatt ttggaaaatt atattttttt aacaatccat
    12841 aataatcaat aagagttgta acaatcacat tagattctaa caaataagtt tctatttctt
    12901 ttttcagtac agaccaacga acaattcctc ctatagattt tttaatcaaa ggtgcttgaa
    12961 taaatatatc atgttgtgcc aaatgaggtg ctaaaacttt ttgacaaaat tctcgttccg
    13021 tttctccttc acaaataatt attactcgtt tcatagctta taattaagtt gtccgcctgt
    13081 aataatgttt cgtttccaca aatcgtcaat agtatagtct tcaagccatt gttttagatt
    13141 gctactactt aatcgtgcaa atactgtttg tccatctatt tggtcaactg taataatatc
    13201 ttcgggtaaa aaatggctaa ttaaatctgt tgattgcgtt gcaacaatta cttggcaacc
    13261 tctatctgat acagatttta tcattcctgc taatttggct attgcagaag ggtgtagtcc
    13321 cagttcgggc tcgtcaatta taatagtatc tggtaaattt ggttgtaaaa ataaaacaga
    13381 caatgcaata aaacgcaaag ttccgtcaga aaggtcgttt gctccaaaaa ctacatcact
    13441 atatttatct gtccattgta atcttatgat attttctttg cttggagcaa aataaaagtc
    13501 cgaaaaataa ggagctatag attgaatatt tttaacaatt ctgttgtaca caattttatt
    13561 agtttcataa atatggtaca taaatgcagc caagttcata ccatcttcat aaaaataata
    13621 gatgtcattt tcgatattgc tgtgttttga aaatggagaa tttaaacttg tgtcatggaa
    13681 atggtacttt ctaaacccat tcaaatattt aattacacat ggagcattat ggttatccga
    13741 aatttttatt tgtgcttcaa aacctggctt actaatactt tgagagtcgc ctttataaat
    13801 taaattttca ttcaaaaaaa caaaaccatc tgttcctgat tgtatttttg cagaataacc
    13861 actttgtcca ttatcaaact ccattttgaa tgaaatagtg tctgtattct tttttccttt
    13921 gtgcaatatt ttgttgtccc cgccttttaa agcgacgtat tcattaagtt ttctattata
    13981 tagcctgttt aggaaatcaa aaaatgatat aaaattactt tttccactac catttgcacc
    14041 aatcaatatg tttatagggg caagatcaat tcttgtattt tttattgatt tataaccact
    14101 tatttcaata taatccattt ctcttttttg tttattgata caaattaatt ttccaaaatc
    14161 aatacaaaga taaagttttt taatttatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14281 nnnnnnnnat tgatacaaat taattttcca aaatcaatac aaagataaag ttttttaatt
    14341 tataaagtgg tttttcctaa cctcagaatg gtcaaaaact ctattttctt ctatatcttt
    14401 taatcctctt tctattgatg ctctttctgc ctcgctgatt tcgttccacc aatcagtttt
    14461 ttcagcaatt ttcattaatt tatctattag tttaatatcg ctaatattag taatccattc
    14521 aatcaactga tatttttttg catgtatgtc catgttattt ctttttaaaa tttataaaac
    14581 atcccaatct ccacaattta aaattctact aattacaaag ataaggattt ttaatttatt
    14641 aagtggtttt tataaaaatt tgtgtttaat cttgaatttt ctttttgact ttgctacctc
    14701 tcgcggattt gtaattgtag gcattattct agcactcgga ttacaaatcc gagcgagcgg
    14761 gcaaaaacaa aaacattaca ctttaattca ttttattgct tttctttact ttatttttct
    14821 gccaattcac taatattctg gcaatatttg tagcatgacg attaacagaa tttaacaaac
    14881 ctaacaattc taaatggact tctgaactac ttaaagtttg ttgattttga gtgaacaatc
    14941 tctcataatg acttttttca agactttcgg ctaattctgc atattgttta tatttatttc
    15001 gcacatgttc agcacgttct ttgtcaaact cagcaaacac ttccattgac ctgcttatct
    15061 gttttaaggc ttttaaatga attgtttcaa gttctgcttt accttcttcc gaaaaactat
    15121 aatcagaagc caaccacttt acagcctgat gtctaagatt tgtatttaca atatcaccta
    15181 tcatttccaa ctctttgaca atatacataa tctgaaaggc ttcgttaagt tgctgctcat
    15241 caacactttg actgctgaca gttactaaat atttattaat gtttttttgt aaaaaatcgg
    15301 cttcgttttc taaagcttgc catattttca aaacattctc atttctttca ataaaaggaa
    15361 gtaaacaaag cgaaacaagc tctttaattt tatctcccat tcttaaagct tctttttttg
    15421 ctaaactcaa agtaagcgct ggcgaaacat ttactgccat atctatataa ttgagttcaa
    15481 atggcgattt ttctttttta tcaggcaaaa ttttcttaat caaataagca taaacattta
    15541 taagaggcaa cattactatc gccattccaa tattaaaaaa tgtgtgtaca tttgcaattt
    15601 gatgtggcac tgtttcggca aagtattcat aatcactcac atgcgacaat cggcttggcg
    15661 aaaatgattt agccaaattt gcaagttcgg gaatccacca tacaaaaatt aaaactgctg
    15721 aaaacttaaa taaaatattt gcaagagctg cacgttgagc ttctcgtcct gctttaagac
    15781 ttgcaattat aggaatcaaa gtatttccca tatttgaacc aagaattaaa ggaatggcag
    15841 cttctatact taaaaaaccc tgtgtcgaaa cagtaataat aataccaata aaagctattg
    15901 ttgaatttaa tgccattgta aaaataaagc ctacagcaat tcctataaaa ggattttcta
    15961 agccaattaa aatttcaaca aattttgtat agtttttcaa aggagccatt gtttctgaaa
    16021 ttacatacat tcccataaac aacaagccta aacctaaaat agctcgtcca atatatttga
    16081 atttattatt acgcgaaaac ataacagttc caaatccaat cgcgattata aaaagggcat
    16141 aatcagtaat gtttaatgca ataagttgag tagtaatagt ggttccaaca gccgcaccca
    16201 aaagcataga caaactttgt acaaaactaa ctaaaccact ttcaacaaaa ccaatagtaa
    16261 gaacacttgc agcggtatta ctttgtacta acaaagctag cacaaaacct gcaataaaac
    16321 ccgaaaagcg attttttata aatttactgg ttaagttccg catcttgttt cctgttgccg
    16381 aaaccattcc ttttccaaga atattcattc cataaaggaa aattgaaatt ccgcctaata
    16441 gtcctgctat taaaaagaat atccacgatt tatctcttgc atttacagta tatacaaggc
    16501 aatcaacatc taaatcatta gcagaacttg ccataatggt atattcgcct tcttggtcgc
    16561 caatttgcaa atatgttgaa gcaataccgt tttcgtctgt cttaactttt ttatcagtta
    16621 actgaaaacc tttagaaaat tgtggatatg aaattatcga aaaactaatt tcgtaatttt
    16681 gaataggatt tttattttcg tctaaaacta aaactcgaat tggattagct gtagtttttc
    16741 ctaaggtttg tgtctgatta tcaccagaat aggcaaaaac ctcggggttt tgaggtttta
    16801 ttagaatttg tgaaaagcct acaaaactta acagtataaa aaaactaaat attaatcctt
    16861 ttttcaaaac caaaaacttt aataattaca aaaatataaa taatttttat ttctatacat
    16921 tttataaaat tgcaatcata cagttttttt ataactttgt cgtttgattt tttatgaaac
    16981 tttcggttgt aatagtaaat tataatgtga aatacttctt agagcagtgt ttaaattctg
    17041 tatttgaatc ggctaaacat tgcgaaaccg aagtttttgt tgtggataat aattctgttg
    17101 acggctcatg tgcaatggta aaatctaaat ttccacaggt aaaattaatc gaaaacaaaa
    17161 caaattacgg cttttcatac gctaataatc aggcaattaa acaatctaaa ggagaatatg
    17221 ttttattatt aaatcctgat actgttattg aagaaaaaac tttaaaaaca gtttgcgatt
    17281 ttatggattc tcaccctgat gctggtggtt taggcgtaaa aatgattgat ggaaaaggtc
    17341 gttttttacc cgaatcaaaa agaggcttgc caacacctaa agttgctttc tataaaattt
    17401 ttggattatc taaattattt aaaaaatctg caaaatttgg tcaatatcac ctttcttatt
    17461 tagataaaga taaaactcac gaagttgatg ttctttctgg tgcttttatg ttgctaagaa
    17521 aagaagcctt agataaagtt ggacttttag acgaagcatt ttttatgtat ggcgaagata
    17581 ttgatttatc ttacagaata actttaggcg gatataaaaa ctattacctt cctgaaacta
    17641 caattattca ctacaaaggc gaaagtacaa aaaaaggtag cgtaaattat gtgttggttt
    17701 tttataacgc aatgataatt tttgcaaaaa aacacttttc caaaaaaaat gcaggctttt
    17761 ttatagcttt aataaaaata gcaatttacc ttagagctgc tcttgccatt ttccatagat
    17821 ttttgcaaaa tattattacg cctctaatag atttaataat aattttagca ggatttttaa
    17881 tcatcactcc tatttggtca gaatttcagt ttggagtaag agacgcttac cctcaaaata
    17941 tttttatgtg gggattactc tcttatttaa ctatttggat aatttcttta gtttttttag
    18001 gaggctacga caaacctgcc aaattaaaaa atgtttataa aggaataata gttggctcac
    18061 tctttatttt agtggtttac tcacttttgc cagccaattt gcgtttttcg cgagcaataa
    18121 ttttacttag cacagcctgg gcacttattt taattcctct tgttaggctt ttgctacatt
    18181 acacaggatt aaattgtttt aaaataaatt tacccggaaa aaagaaagtt gctattgttg
    18241 gtagcgaaac cgaagccaaa gatttggtga aattaattaa aaatacaaat cctaaatttg
    18301 taatttcttc ttttgtaagt ccaagtaaag atgaaaacag cgaatttttt tctggaaatt
    18361 tagaacaact caacgagatt gtaaaaattg acaaaattga tgaaataata ttctgtgcta
    18421 agaacttgca ggctcaacaa attattagga ctatgcttaa actcgataat ccacgtttag
    18481 attataaaat tgcaagtcct gatggttttt cagtaattgg aagcaattcg acaaacacca
    18541 ctggcgaact ttacaatatt gatataaata gcattattaa acccgaaaat ttaagaaaca
    18601 aaagattatt ggatttttgt atttcatttt taatgatttt gttttatcca atacttgttt
    18661 tatttatgaa acaacctaaa caaaaattga aaaatctatt ttctatattg cttggcaaca
    18721 aaacttttgt ttcatattac gaaggcgaag gtgttgatac cagtatgctt ccaaaaataa
    18781 aaaaaggaat atatactcct ttagaaaatt ttaaaaaggc agaattaaat ccggaattag
    18841 cacaaagaat aaatttgatg tacgcaaaag attaccgcgt aacacaagat atgaatatta
    18901 ttgtaaaatc atggcgaaaa taaattcaca acttagcaat gataatattt tacttcgtaa
    18961 gcccgaaccc gaagatttag attttcttta ctcattagaa aacgatacaa atatttggtg
    19021 ggtaagtgat actcgctcgc ctttttcgcg ttggcaaata aagcaacata ttgaaaattc
    19081 tgtctatgat atttacacaa ataaagaatt aagactaatt atcaccgaca aacacaacgg
    19141 aaaagcatta ggtgttatcg acctttttca atacgaaccc gcacatcaaa gagctggagt
    19201 tggaattgta attttacaag aatatcgtaa tcaaaaaatt gcagaacaaa gcttggaaat
    19261 tattattgag tattgcttca acatcattaa tttaaaccag atatggtgct gcattgatgc
    19321 aaaaaactta attagcatta aactttttaa aaaatttgga tttgtaaaaa ccggcgaact
    19381 taaagaatgg aaaaaaattg gggaaaataa gtttgaagat gtgtatctat atcagttaaa
    19441 agcgatgcac tgaacgtagt tgaagtgtta aaagttaaaa gcgatgcact gaacgtagtt
    19501 gaagtgttaa aagtggctga cgcgtctaaa aataattgaa taattgataa ttgataattg
    19561 aataattaag aatttagaat tattttagtt aaatggtttg tgtgttctac ggacaaaaat
    19621 ctctaacaaa aaaataacgt gtcgaaaaaa ctgaaaatct tcgacatgaa aaaataacgt
    19681 gtcgaaaaaa atggaaatct tcgacatgaa acaacaattc agttgaaagt taaaagcgat
    19741 gcactgaacg tagttgaagt gttaaaagtt gaaagagttt tagtgttgag tttttagttt
    19801 ttgaacttta cgaactttta actcaaattt ctttaataag attttcaaaa cctttaactt
    19861 ccttaaaaaa acttggagtt tttcccgaac gacattcgcc cataataaaa gcaatgccgt
    19921 tagtaactaa ataaaaaggt gcattaattt cgtaattata tgtccaaatt tggtcaaaaa
    19981 ctttttgttc taattttaca taaggagatt tgcactcaat aatcatcagc ggagaaaact
    20041 gtctatcata aactaaagca tcaaaacgat aattgcgtcc tgctaaagac aatgattttt
    20101 caatggctat taaatttgca ggaaaaccaa actcttcaat taaaaaacga attaaatttt
    20161 gcctaaccca ttcttctgga gtacaaacca accatttttt cctaagctca tcaaaaatat
    20221 tgagttttcc gtcttcttct tttaaacgaa aatcataagt cttatacgat agcacgggaa
    20281 attttgtagt cattacttct tctttttcat taaaaaatct atacaagcag tttcgtctga
    20341 tgcgtgttct gtccacacat aaacaacatt ttcttcaatt ctatagtaat taatattttt
    20401 gggactatta gattttatat tttcaaattt ccataaatta tcatctggtt tttgaagaga
    20461 aaatttcatc aattctctat catactcaca atttacaaac atttcataca cagtagcaga
    20521 gcctaagttg taaattctca tattatcaaa aacaagagta tcatttccaa acaatctata
    20581 tgttcttcct attaattctg aattgtcaga caatttccat tcttcatacg aaacacctgt
    20641 aggagattca atttcccaag ttcctgcaag ccaatgtgga aaatctatta cttcttgcga
    20701 aaaactcaca taagaaatat taagaaaaaa aattatcgca aaaaatctaa acataaaaac
    20761 ataagtttat aatggttttt caaaattact aaattattaa aaattacttc acaaaattct
    20821 ataaaaaact aactttttat ctttattaaa aataaatttt gtatctttac actaaattaa
    20881 ataattttta actaaaacca tttaattatg aaacgattat tttatcttgc attatttgta
    20941 attgcaacaa tgcttttttc ttgtaaagac tcatcacaaa cacttgttgg taaatggaat
    21001 attgttgata ttcatcaaga agcacaagac agtttaactg acttaactat tcccacagtt
    21061 atttttggta tgcaaactac aggagttagt tatgtagaat ttacaaaaga caatgaatat
    21121 catgtgtttg acaaagaaaa tcaaataatt gaaactcaaa aatattctat agataaaaat
    21181 aaattgagta ttcaaggaaa agatggaaaa gttgatgttg tg
//
LOCUS       DAOPNX010000040        21179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_3094_length_21179_cov_6.755255, whole genome shotgun sequence.
ACCESSION   DAOPNX010000040 DAOPNX010000000
VERSION     DAOPNX010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21179
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3094_length_21179_cov_6.755255"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..1845)
                     /locus_tag="PKN53_05920"
     CDS             complement(250..1845)
                     /locus_tag="PKN53_05920"
                     /inference="COORDINATES: protein motif:HMM:NF019291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HOD88520.1"
                     /translation="MFVTKIKIIGNEPEKEYAQTVSLEHRKKFAQFFTPYVVAEFMTE
                     WILGNKQLKTVLDPAFGLGIFARAIREIDKNCLINGFEIDKNILYKAKEIFEKDLNTN
                     IVFKDYIFNDWGNRYDGIICNPPYFKFHDYDNKAMLKEIEEKLGLKLNGFTNLYTIFL
                     LKSTYQLNKNGRAAYIVPSEFLNSDYGKLVKFYLLKNKFLRYVMVFDFEENVFDDALT
                     TASILLFANDDEKSGVEFINIKTIDELNSLQKKIRSYPIRKSENLVSFSELKPEIKWR
                     AYYQKQNAKHFKNLVPFTNYGKVVRGIATGANDYFTFSKSKAEKFNISEKNLLPCITK
                     SVDVCGSFFTKDKFEELKKQDKSVYLFKVNDLQEKSVINYIAKGEMEEINKRYLTASR
                     NPWYSLEKRSPSPIWVSVFNRNGLRFIRNEANISNLTTFHCIYLNMFSKSKVDLFFAY
                     LLTNVSKEIFNDNRREYGNGLNKFEPNDINKALMVNLDLINKEDEKKILNLYKNYRKS
                     ELSGNANTSLLEAINDIFVSIFT"
     gene            complement(1845..2945)
                     /locus_tag="PKN53_05925"
     CDS             complement(1845..2945)
                     /locus_tag="PKN53_05925"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AccI family restriction endonuclease"
                     /protein_id="HOD88521.1"
                     /translation="MNYYEQIRNLIKNVPLSLVDFSQPRELARIPTQASSNFITNKEQ
                     GDWAESLVAKAINDVSKNFVAIKYGKSDDLIAGDEGFDEFYQEFQNELDTIGKRPDLL
                     IFKKTDFNDQLGYDISQIPHSTIADYVKKAIAGIEVRSSAFLIDRYEKAMQIRTENFS
                     QFALQTRDKILAEYLDLLEQPNRCKYIPILKSISVETLSVADFKVPGWSSSERLIELN
                     NHFKELKRAIKEIQKRDFLSITPKVEDIKVVYKWIETFNVPHIYFQVFFDKVYGISFE
                     QILTIISDPEKEGVVFSVEADTKNQNKTIIKINSKSGIQIAYKVDEPEHKSVRKEMNR
                     GRLLFYVTFKGGAAYLDVNNLCEILGIKQNDF"
     gene            complement(3100..4467)
                     /locus_tag="PKN53_05930"
     CDS             complement(3100..4467)
                     /locus_tag="PKN53_05930"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOD88522.1"
                     /translation="MKNILISILTLIIFNATIVNAQTNVYSLDSCKALAIENNIDISS
                     QKLEVELAKYTKKQAFTNYFPKVSATSGGFIANKHLIDVDINDIDIELYLENQHYNDI
                     LTTLYNTYGVYFQNTDLTARALKDGVFAGITAVQPVFAGGRIVTGNKLANLGITASEY
                     QIELYLKDILLKTESAFWQIISLEEKLTTINVAKSMLDTIYKDVTGAAEAGLITNNNV
                     LTVKLKIHEINSNLLRLNNGIKLAKMALCQQIGIEYNDSIKFVDNIATIENPEQIKKV
                     HAEVLPNRIEYKLLNLSVEAENLQKRMKLGEVLPQAAVGVGYMYNNFLEKHNANLIGF
                     FTVSIPISDWWQNKYSMKYHKTKCEIAAQKRDDMQEKLMLQMQLAWNELVEAYSQVQL
                     LEKTIDEAEANLKLVNDNYQAGMVSVSELLQSQTLLQQSKDQYTDAKINYKIKYLMYK
                     QMVGD"
     gene            complement(4464..7568)
                     /locus_tag="PKN53_05935"
     CDS             complement(4464..7568)
                     /locus_tag="PKN53_05935"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOD88523.1"
                     /translation="MKLGKNIFSNTMRYHSLITLAVIVFVIFGIYGLFKMNKQEFPVF
                     TIRQGVIVGVYPGATPNEVLEQLTKPLEEYLFTFQEINKQETYSYSRDGLVFVFVKLN
                     NSVKNQTETWSKIRHGLKDFKAMLPTGVLALIVNDDFGNTTSLLITIESADKTYRELD
                     TYVSKLTDQLRTIDAIGNIKVHGKQNEEIHIHVEPEKIAAYGISRETLLLNLFTQGAQ
                     MASGAITNEDISLPIHITSPYRSEEELSEQIILSEPGGKVVRLKDVATVKRSYPLDAS
                     YIRSNGNKTLVVSIEMRPGNNVVEFGREVDEILNNFKNTIPQSIKIERITDQPEIVGS
                     SVRSFLTDLGVAILIVIVVMLMLFPLRSALVAATSIPVSIAITLAFMYLFGIELNTVT
                     LAVLIVVLGMVVDNSIVVIDGYIDNLNRGHSRWYSSVESARTYAPSLTMATIAICALF
                     FPILGIFTGEMSDFVKSFPPTMTIALMASLLLALFVIPYYQFLFIKKPSETDKVGLIA
                     RLQNKFFHHLQIGYEKLLTSCFKKPRTTIFVALMSIGVAVFLLMITPVQMMPKADRDT
                     FAVEIFLPEGTALEQTAMLSDSLRKIIEKEEDVVSVTEFIGTSSPRFHMTYAPKLPGS
                     NYAQFIVKTKSDEATISLLKKLEPMYSNYFPNAYVRFKQMDFQAVSNPIEIRISGDDV
                     SKLLEQAQKVETILHDFDDDLTWIHNDFNGTMPFVKIEILPEEAMRLGITKSYLSTSL
                     AINFHGLPLTTIWEEDYPVAVKLIRNLDNNWADFEDLKNEIVPTVIPGTWVPLRQVAK
                     IAPDWQISQLVRRNGVYCITVSADMKFDKKESVVIKKIKKRVKNEILPEMPSNLKLEY
                     GGLSEVNKNTVPEVITCIVAALVVIFTFLLMTFKHVSISVLSLSANALCMFGVFVGLY
                     LFKQDLSLTSVLGIVNLIGIMVRNVIIMYEYAEEQRTVHKLSAYQAGLEAGKRRMRPI
                     FLTSMTTAVGVIPMILSKSTLWMPMGIVIFVGTIFSLIAVITVLPVMYWLVFNKKSPV
                     KASK"
     gene            complement(7565..8593)
                     /locus_tag="PKN53_05940"
     CDS             complement(7565..8593)
                     /locus_tag="PKN53_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD88524.1"
                     /translation="MTNYKFKLIFFFIIILFISSCNNDKSNSNNGEIAVNVMEASIDS
                     FDKLHDYMGVIEENSSILLSFEVGGNIESIFVTEGQSIAKGQVVSEINKEASQNAYEL
                     AKSTLDRAEDGYKRAEQVYNNGSLAEVKWIEIQTQLSQARASEKIARRNLDNCVLRSP
                     QSGVVGELMVSPGMNILPYQPAFKLMDITIVKASVNVPEKEISKISVGQNAEVRISAV
                     SEEAFKGKVIEKGVSADKLSHSYVVKIGIDNTDKRLMPGMTCRANITTGKKYKGFKVP
                     VNAVQLSNDGSKFVWVVEDGKSTRKIVEVGDLIDNHVIITSGIKENEKIIVNGFTKVS
                     EGCKVKIQ"
     gene            complement(8599..9063)
                     /locus_tag="PKN53_05945"
     CDS             complement(8599..9063)
                     /locus_tag="PKN53_05945"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HOD88525.1"
                     /translation="MMKIVGKILGIFLLVFIFSNNLYSQVKADDIVGVYLTYDKLTKQ
                     NCKIEIFKLHDNTYSGKVIWLEKPLDKDGKPYCDVLNPDPSKRHVRCDNMTILYGFTF
                     KKGEWVDGKVYNPTNGKTYTAKMNFETKTKLKLRGYVGHPMFGATEYWTKIK"
     gene            complement(9073..9579)
                     /locus_tag="PKN53_05950"
     CDS             complement(9073..9579)
                     /locus_tag="PKN53_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88526.1"
                     /translation="MMKKNKLPLLLFCFFTILFYSCDNKPVKNFLGDFTYKTSGSITV
                     EGDTSDVALKITNKLGQMDIIDIGEENRVMVIKTEITGNITKTYATISADDENKIIFD
                     TIPISEIFISGLDTVSSETNIYSAPVGKRYEDRIVIEDFYFGYVDTYKAKGPIKQSDV
                     ITISEKNK"
     gene            9793..12258
                     /locus_tag="PKN53_05955"
     CDS             9793..12258
                     /locus_tag="PKN53_05955"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOD88527.1"
                     /translation="MNYLRLILSFIFLYFLTTNLYSQNKFAGRVLDAKTNEALAFVNI
                     TYNSNNYGTTTDLDGYFTINSADKIEFLKLSYLGYTTKLITKDEIQNKKYIEISLEQD
                     AFYLSEVTILPGINPAHRIIDLAIENSNKNNPEKIRSFSYVSYNKMIFTVDMSNIQAD
                     TTKTNETKTDTTDKKTLREFFDEQHIFITESVTERRFRYPDQNKETVLAHKMSGLKNP
                     AFTLLTTQFQSFSFYKNFISLLDKKYLSPISRGSTSKYFFQIEDTMYNENADTIFIIS
                     YRPSKGKNFEGLQGVLHINSNGYALQNVSAKPYEPTGSFNINIQQKYELIDSIQWFPV
                     QLNTDLVFNFFQVGENEAVISSGNSDTKSSSYAPLVGIGKSYISDISLNPDYKRREFN
                     QLSVEVEKNAHKKDKDFWDYYRYEKLSDKDIKTYQVIDSIGREINLDKTMELMEVIAT
                     GYIPIKFVNLNPASILWYNKYEGYRLGLALATNHKLVKFLSLGGHFGYGFKDKAWKYG
                     AYIDWFIHKPSNTSLRLGFKQDLEFSDDHFYNKDLSLLSQNTLRDFFLEDLDSVRSYY
                     ASFNFSPIRYLSTNLKASYSEKTIINQDRYDFANVIPKHVNTYELGLYFKYAFKEKFL
                     QTPRGNRLSMGTKFPILYFNVIKAFPIYGTNSDYLKTEAMINYKLQWLHIGATHFTTD
                     IGMVYGNAEYSELYYGSGSYSWLNIDNSFNTMRVNEFVSDRFANLFIRHDFGNLLVKT
                     KHFAPQIVLVNAIGWGDNSRVSSTQNKSMNKGYFESGIQINNILKLSFQGYGIGIYYR
                     YGPYSLPKLIDNWAFKFNLTFVL"
     gene            12652..16041
                     /locus_tag="PKN53_05960"
     CDS             12652..16041
                     /locus_tag="PKN53_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOD88528.1"
                     /translation="MRYLFLILAFLPLIAFAQFEDDFSDGDFTNSPAWLGDTENFIVN
                     SSQQLQLYINPQAAGTSYLVTASSAINDATWEFYLKMNFNPTATNYCDVYLVSNSPNL
                     NYTTNGYFVKIGNTKKEVSLYRKDATSTALIIDGEDSRVDLTNVEVKIKVTRDSEGNW
                     TLESDILDGNGYKSEGSAFDNTYYSSAYFGIKCTYTTTRSQHFFFDDFVVTGTGFVDE
                     EAPELLTASVLNEHALLLTWNEALDAGNSLQTSNFQVSNGMGEPESATFYQSNPSQIV
                     LNFQNAFTSPNNYILQYQGVSDLSNNFSSGSISFSYITFSPGMIVFNEIMADPDPVVG
                     LPNAEYVEIYNTTNFVVDIGGWSYTHGSTTKQIPAYQMQANEYLILCKTDFVNLFTEY
                     GNVLGIEAFPQITNTGQTLILKDNEGQEIDRVSYTIGWYGTSYKEDGGWSLEKIDPTN
                     TCSPNMNWTASVDPSGGTPGRQNSVFANVVDTIPPYIINVSISSGNELLVKFSEPVDT
                     STSLVLSNYDLQPGFGTPIYAFSEINKTDEVTLQFPSSFIEDVEYSLHVRNIKDVCDN
                     IMQQQTINFMLYDAKEYEVIFSEIMAVPDPVVLLPNAEYIELYNRTDKNIDLTDWVLS
                     VGSTKKTLSGCTINANSYLVLCNQNNVNLFTGVENIVGITSFPSIPNTSGTLTLSSKS
                     DKVINTVSYSDKWYKDKFKANGGYSLEIIDLNNPCEGENNWRASNDVSGGTPGRQNSI
                     NGTNPDLYSPYPIAAELIAPDTLIVYFNEILKKESCENPENYSVENYGNPTWVSASKP
                     DFAKISMKFSQNFEQGKVYYLNIKDQVQDCAGNTVDKNTSIRFGIGDSITKDDIVINE
                     ILFNPLSGGSDFVELYNNSNKLCDLKSLWFINKDDSAAIDNSYQITTISRLLLPEEYC
                     AISKDIAFIENNYHVPFPENLFQTANLPSMADKSGNIFLIDRFTNIIDSVYYNEKQHY
                     KLLASKDGVSLERINFNRSSADETNWHSASETVGFATPGYKNSQYSDEIISESKITIS
                     PEVFSPDNDGRDDILNISYNLNSPGYTATIAVYNADGQFVKFIANNKMLATEGNFTWD
                     GFDAVNVPCPIGIYIIYIEMFSLNGEKIVEKHAAVLSRKSY"
     gene            16049..17872
                     /gene="ispG"
                     /locus_tag="PKN53_05965"
     CDS             16049..17872
                     /gene="ispG"
                     /locus_tag="PKN53_05965"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HOD88529.1"
                     /translation="MDKKYCNKYCIDTHNYERIPTRAVKVGDITIGALNPIRLQSMTN
                     VPASDIQRSVAQAKAIFDAGADFVRIATPRVMEVECLKEIKRLLHADGYKKPLIADIH
                     FNPVIAEEAARIVEKVRINPGNYIDKRANFEKIEFTDSEYKLELEKIHERILPLIKIC
                     KEYGTAIRIGSNHGSLSDRIMSRYGNTVEGMVEAAMEFIDIFVAENFYNLVISMKASD
                     VRIMTQATRLLNAKMFDRALLLPQHLGVTEAGADIDGRLKSALGIGALLSDGIGDTFR
                     VSLTESPENEIHFSKIILDKFKDKKFVYQLPTQTHNTYNPYTCNDIYPSLDFVSNKFS
                     LISEIDSDNTDIIYAENIDNLSTTKPILTTYSSWKSVKRNKNIFPYIEFTKNKLPYAI
                     DDRYYYFVGLNCYDVNKSEIKPILEKNNICLVLNYCSDNPLGETRYFYNSLKKINPDI
                     PVIVKYSAQSLNFDELSVELGLFPGVALLDNLANGLWLDIPSKEINKIEIQTDLLQAI
                     GLRYSKAEFISCPGCGRTNYDLENLMKEAKSAFSHLKGLKIAVMGCIVNGPGEMADAD
                     YGFVGSGKGKVHIFKGKNAIIKNVAQENAVEELKKVISCQK"
     gene            18033..>21179
                     /locus_tag="PKN53_05970"
     CDS             18033..>21179
                     /locus_tag="PKN53_05970"
                     /inference="COORDINATES: protein motif:HMM:NF015497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HOD88530.1"
                     /translation="MNEQDKNKTGQSDNQNKNIHSGSGNFGNRSNNFGKKESQASYSS
                     SLKNSLHTPGGIELPKGGGAIRGIGEKAEVNPVNGSGSVSIPLPITPGRSGFNPQLSL
                     SYSSGAGNSEFGLGWSVGLPEISRKTDKELPRYYDLENSDTFILSGAEDLVPVCNEEG
                     EIIDSTVGENTIRQYMPRIEGLNAKIERITKSDGYVYWRVTSKDNIVSLYGFSADARV
                     ADPENDKRIFSWKLEYTMDSLGNLAKYTYIKDNDVDYESSYLSKVEYGNDTPVSNIEA
                     IYTGNFYFSLEFVYNSNREDAFSVYRPGFEVRNTKLCSKIEMHHNIGAVQQIVKTLTL
                     SYDNSTDLNLLTKVLLTGFDSDQTPVSLPAIDFCYSLPDFPSRSKALDNKDLENMPAG
                     LSEGVSFADLWGEGISGVLIKGTKSWFYKPNLGNRKWFNSEDKNEIDLGKAFILKGLP
                     NLATENPQAAQLTDIDGDGLNEIQIMAGSIKGFHSFDESGKLSPFVAFKQMPNIDFND
                     PNLRMLDLNGDGYPDLLISDTNCFLVSYSKHKEGYGNLQRISKAIDEEKGPRVIFSEQ
                     TQTIFLADMSGDGLTDIVRIKNGSICYWPNLGYGRFGKKVQMKNAPRFDSSDMFDPSR
                     IRLADIDGSGTTDIIYLGFNQTHCWKNLSGNSWSNAILIPNFPKTDNLTNVSVFDIEG
                     NGTSALVWSTPLPGKSDRIKYIELTGGKKPFLLTHINNNMGASRRLFYAPSTKFYLQD
                     KKAGKPWITKLGFPVHVVERIEVYDEPTKSKYVSRYAYHHGYFDRKEREFRGFGMVEQ
                     WDTDDFNTQASTPLSQFPNLSEGDTYPIYTKSWFHTGFYKNADIISDLYRQEYFDAEA
                     DAWILPDTILPQGLSIQEKREAARTLKGSLLRQEIYSQDGSEKEGIPYTVSENSFKIS
                     LKQNLHKNKHAVFMVLPSESINLNYERVLEDPRIVHTLNLEYDEFGNPLKQATVAYQR
                     LTNAVAGQDKTLVSVKQNSFFNSDENTDFHRIGVAYQTKNYEIFDFETNTSKLAIEDF
                     ANFANLNKKLIQNQKTLFY"
BASE COUNT         7180 a         3461 c         3184 g         7354 t
ORIGIN      
        1 attttcaagg tgtttcagaa gtgttaaaat gcaaggcgct gaaattgagg acgaaggagc
       61 gtaccagaag cgatgcactg agcaaagccg aagtgtacgt gactgagtcc gaatattgaa
      121 agcaacgtgg ttgctgaacg aagttgaagt aaagcagttt gacacttatg agacaccgcc
      181 ttgctaaatc attattaaaa acacaaattg agtttttaaa tataaattga ttttaaaatg
      241 ttaataaatt tatgtgaata tacttacgaa aatatcgttt attgcttcca ataagcttgt
      301 atttgcattt cctgatagtt ctgatttgcg ataattctta tacaaattta aaatcttttt
      361 ttcatcttct ttatttatca aatcaagatt taccattaat gccttgttga tatcgtttgg
      421 ctcaaatttg tttaatccgt tgccgtactc tcttcggttg tcattaaaaa tttcttttga
      481 gacatttgtt aggagatagg caaaaaacaa atctactttt gattttgaaa acatattcaa
      541 ataaatgcaa tgaaaagttg taagattgct aatatttgct tcattacgaa taaaacgcag
      601 tccgttacga ttaaaaacag aaacccatat tggtgatgga gacctttttt ccaaagaata
      661 ccaaggatta cgacttgctg tcaaatacct tttgttaatt tcttccattt cgccttttgc
      721 gatgtaatta atcacagact tttcttgcaa atcatttact ttaaataaat aaactgattt
      781 atcttgtttt tttaattcct caaatttgtc ttttgtaaaa aacgagccac aaacatcaac
      841 tgattttgtt atacaaggca acaaattttt ttcagaaata ttgaattttt ctgctttact
      901 tttgctgaat gtaaaataat cattcgcacc tgtagcgatg ccacgtacca cttttccata
      961 attagtaaac gggactagat ttttaaaatg tttcgcattt tgtttttgat aatatgctcg
     1021 ccattttatt tcaggtttga gttctgaaaa tgaaactaag ttttcggatt ttctaattgg
     1081 ataagatcga attttctttt gcaatgaatt taattcatca attgttttta tatttataaa
     1141 ttcaactcca gatttttcat catcatttgc aaacaataaa attgatgctg ttgtcagagc
     1201 atcatcaaaa acattttcct caaaatcaaa aaccattaca taacgcaaga atttattttt
     1261 taacaaatag aatttcacta atttgccata atctgaattt aaaaattcag atggcactat
     1321 ataggctgcc ctgccatttt tgtttaactg ataagttgac ttcagcaaaa aaattgtata
     1381 aagattggta aaaccattta gtttcaaacc caacttttct tcaatttctt taagcattgc
     1441 tttgttgtcg taatcgtgaa acttaaaata tggcggattg caaataattc catcataacg
     1501 atttccccaa tcgttaaata tataatcctt aaaaacgatg ttagtattaa ggtctttttc
     1561 aaaaatttct ttagccttgt atagaatatt tttgtcaatt tcaaaaccgt tgataagaca
     1621 gtttttatca atttctctaa tagctctagc aaaaattcct aaaccaaatg cagggtctaa
     1681 aactgttttc agttgtttgt tccccaaaat ccattctgtc ataaattcag caacaacata
     1741 aggcgtaaaa aattgagcaa attttttgcg atgctccaac gaaacagttt gagcatattc
     1801 cttttcaggt tcattgccta ttattttaat ttttgttaca aacattaaaa atcgttttgt
     1861 ttaattccca aaatttcaca caaattattt acatctaaat aggctgctcc acctttaaat
     1921 gttacataaa acaacaaacg ccctctattc atttcttttc gcacactctt atgttcgggt
     1981 tcatcaactt tataagctat ttgaatgcca cttttagaat taattttgat tattgtttta
     2041 ttttgatttt tagtatcggc ttctactgaa aatacaacac cttctttttc ggggtctgaa
     2101 ataattgtaa gaatctgttc gaaagaaatg ccatacacct tgtcaaagaa aacttggaaa
     2161 taaatatgcg gaacattaaa agtttcaatc catttgtaaa ctactttaat gtcttcaacc
     2221 tttggagtaa ttgataaaaa atctcttttt tgaatttctt ttattgctcg ttttaattct
     2281 ttaaaatgat tattcaactc tattaatctt tcagacgaac tccaaccagg aactttaaaa
     2341 tcagcaacac ttaatgtttc tactgagatt gatttaagaa taggaatgta tttacatcgg
     2401 tttggctgtt ctaacaaatc taaatattca gcaagaattt tgtctcttgt ttgcaaagca
     2461 aattgagaaa aattttctgt acgaatttgc attgccttct cataacggtc aattaaaaaa
     2521 gcactagaac ggacttcaat tccagcaata gcttttttga cataatctgc aatagtacta
     2581 tgtgggattt ggctaatatc gtaccctaat tgatcattaa aatcagtctt tttgaaaatc
     2641 agcaaatcag gtctttttcc aatggtatct aactcgtttt gaaattcttg ataaaattcg
     2701 tcaaagccct catcacctgc aattaaatcg tctgacttac catattttat agcaacaaag
     2761 tttttagaaa catcatttat cgcttttgca acaaggcttt ctgcccaatc accctgttct
     2821 ttattagtta taaagtttga tgatgcctgt gtaggtattc ttgctaactc acgtggttgc
     2881 gaaaagtcaa caagactcag agggacattt ttaattaaat ttcttatctg ctcgtaataa
     2941 ttcattttca ttttaatatt agcacatcat ttaaacgatt tttaccaaat gtttctattt
     3001 ttcatttgtt caaaaataat aaatattttt tgatttgtgt attaatttta acccaagttt
     3061 tttacaccaa ttttaaatgg aataaaaata atctctgcct taatccccca ccatttgttt
     3121 atacatcaaa tacttaattt tataattaat ttttgcgtca gtatattggt cttttgattg
     3181 ttgaagtagt gtttgtgatt gcaacagttc tgaaacgctt accattcctg cttgataatt
     3241 atcatttact aactttaaat ttgcttctgc ctcatcaata gttttttcta ataattgtac
     3301 ttgcgaatat gcctcaacta attcattcca agcaagttgc atttgtaaca ttaatttttc
     3361 ttgcatgtca tcacgttttt gtgcagcaat ttcacattta gttttatgat atttcatgct
     3421 atatttattc tgccaccagt ctgatatagg tatagaaact gtgaaaaaac caattaaatt
     3481 tgcattatgt ttttctaaaa aattattata catataccct actcctactg ctgcttgagg
     3541 caaaacttcg cccaacttca ttcttttttg caagttttca gcttctacac ttaaattcaa
     3601 taatttatat tctattctgt ttggtaatac ttctgcgtga acttttttta tttgctctgg
     3661 attttcaatt gttgcaatat tatcaacaaa tttaatacta tcgttatatt ctatacctat
     3721 ctgttgacat aaagccattt tagctaattt tattccatta ttcaatctca ataaatttga
     3781 atttatttca tgtattttta attttactgt tagcacattg ttgtttgtta ttaatccggc
     3841 ttctgctgca cctgttacat ctttataaat tgtatcaagc atagatttag caacattaat
     3901 agtggttaat ttttcttcta atgatattat ttgccaaaaa gccgattctg ttttcagtaa
     3961 aatatctttt aaatacaatt ctatttgata ttctgaagca gtaattccta aattagccaa
     4021 tttatttcca gtaacaattc tacctcctgc aaatactggt tgaacagcag taattccagc
     4081 aaacacacca tctttaagtg ctcgtgctgt taaatcagta ttttgaaaat aaactccata
     4141 agtattgtat aaagttgtta aaatatcgtt ataatgctga ttttcaaggt ataattcaat
     4201 gtctatatca tttatatcta cgtcaataag atgtttgttt gcaataaatc cacctgaagt
     4261 tgctgagact ttaggaaaat agttagtaaa agcttgtttt ttagtatatt ttgctagttc
     4321 cacttctaat ttttgagaag aaatatctat attattttct attgctagag ctttacaaga
     4381 atctaaggaa tatacattag tttgtgcatt tactattgtt gcattaaaaa taattaaagt
     4441 taatatggat attaaaatat ttttcatttg cttgctttta ctgggctttt tttattaaaa
     4501 actagccaat acataactgg caatacagtt atcacggcaa ttaaagagaa gattgttcct
     4561 acaaatatta caattcccat aggcatccaa agtgtacttt tgcttaatat cattgggatt
     4621 acacctactg cggttgtcat tgatgttaaa aatataggtc tcattcttct tttaccagct
     4681 tctaaacctg cttgatatgc tgaaagttta tgaactgtgc gttgttcttc ggcatattcg
     4741 tacataatta ttacatttct aaccataatt cctattaggt ttactattcc caaaacagat
     4801 gttaagctta aatcttgttt aaacaaatag agacctacaa acaccccaaa catacataat
     4861 gcatttgccg ataaagataa aacagatatg ctaacgtgtt taaaagtcat taataaaaat
     4921 gtgaaaataa ctaccaatgc tgccacaata caggtgatta cttctggtac agtattttta
     4981 tttacttctg acaatccacc atattctagt tttaaattgc ttggcatttc aggaagtatt
     5041 tcatttttaa cacgtttttt tatcttttta attactacag attctttctt atcaaatttc
     5101 atatctgcac ttactgtaat gcaatatact ccgtttcgtc ttactaactg tgatatttgc
     5161 cagtcgggag caatttttgc aacttgacgt aaaggcaccc atgtccccgg tattactgta
     5221 ggaacaattt catttttcaa gtcttcgaaa tcagcccaat tattatctaa atttcttatt
     5281 agttttactg ctacaggata atcttcttcc caaatagttg ttagaggcaa accatgaaag
     5341 tttatagcta aactagttga caaatacgat tttgtaattc ctagtcgcat agcttcttcg
     5401 ggcaagattt caattttaac aaaaggcata gtgccattaa aatcattatg gatccatgtt
     5461 aagtcgtcat caaaatcatg cagtatagtt tcaacttttt gagcttgttc taacaattta
     5521 ctaacatcat cgccgctaat tctaatttct ataggattgc taacagcttg aaaatccatt
     5581 tgtttaaaac gaacataagc atttggaaaa taattagaat acataggctc caatttttta
     5641 agtaacgaaa ttgttgcctc atctgatttt gtttttacaa taaattgtgc ataattactt
     5701 cccggaagct ttggagcgta ggtcatatga aaccttggcg aacttgttcc aataaattct
     5761 gtaacagata ctacatcttc ttctttttca ataatttttc gtaagctatc acttaacatt
     5821 gcggtttgtt cgagagcagt tccttctggt aaaaatattt ctaccgcaaa agtatctcta
     5881 tctgccttag gcatcatttg tactggtgta atcattaaca agaaaactgc tacaccaatt
     5941 gacattaatg ccacaaagat tgttgttcta ggttttttaa agcaagaagt taaaagtttt
     6001 tcgtagccta tttgtaaatg atgaaaaaac ttattttgca atctagctat tagaccaact
     6061 ttatcagttt ctgatggttt ttttataaat aaaaattgat aataaggaat tacgaaaaga
     6121 gctaacaata aagatgccat taaagctatt gtcattgtag gtgggaaaga cttaacaaaa
     6181 tcgctcattt ctccagtaaa aattcctaat ataggaaaaa acaaagcaca aatagctatt
     6241 gttgccattg ttagtgatgg tgcgtatgtt ctagcacttt ctaccgatga ataccaacgc
     6301 gaatgacctc tattcaaatt atcaatatat ccatcaataa ccacaataga attatcaacc
     6361 accattccta atacgacaat aagtactgcc agcgtaaccg tatttaattc tattccaaac
     6421 aaatacataa aagctaaagt tattgcaata ctcacaggga tagatgtagc tgctactaat
     6481 gcagaacgta gtgggaagag cataagcata accacaatta caatgagtat agcaactcct
     6541 aaatctgtaa gaaatgatct aacagacgag cctactattt cgggttggtc ggttatacgt
     6601 tctattttta tactttgagg aatagtattt ttaaagttgt ttaatatttc atctacttct
     6661 ctaccaaact ctacaacatt gttaccaggt ctcatttcta tagacacaac taaggtttta
     6721 ttgccattag aacggatata gctagcatca agtggatagc ttctttttac tgtggctacg
     6781 tcttttaatc tcacaacttt tccgcctggt tccgacaata ttatttgctc tgagagttct
     6841 tcttcacttc gatatgggct cgtaatatga attggcaaac ttatgtcttc attagttatt
     6901 gcaccacttg ccatttgagc tccttgcgta aataaattta gcaataatgt ctccctacta
     6961 attccatatg ctgctatttt ttcaggctca acgtgtatat gtatttcttc gttttgtttg
     7021 ccatgaactt ttatatttcc gatagcatct attgttcgga gctggtctgt tagtttcgag
     7081 acataagtat ctaattctct ataagttttg tctgcagatt ctattgtaat taataatgaa
     7141 gtagtatttc caaaatcgtc attaacaata agtgctaaaa ctcccgttgg caacatagct
     7201 ttaaagtctt ttaagccgtg tcttatttta gaccaagttt ctgtttgatt ttttactgaa
     7261 ttatttaatt ttacaaaaac aaatactaaa ccgtctcgtg agtatgaata agtttcttgt
     7321 ttattaattt cttgaaatgt aaacaaatac tcttctaaag gttttgttaa ttgctctaaa
     7381 acttcgttag gcgtagctcc gggatatacc ccaacaatca caccttgacg aatagtaaaa
     7441 acagggaatt cttgtttatt cattttaaat aaaccataaa ttccaaatat cacaaacact
     7501 atcacagcta aagttatcaa actgtgataa cgcatggtat tgctaaatat gtttttacct
     7561 aacttcattg aattttaact ttgcagcctt cacttacttt tgtaaatccg tttacaataa
     7621 ttttttcatt ttcttttatt ccagaagtaa taataacgtg attatcaatc aaatcaccta
     7681 cttcaacaat tttacgtgta gattttccat cttcaactac ccaaacaaat tttgagccgt
     7741 cattagataa ttgcactgca ttaacaggta ctttaaagcc tttatatttt ttcccggtag
     7801 ttatattagc acggcaagtc attcctggca ttaatcgctt atcagtatta tcaataccaa
     7861 ttttcacaac ataactatga gataatttat cagcactaac gcctttttct ataaccttac
     7921 ctttaaatgc ttcttcagac actgccgaaa ttctcacctc agcattttga cctacagata
     7981 tttttgaaat ttctttttct ggaacattaa cactagcttt aactatggta atatccatta
     8041 atttaaaagc tggttgatat ggtaaaatgt tcattcctgg cgacaccatt aactctccaa
     8101 caactccaga ctgtggagat cgcaaaacac aattgtctaa attacgcctt gcaatttttt
     8161 cagaagcacg tgcttgtgat agttgcgttt gaatttctat ccatttaact tctgctaaac
     8221 ttccattatt atatacttgc tctgctcttt tataaccatc ttctgctcta tctaaggtgg
     8281 actttgccaa ctcataagca ttttgcgaag cttctttatt tatttctgaa acgacttgtc
     8341 cttttgcaat actttgtcct tctgtaacaa atatactttc aatattaccg cctacttcaa
     8401 aactaagcaa tatagaagaa ttttcttcaa ttactcccat atagtcatgc agtttatcga
     8461 aggaatcaat agaagcctcc attacattta ctgctatttc cccattatta gaattagact
     8521 tatcgttgtt acaactactt ataaataaaa taattataaa aaagaatata agtttaaatt
     8581 tatagtttgt catataattt atttgatttt agtccaatat tcagttgctc caaacattgg
     8641 atgaccaaca tatcctcgta attttaattt tgtttttgtt tcaaagttca tttttgcagt
     8701 ataagtctta ccatttgttg gattataaac tttcccgtca acccattctc cttttttaaa
     8761 tgtaaaacca tataaaatag tcatattatc acaacgcaca tggcgtttag aaggatcagg
     8821 atttaaaaca tcgcaataag gttttccatc tttatcaaga ggtttttcta accaaataac
     8881 ttttccgcta taagtattat catgaagttt aaaaatttca attttacaat tttgttttgt
     8941 taatttatca taagtaagat atactccgac aatatcatca gctttaactt gcgaatataa
     9001 attattagaa aatataaaaa ctaataaaaa tatacccaaa atctttccaa ctattttcat
     9061 cataatatta atttatttat ttttttcaga aattgttata acatcacttt gtttgattgg
     9121 acctttcgct ttataagtat caacatatcc aaagtaaaaa tcttctatca caattctgtc
     9181 ttcatatcgt ttaccaacag gggcactata aatatttgtt tccgaagata cggtatcaag
     9241 accagaaata aagatttctg agataggaat agtatcaaaa attattttat tctcatcatc
     9301 agcagaaatt gttgcatagg tttttgtaat attacctgtg atttctgttt taataaccat
     9361 aactctattt tcttcaccaa tgtcaataat atccatctga cctaatttat tagtaatttt
     9421 caaagcaaca tcactagtat cgccttctac agtaatagaa cctgatgttt tataagtaaa
     9481 atctcctaaa aaatttttta caggtttatt atcacatgaa taaaataata tagtaaaaaa
     9541 acagaacagc aataatggta atttattttt tttcatcata tattttatat ttataaatta
     9601 acacaaaaca acgtttaata ttatgtttat tttcttaaaa atttcataaa aatttcatct
     9661 caaaataaac aaaaacattt ccctcaaaaa tataataatt ttttgaattt tctactaatt
     9721 aataagtttt ttactactgt tttattttaa taattgtttt gtcttattat ttttgaaaat
     9781 tatttaattt tgatgaatta cttaagatta atactgagtt tcatattttt atatttttta
     9841 actacaaatt tatattctca aaataaattt gctggtcgag ttcttgatgc aaaaactaat
     9901 gaggcactag ctttcgtaaa cattacctac aattcaaaca actatggaac tactacagat
     9961 ttagatggtt attttacaat taattcagct gacaaaattg agtttttaaa actttcatac
    10021 ttgggatata caacaaaact tattacgaaa gacgaaattc aaaataaaaa atatattgaa
    10081 attagtttag aacaagatgc tttctaccta tctgaagtta ctattttgcc gggaataaac
    10141 ccagctcacc gaattattga tttagcaata gaaaattcaa ataaaaataa tcccgaaaaa
    10201 atacgctctt tttcttatgt ctcgtacaac aaaatgattt ttacagttga tatgagcaat
    10261 attcaagctg acacaacaaa aaccaatgaa acaaagacag atacaacaga taaaaaaact
    10321 ttaagagaat tttttgatga acaacatatc tttattaccg aatctgtaac tgaacgaaga
    10381 tttcgctatc ccgaccaaaa taaagaaact gttttggctc ataaaatgtc tggtttaaaa
    10441 aatcctgcat ttactttact tacaactcaa tttcaatctt tttcttttta taaaaatttt
    10501 atcagcctac tcgataaaaa atatttaagc cctattagca gagggagtac ttcaaaatat
    10561 tttttccaaa tagaagatac aatgtataac gaaaatgccg acacaatttt tataatttca
    10621 tatcgtccgt caaaagggaa aaattttgaa ggtttgcaag gagttttgca tataaactca
    10681 aatggctacg ctttacaaaa tgtaagtgca aaaccttacg aaccaacagg tagttttaat
    10741 ataaacattc agcaaaaata tgaattgata gactctattc agtggtttcc tgtgcagtta
    10801 aatacagatt tagttttcaa tttttttcaa gttggggaaa atgaagcagt tatatcatct
    10861 ggaaatagcg atactaaaag ctcttcttac gctcctttag ttggtattgg aaaatcatat
    10921 attagcgaca taagtttaaa cccagattac aaacgccgag agttcaatca attaagtgta
    10981 gaagttgaaa aaaacgcaca taaaaaagac aaggattttt gggattatta ccgttacgaa
    11041 aaactaagcg ataaagatat taaaacctat caagtgatcg acagcattgg cagagaaata
    11101 aaccttgaca aaacaatgga attaatggaa gttattgcaa caggttatat tccaattaag
    11161 tttgttaatt tgaaccctgc atctatttta tggtataata aatatgaagg ttatagatta
    11221 ggcttagccc ttgcaacaaa tcataaattg gtaaaatttt tatccttagg tggacatttt
    11281 ggttatggtt ttaaagataa agcttggaaa tacggtgctt atatagattg gtttattcac
    11341 aagccttcga acacaagcct aagacttggt tttaaacaag atttagaatt tagcgacgac
    11401 catttttata ataaagattt gtctttgctg tctcaaaata ctttaagaga ttttttctta
    11461 gaagatttag attctgtgcg ttcttattat gcaagtttta atttttcacc tattagatat
    11521 ttaagcacaa acttaaaagc atcatattct gaaaaaacaa ttatcaacca agatagatat
    11581 gattttgcca atgtaattcc aaagcatgta aacacttatg agttaggact atattttaaa
    11641 tatgctttta aagaaaagtt tttacaaaca cctcgtggca atagattgag catgggaaca
    11701 aaattcccaa ttttatattt taatgtaata aaagcttttc caatatatgg aacaaattca
    11761 gattatttaa aaactgaagc catgataaac tataaattgc aatggttgca cattggagcg
    11821 acacatttca caactgatat tggcatggtg tatggaaatg ctgaatattc agaactttat
    11881 tatggctcag gttcttattc gtggttaaat atcgacaata gttttaacac catgagagtt
    11941 aacgaatttg tctctgatag atttgccaac ctttttataa gacatgattt tggaaatttg
    12001 cttgtaaaaa caaaacactt tgcaccacaa attgttttag taaatgctat tggctggggc
    12061 gataattcaa gagtttcaag cactcaaaac aaaagtatga acaaaggtta ttttgaatct
    12121 ggaattcaaa ttaataatat tttaaaatta agctttcaag gatatggaat aggaatttat
    12181 tatcgctatg gtccttattc tttgcctaaa cttattgata attgggcgtt taagtttaat
    12241 ttgacttttg ttttataaaa ggcgctttat aagtattcaa ctgctttact tcaacttcgt
    12301 tcagcaacca cgttgctttc gatgttcgga ctcagtcacg tacacttcgg ctttgctcag
    12361 tgcaacgctt catgtacgct ccttcgtcct caatttcagc gccttgcatt ttaacacttc
    12421 tgaaacacct taaaaattca ttgtttcaaa atttgttttg agacagcctc ttttttcttc
    12481 tgctcgctca gatttttgtg gaaatttccg aaagagcgta aacgagcttg ccaaacaatt
    12541 tcaatcaccc catttttaaa ttgctcaaaa atatttttta tttataaaac ttatttatac
    12601 tattttattt atctttacgc attattttta agtaacgtaa acaatattat aatgagatat
    12661 ttatttttaa tattagcttt tcttccgctt attgcttttg cacaatttga agatgatttt
    12721 tctgatggag attttaccaa ttccccagct tggcttggcg atacagaaaa ctttattgta
    12781 aattcctcac agcagttaca attgtatata aatccacaag ctgcaggtac atcttatctt
    12841 gtaacagcat cgtctgcaat taatgatgca acatgggaat tttacttaaa aatgaatttt
    12901 aatcctacag caacaaatta ttgtgatgtt tatttagttt ccaactcgcc taatttgaat
    12961 tatacaacta atggatattt tgtaaaaatt ggaaatacaa aaaaagaagt ttccttatat
    13021 cgcaaagatg ccacaagcac agcattaatc attgacggcg aagatagcag agttgaccta
    13081 actaatgttg aagtgaaaat aaaagttact cgtgatagcg aaggaaactg gactttagaa
    13141 tctgatattt tagacggaaa cggatataaa tctgaaggtt ctgcttttga caacacatat
    13201 tatagttctg catattttgg tataaaatgt acttatacaa caactaggtc tcaacatttc
    13261 ttttttgatg attttgttgt aacaggaaca ggctttgttg acgaagaagc tcctgaactt
    13321 ttaactgcat cggttttaaa cgagcatgct ttattactaa cttggaacga agctttagat
    13381 gcaggcaact ctttgcaaac cagcaatttt caagttagca atggcatggg cgaacctgaa
    13441 tctgctacat tttatcaaag caacccaagc caaattgtat taaattttca aaacgctttt
    13501 acttcgccaa ataactatat tttgcaatat caaggagtaa gtgatttaag caacaatttt
    13561 agttcaggct ctataagttt tagttatata acattttcgc ctggcatgat tgtttttaat
    13621 gaaattatgg ctgaccccga ccctgttgta ggtcttccaa atgctgaata tgttgaaatt
    13681 tacaatacta caaatttcgt ggttgatatt ggcggttggt catatactca cggaagcaca
    13741 acaaaacaaa ttcctgctta ccaaatgcaa gcaaacgaat atttaatttt atgtaaaacc
    13801 gattttgtta atctttttac tgaatatggt aacgtacttg gtatagaagc ttttccacaa
    13861 attacaaata ctggacagac tcttatttta aaagataatg aaggacaaga aattgataga
    13921 gtttcttaca ctataggctg gtatggaaca agttataaag aagatggtgg ctggtcttta
    13981 gaaaaaattg acccaacaaa tacttgctcg ccaaatatga actggactgc ttctgttgac
    14041 ccaagcggag gtacaccagg aagacaaaac tcggtttttg caaatgttgt tgatactatt
    14101 ccaccatata ttataaatgt ttcgataagc tctggaaatg aactacttgt aaaattttca
    14161 gaacctgttg acactagcac aagtcttgtt ctttcaaact atgacctaca accaggtttt
    14221 ggaactccaa tttatgcttt ttctgaaatt aataaaaccg atgaagttac attacaattt
    14281 ccatcatctt tcattgaaga tgtagaatat tctttacatg taagaaatat taaagatgtt
    14341 tgcgataata tcatgcagca acaaacaata aattttatgc tttacgatgc aaaagaatat
    14401 gaagttattt tttcagaaat tatggctgtc cccgaccctg tagttttatt accaaatgca
    14461 gaatatatag agctttataa tcgcactgat aaaaatattg acctaactga ctgggtttta
    14521 agtgttggtt ctactaagaa aactttaagt ggctgtacaa ttaatgcaaa ttcctatctt
    14581 gttttgtgta atcaaaataa tgtaaattta tttacaggag ttgaaaatat tgtagggata
    14641 acaagttttc caagcatacc aaacactagc ggtactttaa ctcttagttc aaaatctgat
    14701 aaagttataa atactgtttc ttattctgac aagtggtata aagataaatt taaagcaaac
    14761 ggtggttatt ctttagaaat tattgattta aacaatcctt gcgaaggcga aaacaattgg
    14821 cgagctagca acgatgtttc tggtggtact ccaggaaggc aaaattctat aaacggtaca
    14881 aatcccgacc tttattcccc ctatcctatt gctgctgagc taattgcccc agatactcta
    14941 attgtttatt ttaatgaaat attaaaaaaa gaatcttgcg aaaatcctga aaattattct
    15001 gtagaaaatt atggaaatcc tacatgggtt tcagctagca aacctgattt tgcaaaaata
    15061 agcatgaaat tttcacaaaa ttttgaacaa ggaaaagttt attatctaaa tataaaagac
    15121 caagttcaag actgcgcagg caatactgtt gataaaaata ctagcatacg ctttggaatt
    15181 ggcgactcca ttacaaaaga tgatattgtg ataaacgaaa ttttattcaa tccacttagc
    15241 ggaggaagcg attttgttga actttataat aattctaata aactttgcga tttaaaatct
    15301 ctttggttca taaataaaga cgattctgcc gcaatagata acagctatca aattacaaca
    15361 atcagtcgtt tgctgcttcc tgaagaatat tgtgcaattt ctaaagatat agcatttatc
    15421 gaaaacaatt atcatgttcc tttccctgaa aatcttttcc aaactgctaa tcttccttct
    15481 atggctgata aatctggaaa tattttcttg attgatagat ttacaaatat tattgattcg
    15541 gtttattaca acgaaaaaca acactataaa ttattagctt caaaagatgg agtttcttta
    15601 gaaagaatta attttaatag aagttctgct gatgaaacaa attggcattc tgcgtcagaa
    15661 actgttggtt ttgcaactcc tggttataaa aactcgcaat attctgatga aattatatct
    15721 gaatcaaaaa ttactattag tcctgaagtt ttttcgcctg ataatgacgg aagagacgac
    15781 attttaaata ttagctataa tttaaacagt cctggttata ctgctacaat tgctgtttat
    15841 aacgctgatg gtcagtttgt taaatttatt gcaaataata aaatgctagc cactgaaggc
    15901 aactttactt gggacggttt tgatgctgtt aatgttccct gtcctattgg catttatatt
    15961 atttacatag aaatgtttag tcttaatggc gaaaaaattg tagaaaaaca tgcagcagtt
    16021 ctaagccgaa aatcttacta attttaaaat ggataaaaaa tattgcaaca aatattgtat
    16081 tgacacacat aattacgaac gcattcccac acgtgcagtt aaagtaggcg acataactat
    16141 tggagcttta aatcctattc gtttacaaag tatgacaaat gttcctgcaa gcgacattca
    16201 aagaagtgta gctcaagcca aagcaatttt tgatgcaggt gcagactttg taagaattgc
    16261 cacaccaaga gttatggaag ttgaatgttt aaaagaaata aaaagacttt tacatgctga
    16321 tggctacaaa aaacctttaa ttgccgatat acattttaat cctgtaattg cagaagaagc
    16381 cgcacgtatt gttgaaaaag ttagaataaa tcccggaaat tatattgata aaagagcaaa
    16441 ttttgaaaaa atagaattta cagattctga atataaattg gaattagaaa aaattcacga
    16501 aagaatatta cctttaataa aaatttgcaa ggaatacgga actgcaatta gaataggttc
    16561 gaatcatggc tcgctttctg acagaataat gtcgcgttat ggcaatactg ttgaaggaat
    16621 ggtagaagca gctatggaat ttattgatat ttttgttgcc gaaaattttt acaatcttgt
    16681 tatttcaatg aaagccagcg atgttagaat tatgacacaa gctacaagat tgctaaatgc
    16741 taaaatgttt gatagagcct tactcctccc tcaacatttg ggcgttaccg aagcaggtgc
    16801 cgatatagat ggaaggttaa aatctgcact tggaattgga gctttactct ctgacggaat
    16861 tggcgacact tttagagttt cactaacaga aagccctgaa aatgaaatac atttttctaa
    16921 aattatttta gacaaattta aagacaaaaa gtttgtatat caacttccca cacaaactca
    16981 caatacttac aatccatata cttgcaacga catatatcct tctttagatt ttgtgtcaaa
    17041 taaattttcg ctaatttccg aaatcgactc cgacaataca gatataattt acgctgaaaa
    17101 cattgataat ctttcaacta caaaaccaat tttaacaact tactcatcgt ggaaatctgt
    17161 aaaaagaaat aaaaatattt ttccatatat tgaatttacc aaaaacaaac taccttacgc
    17221 aatagatgat agatattatt attttgtagg gcttaattgt tacgatgtaa ataaaagcga
    17281 aataaaacct attttagaaa aaaacaatat ttgcttagtt ttaaattact gctccgacaa
    17341 tcctttgggc gaaacacgat atttttataa ttccttaaaa aagataaatc ctgatattcc
    17401 tgttatagtt aaatattcgg ctcaatcttt aaattttgat gagctttctg ttgagttagg
    17461 actttttcct ggagtagcgt tgcttgataa tcttgctaat ggattgtggt tagatattcc
    17521 aagcaaagaa ataaataaaa ttgaaataca gacagatttg ttacaagcaa taggtttgcg
    17581 atactcaaaa gcagaattta tttcttgtcc gggttgtggt cgcacaaatt atgatttaga
    17641 aaatttaatg aaagaagcta aaagtgcatt ttctcattta aaaggattaa aaattgccgt
    17701 tatgggttgc attgtgaatg gacctggcga aatggctgat gcagattatg gttttgttgg
    17761 ctcaggaaaa ggaaaagtac atatttttaa aggcaaaaat gctataataa aaaatgttgc
    17821 ccaagaaaat gctgtggagg aattgaaaaa agtgatttct tgtcaaaaat aattattaca
    17881 ataaacttaa tttttaattc aatgtttaaa atttttttgt aactttggaa aaaaacacaa
    17941 atgcaaaatt tgcactgtga tttaataaat tttgaagtag aagtagggat taggcggttg
    18001 gttttacaat cacaacattt tataaaaaaa ttatgaacga gcaggacaaa aataaaacag
    18061 ggcaatcaga caaccaaaac aagaatattc attctgggag tggtaatttt gggaatagaa
    18121 gcaataattt tggaaaaaaa gaatctcaag cctcttattc ttcttcttta aagaactctc
    18181 ttcatacgcc tggtggtatt gagttgccaa aaggtggcgg tgctattcgt ggtattggcg
    18241 agaaagctga agtaaatcct gttaatggtt ctggttctgt gagtattcca ttgccaataa
    18301 ctcctggtcg ttcgggattt aatcctcaac ttagtctttc gtatagttct ggagcaggaa
    18361 acagcgagtt tggtcttggt tggagtgtgg gtttgcccga aatttctcgt aaaaccgata
    18421 aggaattgcc acgctattac gatttggaaa attccgatac ttttattctt tcaggagctg
    18481 aagaccttgt gccagtttgt aacgaggaag gcgaaattat tgattctaca gttggcgaaa
    18541 acaccattag acaatatatg ccaagaatag aaggacttaa tgcaaaaatt gaacgcatta
    18601 caaaatctga tggctatgtt tattggcgtg taacaagcaa agataatata gttagtcttt
    18661 atggtttttc ggctgatgct cgcgttgccg atccagaaaa tgataaacgc atttttagct
    18721 ggaaattaga atacacaatg gacagcttag gcaatcttgc aaaatatacc tatataaaag
    18781 ataatgatgt tgattatgaa agttcgtatc ttagtaaagt tgagtatggc aacgacacgc
    18841 cagtaagtaa tattgaagca atatacacag gcaattttta tttttcgctt gaatttgttt
    18901 acaattctaa tagagaagat gctttttctg tgtatcgtcc tggttttgag gtgcgtaaca
    18961 ctaaactttg cagcaaaata gaaatgcatc ataatatagg tgcagttcaa caaatagtaa
    19021 aaactctaac actttcttat gacaattcta cagatttgaa tttgcttaca aaagttttgt
    19081 taacaggatt tgattccgac caaacgcctg taagtcttcc tgcaatagat ttttgttatt
    19141 ctttgccaga ttttccttca aggtcaaaag cattggacaa taaagatttg gaaaatatgc
    19201 cagcaggact aagcgaaggt gtttcgtttg ccgacctttg gggcgaaggc ataagtggag
    19261 ttttgataaa aggcacaaaa tcatggtttt ataaaccaaa tcttggaaat cgcaaatggt
    19321 ttaatagtga agataaaaat gaaatagacc ttggtaaagc ttttattttg aagggcttac
    19381 caaatttggc tacagaaaat ccgcaggcag cacagttaac cgatattgat ggcgatggac
    19441 ttaacgaaat acaaattatg gcaggcagta ttaaaggttt tcattctttt gacgaatctg
    19501 gaaaactttc gccatttgta gcatttaagc aaatgccaaa tattgatttt aacgacccaa
    19561 atctaagaat gttagacctt aacggagacg gctatcccga ccttttgatt agcgatacaa
    19621 actgcttttt agtttcatac tcaaaacata aagaaggcta tggcaatttg caacgcataa
    19681 gcaaagctat tgatgaagaa aaaggaccac gagtaatttt ttctgaacaa actcaaacaa
    19741 tttttcttgc cgatatgagc ggagacggat taacagatat tgttcgcata aaaaatggca
    19801 gtatttgcta ttggcccaat cttggatatg gcaggtttgg taaaaaagtg caaatgaaaa
    19861 atgcaccacg tttcgacagt tccgatatgt ttgacccctc acgcatacgt ttggcagata
    19921 ttgacggaag cggaactacc gatattattt atcttggatt taatcaaaca cattgttgga
    19981 aaaatctttc aggaaactcg tggagcaatg caatacttat acctaatttt ccaaaaacag
    20041 ataatttaac caatgtgagt gtgtttgata ttgaaggcaa tggcacatcg gctttggtat
    20101 ggtcaacacc gcttcctggc aagagcgaca gaattaaata cattgagcta acaggaggca
    20161 aaaaaccttt tttacttaca catataaata ataatatggg tgcaagcaga agattgtttt
    20221 atgcaccttc tacaaaattt tatttgcaag ataaaaaagc aggaaaacca tggattacaa
    20281 aattaggatt tcctgtgcat gttgttgaaa gaatagaggt ctatgatgaa ccaacaaaaa
    20341 gcaaatatgt tagtcgctat gcttatcatc atggatattt tgacaggaaa gaaagagagt
    20401 ttagaggttt tggcatggtt gagcagtggg acaccgatga ctttaatact caggcttcga
    20461 ctcctcttag ccagtttccc aatctgagtg agggcgacac ctatccaatt tacacaaaat
    20521 catggtttca tacaggattt tataaaaatg cggatataat ttctgatttg tacaggcagg
    20581 aatattttga tgcagaagca gatgcttgga ttttgccaga tacaattttg ccccaaggct
    20641 tgagtattca agaaaaacgc gaagctgccc gcacacttaa aggaagcctt ttgcgacaag
    20701 aaatttattc tcaagatggt tctgaaaaag aaggaattcc atatactgtt agcgaaaatt
    20761 cttttaaaat ttctttaaaa caaaatttgc ataaaaataa acatgcagtt tttatggttt
    20821 tgccaagcga aagtattaac cttaactacg aaagagtttt agaagaccca agaatagtgc
    20881 atactttaaa tctcgaatac gatgaatttg gaaatccatt aaaacaggcg actgttgcat
    20941 atcaaagatt gacaaatgct gttgcaggac aagacaaaac tcttgtttct gtaaaacaaa
    21001 actcattttt taattcagat gaaaatacag attttcaccg aataggagtt gcatatcaaa
    21061 caaaaaacta tgaaattttt gattttgaaa caaatacttc aaaacttgca atagaagatt
    21121 ttgctaattt tgcaaactta aacaaaaaat taatccaaaa tcaaaaaaca ttgttttac
//
LOCUS       DAOPNX010000041        20700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_3177_length_20700_cov_6.627658, whole genome shotgun sequence.
ACCESSION   DAOPNX010000041 DAOPNX010000000
VERSION     DAOPNX010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20700
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3177_length_20700_cov_6.627658"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /gene="rpsS"
                     /locus_tag="PKN53_05975"
     CDS             <1..251
                     /gene="rpsS"
                     /locus_tag="PKN53_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOD88531.1"
                     /translation="KGPFFDFNLWRKVIAMDASGKKNAIKTWARASVIPPEFVGHTFA
                     VHNGNKFIPVYVTENMVGHRLGEFAPTRTYRGHGGKKK"
     gene            266..691
                     /gene="rplV"
                     /locus_tag="PKN53_05980"
     CDS             266..691
                     /gene="rplV"
                     /locus_tag="PKN53_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOD88532.1"
                     /translation="MGARKRIKAEQRKEAKKHISYAVLRNCPSSPRKMRLVADLVRGQ
                     KVDRALDILHFSPKAAARDVEKLLKSAVSNWQAKNEGQRIEDVELYVTEITVDEARPL
                     KRIKPAPQGRAYRIRKRSNHVRIVVDTKREKELLNEENA"
     gene            697..1341
                     /gene="rpsC"
                     /locus_tag="PKN53_05985"
                     /pseudo
     CDS             697..1341
                     /gene="rpsC"
                     /locus_tag="PKN53_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656540.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            1437..1862
                     /gene="rplP"
                     /locus_tag="PKN53_05990"
     CDS             1437..1862
                     /gene="rplP"
                     /locus_tag="PKN53_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOD88533.1"
                     /translation="MLQPKKSKFRRQQKGRMKGNAQRGNQIAFGSFGIKALESAWITG
                     RQIEAARIAVTRKMQRQGQIWIRIFPDKPVTSKPAEVRMGKGKGAPEGFVARVTPGRI
                     LFEAEGVPFDLAQEALRLAAQKLPIKTKFIVRHDYDYNK"
     gene            1874..2074
                     /gene="rpmC"
                     /locus_tag="PKN53_05995"
     CDS             1874..2074
                     /gene="rpmC"
                     /locus_tag="PKN53_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOD88534.1"
                     /translation="MKNKEIRGLSTKEIADRLVSEQHNLTKLRINHAVSPLDNPQRIK
                     EVRRLIARLKTELRNRELTEIK"
     gene            2084..2341
                     /gene="rpsQ"
                     /locus_tag="PKN53_06000"
     CDS             2084..2341
                     /gene="rpsQ"
                     /locus_tag="PKN53_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOD88535.1"
                     /translation="METRNLRKERIGIVVSNKMDKSIVVAVKRKEKHPKYGKFVNKTK
                     KFVAHDETNTCDIGDTVQIMETRPLSKTKRWRLINIIEKVK"
     gene            2344..2712
                     /gene="rplN"
                     /locus_tag="PKN53_06005"
     CDS             2344..2712
                     /gene="rplN"
                     /locus_tag="PKN53_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOD88536.1"
                     /translation="MIQQESRLNVADNSGAKEVLCIRVLGGTKRKYASIGDKIVVSVK
                     SALPGGDVKKGAVAKAVIVRTKKEVRRQDGSYIRFDDNACVLLTAAGELRGTRIFGPV
                     ARELREKQYMKIVSLAPEVL"
     gene            2730..3056
                     /gene="rplX"
                     /locus_tag="PKN53_06010"
     CDS             2730..3056
                     /gene="rplX"
                     /locus_tag="PKN53_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOD88537.1"
                     /translation="MSNHKFHIKKGDTVVVITGESKGKQGRVLEVLRSKERAIVEGVN
                     LISKHQKPSAKHPQGGIIKKEASVHISNLMLVEPKTGKPTRIGRKRNDDGKLIRISKK
                     TGEEIK"
     gene            3056..3619
                     /gene="rplE"
                     /locus_tag="PKN53_06015"
     CDS             3056..3619
                     /gene="rplE"
                     /locus_tag="PKN53_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOD88538.1"
                     /translation="MEKKFVPTLKKQYQEQIVNSLMKEFNYKTVMQVPRLEKIVINQG
                     LGQAISDKKLIDIAIEEMTTIAGQKAVATVSKKDISNFKLRKKMPIGVRVTLRRDRMY
                     EFLERLLAVALPRIRDFRGINYKFDGKGNYTLGISEQIIFPEIDIDKINRILGMDITF
                     VTTAKTDEEGYALLREFGFPFKNLKKN"
     gene            3621..3890
                     /gene="rpsN"
                     /locus_tag="PKN53_06020"
     CDS             3621..3890
                     /gene="rpsN"
                     /locus_tag="PKN53_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOD88539.1"
                     /translation="MAKESMKAREVKRLKLVKKYEAKRKQYKEEGNLEALHLLPRNSS
                     RVRLHNRCSQGGRPKGYMRQFGVSRIVFREMASNGLIPGVKKASW"
     gene            3912..4307
                     /gene="rpsH"
                     /locus_tag="PKN53_06025"
     CDS             3912..4307
                     /gene="rpsH"
                     /locus_tag="PKN53_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOD88540.1"
                     /translation="MTDPIADYLTRIRNAIMAGHKIVEIPGSNIKREMTKVLFDKGYI
                     LNYKFEDIGYQGNIKIALKYHPVTKFSAIKKLERVSKPGLRKYVNAENLPRVLNGLGI
                     AILSTNKGVMTDKEARRENIGGEVLCYVY"
     gene            4319..4873
                     /gene="rplF"
                     /locus_tag="PKN53_06030"
     CDS             4319..4873
                     /gene="rplF"
                     /locus_tag="PKN53_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOD88541.1"
                     /translation="MSRIGKLPITIPANVTVNVSDTNLVTVKGALGELKQQVDPAIKL
                     TIDGNVLTVSRPNDEKENRSKHGLYRALIANMVKGVSEGFTTEQEFIGVGFKAELNGQ
                     VLDMSLGFSHSVLLELPKEIQAECISERRGNTILKMKCADKQLLGQVAAKIRSLRPPE
                     PYKGKGIKFVNEELRRKAGKSAKV"
     gene            4893..5252
                     /gene="rplR"
                     /locus_tag="PKN53_06035"
     CDS             4893..5252
                     /gene="rplR"
                     /locus_tag="PKN53_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOD88542.1"
                     /translation="MALSYKIERRLRIKRSIRSKVFGTAEKPRMSVFRSNKQISVQVI
                     DDVSARTLVAASSKHKDIAETKATKSQKAAMVGKLIAKLALEAGITQVVFDRNGYLYH
                     GKVKALADGAREGGLKL"
     gene            5258..5776
                     /gene="rpsE"
                     /locus_tag="PKN53_06040"
     CDS             5258..5776
                     /gene="rpsE"
                     /locus_tag="PKN53_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOD88543.1"
                     /translation="MLRNIRKVKTTSDLELKDRLVSIQRVTKVTKGGRTFSFSAIVVV
                     GNENGVVGYGLGKASEVTTAIGKAVEEAKKNLVKVPVLKGTIPHEQAAKFGGAKVWLK
                     PATSGSGVKAGGAMRAVLESAGITDILAKSKGSSNPHNLVKATFAALIELRDANDVAY
                     QRQVSLDKVFNG"
     gene            5787..5966
                     /gene="rpmD"
                     /locus_tag="PKN53_06045"
     CDS             5787..5966
                     /gene="rpmD"
                     /locus_tag="PKN53_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOD88544.1"
                     /translation="MAKIRITQIKSKIGSTERQKQTLEALGIRKLNHSVEHEANPQIL
                     GMVTKVRHLVKVEEI"
     gene            5988..6434
                     /gene="rplO"
                     /locus_tag="PKN53_06050"
     CDS             5988..6434
                     /gene="rplO"
                     /locus_tag="PKN53_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOD88545.1"
                     /translation="MDLSSLTPAEGSIKKRKRIGRGQGSGKGGTSTRGHKGAKSRSGY
                     KRKIGFEGGQMPLQRTSPKFGFTNRNRIEYKGINVSILQTLAETKKLTEISVDTLVEN
                     GLVSKKDLIKILGNGELSVKLTVKAHAFSKTAIEKIEALGGNIEKI"
     gene            6438..7769
                     /gene="secY"
                     /locus_tag="PKN53_06055"
     CDS             6438..7769
                     /gene="secY"
                     /locus_tag="PKN53_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOD88546.1"
                     /translation="MKRFIETIKNIFKIEDLRSRIFITLGYILIYRLGAHIVLPGIDP
                     NQLAELQSQTKEGVLSLLDMFSGGAFSKASIFALGIMPYISASIVVQLLGIAVPYFQR
                     LQKEGESGRKKINQITRYLTIGILIIQSPSYIANLHHTLPSSAFVLQGNFFYISTVLI
                     LTAGTMFVMWLGEKITDRGLGNGISLIIMVGIIARLPFAFTAEITTQYKAAGGLLFVL
                     VELIILFVIFMVSIALVQAVRRIPVQYAKRVVGNKQYGGVRQYIPLKVNAAGVMPIIF
                     AQALMFVPLMIAGFGGENMGGFATAFSNYTGFWYNFTFALLIIVFTYFYTAVTINPQQ
                     MAEDMKKNGGFIPGIKPGKKTVEFLDNIMSKITLPGSIFLAFIAIMPALAQLAGVNAQ
                     FAQFFGGTSLLILVGVILDTLQQIESHLLMRHYDGLTKSGRIKGRGSYSTI"
     gene            7785..8567
                     /gene="map"
                     /locus_tag="PKN53_06060"
     CDS             7785..8567
                     /gene="map"
                     /locus_tag="PKN53_06060"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOD88547.1"
                     /translation="MIYRKSEEEIELMRKSNLLVSRTLAMLANEICEGVSTLFLDKLA
                     EEFIRVNGGVPGFLNYNGFPNSLCISVNDVVVHGIPSGYMLKHGDIVSVDCGVVMNGF
                     NGDSCYTFEVGEVSPEVKALLVCTKECLYKGIEQAISGNSIGDIGYAIQKHAESNGYS
                     VVRELVGHGIGKKLHEDPEVPNYGKPGRGVKLVDRMTIAIEPMINLGKQHIYQEYDGW
                     TIRTKDGKPSAHFEHTVVVRDGKADILSDFGMIEEVLKSKKS"
     gene            8571..8789
                     /gene="infA"
                     /locus_tag="PKN53_06065"
     CDS             8571..8789
                     /gene="infA"
                     /locus_tag="PKN53_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOD88548.1"
                     /translation="MAKQASIELDGTIIEALSNAMFRVELENGHVIIAHISGKMRMHY
                     IRILPGDKVKLEMSPYDLTKGRITYRYK"
     gene            8804..8920
                     /gene="rpmJ"
                     /locus_tag="PKN53_06070"
     CDS             8804..8920
                     /gene="rpmJ"
                     /locus_tag="PKN53_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOD88549.1"
                     /translation="MKVRASVKKRSDDCIIVKRKGRVYVINKKNPRYNQRQG"
     gene            8954..9319
                     /gene="rpsM"
                     /locus_tag="PKN53_06075"
     CDS             8954..9319
                     /gene="rpsM"
                     /locus_tag="PKN53_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOD88550.1"
                     /translation="MARIAGVDIPDNKRGEVALTYIYGIGRSRANKILGEAGVDRNIK
                     AKDWNDDQMAAIREAVGQFRLEGELRSEVQLNIKRLQDIGCYRGIRHRIGLPLRGQQT
                     KNNARTRRGKKKTIANKKK"
     gene            9327..9716
                     /gene="rpsK"
                     /locus_tag="PKN53_06080"
     CDS             9327..9716
                     /gene="rpsK"
                     /locus_tag="PKN53_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOD88551.1"
                     /translation="MAKKTKVTKKRVVKVESTGQAHIHTSFNNIIVTLTNNEGQVISW
                     SSSGKNNFRGSKKNTPYAGKMAAEDCGKVAFDLGLRKVKAFVKGPGAGRESAIRTLHN
                     LGIEVTEIVDVTPLPHNGCRPPKRRRV"
     gene            9741..10349
                     /gene="rpsD"
                     /locus_tag="PKN53_06085"
     CDS             9741..10349
                     /gene="rpsD"
                     /locus_tag="PKN53_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOD88552.1"
                     /translation="MARYTGPRSKIARKFGEPIYGPDKVLEKKNYPPGIHGLNKKRKK
                     TSEYGIQLREKQKVKYTYGLLERQFRNTFKKADSKKGVTGVVLLQLLESRLDNVVFRL
                     GLAPTRAAARQLVSHRHIVVNDKVCNIPSYQIKPGDIVGVRERSKSLEVVNNALSGFN
                     HSKYPWLEWDADAMAGKFMSIPEREDIPENIKEQLIVELYSK"
     gene            10362..11354
                     /locus_tag="PKN53_06090"
     CDS             10362..11354
                     /locus_tag="PKN53_06090"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOD88553.1"
                     /translation="MAILAFQKPDKVVMLEATDFHGKFEFRPLEPGYGITVGNALRRI
                     LLSSLEGFAISSVKIDGVDHEFSTIPGVMEDVTEIVLQLKQIRFKQQIEDMNHELVTV
                     VISGQEEFRAGDIGKFLSGFKILNPDLLICRMNSNVNLRLELNIVKGRGYVSPEDNKS
                     IDDLIGVIPIGSVFTPIRNVKYYIENMRVEQKTDYEKLLIEIDTDGSIHPKDALKEAA
                     KILIQHFMLFSDEKITLDDDDRLANDEFDEEVLRMRQLLKTKLVDLDLSVRALNCLKA
                     AEIDSLGDLVQFNRTDLLKFRNFGKKSLTELENLLEMKNLNFGMDISKYKLDKE"
     gene            11366..11968
                     /gene="rplQ"
                     /locus_tag="PKN53_06095"
     CDS             11366..11968
                     /gene="rplQ"
                     /locus_tag="PKN53_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOD88554.1"
                     /translation="MRHNKKFNHLGRTSAHRQAMLSNMAASLIVHKRIRTTVAKAKAL
                     RVYIEPLLTKSKEDTTHNRRVVFSYLENKEAVAELFRDVAVKIADRPGGYTRILRTGT
                     RLGDNADMCIIELVDYNEAMLEAKETKKKTSRRSRGRKSGKAKTEEQVQEVEEVVSNT
                     ENALEETAVETTEEPVAEEPVAEVEESVEEEKDQDAEEKK"
     gene            12056..13345
                     /gene="eno"
                     /locus_tag="PKN53_06100"
     CDS             12056..13345
                     /gene="eno"
                     /locus_tag="PKN53_06100"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOD88555.1"
                     /translation="MGQIANIHARQILDSRGNPTVEVEVYTTQGVVGRAAVPSGASTG
                     AYEAVELRDNDKTKYLGKGVLQAVHNVNSVIDENLKGFYVEDQLSIDSALIKLDGTAN
                     KSNLGANAMLAVSMACAQAASTEHGLSLYNYLGGVNARVLPIPLMNILNGGQHADNLI
                     DIQEFMIMPVSAPTFSEALRMGTEIFHNLKAVLKSGGYATNVGDEGGFAPNLKSNEEA
                     IEYVLKGIERAGYKPGEDVFIALDAAASEFYHKDKNMYIFESNGSKMTSEQMVEYWVE
                     WTKKYPVISIEDGLDQDDWTGWKQMTDKLGDKIQIVGDDLFVTNPARLAKGIKEKTAN
                     SILIKLNQIGTVSETIEAVNLAHNNSYTAVISHRSGETEDVFIAELAVALNTGLIKTG
                     SASRSDRIAKYNQLLRIEEVLGANAMYLGKNFKFLKK"
     gene            13484..13762
                     /locus_tag="PKN53_06105"
     CDS             13484..13762
                     /locus_tag="PKN53_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOD88556.1"
                     /translation="MTKIGRPMAGKVLIQPLAAEQKTSSGIIIPDSAQEKPLKGKIIA
                     VGSAKEGEKMEVKVGDMVLFGKYSGTEIKVDDVDYLIMNQSDILLVIE"
     gene            13795..15432
                     /gene="groL"
                     /locus_tag="PKN53_06110"
     CDS             13795..15432
                     /gene="groL"
                     /locus_tag="PKN53_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941147.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOD88557.1"
                     /translation="MAKEIKFEIEARDLLKKGVDSLADAVKVTLGPKGRNVVIEKKFG
                     APQITKDGVTVAKEIELKNPFMNMGAQMVKEVASKTNDDAGDGTTTATVLAQSIVGVG
                     LKNVTAGANPMDLKRGIDKAVSAVVDEIKKMSESIGNDISKIEQIAMVSANNDSAIGK
                     DIAKAMEKVKKEGVITIEAAKGTDTEVKVVEGMQFDRGYISPYFITDGEKMEAVLESP
                     YILITDKKISSMKDLLPVLEPVAQEGGQLLIIAEDVEGEALATLVVNRLRGTLKIAAV
                     KAPGFGDRRKEMLQDIAILTGGEVITEERGLKLENANISQLGKAEKVVIDKENTTIVD
                     GVGNKEQIQARVAQIKKQIEITTSEYDKEKLQERLAKLAGGVAVIYVGATTEVEMKEK
                     KDRYEDALSATRAAVEEGIIPGGGVAYIRAISALNKIKVDNEDEKLGVSIVLRALEEP
                     LRRIVENAGKEGSVIVQKVKEGKGDYGYNARTDVFENLYESGVIDPAKVARVALENAA
                     SIAGMLLTTECVIAEEKEEHPEMQMPNQGMGGMGGMM"
     gene            15656..15910
                     /gene="rpsT"
                     /locus_tag="PKN53_06115"
     CDS             15656..15910
                     /gene="rpsT"
                     /locus_tag="PKN53_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOD88558.1"
                     /translation="MAHHQSAKKRIRKSEARRVHNKYYARTTRNAVRDLRASTNKAEA
                     EEMLPKVTALLDKLAKKNIIHSNKAANIKSSIAKHVNSLA"
     gene            16016..16705
                     /gene="radC"
                     /locus_tag="PKN53_06120"
     CDS             16016..16705
                     /gene="radC"
                     /locus_tag="PKN53_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOD88559.1"
                     /translation="MRNKKSIKYWADDDKPREKILQKGSQALSDVELLAILLGSGSAN
                     QSAIELSRSILNDVSNNLNLLGKITAKDLMKYSGVGLAKASTILAALELGRRRKQEEV
                     LQQKIIKSSQDVYDYFQPILGDLSHEEFHVLFLNRANAVISSKLISSGGTTGTFIDIK
                     LVMKNALENLAQSVIIAHNHPSGNAVPSGNDIEVTKKIKNACEFFDINFFDHIIIADK
                     SFYSFADKGLI"
     gene            16720..19731
                     /locus_tag="PKN53_06125"
     CDS             16720..19731
                     /locus_tag="PKN53_06125"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOD88560.1"
                     /translation="MKRIIVPTVLTIAVSFILVYLLFFYESPKKEFIVGEYSELVNPF
                     IGTDYHGHTHPSAMLPFGMVQLGPETRLEGWDGCSGFHYSDSIIYGFTHTHLSGTGVA
                     DYGDILLMPFSNFDNDFSKDKIASKFRYDNTVASPGYFSTYLEDNGVNVYLTVTERVG
                     FHKYVYDNPQEKYILLDLEHRDEVLESYVKPISKTRFVGLRRSKSWAENQILYFVMDF
                     SQPVSIVDFESDVIVNDELIDNKTFKLNSANQTPSIYVGKNNSLVFKVDSNSENELLV
                     KVALSATGEEGAMKNLEAEIADWDFEKVKTNAKNIWDKELSKIRIEGGKEDDRIAFYT
                     ALYHAYSAPNIYCDVDGKYRARDLENYENPQHTTYTVFSLWDTYRAAHPLYTIVQEKR
                     TLDFIKTFLDQYKKGGALPMWELSANETMCMIGYHSVPVIVDAYFKGIKDFDLELALE
                     AMISVAKTDELGKAEFAKYGYIPLDMEHESVSKALEYAFDDWCIAMFAKDLGKQDVYE
                     EFIERAQFYKNVFNPKNGFMQSKLNAAWQEPFDPKEVNFNFTEANSWQYSFYVPQDIM
                     GFIELHGGNEKFAKKLDEMFSEESQTSGNHQVDITGLIGQYAHGNEPSHHMAYLYNYC
                     GKPWKTQAMARKIMSELYSAQPDGLCGNEDCGQMSAWYVLSAMGFYPVNPANGVYDLG
                     SPLFDKATIQLENGKHFEIIAHDNSEKNIYVKEVKLNGRKYKKSYICHADIMKGGKLE
                     FFMASEPETNRATEAKDIYQSQITENIITPVPFTNVPFRSFRDEVELTLDCADKNAEI
                     YYSINDEENANPQKYEKPIVINSTTKLKAYAKSDSKKQSKTIEAELIFIPDNRTIKLI
                     SEYSPQYTAGGDNALIDFIKGEAWFRSGLWQGYRGQDFEAIVDLQEVKEINLINARFL
                     QDIRAWIFFPKKVSYFASNDGVNFTKIYESDSQMPDNDYEVKIQEFKNERENFKARYI
                     KVVAKSYGKLPEWHLSAGYNSWIFIDEIEIY"
     gene            19731..20513
                     /locus_tag="PKN53_06130"
     CDS             19731..20513
                     /locus_tag="PKN53_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88561.1"
                     /translation="MKRILFLVICISVFSLISCENYKAKKKIKADLSDVQFEDKDLNY
                     DSIFALVPDYNEMLGILKDVRVGYSSKILLDSKSAEMFLTSKEVSFALGMFVADLAYV
                     RYFEKVQLCTEYLESLKILTEQLAISNKNFESLVKKVETNIQDRDELFLVVDSLLGEA
                     NKIFTKSEQHSLSCLALSGFWLEISYLGLNSINAENIENNSDFFHSHFEILKLINQLY
                     SCLEDESFINPLKQNFLELEAKGLENLELEEDILKIRSDYVK"
BASE COUNT         7837 a         2841 c         4115 g         5907 t
ORIGIN      
        1 aaaaaggtcc attttttgac tttaatttat ggagaaaagt aattgcaatg gatgcaagcg
       61 gaaagaaaaa tgccattaaa acatgggcaa gagcttccgt aattccacca gaatttgtgg
      121 ggcatacttt tgcagttcat aatggaaata aatttattcc tgtttatgtt actgaaaata
      181 tggtgggtca cagattgggc gagtttgcac cgacaagaac atatagaggt catggtggta
      241 aaaagaaata aaataaatta gtattatggg cgcacgtaaa agaataaaag cagaacaaag
      301 aaaagaagca aaaaagcata ttagctatgc tgttcttcgc aattgtccga gttcacctcg
      361 taaaatgaga ctcgtggcag atcttgttag aggccaaaaa gtagatagag ctttagatat
      421 cttacatttt tcaccaaaag ctgccgcacg cgatgttgaa aaacttttaa aatctgcagt
      481 ttctaactgg caagctaaga acgaaggaca aagaatagaa gatgtagaac tttatgttac
      541 agaaataact gttgatgaag cacgtccttt aaaacgaata aaacccgctc ctcaaggaag
      601 ggcttatcgt attagaaaac gttcaaacca tgttagaatt gtggttgata caaagagaga
      661 aaaagaatta ttaaatgaag aaaatgcata aaattcatgg gacaaaaatg taatcctata
      721 agcaacagat tgggtatcat cagaggatgg gactctaatt ggtatggcgg aagaaaatat
      781 gccgacaaat tagctgaaga tgctaaaatt cgtcgctatt taaacgcacg tttagaaaag
      841 gcaagcgttt caaagattat tatagaaaga tctttgaaaa tgataactat tactataaac
      901 actgcacgtc ctggttttgt aatcggaaaa ggtggagacg aagtagataa acttcgcgaa
      961 gagttgaaga aaattacaaa taaagatatt caaataaata tctttgaaat taaacgccca
     1021 gaattagaag cagttttagt tggtaataat gttgcaagac aaatcgaagg taaaattggt
     1081 tatcgtcgtg caattaaaat ggctattgct ttaacaatgc gtatgggtgc cgaaggaatt
     1141 aaaattcagt gctcaggacg tttgaatggt gcagaaatgg caagaagcga aatgtataaa
     1201 gaaggtagaa cacctttaca tacattaaga gcagatattg actatgcttt gtcagaagct
     1261 ctaacaaaaa caggtttgtt aggtataaaa gtttggatat gcagaggtga gatttatggt
     1321 aaaagagatt taagtccaaa cataggaaaa tctgttcaag gtaaaagacc agcagcagca
     1381 tctggacgag aacgtggtag tagaagaaga aagaaataat ttgaaaacta agagctatgt
     1441 tacagccaaa aaagtcaaaa tttagaagac agcaaaaagg aagaatgaaa ggcaatgccc
     1501 aacgtggaaa ccaaatcgct tttggttcgt ttggcattaa agctttagag tcggcttgga
     1561 taacaggtcg tcaaatcgaa gctgctcgta tcgctgttac aagaaaaatg caacgtcaag
     1621 gacaaatctg gattcgtata tttcctgata aaccagttac aagcaaacct gcagaggttc
     1681 gtatgggaaa aggaaaagga gctccagaag gctttgtcgc aagagtaaca cctggacgaa
     1741 tacttttcga agcagaaggt gtaccatttg atttagcaca agaagctttg agactagcag
     1801 ctcaaaaact tccaattaaa acgaaattca tcgttagaca tgattatgat tataataaat
     1861 aaatcagaga gagatgaaga ataaagaaat aagaggttta tcaaccaaag aaatcgccga
     1921 cagattagta agcgagcagc ataacttaac taagttaaga ataaatcatg cagtatcgcc
     1981 tttagacaat cctcagcgaa ttaaagaggt aagaagatta attgcaagac tcaaaacaga
     2041 gttgcgtaac agagaattaa cagaaataaa ataagataca gctatggaaa ctagaaattt
     2101 aagaaaagag cgtatcggta tcgttgtaag caacaaaatg gataaatcaa ttgttgttgc
     2161 cgtaaaaagg aaagaaaaac atcctaaata cggtaagttt gtgaataaaa caaaaaagtt
     2221 tgttgctcac gatgaaacta acacttgcga tataggcgat actgttcaaa taatggaaac
     2281 tcgtcctttg agcaaaacta agagatggag acttataaat ataattgaaa aagttaaata
     2341 gtcatgatac aacaagaaag cagattgaat gtagcagaca atagtggcgc aaaagaagta
     2401 ttgtgtattc gcgttcttgg cggaacaaaa cgcaaatatg cttctatagg cgacaaaatt
     2461 gttgtaagcg taaaaagtgc attgccaggt ggtgatgtga aaaaaggcgc agttgctaaa
     2521 gcagttatag tgagaaccaa aaaagaagtt agacgccaag atggatcgta tataagattt
     2581 gacgataatg cttgtgtgtt gttaacagca gctggcgaac taagaggaac tcgtattttt
     2641 ggtcctgtag ctagagaatt gcgtgaaaaa caatatatga aaattgtatc attggcgcca
     2701 gaagtattat aaaaattaaa agaaaagaca tgagtaatca taaatttcat atcaaaaaag
     2761 gcgacacagt agtcgttatt actggggagt ctaagggtaa gcaaggaaga gttcttgagg
     2821 ttttgagatc taaagaaaga gctattgtag aaggcgtaaa tcttatctct aaacatcaaa
     2881 aacctagcgc aaaacaccca caaggtggta ttattaagaa agaagctagc gttcacattt
     2941 ctaacctaat gcttgttgaa ccaaaaactg gaaaaccaac acgcattgga cgtaaaagaa
     3001 atgacgatgg aaaattaatc agaatttcta aaaaaactgg agaggagatt aaataatgga
     3061 aaagaaattt gtacctacac ttaaaaaaca gtatcaagag caaatagtta actctttaat
     3121 gaaagaattt aactataaaa cagtaatgca agtgcctcgc cttgaaaaaa tagttataaa
     3181 tcaaggtctt ggacaagcta tttctgataa aaaattaatt gatattgcta ttgaagaaat
     3241 gaccacaata gcaggtcaaa aagctgtagc aacagtttct aaaaaagata tttctaactt
     3301 taaattacgt aaaaaaatgc ctattggtgt aagagttact ttgcgtagag atagaatgta
     3361 tgagttttta gaaagattgt tggcagtagc tttacctcgt attagagatt ttcgtggtat
     3421 caattacaaa ttcgacggaa aaggaaatta tactttaggt atatcagaac aaattatttt
     3481 cccagaaatt gatattgata aaataaaccg catattagga atggatatca cttttgttac
     3541 tacagcaaaa acagacgaag aaggatatgc attacttaga gaatttggat tcccatttaa
     3601 aaatttaaaa aagaattagt atggcaaaag aatcaatgaa agctcgcgaa gttaagagat
     3661 tgaagctagt aaaaaaatat gaagctaaaa gaaaacagta taaggaagaa ggcaatttag
     3721 aagctctaca ccttttacca agaaattcat ctagagttag attacacaat cgttgtagtc
     3781 aaggtggaag accaaaaggt tatatgagac agtttggcgt aagccgtatt gttttccgtg
     3841 aaatggcttc aaatgggtta atcccaggtg ttaaaaaagc aagttggtaa taattttaaa
     3901 aattttgaat aatgactgat cctattgcag attatttaac cagaatcaga aatgctatca
     3961 tggcaggaca taaaattgtt gaaatccctg gttcaaacat taagcgcgaa atgacaaaag
     4021 tgctttttga taaaggttat attttgaatt ataagtttga ggatatcggt tatcaaggaa
     4081 atattaagat tgctcttaaa tatcatcccg taaccaaatt ctcagctata aaaaaattag
     4141 agagagtaag taaacctggt ttgagaaaat atgtaaatgc cgaaaaccta cctcgagtat
     4201 taaatggttt aggtattgca attttatcaa caaataaagg tgtgatgacc gacaaagaag
     4261 ctcgtagaga aaatattggc ggtgaagtat tgtgttacgt ttattaaaaa attgaaaaat
     4321 gtcaagaatt ggtaaattac ccataactat acctgcaaat gttacagtaa atgtttcaga
     4381 cacaaatctt gtaacagtaa aaggagcttt gggagaactt aaacagcagg tcgatccagc
     4441 tataaaatta acaattgacg gaaacgtttt aactgtaagc agaccaaatg acgaaaaaga
     4501 aaatagatca aaacatggtt tgtatagagc tttaatagca aatatggtta aaggagtttc
     4561 tgaaggtttt actaccgaac aagaatttat tggcgttgga tttaaagccg aactcaatgg
     4621 acaagtattg gatatgtctt taggcttttc tcatagcgtt ttgcttgaat taccaaaaga
     4681 aatccaagca gaatgtataa gcgaaagacg tggaaacact attttaaaaa tgaaatgtgc
     4741 agataaacaa ttgcttggac aagttgcagc aaaaattcgt tcattaagac caccagagcc
     4801 ttataaagga aaaggtatta aatttgttaa cgaagaatta cgtagaaaag caggtaaatc
     4861 tgctaaagtc taatttgtaa aaatttaaaa agatggcttt atcttataaa atagaaagac
     4921 gattaagaat aaaacgcagt atccgcagta aagtgtttgg tactgccgaa aaacctagaa
     4981 tgtctgtatt tcgtagcaat aaacaaataa gcgttcaggt aatagatgat gttagtgcta
     5041 gaacattagt agcagcaagc tctaaacata aagatattgc tgaaactaaa gcaacaaaat
     5101 ctcaaaaagc agcaatggtt gggaaactaa ttgcaaaact tgctttagaa gctggcatca
     5161 cacaagtggt ttttgataga aatggttact tgtatcatgg taaagtgaaa gctttggctg
     5221 atggtgcaag agaaggtgga cttaaactat aataactatg ttaaggaata ttagaaaagt
     5281 aaaaacaact agcgatctgg aattaaaaga cagacttgtt agtatccaaa gagtaacaaa
     5341 agttacaaaa ggtggtagaa catttagttt ttctgctatt gtggtagtgg gaaacgaaaa
     5401 cggcgtagta ggatacggtt taggaaaagc cagtgaggtt actactgcta taggaaaagc
     5461 agttgaagaa gctaagaaaa acttagtgaa agtaccagta ctaaaaggaa caattcctca
     5521 tgagcaagct gctaaatttg gcggagctaa agtatggtta aaacccgcaa cttcaggttc
     5581 tggagttaaa gccggtggtg cgatgcgtgc agttttagaa agtgctggta taactgatat
     5641 tttggcaaaa tctaaaggct cttcaaatcc tcataacctt gtcaaagcta cttttgcagc
     5701 acttatagaa ttaagagatg caaacgatgt cgcctatcaa cgacaagtga gtctcgataa
     5761 agtatttaat ggataaaaat ttagttatgg ctaaaattag aattactcaa ataaaaagta
     5821 aaatcggtag tactgagcgt caaaaacaaa ctctagaagc tttgggtatt agaaaattaa
     5881 atcactcagt tgagcatgaa gcaaacccac agattttagg aatggtaacg aaagtacgcc
     5941 acttagtaaa agttgaagaa atataataat ttaaaaatat ttatattatg gatttaagta
     6001 gtttaacacc agcagaaggt tctatcaaga aaagaaaaag aataggtaga ggtcaaggtt
     6061 caggaaaagg cggaacttct acaagaggac ataaaggtgc aaaatcaaga tccggctata
     6121 aacgtaaaat aggttttgaa ggaggacaaa tgcctttgca aagaacttct ccaaaatttg
     6181 gatttacaaa tcgtaatcgt atcgaatata aaggtatcaa tgtttctatt ttacaaactt
     6241 tggcagaaac caaaaaactt accgaaatat ctgttgatac actagttgaa aatggattgg
     6301 tttctaaaaa agatttaatt aaaattttag gaaatggcga actttctgtt aaattaacag
     6361 ttaaagcaca cgctttttct aaaacagcaa tagagaaaat agaagcatta ggtggaaata
     6421 ttgaaaaaat ttaataaatg aaaaggttta ttgaaacaat aaaaaacatt tttaagattg
     6481 aggatctacg ttcaaggatt ttcataaccc ttggatatat acttatttat aggttaggtg
     6541 ctcatatcgt actaccggga attgacccaa atcaattagc agaattgcaa agtcaaacaa
     6601 aagaaggtgt attaagcctc ttggatatgt tttccggtgg tgcattttct aaagcatcaa
     6661 tttttgcttt ggggattatg ccatatatct cggctagtat cgttgtgcaa ttattaggaa
     6721 ttgctgttcc ttattttcaa cgactacaaa aagagggaga aagtggtagg aaaaagatta
     6781 atcaaataac acgctatttg actataggta ttcttataat tcaatcgcct agttatattg
     6841 ctaacctaca ccacaccttg cctagctcgg catttgtact tcaaggaaat tttttctata
     6901 tttccacagt gctaattttg acagcaggca ccatgtttgt tatgtggttg ggtgagaaga
     6961 ttaccgacag aggtttagga aatggaatat cattgataat tatggtcggt attatagcaa
     7021 gattgccatt tgcatttact gctgagataa caacacagta taaagcagct ggtggtttgt
     7081 tgtttgtatt agttgagtta ataatattgt tcgtgatttt catggtatca atagcgctcg
     7141 ttcaagctgt tagaagaata cctgttcaat atgctaaaag agtagttgga aataaacaat
     7201 atggtggtgt gagacaatac atacctctaa aagtaaatgc tgctggagta atgccaatta
     7261 tctttgctca agctttaatg ttcgtgcctt tgatgattgc aggatttggt ggtgaaaata
     7321 tgggtggctt tgctactgcg ttttcaaact atactggttt ttggtataat ttcacatttg
     7381 cattactgat tatagtcttc acatatttct atacagcagt aacaataaat cctcagcaaa
     7441 tggctgaaga tatgaagaaa aacggtggat ttatccctgg aattaaacca ggtaaaaaaa
     7501 ctgttgaatt tttagacaat attatgtcga aaattacact tccaggatct attttcttgg
     7561 cgtttattgc aattatgcca gctttggctc aacttgcagg tgttaacgct cagtttgcac
     7621 agttctttgg aggtacttca cttttgattc ttgtaggggt aatattagat acactacaac
     7681 aaatcgaaag tcacttatta atgcgtcact atgacggatt aacaaaaagt ggtagaataa
     7741 aaggtagagg ctcgtactct acaatttaaa agaaaggttc tttgatgatt tatagaaaga
     7801 gcgaggaaga aattgaattg atgagaaaaa gcaatttgct tgtgtcaaga acacttgcaa
     7861 tgcttgcaaa cgagatttgt gaaggagttt caacattatt cctcgacaaa ttagcagaag
     7921 agtttatcag agttaatggc ggagttccag gttttttaaa ctataatggt ttcccaaata
     7981 gtttatgtat ctcggttaac gatgtcgtag ttcatggtat tccttcgggc tatatgttga
     8041 aacatggaga tatagtgtcg gttgattgtg gagtagtaat gaatggtttc aacggagact
     8101 catgctatac ttttgaagtg ggagaagtaa gtcctgaagt aaaggctttg ttggtgtgta
     8161 caaaagaatg tctttacaaa ggaatagagc aagcaattag cggaaacagt atcggcgata
     8221 ttggatacgc tatccaaaag catgcagagt ctaatggata ttctgtcgtt agggagttgg
     8281 tagggcacgg aatagggaaa aaattacacg aagatccaga agttcctaat tatggaaaac
     8341 ctggaagagg tgttaagtta gttgacagaa tgaccatagc aattgagcca atgattaatc
     8401 ttgggaaaca acatatttac caagaatatg atggttggac aattagaaca aaagacggaa
     8461 aaccgtcagc tcattttgag catacagtgg tagttagaga tggaaaagca gatatattat
     8521 ctgattttgg aatgattgaa gaagttttaa aaagtaaaaa aagttagttt atggcaaaac
     8581 aagcttctat agaattggac ggaacaatta ttgaagcatt atctaatgca atgtttcgtg
     8641 ttgaacttga aaatggacat gttatcatcg cccatatttc aggtaaaatg cgtatgcatt
     8701 atatacgtat tttgccgggc gataaagtta aattggaaat gtcgccttac gatctaacaa
     8761 aaggaagaat aacatacagg tacaaataaa aaatattaaa acaatgaaag taagagcatc
     8821 tgtaaaaaag agatcagacg attgtataat cgttaaaaga aaaggtagag tttacgtgat
     8881 aaataagaaa aaccctagat ataaccaacg tcaagggtaa taattaatta ataatttaaa
     8941 caaatttacg aatatggcaa gaatagcagg tgtagatata ccagacaaca agcgaggaga
     9001 agtcgcgctt acctacatct atggaattgg tcgcagcaga gcaaataaaa ttttaggcga
     9061 agctggtgtt gaccgaaaca taaaggcaaa agattggaac gatgaccaaa tggcagcaat
     9121 tcgtgaggct gtaggtcagt ttcgtcttga aggtgaactt agaagtgaag ttcaacttaa
     9181 cattaaacgc ttgcaagata ttggttgcta tcgcggtatc cgtcatcgta tcggtttacc
     9241 attaagagga cagcaaacaa aaaataatgc aagaactcgt agaggtaaaa agaaaactat
     9301 tgccaataaa aagaaataat tgagatatgg ctaagaaaac taaagttaca aaaaaacgtg
     9361 tagttaaagt agagtcaacc ggacaggcac acatacatac gtcttttaat aacattattg
     9421 ttactttaac aaataatgaa ggacaagtta tttcatggtc atcttccgga aaaaataatt
     9481 ttagaggctc taaaaagaac acaccttatg caggtaaaat ggcagcagaa gattgtggaa
     9541 aagttgcttt tgatttagga ttacgcaaag ttaaagcttt cgtaaaagga ccaggtgcag
     9601 gacgtgaatc tgctataaga actttacaca atttaggaat tgaagttacc gaaatagttg
     9661 acgtaactcc tttaccacac aatggttgca gaccacctaa aagaagaaga gtttaataat
     9721 aaattaataa gaataaggaa atggcaagat atacaggacc gcgatcaaaa attgcaagaa
     9781 aattcggaga accaatctac ggaccagata aagttttgga aaagaaaaac tatcctccag
     9841 gaatacatgg tcttaacaaa aaaaggaaaa aaacttctga atatggtatc cagttgagag
     9901 aaaagcaaaa agtaaaatat acttacgggc ttttagaaag acaattccgc aacacattta
     9961 aaaaagctga tagcaaaaaa ggtgttactg gtgttgtatt attacaactt ttagaatctc
    10021 gtttggataa tgttgttttt cgtttaggac ttgcaccaac aagagcagct gcacgccaat
    10081 tagtgtcaca tcgtcatatc gttgttaatg acaaggtttg caatataccg tcttatcaaa
    10141 taaaaccagg cgatatagtt ggagttagag aaagatctaa atctttagaa gtggttaaca
    10201 acgcattgag cggatttaat catagcaaat atccttggct agaatgggac gctgatgcaa
    10261 tggcaggtaa atttatgtct atcccagaaa gagaagacat accagaaaac attaaagaac
    10321 agttaatagt tgaattgtat tcaaaataaa aattaaagta aatggcaatt ttagcatttc
    10381 aaaaacccga taaagtagta atgctagagg caacagactt tcacggaaag tttgagtttc
    10441 gccctctaga accaggttat ggaattacag taggcaacgc attgagaaga atattactat
    10501 catcactcga aggatttgct atttcatcag taaaaattga tggagtagat cacgaattct
    10561 ctacaatacc tggagtgatg gaagacgtta ccgaaattgt gttgcaatta aaacaaatac
    10621 gttttaaaca acaaatagaa gatatgaacc acgagcttgt tacagttgta atttctggac
    10681 aagaagaatt tagagcagga gatattggca agtttttatc tggttttaaa attttaaatc
    10741 ccgatttgct aatttgtaga atgaactcta atgtaaatct aagattggaa ttaaatatag
    10801 ttaaaggacg tggttatgta tctccagaag ataataaatc aatagacgac cttattggag
    10861 ttattccaat cggttctgtg tttacaccta ttagaaatgt aaaatattac atagaaaata
    10921 tgcgtgtaga acaaaaaaca gactacgaaa aattattgat agaaattgat actgatggct
    10981 ctattcatcc taaagacgca ttaaaagaag ctgcaaaaat tttaatacag cactttatgt
    11041 tattctctga cgagaaaatt actttagatg atgacgatag attagctaac gacgagtttg
    11101 atgaagaagt tctaagaatg cgtcagttac taaaaactaa attagttgac ctcgaccttt
    11161 cagttagagc acttaattgc ttaaaagcag cagaaatcga cagtttagga gatttagtac
    11221 aatttaatag aactgatttg ttgaagttta gaaactttgg taaaaaatca cttaccgaac
    11281 ttgaaaatct tttagaaatg aaaaatttga actttggtat ggatatttca aaatataagt
    11341 tggacaagga ataatattat aagaaatgag acataataag aaatttaatc atttaggtag
    11401 aacgagtgca cacagacaag caatgttgtc taatatggca gcttcgctaa ttgtgcataa
    11461 acgtattaga actactgttg caaaagcaaa agcacttaga gtatatatag aacctcttct
    11521 tacaaaatca aaagaagata ctacacataa tagaagggtt gtatttagct atttagaaaa
    11581 taaagaagct gtagctgaat tgttccgcga tgtagctgtt aaaatagcag accgcccagg
    11641 tggttatact agaatattga gaacaggaac tcgtttagga gataacgctg atatgtgtat
    11701 aattgagttg gttgactaca acgaagctat gctcgaagct aaagaaacta aaaagaaaac
    11761 aagccgtcgt tcaagaggta gaaagtctgg taaggctaaa actgaagaac aagtacaaga
    11821 agttgaagaa gtagttagca ataccgaaaa tgcacttgaa gaaactgctg tagaaacaac
    11881 agaagaacct gttgcagaag aacctgttgc tgaagtagaa gaaagtgttg aggaagaaaa
    11941 agaccaagat gctgaagaaa aaaagtaaga ctaatgataa aaaaataata ttttaatagg
    12001 ggggtattgt atatcccctt ttttatgtta tatttgaaaa aaacttaatt ttattatggg
    12061 acaaatagca aatattcatg caagacaaat tttggattca agaggaaatc caacagtaga
    12121 agtagaagtt tatacaacac aaggagttgt tggtagagca gccgttccaa gtggagcgtc
    12181 aacaggagct tacgaggctg tagagttaag agataacgat aaaacaaaat atctcggtaa
    12241 aggagtgctt caagctgtac ataacgtcaa ttctgtaatt gatgaaaacc ttaaaggttt
    12301 ttatgttgaa gaccaattat ctatcgactc agctttgata aaactcgatg gaactgcaaa
    12361 taaatcaaac ttgggagcaa atgcaatgtt ggcagtttca atggcttgtg ctcaagcagc
    12421 atcaacagaa cacggattgt cattgtataa ctatctaggt ggagttaatg caagagtttt
    12481 accaattcca ttgatgaata ttctaaatgg aggtcaacat gcagataacc taattgatat
    12541 tcaggagttt atgattatgc cagtttctgc accaactttt agcgaagctc taagaatggg
    12601 aacagaaatt tttcataatc taaaagcagt tctaaaaagc ggaggctatg caactaacgt
    12661 aggtgatgaa ggtggatttg ctccaaacct taaatcaaac gaagaagcaa tagaatacgt
    12721 tctaaaagga attgaaagag ctggatacaa accaggcgaa gatgtgttta tcgctcttga
    12781 tgcagcagct tcagaatttt atcataaaga taaaaacatg tatatcttcg aatctaatgg
    12841 cagtaaaatg acatcagaac aaatggttga atattgggta gaatggacta aaaaatatcc
    12901 agtaatctct atcgaagacg gtctagacca agacgactgg acaggttgga aacaaatgac
    12961 agacaaatta ggagataaaa ttcaaatagt tggagacgac ctgttcgtta ctaatccagc
    13021 tcgtttagca aaaggaatta aagaaaaaac agcaaactct attttgataa aattaaatca
    13081 aataggtaca gttagcgaaa ctattgaagc agtaaatctt gctcacaata actcttatac
    13141 tgctgttata agccatcgtt ctggagaaac agaagacgta tttattgccg aattagcagt
    13201 tgctttaaat acaggtttaa taaaaacagg ttctgcatct agaagcgata gaattgcaaa
    13261 atataaccaa ttactaagaa ttgaagaagt attgggagct aatgcaatgt acttaggtaa
    13321 gaattttaaa ttcttaaaaa aatagacaat ttgtcagttt agaaaaacta aaatccgatt
    13381 tgtctgaatg ttttgtcatg tttttacaaa tggcatagtt ttggcaaatc ggattttgtg
    13441 ttttaatata ataattgttt aactaaaatt aaaaaaaaga gatatgacaa aaattggaag
    13501 accaatggcg ggaaaagtac ttattcaacc acttgccgca gaacaaaaaa catcaagtgg
    13561 aattattatc ccagattcag cacaagaaaa acctcttaaa ggtaaaatta ttgcggtagg
    13621 aagcgcaaaa gaaggtgaaa aaatggaagt taaggttggt gatatggttt tgtttggaaa
    13681 atacagcgga accgaaatta aagtcgatga tgttgactat ctaataatga accaatccga
    13741 cattttatta gttatcgaat aataattata aaatcttaaa attgtaaaaa tattatggca
    13801 aaagaaatta aatttgaaat agaagctaga gacctactta aaaaaggtgt tgactctttg
    13861 gcagacgcag taaaagttac tttaggacca aaaggcagaa atgttgttat tgagaaaaaa
    13921 tttggcgcac ctcaaattac aaaagacggt gttacagttg ctaaagaaat agaacttaaa
    13981 aatccattta tgaatatggg tgctcaaatg gtaaaagaag ttgcatcaaa aactaacgat
    14041 gatgcaggag acggaactac tacagcaaca gttttagctc aatctattgt tggcgttggt
    14101 cttaaaaacg ttaccgcagg tgctaaccca atggacttaa aacgtggtat cgacaaagct
    14161 gttagtgcag ttgtagatga aattaagaaa atgagcgaat ctattggaaa cgacattagc
    14221 aaaatagaac aaattgcaat ggtgtcggct aataacgatt ctgctattgg taaagatatt
    14281 gcaaaagcaa tggaaaaagt aaagaaagaa ggcgttatta caatcgaagc agcaaaagga
    14341 actgatacag aagtaaaagt tgttgaaggt atgcaattcg atagaggata tatttctcct
    14401 tatttcatta ctgatggcga aaaaatggaa gctgttttag aatctccata tattctaatt
    14461 accgataaaa aaatatcttc tatgaaagat ttgctaccag tattagaacc agtagcacaa
    14521 gaaggaggac aactgttaat tattgctgaa gacgttgaag gcgaggcatt agcaacttta
    14581 gttgtgaatc gtttacgtgg tacattaaaa atagcagcag ttaaagctcc tggctttggc
    14641 gatagaagaa aagaaatgct tcaagatatt gcaatcctta caggtggtga agtaattacc
    14701 gaagaaagag gactgaaatt agaaaatgca aatatttctc aacttggaaa agctgaaaaa
    14761 gttgtgattg ataaagaaaa cactacaata gttgatggcg ttggaaataa agaacaaatt
    14821 caagctagag tagctcaaat taaaaagcaa atcgaaatca caactagcga atatgataaa
    14881 gaaaaacttc aagaaagatt ggcaaaactt gctggtggag tagctgtaat ttatgtagga
    14941 gcaactacag aagttgaaat gaaagagaaa aaagatagat atgaagacgc tttatcagca
    15001 acacgtgcag cagttgaaga aggtattata ccaggtggtg gagtagcata tattagagct
    15061 atttctgctt taaataaaat aaaagttgac aacgaagacg aaaaacttgg cgtttctata
    15121 gtattgagag cattagaaga accacttcgc agaattgttg aaaatgcagg aaaagaaggt
    15181 tcggtaattg ttcaaaaagt aaaagaaggt aaaggcgatt acggatataa cgcaagaact
    15241 gatgtgttcg aaaatctata cgaatcagga gtgattgacc ctgctaaagt tgctagagta
    15301 gcactagaaa atgctgctag tattgcagga atgttgctaa caactgaatg cgtaatagcc
    15361 gaagaaaaag aagaacaccc agaaatgcaa atgccaaacc aaggaatggg aggcatgggc
    15421 ggaatgatgt aatcattgct aaataaaaat agtaggacct ttttggtttg ggtccgaagc
    15481 aataaaccaa tatataaaca agagactgac tcaagaagag gcagtctctt tttgtttaaa
    15541 aaataaaata attaaaaaat atttcccaaa taaacttgtt tgaataaaaa attattgtat
    15601 ctttgcagtc caattttggg aagtaaataa tttaatatta gaaaataaag atattatggc
    15661 acatcatcag tccgcaaaaa aacgaattag aaaaagtgaa gctagaagag tacacaataa
    15721 atactatgct cgtactactc gtaatgctgt tcgtgattta agagcttcaa caaataaagc
    15781 agaagcagaa gaaatgttac caaaagtaac tgcattgctt gataaacttg ctaagaaaaa
    15841 tattattcat agcaataaag ctgcaaacat aaaatcttct atcgctaagc acgtaaattc
    15901 attagcataa aatagatttt taagtaatac actattaaaa actgttgcga aattttcgta
    15961 gcagtttttt ttatttctat attatttgta tttttacaaa agattacaaa attttatgag
    16021 aaataaaaag tctataaagt attgggcaga tgatgataaa ccccgcgaaa aaatcttgca
    16081 gaaaggaagt caagctctaa gcgatgttga gcttcttgca attttattag gttctggttc
    16141 tgccaatcag tcagcaatag aattgtcacg aagcatttta aatgacgttt caaacaactt
    16201 aaacttatta ggcaaaataa ctgctaaaga tttaatgaaa tattctggtg ttggcttggc
    16261 taaagcaagc accattcttg cagctttgga gttgggaaga cgaagaaaac aagaagaagt
    16321 tttgcaacaa aaaattatta aatcaagtca agatgtttac gactattttc aacctatatt
    16381 gggagattta agccacgaag aatttcatgt cttattttta aatagagcaa atgcagtaat
    16441 tagcagtaaa ctaataagtt ctggaggtac tacaggaact tttatagata taaaattagt
    16501 aatgaaaaac gccttagaaa atttggctca atctgtaatt attgctcata accacccttc
    16561 aggaaacgct gtgccaagcg gtaatgatat tgaagttact aaaaaaataa aaaatgcctg
    16621 tgagtttttt gatattaatt ttttcgacca tattataatt gctgacaaaa gtttttatag
    16681 ctttgcagat aaaggtttga tataaataaa ataaaaatta tgaaaagaat aattgtccct
    16741 accgtactta caatagccgt aagttttatt ttggtatatc ttttgttttt ttacgaaagt
    16801 cctaagaaag aatttatagt tggagaatat tcagaacttg ttaatccttt tattggaaca
    16861 gattatcatg gacacactca cccgtctgcg atgctgcctt ttggaatggt gcaattaggt
    16921 ccagaaacaa gattggaagg ctgggacggc tgttcgggat ttcattatag cgattcgata
    16981 atttatggtt ttactcacac tcacttgagc ggaacaggag tagctgatta tggcgatatt
    17041 ttactaatgc ctttttcaaa tttcgataat gattttagta aagataaaat tgcttcaaaa
    17101 tttagatatg acaatactgt tgcaagtccg ggatattttt caacctattt agaagataat
    17161 ggagtaaatg tttatttgac tgttactgag agagttggct ttcacaaata tgtttatgac
    17221 aatcctcaag aaaaatatat attgcttgat ttagaacata gagatgaagt tttggaatct
    17281 tatgtaaaac caatttcaaa aacaagattt gtaggtttaa gacgttcaaa atcatgggca
    17341 gaaaatcaaa ttttatattt tgttatggat ttttctcaac ctgtaagcat tgttgatttt
    17401 gaatctgatg tgattgtgaa cgatgaactt attgataata agacttttaa gctaaactct
    17461 gcaaatcaaa ctccttcaat ttatgttggt aaaaataata gcttggtttt taaagttgat
    17521 tctaattctg aaaatgaatt gcttgtaaaa gttgctttat ctgcaacagg cgaggaggga
    17581 gcaatgaaaa atttagaagc agaaattgca gattgggatt ttgaaaaagt aaaaacaaat
    17641 gctaaaaata tttgggataa agagcttagc aaaattcgta ttgaaggtgg caaagaagac
    17701 gacagaattg ccttttatac agctttatat catgcttata gcgccccaaa tatatattgc
    17761 gatgttgacg gaaaatacag agcaagagat ttagaaaact atgaaaatcc acagcacaca
    17821 acatatacag tattttcttt gtgggacaca tatagagccg cacatccttt atacacaata
    17881 gttcaggaga aaagaacttt agattttata aaaacatttt tagaccaata taaaaaaggt
    17941 ggtgctttgc caatgtggga attgtcggca aacgaaacaa tgtgtatgat aggctatcac
    18001 tctgtgccag ttatcgttga tgcgtatttt aagggaatta aagattttga tttagaatta
    18061 gctttagaag caatgatttc tgttgcaaaa actgatgaat tgggaaaagc agaatttgca
    18121 aaatacggat atattccact tgatatggag catgaatctg tgtcgaaagc tttggaatac
    18181 gcatttgatg attggtgtat tgcaatgttc gcaaaagact tgggaaaaca agatgtttac
    18241 gaagaattta tagaaagagc acaattttat aaaaatgtgt ttaatccaaa aaatggattt
    18301 atgcaatcaa agctaaatgc tgcatggcaa gagcctttcg accctaagga agtgaatttt
    18361 aattttaccg aagcaaattc atggcaatat agtttttatg ttcctcaaga tattatggga
    18421 tttattgaat tgcatggagg aaatgaaaaa tttgccaaaa aattagacga aatgttttct
    18481 gaagaaagcc aaacttctgg aaatcatcaa gttgatatta cagggcttat tggtcagtat
    18541 gctcatggaa acgagccaag tcatcatatg gcttatttgt ataattattg tggcaagcct
    18601 tggaaaactc aagcaatggc aagaaaaatt atgtcagagc tttattctgc acaacccgac
    18661 ggactttgcg gtaacgaaga ttgcggacaa atgtcggcct ggtatgtatt gtcggcaatg
    18721 ggattttatc cagtaaatcc tgcaaatgga gtttatgatt taggaagtcc acttttcgac
    18781 aaagctacta tacagcttga aaatggtaag cattttgaaa taattgcaca tgataattcc
    18841 gaaaaaaata tttatgttaa agaagtaaaa ttgaatggca gaaaatacaa aaaatcatat
    18901 atctgtcatg ctgacattat gaaaggtggc aaattggagt ttttcatggc ttcagaacct
    18961 gaaacaaata gagcaacaga ggcaaaggac atttaccaat cgcaaataac agaaaatata
    19021 attacgccag tgccttttac caatgttcct tttaggtcgt ttagagatga ggtagaattg
    19081 actttggact gtgctgacaa aaatgcagaa atttattaca gcataaacga tgaggaaaat
    19141 gcaaacccac agaaatatga aaaacctata gtaattaatt ctactacaaa attaaaagcc
    19201 tatgcaaaat cggattctaa aaagcaaagc aaaactattg aagcagaatt aatttttatt
    19261 cccgacaata gaactattaa gcttataagt gaatatagtc ctcaatatac agcaggtggc
    19321 gacaatgctt taattgactt tattaaaggc gaagcctggt ttagaagtgg cttgtggcaa
    19381 ggctacaggg gacaagattt tgaagccata gttgatttgc aagaagtgaa agaaatcaat
    19441 cttattaatg caagattttt gcaagatatt cgagcttgga tatttttccc taagaaagta
    19501 agttattttg cttctaatga tggagttaac tttacaaaaa tctatgaatc tgattcgcaa
    19561 atgcctgata atgactatga agtaaaaata caagaattta aaaacgagcg tgaaaatttt
    19621 aaagccagat atataaaagt tgtagctaaa agttatggaa aattaccaga atggcattta
    19681 agtgcaggtt ataattcttg gatttttatt gatgaaattg aaatttatta atgaaaagaa
    19741 tacttttttt agttatttgc atatcagttt tttcgcttat ttcttgtgaa aattacaagg
    19801 caaaaaagaa aattaaagca gatttaagcg atgtgcaatt tgaggataaa gaccttaatt
    19861 acgacagcat ttttgcctta gttcccgact ataacgaaat gttaggcatt ttgaaagacg
    19921 ttagggtagg ctatagttct aaaattttgt tagactctaa atcagcagaa atgtttttaa
    19981 cttctaaaga ggtgagtttt gctttaggaa tgtttgtagc tgatttggct tatgttagat
    20041 attttgaaaa agtgcaatta tgtactgaat atttagaaag tttaaaaatt ttgacagaac
    20101 aattagcaat tagcaataaa aattttgaaa gtcttgttaa aaaagtggaa actaatattc
    20161 aggatagaga tgaattgttt ttagttgttg atagtttgtt aggcgaggca aataaaattt
    20221 tcacaaagtc agagcagcac agtttatctt gtttggcatt gtcgggtttt tggttagaaa
    20281 tatcttattt aggcttaaat tccataaatg cagaaaatat tgaaaataat tcagattttt
    20341 tccactctca tttcgaaatc ctcaagctaa taaaccaact ttactcctgc ttagaagacg
    20401 aaagctttat caatccccta aaacaaaatt ttcttgaatt agaagcaaaa ggcttggaaa
    20461 atttggagtt ggaggaggat attttgaaga ttagaagtga ttatgttaag tgaggggatg
    20521 tagaaaattt ttatattttt tgttatttat aatgccaaga tttaagtttt gtcatacgga
    20581 taaggagata gatttatttt aacaatgttt atgagtttta ctctaccctt ttatctaata
    20641 tccatatttt atctatattt tatctatatg ttctttcaca aattagtatg agttttaaga
//
LOCUS       DAOPNX010000042        19571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_3406_length_19571_cov_6.143318, whole genome shotgun sequence.
ACCESSION   DAOPNX010000042 DAOPNX010000000
VERSION     DAOPNX010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19571
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3406_length_19571_cov_6.143318"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1239)
                     /locus_tag="PKN53_06135"
     CDS             complement(52..1239)
                     /locus_tag="PKN53_06135"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOD88562.1"
                     /translation="MKKVFKILLLIVALAIVVLSSGRIFNYEIKSGSKPNSNKNKEVN
                     EKTKIFPELVADYNYYLLDSIKDSYTLFNSNDEQLATVIFSFPYCDSIKGFGDNIPLA
                     IVFNSNDEVEQIYFFPNNETKSWMNMLEKDGFFETWNSLSAEKALDLEVNAVSGATYS
                     SKAIIQIVKHRLSLYTDSSSSDFSSNWVNVLGLILSFVVLIFAIISFFKPKYGRKFRI
                     YLLIASVGILGFWQGKFLSLALFHYWLLNGFNIWEQIFLFTVLILAVILPLITNKSFY
                     CQYLCPYGAAQELVSKLNKKKKINLGNKITKYLKYVKFAYLFVIFLLIVLSVDVVLED
                     FEPFSAFKFQFASLTVLILSVVMLVLSAFINKAWCRYFCPTGAILSLFRTKTSRSKDD
                     CEN"
     gene            complement(1252..1968)
                     /locus_tag="PKN53_06140"
     CDS             complement(1252..1968)
                     /locus_tag="PKN53_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOD88563.1"
                     /translation="MIKEMNFKSCLYLIPNLIGATDVDDVLPKNLYSQISHIRHFIVE
                     DIRNARRFLRSLSKDFVIDDLTFYELNKHTEIEQIEDYLKVSEQGLDIGLLSEAGLPC
                     VADPGNLIVQLAHKKNIRVKPLSGPSSIFMALMASGMNGQNFAFNGYLPIDNSQKISK
                     IKSLEARSRKENQSQIFMDTPFRNDKLLSDLLSTLNPETYLCVASNISCEDEMIIRKK
                     VADWKKINISLNKKPCIFII"
     gene            2070..2828
                     /locus_tag="PKN53_06145"
     CDS             2070..2828
                     /locus_tag="PKN53_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD88564.1"
                     /translation="MKAIILGTGTSLGVPVIGCKCEVCTSTNSKDKRLRTSIFLKLDN
                     TNIIIDAGPDFRQQVLRENITKIDAVLITHEHRDHIGGLDDLRPFSYLLNSAIPIYAE
                     KRVADAIINEFSYSFNTNYPGIPKFEINTISEEKFKINDIEILPIRAMQGKLPILGFR
                     INDFVYITDASFISEKELKKIEDCKILIINALRHEKHDKHFSLDEAINIAQSTKAEQV
                     YLTHISCSMGLYDKINSELPEKIQLAYDGLQINF"
     gene            2980..4179
                     /locus_tag="PKN53_06150"
     CDS             2980..4179
                     /locus_tag="PKN53_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOD88565.1"
                     /translation="MKKFLLGLLAISFLSVSVIAQDDTEKKDESYVFTLKKEVKTTPV
                     KDQYRAGTCWSYSGLAFIESELLRLGKPEIDLAELFIVRYTYNDKAEKYVRMHGEINF
                     SGGGAFHDVFNVIKKHGIVPEDVYTGLNYGETNHVHGELDAVLKGYVDAVIKNGNKKL
                     STAWLRGYNGILDAYLGEVPEEFTYEGKKYTPQSFAEYLGINTDDYINITSYTHHPFY
                     EKFILEIPDNWAWGTAYNLPIDEMMQVLDNAINNDFTVCWGSDVSEKGFKWTRGFAVI
                     PEEDRPDLDGLERDKWEKLSAREKESLLYALDKPVPEKTITQEMRQEAFDNYLTTDDH
                     GMLICGMGTDQNGTKYYYVKNSWAASNEYDGYFYASESFVKYKTMNIVVHKDAIPKDL
                     KKKMGIK"
     gene            4349..4768
                     /locus_tag="PKN53_06155"
     CDS             4349..4768
                     /locus_tag="PKN53_06155"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD88566.1"
                     /translation="MPEQQDIEYKSAWHDDYLKWICGFANAQGGTIYIGKDDNGNVVG
                     INDYKKLMDDIPNKIRNCMGITVEVNLHEENEMYYIKIVTPPYSVPISLRGRYYYRIG
                     STKQELTGTSLNEFLLKKSGKTWDDVVEPRATLDDID"
     gene            4793..4999
                     /locus_tag="PKN53_06160"
     CDS             4793..4999
                     /locus_tag="PKN53_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88567.1"
                     /translation="MAKEKGRLPEVDGLSTEQLFDKLRLIENGQIKRAALILFGKDPG
                     KFYPSINVRIGRFKDELNLIFSGK"
     gene            5019..5672
                     /locus_tag="PKN53_06165"
     CDS             5019..5672
                     /locus_tag="PKN53_06165"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD88568.1"
                     /translation="MYQAVLNQINHKFIIKNISFEGMHRIETPEYPREAMREAILNAL
                     VHRNYMGVHTQIRVYDDKISFWNDGGLQRPLTVESLKRPHSSRPRNVLIADVCFKGGL
                     IDAWGRGTIKIMETCKQAGLPEPEIIELDGGLLVTMFKNKLTKEQLIKLGLNKRQLKA
                     VEYVKEKGKITNKEYQKLNGVSKVTAYRDLTELIEQYKLFERKGDIGAGTSYFLIGS"
     gene            complement(5915..6484)
                     /locus_tag="PKN53_06170"
     CDS             complement(5915..6484)
                     /locus_tag="PKN53_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88569.1"
                     /translation="MLLDSVQGDLNKDSYKDLVLVIQHIDSVMLDEYADLAQPRMLLI
                     LFYDNESNLYNLAEKSESFILPHDNPNMTDPYVGMSISNKGVLKIEFEFFATMGSYGS
                     STSSYKFRYQNNEFALIGADDYYLHRATGEFGERSFNFLTKKLKVTEGHIDSDKKKVV
                     WRDLDIKELKTLKTFKKPYSWEVEKDSNI"
     gene            6799..10554
                     /gene="dnaE"
                     /locus_tag="PKN53_06175"
     CDS             6799..10554
                     /gene="dnaE"
                     /locus_tag="PKN53_06175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOD88570.1"
                     /translation="MIDFTHLHVHSQYSILDGAASINALVKKTKDSGMTSLALTDHGT
                     LLGIKEFHEVCLKNGIKPILGCEAYVARKSIHDKYDKEDLSGNHLVILVKNLTGYYNL
                     LKLMSIASTDGFYQRPRIDKELLEQYKEGLIVSSACLGGEIPRLIDNDRIDEAKEVIL
                     WYKEVFADDFYLEIMYHPSENPEQKFNVSDKQLKVNKKILELGKELDVKVIATNDVHF
                     TNEEDAEAHDVLICLTTNADFDDPDRLRYTRQEWLKTPEEMLALFPNNPETLSNTMEI
                     ADKIEIYEINSDPIMPEFPIPEEFGTIEQWKEKYTEQDLIKEFTEDRYNALHGYDAVL
                     RIKFESDYLTHLVLIGAKERYGENYSEEIKERIDFELNTIKTMGFPGYFLIVQDFIAA
                     ARKMGVFVGKGRGSAAGSVVAYCTKITDVDPIEFNLLFERFLNPDRISMPDVDIDFDD
                     DGRALVIDYVTEKYGQEKVAHICTIGTMKAKGALRDVSRILGIPLPTVNALTKKIPTL
                     GSLSNAYEIILETEQKTGSIDKAIAAIEKDYKTAINEDNDKEANILNTRLIIAQEIKS
                     AREVNDEKLLKAIAIACTLEGSVRQTGVHACGMLIGKNNLENHIPLMRVKDDSKLYAT
                     QYEGSYVESIGLLKMDFLGLRTLSIIKETLKNIEISRKEKISVDNLPLDDKKTLEIFK
                     TGATTAIFQFESEGMKSHLRSLKPNHFNDLVAMNALYRPGPMEYIKDYIDRKHGIQHT
                     EYDHPIMEKFLKDTYGITVFQEQVMLQARALANFTRGESDSLRKAMGKKLSAEMEKYH
                     QKFIEGCKSNPQFIEGCKVVKKDPDELIDKIWKDWSNFAKYAFNKSHSVCYAYIAFQT
                     AYLKAHYPAEFMAANLTRNISEMDKITVLMEECRRMKIKVLGPDVNESYNDFTVNKKG
                     NIRFGLQGIKNVGTNAVANIIEEREKNGAYKDIFDFVSRVNLNAVNKKNIEALVHGGA
                     LDCFSELQRVQYFIGTNGFDSFIEELIRFGNNMQNSAPASTTLFGDSFAIEVKKPEIP
                     ILPEVNIFEVLKNEKEHIGMYLSGHPLDPFKFIINNYKTIPLIELENEDNKKRFANIK
                     VAGYITDVTERLSKTGNPYAKITMIDYSSTFSFSVFGKKYAELKSYFVKGYAILMNCG
                     FEEKTHEPGLRFNVYKINLLADMKNDFFNELILKLPTENITEEFISEFLKVLKSSKGK
                     ITLSLSLEDKKNKSEVTVFSRNHQIKLSDKLIEYLESNKYFNYIKLN"
     gene            10687..11004
                     /gene="trxA"
                     /locus_tag="PKN53_06180"
     CDS             10687..11004
                     /gene="trxA"
                     /locus_tag="PKN53_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOD88571.1"
                     /translation="MALEITEKNFDELVVNTDKPVVLDFWAEWCGPCRMIAPLIEEMH
                     NEYGDKAVIGKVDVDSNNSIAVKYGIRNIPTILFLKDGEVVDKVVGAVPKATLTAKLD
                     ALL"
     gene            11043..11462
                     /locus_tag="PKN53_06185"
     CDS             11043..11462
                     /locus_tag="PKN53_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOD88572.1"
                     /translation="MTINEIQDEIIQEFEVFDDWMDRYALLIEIGKELAEMPSEYKTD
                     QNLISGCQSNVWFHAEFKEGKIYFTADSDAVITKGIAALLIRVLSERTPKEILETDLY
                     FIKKTGLQQHLSPTRSNGLLSMIKQLKLYAMAFNALK"
     gene            11478..11783
                     /locus_tag="PKN53_06190"
     CDS             11478..11783
                     /locus_tag="PKN53_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HOD88573.1"
                     /translation="MTEIEINIVENLKTVYDPEIAINVYDLGLIYRIDVNSDYDVDVD
                     MTLTAPNCPMADDIIKNVNDAIKNVENVRSVNINLVFDPPWSKDMLSDEALLELGLL"
     gene            11842..12795
                     /gene="ftsY"
                     /locus_tag="PKN53_06195"
     CDS             11842..12795
                     /gene="ftsY"
                     /locus_tag="PKN53_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006282862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOD88574.1"
                     /translation="MAFFGLFGKEKKENLDQGLEKTRSSIFSKLNRLIFSKSKIDDDV
                     LDELEEILISSDVGVETTVRIIEKIQENVKKEKYQSSEELDEILRSTIASLLEENETN
                     QTDEDEIFNSAKPYVIMVVGVNGVGKTTTIGKLAYKFKNAGKSVYIGAADTFRAAAID
                     QLQIWADRVGVPMIRQDMGSDPASVAFDTLSAAKKAEADVVIIDTAGRLHNKINLMNE
                     LTKIKRVMQKVIEDAPHEVLLIIDGSTGQNAYEQAKQFTKATEVTALAVTKLDGTAKG
                     GVVIGISDQFKIPVKYIGIGEGIEDLQIFDKKEFVNSLFKK"
     gene            12953..14674
                     /locus_tag="PKN53_06200"
     CDS             12953..14674
                     /locus_tag="PKN53_06200"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOD88575.1"
                     /translation="MKKFGLKIVVLIAFVSLINYSGFSQNNSSKASVETAIAISKNFM
                     PSASKSGINAEIKSISSIKNSNDQEKIHVLNFANGGFMLVSSDNRSVPVLAFSDEGEF
                     NFNENTAPATKMWINQYLRQLDYIEENNVQATEQIQQLWADMLNGNYQSSTKKVDQLV
                     LTKWNQDWPYNMFTPEHPEGNNGHTYTGCVATAMAQIMKYWEYPDTAKGVVNYHWGDY
                     YEVDLNKESYDYSLMPNFFMPNSTQQQKEEVARLMFHCGIAVHMDYGHESSGSQTVYA
                     WQAFKENFKYRSGSYFTYKETMSDAEWKFRLKYDLDLGRPIMYAGVSEEEGGHAFICD
                     GYRDTSFFRFNWGWGGSKDGFYYLDKINPQMEFPFAQSAILELAPHGADFCKQNIVMT
                     LPSYSFDDGSGPNLYAKNTNCSWLINPDMTNADFLRLTFDRFDLASGDTLKIWKGNPI
                     NGLIELVGEFTYNPGEVITEGNKIYLEFTTDGQAQAQGFQAHYEVVVLGITENTNAVL
                     KVYPNPTTNILQVEGLQTENIQIMDISGKIVLNANVSEKSIDVSSLKPGLYIIKANNQ
                     VARFIKE"
     gene            complement(14754..16475)
                     /locus_tag="PKN53_06205"
     CDS             complement(14754..16475)
                     /locus_tag="PKN53_06205"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOD88576.1"
                     /translation="MKNYLLIVLVFIFTPFFSDACTNYLVTKTASVDGSTMISYAADS
                     HIRYGELYWRPAADWEKGNMRIIYDRGTNKPLGKIPQVPHTYKVVGFMNEHQLAIGET
                     TFGGRDELVDTTGIIDYGSMMFIALERAKTAREAIKIMIELFNEYGYASSGETFSIAD
                     PNEVWIVELVGKGVEQVYDKKQKKYVNKNKGIVCVAIKIPEGYVSAHANHSRITNFPL
                     ENMKNSISCKNLDKIFDEHIEVVYSHDVISFARDKKYFSGKDEDFSFSQAYAPINYSA
                     ARGCELRVWAMFNEISDDMAKYWDIACGNDLENRLPLYIKPNRKLSQLDLFKFFRNYM
                     QGTELDMTKDIGAEPSGMPYRWRPLTFKYEGKEYLNERVTVTQQTGFSFVAQMRSWLP
                     NEIGGIYWLGVDDAGSCVYTPFYVGITKPAEKWAEGYGDILTYKDDAAFWVFNRLAHF
                     KYLFYNRVMPEIEKHQSFLENKYVEYVNIIDETALKLYKQSPEKAAEFLTEYSCNTAN
                     SLVDYWKEFNNFLLVKYLDGNVKPEENGEFLRNPWGYPKKIIWPGYSDEWKKTLIDNT
                     GDKFLMR"
     gene            16692..17528
                     /locus_tag="PKN53_06210"
     CDS             16692..17528
                     /locus_tag="PKN53_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88577.1"
                     /translation="MRKILLFTLAFSIFFVGCKKEGVYNPNKKIKRIYYEYGLNTGTK
                     TLSQEWTWEKDKLVKIKHSFYTEILSYDNKNRLIKIQDFDFNDRYEIIYDKKLISKIE
                     YYIGNSLCQTMNFKYDKDKIVEITIKNIDNNKMLENKSIKPDSFISTILPFKIPDKAM
                     RTKSAISSTYIITYEYDGDNISKTKYIDDDNQVHEYTQENYDNKLNPFYNSRFLSNGL
                     GIGFSKNNVGKATTITSDNDVKTIEFEYVYDGKFPTEVIEKESSSATNEFSINKTYYE
                     YQ"
     gene            17618..18478
                     /locus_tag="PKN53_06215"
     CDS             17618..18478
                     /locus_tag="PKN53_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88578.1"
                     /translation="MRKILLFTLALSIFFVGCKKEGVYNPKEKIKRVYYERGLDASTK
                     RLVEEWTWEKNNLVKIDYYDDNYGIIKIDYTEILSYDDKNRLTRIQDFVFNERYEITY
                     DKKLISKIELYIDNELYQIMNFKYDRNKIVEVSVIEIDNSKILVSKSNKPQSYISKIL
                     PYEIPENTMRTKAASLSYIYTFEYDGDNLSKVKYIDDDNRVREYNYENYDKYKNPFYS
                     SRFISNNIESGFSKNNVGKITTTTIGNTVRTAEFEYVYDGKFPIEIIERTTYSGSDLI
                     WIYKTYYEYQ"
     gene            complement(18584..19459)
                     /locus_tag="PKN53_06220"
     CDS             complement(18584..19459)
                     /locus_tag="PKN53_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOD88579.1"
                     /translation="MDTQLIIIIGAIVLLIFFAGIKIVRPTHRGLIERLGKYRKFAKP
                     GFHWIIPVIDKMYRVNITEQMVDAQPQEIITNDNLNASVDAQVYFKVNFDEESVKNSQ
                     YNVNNYQWQIVNLARTTLRNIIGTLTLKAANSERGKINAELHKTLCNETRSWGIDIVR
                     TELKQIDPPHDVQETMNKVVKAENEKIAAIDFATAAEISADGTKRAKIKEAEGVKQAK
                     ILYAEGEASAIKLVNEAADQYFVGNAQLLKKLETIEKALKDNSKIVIPEGSDLVNIIG
                     EMAGGVLPIEKNKTK"
BASE COUNT         7414 a         2834 c         2954 g         6369 t
ORIGIN      
        1 aatatttttt gttattttca tttttataaa acagaaataa ataaattttg tttaattttc
       61 acaatcatct ttgctcctac ttgtctttgt tctaaataaa gataaaattg ctcctgttgg
      121 acaaaaatat cgacaccaag ctttatttat aaaagcagac aatacaagca ttactactga
      181 tagaataagc acagtcaaag atgcaaattg aaatttaaaa gcagaaaaag gttcaaaatc
      241 ttctaacact acatctacag acagaacaat taaaaggaat attacaaaaa gataagcaaa
      301 ttttacatac tttaaatatt ttgtgatttt attccccaaa tttatttttt tctttttatt
      361 tagttttgaa accaattcct gagctgctcc ataaggacaa agatattgac aatagaatga
      421 tttatttgta attaaaggta aaatcactgc caaaatcaaa actgtaaata aaaatatttg
      481 ctcccaaata ttaaaaccat ttaacaacca ataatggaat aaagctaaac ttaaaaattt
      541 tccttgccaa aatcccaaaa tccctacaga agcaattaat aagtatattc taaattttct
      601 tccatatttt ggtttaaaaa atgaaattat cgcaaaaatc aaaactacaa aagacaaaat
      661 caaacctaat acattaaccc aattgcttga aaaatccgaa gaacttgaat ctgtatataa
      721 tgaaagtctg tgttttacta tttgaataat tgctttgctt gaataagttg ctccactaac
      781 agcatttact tctaaatcca aggctttttc tgccgacaaa ctattccaag tttcaaaaaa
      841 accgtctttt tccaacatat tcatccaaga ttttgtttca ttatttggaa aaaaataaat
      901 ctgctcaact tcatcattac tattaaaaac aattgctaaa ggaatattat cgccaaaacc
      961 ttttattgaa tcacaataag gaaaactaaa aatcactgtc gctaattgct catcattaga
     1021 attaaataaa gtataagaat cttttatact gtcgagtaaa taataattgt aatcagctac
     1081 taattcaggg aaaattttag ttttttcgtt gacttcttta tttttatttg aattaggttt
     1141 tgaaccagat tttatttcgt aattaaaaat tcttcccgaa cttaacacaa caattgcaag
     1201 agcaacaata agcaataata ttttgaatac ttttttcata cttacacaaa actaaataat
     1261 aaaaatacaa ggttttttat ttaatgaaat atttattttt ttccaatcag caactttttt
     1321 gcgaattatc atttcatctt cacaagaaat atttgaagct acacacaaat aagtttcagg
     1381 atttaatgta gaaagtaaat cacttaataa tttatcattt ctaaatggtg tatccataaa
     1441 aatttgtgac tgattttctt tgcgagaacg agcttctaag ctttttattt tagaaatctt
     1501 ttgagaatta tcaataggta aataaccatt aaaagcaaaa ttctgaccat tcattcccga
     1561 agccattaat gccataaaaa ttgaagaagg accagaaagt ggttttactc ttatattttt
     1621 tttatgagct aattgtacta ttaagtttcc tgggtcagca acacaaggca aaccagcttc
     1681 actcaacaaa cctatatcta aaccttgttc agaaactttt aaataatctt caatttgctc
     1741 aatttctgta tgtttattca actcataaaa agttaaatca tcaataacaa aatcttttga
     1801 caaacttctt aaaaaccttc ttgcattgcg aatatcttca acaataaaat gtctaatatg
     1861 agaaatttga gaatataaat tctttggcaa aacatcatca acatctgttg caccaatcaa
     1921 attaggaatt aaatatagac aagatttaaa gttcatttct tttatcatta ttttgcaaag
     1981 ttaaaataaa acttagttaa aaaactattt ttacctatca ttaatatttc gcttaacaat
     2041 atttttacta aatttgtatc aaaatattta tgaaagcaat tattttaggt acaggaactt
     2101 cgcttggcgt gccagttatt gggtgcaagt gcgaagtatg cacttcaaca aacagcaaag
     2161 acaaaagact tagaacctca atttttctaa aattagataa tacaaatatt attatagatg
     2221 caggacctga ttttaggcaa caagtgctaa gagaaaatat cacaaaaatt gatgctgtcc
     2281 taattactca cgaacatcgc gaccatattg gtggtttgga cgacctaaga cctttcagct
     2341 atttgttaaa ctcggcaata ccaatttacg ccgaaaaaag agttgccgat gcaattataa
     2401 atgaattctc ctactctttt aatacaaatt atcctggaat tccaaaattt gaaataaata
     2461 caatttctga agaaaaattt aaaataaacg acatagaaat tcttccaatt agagcaatgc
     2521 aagggaaact accaattctt ggttttagaa ttaatgattt tgtttacatt acagatgcta
     2581 gctttatttc tgaaaaagag cttaaaaaaa ttgaagattg caaaattctc ataatcaatg
     2641 cattgaggca tgaaaaacat gataaacatt tttctttaga cgaagctata aacatagctc
     2701 aaagtacaaa agccgaacaa gtttatttaa cacatatcag ttgttctatg ggtttatacg
     2761 ataaaataaa ttctgaattg cccgaaaaaa ttcaattagc ctacgatgga ttacaaataa
     2821 atttttagaa caacacaaaa gctttgctaa tcatatcata cttgttgttt taactttttt
     2881 ctaagtatta taaaattcaa atattattgt ataaacaaaa acttttatta aatttgtgca
     2941 taaataaatt aatattaaaa ttaaataaaa tctgaattta tgaaaaagtt tttacttgga
     3001 ttattagcaa tttcgttttt aagcgtatcg gtaattgctc aagacgacac tgagaaaaaa
     3061 gatgaatctt atgtattcac cttaaaaaaa gaagttaaaa caacacctgt aaaagaccaa
     3121 tacagagctg gtacttgctg gagctatagt ggtttagcat ttattgaatc tgaactttta
     3181 cgccttggca aaccagaaat cgaccttgca gaacttttta ttgtacgtta tacttacaac
     3241 gacaaagctg aaaagtatgt aagaatgcat ggcgaaatta attttagtgg tggtggtgct
     3301 ttccatgatg tgtttaatgt aattaaaaaa catggaattg ttcctgaaga tgtttacaca
     3361 ggcttaaact atggcgaaac taatcatgtt cacggtgaat tagatgctgt tttaaaaggc
     3421 tatgttgacg ctgttattaa aaatggtaat aaaaaacttt ctacagcttg gctaagaggc
     3481 tacaacggca ttttagatgc ttatcttggc gaagttccag aagagtttac ttatgaaggt
     3541 aaaaaataca ctccacaatc atttgcagaa tatttaggaa taaatactga tgattatata
     3601 aatattactt catatacaca tcatccattt tatgaaaaat ttattcttga aattcctgac
     3661 aactgggctt ggggaactgc ttacaactta cctattgacg aaatgatgca agttttagac
     3721 aatgctatta acaacgattt tacagtatgc tggggttctg atgttagcga aaaaggattc
     3781 aaatggacaa gaggttttgc tgtaattcca gaagaagaca gacctgactt agatggttta
     3841 gaaagagata aatgggaaaa actttctgca agagaaaaag aaagtctttt atatgcttta
     3901 gacaaaccag ttcctgaaaa aactattact caagaaatga gacaagaagc ttttgacaat
     3961 taccttacta cagacgacca cggaatgttg atttgtggaa tgggtacaga tcaaaacggc
     4021 acaaaatatt attatgtaaa aaactcttgg gcagcaagca acgagtatga tggatatttt
     4081 tatgcttcag aaagttttgt aaaatataaa actatgaata tagttgttca taaagatgct
     4141 attccgaaag atctaaaaaa gaaaatggga attaagtaag acgatgcttc aggagtgtca
     4201 aactgctggc ctgagtttat aaagttaaaa gttcataaag tttttaaagt cggttttata
     4261 acaaaatgtg tcaacttttc ttaggacgcg acatttttga attttatatt ttggtttact
     4321 tttacgaagg catttaatta taatacctat gcccgaacaa caagacatag aatataaaag
     4381 tgcttggcac gatgactacc tgaaatggat ttgtggcttt gcaaacgcac aaggtggtac
     4441 tatttacatt ggtaaagacg ataatggaaa tgttgtaggc attaatgatt acaaaaaatt
     4501 aatggacgac attccaaaca aaattcgcaa ttgtatggga attacagttg aggtaaacct
     4561 gcacgaagaa aacgaaatgt attacattaa aattgttaca ccgccttatt ccgttcctat
     4621 ctcattacgt ggtagatact actaccgtat cggaagcacc aaacaggaat taacagggac
     4681 ttcgcttaat gaatttttat taaagaaatc gggcaaaacc tgggacgatg ttgttgaacc
     4741 acgtgctaca ttagatgata ttgactaaac agcagtagcc aaatttttgg taatggcaaa
     4801 agaaaaggga cgtttacccg aagtagacgg attatcaact gaacaacttt ttgacaaact
     4861 gcgtttaata gaaaacggac aaataaaacg tgcagctttg attttatttg gcaaagaccc
     4921 tggcaaattc tatccgagta ttaatgttag aattggtcgt tttaaagatg agttgaattt
     4981 aattttttca ggaaagtgag gaaggtaata ttatcaattt gtatcaagca gttttaaatc
     5041 aaatcaatca taaatttatt atcaaaaaca tttcttttga gggaatgcat cgtatcgaaa
     5101 caccagaata tcctagagaa gcaatgcgtg aagctatttt aaacgctttg gtacatcgca
     5161 attatatggg cgttcatacg caaattcgtg tttatgatga taaaatttct ttctggaatg
     5221 atggtggatt acaaagaccg ttaactgttg aatcgttaaa acgaccacac tcatcacgac
     5281 caagaaatgt tttgattgct gatgtttgtt ttaaaggcgg tttaattgat gcttggggac
     5341 gtggcacaat taaaattatg gaaacctgca aacaagcagg acttcctgaa cccgaaataa
     5401 ttgaactgga cggaggtttg ttggtaacaa tgtttaaaaa caaattgaca aaagaacaac
     5461 ttatcaaact tggattaaac aagagacaac taaaagcggt tgagtatgta aaagagaaag
     5521 gaaaaattac gaataaggaa tatcaaaagt taaatggtgt ttctaaagta actgcatata
     5581 gggatttgac agaacttatt gaacaatata aactttttga acgaaaagga gatattgggg
     5641 caggaacgag ttacttttta attggttcat aattggttca attggttcat taatagttct
     5701 ttaatagaaa gaaaagataa catctaataa ataacgcaac gctatttcta aacacattat
     5761 caaattcgta caaagtcaac cccctactgc ttaaattctt cctcgctaat ctcacaaatt
     5821 tgtctcttcc aaaaaaagtt ttcatattat tatggaaatt caactttaaa aactttacaa
     5881 actttcaact aattatccta cacaaaatca acttttatat attactatct ttctcaacct
     5941 cccatgaata tggtttttta aaagttttaa gtgtttttaa ttcttttata tcgaggtctc
     6001 gccaaacaac ttttttcttg tcagaatcga tatgaccttc agttactttt agtttttttg
     6061 taagaaaatt aaaacttctt tccccaaatt cgcctgtagc acgatgcaaa tagtagtcat
     6121 cagcaccaat aagagcaaat tcattgtttt gatagcggaa tttatacgaa cttgttgacg
     6181 agccataact tcccatagta gcaaaaaact caaattcgat tttaagtaca cctttgttac
     6241 tgatagacat tccaacatat ggatctgtca tgttaggatt atcgtgaggt aatataaaac
     6301 tttcactttt ttctgctaag ttgtatagat ttgattcatt atcataaaaa agaattaaca
     6361 acattctggg ttgtgctaaa tctgcatatt cgtcaagcat gacgctatct atgtgctgaa
     6421 taactaaaac taaatcttta tagctatcct tatttaaatc gccttgtaca gaatctaata
     6481 acatccaacc ttatggaata aagttttcaa catcttttcc ctgcgcatta atgttagggt
     6541 acaaaaagtc ctgaccatat gcaaatccaa atgtcagcac aaataatact gtaatatttt
     6601 ttttcatttt attgcttatt tttttgtttt ccacaaactt ataaatttta tttttataaa
     6661 cataacaaaa taagtaccat tttttattaa aaattatttg ttaattactt tgggataaaa
     6721 attttgtatt taatttttta attgctgcat ttaagttaat tttatagcct tgcgtttttt
     6781 tgaaaaagat tttagaaaat gattgatttt acacacttac acgtccactc acagtactct
     6841 attttagatg gagcagcatc aattaatgcc ttagtaaaaa aaactaaaga tagcggaatg
     6901 acttctttag ctttaaccga ccatggtact ttattaggaa ttaaagaatt tcatgaagtt
     6961 tgcctaaaaa atggcataaa accaatctta ggttgtgaag cttatgttgc aagaaaaagc
     7021 attcatgaca aatacgacaa agaagatttg tctgggaacc acttggttat tttagttaaa
     7081 aacctaactg gttactataa tttattaaaa ttaatgtcta ttgcaagcac agatggcttt
     7141 tatcaaagac ctagaattga caaagaattg ttagaacaat ataaagaagg tttaattgtt
     7201 tcgtctgctt gtttgggtgg tgaaattcca agacttatag ataatgatag aattgatgaa
     7261 gctaaagaag taattttatg gtataaagag gtttttgcag atgattttta cttagaaatc
     7321 atgtatcatc caagcgaaaa tccagaacag aaattcaatg tttcagacaa acaattaaaa
     7381 gtaaataaaa aaatacttga acttggtaaa gaacttgatg ttaaagtaat tgctacaaac
     7441 gacgtgcatt tcaccaacga agaagatgcc gaagcacatg atgtattaat ttgcctaact
     7501 actaatgctg attttgacga ccctgaccgt ttaagatata cacgacaaga atggttaaaa
     7561 actccagaag agatgttggc tctgtttcca aacaatcccg aaactctatc aaacacaatg
     7621 gaaattgccg acaaaataga aatttatgaa atcaacagcg accccattat gccagagttt
     7681 ccaattcccg aagagtttgg aactattgaa caatggaaag aaaaatacac agaacaagat
     7741 ctaattaaag aatttactga agatagatat aatgctttgc acggctacga tgctgtttta
     7801 agaataaaat ttgaatctga ttatttaaca cacttagtat taataggtgc aaaagaaaga
     7861 tatggcgaaa actattctga agaaataaaa gagagaatag attttgaact caacacaata
     7921 aaaaccatgg gttttcctgg atatttcctt attgttcagg attttattgc agcagcaaga
     7981 aaaatgggag tttttgtggg aaaaggtaga ggttcggcag ctggctcggt agtagcatat
     8041 tgtacaaaaa ttactgatgt tgacccaata gagtttaatt tactttttga gagattttta
     8101 aaccctgaca gaatttcgat gcctgacgtg gatattgact tcgacgatga tggaagagct
     8161 ttagtaattg attacgttac agaaaaatat ggacaagaaa aagtagctca catttgtact
     8221 attggaacta tgaaagcaaa aggagcttta agagatgtat caaggatatt gggcatacca
     8281 cttccaacag taaatgctct aactaaaaaa attccaactt taggctcttt aagcaatgct
     8341 tatgaaataa tattagaaac agaacaaaaa acaggctcta tcgataaagc catagcagct
     8401 atagaaaaag attacaaaac agctataaat gaagataatg acaaagaagc taatatttta
     8461 aacacaagac ttataattgc tcaagaaata aaaagtgcaa gagaagtaaa tgatgaaaaa
     8521 ttgctgaaag ctattgctat tgcatgtaca ttagaaggct ctgtaaggca aacaggagta
     8581 catgcttgcg gaatgttaat aggaaaaaac aatcttgaaa atcacattcc attaatgagg
     8641 gttaaagatg attctaaact ctatgctaca caatacgaag gaagttatgt tgaatctatt
     8701 gggctattaa aaatggactt tttaggttta agaactttat caatcataaa agaaacacta
     8761 aaaaatattg aaatatctag aaaagaaaaa attagtgttg ataatttacc attagacgat
     8821 aaaaaaactt tagaaatttt caaaacagga gcaacaactg cgatattcca atttgagtcg
     8881 gaaggtatga aatctcattt acgttcttta aagcccaatc actttaatga tttagttgct
     8941 atgaacgctc tgtaccgtcc tggtccaatg gaatatataa aagattacat tgacagaaaa
     9001 catggaatcc agcacacaga atatgaccac ccaataatgg agaaattttt gaaagacact
     9061 tacggaatca cagttttcca agaacaggta atgttacagg caagagcact tgctaatttt
     9121 actagaggtg aatctgacag tctgagaaaa gcaatgggaa aaaaattaag tgctgaaatg
     9181 gaaaaatacc atcaaaagtt tattgaaggc tgtaaaagta atccccaatt tattgaaggc
     9241 tgtaaagttg ttaaaaagga ccctgatgaa ttaatagata agatttggaa agactggtct
     9301 aattttgcta aatacgcatt taataaatct cactctgtat gctatgctta catcgcattt
     9361 caaactgcat atctaaaagc acactatcca gccgaattta tggcagccaa cctcacacgc
     9421 aatatttctg aaatggacaa aataaccgtg ctaatggaag aatgcagaag aatgaaaatc
     9481 aaagtattag gtcctgatgt aaacgaatct tataatgatt ttactgtaaa caaaaaagga
     9541 aatattagat ttggattaca aggcattaaa aatgttggaa ctaatgcagt tgcaaatatt
     9601 attgaagaaa gagaaaaaaa tggagcatac aaagacatat ttgattttgt ttcaagagta
     9661 aatctaaatg ctgtaaataa gaaaaacata gaagccttag ttcatggcgg tgctttagat
     9721 tgctttagcg aacttcaaag agttcaatat tttattggaa ctaatggttt tgatagtttt
     9781 attgaagaac ttattagatt tggcaataac atgcaaaact ctgcacctgc ttccactact
     9841 ctatttggcg acagttttgc tattgaagtt aaaaaacctg aaattccaat tttgccagaa
     9901 gtaaatatct ttgaagtttt aaaaaacgaa aaagaacata taggcatgta tttgtcagga
     9961 cacccattag atccattcaa atttattatt aataattata aaactatacc actaatagaa
    10021 ttagaaaatg aagataataa gaaaaggttt gcaaatataa aagttgctgg ttacattaca
    10081 gatgttactg aaaggttgag caaaactgga aacccttacg caaaaattac tatgatagat
    10141 tattcttcaa ctttttcttt ttcagtattt gggaaaaaat atgcagaact aaaaagttat
    10201 tttgtaaaag gatatgccat tcttatgaac tgtggctttg aggaaaaaac acatgaacca
    10261 ggactaaggt ttaatgttta taaaataaac ttacttgctg atatgaaaaa tgattttttc
    10321 aatgaactta tactaaaact tccaactgaa aatattactg aagaatttat ttcggaattt
    10381 ttaaaagttt taaaatcttc taaaggaaaa attacattaa gtttatcgct agaagataag
    10441 aaaaacaaat cagaagttac tgttttttcg agaaatcacc aaataaaact aagcgacaaa
    10501 ctaatagaat atttggaaag caataaatat tttaactata tcaaacttaa ttgagtttat
    10561 ttatttttat taaaataatt taaacaagag ctaaaaaaaa ttgttaaagt ttaaatttaa
    10621 tattattttt agttttttat taaaacattt tcttatatta gcaacttaat ttaaatttaa
    10681 aagactatgg cattagaaat tactgaaaaa aattttgacg aattagtagt taataccgac
    10741 aagcccgttg ttttagattt ttgggcagaa tggtgtggac catgtagaat gattgcacct
    10801 ctaattgaag aaatgcacaa cgaatatggt gataaagctg ttattggaaa agttgatgtt
    10861 gacagcaaca atagtatagc agtaaaatat ggaattagaa atattccaac tattttattc
    10921 ttaaaagatg gagaagttgt tgacaaagtt gttggagcag ttccaaaagc cactttaaca
    10981 gcaaaattag acgcactttt ataaaataaa attgagagat aaattctctc tctgttataa
    11041 aaatgactat aaacgaaatt caagacgaaa ttatccaaga atttgaagtt ttcgacgatt
    11101 ggatggatag atatgcactt ttgattgaaa ttggaaaaga attagctgag atgccttctg
    11161 aatacaaaac agatcaaaac ttaattagtg gctgtcaatc aaatgtttgg tttcatgcag
    11221 aatttaaaga aggaaaaata tatttcactg ctgatagtga tgctgttatc acaaaaggaa
    11281 ttgctgcatt attaattaga gttttatcag aaagaactcc aaaagaaatt ttagaaacag
    11341 acttatattt tattaagaaa accggactac aacaacatct atctcctact cgttctaatg
    11401 gactactgtc aatgataaag caacttaaat tatatgcaat ggcatttaat gcattaaaat
    11461 aacgaattgt ggacaaaatt actgaaattg aaataaatat tgtcgaaaat ttaaaaacag
    11521 tttacgaccc tgaaattgca ataaatgttt acgatcttgg cttaatttat agaatagatg
    11581 taaacagcga ttatgatgta gatgttgaca tgacacttac tgcaccaaat tgcccaatgg
    11641 ctgacgacat aataaaaaat gttaacgatg ctataaaaaa cgttgaaaat gttagaagtg
    11701 taaacataaa tttagttttc gacccaccat ggtcaaaaga tatgctcagt gatgaagctt
    11761 tattagaatt aggtttatta taatttttta ataaaattaa aaaaagtatt ttaataatat
    11821 atttgtaaaa aattaaaact tatggctttt tttggattat ttggtaaaga aaaaaaagaa
    11881 aatttagacc aaggtttaga aaaaacccga agctctattt ttagcaaact caatagatta
    11941 atttttagta aatctaaaat agatgacgat gttcttgatg aattagaaga aatattaata
    12001 agttctgatg tcggagttga aacgactgtt agaattattg aaaaaattca agaaaatgta
    12061 aaaaaagaaa aatatcaaag ctctgaggag ttagatgaaa ttttacgtag tacaatagct
    12121 tcattattag aagaaaacga aaccaatcaa actgatgaag atgagatttt caactccgct
    12181 aaaccttatg taattatggt agttggagta aatggagtag gaaaaaccac aactatagga
    12241 aaattagctt ataaatttaa gaatgcaggt aaatctgtat atataggtgc agccgacact
    12301 tttagagccg cagctataga ccagcttcaa atttgggcag atagagttgg tgttccaatg
    12361 attagacaag atatgggttc tgatccagca tctgttgctt ttgatactct tagtgctgca
    12421 aaaaaagccg aagctgatgt agtaataatt gatacagcag gacgacttca taataaaatt
    12481 aacttaatga acgaattaac gaaaatcaaa agagttatgc aaaaagttat cgaagatgcc
    12541 ccacacgaag tgctactaat aattgacggt tcaacaggtc aaaacgctta cgaacaagca
    12601 aagcagttta caaaagcaac agaggttaca gcattagcag ttacaaaatt agacggaaca
    12661 gcaaaaggtg gagtagtaat tggaatatcc gaccagttta aaattcctgt aaaatatatt
    12721 ggaattggtg aaggaataga agatttgcaa atatttgaca aaaaagaatt tgttaattca
    12781 ttatttaaaa aatagattaa acctataatt accagcaagt tggatattaa aaattaaatg
    12841 ttaatataaa aaatgaaaaa aataattgta tattgaaaaa aaaaacttac ttttgaaaaa
    12901 ttattataat aggtaattaa tataaaatat aaataaaaat aaaaagggaa atatgaaaaa
    12961 atttggttta aaaatagttg tactaatcgc ttttgttagc ttaattaact attcgggctt
    13021 cagtcaaaac aattcaagta aagcttcagt tgaaacagct attgcaatta gcaaaaactt
    13081 tatgccatct gcgagcaaat ctggaattaa tgcagaaatt aaatcaattt ctagcataaa
    13141 aaacagtaat gaccaagaaa aaatacatgt tttaaacttt gctaatggtg gttttatgtt
    13201 ggttagtagt gataatagat ctgttcctgt tttagcgttt tctgatgaag gtgaatttaa
    13261 ttttaacgaa aacactgctc ctgcaacaaa aatgtggatt aatcaatatt taagacaatt
    13321 agattatatt gaagaaaaca atgttcaagc tacagaacaa atacaacaac tttgggcaga
    13381 tatgcttaat ggtaattacc aaagtagcac taaaaaagta gatcaattag tgttaacaaa
    13441 atggaatcaa gattggccat ataacatgtt tacaccagaa catccagaag gcaacaatgg
    13501 tcacacttat actggatgtg ttgctacagc aatggctcaa ataatgaaat attgggaata
    13561 tcctgatact gctaaaggtg ttgttaacta ccattggggc gattattacg aagttgattt
    13621 gaataaagaa tcttatgatt acagtttaat gccaaacttt tttatgccaa actctactca
    13681 acaacaaaaa gaagaagttg ctagactaat gttccactgt ggaatagctg ttcatatgga
    13741 ttatggacac gaaagttctg gttctcaaac agtttatgct tggcaagctt ttaaagaaaa
    13801 ctttaaatac agatcaggct catatttcac atataaagaa acaatgtctg atgcagaatg
    13861 gaaattcaga cttaaatacg acttagactt aggcagacca ataatgtatg ctggtgttag
    13921 tgaagaagaa ggtggtcatg ctttcatatg cgatggatat agagatacat ctttctttag
    13981 atttaactgg ggatggggag gttcaaaaga cggattctat tatttagaca aaattaatcc
    14041 acaaatggaa ttcccttttg cacaatctgc aattcttgaa cttgcacctc acggagctga
    14101 tttttgtaaa caaaacatag taatgacatt accttcttac tcatttgatg acggtagcgg
    14161 accaaactta tatgcaaaaa atacaaactg tagctggtta attaatccag atatgacaaa
    14221 tgcagacttt ttaagactaa cttttgacag attcgactta gcatctggtg atactttaaa
    14281 aatttggaaa ggtaacccaa taaacggtct aattgaacta gtaggagaat ttacttacaa
    14341 tcctggtgaa gtaattactg aaggcaataa aatttattta gaatttacta ctgacggaca
    14401 agctcaagct caaggttttc aagctcacta tgaagttgtt gtgctaggaa ttactgaaaa
    14461 tacaaatgct gttttaaaag tatatccaaa tccaacaact aatattttac aagtagaagg
    14521 acttcaaaca gaaaatattc aaatcatgga tatttcagga aaaatagtat taaatgctaa
    14581 tgtctctgaa aaatcaattg atgtttcttc tttaaaacca ggattatata ttattaaagc
    14641 aaataatcaa gttgctagat ttattaaaga ataataattg taaagttcaa atataaaaaa
    14701 atcggatatt taattcaata tccgattttt ttttttgtag ttatttgcta ctatcatctc
    14761 atcaaaaatt tatcacctgt attatctata agagttttct tccactcatc ggaatatcct
    14821 ggccaaataa ttttttttgg atagccccat ggatttcgta aaaactcacc attttcttcg
    14881 ggcttaacgt ttccatctaa atatttaacc aacagaaagt tattaaattc tttccaataa
    14941 tcaactaaag aatttgcggt attgcaagaa tattctgtta aaaattcagc tgctttttct
    15001 ggtgattgtt tatataattt tagagccgtc tcatcaataa tattcacata ttcaacatat
    15061 ttattttcta aaaatgactg atgtttttca atttctggca ttactctatt gtaaaataaa
    15121 tatttaaaat gtgctaatct attaaacacc caaaaagctg catcgtcttt ataagttaaa
    15181 atatctccat atccttcagc ccatttttcg gcaggttttg ttattccaac ataaaaagga
    15241 gtatatacac atgaacccgc atcatcaaca cctaaccaat aaatccctcc aatttcgttt
    15301 ggtaaccacg aacgcatttg agctacaaaa gaaaaacctg tttgttgagt tacagttact
    15361 ctttcattca aatattcttt tccttcatat ttaaaagtta aaggtcgcca acgataaggc
    15421 atacccgaag gctctgctcc tatgtctttt gtcatatcta attctgtgcc ttgcatataa
    15481 tttctaaaaa atttgaataa gtctaattga cttaatttgc gatttggctt aatatataat
    15541 ggaagcctat tctccaaatc gttaccgcag gctatatccc aatattttgc catatcatca
    15601 cttatttcgt taaacatagc ccagactcta agttcgcaac ctcttgctgc actataatta
    15661 ataggtgcat aagcttgtga aaatgaaaaa tcttcatctt ttcctgaaaa atatttttta
    15721 tctctagcaa aactaattac atcatgagaa taaactactt caatatgttc gtcaaagatt
    15781 ttatctaaat ttttacatga aatagaattt ttcatatttt ccaacggaaa gtttgtaatt
    15841 ctagaatgat ttgcatgagc tgaaacataa ccttctggaa tttttattgc aacacaaaca
    15901 attcctttat ttttattcac atattttttt tgttttttat catatacctg ctccactcct
    15961 tttccaacaa gttctacaat ccacacttca ttaggatcag caattgaaaa agtttcacca
    16021 ctacttgcat aaccatattc attaaataat tctatcatta ttttaattgc ttctctggca
    16081 gtttttgctc tttctaaagc aataaacatc atactaccat aatcaattat tccagtagta
    16141 tcaactaatt catcacgacc gccgaaagta gtttcaccaa ttgcaagttg atgttcattc
    16201 ataaaaccta caactttata agtatgaggc acttgtggaa tttttcctaa aggtttattt
    16261 gtacctctat cataaattat acgcatattt cctttttccc aatcggctgc tggtcgccaa
    16321 taaagttcac cataacgaat atgagagtcg gctgcataag aaatcattgt gcttccatca
    16381 acagaagcag tttttgttac caaataattt gtacaagcat cactaaaaaa aggagtgaaa
    16441 ataaaaacta aaactattaa aagataattt ttcataaaaa taaatttaag tttacgaata
    16501 taacaaaatt aaaactaaaa caacaaattg tttcttataa atattataga atctgatttt
    16561 ttaatctcca ttcaagaaat ttaaaaatta gctattattt gaataaaaat tcttatcttt
    16621 gtgtaaatta aaaaaactat aggagttgcc aaaaacccaa aaagtggagg catattaaaa
    16681 attatttaat tatgagaaaa attttgttat ttaccttagc ctttagcatt ttttttgtag
    16741 gctgcaaaaa agaaggagtt tataacccta ataaaaaaat taaacgaatt tattacgaat
    16801 atggtttaaa tactggcaca aaaacccttt cgcaagaatg gacttgggaa aaagacaagt
    16861 tagttaaaat taaacattct ttctatactg agatattatc ttatgataat aaaaatcgac
    16921 tgataaaaat tcaagatttt gattttaatg atagatacga aattatatac gataaaaaac
    16981 taataagtaa gattgaatat tacataggca attcgttatg tcaaacaatg aattttaagt
    17041 atgataaaga taaaattgtt gaaattacaa ttaagaatat tgataataac aaaatgcttg
    17101 aaaataaatc aattaaacca gatagtttta tttcaacaat tttaccattt aagataccag
    17161 ataaagctat gcgaactaaa tctgctatct cgtcaacata tattataact tatgaatatg
    17221 atggagataa tataagtaaa acaaaatata ttgacgacga taatcaagtt cacgaatata
    17281 ctcaagaaaa ttatgataac aaacttaatc cattttataa tagccgtttt ttatcaaatg
    17341 gtttaggcat tggcttttca aaaaataatg ttggaaaagc aacaactata acttcagaca
    17401 atgatgtaaa gacaatagag tttgaatacg tttatgatgg aaaatttcct acagaagtta
    17461 tagaaaaaga atcatcgtct gcaactaatg aatttagtat taataaaaca tattatgaat
    17521 atcaataaat atattatttc aagaatatag taacataaaa gataaaagga gttgccgaaa
    17581 gcctaaaaag tggaggcata tttaaaaatt ataaattatg agaaaaattt tgttatttac
    17641 tttagcctta agcatttttt ttgtaggctg caaaaaagaa ggagtttata atcctaaaga
    17701 aaaaatcaaa cgagtttatt atgaacgtgg cttagatgct agtacaaaaa gacttgtaga
    17761 agaatggact tgggagaaaa acaatttagt taaaattgat tattatgatg ataactatgg
    17821 tattataaaa attgactata ctgaaatttt atcttacgat gataaaaatc gactaacaag
    17881 aattcaagat tttgttttta atgaaagata tgaaattaca tatgataaaa aacttataag
    17941 taagattgaa ctttacatag ataatgaatt ataccagatt atgaatttta agtatgacag
    18001 aaataaaatt gttgaagttt ctgtaataga gattgataat tccaaaatcc tcgtgagcaa
    18061 atcaaataaa ccacaaagtt atatttctaa aattttacca tatgaaatac ctgaaaacac
    18121 tatgcgaact aaagctgcta gtttatcata tatttacact tttgaatatg atggagataa
    18181 tctaagtaaa gtaaaatata ttgacgatga caatagagta agagaatata attatgaaaa
    18241 ttatgataaa tataaaaacc cattttattc tagccgtttt atatcaaata atatagaaag
    18301 cggtttttca aaaaataatg ttggaaaaat tacaactaca actataggaa atactgttag
    18361 aacagcagaa tttgaatatg tttatgacgg aaagtttccg atagaaatta ttgaaagaac
    18421 aacttattct ggtagtgatt taatttggat ttataaaaca tattatgagt accaataaat
    18481 aaattgcaat aactcgatag aaataaaaaa actgcctcta ttcaaggcag tttttttgta
    18541 atattctcat atagttaata ttttgcttaa ctatatggtt ttattattta gtcttatttt
    18601 tttctatagg taatactccg cctgccattt cgccaataat attaactaaa tcgctacctt
    18661 caggaattac aatttttgaa ttatctttaa gagccttttc aattgtttct aattttttca
    18721 acagttgtgc atttccaacg aagtattggt ctgcagcttc gttaaccaat ttgattgctg
    18781 aagcttcacc ttctgcatat aaaatttttg cttgttttac accttcagct tctttaattt
    18841 ttgcacgttt tgttccgtca gcagaaattt cagcagctgt agcaaagtcg atagcagcaa
    18901 ttttttcgtt ttcagctttt acaactttgt tcattgtttc ttgaacatca tgtggagggt
    18961 cgatttgttt taactctgtt ctaacgatat caattcccca agaacgagtt tcattacata
    19021 aagttttgtg tagttccgca tttatttttc ctctttcact atttgcagct tttaaagtaa
    19081 gagtaccaat aatatttctt aatgttgtac gagcaaggtt aacaatttgc cattgataat
    19141 tatttacatt gtattgagaa tttttaacgc tttcttcatc aaaatttact ttaaaataaa
    19201 cttgagcgtc aacacttgcg ttaagattat catttgtaat aatttcttgt ggctgtgcat
    19261 caaccatttg ctctgtaata tttactctat acattttatc aatcacagga ataatccaat
    19321 gaaatcctgg ttttgcaaat tttctatatt ttcctaatct ttcgataagt ccgcgatgag
    19381 ttggtctaac tatttttatt cctgcaaaga aaatcaacag tacaatagca ccaataataa
    19441 taataagttg agtgtccata attttaaaat ttaattgttt ataatcctac aaaaaagtta
    19501 aaaaagtttt tttattattt aagtgtcccc ttttattagg acagtttttt taatttgtta
    19561 atattttatt t
//
LOCUS       DAOPNX010000043        19391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_3441_length_19391_cov_6.343918, whole genome shotgun sequence.
ACCESSION   DAOPNX010000043 DAOPNX010000000
VERSION     DAOPNX010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19391
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3441_length_19391_cov_6.343918"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="PKN53_06225"
     CDS             <1..1372
                     /locus_tag="PKN53_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOD88580.1"
                     /translation="LFSKYFNVFFSFLHRTHVVISLILLISLNLFSQESSETNSPATN
                     AKTGWTFGLVPVVSYNSDLGFQYGGLVNFYNYGDGSSFPKYKHSIYAEVSRYTKGSGI
                     YRLFYDSEFLIPKIRLTADLSYLPDQALDFYGFNGYDAVYNPNFQNESSTEYITRMYY
                     KHQRDILRLKLDFQGATGAKNLRWVAGYELMDMQIGSVPIDKLNKGKDPEDMLPNVPN
                     LFDNYVNWGIINQNEKDGGWHNSIKLGTVYDTRDNEACPMKGIWSELVIFNSFSKNFT
                     FGKIALTHRQYFTIIPNDFSFAYRLAYQANVYGKSPFYMLPYMVYSYMPSSSIDGLGG
                     SKTIRGLIRNRVVGDGVVYGNLEFRYKFVRFKFIKQNWYIALNPFLDAGMVVQKTKID
                     KTGIPNIVDQSLFFKDNAESVHLTYGCGLHIALNENFVIAADIGLPVKKEDGKMGIYI
                     GMNWLF"
     gene            complement(1771..3120)
                     /locus_tag="PKN53_06230"
     CDS             complement(1771..3120)
                     /locus_tag="PKN53_06230"
                     /inference="COORDINATES: protein motif:HMM:NF016283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HOD88581.1"
                     /translation="MAKGRILQVKQSEITIISQQNTDYISLTDMTVTFREGSGLISKW
                     ITNKNTIEYLGVWERINNPNFNYPEFGVIGQEAGTNRFIMSVGQWVARTNAIGMLVKA
                     GRYGGTYAHKDIAFHFAMWLSPEFQIYLVNEFQRLKDEENNRLKLEWSLQRTLVILNL
                     QFNPLQMGESELIISDFLYNTYSITDIQNGTVTIIGKYGDIDANDYVQAYDAALALQY
                     SVGLDPLPLTDPLPWENWRVIIGNVDGVGDITANDASLILQHSASIISTFPVEGAKSL
                     EEPIAEITITQDNDELVFTSSGDLYGLNIYSTNGTFVTMNTPTVLDQNMLSVFNINET
                     NYNVGLCTAYSPSDNTEILRIPLECTDNEELIFNMIVNKSAITKSFTVDCSLLGISES
                     IEEKLNVYPNPANDKIEIEGLQAGQIEIINLQGQIIKKINLTDEKTTIDISELSVGL"
     gene            complement(3152..3493)
                     /locus_tag="PKN53_06235"
     CDS             complement(3152..3493)
                     /locus_tag="PKN53_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOD88582.1"
                     /translation="MNYFCAKSKNHKQSRNLFVREVKKLYRREFIRWYKMTADINSIL
                     KMFRNLELPIPTLYIMGDEDEMFLPAIRQVVKEHVNHSQLAVIPNCGHVVNVEKPDVF
                     NRMVCKFIDSI"
     gene            complement(3510..4886)
                     /gene="mnmE"
                     /locus_tag="PKN53_06240"
     CDS             complement(3510..4886)
                     /gene="mnmE"
                     /locus_tag="PKN53_06240"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOD88583.1"
                     /translation="MKDLFNNTVCAISSPPGVGAISVIRLTGEKSHQILTNNISSKSK
                     YENLKAGKLLFTKILADGNILDEVLLVKFDAPNSFTGENMLEIYCHGSDFIQQEILHL
                     LVKNGANIALPGEFTKRAFINGKMDLSQSEAVADIISSNSSESHRLAINQLKGGISNE
                     IALLREKMIELISLMELELDFSEEDVEFADRSRLLQIIQEIKTKINLLIESFKYGNAV
                     KNGVPVAIVGKPNTGKSTLLNTLLKEDRAIVSDIPGTTRDSIEEEIFINGIKFRFIDT
                     AGLRESKDKIEEIGVKRSLEKINLSQVILLIIEANSSEKEIDTIIKQVEKEINSSQKL
                     ILVFNKIDLLNKENIDLKYNYESIKISAKHNKNIDLLHNSLSFYVKSLKTGIGDVILT
                     NARHIEVLHKIDLALDRAIEGLNMQLSSDLVSQDLREAMHYLGEITGQVSNEEVLGAI
                     FSKFCIGK"
     gene            complement(4883..7090)
                     /gene="selD"
                     /locus_tag="PKN53_06245"
     CDS             complement(4883..7090)
                     /gene="selD"
                     /locus_tag="PKN53_06245"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOD88584.1"
                     /translation="MGIYLDYNATTPIDKEVAEFMKPFLNDYFGNPSSSHSFGVNAKK
                     AVENARAKIANLLGCKASEIVFTSGGSEANNYAIKGYAFANENRGKHIITSQIEHPAV
                     IEVCKFLETKGFEISYIPVDEFGIIDLKKLESEIRSDTILISVMHANNEIGSIQPLKE
                     ISKIAKKYNIVFHTDAAQSVGKIPTKVNELGVDMLSLAGHKLYAPKGIGVLYIKEGIF
                     LEKLIHGAEHEQNKRAGTENVLEIAGLGKACEIAERDMEKNIAHSLKMRDLFHTLLSE
                     KIESIKLNGHRELRLPNTLNISFKNIEANILLNEMETHGIAASAGAACHAEAVKVSTV
                     LQAINVEPDYAMGTIRFSVGRQTTENEIREAAKIIIEIVENLKSNTDKSVEPTENPKI
                     FKLTKYTQGLGCACKLSPQDLEKVLKDLPSVFDKNVLIDASNSDDAAVYKIDENTALV
                     QTLDFFTPVVDDAYDFGAIAAANALSDIYAMGAKPIFALNIVGFPANRLDISVLNEIL
                     RGAADKAKEAGISILGGHTIHDVEPKFGMAVSGLANPNKIKTNAGAKHGDAIILTKKI
                     GTGILTTAIKKDLISEKSKHEVIASMKELNMQSAEIISKYEVNACTDITGFGLAGHLS
                     ELLLASKLSAEIFENEIPYFEQTEELAYAGVVPGGSLNNLQHFSKNIIWKIQISETRK
                     LMFCDAQTSGGLLFTLPNELKDKVIDDLHKNGITTAKYIGNCYKDGESKIIVK"
     gene            complement(7152..8576)
                     /locus_tag="PKN53_06250"
     CDS             complement(7152..8576)
                     /locus_tag="PKN53_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOD88585.1"
                     /translation="MDRSQRLGTEKIKPLLWSLSLPAMIGMLSVTIYNVADTIFIGHA
                     VGTLGIAAISVSLPLMMSITTFGQAIGLGGASVISRALGAGNKAKAQLTLHSLLTIII
                     SINFILITLAYLKAGFFLKLFGANDEILPYAVQYVRWSLPGSFFLNILFVSVHQIRAE
                     GNARFPMFSQILGGVLNLIFDPIFIFGLGWGLMGAALASSISQFVGFCLAMWYFTKNP
                     KSVLKLNIRKLWALPDKAITLEVLAIGASSFGRQIAGSIMSIVLNNALLRYGGAASIA
                     AFGIINRITMFVFMPLFGLNQGFMPIAGFNYGAKKILRVLRGMKIAIIWSTSFCIATL
                     ILFFVFANSIVSVFTTDKELIAMATKSLRIFILAFPVVGFQIVGSGLFQALGKAKASM
                     FLALARQVLFLIPFVLIFPKFWGENGIWFSFPISDFLAASVTFLMVVNLVKKLKKEDK
                     ENRRLNLEKAKTKPKFNLQDETVL"
     gene            complement(8609..10555)
                     /locus_tag="PKN53_06255"
     CDS             complement(8609..10555)
                     /locus_tag="PKN53_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOD88586.1"
                     /translation="MKRIFSFLIIFFAFSFFVQAQNNSVYTKNKSAIKSFNKAVSYME
                     QRQNRMAITEIEKAIEKDPNFLEAWLLKAEIYDFLGKANESYLSYAKVFEIDAQYDPA
                     LSFKLAANSYKIGKYSVAKKYIDFFCNEVDTMRYRNYDFKRLREFIYFADSAYKNPKD
                     FKPINIGPGVNTHFDEYWPSLSVDERVLVFTRQIPINPNSTSRDPSSMQEDLFVSYFD
                     DKTGQFIKAIPMPENINTKLNEGAQCISADGKTCVFTACNRPEGKGSCDLYIMFLRGG
                     KWTDPQNLESINTASWDSNPSLSADGKYLYFASGRAGGYGKTDIWRVRIDAEGNALDE
                     AENLGPQINTQFEELSPFIHPDGRTLYFASRGHPGMGDFDLFYSKLDNNGNWSKPVNL
                     GYPLNTMGEERSLIVNARGDIAMFASNVGRKDLDIYFFLIPEEIKPVAATYVKGYVYN
                     AKTNQRLEAKCELFDIESGEEMVRLTTDKITGEYLVTLPVERDYAFNVEKTGFLFHSE
                     NFSLKNIKDPEQPFILNIPLQPLEEGVTVILKNIFFAFDSYELLPESYAELKIVVDYL
                     NTNPNLKIEIGGHTDNVGSKAYNKTLSENRAKSVYTYLISKGIDKDRLSYKGYDFSIP
                     IADNATEEGRALNRRTEFKVISNK"
     gene            complement(10575..11204)
                     /locus_tag="PKN53_06260"
     CDS             complement(10575..11204)
                     /locus_tag="PKN53_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOD88587.1"
                     /translation="MNKELKDIVKAGKIPLLEEFYSIQGEGFNTGKAAYFIRTGGCDL
                     ACHWCDSKETWKPEIHEYIEIDAILQRVKQTPANTIVITGGEPLIHNFDEFCAKAKSQ
                     AFTLMLETCGAHNFSGVWDWVCLSPKQTKPPKAEYFQIADELKVVIFEEKDFLWAEEC
                     AKKVTNSCALFLQPEWSRFEKIGIMLVDYVKQNPKWNVSLQTHKFLRIP"
     gene            complement(11204..12436)
                     /locus_tag="PKN53_06265"
     CDS             complement(11204..12436)
                     /locus_tag="PKN53_06265"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOD88588.1"
                     /translation="MMRNTYLFLIFLMFSAQLFAQNTDKASYKTYPSGFYFKQILRDN
                     LGDEAWAKRKYQHREVFKMEMQDRIFPTDLTKYTTVYHLQPVSQGNSGTCWAYSAISF
                     LESEVYRMTSQKIKLSEIYIAYWEYVERAEDFIKTKGETFLGEGSESNAVLRIMKNHG
                     LMPLSAYSGLKDQQNFNNHKALFEQYETYLNFQKQQNSWNKTEIMDSIYKILDYWIGT
                     PPDNFNFEGKNYTPQTFMSEKLKLKPNNYFSFMSTKEIAYNQKGELVENDNWWHCKDY
                     YNISLNDFMSLINTSIENRYSISICGDVSEPGICQIEEVAIIPDFDIPSAYINEDARQ
                     MRLISGATTDDHCVHLVGYQIINGEYWYLIKDSGAGAFDGKNKGYRFFHEDYVKLKMM
                     NILLNVEPARSVLDKIIK"
     gene            complement(12751..15126)
                     /locus_tag="PKN53_06270"
     CDS             complement(12751..15126)
                     /locus_tag="PKN53_06270"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOD88589.1"
                     /translation="MLKVQFVILFTLSVLLANSQVTVSGYVYDAETNEAIIGANIVDS
                     VNSNGTSTNSQAYYSIKTNSTELKVSFIGYETQIIKINQSQNNIQNIYLKPLAYQLDE
                     VVIRVESDKTVNVAKLELKEIEQIPSLTAQPDVVKSTQILPGIQSQSEASSLLVVRGG
                     DPGQNLFILDNTPLIYVNHLGGFMSVFNPDIINNITIYKSGFPAKFGGKLSSIIDITQ
                     RSGSANGFKGSFGIGLTDVNLSLEGKFTDKITYIFTTRKTLFDVLMIAASGLSDGGDY
                     FISYGFHDVNFKSVWKINSLNTISMNLFQGDDYLKIWNKKDDLDLFNSKIKANNIWGS
                     LMGAVSWNSIISPKIFIKTNISYSGYRVKDLQSFNNSINQGFKNKFESKVQEISLNSV
                     WSFNLMRNWTLIGGLHLSHFIYTPYDYRVLGKENYYEVLSEEINNSSISLSNKFKFFK
                     FIETELGARVGNYFLSNSKEFFIEPRFDINFNFNKLGVINFNYMQVSQASHLLISSGN
                     IYVNEIWIPSCKDIPVSSSLQKSLSWSKGFYDNMFSLNIGVYDKELYNLITSEKNVYY
                     LSELENWKQSIETGGVGKVYGLEFMFKKNCGKWTGLISYHLSKSTRKFHNINDGKEYL
                     YEYNRPHDVSIFINRKINKKLSLSLVWNYQSGLPITPVLGRQNVVDPHTNEIYEAFIY
                     GERNSSKMKDYHRLDIGLKYETISKKRKLKTVWTFSIYNAYNRQNPYLYYYNNNSSPE
                     IHRPENGFAKLNLYQISFFPILPSVSYKVYFGKEDINFKGFSLKKWLNFEE"
     gene            complement(15129..15962)
                     /locus_tag="PKN53_06275"
     CDS             complement(15129..15962)
                     /locus_tag="PKN53_06275"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HOD88590.1"
                     /translation="MQKTLLIFAFLIILFSSSCRKLVQDEFEDFNSKITINSIIVQDE
                     QIHLHVSLTSGLDAYELSNANNANIEMYNQDSVLLNFNYVEKGHYISDYIAKENDEFY
                     LKVNVNNSIVSSSCFIPDRVNISEFHIIENAWVDNEGVLQPKINFSIINDLSKDLFFE
                     AYIMIYDENVTEVIEEYPVLFFDNFELEKDIISKSVKISHHFYSNNINNYKYQLIIKS
                     VDSNYYKYVKSFENYNLSRFPDFSNAGIVPTNLFSNINNGYGIFCGYSQTLSDIKAPI
                     D"
     gene            complement(15967..16506)
                     /locus_tag="PKN53_06280"
     CDS             complement(15967..16506)
                     /locus_tag="PKN53_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88591.1"
                     /translation="MRLRIFLILILFPFLVNAQKSSYINKRLTGDVSFTRMNYSLVPE
                     YKQVEKGVFVSLNYGLAKFLETGVYYNRYFASSFVSLNFVGIQNRFHILPFFIESNNR
                     FFRADAYVLNQTGLFLYKTTNSGFSNYFYTNLGAGLSLFVFKNLGLNFDYTWSFVFSK
                     NVVKSNFQNGFKLGLILKF"
     gene            complement(16619..17032)
                     /locus_tag="PKN53_06285"
     CDS             complement(16619..17032)
                     /locus_tag="PKN53_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HOD88592.1"
                     /translation="MEILYTAKVTTSGGRNGHVKSSDGIIDMDVRMPKSLGGQNDDYP
                     NPELFFGAAYSACYDGALNLVARMERFKITSTTTANVSIGKVEDGSLAISVVLDIDVQ
                     GVDKETAQKLIEKAHQVCPYSKAIRNNVNVVLNLL"
     gene            complement(17470..18048)
                     /locus_tag="PKN53_06290"
     CDS             complement(17470..18048)
                     /locus_tag="PKN53_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88593.1"
                     /translation="MKFRIFLFLIFISSLTSCSINGSFQGLFSYYKKTKKEGKINFLK
                     FDTNNDYLNSYSDSTTIVLSNGKEIKKYLKNFEKSIIYIWSPKCKSRFCYSLNLLQEI
                     CSQKGIELFVVAEYYDSKYMSKNYKISHLIIGIDTQYYKTNLTSKYLSKFIKDLIDEV
                     DDTTFKEIYNAKFFSFRNGKFEKSFDSIEDIK"
     gene            18195..18761
                     /locus_tag="PKN53_06295"
     CDS             18195..18761
                     /locus_tag="PKN53_06295"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOD88594.1"
                     /translation="MEFNYYAYIILPLLIFLARICDVTIGTVRIILVARGHKILAPIL
                     GFFEILIWIMAISQIMGNLDNWVSYLAYALGFATGNYIGMLVEEKIAMGIVGLRLVTN
                     KPATQLIEALKENGYGHTHMDANGATGPVNVVFITVNRKNLNELIDLINVYNKGAFYT
                     VEDIKLVNKGIFMQNNASTRKLFQRKGK"
     gene            18765..19364
                     /locus_tag="PKN53_06300"
     CDS             18765..19364
                     /locus_tag="PKN53_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOD88595.1"
                     /translation="MKFEELNIAQSLKDGIDSFNYQELTPIQEKTLPIILQNKDLLAC
                     SQTGSGKTAAFLIPTMNKILNSERNGIKALIVAPTREICIQIEEQIMGLGYFAGISSV
                     AVYGGSNQEDFTIQKNALENKTDIIVATPGRLLAHLNLGYVKLNKLVTLILDEADEML
                     DMGFYTDIMNIISYLPKNRQNLLFSATMPSSICKVSPRC"
BASE COUNT         6516 a         3270 c         2641 g         6964 t
ORIGIN      
        1 gctattttca aaatatttta atgttttttt tagtttctta catcgaactc acgttgttat
       61 tagtttaatt ttacttattt ccttaaattt attttcacaa gaaagtagtg aaactaacag
      121 ccctgctact aacgctaaaa ctggttggac ttttggactt gtccctgtag tctcctacaa
      181 ttcagattta ggctttcaat atggtggact tgtaaatttt tacaattatg gcgatggctc
      241 tagttttccg aaatacaaac attcgattta tgccgaagtt agtagatata ccaaaggttc
      301 tggaatttac agattgtttt atgattctga atttctaatt ccaaaaataa gattaacagc
      361 agatttatca tatttgcccg accaagctct tgatttttat ggttttaatg gttatgatgc
      421 tgtttataat ccaaattttc aaaacgaaag ttctactgaa tatattacca gaatgtatta
      481 caaacatcaa agagatattt tgcgattaaa acttgatttt caaggtgcta caggtgctaa
      541 aaatttaaga tgggttgcgg gttatgagtt aatggatatg caaataggca gtgttccaat
      601 tgataagtta aataaaggca aagacccaga agatatgctt ccaaatgtgc ctaatttatt
      661 tgataattat gttaattggg gaattataaa tcagaatgaa aaagatggtg gctggcacaa
      721 tagcataaaa ttgggtactg tatatgacac tcgtgataat gaagcttgtc caatgaaagg
      781 tatttggtct gagcttgtta tttttaactc atttagcaaa aatttcactt tcggtaaaat
      841 tgcacttaca catcgtcaat attttacaat tattcctaac gatttttctt ttgcttaccg
      901 acttgcctat caagcaaatg tttatggaaa atcaccattt tatatgctac catacatggt
      961 ttattcttat atgccttcat caagtataga tggtttaggt ggcagtaaaa ccattagagg
     1021 acttattaga aatcgtgttg taggtgatgg tgttgtttat ggaaatcttg aatttagata
     1081 taaatttgta agatttaagt ttataaaaca aaattggtat atagctttaa atccattttt
     1141 agatgctgga atggtagttc aaaagacaaa aatcgacaaa actggaattc ctaatattgt
     1201 tgaccaatct ttatttttta aagataatgc cgaaagcgta catttaactt atggttgcgg
     1261 tctgcacatc gctctaaacg aaaactttgt aattgctgct gatattggct tgccagttaa
     1321 gaaggaagat gggaagatgg gtatttatat tggtatgaat tggctgtttt aaagttgttt
     1381 tataagcgtc caactgctac gttgctttcg atattcggac tcagtcacgt acacttcggc
     1441 tttgctcagt gcaacgcttc atgtacgctc ctttgtcctc aatctcagcc gccttgcatt
     1501 tgaacacttc taaaacactt taactactat gaattgagtt aaattaattg ataattaaga
     1561 attatgagct cgctacgctc gcttggggtt tgcttcgcag agtgagcttg cgaactttgc
     1621 ttgctcggat ttataaaacc aaatgaacgt ggagagcgta ggataataaa aaacgaaaag
     1681 gcttcatatc aactctaaag ttttttacga agccttatca tagagcttga aactaagcct
     1741 ttgttaggct ttaaaagtca ttgtgttata ttatagccca acggataatt ctgatatatc
     1801 tattgttgtt ttttcatctg tcagatttat ttttttgata atctgtcctt gaagatttat
     1861 tatctctatt tgtccagctt gcaaaccttc gatttcgatt ttatcgtttg caggatttgg
     1921 atatacattt aatttctctt ctattgactc actaattcct aaaagactac aatcaactgt
     1981 aaatgacttt gttatggcac ttttattcac tatcatgttg aaaataagtt cttcattgtc
     2041 agtacattct aaaggtattc tcaatatttc tgtgttgtca gatggcgaat aagctgtaca
     2101 caagccaaca ttataatttg tttcattaat attgaataca ctcaacatat tttggtctaa
     2161 tactgttgga gtgttcattg ttacaaatgt tccgttagtt gagtaaatat ttagtccgta
     2221 caaatcacca gatgaagtaa acacaagttc gtcattgtct tgtgtaattg taatctcagc
     2281 aattggttct tccagtgatt ttgctccttc gacaggaaat gttgaaatta tacttgctga
     2341 atgttgcaat atcaatgatg catcatttgc agttatatct ccaacaccgt caacatttcc
     2401 aataattact ctccagtttt cccaaggtag cgggtcggtt aatggcaatg ggtcaagtcc
     2461 aacggaatat tgcaatgcca aagctgcatc ataagcctgt acataatcgt ttgcatcaat
     2521 atctccgtac tttccaatta ttgttactgt tccgttttgt atgtcagtga ttgaataggt
     2581 gttgtataga aaatccgaaa ttatcagctc cgattctccc atttgaagcg gattaaattg
     2641 aagatttaaa attacaaggg tgcgttgtaa actccactct aatttcagac ggttattttc
     2701 ttcatcttta agtcgttgaa attcattaac caagtatatt tgaaattctg gacttaacca
     2761 cattgcaaag tgaaaagcga tatctttatg tgcatacgtt ccaccgtatc gtcctgcttt
     2821 tacgagcatg cctatagcat tagtacgggc aacccattga ccaactgaca ttataaatct
     2881 attcgtacca gcttcttgcc caattacccc gaattcgggg taattaaaat tgggattatt
     2941 tattctttcc caaacgccta aatattctat tgtgttttta ttggttatcc atttgcttat
     3001 caatccacta ccttctcgaa aagtaacggt catatctgta aggctaatat aatcagtatt
     3061 ctgttgactt ataatcgtta tttcggattg ttttacttgt agtattcttc cttttgccat
     3121 aaaaagtaat ttaaacttgc ttctttttaa tttaaatcga atctataaac ttacaaacca
     3181 ttctattaaa aacatcaggt ttttcaacat ttacaacatg tccacagttt ggaattactg
     3241 ccaattgtga gtgattaaca tgttctttca cgacttggcg aatggcaggt aaaaacattt
     3301 cgtcttcgtc gcccataata tataaagttg gaataggcaa ttcaagattt ctaaacattt
     3361 ttaatataga atttatatcc gcagtcattt tataccatct tataaattct cttctataca
     3421 gttttttaac ctctctaaca aaaagatttc tcgattgctt atgatttttg ctttttgcac
     3481 aaaaataatt tattttttca aaataacaat cacttcccta tacaaaactt agaaaaaatt
     3541 gctcctaaaa cttcttcatt agaaacttga ccagtaattt cgcccaaata atgcatggct
     3601 tcacgcaaat cttgagaaac taaatcactg cttaactgca tatttaatcc ttctattgcc
     3661 ctgtcgagtg ctaaatcaat cttatgcaaa acctcaatat gacgagcatt ggtcaaaatt
     3721 acatcgccaa tacctgtttt taaagatttt acataaaaac ttaatgaatt gtgaagcaaa
     3781 tcaatgtttt tattatgctt tgccgaaatt ttaatgcttt cgtaattgta ttttaagtca
     3841 atattttctt tattgagtaa atcaattttg ttaaaaacta aaataagttt ttgagaagaa
     3901 ttaatttctt tctctacttg ttttattatt gtatctattt ctttttcaga tgaattagct
     3961 tcaattatta aaagaataac ttgagataaa tttatttttt ctaaactacg tttaactcct
     4021 atttcttcaa tcttatcttt actttctcgc agtccagctg tatcaataaa cctaaattta
     4081 attccattaa taaaaatttc ttcttcaatc gaatctcttg ttgttcctgg aatatctgaa
     4141 actattgccc tatcctcttt taacaaagta tttagcaagg ttgatttccc tgtatttggc
     4201 tttccaacta ttgcaacagg aactccattt tttacagcat ttccatattt aaaagattct
     4261 atcaataaat ttattttagt tttaatctct tgaattattt gcaaaagcct tgacctgtct
     4321 gcaaactcta catcttcttc gctaaaatca agttctaatt ccattaaaga aattaattct
     4381 atcatttttt cacgaagcaa ggcaatttca tttgatattc caccttttaa ttgatttatt
     4441 gctaatctgt gcgactctga agaattactt gaaataatat ctgcaactgc ttctgattga
     4501 gataaatcca ttttaccatt tataaaagct ctttttgtaa attcacccgg caaagcaata
     4561 tttgctccat ttttaacaag caaatgtaaa atctcttgtt gtatgaaatc cgagccatga
     4621 cagtatattt caagcatatt ttcgcctgta aatgaattag gtgcatcaaa cttcacaagc
     4681 aaaacttcgt ctaaaatatt gccatcggct aaaatcttgg taaacagcaa cttacctgct
     4741 tttagatttt catatttgct ttttgaactt atattatttg ttagtatctg atgagatttt
     4801 tccccagtta aacgaattac agaaattgct ccaactcctg gcggagaaga aattgcacaa
     4861 acagtattat taaataaatc tttcatttta caataatttt actttctcca tctttgtaac
     4921 aatttccaat atattttgct gtagtaattc catttttgtg taaatcatct attactttat
     4981 cttttaattc gtttggaagc gtaaacaaca gtccaccaga tgtttgagca tcacaaaaca
     5041 taagttttct tgtttcagaa atttgtattt tccaaataat atttttagaa aaatgctgta
     5101 aattattcaa acttcctccc ggaactacac ctgcataagc taattcttca gtttgttcaa
     5161 aataaggaat ttcgttttca aaaatttctg cactaagttt actcgccaac aataattcgc
     5221 ttaaatgtcc agccaagcca aaaccagtaa tatcagtaca agcgttcact tcatattttg
     5281 aaattatttc agcactttgc atattcaatt ctttcattga agcaattact tcatgcttag
     5341 atttttcaga aattaaatct tttttaattg cagtagttaa aattcctgtg cctatttttt
     5401 ttgttaagat aattgcatcg ccatgctttg ctcctgcgtt tgttttaatt ttattaggat
     5461 ttgcaagacc agaaaccgcc attccgaatt ttggttcaac atcatgtatt gtatgacctc
     5521 ccaaaattga aattccagct tctttggctt tatctgctgc tcctcgtaga atttcattta
     5581 aaacagaaat atccaatcta ttagcaggaa aaccaacaat atttaaagca aaaataggct
     5641 tagctcccat tgcataaata tcgcttaaag cattagccgc agcaattgca ccaaaatcat
     5701 aagcatcatc aactacaggt gtaaaaaaat ctaaagtctg aactaaagca gtattttcat
     5761 ctattttgta aactgctgca tcatcagagt ttgaagcatc aatcaaaaca tttttatcaa
     5821 aaactgaagg taaatctttt aaaacctttt ctaaatcctg cggacttaat ttgcaagcac
     5881 aaccaagtcc ttgagtgtat ttagttagtt taaatatttt aggattttct gttggctcaa
     5941 cacttttatc agtatttgat tttaaatttt caacaatttc tatgataatt tttgctgctt
     6001 cacgaatttc gttctcagtt gtttgtctgc caaccgaaaa gcgaattgtt cccatagcat
     6061 aatcaggctc tacattgata gcttgtaaaa ctgtagaaac ttttactgct tctgcatgac
     6121 aagccgcacc agccgaagca gctattccat gggtttccat ttcgttaagt aaaatatttg
     6181 cttcaatatt tttaaaacta atatttaaag tgtttggcaa tcgcaattct cgatgtccgt
     6241 tgagttttat actttcaatt ttttcgctaa gcaaagtatg aaataaatca cgcattttaa
     6301 gagaatgtgc aatatttttc tccatatctc tttctgcaat ttcacaagct ttgcctaaac
     6361 ctgcaatttc taaaacattt tctgttcctg cacgcttatt ttgctcatgc tcagcaccat
     6421 gaattaattt ttcaagaaaa attccttctt ttatgtataa aactccaatc ccctttggtg
     6481 cataaagttt gtgtcctgct aaacttaaca tatcaacacc taactcatta actttagttg
     6541 gaatttttcc aacagattga gcagcgtctg tatgaaaaac tatattgtat ttttttgcaa
     6601 tttttgaaat ttctttcaaa ggttgaatac ttccaatttc attatttgca tgcattacag
     6661 aaattaaaat agtatccgaa cgaatctcgc tttctaattt ctttaaatca attatgccaa
     6721 actcatcaac aggtatataa gaaatttcaa aaccttttgt ttccaaaaac ttacaaacct
     6781 caataacagc aggatgttca atttgggaag taattatatg cttacctcta ttttcgttag
     6841 caaaagcata acctttaatt gcgtaattgt tagcttcgct tccaccactt gtaaaaacta
     6901 tttcagaagc tttacaaccc aaaagattag ctatttttgc tcttgcattt tcaacagctt
     6961 tttttgcatt aactccaaag gaatgagaac ttgaaggatt cccaaaataa tcatttagaa
     7021 atggcttcat aaattccgca acttctttgt ctataggcgt tgttgcatta taatctaaat
     7081 aaattcccat aattgaaatt ttaagcaaaa ttaagaaaaa aaagagatta tctgaaattt
     7141 aataataaat cttacaaaac agtttcatct tgaagattaa atttaggctt tgttttggct
     7201 ttttctaagt ttaaacgtct attttctttg tcttcttttt ttagtttttt aacaaggttt
     7261 acaaccatta aaaaagtaac tgaagctgct aaaaagtcag atattggaaa tgaaaaccaa
     7321 attccatttt cgccccaaaa cttagggaat atcaatacaa aaggaattaa aaataaaacc
     7381 tgtcttgcca aagccaaaaa cattgaagct tttgccttac caagagcttg aaatagccct
     7441 gagccaacaa tttgaaaacc tactacagga aaagcaagta taaaaattct tagcgacttt
     7501 gttgccattg ctattagctc tttatctgtt gtaaaaacac ttactattga gttagcaaaa
     7561 acaaaaaata aaatcaatgt agctatacaa aagcttgttg accaaataat tgctattttc
     7621 atacctcgta aaactctaag tatttttttt gctccgtaat taaatcctgc aataggcata
     7681 aatccttggt ttagtccaaa aagaggcata aacacaaaca ttgttattct atttattatt
     7741 ccgaaagctg ctattgaagc tgcaccgcca tatcgtaaaa gagcattatt aaggactata
     7801 ctcataatag aacctgcaat ttgtcttcca aatgacgaag ctccaattgc taatacttct
     7861 aaagtaattg ccttgtcggg taaagcccat aattttctta tatttaattt taaaactgat
     7921 tttggatttt ttgtaaaata ccacattgct aaacaaaatc ccacaaattg acttatagag
     7981 cttgctaaag ctgcacccat taagccccaa ccaagaccaa aaataaatat agggtcgaaa
     8041 attaaattta aaactcctcc caaaatttgc gaaaacattg ggaatcttgc atttccttcg
     8101 gctcgaattt gatgaactga aacaaagaga atgtttaaga aaaaagagcc aggcaatgac
     8161 caacgaacat attgcacagc ataaggcaaa atttcgtcat ttgctccaaa aagttttaaa
     8221 aagaaaccag cttttaaata agctaatgta atgagtatga agtttattga gataattatc
     8281 gtaagtaaag aatgaagtgt aagttgagcc ttagctttat ttcctgctcc taatgctcgc
     8341 gatattacag aagctcctcc caatcctatt gcttgtccaa aagtagtgat tgacatcatc
     8401 aaaggcaatg aaacagaaat agcagcaatt ccaagagttc cgacagcatg tcctataaaa
     8461 atagtatcgg caacgttgta aatagttacc gacaacattc caatcattgc aggaagcgat
     8521 aagctccaca ataatggttt tattttttct gttcctaatc tctgcgacct atccatcaaa
     8581 aatctttcta cttttctctt aactattttt acttattact aataacttta aattctgttc
     8641 tacgatttaa agcacgtcct tcttctgttg cattatctgc aattggaata gaaaaatcat
     8701 aacctttata tgacaatcta tccttatcta tccctttgga aatcaaataa gtataaacag
     8761 attttgctcg attttcggat aaagttttat tatacgcctt tgatccaaca ttgtcggtat
     8821 gtccgcctat ttcaattttt aagtttggat ttgtattaag ataatccaca acaattttta
     8881 actcagcata ggattctggt aaaagttcgt aagagtcaaa tgcaaagaaa atatttttta
     8941 aaattacagt tactccttct tccaaaggtt gtagagggat atttaatata aaaggttgtt
     9001 ctgggtcttt aatgtttttt aaagaaaaat tttcggaatg aaataaaaat cctgtttttt
     9061 caacattaaa agcataatca cgctctactg gtaaagttac caagtattcg ccagttattt
     9121 tatcagtagt aagccttacc atttcttcgc cagattcgat atcaaaaagt tcgcatttag
     9181 cttctaatct ttgatttgtt tttgcattat aaacatagcc tttaacataa gttgctgcca
     9241 caggtttaat ttcttctgga attaaaaaga aataaatatc caaatctttt cttccaacat
     9301 ttgacgcaaa cattgcaata tcacctcttg catttactat aaggcttctc tcctcaccca
     9361 tagtgttaag tggatagcca aggttcacag gttttgacca atttccatta ttatcaagtt
     9421 ttgaataaaa caaatcaaaa tctcccattc cgggatgacc acgtgaagca aagtacaaag
     9481 ttcttccgtc agggtgaata aatggtgaaa gttcttcgaa ttgagtattt atttgtggac
     9541 caagattttc ggcttcgtca agagcattgc cttctgcatc tattctaact cgccaaatat
     9601 ctgttttacc atagccacca gctcttcctg aagcgaaata taaatatttt ccatcagccg
     9661 aaagcgaagg attgctgtcc caagaagctg tatttataga ttccaaattt tgaggatctg
     9721 tccattttcc acctcttaaa aacattatat ataaatcgca agaacctttg ccttcaggac
     9781 gattacaagc cgtaaaaaca caagtttttc catctgcact aatacattgc gcaccttcgt
     9841 taagttttgt atttatattt tcgggcattg gaatagcttt aataaattgt cctgttttat
     9901 cgtcaaagta agaaacaaat aaatcttcct gcatagatga tggatcacgt gaagtagaat
     9961 taggatttat gggaatttga cgagtaaaaa ccaatactct ctcatctaca gaaagtgagg
    10021 gccaatattc atcaaagtgt gtgtttacac caggtcctat gtttataggt ttaaaatctt
    10081 tagggttttt ataagcacta tctgcaaaat aaataaattc tcttaatctt ttaaaatcat
    10141 aattgcgata cctcattgtg tcaacctcat tacagaaaaa atctatatat tttttagcaa
    10201 ctgaatattt tccgatttta tatgaatttg cagctaattt aaaacttaaa gcagggtcat
    10261 attgagcatc gatttcaaaa acttttgcat aagacaaata agattcgttt gcttttccaa
    10321 gaaaatcata aatctcagct ttcaaaagcc atgcttctaa aaagtttggg tctttttcta
    10381 tagctttttc aatttcggta atagccattc tattttgcct ctgctccatg tatgaaacag
    10441 ctttattaaa actttttatc gctgatttat ttttcgtata aacagaattg ttttgtgctt
    10501 gtacaaaaaa gctgaaagca aaaaatatta ttaaaaaact aaaaattctt ttcattctta
    10561 aaatttttat tcgtttacgg aatacgcaga aatttatgtg tttgcaaaga tacattccat
    10621 tttgggtttt gttttacata atctacaagc attatgccga ttttttcaaa cctactccat
    10681 tctggctgta aaaacaaagc acaagaattg gttacttttt tggcacattc ttcagcccat
    10741 aaaaaatctt tttcttcaaa aatcacaact ttaagttcat cggctatttg aaagtactct
    10801 gcttttggtg gttttgtttg ttttggcgaa agacaaaccc aatcccaaac tccactaaaa
    10861 ttatgagctc cgcaagtttc taacattaaa gtaaaagctt gtgattttgc ttttgcacaa
    10921 aactcatcga aattatgaat taatggttcg ccaccagtaa taacaatagt gtttgcaggt
    10981 gtttgcttta ctctttgtaa aattgcatca atctcaatat attcgtgaat ttctggtttc
    11041 caagtttctt ttgaatcgca ccaatggcaa gctaaatcgc aaccaccagt tcttataaaa
    11101 taagcagcct taccagtgtt aaaaccctcg ccttgaattg aataaaactc ttctaaaagt
    11161 ggaattttac ctgctttcac tatgtctttt aattctttgt tcattatttt ataattttat
    11221 ccaaaacact tcgtgctggt tcaacattaa gcagaatatt catcattttt aatttcacat
    11281 aatcctcatg aaaaaatcta taacctttat tttttccatc aaaagcacct gcaccagaat
    11341 ctttaattaa ataccaatat tctccattaa taatttgata accaactaaa tgcacacaat
    11401 gatcgtctgt tgttgcacca cttatcaaac gcatttggcg agcatcttcg tttatataag
    11461 ccgaaggaat atcaaaatct ggaattattg ccacttcttc tatttgacaa attccaggtt
    11521 ctgaaacatc gccacaaatt gaaattgaat atctattttc aatacttgtg tttatcaaac
    11581 tcataaaatc atttaaagaa atattataat aatctttgca atgccaccag ttatcgtttt
    11641 caacaagctc gcctttctga ttgtaagcaa tttctttggt tgacataaac gaaaaataat
    11701 tattaggttt taatttcagc ttttcgctca taaaagtttg tggtgtataa tttttccctt
    11761 caaaattaaa attatcagga ggcgttccaa tccaatagtc aaggatttta taaatggaat
    11821 ccattatttc ggttttattc catgagtttt gttgtttttg aaaattcaaa taagtttcgt
    11881 attgctcaaa taaagctttg tgattattaa aattttgttg gtcttttaat cccgaatacg
    11941 ctgataaagg cataagacca tgatttttca taattcttaa aacagcgtta gattcactac
    12001 cctcgcctaa aaaagtttca cctttagttt ttataaaatc ttcggctctt tcaacatatt
    12061 cccagtaagc gatataaatt tcggaaagtt ttattttttg gcttgtcatt ctataaactt
    12121 ctgattccaa aaacgaaatt gcagaataag cccagcaagt tccactatta ccttgagaaa
    12181 caggttgaag atgataaact gttgtgtatt ttgttaaatc tgttggaaaa atgcggtctt
    12241 gcatttccat tttaaagact tctctatgct gatattttcg cttagcccaa gcttcgtccc
    12301 caagattatc tcgcaaaatt tgcttgaaat aaaaacccga aggataagtt ttatatgaag
    12361 ccttatctgt gttttgagca aataattgag ccgaaaacat taagaaaatc aaaaataaat
    12421 aagtgtttct catcattgta attttgtaca aagatagaaa taatcagtta aaagttaaaa
    12481 gttgaaagtt aaaagttgtt ttttattttt gggtgttgca acattgcaac attttaaaca
    12541 gcacatatat ataattgtcc gtaaggacaa tataagacta tcaagggttt gtccgtagga
    12601 caaggcctaa ttgttcggca tgggtgttgc aacgctgaaa acaggagatt ttaaactaat
    12661 aaattaagta ttgttaaaaa aatgaaataa aaactaatta taagtattct tcaaatgcct
    12721 ttcataagtt taatgaattt aaaaactgtc ttattcttca aaattaagcc acttctttaa
    12781 agaaaaacct ttaaaattaa tatcttcctt tccaaaataa actttgtatg atactgaagg
    12841 taaaattggg aaaaaactta tttgatataa attcaatttt gcaaaaccat tttcaggacg
    12901 atgaatctct ggactggaat tattattgta ataataaaga taagggtttt gacgattata
    12961 agcattgtaa atagaaaaag tccaaacagt ttttaacttt cgtttttttg atatagtttc
    13021 atatttaagt ccaatatcca aacgatgata gtctttcatt ttactggaat ttcgctctcc
    13081 atatataaaa gcctcataaa tttcattagt atgcggatct acaacatttt gccttcctaa
    13141 aacaggtgtt attggtaatc ctgactgata attccacaca agacttaaac taagtttttt
    13201 atttattttt ctatttataa aaatgctaac atcatgaggt ctattatatt catataaata
    13261 ttcttttcca tcattaatat tatggaactt acgagtagat ttacttaaat gataagaaat
    13321 caaaccagtc cattttccac aattcttttt aaacataaat tcaagaccat aaacttttcc
    13381 cacacctcct gtttctatcg actgtttcca gttttcgagt tctgacaaat agtaaacatt
    13441 tttttctgat gtaattaaat tatataattc tttatcataa acacctatgt taagactaaa
    13501 catattgtca taaaatcctt ttgaccaaga caaagatttt tgtaatgaac ttgaaactgg
    13561 aatatcctta caagaaggaa tccaaatttc attcacataa atatttccag atgatattaa
    13621 caaatgcgat gcttggctaa cctgcatata gttaaagttt atgacaccaa gtttattaaa
    13681 attaaagttt atgtcaaatc ttggttcaat aaaaaactcc tttgaatttg ataaaaagta
    13741 gtttcctacc ctagctccta gttcagtttc aatgaattta aaaaacttaa atttatttga
    13801 caaagaaatt gaagaattat taatttcttc agataaaact tcataataat tttcttttcc
    13861 caaaactctg tagtcatatg gagtataaat aaaatgactc aaatgtagcc ctccaataag
    13921 tgtccaattt ctcataagat taaaagacca aactgaattt aatgaaattt cttgaacttt
    13981 cgattcaaat ttatttttaa atccttgatt tatagaattg ttaaaagatt gtagatcttt
    14041 taccctatat ccagaatatg aaatgtttgt ttttataaaa atttttggac ttataatact
    14101 attccaagaa acagccccca ttaaacttcc ccaaatatta tttgctttta tttttgaatt
    14161 aaataaatct aaatcatctt ttttattcca aatctttaaa taatcatctc cttgaaataa
    14221 attcattgaa attgtgttaa gtgaatttat tttccaaact gacttaaaat ttacatcatg
    14281 aaaaccgtaa gagataaaat aatctcctcc atcagacaag ccactagcgg caatcattaa
    14341 aacatcaaaa agagtttttc tagtagtgaa aatatacgta atcttgtctg taaatttccc
    14401 ctcaagactt aagtttacat cagtcaatcc tatcccgaaa cttcctttga atccattagc
    14461 actacctgaa cgttgagtaa tatcaattat tgatgaaagc tttcctccaa atttagcagg
    14521 gaatcctgat ttataaattg taatattatt tatgatatcg ggattaaaaa cagacataaa
    14581 tccgcctaaa tgatttacat aaattaaagg agtgttatct aaaataaata aattttgtcc
    14641 aggatcgcca cctcttacaa ccaataaact agaagcttca ctttgcgact gaattcctgg
    14701 taatatttga gtagatttca caacatcagg ctgtgcagtt aaacttggaa tttgttcaat
    14761 ttctttaagc tctaattttg ctacatttac agttttatca gattctacac gtattacaac
    14821 ttcgtctaat tgatatgcca atggctttaa ataaatattt tgaatattat tttgtgattg
    14881 atttattttt ataatttgtg tttcatagcc aataaaagac actttaagtt cagttgaatt
    14941 tgttttaatt gaataataag cctgagaatt agtgcttgtt ccgttagaat ttactgaatc
    15001 aacaatattt gctccgataa tagcttcatt tgtttcggca tcatatacat accccgatac
    15061 tgtaacttgt gagttcgcaa gtaaaacaga aagtgtaaat aatataacaa attgaacttt
    15121 aagcataatt agtctattgg agctttaata tcactaagtg tttgagaata tccacaaaaa
    15181 atcccatatc cgttgtttat attagaaaat aaatttgtcg gtactatacc tgcatttgaa
    15241 aaatcaggga aacgacttaa attataattt tcaaaacttt taacatattt ataataatta
    15301 gaatctacag actttattat taattgatat ttataattgt taatattgtt tgaataaaaa
    15361 tgatgtgaaa tttttacact ttttgagata atgtctttct ccaactcaaa attatcaaaa
    15421 aacaaaactg gatattcttc aataacttca gtaacatttt catcatatat cataatatat
    15481 gcttcgaaaa ataaatcttt tgacaaatca tttataattg aaaaatttat ttttggctga
    15541 agtactcctt cgttatcaac ccaagcattt tctataatat gaaactcaga aatatttact
    15601 ctatctggaa taaagcaact cgaagaaaca atactattat ttacatttac ttttaaataa
    15661 aattcgtcat tttcttttgc aatataatca gaaatataat gtcctttttc aacataatta
    15721 aaatttaaca aaacagaatc ctgattatac atttcaatat ttgcattatt tgcatttgat
    15781 aactcataag catctaaacc acttgttagg gaaacatgta aatgaatttg ttcatcttga
    15841 actatgatag aattaattgt tattttgctg ttaaaatcct caaactcatc ttgcacaagt
    15901 tttcggcaag aagaactaaa caaaataatt aaaaaggcaa atattaataa tgttttttgc
    15961 ataaattcag aactttaaaa ttaagcctaa cttaaatcca ttttgaaaat ttgattttac
    16021 tacattttta gaaaaaacaa aactccaagt ataatcaaaa tttaatccta agtttttaaa
    16081 tacaaataat gataaaccag ccccaagatt agtgtaaaaa taatttgaaa agccactatt
    16141 tgttgtctta tacaagaata agcctgtttg atttaacaca taggcatctg ctctaaaaaa
    16201 tctattattt gattcaataa aaaaaggaag gatatgaaat ctattttgaa ttccaacaaa
    16261 atttaaagaa acgaaagatg atgcaaaata tctattgtaa taaacaccag tttctaaaaa
    16321 tttggctaat ccataattta atgaaacgaa aactcctttt tctacttgtt tatattcagg
    16381 cacaagagag taattcattc ttgtaaaaga aacatcacct gttaatcgct tatttatata
    16441 tgaagatttt tgagcattta ctaaaaatgg aaataatatc aagattaaaa atattctgag
    16501 cctcatattg ttttttttgc aatatatgaa atttaattga taacaataat aattattaaa
    16561 aaaaaagccc tactaaaaat tagcagggca aatcaaataa cacatttatt aaacacaatt
    16621 acagtaaatt caacacaaca tttacgttat ttctaattgc ttttgaataa gggcaaactt
    16681 gatgagcttt ttctataagt ttttgtgcag tttctttatc aacaccttga acatcaatat
    16741 caagcacaac agaaattgct aaactaccat cttctacttt tccaatactt acatttgctg
    16801 ttgtagtact tgtaatttta aatctttcca ttctggcaac taaattcaaa gcaccgtcgt
    16861 agcaagccga ataagctgcg ccgaaaaata gttcgggatt aggatagtca tcattttgtc
    16921 cgcccaaact ttttggcatt cttacgtcca tatctataat tccatcagaa ctttttacat
    16981 gaccgtttct tcctccagaa gttgttactt ttgctgtata taaaatttcc atttttaatt
    17041 atttattaag ttaaacatat tttaaattat cagtataaac aaatttttat aaattttgtt
    17101 tttcggaaat ttcagttaat ttactatctt tacaaataag cacaaagtga tacgcagaac
    17161 gtagttgaag tgtcataatg ttgtgttgcc ctacgggcaa agctttcgag ggtctaaata
    17221 ccgcaggcta cgcctacggt taatcacatg ttgtccttcg gacaagctct tgatagtctt
    17281 gttttgtcct cacggtcaac ctacactagt tcaaaccaac acccctgacg ctcgctcgga
    17341 tttagcttcg ctgagctcgt ccttcggact tcggttgtaa tccgagtgta acattaccaa
    17401 cactcagatt ataaatccaa gcgagtggaa aaattatgta agtttttttt caatatacca
    17461 tttcttaaat tattttatgt cttcaatact gtcaaatgac ttttcaaact taccattacg
    17521 aaaactaaaa aacttagcat tgtaaatttc cttaaatgtt gtatcatcaa cttcatctat
    17581 caagtcttta ataaacttcg ataaatattt tgaagttaaa ttcgttttat aatattgtgt
    17641 gtcaatgcct attattagat gtgaaatttt atagttttta ctcatatatt tcgaatcgta
    17701 atattcagca actacgaaaa gttctattcc tttttgcgaa caaatttctt gcaataaatt
    17761 taaagaatag caaaatctac ttttacattt tggcgaccaa atataaatta ttgatttttc
    17821 aaaatttttt agatattttt ttatctcctt tccattagac agaacaattg ttgtagaatc
    17881 gctatatgaa tttaaataat cgttatttgt atcaaatttt aaaaaattaa ttttcccttc
    17941 tttttttgtt ttcttataat aactaaacaa accttgaaaa cttccattta ttgaacatga
    18001 tgttaatgaa cttataaaaa ttaaaaataa aaaaattctg aacttcatat tgtttttttt
    18061 gcaatatatg aaatttaatt gattaaattg ataacaataa atcatttttt aatcaaaaca
    18121 aaaaaaaaca acttttaatt ttcaactttt aacttttaac tgattatttc tatctttgca
    18181 aaaaatttaa aactatggaa tttaactatt atgcttatat tatcttgcct cttctaatat
    18241 ttttagcaag aatttgtgat gttactattg gtacggttag aattatttta gttgctcgtg
    18301 gacacaagat tttagcacca attttaggat tttttgaaat cttaatttgg attatggcta
    18361 ttagtcaaat tatgggcaac ttagataatt gggtttctta cttggcttat gcacttggct
    18421 ttgctacagg taattatatt ggaatgcttg tggaagaaaa aattgcaatg ggaatagttg
    18481 gtttgcgttt agttaccaac aagcctgcaa ctcaactaat agaagctctt aaagaaaatg
    18541 gttacggaca tacacatatg gacgcaaatg gtgctactgg tcctgtaaat gttgtattta
    18601 taactgttaa cagaaaaaat ttaaacgaac taattgattt aatcaatgtt tacaacaaag
    18661 gagcttttta cactgttgaa gatataaaat tagtaaataa aggtatattt atgcaaaaca
    18721 atgcttccac aagaaaactt tttcagcgta aaggaaaata aaatttgaaa tttgaagaat
    18781 taaatattgc tcaaagttta aaagacggca ttgatagctt taattatcaa gaacttacgc
    18841 ctattcaaga aaaaactttg cccataattt tacaaaataa agatttgctt gcttgttcac
    18901 aaacaggttc tggtaaaact gctgcttttt taattccaac tatgaataaa attcttaact
    18961 cagaaaggaa tggaataaaa gctttgattg ttgcacctac tcgcgaaatc tgcattcaaa
    19021 tcgaggaaca aattatggga ttaggctatt ttgcaggtat ttcgtctgta gcagtttatg
    19081 gcggaagtaa tcaagaagat tttactattc aaaaaaatgc tcttgaaaat aaaaccgata
    19141 ttatagttgc tacaccagga cgactactag ctcatctaaa tttaggatat gtaaagctaa
    19201 ataaattagt tactttaatc ttagatgaag ctgacgaaat gcttgatatg ggattttata
    19261 cagatataat gaatatcatt agttatttgc ctaaaaaccg tcaaaatctc ctattctcag
    19321 ctaccatgcc aagctctata tgtaaagttt caccacgttg ttaatgaatt tatccccctg
    19381 cttttgctca t
//
LOCUS       DAOPNX010000044        19010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_3521_length_19010_cov_6.814983, whole genome shotgun sequence.
ACCESSION   DAOPNX010000044 DAOPNX010000000
VERSION     DAOPNX010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19010
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3521_length_19010_cov_6.814983"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1838
                     /locus_tag="PKN53_06305"
     CDS             120..1838
                     /locus_tag="PKN53_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD88596.1"
                     /translation="MPKIRIEYINSSCPTISNLNATVNSDNSVTLSWTNPPQNEFPND
                     CDGDVYFTFFNGYTELGGCGIDSLTSWKTNIFEPDIHSLGVSISYWKNFTENICYTEV
                     IYVEADIPEIPVCPPVSALSVTDNGDNTVSLEWTNPEANELPNGCSHFNFEFYDGSVK
                     IGESNENNLTSWTSGKLSNWEHTLKVRIRYFDNNNNIICFADTHETIWLYDGISCEPV
                     YGLSATVNEDNTVKLEWTNPQQEDLPEGCFYFTFDFYDNNNKIGNSNNSSINNWTTPV
                     LSKGSHNLAVVVRYFNNMNAEICFAEESKTVTIEPINPDLVVHYDDGYNDLSIGMMGK
                     PHTIIAAIRLTNDELADYYNDYKITSIRYFIDTVEAYLTPNIVIKIFGQGTDTTPGQL
                     LTSAPAGWATHRWNTYTLNNPLQITSGDYWVGYEVIPKVAGCPAGASENTVVDGKGDW
                     YYSKKLTEDKWVELKDILSNGYKYNWNIQVTLTSTVGIEEIEISLLAAEIFPNPANDV
                     LNIVSDSKIISYELYDALGRLMINKTNVDNTETIINVSALKHGMYMLRLSTVKGSGMF
                     KVIIDN"
     gene            2003..2314
                     /locus_tag="PKN53_06310"
     CDS             2003..2314
                     /locus_tag="PKN53_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88597.1"
                     /translation="MKIIKLLFFATLMLSLSFGCKMKNTCELNHTGEIAVTNKTGDVL
                     DVYIDNLKVFSLDNLATKTIDKPVGEYKVKCLKYPNEWEQTVIIIECETVSVQFPESE
                     E"
     gene            2314..5508
                     /locus_tag="PKN53_06315"
     CDS             2314..5508
                     /locus_tag="PKN53_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOD88598.1"
                     /translation="MDNLKVYSASAGSGKTHSIANEYINLLFQNSSAWRSILAVTFTN
                     KACDEMKSRIIEELHKIKIATNTEKIKTLAIQNDCTEKEVIDNTQKIFTGILHDYSFF
                     SVLTIDSFFQKILRNFTKETKIQYDYELELDSNKVVSTAVDELLEQSNTDENLRPLIL
                     ELVNEKMQDQKKWDFRSELITYLKNVIKSDYRSFQTQYDVFFKNPENLENLKKELKKI
                     EDDFIKEINSLKKQYEDLLKKYNLTGDDFKGKSRSVINKILKSLTTLQSGVNVDIDNI
                     FDKAFEIEAWLGKDKLNDNVFVAVFNQCYEISAKTQDFLLKNYCTYNSILIISKNLSK
                     TRLINYGLSAIKEYLGRENKFLIDDVPIFLSEIANHNNASFIYEKTGTFYNNFLIDEF
                     QDTSVTQWDSLKPLLEESLAKGFLNIIVGDLKQSIYGWRGGDWKLLAQELKNTFPNYY
                     SEINLEDNWRSGKHIVEFNNSFFKNAIEILSESIKNDVPDDLHEQTSKLLQNTIYENI
                     KQNNKKDFKSEVKIKVFNENNREEILENLVAQIEEIQLNKHPAGSILILVRGNTDGKE
                     IANYILKYSQSENAKPNVTYDVISSDSLFINSNSAVNFIISIFKYLNNNKDEIALSEI
                     YSSYNIDNQEDIDKIFTNKVLESKLQNIQEKIRNKLVYESSEIIINEFELNKKQENIP
                     FLSSFQDLILKFEQRYASEISLFLEFWEEEGKAQNIIVPEKQNAINILTVHKAKGLAA
                     DFVLVPFCNWSMNQYNNTIWVSSNTPPFNALPTWAVNYDNKLRNSVFAEDFHLTKFKN
                     IVEAFNILYVAFTRARKGLYISSIDLTDNTNSKISTFLNKTIASPNFQNELNLTISEN
                     PDASYTEYYFGETPDAEADAKEDVYINTYPVYLANKKIKIKSSFERDKLDTESESSIR
                     KGIVFHKIFENILYSKNISSAVKKLVHSGFIKSSEQDYYIDEVNKIISKPFVSDWFSE
                     KYKVLNESEIIDKTGQVFRPDRIIYNDEKICIIDYKFGEKENSNYVNQVKRYGKLLKD
                     LNYQNIEIYIWYVLLDFLIKVDANSGNTEKIFLK"
     gene            5505..6266
                     /locus_tag="PKN53_06320"
     CDS             5505..6266
                     /locus_tag="PKN53_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD88599.1"
                     /translation="MKRLIRFFVRHIPRPILIRMSGLFSFFVRWFYRGKKVECPICEN
                     KFRKFLPYGSKAGENRLCPVCLSLERHRLIYLYLKNHDNFFSEKYKVLHIAPEQPFLK
                     RFKKLSNLDYTTADLVSPIADLHFDIMKIPLEDNTYDWVICNHVLEHVESDIEAMKEI
                     LRILKPGGKAILQVPINYDYTKTHEDLSITDPKEREKIFGQYDHLRWHGLDYPERLKT
                     AGFQVLEFNIKDFLTPEEIEKYRLDTREILYINSK"
     gene            complement(6404..7807)
                     /gene="rlmD"
                     /locus_tag="PKN53_06325"
     CDS             complement(6404..7807)
                     /gene="rlmD"
                     /locus_tag="PKN53_06325"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOD88600.1"
                     /translation="MAKKKFPIIENLLITSIAAEGVSLGRHNDYVIFVSGVIPGDIVD
                     VQLTRMRKAYAESKLLKIREFSKDRIEPFCKHFGTCGGCKWQMLPYEKQLEYKEKQVY
                     DQLTRLGKLENLNLKPIIPAKNDKYYRNKLEYTFAQRRWIDSNEPFVENTGRNLEGLG
                     FHVRGMFDRIVDIDECFLQVEPSNEIRNKLREFTKRDGYDYYNSRTHEGMMRNLMIRT
                     SSLGEIMVVVIFNRDEKQLIEAVMKFLDEEFPNLTSLQYVVNTKFNDSIYDQDLICYK
                     GRSYILEDLDGLKFRIGPKSFFQTNTEQALVLYRKTVEFADIKADELIYDLYTGTGTI
                     ANFIAKKCKKVVGIDSVPEAIDDAKKNSELNNIDNAVFYAGDMKDLLNSEFIEKNGKP
                     DTIILDPPRAGVHENVINIMREISAKKIVYVSCNPASQARDISMLSDMYNVVEVQALD
                     MFPHTHHVENIVLMEKK"
     gene            7916..8548
                     /locus_tag="PKN53_06330"
     CDS             7916..8548
                     /locus_tag="PKN53_06330"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOD88601.1"
                     /translation="MENSKNNKIILTISSVALLISIAALLLTVFSKNKTVAPQAVNNA
                     VSFSNDSAISGNVAYVNLDTLLLQYKYSIKLNEDLLTEQAKAKANLESRMRAFEKKYN
                     DFAEKMRLGSFLSQQSMESQQNELLQEQANIQQLEQDLTEKLMMKQADLNAELLDSVS
                     NFIKEFNNGRYVLILGSVTGGGILYAEPDMDITNEVVTQLNERYEKAKKQ"
     gene            8556..9803
                     /locus_tag="PKN53_06335"
     CDS             8556..9803
                     /locus_tag="PKN53_06335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD88602.1"
                     /translation="MSFANKYFSRYKTYPKIFENNPCPQTGIIVIIPCYDDEFIFETL
                     KSLEKAQKPICKIEIIVIVNSGEKTPKHIVQKNKEIFLKLNEQAKNEFYKTFNLLVYN
                     IEDTAHKIAGVGFARKLGIDEAVRRFNFLNKPQAIICSLDADSIVSDDYFIEIYNYFE
                     NNPKIKATTFQFKHNFDENIFSLDEIKSCKLYEIYLRYFRLAIKSTGFPYSIHTIGAC
                     FGFRADAYCKVGGMPTRQAGEDFYFLHKLTAVSKIGNINKPIIFPAPRFSDRVPFGTG
                     PTVIQINKTKEYEVYNFKIFEIIKQFFNALPQFYETSNFIELIPSEIIEFYTTEKLIK
                     DINDCKNNSSNIKNFTKRFFSKFDAFFIIQFLNSFDKASKYPAQQVLKMAQQLLNSKN
                     IEISNSNIDEIYEQMLKLDLIEN"
     gene            9813..10304
                     /locus_tag="PKN53_06340"
     CDS             9813..10304
                     /locus_tag="PKN53_06340"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOD88603.1"
                     /translation="MKLFYTDFETPLGKMIAIASETELKLLKFYDDKNIEKELQKLKL
                     EKTEADLEIFKNLKIQIKEYFEGKRKSFDIKHNPFGSDFQIKTWKAIQKISYGSTSVY
                     KELARKISSEKAARAVANACGQNKIVIIIPCHRVIGSNQKIGGYSAGVWRKAFLLNLE
                     NDN"
     gene            complement(10401..11327)
                     /locus_tag="PKN53_06345"
     CDS             complement(10401..11327)
                     /locus_tag="PKN53_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOD88604.1"
                     /translation="MRKIYCIGETVYDIIFHNGKAIESRPGGPVLNTAVSLGRLNQEV
                     YAVGDQADDAVGNIIADFLSTNNVSTKYLNLYQNAKSRILLAFLNDENEPSYSFYKIR
                     FDGELKINYSKPEHDDIVLYGSFTSIKKELRQDFVNFLKQAKEVGALLIYDPNFRPAH
                     IKLRDRVIDYIYENIAYADIVKGSNEDFQNIFQTSTASEIKQIADKCACKNLIYTANK
                     HGVHLFTENFHKIFNVPNIKPVSTVGAGDNFSAGLIYGLMQENVYVENVNNLDYEVWK
                     KIMKTSIDFAIEVCLTYDNYISHDFAEKVKTS"
     gene            11411..12259
                     /locus_tag="PKN53_06350"
     CDS             11411..12259
                     /locus_tag="PKN53_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88605.1"
                     /translation="MKRIFLILALLFSSLVYAQVQENTFPKPNGNYLGINWGLSTGSV
                     RDMLTSPLFYTSVLIYGDFSYFYIDEQNIFELKTATHNGLNAKLISDNFYVGFQSAFD
                     FKLSLLRNVQSISDNDLKHYLGADIDNYMSIRIMPDFMNAALSIDNVSNFAIAYKAEY
                     NYHKKRKEKNFLGLIPYTTNEKFYVFSGRLSVPVISLYARPKFTNPESATVDIEDPLF
                     KGYSLKAKAFSGLSTDLSVSRILKNGNIIKYGYAWMFATNGKNAINRLEFSHHIIYIG
                     LVFKFN"
     gene            12272..13297
                     /locus_tag="PKN53_06355"
     CDS             12272..13297
                     /locus_tag="PKN53_06355"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOD88606.1"
                     /translation="MKIKIFSIVLIFIIALSACEKLAFDKKIDGSNISTFNYLWEQVG
                     QKYAFLEYKEVDWDSIYFVYRPMVNNEISEDSLFNVMAKMLNELKDGHVNLISPFNVS
                     RYDITMLGPVNIDTRLIKENYLFDDYYSTGGFVHTAIRNGDIAYIRYESFADSQITDY
                     QLDYLMDKYENTKGMIIDIRQNGGGYVTNVPMLLSRFTHEDTKIYETQIKTSASEDGF
                     SVLEDVYVKSKDKKKYKQKVAVLIDRGSYSASSFFAVSTYAYDNIFLVGDYTGGGLGL
                     PNGGQLPNGWTYRFSITRTIAVDGKNYENGVKPDYYVILNPNAYNNGIDNVIEFAADK
                     LLENNTK"
     gene            13303..14130
                     /gene="pyrF"
                     /locus_tag="PKN53_06360"
     CDS             13303..14130
                     /gene="pyrF"
                     /locus_tag="PKN53_06360"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOD88607.1"
                     /translation="MNYTELFEQIKAKQSFLCIGLDSDFEKLPKHLLNSEFPIFEFNK
                     QIIDETAKYAVAYKPNLAFYESLGLAGWQSLEMTIEYISDNFPEIFIIADAKRGDIGN
                     TSKLYAKAFFDKMDFDAVTVAPYMGEDSVKPFLTYENKWVILLALTSNKGADDFQYFT
                     ENGLRLFEKVLEKSQEWGNSENMMYVVGATRAEMLTDVRKIAPDHFLLVPGIGAQGGS
                     LHEVAKFGMNKNCGLLVNSSRAIIFADASENFAKTAGIKAKEVQEEMQNLLKSKGII"
     gene            complement(14166..14236)
                     /locus_tag="PKN53_06365"
     tRNA            complement(14166..14236)
                     /locus_tag="PKN53_06365"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14202..14204),aa:Cys,seq:gca)
     gene            complement(14303..15244)
                     /locus_tag="PKN53_06370"
     CDS             complement(14303..15244)
                     /locus_tag="PKN53_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HOD88608.1"
                     /translation="MDYLYIIIGIILLILSGNYLVKSSSNLAKYFKIPPIIIGVTIVS
                     FGTSAPELFVSASASLNGLSDVAIGNAIGSNIANLGLVLGLSALIVAVPIKKSLIKLD
                     YSVLMFSAILFGVLALDYKITRLDGAILILAMLIYIFIIIRLLKKNKIETAETEEESQ
                     QKIYHPFVSLLIIIASCGGLVFGSDFLVDGAVSLATKMGVSERIISLTVVAIGTSLPE
                     LTTSVIAAIKRNFDISIGNVVGSNIFNTLFIVGTSACINPLTVNPSTLKIDYPAMLIF
                     YVLLGVFFLPVKRLRISRFEGLILIIAYVIYSYIIFS"
     gene            complement(15254..16639)
                     /locus_tag="PKN53_06375"
     CDS             complement(15254..16639)
                     /locus_tag="PKN53_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD88609.1"
                     /translation="MSKIGLIIKREYTTRVKKKSFIVMTILGPILFASLMILPAVLMQ
                     LEDDEFKRIAVIDETGVFVNSLTNTEYIEFYDFSGRYFDQKTFTYDVTKATDDLKDSD
                     YYALLYIPKNAVNNIKGSVIFYSYTQPNVGIKMHIAHNIENKLKNIKFQVTADDLQIT
                     QEQIDEILSVANSEIQVVTNIIDEKGEFKETHSEVAMIVGYVSGFLIYMFVLMYGTQV
                     MRGVIEEKTNRIVEVIVSSVKPFQLMMGKIVGVALVGITQFALWVLLTIGLVSAGQAI
                     VLKDYDASKLNPTVMEMGSELDATSVVPDEKAMEFEEIFQAVYSVNYFAVIACFLFYF
                     IGGYLLYGAMFAIVGSAVDNEADTQQFMLPITLPMVLGLFVMLTAMQNPNGSLAYWFS
                     IIPFTSPIVMMARIPYGVPYIDLFISMALLVITFVFTTWFAGKIYRVGILMYGKKVNY
                     KELWKWFRYKN"
     gene            complement(16649..17581)
                     /locus_tag="PKN53_06380"
     CDS             complement(16649..17581)
                     /locus_tag="PKN53_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD88610.1"
                     /translation="MNALVAENVEKKFANHLALDDVSITVPEGSVYGLLGPNGAGKTT
                     LIRIINQITAPDKGQVFIYGEKMQSKHVENIGYLPEERGLYKKMKVGEQALYLCQLKG
                     LSKAETLKRLKYWFEKFEIQSWWDKKVEELSKGMQQKVQFIITVLHKPKLLIFDEPFS
                     GFDPINTKMLKDEILNLRNEGATIIFSTHNMASVEEICDHIALINKSRKILEGPINEI
                     RNNYASDIYEITFEGYFNKLESTLTSDYKILDVVENSQKSTMKVEVLNKNIKSNDLIT
                     IMTQYGNISSFQKHLPSMNDIFIKVVEETNTLIK"
     gene            complement(18074..18931)
                     /locus_tag="PKN53_06385"
     CDS             complement(18074..18931)
                     /locus_tag="PKN53_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88611.1"
                     /translation="MKKNIIYLLILSLGIFAFSCDPDDPTPEPIQQAPSIDQTINAIS
                     EYALVNKIFSDSFSEADKAAKNGDKQLEAQKYGQAGDYPVVTITPLDTTTWPKNIKVD
                     YGTSNYVCNDGVKRRGIINIQTTGFYREAGTEITVTYDGFYHNDHKVEGTMKVVNAGR
                     NDNNNLVYNVTIAEGKITTPQDKIIYFNENTSREWAAGENTVFDPCDDNYLIGGSQDG
                     MSSDSIEYTLTVQQKLDILACCKYVRAGVLKISITGILPFTVNFDGSTCDEANIVATI
                     NGAECPITM"
BASE COUNT         6856 a         2953 c         2815 g         6386 t
ORIGIN      
        1 ctatgacact ttttccttat cttaatgttt tataatagaa cgcaacttgg tataatattt
       61 caagacatat ttatggtagt gcatacgagt ataatcacaa tggtacaact tctaactttt
      121 tgccaaaaat acgtattgaa tatataaata gttcttgtcc aactattagc aaccttaatg
      181 caacagtaaa ttccgataat agcgtaactc taagctggac taatcctccg caaaacgaat
      241 ttcccaacga ttgtgatgga gatgtatatt ttactttttt caacggatac acggaacttg
      301 gtggttgcgg aattgacagc ttaacttcgt ggaaaacaaa tatatttgaa cctgatatac
      361 actctctagg cgtgtcaata tcgtattgga aaaattttac tgaaaatata tgttatactg
      421 aagtaatata cgtggaggct gacattcctg aaattccagt ttgcccacct gtttcagcac
      481 ttagtgtaac agacaacggc gacaatacag ttagtttaga atggactaat ccagaagcaa
      541 atgaattgcc aaacggctgt tctcacttta attttgagtt ttatgatgga agcgttaaaa
      601 taggtgaaag taatgaaaac aatttaacat catggacaag tggtaaactt tcaaactggg
      661 aacatacatt aaaagttaga atacgctatt tcgacaacaa taataatatt atttgctttg
      721 ccgatactca tgaaactata tggctgtacg atggtattag ttgcgaacct gtttatggac
      781 ttagtgcaac agtaaacgaa gataatacag taaaattaga atggacaaat ccacaacaag
      841 aagacttgcc cgaagggtgt ttttatttta cctttgattt ttatgataat aataacaaaa
      901 ttggcaatag taataactct tctataaata attggacaac gccagtactt tcaaaaggta
      961 gtcataactt agctgttgtg gtacgttatt ttaacaatat gaatgcagaa atttgttttg
     1021 ccgaagaatc caaaacagta acaatagagc caataaaccc tgaccttgta gtacattatg
     1081 atgatggcta caatgaccta tctataggaa tgatgggcaa accacacaca attattgcag
     1141 caataagact aaccaacgat gaattagctg actattacaa tgactacaaa attacaagta
     1201 ttagatattt tattgatact gttgaagctt acttaacacc aaatattgtt ataaaaatct
     1261 ttggacaagg aactgatact acaccaggac aattattaac aagtgcaccc gccggttggg
     1321 caacacatcg ctggaatact tatacactta acaacccgct acaaataaca tctggcgact
     1381 actgggtagg ctacgaagtt ataccgaaag tagctggctg tcctgcagga gcatctgaaa
     1441 atactgttgt tgatggaaaa ggtgattggt attacagcaa aaagctaaca gaagataaat
     1501 gggtggaact aaaagatatt ctttctaatg gttacaaata caattggaat atccaagtta
     1561 cactaacctc tactgtggga attgaagaaa tagaaatttc gctcttagca gcagaaatat
     1621 ttccaaaccc tgcaaatgat gttctaaaca ttgttagcga tagcaaaatt ataagctacg
     1681 aactatatga tgctcttggc agattgatga taaataaaac aaatgtggac aatactgaaa
     1741 ctattataaa tgtttctgcc ctcaaacacg gaatgtatat gcttagactt agtactgtga
     1801 aaggaagcgg aatgtttaaa gtaataattg ataattagaa attagtactt agttatatag
     1861 atttttaagg gcggacttgt gttcgccctt ttttgttttc aaagttcttt caacttgaca
     1921 aactttataa acttttaact gaagtatgtt ttgaaaaatt gttttaaatc tttaattttg
     1981 aacaaatttt aattttaaac aaatgaaaat tattaaatta ttattttttg caactttaat
     2041 gctaagtttg tcttttggtt gcaaaatgaa aaacacttgc gaactaaacc atactggtga
     2101 aattgcagta acaaacaaaa ctggcgatgt tcttgatgtt tatattgata atcttaaagt
     2161 tttttcttta gacaatcttg caacaaaaac catagataag cctgttggtg agtataaagt
     2221 gaaatgttta aaatacccca acgagtggga acaaactgta attattatag aatgtgaaac
     2281 agtaagcgtt cagtttcctg aatctgagga ataatggaca atcttaaagt ttattcggca
     2341 tctgctggtt cgggcaaaac tcatagtatt gctaacgaat acataaactt attgtttcaa
     2401 aatagttcgg cttggcgaag tattttagct gttactttta caaataaagc ctgcgacgaa
     2461 atgaagtcga ggattattga agaactacat aaaattaaaa ttgctaccaa caccgaaaaa
     2521 attaaaactt tagcaataca aaatgattgt acagaaaaag aagttattga taatacacaa
     2581 aaaattttta ctgggatttt acatgattat tctttctttt ctgttttaac aatagacagc
     2641 ttttttcaaa aaatattacg caatttcact aaagaaacaa aaatccaata cgactatgaa
     2701 ctagaacttg actctaacaa agttgtaagc actgcagtag atgaacttct tgagcaatca
     2761 aatacagacg aaaaccttag accacttata cttgaattgg ttaacgaaaa aatgcaagac
     2821 caaaaaaagt gggatttccg ctccgaacta atcacttacc taaaaaatgt aataaaatcc
     2881 gactatagaa gctttcaaac acaatatgac gtttttttta aaaatcctga aaatctcgaa
     2941 aatttaaaaa aggaacttaa aaaaattgaa gacgatttta taaaagaaat taattcttta
     3001 aaaaaacaat acgaagactt attaaaaaaa tacaatctca caggcgatga ttttaaaggg
     3061 aaatcaagat ctgtaattaa taaaatttta aaatccttaa cgaccttaca atcaggcgtc
     3121 aacgttgata ttgacaatat ttttgacaaa gcttttgaaa ttgaagcctg gcttggaaaa
     3181 gataaattaa acgataatgt gtttgtagcg gtttttaatc aatgctacga aatatctgct
     3241 aaaacacaag attttttact aaaaaattat tgcacttaca atagcatttt aataatttca
     3301 aaaaatctat ctaaaactag attaatcaat tatggtttaa gtgcaataaa agaatattta
     3361 ggaagagaaa ataaattttt aatagatgat gttcctattt tcctttcaga aattgctaac
     3421 cataataatg catcttttat ttatgaaaaa accggtactt tttacaataa ttttctaatt
     3481 gacgaatttc aagacactag tgtaacacag tgggactctc taaaacctct tttggaagaa
     3541 agtttggcta aaggttttct aaatataatt gttggagatt taaaacagtc tatttatggt
     3601 tggcgtggag gcgattggaa attattagct caagaactaa aaaatacttt tccaaattat
     3661 tattctgaga taaatctaga agacaactgg cgaagtggaa agcacattgt ggaatttaac
     3721 aattcgtttt tcaaaaacgc tatagaaatt ttatcagaaa gcataaaaaa cgatgtacca
     3781 gacgatttgc acgagcaaac ttctaaatta ttacaaaata ctatttacga aaacattaag
     3841 caaaacaaca aaaaagattt caaatcagaa gttaaaataa aggtttttaa tgaaaataat
     3901 agagaagaaa ttttagaaaa tttagtagct caaatcgaag aaattcaact caacaagcac
     3961 ccagctggct cgattttaat tttggtgaga ggcaataccg atggtaaaga aattgcaaac
     4021 tatattttaa aatattctca aagcgaaaac gctaaaccaa atgtaactta tgatgtaatt
     4081 tcttctgatt ctttgttcat taattcaaat tcagctgtga attttattat ttcaattttt
     4141 aaatacttga ataataataa agacgaaatt gctctttcag aaatctattc ttcgtacaat
     4201 attgataatc aagaagatat tgataaaata tttacaaata aagttttaga atccaaactt
     4261 caaaatattc aagaaaaaat tagaaataaa ctagtttacg aaagttccga aattattata
     4321 aatgaatttg aattaaataa aaagcaggaa aacattcctt ttcttagttc ttttcaagat
     4381 ttaatattga aatttgaaca aagatatgct tctgagatta gcttgtttct tgaattttgg
     4441 gaagaagaag gtaaagctca aaatataata gttccagaaa aacaaaatgc aataaacata
     4501 cttactgttc acaaagccaa aggtttagca gcagattttg tactagttcc attttgtaac
     4561 tggtcgatga atcaatataa taacactatt tgggttagtt caaacactcc cccatttaac
     4621 gctttaccaa cttgggcagt aaactacgac aataaacttc gcaattctgt ttttgccgaa
     4681 gattttcatc ttacaaaatt caaaaatatt gttgaagctt ttaatatttt gtatgttgca
     4741 tttaccagag ctagaaaagg gctgtacatt tcaagcatag accttacaga taatacaaat
     4801 tctaaaattt caactttttt aaataaaact attgccagcc caaattttca aaacgaattg
     4861 aatttaacaa tttcagaaaa tcctgatgct tcttacacag aatattattt tggcgaaaca
     4921 cctgatgctg aagcagatgc aaaagaagat gtttatataa atacttatcc tgtttattta
     4981 gcaaataaaa aaataaagat aaaatcttct ttcgaaagag ataaattaga tacagaaagt
     5041 gagtcttcaa ttcgtaaagg aatagttttt cacaaaattt ttgaaaacat tttatactca
     5101 aaaaatattt cttcagcagt gaaaaaactt gtacattcgg gctttatcaa aagttctgaa
     5161 caagattatt atatagatga agtaaataaa atcatatcaa aaccttttgt aagcgattgg
     5221 ttttcagaaa aatacaaagt tcttaacgaa agtgaaataa ttgataaaac aggtcaagtt
     5281 tttagacccg acagaattat ttataatgat gaaaaaattt gcataataga ttataaattt
     5341 ggcgaaaaag aaaattctaa ttatgtaaat caagtgaaaa gatatggtaa attactgaaa
     5401 gacttgaatt accaaaacat agaaatttat atttggtatg tattattaga ttttttaatt
     5461 aaagttgatg caaattcagg taatactgaa aaaatatttt taaaatgaaa agattaatac
     5521 gcttttttgt acgacatata cctcgaccaa tattaataag aatgtcgggt ttattttcct
     5581 tttttgttcg ctggttttac cgtggtaaaa aagttgaatg tccaatttgc gagaataaat
     5641 ttagaaagtt tttgccttat ggtagcaaag ctggcgaaaa tcgcttatgt ccagtttgct
     5701 tatcattaga gagacacaga ttaatatatt tatatttaaa aaatcacgat aatttctttt
     5761 cagaaaaata caaagtttta catattgcac ccgaacagcc atttttaaaa agatttaaaa
     5821 aactttcaaa tttagattat accactgccg acttagtttc tccaattgca gatttacatt
     5881 ttgacattat gaaaattcca ctcgaagaca atacttacga ctgggtaatt tgcaatcatg
     5941 tgttagagca tgttgaaagc gacatagaag ctatgaaaga aattttaaga attttaaaac
     6001 ctggtggtaa agctattttg caagtaccta taaattacga ttacacaaaa actcacgaag
     6061 atttaagcat taccgaccca aaagaaagag aaaaaatatt tggtcaatac gaccacctta
     6121 gatggcacgg cttagactat cccgaaagac taaaaacagc tggttttcaa gtacttgaat
     6181 ttaatattaa ggattttctt actcccgaag aaattgaaaa atatagatta gacactaggg
     6241 aaatattata tattaattct aagtaaactt tgcaaatatt tttgatttta tgctaccata
     6301 agtcagttaa aagttaaaag tttttaaagt tttccaaaca ctcaacactc tccaactcaa
     6361 aactcaacac taaaaactaa aaactcttta aactttaact gagctatttc ttttccatca
     6421 aaacaatatt ttcaacatga tgagtatgtg ggaacatatc tagtgcttgg acttccacaa
     6481 cattatacat atcgctaagc attgaaatat ctcttgcttg actagcggga ttgcaactaa
     6541 cataaactat ttttttagca gaaatttcac gcataatatt tattacattt tcatgcactc
     6601 ctgctcgtgg tgggtctaaa attatagtgt caggtttgcc atttttttca ataaattcag
     6661 aatttaataa atctttcata tcgcctgcat agaatacagc attgtcaata ttgtttaatt
     6721 cagaattttt cttagcatca tcaatagctt ctggcacaga atcaatacca acaacttttt
     6781 tacatttttt cgcaataaaa ttagcaatag tacctgttcc agtatataaa tcataaatca
     6841 attcatcggc tttaatatca gcaaattcaa cagtttttct atataatacc aaagcttgtt
     6901 ccgtattggt ttgaaaaaaa gattttggtc ctattctaaa ttttaagccg tccaaatctt
     6961 ctaaaatata gcttctgcct ttataacaaa tcaagtcttg gtcgtaaatt gaatcattaa
     7021 actttgtatt tacaacatat tgcaaagaag ttaaatttgg aaattcctca tctaaaaatt
     7081 tcataaccgc ttcaataagt tgcttctcat cacgattaaa aatcactact accattattt
     7141 ctccaagtga actagttcta atcataagat tacgcatcat tccttcatgt gttcggctat
     7201 tgtagtagtc gtaaccatcg cgttttgtga actctcgcaa cttatttcta atttcattag
     7261 aaggctctac ctgtaaaaaa cattcatcaa tatctacaat tctgtcaaac attcctctca
     7321 catgaaagcc caaaccttcc aaatttcgtc ctgtattttc cacaaaaggc tcattagaat
     7381 caatccaacg cctttgagca aaagtatatt caagtttgtt tctgtaatat ttatcgtttt
     7441 ttgcaggtat aattggtttt aaattcaaat tttctagctt acccaaacga gtaagttggt
     7501 cataaacttg tttttctttg tattccaact gtttttcgta aggcaacatt tgccatttgc
     7561 aaccaccaca tgtaccaaaa tgtttacaaa atggttctat cctgtctttt gaaaactcgc
     7621 ggattttcaa cagcttagat tcagcataag ccttacgcat acgagttaac tgaacatcaa
     7681 caatatcgcc aggaataacg cctgatacaa aaatcacata gtcattatgt ctgccaagcg
     7741 atacgccctc agcagcaatg ctagtaatta gaagattttc aataatagga aatttctttt
     7801 ttgccacgat aaatattttt tgcaaattta aaaagtaaaa ttattttatc tcaatattaa
     7861 aacaattcac aaaaaaaaac taaatttgca taattaatac aattaaaaat tcattatgga
     7921 aaattcaaaa aacaataaaa ttattttaac cattagcagt gttgctttat taattagtat
     7981 agctgcctta cttctcacag tatttagcaa aaataaaact gttgctccac aagcagttaa
     8041 caatgctgta agtttttcaa atgattctgc gataagtgga aatgttgctt acgtaaattt
     8101 agatactttg cttttgcaat ataaatactc tataaagcta aacgaagatt tattaactga
     8161 gcaagctaaa gctaaggcta atttagaatc aagaatgaga gcttttgaga agaaatacaa
     8221 cgattttgct gaaaaaatgc gtttaggttc tttcttgtct caacaaagca tggaatctca
     8281 acaaaacgaa ttgttacaag aacaagcaaa tatacagcaa ttagagcaag atttaactga
     8341 aaaacttatg atgaaacaag ctgacttgaa tgctgaattg cttgattcag tttctaattt
     8401 cattaaagaa tttaataatg gcagatatgt tttaatactt ggcagcgtta ccggtggcgg
     8461 tattctttat gctgagccag atatggacat tacaaacgaa gtagtaactc agcttaacga
     8521 aagatacgaa aaagcaaaaa aacaataatc taaaaatgag ttttgctaac aaatatttta
     8581 gtcgatacaa aacttatcca aaaatatttg aaaataatcc ctgtccacaa acagggatta
     8641 ttgttattat tccatgctat gatgacgagt ttatttttga aacactaaag agtttagaaa
     8701 aagctcaaaa accaatttgc aaaattgaaa taattgtaat tgttaattca ggagaaaaaa
     8761 ctccaaagca tattgttcaa aaaaataaag agatttttct aaaactaaac gagcaagcaa
     8821 aaaatgaatt ttacaaaact tttaatttat tagtttacaa tatagaagac acagctcata
     8881 aaattgcagg cgttggtttt gctcgaaaac tcggaataga tgaagctgtt agacgattta
     8941 attttttaaa caaaccccaa gcaattattt gctctcttga tgcagatagc attgtatctg
     9001 acgattattt tatcgaaatt tataattact ttgaaaataa tccaaaaatt aaagctacca
     9061 catttcagtt caaacacaac tttgacgaga atatattttc gcttgatgaa attaaatctt
     9121 gcaaacttta tgaaatatat ttaagatatt ttcgcttagc cataaaaagt acaggcttcc
     9181 cctactccat tcatacaata ggtgcttgct ttggctttag agccgatgct tattgcaaag
     9241 taggcggtat gccaacacga caagctggcg aagattttta ttttctgcac aagcttacag
     9301 ctgtttctaa aattggaaat ataaataaac ctattatttt tcctgctcca cgcttttcgg
     9361 acagagtgcc tttcggtaca ggaccaacag tgatacaaat aaacaaaacc aaagaatacg
     9421 aagtttataa ttttaaaata tttgaaatta tcaaacaatt cttcaatgct cttccacaat
     9481 tttatgaaac gtcaaacttt attgagctta ttccttcgga aattattgaa ttttatacaa
     9541 cagaaaaact tattaaagac ataaatgatt gtaaaaataa ttcgtctaat attaaaaatt
     9601 ttacaaaaag atttttctca aagtttgatg cgttttttat aattcagttt cttaatagtt
     9661 ttgacaaagc gagcaaatat cctgcacaac aagttttaaa aatggcacaa caactgttaa
     9721 attcaaagaa tattgaaata tcaaactcta atattgatga aatttatgag caaatgttaa
     9781 aacttgattt aattgaaaat taaaacccta aaatgaaatt attctataca gattttgaaa
     9841 ctccacttgg taaaatgatt gcaattgctt ctgaaacaga attaaaattg ctcaaatttt
     9901 atgatgataa aaacatagaa aaagaattac aaaaactcaa attagaaaaa actgaagctg
     9961 atttagaaat ttttaaaaat ttaaaaattc agataaaaga atattttgaa gggaaacgca
    10021 aaagttttga tataaaacat aaccctttcg gtagtgattt tcaaattaaa acttggaaag
    10081 ctattcaaaa gataagttac ggctcaacaa gtgtttacaa agaactggca agaaaaataa
    10141 gttcagaaaa agctgcaaga gccgttgcta atgcatgtgg acaaaataaa atcgtaataa
    10201 taattccatg tcatagagtc ataggttcaa atcaaaaaat tggtggctat agcgctggag
    10261 tttggagaaa agcgttctta ttgaatttgg aaaacgacaa ttaaaagtta aaagcgatgc
    10321 actgagcgta gttgaagtgt taaaagttga aactggttta actttacgaa ctttaaaaac
    10381 tttatgaact ttacaaactt ttaactggtt ttaactttct cagcaaaatc atgactaata
    10441 taattgtcgt atgttaagca aacttctatt gcaaaatcga tagatgtttt cataattttt
    10501 ttccaaactt cgtaatctag attattaaca ttttctacat aaacattttc ttgcattaat
    10561 ccgtaaatta atcctgctga aaaattatct ccagcaccta ctgtagaaac aggtttaata
    10621 ttggggacat taaatatttt atgaaaattt tctgtaaata aatgcactcc atgtttattt
    10681 gctgtataaa ttaaattttt gcaagcacat ttatctgcaa tttgttttat ttccgaagcc
    10741 gtagaagttt gaaaaatgtt ctgaaaatct tcattagagc cttttactat atcggcataa
    10801 gcaatatttt cataaatata atctattact ctatcgcgaa gttttatatg tgcaggtcta
    10861 aaattagggt cgtaaattaa taaagctcca acttcttttg cttgttttaa aaaattaaca
    10921 aaatcttgtc ttaattcttt ttttattgat gtaaatgagc cgtaaagaac aatgtcgtca
    10981 tgttcaggtt tggaataatt aattttcagt tctccatcaa atctaatttt atagaaacta
    11041 taagaaggct cgttttcatc gtttaagaag gctagtaaaa ttcgagactt agcattttgg
    11101 tataagttca aatattttgt agaaacatta tttgttgata aaaaatctgc aattatattt
    11161 ccaacagcat catcggcttg gtcgccaacg gcatagactt cttggtttaa ccgccctaaa
    11221 gaaactgctg tatttaagac tggtccacct gggcgcgatt ctattgcctt tccattatga
    11281 aaaataatat cataaacagt ttcgcctata caataaattt ttctcataat taaaggtttt
    11341 agacatgaca aaaataagaa aatcttagaa attaatactt gcattttgta tatttgcaaa
    11401 aacatttttt atgaaaagga tttttttaat tttagcccta ttattttcaa gtcttgttta
    11461 tgctcaagtt caagaaaaca catttccaaa gcctaatggg aattatcttg gtattaattg
    11521 gggtttatcg actggctctg tgagagatat gcttacctct cccctatttt atacctctgt
    11581 tttgatttat ggagattttt cttattttta tattgatgag caaaacattt ttgaattaaa
    11641 aactgctact cacaatggat taaatgcaaa acttatttct gataattttt atgttggttt
    11701 tcaaagtgca tttgatttta aattgtcatt gttaagaaat gtgcaatcaa tttcggacaa
    11761 tgatttaaaa cattatttag gcgcagacat tgacaattac atgagtatta ggataatgcc
    11821 agattttatg aatgctgctc ttagcattga taatgttagt aattttgcaa tagcttataa
    11881 agccgaatat aattatcaca aaaaacgaaa agaaaaaaat tttttaggct taataccata
    11941 tactactaat gaaaaatttt atgtgttttc gggaaggctt tcagttcctg taatttcttt
    12001 atatgctcgt cctaaattca caaaccctga aagtgctaca gttgatattg aagaccctct
    12061 ttttaaaggt tattctttaa aagcgaaagc attttctgga ctaagtacag acttgtctgt
    12121 ttcgcgaatt ttaaagaatg gaaatataat taaatatggt tacgcgtgga tgtttgcaac
    12181 aaacggtaag aatgccatta accgtttaga gttttctcac catattattt atatcggctt
    12241 agtttttaaa tttaattaaa atcataaaat tatgaaaatc aaaatattca gtatagtttt
    12301 aatttttatt atagctctat cggcttgtga aaaacttgct tttgataaaa aaatagatgg
    12361 ttctaatatt tcaacattca actatttgtg ggaacaagta ggtcaaaaat atgcatttct
    12421 tgaatataaa gaagtagatt gggatagcat ttatttcgtt tatcgcccta tggttaataa
    12481 cgaaattagc gaagatagtt tgtttaatgt tatggcaaag atgctaaacg aactaaaaga
    12541 tggacacgta aaccttatat cgccttttaa tgtatcaaga tacgatatta caatgttagg
    12601 acctgtaaat atcgatactc gcttaattaa agaaaattat ttgttcgacg attattattc
    12661 tactgggggt tttgttcata ctgctattag aaatggcgat attgcttata ttagatatga
    12721 gtcttttgca gattcacaaa ttacagatta tcaattggat tatttaatgg ataaatatga
    12781 aaatacaaaa ggaatgataa tagacataag acaaaatggt ggcggttatg ttactaatgt
    12841 gcctatgctt ttatcgcgtt ttactcacga agacacaaaa atttatgaaa ctcaaataaa
    12901 aacaagtgca agcgaagatg gtttttctgt tttggaagat gtttatgtaa aatcaaaaga
    12961 taaaaagaaa tataaacaaa aagtagctgt cttaatagat agaggttctt attctgcctc
    13021 ttcatttttt gcagtttcta cttatgctta tgataatatt tttcttgttg gcgattatac
    13081 tggcggtggc ttaggattac caaatggtgg tcaactgcca aatggctgga catatcgttt
    13141 ttcgattaca agaacaatag cggttgatgg taaaaattac gaaaacggcg taaagccaga
    13201 ttattatgta attttaaatc caaatgcata taataatggc attgataatg ttattgaatt
    13261 tgcagcagac aaacttttag aaaacaatac aaaataataa aaatgaacta tacagaatta
    13321 tttgaacaaa ttaaagcaaa acaaagtttt ttatgtattg ggcttgattc tgattttgaa
    13381 aaattaccta aacatctttt aaattctgag tttcctattt ttgaatttaa taagcagatt
    13441 attgatgaaa ctgcaaaata tgctgttgct tacaaaccga acttggcttt ttacgaaagc
    13501 ttaggtttag ctggttggca aagtttggaa atgacaattg aatatattag tgataatttt
    13561 cctgagattt ttattattgc agatgcaaaa cgaggcgata ttggaaatac ttcaaaactt
    13621 tatgctaaag cttttttcga caaaatggat tttgatgctg taactgttgc accatacatg
    13681 ggcgaagatt ctgtaaagcc ttttttaact tacgaaaata aatgggtgat tttattagca
    13741 ctaactagca acaaaggtgc tgatgatttt caatatttta cagaaaacgg tttacgttta
    13801 tttgaaaaag ttttagaaaa atcccaagaa tggggaaatt ctgaaaatat gatgtatgtt
    13861 gttggagcaa cacgtgccga aatgctaact gatgttagaa aaatagcccc agaccatttt
    13921 ttattagttc ctggaattgg cgctcaaggc ggtagcctac acgaagttgc aaaatttggt
    13981 atgaataaaa actgtggact tttagtaaat tcttcgcgag caattatttt tgctgacgca
    14041 agcgaaaatt ttgcaaaaac agctggtatt aaagccaaag aagttcaaga agaaatgcaa
    14101 aatttattaa aatcaaaagg gataatttaa aaatttactg gataattgct tatcgtctgt
    14161 gagagaggta cctggcggag tcgaaccgcc gtagccggtt ttgcagaccg gtgcctagcc
    14221 actcggccaa ggtaccattt tgttttggga ttgcaaatat agaaattaat ttttattatg
    14281 caaaattttt taagctaaac tcttaagaaa aaataatata agagtaaata acataagcaa
    14341 taattaaaat caaaccttca aatcggctaa ttctcaaacg tttaactggt aggaaaaaaa
    14401 ctccgagcaa aacatagaaa atcaacatag caggataatc tatttttaaa gttgatggat
    14461 ttactgttaa tgggtttata caagcactag ttccaacaat aaacaatgta ttaaatatgt
    14521 tagagccaac aacatttcct atagatatat caaaatttcg tttaattgca gctattacag
    14581 aagttgttaa ttctggcaaa ctagtcccta ttgccacaac tgtaagcgaa ataattcttt
    14641 cgctaactcc catctttgtt gctaagctta cagctccatc tactaggaag tctgagccaa
    14701 aaacaagacc tccacaactt gcaataataa taagtaaaga aacaaaagga tgataaattt
    14761 tttgctgact ttcttcttct gtttcagcag tttcaatttt atttttcttc agcaatctta
    14821 ttataataaa aatgtaaatc agcattgcta agattaaaat tgctccatca agtcttgtta
    14881 ttttatagtc tagagctaaa acaccaaata aaattgcaga aaacattagt acagaataat
    14941 ccaacttaat caagctcttt ttgattggta cagctacaat taacgcactt aatcccaaaa
    15001 ccaatcctag atttgcaata ttacttccaa tagcatttcc tattgccaca tcagaaagac
    15061 catttaacga agcacttgca ctgacgaata gttctggtgc tgaagtccca aacgaaacaa
    15121 ttgtaactcc aataattatt ggtggaattt taaagtattt agctaaatta gaactagatt
    15181 taactaaata atttcccgaa agaattagca gaattatgcc aattataata tataaatagt
    15241 ccataagtct tgtttagttt ttatatctaa accatttcca caattctttg tagttcactt
    15301 ttttgccgta cattaaaatt ccaactctgt aaattttgcc agcaaaccaa gttgtgaaaa
    15361 caaatgtaat cactaaaagt gccatagaaa taaacaagtc gatatatggt actccataag
    15421 gtattcttgc catcattaca attggcgaag taaatgggat tatcgagaac caataagcaa
    15481 gacttccatt aggattttgc attgcagtaa gcataacaaa taatcccaag accattggca
    15541 gagtaattgg caacataaac tgctgtgtat cagcctcatt atcaaccgct gatcctacta
    15601 ttgcaaacat tgctccatac aataaatagc cgcctataaa ataaaataag aaacaagcaa
    15661 ttaccgcaaa ataatttaca gagtaaactg cttggaatat ttcttcaaat tccattgctt
    15721 tttcatctgg cacaacacta gtagcatcaa gttcacttcc catttccata actgttggat
    15781 ttaattttga agcatcataa tcttttaata caattgcctg acctgcactt actaaaccta
    15841 ttgttaacag cacccacaaa gcaaattgag taattcctac taatgcaaca ccaacaattt
    15901 ttcccatcat aagctggaat ggttttaccg aagacacaat aacctcaaca attcggttag
    15961 ttttttcttc tatcactcct ctcataactt gagttccata cataagtacg aacatgtaaa
    16021 ttaaaaatcc acttacatat ccaacaatca ttgccacttc agaatgggtt tctttaaact
    16081 cgcctttttc gtcaataata tttgtaacta cctgaatttc agaatttgcg acacttaaaa
    16141 tttcatctat ttgctcttga gtaatttgca aatcgtctgc agtaacttga aactttatgt
    16201 ttttaagttt attttctata ttatgtgcaa tatgcatttt tatacccaca ttaggttgag
    16261 tataagaata aaatattaca gaacctttta tattattaac cgcattttta ggaatatata
    16321 aaagtgcata ataatcagag tcttttaaat catctgtagc tttagtaaca tcgtatgtaa
    16381 aagttttttg atcaaaatat ctgcctgaaa aatcataaaa ctctatatat tctgtgttcg
    16441 tcaaagagtt tacaaataca cctgtttcgt caataacagc aattctttta aactcatcat
    16501 cttctaactg cataagaacc gctggaagaa tcattaaact tgcaaagagt ataggaccta
    16561 gtatggtcat tactataaaa gattttttct ttactcttgt ggtatattct cgttttatta
    16621 ttaatcctat tttgctcatt tttccaaatt atttaattag tgtgtttgtt tcttcaacaa
    16681 ctttaataaa aatatcgttc atgcttggca agtgtttttg aaaagaactt atattgccat
    16741 attgagtcat tatagtaatc aagtcgttag atttaatatt tttatttagc acttccactt
    16801 tcattgtaga tttttgacta ttttctacaa catcaagaat tttatagtct gatgttaaag
    16861 tgctttcaag tttgttgaaa tatccttcga aagtaatttc gtaaatatct gaagcataat
    16921 tatttctaat ttcatttatt ggtccttcca atatttttct tgacttgttt attagtgcta
    16981 tatggtcgca aatttcttct acagaagcca tattatgagt agaaaaaatt attgttgctc
    17041 cttcatttct tagatttaga atttcgtctt tcaacatttt agtatttatg gggtcgaaac
    17101 cactaaatgg ttcgtcaaaa attaaaagtt ttggtttatg caaaacagta attataaact
    17161 gtactttttg ttgcattcct ttagatagtt cctcaacttt tttatcccac caagattgga
    17221 tttcaaattt ctcgaaccaa tattttaatc tctttagtgt ttctgctttc gacaaaccct
    17281 ttagttggca caaatataga gcttgctcac ctactttcat ttttttatac aagcctcttt
    17341 cttcgggaag gtatccaata ttttctacat gtttactttg cattttttct ccataaataa
    17401 aaacttgccc tttatcgggt gctgtaattt gattaataat tcttatcagc gtagtttttc
    17461 ctgctccatt tggtcccaac aaaccatata cactaccttc tggaacagtt atggacacat
    17521 catctaaagc taaatgattt gcaaattttt tttcaacatt ttcagcaact agcgcattca
    17581 tttattttga ttttttatta aaattagaat ttgcaaattt aaaaagaatt ttttattaac
    17641 attaataatt gagaattaaa taattgagtt aaaagtaaaa agcgatgcac ttaacgtagt
    17701 tgaagtgtcg aaccgagctt gcgagctcac ctaaggtaaa tctaaaagca ttgtgttgtc
    17761 ctacggacaa gcctcgtata gtcctgtttt gtacttcgga caaataaaca cgtattagct
    17821 cgctcggatt tgtaatccaa gtgtaatact accaacactc ggattacaaa tccgagcgag
    17881 cgagagaagt cgcgggtgtg gcttaacttt acgaacttta aaaactaaaa aggtctataa
    17941 gtcaaaaagt aggctgtaat cttctaaaag tgtttctatg actagctttg tataaaatca
    18001 aagtttatga ataaaaaaag gctatcttaa aaatcagaca gccttttttg aaaaaattat
    18061 taaaaataaa tttttacata gtaataggac attcagctcc atttattgtt gctacgatat
    18121 ttgcttcatc acaggtgcta ccatcaaaat tcacagtaaa tggtaatatt cctgtaatac
    18181 ttatttttaa aactcctgct ctaacatatt tgcaacaagc taatatatct aatttttgtt
    18241 gtacagtaag agtatattca atactatcgg aactcatacc atcttgactt cctccaatca
    18301 aataattatc atcgcaaggg tcaaaaacag tattttctcc agctgcccat tcgcgacttg
    18361 tattttcatt aaagtatata attttgtctt gaggtgttgt gatttttcct tcagcaattg
    18421 taacattata aactaaatta ttattgtcgt ttctgccggc atttacaacc ttcatagttc
    18481 cttcaacttt atggtcgttg tggtaaaacc catcataagt aaccgtaatt tctgtacctg
    18541 cttcgcgata aaaacctgtg gtttggatat tgattattcc acggcgttta acaccatcgt
    18601 tgcaaacata atttgaagtt ccataatcta ctttaatatt tttgggccaa gtagttgtat
    18661 ctaatggcgt aattgtaact acaggataat cacctgcttg tccatatttt tgggcttcta
    18721 gttgtttgtc tccatttttt gcagctttat ctgcttctga aaatgaatcg gaaaaaattt
    18781 tgtttaccaa agcatattct gaaattgcat ttatagtttg gtcaatgcta ggtgcctgtt
    18841 gtataggttc cggcgttgga tcgtcagggt cgcaagaaaa tgcaaaaatc ccaagactta
    18901 aaattaataa ataaataatg tttttcttca taattttaat atttagtaaa taatttaatt
    18961 gtcaagtttc acaaggttgt ttatgataac ttgtattaat ttgtggtaaa
//
LOCUS       DAOPNX010000045        18934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_3536_length_18934_cov_5.710313, whole genome shotgun sequence.
ACCESSION   DAOPNX010000045 DAOPNX010000000
VERSION     DAOPNX010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18934
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3536_length_18934_cov_5.710313"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PKN53_06390"
     CDS             complement(<1..627)
                     /locus_tag="PKN53_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88612.1"
                     /translation="MKKYFTRIIFPFLILALIPFYGVKSQQVIEITGDGSDATHDMIP
                     VCGMWFGELQKSQIIYPAVKLTALQGKKITKITLFTNQGNNNKWKNNIHIRMASTTQN
                     DLESGWFIGSKTEVFVGKLLINSNNELNIVLSTPFFYTGDNLLIEFDLPNNINLDSES
                     VYFQGTTEATYVSRQVSGQIEYNGLGTAFDFLPKMRVEYADEACPPIIN"
     gene            complement(850..>1911)
                     /locus_tag="PKN53_06395"
     CDS             complement(850..>1911)
                     /locus_tag="PKN53_06395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD88613.1"
                     /translation="WPNACTEDLAFKIYNGSTQIGYSDANDLTSWTTNILPNGVYTLK
                     VVIFYYDNASNLLCSAEASTTATIDGGFDCPPVNNLSAIINEDNTAKLSWTNPEELPT
                     GCDGNLTFKFYIGNINVGSSNNDNLTSWTTPVLSEGTHNLAVVIHYLDNTNAEICYNQ
                     AYTTVTIYGNPTCPPINNLSATVNSDNTVTLSWTNPPQSEWASDEPYYWFYDDEIEIG
                     SDNTYNLNSWTTAELTEGVHSLGVQIGYYSTIAEDYICKADIIFVEVTITNVEEIKTS
                     LFSAEIFPNPAKDLLNIKCDSEIISYELYDALGRLLINKTEVANTESIVNVSSLKHGI
                     YMLKLNTQKGTGTFKIIKN"
     assembly_gap    1912..2011
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2012..>3762)
                     /locus_tag="PKN53_06400"
     CDS             complement(<2012..>3762)
                     /locus_tag="PKN53_06400"
                     /inference="COORDINATES: protein motif:HMM:NF019295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88614.1"
                     /translation="TNLTATSNPNNTVTLSWTNPPQSEWPSTDIDYFFYDGTTSIGYN
                     YSDELTSWTTPELTIGNHSLGVEAGYYSSGSWICKAEIIYVNIVVGEESCLPIAELST
                     TVNGNNVSLTWETPETPAIIFSEGFESGIPSNWTLLDADGNGHNWDLNTSGGENSNNC
                     VTSLSSSPNYLNTDNYLITPELEENVTKVRYSIRTPITYQEHYAIMASSTGNNASDFT
                     IVFEETVTTFANWHEKQVDLPPGTKYVAFRHYNSLNQWSLSIDNIVMYGNNAPITNPH
                     TYTITRNNMVVASEITATTYTDVGVATGVHDYCVKAVYPSCSSEKVCTNATVTCAPIT
                     NLSATTNDNNVILNWNTPNTLPEIILSEDFESGMPSNWTVIDANGDGLNWELNTTTGH
                     NSNNCVSSLSYNSNEGYSLYPDNYLITPLLNNVTKLQFWVSVGAYNPYEHYAVMVSTT
                     GNNINDFTSIFEETMTNSENWYKRSISLPSGTKYIAFRHYNSIDQSGLNIDDICIFGA
                     IIPTTELHTYTIARNGVEIASGLTGLTYTDSDLDYETYNYCIQAVYSSCTSETVCTNI
                     TVEEIYCLPVTNLNATV"
     assembly_gap    3763..3862
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3863..4681)
                     /locus_tag="PKN53_06405"
     CDS             complement(<3863..4681)
                     /locus_tag="PKN53_06405"
                     /inference="COORDINATES: protein motif:HMM:NF019295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOD88615.1"
                     /translation="MSKVFTKLFFPLCLLIAYSNITLAQTIILEEGFEGGSLPTGWSV
                     VDADGDGQNWSVTTSSTSHSGSYYIMSRSWNTNPLNPNNYLITPLVEGATNIKYYFKV
                     NINYPYEHYAVMASTTGNALSNFQIIFEETATATNNWTERNINLPAGTKYVAFRHYNS
                     TDQDYLALDDITIFSSNSACPPITNLTATVNPNNTVTLNWTNPPTSTWPSECDGDIYF
                     EFYNGSTEIGYDDNNDLTSWITNVLEVGTHSLGVTISYWKNGNQNVCYAETVFVD"
     gene            complement(4776..5657)
                     /locus_tag="PKN53_06410"
     CDS             complement(4776..5657)
                     /locus_tag="PKN53_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOD88616.1"
                     /translation="MRRVKLVFIVFILVCSNIFAQQAESRFRVIIDTDCAPDDLRAIC
                     LIFASNDFDVIAISTSDGLLSPEQGYKKIKAMLKHFGHEEIPVAYGKKSNKENKFCNK
                     LCLSVNWGDEQGIEIPEKPSAVELITKEIHNQKADIYLICFGPLTNISEVLKNENTHS
                     KIKQILWYQGSPNTGYNVNSDLEAAKFVTECKTVDKVFFNTPSTKQNLFTTKFLNKIS
                     KIKNPYSDFIFVSHQNLGVQEKIKAKFYKFWDDLLPIYLLNPELFDTTKENNIIYASP
                     KNKNFKNAYLQILNSKP"
     gene            complement(5884..7143)
                     /locus_tag="PKN53_06415"
     CDS             complement(5884..7143)
                     /locus_tag="PKN53_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HOD88617.1"
                     /translation="MEFSLNKEFFIRNRKNLFSLLPNNSMAILFPAEQFVRNGDALYK
                     YRQNSDLFYFSGLKQEKTVILLCKINNSTDEYAFIIEPNEKMLIWTGHKYSIDEAKEI
                     SGIKNVFYLNEFEKYFKENLNNDTLVYYSMSSNTRGFDYKNRIITEWKEGLKSDKIQF
                     KDLDPLSMQLRLQKQKEEIAIIQKAIDITGEAFKKVLDFITPGVYEYEVEAEISHHFI
                     KNGVESHAYSPIIAAGKNNCVLHYETNRELCKNGDLLLMDFGAEYNYYDADLSRTIPI
                     SGKFTNRQKQVYASVLKVLKEMKTKYVPGQTIRKLNKECELLIQEELLSLGLLSEADI
                     KNQNEDSPALKKYYMHGVSHFIGLDVHDVGVKDTVLLPGMVLSCEPGIYIAEEGFGIR
                     LENDILVAENPIDLCSNIPIEISEVCK"
     gene            7308..7769
                     /gene="bcp"
                     /locus_tag="PKN53_06420"
     CDS             7308..7769
                     /gene="bcp"
                     /locus_tag="PKN53_06420"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOD88618.1"
                     /translation="MKKLEIGDKAPDFKGLNQDGEEVKLSDFLGKNLILYFYPKDSTP
                     GCTAEACDLRDNYQMWQSKSYEILGVSPDSQKSHQKFIEKYNLPFDLISDTEKEILIA
                     YNAWGEKSMYGKKYMGVLRKTYVINSKGIIENIFEKVKTKEHSNQILNEIK"
     gene            7781..8785
                     /gene="recA"
                     /locus_tag="PKN53_06425"
     CDS             7781..8785
                     /gene="recA"
                     /locus_tag="PKN53_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOD88619.1"
                     /translation="MANTPEDIRNEKLKAVKLAMGKIEKDYGKGSIMMLGSQPVEEIP
                     VIKSGSIALDIALGVGGYPRGRVVEIYGPEASGKTTLAIHAIAEAQKAGGIAAFIDAE
                     HAFDRAYSEKLGVDVENLIISQPDNGEQALEIADNLIRSGAIDIIVIDSVAALTPRAE
                     IEGEMGDSKMGLQARLMSQALRKLTASINKTNTCCIFINQLREKIGILFGNPETTTGG
                     NALKFYSSIRIDIRRSGQLKDSDDVTGSRTKVKVVKNKVAPPFKKAEFDIIYGEGISR
                     VGEIIDLGVDLEIIKKSGSWFSYGETKLGQGRDSVKTILEDNPELMEEIENKIIEKLK
                     "
     gene            8787..9806
                     /locus_tag="PKN53_06430"
     CDS             8787..9806
                     /locus_tag="PKN53_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD88620.1"
                     /translation="MLKYFFILIVSLLFLSSCNNRSAKNSDDILNDTTLNLEQLDQLI
                     RKNPDNAELYLKRADFYLTKEKNIDKAILDMESALRADTTMQDAYIKLAEMYLAVANS
                     EMSKKVLERCLTRYPFNVKARIDLAKIYFYVQMYQEAMSEILKIEVNNLQNTDSYFVK
                     ALILNETFAYKDAIEALKKSIDYDNNNWEAYNLIGKIYAEQDDKLALDYYKTATRLFP
                     ENDEIQFNAAWVHQQFGEIQNALEFYDKAISIDSLYYEAYYNAGYIYASDLKDYFKAI
                     DYFSKAIECDSLSHKAWYNRGYSYEQLNKLQQAENDYRKALTIFPNYDPAVEGLNIVI
                     DKQMK"
     gene            9832..11943
                     /locus_tag="PKN53_06435"
     CDS             9832..11943
                     /locus_tag="PKN53_06435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOD88621.1"
                     /translation="MKTLNLILISLIIFMVSCTGKKKDGTQIDNDFVPNVELTQDEID
                     KGILTPEILWKFGRISDPQLSPDKKTIVYNITHYDVEKNRGFTYIYTIPAEGGEVKRL
                     TGGENAFFNPRWSKNGSKIIYLSTETENVQLWTMNVDGSDKTQISELDKSINSFKISP
                     NGDKILFMADVKLENNPQDIYPDLPNTNVIMAADLMYRHWNAWTDDSYSHIFVADFDE
                     KSLQNIYDIMKDEKFDAPMSPYFDDSEITWSTDGKKIAYTCKKMSGKDYAVSTDSDIY
                     LYDLETKSTTNLTEGMLGYDKYPVFSPDSKYLAWTSMETPGYEADKERLFIMNLETKE
                     KEYLTEHFDYGASNVVWPQVEGNYDNLYFISCFQATHQIFKINYKSREVVQITEGDHD
                     ITSIKWNGNGFIAEKMSIKSATEIFTFDQYGKETQITFTNKHIYDNIAVSDYKEVWVP
                     TTDGKEMLTWVILPPNFDETKEYPAILFCLGGPQSAMSQFFSYRWNFQIMAANGYVVI
                     APNRRGVIGFGSEWCKQISGDYGGQNMKDYMSAVNYVKKESYVDEDRIGAVGASYGGF
                     SVFWLAGHNQDKTFKAFISHCGMYNLESQYAATEEMFFVNYDLGGAYWEKDNKKAQSS
                     YANSPHKFVQNWNTPILIIAGGYDFRIPYTESLQAFNAAQLNGVESKLLIFPEETHFV
                     LKPQNSILWQREFKAWLDKYL"
     gene            complement(11950..13383)
                     /gene="rodA"
                     /locus_tag="PKN53_06440"
     CDS             complement(11950..13383)
                     /gene="rodA"
                     /locus_tag="PKN53_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOD88622.1"
                     /translation="MNRSRRGNILVNIDWITVAIYLILVFLGWINIYSATYNESHVSI
                     FDISQKYGKQLIWIGAAIIIAIFTLIIDSKFYSSFAYVLYAVGILLLLSVLFFGIEVN
                     ASKSWFQIGDFRIQPVEFTKITVCLALARLLSDENFSFNKLSDIIRTAIIFALPIILI
                     FLQNDTGSALMFFSFIFVLFRQGMNKVIFITSFALVVFFILALVIDLEILLIISLVLN
                     IIGFTILNKHSNQKQIVFYTSLALAIASYILHKYTNIFVDKTLTLVLINTAAIALLML
                     VLFIIKKNKTILLFLTTIIGITIFVLSVDFVFNNVLQPHQQNRINDLLGISSDPLGAG
                     YNVNQSKIAIGSGGLTGKGFLKGTQTKYNFVPEQSTDFIFCTIGEEWGFLGSSLVLFL
                     FCFLILRLIFLAERQRSVFSRIYGYGVVSILFFHVAINIGMTIGLAPVIGIPLPFFSY
                     GGSSLWAFTILLFIFIKLDSDRLQIFR"
     gene            complement(13376..15184)
                     /gene="mrdA"
                     /locus_tag="PKN53_06445"
     CDS             complement(13376..15184)
                     /gene="mrdA"
                     /locus_tag="PKN53_06445"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOD88623.1"
                     /translation="MSNWSNRKYIIGAIILIVFITIFIKLFYLQIFDETLKQSSDNNS
                     QRHVTVYPGRGLIYDRNSVLLVCNEAAYDLMLVPRNLDEFDTLSLCHDINISIEEFYE
                     KLEKCRKYSMYRPSIFHKQINSSQYAVLQEHLYKYPGFFVQARSLRKYNEGIAAHILG
                     DIGEIDAKTLENDNYYSGGDYIGKSGVEKSYEELLRGKKGVEIFLVDVRGNIQGSYYD
                     GKYDIPATPGKDIHLTIDIELQRYGELLMKNKVGSIVAIEPKSGEILALVSSPSYDPQ
                     LLVGRARGHNYDSLLNSPAKPLINRAVSSTYPPGSIFKIAIALVALQEGVIKTNSYFP
                     CDVSKVACHGHPPANSVARGIQYSCNPYFYFVFKNTIQRGFEKSIFKDSRIGLNLWKE
                     KIMTLGFNQALDIGLPGVNKGLIPDSDFYDKWYGKHRWAFSTIYSLSIGQGEVLVSPL
                     QMANFCAILANRGFYHNPHILKKIDNEDYVGENTKKIFTPFAKEYFEEAVKGMDYVVN
                     EEYGTGFYARLSDIRVCGKTGTVQNRGKDHSVFIAFAPKDQPQIALAVYVENAGFGGT
                     WAAPIARLMIEKHIRGYVNDSIFEKRIIETEILSNE"
     gene            complement(15186..15689)
                     /locus_tag="PKN53_06450"
     CDS             complement(15186..15689)
                     /locus_tag="PKN53_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88624.1"
                     /translation="MKNNLRYILLFIIPLIFQVLIFDKAYFWGYANVYIYILPILLLP
                     ITLNKHLVLLLGFALGFTVDIFNSTPGIHAAATVFMTYFRPLVLRIYAPHDSYEVNKN
                     LGIINYGFKWFFKYAISLILLHHTFLFIVDTWSFANFGYTILKIILSCFASLLFIIMG
                     HLLIMKD"
     gene            complement(15679..16494)
                     /gene="mreC"
                     /locus_tag="PKN53_06455"
     CDS             complement(15679..16494)
                     /gene="mreC"
                     /locus_tag="PKN53_06455"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOD88625.1"
                     /translation="MRNLINFIFKYSYTILFIILLVISFIFIFSNSPLQRVSYFNSAN
                     RFSGLVSEKWQGLDDYFKLKKINNSLLLENQYLRNNLEKYRNEKVPAIDNISFDYIAG
                     KVINISVNKSNNFICVDKGKKQGIKNEMAVIGPNGVVGTVVSVSENFSTVMPIINKKS
                     ALSVKLEKNNYFGSLTWDGNSPSEAILTEIPSYISLNKGDKVITTGFSLIFPEGIPVG
                     IVKDFTINESDKFYSITVSLFTDFHNLNHVYFLKNSFIDEFNTLESKNKENEK"
     gene            complement(16504..17526)
                     /locus_tag="PKN53_06460"
     CDS             complement(16504..17526)
                     /locus_tag="PKN53_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOD88626.1"
                     /translation="MGLFSFLQQEIAMDLGTANTILIHNDKIVVDEPSIVALDERTGK
                     LVAIGHEARKMHGKTHDGIKTVRPLQDGVIADFNAAELMIRGMIKMMNKRRSWFNPSL
                     KMVICIPSGSTEVELRAVRDSSEHAGGREVYMIYEPMAAAIGIGMDVEAPQGNMIVDI
                     GGGSTEVAVISLGGIVCNRSIRIAGDDFTEDIQEYMRRQHNIKIGDRTAEDIKINVGA
                     AITELDNPPPDYMVRGPHQMTALPIEIPVSYQEIALCLDKSIAKLETAIMNVLELTPP
                     ELYSDIFTKGVYLAGGGALLRGLDKRLTDKTNIPFYVAEDPLHAVARGTGIALKNVEN
                     FPFLIR"
     gene            complement(17711..18139)
                     /locus_tag="PKN53_06465"
     CDS             complement(17711..18139)
                     /locus_tag="PKN53_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88627.1"
                     /translation="MIRMKSLEMPRDSIIKMDFFYIPWQSRTYGSFDEDYVRESEYSS
                     FYSISDKKIIDEFVVSMNLLNVKEVYKCEGSYFCVKMVIDFYYASGEKKTILIDRDGI
                     IKHNNDCYCYNLHFMKKILDYVPEAKLPSYFEIMLQDTLK"
     gene            complement(18236..18370)
                     /locus_tag="PKN53_06470"
     CDS             complement(18236..18370)
                     /locus_tag="PKN53_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88628.1"
                     /translation="MGEAVRTSHDIKEHFKAIMPFSLEGPSQSELHKTEPEIESRPRY
                     "
     gene            complement(18391..>18934)
                     /locus_tag="PKN53_06475"
     CDS             complement(18391..>18934)
                     /locus_tag="PKN53_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88629.1"
                     /translation="DPLKEKYLNLTPYNYCANGPLKNIDLDGKDIYITDPNTNKSYLY
                     VPGETAINAAKDSFVETVAKTLDELTSSGKDKFDIVSTIVGDENINANIKLGSWDDRG
                     NTVSTELNGKIYGTIPWSPEGGVITKEGGRQSPATVLLHELGHYYFDAYDPTGELAKD
                     KFIDIEKKKLESMVHLLTDG"
BASE COUNT         6427 a         2933 c         2867 g         6507 t
ORIGIN      
        1 attgattata ggcgggcaag cttcatctgc atattcaact cgcatttttg gtaaaaagtc
       61 aaaagctgtg cctagaccat tgtattcgat ttgaccacta acttgtcttg aaacgtaagt
      121 agcttcagta gtcccttgaa aatagacact ttcactatct aaattaatat tattgggtaa
      181 gtcaaactct attaaaaggt tgtcccctgt atagaaaaat ggagtgctta atacaatatt
      241 taattcatta ttagagttaa taagtaattt gccaacaaat acttctgttt ttgagcctat
      301 aaaccagcct gattcaagat cgttttgtgt tgtgcttgcc attctaatat gaatattgtt
      361 cttccactta ttattattcc cttggttagt gaacaaagta atttttgtta tttttttacc
      421 ttgtaaggct gttagtttta ccgctgggta aattatttgg cttttttgta attcaccaaa
      481 ccacattcca catacgggta tcatatcgtg tgttgcatca gatccatcgc ctgtaatttc
      541 tataacttgt tgtgatttta ctccgtagaa tggtattaaa gctaatatca aaaaggggaa
      601 aattattctt gtaaaatact ttttcatatt acaaccaatt taaagttagt gcttttttga
      661 ttgtaaaaat aatgtttttt tctaaaataa tattatttta aataaattat tttgaaagtt
      721 aaaaaattga agaaagtatt tagaaattcc caattttaaa actaaatact taacactcaa
      781 aattcttaac tatcaattca attatcaggt gtcgagcgta gccgagacac aattcttaat
      841 tattcattct taattcttaa ttattttaaa agttccagtt cccttttgag tattaagttt
      901 tagcatatat attccatgtt ttagcgaaga aacatttaca atgctttcag tattagctac
      961 ttctgtttta tttatcaata atctgccaag tgcatcataa agttcgtagc taataatttc
     1021 gctatcgcat ttgatgttga gcaaatcttt tgcaggattt ggaaatattt cagcagagaa
     1081 aagagatgtt tttatttctt caacattagt tattgtaact tctacaaaaa taatatcagc
     1141 tttgcaaata taatcttcag ctatagtgct gtaataacct atttgtactc caagagagtg
     1201 aactccttcc gtaagttcag ctgttgtcca tgaatttaaa ttataagtat tatcgcttcc
     1261 aatttcaatt tcatcgtcat aaaaccaata gtatggttca tcactagccc attcactttg
     1321 tggtggatta gtccagctaa gagttacggt attgtctgag tttactgtag cactcaaatt
     1381 atttataggt ggacaagtag gattaccgta aatagttact gttgtatatg cttggttata
     1441 acaaatttct gcatttgtat tatctaaata atgtataaca acagctaaat tatgagtgcc
     1501 ttccgaaagt actggtgttg tccacgaagt tagattgtcg ttattgctag aacctacgtt
     1561 aatattacca atgtaaaatt taaaagtaag atttccatca caaccagttg gcaattcttc
     1621 tggatttgtc cagcttaatt ttgcagtatt atcttcgttt attattgcac taagattatt
     1681 tacaggcggg cagtcaaagc ctccgtcaat tgttgctgtt gtgcttgcct ctgctgagca
     1741 aagaagattg ctagcattat cataataaaa tataacaact tttagtgtat agactccatt
     1801 tggaagtata ttggttgtcc atgaagttaa atcgtttgca tcactataac ctatttgagt
     1861 agagccgttg taaattttaa aagcaagatc ttcggtgcaa gcgttgggcc annnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntttactgtt gcattaagat tagtaacagg
     2041 taagcaataa atttcttcta ctgtaatatt tgtgcaaact gtctcggatg tacagcttga
     2101 gtaaacagct tgtatgcaat aattatatgt ttcataatct aagtccgaat ctgtataagt
     2161 aagtcctgta agtccgcttg caatttcaac tccatttcta gcaatagtgt aggtatgaag
     2221 ttctgtagta gggatgatag ccccaaatat acatatatcg tcaatattta aaccactttg
     2281 gtcgatacta ttataatgtc ggaaagcaat atatttagtt cctgaaggta aagaaatgct
     2341 tcttttatac caattctccg aatttgtcat tgtctcttca aaaatgcttg taaaatcatt
     2401 aatgttattt cctgttgttg aaaccattac ggcataatgt tcataagggt tgtatgcccc
     2461 tacactaacc caaaattgca atttagttac gttgtttaat aatggtgtaa ttaaatagtt
     2521 gtcggggtaa agagaatagc cttcatttga gttgtatgat aaagaactta cgcaattatt
     2581 actattatgt cctgtagttg tatttaattc ccaattaagc ccatcgccat ttgcatcaat
     2641 cacagtccaa tttgatggca ttccagattc aaaatcttcg cttaatataa tttcaggcaa
     2701 agtattagga gtgttccaat ttaaaattac gttattgtcg tttgttgttg cactaaggtt
     2761 agtaataggt gcacaagtta ctgtagcgtt tgtacaaact ttttcagagc tacaacttgg
     2821 gtaaactgct tttacgcaat aatcatgcac accagttgct acgccaacat ctgtgtaagt
     2881 agttgctgta atttcgcttg ctactaccat attatttcta gtaatagtgt atgtgtgtgg
     2941 gtttgtaata ggagcattgt taccatacat tactatgttg tcaatactca aactccattg
     3001 atttaaagag ttgtaatgtc ggaaagcaac atattttgtt cctggaggca aatcaacttg
     3061 tttttcatgc caatttgcga aagttgttac tgtttcttca aaaactattg taaaatcact
     3121 agcattattt cctgttgatg aagccatgat tgcatagtgc tcttgataag ttatgggtgt
     3181 acgaatagaa tatcttactt tagtaacgtt ttcttctaat tctggtgtaa ttaaatagtt
     3241 gtcggtgttt aggtaattgg gcgaagacga tagagaagtt acacaattat tactattttc
     3301 tcctccagaa gtgtttaagt cccaattatg tccatttccg tctgcatcaa gtaatgtcca
     3361 atttgaaggt attccacttt caaaaccttc gctaaatatt attgcaggcg tttctggagt
     3421 ttcccaagtt aaacttacat tattaccatt tactgttgta ctaagctcag caataggtag
     3481 gcaactttct tctccaacaa ctatatttac ataaataatt tctgctttgc aaatccaaga
     3541 accagaactg taatagccag cctctacacc aagagaatga ttccctatcg taagctcagg
     3601 agttgtccat gatgttaatt cgtcagaata attataacct attgaagtag ttccgtcata
     3661 aaaaaaatag tctatatctg tgctgggcca ttcgctttgt ggtggattag tccagctaag
     3721 agttactgta ttgtttggat ttgatgttgc agtgaggttg gtnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnatctacaa agacagtttc ggcataacat acattttgat
     3901 taccattttt ccaataagaa attgtaactc caagcgaatg agttcctact tctaatacat
     3961 tcgttatcca agaagttaaa tcattgttat cgtcataccc gatctctgtt gatccattgt
     4021 aaaattcaaa atatatgtcc ccatcacatt cgctgggcca agtgcttgtt ggtgggtttg
     4081 tccagttaag tgttactgta ttgtttggat ttactgttgc agtgagattg gtaataggtg
     4141 gacaagcaga gttactgctg aaaatagtta tgtcgtctaa tgctaaatag tcttgatcgg
     4201 tactgttgta atgacgaaaa gcaacatact ttgttcctgc aggaagattt atattgcgtt
     4261 ctgtccaatt atttgtggca gtagctgttt cttcgaatat aatttgaaaa tttgaaagtg
     4321 cattacctgt tgttgaagcc attacagcat aatgttcgta agggtagttt atatttactt
     4381 taaagtagta tttaatattt gttgcacctt ctactaatgg tgtaattaaa taattgtttg
     4441 ggttaagtgg attagtattc caagatctag acataatata ataagaacca ctatgactag
     4501 ttgaactagt ggtaacactc caattttgtc catcgccatc tgcatcaaca actgaccagc
     4561 ctgtaggcaa tgaaccacct tcaaatcctt cttctaatat tattgtttgt gctaatgtga
     4621 tgttggaata tgctattaat aagcataatg ggaaaaacaa ttttgtaaaa actttgctca
     4681 tatttattta atcatttgaa gcaaaaataa taattaaaaa taacatacat gcaaaaattg
     4741 tgtcaaaaat aattttttaa agaagtttta ttttattatg gcttactgtt caaaatttgt
     4801 aaataagcat ttttaaaatt tttgttctta ggagaagcat aaataatatt gttttcttta
     4861 gtcgtgtcaa atagttcagg atttaataaa tatattggca ataaatcgtc ccaaaatttg
     4921 taaaatttag ctttaatctt ttcttgtact cctagattct gatgtgatac aaaaatgaaa
     4981 tcagaatatg ggtttttaat ttttgaaatt ttatttaaaa attttgttgt aaataaattt
     5041 tgcttagttg aaggggtgtt aaaaaatact ttatccacag ttttacattc tgtaacaaat
     5101 tttgctgctt ccaaatctga atttacgttg tatcctgtgt taggcgaacc ttgataccac
     5161 aaaatttgtt taatcttaga atgtgtgttt tcatttttca atacttctga aatatttgta
     5221 agcggaccaa agcaaattaa ataaatatct gctttttggt tgtgaatttc ttttgtaatt
     5281 agttcgactg ctgaaggttt ttcagggatt tctatacctt gttcatctcc ccaatttact
     5341 gataagcaaa gtttattgca aaatttattt tctttattcg attttttccc gtaagcaaca
     5401 ggtatttctt cgtgtccaaa atgttttagc atagctttta tttttttata tccctgttct
     5461 ggacttaaaa gcccatcgct tgtgctaatt gctataacat caaaatcatt ggaagcaaaa
     5521 ataaggcaaa tcgctcttaa atcatcggga gcacaatctg tatcaataat tacacgaaaa
     5581 cgagattcag cttgttgtgc aaaaatattt gaacaaacta atataaaaac tataaacact
     5641 aattttactc gtctcatatt cacaaatttt ttattcttaa tttattgtca tactaaaaac
     5701 tcaacactct tttaacttta ccttcggtga gctcgcaagc tcggttcaac ttttaacttt
     5761 taactgaagt aattcttcat tctcaattct tcattattaa ttcttaattc ttcattctta
     5821 attcttcatt ctcaattctt aattcttaat tctcaattct caattctcaa ttctcaatta
     5881 ttctcattta caaacctcac taatctcaat aggaatatta ctacacaaat ctataggatt
     5941 ttcagcaaca agaatatcgt tttccaatct aattccaaag ccttcttcag caatataaat
     6001 tcctggctcg caacttaaaa ccatacctgg caaaagaaca gtgtctttta ctcctacatc
     6061 atgtacatca agccctataa agtgcgaaac tccgtgcata taatattttt taagagcagg
     6121 agagtcttca ttttgatttt ttatgtcggc ttcgcttaat aatcctaagc ttaaaagttc
     6181 ttcttgtatt aaaagttcac attctttatt tagttttcta attgtttgac ctggcacata
     6241 tttagtcttc atttctttca aaacttttaa aaccgaagca tagacttgtt tttgcctatt
     6301 tgtgaatttt ccagatatag gaattgttct cgacaaatca gcatcgtaat aattatactc
     6361 agctccaaaa tccataagta gtaaatcgcc gtttttgcat aattctcgat ttgtttcgta
     6421 gtgtaaaaca caattattct ttccagctgc aattattggt gaataagcgt gtgattctac
     6481 tccattttta atgaaatgat gacttatttc tgcttcaact tcatattcat aaactcctgg
     6541 ggtaataaaa tctaaaactt ttttgaatgc ttcgccagta atatcaatag ctttttgaat
     6601 gatagcaatt tcttcttttt gcttttgcaa ccttaattgc attgataaag ggtctaaatc
     6661 tttaaactga attttgtcgc tttttaaacc ttctttccat tctgtaataa ttctgttttt
     6721 ataatcaaat cctcttgtgt tagacgacat tgaataatat actaaagtgt cgttgtttaa
     6781 attttcttta aaatattttt caaattcatt caaataaaaa acatttttaa ttcctgaaat
     6841 ttctttggct tcgtcaatag agtatttatg tccagtccag ataagcattt tttcgttggg
     6901 ttcaataata aaagcatatt catctgtcga attgttaatt ttacataata aaatcacagt
     6961 tttttcttgt ttcaaaccac taaaataaaa caaatcgctg ttttgacgat atttatacaa
     7021 tgcatctcca ttccttacaa actgttcggc aggaaaaaga attgccattg aattgtttgg
     7081 taaaagcgaa aagagatttt ttctgtttct gataaaaaat tctttattaa gtgaaaattc
     7141 catttttaaa aaagtttaaa gaactaaata tatgaatgca aatttacata ttcttatgca
     7201 aattattaaa ttttaagtaa atgttaataa actttttcaa ataaatttat ttttcccgat
     7261 tatgattttt atatttgtac cttgtagaat actaaaatgt taaaataatg aaaaaattag
     7321 agattggaga taaagctcct gattttaaag gacttaatca agatggggaa gaagttaaat
     7381 tatctgactt tttgggcaaa aacttaattt tatattttta tccaaaagat tctactccag
     7441 gttgtactgc tgaagcatgc gatttacgag ataattacca aatgtggcaa agtaagtctt
     7501 acgaaatttt aggagtaagt cctgatagtc aaaaatctca tcaaaaattt atcgaaaaat
     7561 ataatttacc atttgattta ataagcgata cagaaaaaga aattttaatt gcttataatg
     7621 cttggggcga aaaatctatg tatggaaaaa aatacatggg tgttttacga aaaacttatg
     7681 taattaattc aaagggaatt atagaaaaca tttttgaaaa agtaaaaaca aaagaacatt
     7741 caaatcaaat tcttaacgaa atcaaataaa atttaaaaat atggcaaata caccagaaga
     7801 tattagaaac gaaaaactca aagctgtaaa attggctatg ggaaaaatcg aaaaagatta
     7861 tggcaaaggc tctataatga tgcttggttc acaaccagta gaagaaattc cagttattaa
     7921 atcaggctct atagcattag atattgcttt gggagttggc ggttaccccc gcggtagagt
     7981 ggtcgaaatt tacggacccg aagcctcagg taaaaccact ttagctattc atgctatagc
     8041 agaagctcaa aaagctggcg gaatagccgc atttattgac gcagaacatg ctttcgatag
     8101 agcttattcc gaaaaacttg gagttgatgt agaaaattta ataatttctc aacccgacaa
     8161 tggagaacaa gctttagaaa tagcagataa tcttattcgc tcaggtgcta tcgacattat
     8221 agtaattgac tctgtggcag ctcttactcc acgtgccgaa atagaaggcg aaatgggcga
     8281 ttctaaaatg ggcttgcaag caagacttat gtcgcaagca ttacgtaaat taacagcaag
     8341 cattaacaaa acaaatacat gttgtatttt tataaatcaa cttcgcgaaa aaattggaat
     8401 tttattcgga aatccagaaa ctacaacagg aggtaatgct cttaaatttt actcatcaat
     8461 tcgtatagat atacgcagat cggggcaact taaagatagt gacgatgtta ctggaagcag
     8521 aacaaaagtg aaagttgtga aaaataaagt agctccacca tttaaaaaag ctgaatttga
     8581 cattatttat ggcgaaggaa ttagtagagt aggtgaaatt attgaccttg gagttgattt
     8641 agaaattatt aaaaaaagcg gttcttggtt tagctatgga gaaactaaac taggacaagg
     8701 aagagatagc gttaaaacta ttttagaaga caatccagaa ttaatggaag aaatcgaaaa
     8761 taaaattatt gaaaaactaa agtagaatgc taaaatattt ttttattcta attgtatcac
     8821 ttttattctt gtcttcgtgc aataatcgaa gtgctaaaaa tagcgatgat attttaaacg
     8881 atacaacatt aaatttagaa caattagatc aacttattcg taaaaatccc gataatgctg
     8941 agctttactt aaaaagagca gatttttatt tgacaaaaga aaaaaatatt gataaagcaa
     9001 ttttagatat ggaatctgct ttgcgagcag atacaacaat gcaagacgct tatataaaac
     9061 ttgcagaaat gtatttggcg gttgcaaatt cagaaatgtc taaaaaagtt ttagaacgtt
     9121 gcttaactcg atatccattt aatgttaaag caagaataga tttagcaaaa atttactttt
     9181 atgttcagat gtatcaagaa gcaatgtctg aaattttaaa aatagaagta aataatttgc
     9241 aaaatacaga ctcttacttt gttaaagcac ttattttgaa cgaaaccttt gcttataaag
     9301 atgctataga agctttgaaa aaatctatag actacgataa taacaattgg gaagcataca
     9361 atttgatagg aaagatttat gccgaacaag atgataaatt agctttagat tattataaaa
     9421 ctgcaacaag attattcccc gaaaatgatg aaattcaatt taatgcagct tgggttcatc
     9481 aacaatttgg agaaattcaa aatgccttgg agttttatga taaggcaata agtatagata
     9541 gcttgtatta cgaagcatat tataatgctg gatatattta tgcaagtgat ttaaaagatt
     9601 attttaaagc catagattat ttttcaaaag caattgaatg tgattctcta tctcataaag
     9661 cttggtacaa tagaggttat tcttatgaac aactaaataa attgcaacaa gctgaaaatg
     9721 attatagaaa ggctttaaca atatttccaa attacgaccc agcagttgaa ggacttaata
     9781 ttgtgataga taaacaaatg aaataaattt aaattaataa aactttatat tatgaaaaca
     9841 ctgaacttaa ttttgatttc gttaataatt tttatggttt cttgtactgg caagaaaaaa
     9901 gatggaacac aaatagacaa tgattttgtt cctaacgtag aacttactca agatgaaatt
     9961 gataaaggaa ttttaactcc agaaattttg tggaaatttg gaagaattag cgaccctcaa
    10021 ttaagtccag ataaaaaaac aatagtgtat aatattactc attatgatgt tgaaaaaaat
    10081 agaggtttta cttatatcta tacaatacct gccgaaggtg gcgaagtaaa acgtttgaca
    10141 ggtggagaaa atgccttttt taatccaaga tggagcaaaa atggaagtaa aataatatat
    10201 ctatcaaccg aaactgaaaa tgttcagctt tggacaatga atgttgacgg aagcgataaa
    10261 actcaaattt cggaattaga taaaagtata aattcattta aaatttcacc taacggcgat
    10321 aaaatattgt ttatggcaga tgtgaaatta gaaaataatc ctcaagatat ttatcctgat
    10381 ttgccaaata caaatgtaat tatggcagca gatttaatgt atagacattg gaatgcatgg
    10441 acagacgatt cttattctca tatttttgtt gctgattttg acgaaaaatc gcttcaaaat
    10501 atttacgata ttatgaaaga tgaaaaattt gatgcaccaa tgagtccata ttttgatgat
    10561 tctgaaataa cttggagtac agacggcaaa aaaatagcat atacttgtaa aaaaatgtcg
    10621 ggaaaagatt atgctgtaag tacagattca gatatttatc tttatgattt ggaaacaaaa
    10681 tcaacaacaa atttaaccga aggtatgttg ggttatgata aatatcctgt gttttctcca
    10741 gattcaaaat atttagcttg gacaagtatg gaaactcctg gttatgaggc tgataaagaa
    10801 agattgttta taatgaattt agaaaccaaa gaaaaagaat atttaaccga gcattttgat
    10861 tatggtgcaa gcaatgtagt ttggcctcaa gtagaaggaa attatgataa tttgtatttt
    10921 atttcttgtt tccaagctac tcatcaaatt tttaaaatta attataaatc tcgcgaagta
    10981 gttcaaatta ccgaaggcga ccatgatatt acatctataa agtggaatgg aaatggattt
    11041 attgccgaaa aaatgtcgat aaaatcagct actgaaattt ttacattcga ccaatatggt
    11101 aaagaaactc aaatcacttt cacaaataaa catatttatg acaatattgc tgttagcgac
    11161 tataaagaag tttgggttcc aacaacagat ggcaaagaaa tgttaacatg ggtaatttta
    11221 ccgcctaatt ttgatgaaac aaaagaatat cctgctattt tattttgctt aggtggacca
    11281 caatcggcta tgtcgcagtt tttctcatac agatggaatt tccaaattat ggctgccaat
    11341 ggatatgttg ttattgcacc aaatagaaga ggagtaatag gtttcggtag cgaatggtgt
    11401 aaacaaattt ctggcgatta cggtggacaa aatatgaaag attatatgtc ggctgtaaat
    11461 tatgtgaaaa aagaatctta tgttgatgaa gatagaatag gagcagtagg agcaagttat
    11521 ggcggttttt ctgtattttg gctcgcagga cataaccaag ataaaacttt taaagccttt
    11581 atttctcatt gtggaatgta taatttagaa tctcaatatg ctgcaactga agaaatgttc
    11641 tttgtgaatt atgatcttgg tggagcttat tgggaaaaag ataataaaaa ggctcaaagt
    11701 tcttatgcaa actcgcctca taaatttgtt caaaattgga atactcctat tttaataatt
    11761 gctggcggat acgactttag aattccttat acagaaagtt tacaagcatt taatgcagca
    11821 caacttaatg gtgttgaatc aaaactcttg attttcccag aggaaactca ttttgtgcta
    11881 aaacctcaaa atagtatttt gtggcaaaga gaatttaaag cttggttaga taaatatttg
    11941 tagaatattt tatctaaaaa tttgtaatct atctgaatct aactttataa aaataaataa
    12001 aagtattgta aaagcccaaa gcgaagaacc accataacta aaaaatggta aaggtattcc
    12061 aattactggg gctaaaccaa ttgtcattcc aatgtttatg gcaacatgga aaaataaaat
    12121 tgagactact ccatagccat aaattctact aaaaactgac ctctgtcgtt cggctagaaa
    12181 aattaatctt aaaattaaaa agcaaaataa aaacaaaacc aaactagaac ctaaaaagcc
    12241 ccattcttcg cctatagtac aaaaaataaa gtcagtgctt tgttctggca caaaattata
    12301 ttttgtttga gttcctttta aaaagccttt gcctgtcaaa ccaccagaac ctattgcaat
    12361 ttttgattgg tttacattat atccagcacc taaagggtca gagcttatgc ctaataaatc
    12421 attaattcta ttttgttgat gaggctgtaa aacattgttg aaaacaaaat caactgataa
    12481 aacaaaaatt gtaatgccaa ttatggtggt aagaaataaa agtatagtct tatttttttt
    12541 aattataaat aaaactaaca ttaacaatgc aatagctgct gtgtttatca atactagagt
    12601 taaagttttg tcaacaaaaa tatttgtgta tttgtgtaaa atgtatgatg cgattgcaag
    12661 tgcaagagaa gtgtaaaaaa ctatttgctt ttgattagaa tgtttgttta gtattgtgaa
    12721 accaataatg tttaagacta aacttattat taataaaatt tcaagatcta ttactaaagc
    12781 taaaataaaa aatacaacta atgcaaacga ggtaataaaa ataactttat tcattccttg
    12841 acggaataat acaaaaataa atgagaaaaa cattaaagca gaaccagtat cgttttgcag
    12901 aaaaataaga ataataggta aagcaaaaat aatagcagtt ctaattatat cgctcaattt
    12961 attgaaacta aaattttcat cactaagtag tcgggcgagg gctaagcata cagtaatttt
    13021 agtgaactca acaggttgaa ttctaaaatc gcctatttgg aaccaagatt ttgacgcatt
    13081 aacttctatt ccaaaaaaca aaacacttag taaaagtaaa attcctactg catataaaac
    13141 ataggcgaat gatgagtaaa atttactgtc aattattaaa gtaaaaatgg caataatgat
    13201 agctgcacca atccaaataa gttgttttcc gtatttttga gaaatatcaa aaatgcttac
    13261 atgagattcg ttgtaagttg cagagtaaat atttatccat cctaaaaata ctagaataag
    13321 gtatattgca acagtaatcc agtctatatt aactaaaata tttcctcttc tacttctatt
    13381 cattacttaa aatttctgtt tcaataattc ttttttcaaa aatactatcg tttacatatc
    13441 ctctgatatg tttttctatc attagtcttg ctattggagc tgcccaagtt cctccaaatc
    13501 cagcattttc gacatatact gccagagcaa tttggggctg gtcttttgga gcaaaggcta
    13561 taaatactga atggtctttt cctctgttct gcacagttcc tgttttgcca caaaccctaa
    13621 tatcacttaa tcttgcgtag aagcctgtgc catattcctc atttacaaca taatccattc
    13681 cttttactgc ttcttcaaaa tattctttag caaatggagt aaatattttt tttgtatttt
    13741 ctccaacata atcttcattg tctatttttt tcaagatatg cggattgtga taaaagcctc
    13801 tgtttgctag tattgcacaa aaatttgcca tttgtaaagg cgaaaccaat acttctcctt
    13861 gacctatact tagagaataa atagtagaaa aagcccatct atgttttcca taccatttgt
    13921 cgtaaaaatc agaatctgga attaagcctt tgtttactcc gggcaaacca atatctaatg
    13981 cctgattaaa acctaaggtc ataatttttt ctttccataa atttaagcct attcgcgaat
    14041 ctttgaaaat gcttttttca aatcctcttt gtatagtatt tttaaaaaca aaataaaaat
    14101 atggattaca agaatattga attcctctag caacgctatt agctggagga tgtccgtgac
    14161 aagcaacttt tgaaacatca catgggaaat atgaattagt tttaattaca ccttcttgta
    14221 gagcaacgag agcaatagca attttaaaaa tagaaccagg tggataagtt gaagaaactg
    14281 ctctatttat caatggtttt gcaggagagt ttaaaagact gtcataatta tgtcctcttg
    14341 ctcgtcctac aagcaattgg gggtcgtagc ttggactgga aactaaagct aaaatttcgc
    14401 cacttttggg ttctatagca acaatacttc ctactttatt tttcatcaaa agttcgccat
    14461 agcgttgcaa ttcaatgtcg atagttaaat gtatgtcttt accgggagta gcaggaatat
    14521 catattttcc atcataatac gaaccttgaa tatttcctct aacgtcaacc aaaaatattt
    14581 caactccttt ttttcctctg agcaattctt cgtaagattt ttcaactcca ctttttccaa
    14641 tataatcacc accactatag taattgtcat tttcaagagt ttttgcatct atttcgccaa
    14701 tatctccaag tatgtgagca gcaatgcctt cgttatattt tctcaaacta cgcgcttgaa
    14761 caaaaaatcc aggatattta tacaaatgct cttgtaatac tgcatattgc gaagaattaa
    14821 tctgtttatg aaaaattgaa ggacgataca ttgaatattt tctacatttt tctaattttt
    14881 cataaaactc ttcaatgcta atattaatgt catggcataa actcaaagtg tcaaattcat
    14941 ctaaattgcg aggaactaac attaagtcgt atgcagcctc gttacaaacc aataaaacag
    15001 aatttctatc ataaattaaa ccgcgtcctg gataaacggt aacatgtctt tgggaattgt
    15061 tgtctgaaga ttgttttaga gtttcgtcaa aaatttgaag gtaaaatagt tttataaaaa
    15121 tagtaataaa aactattaaa attattgcac caataatata tttcctatta ctccaattgc
    15181 tcatgtcaat ctttcattat taataaatgc cccataataa taaataataa tgatgcaaag
    15241 caacttaaaa ttatttttaa aattgtataa ccgaaattag caaaactcca agtatcaaca
    15301 ataaataaaa aagtatgatg taataaaatt aaactaatag catatttgaa aaaccatttg
    15361 aaaccataat ttataattcc tagattttta ttaacttcat aagaatcgtg gggagcataa
    15421 attcttaaaa ctaaaggtct gaagtaggtc ataaatactg ttgcagctgc gtgaatgccg
    15481 ggtgtggaat tgaaaatgtc aacagtgaat ccaagtgcaa atccaagtag taaaactaag
    15541 tgtttgttta gagttattgg taataataag atgggtaaaa tgtaaatata aacattggca
    15601 tatccccaaa aatatgcttt gtcaaatatt aaaacttgaa aaataagagg tatgataaat
    15661 aataatatgt atcttaaatt atttttcatt ttctttgttt ttactttcta aagtgttaaa
    15721 ttcatcaata aaactgtttt ttaaaaaata aacatgattt aaattatgaa aatcagtgaa
    15781 caagctaaca gttattgaat agaatttatc agattcgttt attgtaaagt ctttaacaat
    15841 tccaacaggt attccttctg gaaatattaa agaaaaacct gtggtaataa ctttgtcgcc
    15901 tttatttaat gatatatacg aagggatttc ggttaaaatt gcttctgagg gagagttgcc
    15961 gtcccaagtt aaagaaccaa aatagttgtt tttttctaat tttacactca aagccgattt
    16021 tttattaatg attggcataa cagtagaaaa attctcgctt acactaacaa cagttccaac
    16081 aactccgtta ggaccaatta ctgccatttc atttttaata ccttgttttt tgcctttgtc
    16141 aacgcagata aaattatttg acttgtttac cgaaatattt attacttttc cagcaatata
    16201 gtcgaagcta atattgtcta tagcaggaac tttttcattt ctatattttt cgagattgtt
    16261 tctaagatat tgattttcaa gcaaaagaga attgttgatt ttttttaatt tgaagtaatc
    16321 gtctaagcct tgccattttt ctgaaacaag tccactaaat ctattagctg aattaaaata
    16381 gcttactctt tgtagaggag aattagaaaa aataaaaata aaagaaatta ccaaaagtat
    16441 gataaataga atagtataag aatatttaaa aataaaattt attaaatttc tcatttttca
    16501 tttttatcgt attaaaaacg gaaagttttc aacattttta agagctattc cagtacctct
    16561 tgcaactgca tgtagagggt cttctgcaac ataaaatggg atatttgttt tatccgttaa
    16621 tcttttgtca agacctctaa gtaaggcacc tccaccagcc aaatatactc cttttgtaaa
    16681 aatatctgag tatagttcag ggggtgttag ttctaaaacg ttcataatag cagtttctaa
    16741 tttagctatt gatttatcta agcaaagagc aatttcttgg tatgaaactg gaatttctat
    16801 tggtagagca gtcatttggt gtggacctct aaccatatag tcaggtggtg gattgtcgag
    16861 ttcggtaatt gcagcaccaa cattaatttt tatatcttca gcagttctat caccaatttt
    16921 aatattgtgt tgtcgtctca tgtattcttg aatgtcttct gtaaagtcgt ctccagcaat
    16981 acgtatagag cggttgcaaa caattccacc taaagaaatt acagcaactt ctgtagaacc
    17041 accacctatg tcaacaatca tatttccttg aggagcttca acgtccattc caataccaat
    17101 agcagctgcc atgggttcat aaatcatata aacttcacgt ccgccagcat gttccgaaga
    17161 gtctctaaca gcacgaagtt caacttctgt agagcctgat ggaatacaaa taaccatttt
    17221 taaagaagga ttaaaccacg aacgcctttt attcatcatt ttaatcatac cgcgaatcat
    17281 taattcagca gcattaaaat ctgcaataac tccatcttgt aaaggacgaa cagtttttat
    17341 accatcatgt gtttttccat gcattttgcg ggcttcatga ccaattgcta ctaattttcc
    17401 agttctttca tctaaggcta caatagaagg ctcgtcaaca actattttgt cgttgtgtat
    17461 caaaatggtg ttggcagtgc ctaagtccat tgctatttct tgttgtaaaa acgaaaacaa
    17521 acccataaaa ataattttta attaattgtg caagataagt gtatttttga taaattgcaa
    17581 acactatcta cctaaaatgc aaagttatat tatttttttt aactgagaat taaaaattaa
    17641 actaataatt aatttaatag aaaaaaatga taatttattt ttcgaaagac tccacattgt
    17701 gctccacctg ctacttcaat gtgtcttgta gcatgatttc aaagtaagaa gggagttttg
    17761 cttctggcac ataatctagt atttttttca taaaatgcaa attgtagcaa taacaatcat
    17821 tattgtgttt tattatcccg tctctatcaa ttagaatggt ttttttttca cctgatgcat
    17881 agtaaaagtc tattaccatt tttacacaaa aatagcttcc ctcacatttg taaacttctt
    17941 taacatttag caagttcatt gatacaacaa attcatcaat aattttctta tcacttatac
    18001 tataaaatga gctatattca ctctctctta cataatcttc atcaaaacta ccataagtcc
    18061 tcgattgcca cggtatatag aaaaagtcca ttttaattat actatctctt ggcatctcta
    18121 aagatttcat tctaatcata taaatcctac tctgtttctg tgaaaaacca gtgaaataca
    18181 caaataataa cgctaataat aaaaataatc tatacataac ataaatattt aaaaattaat
    18241 atcttggtct tgattctatt tctggctcag ttttgtgtag ctcactttgt gagggtcctt
    18301 ctaaagaaaa gggcattatt gctttaaaat gctctttaat atcatgagaa gttcttacag
    18361 cctctcccat agctggttca actttctgta ttagccatct gtcagcaaat gaaccatact
    18421 ctccaacttc tttttttcaa tgtctataaa cttatctttt gcaagttctc cagttgggtc
    18481 ataggcatca aaataataat gtcctagttc atgtagcaat accgtagccg gactttgtct
    18541 tcctccttct tttgttataa ctcctccttc ggggctccat ggaatagtac catatatttt
    18601 gccattaagc tcagtactga ctgtatttcc tcgatcatcc catgagccaa gttttatatt
    18661 agcatttata ttttcgtctc caactatagt actaactata tcaaatttat ctttccccga
    18721 agatgttaat tcatctaatg tttttgcaac agtttcaaca aaactatctt tagcagcatt
    18781 aatagcagtt tcaccaggaa catataaata tgatttattt gtgtttgggt cggtaatata
    18841 aatatcttta ccatcaaggt ctatattttt taatggtcca ttcgcacaat aattatacgg
    18901 tgtcaaattc aaatatttct cctttaatgg atcc
//
LOCUS       DAOPNX010000046        18604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_3606_length_18604_cov_6.793250, whole genome shotgun sequence.
ACCESSION   DAOPNX010000046 DAOPNX010000000
VERSION     DAOPNX010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18604
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3606_length_18604_cov_6.793250"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PKN53_06480"
     CDS             <1..196
                     /locus_tag="PKN53_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HOD88630.1"
                     /translation="ITTEQGIFYSAEIELNDSILKTNYNKIIPFSQNMTGTAEIITDD
                     LRLSERFLNPLRSIWKKSVE"
     gene            complement(219..734)
                     /locus_tag="PKN53_06485"
     CDS             complement(219..734)
                     /locus_tag="PKN53_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOD88631.1"
                     /translation="MANILKAEFFNRDVLEVAPDLIGKILVRKFDDGSELRLKIKETE
                     AYKGEEDKACHASKGKTPRTETMYLEAGTIYIYLIYGMYYMLNFVTSKEDVPQAVLIR
                     AAGDYNGPGKLTKALKIDKNFNKQNIATCPNLWVEDDSKKIKLKTSTRIGIDYAGEFW
                     ANKQWRWILVE"
     gene            complement(756..1223)
                     /locus_tag="PKN53_06490"
     CDS             complement(756..1223)
                     /locus_tag="PKN53_06490"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOD88632.1"
                     /translation="MTKKDFMKLAIDLSIENIDKGGGPFGAVIVKDNKVIAASGNSVT
                     NDLDPTAHAEVNAIRKAAKILGNFDLSGCEIYASCEPCPMCLGAIYWARIDKLYFANT
                     KVDADKIGFSDKFIYDEFEKKVSERDLKTENFMREEALEAFRKWENSQTKTEY"
     gene            complement(1228..2796)
                     /locus_tag="PKN53_06495"
     CDS             complement(1228..2796)
                     /locus_tag="PKN53_06495"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOD88633.1"
                     /translation="MREKIHRYIYIFGLGVIAFFMPISEFLTNACIIMLSTNYLIEAN
                     FKQKIKTISKNKELLFFLLLFFIPLIWFFNSQNMEFALRDIKIRLPLLALPIIIASSK
                     ALTNKQFKTIVFLFIAGVLTSTLAGLLAYLGIYFELKSDNVRHYSVFISHITLSVMMS
                     LAILLLTYFIIKRKFSKLIYYLIAIAIIFWLMLFFALIQGFTGLGVLIVTSICVLAYK
                     MFTHKNKKIKILSFSVLILLIGSIAIYLSVQIKDFYTPSNPDIQLKECSIYGNPYVSD
                     TTVYLLENGNFIYYNVCEKELRVAWNNRSLMNFDGYDAKGQLLLHTIVRYMASKGLDK
                     DAEGFKELTDKDIVNIEAGHTNYRFVDKRGISQRVYNIIWQIDVYLKGVPPDGHSITQ
                     RFEFQKYGLILAKRNLLLGTGTGDLDDEYKAIYEEFDSKINIQNRHRAHNQFLTFYIT
                     FGIIGASFCMLAWFYPIFTNFRSRSPYFILFFLIASFSMFADDMLETSSAVVFISFFY
                     SLFLWGIEQVKEKN"
     gene            complement(2815..4272)
                     /locus_tag="PKN53_06500"
     CDS             complement(2815..4272)
                     /locus_tag="PKN53_06500"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOD88634.1"
                     /translation="MKKHLIILAFVSLFSSVNAQQALTFYNMDRVMQSQFVNPAAEID
                     YKFQMGGLILPLFGEMPPAMYFNYANNSIYYNHIFHKGEGSKADSLVLDLPLMMKKLN
                     KTTHFRFDTQFELLNLGISLQNMYLTISLTEKLKYGFSLPYDLFEFAINGNIPYMKEG
                     KPHDFKGFGLNFTHYKEFAVGMSMKANEELRLGGRMKILFGQSNFNTDIKKLSIHTDP
                     ENYMMTWVTDMKIQTSSPLNYEYIMTPDSIHLGIDEEEFDKLQNQGAGAMASAFLLNM
                     KNIGIGFDLGANYKINPEIEVYGSLNDFGFINWSNNPHNFMSEGEYDFRGIEFKIFDK
                     NENKFDESVDNLMDTLINTFKFDLLETSYITWLPSNLYLGGKYKFHEKLHFNVLYRGE
                     FYRKSYLQSLTIGASSNITNWLAAYLTWSYANNSINNIGFGLNVRAAIFNWYIVTDSF
                     TNMMFPQKAKNFNIRMGCNMTFGYKKIRSSASMEM"
     gene            complement(4297..5967)
                     /locus_tag="PKN53_06505"
     CDS             complement(4297..5967)
                     /locus_tag="PKN53_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88635.1"
                     /translation="MKTRICYLLIFFILFLSFSSCIKDNFDFDKWDKEINYEAGFAAP
                     AVWGNLSFTDALERFDSTGLLIKNDEGYVSMQYKTRVSSAAVQEIIYLNDQTASGSIP
                     SVIFNFSGFDQFGDTISHTISQNVPFTMFNPEAEIDSILLKSGIADIRTTSTYGHSAK
                     LYVTFPSITKNGQAFTRTFTYVPGGGSVHSLGNDFAGYKIDLTQTAANFNEIPIELKL
                     TLFFSGNGVPNTGSIDFSIDMKDMTYKTMHGYFGLNTLFFESDTINISLFNTDDWTIE
                     RYRFEDPKLEVFYTNSYGVPSQFYFTHMMAASAVDGTEYSIIDYGVGLPIGESNPYDI
                     SYSTNYGDVMIDSLKLSRNNSNINDIVNKRPKWIQFKAKATTNPAGNSHNNFVADNSK
                     IDVEVVMEFPLWGYIYNFDLRDTLDIDLTDLFSEDNPITRALLRTEFNNGFPVEAISQ
                     IYITDKYYNILDSVFTAGPEQLIGPAKVDANGKVTDFTQKITKIEYDLDRLEKVRGGK
                     YIILRVHGYTTDAANDKVVKIYTDYQIKFDIGFEVDVKYDGPIDSIGK"
     gene            complement(5972..8707)
                     /locus_tag="PKN53_06510"
     CDS             complement(5972..8707)
                     /locus_tag="PKN53_06510"
                     /inference="COORDINATES: protein motif:HMM:NF023260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HOD88636.1"
                     /translation="MKKRVLIISIIVVLCGLLVFAVLFFLKSSEKTSKALDAVPGNPA
                     LIIETNSFNSFFEKLSLDYVISYEIQKFFQVNSSNSLAHCFDSISKSDFFKEVKNRKS
                     LFLIDNSLSSLSQTLYIEIDEKAKTKQFQTNLNNELKQFSNINERDYNGNKIFDCSLQ
                     NGKKFFYSISNHILIISNSAVVVEKSIDALASNNSIIKRDSEFAALYESAGKKELANV
                     YINLPQLKSILKQNLAEDFDSKHEFANWIEMDLNINTGKLSMNGFIENKDSLPALSNI
                     LKQQSPCYQSCLDILPDNACYYTILAFTDSENYNNSLNSYLKSIGELDKREEAINELK
                     TTYNIEFKDFYKLIENEICISAIVENNKLDFYTILNLNSQSVGIFELENIIQNYASKL
                     NKNIKDFKSDLKVDEHTNLACYKLPADNLPLILFGPLFTKSNSSYVCFIKNFMVFAET
                     KEGLRNFAKTAFLNKTMTTNIEHNKFLENFSNKSLLFTYFSLTNGFPLIKDYLNSDLQ
                     NLAQSKFTELQKFSFWAYQINNASDRLYNNMILQYADNISEKPKTSWESRIDAPVSTK
                     PFIVTNHDDNSTEILVQDENNNLYLINCSGLEIWKIQLDESIFGNVYQIDAFKNGKLQ
                     YLFSTKSKIYLIDRLGNNVKNFPINLRSKASSPLSVFDYENNRNYRIAIACEDKKIYL
                     YDETGKIVKGWNFNNSENLVSSEISHYTIDNKDYLVFHDDFKFYSLNRRGEPRNTYFT
                     NFKFSKNNKVYLDKNKGKSQFVCTDAKGNVRYFSPQGQQDSLFIKEFSENHYFVVADI
                     DYDGNSDYIFADSTKLEVYNNNKKLLFELNFNDKISFEPSIYKFSNNQIKIGIVCRES
                     EKIYLINENGSIFEGFPLIGKSPFSIGFFNANDEKMSLTVGGLENLLYNYKLIKR"
     gene            8881..9555
                     /locus_tag="PKN53_06515"
     CDS             8881..9555
                     /locus_tag="PKN53_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOD88637.1"
                     /translation="MAKIIIFVSLFIFSKALFCNIDGDGASRVSACESVSYIDRVNSS
                     LSSFYDSTNIWWASQSVISDSLRKVNCPYYPEYVYNSRIADLDEKTPIKLEYNEHVLK
                     YIEAYGVKNRPKLQSIISKSAYYFPIFEEYLDKYGLPLELKYLAAVESALDARAVSKS
                     GAVGLWQFMKPTSDLLDVKVTSYMMSVEMYINQLMQRVGILNIYTKLLEIGNLLLLLI
                     MVVLEL"
     gene            9552..10145
                     /locus_tag="PKN53_06520"
     CDS             9552..10145
                     /locus_tag="PKN53_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOD88638.1"
                     /translation="MKKAIAQSGGKTNYWDLRPYFTEQMRNYVPAFIAMNYLMEYHAE
                     HNIFPNDVFIESVKTDTLYVNGYVHLKNIAELVGTDYELLKNLNPVYTYAYIPMDGDK
                     HCLVLPFDVVHKYYQKEQEIVALKNNVKNDTVLTQPPFMQKPVRENIIYHNVERGDNL
                     FRISQRYACTIPEIYQWNNLDDNYILRVGDSLKIMIE"
     gene            10229..11860
                     /locus_tag="PKN53_06525"
     CDS             10229..11860
                     /locus_tag="PKN53_06525"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HOD88639.1"
                     /translation="MFKFLRKENTIAGPKFNRWLVPPAALAIHLSIGMAYGFSVFWLP
                     LSKSIGITEAVLAPEGISFIERVFSTAYDWPISMLGWLYTLFFVFLGLSAAMWGGWLE
                     KVGPRKVGFISAILWSGGLLISALGVVLHQIWMLWLGSGVIGGIGLGLGYISPVSTLV
                     KWFPDKRGMATGMAIMGFGGGAMIGAPLAQKLMNFYATDTSVGVWETFVTLAIIYFIY
                     MIIGALSYRIPPTGWQPKGWKPKEKKRKSLITQHHVHVNKAWKTPQFWMLWLVLCLNV
                     SAGIGVIGMASPLLQEVFGGKLIGIDKVFSELNEAELAKIALVAAGFTGLLSLFNIGG
                     RFVWASLSDVIGRKTTFAIFFILGFILYVLAPHLGLAGTVALFVVAFCIIISMYGGGF
                     ATIPAYLADLFGTQMVGAIHGRLLTAWSTAGILGPVLVNYIREFQLNRGVPVQQVYNT
                     TFYILAGLLIIGMIANFLVRPVNKKYHMSEEELAKLRLATTKEEKNGNTNVDLSEVGS
                     AKATWKLIFAWTAVGIPLLYGIYSTVLDALPLFLR"
     gene            11957..12259
                     /locus_tag="PKN53_06530"
     CDS             11957..12259
                     /locus_tag="PKN53_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88640.1"
                     /translation="MANRRELKKDINWLTEEVVSDCLIYWEFNKNKNKEHVVDIINSV
                     ITKRNELISRINKPTSKMTRHEVKSAYSQVVKEMFEVTNESLEKLSELAKKNAKAN"
     gene            12243..12986
                     /locus_tag="PKN53_06535"
     CDS             12243..12986
                     /locus_tag="PKN53_06535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOD88641.1"
                     /translation="MQRLINIPEIGNIVVTKKTNAKNYRLRVHPQKGVLLTIPRFGNF
                     KEGENFVLKNIDWIKSKLKEREIRQQNLKIFKPGEEFITRNLSLEFFEGNFKNMLSAY
                     FENKKIKILYQTGFNDFEADDVQEFIKKFILKCLQQEANIYLIPRLNELSQKTGLKFK
                     NCKIGTASTRLGSCDSKNNIILSAYLMLLPDRLIDYIILHELCHILHKNHGEKFHNLL
                     NLLTNGKSKELNKELKSFRTSIEPGNYKF"
     gene            complement(12988..13551)
                     /gene="efp"
                     /locus_tag="PKN53_06540"
     CDS             complement(12988..13551)
                     /gene="efp"
                     /locus_tag="PKN53_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOD88642.1"
                     /translation="MATTADIKNGLTIEFNGKPYQITYFQHVKPGKGAAFVRTKMKSL
                     ENGRVIENTFPSGAKIDIIRIERRPYQFLYNDDLGYHFMHLETFEQVCLSEDLIEGIE
                     FLKEGQEVEVIFHAENEAALTCELPAFIISEVTYTEPGLKGDTTTNALKPATIDTGAS
                     IMVPLFINIGEKIKVDTRTKEYVERAK"
     gene            13731..14765
                     /locus_tag="PKN53_06545"
     CDS             13731..14765
                     /locus_tag="PKN53_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88643.1"
                     /translation="MKKILMLTFIAFIAFACKNSAQPETQEQQKKEEIKFEWASYENS
                     RFDFSVSYPDFLIPQGESGNGDGQRFISEDEKFQFWAYVDFKMNATFDDAPSFDEAYE
                     QDVNQENVEYNEKRQDNYYVSGHIDKNTLFSKYTYFRNMNYYVLYFQFPDAEARFFSP
                     IVDTIVASFDCDGGENHDVENFINGFLKDCWIGKNFDALLRDNSAILNKYIDKDLGIA
                     RLHAPGTIPRFAKRNENFGYDNYTDFETTFKGKYTLTMCKENYDLPCNFDFEDKNGKI
                     VCYDFVSDLPNLVDPETFEISSLTPMYPSSKIMLVYLPNKYNSPRAFYFILVNKNWKL
                     IIVDDTLCSA"
     gene            14863..16206
                     /locus_tag="PKN53_06550"
     CDS             14863..16206
                     /locus_tag="PKN53_06550"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD88644.1"
                     /translation="MKKLAFILVLLSIGVYGFAQQENISEDIETLKEMVKQGSHEAMG
                     KLGYCYLTGTNVEKNDKEAFKLLNNASKFGDAFSNFYVGKCHYEGIGTPKKYSKAFDH
                     MSVAGQQNYEQAYKYLGRMYYFGEGVTKNKKEAVKWLKKAIEDEKDIESLYLLATCYD
                     TGEGILKNEAKSLEYFEKAAEKGHVQARIAQANAYKVGKGVAQNTAKAVELYTLIANE
                     GDKDAAYFLATCYEKGDGVGQSYNSAFQWYLKAAEKGNIEAQNKLGECYYKGIGTTQN
                     YDEAFSWFSKAADAANLEAYNNLGNCYYNGHGTTRDYKKAFANYVKASKDEMPEALYN
                     QAVCYSKGQGVKFNIRTSAQLLFKAAQAEYAPAQNLLATYYINGTGVDRDYKKAIIWL
                     EFATNQGNADAFYNLGNCYAKGLGVQKDKAKASSLYSRAAELGHEEAKKELKKVQ"
     gene            complement(16239..>18604)
                     /locus_tag="PKN53_06555"
     CDS             complement(16239..>18604)
                     /locus_tag="PKN53_06555"
                     /inference="COORDINATES: protein motif:HMM:NF037085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HOD88645.1"
                     /translation="KRPITVTVVPGQSKVYKAAEPASFTYTYSGGLIGGDNFTGALSR
                     EPGETAGTYDILQNNLALTNNYQITFISNVFTINPRHITITANSGQNKTYGQADPTSF
                     TYTLSEVLYSGNSVSGALDRVEGEDAGEYEITVGTLAITPNYTFTYQSANFEILPKDI
                     TVIAHQNQSKLYGDPDPVLTYQLIGSLITGDTISGALSREDGEALGNYPITIGTLSAG
                     DNYTILFTSKNFEIKKKNVLVTPNSNQSKQYGFPDPELEFTCSPAIAPWDNFEGELTR
                     EEGELVDTYTITKGTLSLNSNYTLFFTPGVTFEITPRTINVTINPNQSKTYGESDPQQ
                     LTYSYTPDLVGYDSFQGYLYRATGENVGEYQIYHDNLQLTYNYILNVVETNKFNILPR
                     EITVNANAASKTYGNNDPFEFTYSTNGSLAFSDYFTGELEREQGEDVGIYEILGSNFS
                     INNISNYNLIYNSANFTILPRQITVKATEGLSKTFGENDPEFTYSVTSGTIVNNDEFS
                     GELTREQGEDVGFYEIQQGELSLGTNYNINYQPAQFEILPIMLTVIADQGLTKIYGED
                     DPEFTYTVQGNTEPVNFSGELNRTQGENVGTYEINEGNLQAPTNFDFNFVPANFEILK
                     ATPEIYWENPADIYTNQALSEVQLNASANTEGNFTYTPDFGTYLPEGNNQELHVLFNP
                     TDNQNYNQAEKTVHINVLLGTNLAENIKSQLSVYPNPAKDYINLHLNGAIADKYEVYD
                     ITGKLLTTDIINSDTTEIFIDKLNPGVYLLKVYNNNQNIAILRIVKN"
BASE COUNT         6431 a         2728 c         2891 g         6554 t
ORIGIN      
        1 aataacaaca gaacaaggaa ttttttacag tgcagaaata gagcttaatg attctattct
       61 taaaactaat tacaataaaa ttatcccatt ttcccaaaac atgacaggaa cagcagaaat
      121 aattaccgac gatcttcgct tatcagaaag atttttaaac ccattgcggt cgatttggaa
      181 gaaaagtgtg gagtgaaata tttgaaaatt taatccattt actccaccaa tatccaacgc
      241 cattgtttgt ttgcccaaaa ttcaccagca taatcaattc caattcttgt tgatgtttta
      301 agttttattt tttttgaatc atcttcaacc cataagtttg gacaagttgc aatgttttgt
      361 ttgttaaagt ttttatcaat tttaagtgct tttgttaatt ttcctggacc attataatcg
      421 ccagcagctc ttattaaaac agcttgagga acatcttctt tggaagtaac aaaattcaac
      481 atataataca ttccataaat taaataaatg tatattgtac cagcctctag gtacattgtt
      541 tctgtgcgag gagtttttcc ttttgaagca tgacaagcct tatcttcttc tcctttgtat
      601 gcttcagttt cttttatttt tagtcttaat tcactgccgt catcaaattt gcgaacaaga
      661 attttaccta ttaaatcagg agctacttct aatacatcac gattaaaaaa ttctgcttta
      721 agaatattag ccattatgat ttttgtaaaa agcttttaat attctgtttt tgtttgtgaa
      781 ttttcccatt ttctaaaagc ttcaagagct tcttctctca taaaattttc ggtttttaag
      841 tctctttcgc ttactttttt ctcaaattca tcgtaaataa acttatcgct aaatccaatt
      901 ttatcggcat ctacttttgt attggcaaaa tataatttgt caattctagc ccagtatatt
      961 gcacctaaac acattggaca aggttcgcaa gatgcataaa tttcgcaacc acttaggtcg
     1021 aaattaccca aaatctttgc agcttttctt attgcattta cttctgcatg tgcagtaggg
     1081 tctaaatcat ttgtaacact atttccagat gcagcaataa ctttattatc tttaacaatc
     1141 acagcaccaa aaggtccgcc acctttatct atattttcaa ttgataaatc aattgctaat
     1201 ttcataaaat cttttttggt catattttta gtttttttct ttaacttgtt ctatacccca
     1261 taaaaataaa gagtagaaaa atgaaataaa tactacagcc gaagatgtct ccaacatgtc
     1321 gtctgcaaac atagaaaagc tcgcaattag gaaaaataat ataaaatacg gagatcttga
     1381 tctaaaattg gtaaaaattg gataaaacca cgccaacata caaaaactag ctccaataat
     1441 accaaaagtt atgtaaaaag taagaaattg gttatgtgct ctatgtctgt tttgtatgtt
     1501 tattttagaa tcaaattctt cataaattgc tttatattca tcatctaaat cgccagtgcc
     1561 agtgcctaat aataaatttc gcttggctaa gattaaacca tacttttgaa attcaaatct
     1621 ttgagttatg gaatgtccat caggaggaac accttttaaa taaacatcaa tctgccaaat
     1681 tatattataa actctttgac ttattcctct tttatcaaca aaacgatagt tggtatgtcc
     1741 tgcttcaata tttacaatgt ctttatccgt tagctcttta aatccttcag catctttgtc
     1801 caaacctttt gaagccatat atcttactat agtatgcaat aataactgtc ctttagcgtc
     1861 ataaccatca aaattcatta aacttctatt gttccaagct actcttaatt ccttttcgca
     1921 aacattatag taaataaaat ttccattttc taataaataa actgtggtgt cagatacata
     1981 aggattaccg taaatagaac attcttttaa ttgaatatca gggttggaag gcgtgtaaaa
     2041 atctttaatt tgaacactta gatatatagc tatactgcct attaaaagaa taagaacaga
     2101 gaaactcaaa attttaattt tcttgttttt gtgagtaaac attttgtaag ctaaaacaca
     2161 tatactcgtt acaattaaaa ctccaagtcc tgtaaaacct tgaataagag caaaaaagag
     2221 cattagccaa aaaattattg ctattgcaat tagataataa attaattttg agaatttcct
     2281 ttttataata aaatatgtca ataataaaat tgcgaggctc atcataacag ataatgttat
     2341 atggcttata aacaccgaat agtgtctaac attatcagat ttcagttcga aataaattcc
     2401 caaatatgcc aataaacctg ctaatgtgct agttaacact ccagctataa ataaaaaaac
     2461 aatggtttta aattgtttat ttgttagtgc ttttgaactt gctattatta ttggtagagc
     2521 taaaagtgga agtctaattt taatgtcgcg gagagcaaac tccatgtttt gagaattgaa
     2581 aaaccaaatt aaaggaataa aaaataaaag taaaaaaaat agtagttctt tattttttga
     2641 aatagttttt atcttttgtt taaaatttgc ttcaattaaa taattagttg aaagcattat
     2701 aatacaagca ttggtaagaa attcggaaat aggcataaag aaagcaataa cgcctaatcc
     2761 gaatatatat atatatctat gaattttctc tctcactagc cctaaaactt agttttacat
     2821 ttccatagaa gcgctagatc taattttctt atagccaaat gtcatattac aacccattct
     2881 aatattgaaa tttttagctt tttgaggaaa catcatgttg gtaaaactgt ctgttactat
     2941 ataccaatta aagatagcag ctcttacatt tagtccgaat ccaatattat ttatagaatt
     3001 gttggcataa gaccaagtta aatatgcagc taaccagttg gttatattag aactagctcc
     3061 aatagtaagt gattgtaaat atgattttct gtaaaactct cctctataaa gtacattaaa
     3121 gtgtagtttc tcatggaact tatattttcc accaagatat agattagaag gcaaccaagt
     3181 tatatagctt gtttcaagta aatcaaattt aaaagtatta attaatgtgt ccataagatt
     3241 atcaacagat tcatcaaatt tattttcatt cttgtcaaaa atcttaaatt ctatacctct
     3301 aaaatcgtat tctccttcgc tcataaaatt atgtggattg ttgctccagt ttatgaaacc
     3361 aaaatcattt aatgaaccgt aaacttctat ttctggattt attttgtaat ttgctcctaa
     3421 gtcaaagcct ataccaatat ttttcatatt taacaaaaat gctgaagcca tcgcacctgc
     3481 accttgattt tgtagtttat caaattcttc ttcgtcaata cccaaatgaa ttgagtcagg
     3541 tgtcattata tattcatagt ttaaaggcga tgaagtttgg attttcatat ctgttaccca
     3601 ggtcatcata taattttctg gatcagtatg aatagatagt tttttaatat ctgtattaaa
     3661 attagattga ccaaataaaa ttttcatgcg tcctcccaat cttaattcct catttgcctt
     3721 catcgacatt cctacagcaa attctttata atgtgtaaaa tttaaaccaa atcctttaaa
     3781 atcatgtggt ttaccttctt tcatgtatgg aatgttacca ttaatcgcaa actcaaataa
     3841 atcgtaaggt agcgaaaatc catatttaag tttttctgtt agagaaatag ttaaatacat
     3901 attttgtaaa cttataccta agtttaaaag ttcaaattga gtatcaaatc taaaatgagt
     3961 tgttttattt aactttttca tcattagtgg aaggtctaaa accaaactgt cggctttgct
     4021 accttctcct ttgtgaaaaa tatgattata ataaatcgag ttgttggcat aattgaaata
     4081 cattgctggt ggcatttctc caaataaagg aagtataagt ccgcccattt gaaatttata
     4141 gtctatctca gctgcagggt ttacaaattg tgattgcata actctgtcca tattataaaa
     4201 agtgagagcc tgttgagcgt ttacagaact aaatagacta acaaatgcta atatgattaa
     4261 atgttttttc attttcattc ttttttaaat ttaaacttat ttaccaatag agtctatagg
     4321 tccatcgtat ttaacatcaa cctcaaatcc tatatcaaat ttgatttggt aatcggtata
     4381 tatttttaca actttatcgt ttgcagcatc agtggtataa ccatgaacac gaagtattat
     4441 atattttcct cctctaactt tttctaatct atcgaggtcg tattctattt ttgttatttt
     4501 ttgagtaaaa tctgtaactt ttccatttgc atcaactttg gcaggaccaa taagttgctc
     4561 aggacctgct gtgaacactg aatctaaaat attataatat ttgtctgtaa tataaatttg
     4621 acttatagct tctacaggga atccattatt aaattctgtt cttaaaagag ctcttgttat
     4681 agggttgtct tcactaaaca agtctgttaa atcaatatct aaagtgtctc tgaggtcaaa
     4741 attataaata tatccccaaa gtgggaattc cataactact tcaacatcaa tttttgaatt
     4801 gtcggcaaca aagttattat gactatttcc tgcaggatta gtagtagctt tagctttaaa
     4861 ctgtatccac ttaggtcttt tgttaacaat atcgtttatg tttgaattgt tgcggcttag
     4921 ttttaaagaa tcaatcataa catctccata attggtagaa taagaaatat cataagggtt
     4981 tgattctcca ataggaagtc caacgccata gtcgataata ctatattcag ttccgtcaac
     5041 agccgaagcc gccatcatgt gggtaaaata aaattgtgaa ggtacaccat aagaatttgt
     5101 gtagaaaact tctaattttg gatcttcaaa tctatatctc tcaattgtcc aatcgtcagt
     5161 gttaaataac gatatgttaa tggtatcact ttcaaaaaat aaagtattta atccaaaata
     5221 accatgcata gttttatacg tcatgtcttt catgtctatt gaaaagtcta tgcttcctgt
     5281 gtttggaacg ccatttcccg aaaagaataa ggttagttta agttctatag gaatttcatt
     5341 aaaattagca gcagtttgag ttaaatcaat tttatatccg gcaaagtcat ttcctaagct
     5401 atgcacagat ccacctcctg gaacataagt aaatgttcta gtaaaagcct gtccgttttt
     5461 tgttatactt gggaaagtaa catataattt tgcagaatgc ccataagtag atgtggttct
     5521 aatgtccgca ataccagatt ttaataaaat agaatctatt tctgcttctg gattaaacat
     5581 agtaaatggc acattttgac taatcgtatg actaatggtg tctccaaatt ggtcaaaccc
     5641 actaaaatta aatattacac taggtatgct tccactagct gtttggtcgt ttaagtaaat
     5701 aatttcttgc acagcagctg agctaactct agttttatac tgcattgaaa cataaccttc
     5761 gtcgtttttt attaataagc ccgttgagtc aaatctttct aaggcatcag taaaacttag
     5821 gttaccccaa acagcaggag ctgcaaatcc tgcctcataa ttaatttctt tatcccattt
     5881 gtcaaaatca aaattatctt ttatacacga agaaaaagat aaaaatagaa taaagaatat
     5941 gagcaaataa caaattctag ttttcatatt tttagcgttt aattaatttg taattataca
     6001 ataaattttc caaaccacca acagttaaag acattttttc atcattagca ttaaaaaaac
     6061 ctatactaaa aggacttttt cctatcaaag gaaacccctc aaaaatgctg ccattctcgt
     6121 ttattaaata aatcttttca ctttctctac aaactattcc aatttttatt tgattattag
     6181 aaaatttata aatactaggt tcgaaagata ttttatcatt aaaatttaac tcaaataaaa
     6241 gttttttatt gttattatat acttctaatt tagtgctatc tgcaaaaata tagtcagaat
     6301 ttccatcata atctatgtct gctactacaa aatagtggtt ttcactaaat tcttttataa
     6361 atagtgaatc ttgttgccct tgtggtgaaa aatatctaac attgccttta gcatcggtgc
     6421 atacgaactg agatttgcct ttgtttttat ccaaataaac tttgttgttt tttgaaaatt
     6481 taaaatttgt aaaatatgta tttcttggtt cgcctcgtct gtttaaacta tagaatttaa
     6541 aatcatcatg aaatactaag taatctttat tgtcgatagt ataatgtgat atttcagaac
     6601 taacaaggtt ttcgctatta ttaaaattcc agcctttgac aatttttcca gtctcgtcgt
     6661 ataaatatat ttttttatcc tcacaagcaa tagctattct atagtttcga ttgttttcat
     6721 agtcgaaaac cgaaagagga gaactggctt ttgacctaag attaatagga aaattcttaa
     6781 cattattgcc cagcctgtct attaggtaaa tctttgattt tgtagaaaat aaatattgga
     6841 gtttcccatt tttaaaagca tctatttgat aaacgttacc aaaaatactt tcgtcaagtt
     6901 gtattttcca aatttctaaa ccactgcagt taatcaaata taaattattg ttttcatctt
     6961 ggactaaaat ttctgtacta ttatcatcat ggttagttac aataaaaggt ttggtgctta
     7021 caggagcatc aattctgctt tcccacgaag tttttggttt ttctgaaata ttgtcggcat
     7081 attgtaaaat catattgttg tatagcctgt cgctagcatt gttaatttgg taagcccaaa
     7141 aactaaactt ttgtagctca gtaaatttac tttgagctaa gttttgcaaa tctgagttta
     7201 agtaatcttt gattagagga aagccatttg ttaatgaaaa atatgtaaag agaagcgatt
     7261 tgtttgaaaa gttttctaaa aacttgttgt gttctatatt ggttgtcatt gttttattta
     7321 aaaatgcagt ttttgcaaaa tttctcaaac cttctttggt ttcagcaaaa accataaagt
     7381 ttttaataaa acaaacataa ctagaattgg attttgtaaa aagtggacca aaaagtatta
     7441 aaggtaaatt gtcagcagga agtttatagc atgctaagtt tgtatgctca tcaactttta
     7501 ggtctgattt aaaatccttt atatttttat tgagtttgct agcataattt tgaataatgt
     7561 tctccagctc aaatatgcca acagattgtg agttcaaatt taaaatagta taaaaatcaa
     7621 gtttgttgtt ttctactata gccgaaatac aaatttcatt ttcaattaat ttgtaaaaat
     7681 ctttaaattc aatgttgtag gttgttttaa gttcattaat agcttcttct cttttatcaa
     7741 gttcgccaat tgattttaaa taactgttta aactattgtt ataattttca gaatcagtaa
     7801 atgctaaaat tgtataataa cacgcattgt ctggtaaaat gtctaaacag ctttgataac
     7861 aaggcgattg ttgctttaat atatttgata atgctggtaa gctatcttta ttttcgataa
     7921 aaccattcat gctaagtttg cctgtattta tgtttaaatc catttctatc cagttggcaa
     7981 actcatgttt tgaatcaaaa tcttcggcta agttttgttt taaaatcgat tttagttgtg
     8041 gtaaatttat ataaacattg gctagttctt ttttgcctgc agattcatac aatgctgcaa
     8101 attcactatc tcttttgata atagaattgt tgctagctaa agcatcaata gacttttcga
     8161 ctactacagc agaattggaa attataagaa tgtgattgct aatcgaataa aaaaactttt
     8221 tgccattttg taaactacaa tcaaagattt tattgccgtt ataatctctt tcgttaatat
     8281 ttgaaaattg tttaagttcg ttgtttaggt ttgtttgaaa ttgtttggtt tttgcttttt
     8341 catcaatctc aatatataaa gtttgagata agctacttaa tgaattgtca attaaaaaca
     8401 aactttttct atttttaact tctttaaaaa aatctgattt actaatgcta tcaaaacaat
     8461 gggcaagaga gtttgatgaa ttgacttgaa aaaacttttg aatttcatat gagattacat
     8521 aatccaaaga taatttttca aaaaatgaat taaaactatt tgtttctatt attaaggctg
     8581 gattgccagg aacggcatca agtgcttttg aagttttttc tgaagatttt aagaaaaata
     8641 atactgcaaa cactaaaagt ccgcataaaa caacaatgat agaaattatt aaaactcttt
     8701 ttttcatagt aaaactaatt actcggtaaa aataatataa tgccaccatt aaaacaacct
     8761 cataaatgaa aacaaattaa catactgagg ttaaaaaaac tattatttca aattgtttta
     8821 ttaaattttt tgtgtgatat tagcaaaaaa aatattattc gtaatatagt ttaaaatttt
     8881 atggcaaaaa ttatcatttt tgttagttta tttattttta gcaaggcttt attttgcaat
     8941 atagacggtg atggagcaag tcgtgtgagt gcttgtgaat cagttagtta tattgataga
     9001 gtaaatagtt ccttatcatc tttttatgat tcaacaaaca tttggtgggc tagtcaatct
     9061 gttatttctg atagtttgcg aaaagtaaat tgcccatatt atccagaata tgtttacaat
     9121 agtagaattg cagatttaga tgaaaaaacg ccaattaagt tagagtataa cgaacatgtt
     9181 ttgaaatata ttgaggctta tggtgttaaa aatcgtccca aattacaatc tataatttct
     9241 aaatcggcat attattttcc aattttcgaa gaatatttag ataaatatgg tttaccttta
     9301 gagttaaaat atcttgcagc tgtagaatcg gcacttgatg ctagagcagt ttcaaaatcg
     9361 ggagcagtag gtttatggca gtttatgaaa ccaacatcag atttattgga cgttaaagtt
     9421 acttcatata tgatgagcgt agagatgtat ataaatcaac tgatgcagcg tgtaggtatc
     9481 ttaaatattt atacgaaact tttggagatt ggcaacttgc tcttgctgct tataatggtg
     9541 gtcctggaac tgtgaaaaaa gcaatagcac aaagtggtgg aaaaacaaat tattgggatt
     9601 tgcgtcctta ttttaccgag caaatgagaa attatgtgcc tgcgtttatt gcaatgaact
     9661 atttaatgga atatcatgcc gaacacaata tttttccaaa cgatgttttt atcgaaagtg
     9721 taaaaactga tactttgtat gttaacggat atgtacattt aaaaaatatt gcagaacttg
     9781 ttggaacaga ctatgaattg ctcaaaaatt taaatccagt ttatacttat gcttatattc
     9841 caatggatgg agacaagcat tgtttggttt taccttttga tgttgttcat aaatattatc
     9901 aaaaagagca agaaattgtt gctttgaaaa ataatgttaa aaacgacact gtattaactc
     9961 aaccaccgtt tatgcaaaaa cctgtacgcg aaaatattat ctatcataat gtagaacgtg
    10021 gagataattt atttagaata tctcaaagat atgcttgtac tattcctgaa atctatcagt
    10081 ggaataatct agacgacaat tatatcttgc gagttggaga cagtttgaaa attatgatag
    10141 aataaaatta tatattataa atctttgaaa caaatagttt tgaagaaaaa gatatttata
    10201 ttatatttat aaaaaaaaaa ttttgcatat gtttaaattt ttacgaaaag aaaacacaat
    10261 agccggtcct aaatttaatc gttggttagt tccgccagcg gctttggcta ttcatttgag
    10321 cattggaatg gcatatggat tctctgtttt ttggctacca ttatcaaaat ctataggcat
    10381 aaccgaagca gttttagctc ctgaagggat tagttttatt gaaagagttt tttctactgc
    10441 ttatgattgg cccatctcaa tgcttgggtg gttatatact ttgttttttg tgtttttggg
    10501 tttgtcggca gccatgtggg gcggttggtt agaaaaagta ggtcctcgta aagtaggatt
    10561 tatttctgca atattatggt cgggcggact gctaatatca gctcttggag ttgtcttaca
    10621 ccaaatttgg atgctatggc taggctcagg agttattggt ggaataggat tgggcttagg
    10681 atatatttcg ccagtttcta ctttagtaaa atggtttccc gacaaaagag gaatggctac
    10741 tggaatggca ataatgggat ttggaggagg agcaatgata ggagctcctt tagcacaaaa
    10801 acttatgaac ttctacgcca ctgatactag tgtaggagta tgggaaacat ttgtaacctt
    10861 agcaataatt tattttattt atatgataat tggagctttg tcttatagaa tacctccaac
    10921 aggttggcaa ccaaaaggat ggaaacctaa agaaaaaaag cgaaaatctc tgattacaca
    10981 acatcatgtg catgtaaata aagcttggaa aactcctcag ttttggatgt tgtggctagt
    11041 gctatgttta aatgtttcgg caggtattgg agtaattgga atggcttcac cactactgca
    11101 agaagtcttt ggaggaaagt taataggaat agataaagtg ttttcagaat taaatgaagc
    11161 agaattagca aaaattgcat tggtagcagc tggttttact gggttattat ctttatttaa
    11221 tattggagga cgtttcgtct gggcatcact ttcagatgta ataggaagaa aaactacttt
    11281 cgcaatattt tttattctcg gatttatttt atatgtctta gcacctcacc tcggactagc
    11341 tggtactgta gctcttttcg tagtcgcatt ttgtatcata atttcaatgt atggaggagg
    11401 cttcgcaaca attcctgcat atcttgcaga tttgtttggc acacaaatgg ttggagcaat
    11461 tcacggaaga cttttaactg cttggtcaac tgctggtatc ttaggacctg ttttggttaa
    11521 ttacattaga gaattccaat taaatagagg agtgcctgtt caacaagttt ataataccac
    11581 tttttatatt ttagcaggac ttttgataat aggcatgata gcaaacttcc tcgttagacc
    11641 agtaaataaa aaataccata tgagcgaaga agaactagca aaacttagac ttgcaacaac
    11701 taaagaagaa aaaaacggaa acactaatgt ggacttgagt gaagtgggtt cagcaaaagc
    11761 tacatggaaa cttatatttg cctggacagc agtaggaatc cctttgctat acggaatata
    11821 ttcaactgtg cttgatgctc tacctttatt tttgagatag tgatttttgt taatactatg
    11881 tttgaaaact ctttgcttta tgttttagat tattaaaatc attattgtat ttttgtgaaa
    11941 aaataaattt aatattatgg caaatagaag agaattaaaa aaagacatta attggcttac
    12001 cgaagaagtc gtttctgatt gtttaattta ttgggaattt aacaaaaaca aaaacaaaga
    12061 acatgtagta gatataatta attctgtaat aactaaaaga aatgaactta tatcaagaat
    12121 taataaacct acttcaaaaa tgactcgcca cgaagttaag tctgcttata gtcaagtagt
    12181 taaggagatg tttgaagtaa caaatgaaag tttagaaaaa ctaagtgaat tagctaagaa
    12241 aaatgcaaag gctaattaat attcccgaaa ttggaaatat tgttgtaaca aaaaaaacta
    12301 atgcaaaaaa ctatagatta agagtacatc ctcaaaaggg tgtacttctt actattccta
    12361 gatttggtaa ttttaaagaa ggagaaaact ttgtcttaaa aaatatagat tggataaagt
    12421 caaaactaaa agaaagagaa attcgtcaac aaaacttaaa aatatttaaa cctggagaag
    12481 aatttatcac acgtaattta agtttagaat tttttgaagg aaactttaaa aatatgcttt
    12541 cggcatattt tgaaaataaa aaaataaaaa ttttatacca aactggattt aacgattttg
    12601 aagcagatga tgttcaagag tttattaaaa aattcatatt aaaatgcttg cagcaagaag
    12661 caaatattta tttaattcca agattaaacg aattgtctca aaaaacagga ttaaaattta
    12721 aaaattgtaa aataggaaca gcaagcacac gattaggttc ttgtgattca aaaaacaata
    12781 ttattctgtc agcatattta atgcttttgc ccgacagact tatagattat attattttac
    12841 acgaactttg ccatatttta cataaaaatc atggagaaaa attccacaat cttttaaatt
    12901 tattgacaaa tggaaaatct aaagaattaa ataaagaact aaaatcgttt agaacctcta
    12961 tagaacccgg aaattacaag ttttaattta ttttgctctt tctacgtact ctttggttct
    13021 tgtgtcaact ttaatttttt cgccaatgtt tataaaaaga ggaaccataa ttgatgcgcc
    13081 agtatcaata gttgcaggtt ttaaagcatt ggttgtagta tcgcctttta atcctggttc
    13141 agtataagta acttcgctaa taataaaagc tggcaattcg caagttaaag cagcctcatt
    13201 ctcagcgtgg aaaataactt caacttcttg tccttctttc aaaaattcaa taccttcaat
    13261 caagtcttcg gataaacaaa cttgttcaaa agtttctaag tgcataaaat ggtaacctaa
    13321 atcatcatta tataaaaatt gataaggacg gcgttcaatt ctaattatat caatttttgc
    13381 acctgaagga aaagtgtttt ctattactct gccgttttct aaacttttca ttttagtacg
    13441 tacaaaagct gctcctttgc caggtttaac gtgttgaaaa tatgtaattt gataaggttt
    13501 gccattaaac tcaatggtta atccattttt aatatctgct gttgttgcca taattttatt
    13561 tttttttaca aaagtaataa tatcatttgc tttacaaaat ttttttttaa ataaattgat
    13621 tttttagccc ataacatcac atttagcaag gctagataaa aaatatttat taaaattaga
    13681 aaatttaagc aaaaatattt atattcgtgg aaaattaaaa cactattgat atgaagaaaa
    13741 ttttaatgtt aacttttatt gcttttattg catttgcttg taaaaattca gctcaacccg
    13801 aaacacaaga acaacaaaaa aaagaagaaa taaaatttga gtgggcaagt tatgaaaact
    13861 ctaggttcga cttttcagta tcttatcccg attttctaat tccgcaagga gaatctggaa
    13921 atggagatgg acaaagattt atttcagaag atgaaaaatt tcaattttgg gcttatgttg
    13981 attttaaaat gaatgcaact tttgacgatg ctccaagttt tgatgaggct tacgagcaag
    14041 acgtaaatca agaaaatgtt gagtacaatg aaaaaagaca agataattat tatgtgagtg
    14101 gacatattga taaaaatact cttttttcaa aatacacata tttccgcaat atgaattatt
    14161 atgtattgta tttccaattt ccagatgcag aggcaaggtt cttttctcca attgtagata
    14221 caatagtcgc ttcgtttgat tgtgatggag gtgaaaatca tgatgtcgaa aattttatta
    14281 atggattttt aaaagattgt tggataggga aaaattttga cgctttactt agagataatt
    14341 cagcaatttt aaacaaatat atagacaaag atttaggaat agctcgactt catgcgcccg
    14401 gaacaattcc aagatttgca aaaagaaacg aaaattttgg ttatgataac tataccgact
    14461 ttgaaactac atttaaaggt aaatatactc ttactatgtg caaagaaaat tacgatttgc
    14521 catgtaattt tgactttgaa gacaaaaatg gaaaaattgt atgttatgat tttgtaagtg
    14581 atttaccaaa tcttgttgac ccagaaactt tcgaaatatc atcacttacg ccaatgtatc
    14641 caagctcaaa aattatgctt gtttatttac caaataaata taatagtccg cgtgcatttt
    14701 attttatttt agtaaacaaa aattggaaac taataattgt tgacgacaca ctttgttcgg
    14761 cataaatctt gaaatatatt tataaaaata tatagttaaa aaaaataatg ttttgaatta
    14821 aattattttt aatatatttg ttgaaatttt aagtccttag atatgaaaaa actagcattt
    14881 attttagtct tgttaagtat tggtgtatat ggttttgctc aacaagaaaa tatctctgaa
    14941 gatattgaga ctttgaaaga aatggttaaa caaggaagcc atgaggcaat gggaaaatta
    15001 ggatattgtt atcttactgg aaccaatgtt gaaaaaaacg acaaagaagc tttcaaatta
    15061 ctgaataatg cttcaaaatt tggcgatgct ttctcaaatt tttatgtggg aaaatgtcat
    15121 tacgaaggta ttggtactcc caaaaaatat tctaaagctt ttgaccatat gtcagttgct
    15181 ggtcaacaaa attatgagca agcatataaa tatcttggca gaatgtatta ctttggagaa
    15241 ggcgtaacaa aaaataaaaa agaagctgta aaatggttga aaaaagctat agaagatgaa
    15301 aaagatattg aatcattata tttactagca acttgttatg acactggcga aggtattttg
    15361 aaaaacgaag ccaaatcact tgaatatttt gaaaaagcag ccgaaaaagg acatgtacaa
    15421 gctagaatag cacaagcaaa tgcttataaa gttggaaaag gagttgcaca aaatacggca
    15481 aaagcagtag aattatacac tcttattgct aacgaaggcg acaaagatgc tgcatatttt
    15541 ttagcaactt gttatgaaaa aggcgatggt gttgggcaaa gttataattc tgcatttcag
    15601 tggtatttaa aagcagcaga aaaaggaaat attgaagctc aaaataaact tggagaatgt
    15661 tactataaag gcataggtac aactcaaaat tatgatgagg ctttttcttg gttctcaaaa
    15721 gctgccgatg cagcaaactt agaagcatac aataatttag gtaattgcta ctataacgga
    15781 catggaacaa ctcgcgatta taaaaaggct ttcgcaaatt atgtaaaagc ttcaaaagat
    15841 gaaatgccag aagcattata taatcaagca gtttgctatt caaaaggaca aggtgttaaa
    15901 tttaatatta gaacatcagc acaattattg tttaaagctg ctcaagcaga atatgctcct
    15961 gctcaaaact tgttggcaac atactacatc aatggaacag gtgtggatag agattataaa
    16021 aaagcaatta tttggcttga gtttgccacc aatcaaggaa atgcagatgc tttctacaat
    16081 ttaggaaatt gctacgcaaa aggactaggc gttcaaaaag ataaagcaaa agcaagtagc
    16141 ttatattcaa gagctgcaga attaggacac gaagaagcta aaaaagaatt gaaaaaagtt
    16201 caataaactt tacgaaaaat aaaacttata gaatattctt aatttttcac aattcttaaa
    16261 attgcaatat tttgattgtt attgtaaact tttaacaaat aaactccagg atttagtttg
    16321 tctataaaaa tttcagtagt atctgagttg attatatctg ttgtaagtaa tttgcctgta
    16381 atgtcgtaaa cttcatactt atctgcgata gcgccattga gatgcaagtt tatataatct
    16441 tttgctggat ttggataaac agaaagttga gattttatgt tttcagctaa attagttcca
    16501 agtaaaacgt taatatgaac tgttttttcg gcttgattat aattttgatt atctgttgga
    16561 ttaaataaaa catgcaattc ttgattgttg ccttctggta gatatgtccc aaaatcagga
    16621 gtgtaagtga aattaccttc agtattggca ctagcgttta gctgaacttc gctaagagct
    16681 tggttggtgt aaatgtctgc cggattttcc caataaattt cgggtgtagc ttttaaaatt
    16741 tcaaaatttg ctggaacaaa gttgaaatca aaatttgttg gagcttgtag attgccttca
    16801 tttatttcat atgtaccaac attttcgcct tgagttctat taagttcgcc tgagaaattt
    16861 acaggctctg tatttccttg aacagtataa gtaaattctg ggtcatcttc tccataaatt
    16921 tttgttaatc cttggtcggc aattacagtt agcataatag gtaaaatctc aaattgtgca
    16981 ggctgatagt ttatattata gtttgttcct aaactaagtt cgccttgttg aatttcgtaa
    17041 aatccaacat cttcgccttg ctcgcgtgta agttctccgg agaactcgtc attgtttaca
    17101 atagtgccag aagttacaga ataggtaaat tcagggtcgt tttctccaaa agttttagat
    17161 aagccttctg ttgctttaac tgtaatttgg cgtggtaaaa ttgtaaagtt agcagaattg
    17221 taaattaaat tataatttga aatattatta atgctaaaat tgcttcctaa aatttcatat
    17281 attccaacat cttcgccttg ttcacgttct aattctccag taaaataatc gctaaaagct
    17341 aagctaccat ttgtagaata agtaaattca aaaggatcat tgtttccgta agttttactt
    17401 gctgcattag cgttaactgt aatttcgcga ggtaaaatat tgaatttgtt agtttcaaca
    17461 acatttaaaa tatagttata tgttaattgt aggttatcat gataaatttg atattcccca
    17521 acattttctc cagtagctct gtataaatat ccttggaaag aatcataacc aactaaatca
    17581 ggtgtgtaag aatatgttaa ttgttgtgga tctgattctc cataagtttt actttgattt
    17641 ggatttatag ttacgttaat tgttcgtgga gtaatttcaa atgtaactcc aggtgtaaaa
    17701 aacaaagtat aatttgaatt aagagataaa gttccttttg tgatagtgta agtatccaca
    17761 agttctcctt cttctcgagt taattctcct tcaaaattat cccaaggagc aattgctgga
    17821 ctacaagtaa attctaattc tggatcagga aatccatatt gttttgattg atttgaattt
    17881 ggtgttacta aaacattttt ctttttaatt tcaaagtttt ttgatgtaaa taggatagta
    17941 taattatctc ctgcacttaa agttcctata gtaataggat aatttcctag agcttcgcca
    18001 tcttcacgag ataaagctcc cgaaatagtg tctccagtaa ttaaacttcc tattaattga
    18061 taagttaaga cagggtcagg gtcgccataa agttttgatt gattttgatg agcaataact
    18121 gttatgtctt ttggtaaaat ttcaaaatta gcagattgat aagtaaatgt atagtttggc
    18181 gtaatagcta gtgttcctac agtaatttca tattcaccag catcttcgcc ttcaactcta
    18241 tctaaagctc cactgacgct gtttccagaa tataaaactt cgcttaatgt atatgtaaag
    18301 ctagtagggt ctgcttgtcc gtaagtttta ttttgaccag aattagcggt aatggtaatg
    18361 tgtctaggat tgattgtgaa aacatttgaa ataaatgtta tttgataatt gtttgttaaa
    18421 gcaagattgt tttgtaaaat atcatatgtg ccagcagttt cacctggttc tctagataaa
    18481 gctcctgtaa aattatcacc accaattaaa ccaccgctat atgtgtatgt aaaagatgct
    18541 ggttcggcag ctttataaac ttttgattgt cctggaacaa cagtaacagt aataggacgt
    18601 ttta
//
LOCUS       DAOPNX010000047        18375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_3655_length_18375_cov_6.873035, whole genome shotgun sequence.
ACCESSION   DAOPNX010000047 DAOPNX010000000
VERSION     DAOPNX010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18375
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3655_length_18375_cov_6.873035"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..1364
                     /locus_tag="PKN53_06560"
     CDS             180..1364
                     /locus_tag="PKN53_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88646.1"
                     /translation="MKNKLCIFNPEHDLALACDDENFNPPQSAKKFAQDLSVFPIWFS
                     NDKCFVFDYSSSENWLNELKLKFPQLVNANIISSNSDFSEIQKLEVWGWDKTIYKQLQ
                     GLKVDKSLFPNFEKLEKIRELSHRNLAIKAHNFISQNINSDKVLNVSASECCELLGVQ
                     NFVSENLKVVVKAPWSGSGKGLQWVKNKLSDSHLGWISNILEKQKSVILEKQYSVVQN
                     FAMEFLCAENKAEFCGYSLFETDRGRFQYCELMSDREILHKLCSLGVLEESFLEIQKL
                     LIKFIQKEISPHYSGILGVDMFLYSDNSEILIHPCVEINLRLTMGYVARILFDKFINN
                     TKKGKMFVEHNAQKGFFYEDNLTRKQKNPLIIENNKILSGYLSLNNVLPETSYRIYIE
                     VS"
     gene            1443..2960
                     /gene="purH"
                     /locus_tag="PKN53_06565"
     CDS             1443..2960
                     /gene="purH"
                     /locus_tag="PKN53_06565"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOD88647.1"
                     /translation="MEKKINSALISVYHKDGLEIIVRTLEKHNVKIYSTGGTWEFINN
                     LGIDAIKVEDITGYPSIFDGRVKTLHPKVFGGILFIRDNETHQNEVETYEIPSFDLVI
                     VDLYPFEETVKNSTDEAEIIEKIDIGGISLIRAAAKNFNDVLVVSNRSQYQELYSLLE
                     ENACYTSLENRKKFALESFGISSAYDTAIFNWFDNNSYSEFKVSEKSSTILRYGENPH
                     QKAKYFGDLSAMFEQLHGKEISYNNILDIDAAVNLISEFEETTCAILKHNNACGIASR
                     PTLSDAWDAALEADPVSAFGGIIITNQIVDQLTAEKINKIFFEIIIAPDYTKTALEIL
                     KQKKNRIILIQKYRELKEKTYRSVLNGVLVQDRDAKIETEDDFNFITNLRPNEAQIAD
                     LLFANKVVKNTKSNAIVLVKNQQLLASGTGQTSRVDALKQAITKARSFDFDLQGAVMA
                     SDAFFPFSDSVEIAHEAGINSVIQPGGSIRDDETIEFCNKNNIAMVFTGIRHFKH"
     gene            3132..3596
                     /gene="greA"
                     /locus_tag="PKN53_06570"
     CDS             3132..3596
                     /gene="greA"
                     /locus_tag="PKN53_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOD88648.1"
                     /translation="MSYLTEEGLNKLKDELDRLVNVERGNISRQIAEARDKGDLSENA
                     EYDAAKEAQGLLEMKISKLQEIVRNARVLDPSQISTDAVQILNKVKLKNVKTGSTMEY
                     TIVAETEANLKEKKISVNTPIAKGLLGRKVGDVVEIEVPNGIVTFEILNISI"
     gene            3608..4003
                     /locus_tag="PKN53_06575"
     CDS             3608..4003
                     /locus_tag="PKN53_06575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOD88649.1"
                     /translation="MASIFTRIINGEIPCYKIAEDENFISFLDINPLKKGHALVIPKK
                     EIDYIFDNSDETLSKILVFAKKVAKGIEANVECKRIGLVVVGLEVPHTHIHLVPINQE
                     SDLSFSNPRVKLSQQEFEELAKKISKSIS"
     gene            4434..5858
                     /gene="selA"
                     /locus_tag="PKN53_06580"
     CDS             4434..5858
                     /gene="selA"
                     /locus_tag="PKN53_06580"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOD88650.1"
                     /translation="MSKNSAVYKQLPAVSKLLDSDSLSDKIEEYGRQAVTESIRRILE
                     NYRKILKTQSQEISENQIIQDICDDIEKSNKNSLIKVVNASGVIINTNLGRVPLSEKV
                     FLEARDIACAYNNLEFNLETAKRGKRNTHLKNILCKLSGAEDALVVNNAAAAVLLCLN
                     TFAKRKEVLVSRGELVEIGGSFRIPEIMKAAGCKMVEIGTTNKTKLSDYENAISEKTA
                     IIFKAHQSNFVIKGFTEEVEVSELAKLGKKYNLPVIYDNGSGLLEKSDFKALKNQIDV
                     KSSIKAGVVLVIFSGDKLFGGPQAGIILGKTSFIEKLEKNQLLRALRIDKTTIALLEA
                     SCKLYINEKDLYKHNFIFKTLSQTQVELKSKAEFLQNSLKENKISSKIIPNKAMIGGG
                     TNPESETESFAVKILFNEKNKKQNSEISAIVYKSLMKNHIPIIGILRKGELLFDIITC
                     NENELTTISQALCQAMNNTSKKQK"
     gene            5855..7810
                     /gene="selB"
                     /locus_tag="PKN53_06585"
     CDS             5855..7810
                     /gene="selB"
                     /locus_tag="PKN53_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOD88651.1"
                     /translation="MKHLIIGTAGHIDHGKTSLIQLLSGIDCDTHKQEKERGITISLG
                     FSHVGLPSGQVLGIIDVPGHKDLIDTMISGASSINLVLLVIAADEGIMPQTSEHLNII
                     NALGIKHGIIVLSKIDLVDEELVEIAKSEIKDFVQNSILENSPIIGVSSHTGEGKDNL
                     ILELENFVKSYQETEFKDNFRMYIDRLFVVKGHGSVVTGSVLDGKLESGEEVFLLPSN
                     SKKFRVRAMEKYGKPTEIVQKGDRAAINLIGLKQEDFTRGMLISKNEIVSTKLIDVYI
                     SSFDLVPTLKLWTDIIFISGTYQSMGKMHLIDKEKLFAGDDAIAQIHLEKDFVGEIKD
                     RFIFRNSSDDISLGGGFVIDNQPLHHKRRNLELSQKLRNIATNLIQGNELESNILRII
                     EKEPRPFGIDEIVEKTLSSKEEILSEISNLSQNICVYNNEILIYSRFDKMFEDKIIAY
                     LKDYHSVNYIFDTGMQSEEILGKFNLGKSNLVKDYLKNLLERLKENSLLDFYENTWIL
                     KNHKVNIDQKTQSELDWLESEFLAYKDEKPVLLDIEEKASAKNISTKKYKLYLNYFVS
                     KNILVVFNGEYMHSKIFIEAKHKLLNHLKTKENGIYIPEYKEVITGTKKFRGLISDIL
                     ENQGLIKFIKDSNNESVLLITDRGKMI"
     gene            complement(7827..8258)
                     /locus_tag="PKN53_06590"
     CDS             complement(7827..8258)
                     /locus_tag="PKN53_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88652.1"
                     /translation="MANILKSFFILIVFSLCINTNLKAQATAEDLIDAFFQIFEEDAG
                     AAIDYLFATNNMIDPNQPGIKSIKERLELSRKLLGDYHGYEIINVYQAGESYRKYNYS
                     LKFERQPVKFTVVLYCPKNKWKVQNVNFQDDIDTEFSLRED"
     gene            complement(8339..8926)
                     /locus_tag="PKN53_06595"
     CDS             complement(8339..8926)
                     /locus_tag="PKN53_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD88653.1"
                     /translation="MKLKNFSDQELIERFIKGDKECMETLIHRHKKNIYSYILMNVKN
                     SHIADDLFQETFIKVISSLKQGKYVDKGKFSSWVIRIAHNLTIDHFRREKSLNTFSND
                     DSTVDVFNSEKFSHENIEDQIVGEQIENDIRSLIEFLPEEQKEIIMLRHYGGMSFKEI
                     AEQTDVSINTALGRMRYALINLRKIIEEKNIVLTK"
     gene            complement(9096..9773)
                     /gene="ung"
                     /locus_tag="PKN53_06600"
     CDS             complement(9096..9773)
                     /gene="ung"
                     /locus_tag="PKN53_06600"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOD88654.1"
                     /translation="MKITNVNIEESWYAQIKEEFNKEYFLNLKKFLIEEKNKFTVYPK
                     GSQIFNAFNLTPFKDVKLVILGQDPYHGPGQAHGLCFSVPKGVKPPPSLVNIFKELKQ
                     DINFNIPDHGNLEAWAKQGVFLLNATLTVRANNAGSHQNKGWEIFTDKVIQTLSQEKN
                     NLVFILWGRYAGAKENLIDQNKHLILKAAHPSPLSAYNGFLGCKHFSQANKYLKKHKI
                     EPINWNL"
     gene            complement(9773..10504)
                     /locus_tag="PKN53_06605"
     CDS             complement(9773..10504)
                     /locus_tag="PKN53_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88655.1"
                     /translation="MNRQEKMINKMREIAQLNPEKIHIIETNVGAEMQLEFFETLQSF
                     TKEDNIPKPEQIWENLNKAETSLEEKKKSLALLASFGEVDIFRMIEKYLENPEKELQS
                     WAFLAYQQARMFLEANLLDENKIYIASGLGGKGHRLRYAFCFIAKENFTDTQQNIFKG
                     EIEYSFSKNDSILEELSFTGKYAIGTVLIALHVDIVDLIENIIKEINQYGNFLHENIY
                     ITNEKIIDTNQLDNLFKHIENSDNE"
     gene            complement(10615..11064)
                     /locus_tag="PKN53_06610"
     CDS             complement(10615..11064)
                     /locus_tag="PKN53_06610"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOD88656.1"
                     /translation="MKVENGKLVTIIYDLNVDSYDGELIESIKEDEPAIFLCGAGEML
                     ETFEEKLIGLKEGDSFKFSLTKDQAYGDEDEDAIAEFPIDIFEEDEEGVPEVGDYVPM
                     EDEEGNIFDGIVIEIENDTVVLDFNHPLAGEDLFFVGKVIKIEDTKK"
     gene            complement(11146..11802)
                     /locus_tag="PKN53_06615"
     CDS             complement(11146..11802)
                     /locus_tag="PKN53_06615"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HOD88657.1"
                     /translation="MKTKKTSPNLFFYIGLIAFGFGIATLSIPDFSMKTMVIIIGALI
                     ASGGLGIFIYTLRKKPENSFIKISQIILSLINIAFGIVLIIIPNFFIKALLILLGVLL
                     IMTGVLHLITTLNIKPLNNFGKMFIAIAAIMLIAGAIFIINPFKNIQDITVFFGIVFS
                     VYGLSNIMMSFWLRSEYAKDKKQDKETLEIDAKVIDTEVDETEETKETDETNKTEEAK
                     "
     gene            complement(11834..13048)
                     /locus_tag="PKN53_06620"
     CDS             complement(11834..13048)
                     /locus_tag="PKN53_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOD88658.1"
                     /translation="MKDYKQININNYNYDLPENKIAKYPKANRDESMLLIKNKDSISQ
                     SVFKNITDFLPTNSLMFFNNTKVINARLFFQKETGSIIEIFCLEPIPINYSNALNSTK
                     QCTWKCLVGNNKKWKSGNLKKNININSQNFELKIEKKQQLGNAFEIEFTWDNENIIFY
                     QILENSGNVPIPPYLNRESESIDNQRYQTIYGEKQGSVAAPTAGLHFTETVFQNLEAK
                     NIKTDNITLHVGAGTFQPVKTTTVAEHKMHTEYFIVSKSNIENLLAYLGKTIVVGTTT
                     VRTLESLYHIAVQIYFNKNKKDNYFEISQWEAYEEKKENIPTETLLKNMLNWMNENNF
                     EQINCSTSIMIVPGYKFQFTNQLITNFHQPQSTLLLLLAAFIGESWKEVYEYALTNNF
                     RFLSYGDACLFL"
     gene            13126..14139
                     /locus_tag="PKN53_06625"
     CDS             13126..14139
                     /locus_tag="PKN53_06625"
                     /inference="COORDINATES: protein motif:HMM:NF021179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2027 domain-containing protein"
                     /protein_id="HOD88659.1"
                     /translation="MKINIGDKVRFLNDVGGGIVVKFLKNNMVLVENEDGFEYPINSN
                     EIIVVEKAKPPEPAQTNKVSDKVIEQKNELKVKLKKDKLTNIIFGFVRNAEEQQDGFD
                     CYLINDSNFSLFYHVVLKFDSGLKKIDAEILEPNTKIYVTHLSRDEINSGKELIIQIL
                     FFDNPHQVLRPQIERKIKIVPLNFFQEHLFIENDFLDDKAYIFELLRDDGSSLKSEKT
                     AKDLEKMFLEKESAEEDKSKRYSARQERKTIEVDLHINELVESVVGMTNAEILEQQMK
                     VFHQTMTDAIMSNAGKVIFIHGIGNGTLKATIRESLAKQYKVIFEDASFREYGFGATM
                     VLL"
     gene            14160..15398
                     /locus_tag="PKN53_06630"
     CDS             14160..15398
                     /locus_tag="PKN53_06630"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD88660.1"
                     /translation="MKFGLFVENLKISINSIRSNLLRPVLTVLIIAIGIMALVGILTA
                     VESIKTTISSEFQSMGANVFTIRRQSSERHGGGFRRVERKNISYEDATLFKNQYSFPA
                     RVSIMTNSSGSATVKYGNIKSDPNISVYSVDDEYLFTSGYEIDKGRNFSSQEVLSGEN
                     IVIIGSELAKLLFIDSESGLDKFISIGSLRYRVIGILKEKGTAFGGSGDKFCWIPLQN
                     GRQYFTASQNTYIISVMPHDISNIDLAIGEAEGLFRSVRKLSVYDENNFAIRKSDSLA
                     NMLIENLSVVTIGATIIGLITLLGASIGLMNIMLVSVSERTREIGVRKALGASSTAIK
                     QQFLFESIFIGQIGGILGIILGIIIGNLISLLVGGSFVVPWLWVLIGVVLCFLVGIGS
                     GYFPAVKASRLDPIVALRYE"
     gene            complement(15458..16465)
                     /gene="gap"
                     /locus_tag="PKN53_06635"
     CDS             complement(15458..16465)
                     /gene="gap"
                     /locus_tag="PKN53_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOD88661.1"
                     /translation="MTKIKVAINGFGRIGRNVFKIAFDRDDMEIIGINDLTDTKTLAH
                     LLKYDSTQGIFDRNISYNETSIIVEGKEIKVSAERSPINIPWPTAPDVVVEATGIFRS
                     KESPKGGYGDHIKNGAKKVVLTVPAKDNIDAMVVLGVNDEKLTPEVQCVSNASCTTNC
                     MAPIVKILNNRFGIENGLMTTIHSYTNDQIILDGPHSDLRRARSCAISQIPTTTGAAK
                     AVGKILPELNGKLDGMAMRVPTPTGSSVDLVVNLKTDTTIDEVNQAIKEAAEGPMKGI
                     VQYTEEPIVSVDIIHNTHSSIFDAGCTMLNGRTLKTITWYDNEWGYSCRVVELVAKLF
                     K"
     gene            complement(16562..17377)
                     /locus_tag="PKN53_06640"
     CDS             complement(16562..17377)
                     /locus_tag="PKN53_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88662.1"
                     /translation="MKYLLLFIFGVYLCLGSSFAQGELETSREPDKFFVNSFGIKLNS
                     NGFGGYYSHTTKINRNYSRFFEAEYNYMKSPKELKVINPYFQSLYIKKFVFGKTHSVH
                     NAKFGYGINRVLFEKRDKNSISIFLALSGGVAIAASKPIYYEIVDSVKVVNNKQLYPY
                     TSTHKLNVDIQNNPTDIIGRAPFTLGLKETKFHPGFYLKFGLNFDFSQNIMKSKVLET
                     GFVYENYLIPIEIMSGTKDSNFLNLFICYYFGNKLDAKLNREFRKEQRKLSKN"
     gene            complement(17428..17556)
                     /locus_tag="PKN53_06645"
     CDS             complement(17428..17556)
                     /locus_tag="PKN53_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88663.1"
                     /translation="MKKTILIILAIVLIAIGLSSCKGSEDCPAYGKANIETENTIA"
     gene            complement(17694..>18375)
                     /locus_tag="PKN53_06650"
     CDS             complement(17694..>18375)
                     /locus_tag="PKN53_06650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD88664.1"
                     /translation="SIVLALAMPVSCAEETRTLTVQADSCQYAWNTGETTQSLTIEKS
                     GFYAVTVTDEFGCWAKDSLNIEIYPMPVVDLGDDFEMYENYTAILTAGYGCISYVWSL
                     GPEYTEHFVILSADNLNFGTNVISVTAITVNGCEDSDQVTVTLHQGVNAEIEKAEMFD
                     VYPNPTTGIIKINAINIENISLYDSLGRLVFSTESDTIDMSSFANGNYTLKVLTQDNS
                     YSTKIVKQ"
BASE COUNT         6373 a         2456 c         2922 g         6624 t
ORIGIN      
        1 actgtacaaa attattaaag aaaattttta gggtgtccca atgcggaaaa caggattgag
       61 tttaatagaa cgcgaaattt taaaggaggg taattcctcc tttttttatt cgttattttc
      121 aactttttaa aatattttct aaaagcagta tgaatattta ttatttttga aaatatttta
      181 tgaaaaataa actttgcata ttcaatccag aacacgattt agcattagct tgtgatgatg
      241 aaaattttaa tccaccacaa tccgctaaaa aatttgctca agatttatct gttttcccaa
      301 tttggtttag caatgataag tgttttgttt ttgattattc aagttctgaa aattggttaa
      361 atgaattaaa attaaaattc ccacaattag taaatgcaaa tattataagt tcaaattcag
      421 atttttctga aattcaaaaa cttgaagttt ggggctggga taaaacaatt tataaacaat
      481 tgcaaggttt aaaagtagat aaatctttat tcccaaattt tgaaaaactt gaaaaaatac
      541 gagagctttc tcacagaaat ttagcaataa aagcacataa ttttatatct caaaatatta
      601 attctgataa agtcttaaat gtttcagctt cagagtgttg cgagctttta ggcgttcaaa
      661 attttgtgag cgaaaatctt aaagttgttg tgaaagcgcc gtggtcgggt agtggtaagg
      721 gtttgcaatg ggtgaaaaat aaattgagcg atagtcattt aggttggata agcaatattt
      781 tagaaaaaca aaaaagcgta atactcgaaa agcaatattc tgtggttcag aactttgcta
      841 tggaattttt atgtgccgaa aataaagctg agttttgtgg atattcatta tttgaaacag
      901 atagaggcag atttcaatat tgtgaattaa tgagtgatag ggaaatttta cacaagcttt
      961 gcagtctcgg cgttttagaa gaaagttttt tagaaattca aaaattactt ataaaattta
     1021 ttcaaaaaga aatttctccg cattattctg gaattttggg tgtagatatg tttttgtatt
     1081 ccgacaattc tgaaatatta attcatcctt gtgtagaaat aaatttacga ctgactatgg
     1141 gttatgttgc aagaattttg ttcgataaat tcataaacaa taccaaaaaa ggcaaaatgt
     1201 ttgttgaaca caatgcacaa aaaggctttt tttatgaaga taatttaaca agaaagcaaa
     1261 aaaatccttt aattattgaa aataataaaa tcctaagtgg ctatttatca ctaaataatg
     1321 ttttgcctga aacttcttac agaatttata ttgaggtttc ataacttgtt aattaaaaat
     1381 tgaaaaacat cttgaaatct tgtgttaaaa aaattagttt tggcagatta aattaaaaaa
     1441 cgatggaaaa aaaaatcaat tctgctctta tttcggttta tcacaaagat ggtttagaga
     1501 ttattgttag aactcttgaa aaacacaatg ttaaaattta ttctacaggt ggaacttggg
     1561 agtttataaa taatttaggt atagatgcaa taaaagttga agatattaca ggttatcctt
     1621 cgatttttga cggtagagta aaaaccttgc acccaaaagt ttttggtgga attttattta
     1681 ttcgcgataa tgaaactcat caaaatgaag tggaaactta tgaaattcct agttttgatt
     1741 tggtaatagt tgatttgtat ccatttgaag aaacggtgaa aaatagtaca gacgaagccg
     1801 aaataattga aaaaattgat attggtggca tatctttaat tagagcagct gccaaaaatt
     1861 ttaatgatgt tttagttgtt tcaaatcgtt ctcaatacca agaactgtat agccttttag
     1921 aggaaaatgc ttgttataca agtttagaaa atcgtaaaaa atttgcatta gaatcttttg
     1981 gaattagttc agcttacgat acagcaattt tcaattggtt tgataataat agttattcag
     2041 aatttaaagt ttctgaaaaa tcaagcacaa ttttaagata tggcgaaaat ccacatcaaa
     2101 aagcaaaata ttttggggat ttatcagcta tgttcgagca attgcatgga aaagaaattt
     2161 cgtacaataa tattttagat attgatgcag cagtaaattt aatttcagaa tttgaagaaa
     2221 caacatgtgc tatattaaaa cataataatg cttgtggaat tgcttctcga ccaacgctta
     2281 gcgacgcttg ggacgctgct ttagaagccg accctgtttc tgctttcggt ggaattatta
     2341 ttacaaatca aatagttgac caattaacag cagaaaaaat taataaaata tttttcgaaa
     2401 ttattatcgc acctgactat acaaaaactg ctttagaaat tttaaaacaa aagaaaaata
     2461 gaataatttt aattcaaaaa tacagagaat taaaagaaaa aacttatagg tctgttttaa
     2521 atggtgtgtt agtgcaagat agagatgcta aaattgaaac cgaagatgat ttcaacttta
     2581 ttacaaattt acgtcctaac gaagctcaaa ttgcagattt gctttttgcg aataaagttg
     2641 tgaaaaatac aaaatctaat gcaatagttc ttgtgaaaaa tcagcaatta ttagcaagcg
     2701 gcacagggca aacttcgaga gttgatgctc taaagcaagc aattacaaaa gcaagaagtt
     2761 tcgatttcga cctacaggga gcagttatgg cttcagatgc gtttttccct tttagcgatt
     2821 ctgtagaaat tgctcatgaa gcaggaataa attcagttat tcaacccggt ggttctatta
     2881 gagacgatga aactattgag ttctgcaata aaaataatat tgctatggtg tttacaggaa
     2941 ttcgccattt taagcactaa taacaattgt gaaaattgca ttttgttaat aaatatttgt
     3001 aagaatattt gttttgtata aatatattta atacttttgc accatagaaa tcccacgaaa
     3061 acgtggggtt tcttttattt tatagagttt taattaataa aaaataatca aataataata
     3121 tgtctgaaga gatttcttat ttaaccgaag aagggttaaa caaattaaaa gacgagttag
     3181 accgattagt gaatgttgaa agagggaaca tttcaagaca aattgccgaa gcacgcgata
     3241 aaggcgattt atctgaaaat gcagaatatg acgcagctaa agaagcccaa ggtttattag
     3301 agatgaaaat tagtaaactt caggaaattg ttagaaatgc gagagtttta gacccaagtc
     3361 aaattagcac agatgctgtg caaatactaa ataaagttaa actgaaaaat gttaaaactg
     3421 gctcaactat ggaatatact atagttgccg aaacagaagc taatttaaag gaaaagaaaa
     3481 tttctgttaa cacacctatt gctaaaggtc ttttaggtag aaaagttggc gatgtggtag
     3541 aaattgaagt tccaaatgga attgtaactt tcgagatttt aaacatttca atataaaatt
     3601 aaataatatg gcttcaattt tcacaagaat tataaatggc gaaatacctt gctacaaaat
     3661 agcagaagat gaaaatttta tttctttttt ggatataaat ccattaaaaa aaggacatgc
     3721 acttgtgatt cctaaaaaag aaattgacta tattttcgac aactctgatg aaactttatc
     3781 gaagatttta gtttttgcta aaaaagttgc caaaggaata gaagctaatg ttgaatgtaa
     3841 aagaataggc ttagtagtag tcggtttaga agtacctcac acccatattc atcttgttcc
     3901 tataaatcaa gaatcagatt tgagcttttc taacccaaga gtaaaacttt ctcaacaaga
     3961 atttgaagaa ttggctaaaa aaatctctaa atcaattagt taaaagttta aagagtattt
     4021 agtgttgagt ttttagtttt tagtagtgag tttttaaaaa catcacacta attgtaagta
     4081 gtatatttgc ttcgcatagt tcgatttgct cactctgctc gctctgatgt ttgttgtccg
     4141 taggacaaca taagaccatc aaaggcttgt ccgcaggaca acatgtgatt aaccgtaggc
     4201 ggcagcctcc cgctcgctcg gatttgtaat ccgagtgctt ggtaatgtta cactcggatt
     4261 gcaaatccga gcgagcatca gtttttttat acttgcttta ttgcttgact ttatgctgtt
     4321 tctatagaat ttaaaatcta ccaactaaag ttttttaatt tttctaagca ttacaatata
     4381 aaattcatta tctttaacat caaattattt ttaatgaatt tatttagaaa tatatgagta
     4441 aaaattcggc tgtctataaa cagttaccag cagtttccaa acttttggac tctgattcat
     4501 tatccgataa aattgaagaa tacggtcggc aggctgtaac agaatctatt cgtcgaattt
     4561 tagaaaatta tagaaaaatt ttaaaaacac aatctcaaga aatttccgaa aatcaaatta
     4621 tccaagatat ttgcgatgat attgaaaaat caaataaaaa ttctttaatt aaagttgtaa
     4681 atgcaagtgg agtaatcata aatacaaatc ttggacgtgt tcctttgagc gaaaaagttt
     4741 ttttagaagc tcgcgatatt gcttgtgctt acaataattt agaatttaat ttagaaactg
     4801 ctaaaagagg aaaacgaaat actcatttaa aaaatatttt atgcaaactt agtggtgccg
     4861 aagatgcttt agttgtaaat aacgcagccg ctgcggttct tctttgttta aatacttttg
     4921 caaaacgaaa agaagttttg gtttctcgtg gcgagttagt tgaaattggc ggttcttttc
     4981 gtattcctga aattatgaaa gcagcaggct gtaaaatggt agagattgga actactaaca
     5041 aaaccaagct ttcggattat gaaaatgcaa taagtgaaaa aacagcaatt atatttaaag
     5101 cacatcaatc taactttgta ataaaaggat ttaccgaaga agtcgaagtt tcagaattag
     5161 caaaacttgg aaagaaatat aatttgcctg taatttatga taacggttcg ggcttgttag
     5221 aaaaatcaga ttttaaggct ttgaaaaatc aaattgatgt aaaatcatct ataaaagcag
     5281 gagtagtctt agtgattttt agtggcgaca agctttttgg cggtccccaa gctggaataa
     5341 ttttaggaaa aacttctttt attgaaaaat tagaaaaaaa tcaattattg agagctttac
     5401 gaattgataa aactacgatt gccttgttag aagcaagctg taagctttat attaatgaga
     5461 aagatttata taaacataat tttattttca aaactctttc tcaaactcaa gtagaattaa
     5521 aaagtaaagc agaatttttg caaaactctt taaaagaaaa taaaatttca tctaaaatta
     5581 ttccaaacaa agctatgatt ggtggtggaa caaatcccga aagtgaaaca gaaagttttg
     5641 cagttaaaat tttatttaat gaaaaaaata aaaagcaaaa ttctgaaatt tctgctatag
     5701 tatataaatc tctaatgaaa aatcatattc ccataattgg aattttacgt aaaggtgaac
     5761 ttcttttcga tattattact tgcaacgaaa atgagttaac aacaatatct caagcacttt
     5821 gtcaagcaat gaataatact tctaaaaagc aaaaatgaag catttaatta ttggcactgc
     5881 cggacatata gatcacggaa aaacatcatt gatacaactt ctttcaggaa ttgattgcga
     5941 cacacacaaa caagaaaaag aaagaggtat tactataagt cttggttttt cacatgttgg
     6001 tttgccttcg ggtcaagttt taggaattat tgatgtgcca gggcataaag accttattga
     6061 tacaatgatt agtggagcaa gcagtattaa tttggttttg ttagtaattg cagctgatga
     6121 aggtattatg ccacaaactt ctgagcatct gaatattata aacgcacttg gaataaaaca
     6181 tggaattatt gttctttcaa aaattgactt agttgacgaa gaacttgttg aaattgcaaa
     6241 atcggaaatt aaagattttg tgcagaatag cattttagaa aattcgccaa ttattggtgt
     6301 ctcatctcat acaggcgagg gtaaagataa tttaatttta gaattagaaa attttgtaaa
     6361 atcttaccaa gaaacagagt ttaaggataa ttttagaatg tatatcgaca ggctttttgt
     6421 tgtgaaagga catggaagcg ttgtaacagg ctctgtttta gacgggaaat tagaaagtgg
     6481 agaagaggta tttttacttc catcaaactc gaaaaagttt agagtgcgag ctatggaaaa
     6541 atacggaaag ccaactgaga ttgtgcaaaa aggcgataga gcagcaataa atttaattgg
     6601 tttaaagcaa gaagatttta caagaggaat gctaattagc aaaaatgaga ttgtaagcac
     6661 aaaattgatt gatgtttata tttcaagttt tgatttagtc ccaacattaa aattgtggac
     6721 tgatataatt tttatttctg gcacttatca atcaatgggg aaaatgcact taattgataa
     6781 ggaaaaactt tttgcaggag acgatgctat agcacaaatt catttagaaa aagattttgt
     6841 tggcgaaata aaagatagat ttatttttag aaattcttca gatgatatta gtcttggcgg
     6901 tgggtttgtg attgataatc aaccacttca tcataagagg cgaaacttag agcttagtca
     6961 aaaacttaga aatattgcaa caaatctaat tcaaggcaat gaattggaaa gcaatatttt
     7021 aaggataatt gaaaaagaac cacgtccttt tggtattgat gagattgttg aaaaaacttt
     7081 gtcgagcaaa gaagaaattt tatcagaaat atctaatcta tctcaaaata tttgcgttta
     7141 taataacgaa attttgattt attctcgttt tgataaaatg tttgaagata aaataattgc
     7201 ttatttaaaa gattatcatt cagtaaatta catttttgat acaggaatgc aaagcgagga
     7261 aattttaggt aaatttaatc ttggaaagtc taatttggtt aaagattacc tcaaaaatct
     7321 tttagaaaga ttaaaagaaa attccttgct tgatttttat gaaaatactt ggatattaaa
     7381 aaatcataaa gtaaatattg accagaaaac tcaaagtgaa ttagattggt tagagtctga
     7441 atttttagca tacaaggacg aaaagcctgt attgttggat attgaagaaa aagcaagtgc
     7501 gaaaaatatc tcaacaaaaa aatacaaact gtatctgaat tattttgttt ctaaaaatat
     7561 attagtggtt tttaatggcg aatacatgca ttcaaaaatt tttattgaag ctaaacacaa
     7621 actgctaaat catctgaaaa ctaaagaaaa tgggatttat attcctgagt acaaagaagt
     7681 tattacaggt acaaaaaaat tcagaggttt aatttcagat attttagaaa atcaaggact
     7741 tataaaattt attaaggata gcaataacga atctgtttta ttaattactg ataggggtaa
     7801 gatgatttaa aagaattaat gcaattttag tcttctcgta aactaaattc tgtatctata
     7861 tcatcttgga agttaacatt ttgaactttc catttatttt ttgggcagta tagaactaca
     7921 gtaaatttaa ctggttgtct ttcaaatttt agagagtaat tatattttct atatgattct
     7981 ccagcttgat aaacattaat aatttcatat ccatgataat cgcccaataa ttttcgagag
     8041 agttctaatc tttctttaat tgattttatt ccaggttgat ttgggtctat catattattt
     8101 gttgcaaata aatagtcgat agcagctcca gcatcttctt caaaaatttg gaaaaaagca
     8161 tctattaaat cttcggctgt tgcttgagct ttaagatttg tgtttataca caagctaaac
     8221 acaattaaaa taaaaaatga ttttaaaata tttgccataa cattttattg taaagttaat
     8281 tcttaaaatt gctataaagc aaaattaaga aaataacttt gatttcgatt aaaaatattt
     8341 attttgttaa aacaatattt ttttcttcta taatctttct caaatttatt aacgcataac
     8401 gcattctgcc taaagctgtg tttatgctta catcggtttg ttcagcaatt tctttaaaac
     8461 tcattccacc ataatgtcta agcataataa tttctttttg ctcttcaggt aaaaattcga
     8521 ttaaagacct aatatcattt tcaatttgtt ctccaacaat ttggtcttca atgttttcgt
     8581 gggaaaattt ctctgaatta aagacatcta ctgtgctgtc gtcatttgaa aaagtgttta
     8641 aacttttttc tcttctaaag tggtcgatag tgaggttgtg ggctattctt attacccaac
     8701 tagagaattt acctttatca acatatttac cttgttttag agaacttata actttaataa
     8761 aagtttcttg gaataaatcg tcagcaatgt gagaattttt cacattcata agaatgtaag
     8821 aataaatatt tttcttgtga cggtgtatta gagtttccat acattcttta tcacccttaa
     8881 tgaatctttc gattaattcc tggtcggaaa aatttttaag cttcataata tttagtttta
     8941 atgtttaagg gtagaagtct aatctatagt atggtctcct ttctttttta gtttatttta
     9001 attatctttt tatacgttta attataaata aggttacaaa aactatgcaa atttaatgaa
     9061 aatctttatt ttccaacaaa attacgtttt atttttcaca aattccagtt aattggctca
     9121 attttgtgtt tttttagata tttatttgct tgcgaaaaat gtttacatcc caaaaatcca
     9181 ttataagcac taagaggcga ggggtgagct gcttttaata ttaaatgttt gttttggtca
     9241 attaaatttt cttttgcacc tgcatatctt ccccatagta taaaaaccaa attattcttt
     9301 tcttgactta atgtttgaat aactttatct gtaaaaattt cccagccttt attttgatgc
     9361 gaacctgcat tgtttgctct cacagttaat gttgcgttga gtaaaaacac tccttgtttt
     9421 gcccatgctt ctaaatttcc gtgatctggg atattgaaat ttatgtcttg ttttaattct
     9481 ttaaaaatat ttactaaaga aggcggtggc tttactccct tgggtacaga aaagcacaat
     9541 ccatgagctt gacctggtcc gtgatagggg tcttgtccta aaattacaag ttttacatct
     9601 ttaaatggag ttaaattaaa tgcgttgaaa atttgcgaac cttttggata gacagtaaat
     9661 ttattttttt cttcaattaa aaattttttg agatttaaaa agtattcttt attaaactct
     9721 tcttttattt gtgcatacca agattcttca atattaacgt ttgtgatttt cattattcat
     9781 tatcagaatt ttcaatatgt ttaaataaat tatcaagttg gtttgtgtct attatttttt
     9841 cgttagtaat ataaatattt tcgtgtaaaa aatttccgta ttggttaatt tctttaatga
     9901 tattttcgat taaatccaca atgtcaacat gcagtgcaat caatacagtg cctattgcgt
     9961 atttgccagt aaaagataat tcttctagga tagaatcatt ttttgaaaaa gaatattcaa
    10021 tttcgccttt aaatatattt tgttgagtgt ctgtaaaatt ttcttttgca ataaagcaaa
    10081 aagcatatct tagtctatgt cctttacctc ctaagcccga agcaatataa attttatttt
    10141 catctaaaag gtttgcttct aaaaacattc ttgcttgttg gtaggctaag aatgcccagc
    10201 tttgcagttc tttttctggg ttttccaaat atttttcaat cattctaaag atatcaactt
    10261 caccgaatga tgcaagaaga gcaaggcttt tctttttttc ttcaaggcta gtttctgcct
    10321 tgtttaggtt ttcccaaatt tgttcgggtt ttggaatgtt atcctcttta gtaaaacttt
    10381 gcagagtttc aaaaaactct aattgcattt cagctcctac atttgtttct atgatatgaa
    10441 ttttttcagg attgagttga gcaatttctc tcattttatt tatcattttt tcctgtctat
    10501 tcatattaga gtttttattt taataaatat tttattatta aataaattaa ttatagtatt
    10561 tgatctgaga gttttaattt cagattattg tcttatgcta agaataactt taggttattt
    10621 ttttgtatct tctattttaa taacttttcc aacgaagaat aagtcttctc cagcaagtgg
    10681 gtgattgaag tctagtacga cagtgtcgtt ttcaatttca ataactatac cgtcaaaaat
    10741 atttccttct tcatcttcca taggaacata gtctccaact tcaggaacac cttcttcgtc
    10801 ttcttcaaaa atatctatag gaaattcagc aatagcatct tcgtcttcgt caccataagc
    10861 ttggtctttt gtcaaagaaa atttaaagct atcaccttct tttaatccta ttagtttttc
    10921 ttcaaaagtt tctaacattt cgcctgcacc acataaaaat attgcaggtt cgtcttcttt
    10981 tattgattct attaattcac catcataact atctacgttt aaatcgtata ttatagttac
    11041 tagtttgcca ttttcaactt tcatattatt tgtgtttata aaatattatg taatgatatt
    11101 gataaattta ttaaaatgca agtaaaatat aatatttttc aatttttatt ttgcttcttc
    11161 ggttttattg gtttcatcag tttcttttgt ttcttctgtt tcatcaactt cagtatcaat
    11221 aactttagca tcgatttcta aggtttcttt atcttgtttt ttatcctttg catattcgct
    11281 tcttagccaa aatgacatca taatattgct aagtccataa acactgaaaa ctattccaaa
    11341 gaaaacagta atatcttgga tgtttttgaa tggatttata ataaatatgg ctcctgctat
    11401 tagcatgatt gcagctatag ctataaacat tttaccgaaa ttatttagag gtttaatgtt
    11461 taaagttgtt attagatgta aaactcctgt cattattagc aatacaccta ataatattaa
    11521 taaagcttta ataaaaaaat ttgggataat tattaaaact attccaaacg ctatgtttat
    11581 aaggcttaaa attatttgag aaattttaat aaaagaattt tcaggttttt ttctgagtgt
    11641 ataaataaaa attcctaatc cacccgatgc tattaatgca ccaataataa taaccatggt
    11701 tttcattgag aaatcaggaa tactaagagt tgcaattcca aaaccgaaag caattaaacc
    11761 aatgtaaaaa aataaatttg gtgatgtttt tttagttttc atattttaat attttattgc
    11821 aaatatataa aatttataaa aacaaacatg catctccata acttaaaaac ctaaaattat
    11881 tagttagtgc atattcataa acttctttcc aagattctcc aataaatgca gctaacaaaa
    11941 gcaataaagt tgattgtggt tgatgaaaat ttgtaattaa ttgattggta aattgaaatt
    12001 tatagcctgg cacaatcatt atggacgttg aacaatttat ttgttcaaaa ttattttcat
    12061 tcatccaatt tagcatattt ttcaatagag tttctgttgg aatattttcc tttttctctt
    12121 cgtatgcttc ccattggcta atttcaaaat agttgtcttt tttatttttg ttaaaatata
    12181 tttgcacagc tatatgatac aaactttcta aagttctaac agttgtagtt cccacaacta
    12241 ttgtttttcc taagtaagct agtaagtttt cgatattaga ctttgaaaca ataaaatact
    12301 cggtgtgcat tttatgctca gctacagtag tggtttttac aggttgaaaa gtgcctgctc
    12361 caacatgtag agtgatatta tcagttttaa tgtttttagc ttctaagttt tgaaaaacag
    12421 tttctgtaaa atgcaatcct gctgttggag cagctacaga accttgcttc tcaccataaa
    12481 tagtttggta tctttgatta tcaatacttt cactttctct attcaaatat ggtggaattg
    12541 gaacattccc agaattttct aaaatttgat aaaaaattat attttcatta tcccaagtaa
    12601 attctatttc aaaggcattg ccaagttgtt gtttcttttc gatttttaat tcaaaatttt
    12661 gggaattaat gtttatgttt tttttcaaat ttccagattt ccatttttta ttatttccaa
    12721 ctaaacattt ccatgtacat tgctttgtag aatttaacgc atttgaataa ttaataggta
    12781 tgggttcaag acaaaaaatt tctattatag agcctgtttc tttttgaaaa aataatctag
    12841 cattaataac ttttgtgtta ttgaaaaaca ttaagctatt tgtaggtaaa aaatctgtaa
    12901 tatttttaaa cacagactgt gatatagaat ctttattttt tatcaaaagc attgattcgt
    12961 ctctgtttgc tttaggatat ttagcaattt tgttttctgg taaatcgtag ttgtaattat
    13021 taatatttat ttgtttgtaa tctttcattt aagtaaattt gcacaaaagt aagtaaaaac
    13081 tttgattatc taaaaataca aataaactct tgaaaattat aaaaaatgaa aataaatata
    13141 ggcgacaaag ttagattttt aaatgatgta ggtggtggaa ttgtggttaa attcttaaaa
    13201 aacaatatgg ttttagtaga aaatgaagat ggttttgagt atccaattaa ttctaacgaa
    13261 atcatagtgg tggaaaaagc aaagccacca gagcctgccc aaacaaacaa agtaagcgac
    13321 aaggttatag agcagaaaaa tgaactaaaa gttaagttaa aaaaggataa attaacaaat
    13381 attatttttg gttttgtaag gaatgcagag gagcaacaag atggttttga ttgttatttg
    13441 attaatgatt caaactttag tcttttttat catgtagttt taaaattcga tagtggatta
    13501 aaaaaaattg atgcagaaat tttagaacct aatacaaaaa tttatgttac tcatttgagc
    13561 cgtgatgaaa ttaattctgg caaggagttg ataattcaaa ttttgttttt tgataaccca
    13621 catcaagttc ttcgtccaca aattgaaaga aaaataaaaa tagttccttt aaattttttt
    13681 caagaacatt tatttataga gaatgatttc cttgatgata aggcttatat ttttgagttg
    13741 ttgagagacg atggaagttc gcttaaatct gagaaaacag cgaaagattt ggaaaaaatg
    13801 tttttggaaa aagaaagtgc cgaagaagat aagtcaaaac gatattctgc tcgtcaagaa
    13861 agaaaaacca tagaagttga tttacatata aacgagttgg tagagagtgt tgttggtatg
    13921 acaaatgctg aaattttgga gcagcagatg aaagtttttc atcaaactat gacagatgca
    13981 attatgtcta atgctggaaa agttattttt attcacggta ttggaaatgg aacattaaaa
    14041 gcgacaatta gagagtcttt agcgaaacaa tataaggtaa tatttgaaga tgcttcgttt
    14101 agggaatatg gttttggggc aactatggtt ttattgtaaa aaacttttgc attattaata
    14161 tgaaatttgg attatttgtt gaaaatttaa aaatatcgat aaattcaatt cgctcaaatt
    14221 tgttgcgacc agttttaaca gtattaataa ttgcaatagg aataatggct ttggttggaa
    14281 ttctgactgc cgttgagtca atcaaaacta ctataagtag tgagtttcag agtatgggag
    14341 caaatgtttt tactattaga aggcaatcta gtgagcgaca tggtggtgga tttagacgag
    14401 ttgagcgtaa aaacattagc tatgaagatg ctactttgtt taaaaatcaa tattcttttc
    14461 cagctcgagt aagtattatg acaaatagtt cgggctctgc aacagtaaag tatgggaata
    14521 taaagtcaga tcctaatata agtgtttatt ctgttgatga tgaatatttg tttacatctg
    14581 gttatgagat agataaaggc agaaattttt caagtcagga agttttatca ggcgagaata
    14641 ttgttataat aggtagcgaa ttagcaaaat tattatttat agatagcgag agtggtttgg
    14701 ataaatttat ttctataggc tcgcttcgtt atcgagttat tggaatttta aaagaaaaag
    14761 gcacagcatt tggaggttct ggcgataaat tttgttggat tcctcttcaa aatggtcgtc
    14821 aatattttac agcatcgcaa aacacttata ttattagtgt tatgcctcac gatatttcta
    14881 atattgattt agcaattgga gaagcagaag gattgtttag aagtgttaga aaattaagtg
    14941 tttatgatga gaataatttt gcaattagaa aaagcgacag tttagcaaat atgcttatag
    15001 agaacctttc tgttgtaact attggagcca caataattgg attaattact ttactaggag
    15061 cttcaattgg tttgatgaat attatgttag tttctgtttc ggagagaaca cgagaaattg
    15121 gtgttagaaa agcattagga gcgagttcta ctgcaattaa acagcaattt ttgtttgaat
    15181 caatttttat tggacagata ggagggatat taggtataat tttaggaata attattggaa
    15241 atttaatttc tttattggta ggtggctctt ttgtggtgcc gtggttgtgg gttttaatag
    15301 gagttgtttt gtgcttttta gttggaatag gttctggtta ttttcctgca gttaaggctt
    15361 ctaggttgga tccaatagtt gctttaagat atgaataaaa aaaagctgtg tcaaaacagc
    15421 ttttttcatt aaaatatttt taaaagaagt aattggctta tttgaaaagt tttgctacta
    15481 attctaccac tctgcaagaa tatccccatt cgttatcgta ccaagtaatt gtttttaaag
    15541 ttcttccatt cagcatagtg caacctgcat caaaaattga agaatgtgta ttgtgaataa
    15601 tatcaacaga aactattggt tcttctgtgt attgtacaat tcctttcatt ggtccttcgg
    15661 cagcttcttt aattgcttga tttacttcgt ctattgtagt gtcagttttt aaattaacaa
    15721 ctaaatcgac tgaagaacct gtaggagtag ggactctcat tgccatacca tcaagtttac
    15781 cgttaagttc tggtaaaatt ttacctactg ctttagcagc acctgtggta gtaggaattt
    15841 gagaaatagc acacgaacgc gctcttcgta aatcactgtg tggtccatca agaataattt
    15901 ggtcgtttgt gtaggagtgg attgttgtca tcaagccatt ttctatacca aatctgttgt
    15961 ttaaaatttt tactattgga gccatgcagt ttgttgtaca tgaagcatta gagacacatt
    16021 gaacttctgg agttaatttt tcatcattaa cgccaagaac taccattgcg tcaatattat
    16081 cttttgctgg tacagttaaa actacttttt tggcaccatt tttaatatga tcgccataac
    16141 ctcctttagg actttcttta gatctaaaaa ttcctgttgc ctcaacaact acgtctggag
    16201 cggtgggcca aggaatattt ataggacttc tttcagcact tacttttatt tctttacctt
    16261 ctacgattat agaagtttcg ttgtaagaaa tatttctatc aaatattcct tgcgttgagt
    16321 catactttaa aagatgtgct agagtttttg tatctgttag gtcattgatg ccgattattt
    16381 ccatatcatc tctatcgaat gcaattttaa aaacattgcg tccaatacga ccaaatccat
    16441 tgattgctac tttaattttt gtcataatgt aaattttaat ttttacaaaa atataaaaaa
    16501 atattgaaat taatcaagat tttttggata aaaaactgat gttatacaac aaattaatat
    16561 tttaattttt tgataatttt ctttgttctt ttctaaattc acgattcaat ttagcatcta
    16621 atttattgcc aaaatagtag caaataaata ggtttaaaaa gttagagtct tttgtgccag
    16681 acataatttc tatagggatt aaataatttt cataaacaaa tcctgtttct aaaactttag
    16741 acttcattat gttttgacta aaatcaaagt ttaagccaaa tttcaaataa aagcctggat
    16801 gaaactttgt ttcttttaat cctaaagtaa acggggctct accaattata tctgttggat
    16861 tgttttgaat gtcaacattt aatttatgag tagaagtata tgggtaaagt tgcttgttat
    16921 ttactacttt tacactgtca actatttcat aataaattgg tttgctagct gcaatagcaa
    16981 ctccaccaga tagtgctaag aatattgaaa tactattttt atctcttttt tcgaataaaa
    17041 ctctgtttat tccatagccg aacttagcat tgtgcacaga gtgagttttt ccaaacacaa
    17101 atttttttat atacaaactt tgaaaatatg gatttataac tttaagttct tttggacttt
    17161 tcatgtaatt atactcagcc tcaaagaatc tactataatt acgatttatt tttgtagtat
    17221 gactatagta tccaccaaat ccattagaat ttagtttaat tccaaaagaa tttacaaaaa
    17281 atttgtctgg ttctcttgaa gtttctaatt caccttgtgc aaagctagag cctaagcata
    17341 agtacacacc aaatataaat agtaataaat atttcatttg aaatttatta ttactgcaaa
    17401 aataaataat tttttattta tttaaaatta ggctattgtg ttttctgttt caatattagc
    17461 ctttccatat gcagggcaat cttctgaacc tttacaagaa cttaagccta ttgctattaa
    17521 tactatagca agaattataa gtattgtttt tttcataatg aattgatttt atttcacaaa
    17581 gttatcaaat gttttgtaat aataaaaatt agttataatg ttttattaac aaattttagt
    17641 ttgtaaaaaa agggagtaaa aaactccctt ttttaaatgt tttgttgtgt ttattattgt
    17701 tttacaattt ttgttgaata agaattatct tgtgttaaaa cttttaaagt ataatttccg
    17761 tttgcaaaac tagacatatc aatagtgtcg ctttctgtac taaacactag acgacctaat
    17821 gaatcataca aactaatatt ttcaatattg atagcattta ttttaataat tccagttgtt
    17881 gggtttggat atacatcaaa catttctgct ttttctattt ctgcatttac accttggtga
    17941 agtgtaacag taacttggtc gctatcttcg cagccattaa ctgtaattgc agtaacacta
    18001 attacatttg ttccaaagtt taaattatca gcacttaata ttacaaaatg ttctgtatat
    18061 tcaggaccta gagaccatac atagctaata catccatatc ctgcagttaa aatagcagta
    18121 tagttttcat acatttcaaa atcgtctcct aagtcaacta ctggcatagg ataaatttcg
    18181 atgttaagag aatcttttgc ccagcaaccg aattcgtcag taacagttac agcataaaaa
    18241 cctgattttt caattgtaag agattgtgta gtttctccag tattccaagc gtattggcag
    18301 ctgtcagctt gcacagtaag tgttcttgtt tcttctgcac aggacacagg catcgctaag
    18361 gcaagaacaa tggaa
//
LOCUS       DAOPNX010000048        18242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_3682_length_18242_cov_6.208611, whole genome shotgun sequence.
ACCESSION   DAOPNX010000048 DAOPNX010000000
VERSION     DAOPNX010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18242
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3682_length_18242_cov_6.208611"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /gene="mazG"
                     /locus_tag="PKN53_06655"
     CDS             complement(<1..725)
                     /gene="mazG"
                     /locus_tag="PKN53_06655"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOD88665.1"
                     /translation="MDKLRKECPWDSVQTNESLRTLTIEEAYELAEAISKENNNEIKK
                     ELGDILLHIVFYAKIGEEKESFDIYDVIKNINDKLEFRHPHIFGDVKVKTAKDVEENW
                     ERLKLKEGKGKTVLGGIPEALPAMIKAYRIQDKARGVGFDWTEKEQVWDKVKEEYFEM
                     QEELKNNNSEKFEEEFGDFLFALINVARLYNINPENALEKTNKKFITRFNYLEQKTIA
                     QGKDLKNMTLDEMNEIWEESKAI"
     gene            complement(819..1391)
                     /gene="rsxA"
                     /locus_tag="PKN53_06660"
     CDS             complement(819..1391)
                     /gene="rsxA"
                     /locus_tag="PKN53_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOD88666.1"
                     /translation="MEYILLIISAIIVNNVILVQFLGICPFLGVSNKVSTALGMAGAV
                     TFVIVLATMVTYLLYQYILVPLEIQYLQTIAFILVIAALVQMVEIILKKISPSLYQAL
                     GIYLPLITTNCAVLGVALLVIQKDFNLLQGVVYGASISLGFGLAITILAGIREQLDTL
                     NIPKGMRGVPIALVIAGILALAFMGFTGIV"
     gene            complement(1397..1972)
                     /locus_tag="PKN53_06665"
     CDS             complement(1397..1972)
                     /locus_tag="PKN53_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOD88667.1"
                     /translation="MNQWKNFTKGLIKENPILVLVLGTCPTLATTSSAINGVSMGLAT
                     TFVLVGSNVVISLVGNLIPSKVRIPAYVVIIASFVTIIDLAMQAYTPAIYETLGIFIP
                     LIVVNCIVLGRAEAFASKNTVVSSLFDGLGMGLGFALALGFLGTIREILGTGKIFGLE
                     LMNQGALVFVLAPGAFFALGYLIALVNKLKK"
     gene            complement(1972..2637)
                     /locus_tag="PKN53_06670"
     CDS             complement(1972..2637)
                     /locus_tag="PKN53_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOD88668.1"
                     /translation="MAKKDSFVNMVVTLFVITAIPAVLLGFVYKFTKEPIEQAKKLKL
                     SNSINLVVPGASTAEIKEFNVMPETGKDSLTFFEVVSNNEIIGTAVQTYSDNAFGGRI
                     VVMVGFKPDGTIIDNDVLEHSETPGLGDKASKSKSDWNSQFNDKNPKEFNIKLKKDGG
                     EVDAITAATISSRAYVDALQRAYEAFIKYYNSSDIEESKVIEEIDEQTELETNETEKG
                     GVE"
     gene            complement(2640..3650)
                     /locus_tag="PKN53_06675"
     CDS             complement(2640..3650)
                     /locus_tag="PKN53_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOD88669.1"
                     /translation="MTINRLTVSMSPHAHGKDSVRKIMYGVIIAMIPAFLVSVYYFGL
                     DAIRVSLIAVASCVFFEWGIQKLFFKAKKTTITDGSAIITGMILAFNLPSNLPWYIIV
                     IGALVAIGIAKMTFGGLGNNPFNPALVGRVFLLISFPVEMTSWPKVNVLNFAMTDAET
                     GATPLSIVKEGLKNGESFQSIIDGMPHFYTDALMGNVGGSLGEVSAFALLLGGLYMLF
                     RKIITWHIPVAVLGSVAIFSAILYGVDPVHYASPAFHLLTGGIMLGAIFMATDMVTSP
                     MTKKGMLVYGVGIGVLTIVIRTWGVYPEGISFAILIMNAFVPLINKGFKPKRFGETIK
                     KA"
     gene            complement(3653..4984)
                     /gene="rsxC"
                     /locus_tag="PKN53_06680"
     CDS             complement(3653..4984)
                     /gene="rsxC"
                     /locus_tag="PKN53_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOD88670.1"
                     /translation="MLKTFPLGGIHPKENKFSAGKKIETIDLPKIVSIPISQHIGSPA
                     EPIVAKDDTVKVGQLIAKSSGFVSANIHSSVSGKVLKIDKVLDSSGYKQNCIIIEVEN
                     DEWLEDIDRSKDLVDNLDISAEEIKNRIIKNGIVGMGGATFPTHVKLSIPPGKTCKHL
                     IINAVECEPYLTADHQLMLEKADEILVGCKLIQKLVDAEKVIIGIENNKKDAIQLLTE
                     KAKKYSNIEVQPLKVKYPQGGEKQLIKALTKREVPSGGLPIDVGCVVQNVGSVFAIYE
                     AVQKNKPLIERVVTVTGKSVSNPKNILTRIGTPSDMLIAAAGGLPEDSGKIVSGGPMM
                     GKALASNNVPIAKGTSGILILPQEKASRPECFNCIRCGRCVSVCPMGLEPYLLKNMAE
                     VSMFEELQKERVLDCIECGSCSFTCPASQPLLDYIRLGKANLNKILRAKKS"
     gene            complement(5018..5875)
                     /locus_tag="PKN53_06685"
     CDS             complement(5018..5875)
                     /locus_tag="PKN53_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007481243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster domain-containing protein"
                     /protein_id="HOD88671.1"
                     /translation="MEIIINTLITLSLLGAILAVILYFVAQKFKVVEDPRIDTIQELL
                     PGANCGGCGYPGCRGFAETVVKNGSLDNMNCPAAGSEGMKKIAEAMGLSVEEKEPQIA
                     VVRCNGSFENRKKTRIFDGPSSCAIEHSLFSGDTDCAYGCLGLGDCVTACPFDAIHID
                     EKRGLPVVDEEKCVACGLCVKACPKNIIELRNKGKNNRRIYVSCINKDKGADAKRACD
                     VACIGCGKCAKECKFEAISIENSLAYIDFSKCKLCRKCASVCPTGAILEINFPQKKEK
                     LEENETLVN"
     gene            complement(5888..6307)
                     /locus_tag="PKN53_06690"
     CDS             complement(5888..6307)
                     /locus_tag="PKN53_06690"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOD88672.1"
                     /translation="MNTKSTIEHDGTVIDINDGKITVELTVLSACATCHSRSLCSFDS
                     SQKTIEIFDNNNISYKIGETVKVIMRQSLGGKALFLAYILPFFVILIALIICTIIGLS
                     EALAGIISILTLIPYYTLLYLFKNKLKKEFSFSIKKI"
     gene            complement(6442..8136)
                     /locus_tag="PKN53_06695"
     CDS             complement(6442..8136)
                     /locus_tag="PKN53_06695"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOD88673.1"
                     /translation="MNKIIFSVFFIFLNLGLFAQREFTEAERSKYHMNSPEEFKTSYR
                     NRAFSYTAPPVGGTRSIAEFEPNQGVIIAFPNNFAVPYSLISQLSQYVKVYIVCSSFA
                     QSYASSQLQNNGVNMENVSFVNAPIDSQWTRDFSPIFIEYGENNKVGIVDFPYNRPDR
                     TNDDNLPIVLGEYWNMEVFGMNLVHTGGNYMTDGLGIAASCDLVYTENPSLSQQEVSQ
                     LVQDYIGIENYHLVNDPLNDYIEHIDCWGKFLAVDKILITRVPASDSRYAEFEAMADY
                     WANQTTSYGNKYKVFRTNWGMDRNAYTNSLILNNKVFIPFNTGNPGAFNADAATVYEE
                     AMPGYEIIGIEYNKWFSTDALHCRTHEVPDFEMLKIAHLPYVEEINFQETYDFNAEIY
                     SFNNSNSITEVKLIYSVNNEEFVELEMENISGNTYNISLNNLNQGDTIRYYIQAKDNR
                     PKTETLPYIGQADPFSFTIKSTSNTTELTKDQYIKAFPNPATNILYFISNNLPNDNYE
                     IEIYNQFGQIIKKLNVEITDINWEISGIDISDLKSGLYSIIAKSGKTIFTTKFIKL"
     gene            complement(8147..11563)
                     /locus_tag="PKN53_06700"
     CDS             complement(8147..11563)
                     /locus_tag="PKN53_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOD88674.1"
                     /translation="MKQIILSFTLFFLSLTLFSQVNLVKLDSQLSENFSINVLEQNSQ
                     GLTLKLTNNSYYFKDVNTPRGESVIVRSPKARNNFPKGSPDLPIIPTSIMIPNQGGFS
                     YNIISSEYVTVENIEIAPSKGTIYRNQDPETVPYEYGREYFVDDFFPQNSAFISEPFI
                     VRDARGVNISFFPFTYNAIQKELRIYSEIILKITFTNDNFPNQIVNNNSKVSYEFSKL
                     YERHFVNFKSSTKYTPIEEGTPGKILIIAKDAYASAMTPYIKWKLEKGIETEMVLIST
                     IGTNSNDLKNFIQNYYNNNDLTYVLLVGDAEDIPTINVGSNYTDNGYTYLAGNDKYAD
                     IFIGRFSGNSLVDIETQVQRTVTYERDLNASDTWLTNGFTSASSEGAGQGHNGESDVQ
                     HMNIIKNKLQNYGYTITSVNQQGGNNTQISNHFNAGTGIANYIGHGDETLWVNTEFTN
                     SHVNALTNENKLPFIWSVACVNGHFKGRTCFAEAWLRAQKNNQPTGAIAFLGSTINQS
                     WTEPMTAQDEMIDILMETYASNIKRTFGGLSFNGMFKMIEAGGEGQSMADTWTIFGDP
                     SLMVRTKTPQAMNISHNAVMTIGETSFQVNCDEENALVSITKFNEDVTTILGTGYVNQ
                     GVATINFESLTEPGVVLLTITAFNKITYQEEILVIVPSGPYVIHTEYSINDEEGNNNG
                     LADYNETIYINETLKNVGVDVANAVVANLSIVDENLGTILEGQKSFGQINADETKTEN
                     NAFKLSIVDGIADQTNIKTILTITDGNANEWTSNVFIPVNAPKMKLAFNAIDDSEFGN
                     NNGSLDTGETFKIKIQAKNIGHASSVAGTVSISSESEYVSSINNSIYNISSLEENQDV
                     ITEFDISLAEEIPSGTTIYFDFKLIAGMYLDSLSLGFSVGEQIEDWESNTMTSFNWEN
                     NSNFPWTIVTDEVYEGEYALKSGTPTGGGESSLIINLQVLNDDQIEFYKKVSCEPVMW
                     GFMWDHFAFFIDDAEQGAWAGEVAWSKETYNVSAGQHEFKWSYIKDAYYNQGQDCAWL
                     DNIKLPPHQNVVAVMNTKSNILENSIEIYPNPAQDIAYLNLNMKENSSVNVRILNLSG
                     QIVYEFANPINIYSGENNILINTQNFTNGVYIVTVSTNQETLNSRLIINK"
     gene            12011..12085
                     /locus_tag="PKN53_06705"
     tRNA            12011..12085
                     /locus_tag="PKN53_06705"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12046..12048,aa:Pro,seq:tgg)
     gene            12177..13628
                     /locus_tag="PKN53_06710"
     CDS             12177..13628
                     /locus_tag="PKN53_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOD88675.1"
                     /translation="MINARIIMFFLGSLLIAESVFMLIALAVSVIYSGIDFNYLLISA
                     TATCLAGLLLVLPNLKIDKKVEKRESYLIVSLAWVLMSLFGALPFFIGGFIPSFTDAF
                     FETMSGFTTTGASILNNVEALPHGLLFWRSLTHWIGGMGIIVLSIAVMPMVKSGGVQL
                     FVAEAPGISPDKLHPRIKETAKKLWLVYVSLTVVLTCLLHVFGMNWFDSINHAFSTMA
                     TGGFSTKQASIAHWTSPSIHYIILLFMILSGINYSMLYFAFTGKIKKLIKNTEVKFYL
                     FLIIGFGLLVGLGLWIYMDLPLEKAFRDGMFQVVSVMTTTGFTTNDYMQWHPIGLWLI
                     LLLVMLIGGSAGSTSGGIKVVRIHILLRNTALEFKRLLFPSAVLPVKYNKTVLSDKTV
                     NSVLAFVILYVVLIILGTFVLSFTGLDFTSSLGAVVSSIGNVGTSLGETGPAVGFAGV
                     SAFGKWVLSFLMLVGRLELFTVMILFTKSFWKK"
     gene            complement(13639..14259)
                     /locus_tag="PKN53_06715"
     CDS             complement(13639..14259)
                     /locus_tag="PKN53_06715"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOD88676.1"
                     /translation="MAEFFRMHPDNPADRHINRIVEILKDDGVIIYPTDTVYGIGCDI
                     TKPKAVERVALIKGIKPEKANFSFICYDLSHISDFTKPLSNNIFKLMKRNLPGPFTFI
                     LEANNNVPKILKANRKSIGIRVPNNNIIREIVKALGNPILTTSLRTNDEIIEYNTDPE
                     LIYEEYENIVDAVIDGGYGGLIASTIIDCTGSEPEIIRQGSVEPEF"
     gene            complement(14261..14917)
                     /gene="fsa"
                     /locus_tag="PKN53_06720"
     CDS             complement(14261..14917)
                     /gene="fsa"
                     /locus_tag="PKN53_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOD88677.1"
                     /translation="MKFFIDTANLDQIKEAQALGVLDGVTTNPSLMAKEGISGKQNIL
                     NHYLEICKIVDGDVSAEVISTDYEGIIKEGEELAALHQQIVVKVPIIKDGVKAIKYFS
                     DKGIRTNCTLVFSVGQALLAAKAGATYVSPFIGRLDDNSTDGIELIGQIVDVYNYYEF
                     GTQVLAASIRHTMHIIKCLEMGADVATCPLSAILGLLKHPLTDIGLEQFLKDYNKLNK
                     "
     gene            15193..15873
                     /locus_tag="PKN53_06725"
     CDS             15193..15873
                     /locus_tag="PKN53_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOD88678.1"
                     /translation="MLFLQVDSLQNVASEVASEETLSLFELATKGGVIMIILGMLSII
                     AVYLFIDRFIAIQKAQKDQKAFMNNIKNNIFAGKVDTALDLCRVQEGPLARMIEKGIS
                     RIGRPLSDINTTVENVGKQEVSKLEKGLPLLATIAGGAPMLGFLGTVTGMVSAFYAMA
                     NAGNNLEIGTLSSGIYEALVTTVGGLIVGILAYFAYNILVAKITSLVVQLEAKTTEFM
                     DILNEPVK"
     gene            15880..16284
                     /locus_tag="PKN53_06730"
     CDS             15880..16284
                     /locus_tag="PKN53_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOD88679.1"
                     /translation="MAIKTRSKISSETSMSSMSDLVFLLLIFFMITSTMIAPNALKLL
                     LPSSNSQTQAKPFTTVSIKDMNDGTYTFAVGMQICAETDVEQLLKKELIGQEDPYVSL
                     HVDKSVPMEQVVKVMNIAKNNNWKLILATQPE"
     gene            16288..17031
                     /locus_tag="PKN53_06735"
     CDS             16288..17031
                     /locus_tag="PKN53_06735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOD88680.1"
                     /translation="MSLKKHNIYGIIAAVIYTITVIILLFLLGFTTPLPLPPEEAILI
                     DFGGGNINAGASSSTNTSNAQNTSSQISSHDGVNTQANEEAPFMQSGTNPAENPKKNE
                     DETETQETTAPTNPNADRLNNLFGGGVFGGGTGSGTGGTGTGGGNPGLGTGGSGTGSG
                     PGGIGGGIGNRRILKKVEPVGKDNMVGKVVLKIIVNDKGNVTSVTLVSTNCNECVQLA
                     IDAVKQWKYEAKPGTGEQTGNVTVEFKQV"
     gene            complement(17035..17682)
                     /gene="elbB"
                     /locus_tag="PKN53_06740"
     CDS             complement(17035..17682)
                     /gene="elbB"
                     /locus_tag="PKN53_06740"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HOD88681.1"
                     /translation="MKNIAVILSGSGRFDGSEIHEATLTLLALSKNQVNYICFAPNKA
                     QYHVINHLTGEVSNETRNVLVESARIARGNIKDIKELKAKDFDALILPGGFGAAKNLS
                     SFAYEGENYTVDTEAEKVVKDFHSAKKPIAALCIAPVIISKVLGAEVTIGTDKATAKI
                     IEHVGGKHINKDYHEVAIDTKNLIVTNPCYMLADDIYKVWLGVEACVEAMLGLIK"
     gene            17845..>18242
                     /locus_tag="PKN53_06745"
     CDS             17845..>18242
                     /locus_tag="PKN53_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88682.1"
                     /translation="MIKKSIFILNILLIPFLGFAQTHLSNDFASPTEMNVVSECNFET
                     YTYNHNFGSDIPELKCNRMYPGVSSYFKFSTLENSNLTLKVQLETEKLFGLAFYTLEN
                     GTYVELKCDTYRSTTGELKIYEEEELLSTE"
BASE COUNT         5988 a         2965 c         2813 g         6476 t
ORIGIN      
        1 ttaatagctt tactctcttc ccaaatttcg ttcatttcgt ccaaagtcat gttttttagg
       61 tctttgcctt gagctatggt tttttgttct aaataattaa atctagttat aaacttcttg
      121 tttgtttttt ctaaagcatt ttcgggattt atattataaa gacgtgctac atttattaag
      181 gcaaaaagga aatctccaaa ttcttcttca aatttttcag aattattgtt ttttaattct
      241 tcttgcattt caaaatattc ttcttttact ttatcccaaa cttgttcttt ttctgtccag
      301 tcaaaaccaa cgcctctagc tttgtcttga attctatagg ctttaatcat tgcgggcaaa
      361 gcttctggta ttccgcctaa aacagttttt ccttttcctt cttttagttt tagtctttcc
      421 caattttctt caacatcttt tgctgttttt acttttacgt ctccaaaaat atgtgggtgt
      481 ctaaattcta atttgtcgtt tatgtttttt attacatcgt aaatatcaaa actttctttt
      541 tcttcaccta ttttggcata aaaaacaata tgtaataaaa tatcgcctaa ttcttttttt
      601 atttcgttat tattttcttt tgaaatggct tctgcaagtt cgtaagcttc ttcaatagtt
      661 aaggttctaa ggctttcgtt agtttgaaca ctatcccaag gacattcctt tcttaactta
      721 tccattatgt caagaagcct tttaaattcg ctaagtcgtt tatccataac aaaaaaagag
      781 gcaatggttt tgcctcaaaa tataaaatta aaaattattt atacaattcc tgtaaatccc
      841 ataaatgcta gagctaatat tccagctata acaagtgcga taggtacgcc cctcatacct
      901 tttggaatat ttagggtgtc tagttgttcg cgaataccag ctaaaattgt tattgcaaga
      961 ccgaaaccta aagaaatcga tgctccataa accactcctt gcaacaagtt aaaatctttt
     1021 tgaataacta aaagagcaac cccaagtaca gcacagtttg ttgtgattag aggcaagtat
     1081 attccaagag cttgatagag tgagggactt atttttttaa gaataatttc taccatttgc
     1141 actaatgcag caattactaa aataaaagca attgtttgaa gatattgaat ttcaagtggc
     1201 actaatatgt attgatataa taggtaggta accattgttg ctagaactat tacaaaagta
     1261 acagctcctg ccattcccaa tgcagttgaa actttattag aaactccaag aaatggacat
     1321 atccctaaga attgaacaag tataacgtta ttaacgatta tagcagatat tattaataaa
     1381 atgtattcca taatttttat tttttaagtt tattaactaa agcaattaga taacctaaag
     1441 caaaaaaagc tcctggtgct aaaacaaaaa ctaaagcacc ttgattcatt aattctaatc
     1501 cgaatatttt acctgtacca agtatttcac ggattgtacc taaaaatccg agtgctaaag
     1561 caaaacctaa gcccatgccc aaaccatcaa aaagtgatga aacaacagtg tttttgctag
     1621 caaaagcttc agctctacca agaacaatac agtttacaac aattaaagga ataaatattc
     1681 ctaaggtttc gtaaattgcg ggagtataag cttgcattgc taagtctatt attgtaacaa
     1741 atgaagcaat aattacaaca taagcaggaa tccttacttt tgaaggaatt aagtttccaa
     1801 ctagagaaat tacaacgttt gagcctacaa gtacgaaagt tgttgctaaa cccattgaaa
     1861 caccattgat tgccgatgag gtggttgcta aagttggaca ggttccaagt actagaacca
     1921 aaataggatt ttctttaatt aatcctttgg taaaattttt ccattgattc attattctac
     1981 tcctcctttc tctgtttcat ttgtttctaa ctctgtttgc tcatcaattt cttctatcac
     2041 tttactttct tctatatctg atgagttata atattttata aaagcctcat aagctctttg
     2101 tagagcatca acataagctc gtgaagaaat tgttgcagca gttattgcat caacttctcc
     2161 gccgtctttt ttaagcttta tattaaattc ttttgggttt ttatcattaa attgagaatt
     2221 ccaatcagat tttgatttag aagctttatc tccaagtcct ggtgtttcgg aatgttctaa
     2281 aacgtcatta tcaataattg taccatctgg tttgaaacct accataacta caattcgtcc
     2341 accaaaagca ttgtcggaat atgtttgaac agcagttcca attatttcat tattactaac
     2401 cacttcgaag aaagttagag aatccttgcc ggtttctggc attacattaa attctttgat
     2461 ttctgccgtg cttgcacctg gtacaactaa atttatgcta ttacttagtt ttagtttttt
     2521 agcttgttct ataggttctt ttgtaaattt ataaacaaag cctaaaagca cagcaggtat
     2581 cgctgtaatt acaaacagcg ttactaccat atttacgaaa ctatcttttt ttgccataat
     2641 tatgcttttt taattgtttc accaaaacgt tttggtttaa accctttatt aattaatgga
     2701 acgaatgcgt tcattattaa aatagcgaaa gaaattcctt cgggatatac tccccaagtt
     2761 ctgataacta ttgttaatac tccaattccc actccatata ctaacattcc ttttttagtc
     2821 ataggtgaag taaccatatc agttgccata aaaattgctc ctagcattat tcctccagtt
     2881 agaaggtgga aagctgggct ggcgtaatgt acaggatcaa ctccgtaaag aattgccgaa
     2941 aaaattgcaa cagagcctaa aacagctact ggaatatgcc aagtaataat ttttctaaac
     3001 agcatataca agcctccaag cagaagtgca aaagccgaaa cttcaccaag tgagccacca
     3061 acatttccca taagagcatc agtgtaaaaa tgtggcatac cgtcaataat actttggaaa
     3121 ctttctccat ttttaagtcc ttcttttact attgataaag gagtagcacc agtttcagca
     3181 tctgtcattg caaagtttaa aacattaact ttgggccaag aagtcatttc aacagggaaa
     3241 gaaattaata aaaacacacg tccaaccaat gctgggttaa aaggattgtt acctaagcct
     3301 ccaaaagtca tttttgctat tccaatagct actaaagcac ctataacaat aatgtaccaa
     3361 ggtagatttg aaggaaggtt aaatgctaaa atcattccag ttattattgc tgagccgtct
     3421 gttatagttg tttttttagc tttaaaaaat aatttttgta ttccccattc aaaaaacaca
     3481 cacgaagcta cagcaattaa acttactcta attgcatcta atccgaaata ataaaccgac
     3541 actaaaaaag ccggaatcat tgcaataatc actccataca tgatctttct aacggaatct
     3601 tttccgtgag catgtggaga catcgaaaca gttaatctat ttatagtcat ttttatgatt
     3661 tttttgctct taaaatttta tttaaatttg cctttccaag tcttatatag tcaaggagag
     3721 gttgagatgc tgggcaagta aatgaacaag aaccacattc tatgcaatct agcactcttt
     3781 ctttttgtaa ttcttcaaac attgaaactt cagccatgtt ttttagtaaa tatggttcta
     3841 atcccatagg acaaacagaa acacagcgtc cacatctgat gcaattaaaa cattctggac
     3901 gtgatgcctt ttcttgaggt aaaattaaaa tgccagaagt accttttgcg attggaacat
     3961 tgttgctagc caaggctttt cccatcattg gacctccact aacaattttt ccgctatctt
     4021 cgggtaagcc tccagctgct gcaattagca tatcagaagg agttcctatt cttgttaaaa
     4081 tgtttttagg attggaaaca gatttgccag taactgtaac aactctttca attagaggtt
     4141 tgtttttttg tacagcctcg taaatggcaa aaacacttcc aacattttgt acaacacagc
     4201 ctacatctat aggtaaacca ccagaaggaa cttcacgctt agttaatgct ttaattagtt
     4261 gtttttcccc accttgtgga tattttactt ttaaaggctg aacttcgata ttagaatatt
     4321 ttttagcttt ttctgttaaa agttgaatgg catctttctt gttgttttct atacctatta
     4381 taactttttc agcatcaaca agtttttgaa taagtttaca acctaccaaa atttcatcag
     4441 ctttttctaa cataagttga tggtcggcag ttagataagg ctcacattca acagcgttga
     4501 taatgaggtg tttacaagtt ttgccgggag gaatagaaag ttttacatga gttggaaaag
     4561 ttgctcctcc cattcccaca ataccatttt ttataattct gtttttaatt tcttcagctg
     4621 aaatatctaa attatcaact aagtctttac ttctgtcaat atcttcgagc cattcatcgt
     4681 tttcaacttc aataataata caattttgtt tgtatccact agagtcgaga actttatcaa
     4741 tttttaaaac ttttcccgaa acagaagaat gtatgtttgc agaaacaaaa ccagagcttt
     4801 ttgcaattaa ttgacccact ttaacagtat cgtcttttgc aacaataggt tcggcagggc
     4861 ttccaatatg ttgagatatt ggaatgctta ctattttagg caagtcaata gtttctattt
     4921 ttttgccagc cgagaatttg ttctcttttg gatgaatgcc accgagtgga aatgttttta
     4981 acacgttttc aatctcctat aattaattag tattgtttta attcactaaa gtttcatttt
     5041 cttcaagttt ttcttttttt tgtggaaaat ttatttccaa aattgcacct gttggacaaa
     5101 ctgaagcaca ttttctacaa agtttacact ttgaaaagtc tatataagct aaagagtttt
     5161 ctatgctaat tgcttcgaac ttacattctt tagcacattt gccacagcca atgcaagcta
     5221 catcacaagc acgttttgca tcagctccct tgtctttatt tatgcaagaa acgtaaattc
     5281 tgcgattgtt tttaccttta tttctgagtt caataatatt ttttggacat gcttttacac
     5341 acaaaccgca ggcaacacat ttttcttcgt caacaacagg taaaccacgt ttttcgtcta
     5401 tatgaattgc atcgaaagga caagctgtta cgcaatctcc caagcctaaa cagccatatg
     5461 cacaatcagt atcgccacta aatagagaat gttcaatagc acaacttgaa ggaccatcaa
     5521 atattctagt cttttttcta ttttcgaaac ttccattgca tcttacaact gcaatttgag
     5581 gttctttttc ttcaacactt aatcccatgg cttcggcaat ttttttcatt ccctcacttc
     5641 cagctgcagg acaattcata ttatctaaac tgccattttt cacaactgtt tctgcaaagc
     5701 ctctgcagcc aggataacca caaccaccgc aatttgcacc gggtaaaagt tcttgaattg
     5761 tgtctattct aggatcttca acaactttaa atttttgagc aacaaaatat agaatcactg
     5821 ctaaaattgc tcccagcaaa ctcaaagtaa ttaaggtatt tataataatc tccatatagt
     5881 aaaaagttta aatttttttt atactaaaac taaattcttt tttaagttta tttttaaata
     5941 aataaagtaa ggtataatac ggaattaatg tcaaaattga aataatccct gctaaagctt
     6001 cacttaaacc aataatagtg caaatgatta aagcaattaa aataacaaaa aatggtagga
     6061 tataagccaa gaataatgct tttccaccaa gggattgacg cattattact tttacagttt
     6121 caccaatttt gtaggaaata ttattattat caaaaatttc tatggttttt tgtgaactat
     6181 caaaactaca caatgatctt gaatgacatg tagcacaagc agaaagtaca gttaattcaa
     6241 cagtgatttt tccatcatta atgtctatta ctgttccgtc gtgttcgatg gttgattttg
     6301 tgttcatttt tataaaataa atattgcaaa tttaaaatat ttacattcta aatttttttt
     6361 tttttaaaat attattaaca atttgcttaa tttagaatca gtttaaattg ctttatgcaa
     6421 aaataggagt tgttgttgga tttataattt tatgaatttg gtagtaaaaa ttgtttttcc
     6481 cgatttcgca ataattgaat aaagaccaga tttcaaatct gaaatatcaa ttccagaaat
     6541 ttcccagttt atatctgtaa tttctacatt taatttttta attatttgac caaattggtt
     6601 ataaatttct atttcataat tatcattagg aagattatta ctaataaaat acaaaatgtt
     6661 tgttgctgga ttaggaaaag ctttaatgta ttggtcttta gtcaattctg ttgtgtttga
     6721 agtgctttta attgtgaagc taaacggatc ggcttgacca atatagggta gtgtttcagt
     6781 ttttggtctg ttatctttag cttgaatata atatcttata gtatcacctt gattcaaatt
     6841 atttaagcta atgttatacg tgttaccaga aatattttcc atttcaagtt ctacaaattc
     6901 ttcattattt acgctataaa tcagtttaac ttctgtaatt gaatttgagt tgttaaagga
     6961 atatatttcg gcattaaaat catatgtttc ttgaaaatta atttcttcga catagggtag
     7021 gtgggcaatt ttaagcattt caaaatcagg aacttcgtga gttctgcagt gaagtgcatc
     7081 tgtactaaac catttattat actcaattcc aattatttca taacctggca ttgcttcttc
     7141 ataaactgtt gccgcatctg cgttaaaagc tcctggattt cctgtgttaa aaggtataaa
     7201 aactttattg tttagaatta aagaatttgt gtaagcattt ctgtccatac cccaatttgt
     7261 gcggaaaact ttgtatttgt taccatagct agtagtttgg tttgcccaat aatctgccat
     7321 agcttcaaat tctgcatatc ttgagtcgct tgcaggaaca cgagtgataa gaattttgtc
     7381 aactgccaaa aattttcccc agcaatctat atgttctata taatcattta gcgggtcatt
     7441 aactagatga taattttcaa ttccaatata atcttgtact agttggctta cttcttgttg
     7501 acttaagctt ggattttcgg tataaactaa atcgcaagat gcagcaatgc caagcccgtc
     7561 agtcatataa tttccaccag tgtgaactaa attcattccg aaaacttcca tattccaata
     7621 ctcgccaaga acaataggaa gattgtcatc atttgttctg tctggacgat tataaggaaa
     7681 gtcaactatg ccaactttat tattttctcc atattcaata aaaatgggac taaaatctct
     7741 tgtccattga gagtcaatag gagcatttac aaaacttaca ttttccatgt ttactccatt
     7801 attttggagc tgagatgaag catatgattg agcaaaagat gaacaaacta tataaacctt
     7861 tacatattgc gatagttgtg aaattaagga atatgggaca gcaaaattat ttggaaaagc
     7921 aattataact ccttgatttg gttcaaattc agcaatactt cttgtgccac caacaggagg
     7981 ggcagtgtag ctaaaagcac gatttctgta gcttgttttg aactcttctg gcgagttcat
     8041 gtggtattta ctacgttcag cttcagtaaa ttctctttga gcaaatagcc ctaaatttag
     8101 aaaaatgaaa aaaactgaaa atataatctt attcataatt gagtttttat ttatttataa
     8161 tcaatcttga atttaaagtt tcttgatttg tgcttactgt aacaatataa actccatttg
     8221 taaagttttg agtgttaatc aaaatattgt tttcaccaga ataaatattt ataggattag
     8281 caaactcata cacaatttgt ccacttaaat ttaaaattct tacatttaca ctagaatttt
     8341 ctttcatgtt taaattgaga tatgcaatgt cttgagctgg atttggataa atttcaattg
     8401 agttttctaa aatgttggat tttgtattca ttactgccac aacattttga tgaggaggta
     8461 attttatatt atccaaccaa gcacaatctt gtccttgatt ataataagca tcttttatgt
     8521 atgaccattt gaattcgtgt tgaccagcgc taacattata tgtttctttt gaccaagcta
     8581 cttcgcctgc ccaagcacct tgttcagcat catcaatgaa gaaagcaaaa tggtcccaca
     8641 taaatcccca cataacaggt tcgcaactta cttttttata aaactcaatt tggtcatcgt
     8701 ttagaacttg taaatttata attaatgaag actctccacc acctgtagga gttccagatt
     8761 tgagtgcata ttcaccttcg taaacttcat ctgtaactat agtccaaggg aagttagaat
     8821 tattttccca attaaagcta gtcatagtat tagattccca gtcttcaatt tgttcgccta
     8881 cgctaaaacc taaacttaaa ctatctaaat acattccagc aattaattta aaatcaaaat
     8941 aaatagtagt gccacttgga atttcttctg ctaagctaat atcgaattca gttattacat
     9001 cttggttttc ttctaaggaa gaaatattat aaatagaatt attaatgcta ctaacatatt
     9061 cgctttcact actaatgctt actgttccag caacagaact agcgtgtcca atatttttag
     9121 cttgaatttt tattttaaaa gtttctccag tatcaagtga accattatta tttccaaatt
     9181 cactgtcgtc aatagcgtta aatgctaatt tcatttttgg agcattaaca ggaataaaaa
     9241 cattagatgt ccattcattt gcattgccat cagtgattgt aagaatagtt ttaatattag
     9301 tttggtcggc tattccatca actattgata atttaaaagc attattttca gtttttgttt
     9361 catcagcatt tatttgtcca aaactttttt gtccttctaa aattgttcca aggttttcat
     9421 ctactatgct taaatttgca acaacagcat ttgcaacatc aactcccacg ttttttaaag
     9481 tttcgttaat ataaatagtt tcattataat ctgctaaacc gttgttgttg ccttcttcat
     9541 cattaataga atactctgta tggataacat aaggacctga tggaacaatt actaaaattt
     9601 cttcttgata agtaatttta ttaaaagctg ttattgttag taaaactact ccaggttcgg
     9661 taagactttc aaaatttatt gttgcaactc cttgatttac ataacctgtg ccaagaattg
     9721 ttgttacgtc ttcgttaaat tttgtaatag aaactaaagc attttcttcg tcgcagttta
     9781 cctgaaatga agtttctcca attgtcataa cagcattatg gctaatgttc attgcttgag
     9841 gagtttttgt tctaaccatt aaagatgggt cgccaaaaat tgtccaagtg tctgccatag
     9901 attgcccttc gccacctgct tcaatcattt tgaacattcc attaaacgac aaaccaccaa
     9961 aagttctttt tatatttgaa gcatatgttt ccattaaaat gtcaatcatt tcgtcttgtg
    10021 cagtcatagg ttctgtccac gattggttga ttgtggaacc caaaaaagca atagctccag
    10081 ttggttgatt gtttttttga gcccttagcc aagcttcagc aaaacaggtt cttcctttaa
    10141 aatgcccatt aacacaagca acactccaaa taaaaggtaa tttattttca tttgttagag
    10201 cattaacatg tgaatttgta aattcagtat ttacccaaag agtttcgtca ccatgtccaa
    10261 tatagtttgc aattccagtt ccagcgttaa aatggttgct aatttgagta ttattccctc
    10321 cctgttgatt tacagatgta attgtgtaac catagttttg aagtttattt ttaataatat
    10381 tcatatgttg aacatcagat tctccattat gcccttgacc agcaccttcg cttgacgcag
    10441 atgtaaaacc atttgttaac caagtgtcag aagcgtttaa gtctctttca taagtaacag
    10501 tcctttgaac ttgagtttct atatctacaa gagaatttcc cgaaaaacga ccaataaaaa
    10561 tatctgcata tttatcatta ccagctaaat atgtgtagcc attgtctgta taatttgatc
    10621 caacattaat agtaggaata tcttcagcat caccaactaa taatacataa gttaaatcgt
    10681 tattgttgta ataattttga ataaaatttt ttaaatcatt tgagttagta cctattgtgc
    10741 taattaaaac catttcagtt tctatacctt tttcaagttt ccatttaata taaggagtca
    10801 ttgccgaagc ataagcgtct tttgcaataa ttaaaatctt accgggagtg ccctcttcaa
    10861 taggtgtata ttttgtagat gatttaaaat ttacaaaatg acgttcatat aatttgctga
    10921 actcataaga tacttttgaa ttgttattta caatttgatt tggaaaatta tcgtttgtaa
    10981 aagtaatttt taaaataatt tcactataaa ttcttaactc tttttgtata gcattgtaag
    11041 tgaaagggaa aaagctaata tttactcctc ttgcatcacg tacaataaat ggttcggaaa
    11101 taaaagcaga attttgtgga aaaaaatcgt caacaaaata ttctctacca tattcatagg
    11161 gaactgtttc agggtcttgg tttctataaa ttgttccttt agatggtgct atttcaatat
    11221 tttcaactgt aacatattcc gaagaaataa tattataaga aaaccctcct tggtttggaa
    11281 tcattattga agttggaata attggtaaat ctggacttcc ttttggaaaa ttgttccttg
    11341 cttttggaga tcttactatt actgattctc ctcgaggagt gttaacatct ttaaaataat
    11401 aagaattatt tgttaatttt aaagttaaac cttgcgaatt ttgctctaaa acatttatgc
    11461 tgaaattttc agatagctga gagtctagct taactaaatt tacttgagaa aataatgtta
    11521 aactcaagaa aaataaggta aatgataaga taatttgttt cataaaaata aaattaaatc
    11581 atattaattg tacaaattta atacattttt attctacaaa tatataccat atataaataa
    11641 tgtgttaaat aaaataaaaa attatacaat taggtgtttg aaagctatat taatgagttt
    11701 tcaaggattt tgtattaatt gtactatttc tgtttttatt gttgtagttt tttttaaaaa
    11761 gtgtaagtaa attatttaat tctttataac aagcagataa ttagattgtt aacttgaggt
    11821 gatttatgta aacataaaaa aataaaaagt tttttaaaat attttcaaaa aataatttgt
    11881 tttattcaga attgtattat ttttgcagtc ccaaaacaat ggggaagttc tttaaattaa
    11941 attttacaaa ataggcttac aataagttta tggtttaaat aaactttgat atgtaggcaa
    12001 acagaaatat cggggtgtag cgcagtccgg ctagcgcacc tgctttggga gcagggggtc
    12061 gcaggttcga atcctgccac cccgacaata aaaaagcttg acaatattgt caggcttttt
    12121 ttattattta ataatttttt tacatatttt gtaataataa aaagcaggac taacaaatga
    12181 ttaatgctag aattataatg ttttttcttg gatcactttt gatagccgaa agtgttttca
    12241 tgcttatagc tcttgctgta tctgttattt attctggtat agactttaat tatttgttga
    12301 tttctgctac agcaacatgt cttgcaggct tattgttagt acttccaaat cttaaaattg
    12361 ataaaaaagt tgaaaaaaga gaatcttatt taatagtaag tttagcttgg gtattgatgt
    12421 ctttatttgg tgcattgcca ttttttattg gtggatttat tccaagtttt actgatgctt
    12481 tttttgaaac aatgtcgggt tttactacta caggcgcgtc aattttaaat aatgtagaag
    12541 ctttgcccca tggacttttg ttttggcgta gtttgacaca ctggattggt ggaatgggaa
    12601 taatagtgtt atcaatagca gttatgccta tggtaaaatc tggaggtgtg cagttgtttg
    12661 tagctgaagc accaggaatt tcaccagata aacttcatcc tcgaattaaa gaaactgcaa
    12721 aaaaactttg gttggtttat gtgtcgttaa cagttgtgct gacttgtttg ctacatgttt
    12781 ttggaatgaa ttggttcgac tcgataaatc atgcattttc aacaatggcg acaggtggtt
    12841 tttctacaaa acaagctagt atagcacact ggacatcgcc atcaatacat tatataatat
    12901 tattgtttat gatactttca ggtataaact atagtatgtt gtattttgca tttacaggta
    12961 aaatcaaaaa acttattaaa aatactgaag ttaaatttta tttgttttta atcattggtt
    13021 ttggcttatt agttggtctt ggactttgga tttatatgga tttgcctctt gaaaaagctt
    13081 ttagagatgg aatgtttcaa gtagtgtctg ttatgacaac aacaggtttt actacaaatg
    13141 attatatgca gtggcatcca attggtttgt ggttgatatt attattagtt atgctaattg
    13201 gagggtctgc aggatcaact agtggtggaa ttaaagtcgt tagaatacat attctgttga
    13261 gaaatactgc tctcgaattt aagagattgt tgtttccaag tgccgtgtta cccgtaaaat
    13321 ataataaaac tgttttgtca gataaaacag taaatagtgt tttggcattt gtaatattat
    13381 atgttgtgtt aataatatta ggaacatttg ttttgtcgtt taccggctta gattttacaa
    13441 gctctcttgg agctgttgtt tcaagtattg gtaatgtcgg aacttcttta ggcgaaacag
    13501 gacctgctgt aggctttgca ggagttagtg cttttggaaa gtgggtttta tctttcttaa
    13561 tgttagtagg aaggctagaa ctctttacgg tgatgatttt atttacaaaa tcattctgga
    13621 aaaaataaaa atcagagatt aaaattcagg ctctacgctt ccttgtctga taatttctgg
    13681 ttcactacct gtgcaatcta taattgtaga tgctataagt ccaccataac cgccgtcaat
    13741 aactgcatca acaatatttt catattcttc gtaaatcaat tcagggtcgg tattatattc
    13801 aataatttca tcgtttgttc ttaacgaagt agttaaaatt ggattaccca aagcttttac
    13861 aatttctcta ataatattat tgtttggaac tctaattcct attgatttac gatttgcttt
    13921 caagattttt ggaacattgt tgttggcttc taatataaag gtaaaaggac ctggcaaatt
    13981 tcttttcatt aatttgaaaa tattgttgct caaaggtttt gtaaaatcgg aaatatgact
    14041 taagtcgtaa caaataaatg aaaaatttgc tttttcaggt ttaatacctt taattaatgc
    14101 aactctttca acagcttttg gtttggttat atcgcaacca ataccataaa cagtatcagt
    14161 aggataaatt attaccccgt cgtctttaag aatttcaact attcgattaa tatgcctatc
    14221 tgccggatta tcgggatgca tacggaaaaa ttcagccata ttatttattt aatttattat
    14281 agtcttttaa aaattgttct aaaccaatat ctgttaaagg atgttttaat aaacctaaaa
    14341 tagcacttag tggacaagtc gcaacatcag cacccatttc taaacatttg ataatgtgca
    14401 tagtgtgacg gatcgaagcc gctaaaactt gagtaccaaa ttcataataa ttatacacat
    14461 caacaatttg accaataagt tcaataccat ctgtgctgtt atcgtcaagg cgaccaataa
    14521 aaggcgaaac ataagtagca ccagcttttg ctgctaaaag agcttgacca acagaaaata
    14581 ctaaagtaca gtttgtgcga attcctttgt cagaaaaata ttttatagcc ttaacaccat
    14641 ctttaataat aggtactttt accacaattt gttgatgcaa agctgctaat tcttcgcctt
    14701 ctttaataat tccttcgtaa tcagtgctaa taacttctgc tgaaacatca ccatctacaa
    14761 ttttacaaat ttctaaataa tggtttaaaa tgttttgctt tccagaaatt ccttctttcg
    14821 ccattaggga aggattggtt gttacaccat ctaaaacgcc aagagcttgt gcttctttta
    14881 tttggtctaa atttgctgtg tcaataaaaa atttcatatg tctattttta taatttttgc
    14941 aaaattactt tataaaattc tatttacata acttatttga aagctttaat aaacaaattc
    15001 ttaaaaattg tcaacaatat caatgagaaa gggaaaaatc atgcaataaa cgctatgaca
    15061 gcaacgttta ttaattgcaa ggttaattta acagtttcat gttaataaaa accttgaaat
    15121 aaatggttta aaagtaaaaa tgtgtataat ttcgtgaaaa aaataagaat atcaataact
    15181 aaaatttaaa ttatgttatt tttacaagta gatagtttgc aaaatgtggc aagcgaggta
    15241 gctagcgaag aaactttatc actttttgaa ttagcgacta aaggtggtgt gataatgata
    15301 atattaggaa tgttgtcaat aattgcagtt tatttattta tagatagatt tattgctatt
    15361 caaaaagctc aaaaagatca aaaggctttt atgaacaaca ttaagaacaa tatctttgca
    15421 ggaaaagttg acactgcctt ggatttgtgt agagttcaag aaggtccttt ggcaagaatg
    15481 attgaaaaag gtattagccg aatcggtcgt cctttaagtg atattaatac tactgtggaa
    15541 aatgttggaa aacaggaagt ctcaaaattg gaaaaaggct taccactctt agcaacaatt
    15601 gctgggggag cgccaatgtt aggtttcctt ggaactgtaa caggtatggt gtcagctttt
    15661 tatgcaatgg ctaatgcagg taataattta gagataggta ctttgtcgag cggtatatat
    15721 gaagccttgg ttacaacagt aggaggactt atagtaggta ttttagcgta ttttgcttat
    15781 aatattttag ttgcaaaaat tactagtctt gttgttcaac ttgaagcaaa gactactgaa
    15841 tttatggata ttctaaacga acctgtaaaa taaaaaaata tggctataaa aaccagaagt
    15901 aagataagtt cggaaaccag tatgtcatca atgtctgact tggttttctt attgcttatc
    15961 ttttttatga taacttctac catgattgca ccaaatgcat tgaaactatt acttccatca
    16021 agtaatagcc aaactcaagc aaagccgttt acaacagtat ctattaaaga tatgaatgac
    16081 ggaacttata catttgcggt tgggatgcaa atatgtgccg aaacagatgt ggagcaacta
    16141 ttaaagaaag aacttatagg gcaagaagac ccttatgttt ctttacatgt tgataaatct
    16201 gttccaatgg agcaagtggt aaaagttatg aatattgcaa aaaataataa ttggaaatta
    16261 attttggcaa cacaaccaga ataaaaaatg agtctaaaaa agcataacat atatggaatt
    16321 atagcagctg ttatttatac aataacagtt ataattttgc tttttttatt aggctttact
    16381 acaccattgc ctcttccccc cgaagaggct atactgattg attttggggg cggtaacatc
    16441 aacgctggtg cttcatcgtc cacaaatact tcaaacgcac aaaacacctc ttctcaaatc
    16501 tcttcacacg atggcgtgaa cactcaagcg aatgaagaag ccccctttat gcaatcagga
    16561 actaatccag ccgaaaatcc taaaaaaaat gaagacgaaa ctgaaaccca agaaactaca
    16621 gctccaacaa atccaaatgc ggatagatta aataatctat tcggaggtgg agtttttgga
    16681 ggtggcacag gaagtggtac aggtggaact ggaactggag gtggaaatcc aggattagga
    16741 actggtgggt caggaactgg aagcggacca ggtggaatag gaggaggaat aggaaataga
    16801 agaatcttga aaaaagttga gcctgttgga aaagataata tggtaggaaa agttgtgcta
    16861 aaaattattg ttaacgacaa aggaaacgtt actagtgtaa cacttgtgag tactaactgt
    16921 aatgaatgtg ttcaactagc gatagatgca gttaaacaat ggaaatatga agcaaaaccc
    16981 ggaactggag agcaaacagg taatgttact gtagaattta aacaggtata aaatttattt
    17041 tattaaacca agcatagctt caacacaggc ttcaacaccc aaccaaactt tataaatatc
    17101 atcagcaagc atatagcaag gatttgttac aataagattt tttgtatcaa ttgctacttc
    17161 gtgataatct ttattgatat gtttaccacc aacatgttct atgattttag cagttgcttt
    17221 gtctgtgcca attgtaactt ccgcaccaag tacctttgaa ataattacag gagcaataca
    17281 taaagctgct attggttttt tagcagaatg aaaatcttta acaacttttt ctgcctcagt
    17341 atcaacagta tagttttcac cttcataagc aaacgaagat aaattttttg cagcaccaaa
    17401 accaccgggt aaaataagtg catcaaaatc ttttgcttta agttctttta tatctttaat
    17461 attacctcta gctattcttg ccgattcaac caaaacattt ctagtttcat tactaacttc
    17521 tccagttaag tgattaatta catgatattg ggctttattg ggagcaaaac atatgtaatt
    17581 tacttgattt tttgataaag ctaataaagt taaggtggct tcgtgtattt cactgccatc
    17641 aaatctacca cttccagata atattactgc aatatttttc ataattttta gtttttgtac
    17701 aaagatataa aaaaagttga aaaaattgaa atattaaagc ttaataattt gagaataaat
    17761 aaatgatttt gtatttttgt tgattaataa aaacaaccta ttaatgtttt ttacgttata
    17821 aaacagaatg taacttttaa aattgtgatt aaaaaaagta tttttatttt aaatatttta
    17881 cttattccat ttttggggtt tgctcaaact catttaagta atgattttgc aagtccaaca
    17941 gagatgaatg ttgtcagcga atgtaatttc gaaacttata cttataacca taattttggc
    18001 tctgatatac cagagttaaa gtgtaatcga atgtatcctg gagttagctc ttattttaaa
    18061 ttttcaactt tagaaaattc aaatttaact ttaaaagttc aactcgaaac agaaaaatta
    18121 tttggcttag ctttttatac tttagaaaat ggaacatatg ttgaattgaa atgcgacact
    18181 tatagatcaa caacaggcga attaaagatt tatgaggaag aagaattatt aagtacagaa
    18241 at
//
LOCUS       DAOPNX010000049        17997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_3738_length_17997_cov_5.404470, whole genome shotgun sequence.
ACCESSION   DAOPNX010000049 DAOPNX010000000
VERSION     DAOPNX010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17997
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3738_length_17997_cov_5.404470"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..1552)
                     /locus_tag="PKN53_06750"
     CDS             complement(149..1552)
                     /locus_tag="PKN53_06750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD88683.1"
                     /translation="MKSKLKNLILLFAFSAFTCNTFAQTEWITELPVFGYAYSMLRTK
                     DSCYAVLKRPAYYNEDSTQYIYNLQLTKVNPESEIVWYKTVFSDTLSFTYLSFIQLSN
                     EDFFITGRCHWCPFYAIVSKDGELKNFNRLDYYLNIPDSYSCFWSPVEISNSEIWVLG
                     GFSNLYITKINLNSNEIIVDSTAFCLQNVGSGFMNHLSNNNILITTGSKTYIIDTNQN
                     IINHKEFNFKFSGIRYKPFHINHFDSLYYFYGQTLDPYPNERYGFLVLNQQLDSVKFI
                     DNFDFYPNQSQVEYPRILDFDFPKPDNITMTGYIYWYYYQSYELPFILSYNMAKDSVE
                     YFHAYQGSVTRYSINLLKEDNSYVMLHAFQGNPGFVFLLKEKTDTSAQEVTKIYANNI
                     IEVYPNPVSDILTISCKNTVFSEILIIDSKGQQVKQIFIDSSAETAINVQDLAIGEYS
                     IIIETKEGLYIVKIIKI"
     gene            complement(1805..2359)
                     /locus_tag="PKN53_06755"
     CDS             complement(1805..2359)
                     /locus_tag="PKN53_06755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD88684.1"
                     /translation="MFSNVPIHSYKVVYLSGNYCFAGEIGSDSYSLQHGYGNFFDYFG
                     IWSDIFELSAIPELQTSCMCYYDGSVTINRATFSIYNDTIIYYKDSCRNYYNKFNNIS
                     SHTKENLFKVYPNPFTDFIHIDLPASADNCEIRVFDLMGKLIYSNKTHSNNTPLNLSQ
                     LVSGTYLIQVQTKQNIFTKRIIKN"
     gene            complement(2478..2855)
                     /locus_tag="PKN53_06760"
     CDS             complement(2478..2855)
                     /locus_tag="PKN53_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88685.1"
                     /translation="MKYLVKFSLLLSLIYLCKAGFPQQGTKNWEWEWAPIGAVWCYET
                     ANMGATDSYAQYWYVRSVSDTVYKSVNCRKLEVIRHFYPDFEPVKMQDRFTYQDGGDI
                     YFTTLTKESLCFLSATICNMATQ"
     gene            complement(3064..5520)
                     /locus_tag="PKN53_06765"
     CDS             complement(3064..5520)
                     /locus_tag="PKN53_06765"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HOD88686.1"
                     /translation="MQNWKLENIAKIVGGKFLGNKNNTFSQITTDSRNVTNYKDVLFF
                     SIKGQNNNGHKFISELYQKGVRNFVICENVDIEKFKEAAFLKVENSISALQKLAEYKR
                     SLFKNPIISITGSNGKTIVKEWLYHILNPFYTISRSPKSYNSQIGVPISLWLIDNNSD
                     LAIIEAGISLPGEMQALEKIIKPEIAVINNIGKAHSANFKSTEQLYQEKAILLKNCKT
                     TIVNKDDELLFNTVSKLNIPNTYTYSKNKNSDFFVEKIEKSSDNSLITIIFKDKKYKI
                     KIPFTDLGSIQNAITCFGIISIFDENKINQFIPRFESLHGIEMRLETIEGIQNSIIIN
                     DAYNSDINSLIIALDYLNQKKADKNSVLIMSDILESGENEEVFYKQISQIIKEKELKH
                     FIGIGSKLSKYKNLFPKDSSFHLDTNEFLEEISLHTFSNKAILLKGARSFKFEKIFNR
                     LQYKNHSSVIEIDLNSMKHNLNYFKSIIGTETKLMVMVKAFSYGSGYREVANFLQHNR
                     VDYLAVAFADEGVELRNLGIYLPIMVMNPNVKGVHNMIEHKLEPEIYSLNILEEFINE
                     IELTNIEEFKIHIKFDTGMHRLGFMKNDIPKLCKLINSCKKIKIASVFTHLSGSDSKD
                     LDYFTQQQVKVFDENYSLICKEIGYQPKRHILNSSGILRFPQYKYEMVRLGIGLYGLL
                     PDICDELIPVAVFKSEISQIHKINADETVSYNRSGKVKRDSIIATVPIGYADGFDRRL
                     GNGNWEFIVRGKKAKTIGNVCMDMCMIDITDIEANEGDEVIIFSSENSVIKMAEKLNT
                     IPYEIITGISQRVKRVYYEE"
     gene            complement(5524..6102)
                     /locus_tag="PKN53_06770"
     CDS             complement(5524..6102)
                     /locus_tag="PKN53_06770"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOD88687.1"
                     /translation="MFLEQPKKGEPKKGWIEVIAGSMFSGKTEELIRRLRRAEFAKQK
                     VEIFKPAIDTRYDAEDVVSHNATSIRSTPVTSSQNILLLTGEVDVVGIDEAQFFDNGI
                     IEVCIKLADQGIRVVVAGLDMDFLGRPFGPMPALFAVAEYVTKVHAVCVHCGDLAQFS
                     YRQSEDNAVVVLGEKDIYKPLCRRCYNEETKK"
     gene            6166..6954
                     /locus_tag="PKN53_06775"
     CDS             6166..6954
                     /locus_tag="PKN53_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88688.1"
                     /translation="MEKIFIVKEKLNKILDFINSINNVAITELEKEILLQKLKDVYFE
                     LSIIEVTKIEEEKDSKVIEQPVEQKVVKPEVVAEKVEEKPIVNKEPEKTIVEEKPVVE
                     TKKDSFVPIDFFDLDEEESPEPKDVVFEKKEEVKKTQSVEQGNLFSSDNNSSSPKYIG
                     EHLGQNKTSLNEALASQTSVQDLNARIKPVSDIRSAMSLGDRFLFVRELFNGSTDLFD
                     ETIANLNSLNSFDEAQSYMKSHFQWDIADSNVENFMNIVRRRYV"
     gene            6932..8233
                     /locus_tag="PKN53_06780"
     CDS             6932..8233
                     /locus_tag="PKN53_06780"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HOD88689.1"
                     /translation="MLEEDMYRVKIFLGILSFVFSFSSNLHAQNLENTKKRIEILSSK
                     KFAGRGYVGKAGDLSANFIADELSKMGLLKFGDSYFQEFSYPVNTFPGKLLVKIDNKQ
                     LIPGVDYLIGPASPKVKQEYELWKPDSVLLNDTIGIIKHIGKNIALKSSVFVIDYAQT
                     ENIDIKKFYISNFVMGNSFFAGIVELIPEELMWSVSRRQQSYPLVKIKREFFDPNSKT
                     IFIDVKPALNPNFKAKNVIAYIPGETDDFVVFTAHYDHLGKMGKKVYMPGAQDNASGV
                     ALLLDLADYYSKNKPKYSIAFMFFFGEEAGLLGSLYYVLNPIFELEKIKVLLNIDLAG
                     TGDEGITIVNAANEKYEKEWNLFQKLNSENNFFEIMKSRDISANSDHYPFHEMGVRSL
                     FIYTMGGKTYYHHPKDKLETLTFGGYYQLFDLITKFVENYE"
     gene            8226..8915
                     /gene="rsmI"
                     /locus_tag="PKN53_06785"
     CDS             8226..8915
                     /gene="rsmI"
                     /locus_tag="PKN53_06785"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOD88690.1"
                     /translation="MSKLFVVPTPIGNLEDMTFRAIRILKEVDFILAEDTRTSGFLLK
                     HFEINTKMYSFHKFNEHKILDNVVERILTSNSVALISDAGTPGISDPGYLLVKACLEN
                     EIDVECLPGATAFVPALVNSGLPNDRFCFEGFLPQKKGRNKKMQSLVEEERTMVFYES
                     PYRLIKTLELFVEFFGENRRASVSREISKLHEENVRGNLKELITHFSEKTIKGEIVIV
                     VEGKTKQKEIE"
     gene            complement(8988..12986)
                     /locus_tag="PKN53_06790"
     CDS             complement(8988..12986)
                     /locus_tag="PKN53_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOD88691.1"
                     /translation="MKKILFLSILFFSCFVFQVQSQVTVTVGTGTEEGRYNPIYTFYG
                     YNYTQQIYTSAELNTAGAVAGMQINAIRFYWTGAGTLNNADVWTVYIGETTQSSFTST
                     TNWVPFASLTEVYTGTITLPATAGWFTITLTTPVVWNGGNIVVAIDENVPGYGPSTTR
                     WQATITTSNTAIYYYNDNTNPDPSTPPSGTTTTTRPNIQFEMMSLTPCAGNPDPGTAT
                     PASQLVPIGGSANLTLSGSTVAAGLSYQWQRATSPTGPFTDITGATSLSYNTGALTTS
                     YYYRCIVTCVATGDADTSTIAFVEVVQSYNMSNTTITTCDALFYDSGGPTGTYSANQN
                     LTLTIMPDIVGGNVIVTFNSFTLENNYDKLFIYNGNSITAPLIGQYTGSTSPGTVTST
                     AADGSLTFRFTSDGSVQYAGWEAVISCSDPCNYVYETPTFDQVGPFCSGWTIPDLPTT
                     SNNGYEGTWAPAIDNTATTTYTFTPNEGECATPTTMTIVVNPNNPASFTQYSSYCRGD
                     SIPELPTISNDGIEGTWSPAINNWNTTEYTFVTNDGTCAWTTMEIEIDTVPAEIINNS
                     GSNELNCDLTAIYLTAEGGVSYSWDGVYFNTFQGKTITDPGTYTVIVRDANSCTGEAS
                     IEITQNLNPPVATITNNTGSDYLNCHINEISLTASGGATYTWNGGSSPNSATNTFNTE
                     GTYYVTVTADNGCDTIIYYMITDETILDITENITSPSCFGMTGSAEIIINSGGQAPFI
                     IEWTDGVTTFSRTNIPTNTPFGYIITDADNCSTIRSLTITTPEQIQVNFVSDSVSCYG
                     GHDGSLDISNSTGGVPPYSYQWSVAGGTGSQITGLAAGTYFVTISDAKNCTASGSHSV
                     GQPEQISIDMQITDAICGQTGGAINAEATGGVGGFTYEWSTSSISNNITGLTPNMYTL
                     TVTDANNCTQTASATVDKTGDIIANITVVQPILCGGNPDAILQGSSANGAMPWTFNWS
                     NGATTEIINNLSTGTYILTVTDAWGCTGSTEETLEAAIDVSINSNVSHVKCYGNNDGK
                     INISIISGEPPFTIAWSNDSTELNQSSLPAGLYSVTITDNRDCAITENFEIIEPPVLE
                     LAFTKKDVSCYSDLNGTITMNVTGGTEPYTYSITTPRNQVVTNTASANNLSIGKYIMK
                     VVDNNGCTDIKETNIYQPAKLSAKVNVTNPSCMGNDDGSIIVTPFGGTSPYRYTYDNF
                     DLDTNIIVNAKEGRYSIEVEDANSCKLILSDIYVGDGLLPCLKIPNAFTPNGDGINET
                     WIIENIDLYPDAQIFVYTRWGEQIYYGTPKSDPWNGTYNGKKMPTGAYLYIVETRNND
                     AGVYKGTVTIVY"
     gene            complement(13330..14178)
                     /locus_tag="PKN53_06795"
     CDS             complement(13330..14178)
                     /locus_tag="PKN53_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88692.1"
                     /translation="MYLFADSGSTKTHWVITDFEYNILTEFKTIGLNPYFTSEQKLLA
                     TLTEAFPQKFDSLKIEKLYFYGSGCSIEENYYHFRQTFLDFFFNAEISIFSDMLGAAR
                     ASLKTDEGICAILGTGVNSCFYNGQAIKQNAISLGYILGDEGSGAYIGKLFAKFYLEK
                     RLDPELSDKFYFETGENKASILNAVYKNPNPNKYLANFCHFIYKNIEHKQLTELVEFS
                     FNKFFKNYIVIFNDYKNKKLSLTGSLANAFKDILIKTAANFGTQQPIFIDNPIYGIID
                     YHKITN"
     gene            complement(14236..15234)
                     /locus_tag="PKN53_06800"
     CDS             complement(14236..15234)
                     /locus_tag="PKN53_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOD88693.1"
                     /translation="MATSYKELGLVNTKEMFEKATKGGYAIPAFNFNNMEQMQAIVAA
                     CAETKSPVILQVSSGARKYANQTLLRYMAQGAVEYAKELGWENPQIALHLDHGDTFEL
                     CKSCIEMGFSSVMIDGSHLQYEENVALTRKVVEYAHKFDVSVEGELGVLAGVEDDVSA
                     EHSTYTKPEEVVDFVSRTGVDSLAISIGTSHGANKFTPEQCTRDANGKLVPPPLRFDI
                     LKEIEKQIPGFPIVLHGSSSVPEEYVKTINENGGRLKDAIGIPEDQLREAAKSSVCKI
                     NIDSDGRLAMTATIRKFFNDSPDKFDPRQYLGPAREELKKLYIHKIKNVLGSFEKA"
     gene            15639..16223
                     /locus_tag="PKN53_06805"
     CDS             15639..16223
                     /locus_tag="PKN53_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOD88694.1"
                     /translation="MLKNLDIFNIISNDLEPATGRLLISEPLLSDDIFSRSVILIAGM
                     ENKSAFGFILNNPSNRNLHELIDGVEIENLPVFIGGPVEKDVLFCIHTIDYLPDSIHI
                     NDGIYLSGNFDTIKTLVNLGIINNSNARFFIGNSGWAPEQLHDEINFNYWLVSETTRE
                     FVFSDFKNQWERALDFVDKKYEIWKKFPKNPEFN"
     gene            complement(16229..16555)
                     /locus_tag="PKN53_06810"
     CDS             complement(16229..16555)
                     /locus_tag="PKN53_06810"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOD88695.1"
                     /translation="MDTKNIKEKNNFLLKTLNFIIKHKYVVTLVFFALWVTFFDTYNL
                     IDRFKKLRKLNALKKEIKYYEDEISLYTRQYEELFSNKDNLEKFAREEYQMLEEDEDL
                     FIIIDD"
     gene            complement(16656..17243)
                     /locus_tag="PKN53_06815"
     CDS             complement(16656..17243)
                     /locus_tag="PKN53_06815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOD88696.1"
                     /translation="MKILLIYANYKEQSFTSAVRDTIAYTFHQKGHEVVIRDLYEIKF
                     NPVLSKKDLESIEKEIFPVDIMDEQKFIQWADFICFVYPIWWSGMPAILKGYIERVFV
                     QGYGFDFIDGEIIPKLKNKRIAIFNTTGLNNIYKDQASLDALNKMTEECILGFSGMKT
                     VVHKYFKSVRKANSKEKIKSLEEVKNAAEELLEML"
     gene            complement(17388..>17997)
                     /locus_tag="PKN53_06820"
     CDS             complement(17388..>17997)
                     /locus_tag="PKN53_06820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD88697.1"
                     /translation="VGGPSLMGNVYNTVMLKFYGHLMMNVYNGIAEFRNFVALDLPVA
                     FYENTFFIGGKNGEIANFDFNLILNEGFANDGVFTLPADIRYLDIKLQGTDKLLLAGY
                     DNTSGTDKAVLARFGISTNSSANIQSVSSSISIYPNPSNGIFQIESQVFSVEVFDNLG
                     RVILNQNNTNTINLQQFGSGVYYAKILTTANATTNTVKLVVY"
BASE COUNT         6069 a         2700 c         2783 g         6445 t
ORIGIN      
        1 atctttacca caaattaata caagttatca taaacaacct tgtgaaactt gacaactata
       61 gaacaagttt tgattttcat tctgacaaaa taaatttatg caagcaacac ttgcaataaa
      121 taacaataat aatttccttt tcataacttt aaattttaat aatttttact atatataaac
      181 cctcttttgt ttcaataata atagaatatt cacctatagc taaatcttgt acgtttatag
      241 cagtttcagc acttgaatca ataaatattt gtttaacttg ctgtcctttt gaatcgataa
      301 ttaaaatttc tgaaaaaaca gtatttttac agctaattgt taaaatatcg ctaacaggat
      361 ttggatatac ttctattata ttgttagcat atattttagt tacttcttga gcactggtgt
      421 cagtcttttc tttaagtaaa aaaacgaatc ctgggttccc ctgaaaagca tgaagcataa
      481 cataagaatt atcttctttt aaaagattga tactatatct ggttaccgat ccttgataag
      541 catgaaaata ctcaacagaa tctttcgcca tattataact aagtataaat ggcagttcat
      601 acgattgata ataataccaa taaatataac ctgtcattgt aatattatct ggctttggaa
      661 aatcaaaatc cagtattctc ggatattcaa cctgcgactg attagggtaa aaatcaaaat
      721 tatcaataaa ttttacagaa tcaagttgtt gatttagcac caaaaaacca tatctttcat
      781 ttggatatgg atcaagtgtt tgtccataaa aatagtaaag actgtcaaaa tggttaatat
      841 gaaatggttt atatctaatt ccactaaact taaaattaaa ttccttatga ttaataatgt
      901 tttgattagt gtcaattata tatgttttag atccagtagt tattaatata ttattgttcg
      961 acaaatggtt cataaacccc gaacccacat tttgcaagca aaaagctgtt gagtccacaa
     1021 ttatttcatt gctattaagg ttgatttttg tgatatacaa atttgagaat ccacctaaaa
     1081 cccatatttc tgaatttgat atttcaacag gcgaccaaaa acaactataa ctatcaggaa
     1141 tattcaaata atagtcaagt ctgttgaaat tttttaattc accatcttta gatacaatag
     1201 cgtaaaaagg acaccaatga catctacctg taataaagaa atcttcgttt gaaagctgaa
     1261 tgaaactaag atatgtaaaa ctaagtgtgt cggagaaaac agttttatac cacactattt
     1321 cgctttctgg atttactttg gtaagctgta ggttgtatat gtactgtgtt gaatcttcgt
     1381 tataatatgc aggccttttt agtactgcat aacaactgtc ttttgttcta agcattgagt
     1441 aagcatatcc aaaaacaggt agttctgtaa tccattcagt ttgcgcaaat gtattacatg
     1501 taaatgcact aaaagcaaat aataagataa gattctttaa tttacttttc ataacttaaa
     1561 ttttaataat tttcactata ttcttcttta attatagaat actctcctgt agcaaaatcc
     1621 tgtcctatat attctaaact atctttttac aaagataaca aaatttttta tacttacagt
     1681 tgaggaaagc aagtttttat acaaatgtgc gaatattggg tgaataagtg ttaaaaactt
     1741 tgattaaagt gccgattgta atttttattt atttttaaaa ttgatatgaa gtcaaatgga
     1801 ttttttaatt tttaattata cgtttggtaa atatattctg ttttgtttgt acttgtatta
     1861 gatatgttcc tgaaacaagt tggcttaaat ttaatggagt gttgttacta tgtgttttat
     1921 ttgaatatat tagttttccc ataaggtcga atactctaat ttcgcaatta tctgcagaag
     1981 caggaaggtc aatatggata aaatctgtaa aagggttggg gtaaacttta aacaaatttt
     2041 ctttagtatg cgatgaaatg ttattaaact tattgtaata gtttcggcaa gagtctttgt
     2101 aatagattat ggtatcattg taaatgctga acgttgctct attgattgtt acactaccat
     2161 cataataaca catgcaacta gtttgcaact caggaatagc tgataactca aaaatatctg
     2221 accatattcc aaaataatca aaaaaattac cataaccatg ctgaagactg taagaatcag
     2281 agccaatttc tcctgcaaaa caataattac cacttaagta aactacttta tacgaatgaa
     2341 taggaacatt actaaacata atgcattata aggagtaaat tccattcctg cgccggataa
     2401 gaaacctgct tcccaattga aaatagaatc aagataaaaa gaatttatac aattttcata
     2461 catgggcatt tgccatgtta ctgtgtcgcc atgttgcaaa tcgtagctga aagaaagcac
     2521 aaactctcct ttgtcagcgt tgtaaaatag atatcgccac catcttggta tgtaaagcga
     2581 tcttgcattt ttacaggttc aaaatctgga taaaaatgtc gtattacttc taattttcgg
     2641 caatttactg acttatacac agtgtcgctt acagaacgca cataccaata ttgtgcatac
     2701 gaatctgttg cacccatatt tgctgtttca taacaccata ctgctccaat tggtgcccac
     2761 tcccattccc agtttttggt gccttgctgg ggaaaacctg ctttgcataa ataaatcagg
     2821 ctaagcagta atgaaaattt tactaagtat ttcataattt ttagttttta gtttaattat
     2881 atgaatactc tcctatgcca aaatcctatc ccaaatattc ttataactaa ttaatttcta
     2941 tttttacgaa aataacaaaa ttttttatac ttacagttga ggaaagcaag tttttatata
     3001 aatatgcgaa tgttgcgtga ttaaatgatg aaaaattcta actattatga agaatatctc
     3061 tttttattct tcataataaa ccctcttaac tctttgacta attccggtaa ttatttcgta
     3121 aggaattgta ttgagttttt ctgccatttt aataacagaa ttttctgagc taaaaattat
     3181 tacttcatcg ccttcgtttg cttcaatgtc agtgatgtca atcatgcaca tatccataca
     3241 tacattacca atggtttttg cttttttgcc tctaacaata aattcccaat ttccatttcc
     3301 aagtcttcta tcaaatccat cagcatagcc aattggaacg gtagcaataa ttgaatctct
     3361 ttttactttt cccgacctat tataactaac agtttcgtcg gcattaattt tgtgaatttg
     3421 tgaaatttca gatttaaaaa cagcaacagg aataagttca tcgcaaatgt ctggtaataa
     3481 gccatataag cctattccaa gtctaaccat ttcgtatttg tattgtggaa atcttaaaat
     3541 tcccgaagaa tttaaaatat gtcttttagg ctgataacca atttctttgc aaattaaaga
     3601 ataattttca tcaaacactt ttacttgttg ctgagtaaaa taatccaagt ctttactatc
     3661 gctaccagaa agatgagtaa aaacagaagc aattttaatt tttttacagg aattaattaa
     3721 tttgcaaagt tttggaatat catttttcat aaatcctaat ctatgcattc ctgtgtcaaa
     3781 ttttatatga attttaaatt cttcaatgtt tgtaagttca atttcattaa taaattcttc
     3841 taaaatattt agagaataaa tttcaggctc taatttatgc tctatcatat tgtgtacgcc
     3901 ctttacattt gggttcatta ccataattgg aagataaatc ccaagatttc ttaattcaac
     3961 accttcgtca gcaaaggcta ctgctaaata gtcaactctg ttgtgttgca agaaattagc
     4021 aacttctctg tagccacttc cgtaagaaaa agctttaacc ataaccatta actttgtttc
     4081 tgtaccaatt attgatttga aatagttaag attatgtttc attgagttta aatctatttc
     4141 tattacagag gagtgatttt tgtattgaag acgattaaag attttttcaa acttaaaact
     4201 acgtgctcct tttaacaaaa tagccttatt gctgaaagtg tgaagggaaa tttcttcaag
     4261 aaattcgttg gtatctaaat ggaaagaaga atctttagga aataaatttt tgtatttact
     4321 caatttactg ccaattccaa taaaatgctt gagttctttt tctttaataa tttgagaaat
     4381 ttgtttgtaa aaaacttctt cgttttcacc actttcaaga atgtccgaca ttattaatac
     4441 actgtttttg tcagcttttt tctgatttaa atagtcaaga gcaattatta aagaattaat
     4501 atcagaattg taggcatcat ttataataat gctattttga attccttcta tagtttccaa
     4561 tctcatttca attccatgaa gactttcaaa tcttggaata aattgattta ttttattttc
     4621 atcaaaaatt gaaataatac caaaacaagt aatagcattt tgaatagagc caaggtctgt
     4681 aaaaggaatt ttaattttat attttttgtc tttaaaaatt attgttataa gcgaattatc
     4741 acttgatttt tcaatttttt ctacaaaaaa atcagaattt ttatttttag aatatgtgta
     4801 agtattagga atatttaatt tggaaactgt gttaaaaagt agttcgtcat ctttgttgac
     4861 tatagtagtt ttacagtttt ttagcaatat tgctttttct tgataaagtt gttctgtaga
     4921 tttaaaattt gccgaatgag ctttacctat attattaata acagcaattt ctggtttaat
     4981 aattttttcg agagcttgca tttcgccagg taaggaaatt ccagcttcga ttattgctaa
     5041 atcgctgtta ttatctatta gccataagga aattggtaca ccgatttgag aattataact
     5101 ttttggagag cgacttatag tataaaaagg atttaaaata tgatatagcc attctttaac
     5161 aatagttttt ccattactac ctgttattga aattatagga tttttgaaaa gacttctttt
     5221 gtattcggca agtttttgaa gtgcagaaat tgagttttcg acttttaaaa aagcagcttc
     5281 tttaaatttt tcaatatcaa cattttcgca aattacaaag tttctaaccc ctttttgata
     5341 aagttcactt atgaatttat gtccattatt attttgacct tttattgaaa aaaacaaaac
     5401 atctttgtaa ttagtaacat ttctactgtc tgttgtgatt tgcgaaaatg tattgttttt
     5461 atttcctaag aattttccgc caacaatttt tgcaatattt tcgagtttcc agttttgcat
     5521 ttgttatttt tttgtttctt cgttgtaaca acgtctgcat agaggtttat aaatgtcttt
     5581 ttcgccaagt acaaccacgg cattgtcttc ggattgtctg tacgaaaact gagccaaatc
     5641 gccacaatgt acgcaaacag catgaacttt agtaacatat tcagcaacag caaataaagc
     5701 aggcattgga ccgaaaggtc gtcctaaaaa atccatatcc aaacccgcca caacaaccct
     5761 aatgccttgg tctgctaatt tgatgcaaac ttcaataatt ccattatcaa aaaattgggc
     5821 ttcatcaatt ccaacaacat caacttcgcc agttaataaa agaatgtttt gtgaagaagt
     5881 tacaggtgta gagcgaatag aagtagcatt gtgtgaaaca acatcttcgg catcatatct
     5941 cgtatcaata gcaggtttga aaatctcaac tttttgtttt gcaaattcag ccctacgcaa
     6001 acggcgaatt agttcttcgg ttttccccga aaacattgat ccagcaataa cttcaatcca
     6061 gccttttttt ggctcacctt ttttaggttg ttcaagaaac atttaacaat aatttttata
     6121 tttttgtaaa aataatttaa ttacaaattt agaaaaatat tttgtatgga aaagattttt
     6181 attgtaaaag aaaaattaaa taaaattctg gattttatta acagtataaa taatgttgct
     6241 attacagaat tagaaaaaga aattttatta caaaaactta aagatgttta ttttgagttg
     6301 agtataattg aagtaactaa aatagaagag gagaaagatt ctaaagtaat agaacaacct
     6361 gttgagcaaa aagtagttaa gccagaagtg gttgctgaaa aagtagaaga aaaacctatt
     6421 gtaaataaag agcctgaaaa aactattgta gaagaaaaac ctgttgttga aactaaaaaa
     6481 gatagttttg tccctattga ttttttcgat ttagacgaag aagaaagtcc agaacccaaa
     6541 gacgttgtgt ttgaaaagaa ggaagaagtt aaaaaaacac aaagtgttga gcaagggaat
     6601 ttattttcaa gtgataataa ttcaagttct cctaaatata taggcgagca tttgggacaa
     6661 aataaaactt cgttaaatga agctttagct tctcagacta gtgttcaaga tttgaatgca
     6721 aggataaaac ctgtttcaga tattcgctct gccatgagtt taggagatag atttttattt
     6781 gttcgtgaat tgtttaatgg aagtacagat ttatttgatg agacaatagc aaatttgaat
     6841 tctttaaatt cttttgatga ggctcaaagt tatatgaaat ctcattttca atgggatata
     6901 gcagattcta atgtggaaaa ctttatgaat attgttagaa gaagatatgt atagggtaaa
     6961 aatttttctt ggaatattaa gttttgtttt ttctttttct tcaaatttac atgctcaaaa
     7021 tcttgaaaat acaaaaaaaa gaattgaaat tttaagttct aagaaatttg ctggacgagg
     7081 atatgtggga aaagcaggcg acttgtctgc aaattttatt gcagatgaac tttcaaaaat
     7141 ggggttgcta aaatttggag attcttattt tcaggaattt tcttatcctg taaatacttt
     7201 tccaggaaaa ttacttgtaa aaatcgataa taaacaattg attcctggag ttgattattt
     7261 aattggtcca gctagtccaa aagttaagca agaatatgag ttgtggaagc cagattcggt
     7321 attattaaat gatactattg gaattattaa acatataggt aaaaatattg cattaaaatc
     7381 atctgttttt gtaattgact atgctcaaac agaaaatata gatattaaga aattttatat
     7441 ttcaaatttt gtaatgggca attctttttt tgctgggata gtagaattaa ttccagaaga
     7501 attaatgtgg tctgttagta gaaggcagca atcatatccg cttgtgaaaa tcaaaagaga
     7561 attttttgac ccaaattcca aaactatttt tattgatgtt aaacctgcac taaatcctaa
     7621 ttttaaagca aaaaatgtaa ttgcttatat tcctggcgaa acagatgatt ttgttgtttt
     7681 tactgcacat tacgaccatt tgggtaaaat gggtaaaaaa gtttatatgc ccggagctca
     7741 agataatgca agtggggtag ctttgttatt ggatttggca gattattatt ctaagaataa
     7801 accaaaatat tccatagctt ttatgttttt ctttggcgag gaagctggct tgttaggttc
     7861 gttgtattat gtattaaatc caatttttga attagaaaaa attaaagttt tactaaatat
     7921 tgatttagca ggaactggtg atgagggaat aactatagtg aatgcagcaa acgaaaaata
     7981 tgaaaaagaa tggaatttat ttcaaaagtt aaattctgaa aataattttt ttgaaataat
     8041 gaaatcacgt gatatttcag caaatagtga tcattatcct tttcacgaaa tgggagtgag
     8101 gtctcttttt atttatacta tgggtggaaa aacttattat catcatccta aagataaatt
     8161 agaaacatta acttttggtg gatattatca actttttgat ttaataacaa aatttgtaga
     8221 gaattatgag taaacttttt gttgttccta cacctatagg taatttagaa gatatgactt
     8281 ttagagccat aagaatttta aaagaagttg attttatttt ggctgaagat acacgcactt
     8341 cggggttttt gttaaaacac tttgaaataa atacaaaaat gtatagtttt cataagttta
     8401 atgaacataa aattttagat aatgtagtag aacgaatttt gacaagcaat tcagttgctt
     8461 taatttcaga tgctggaact cctggtattt cagaccctgg atatttgctt gttaaagctt
     8521 gtttggaaaa tgaaattgat gttgaatgtc ttccgggagc aactgctttt gtgccagctt
     8581 tagtaaattc tggtttaccc aatgatagat tttgctttga aggtttttta cctcagaaaa
     8641 aaggtagaaa taaaaaaatg cagagcttgg tagaggagga gagaaccatg gttttttatg
     8701 aatctcctta tagattgata aagactttgg aattgttcgt tgagtttttt ggcgaaaatc
     8761 gtagagcttc tgtctcgcgt gaaatttcaa aattacatga agaaaatgtt agaggaaatt
     8821 tgaaagaatt aattacgcat ttttcagaaa aaactataaa aggtgaaata gtaatagttg
     8881 ttgaaggaaa aactaagcaa aaagaaatag aataaaacat tgaggaatta aaaaaaagcc
     8941 aactaattta ttatctatta gttggctttt ttatttccta gtatttttta gtacactata
     9001 gttacagtgc ctttatatac gccagcatcg ttatttcttg tttctacaat gtacaagtat
     9061 gctcctgtag gcattttttt accattataa gttccattcc aaggatctga ttttggggtt
     9121 ccataatata tttgttctcc ccaacgggta tatacaaaaa tttgagcatc tgggtagaga
     9181 tcaatatttt caattatcca agtttcgtta ataccatcac catttggagt gaaagcattt
     9241 ggaattttta gacaaggcaa tagcccatct ccaacatata tatcgcttaa aataagttta
     9301 caagaattag cgtcttcaac ttcaatgcta tatctacctt cttttgcatt gacaataata
     9361 ttagtatcta aatcaaagtt atcgtatgtg tatctatatg gtgatgttcc accgaatgga
     9421 gtaactataa ttgagccatc atcattcccc atacaacttg gatttgtaac attaacttta
     9481 gcacttagtt tggctggttg ataaatgtta gtttctttaa tgtcagtaca accgttatta
     9541 tcaacaactt tcattatata ttttccaatt gataagttat ttgctgaagc tgtatttgtt
     9601 acgacttgat ttcttggagt tgttatagaa taagtgtaag gttctgttcc tcctgtaaca
     9661 ttcatagtta tagtgccgtt taaatcactg taacaactaa cgtctttttt tgtgaaagct
     9721 aattcaagaa ctggtggctc aatgatttca aaattttctg taatcgcaca gtctctatta
     9781 tcagtaattg taacagaata tagaccagca ggtaaagaac tttgattcaa ttctgtagaa
     9841 tcatttgacc atgcaatagt gaatggaggt tcaccactta tgatgctaat attaatttta
     9901 ccatcattgt taccataaca tttcacatga gatacattag aatttatact tacatctatt
     9961 gcagcttcaa gtgtttcttc tgtacttcca gtacaacccc aagcatcagt aactgtaagt
    10021 atgtatgtac ctgtagatag attattaata atttcagttg tagcaccatt gctccaatta
    10081 aaagtccatg gcatagctcc attagcagaa ctaccttgta aaattgcatc tggatttcct
    10141 ccgcacaaaa taggttgaac aactgttatg ttagcaataa tatctccagt tttatcgaca
    10201 gttgcactag ctgtttgggt gcaattgtta gcatctgtaa ctgttagtgt gtacatgttt
    10261 ggagttagtc ctgtgatatt gttagatatg ctactagttg accattcata tgtaaaacca
    10321 ccaacaccac cagtagcttc agcatttata gctccacctg tttgtccaca aatagcatca
    10381 gtaatttgca tgtcaattga tatttgttct ggctgaccta cagaatgact tcctgatgca
    10441 gtacagtttt tagcgtctga aattgtaaca aaatatgtac cagcagctag acctgttatt
    10501 tgacttccag ttccaccagc aacagaccat tggtaagaat atggaggaac acctccagtg
    10561 gagtttgaaa tatctaggga accgtcatga ccaccatagc aactgacact atcactaaca
    10621 aagtttactt gtatttgttc tggtgtggta atggttagcg atcttatagt tgagcagtta
    10681 tcagcgtcag taataatata tccgaatgga gtatttgtag gaatattggt acgagaaaat
    10741 gtagtaacac catctgtcca ttcaattatg aatggagctt gaccaccaga attaataatt
    10801 atttcagcac ttcctgtcat gccaaagcaa gaaggacttg tgatgttttc tgttatatct
    10861 aaaattgttt catctgttat catgtaatat atgatcgtgt cacatccgtt atcagcagta
    10921 acagtaacat aataagtgcc ttcagtatta aaagtatttg ttgcagaatt tggagaagaa
    10981 ccgccattcc aagtataagt agcaccaccc gaggcagtta aactaatttc atttatatga
    11041 caattaagat agtctgaacc agtattattt gtgatagttg caacaggagg attgagattt
    11101 tgagtaattt caatacttgc ttcgcctgta cagctattag catctcgcac aattacagta
    11161 taagttccag ggtctgtaat agtcttgcct tggaaagtat taaaataaac tccatcccat
    11221 gagtaactta cacctccttc agcagttaaa taaatagctg ttaaatcgca atttagttca
    11281 ttgcttcctg aattgtttat aatttcagca ggtacagtat caatttcgat ttccatagta
    11341 gtccaagcac aagttccgtc attagtaaca aaggtatatt cagttgtatt ccagttatta
    11401 atagcaggag accaagtgcc ttcaatgcca tcgttagaaa tcgttggtaa ttctggaatg
    11461 gaatctccgc gacaatatga agagtattgg gtaaagctag cagggttatt tggatttaca
    11521 acaatagtca ttgtggttgg ggtagcacat tctccttcgt taggtgtaaa agtatatgtt
    11581 gtagtagcag tattatcaat tgcaggagcc caagttcctt catatccatt gttagaagtc
    11641 gttggtaggt ctggaatagt ccaacctgaa cagaaaggac caacttggtc aaaagtagga
    11701 gtttcgtaaa cataattaca agggtctgaa catgatatta ctgcttccca accagcatat
    11761 tgtacacttc catctgatgt aaatctaaaa gtaagtgatc catctgcagc tgtagatgtt
    11821 acagttccag gtgatgtgct tcctgtatat tgaccaatta gaggggcagt aatagagttg
    11881 ccattataaa tgaataattt atcataatta ttttctaaag tgaaactatt aaaagtaaca
    11941 attacatttc ctcctacaat atctggcatt atggtaagtg taagattttg attagcagaa
    12001 taagttcctg taggacctcc tgaatcgtaa aatagtgcat cgcaggttgt tattgtagta
    12061 ttagacatat tatatgactg tacaacttca acaaaagcaa tagttgaggt atctgcatct
    12121 ccagttgcaa cacatgtaac tatacagcga taatagtatg aagttgttag cgctcctgta
    12181 ttatagctta gtgatgttgc gccagtaata tctgtaaaag gaccagtagg tgaagtagct
    12241 ctttgccatt gatacgataa gccagctgca acagtagaac cagataaagt taggttagca
    12301 ctacctccaa taggcactaa ttgtgaggca ggagtggctg tacccggatc tggattacca
    12361 gcacaaggag taagactcat catttcaaat tgtatgttag gacgagttgt tgttgttgta
    12421 ccagaaggtg gcgtagaagg atcaggattt gtgttatcat tataatagta aattgctgtg
    12481 ttacttgtag taattgtagc ctgccatcta gttgttgacg gtccataacc aggaacattt
    12541 tcatctatgg caacaactat attgccacca ttccaaacaa caggagtggt aagggtaata
    12601 gtaaaccaac cagcagtagc aggtaaggta atagtacctg tataaacttc tgtgagagat
    12661 gcaaatggca cccaatttgt ggtagaggta aaactcgatt gggtagtctc accaatatat
    12721 acagtccaaa catcggcgtt attcagagtg cctgctccag tccaataaaa acgaatagcg
    12781 tttatttgca tacctgcaac agctccagca gtattaagtt ctgcagaagt gtagatttgt
    12841 tgcgtataat tataaccata aaatgtataa attgggttat atctgccttc ttctgtacca
    12901 gtgccaacag taactgttac ttgagattgt acttgaaaca caaaacagct aaaaaacaga
    12961 atacttagga ataaaatttt cttcatgtta aaaaatatta taaatttcta aaaataaaga
    13021 tgtaaaaatt atatgttcgt tgtctaaaaa caaatttttt tacctattaa tctaaatgca
    13081 aaaataaata aataatttaa ttttcataca aagtttgaca cttttataca ataaaatacg
    13141 ataaagtaat taacaattgg gtgtgaaaat aaattttgat gtaatttgtt tgggattttg
    13201 tgttatggtt ttggcgaaat ttttgaagtt aaacgcaaaa attgtgacaa aacaattata
    13261 aacttatttt gatttttttg atttctgaaa gccttaaatc aggagtttta gtgctataat
    13321 aaaattttat tagtttgtaa ttttgtgata atctattatg ccgtaaatag gattatcaat
    13381 aaatataggt tgttgagttc cgaaatttgc agcagtttta attaaaatgt ctttaaaagc
    13441 atttgcaagt gaaccagtta aacttagttt tttgttttta tagtcattga aaattacaat
    13501 ataattttta aaaaatttgt taaacgaaaa ttctacaagt tctgttaatt gcttgtgttc
    13561 tatgtttttg taaataaaat ggcaaaaatt tgctaaatat ttgtttggat taggattttt
    13621 gtaaacagca tttaatatac ttgctttatt ttcgccagtt tcaaaataaa atttatcgct
    13681 taattctggg tctaatcttt tttctaagta aaattttgca aataactttc caatatatgc
    13741 tccacttcct tcatcgccaa gaatatatcc aagtgaaatt gcattttgct taatagcttg
    13801 tccgttgtaa aagcaagaat ttacgccagt tcctaaaata gcacaaattc cttcatctgt
    13861 ttttaatgat gctcttgcag ctccaagcat atcagaaaaa atactaattt ctgcattaaa
    13921 gaaaaaatct aaaaaagttt gacgaaagtg gtagtaattt tcttcaatag agcaaccaga
    13981 gccataaaaa tataattttt ctatctttaa agagtcgaat ttttgaggaa atgcttctgt
    14041 tagtgtggct aacaattttt gttcacttgt aaaatatggg tttaagccaa tggttttaaa
    14101 ttctgttaaa atattgtatt caaagtccgt tattacccaa tgagtttttg tggaaccgct
    14161 atctgcaaat aaatacataa taattataat taaaaaacga aggctatctc attatgagac
    14221 agcctccatt tgtttttaag ctttttcaaa actacccaac acatttttaa ttttgtgtat
    14281 atataatttt ttcaattctt cgcgagcagg acctaaatat tgacgtgggt caaatttatc
    14341 aggactgtca ttgaagaatt ttctgatagt tgctgtcatt gccaatcttc cgtcagagtc
    14401 aatattgatt ttgcaaacag atgattttgc tgcttctctt aattggtctt caggaatgcc
    14461 tattgcatct tttaaacgcc caccattttc gttgatagtt ttcacatatt cttctggtac
    14521 agatgaagag ccgtgtaaaa caattggaaa tcctggaatt tgtttttcta tttctttcaa
    14581 aatatcgaaa cgtaaaggtg gaggcactaa tttaccattt gcatctcttg tgcattgttc
    14641 tggtgtgaat ttatttgctc catgcgaagt tcctatagat attgctaaag aatcaactcc
    14701 ggttctgcta acaaaatcaa caacttcttc aggttttgta taggtgctgt gttctgccga
    14761 aacgtcatct tctacgccag ccaatacgcc aagttcacct tcaacgctta cgtcaaattt
    14821 gtgagcatat tcaactactt ttcttgttaa agcaacattt tcttcatatt gtaaatgaga
    14881 accatcaatc attacagaag aaaaacccat ttcaatacaa cttttacaaa gttcaaaagt
    14941 atcgccatgg tctaagtgta atgcaatttg tggattttcc caaccgagtt ctttagcgta
    15001 ttcaactgct ccttgagcca tgtatctaag caatgtttga ttcgcgtatt ttcttgcacc
    15061 actcgaaact tgaaggataa ctggggattt tgtttcagca caagctgcaa caatagcttg
    15121 catttgttcc atattgttaa aattaaaagc aggaattgca tatccgcctt ttgtcgcttt
    15181 ttcgaacatt tccttggtgt tcactaagcc taattcttta taacttgtag ccattttttt
    15241 ataattttta aaattgtaat atttaatatt tttggctaag ttaatacttt tacatgtttt
    15301 aagcaaatta aaaatgtaat aattttgtat ttatttgtaa taattaaaat tacttaaatc
    15361 tgtatgaaat acagttgttt agaaaaagta agagccgaaa tttggaattt cggctctatt
    15421 actaacccca atcaaccatg aaaaagagaa aaaatcaaaa ttgaatttga tttctggtgc
    15481 aaagaaaaga aaattaattg tatttgttgt agttttttat taaatattag atttatagtc
    15541 tccttattca gttattctcc attaaaaaac atataaaaag caataaaata aattaacttt
    15601 atttcttcac gtatcaaaat ttttattaac tttgttaaat gttaaaaaac ttagacatat
    15661 ttaatattat ttctaatgat ttagagccgg ctactggaag gttacttatt tcagagccat
    15721 tattgtcaga tgatattttt tcacgttcag taattttaat cgcaggaatg gaaaacaaat
    15781 ctgcgtttgg gtttatttta aataatcctt caaacagaaa tctacacgaa ctaatagacg
    15841 gtgtagaaat agaaaatttg cctgtgttta ttggtggacc agtcgaaaaa gatgttttat
    15901 tttgtattca tactattgat tatctgcccg attcaatcca tataaatgat ggtatttatt
    15961 tgagcggtaa ttttgataca ataaagactt tagtaaattt aggaattata aataattcta
    16021 atgcaaggtt ttttattgga aattctggct gggcacccga acaattacac gatgaaatta
    16081 attttaatta ttggttagtt tccgaaacaa cacgcgagtt tgtttttagc gatttcaaaa
    16141 atcaatggga gcgagcatta gactttgttg ataaaaagta tgaaatctgg aaaaaatttc
    16201 ctaaaaatcc agaatttaat taaagaaact aatcatctat tattataaat aaatcttcgt
    16261 cttcttctaa catttgatat tcttctcttg caaatttttc taaattatct ttatttgaaa
    16321 ataattcttc gtattgtcgt gtgtataagc taatttcgtc ttcgtaatat tttatttctt
    16381 tttttagagc atttagtttt cttaactttt taaatctatc tatcaaatta tatgtatcaa
    16441 aaaatgttac ccataaagca aaaaaaacca aggtaacaac atacttgtgc ttgataataa
    16501 aatttaatgt tttaagtaaa aaattatttt tttcttttat gttttttgta tccataaaat
    16561 aaaactaaat gttgttttca aatttcgtaa ttttgaaagt ataaatatgt ttttttaata
    16621 aaatttatca acaagttgat gtttattcga tttctctaca gcatttctaa aagttcttca
    16681 gcagcgtttt ttacttcttc taaacttttt attttttctt tactgttagc ttttctaact
    16741 gatttaaagt atttgtgtac tacagttttc ataccactaa atcctaaaat acattcttca
    16801 gtcattttgt ttagtgcatc aagtgaggct tggtctttgt aaatattgtt aagccctgta
    16861 gtattgaaaa tagctattct tttgtttttt aattttggaa taatttcgcc atcaataaaa
    16921 tcaaaaccgt atccttgaac aaatactctt tctatatatc cttttaaaat agctggcatt
    16981 cccgaccacc aaattggata tacaaaacaa ataaaatctg cccattgaat aaatttttgc
    17041 tcgtccatta tgtcaacagg aaaaatttct ttttcaatag attctaaatc ctttttgctt
    17101 agaacaggat taaattttat ttcgtaaaga tcgcgaataa caacttcgtg tcctttttga
    17161 tgaaaagtat atgcaatagt atcccttaca gcagaagtaa aactctgctc tttgtaattt
    17221 gcgtatataa gtaatatttt catttttttt taagaatttt attactacaa agataagaaa
    17281 taattgataa ttgaataatt tagttttgaa ttaataaaaa aaaacctgca gaattatatt
    17341 ctacaggttt ttttgttaag tgttaaatat taatttaaaa taatatacta atacacaact
    17401 aatttcactg tattagttgt agcattagct gtagttagta ttttagcata atatacgcct
    17461 gaaccaaatt gttgtaagtt tattgtattt gtattatttt gatttaggat tactctacct
    17521 aaattatcaa aaacctctac agagaatact tgactttcaa tttggaagat accatttgat
    17581 gggtttggat atattgatat cgaacttgaa acactttgta tatttgctga tgaatttgta
    17641 ctaattccaa aacgagctaa cacagcctta tctgttccag atgtgttgtc ataaccagct
    17701 aacagaagtt tgtctgttcc ttgaagtttt atatcaagat agcgaatatc ggcaggtaaa
    17761 gtaaaaactc catcgttggc aaagccttcg ttaagtatta agttaaaatc aaaatttgca
    17821 atttctccat ttttaccacc aataaaaaaa gtgttttcat aaaatgctac aggtaaatct
    17881 aaagcaacaa aattacgaaa ttcagcaatc ccattatata cattcatcat aaggtgtcca
    17941 taaaacttta acataactgt attataaaca ttgcccatta aacttggacc accaaca
//
LOCUS       DAOPNX010000050        17715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_3813_length_17715_cov_6.325991, whole genome shotgun sequence.
ACCESSION   DAOPNX010000050 DAOPNX010000000
VERSION     DAOPNX010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17715
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3813_length_17715_cov_6.325991"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            486..1934
                     /locus_tag="PKN53_06825"
     CDS             486..1934
                     /locus_tag="PKN53_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOD88698.1"
                     /translation="MSPLLIFIIVLSYFGVLLVVSFFTSRNVNNAGFFLGNKKSPWYI
                     VAIGMLGSSISGVTFISVPGWVQTTQMTYLQMVLGFVAGYVVIAHVLLPLYYRLNLTS
                     IYTYLDLRFGRFSYKTGASFFLLSRSIGSAARLYVVANVLQIVIFNQLNIPFWVTVIV
                     TILLIWLYTFKGGIKTIIWTDTLQTIFLFTALIATIIIVARELGLDFSGIVSTINNSE
                     MSKVFEFKDWRSSQHFLKQFLSGAFITIVMTGLDQDVMQKNLSCRNIKEAKRNMLTYG
                     VAFLPANLLFLSLGILLMTFVNSIGIPADLERIAGDNLYPYVATTQLGPVVLILFLIG
                     LMASAYSSADSALAALTTSFTVDIIGIKNKTEQQIKKTRTWVHLAISFLMAIMIIVFN
                     LVNEKSIINAIYAIAGYTYGPLLGLYAFGLFTKYKVRDKIVPIIAILSPILCYLISYL
                     LKKYADYNTGYELLLLNGFITFMGLWAIRKMG"
     gene            complement(1965..3509)
                     /locus_tag="PKN53_06830"
                     /pseudo
     CDS             complement(1965..3509)
                     /locus_tag="PKN53_06830"
                     /inference="COORDINATES: protein motif:HMM:NF021040.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AlwI family type II restriction endonuclease"
     gene            complement(3637..4548)
                     /locus_tag="PKN53_06835"
     CDS             complement(3637..4548)
                     /locus_tag="PKN53_06835"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HOD88699.1"
                     /translation="MKPLIKYRGGKSKEIPYLIKHIPKYNGRYIEPFFGGGALFFHLK
                     PQKAIINDINSKLISFYLGVKNNFDTLKKELTEIEQIYTINRKNFEELKSKTPDKRVE
                     DDNETLYYQIRDMFNELTEKKYSDALLYFFINKTAYSGMIRYNAKGEFNVPYGRYANL
                     NTSLVTKSHSKLLANTEIYNLDYSEIFKMASKNDFMFLDPPYDCVFSDYGNAEHKDGF
                     NEKDHIELAKQYKNLNCKALMVIGRTPLTEKLYKDYIVDEYSKSYAVNIRNRFKAKAS
                     HILITNYGNEALKREPKLFSEESILHG"
     gene            complement(4670..5605)
                     /locus_tag="PKN53_06840"
     CDS             complement(4670..5605)
                     /locus_tag="PKN53_06840"
                     /inference="COORDINATES: protein motif:HMM:NF025454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HOD88700.1"
                     /translation="MELGILKSIEPRQSWENEARDFTPWLANNIAELNKALGLELEVE
                     NTEVCAGPYFADILAKDTSTDNYVVIENQLDKTNHDHLGKVITYASVLDAKTIIWIAT
                     EFTEEHKKALDWLNEHSNDEISFFGVQLELWQIDNSKAAVKFNVISKPNEAVRQAAIS
                     KTYENLSDNEKFLLDFWTNFKERLTKIKKIYSLQIPKPENRFYVNLGKSHIHLTSICN
                     TDNNTVGVYIFISKKIADTIYPFLESIKAEIESAIGQELIWNPNPDNKDKIILLQHTT
                     DFKSEEKIDEALNWLVEYTIKFRETFSRFVKQAPR"
     gene            complement(5803..6273)
                     /locus_tag="PKN53_06845"
     CDS             complement(5803..6273)
                     /locus_tag="PKN53_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88701.1"
                     /translation="MKTQIIIIAICIFSSLNLFAQEHRNKGKACRLDNEKMYAEKVAF
                     ISAELNLSVSEAQNFWPLYNEFNESMSKLFDQEREINHYLKSNLENASEKEISSKLDE
                     LMQIKTDRANLETKYHKKFCKVLPINKVALLYKCDRDFRKHLLRKYKGHKKEEK"
     gene            complement(6284..6718)
                     /locus_tag="PKN53_06850"
     CDS             complement(6284..6718)
                     /locus_tag="PKN53_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88702.1"
                     /translation="MKTLNDIEKKNNFKVPEDYFDNVEKEIIDKINSDQVPKKPTPYQ
                     ILKPYLYMAAAVLILAIGFKGMIKISANKQDKKIITETNEANYTFDDMISELSYYDDM
                     GLYEYINDENDDYWNSELITNDEDLAYLEDYLTQNYLEYELE"
     gene            complement(6753..7301)
                     /locus_tag="PKN53_06855"
     CDS             complement(6753..7301)
                     /locus_tag="PKN53_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOD88703.1"
                     /translation="MTKTKEEDIINLISNPETVKQGFEILVKTYSKQLYFHIRRMLIV
                     HQDADDVLQETWLKAYTQIDKFRFESSIYTWLYRIATNNCLNFLDKKKRQFVFSSYNY
                     EDGLAEKLESDKYFDGDALQLKLKKAVLKLPEKQRLVFNMKYYDEMKYEEISEILRTS
                     VGALKASYHHATTKIEKYINED"
     gene            complement(7308..8261)
                     /locus_tag="PKN53_06860"
     CDS             complement(7308..8261)
                     /locus_tag="PKN53_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOD88704.1"
                     /translation="MSKYTYTEKKDTRSGFGAGLAELGKINNDVVALCADLTGSLKMN
                     EFQKNHPDRFFQAGIAEANMMGVAAGLTIGGKIPFAGTFAGFATGRVYDQIRQAIAYS
                     NKNVKIAASHAGITLGEDGATHQILEDIGLMKMLPNMTVVVPCDYNQTKAATIAIAEH
                     KGPVYLRFGRPAVPNFTDPNQKFEIGKAELLYEGSDVTILATGHLVWKAIEACDILLQ
                     KGIKAEVFNIHTIKPFDKETVLKSIKKTKAMVCAEEHMLNGGLTDTAAQMAALNFPIP
                     IEAVAINDTFGESGTPDQLMEKYGLTTNNIVQAVEKVLKRK"
     gene            complement(8273..9664)
                     /locus_tag="PKN53_06865"
     CDS             complement(8273..9664)
                     /locus_tag="PKN53_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOD88705.1"
                     /translation="MKKIVISILLVLGFFINLNAQIKNYPEPEKPLTRILIIFDASTS
                     MMQQWEGGLKIVKAKELLNELLDSLASVQKTQDLEVAFRVYGHQSPDHLMDCFDSKLE
                     VSFGANTIGMIKDRLKTIQATGTTPIAYSLGKAEYDFPECSDCRNIIILITDGLEMCD
                     GVPCEVSVALQKKGVVLKPYIIGIDIDIRVSDLLSCIGNYFDASSEKEFKRAINTIVG
                     QVTNLTSVQVNLLDTHGRPTETNVAMSFHDNFSGDIRYTYMHTLNKSGQPDTIYLDIL
                     SIYDLKVHTIPPVYLDSIKLEEGIHNIISVPAPQGYLFVDIDGVAPENSQIKCIVRQT
                     GLPQTMYILDADKKQKLITGLYDLEILTTPRTYVDAVLIEQSKTKKITIPEPGDLIIN
                     FKNPAFASLLYEKGDVLTNLYDFTPDKTHYKFRLQPGYYRIIFREQRHSKSTNTGEFK
                     FRIKSGEFRVENL"
     gene            complement(9675..10517)
                     /locus_tag="PKN53_06870"
     CDS             complement(9675..10517)
                     /locus_tag="PKN53_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOD88706.1"
                     /translation="MENIQKLEKIASQVRRDLLRMIHAKKSGHPGGSLGVTDLLVCLY
                     FEIMDHNKDFKMDGKNEDIFFLSIGHTSAAWYSVLARSGYFPVKELASFRAIDSRLQG
                     HPATAEGLPGVRIASGSLGQGLSNACGAALAKKLNNDKKFVYCLLGDGEIQEGQVWEA
                     ALFAQARKIDNLIAFIDYNGQQIDGTIEDVMNLKNLRQKWEAFNWIVLECNGNNMQEL
                     ISTIKKAQELSGKEQPIMILSKTEMGHGVDFMTGSHKWHGVPPSDEQLENALSQLEET
                     LGDY"
     gene            10624..11868
                     /locus_tag="PKN53_06875"
     CDS             10624..11868
                     /locus_tag="PKN53_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD88707.1"
                     /translation="MNIELYLAKRIVSRKHAMNSFSRPIIAVAIGGIAIGLAVMILSI
                     AIVTGFKNEITNKVVGFGSHLQIVNFDNNTSYETYPISKSQTWLSELKQIEGVESVNM
                     FITKAGIAKTDEDLQGVIFKGIDNDFDWKFFKQYLTEGDTLLISDSSRNNGVVVSASI
                     ANALKLNVGDSFSAYFIQEPPRMRKFTIIGIYETKLEDLDKIFVLCDIKHLRKLNDWK
                     DDEITGFEVKIQDFSKIMDMEIKVSRLVGNYLSADGGMIKVKSIVEDYPGIFDWLSLL
                     DINVWVILILMLSVAGFNMISGLLVIILERVNMIGILKSIGASNVKVAKVFIYVAGFL
                     ISRGMLIGNIIGIGLCLLQKYFGIITLDADSYYVSVVPINLDLIHLLLLNIGSLVITL
                     IMMILPSMLVSKISPAETIRFN"
     gene            11965..13212
                     /locus_tag="PKN53_06880"
     CDS             11965..13212
                     /locus_tag="PKN53_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOD88708.1"
                     /translation="MAVVKPFKGLRPPKHLVENVSCLPYDVMNSAEAAQMAKGKKESL
                     LHVTRAEIDLPAGTDIHSSVVYEKAVENFEKMQKEGWLVQDKEAKFYIYAQTMEGRTQ
                     YGIVGCAFYEDYYNGVILKHELTRPDKEEDRMTLTKYTNANIEPVFFSYRAVDELDAI
                     IDNIVKNQEPEYDFVAEDGFGHHFWVIRDAKINQRIEELFATKVPVFYVADGHHRTAA
                     ASRIGKERAENNPNHTGKEEYNYFMAVIFADKQLKIMDYNRVVQDLNGLSQEEFLTKV
                     ADKFYMQKMGTEIYKPAALHEFSMYIDGNWYKLNAKPGTYNDDDPIEILDVNVLSKYV
                     LDEILGIKDLRRSNRIDFVGGIRGLGELKKRVDSGEMKVAFALYPVSMDQLLNISDTG
                     NIMPPKTTWFEPKLRSGLVIHKL"
     gene            13278..14381
                     /locus_tag="PKN53_06885"
     CDS             13278..14381
                     /locus_tag="PKN53_06885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD88709.1"
                     /translation="MILSWISIALISIYLIVLLIFTFAFIKRKTFAEKCNDFSKKLSV
                     IIAFRNEKANLKQLIDSLLNQNNAPNFEIILVNDHSEDNFLEVLNSFSDERIKLYNIT
                     EEFTGKKAALRFGISKSQGEILFFTDADCVLPKNWISTMLAYQQKNNVQMLCGPVKFK
                     DSNKFLEKIFALEFMSMTGSGAAGFFIDKAFMCNGANYCICKSILNEVGADFNNEFSS
                     GDDVFLLHYVSSKYKVDFLKSYDACVETSAPSSLQEFFNQRIRWASKISGYRNAFSIF
                     VALATYFSAFSLLFLVILTLFFTKFYALLIAVLVAKFVVEILFLIPISKFYKQSNCLI
                     YLPLVQLFHPLYVVFIPLIPFFYKPKWKNRKII"
     gene            complement(14383..16566)
                     /gene="recQ"
                     /locus_tag="PKN53_06890"
     CDS             complement(14383..16566)
                     /gene="recQ"
                     /locus_tag="PKN53_06890"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOD88710.1"
                     /translation="MTDKEKQIFEVLKKYFGFDNFKGQQKEVILSLLDGHDSFVLMPT
                     GGGKSLCYQLPALILEGTAIVISPLIALMKNQVDAMRGFSLDSGVAHFLNSSLNKTET
                     NIVKEDVLSGKTKLLYVAPESLTKEENVEFMKKFKISFFAIDEAHCISEWGHDFRPEY
                     RRIRPIVDAIGRTPLMALTATATPKVQHDIQKNLDMLDAKVFKASFRRHNLYYEVRPK
                     INASKEIIKYIKNNPGKSGIIYCLSRKKVEELAELLVVNDIKALAYHAGMDAAVRSQN
                     QDMFLNEEANVIVATIAFGMGIDKPDVRFVIHYNIPKSLEGYYQETGRAGRDGGEGKC
                     ITFYSYDDIQKLEKFMQGKPVSEQEIGKQLLFDTVSYAESSVCRVRQLLNYFGEEMKE
                     NCGNCDNCLNPKPKFEGKEDILTVLETIMLVKEKFKAEHIVNILTGKKTAEIATFKHQ
                     DLPIFGTGGQDQARHWKAVIRQMLIAQFIEKEIVNYGLLKITDKGRDYYERPYSVLLT
                     KDHNYEDAEDDNEGGMFVGGSATDEELFAMLKDLRKSIAKKHKLPPYVIFSDPSLQDM
                     VIQYPITMDELTNITGVGQGKAQKYGEEFIKLISKYVEENEIERPQDLVIKSVVNKST
                     NKVFIIKNIDRKMPLEEVCKALGIEMDELIEEIEAIVNSGTKIDIDYYLRSILDEEII
                     DDIFAYFREEAESDSIEDAIADLEDKYSEEEIRLVRIKFLSEVAN"
     gene            16756..>17715
                     /locus_tag="PKN53_06895"
     CDS             16756..>17715
                     /locus_tag="PKN53_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOD88711.1"
                     /translation="MQKNNIEKIIEIAKKTIVLEAESISNLVNYIDKSFAEVVNKIFE
                     STGRVIITGIGKSAIIGNKIVASLNSTGTPAIFMHAADAIHGDLGIVQKDDVVICLSK
                     SGNTPEIKVLIPLIKNNGNLLVAIVGDENSFLAKSADYVLLTTVDSEACPNNLAPTSS
                     TTAQLVMGDALMLALLQMRGFTAKDFARFHPGGSLGKKLYLRVDDIYIQNAKPKVKPN
                     DNITKVISEITGKRLGATAVVDENDMMLGIITDGDLRRMLEKYPSEFINLKAKDIMSD
                     KPKMVEKDCLAINALSVIKANNISQVVVVEDNKYLGIVHMHDLM"
BASE COUNT         5550 a         2660 c         2798 g         6707 t
ORIGIN      
        1 cagttggtta gagcacctga ctgttaatca gggggtccta ggttcaagtc ctagaagggg
       61 agctgttttt agaaaaggct tacacgaaat tgtaagcctt ttttttgtcg ttctcataac
      121 tcgctcggat ttgcaatcga gtggtggtat ttacactcgg attacaaatc cgagcgagcg
      181 tcatgggtgt tgagagcgag cgtcatgggt gttgagagcg agcgtcatgg gtgttggagc
      241 aagcaggtgt tgtaattgca agcagagtga gcttgcgaac tctgcgaagc aatagacgcg
      301 tcagcctact tttaacactt caactacgtt cagtgcatcg cttttaacac ttcaactacg
      361 ttcagtgcat cgcttttaac ttttaactga ttttgtatta acattgcata agtgaaaact
      421 ttttaatttt ttacatagat actagttttt atttgttgaa atttgtaaaa atttaaaatt
      481 actatatgag tccgctattg attttcataa ttgtactaag ttattttggg gtattgctgg
      541 tagtgtcgtt ttttacatct cgaaatgtaa ataatgctgg ctttttctta ggtaataaaa
      601 aatcgccatg gtatattgta gcaattggaa tgttgggttc ctcgatttcg ggagttactt
      661 ttatttccgt tccaggttgg gtgcagacca cgcaaatgac ttatttacag atggttctgg
      721 gttttgtggc tggttatgtc gtaattgctc atgtactttt accattatat tataggttaa
      781 atttaacttc gatttacact tatttagatt tacgtttcgg aagattttca tataaaacag
      841 gtgcttcttt tttcttactt tcaaggtcaa ttggctcggc tgcacgtctt tatgtggttg
      901 caaatgtctt gcaaatagta atttttaatc aattaaatat tccattttgg gtaacggtta
      961 tagtaacaat tcttttaatt tggctatata cttttaaagg cggaataaaa acaataattt
     1021 ggacagatac tctgcaaaca atttttcttt tcactgctct tattgcaact ataataattg
     1081 tagctcgtga attaggtctt gatttttctg gaattgtttc tactataaat aatagcgaaa
     1141 tgtcaaaagt ctttgaattt aaagattggc gttcgtctca gcattttcta aaacaatttt
     1201 taagtggtgc gtttatcact atagtaatga ctggcttaga ccaagatgtg atgcagaaaa
     1261 atttatcttg ccgaaatatt aaagaagcaa aacgaaatat gctgacttat ggtgtagcat
     1321 ttttacctgc aaatctattg tttttatctc ttggaatttt attgatgact tttgtaaatt
     1381 ctattggaat accagcagat ttagaacgta ttgcaggcga taatttatat ccttatgttg
     1441 caactactca attaggacct gttgttttaa ttttattctt aataggttta atggcttcgg
     1501 catattcaag tgccgactct gctttagctg ctcttacaac ttcgtttaca gttgatatta
     1561 ttggaataaa aaataaaacc gagcaacaaa ttaaaaaaac aagaacttgg gttcaccttg
     1621 ctatttcttt cttaatggca ataatgataa tagtgtttaa tctcgttaac gaaaaaagca
     1681 ttataaatgc aatttatgct attgctggct atacttatgg accactctta ggactttatg
     1741 cttttggttt atttacaaaa tataaagtaa gagataaaat cgttccaata atcgcaattt
     1801 taagtcctat actttgctat ttaatttctt atcttttgaa aaaatatgca gattataata
     1861 caggctacga actcttactg ttgaacggct ttattacttt tatgggactg tgggcaatca
     1921 gaaaaatggg gtagagggaa aggtggaaga aagtaaatag ttttttattc tgctaaccaa
     1981 tttagagctt tttgggtaat tccattatac caatctattt cgttagttgt tgagtttgct
     2041 aattcgtttg aacgctcaaa aaatcgttta acatgtcttg gttcaggaac gtagtttgcg
     2101 ttatgagagt cttgaatcat tttgataaaa acactcaaag gcaatggaac tattgtagaa
     2161 gttccaccat aatatttaat gttcattttg tgcaatgcgt aaaaatgagc tttgcaggca
     2221 tcatttattt ttggtgcaat gaataaacaa tatgctggtt ttttagtttc ttttttgaac
     2281 tttgcaagat gtcttgttac tggttctcct tctgtttcgt attgtctttg tccagtctgc
     2341 atagtaactt ctactgtcaa accgaagtcg ccataatcgc aaacaatatc agccatatta
     2401 ccttgtgctg ttgacatggg attgccaaag tcgtcaaatt ttaaattggc tttaatctta
     2461 ccaccatcta acattgtcat tgctcgccaa gtgttccact ccaacataag tggtgtgtca
     2521 taaagtgaat tgtcaagtat ttgctcaaat attgtagaaa tatcttcaaa aagtcggtag
     2581 tctttaattg caacaatttg ttcagttata attttgtttt ttctattttc caactcgtca
     2641 gcaaatatat ttttaagctc ttgtaatgtt aaagagtcag aaatttcaag ttgcggaaac
     2701 tctgctttaa tttttttctc taataattcc ctgttgtctg ataaaagtgc tggaataagc
     2761 ggattgccaa gataagtaat atattgactt tcattattga taaagcaagg ctctctatct
     2821 gtatgttgta agaaataatc aacttcttgg attttttctc gaattattga gattgatttc
     2881 cctagatatg aaatatttac caaacctgtt gctctcaaat atcggaaaca cgcatcggta
     2941 taatctcgca tattacttgc ttttgtgcta aggaatttcg caatcgaagc atcattggtt
     3001 tctctagttc gtgtatctcc agaacttatt ttgtctttat aaatttctct taattctttg
     3061 tcaaaaacat ttgctcggaa acgtttatag tttccttagt tttgtgtttt tgcaattcta
     3121 aattggttta ttttttcaat tattgtatca aaatcacggt aatcaactaa ttgtaaaccg
     3181 aatatcatta actcatcaaa ttttaaagag ccaaaatgtc taataagcct gaagaattct
     3241 aaatagggtt ttatccaaaa atttgctgaa ttttcagttg gtttatgaaa tggtgaagga
     3301 acttgaaatt ttaataattg tcttaaaaat atttcatctt ttctttttga gtttacaagt
     3361 tcttcgcctg ctggagtaag cttaatagtt ggagcaagaa ttacaaagcc aagtgctttt
     3421 ggagctctat ttatcctatc tcttgcacta aatgcagggt catttgcacc tttgccattg
     3481 aaaaaatttt cttctctcaa tagctccata aatgctcttt gagtttttgt attccatttt
     3541 tgacctgcga aatgagtgtg caaaagttca atttcgggaa tcatttttgc aggtgttcgt
     3601 ggagatgtgg ttacaaaaat tactttacta ttaattttag ccatgtaaaa tagattcttc
     3661 tgaaaataat ttaggttctc gttttaatgc ttcattacca tagttagtaa taagaatatg
     3721 acttgctttt gccttaaatc tattcctaat atttactgcg taagacttac tatattcatc
     3781 aacaatatag tctttatata atttttccgt tagtggcgtt cttccaataa ccataagtgc
     3841 cttacaattt aaatttttat attgtttggc tagttcaata tggtcttttt cattgaatcc
     3901 atctttatgt tcagcattgc cataatctga aaaaacacaa tcataaggtg ggtctaaaaa
     3961 cataaagtcg tttttagatg ccatcttgaa aatttcagaa tagtcaagat tataaatttc
     4021 tgtattagca agtaatttac tgtgagattt tgtaactaat gaagtgttta gatttgcata
     4081 tcgaccgtaa gggacattaa attctccttt tgcattatag cgtatcattc ctgaatatgc
     4141 agttttattg ataaaaaaat aaagcagagc atcagagtat tttttttcag ttaactcatt
     4201 aaacatatct ctaatttgat aatagagtgt ttcgttatca tcttcaactc gcttgtcagg
     4261 tgttttgctt ttaagctctt caaagttttt acggttgata gtgtaaattt gctcaatttc
     4321 tgttaactct tttttaagtg tgtcaaaatt attttttaca cccaagtaaa acgaaatgag
     4381 ttttgaatta atgtcattta ttattgcttt ttggggtttt aaatgaaaaa acaaagctcc
     4441 accaccaaaa aatggttcaa tatatcgtcc attgtatttt ggaatatgtt taatcaaata
     4501 aggtatttct ttagattttc ctcctctgta ttttataagt ggcttcatac tttgtctaaa
     4561 ttatgtttgt atagaacaat attttgtaca cattcaatct tttaaggtaa ttttcacaaa
     4621 acaaatatac aacattccta aatattaagc aaataaaatt tgagcattat tatctcggtg
     4681 cttgttttac aaatcttgaa aatgtttctc taaattttat ggtatattca acaagccaat
     4741 tcaatgcttc atctattttt tcttcgcttt taaaatctgt tgtatgttgc agtagaatta
     4801 ttttatcttt attgtcagga tttggattcc atattagttc ttgcccaatt gcggactcaa
     4861 tttctgcttt tatactttct aaaaatgggt aaatagtatc tgcaattttt ttgcttatga
     4921 aaatatacac accaactgta ttgttatcag tattacaaat gcttgtaagg tgaatatgcg
     4981 attttcctaa atttacgtaa aacctatttt caggtttcgg aatttgaaga gaataaatct
     5041 ttttaatttt tgttaatctt tctttgaaat tagtccaaaa atcaagtaga aatttttcat
     5101 tatcagacaa gttttcataa gtcttagaaa ttgctgcttg tctaacagcc tcatttggtt
     5161 tgctaatcac attaaatttt acagcagctt tactattgtc aatttgccac aattctaact
     5221 gcactccgaa aaatgaaatt tcatcatttg aatgctcatt aagccaatcc aatgcttttt
     5281 tatgctcttc tgtaaattca gtggctatcc aaataattgt ttttgcatct aatacagaag
     5341 catatgtaat tactttgcct aagtggtcgt ggtttgtttt gtctaattga ttttcaatga
     5401 cgacataatt atctgtacta gtatcctttg ccaatatgtc tgcaaagtaa ggaccagcac
     5461 aaacttctgt gttttcgact tcaagctcta atccaagagc tttgtttaat tctgcaatat
     5521 tgtttgcaag ccaaggggta aagtctcttg cttcattttc ccatgattgt cttggctcaa
     5581 tactttttaa aattcctaat tccatagcat ttaaattttg attttttaca aacttaatgc
     5641 tgaactacgt caaaaagtgt cgtgctgtaa ttttttttta attaaaaata attttaaaaa
     5701 aaacaataga aaataataag caaataatat ttgatatatg ccaataaaaa atagaaattt
     5761 taagcttgtt ttagtgtaga aatcagaaga ataaaatcaa atttacttct cctctttttt
     5821 atgtccttta tatttgcgta ataagtgttt gcggaaatct ctatcgcatt tgtaaagtag
     5881 ggcaacttta tttataggta aaactttgca aaacttttta tgatatttag tttctaaatt
     5941 ggctctgtcg gttttgattt gcattaattc gtcaagtttg cttgaaattt ctttttcact
     6001 tgcattttct aaatttgact ttaaatagtg atttatttct ctttcttggt caaatagttt
     6061 gctcatgctt tcattaaact cattataaag gggccaaaag ttttgtgctt cgcttacgct
     6121 caaattaagt tctgcactta taaatgcaac tttttcagca tacatttttt cattatctaa
     6181 tctgcaagct tttcctttat ttctgtgttc ttgtgcaaaa agatttaaac tgctaaaaat
     6241 acatatagca ataattatta tttgtgtttt catgttgttt attttattca agttcatatt
     6301 ctaaataatt ttgtgttaaa taatcttcta aatatgcgag gtcttcatca ttagtaatta
     6361 attcagaatt ccaataatca tcattttcat cgttgatata ttcgtaaagt cccatgtcgt
     6421 cataataact tagctcagaa atcatatcat caaaagtata attagcttcg ttagtttctg
     6481 taattatttt tttgtcttgc ttgttagcac tgatttttat catgccttta aatccaatag
     6541 ctaaaataag aaccgcagca gccatgtata aatatggctt taaaatttga tatggagtag
     6601 gctttttagg tacttggtct gagtttattt tgtcgataat ttctttttct acattatcaa
     6661 aataatcctc tggtacttta aaattattct ttttttctat gtcattaagt gtcttcataa
     6721 tcttaaatta gacagtttaa aactattaag gtttaatctt cgttaatata tttttcaatt
     6781 tttgttgttg catgatgata cgaagctttt aaagctccaa cgcttgtcct tagaatttct
     6841 gaaatttctt catatttcat ttcgtcgtaa tacttcatat taaaaaccaa tctttgtttt
     6901 tcgggaagtt ttaaaaccgc ttttttaagt tttagttgta aagcatcgcc atcaaaatat
     6961 ttatcgcttt ctaatttttc tgccaagcca tcttcgtaat tgtatgatga aaaaacaaac
     7021 tgccttttct ttttgtctaa aaagtttaag caattatttg tagcaattct gtataaccaa
     7081 gtgtaaatcg acgattcaaa tctaaattta tcaatttgag tatatgcttt taaccaagtt
     7141 tcttgtaaga catcatcagc atcttggtgt acaattagca tacgtcttat atgaaaatat
     7201 aattgttttg aataagtttt tactaaaatt tcaaagcctt gctttacagt ttctggatta
     7261 gaaattaaat taattatatc ttcttctttg gttttcgtca tcaaaaatta tttccttttc
     7321 aaaacttttt caacagcttg aacaatatta ttagttgtta aaccgtattt ttccatcaat
     7381 tggtctggcg ttccgctttc tccaaaagtg tcgtttatag caactgcttc tattggaata
     7441 gggaagttaa gtgctgccat ttgagctgct gtatctgtta aaccgccatt tagcatatgt
     7501 tcttctgcac aaaccatagc tttagttttc ttaattgatt ttaagacagt ttctttatca
     7561 aaaggtttaa ttgtatgaat attaaaaact tcggctttaa ttcctttttg taataaaata
     7621 tcgcaggctt caattgcttt ccacactaaa tgtcctgttg ctaagatagt tacatcgctt
     7681 ccttcgtata ataattctgc ttttccaatt tcaaattttt ggtttgggtc agtaaagtta
     7741 ggcacagcag gacgaccaaa tctaagatat acaggtcctt tatgttcggc tattgctatt
     7801 gtagcagctt ttgtttgatt ataatcgcaa ggaacaacaa cagtcatatt cggtagcatt
     7861 ttcatcagtc ctatatcttc taaaatttga tgagtagctc catcttcgcc taaagttatt
     7921 cctgcatgtg aagcagcaat ttttacgttt ttatttgaat atgcaatagc ttgacgaatt
     7981 tggtcgtaaa ctctacctgt tgcaaatcct gcaaaagttc ctgcaaaagg aatttttccg
     8041 cctatagtta agcctgcagc aactcccatc atgtttgctt ctgcaattcc tgcttgaaaa
     8101 aatctgtcgg gatgattttt ttgaaactca ttcattttta atgaaccagt taggtcggca
     8161 cacaaagcaa caacatcatt gttaattttt cctaattctg caagtccagc tccaaatcct
     8221 gaacgagtgt cttttttttc tgtataagta tatttgctca tcttgttatg ttttataaat
     8281 tttctactct aaattctcct gatttaattc taaatttgaa ctcacctgtg ttggtactct
     8341 tagaatgtct ttgttctcta aaaataattc tgtaatatcc tggttgcaat ctaaatttat
     8401 aatgtgtttt atctggtgta aaatcgtata aattggttaa tacatctcct ttttcgtaaa
     8461 gtaaacttgc aaaagcagga tttttaaaat ttattattaa atctcccggt tctgggatag
     8521 tgattttttt tgtttttgat tgttctataa gcactgcatc aacataagtt ctgggcgttg
     8581 ttaaaatttc taaatcataa agtccagtaa taagtttttg ctttttgtcc gcatctaaaa
     8641 tatacatggt ttgtggtagc ccagtttgac gaacaataca ttttatttga gaattttcgg
     8701 gcgcaactcc gtctatatca acaaataaat atccttgtgg tgcaggcaca cttataatat
     8761 tatgaatgcc ttcttctaat tttatgctgt ctaaataaac aggcggtata gtgtgaactt
     8821 ttaaatcata aatagaaagt atatctaaat atatggtgtc tggttgacct gatttgttta
     8881 gagtgtgcat gtaggtatag cgtatatcgc cagaaaagtt gtcgtggaaa gacattgcta
     8941 cgtttgtttc ggtaggtctt ccgtgagtat ctaataaatt tacttgaacg cttgtaaggt
     9001 ttgtaacttg tccaacaata gtattgatgg ctcttttaaa ttccttttcg ctacttgcat
     9061 caaaataatt tcctatacaa gataataagt cagaaactct aatgtcgata tcaatgccaa
     9121 ttatatatgg tttaagaact acaccttttt tctgcaaagc aacacttact tcgcaaggaa
     9181 ctccgtcaca catttctaat ccgtctgtga taagtataat aatatttcta cagtcagaac
     9241 attctggaaa gtcgtattct gcttttccaa gagagtaggc gataggtgta gttcctgttg
     9301 cttgaatagt tttaagcctg tctttaatca ttcctattgt atttgctcca aatgaaactt
     9361 caagttttga gtcgaaacaa tccatcaaat ggtcgggact ttggtgtccg taaactctaa
     9421 aagcgacttc taaatcttga gttttttgaa cgcttgctaa tgaatctaat aattcgttta
     9481 atagttcttt agcttttaca atttttaaac cgccttccca ttgttgcatc atgctggtag
     9541 aagcgtcgaa aattattaaa attcttgtta aaggtttttc tggctcagga taatttttta
     9601 tttgagcatt aaggtttata aaaaagccta aaacaagtaa tatggatata actatttttt
     9661 tcaaaattta tattttaata atcacctaaa gtttcttcaa gttgcgaaag tgcattttct
     9721 aattgttcgt cgcttggtgg aactccatgc catttgtgcg agcctgtcat aaagtcaact
     9781 ccatgtccca tttcagtttt cgacaaaatc ataattggct gttcttttcc tgatagttct
     9841 tgtgcttttt ttattgttga aataagctct tgcatattat ttccgttgca ttctaaaact
     9901 atccaattaa atgcttccca tttttggcga agatttttta aattcatcac atcttcaatt
     9961 gttccatcaa tttgttgtcc gttgtagtcg ataaaagcaa ttaggttgtc aatttttcgt
    10021 gcttgtgcaa acaaagctgc ttcccatact tgtccttctt gaatttcgcc atcgcctaac
    10081 aagcaataaa caaatttttt atcattattt aatttttttg caagagctgc tccacaagca
    10141 tttgacaaac cttgacctaa agagccagat gcaattctca ctccgggcaa tccttcagca
    10201 gtagcaggat gtccttgaag tcgagaatct atagctctaa aacttgctaa ttcttttact
    10261 ggaaaatatc ctgaacgtgc taaaacacta taccatgcag cagaagtgtg accaatggat
    10321 aagaaaaata tatcttcatt ttttccgtcc attttaaaat ctttattatg gtccattatt
    10381 tcaaaataaa gacatactaa aaggtctgta acgccaagcg aaccaccagg gtgtcctgat
    10441 ttttttgcat gaatcattct aagtaagtct ctgcgaactt gacttgcaat tttttctaat
    10501 ttttgaatat tttccatttc tatttttatt tttgcacaaa ggtaggataa taaattgaaa
    10561 aatttaaata aataatgatt gaatttggat taaagtttat acttttacat aaaatatttt
    10621 gatttgaaca tagaacttta tttagccaag agaattgtta gcagaaaaca tgctatgaat
    10681 agcttcagtc gtcctattat tgccgtagct ataggcggta ttgctattgg cttggcggta
    10741 atgattttat ctattgctat tgttacggga tttaaaaatg aaattacaaa taaagttgtt
    10801 ggttttggtt cgcatttgca aatcgtaaat tttgataata atacttctta cgaaacttat
    10861 cctataagca aatcacaaac ttggctttct gaacttaaac aaattgaagg tgtagaatcc
    10921 gtaaatatgt ttataactaa agccggaatt gctaagacag acgaagattt gcaaggcgtg
    10981 atttttaaag gaatagataa tgattttgat tggaaatttt ttaagcaata tttaaccgaa
    11041 ggcgatacat tattaataag tgattcgagt agaaataatg gagttgttgt ttctgcgagt
    11101 attgcaaatg cactaaaatt aaatgttggt gatagtttct cggcttattt cattcaagag
    11161 ccaccacgaa tgcgaaagtt tactataatt ggaatttatg aaactaaatt agaggattta
    11221 gataaaattt ttgtcttatg tgatattaag cacttacgca aacttaatga ttggaaagat
    11281 gatgaaataa ctggatttga agttaaaatt caagattttt ctaaaataat ggacatggaa
    11341 ataaaagtca gtaggttagt gggtaattat ttaagtgctg atggcggtat gattaaagtt
    11401 aagagcattg tagaagatta tccaggaatt tttgattggc tttctttatt agatataaat
    11461 gtttgggtaa ttttaattct aatgctgtca gtagcaggct ttaatatgat ttcgggtttg
    11521 cttgtgataa ttttagaaag agtaaatatg ataggtatat taaaaagtat tggtgcttca
    11581 aatgttaaag ttgcaaaagt ttttatctat gtggcgggct ttttaatttc gcgaggaatg
    11641 cttatcggaa atattattgg aataggtttg tgtttattgc aaaaatattt tggaataatt
    11701 actttagatg cagattctta ttatgtatct gtagttccaa tcaatttaga cttaattcat
    11761 cttttgttat taaatattgg ttctttagtt attacactga taatgatgat tttaccttca
    11821 atgttagtgt caaaaattag tcccgcagaa acaataagat ttaattaatt gatttttttc
    11881 gcatattatt taacacattt tttattttgt ataaaaaaac tatatttgcg aaaaaatttt
    11941 tagtaacttt taaaattata taaaatggca gtagtaaaac cttttaaagg attgcgtcca
    12001 ccaaaacatt tggttgagaa tgtttcgtgt ttaccttatg atgtgatgaa tagtgcagaa
    12061 gcagcacaaa tggcaaaagg caaaaaagaa tctcttttgc atgtaactcg tgccgaaatt
    12121 gacttaccag caggaacaga tattcatagt agcgtagttt atgaaaaagc tgttgagaat
    12181 tttgaaaaaa tgcaaaaaga aggctggctt gttcaagata aagaagctaa attttatatt
    12241 tatgctcaaa ctatggaagg acgtactcaa tacggtattg ttggttgtgc tttttatgaa
    12301 gattattata atggcgtaat tttaaaacat gagttaactc gtccagataa agaagaagac
    12361 agaatgactt taacaaaata tacaaacgct aatattgagc ctgttttctt ttcttacaga
    12421 gcagttgatg aacttgatgc aattatcgat aatattgtaa aaaatcaaga gccagaatac
    12481 gattttgttg ctgaagatgg atttggacat catttctggg taattagaga tgcaaaaata
    12541 aatcaaagaa tagaagaatt atttgcaact aaagttcctg tgttttatgt ggctgacgga
    12601 catcacagaa ctgctgcagc aagcagaata ggaaaagaaa gagccgaaaa taatcctaac
    12661 catacaggaa aagaagaata taattatttt atggctgtta tttttgcaga taagcaactt
    12721 aaaattatgg actacaatcg tgtagtacaa gatttgaacg gtttatcgca agaagaattc
    12781 ttaacaaaag ttgcagataa attttatatg caaaaaatgg gtacagaaat ttacaaacca
    12841 gctgctttgc atgagttctc aatgtatata gatggtaatt ggtataaact taacgcaaaa
    12901 cctggtactt ataacgatga tgacccaatc gaaattcttg acgttaatgt gctctcaaaa
    12961 tatgtacttg atgaaatttt aggaattaaa gatttgagac gtagcaatag aattgacttt
    13021 gttggtggaa ttagaggttt aggcgaattg aaaaaacgcg ttgattctgg cgaaatgaaa
    13081 gtagcttttg ctttatatcc agtctctatg gaccaattat taaatatttc tgatacagga
    13141 aatattatgc ctcctaagac tacttggttt gagcctaagt tgcgttctgg attagttata
    13201 cataaattat aaaacaacaa aatattaaaa aaagagtttg ttgcgagaca aactcttttt
    13261 ttatttataa aagtttgatg attttatctt ggataagcat agcactaatt tcaatctatt
    13321 tgattgttct tttaatattt acttttgcat ttattaaaag aaaaactttt gctgaaaagt
    13381 gtaatgattt ttcaaaaaaa ctaagtgtta ttattgcatt tagaaacgaa aaggctaatc
    13441 ttaaacaact aattgatagt ttattaaatc aaaataatgc tccaaatttt gaaattattt
    13501 tagtaaacga ccatagcgaa gataattttt tagaagtttt gaattctttt tctgatgaaa
    13561 gaataaaact ttataatata accgaagagt ttacaggcaa aaaagcagca ttaaggtttg
    13621 gaatttcaaa atctcaaggc gaaattttat tttttacaga tgccgattgt gttttgccta
    13681 aaaattggat ttcaacaatg ttggcatatc aacaaaaaaa taatgtgcaa atgctttgtg
    13741 gacctgtgaa atttaaagat tctaataaat ttctagaaaa aatatttgct ttagaattta
    13801 tgagcatgac tgggagtgga gcagcaggat tttttataga caaagcattt atgtgcaatg
    13861 gtgcaaatta ttgtatttgc aaaagcattt taaatgaagt aggagcagac tttaataatg
    13921 aattttcttc aggtgatgat gtgtttttat tgcattatgt atcgtcaaaa tataaggtag
    13981 attttttgaa atcttatgat gcttgtgttg aaacatctgc tccgtcttct ttacaagagt
    14041 tttttaatca gcgaattaga tgggcttcta agatttcggg ttatagaaac gcattttcaa
    14101 tttttgtggc tcttgcaaca tacttttcgg cattttcttt acttttttta gttattttaa
    14161 ctttgttttt tacaaaattt tatgctttgt tgattgcagt tcttgttgca aaatttgttg
    14221 ttgaaatatt atttttaata cctataagta aattttataa acaaagtaat tgcttgattt
    14281 acttgccatt agtgcaattg tttcaccctt tatatgtggt atttattcct ttaattccat
    14341 ttttttacaa accaaaatgg aaaaatcgta agattatata aattagttag ctacttctga
    14401 caagaattta attctaacta atctaatttc ttcttcgcta tatttgtctt ctaaatcagc
    14461 aattgcatct tcaatagaat ctgattctgc ttcttctcta aaatatgcaa aaatatcatc
    14521 tataatttct tcatctaata tagaccttaa ataataatca atatctattt ttgttccaga
    14581 atttactata gcttcaattt cttcaattag ttcgtccatt tctattccta atgctttgca
    14641 gacttcttca agaggcattt ttctatcaat gtttttaata ataaaaactt tgttagttga
    14701 cttattaaca acagatttta taacaagatc ttgaggtctt tcaatctcat tttcttcaac
    14761 atatttacta ataagcttaa taaattcttc gccatatttt tgagcttttc cttgtcctac
    14821 accagtaatg tttgttagtt cgtccatggt aataggatat tggataacca tatcttgcaa
    14881 tgaagggtcg ctaaaaatta cataaggtgg taatttatgt ttttttgcta ttgattttct
    14941 taaatctttt agcattgcaa acaattcttc atctgttgcc gaaccgccta caaacattcc
    15001 tccttcgtta tcatcttcgg catcttcgta attatggtct tttgttaata aaactgaata
    15061 tggtctttcg taataatctc ttcctttgtc agtaattttt aaaagtccgt aattaactat
    15121 ttctttttca ataaattgag caatcaacat ttgacggata actgctttcc aatgtctggc
    15181 ttggtcttgt ccacctgttc caaaaattgg taaatcttga tgtttaaaag ttgcaatttc
    15241 tgcagttttc ttgcctgtaa gaatatttac aatatgttcg gctttaaatt tttctttaac
    15301 aagcattatc gtttccaaaa cagttaaaat atcttcttta ccttcgaatt taggttttgg
    15361 atttaagcag ttatcacagt ttccgcaatt ttctttcatt tcttcgccaa aatagtttaa
    15421 gagttgtcta actctacaaa cacttgactc tgcatatgaa acagtatcga aaagtaattg
    15481 cttgccgatt tcttgttcgc ttactggctt tccttgcata aacttttcga gcttttgtat
    15541 atcgtcgtag ctataaaaag ttatacattt tccttcgcca ccgtctctgc cagctctacc
    15601 agtttcttga taatagcctt ctaagctttt tggaatattg tagtgaataa caaatctaac
    15661 atctggcttg tctattccca ttccaaaagc tatggttgca acaataacat ttgcttcttc
    15721 gttcaaaaac atatcttgat tttgtgaacg cacagcagcg tccattcccg catgatatgc
    15781 caaagcttta atgtcgttaa caactaacag ttctgcaagt tcttcaactt tttttcgact
    15841 taaacaataa ataattcctg attttccggg attatttttt atatatttaa taatttcttt
    15901 tgaagcattt atttttggtc taacttcata atataaatta tgtcggcgga aagaagcttt
    15961 aaaaacttta gcatctagca tgtcaaggtt tttttgaata tcatgttgaa ccttaggcgt
    16021 tgctgttgca gttaaagcca ttaaaggagt tcttcctatt gcatcaacta taggtctaat
    16081 ccttctgtat tctggtctaa agtcgtgtcc ccattcgcta atacaatgtg cttcatcaat
    16141 agcaaagaaa gatattttga actttttcat aaactcaaca ttctcttctt tggtaagaga
    16201 ttcaggagct acgtataaaa gttttgtttt accacttaaa acatcttctt ttacaatatt
    16261 tgtttcagtt ttatttaatg atgagtttag aaaatgtgca acgccactgt ctaaactaaa
    16321 tccacgcata gcatcaactt gatttttcat caatgcaatt aatggcgaaa taacaattgc
    16381 tgtgccttct aatattaatg ctggtaattg gtaacataaa gatttaccac cacctgttgg
    16441 cattaataca aaagaatcat gtccatcaag taggcttaaa attacttctt tttgttgacc
    16501 tttaaaattg tcgaatccaa aatatttctt taaaacttca aaaatttgtt tttctttatc
    16561 tgtcatttaa aatctctttt actataatta atatatattg tatattaaaa acattaatct
    16621 aaaaatcaat tattttagtc aaagttaaaa attaaaattt aataagtata tgtttttaaa
    16681 cttatttaac taaattttta atattttaat taagtttcat attaaaataa gtactaaatt
    16741 agcaaaaaaa atcgaatgca aaaaaataat attgaaaaaa ttatagaaat tgctaaaaaa
    16801 actattgttt tagaagcaga gtcaatttct aatttggtta attatattga caaaagtttt
    16861 gcagaagtag taaataaaat ttttgaatct actggcagag ttataattac aggtattggt
    16921 aagagtgcaa ttattggtaa taaaattgtt gcaagtttaa attcgacagg tacaccagca
    16981 atatttatgc acgcagcaga tgctatacat ggtgatttag gaattgtaca aaaagatgat
    17041 gttgtaattt gtttgtcgaa aagtggaaat acaccagaaa taaaagtttt gattccatta
    17101 ataaaaaata atggtaattt attagtggca atagttggtg atgaaaattc ttttttagct
    17161 aaaagtgccg attatgttct attaacaaca gtcgatagcg aggcttgtcc aaataattta
    17221 gcacctacat caagtactac tgctcagttg gttatgggcg atgctttaat gttggctttg
    17281 ttgcaaatgc gaggttttac agcaaaagat tttgcaagat ttcacccagg aggttcttta
    17341 ggcaaaaaat tatatttgcg tgtagatgat atttatattc aaaatgctaa accaaaagta
    17401 aaacctaatg ataatattac aaaagtaatt tctgaaatta caggcaaaag acttggagca
    17461 actgctgttg ttgatgaaaa tgatatgatg ttaggtataa ttactgatgg agatttaaga
    17521 agaatgttag aaaaatatcc ttctgagttt attaatttaa aagctaaaga tataatgtct
    17581 gataaaccca aaatggttga aaaagactgc cttgcaataa acgccctttc ggttattaaa
    17641 gcaaataata taagccaagt tgttgtggtc gaagacaata aatatttggg aattgtgcat
    17701 atgcacgatt tgatg
//
LOCUS       DAOPNX010000051        17347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_3918_length_17347_cov_7.160305, whole genome shotgun sequence.
ACCESSION   DAOPNX010000051 DAOPNX010000000
VERSION     DAOPNX010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17347
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3918_length_17347_cov_7.160305"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..569)
                     /locus_tag="PKN53_06900"
     CDS             complement(63..569)
                     /locus_tag="PKN53_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88712.1"
                     /translation="MKKVFLISIFCIFISFTSVSQEASGNVDFVIPTYSGADPSLGVG
                     LLYLYDLDNGINIGGYTAYRQMLNFKGYFSNIPIMFIARYYINGGSSGLYPQIGLGAD
                     YSIAVIKYKWWENLVYKVSSSAISFAYSVGLGGKLKNNLDLSGHFEGRVYKNNHRGYV
                     VLRVGYWF"
     gene            complement(625..1119)
                     /locus_tag="PKN53_06905"
     CDS             complement(625..1119)
                     /locus_tag="PKN53_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88713.1"
                     /translation="MKKAFLILIFCVFASFASKAQFSGFVDLKIPTAENSVPFFGPGV
                     QYLHELENGLSIGGLVAYRQRLDFNSFSGNIPIMLVSRYYTSGRGRGFYPQVGVGFNT
                     TFRIYKNKLLNRTDSYSITCFSYVAGIGGKFKNNLDISAHFEGYVLNAKHSGAILLRV
                     GFGI"
     gene            complement(1366..1944)
                     /locus_tag="PKN53_06910"
     CDS             complement(1366..1944)
                     /locus_tag="PKN53_06910"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HOD88714.1"
                     /translation="MRIFQIQIALKGFRPKIWRRILVPENVLLPDFHKIIQTTMGWTN
                     SHLHHFVKGRSYYVHKTEDDVFVGNNSDIDYAKIKISDLLSFEKDKIIYEYDFGDDWE
                     HDIVLEKILPYDNKLVYPICVKGKMNCPTEDCGGVLGYAYMLEVIKDPEHEDYEELRD
                     WLGEEFDPEFFDIDTVNELLKRKDFGCLDLFY"
     gene            complement(2135..2269)
                     /locus_tag="PKN53_06915"
     CDS             complement(2135..2269)
                     /locus_tag="PKN53_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88715.1"
                     /translation="MNPTLLSQYVQGHKKPSESQTEKIILGIQQIGQELSEINLIYKM
                     "
     gene            2394..2708
                     /locus_tag="PKN53_06920"
     CDS             2394..2708
                     /locus_tag="PKN53_06920"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOD88716.1"
                     /translation="MKKRHFTLEQKLAILKEASENGVSLTLKKHGVFPATYYQWKKKF
                     ETMGVEGFSHGMTPGQLKRIRELEKENQLLKEIIVEKELEGKLKDELLKKKFALEKRK
                     NS"
     gene            2762..3814
                     /locus_tag="PKN53_06925"
     CDS             2762..3814
                     /locus_tag="PKN53_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HOD88717.1"
                     /translation="MTKHQYYYKPKNGKRGRKKTTTVTTSCGVVDNDVVVSEIEKIQS
                     DPDTNYGYTKMTFELKTRGFYINKKKVYLLMKANGLLKAKNKTTPKNYAKYRKILPEK
                     PLEILEMDIKFVWIEEYKRHAAILTIIDTFTRYVLYYYVSFSIKKHDVKKCWEYVIIN
                     HLQGYVNPGEPIHIEVRNDNDKRFSAKLVQEYFAENFLNQVFTHPYTPQENGHIESFH
                     AILSEHLNRFNFWSIQELMQNLELFYEKYNNKRIHSSIAYLTPCDFWNLWDKNLIEKI
                     TNYKIRKTKFKLKIDYQEVKKHTGNNEPEVASLHNKSHLKGAPLIRQKEKVGADSTLN
                     IRSKKSPSVVHCYANI"
     gene            4153..5712
                     /locus_tag="PKN53_06930"
     CDS             4153..5712
                     /locus_tag="PKN53_06930"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88718.1"
                     /translation="MKIFLNILFFLIIGFSVFCQNDEYEFTRYFYPNGNISAEGYIRE
                     GKPDGYWKNYYEDGTLKSEGNRLFFELDSIWNFYYPDGLIENSINYRKDKKNGYSLHY
                     KYFYDEDSVKHHYLFSKELFLNGLREGTSFYYSTDGKLQYIFSYKADKKDGDAKEFNK
                     DSTVITLFKYYNGYEVEAKKINRTDAQKRKQGTWIEFYTNGNKKTEYNYINDVLHGYH
                     REYDVDGKMLTEQRYVNGELYVPELAQEEEIVLKAEIKKAYYPDGKIKFEGAFLEDKP
                     VGIHKEYDEQGHVKIAKEYTSEGEFLGQGLFDNNGKRTGLWKLYDDFNSYYFGEGNYV
                     DGKQDGKWIFYYKNGNVELEGYYSEGKPDREWIWTYENGATKRKENFIYGKREGEYAE
                     YDSVGNLILAGQYFDDARKGEWIIYVGEIVQRGNYNLDEKTGIWKYHYIDNNKIRFVG
                     NFKNNEPDGIHKWYYPNGNIEIYGTYRMGKKNKDWKKYNPDGSLLTTYTYRNDRLIKI
                     DGVKLYRGRKK"
     gene            complement(5757..6143)
                     /locus_tag="PKN53_06935"
     CDS             complement(5757..6143)
                     /locus_tag="PKN53_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOD88719.1"
                     /translation="MSKEEEKTRYSYEELMEFKEIILKKLEKANRDYELYKSMLSHED
                     TNDIMDTSPTFKVLEEGANVLSKEEIGKIAQRQLKFIQHLQAALARIETGTYGVCRET
                     GKLISKERLRAVPHATLSMDAKMNRK"
     gene            complement(6162..9590)
                     /gene="ileS"
                     /locus_tag="PKN53_06940"
     CDS             complement(6162..9590)
                     /gene="ileS"
                     /locus_tag="PKN53_06940"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOD88720.1"
                     /translation="MNKKFAEYEQLDLAAINAEMQELWKKENTFEQSVETRKDNKPFI
                     FFEGPPSANGTPGIHHVMARAIKDVVCRYKTLTGYRVDRKAGWDTHGLPVELSVEQTL
                     GITKEDIGKKISIKEYNNTCRKEVMKYKDLWEKLTEQMGYWVDMDNPYITCDNRYIET
                     VWYLLKDLFDKNLLYKGYTIQPYSPAAGTGLSTHELNQPGCYKDVKDTTVVALFSVIR
                     NEISEKLYKNANPNLYILAWTTTPWTLPSNTALAVGPEIKYVKINTFNPYSGNPISLI
                     VCKDRISAYFKADMQNADFEAYKPGDKNIPWQIIEEYSGKDLVGVSYEQLIDWVKTKS
                     PAFKVIAGDYVTTEDGTGIVHIAPTFGADDARVAKAAGIPALILNDKDGNICPMVDKQ
                     GKFFRIEDLHPDFVKNYVNVETYKDFAGRFVKNEYDENANPDISTDVDIAVMLKLQDK
                     AFKIEKYVHNYPHCWRTDKPILYYPLDSWFVKTTELKDRMIELNKTINWKPVSTGEGR
                     FGKWLENLVDWNLSRSRYWGTPLPIWSTEDGSERKCMGSVKELMDEIAISVEKGFMSK
                     DFWKGFVPGDYSKENYKKIDLHRPDVDEIVLVSSKGEKMFREPDLIDVWFDSGSMPYA
                     QWHWPFENKEEFPNLYPADFIAEGVDQTRGWFFTLHAISTMVSDSVAFKNIISNGLVL
                     DKDGNKMSKRLGNAVDPFSAMDEYGADALRWYMITNSQAWDNLKFDISGVDEVRRKFF
                     GTIYNTYNFFALYANIDNFRNLDKEIEFEKRPELDKWILSLLNSLIKDVGNAYENYEN
                     TRAGRLIQDFVIDHLSNWYVRLGRKRYWGGEYDEDKISAYQTLYTCLLTVSKLASPIA
                     PFYMDKLYRDLISVNDKEKVSSVHLAYFPEVNEEIIDKELEERMEMAQSISSMILSLR
                     RKANFKVRQPLQKIMMPIISDNFVEQIEKVEQIIKTETNIKEIKFLSDSSDILVKSIK
                     ANFKVLGPKYGKLMKQVAAKIAGMSQKEIAEFERTNKFSFVVENQEVNLDLDDVEIIS
                     EDIPGWLVANEGKITVALDINLTQELIEEGIAREFVNRIQNLRKESGFNVVDKINIYI
                     LSNPNLNSAITKFDEYICSQTLANSIKIVDKMEQKDNISVEVNNFETQLSVTKV"
     gene            9789..10610
                     /locus_tag="PKN53_06945"
     CDS             9789..10610
                     /locus_tag="PKN53_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88721.1"
                     /translation="MNNKSISKQSNAILTYFNEQDIKCFDYEQAKKALPKSKDSSLRE
                     LLRDMTRRGLLMRIKRGIYYIIPYEENPENFMPDWHLLAEYLVNGANYYIGYYSALQI
                     HNLIVQPSLKEQIVVSEQVKPSTLNIKGVDFQFIYHNKQHFFGAKKIWIDNYNKVICS
                     DLEKTFVDCLFKPDYAGGIVEIARAIYIVREKINYDILLDYVIKFNSQAIIKRLGFLL
                     EILEIKTAIIDDLQKLRTDSIIILDTELPKSGKITTKWSIQQNIETETIKTAIYT"
     gene            10652..11407
                     /locus_tag="PKN53_06950"
     CDS             10652..11407
                     /locus_tag="PKN53_06950"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOD88722.1"
                     /translation="MRDQQIEKDYVLSWILQGIVTHKVLSKLIVFKGGTVLKKVYFEN
                     YRFSEDLDFTLLDNSISNQQIFQYFKEVFDYIKEETNIPLEFADYNDLREINFYIKYV
                     GPLGGIGANKQVKVDISKNKKLLFAPEFKEVIIPYSDQGKHKLLCYSLEEILVEKLRS
                     VMQRMQARDFFDIWYLLEIQKLDLNFCKNEFIAKCKNKNINPFDFSNKLKLRLPQYKA
                     RWQKSMKDQIKDLPDFEQVEREVMRHLKNLDIQ"
     gene            11769..12530
                     /locus_tag="PKN53_06955"
     CDS             11769..12530
                     /locus_tag="PKN53_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88723.1"
                     /translation="MKKIFFLFFILITSLCFSQQAIRGYHVSYAYGYLSYADNFEINY
                     EDSSYLATPRNAIGDNISFGFPFDFGYKRHRLTLTPGLEISMANYNLNIDPDIPVFGN
                     NSDSLKLSSLMINPFVGAMYKLHFYVFKFHLALSIGADFKYTVSNTIIVNDHNGSQFL
                     TYPSLDNKTNDKIIFPQNSIFSSLSNIGFQICPKFGFDIYPTKFLVTHLFVSFSPLTS
                     YSKTPELRGYAGFGLSYLVPFKREDESKILQYYKK"
     gene            12539..15571
                     /locus_tag="PKN53_06960"
     CDS             12539..15571
                     /locus_tag="PKN53_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOD88724.1"
                     /translation="MKKSIYIFLLLLLNTFYLSSQTIIPVVQTGHTGSVLFVEWDPTS
                     RYVASIDSNNELVINDLISGKVFYSTKIQAQDDLVALNFKNERDLYIYGQSLVYHFDM
                     LTLKLELNISFEQYSMKTRTDKLKMKGSVVRKGMSKLYNRDAYTDFTYAITSNDENII
                     VAGDEKGGLYFCNNKLKVKKFTKSHFARINHIAFSPNNKYVAVASADRSISVWQTPSY
                     DLEKRIIPRSFNISSLESFSNDNLIAFGDELGFVYLLKFEEDKVSCEGINAHNGKIND
                     ICFSPNSNIIGTAGGDNRAAIVDFENKKVLQYFILHPKSTKAKQTFNIKGIQQRASSD
                     DKTRKNWVDENVYSVAISPDGKNIAYSGGKWGLSNPVLKVSSISGLKINLPKEQRKSK
                     RTNLGLGSKDNKHIFHQIIFTDNKDFIGLGEEQDRGVKFNMNITGYKQQESNNTSNLL
                     NKENPNNLLITEIIKEKSADNYIVKKDYSNGDVYTCNGYEIIRKHNGKEITFKGHRGP
                     INDFQILKGKDYLISAAEDASMNVWDLSTGEILLSIYVVDMGKLIFITPDNYYMATGD
                     ALTGMGFNFNGKVFPAEQFDLKFNRPDYILESFGYFDEDIIDVYHKAYQKRLAKLGFT
                     EKMLDGKIDLPEIKIANAANISLKTTQKTCKLNLEMLDHNYVLDRLNIYVNDVPIYGT
                     KGLSLLEENSKSIIKEVEIPLSDGDNYIYISCLNSKGVESLKQEVKIFYDKANKEKPD
                     LYLISLAVSEYEQSEYNLRYTINDGDGFMKLFENSKSFNNVNVYSLHNKECTRDNVLA
                     LKDKLMKSKVDDYVYVHIAGHGLLDDELDFYFATVDIDFDDPAKNGLKYDEIEGILDG
                     IPARNKLLLMDACHSGEVDKDDDFATDDKNSEDDTRGVTMFTNKNKNKPKTGLRNSFE
                     LMRLLFADLKKGTGTVVISAASGSGYALEIEKIENGIFTYCLINGIQKGKADTNKDKI
                     ISVSELRNYIFNGVKELSNGKQQPTSRQENLMNDFRVW"
     gene            16150..17145
                     /gene="nadA"
                     /locus_tag="PKN53_06965"
     CDS             16150..17145
                     /gene="nadA"
                     /locus_tag="PKN53_06965"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOD88725.1"
                     /translation="MNKSDLENFGFLKIDIPKDINLVEEIQKLKKEKNAVILGHYYIE
                     GDLQDVSDYVGDSLQLSQQAAKTDADIIVFLGVHFMAETAKILSPNKKVIIPDLKASC
                     SLAESCPENDFAKFIQDNPGHTVISYVNTTAAIKALTDIVVTSSNAKQIVDSLPKDEK
                     IIFGPDKNLGNYINSITGRNMLLWDGACHVHKQFDLEAILKIKSENPDAKIIAHPECE
                     SPVLVVSEHIGSTSSLLKFTQTDTSKKFIVATESGILHQMIKASPDKIFIPAPALDST
                     CACNDCNFMKLNTMEKLYNCLKYELPEITINEQLRQKAEKSIIKMLEISEKFGIK"
BASE COUNT         6399 a         2476 c         2529 g         5943 t
ORIGIN      
        1 gtcgaagcga ctttacaaac tttacaaact ttacgaactt tataaacttt taactgatat
       61 tcttaaaacc aatatcccac tctcaatacg acatatcctc tatgattgtt tttatataca
      121 cgcccttcga aatgtccgga taaatctaaa ttgtttttta attttccacc taagcctact
      181 gaatatgcaa aagatatagc tgaagaggaa actttgtaaa caagattttc ccaccatttg
      241 tatttaatta ctgcaatcga atagtctgca cctaaaccta tttgaggata aagtccacta
      301 cttccaccat ttatataata tctagcaata aacattattg gaatattaga aaaatatcct
      361 ttaaaattca acatttgacg ataagcagta tatccaccaa tgttaatgcc attgtctaag
      421 tcataaagat acaataatcc tactcctaaa gaaggatctg cgccggaata tgtaggaatt
      481 acaaaatcta catttccgct cgcttcttgg gacacagatg taaaacttat aaaaatacaa
      541 aaaatagaaa ttaaaaatac ttttttcata tataaaattt caaatattta atacatactt
      601 ttattaaaac aggtcttttt tttatcaaat tccaaatcca acccttaaca aaatagctcc
      661 actatgtttt gcatttagaa catatccttc gaaatgtgca gatatatcta aattgttttt
      721 gaattttccg cctataccag ctacatacga aaaacatgtt atcgaatatg agtctgttcg
      781 gtttagtaat ttatttttat atattctaaa tgttgtgtta aaacctacac ctacttgagg
      841 ataaaatcct ctgcccctac cgctagtata atatctagaa acaagcataa taggaatatt
      901 accactaaaa ctgttaaaat ctaatctttg gcgataagca actaatccac ctatactcaa
      961 accattttct aattcatgaa gatactgcac accaggacca aaaaaaggaa cagaattttc
     1021 ggctgtcggt atttttaaat caacaaaccc actaaactga gctttagatg caaaacttgc
     1081 aaaaacacaa aatatcaaaa ttaaaaatgc ttttttcata ctcataataa ttttcaataa
     1141 atacaaatat agtctttttt aaatataaaa aaatttattt gagtttttta ttataattta
     1201 gattcaatta gtaaatgcaa cattttcatt tcacaaatat agtaaaataa tttttagatt
     1261 ttaaattata gattaggttt ttaggtggtt aaaaataaaa gctaagcttt atatgaaaag
     1321 tcattcaaac gtaaaattct gtattacaaa tataagtagt tattgttaat aaaataaatc
     1381 taaacaccca aaatcttttc tttttaataa ttcatttaca gtgtcaatgt caaaaaattc
     1441 tggatcaaat tcttctccta accaatctct aagctcttca taatcttcat gttctggatc
     1501 ttttatcacc tctaacatgt atgcataccc gagaactcct ccgcagtctt cggttggaca
     1561 attcattttg ccttttacac aaatcggata aactaattta ttatcatacg gaagaatttt
     1621 ttctaacaca atatcatgct cccagtcatc accaaaatca tattcataaa taattttatc
     1681 cttttcgaaa ctaagtaagt ctgaaatttt aatttttgca taatcaatat cactattatt
     1741 acccacaaaa acatcatctt ctgtcttatg tacataatat gatctacctt tgacaaaatg
     1801 gtgcaaatga gaatttgtcc aacccattgt tgtttgaata attttatgaa aatcgggtaa
     1861 taatacattt tcaggaacta atattcttct ccagattttt ggtctaaacc cttttaaagc
     1921 aatttgaatt tgaaaaattc tcatatatac tcctttcttt taataataat taaaatagtt
     1981 ttgtgttgtg aaaataattg cttacaacct atttttccac accaaatttt tacaaatata
     2041 caagatatta ttttataaaa ggaagatttt ggggagaaat tttgggtggg gaaagtgaat
     2101 ttaaaaatgc aattgcactt atgggcatta ttatttacat tttatatatc agatttattt
     2161 ctgacagttc ttgtccaatt tgttggattc ctaaaattat tttctctgtt tgactttcgg
     2221 aaggtttttt atgtccttgc acatattgcg atagcaatgt tgggttcatt ccaatttttt
     2281 ctgctaaaaa ctttgcgtta ataactttat agtatttaaa gaattgtact attttagccc
     2341 cctaattcat cggacaaaat tatagtttaa aaaatataat taattttgtt tttatgaaga
     2401 aaagacattt tacattagag caaaaattgg ctattttaaa agaagcctct gaaaatggag
     2461 taagtttaac tcttaaaaag catggtgttt ttcctgcgac atactatcaa tggaaaaaaa
     2521 agtttgagac catgggtgtt gaaggtttta gtcatggcat gactccagga cagcttaagc
     2581 gcatacgtga attagaaaaa gagaatcaat tattaaaaga gattatcgtt gagaaagagc
     2641 ttgaaggcaa gcttaaagat gaacttttaa aaaagaagtt tgccttggag aaaagaaaga
     2701 actcgtagct aagtttattc ttcaaggctt aacaagagat aatgctctaa aaatagctaa
     2761 tatgacaaaa caccagtatt attataagcc taaaaatggc aaaagaggtc gtaagaaaac
     2821 gactactgta acaacttctt gtggagttgt tgacaatgat gttgtggttt cagaaataga
     2881 aaaaatacaa tccgacccag atacaaacta cggttacacc aaaatgacat ttgagttgaa
     2941 aacaaggggt ttttatataa ataaaaagaa ggtttacttg ttaatgaaag ccaatggttt
     3001 gctaaaagct aaaaataaaa ctacaccaaa aaactatgct aaatatcgaa aaattttacc
     3061 agaaaaaccg cttgaaattc ttgaaatgga tattaagttt gtgtggattg aggaatacaa
     3121 aagacatgca gcaattttaa ctattattga cacttttaca agatacgttc tttactatta
     3181 tgtaagtttt tcaataaaaa aacatgatgt taaaaaatgc tgggagtatg ttattattaa
     3241 tcatctgcaa ggatatgtaa accctggaga accaatacac attgaagtaa gaaatgataa
     3301 cgacaaacga ttttctgcta aattagtaca agaatatttt gcggagaact tccttaatca
     3361 ggtttttaca catccttata cacctcaaga aaatggtcat atagaaagct ttcatgcaat
     3421 cctatcagaa catcttaaca gatttaattt ttggtcaata caggaactaa tgcaaaactt
     3481 agagctgttt tatgaaaaat acaataacaa aagaatacat tcctcaattg catatttaac
     3541 accatgcgac ttttggaatt tatgggataa aaatttaata gaaaaaataa caaattataa
     3601 aataagaaaa acaaagttca aactaaaaat tgattatcaa gaagtgaaaa aacatacggg
     3661 taataatgag ccagaggttg cttcattgca taataaatcg cacctgaaag gtgcaccttt
     3721 gataaggcaa aaagagaagg tcggcgccga ctcaactcta aacatacggt ctaaaaaatc
     3781 accgtcggtt gttcattgct atgcaaatat ataatttttt tttatatgtt tgcactgtgg
     3841 ttaataaatt gtccgaataa tagggggtca aaccaataat gcttctttta tgtgttcttc
     3901 tgtcatcata atattaaaca aacaatgcaa atataatact tatttttaca tttcataata
     3961 taccaaaata attatctgaa tcttcctatt ttaccttcca aattaatttt tctacgtact
     4021 cccaaaaata aagtttcatt ttccactaat aaattggtac aagttttaaa actcatacaa
     4081 gttttttcta tttatttaaa aattttgact ttataagcta attgctaagt aaatattaat
     4141 taaatttaca ccgtgaagat ttttttaaat atattattct ttttgattat tggctttagc
     4201 gttttttgcc aaaacgatga gtatgagttt acaagatatt tttatccaaa tggaaatata
     4261 tcggctgaag gttatattcg tgagggaaaa cctgatggtt attggaaaaa ttattacgaa
     4321 gacggtactc taaaatctga aggaaatcga ctgttttttg aattggacag catttggaat
     4381 ttttactacc ctgatggact tattgaaaac tctataaact atcgtaaaga caaaaaaaat
     4441 ggctactccc ttcactacaa gtatttttat gacgaagatt ctgttaaaca tcattacctg
     4501 ttttcaaaag aactttttct aaacggtctg cgtgaaggga cttcatttta ctactcaact
     4561 gatggaaaat tacaatatat attttcatat aaagccgata aaaaagatgg agacgctaaa
     4621 gaatttaaca aagacagcac agtaattact ctattcaaat attataacgg ctatgaggtt
     4681 gaagctaaaa aaataaatag aactgatgcc caaaaacgta agcaaggaac ttggattgag
     4741 ttttatacta acggaaataa aaaaaccgaa tacaactata taaacgatgt tttacacggc
     4801 taccataggg aatatgatgt tgatggtaaa atgctaacag aacaaagata tgtaaatggc
     4861 gaattgtacg ttccagaatt ggcacaagaa gaagaaattg ttttaaaagc tgaaattaaa
     4921 aaagcatact accccgatgg aaaaattaaa tttgaaggtg cttttttaga agataaaccc
     4981 gttggaattc ataaagaata tgacgagcaa ggacatgtaa aaatagcaaa agaatatact
     5041 tccgaaggcg aatttttggg tcaaggtttg ttcgataata acggtaaacg tacaggattg
     5101 tggaaattat atgatgattt taactcatat tattttggcg aaggaaatta tgttgacgga
     5161 aaacaagacg ggaaatggat tttttattat aaaaatggaa atgtcgaact tgaaggctat
     5221 tactctgaag gtaaaccaga tagagaatgg atttggactt acgaaaatgg tgctacaaaa
     5281 agaaaagaaa actttatcta cggaaaacgc gaaggcgaat atgccgaata cgactctgta
     5341 ggaaatttaa ttttagctgg tcagtatttt gacgatgcac gaaaaggcga atggataatt
     5401 tatgtgggag aaattgtaca aagaggaaat tataatttag acgaaaaaac cggcatttgg
     5461 aaatatcatt atatcgacaa taataaaata agatttgtag gaaattttaa aaataacgaa
     5521 ccagacggca tccacaaatg gtattatcct aatggaaata tcgaaatata cggtacttat
     5581 cgcatgggta agaaaaataa agattggaaa aaatacaatc ctgacggctc actgcttact
     5641 acctacacat acagaaatga tagactaatc aaaatagacg gagtaaaact ttatagagga
     5701 agaaaaaagt aaaaaactaa ggctaacagc tatgattttt tgttaaaacc ataattttat
     5761 tttctattca tttttgcatc catgctaagt gttgcatgag gaactgctct aagtctttct
     5821 tttgagatta attttcctgt ttctctacaa acaccgtaag tacctgtttc aattcgcgca
     5881 agagctgctt gtaaatgttg aataaatttt aactgccttt gagcaatttt gccaatttct
     5941 tctttagata atacatttgc accttcttcc aaaactttga aagttggcga tgtatccatt
     6001 atatcgttag tgtcttcgtg acttaacatg cttttatata attcgtaatc acgatttgct
     6061 ttttcaagtt ttttaagaat aatttcctta aattccatta gctcttcata gctgtatctg
     6121 gttttttctt cttctttact cataatcaaa ttttttaaac attaaacctt agttacactc
     6181 aattgcgttt caaaattatt tacttctacg gaaatattat ctttttgttc cattttatca
     6241 acaattttta tagaatttgc taaagtttgt gaacaaatat attcatcaaa cttagtaata
     6301 gcagagttta gattaggatt tgaaagaatg taaatattta ttttatcaac cacattaaat
     6361 ccactttctt ttcttaaatt ttgaattcta tttacaaatt ctcttgcaat tccttcttct
     6421 attaattctt gtgttaaatt tatgtcgagt gctacagtaa tttttccttc gttagcaacc
     6481 aaccaacctg gaatatcttc agaaataatt tcaacatcat ctaagtcaag attaacttct
     6541 tgattttcaa caacaaaact aaatttattt gtgcgttcaa attctgcaat ttctttttga
     6601 gacattcctg caatttttgc agcaacttgt ttcatcaatt taccgtattt tggtcctaaa
     6661 accttaaaat tagctttaat actcttaacc aaaatatcag aactatcaga taagaatttt
     6721 atttctttaa tatttgtttc agttttaata atttgctcta ctttttcgat ttgctcaaca
     6781 aagttatcag aaataatagg catcataatt ttttgaagag gttgtctaac cttaaaatta
     6841 gcttttcgtc ttaaacttaa aatcattgac gaaatagact gtgccatttc cattctttct
     6901 tcaagttctt tgtcaataat ttcttcgttt acttctggga aataagctaa atgcaccgag
     6961 cttacttttt ctttatcgtt aaccgaaatt aaatctctat aaagtttatc catataaaat
     7021 ggtgctattg gcgatgctaa ttttgaaact gttagcaaac aagtatataa agtttgataa
     7081 gccgaaattt tatcttcatc gtattctcca ccccaatagc gttttcgtcc aagtcttaca
     7141 taccagttgc ttaggtggtc aattacaaaa tcttgtatta aacgtcctgc tcttgtgttt
     7201 tcgtagtttt cgtaagcatt tcccacatct ttaattaaag aatttagcaa gctcaaaatc
     7261 catttatcaa gctctggacg tttttcaaat tctatttctt tatctaaatt tctaaagtta
     7321 tcaatatttg cataaagagc aaagaagttg taagtattgt aaatagttcc aaaaaattta
     7381 cgtcttacct catcaactcc agaaatatca aattttaaat tatcccaagc ctgagaattt
     7441 gtaatcatat accatcttag cgcatctgca ccgtattcgt ccattgccga aaatgggtca
     7501 acagcatttc cgaggcgttt agacattttg tttccgtctt tgtcgaggac taagccattt
     7561 gaaataatat ttttaaaagc taccgagtcg gaaaccattg ttgaaattgc gtgtaaagta
     7621 aaaaaccatc cgcgagtttg gtcaacacct tcggcaataa aatcggcagg ataaagattt
     7681 ggaaattctt ctttattctc gaagggccaa tgccattgtg cataaggcat agagccagaa
     7741 tcaaaccaaa cgtcaattag gtcgggttcg cgaaacattt tttcaccttt agaagaaact
     7801 aaaacaattt catcaacatc aggtcgatgc aaatctattt ttttatagtt ttcttttgaa
     7861 taatcaccgg gaacaaagcc tttccaaaaa tctttagaca taaaaccttt ttcaacagaa
     7921 atagcaattt cgtccattaa ttctttaaca cttcccatac attttctttc gcttccatct
     7981 tctgtactcc agataggcaa aggtgttccc caatatctcg aacgactaag attccagtca
     8041 accaaatttt ctaaccattt tccaaatcta ccttctccag tacttacagg tttccaattt
     8101 atggttttat ttagttcaat cattctgtct ttaagttctg tagttttaac aaaccaagaa
     8161 tctaaaggat aatacaaaat tggtttatct gttctccagc aatgaggata attgtgaaca
     8221 tatttttcaa ttttgaaagc tttgtcctgc agttttaaca ttacagcaat atcaacatcg
     8281 gttgaaatgt cgggatttgc attttcgtcg tattcatttt taacaaatct gccagcaaaa
     8341 tctttatatg tttcaacatt tacatagttt ttaacaaaat ctggatgtaa atcttctatt
     8401 ctaaagaatt ttccttgttt atcaaccatt ggacaaatat ttccgtcttt atcgttgagt
     8461 ataagtgcgg gaatacctgc tgcttttgca acgcgagcgt catcagcacc aaaagttgga
     8521 gcaatatgca ctataccagt tccgtcttcg gtagttacgt agtcgcctgc aattacttta
     8581 aaagctggtg attttgtttt tacccaatct atcaattgtt cgtaagaaac tccaaccaaa
     8641 tctttgccag aatattcttc gataatttgc caaggaatat ttttatcacc gggtttatat
     8701 gcttcaaaat cagcattttg catatcggct ttaaaatatg cagaaattct atctttacat
     8761 acaataagcg aaattggatt tccagaataa ggattaaaag tatttatttt tacatattta
     8821 atttctggac caacagcaag agcggtgttt gaaggaagag tccaaggtgt ggtagtccaa
     8881 gctaaaatat aaagatttgg atttgcattt ttataaagtt tttcagagat ttcatttcta
     8941 atcacagaaa aaagagcaac tacagtagtg tctttcacat ctttataaca accaggttga
     9001 ttaagttcgt gagtacttaa acctgttccg gctgctggtg aataaggttg aatagtatag
     9061 cctttgtaaa gtaaattttt atcaaataaa tctttaagca aataccaaac tgtttcaata
     9121 tatcggttgt cgcaagtgat ataaggattg tccatatcaa cccaatatcc catttgttct
     9181 gtaagttttt cccacaaatc tttatatttc atcacctctt tgcgacaggt attattatat
     9241 tctttaatag aaattttctt gcctatatct tctttagtaa ttcccaaagt ttgttctacg
     9301 ctaagctcta caggcaaacc gtgagtatcc caacctgctt tacggtcaac gcgatagcca
     9361 gttaatgttt tgtaacggca aaccacatct tttatagcac gagccataac atgatgaatg
     9421 ccaggagttc catttgcaga aggcggacct tcaaaaaata taaaaggttt attatcttta
     9481 cgtgtttcta cactttgttc aaaagtattt tcttttttcc acaactcttg catttcagca
     9541 ttaattgctg ctaggtcaag ttgctcatat tcagcaaatt tcttattcat ctatctataa
     9601 actttaaagt tgcaaatata tatttctaga ccaaaatatg caaatcttaa aaatgatttg
     9661 ttttttaatg gtttttataa atgtgtaatg tggatttttt tgtatttttg agaaataaat
     9721 aaaaaataat tttattattt aattatgttt tatatctttg taggcagttt taagctgaca
     9781 aatagactat gaacaataag tcaatatcaa aacaatctaa tgcaatcctc acatatttta
     9841 atgagcagga cataaaatgt tttgattatg aacaagcaaa aaaagcttta ccaaaatcca
     9901 aagacagtag tttgagagag ttgctcagag atatgacacg cagaggattg ttaatgagaa
     9961 taaaacgagg tatatattac attattccgt atgaggaaaa ccctgagaat tttatgcctg
    10021 attggcacct tcttgcggag tatttagtaa atggagcaaa ttattatatt gggtattatt
    10081 ccgctttaca aatacataat ttaattgttc agccttcatt gaaagaacaa attgttgttt
    10141 cagaacaagt aaaaccctca actttaaata ttaaaggtgt agattttcaa tttatttatc
    10201 ataataaaca acattttttt ggtgcaaaaa aaatatggat agacaactat aataaagtga
    10261 tttgttctga cttagaaaaa acatttgttg attgtttatt taagcctgat tatgctggtg
    10321 gtattgtaga aatagcaagg gcaatttata ttgtacgaga aaaaattaat tatgatatct
    10381 tgctagatta tgttattaaa tttaattcac aagccattat aaaacgatta ggctttttat
    10441 tggagatatt agaaataaaa actgccataa ttgacgactt gcaaaaacta agaacagatt
    10501 ccattataat tttagataca gaactaccaa aatcaggaaa aataacaaca aaatggagta
    10561 ttcagcaaaa tattgaaaca gaaactataa aaacagcaat atacacatga taaaaccagg
    10621 agaaatataa aagattgcac gaaaagttgg tgtgcgtgac caacaaatag agaaagatta
    10681 tgtcttatct tggattttgc aaggtattgt cacgcacaaa gttctttcaa aattaatagt
    10741 ttttaaagga ggaactgtat taaaaaaagt ttattttgaa aattacagat tttcagaaga
    10801 tcttgatttt acacttcttg acaatagtat ttctaatcaa caaatatttc aatattttaa
    10861 agaagtgttt gattatatca aagaggaaac aaatattcca ttagaatttg ctgattataa
    10921 tgatcttaga gaaataaatt tttacatcaa atatgttgga ccattaggtg gtatcggagc
    10981 aaataaacaa gtaaaagttg atatttctaa aaataaaaaa ctattatttg ctcctgagtt
    11041 taaagaagtt attattcctt attctgatca gggaaaacat aaattgcttt gttactcact
    11101 tgaagaaatc cttgtggaaa aattgcgctc tgtaatgcaa agaatgcaag caagagattt
    11161 tttcgacata tggtatttgt tagaaattca aaagttagac cttaattttt gtaaaaatga
    11221 atttattgca aaatgtaaaa ataaaaatat taaccctttt gatttttcaa ataaattgaa
    11281 actacgcttg ccacaatata aagccagatg gcaaaaatca atgaaagacc aaattaaaga
    11341 cttgccagat tttgaacaag tggagcgaga agtaatgaga catttgaaaa acttagatat
    11401 acaataatct tttgtatttt tgaggaataa aattaaaaat aatttaatta cgttttatat
    11461 ccccaaaaac agttttaaac tgacaaacaa gatatgaata ataagttgtt ttaaaattat
    11521 ttaatacaat tttacctatt tgatcggatt tagcttcgct gagctcgctt ccaacaccca
    11581 gatgctcgct cggggttgtg cggggaaaat ttgcataagc tggattaagc aggaaagtct
    11641 gagagagcag ggaaaatatt tttttcttat tttgcttcaa tttactttaa tatcaaataa
    11701 atattatact tttgttgttt aaaatcttaa ttttattagt tttagatgtt taaattaaaa
    11761 tttagcatat gaaaaaaata ttttttttgt ttttcatttt aattacaagc ctttgttttt
    11821 cacaacaagc aattagaggc tatcatgttt cttatgctta tggttattta agttatgctg
    11881 ataattttga aataaattat gaagatagtt catatttagc aacaccacgc aacgccattg
    11941 gagataacat aagttttggt ttcccttttg attttggata taaacgacat agactaacat
    12001 taacgcctgg attagaaatt tcaatggcta attataattt gaatattgat ccagatattc
    12061 cagtttttgg taataatagc gatagtttaa aattatcgtc tttaatgata aatccatttg
    12121 taggtgcaat gtataaatta catttttatg tttttaaatt tcatttagct ttaagtattg
    12181 gtgcagattt taaatatact gttagcaaca caattattgt aaatgaccat aatggttcgc
    12241 aatttttaac atatccaagt ttagataaca aaactaatga taaaatcatt tttccacaaa
    12301 acagtatatt ttcaagtcta agtaatattg gttttcaaat atgcccaaaa tttggatttg
    12361 atatttaccc aactaaattt ttagtaactc atttatttgt gagtttttca ccattaacat
    12421 catactcaaa aacacctgag ttaagaggct atgctggttt tggtttaagt tatttagttc
    12481 cttttaaaag agaagatgag tcaaaaattt tacaatatta taaaaaataa taaacgatat
    12541 gaaaaagtca atttacatat ttttgctatt attgcttaat actttttatt taagctctca
    12601 aacaattatt cctgttgttc aaacaggaca tacaggcagt gttttatttg ttgaatggga
    12661 cccaacatct agatatgtag caagtattga ttctaataac gaattagtaa tcaacgattt
    12721 aatatcaggg aaagtatttt attcaacaaa aattcaagct caagatgatt tagttgcttt
    12781 aaactttaaa aacgaaagag atttatatat ttatggtcaa tcattagttt accacttcga
    12841 tatgttgacc ttgaaattag aacttaatat ttcttttgag cagtattcta tgaaaactag
    12901 aactgataaa ttaaaaatga aaggctctgt ggttagaaaa ggcatgtcta aactatataa
    12961 tcgtgatgca tatactgatt ttacttatgc aataacatct aacgatgaaa atattattgt
    13021 tgctggagac gaaaaaggcg gtttgtattt ttgtaataat aaattaaaag ttaaaaaatt
    13081 tactaaatct cactttgcaa gaattaacca tattgctttt tcacccaata ataaatatgt
    13141 agcagtagct tcagcagata ggtcaatatc agtttggcaa actccaagtt atgatttgga
    13201 aaaacgtata attccacgta gctttaatat ttcaagttta gaaagtttta gtaatgataa
    13261 tttaattgct tttggagacg agcttggttt tgtttattta ctaaaatttg aagaagataa
    13321 agttagttgc gaaggaatta atgcacataa tggaaaaata aacgatattt gtttttcacc
    13381 aaattcaaat attataggca ctgcaggagg cgataataga gcagcaatag tggattttga
    13441 aaataaaaaa gttttacaat attttatttt acaccctaaa tcaacaaaag caaaacaaac
    13501 tttcaatatt aaaggaatac agcaaagagc aagtagcgat gataaaacac gcaaaaattg
    13561 ggttgatgaa aatgtatatt cagttgcaat tagcccagat ggtaaaaaca ttgcctattc
    13621 tggcggaaaa tggggtttat caaatcctgt tttgaaagta tcatcaatat ctggtttaaa
    13681 aataaatttg cctaaagaac aaagaaaatc aaaaagaaca aatttgggtt taggctcaaa
    13741 agataataaa catatttttc atcaaataat ttttactgat aataaagatt ttatcggttt
    13801 aggtgaagag caagatcgtg gagttaaatt taatatgaat attactggat ataagcaaca
    13861 agaaagtaat aatactagta atttattaaa taaagaaaat cctaataatc tcttaattac
    13921 agaaattata aaagaaaaat cagcagataa ttatattgtt aaaaaagatt atagcaatgg
    13981 cgatgtttac acttgtaatg gatatgaaat tataagaaaa cataatggaa aagaaataac
    14041 ttttaaagga catagaggac ctataaatga ttttcaaata ttaaaaggca aagattattt
    14101 aattagtgct gctgaagatg cttctatgaa tgtatgggac ttatcaacag gagaaatttt
    14161 gctaagtatt tatgttgtgg atatgggtaa attaattttt attactcccg ataattacta
    14221 catggcaact ggcgatgcac taacaggaat gggatttaat tttaatggaa aagtttttcc
    14281 tgccgaacaa tttgacctta aatttaatcg cccagattat attttagaat cttttggata
    14341 ttttgatgaa gatattattg atgtttatca taaagcatat caaaaacgtt tagcaaaact
    14401 tggttttacc gaaaaaatgt tggacggcaa aattgattta ccagaaataa aaattgctaa
    14461 tgctgctaat attagcttaa aaacaactca aaaaacttgt aaattaaatt tagaaatgct
    14521 ggaccataat tatgttttag atagactaaa tatatatgta aacgatgtgc ctatttatgg
    14581 tacaaaagga ttatctttat tagaagaaaa ttcaaaatct ataattaaag aagtagaaat
    14641 tcctttatca gatggtgata attatattta tatttcatgc ctaaattcta aaggtgtaga
    14701 atctttaaaa caagaagtaa agatttttta tgataaagcc aataaagaaa aaccagattt
    14761 atatttaatt tccttagctg tttcagaata tgaacaatca gaatacaatt taagatatac
    14821 aataaatgac ggcgatggat ttatgaaact ttttgagaat agtaaatctt ttaataatgt
    14881 aaatgtttat tcacttcata ataaagaatg tacaagagat aatgtgttgg cattaaaaga
    14941 taaattaatg aaaagcaaag tagatgatta tgtttatgta cacattgcag gacacggtct
    15001 gctagatgat gaattagatt tttattttgc aactgttgat atagattttg acgatccagc
    15061 taaaaatggt ttaaaatatg atgaaataga aggcatttta gacggtattc cagcacgaaa
    15121 taaattatta ttaatggatg cttgtcactc tggcgaagtt gataaagatg atgattttgc
    15181 tacagacgat aaaaatagcg aagatgatac taggggtgta acaatgttta caaataaaaa
    15241 caaaaataag ccaaaaacag gattaagaaa ttcatttgaa ttaatgcgtt tattatttgc
    15301 tgatttgaaa aaaggaacag gaacagttgt tatttcagct gcttcaggaa gtggttacgc
    15361 tttagaaatc gaaaaaattg aaaacggtat ttttacatat tgcttaatta acggtattca
    15421 aaaaggaaaa gccgacacca ataaagacaa aattataagc gtttccgaat taagaaatta
    15481 tatttttaac ggtgttaaag aacttagcaa tggtaaacaa caacctactt cacgacaaga
    15541 aaatttaatg aatgatttta gagtttggta aattacaccg ctggctgttg tttacgaaca
    15601 aaccattaac tatcaaacat ttaccttctt taaaaacttt atgaacttta aaaactttca
    15661 acttacattt ttttatcaac aatattacaa acctgttaat ttactgttac ataaacaatt
    15721 aaaaaaccaa aaatttttaa atgcttgatt tacaatcttg taaaatatta acaacttata
    15781 taaaacaata aatattttgt cgataacttt taataaatta ttttttttaa ttcaaacaaa
    15841 acttagatat aaaaaatttt aaaaattcca aaatacttag cacaaatttt tacaaaataa
    15901 ttaacaattt tatacacgaa aattaaaaaa ataatactta aaaatgcttt taataaaaac
    15961 tattaacagt tgtgaataaa aaaattaaaa tattgaaaat caaagaaagt ttgaaaattt
    16021 cattgtttat aaaatttaat aagttatatt tgtaagcgaa ttaaaataaa tattaattat
    16081 ttttaaacaa tattaacaac taataataat aatcatattt taaattaatt aataaaaatt
    16141 aatattatta tgaataaatc agacttggaa aattttgggt ttttaaaaat tgatattcca
    16201 aaagacataa atttagttga ggaaattcaa aaattaaaaa aggaaaaaaa tgctgtaatt
    16261 ttggggcatt attacataga gggagattta caagatgttt ctgattatgt tggcgatagt
    16321 ttacaattgt cgcaacaagc agcaaaaact gatgcagata ttattgtttt tcttggagtt
    16381 cattttatgg ctgagactgc aaaaatttta tctccaaaca aaaaagttat aattcctgat
    16441 ttaaaagcta gttgctcttt ggctgagtct tgcccagaaa atgattttgc aaaatttata
    16501 caagataatc caggacatac agttatttct tatgtaaata ctactgccgc tataaaggct
    16561 ttaaccgata ttgttgttac ttcgtcaaat gctaaacaaa ttgtggatag tttaccaaaa
    16621 gacgaaaaaa ttatttttgg tccagataaa aatttaggaa attatataaa cagtattaca
    16681 ggtagaaata tgcttttgtg ggatggagct tgtcatgttc ataaacaatt tgatttagaa
    16741 gcaattttaa aaataaaatc agaaaatccg gatgccaaaa ttatagcaca tcctgaatgt
    16801 gaaagtcctg tacttgttgt ttcagaacat attggaagta catccagttt attaaagttt
    16861 acacaaacag atacatcaaa aaagtttatt gttgcaacag aatcaggtat tttgcaccaa
    16921 atgattaaag ctagtcctga taaaattttt attcctgctc ctgctcttga ttcaacttgt
    16981 gcttgtaatg attgtaattt tatgaaactt aatactatgg aaaaattata taattgttta
    17041 aaatatgaat tgcccgaaat tactataaat gagcagctta gacaaaaagc agaaaaatca
    17101 attataaaaa tgttagaaat ttctgaaaag tttggaataa aataactaag tttgcttttt
    17161 ataaatcaaa atttaagtat tatgaaaaaa atatttttta ttagtttatt tgtaagtctt
    17221 gcttttacaa gttttagcca gttttatgca ggagcacatt ttgcttataa tagcacttgg
    17281 ttgatgaata agcaagtttt tgatgaaggt ccagaaatgg atattgctgt ttcttttgga
    17341 aattatt
//
LOCUS       DAOPNX010000052        17171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_3972_length_17171_cov_7.612818, whole genome shotgun sequence.
ACCESSION   DAOPNX010000052 DAOPNX010000000
VERSION     DAOPNX010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17171
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3972_length_17171_cov_7.612818"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PKN53_06970"
     CDS             complement(<1..378)
                     /locus_tag="PKN53_06970"
                     /inference="COORDINATES: protein motif:HMM:NF025639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOD88726.1"
                     /translation="MKKIKKPNLFNIATKELSQDGFFTWLLQWGDPEYKKYNKKLFNC
                     SQNFIKFLLSKEFTKTINITKVVALRQQENIDIWVEINDKYLIIIEDKIFTGEHSSQL
                     KKYKTIATTWCKENNYKLVCIYLK"
     gene            complement(397..882)
                     /locus_tag="PKN53_06975"
     CDS             complement(397..882)
                     /locus_tag="PKN53_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88727.1"
                     /translation="MKDYKNFKSLLEYFVSHLEYCVTQDKNGRGYDTYIKNVKNFKKS
                     GYGDKGHKIQEQIKKWEDYENGKICFNVNATGYREWGCYLKWKDIASNVRGVWNNNEV
                     VKLQIYKNSTSKKKAIFIKDSEFSCQELGLFDGNPPNEKLKIFFDIFNDLIIEHNQRN
                     Q"
     gene            complement(1608..2543)
                     /locus_tag="PKN53_06980"
     CDS             complement(1608..2543)
                     /locus_tag="PKN53_06980"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014708477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HOD88728.1"
                     /translation="MSVLQTYKLRQIKSPLNYIGGKSKLLPQLLPLFPKHIDNFIDLF
                     AGGCNVGINVTANKIYCNDILSYLIEMYKVFQQNSMDNTLKYIEKRINDFNLSLTNET
                     GYKNLRKEYNEHKNPLDLFVLVAFSFNHQIRFNNSHEFNNPFGRERSSFNATMRQNLV
                     RFILRIKEINIEFSDFCFNDFDFSNFGINDFVYCDPPYLITTGTYNDGKRGFKGWTEK
                     EEEELLNLLDDLNGKNIKFALSNVLEHKGKSNKILKTWINNNPNYKINFIDYNYSNSN
                     YQTIVRDKNASIEVLITNYEPEKPIKEPTLFDNQV"
     gene            complement(2626..3183)
                     /gene="ruvC"
                     /locus_tag="PKN53_06985"
     CDS             complement(2626..3183)
                     /gene="ruvC"
                     /locus_tag="PKN53_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOD88729.1"
                     /translation="MEKVEKDRVILGIDPGTNIMGYGVIHEKGKTIECVGFGVVDMRK
                     LDGHFNKLKHIYERTIQLIDGFKPDELAIESPFQGKNVQSMLKLGRAQGAAISAALTR
                     TIPIYEYAPRKIKLAITGSGAASKEQVAGMLQKLLKLEELPKELDATDGLAAAMCHYY
                     QKEKITSDSKIKNWKDYVDRKNIEY"
     gene            complement(3184..4998)
                     /locus_tag="PKN53_06990"
     CDS             complement(3184..4998)
                     /locus_tag="PKN53_06990"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD88730.1"
                     /translation="MNFKLYKYFIFLIVFTVFSQFSAFSQNADAQLAFRYYQNKEFEK
                     AATLYEKLYRQNGYKNNRDYYLRCLSELKEFDTAEKFLKKEIKKNANDFYLMIDLGMV
                     YQQTNRFKDAIAEFDKVVEIVKLNRNNINAAAAVFTGYRQYEYAEKVYLEGVKNTNYD
                     YTMELGNLFYVQRDYPKMMSYYLDYLEEYPQNLNVIQSRLQYVMTNDIDQSIDDIVET
                     SVMDRIQKKPQVEVYSQLIIWQYTQTGRFRLALNQLFAIDKRNKKSESDILNYGIILF
                     ENLEYDLALEAFNYIMNKGTDNAYYSSAYVESLNVLYVKTLNQEKPSKDELTQLRNKL
                     EESLKVLPRKETYKIIYAIINLDAFYLNNYDSAINLAVKSIEDNRFITEQTLTLKLLL
                     ADIYFLSENTWEAILTYAEVEKAATERPIGHEARFRKAKLAYYTGQFKWAQAQLDVLK
                     ASTSKLIANDAMELSMFISDNYNLDTTETTMQIFARADFYIFSKQYERAFAGLDSIVE
                     LYPNHGLIDDVLYRKGEIYQAIGNYEKASSYYNQVATTYYYDILGDNALFKYAVCQEK
                     LRHFDTAQEAYLKLIKDYPGSIFTVEARKNLRRLSESK"
     gene            complement(5008..6282)
                     /gene="serS"
                     /locus_tag="PKN53_06995"
     CDS             complement(5008..6282)
                     /gene="serS"
                     /locus_tag="PKN53_06995"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOD88731.1"
                     /translation="MLTLRLIREEKDRVIKQLRVKNFEAEELIEKIIELDNKRKDYQF
                     NKDNLQSQLNDISKEIGILYAQKKKDEAEQAKGKTVGIKEELQKFEDLLSSVEKEMDS
                     IIVLLPNIPHESVPAGKTAEDNEIVKEGGKMPDLTVKKPHWDLIKEYDIIDFELGTKI
                     TGAGFPVYKGKGAKLQRALINFFLDQAEKAGYTEIQPPLLVNEDSGFGTGQLPDKEGQ
                     MYHITLDNFYLIPTAEVPVTNIYRNVILEEKDFPIKNVAYSACFRREAGSWGAHVRGL
                     NRLHQFDKVEIVQIAKPENSYAVLEEMKNHIESLLIKLNLPYRILKLCGGDISFTSAL
                     TFDFEVFSAAQERWLEVSSVSNFEDFQANRLKLRYRPENEKKTRLAHSLNGSALALPR
                     IMAALLENNQTEKGIVVPEVLRHFTGFDIITK"
     assembly_gap    6671..6770
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7037..9595)
                     /locus_tag="PKN53_07000"
     CDS             complement(7037..9595)
                     /locus_tag="PKN53_07000"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOD88732.1"
                     /translation="MTQNFKEEVKRKIFSDSEAFLASQEYFKGDDLAARVWINKYALK
                     DSDGNLYELTPDDMHRRLAKEIYRIETKYDNPLSEETIYELLKDFKYIVPQGGPMSGI
                     GNNLQIVSLSNCFVIGHDKPTDSYGGILKIDQEQVQLMKRRGGVGHDLSHLRPKGSPV
                     KNSALTSTGIVPFMERYSNTTREVAQDGRRGALMLSVSVKHPDSEDFIDVKLTQGKVT
                     GANVSVKLTDEFMNALINKDKFIQQFPIDDRAPKYIKEVDPQKIWKKIIHNAWKSAEP
                     GILFWDTIIRESVADCYADLGYKTVSTNPCGEIPLCPYDSCRLLAINLYSYVDNPFTK
                     EAKFNFDKFKKHVGYAQKMMDDIIDLELEKIDQILAKINSDPEDIEVKRVERKLWENI
                     RRKAEEGRRTGVGITAEGDMLAALGLTYGTDTANEFATEVHKTLAIEAYRSSVNMAKE
                     RMPFMIYDSQREKNNPFIERLRQADPEMVREMENYGRRNISLLTIAPTGTTSLMTQTT
                     SGIEPVFRPFYTRRRKVNPNDPEVRVDFVDEQGDKWEEYKVFHHKFLTWLQINKYDVE
                     EVKNMSNDQVEELVKKSPYYKATAADIDYLSKVRMQGAIQKWVDHSISVTINMPENVS
                     EEIVEECYFEAWKCGCKGITVYREGSRAGVLISDDKNNQQAKQMLELKRPKVLDADIV
                     RFRNDDEEWIAFVGLLNGMPYEIFTGRVEEDTLNIPKAIENGRIIKERDAEGNNRYDF
                     QYTNRYGYATTFQGLSHQFDKTFWNYAKLISGVLRHGMAIDKAVELVSSLEFDNDSIN
                     TWKRGVERALRKYIPNGTKASKKGDICEGCQQEDTQVYQDGCVICTECGHSKCS"
     gene            complement(10027..11172)
                     /locus_tag="PKN53_07005"
     CDS             complement(10027..11172)
                     /locus_tag="PKN53_07005"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOD88733.1"
                     /translation="MIEGRKNQIQEIKSKIDKNSKYIWFHCASLGEFEQGRPLIEKIK
                     TEKPEYKIILSFFSPSGYEPRKNYQFADIVCYLPFDSRKNAKEFIETVNPVTAIFVKY
                     EIWYYFLEKLNKNKIPTYLIAAIFRPNQVFFKSYGKTYRKALGFFSKIFVQTEETEKL
                     LHKYNFKNTLVCGDTRIDRVVAIANEKYENPILEKFCEGKKCFVYGSTWAADEKIITN
                     FINSSPEDYVFIIAPHEISEQNIKNLEKRLKITSQRLSKIETLNPDTRLIIIDSIGIL
                     SKLYRYANIAYIGGGFGKGIHNILEAVVYEIPVVFGPNHKKFNEAVQLIKNEVAFSIN
                     NSEEYKNIMNVLISDVNVIEKCKINANSYVNNSFGSTHKIFDNLHYL"
     gene            complement(11259..12008)
                     /locus_tag="PKN53_07010"
     CDS             complement(11259..12008)
                     /locus_tag="PKN53_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88734.1"
                     /translation="MKTKIFLVFITVFSVFTINAQISDSSINMLKFSINYSAHLPGGD
                     MAQRFGMSSMIGGSLEYKIASNWGFGLEFAYIFGGKVKDTTIFNNIATETGYIINQYG
                     EYATFTLSERGMYAGANLSKLIPVFGPNKNSGILVSLGAGLLQHHIHIENKDNNAPPI
                     LGDYKKGYDRLSNGLAVKEFIGYQHLDNKRLINFYIGFEFYQAFTMSRRDYDFNLMRY
                     DDTKRKDFLSGIRFGWIIPIYTKSPEKYYFY"
     gene            12305..15142
                     /locus_tag="PKN53_07015"
     CDS             12305..15142
                     /locus_tag="PKN53_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOD88735.1"
                     /translation="MRVFTLCIFSIFFSCITLFGQQQTELQKARTILNDRAEVFIKFS
                     ATPQELQHFSRILSIDNYDGENIFAYANAKEFENFLKTNKNFELVKDYYNRSRALTMA
                     TSIEEMQNWDKYPTYEVYVEMMQKFAQDYPNICKLDTIGFSTEGRSLLAVKISDNVNI
                     DEAEPEFLYTGQMHGDELIGGAVLLRLINYLLENYGSISGITDLINSVQIYINPLANP
                     DGTYRSDNSTVSNSIRYNAQSIDLNRNYKDFDAGNHPDGNEYGIETVAFMEYAENHKF
                     VMSANTHSGAELVNYPFDTHSELPADNLWWEFVSTEYATNAQENSASGYFTDQNNGVT
                     NGYAWYTITGSRQDYMNYYHNCREVTLELSTKKLLDAEDLPAHWEYNKKALLDYLKQV
                     TYGLRGIVTDSITHEPLQAKVWIENFDKLNSHVFSFPQHGDYYRLLKQGNYLVSFSAE
                     GYKTKTFEVEINDYQQTLLDVQLVNLENTSPLANFTSNQQIFECSPNVQFINLSEASS
                     DSEFLWDFGDGKTSTEFEPEHFYSQNGTYTVKLIAQNSFGENFIIKENFIEINLNEIP
                     ELPSFVICENSGNLEINLDLDGNILWFENINDTIPFNEGNSYTTPELHENTTYFIETI
                     FVGGNFNGGKLNRESGGSYVSDASNNYLIFDCYQNCILHSVKVYADGNGNRTIYLKNS
                     MGEILESKEIFIENGEQTVVLDFNLIQGEDFRLGCEATANLYRDFSGMFSNFNYPFNI
                     SNAISIKQSNTVWWYDEKKYYPYFYDWNISLPDCYSAKTPLNIYVNEEIVADFNFTIN
                     NNIVSFTNASIAADSYIWDFGDNTTSTEINPTHTYQSNGEYTVTLTASSGCGEDTISY
                     QVVIVAGIENHFTDNIEIYPNPSSDLLNIKSNQTISKIQILSVDGKIIKTFENINSTK
                     TFIDIENLNNSYYFINIYIENKIIRKPFIKL"
     gene            15155..>17171
                     /locus_tag="PKN53_07020"
     CDS             15155..>17171
                     /locus_tag="PKN53_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOD88736.1"
                     /translation="MKKFIFTLFAFLSCLWIFAQVSSPELDYAHNKLNERGEFYFKFK
                     CNDIELVNELSSKLSIDNFKNGFVYAYANQTEFETFLTYNLSFEPVYDYYETPKALTM
                     ATTVAQMANWDRYPTHAVFSQMMQNFVTNYPNLCVLENFGTSINGYPLVTLIISNNVN
                     TPSDKPEFWWSSTMHGDELTGYVLLLRFADYLLSNYGTDERVTNMLNNIKIHITPLAN
                     PDGTFYNSSNGTSVSYSRRANANNVDLNRNFPRVDGGTNNPIQNETQLTMDYANNHNI
                     VMAANIHGGTECMNYPWDSWTSSQNPHADQNWYYYISTVYVNEVRAVSPSSYFDGPES
                     YNTTGITHGGDWYVITGSRQDYFNYFKHIKELTVEISDNKQLDVEYLNAYWNYNKNAF
                     LSFTEQVLYGFRGIVTDACTGNALDGVKVEIAGHDTYNSFVYSFAPIGNYHRPIYEGD
                     YEVTFSKTGYVSKTLPVSVLNNQSTRLDVQLVPVNVAVPDFSTANTTIGIGSAISFQD
                     LTSGGTVTSRNWYFEGATPSTSSALNPTNITYNSTGNFDVKLEIVSNGCTIEELKSDY
                     IKVIIPGAPTALFTAQETSSCTGEIQFTNQSEGVGNSYLWNFGDGTSSTEENPLHVYT
                     QNGNFTVSLLVTNSVSDDSYTLPETITINRPEAPQITNANRCGTGSVN"
BASE COUNT         5510 a         2809 c         2420 g         6332 t
ORIGIN      
        1 ttttaaataa atacaaacaa gtttataatt attttcttta caccaagtcg ttgctattgt
       61 tttatatttt ttaagttgac tggagtgctc acctgtaaat attttatctt caataattat
      121 taaatattta tcattgatct caacccaaat atctatattt tcctgttgtc tcagggcaac
      181 aaccttagtt atattgatag ttttagtaaa ctcttttgaa agaagaaact ttataaagtt
      241 ttgagaacaa ttaaacaatt ttttattata ttttttatac tcaggatcac cccattgcaa
      301 taaccaagta aaaaagccat cttgtgacaa ctcttttgtt gcaatattaa ataaatttgg
      361 tttttttatc tttttcatat tcgcttgttt ctgattttac tgattacgtt gattatgctc
      421 aattataaga tcattaaaga tatcaaaaaa tatttttaac ttttcatttg gtggattgcc
      481 atcaaacagc cctaattctt gacaactaaa ttctgaatct ttaataaaaa ttgccttttt
      541 tttagaagtt gagtttttat agatttgcaa ctttactact tcattattat tccaaacacc
      601 tcttacatta cttgcaatat ctttccattt taaataacat ccccattccc tatagccggt
      661 cgcattaaca ttaaaacaaa tttttccatt ttcataatct tcccactttt taatttgttc
      721 ttgaatttta tgtcctttgt ctccatagcc actttttttg aaatttttga catttttgat
      781 atatgtatca tatcctctgc cgtttttgtc ctgagttaca caatactcta agtgagaaac
      841 aaaatactct aataatgatt taaaattttt atagtctttc atattaaaac acattttatt
      901 ttatacaaag atacatttta ttttctaaaa cacaaaattt tgacaacttt tttactttaa
      961 ttttaaatta tttcataaag tggattttgc ttatctgact tcaacatttt ttcaatcaaa
     1021 tttttgcatg ttgctgattt ataggtagat acgatttttt tcgggacacc tgcttgggtg
     1081 tcccaaattt taagttgtta gtattcaggg cgttagtcaa attagtttaa ttttaatttc
     1141 gtaattcctg ctttctgcga ttttatgttt gcttttcttt ataaactttg aactgtgtta
     1201 ttgtgttgcc caaattcagg taaaagttaa aaggtaaaag ttaaaagcgg ctcgcgggtt
     1261 cggctttctt tatgaacttt aaaaacttta taaactttta actgatttga ttgtgttgcc
     1321 cttgcgggca aaggcttttg agggtctaag caccgcaggc tgccgcctac ggttaatcac
     1381 atgttgtcct acggacaaac ctttgatagt ctgatacttg ctacaatacc aatgatgctc
     1441 gctcaaacac acatgatgct aagtggttgc aattacactc ggattacaac cgaagtccgc
     1501 aggacgagct cagcgaagct aaatccgagc gagcggagtt agttgttcta cgaacaaata
     1561 aatacgtaat cactgttttt attgttgtaa aaccaataaa caataaatca cacttgatta
     1621 tcaaaaagtg ttggttcttt aattggtttt tcaggttcgt aatttgttat taaaacttct
     1681 atgctcgcat ttttatcacg cacaatagtt tgataattag aatttgaata gttgtaatca
     1741 ataaaattaa ttttataatt tggattgtta ttaatccaag ttttcaaaat cttatttgat
     1801 ttccccttgt gttctaacac attagaaaga gcaaatttta tatttttccc attcaaatca
     1861 tctaacaaat tcaataattc ttcttcttct ttttctgtcc atcctttaaa accacgtttt
     1921 ccgtcattat aagttcctgt tgtaattaaa taaggtggat cgcaataaac aaaatcgttt
     1981 attccaaaat tggaaaaatc aaaatcatta aaacaaaaat cagaaaattc aatatttatc
     2041 tcttttattc taaggataaa acgcactaaa ttttgcctca ttgtagcatt aaaactactt
     2101 cgttcacgac caaaaggatt gttaaactcg tggctattat taaagcgaat ttgatgattg
     2161 aaagaaaatg caactaaaac aaacaaatct aaagggtttt tatgttcatt atactctttt
     2221 cgtaaatttt tataacctgt ttcatttgtc aaagacaaat taaaatcatt aattcgcttt
     2281 tcgatatatt tcaaagtatt atccatacta ttttgttgaa acactttgta catttcaatt
     2341 aaatagctca aaatatcgtt gcaataaatt ttattagcag ttacattaat tccaacatta
     2401 caacctcctg caaataaatc aataaaatta tcgatatgct ttggaaacaa aggcagaagt
     2461 tgaggcaaca gttttgattt tccgccaata taatttaatg gggattttat ttgtcttaat
     2521 ttgtatgttt gtaaaactga cattcttatt ttctattccc gcaatttaaa aataattttc
     2581 aaaaaatcaa tacaaatata agtattttaa ttcacaaaat gccccctaat actcaatatt
     2641 cttcctgtca acatagtctt tccaattttt gatttttgag tcagaagtga ttttttcttt
     2701 ttggtagtaa tggcacattg cagctgctaa accgtctgta gcgtcaagtt cttttgggag
     2761 ttcttcaagt tttaaaagtt tttgcaacat tcctgcaact tgctcttttg aagctgctcc
     2821 actacctgta attgctaact taatttttcg cggtgcatat tcataaattg gtattgttcg
     2881 agtaagtgcc gctgaaattg ccgctccttg tgctctgcca agttttagca tagactgcac
     2941 attctttccc tgaaaaggcg attctattgc aagctcatcg ggcttaaaac catctataag
     3001 ttgaattgtc ctttcgtaaa tatgctttaa tttattgaaa tgcccatcga gttttcgcat
     3061 gtcaacaact ccaaaaccaa cacattcaat agtttttcct ttttcatgaa taactccgta
     3121 acccataatg ttggttcctg ggtcaattcc taaaattact ctatcttttt ctaccttttc
     3181 catttatttg ctctcgctca aacgccgtaa attttttctt gcctctacag taaaaatact
     3241 gccaggatag tcttttatta attttaaata agcctcttga gctgtatcga aatgtctaag
     3301 tttttcttga caaactgcat atttaaaaag tgcattatcg cccaaaatat catagtaata
     3361 tgttgtagca acttgattgt aataagatga tgctttttcg taattgccaa tagcttgata
     3421 aatttctcct tttctataca atacatcatc aataagtcca tgatttggat aaagctcaac
     3481 aatagagtcc aagcctgcaa aagctctttc gtattgcttt gagaaaatgt aaaaatcggc
     3541 tctggcaaaa atttgcattg tggtttctgt tgtgtccaaa ttataattgt cggaaatgaa
     3601 catgctcaat tccatagcat cattagcaat aagtttagaa gtgctggctt tcaacacatc
     3661 taactgtgct tgtgcccatt taaactgtcc tgtataatat gcaagttttg cctttctaaa
     3721 tcttgcttca tgccctattg gacgctcagt ggcagctttt tcaacttctg cataagttaa
     3781 tatagcttcc caagtatttt cacttaaaaa ataaatatct gcaagcaaaa gttttaatgt
     3841 taatgtttgc tctgtaataa atctattatc ttctatgctt tttacagcca aattgattgc
     3901 actatcataa ttattcaaat aaaaagcatc taaatttatt attgcataaa ttattttata
     3961 agtttcttta cgtgggagga ctttcaagga ttcttcgagc ttgtttctta attgagttag
     4021 ctcgtctttg cttggctttt cttgatttaa agttttaaca taaagcacat tcaaactttc
     4081 gacataagca gaactataat atgcgttgtc tgtgccttta ttcataatat aattaaaagc
     4141 ctcgagagct aaatcgtatt ctaaattttc aaataaaatt attccataat ttaaaatatc
     4201 gctttcagat tttttatttc ttttgtctat tgcaaaaagt tgattgagag caagtctaaa
     4261 tcgccctgtt tgagtgtatt gccaaattat taattgtgaa tatacctcaa cttgcggttt
     4321 tttctgtatt ctgtccatta cgcttgtctc aacaatatcg tcaatgcttt ggtcaatatc
     4381 gtttgtcata acatattgta agcgcgattg aattacattc aaattttgag gatattcttc
     4441 caaataatca agataataac tcatcatttt aggataatct ctctgcacat aaaataaatt
     4501 tcccaactcc atagtgtaat cgtaatttgt gtttttcact ccttctaaat acactttttc
     4561 tgcatattca tattgacgat aacccgtgaa aacagcggcg gctgcattta tattatttct
     4621 atttaacttt acaatttcaa caactttatc aaattcggca attgcatctt taaaacggtt
     4681 ggtttgctga taaaccattc ctaaatctat cattaaataa aaatcatttg catttttttt
     4741 aatttctttt ttaagaaatt tttctgctgt atcaaattct ttaagttctg aaagacatct
     4801 taggtaatag tctctattgt ttttgtagcc gttttgccta tataattttt catataaagt
     4861 agctgctttt tcaaattctt tgttttgata atatctaaaa gctaactgag catcagcatt
     4921 ttgcgaaaag gcagaaaact ggctaaaaac tgtaaaaaca attagaaaaa taaaatattt
     4981 atatagttta aaattcactt ttaaatttta ttttgtaata atatcaaaac ctgtaaaatg
     5041 tcttaacact tctggcacaa ctattccttt ttctgtttga ttattttcta ataatgcagc
     5101 cataattcgt ggcaatgcca atgccgaacc atttaaacta tgtgccaagc gtgttttttt
     5161 ctcgttttct ggtctatatc ttagtttaag tctattggct tgaaaatctt cgaaattaga
     5221 aaccgaactt acttctaacc atctttcttg agcagcagaa aacacttcaa aatcaaaagt
     5281 caaagccgaa gtaaaactaa tatctccacc acaaagtttt aaaatacgat atggtaaatt
     5341 tagttttatc aataaagatt ctatatgatt tttcatttct tctaaaacag cataagaatt
     5401 ttctggctta gctatttgca caatttctac tttatcgaat tgatgtaagc gatttaatcc
     5461 tcttacatga gctccccaag aacctgcctc gcgtctgaaa caagctgaat aagcaacatt
     5521 ttttatagga aaatcttttt cttctaatat cacatttcta taaatgttgg taacaggcac
     5581 ttcagctgtt ggaattaagt aaaaattatc caaagtaata tgatacattt gtccttcttt
     5641 gtcgggaagt tgacctgtgc caaaaccaga atcttcgttc actaatagcg gtggttgaat
     5701 ttcggtatat cctgcttttt cggcttggtc taagaagaaa tttatcaaag ctctttgaag
     5761 ttttgctcct ttgcctttat aaacaggaaa tccagctcca gttatttttg taccaagctc
     5821 aaaatcaata atatcatatt cttttattaa atcccaatga ggttttttaa cagttaagtc
     5881 tggcattttt ccgccttctt taactatttc attatcttct gctgtttttc ctgcaggaac
     5941 agactcgtga gggatatttg gcaacaaaac gataatagaa tccatttctt tttcgacaga
     6001 gctgagcaag tcttcgaatt tttgcaattc ttctttaatg cctacagtct ttccctttgc
     6061 ttgttctgct tcgtcttttt tcttttgagc gtataaaatt ccaatttctt tagaaatatc
     6121 atttaattga gattgaagat tatctttatt aaattgataa tcttttcttt tattgtcgag
     6181 ttcaattatt ttttcaatta attcttccgc ctcaaagttt ttcactctaa gttgtttgat
     6241 aactctgtct ttttcttctc taattaatct taatgtcaac atagatataa attttctgcg
     6301 aagttagaaa aaatatcagt taaaagttaa aagttaaaag ttaaaagttg gatttttaaa
     6361 aaattaattt aaaaaccttt aataatgact gcgagttcaa cttttctttc ttcataaact
     6421 tttgctttca gcgagctcgt aaactcggtt gaaacttgag aaacttttaa ttatgtttat
     6481 tgtgttgccc ttgcgggcaa agcctttgag agggcatcat accgtaggct tcacctacgg
     6541 ttaatcacat gttgtcctac ggacaaacca cacacgctta cccgctcgct cggattttta
     6601 atccaagtgt aacattacca agcactcgga ttataaatcc gagcgagctt catgggtgtt
     6661 gaaagcgagc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggtgttgggg
     6781 gcgagcggat taggctgttt ataataagtt ttaaaacaac attcaaggtg tttcagaagt
     6841 gttaaaatgc aaggctctga aattgaggac gaatcagtta aaagttaaaa gtgggatttt
     6901 taaaaaaatt tataaaaaaa cagagaacct atttaaaaat tctctgtttt tgctttatac
     6961 ttacttaaaa acttaaactt ctttcatctt ttgactttta acacttcaac tacgttcagt
     7021 gcatcgcttt caacttttaa ctacatttag agtgtccaca ttctgtgcaa attacgcagc
     7081 cgtcttggta aacttgagta tcttcttgtt gacagccttc gcaaatatct ccttttttag
     7141 aagctttggt tccattaggg atatattttc gtaaagctct ttcaacaccg cgtttccaag
     7201 tgttaatgct gtcgttatca aattctaatg aagatactaa ttccacagct ttatctatag
     7261 ccattccgtg tcgtaatact cctgagatta gttttgcata gttccagaaa gttttatcaa
     7321 attgatgcga caatccttgg aaagttgtag catagccata tctgtttgtg tattggaagt
     7381 cgtatctatt atttccttct gcatctcttt ctttaattat tctaccattt tctattgctt
     7441 taggaatatt taaagtatct tcttcgactc tacctgtaaa gatttcgtat ggcattccgt
     7501 ttaataatcc aacaaaagct atccattctt cgtcgtcgtt tctaaatcta actatatctg
     7561 catctagaac ttttggacgt ttaagttcaa gcatttgttt tgcttgttga ttatttttat
     7621 cgtcagaaat taacacgcct gctctagaac cttctctata aactgtaatt cctttacatc
     7681 cacatttcca agcttcgaaa taacattctt caactatttc ttccgaaaca ttttctggca
     7741 tatttatagt tacgctaatg ctatggtcaa cccatttttg aattgctcct tgcattctaa
     7801 ctttgcttaa ataatcaata tctgctgcag ttgctttgta atatggtgat ttttttacaa
     7861 gttcttcgac ttggtcatta gacatatttt taacttcttc aacatcatat ttattaattt
     7921 gaagccatgt taaaaattta tggtggaaaa ctttgtattc ttcccatttg tcgccttgtt
     7981 cgtcaacgaa atcgactctt acttctgggt cgtttggatt aacttttctt cgtctagtgt
     8041 aaaatggtct aaacacaggt tctattcccg atgttgtttg tgtcattagg cttgtagttc
     8101 ctgtaggagc aattgttaaa agagaaatat ttcttcttcc ataattttcc atttctctta
     8161 ccatttctgg gtctgcttgt cttagccttt cgataaatgg attatttttt tctctttggc
     8221 tatcgtaaat cataaatggc attctttctt tagccatatt tacggaagat ctatatgctt
     8281 ctattgcaag agttttgtga acttctgttg caaattcgtt tgctgtatca gttccataag
     8341 ttagtcctaa tgctgccaac atatcgcctt cggcagtaat gcctactcca gttcttcttc
     8401 cttcttcggc tttgcgtcta atattttccc aaagttttct ttcaactctt tttacttcta
     8461 tatcttcggg gtcggaattg atttttgcta gaatttggtc tattttttct aattctaaat
     8521 caataatatc gtccatcatt ttttgagcat agccaacgtg ttttttaaac ttgtcgaaat
     8581 taaacttagc ttcttttgta aatgggttat ccacataaga atataaattt attgctagca
     8641 atctacaaga gtcgtatggg caaagcggaa tttctccaca aggatttgta gaaacagttt
     8701 tatatcccaa atctgcataa caatctgcta ccgactcgcg tattatagtg tcccagaaca
     8761 aaattcctgg ttcagcagat ttccatgcat tatgaataat ttttttccaa attttttgtg
     8821 ggtcaacttc cttaatatac ttaggtgctc tatcatctat tgggaattgt tgaataaact
     8881 tatctttatt aataagtgca ttcataaact catcagtaag ttttacagaa acatttgctc
     8941 ctgtaacttt accttgagtt aattttacgt caataaaatc ttcgctatca gggtgtttta
     9001 ccgaaaccga aagcatcaac gctcctcttc ttccgtcttg agcaacttcg cgagtagtgt
     9061 tagaatatct ttccataaaa ggcacaattc cagtagaagt tagggctgaa ttttttactg
     9121 gcgagccttt tggtcttaaa tgtgaaaggt cgtgtccaac gcctccacgt cttttcatca
     9181 gctgaacttg ttcttggtca atttttaaaa ttccaccgta agaatctgtt ggtttgtcgt
     9241 gtccaattac aaaacaattt gataaagaaa caatttgcag attgttacca atacccgaca
     9301 ttggaccacc ttgaggaact atatatttaa aatctttcaa taattcataa atagtttctt
     9361 ccgaaagagg attatcgtat ttcgtttcta ttcgatatat ctctttagcc aaacgtctgt
     9421 gcatatcgtc tggagtaagt tcgtacaaat tgccatctga atcttttagt gcgtatttat
     9481 taatccacac tcttgcagca aggtcatcgc ccttaaaata ctcttgacta gctaaaaaag
     9541 cttcagaatc gctaaaaatc tttctcttta cttcttcctt aaaattctgt gtcatttgtt
     9601 tattttccaa gttcattttt tattatactt tttaatatct gatttttaaa aaaacaaatc
     9661 ccaaaatatt gggaaaattt gcaagtacta aaatatagag tttagacaaa aaaacaaaaa
     9721 acagtttatc aacaattgtt gggaaataat ttaaaaactt cttaaaaagt cataaatcaa
     9781 gcacttaaat atttgcaaga aattttgaga aaaaagaaat taaccgttca aaaaccttaa
     9841 caaataagca attcaacaat tttaaattta ttttttaaca actcattgtt caattatcca
     9901 atccctttat tctttttgtg gcatttgttt ataaatcaaa ctattacatt tttcaaagaa
     9961 atatttaaaa aaaaagatat tcacatttta aaagtaaaaa aatgctcttt aaaacatcat
    10021 tttttttcat agataatgta aattatcaaa tattttatgc gtagaaccaa aagaattgtt
    10081 tacgtacgaa tttgcattaa ttttacactt ttctatcaca tttacatcac taatcaacac
    10141 attcattata tttttatatt cttcagagtt gtttatgcta aaagcaactt cgttttttat
    10201 taattgtaca gcttcgttaa actttttgtg atttggtcca aaaactactg gaatttcata
    10261 aacaacagct tctaaaatat tatgaattcc ttttccaaaa cctcctccaa tatatgcaat
    10321 atttgcataa cgatataatt ttgacaaaat tccaatagaa tcaattataa ttaatcgtgt
    10381 gtcgggattt agggtttcta tcttagataa tctttgactt gtaattttaa gtcttttttc
    10441 taagtttttt atattctgtt cagaaatttc gtgaggagca ataataaaaa cataatcttc
    10501 aggcgaagag tttatgaagt ttgttataat tttttcgtct gctgcccaag tgcttccata
    10561 aacaaaacat tttttgcctt cgcaaaattt ttcaagaatt ggattttcat atttttcatt
    10621 agctatagca acaactctat caattcttgt atctccgcaa actaaagtgt ttttgaaatt
    10681 gtatttatgt aataattttt ctgtttcttc tgtttgcaca aatattttgc taaaaaaacc
    10741 taaggctttg cgataagttt ttccataaga cttaaaaaac acttgatttg gacgaaaaat
    10801 agcagcaatt aaataagttg ggattttgtt tttatttaat ttttctaaga aataatacca
    10861 tatttcatat tttacaaaaa ttgcagtaac tgggtttact gtttcaataa attcttttgc
    10921 attttttcgt gaatcgaaag gcaaatagca aactatatct gcaaattggt aattttttcg
    10981 aggttcgtaa ccacttggac taaaaaaact taaaataatt ttatattctg gtttttcagt
    11041 ttttattttt tcaattagtg gtctgccttg ttcaaattca cctaaagacg cacagtgaaa
    11101 ccatatatat tttgagtttt tatcaatttt agatttaatt tcttgaattt gattttttct
    11161 gccttcaatc attaattttg cttttttatt aaagattgaa gcaattttta ttgcaaaaaa
    11221 atatgattga gtaattattt tataagcaaa aagcatattt aataaaagta atatttttct
    11281 ggtgattttg tataaattgg aattatccat ccaaatctaa taccacttaa aaaatctttt
    11341 cgttttgtat catcatatct cataagatta aagtcataat ctcttcggct cattgtaaaa
    11401 gcttgataaa actcaaatcc tatataaaaa ttaattagtc gtttattatc caaatgttga
    11461 taacctataa attcttttac tgcaagtcca ttgcttagtc tgtcgtatcc ctttttataa
    11521 tcacctaaaa ttggcggtgc gttattgtct ttattttcga tatgaatatg atgctgcaaa
    11581 agtcctgctc caaggcttac taaaatccca gaatttttat ttggtccaaa aacaggaata
    11641 agtttactta gattagctcc tgcgtacata cctctttctg acaaagtgaa ggttgcatat
    11701 tcaccatatt gattaataat gtatcctgtt tctgttgcaa tattattaaa aatagtagtg
    11761 tcttttactt ttccgccgaa gatataagca aattctaacc caaatcccca attagaagct
    11821 attttatatt ctaaacttcc accaatcata gaagacattc caaaacgttg agccatatcg
    11881 ccaccaggaa gatgagcaga ataattaatg gaaaatttta acatatttat actagagtcg
    11941 ctaatttgag cgtttatagt aaaaacagaa aaaaccgtaa taaaaaccaa aaaaatcttt
    12001 gttttcattt tgcaaaatta taaaaactta acatatctta aaacaaaaaa acattgtgaa
    12061 tattgtaaaa tatttagatt ttttgacact gttgctcaaa atgccaaact gatatgccaa
    12121 tcctgataaa aataaatatt atttttctta ttatacacaa aaataaaaac tttcattttt
    12181 tacataattt gccgtatatt taaacggttc tattaaaata aatggcgatt atatttttta
    12241 tcaatttttc gtctaacata aactttttct tcgtaacttt gaaatctaaa taattctttt
    12301 tctgatgaga gtttttactt tgtgcatatt ttctatattt tttagttgta ttaccttatt
    12361 tggacagcaa caaactgaac ttcagaaagc aaggactatt ttaaacgaca gagctgaagt
    12421 ttttattaaa ttttctgcta ctccacagga attacaacat ttttcaagaa ttttatctat
    12481 tgataattat gatggagaaa atatttttgc ttacgccaat gccaaagagt ttgaaaattt
    12541 cttaaaaact aataaaaatt ttgaacttgt taaagattat tataatcgct caagagcttt
    12601 gactatggca acaagcattg aagaaatgca aaattgggat aaatatccaa cttatgaggt
    12661 ttatgttgaa atgatgcaaa aattcgcaca agattatcct aatatttgca agttagatac
    12721 cattggtttt tctactgaag gacgttcgct tttagcggtt aaaatttctg acaacgtaaa
    12781 tattgacgaa gctgaaccag aatttttata tacaggtcaa atgcatggag atgagcttat
    12841 tggtggagct gttttattga gattgataaa ctatttatta gaaaattatg gttctatttc
    12901 aggaattacc gatttaatta attctgttca aatttatata aatccgcttg caaatcctga
    12961 tggcacttat aggtcagaca atagcacagt gagcaattcg attagatata atgctcaaag
    13021 cattgattta aacagaaatt acaaagactt tgacgctggc aaccatcctg atggcaatga
    13081 gtatggtata gaaactgtag cttttatgga atatgctgaa aatcacaagt ttgtaatgtc
    13141 agcaaatact catagcggag cagaacttgt aaattatcct ttcgatacgc attcagaatt
    13201 gcctgcagat aatttatggt gggaatttgt ttctacagaa tatgcaacta atgctcaaga
    13261 aaactcggct tcgggttatt ttacagacca aaacaacgga gttacaaatg gttacgcttg
    13321 gtacactatt actggaagtc gtcaagatta tatgaattac tatcataatt gtcgagaagt
    13381 aactttggaa ttaagtacta aaaaactttt agatgcagaa gacttacctg ctcattggga
    13441 atacaataaa aaagctttgt tagattattt aaaacaagtt acttatggtt tgcgaggcat
    13501 agttacagac tctataactc acgaaccatt gcaagcaaaa gtgtggatag aaaattttga
    13561 caaattaaat tctcatgtgt tttctttccc tcaacacggc gattattacc gtttgttaaa
    13621 acaaggaaat tatcttgtta gtttttctgc agaaggctat aaaacaaaaa cttttgaagt
    13681 cgaaattaat gattaccaac aaactctgct tgatgttcag cttgttaatt tggaaaacac
    13741 atctcctttg gcaaatttta cttcaaatca gcaaattttt gaatgttcgc caaatgtaca
    13801 gtttataaat ttatccgaag cctcatctga ttccgaattt ttgtgggatt ttggcgatgg
    13861 aaaaactagt acagaatttg agccagaaca tttttattct caaaacggaa cttatacagt
    13921 aaaacttatt gctcagaact cttttggaga aaattttatt ataaaagaaa attttattga
    13981 aattaattta aatgaaattc ctgagcttcc aagttttgtg atttgcgaaa attctggcaa
    14041 tcttgaaatt aatcttgatt tagatggaaa tattttatgg tttgaaaata taaacgatac
    14101 aattccattt aatgaaggaa attcttacac aacacctgaa ctacacgaaa acacaacata
    14161 ttttattgaa acaatatttg tcggtggcaa ctttaatggt ggaaaattaa atcgcgaaag
    14221 cggaggaagt tatgtgtctg acgcatctaa taattattta attttcgatt gttatcaaaa
    14281 ttgcattttg cactctgtaa aagtttatgc cgatggaaat gggaatagaa caatttattt
    14341 gaaaaattca atgggcgaaa ttcttgaatc aaaagaaata tttattgaaa acggagaaca
    14401 aactgttgtt ttagatttta atttaataca aggcgaagat tttagattag gatgcgaagc
    14461 gactgctaat ttatacagag attttagtgg aatgttttca aattttaatt atccattcaa
    14521 tattagcaat gcaatttcta ttaaacaaag caatactgtt tggtggtatg atgagaaaaa
    14581 atattatcca tatttttatg attggaatat aagcttgcct gattgttatt ctgcaaaaac
    14641 tccattaaat atttatgtaa acgaagaaat tgttgctgat tttaatttta ctattaataa
    14701 taatatcgtt tcttttacaa acgcttcaat tgcagcagat tcttatattt gggattttgg
    14761 tgacaacaca acaagcacag aaataaatcc aactcacact tatcaaagca atggagaata
    14821 tacagttaca ttaacagctt caagcggatg tggtgaagac actatttctt atcaagttgt
    14881 tattgttgct ggaatagaaa atcattttac agataatata gaaatttatc ctaacccaag
    14941 ttcagattta ttaaacatta aatcaaatca aacgatttct aaaatccaaa ttttaagtgt
    15001 tgatggtaaa attattaaaa cttttgaaaa tattaattca actaaaactt ttatagacat
    15061 tgaaaatttg aataattcat attatttcat taatatttac attgaaaata aaattatcag
    15121 aaaacctttt ataaaacttt aaaatctact tattatgaaa aaatttattt ttacattatt
    15181 cgctttttta tcctgcttat ggattttcgc acaagtttca agcccagagc ttgactatgc
    15241 tcataacaaa ctaaatgaac gcggagaatt ttattttaaa tttaagtgca atgacataga
    15301 acttgttaat gaattatctt cgaaattatc aatagataat tttaaaaacg gttttgttta
    15361 tgcttatgca aatcaaacag aatttgaaac ttttttaact tacaatttaa gttttgagcc
    15421 tgtttatgat tattacgaaa cacccaaagc cttaacaatg gctaccacag ttgcacaaat
    15481 ggcaaattgg gacaggtatc caactcatgc ggttttttcg caaatgatgc aaaatttcgt
    15541 aactaactat cctaatttat gtgttttaga aaattttgga acatcaatta atggttatcc
    15601 tttagtaact ttaataattt caaataatgt aaacacacca agtgacaaac ctgagttttg
    15661 gtggtcgagt accatgcacg gtgatgaatt aactggctat gttttattgc ttcgttttgc
    15721 agattatctt ttatcaaatt acggtacaga cgagagagtt accaatatgt taaacaatat
    15781 aaaaattcac ataactcctt tagcaaatcc tgatggaact ttttataatt caagcaatgg
    15841 aacttcggtg tcttattcga gaagagctaa cgctaataat gttgacttaa atagaaattt
    15901 tcctcgtgta gatggaggaa ctaataatcc tattcaaaac gaaacacaat taacaatgga
    15961 ttatgccaat aatcacaata ttgttatggc agcaaatatt cacggtggta ctgaatgcat
    16021 gaattaccct tgggactctt ggacaagctc ccaaaatcca catgcagacc aaaactggta
    16081 ttattatata agtactgttt atgttaacga ggttagagct gtttcgcctt caagttattt
    16141 tgatggaccc gaatcatata atactactgg aattactcat ggtggagatt ggtatgttat
    16201 tactggttct cgtcaagatt attttaatta ttttaaacat attaaagaac ttactgtaga
    16261 aatttctgat aacaaacaac ttgatgttga atatttaaat gcctattgga attacaataa
    16321 aaatgctttt ctttcattta cagaacaagt actttatggt tttagaggaa ttgttaccga
    16381 tgcttgtact ggaaatgcat tagatggtgt taaagttgaa attgctgggc atgacactta
    16441 taattcattt gtttattctt ttgctcctat tggaaattat catcgtccaa tttacgaagg
    16501 agattatgaa gtaacttttt ctaaaacagg atatgtaagt aaaactttgc cggtaagtgt
    16561 tctaaacaat caatcaacaa gattagatgt gcaattagtt ccagtcaatg tagcagttcc
    16621 tgatttttca actgctaata caactattgg aattggttca gcaattagtt ttcaagattt
    16681 aacatctggt ggtacagtta cttccagaaa ttggtatttt gaaggtgcta caccaagcac
    16741 aagttctgca ctcaatccta caaatattac ttataattct actggaaatt ttgatgtaaa
    16801 attagaaatt gtaagcaatg gctgtactat tgaagaactc aaatcagatt atataaaagt
    16861 aattattcct ggtgcaccaa cagctttatt tactgcacaa gaaacttctt cttgcactgg
    16921 cgaaatacaa tttacaaatc aaagtgaagg agttggtaac tcttacttgt ggaattttgg
    16981 tgatggcaca agttctacag aagaaaatcc attacatgtt tatacccaaa atggcaattt
    17041 tactgttagt ttattagtta caaactctgt tagcgacgac agctatacat tacctgaaac
    17101 aattacaata aatcgtcctg aagcacctca aataaccaac gcaaacagat gcggaacagg
    17161 ttctgtaaac t
//
LOCUS       DAOPNX010000053        17138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_3982_length_17138_cov_6.340572, whole genome shotgun sequence.
ACCESSION   DAOPNX010000053 DAOPNX010000000
VERSION     DAOPNX010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17138
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3982_length_17138_cov_6.340572"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PKN53_07025"
     CDS             complement(<1..353)
                     /locus_tag="PKN53_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOD88737.1"
                     /translation="MLNDKELLKIFNDYLLIQDFGKTPANTYDPIYYTLKSGGKRIRP
                     VLCLMATELFGETYKTSLDIACGLEIFHNFTLLHDDMMDNSDVRRGNPTVHIKWTDNI
                     ALLSGDAMSVIAYKY"
     gene            complement(366..1334)
                     /locus_tag="PKN53_07030"
     CDS             complement(366..1334)
                     /locus_tag="PKN53_07030"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOD88738.1"
                     /translation="MKTLTPIHKFKRKILVTGGAGFIASSLAEKLAEDKENLVVVLDN
                     LSTGNIDKLSHDSDNLRFIKADVNKYVEIMEVMLSYQFHYVFHYAAMVGVLRTQKNPI
                     GVLDDIKGIENICRLSKNIGVRRVFYASSSEVYGEPVEFPQNAHTTPLNSRIPYAIVK
                     NLGEAFLKSYQKEFGLNYTIFRFFNTYGPKQSKDFVISKFLYAALNNHDITIYGDGSQ
                     TRTFCYIDDNIEACTKIAYNDLFINEVVNIGNSIETSINELAEIIIRLTKSKSKIINV
                     PPLAEGDMKRRCPDNSDMLKVLNRKLISLEDGIGKILKTGLFEITH"
     gene            complement(1349..3775)
                     /locus_tag="PKN53_07035"
     CDS             complement(1349..3775)
                     /locus_tag="PKN53_07035"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOD88739.1"
                     /translation="MWNKVARVILRYRIALLIIVGLITIFMGYQASLIEMDYHYASML
                     SKKHPVLIDNVKFKQTFGEEGNGIFVGFESDDFFELKNFQALQNLCEELKKIDRVTNV
                     LTVSSAVNARQITVTDENGVNKRQFEIYNIFPDKVTSQAELDSLKEVFFSLPFYENLL
                     YNKEKNVFMMNVSIDKEILNSKARIPVVLKVEEVVKEFAEAQNIEVYVSGLPFIRTKM
                     MDLIKSEIILFIVLAALVCVIILYLYFRSFKVIGVAMLVVGVSVTWAVGFMGIFGFRI
                     TILSGMVPPLLIVIGIPNTIYLLNRYHTETKAHGNKILALQRVISKIGNAVFLSNATT
                     AVGFTSFIITNSSVMVEFGIIATLGIISTFINALIMLPAIFSFLQPPSSKYTKHLNNK
                     YLSKVLNGITNIVTNHRRKIYATIAVILILSAIGVTLMKRTGYILDDVSQDSEIYVAT
                     KFLEKNFGGVSPVEIAVQSKDSLKGYEMITQMEKIDSLQQKLKQYPELSRSLSIVDAL
                     KFLNQAYSKGDADKYALPSSPKTYEIIFQRLPEGGLNQNIAQSFIDSTRTITRISLNI
                     EDIGTNRMKELIPKIENDINTVFPKDKYNTLITGNSILYFTGTSYLINNLISSLALAI
                     VLIGLLMLGLQRSFKMVGILLIPNLIPMLVTAGLMGYFGVPIKPSTILIFGISLGISV
                     DGAIHFITRYRQEMFVNGWNVVGAVKKAISETGISMVYTYSILFFGFLIFTFSEFGGI
                     TALGFLISMTLLVAMIGNLILLPSMLISFEKYITKKDFPEPIMEIYEDDDEDNEEVEI
                     NEIEEKDKIY"
     gene            complement(3866..5152)
                     /locus_tag="PKN53_07040"
     CDS             complement(3866..5152)
                     /locus_tag="PKN53_07040"
                     /inference="COORDINATES: protein motif:HMM:NF017396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S28 family serine protease"
                     /protein_id="HOD88740.1"
                     /translation="MMKFLRFVFVSVFLTLSYSLIASELENLLKGLDGVVSVEELPTT
                     EFFSEKYLIIFEQKVDKDDDDSEVFYQRVFLNHKSFTAPVIFTTEGYSADYAMNKNYI
                     NELCSILNGNEICVEHRYFSKSKPENIDWHYLTTRNAAEDHHIIRKTLGQLYKGKWVS
                     TGISKGGQTCLLYQMYYPNDVDACVAYVAPIAKALEDGRHEPFINNVATKKERKKVSD
                     FQLEILKRRETIFPMFEQFCKASKLSFQLPLEEIYDYCVLEYSFAFWQWVGNTERIPS
                     KKEDDSKLFQHWVAVAGPDYFSIESSENVLPFYYQAARELGYYGYETTLFRKYLKIKT
                     AKNYFKKVFLTKDMTPEFYSQTSIDLEKFVQTKAKNTILVYGEYDPWTAVAPEIVNTA
                     DLVKVVKPKGTHSTRIKNLPKVQHDMIINLLNSFME"
     gene            5271..8651
                     /gene="mfd"
                     /locus_tag="PKN53_07045"
     CDS             5271..8651
                     /gene="mfd"
                     /locus_tag="PKN53_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOD88741.1"
                     /translation="MKNLLNRLFDSNSNIEELLSILDNNSNAKSIILKGLSGSSKAFI
                     LAKLSQENKNLTCVILPDKESASYFYDDLNNIIGDKNILFYPSAYKRSIEYLKTDKTN
                     IVLKTEVLNKISSNKKPYLIITYPGAIIEKVISNKTLTKNTLEIKQGEKLSMDFVIEV
                     LNEYGFTATDFVYEPGSFSVRGSLIDIFSFSDEFPFRVDFFGDEIESIRSFDPQTQLS
                     TEKHKKIVIIPNVHEHFDETEKVSFFGFINKNCKFWISEPELVLDKIEDTYTAAEQRY
                     KEVEDSDFDDEDNVILEPSKNLLNVQEFTNQIKNFQIVEIASRSVFEAEKEFYFNTEP
                     QPKFGKQFNILAEILQNNEANGYETYVLSNNEKQLIRLKDIFTEQNQIVNFIPVLKIV
                     NQGFIMHESKTCVFTDHQIFERYHRFSSKKLSHKDSITIQELTALRPGDYVVHIDNGV
                     GKFGGLQKINNNGKIQEVVSIIYDNNDILYVNIHSLHRISKYRDKDDEPPKIYRLGSG
                     HWKRLKEKTKKNIKDIAKDLILLYAKRREQKGFAFSPDSYLQHQLESSFMYEDTPDQS
                     KANELIKADMESEVPMDRLVCGDVGFGKTELAIRAAFKAVNDSKQVAVLVPTTILALQ
                     HYHTFKERLADMPVNIAYVSRLKSSKEIKQSLQKLEEGKLDIIIGTHRLLNKDVIFKD
                     LGLLIIDEEQKFGVSMKEKLKNLKINVDTLTLTATPIPRTLQFSLMGARDLSVINTPP
                     PNRQPIITELHSFNNKIIQDAIYYEVGRNGQVFFINNRIQNIFEIETLVNKLCPEVKT
                     VVAHGQMESQKLEKIILEFMRGDYDVLISTTIIENGVDIPNANTIIINNANNFGLSDL
                     HQLRGRVGRSNRKAFCYLLSPPLSVLTPEARRRLKAIEEYSELGSGMNIALQDLDIRG
                     AGNILGAEQSGFIADVGFETYNKILNEALRELKEGKFDDTFSDNEQNEEDEDLISVDY
                     DCQIETDMDILIPDYYVSSTSERIKLYKELDEIKDENSLSIFKINLEDRFGKIPKETE
                     RIFELIKLKWLAIKIGFQKIVIRNNTLLAYFPSNQQSNYYSSPKFAKVLNYVQHNPQN
                     LQIKEINEKLSLKVLKVHKLEDIAKVLNGILLAVENNS"
     gene            complement(8664..9683)
                     /locus_tag="PKN53_07050"
     CDS             complement(8664..9683)
                     /locus_tag="PKN53_07050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOD88742.1"
                     /translation="MKNKNIFIQLPLLVIGLFLVFAFGCKEEEKIPTVSTTEITNITM
                     SSATSGGFIEDDGGAGIIDCGVCWSTNDNPTIADNKTIDTLGSFGFESSITGLEANTT
                     YYVRAYATNSVGTGYGNVISFKTSGFSTFTDPRDGNVYKTIQIGNQIWMAENLKYLPS
                     VVSPEVGSDTYPYYYVYGYSGNNISDAKATENYATYGVLYNWSAVMGGAMSSDNNPSG
                     VRGVCPEGWHVPSEAEWTELTDFLGGEIVAGGKLKETGTEHWKTPNEGATNEVGFTAL
                     PNGARAHFGAYFFSLGENGFWWTTTKGTDADDAKCVFMFYDYGNINVQASSKTLGGAV
                     RCVKN"
     gene            complement(9720..10634)
                     /gene="miaA"
                     /locus_tag="PKN53_07055"
     CDS             complement(9720..10634)
                     /gene="miaA"
                     /locus_tag="PKN53_07055"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOD88743.1"
                     /translation="MLKDYNLIVITGATAGSKTSVAAHLAYDLKTEIISADSRQVYKT
                     MNIGTGKDLEDYEVNGVKIPYHLIDICEPGYKYNVYEYKRDFFKVYSSLRERGIVPIL
                     CGGTGLYINAVINNYELVKVPVNEKLRAELKSKSIQELEVILRKYKDLHNNSDLDTTK
                     RAIRAIEIAEYYSRNSFVGDNYPEIKPIIFGIYFEREQRRARITERLKFRLENGMIEE
                     AQAMLDAGISHQDLEYYGLEYKFLSWYLKGDITYDELFTQLNTAIHQFSKRQMTWFRK
                     MEKEGSEIIWIDGNLDMESKLKLIKSYL"
     gene            complement(10729..11676)
                     /locus_tag="PKN53_07060"
     CDS             complement(10729..11676)
                     /locus_tag="PKN53_07060"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HOD88744.1"
                     /translation="MSNKCLYCYKDLNTEGDFHTSCSLSFFGSPKPPALNYTIDEMAE
                     LARQVVENSITVPGVQPKLSLSFIKDVLKDETNGRLTVLGAMGGNYILKPQNKVYPQM
                     PENEHLSMRMAELCGISVVPSSLIRLKSGELSYITKRIDRTQTGQKIHMLDMFQILEA
                     FDKYKSSVEKIGRALAKHSANTVLDLLRLFEIVVFSYLTGNNDMHLKNFSMILKNEEW
                     NLSPAYDLLNVNLHLPEDNEEFALTINGKKRKLTKSDFVNLGLKLQLTEKQISNTFDR
                     FIKAENIMKQEISNSFLSTENQMKYIDLLEVRLSLFKEK"
     gene            complement(11669..12004)
                     /locus_tag="PKN53_07065"
     CDS             complement(11669..12004)
                     /locus_tag="PKN53_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HOD88745.1"
                     /translation="MRQGKVFYNDDFAGILTETDDGEYLFAYDKEYIKQFPNQAISFS
                     MPVSDKVYRDNRLFPFFEGLIPEGWLLEIAYESWKLNRNDRMGLLLACCKNCIGAVSV
                     IPLINEKDE"
     gene            complement(12001..12213)
                     /locus_tag="PKN53_07070"
     CDS             complement(12001..12213)
                     /locus_tag="PKN53_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOD88746.1"
                     /translation="MVLSLATFVKTKRKEAELTQQEFADKAGVALTVVRKIEQGKENL
                     SMAKVNQVLMMFGHKLAPVKHKEIEL"
     gene            12505..>13033
                     /locus_tag="PKN53_07075"
     CDS             12505..>13033
                     /locus_tag="PKN53_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOD88747.1"
                     /translation="MSDRYKIFDQNAVYFTTLTIVNWIEVFTSKEQKHMIVDALKYCQ
                     KEKGLEIYGWCLMPSHLHLICSVSSEIGMSGFLRDFKKFTSKEIVKLINETTENRKEW
                     ILEQFKRACKHLKRDQKYKVWQDSNQTKIIFSTNFFYQKLKYIHDNPVEDMLVTNPED
                     YMFSSARNYADLDYLL"
     assembly_gap    13034..13133
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13397..13972
                     /locus_tag="PKN53_07080"
     CDS             13397..13972
                     /locus_tag="PKN53_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88748.1"
                     /translation="MKSQDLIKRIVRILLNPIAGWDIHKPEDGEFSFKTIKKPIFVVT
                     LIILFLARLVGKTISELATTDFIYILGFSVSMLIVDFLYFFLLIFILNYLTPFYNIKA
                     GKAKIAFLIYTCLLPFYFCIIVYNLFPALYFLNVIIVYSLFLFYWGLKNYLKVQSKDA
                     LIIFITFSLIMLGSYLILNFLFVRPFFDFTF"
     gene            13976..14428
                     /gene="smpB"
                     /locus_tag="PKN53_07085"
     CDS             13976..14428
                     /gene="smpB"
                     /locus_tag="PKN53_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOD88749.1"
                     /translation="MSTKVSIYKNKKAYYQYEFIEKYVAGIVLLGTEIKSIRDGKINF
                     ADSYCVFRNGELWATGIHIAEYVLGTCNNHDPRRDRKLLLNRRELNKLQRKVSEKGLT
                     IIPVCMILNEKGIAKLEIALARGKKLHDKREDLKTKDSKRDIERYSKF"
     gene            14431..15060
                     /gene="plsY"
                     /locus_tag="PKN53_07090"
     CDS             14431..15060
                     /gene="plsY"
                     /locus_tag="PKN53_07090"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOD88750.1"
                     /translation="MLTYIIIFSILSYLIGSFSSAVWFGGWFWNTDVREFGSKNAGAT
                     NTFRVLGAKAGLIVFVFDVLKSFAAIQLVRLVPELQQNSELYIQIKILFGACAIIGHI
                     FPVFSQFRGGKGVASMLGFIIAIHPLAAGITFGVFLIVFLISRIVSLSSISAALAFPF
                     IIYFLEKGNSLTLTIFSIIATCLIILTHLKNIKRLLKGEEKPISFSKKS"
     gene            15086..17137
                     /locus_tag="PKN53_07095"
     CDS             15086..17137
                     /locus_tag="PKN53_07095"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HOD88751.1"
                     /translation="MKTIYYYLATFITLVSVISCNSGNKEMKTEKQTPKAIDLADLDT
                     TISPTEDFYQYATGGWQKNNPLPDEESRFGSFDLLAKETNQKVNDIIVNLTKQENAEN
                     SIEWKIATFYNLGMDSVKLDKDGIKPIEEYLKKIDAISSKDDVLKTIAEFHRLGISSS
                     FAIFGSADRENSKMEIAYLYQGGLGLPNRDYYTADDARSIEIREEYKKHIDKMFDLFK
                     INNTKSEAISKIIFDFEYKLADNSMTNLELRDPYATTNRMSQSELNEISPDFNFETYF
                     SYIKLPQQEVVNVSQPKFFKEFNNVFKNTDIETWKLYLKWNLIRTSSQYLSTEIADAS
                     WEFYGKFLTGALVQQPRWRKVVNRTNSSMGEAVGQIFVREYFPPEAKQRMEVLVENLR
                     AAFAKRIEKLDWMSDVTKVQAKEKLAAINVKIGYPNKWRDYSSLKIKNDYYYANILRA
                     NEFDFDDMLSKIGKPVDKEQWFMNPQTVNAYYSASMNEICFPAGILQAPFFYLDADDA
                     VNYGAIGVVIGHEMTHGFDDKGRSYDKDGNLKDWWTEEDAKRFNEKSQVLVERFNNID
                     VLPDLKADGKLSLGENIADYGGLKISYDALTIALGNETPEKIDGFTAKQRFFLSYAKI
                     WGQNIRDTEIRRRTKEDVHSLGQWRVNGQLPGIKEFHDAFNVKPGDKMYLPPEKWATI
                     W"
BASE COUNT         5980 a         2699 c         2448 g         5911 t
ORIGIN      
        1 atatatttgt aagcaattac cgacattgca tctcccgaca acaaagctat attgtctgtc
       61 catttaatat gtactgttgg atttcctctt ctgacatcag aattatccat catatcatcg
      121 tgtaaaagag taaaattatg gaatatttct agaccacaag caatatctaa agatgtttta
      181 tatgtttcgc caaataattc tgttgccata aggcacaaaa caggacgtat tcttttacct
      241 ccagatttta aagtgtagta aattgggtcg taagtattgg ctggagtttt tccaaaatct
      301 tgtatcaata aataatcatt gaatattttt agtagttctt tatcgtttag catttttaaa
      361 ataatttagt gtgtaatttc aaatagtcct gtttttaaaa tttttccaat tccgtcttct
      421 aaacttatta gttttctatt caaaacttta agcatatccg aattatctgg gcatcttctt
      481 ttcatatctc cctcggcaag aggtggcaca ttaattattt ttgattttga ttttgttagt
      541 cgtattatta tttctgcaag ttcattaata gaagtttcaa tagaatttcc aatattaaca
      601 acttcgttta taaataaatc gttataagca atttttgtgc aagcttcaat attatcgtct
      661 atatagcaaa aagttcttgt ttgtgagcca tctccataaa ttgtaatatc atgattattt
      721 agagcagcat aaagaaattt tgaaattaca aaatctttac tttgcttagg accataagtg
      781 ttgaaaaatc taaaaattgt ataatttaaa ccaaattctt tttgatatga ttttaagaaa
      841 gcttcgccaa gatttttcac tattgcatat ggaattcttg agtttagtgg tgttgtatgt
      901 gcgttttgag gaaattcaac aggctcgcca taaacttctg atgatgatgc ataaaatact
      961 cgtctaactc ctatattttt acttagtcgg caaatatttt caattccttt aatatcgtct
     1021 aaaacaccta taggattttt ttgagttctc aacactccaa ccatagctgc ataatgaaaa
     1081 acataatgaa attgatatga aagcattact tccataattt ctacatattt atttacatca
     1141 gctttaataa atcttaaatt atctgaatca tgagacaatt tgtcgatatt accagttgac
     1201 aaattatcta gcacaacaac tagattttct ttatcttcag caagtttttc agctaaagaa
     1261 cttgcaataa aacctgctcc tcctgtaact aaaattttgc gtttaaactt gtgtattgga
     1321 gttaatgttt tcatgttttt ttattttttt aataaatttt atctttttct tcaatttcat
     1381 ttatttcaac ttcttcgttg tcttcatcgt catcttcata tatttccata attggttctg
     1441 ggaagtcttt tttagtaata tatttttcaa aacttatcag catagatggt agtaatatta
     1501 aatttcctat catagcaact agtaaagtca ttgatattaa aaatcctaaa gcagttattc
     1561 caccaaattc agaaaatgta aaaattaaga agccaaaaaa cagaattgaa taagtgtaaa
     1621 ccatgcttat tcctgtctcg ctaattgctt ttttaactgc acctacaaca ttccaaccat
     1681 ttacaaacat ttcttgtcta tatcttgtaa taaagtgtat tgcaccgtca acagatattc
     1741 ctaaagatat tccaaaaatt aaaattgtac ttggtttaat aggaacacca aaatatccca
     1801 ttaatccagc agttactagc attgggatta agtttggaat cagtaaaatt cctaccattt
     1861 taaacgaacg ttgtaagcca agcattaata aacctatcaa aacaatagct aatgctaagc
     1921 tactaattaa attgtttatc aaataactag tacctgtaaa atataaaatt gaatttccag
     1981 taatcaaagt attgtattta tctttaggaa agacagtatt tatatcgttt tcaatttttg
     2041 gtataagttc tttcattcta ttagttccaa tatcttcaat atttaaacta attctagtaa
     2101 tagttctagt gctatcaata aaagattgag caatattttg atttaatcct ccttcgggta
     2161 atctttggaa gataatctca taggtttttg gtgatgaagg caatgcgtat ttatctgcat
     2221 ctcctttaga atatgcctga tttagaaact ttaaggcatc aactatagac agagaacgag
     2281 ataactctgg atattgtttt agtttttgtt gtaaagagtc tatcttttcc atctgtgtta
     2341 tcatttcata tccttttaag ctgtctttag attgtactgc tatttcaaca gggctaacac
     2401 ctccaaaatt cttttctaaa aatttagtgg ctacataaat ctcagaatct tgtgaaacgt
     2461 catctaaaat atatcctgtt cttttcatta gagtaacgcc aatagcagat aatattagta
     2521 ttacagcaat agtagcataa atttttcttc tatgatttgt tacaatattt gtaattccat
     2581 ttaaaacttt agataaatat ttattattaa gatgttttgt atatttactt gaaggtggtt
     2641 gcaaaaagct aaaaattgct ggaagcatta ttaatgcgtt gataaaagtt gatattattc
     2701 ctaaagtagc aattattcca aattctacca ttacagagct atttgtaatt ataaagcttg
     2761 taaatccaac tgcagtagtt gcattactta aaaatactgc atttccaatt tttgaaataa
     2821 ctctttgcag agctaaaatt ttatttccat gagcttttgt ctctgtatga tatctattta
     2881 ataaataaat tgtatttgga attccaataa ctattaacag aggcggaacc attcccgata
     2941 agattgtaat tctaaatcca aatatcccca taaacccaac tgcccaagtt acgctcactc
     3001 ctactacgag catggctacg cctataactt taaatgatcg gaaatataaa tataaaatta
     3061 taacacaaac taaagcagct aaaacaataa acaatattat ttcgcttttt attaaatcca
     3121 tcattttggt tctaataaaa ggtaaacccg aaacatatac ttcgatattt tgtgcctctg
     3181 caaactcttt tactacttct tctactttta atacaactgg aattcgagct ttgctgttta
     3241 gaatttcttt gtcaatagac acattcatca taaacacgtt tttctctttg ttgtatagta
     3301 agttttcata aaaaggtaaa gagaaaaaca cttcttttaa gctatctaat tctgcttgac
     3361 ttgtaacttt atctggaaaa atattgtaaa tttcaaactg tcttttgttt acaccatttt
     3421 catctgtaac tgtaatttgt ctagcattta cagcagaact aacagttaaa acattggtaa
     3481 cgcgatctat tttttttaat tcttcacata agttttgtaa tgcttgaaaa tttttcagtt
     3541 caaaaaaatc atctgattca aaacctacaa atataccatt tccttcttca ccaaaagttt
     3601 gtttaaactt tacattatct attaaaactg gatgtttttt cgaaagcatt gaggcgtaat
     3661 gatagtccat ctctataaga gaggcttgat accccatgaa aattgttatt aaaccaacaa
     3721 ttattaaaag tgctattcta taccttaaaa ttacccgtgc tactttattc cacattatta
     3781 ttaatcaatt gtttaagaat ttgcaaaatt acaatattga ttttagtaaa caagaaaaat
     3841 ttgaaataat tcagttttta tacaattatt ccataaaact attaagcaaa ttaataatca
     3901 tatcatgttg tacttttggt agatttttaa ttctggtaga gtgagttccc tttggtttta
     3961 caactttaac aaggtcggca gtatttacaa tttctggagc aaccgctgtc catggatcgt
     4021 attcgccata aactaatata gtatttttag cttttgtttg aacaaatttt tccaaatcaa
     4081 tagatgtttg agagtaaaat tcaggagtca tatctttagt taaaaatacc tttttaaaat
     4141 aattttttgc ggttttaatt tttaaatatt tcctaaataa tgtagtttcg taaccataat
     4201 accctaattc tcttgccgct tgataataaa atggcaaaac attttctgaa ctttctatag
     4261 aaaaatagtc tggtccagcc actgctaccc agtgttgaaa gagcttagaa tcatcttctt
     4321 ttttgcttgg aattctttcg gtattaccaa cccattgcca aaaagcaaat gaatattcta
     4381 aaacacaata atcgtaaatt tcttctaatg gaagttgaaa actaagtttt gaagctttac
     4441 aaaattgttc aaacatagga aaaatagttt cacggcgttt taaaatttct aattgaaaat
     4501 cactaacttt ttttctttct ttttttgttg caacattatt tataaaaggt tcatgtctgc
     4561 cgtcttctaa ggctttagct attggtgcca cataagctac acaagcgtca acgtcatttg
     4621 gataatacat ttgatataaa agacaagttt gcccaccttt gctaattcct gtactaaccc
     4681 atttgccttt atataattgt cccaaagttt ttcttataat atgatggtct tctgcggcat
     4741 ttcgtgttgt taaataatgc caatctatat tttcaggctt tgattttgaa aaataacgat
     4801 gctcaacaca aatttcgttt ccattcaaga tagaacataa ttcatttata tagttcttat
     4861 tcatcgcata atcagcagaa tagccttctg ttgtaaaaat aacaggtgca gtaaaacttt
     4921 tgtgatttaa aaacactcgt tgataaaaaa cttctgaatc atcatcatct ttgtcaactt
     4981 tttgctcaaa aattattaaa tacttctcag aaaaaaattc tgttgtagga agttcttcta
     5041 cagatacaac tccatctaaa cctttcaata aattttctaa ctcactagca attaagctat
     5101 aagaaagagt taaaaatacg cttacaaata caaatcttaa aaacttcatc attctataat
     5161 tttaaaaaat atttgtcgca gtatataaaa tttaatagaa atcaatttgt aaaatatcaa
     5221 gattttatca acaaatttat gtttacttat ttttttaata attttgcgga gtgaaaaatt
     5281 tattaaatag actttttgac tcaaattcta atatagaaga attgctatct atattagaca
     5341 acaatagtaa tgctaagtcc ataatactga aaggcttatc aggctcatct aaagctttta
     5401 ttttagcaaa attaagtcag gaaaataaga atttaacttg cgtaatacta cccgacaaag
     5461 aaagtgcttc ttacttttat gatgatttaa acaatattat aggcgacaaa aatattttgt
     5521 tttatccttc ggcttacaag cgctcaattg aatatttaaa aacagacaaa accaatattg
     5581 ttttaaaaac agaagtttta aacaaaatat cttccaacaa aaagccttat ctcatcatta
     5641 cttatcctgg tgcaataatt gaaaaagtta tttcaaacaa aactctaaca aagaacacat
     5701 tggaaataaa gcaaggcgaa aagctttcta tggactttgt tattgaagtt ttaaatgaat
     5761 atggatttac tgcaactgat tttgtttatg aacccggaag cttctctgtt agaggaagtt
     5821 taatagatat tttttctttt tctgatgaat ttccatttag agttgatttc tttggcgatg
     5881 aaattgaaag tattagatct tttgacccac aaactcagct ttcaaccgaa aaacacaaaa
     5941 aaatagtaat aattccaaac gttcacgaac actttgatga aacagagaaa gtttcgtttt
     6001 tcggttttat aaacaaaaat tgtaagtttt ggatttcaga accagaatta gttttagata
     6061 aaattgaaga tacatatact gctgctgaac aaagatacaa agaagttgaa gacagtgatt
     6121 ttgatgatga agataatgtt attttagaac caagcaaaaa cttattaaat gttcaagaat
     6181 ttacaaatca gataaaaaac tttcaaattg ttgaaatagc ttctcgcagt gtttttgaag
     6241 cagaaaaaga gttttacttc aatactgaac cacaacctaa atttggaaag caatttaata
     6301 ttttagcaga aattcttcaa aacaacgaag caaatggtta cgaaacttat gttttaagca
     6361 ataacgaaaa gcaattaata agacttaaag atatttttac tgaacaaaat caaatagtaa
     6421 actttattcc agttttaaag attgtaaacc aaggttttat aatgcatgaa tcaaaaacat
     6481 gtgtttttac cgaccaccaa atttttgaaa gataccacag gtttagcagt aaaaaacttt
     6541 cgcacaaaga tagcattaca attcaagaac ttacagcttt acgccctggc gattatgttg
     6601 tacatattga taatggagtt ggaaaatttg gcggtttaca aaaaataaac aacaacggca
     6661 aaatccaaga agtggtaagc attatctatg acaacaacga tattctttat gtaaatattc
     6721 actctttaca cagaatttca aaatatagag ataaagatga tgaaccgcca aaaatttatc
     6781 gcttgggttc tggacattgg aaacgattga aagaaaaaac caagaaaaac ataaaagata
     6841 ttgctaaaga tttgatttta ctttatgcaa aacgcagaga gcagaaaggt ttcgcatttt
     6901 cgcctgactc ctatttacag catcaattag agtcatcttt tatgtacgaa gacactcctg
     6961 accaaagcaa agccaatgaa cttataaaag ccgatatgga atccgaagtt ccaatggaca
     7021 gattagtttg tggtgatgtg ggttttggaa agacagaact tgcaattaga gcagctttta
     7081 aagcagttaa cgacagcaaa caagtagcgg tattagttcc tacaacaatt ttagcacttc
     7141 agcactacca tacttttaaa gaaagacttg cagatatgcc tgtaaacata gcctatgtaa
     7201 gcagattaaa atcaagcaaa gagataaaac aaagtttaca aaaattagaa gaaggcaaac
     7261 ttgacataat tataggcact cacagattgt taaataaaga tgtgattttc aaagacttag
     7321 gcttgttaat aattgacgaa gaacaaaagt ttggtgtgtc tatgaaagaa aaattgaaaa
     7381 atcttaaaat aaatgttgac actctaactc ttacagcaac gccaatacca cgaactttgc
     7441 aattttcttt aatgggtgcc agagatttat ctgtaatcaa cacgccacca ccaaacagac
     7501 aaccgataat tactgagctt cattcattta acaacaaaat tattcaagat gctatttatt
     7561 acgaagttgg aagaaatggt caagttttct ttataaataa tagaattcaa aatatttttg
     7621 aaatagaaac tttggtcaac aaactttgtc ctgaagtaaa aactgttgta gcacatggac
     7681 aaatggaaag tcagaagtta gagaaaatta ttttggaatt tatgcgtggc gattatgatg
     7741 tgttaattag tacaacaatt attgaaaatg gagttgatat tccaaatgca aatacaataa
     7801 ttattaacaa tgctaacaac tttggtttga gcgacttaca ccagctcaga ggaagggttg
     7861 gtagaagtaa tagaaaggct ttttgttatt tgctttctcc cccattgtct gtgcttactc
     7921 ccgaagctcg caggcgttta aaagcgatag aagaatacag cgaattaggc tcaggaatga
     7981 atatagcttt acaagattta gatattcgtg gtgctggcaa tattcttgga gcagaacaat
     8041 ctggatttat tgctgatgtt ggctttgaaa cttataacaa aatacttaac gaagctctcc
     8101 gagaattaaa agaaggaaaa tttgacgaca cattttcaga taatgaacaa aacgaagaag
     8161 atgaagattt aatttcagta gattacgatt gtcaaattga aacagatatg gacatcttaa
     8221 tccctgacta ttacgtaagt tcaacatctg aaagaattaa attatacaaa gagttagacg
     8281 aaattaaaga tgaaaattca ttaagtattt ttaaaataaa tttagaagat agatttggca
     8341 aaattcctaa agaaacagaa agaatttttg aattaattaa acttaaatgg ctcgcaataa
     8401 aaataggatt tcaaaaaatt gttattagaa acaatacttt attagcttat tttccaagca
     8461 atcagcagtc taactattat tcttcgccaa aatttgccaa agttttaaac tatgttcaac
     8521 ataatcctca aaatttacaa attaaagaaa ttaacgaaaa actaagttta aaagttttga
     8581 aagttcacaa attagaagat attgctaagg ttttgaatgg aatactatta gcagttgaaa
     8641 ataattctta attctaaatt actttaattt ttcacacaac gtacagcccc acctagagtt
     8701 ttagaacttg cttgaacatt aatatttcca taatcataaa acatgaaaac acattttgca
     8761 tcatcggcat ctgttccttt tgtagtggtc caccaaaaac cgttttctcc aaggctaaag
     8821 aaatatgcac caaaatgagc gcgagcgcca ttaggaagag ctgtgaaacc aacttcattt
     8881 gttgctcctt cgttaggtgt tttccaatgt tctgttccag tttctttaag tttgccacct
     8941 gcaacaattt ctccacctaa aaagtctgtt aattctgtcc attctgcttc gcttggcaca
     9001 tgccaacctt ctggacaaac accacgcact ccactaggat tattatcgct actcattgct
     9061 ccacccataa ctgccgacca attatataaa acgccgtaag tggcataatt ttctgttgct
     9121 ttagcatctg aaatattgtt tccactatag ccataaacgt aataatatgg atatgtatca
     9181 gaaccaactt caggactaac tacgcttggc aaatatttta aattttctgc catccaaatt
     9241 tggttaccaa tttgtatagt tttataaaca ttgccatcgc gaggatctgt gaaagtgcta
     9301 aatccgctag ttttgaaaga aataacatta ccataacctg tgcctacact atttgttgca
     9361 taagctctca catagtaagt tgtgtttgct tccaaacctg taatactact ttcaaagcca
     9421 aatgaaccaa gagtatcaat agttttatta tctgcaatag ttggattatc gtttgtactc
     9481 caacaaactc cacaatctat tatccctgca ccaccgtcat cttcaataaa acctcctgat
     9541 gtggctgaag acatagtaat atttgttatt tctgtagttg acacagtagg aattttttcc
     9601 tcttctttgc aaccaaatgc aaacactaaa aataatccaa ttactaataa tggcaattga
     9661 ataaagatgt ttttgttttt cattttataa aaatttaatt gttaatacat ttttaaattt
     9721 cacaaataag atttaataag tttcaatttg ctttccatat ccaaattccc gtctatccaa
     9781 attatttcgc ttccttcttt ttccattttt ctaaaccaag tcatttgtcg tttcgaaaat
     9841 tgatgaatag cagtatttaa ttgagtaaat agctcatcat aagtaatatc gcccttcaaa
     9901 taccaagaca aaaacttata ttctaatccg taatattcca aatcttgatg agaaattcca
     9961 gcatctaaca tagcttgggc ttcttctatc attccattct ctaagcgaaa tttaagtctt
    10021 tctgtaattc ttgctctcct ttgctcacgt tcgaaataaa ttccaaaaat tattggttta
    10081 atttctgggt aattatcgcc tacaaaacta tttcttgaat aatattctgc tatttctatt
    10141 gctcgtatag ctcgttttgt ggtatcgagg tcgctattat tatgtaaatc tttgtatttt
    10201 cgtaaaatca cttcaagttc ttgaatagat ttgcttttta attctgctcg taatttttcg
    10261 tttacaggaa cttttactaa ttcataatta tttataactg catttatata taaacctgtt
    10321 cctccacata atattgggac tattcccctt tccctcaaac ttgaataaac cttaaagaaa
    10381 tctcttttat actcataaac attgtattta tagccaggtt cgcaaatatc tattaaatga
    10441 taaggaattt ttactccatt aacttcataa tcttccaaat ctttacctgt gccaatattc
    10501 attgttttat aaacttgcct actatcagca ctaataattt cggtttttaa atcatatgcc
    10561 aaatgtgctg ctacagaagt tttactcccc gcagttgcac ctgtaataac aattaaatta
    10621 tagtctttta gcaatttaaa atgatttaaa gtttaagaca aagatataat tttttagtta
    10681 gaaaatgttt gttacataaa ttttattatc aacaataaaa tttcaaatct acttctcctt
    10741 aaataaagac aatctaactt ctaacaaatc aatatatttc atctgatttt ctgtcgaaag
    10801 aaaagagtta cttatctcct gtttcattat gttttctgct ttgataaatc tatcaaaagt
    10861 atttgaaatc tgtttttcag ttaattgaag tttcaatcct aaatttacaa aatctgattt
    10921 cgtgagtttt cttttctttc cgtttatagt taatgcaaat tcttcattat cttcaggaag
    10981 gtgtaggttt acattcagca aatcgtatgc aggcgacaaa ttccattctt cattttttag
    11041 aatcattgaa aagtttttca agtgcatgtc attatttcct gtaaggtagc taaaaacaac
    11101 tatttcaaaa agtcttaaca aatccaaaac tgtatttgca gaatgttttg ctaaggctct
    11161 tcctatcttt tctacagagc ttttatattt atcaaaagct tctaaaatct gaaacatatc
    11221 taacatgtgt attttctgac ctgtttgagt gcggtcaata cgtttggtta tatatgaaag
    11281 ttcgccagat tttagcctaa ttagcgatga aggaactaca gaaatcccgc aaagttcagc
    11341 cattctcatg cttagatgct cattttcagg catttgagga taaactttat tttgaggttt
    11401 taaaatataa tttccaccca tagctcctaa cactgttaat cgtccattag tttcatcttt
    11461 taaaacatct ttaataaaac ttagagaaag ctttggctga actccaggaa cagttattga
    11521 attttctact acttgtcttg ctaattctgc catttcgtct atggtgtaat tcaaggcagg
    11581 cggtttggga ctaccaaaaa aactaagact acaagaagta tgaaaatctc cttctgtatt
    11641 caagtcctta tagcaatata aacatttgtt actcatcttt ttcgtttatt agtggaatta
    11701 cacttacagc accaatgcag tttttacaac atgctaaaag caagcccatg cggtcgtttc
    11761 gatttaattt ccaactttcg taagcgattt ctaaaagcca accttcaggt attaaacctt
    11821 caaaaaaagg aaataaacga ttgtcacgat acactttatc cgaaacaggc atagaaaaac
    11881 ttattgcttg attaggaaat tgtttgatgt attccttatc gtatgcaaat aaatattctc
    11941 catcatcagt ttctgtaaga atacctgcaa aatcatcatt ataaaatact tttccttgtc
    12001 tcataactca atttccttat gtttaacagg tgctaactta tgcccaaaca tcatcaatac
    12061 ctgatttact tttgccatac taagattttc tttgccttgt tcaatttttc taacaacggt
    12121 aagtgctaca ccagctttat cggcaaattc ttgctgtgta agctcggctt ctttacgctt
    12181 tgttttaaca aatgtggcta aacttaatac cataattata tgtttttgca gttattttgt
    12241 tgcaaagata taaaattata ttcaattgca tataattttg tgaaaattaa atgatttata
    12301 tgcaaaaaga tatactatgt cgctcgctcc caacacccat gtcgctcgct cggatttgca
    12361 atccgagtgt tatacaactc ttccgctcac tccttaccac aatgaagctc gctcggattt
    12421 gcaatccgag tgttactaaa tatttttcgt aatctaaatg ttgcaaataa ttaattattt
    12481 ctatattttt ataaaaaaat cttaatgagc gacagatata aaatattcga tcaaaatgct
    12541 gtttatttca cgacattaac gattgttaat tggatagaag tttttacaag taaagaacaa
    12601 aaacatatga ttgttgatgc attaaaatac tgtcaaaaag aaaaaggttt agaaatttat
    12661 ggttggtgtt taatgccaag ccatttacat ttaatttgta gtgtttcatc agaaatagga
    12721 atgtcggggt tcttgcgtga ttttaagaaa tttacatcaa aagaaatagt taaattgatt
    12781 aatgaaacga cagaaaatcg caaagaatgg attttagaac aattcaagag agcatgtaaa
    12841 cacttaaaac gtgatcaaaa atataaagtc tggcaagaca gtaatcaaac aaaaataatt
    12901 ttctcaacta acttttttta tcagaaacta aaatacattc atgataatcc tgtagaagat
    12961 atgttggtta ctaatccaga agattatatg tttagctctg ctcgaaatta tgctgattta
    13021 gattatttat tagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatgaaga
    13141 ttatatgttt agctctgctc gaaattatgc tgatttagat tatttattgg aagtaattgt
    13201 ggaaccacaa caattagtta cagtaaaata aaaaaatagc atatccaagc actcggattg
    13261 caaatccgag cgagcaatga agtagtaccc gcacttattg caaccaaacc ataacaaaca
    13321 agcataagaa aaataaacct catttcttct taaaaatact aaaaaataaa ttatttttgt
    13381 aaattcttta aaaacaatga aaagtcaaga tttaataaag agaatagtta ggattttgct
    13441 aaaccctatt gcaggctggg atattcacaa gcccgaagat ggagaattta gctttaaaac
    13501 tataaaaaaa cctatttttg tagttacact aataatttta tttttagcaa gacttgttgg
    13561 caaaacaatt tccgaattag caacaacaga ttttatttat attttaggct tttctgtgtc
    13621 aatgctgatt gttgacttcc tttatttctt tcttttaatt ttcatattaa actatcttac
    13681 tccattttac aatattaaag caggaaaagc taaaatcgcc tttttaattt acacatgttt
    13741 attgcctttt tatttttgca ttattgtcta taaccttttt cctgcccttt attttttaaa
    13801 cgtaataata gtttattcgt tatttttatt ttattgggga ctaaagaatt atttaaaagt
    13861 tcaaagtaaa gatgctttga ttatttttat aacattttcg ttgataatgt taggttctta
    13921 cttgatttta aactttcttt ttgttcgtcc gttttttgat tttacatttt aaattatgag
    13981 tacaaaagtt tctatatata aaaataaaaa agcctattat caatacgaat ttattgagaa
    14041 atatgttgct ggaattgtgc tgttaggaac ggaaataaaa tctattcgtg atggcaaaat
    14101 taactttgca gattcttact gtgtttttcg taatggcgaa ttatgggcaa caggaattca
    14161 tattgctgaa tatgttttag gaacttgcaa taaccacgac ccacgcagag atagaaaact
    14221 tttgctaaac agacgggaat taaataaatt acaaagaaaa gttagcgaaa aaggtcttac
    14281 tattattcct gtttgcatga ttttaaacga aaaaggaatt gctaaattag aaattgcatt
    14341 agcacgtgga aaaaaactac acgacaaacg cgaagattta aaaactaaag attcaaaacg
    14401 agatatagaa cgctacagta aattttaaac atgcttactt acattatcat attttcaatt
    14461 ttatcttact taataggctc atttagttct gctgtttggt ttggaggctg gttttggaat
    14521 acagacgtaa gagaatttgg aagtaaaaat gcaggtgcta caaatacttt tagagtttta
    14581 ggggcaaaag caggacttat tgtttttgtt tttgatgttt taaaaagttt cgctgcaatt
    14641 caacttgttc gccttgtgcc agaactacaa caaaatagcg aactttatat tcaaatcaag
    14701 attctttttg gtgcatgtgc tataattgga catatttttc cagtattttc acaatttaga
    14761 ggcggaaaag gtgtcgcatc tatgttagga tttattattg caattcatcc attagccgca
    14821 ggaattactt ttggagtgtt tttaatagta tttctaatta gcagaattgt ttctctttca
    14881 tcaatttcgg cagcattggc ttttcctttc ataatttatt ttctcgaaaa aggaaattca
    14941 ttgactttaa ctattttttc tataatagct acttgcttaa ttattttaac tcatttaaaa
    15001 aatattaaaa gattgttaaa aggcgaagag aagcctataa gtttctctaa aaaatcttag
    15061 ctttgcgaaa aaatgagtaa aaattatgaa aactatttat tattatttag cgacttttat
    15121 cactttagta agtgtaattt catgtaattc gggaaataaa gaaatgaaaa cagaaaaaca
    15181 aacaccaaaa gcaatcgacc tcgctgattt agataccaca atcagtccga cagaagattt
    15241 ttaccaatat gcaactggcg gttggcaaaa aaacaaccca ctaccagacg aagaatcaag
    15301 atttggttct ttcgacctat tggctaaaga aactaaccaa aaagttaatg atataattgt
    15361 taatttaaca aagcaagaaa atgcagaaaa tagcattgaa tggaaaattg caactttcta
    15421 caacttggga atggacagtg ttaaattaga taaagatgga attaagccta ttgaagaata
    15481 tttgaaaaaa attgatgcaa tttcaagcaa agacgatgtt ttaaaaacta ttgctgaatt
    15541 tcatagatta ggaatttcaa gctcttttgc aatttttggc tcggctgata gagaaaacag
    15601 taaaatggaa attgcttatt tatatcaagg tggtttgggt ttgccaaata gagattacta
    15661 cactgccgat gatgcaaggt ctattgaaat tagagaagaa tataaaaagc atatcgacaa
    15721 gatgtttgat ttattcaaaa taaacaatac taaatcagaa gctatatcaa aaataatatt
    15781 tgattttgaa tataaacttg ctgacaattc tatgactaac ttggaattaa gagaccctta
    15841 tgcaacaact aacagaatga gccaaagcga gttaaatgaa atttctccag attttaattt
    15901 tgaaacatat ttttcttata taaaacttcc acaacaagaa gtggttaatg ttagtcaacc
    15961 taagtttttc aaagagttta acaatgtttt caaaaacaca gatattgaaa cttggaaatt
    16021 atatttaaaa tggaatttaa ttagaacttc atctcaatat ttaagcacag aaattgctga
    16081 tgcttcttgg gaattttatg gtaaattttt aacaggtgct ttagttcaac aaccacgttg
    16141 gcgtaaagtt gtaaatagga ctaactcaag catgggcgaa gctgttggac aaatttttgt
    16201 aagagaatat tttccaccag aagcaaaaca aagaatggaa gttttggtag aaaatttaag
    16261 agctgctttt gctaaaagaa ttgaaaaact cgactggatg agcgatgtta caaaagtaca
    16321 agctaaagaa aaattagcag caataaatgt aaaaattggt tatccaaaca aatggcgtga
    16381 ttattcaagt ttaaaaatta aaaacgacta ttactatgct aacatcttgc gtgccaatga
    16441 gtttgacttt gatgacatgc tttcaaaaat tggaaaacct gttgataaag aacaatggtt
    16501 tatgaatcct caaactgtaa atgcttatta cagtgcaagc atgaacgaaa tatgtttccc
    16561 tgcaggaatt ttgcaagctc cattcttcta tttagatgct gatgatgctg ttaattatgg
    16621 tgctataggc gtagtcatag gacacgaaat gactcacggc tttgatgata aaggaagatc
    16681 ttatgacaaa gatggaaacc taaaagattg gtggacagaa gaagacgcta aacgttttaa
    16741 cgaaaaatct caagttttag ttgaacgctt taataatatt gatgttttac cagacttaaa
    16801 agctgacggc aaattatctt taggcgaaaa tattgcagac tacggcggat taaaaatctc
    16861 ttatgatgct cttacaattg ctttaggtaa tgaaactcct gaaaaaatag acggttttac
    16921 tgctaaacaa agatttttcc tttcttatgc aaaaatttgg ggacaaaata ttagagatac
    16981 agaaattcgc agaagaacaa aagaagatgt acactcactt ggtcaatggc gtgtaaacgg
    17041 acaattacca ggaattaaag aatttcatga tgctttcaac gtaaaaccag gcgacaaaat
    17101 gtatctacct cctgaaaaat gggcgactat atggtaaa
//
LOCUS       DAOPNX010000054        17067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_4003_length_17067_cov_7.161651, whole genome shotgun sequence.
ACCESSION   DAOPNX010000054 DAOPNX010000000
VERSION     DAOPNX010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17067
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4003_length_17067_cov_7.161651"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1729
                     /locus_tag="PKN53_07100"
     CDS             <1..1729
                     /locus_tag="PKN53_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOD88752.1"
                     /translation="NGYYYFTINNVHFFADTTKNEHEVDLKISLRKSFAEDDIYNSEG
                     FIYQTIRNINIYSDYDPKQAANNTEQYLASFNKQNINGINLFYKEKLQLKPSVILQSC
                     FLNLGDRYNIVNVEKTHSHLANLKQFKIINIKLNASEDVFLDSQKERFLDVNIYLTPL
                     KRQNYSLELEGNNTSGNIGMGGAITYNNKNIAKRAQMFSAKAMLSFQTLKAEENTERL
                     KFFNTLEYGFDLGVHIPRLLVPFWDNYEFSKNHIPKTHVTMSLNYQQRPDYTRYIAKS
                     AFGYVWKGGKNNYITHMVNPIELYSVKILDFNPEFQKQIENLYIKYSYQDQLLSVISY
                     NFIFNNQSLTKSRDFSYLWLNLETSGNLPYLAYKGLNIEPVDGSYKFLGLEFAQYAKI
                     DLDYRYYQVFDEKHSLVYRGFVGVGFPYGNSTSGLPFIKKYFVGGANDLRAWRVRSIG
                     PGTYSNPNRNYDQIGDMKFVFNLEYRFPIISLVNGAVFLDAGNIWAVDKNDNRAGALF
                     NLYRFYNEIAIGTGLGARLDLSFFVIRFDFGIPIHDPSLAQGDRWLSAFKDFKLRNFT
                     MNFGIGYPF"
     gene            1738..2268
                     /locus_tag="PKN53_07105"
     CDS             1738..2268
                     /locus_tag="PKN53_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88753.1"
                     /translation="MIKKTFFILVLVCSQFCLFSQNHVFLGINPSVNFNRDYPSGAFD
                     MNVLPLTIEIPIINNVDIRLISKLNYGFRTTGGALVGVGGEVGLPVYIHRGEFENNIP
                     TGFYISPGFLYLKNVFYKHSHKAIFIEPGYNFAFKNKFGLCLGLQYGRKFLNYTDGSK
                     LQANHFGLKIILGFWL"
     gene            2369..3259
                     /locus_tag="PKN53_07110"
     CDS             2369..3259
                     /locus_tag="PKN53_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88754.1"
                     /translation="MFIANPIYDTVFKYMMSDTKVAKIFLSAIIEEKIVELDFASTER
                     SLKPGAKRSDINRELEELVVCRFDFAAKIKTGSGEYKTVTIELQKAKFATDIMRFRRY
                     LGVMYQDADNTSDKQRLKARQIYCIYLLNYDIGLSNSPVIKVDHIVSDQATGEELSNK
                     NEFIQSLNHKSWIVQIRQLKENRRNDLENLLSIFDQDNITKDNHILNVNSENFSEEHK
                     HIVRKLQEAYASSQIRDEMIMEDDYFKELEVKDDIIEEQKKKLVKNAKTIAEKDKTIA
                     EKDKEIAELKRLSELKNKEK"
     gene            3339..3794
                     /locus_tag="PKN53_07115"
     CDS             3339..3794
                     /locus_tag="PKN53_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88755.1"
                     /translation="MKNIFILFTTLILFVSSNGFFVEQYLCNACNEEHSEITFFEFGE
                     YSHNHKHYTACDHSHSNCLCSQSDEDHLEHAKVSFYSFDKLFFEYELVKIPQIKFLDN
                     FNLQVFPIDINKELLSFNSFVETLLKIPSLFTYQSVGQDVCTLISVFRL"
     gene            3852..6059
                     /locus_tag="PKN53_07120"
     CDS             3852..6059
                     /locus_tag="PKN53_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOD88756.1"
                     /translation="MKKIYSIIFNVLFVLTLNAQVSGIVYDNSDKKPMFGVNIWWKNT
                     NIGTTSDIYGKFELSKPKESNKLIFSFVGYNTDTIEVLKNNQKLEVFLNSNIILDEVI
                     VGERRSGTHYSRMEVGNVQTISGAELCKAACCNLSESFETNASVDASYSDATTGAKQI
                     RLLGLAGKYVQMMTENIPNLYGLSQPYALGYIPGPWMESIQVSKGTSAVINGYDAITG
                     QINIEYKKPKTSDALSFNQLFSSSGRAETNIDASFILNPKWSTMIFAHSQHDFLEIDE
                     NKDGFIDMPKMRQNILFNRWDYLGKYLTIRFGAQYIDEVRKSGQTSKFLKNNEITNPY
                     KIFLGTKRFQGFYKMGYVFPKKDYKSLAMVTSFTYHDQNSIYGKTNFDATQQSAYLNI
                     IWQSAFAGNEEHKYNAGLSFKYENLDQLLKDSTMLNQEIVPGGYFQYTANFPFNLHLI
                     AGLRADYHNQEGLLITPRLNIKYNITGALSWRASVGKGYRYANVLAENNYLLASSRKI
                     IIAPDLKLEEAWNFGSNITWYIPIGNRDMTINAEFYRTQFINQIIADFESIREVKFYN
                     LDGKSYSNTYQIEASYTILKGLDATVAFRYNDVKQTIGGKLLEAPLTNRFKGLLTMSY
                     KTPLEKWQFDFTTQLNGPGRIPSTEENPAEYRLETEFPSYYIMNAQITKYFKFWEIYL
                     GVENIFNFVQKNPIIAATSPYGEYFDSSLIWGPIHGRKFHIGIRFALEKKENN"
     gene            6091..6576
                     /locus_tag="PKN53_07125"
     CDS             6091..6576
                     /locus_tag="PKN53_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOD88757.1"
                     /translation="MKKSILVLTMMFLAIVVVNAQDKNVKQVKKADSKISEVVLNCKM
                     DCGNCAEKVKKQLSYTKGVTAVEVNHETDLVVVKYRHDKTDVDKLIASLGEIKYQASV
                     YKPQSKDGKHAGCAGAKASGCPKATSGCPGAKAAGCAGDKTQLQGVSKDQRVTKEVEK
                     K"
     gene            complement(6767..8446)
                     /locus_tag="PKN53_07130"
     CDS             complement(6767..8446)
                     /locus_tag="PKN53_07130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOD88758.1"
                     /translation="MKNLNKILKTLKANISSVLIVLMLALSSFTFTTDKDINFETVKN
                     LDIFYSLYKELNLYYVDPIEPGEIIKTGIDAMLKSLDPYTVYIPESKIEDVKFMTTGQ
                     YGGIGSVIRKNGDYVVIIQPYKDSPSDKAGLIPGDIILEINGKNMKGASSEEVSELLR
                     GEPNTKVNLKIKRFGEDKNLDFELNREQVKLNSVPYYGMLEDGIAYISLSDFTNTAYK
                     EVKEAYDSLRTANNLKGIIIDLRSNPGGLLIEAVKICNMFIPKGEVIVSTKGKVAQWD
                     NVYKASVEPIDKDIKIAVIVNSSSASASEIVAGAMQDLDRGVVVGQRTFGKGLVQTTR
                     ELSYNGVLKVTTAKYYIPSGRCIQAIDYSNRNEDGSVGNIPDSLVSEFKTKNGRKVYD
                     GGGVKPDIVIQPEYLSNIAASLLIKNLIFDYSVYYKFKNPKIASVDKFVFSDADYNDF
                     VNWVQDKDFDYTTKSEDHLNQLIESAKTEKYYDDVQGEFEILRQKLAHDKNKDLQVFK
                     DEIKELISQEIVSKYYYETGPLIHNLHKDPEIKEAVRILKDDKEYKAILTP"
     gene            complement(8421..8831)
                     /gene="rnpA"
                     /locus_tag="PKN53_07135"
     CDS             complement(8421..8831)
                     /gene="rnpA"
                     /locus_tag="PKN53_07135"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOD88759.1"
                     /translation="MSKLKNNLKRKSFLKLKSEIQSLFNEGKKINTSSFRLLYHFELN
                     SQEYGLKLFVSAPKKLLKSAVKRNTAKRRFKEAFRLNCHHLKEISEKHKINIFVGIIH
                     NNSNLIEYSDIEKNLILSLQKIYNEIYEKFEQNS"
     gene            complement(8853..9596)
                     /locus_tag="PKN53_07140"
     CDS             complement(8853..9596)
                     /locus_tag="PKN53_07140"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005682536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOD88760.1"
                     /translation="MKIKSVLISQPKPETEKSPYFDISKKYNVKIDFRPFVQVKGIPA
                     KDVRRSKVDLCSHTAIIFNSRTAIDHYFRVLNELKININDEWKYFCYSESIALYLQKY
                     ITYRKRKIFFGNGTFNDLMESIKRFPEENYLVPLSEVHKPEIPIGLDKANIKYSKAIL
                     YKTVSADLSDLEDINYDVLVFFSPAGVNSLLHNFPNFDQNGICIGAFGAATAKTVKDL
                     KLRLDLCAPSPESPSMTAALEDFIKTRNK"
     gene            complement(10334..11245)
                     /locus_tag="PKN53_07145"
     CDS             complement(10334..11245)
                     /locus_tag="PKN53_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88761.1"
                     /translation="MTENTPEINFKKKDRLTKILIILITFMIVVIGILGFLLYDVSKQ
                     KQEIIIKTNEIVVEKDHLREQLTDLLDDYSQLSSTNDSLNAEIEREREHIAELIKELD
                     GIKNYNYSIQRKYEKELSSLRKIMRDYVYQIDSLDQLNKHLMAENINIKDQHSRIKNE
                     MDEVVEKNDELELLISGASVVKTSHISIKFLNRRGKETTKSRRVERLQTNFTLVANDL
                     AESGPRKVYLRIIRPNGYTFSSGQTFDFGSKKLTYTAYRDIVYEKQNLDVVIYYDVNE
                     TLEIGKYIVELYMDENKIGESFFNIEK"
     gene            11698..13986
                     /locus_tag="PKN53_07150"
     CDS             11698..13986
                     /locus_tag="PKN53_07150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD88762.1"
                     /translation="MENENQHIRDIAENKKNDFEWSLVFNTIEHPIFILSADGIVLEV
                     NKAAIKLFGVNSKNEIIGKKCCEKFSNFASIPEFCPGKTVETYSRTVECEVNKQTYIV
                     SCTPIYDKSNKFIKTIHIVTNISHLKYIEENLHENEERLSLLIANIPGVAYSCLFDRQ
                     WSMKFLSEEFKNLTGYDIADIINNKIKSYSDIIHIDDQERVWTTISESIAKKEPFLLE
                     YRIITKDNTIKYVWEKGKLVLRNKEHYIEGVIFDITERKEAEINLKNSEEKYRLLVEN
                     QNEVIFKFDRHGNFTYLSKTFYKVFSRGEDEKFADRNFMEFVLKEDQERILNFVSSLK
                     DAPHSGYIEHRILTNKGWRWYGWSCKAIIANTINEIIAVGRDITERKIIEDELKKETE
                     RIEEKEVNRTIFIANIIHEINPLVNNLVKYSELLNSKDVSNKEKEEFSKLINSSSKQV
                     SNIFNKFIEINQLESSNTKLEKEYFNISDLIDELAICYRPLAEIKNLEFVVLKPAQKD
                     IKLYTDKQKLIQILNNLISNAIKFTSFGNVKLGYRIEENKIKIFVADTGIGIESTNHK
                     MIFERFTQIKSDNQKFEGSGLGLSIVKELCDMLGTEIFVNSSLRHGSEFSFYLDYASS
                     EKDYAKDTESNLDNDLSELNILITEDDRINYLLLKTILNKAGANTLEAQTGSEAIEHV
                     KTFDDKIDVILMDINLPGLSGSDALLEIKKINNKIPVIACTAYTQNEDAQKLLNQGFD
                     DYIAKPINRNELLNIIKRNVVG"
     gene            complement(13983..15506)
                     /locus_tag="PKN53_07155"
     CDS             complement(13983..15506)
                     /locus_tag="PKN53_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOD88763.1"
                     /translation="MNVSIELFVLVISVLIFISLLVGKMGSRFGVPTLLLFLLIGVLF
                     GSDGVGLEFDSPKIAQVVGVVALNIILFSGGMDTRMSEIKPIAVQGFILATIGVMLTA
                     LITGVFIYWLTSNFFNAVTFSLLESLLLASVMSSTDSASVFSILRSRNLALKENLRPL
                     LEFESGSNDPMAYMLTIVLIQLIKSPEIDTWAAVFMFFKQLILGGLAGFLLGKYVFVR
                     IINKINLENDALYSVLLFTLMLFLFGFTTFIDGNGYLAVYIGGLFVGNSRFVHKRSSL
                     KFFDGMTWLVQIVMFLTLGLLVNPSELLPIAGVGILVGLFMIFVSRPITVFACLLPFR
                     KMTIRAKSYVSWVGLRGAVPIIFATYPWVAEISHAKTIFNIVFFITIISLLVQGTSVS
                     IMAKWLKISEDAPPKRKLKKFDVEIADEIKSFMSEITIKEEYLANGSRLMDLSVPDNT
                     LIVLVKRDDQYFVPRGNTELFVGDSILVITDDEEALEITHKVMKGKTNNKDKLPKQS"
     gene            15655..16986
                     /locus_tag="PKN53_07160"
     CDS             15655..16986
                     /locus_tag="PKN53_07160"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOD88764.1"
                     /translation="MTTLIKNALLINENNSQIASLLIEDERIKKIFSENENLPHADKI
                     IDATGLWLLPGIIDDQVHFREPGLTQKADIASESAAAVAGGLTSFIEMPNTNPATITN
                     QLLQEKFDIAKNSSYINYSFMLGATNDNIEEIRNINKSEVAGIKMFLGSSTGDLLVDD
                     DEAIENILSSTKSIVAVHCEDNEIINANLKKFEAKYGSNIPSKFHSQIRSEDACYKSS
                     SKAIEIAKKTGGRLHIFHLSTEKELELLSNEPLSQKNITAEVCVHHLWFSDKDYERLG
                     NKIKWNPVVKTEKDRLALIQALKDGKIDVVGSDHAPHLLSEKMQDYTKAPSGGPMVQH
                     TLLVMLELAEQKYFSKEEVVKFMCHNPAELFGIVDRGFVREGYFADLVLVNPNIPTKV
                     EKENLLYKCKWSPLEGETFKHSVQTTFVNGKIVFDKGKLTGIKSAKQLRFS"
BASE COUNT         6223 a         2283 c         2650 g         5911 t
ORIGIN      
        1 aaatggatat tattatttta caataaacaa tgtccacttt tttgctgata caactaaaaa
       61 tgagcatgaa gttgatttaa aaatttcatt gagaaaatca tttgcagaag atgatattta
      121 taattcagaa ggatttattt atcaaacgat aagaaacata aacatttatt ctgattatga
      181 tccaaaacaa gcagcaaaca atacggaaca atatttggct tcttttaaca aacaaaatat
      241 aaacggaata aatctatttt ataaagaaaa actacaatta aaaccatcag taattttaca
      301 gtcttgtttt ttaaatttag gtgatagata taatattgta aatgttgaga aaacacatag
      361 ccatcttgcg aacttaaagc aatttaaaat aataaacata aaattaaatg cttctgaaga
      421 tgtgttttta gatagccaaa aagaaaggtt tttagatgta aatatttatt taacaccatt
      481 aaaacgccaa aactattctt tagagcttga aggcaataat acttcgggaa atattggtat
      541 gggaggagcg ataacttata ataataaaaa tattgcaaaa agagctcaaa tgttttctgc
      601 taaagctatg ctttcttttc aaactttaaa ggcagaagaa aacacagaaa ggctaaaatt
      661 tttcaacact ttagaatatg gtttcgactt aggagtacat attcctagac tattagtgcc
      721 tttttgggat aattatgagt tttcaaaaaa ccatattcct aaaacacatg taacaatgtc
      781 tttaaactat cagcaacgtc ctgattatac acgttacatt gcaaaatctg cttttggtta
      841 tgtgtggaaa ggtggcaaaa ataattatat aactcatatg gtaaatccaa tagaattata
      901 ttctgttaaa attttagact tcaatcccga gtttcaaaaa caaatagaaa atttatatat
      961 aaaatattca tatcaagacc aactgctttc tgttattagc tataatttta tttttaataa
     1021 tcaaagttta accaagtctc gcgatttttc atacctttgg ttaaatcttg aaacaagtgg
     1081 taacttgcct tacttggcct acaaaggatt aaacatagaa ccagttgatg gctcatacaa
     1141 gtttttagga ttagaatttg ctcaatatgc caaaatagat ttagactata gatactatca
     1201 agtttttgat gaaaaacata gtttagttta cagaggcttt gttggggtag gttttcctta
     1261 tggaaattct acttcaggac tgccatttat aaaaaaatat tttgttggcg gagcaaacga
     1321 tttgcgtgct tggcgtgtaa ggtctatagg acctggaact tattcaaatc caaatagaaa
     1381 ttacgaccaa attggagata tgaaatttgt ttttaatctc gaatatcgct tcccaattat
     1441 ttcacttgtt aacggagctg tgtttttaga tgcaggaaat atttgggcgg ttgataaaaa
     1501 tgataaccgt gcaggtgctt tatttaactt gtacaggttt tataatgaaa ttgctatagg
     1561 aacaggttta ggagcacgtc ttgacctttc gttttttgtt attagatttg attttggaat
     1621 tcctattcac gacccaagtt tagcacaggg cgacagatgg ctttcggcat ttaaagattt
     1681 taaattaaga aatttcacaa tgaattttgg tataggttat cctttctaga tattgatatg
     1741 attaaaaaaa cattttttat tttagtttta gtttgctcgc aattttgttt gttttctcaa
     1801 aaccacgtgt ttttaggaat aaatccttct gtaaatttta atagagacta tccttctgga
     1861 gcttttgata tgaatgttct tccattaaca attgaaattc caataataaa caatgttgac
     1921 ataagactta tttccaaatt aaattatggt tttagaacta ctggtggagc tttagttggt
     1981 gtgggcgggg aagtgggttt accagtttat attcacagag gcgaatttga aaataatatt
     2041 cccacaggat tttatatttc cccaggattc ttgtatttga aaaatgtttt ttataaacat
     2101 agccataaag caatatttat cgagccaggc tataattttg catttaaaaa taaatttggt
     2161 ttatgccttg gtttgcaata tggtcgcaaa ttccttaatt ataccgatgg aagtaaatta
     2221 caagctaatc attttgggct aaagataatt ttggggtttt ggttgtaaat gagatctttc
     2281 aataaatttt ttcttatttt gtttttattt gtttatttat tcgaaaagtt ttaattttgt
     2341 aataatagaa aattaataaa taaaatttat gttcatagct aatcctatat acgatacagt
     2401 ttttaaatat atgatgagtg atactaaagt tgcaaagatt tttttatcag ctatcataga
     2461 ggaaaagatt gttgaattag attttgcttc aacagagcgt tctttaaaac ctggtgctaa
     2521 acgaagtgat ataaacagag aattagaaga attggtcgtt tgtcgttttg atttcgctgc
     2581 aaagataaaa actggcagtg gagaatataa aactgtaact atagaattgc aaaaagctaa
     2641 atttgcaact gatataatgc gttttagaag atatttagga gttatgtatc aagatgcaga
     2701 caatacttct gacaaacagc gactaaaagc aaggcaaatt tactgtattt atttattaaa
     2761 ttatgatata ggtttgtcaa acagtcctgt gataaaagtt gaccatattg ttagtgacca
     2821 agcaacaggc gaagagttaa gtaataaaaa tgaatttatt caaagtttaa atcacaaatc
     2881 gtggattgtt cagattaggc aattaaagga aaatcgaaga aatgatttag agaacttgtt
     2941 gagcatattc gaccaagaca atattacaaa agacaaccat attttgaatg taaattctga
     3001 aaatttttca gaagagcata aacatattgt tcgcaaattg caagaagcct atgcttcaag
     3061 tcaaatacga gatgaaatga taatggaaga cgactacttt aaagaattag aagtaaaaga
     3121 tgatataatt gaagaacaaa agaaaaaatt agttaaaaat gccaagacaa tagctgaaaa
     3181 agacaagaca atagctgaaa aggataaaga aatagctgaa ttaaagagac tttcagaatt
     3241 aaaaaataaa gaaaaataaa attcatatca atcaacaaac aatattgtct tttgtttaaa
     3301 ttgaattaaa tatatatttt tgcagtgaaa tgaacttgtt gaagaacata tttattctat
     3361 ttacaacact aattttgttt gtttcttcaa acggattttt tgttgaacaa tatttatgta
     3421 atgcttgcaa tgaggaacat agtgaaatta cattttttga atttggagaa tattcacaca
     3481 accataaaca ttatactgct tgcgaccatt cacattcaaa ttgcttgtgt tctcaatctg
     3541 atgaagacca cttggagcat gcaaaagttt ctttctattc ttttgataaa ttattttttg
     3601 aatatgaact tgtgaaaatt ccacaaatta aatttcttga taattttaat ttacaagtat
     3661 ttccaataga tataaataag gaattacttt catttaatag ttttgttgaa acattattaa
     3721 aaatcccctc tttattcaca tatcagtctg taggacaaga tgtttgcacc ttgatttctg
     3781 tttttagatt atgattttta cgattttatt agtttaaaat tagtattaat caaataaaaa
     3841 cagaatgaaa tatgaaaaaa atatatagca ttatttttaa tgtgttattt gtattaacac
     3901 ttaatgcaca agtctctgga attgtttatg acaactcgga taaaaagcca atgtttggag
     3961 taaatatatg gtggaaaaat acaaatattg gtacaacaag cgatatatat ggtaaatttg
     4021 agttgtcaaa acctaaggaa agtaataaat tgatttttag ctttgttggc tataatacag
     4081 acacaataga agttttaaaa aacaatcaaa aattagaagt ttttttaaat agcaatataa
     4141 ttttagatga ggtaattgtc ggtgaacgta ggtcaggaac tcattattca cgtatggagg
     4201 taggaaatgt tcaaacaatt tctggtgcag aattgtgtaa ggcagcttgt tgtaatttgt
     4261 cggaaagttt tgaaacaaat gcttctgtag atgcttcgta tagcgatgca acaacaggag
     4321 caaagcaaat aagacttttg ggcttggctg gaaaatatgt tcaaatgatg acagaaaata
     4381 ttcctaattt atacggattg tctcagcctt atgctttagg ttatattcca ggaccttgga
     4441 tggagtctat tcaagtttca aaaggaactt cggcggtaat aaatggttat gatgcaatta
     4501 ctggacaaat aaatatcgaa tataaaaaac ccaaaacttc tgatgctctt tcttttaatc
     4561 aattatttag ttcatctgga cgagcagaaa caaatataga tgcatcattt attttaaatc
     4621 caaagtggtc aacaatgatt tttgctcatt ctcaacatga ttttttagaa attgatgaaa
     4681 ataaagatgg ttttatagat atgccaaaaa tgaggcaaaa tattttgttt aatcgttggg
     4741 attatttagg taagtatttg acaattagat ttggagctca atatattgat gaggtaagaa
     4801 aaagcggaca aacttctaaa tttttaaaaa ataatgaaat tacaaatccc tacaaaatat
     4861 ttttaggaac aaaaagattt caaggttttt ataaaatggg ttatgtattt ccgaaaaaag
     4921 actataagag tttagctatg gtaactagtt ttacttatca cgaccaaaat tctatttatg
     4981 gcaaaacaaa ttttgatgca acacagcaat cggcatattt aaacattatt tggcaatcgg
     5041 cttttgcagg taacgaagag cataaataca atgcaggatt aagttttaaa tatgaaaatt
     5101 tggatcaact gcttaaagat tctacaatgt taaatcaaga aatagttcca ggaggatatt
     5161 ttcaatacac ggcgaacttt ccttttaatt tgcatttaat tgctggattg cgtgccgatt
     5221 atcataatca agaaggactt ttgataacac caagattaaa tataaaatat aatatcactg
     5281 gtgctttatc ttggcgggct tctgttggta aaggctaccg ttatgcaaat gtattagccg
     5341 aaaataatta tttattggct tcgtctcgaa aaattataat tgctccagat ttaaaattag
     5401 aagaagcgtg gaattttgga agtaatatta cttggtatat tcctattgga aatagagata
     5461 tgacaataaa tgcagaattt tacagaactc aatttataaa tcaaattatt gcagattttg
     5521 aatcaatacg cgaagttaaa ttttataatt tagacggaaa atcttattca aatacttatc
     5581 aaatagaagc aagttatacc atattaaaag gcttagatgc aacagttgct tttcgatata
     5641 atgatgtaaa acaaacaata ggcggaaaat tacttgaagc accattaaca aatagattta
     5701 aaggcttatt aactatgtct tataaaactc ctttggaaaa atggcagttt gattttacaa
     5761 cacaactcaa tggacctggc agaattcctt caacagaaga aaatccagca gaatatagat
     5821 tagaaacaga atttccatcg tattatatta tgaatgctca aataacaaaa tattttaaat
     5881 tttgggaaat atatcttggt gttgaaaata tttttaattt tgtacaaaaa aatccaatta
     5941 ttgcagcaac aagtccatat ggagaatatt ttgattcatc gttaatttgg ggaccaatac
     6001 atgggcgtaa atttcatatt ggaataagat ttgcattaga gaaaaaagaa aataattaaa
     6061 atttttaata ataaattaaa tttaaaaagt atgaaaaaat caattttagt tttaacaatg
     6121 atgttcttgg ctatagtagt tgtaaatgct caagataaga atgtaaaaca agtaaaaaaa
     6181 gcagattcta aaatctcaga agtagtttta aattgcaaaa tggattgcgg aaattgtgct
     6241 gaaaaagtta aaaaacagtt gtcatataca aaaggagtaa cggctgttga agttaatcac
     6301 gaaacagatt tagtggttgt aaaatatcgc cacgacaaaa cagatgttga taaacttatt
     6361 gcttctttag gagaaataaa atatcaagcg agtgtttaca agccacaaag taaagatgga
     6421 aaacatgctg gttgtgctgg tgctaaggct tctggctgtc ctaaagctac tagtggttgt
     6481 cctggtgcta aagctgctgg ttgtgctggt gataaaactc aacttcaagg agtaagcaag
     6541 gatcaacgag taactaaaga agtagaaaag aaatagttgc aaagcaaaga ctgtcaagct
     6601 attcaatccg ctcgctcgga tttgtaatcc gagtgtaaca cttccaacac taggattaca
     6661 aatccgagcg agcatcatgg gtgttgagag cgagcatcat gggtgttgag agcgagcatc
     6721 agtattcaat tgaaatttgc ggttggatat ttttaagaca gtttttttat ggagttaaaa
     6781 tagccttata ctctttatca tcttttaaaa ttctaacagc ttctttaatt tcggggtctt
     6841 tatgcaaatt atggattaaa ggtccggttt cgtagtaata tttacttaca atttcttggg
     6901 aaattagttc ttttatttcg tctttaaaga cttgtaaatc tttgttttta tcgtgggcta
     6961 atttttgtct tagtatttca aattcgcctt gtacatcatc gtaatatttt tctgtttttg
     7021 cactttcaat taattgattt aagtggtctt cactttttgt tgtatagtca aaatctttgt
     7081 cttgaaccca gtttacaaaa tcattatagt ctgcatctga aaacacaaat ttatctacac
     7141 tagcaatttt aggattttta aatttataat aaacggaata atcaaaaatt aaatttttaa
     7201 taagcaagct agcagcaata ttgcttaaat attcaggttg aattacaata tctggtttta
     7261 caccaccacc gtcatagact ttacgtccat ttttagtttt aaattctgag actaaagaat
     7321 ctggaatatt tccaacactt ccgtcttcgt ttctattaga atagtctatt gcttgaatac
     7381 atcttccgct ggggatataa tattttgctg ttgtaacttt taaaacgcca ttatagctta
     7441 attctctagt tgtttgtact aatccttttc cgaaagtacg ttgtcccact actacccctc
     7501 tatctaaatc ttgcatagca ccagcaacaa tttctgaagc cgaagccgaa cttgaattta
     7561 caattactgc tattttaata tctttatcaa taggctcaac acttgcttta tacacattgt
     7621 cccattgagc aacttttcct tttgtgctaa caattacttc gccttttgga ataaacatat
     7681 tacatatttt tactgcttca atcaataatc caccaggatt gcttcgtaaa tcaatgatta
     7741 ttcctttgag attatttgct gttcttaaac tgtcgtatgc ttctttaact tctttatagg
     7801 cagtatttgt aaagtcgctt aacgaaatat atgcaatacc atcttctaac attccgtagt
     7861 aaggaacaga atttaattta acttgctctc tatttaattc aaaatctaag tttttatctt
     7921 cgccaaaacg tttaattttt agatttactt ttgtattagg ctctcctctt agtaattcag
     7981 aaacttcttc agagcttgca cctttcatgt tttttccatt aatttctaag attatatcgc
     8041 caggaattaa acctgcttta tcagaaggag aatctttata tggttgaata ataaccacat
     8101 aatcgccatt ttttctaatt acagagccaa taccgccata ttgacctgtt gtcataaact
     8161 taacatcttc aatttttgat tcaggaatat aaactgtata tgggtctaaa cttttaagca
     8221 ttgcatcaat acctgtttta ataatttctc caggttcaat aggatcaaca taataaagat
     8281 ttaattcctt atataaagaa taaaaaatat ctaaattttt aactgtttca aagtttatat
     8341 ctttgtcggt tgtaaaagta aatgaactta aagctaacat taaaacaata agcactgagc
     8401 ttatattagc ttttagtgtt ttaagaattt tgttcaaatt tttcatatat ttcgttataa
     8461 attttttgta aagataagat taaatttttt tctatatcac tatattcgat taagtttgaa
     8521 ttattatgaa taatacccac aaaaatatta attttatgtt tttcagaaat ctcttttaga
     8581 tgatgacagt ttaatctaaa agcttcttta aatcttcttt tagcggtatt tcttttaacg
     8641 gctgatttta aaagtttttt tggggcagaa acaaataatt ttaaaccata ttcttgagag
     8701 ttgagttcaa aatgatataa aagcctaaaa cttgaagtgt taattttttt cccttcatta
     8761 aaaaggcttt gtatttctga ttttagcttt aaaaaagatt ttctttttaa attatttttg
     8821 agtttactca ttacaaatca tatactcaac aactatttgt ttcttgtttt aataaaatct
     8881 tccaaagcag ctgtcattga tgggctttct ggtgaaggag cacaaaggtc tagtcttaat
     8941 tttaagtctt ttacagtttt tgctgtagca gcaccaaatg caccaataca aattccgttt
     9001 tgatcaaaat ttggaaaatt atgtaataat gaattaacac cagcagggct aaaaaatact
     9061 aaaacatcat aattaatgtc ttccaaatca gataaatcag cacttacagt tttatataaa
     9121 attgctttgg aatattttat attagcttta tctaaaccaa taggaatttc tggtttgtga
     9181 acttcgctga gtggtacaag ataattttct tcaggaaatc ttttaatact ttccattaaa
     9241 tcattaaaag taccatttcc gaaaaatatt tttcgtttac gataagtaat atatttttgc
     9301 aagtaaagag ctatagattc tgagtagcaa aagtatttcc attcatcatt tatattgatt
     9361 tttaattcgt ttaaaactct aaaataatga tctattgcag ttctactatt aaaaataata
     9421 gcagtatgtg aacacaagtc aacttttgaa cgcctgacat cttttgcagg aattcccttt
     9481 acttgtacga aagggcgaaa atcaatttta acattatatt ttttagaaat atcaaaataa
     9541 ggtgattttt ctgtttcagg cttaggctga gaaataagta cgctttttat cttcattctt
     9601 cttttgttat cattaagtta ctaccacata cctactaatt tacaaacaat gagtaaaggt
     9661 aaaatttcca aagtgcaaaa atacaaaatt aagaaatgat ttaaaaattt tattttgaat
     9721 aaataaataa aaaatgaaat tatttttatt aaaaaaatta ttgctaatat caaaatggct
     9781 gcaaaaacaa ggtgtgactg catttttgta tttgcaaaag ggaataataa atataaaggt
     9841 gataaaaata aggataggac tttaatatta ttaagcctta ttaatgtgca atctatagca
     9901 taagatttca tattggttaa atttcctatc agcattaaat ttgtttttgt aaataaaaag
     9961 taaaaaaaca cagcaaatat taaaatgcaa atgagtataa aactatttgt tatattaaat
    10021 ataggaaatt taaaataagt tataatcatc ataagtgtgg cagatatgtt taaaataggt
    10081 atgctaatag ttacaatatt tagaaatgcg ttagagttgg acttttgttt tgttttttgc
    10141 aaatcttcaa atctgagtgt tgatttaatt aaattttgca aataattttg attagttaat
    10201 tttgttataa tgattaatat aaaaactaca aatgcaataa tgcttaaaat taaataatta
    10261 tcattagaat ttctttctat tatgggcatg ctaatatcaa gaagttctaa ataagcatta
    10321 gctaacataa actttatttt tcaatattaa agaaactttc tccgatttta ttttcgtcca
    10381 tatataattc aactatatat tttcctattt ccaaagtttc gtttacatca taatatataa
    10441 caacatcaag gttttgtttt tcgtaaacaa tatcgcggta agctgtatat gttagctttt
    10501 tagaaccgaa atcgaaagtt tgtccactag aaaaagtata tccatttggt ctaataattc
    10561 ttaaatatac ttttcgagga ccactttcag ctaaatcgtt agctactaaa gtaaaattag
    10621 tttgcagtct ttcaactctg cgcgattttg ttgtttcttt tcctcttcta tttaaaaatt
    10681 ttatgcttat atgagatgtt tttactacgc tagcaccaga aattagtaat tctaattcgt
    10741 cgtttttttc aacgacttca tccatttcat tttttattcg tgaatgttgg tcttttatat
    10801 ttatgttttc tgccattaaa tgtttattta gttggtcaag actatcgatt tggtaaacat
    10861 aatcacgcat aatttttctt aaagaagaaa gctctttttc atattttcgc tgaatactat
    10921 aattgtaatt tttaataccg tcaagttctt ttattaattc tgcgatatgt tctctttcgc
    10981 gttcaatttc agcatttaaa ctgtcgttag tagagcttag ttgagaataa tcatctaaaa
    11041 ggtctgttaa ttgttctcgt aaatggtctt tttcaacaac aatttcattt gtttttataa
    11101 ttatttcttg tttttgtttt gaaacgtcgt aaagtaaaaa tcctaaaata ccaattacaa
    11161 caatcataaa tgtaattaaa ataattaaaa tctttgttag tctatctttt ttcttgaaat
    11221 taatttcagg tgtattttct gtcataaata tatttttttt gcaaaattat aaaaaaaagc
    11281 tgtgagtgat aacaaagttt tgttttttat acacaaagtt atgtgagttt ttagttttaa
    11341 accacaaaag acacaatcag ttaaaagtta aaagcgatgc actgaacgta gttgaagtgt
    11401 taaaagttga accgagttta cgagctcacc gaaggtaaag taggctgacg cgtctattat
    11461 ttcgcagagt tcgcaagctc actcttttta gttgtcgagt ttttagtttt tagtattgag
    11521 ccgagcttgc gataaaaatg ttataacata taagaaaaaa cctaaagtag aaatatatac
    11581 tgcactagca aagcatgtag caactttata aaacgtttaa attttggaat tttatttctt
    11641 ataaattcta tataatgagt aaaaattatt aattttgctt ttaattatta ctaatcaatg
    11701 gaaaacgaaa atcaacacat tagagatatt gcagaaaata aaaaaaatga ctttgaatgg
    11761 agtcttgttt ttaatacgat agaacatcca atttttattt tgagtgcaga tggtattgtt
    11821 ttagaggtaa ataaagcagc tataaaactt tttggagtaa actcgaaaaa tgaaattatt
    11881 ggtaaaaaat gttgtgagaa attttctaat tttgcttcta taccagaatt ttgtccaggt
    11941 aaaaccgttg aaacatattc tcgaacagta gaatgtgaag ttaataaaca aacatatata
    12001 gtttcttgta ctccaattta tgacaaatca aataagttta taaaaaccat tcatattgta
    12061 acaaatattt ctcacctaaa atatatagaa gaaaatttac atgaaaatga agaaagatta
    12121 agtttgttaa tagctaatat tccaggtgtt gcctacagtt gtttgttcga taggcaatgg
    12181 tctatgaagt ttttatctga agagtttaaa aacttaacag gatatgatat tgctgatatt
    12241 ataaataata aaatcaaaag ttattccgat ataatacata ttgacgacca agaaagggtt
    12301 tggactacta ttagtgaaag tatagcaaaa aaagaacctt ttttattaga atatagaata
    12361 attacaaaag ataatacaat aaaatatgtt tgggaaaaag gaaagcttgt tttacgaaat
    12421 aaagaacact atatcgaagg cgtaatattt gatattactg aaagaaaaga agcagaaata
    12481 aatttaaaaa atagcgaaga aaaatatagg cttttagtag agaatcagaa tgaggtaata
    12541 ttcaaatttg acagacatgg aaattttacg tatttgagta aaacttttta taaggttttt
    12601 tcaagaggtg aagatgaaaa atttgctgat agaaacttta tggaatttgt tcttaaagaa
    12661 gatcaagaac gaattcttaa ttttgtaagt tctttaaaag atgctccaca ttcaggatat
    12721 attgaacata gaattttaac aaataaaggc tggaggtggt atggctggag ttgtaaagca
    12781 attattgcta atacaataaa tgaaattatt gctgttggaa gagatattac tgaaagaaaa
    12841 attatagaag atgaattaaa aaaagaaaca gaaagaatag aagaaaaaga agtaaataga
    12901 acaatattta tagcaaatat tattcatgaa attaatccat tagtaaataa cttagttaaa
    12961 tatagtgaat tgcttaacag taaagatgtt agcaataagg aaaaagaaga gtttagcaaa
    13021 ctaataaatt cttcatcaaa acaagtttcc aatattttta ataaatttat tgaaatcaat
    13081 caattagaat caagcaatac taaacttgaa aaagaatact ttaatatttc tgacctaata
    13141 gatgaattgg caatttgtta tagaccttta gccgaaataa aaaatcttga atttgtagtc
    13201 ttaaagccag ctcaaaaaga tattaaactt tatacagaca agcaaaaact tatacagatt
    13261 ttaaataact taataagtaa tgctataaaa tttacaagtt ttggtaatgt taaacttgga
    13321 tatagaatag aagaaaataa aattaaaatt tttgtggctg atacaggtat tggtatagaa
    13381 tcgactaatc ataaaatgat ttttgaaaga tttactcaaa taaaatcaga caatcaaaaa
    13441 tttgaaggca gtgggcttgg cttatcaatt gttaaagaac tttgtgatat gcttggaaca
    13501 gaaatttttg taaactctag tttacgacat ggttcggagt ttagttttta tcttgactat
    13561 gcaagctcgg aaaaagatta tgcaaaggat acagaatcaa atttagacaa tgatttatca
    13621 gaattaaaca ttttaattac tgaagatgat agaataaatt atttattatt aaaaactatt
    13681 ttaaacaaag caggggcaaa cactttagaa gcacaaacag gtagcgaagc tatagagcat
    13741 gtaaaaactt ttgacgataa aatagacgtt attttgatgg atataaattt accaggcttg
    13801 agtggtagtg atgctctttt agaaataaag aaaataaata ataaaattcc agtaatagct
    13861 tgcactgctt acactcaaaa tgaagatgct caaaaattac ttaaccaagg ttttgatgat
    13921 tatattgcta agccaattaa tcgtaatgag ttattaaata ttataaaaag aaatgtagta
    13981 ggttaggatt gcttaggtag tttatcttta ttatttgttt tacctttcat aactttatgt
    14041 gttatttcca aagcttcttc gtcgtcagta attaccaaaa ttgaatcgcc aacaaaaagt
    14101 tctgtatttc ctcgcggaac aaaatactgg tcgtctcgtt ttactaaaac aatcagcgta
    14161 ttgtctggca cgcttaaatc cattaatctt gaaccatttg ctaaatattc ttcttttata
    14221 gttatttcgc tcataaaaga ttttatttcg tcagcaattt ctacatcgaa ttttttcaat
    14281 tttcttttag gcggagcatc ttctgatatt tttagccatt tagccattat gcttactgaa
    14341 gttccttgta ctaatagcga aataattgtt ataaaaaaca caatgttgaa aatagttttt
    14401 gcatgtgata tttctgctac ccatggatat gttgcaaata taataggtac tgcaccgcgt
    14461 aatcctaccc aagaaacata gctttttgct cttattgtca tttttctaaa tggtagaagg
    14521 caagcaaaaa ctgttattgg acgtgaaaca aaaatcataa ataaaccaac aagaattccc
    14581 acacctgcta ttggtagcaa ttcagaagga ttaactaaca agcctaatgt aaggaacatc
    14641 actatttgta ctaaccaagt cattccgtca aagaatttta gcgaactacg tttgtgtaca
    14701 aatctactgt ttccaacaaa taaaccacca atatatacag ctaaataacc gttgccatca
    14761 ataaaagtgg taaatccaaa taaaaatagc attagggtaa aaagcaaaac agaataaaga
    14821 gcatcgtttt ctaggttaat tttgttgata atgcgaacaa atacatattt ccctaataag
    14881 aaacctgcta atccacctaa aattaattgt ttaaaaaaca tgaacacagc tgcccaagtg
    14941 tctatttctg gagattttat aagttgaata agtacaatag tgagcatata cgccataggg
    15001 tcgttacttc cgctttcaaa ctctaataaa ggtcgtaaat tttcttttag tgcaagattt
    15061 cgcgaacgta gtatagagaa tacggaggca gaatctgtag atgacatcac actggcaagc
    15121 aacagagact ctaataacga gaatgttaca gcattaaaga aattgctagt tagccaatat
    15181 ataaaaactc ctgttattaa agctgtgagc attacgccaa tagtagcaag tataaatcct
    15241 tgtaccgcaa ttggttttat ttcagacatg cgggtatcca ttccacccga aaataaaata
    15301 atgttaaggg caaccactcc aacaacttgg gctatttttg gcgaatcaaa ctcaagacca
    15361 actccgtctg acccaaacaa tacgcctatt aataaaaaaa ggagaagggt aggcacgcca
    15421 aaccgacttc ccattttgcc taccaaaaga ctgataaaaa tgagaactga aatgactaat
    15481 acaaataatt ctatcgagac attcataatt ttgcaaaact aatatttttt aataattatt
    15541 ttaataaaat ttttgaaaaa tacttttgtg catattattt tcggtaaaaa tctttttata
    15601 attatgaata atgtattttt gtgtaaaatt taatgatatt tctaataatg tgctatgaca
    15661 actttaatta aaaatgcttt attaatcaac gaaaataact cacaaattgc tagtttactt
    15721 attgaagacg aaaggataaa aaagattttt tctgagaatg aaaatttacc ccatgctgat
    15781 aaaattatcg atgcgacagg cttgtggttg ttgccaggga ttattgatga ccaagtgcat
    15841 tttcgtgagc ctggtttaac acaaaaagcc gatattgctt ctgaatctgc agctgctgtt
    15901 gcaggtggat taacttcttt tatagaaatg ccaaatacta atcctgcaac aataacaaat
    15961 caacttttac aagaaaaatt tgatatagct aaaaacagtt cttacatcaa ttattccttt
    16021 atgttaggag caacaaatga taatattgaa gaaataagga atataaataa atctgaagtt
    16081 gctggaataa agatgttttt ggggtcttca acaggcgatt tgttagtaga tgacgatgag
    16141 gctatagaaa atattttgag ttcaacaaag tcaattgttg cagttcattg tgaggataat
    16201 gaaataatta atgcaaatct taaaaagttt gaggcaaaat atggcagtaa tattccttca
    16261 aaatttcact ctcaaattcg ttcggaagat gcttgttaca aatcatcgtc taaagcaatt
    16321 gaaatagcta aaaaaacagg tggtcgctta catatttttc atttaagtac cgagaaagaa
    16381 ctagaattat taagcaatga gcctctttct cagaaaaata taacagctga ggtttgcgtt
    16441 catcatcttt ggttctctga caaagattat gaaagattgg gaaataaaat taaatggaat
    16501 cctgtggtaa aaactgagaa agatagatta gccttaatac aagccttgaa agatggcaaa
    16561 atagatgtag taggtagtga tcatgctcca catttattat cagaaaaaat gcaagattac
    16621 acaaaagctc cttcgggtgg tcctatggtt caacatactc ttttggtaat gttagaactt
    16681 gctgaacaaa aatatttttc taaagaagaa gttgtaaaat ttatgtgcca taatcctgct
    16741 gaattgtttg gaattgttga tagaggcttt gttcgagaag gctattttgc tgatttagtt
    16801 ttggttaatc caaatattcc aacaaaagta gaaaaagaaa atcttttgta taaatgcaag
    16861 tggtcgcctt tagaaggtga aacttttaaa cattctgttc aaactacttt tgtaaatggg
    16921 aaaatagtat ttgataaagg gaagttgacg ggaattaagt ctgctaaaca actgcgattc
    16981 agttaaaaat tgaaaaagtt tttagttttt actatgaaga tgaactaaga attaagaatt
    17041 gtgtttcgac ttcgctcaac acccgtt
//
LOCUS       DAOPNX010000055        16965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_4033_length_16965_cov_6.071614, whole genome shotgun sequence.
ACCESSION   DAOPNX010000055 DAOPNX010000000
VERSION     DAOPNX010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16965
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4033_length_16965_cov_6.071614"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1442)
                     /locus_tag="PKN53_07165"
     CDS             complement(51..1442)
                     /locus_tag="PKN53_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOD88765.1"
                     /translation="MFKPEVYKKRRESLKKDLQKGLYLFLGNNDTPMNYPANTYHFRQ
                     DSSFLYFFGLNLPGMAAIIDIDNDKEIIFANDVDIDDIIWMGPQPSVKELASKCSVFE
                     TYEFAKLSDYIKDALKKGREVSYLPAYKHDNMILLHSLLGISFQELKAKASLKFIKAI
                     VKLRNIKEEIEIQEIEKACDIGYKMHTVAMKHAYPGVVEREIAGIVEGISLSYGSVPS
                     FPVILTKNGQTLHNHSRDNVLQKGDLMLHDSGAQTPMFYASDNTRTVPVGGKFTQKQK
                     EIYQIVLNAINGSIKLMKPGKTYMDIHLQACEILANGLKELGLMKGNVQEAVALGAHA
                     LFMPHGLGHALGLDVHDMEDLGQTNVGYDDKVRPSEIFGTAFLRFGRELKPGHVLTNE
                     PGIYFIPELIDLWQKDKKFEEFINYSKVNEYRNFGGIRLEDDILITETGNRILGSKRI
                     PISIEEVEHTMLS"
     gene            complement(1572..1949)
                     /locus_tag="PKN53_07170"
     CDS             complement(1572..1949)
                     /locus_tag="PKN53_07170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD88766.1"
                     /translation="MKTNFYILILIFLCFGLNSQANTYYNYSNHNFSNFIFDNYPEVG
                     KISAPVPNPVNDYANFYYNLPEEQNGEIAIFDFTGIKVKSVQIQAGQGEIQIATYDLK
                     AGVYFACLIVEGKTLDSKKLIKK"
     gene            complement(2104..3261)
                     /locus_tag="PKN53_07175"
     CDS             complement(2104..3261)
                     /locus_tag="PKN53_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HOD88767.1"
                     /translation="MSTIAFVPVRSGSKSIKDKNIKLLNGKPLFFWVLNALQNASEIN
                     KIILATDSTNYAEIAKSFNFSKLEIYMRSAENASDTASTESVVLEYLEKANLNSQDIF
                     VLAQATSPLTKSEDFDNALRQFAYSGKNSMLSCVKSKRFFWDKDGNTINYDYRNRPRR
                     QDFDGMFMENGAFYINKVENILKDKNRLSGQIGIYEMHESTAIEIDEPIDWKIVEILL
                     AEQNAVKKAQISDIKLFMSDVDGVLTDAGMYYSENGDELKKFCTHDGMGIKLLKEKGV
                     KVGIITTEDRQLNRNRANKLKLDYHFHGETNKLETIKNLCKELNISLSEVAYIGDDVN
                     DFELLSAVGLAACPANSVEKIKNIPNIIHLTKKGGEGAVREFIEMILSVKS"
     gene            complement(3258..4133)
                     /locus_tag="PKN53_07180"
     CDS             complement(3258..4133)
                     /locus_tag="PKN53_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOD88768.1"
                     /translation="MKKYKEPKVIAEIGCNHKGDFEIAKELILLAKLSGADYAKFQKR
                     NNKELLTPEQYNSPHPNEMHAYGKTYGEHREFLEFSLEQHAELKKFCEANSIGYATSV
                     WDLTSAKEIASLKPDFIKIPSACNNHYEMLEYLRDNYSGYIQASLGMTTHEEEEALVK
                     LFEKNNSAKERLILFSCTSGYPVAFEDVSLLEIERIKSEYGNRIYDIGFSGHHLGIAI
                     DIAAYTLGASWIERHFTKDRTWKGTDHAASLEVPGLTKLVRDLKASHKALNYKRSEVL
                     EVEQIQRDKLKYRKR"
     gene            complement(4136..5035)
                     /locus_tag="PKN53_07185"
     CDS             complement(4136..5035)
                     /locus_tag="PKN53_07185"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOD88769.1"
                     /translation="MRDFLIKINSWFRLLAKTCASCVKILLFSKSTKQYKNLAKSEKD
                     ILILGNGPSLNSFIENHKEKIKNYNLIAVNHFAISEFYTELKPKYYIAIAHDLYLDDV
                     MPHFLEASNKLFNTLVEKTDWNLKFFITTEARKHQRWQEILKKNSNVEIIYINLTPID
                     GFKNFKFRNFDKLRGMPRPHNIMVPAIFTALNLQAKNILLAGVDHSWLKELQVDNENK
                     VLLFNQHFYDKEIKAKQFDYMGQRYVKLHEILYTLGEAFKSYHILKEYADYKQAKIFN
                     LTENSFIDAFERVNFEELNFKNK"
     gene            5200..5913
                     /locus_tag="PKN53_07190"
     CDS             5200..5913
                     /locus_tag="PKN53_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD88770.1"
                     /translation="MKKLFAEDIFGNCCKDFLAGDKDAKIVVNSDIAETDYLFAEYLF
                     REFDEMPEHEQKALLQARGDILDIGACAGSHSLYMQNMGYNVTSLDISQGCCEVMEKR
                     GLKNVVCSDVFSYSDKKFDTILLLMNGIGIAGNLPNLPKLLVHLKTLLKPGGKIIFDS
                     SDLQYLYLDDDGAYSIPLTDRYYGEMIYNLSYKDKKTGNFKWFFIDPYTIEAIANEVD
                     LKIQFLAWGNHYDYSACLY"
     gene            complement(5896..6057)
                     /locus_tag="PKN53_07195"
     CDS             complement(5896..6057)
                     /locus_tag="PKN53_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88771.1"
                     /translation="MSSQTYFARLDLAFLYELYKLYELYKLYELYELYKLYELYKLYE
                     LSTELIQTS"
     gene            complement(6162..9152)
                     /gene="secDF"
                     /locus_tag="PKN53_07200"
     CDS             complement(6162..9152)
                     /gene="secDF"
                     /locus_tag="PKN53_07200"
                     /inference="COORDINATES: protein motif:HMM:NF009585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HOD88772.1"
                     /translation="MRNKGAIVLVTVLLTLICLYYLTFTFAVKRVENKAEKYAESQFT
                     AMNLKVDEARQLEITDSLVKYYLDSVENLPALTLFSKHTYMDCKKRELNLGLDLKGGM
                     NVSLRISAEDLILSLANNPNDPTLLAALEETNKIIDGDDETDFITIFGDAYSKVKTPK
                     SSSLAALFSKISNSDKINAKSTDQEVIDYINAQYEDAIDNAFVVLRKRIDLFGVVSPN
                     IQKDIANRGVFHIELPGIKEPERVEDLLKQAAVLEFWETKDATEIRNNLAKVDDFLTQ
                     NEEEAIAIDTTNIVADTTNIDTTTNIVDAETNKSEQTQTRSITKRMVFNIGDRGVYGP
                     VLGYVKKADKDALINDLNADYLAGFFGNDVVFACGYKPVEGNIDYYEVVVLQRSKATG
                     GAALSGDVVTDARQEFDERTGQAHVSMSMNSAGATEWARITKENIGKSIAIVLDGQVY
                     SYPNVNDEIKGGHSSISGNFTLDEATDLANVLKSGKMVVSVSIISSEVVGPSLGQKAI
                     DSGFASFIIAFIIVLIYMIFYYNRAGLVANIALIANVFFIFGVLASIGAVLTLPGIAG
                     IVLTLGMAVDANVIIYERVREEIRAGKQIKSALVDGYKRSYPAILDGNITTLLVGIVL
                     AMFGVGPVKGFATTLIIGILTSLFSSLFITRLIFASLLKKEKKINFGNKFTNNLFTDL
                     NIGFIKNRKIFYTIAGSLAVISIILLFTKGLNQGVDFTGGRDYIVKFEQAVEPEEVAK
                     ALETVYEELPIVKTYGTKNQVKITTKYKIKEKDTDQLVDSLLYVGLKPLIGDDVSYEK
                     FNDDYKVRAQKVGPSVASDIVKKSILVVIIGLALMFIYILVRFTKWQYSTGATIALAY
                     NALLILGVFSLFDNILPFNMEVDQTFIAAVLTVIGYSINDTVVIFDRLREHQKLYKNR
                     PLDETINYAINSTIGRTVNTLMTTLLVVLAIFIFGGEVIRGFTFALLIGIATGAFSSV
                     CLASPIVYDLTERALRKQKKELKK"
     gene            complement(9354..9806)
                     /locus_tag="PKN53_07205"
     CDS             complement(9354..9806)
                     /locus_tag="PKN53_07205"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HOD88773.1"
                     /translation="MSKKFKTYQSKLHILNIEGGGNHVLIKAKVNKNKVNMIVDTGAS
                     STVFDLENPIFSNIDFENLETDNPSSGFNSVIEELYFGNIDSFSISWFKIKNFEAVFT
                     SMQHINYLYKNLNLPHVAGILGCDFLIENNAVLDFENKKLIIKKPINP"
     gene            9897..10382
                     /locus_tag="PKN53_07210"
     CDS             9897..10382
                     /locus_tag="PKN53_07210"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOD88774.1"
                     /translation="MKTTVKFLAVILFVTFYSANLAFSQEAKKDCKQHQSTCIHKDLS
                     ESQKTSINAIKLASDKKIAVYKADLKIKNAELDKLLIAENPSKKEIDAKIDDLTKLKG
                     DIKKERIDCRLKIREVLTPEQRVNFDLKGKGKKNHKDSQCEHHKEQFHGKHQPCSKSG
                     K"
     gene            10540..11325
                     /locus_tag="PKN53_07215"
     CDS             10540..11325
                     /locus_tag="PKN53_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88775.1"
                     /translation="MKKFLYILILIVFGQSLYAQEVVNSDYTKGIDAFEIDKLNNFYF
                     IKDNQILKTDSEFNKLYDYSDKTFGNIYFIDVSNPFRVLVYYKDFNVIVFLDNKLAPL
                     RSPIYLDDLNYFSADAVCSSSFESFLVFDSQNTRINTISKDLVTTQKGTSLYSIVSEQ
                     KPVKMKESNNYIFISFENGYIHVLDKYANYVDSFFYDGLIDFDCINDDLYLLGNNELI
                     AFDINKEKILYYSFEASNSMGFKIKNDVLYILNKKSLISFKIL"
     gene            11397..12011
                     /locus_tag="PKN53_07220"
     CDS             11397..12011
                     /locus_tag="PKN53_07220"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOD88776.1"
                     /translation="MHIAVAGNIGAGKTTLTKLLAKHYGWSPHYEDVDDNPYLNDFYE
                     DMQRWSFNLQVYFLNSRFNQILEIRKAGKTVIQDRTIYEDAYIFAPNLHSMGLMTTRD
                     FDNYFSLFTLMATLVEAPDLLIYLRASVPKLVQNIQQRGRKYENTIRLDYLKRLNERY
                     EGWIANYNGGKLLIVDVENIDFAQRPEDLREIIDKIDAEIFGLF"
     gene            complement(12208..12807)
                     /locus_tag="PKN53_07225"
     CDS             complement(12208..12807)
                     /locus_tag="PKN53_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88777.1"
                     /translation="MTEKQIEKIKLAIKKNRATLSAEKRKFGGFFDNSGRRYYISDLY
                     MKIADYKGAITYKKWFDKNFPDDIGNSLLSLNWSIAYFELGKIAEAKIYTIDTAFQNI
                     YLHDLLIGKEVKRIGMYEHGHDMLDVAKSIIKDYKKIVTQTYLEWLTIFMDTDEYKEP
                     INKYIELSKLLKDENNKDKWIELLDQIRELEDRNKNRKK"
     gene            complement(13041..14069)
                     /locus_tag="PKN53_07230"
     CDS             complement(13041..14069)
                     /locus_tag="PKN53_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008776413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HOD88778.1"
                     /translation="MDKNKKLQIRNSTAEFLIFTNQSKTSTIEVRYENGTIWLSQKLM
                     AILFDCSVDNISLHLKNIFKSGEIDENSVTEEYSATASDGKNYKTKHYNLDAIISVGY
                     RVNSVRATQFRQWATQVLKEYAIKGYVLDRKRMENGAFLDEDYFEHLLEEIREIRLSE
                     RRFYQKVTDIYATSIDYNKDAIITKEFFAKVQNKLHYAIHQHTAAELIIERADSQKEH
                     MGLTSWKNSPNGKILKSDVTIAKNYLAKDELDALGRIVNAYLDLAENRAKRKIPMTME
                     DWAKRLDLFLQHDDREILQNAGKISYEIAKEHAETEFEKYRIIQDQLFQSDFDKLINN
                     LDNKAINE"
     gene            complement(14281..15345)
                     /locus_tag="PKN53_07235"
     CDS             complement(14281..15345)
                     /locus_tag="PKN53_07235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04133 family radical SAM/SPASM protein"
                     /protein_id="HOD88779.1"
                     /translation="MLNKIPQYFHNRFRANEAKLHELTYLFWECTQRCNLNCLHCGSD
                     CIADSKVKDMPFEDFIEAILPLKDKFKPNSITVVITGGEPLLRKDLPQCGKTLREHGF
                     RWGIVTNGFSYNPDIHARLLAAGMGAITLSLDGLENSHNWLRNNNNSFNKALQALDLI
                     TSSNRLFYDVVTCVNSKNISELPQLLDLLISKNVKAWRLFTISPIGRAVNNDDLQLTS
                     LHLKQLMDFITQSRLDKKIDIKFSCEAYVGKYEKKVRDSYFFCRAGINIASILIDGSI
                     SACPNINRKFVQGNIYNDRFLDVWENRFDIMRNRNWTKTGICLNCKDYKNCNGGAMHL
                     WDEKQDCIMTCINDKLKKNY"
     gene            complement(15335..15721)
                     /locus_tag="PKN53_07240"
     CDS             complement(15335..15721)
                     /locus_tag="PKN53_07240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM-associated putative lipoprotein"
                     /protein_id="HOD88780.1"
                     /translation="MTTKLKEKKHKILRIIFGTLSFSTAVFVFQACYGTPEDFGMDVE
                     IKGYVKSKATNQPIPGIKVSLENQPQYELTDSEGMFKIYASKDSSYKVMFEDIDSTTN
                     GSFLPKDTVVSIIDDTTFLNVALDAK"
     gene            complement(15858..16841)
                     /locus_tag="PKN53_07245"
     CDS             complement(15858..16841)
                     /locus_tag="PKN53_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HOD88781.1"
                     /translation="MTKIIALGNALVDVMTMLPNDSVLEELNLPKGSMQLVDAETSKK
                     IQNFTDNFSKSIASGGSSANTIRSLANLGVSTGFVGKICDDEMGKFFAEDMKRTGVFP
                     HLKKSKTPTGVAQALVSPDSERTFATFLGAASELIPDDISDLTFKGFDILHIEGYLIF
                     NNELIEKALISAKNAGLKVSLDLASYNIVEANLDFLKYLIDKYVDIVFANEEEAKAFT
                     GKEPEQALDMISSMCEIAIVKIGAEGSLVKRTNEREAVGAIKAKPIDTTGAGDNYAAG
                     FLYGLINNWDLRRCAQAGSILAGNVIEVIGATMDEKRWNKIKEEISQLKNN"
BASE COUNT         5521 a         2658 c         2493 g         6293 t
ORIGIN      
        1 tagacgcgtc agcctctttt aacacttcaa ctacgttcag tgcatcgctt ttaactgagc
       61 atagtgtgtt caacttcctc aatactaata ggaattctct tagaacctaa aattctattt
      121 ccagtttcag taattaagat gtcgtcttct agtcttattc caccaaaatt tctatattcg
      181 ttaactttac tgtagtttat aaattcttcg aattttttat ctttttgcca taagtcaatc
      241 aattcaggaa tgaagtagat acctggttcg tttgtaagca catgtccggg ttttaattct
      301 ctaccaaatc ttaggaaagc agttccaaat atttcgcttg gtctaacttt atcatcgtaa
      361 ccaacattgg tttgacctaa atcttccata tcgtgaacat ctaatcccaa agcatgacct
      421 aaaccgtgag gcataaatag ggcatgagca cctaatgcaa cagcttcttg aacattacct
      481 ttcattaatc ctaattcttt cagaccgttt gctaaaattt cgcaggcttg aagatgaata
      541 tccatataag ttttgccggg cttcattaat tttattgaac cgttaattgc atttaaaaca
      601 atttgataaa tttctttttg cttttgtgtg aatttaccac caacaggaac agttctagtg
      661 ttgtctgaag cataaaacat tggtgtttgt gcacctgaat cgtgaagcat taaatcgcct
      721 ttttgcaaaa cattatcacg agagtgattg tgtaatgttt gtccattttt tgttaaaata
      781 actgggaaag aaggaacaga accgtaagac aagctaatgc cttcaacaat tcccgcaatt
      841 tcacgttcta caacacctgg ataagcatgt ttcattgcaa ctgtgtgcat tttataacca
      901 atgtcgcaag ctttttctat ttcttgaatt tctatttctt ctttaatatt tcttaactta
      961 acaattgcct taataaattt tagagaagct tttgctttta gttcttgaaa acttattcct
     1021 aaaagactgt gcaataaaat catgttatca tgcttgtagg caggtaaata actaacttct
     1081 ctaccttttt ttagagcatc tttaatatag tctgaaagct ttgcaaattc gtaagtctca
     1141 aaaactgaac atttgcttgc taattcttta acgcttggtt gaggacccat ccaaattata
     1201 tcatcaatat caacatcgtt tgcgaaaatt atttctttat cattgtcaat atctatgatt
     1261 gcagccatac ccggaaggtt aagtccgaaa aaatacagaa aactgctatc ttgcctaaaa
     1321 tgataggtgt tggcaggata attcataggt gtatcgttat ttcctaaaaa taaatataag
     1381 cctttttgta aatctttttt taggctttct cttctttttt tataaacttc tggtttgaac
     1441 ataatttttt gaattttaaa taatcgttaa atatagacat tttttttaat gaattaataa
     1501 aaaaaattaa ataataataa acacatttat tatttaagca agattagatt aaaatagggg
     1561 aattgattta cttatttttt aataagtttt tttgaatcta aagtttttcc ttctacaatg
     1621 aggcaagcga aatatacacc ggcttttagg tcgtatgtag caatttggat ttctccttgt
     1681 ccagcttgaa tttgaacaga tttaactttg atgcctgtaa aatcaaaaat tgctatttcg
     1741 ccattttgtt cttctggtaa attgtaataa aaattagcat aatcgtttac agggttagga
     1801 actggtgcag aaatttttcc aacttcagga taattatcaa agataaaatt tgaaaaatta
     1861 tgattactat aattataata ggtatttgct tgggaattta gcccaaaaca taagaaaatt
     1921 aaaatcaata tgtaaaaatt agttttcatc taatttataa ctgtacaaat atcgttccta
     1981 aaatcttgta cgtaaaaaaa tcaattaagt tacaaaatta gttaaaagtt aaaagtcgaa
     2041 aaaaaatgta gcgaagctta atttcaattc ttaattctta attattcaat tatcaattgt
     2101 tttttaactt ttaactgaga gaatcatctc tataaactcc ctaacagcac cttcgccacc
     2161 tttttttgtg agatgaataa tatttgggat attttttatt ttttctacag aatttgcagg
     2221 gcaagcagct aaaccaactg ctgacagtaa ttcaaaatca ttaacatcat cgccaatgta
     2281 agcaacttct gaaaggctga tattcaattc tttacatagg ttttttatgg tttctaattt
     2341 gttggtttcg ccatgaaaat gataatctaa ttttaactta tttgctctat ttctatttag
     2401 ttggcggtct tctgttgtga taattccaac tttaacacct ttttctttaa gtagttttat
     2461 acccattcca tcgtgagtgc aaaacttttt taattcatcg ccattttcgg aataatacat
     2521 tcctgcatct gttaaaactc cgtcaacatc agacataaaa agctttatgt cagatatttg
     2581 tgctttcttt acagcatttt gttctgctaa taaaatttca acaattttcc agtctattgg
     2641 ttcgtcaatt tcaatggcgg tgctttcgtg catttcataa attccaattt gaccagaaag
     2701 acggttttta tcttttaaga tattttcaac tttgtttata taaaaagcac cattctccat
     2761 gaacattccg tcaaaatctt gtctgcgagg acgatttcta taatcatagt taatagtgtt
     2821 tccgtctttg tcccagaaaa aacgtttcga cttaacacaa cttaacatgg aatttttgcc
     2881 agagtatgca aattgacgta gagcattgtc aaaatcttca ctttttgtca atggtgaagt
     2941 tgcttgtgca agaacaaaaa tatcttgcga gtttaaatta gctttttcaa gatattctaa
     3001 aacaacagat tcggttgaag ctgtgtcgct tgcattttcc gcactacgca tataaatttc
     3061 taattttgaa aaattaaaag atttagcaat ttcagcgtaa ttagtagaat ctgttgcaag
     3121 tattattttg ttaatctcgc ttgcattttg caaagcattt aaaacccaaa aaaataaagg
     3181 ttttccattt aacaatttga tgtttttatc ttttatagat ttactgcccg aacgaactgg
     3241 cacaaaagca atggtactca tctttttcta tattttaact tgtctctttg aatttgttca
     3301 acttctaaaa cttcggaacg cttgtagttt aaagccttat ggcttgcctt taagtcgcga
     3361 actaattttg ttaatccagg cacttctaaa gatgctgcgt ggtcggttcc tttccaagtt
     3421 ctgtcttttg taaaatgcct ttctatccag cttgcaccta aagtataagc agcaatatca
     3481 atagctattc cgagatgatg tccgctaaaa ccaatatcat aaattctgtt tccgtattct
     3541 gatttaattc tttcaatttc taaaagtgaa acatcttcaa aagctacagg gtagccagaa
     3601 gtacacgaaa acaaaattaa tctttctttg gcggaattat ttttttcaaa aagttttact
     3661 aaggcttctt cttcttcgtg ggttgtcatt ccgagtgatg cttgaatata tccactgtaa
     3721 ttatctctta aatattctag catttcgtaa tgattgttac aagcagaagg aatttttata
     3781 aaatcgggtt ttaagctcgc aatttcttta gcagatgtta agtcccaaac cgaagtggca
     3841 tatcctattg agttggcttc gcaaaacttt tttaattcag catgttgctc aagtgaaaat
     3901 tctaaaaatt ctctatgctc gccgtaagtc ttgccgtaag cgtgcatctc gtttggatgt
     3961 ggtgagttgt attgctcagg agttaataat tctttattat ttctcttttg aaattttgca
     4021 taatcagcac cacttaattt tgctaataaa attaattctt tggcaatttc aaaatcgcct
     4081 ttgtggttgc aaccaatttc tgcaattact ttaggttctt tatatttttt catattcatt
     4141 tgtttttaaa atttaattct tcaaaattta ctctttcaaa agcatcaata aaagaatttt
     4201 cagttaaatt aaaaattttt gcttgcttat aatctgcata ttcttttaaa atgtggtaag
     4261 atttaaaagc ttcgcctaag gtatataaaa tttcgtgtag tttcacataa cgctgtccca
     4321 tataatcaaa ttgtttcgcc ttgatttcct tatcgtaaaa atgttggtta aacagtaaaa
     4381 ctttattttc attatcaact tgtaattctt taagccaaga atgatcaact ccagcaagta
     4441 aaatattttt agcttgtaag tttaatgctg tgaaaatcgc tggaaccatt atgttgtgtg
     4501 gacgaggcat tcctctgagt ttatcaaaat ttctaaattt aaaattttta aatccgtcaa
     4561 tgggagtgag gttgatataa ataatttcga cattagaatt tttctttaaa atttcttgcc
     4621 aacgctgatg ttttcgagct tctgttgtta taaaaaactt taaattccaa tctgtttttt
     4681 caaccaaagt gttgaaaagt ttatttgaag cttcaagaaa atgaggcata acatcatcta
     4741 aatacaagtc atgagctatg gcaatataat attttggttt aagttctgtg taaaattcgc
     4801 ttattgcaaa atgattaaca gcaattaaat tgtaattttt tatcttttct ttatgatttt
     4861 caataaaaga atttaagctt ggaccattac ctaaaattaa aatatctttt tcagattttg
     4921 caaggttttt atattgtttt gttgattttg aaaatagcaa aatctttacg catgatgcac
     4981 aagtttttgc caaaagcctg aaccaagaat ttatttttat taaaaaatct cgcattataa
     5041 ttaatttaac tacaaagtta aattaaattt taaaattcac acactaattg caagtttata
     5101 atttaaactg attttattta cgagtataat ttgaaagttt tacttgattt tgtaacctta
     5161 gataattttt tacgtttaat cacttagaaa attgataaaa tgaaaaagct atttgcagaa
     5221 gatatttttg gtaactgttg taaagatttt ttagctggcg ataaagatgc taaaattgtt
     5281 gtaaattcgg atattgctga aacagattat ttatttgcag agtatttatt tagagaattt
     5341 gatgaaatgc cagaacatga gcaaaaagct ttattgcaag caagaggcga tattttagat
     5401 attggtgctt gtgcaggttc acactccttg tatatgcaaa atatgggtta taatgtaact
     5461 tctttagata tatctcaagg ctgttgcgag gtaatggaaa aaagaggatt gaaaaatgtg
     5521 gtctgttcag atgtgttttc ttattcagac aaaaaatttg ataccatttt attattaatg
     5581 aatggaattg gaatagcagg aaatttacca aatctaccaa aacttttagt tcatctgaaa
     5641 acattgttaa aaccaggtgg caaaattatt tttgattctt cagatttgca atatctatat
     5701 ttagatgacg atggtgctta ctcaattcca ttaacagata gatattatgg agaaatgata
     5761 tataatttat cctataaaga taaaaaaaca ggtaatttta aatggttttt tatagatcca
     5821 tatacaattg aagctattgc aaatgaagtt gatttaaaaa ttcagttttt ggcttgggga
     5881 aatcattatg attattcagc ttgtctgtat taattcagtt gaaagttcat aaagtttgta
     5941 aagttcgtaa agtttgtaaa gttcgtaaag ttcgtaaagt ttgtaaagtt cataaagttt
     6001 gtaaagttca taaagaaaag ctaaatccaa gcgagcaaag taagtttgcg agctcatcga
     6061 aggtaaagtt gctcatcaaa aacgccttga ttttatacaa aaaaacgatg tcttaaaatt
     6121 tcttctaaga catcgttttt agttttatct tttagataag tttatttttt aagttccttt
     6181 ttttgttttc ttaatgctct ttctgttaag tcataaacta taggcgaagc tagacatact
     6241 gaagaaaacg ctccagtagc aataccaatt agtaaagcaa atgtaaatcc acgaataact
     6301 tcaccaccga aaataaatat tgctaaaact actaaaagag ttgtcataag agtatttact
     6361 gtacgaccaa ttgttgagtt gatagcataa tttattgttt cgtctaaagg acgattttta
     6421 tatagttttt gatgttctct aagcctatca aagataacaa cagtgtcgtt tatagaataa
     6481 cctatcaccg ttaataccgc tgcaataaag gtctggtcaa cctccatatt aaacggcagt
     6541 atattgtcga ataaagaaaa tactcccaaa attaataaag cattatacgc taaagcaata
     6601 gttgcaccag ttgaatattg ccattttgta aatctaacta aaatatagat aaacattaga
     6661 gctaaaccta taataaccac taagatagat tttttaacaa tatcagaggc tactgaaggt
     6721 ccaacttttt gtgctctaac tttataatca tcattaaact tttcgtagct aacatcatcg
     6781 ccgataagag gttttaagcc tacatacaat aatgagtcaa caagttggtc tgtatctttt
     6841 tctttaattt tatatttagt agtaattttt acttgatttt tagtaccata agttttgaca
     6901 ataggtaatt cttcataaac agtttctaat gctttagcaa cttcttctgg ctcaacagct
     6961 tgttcgaatt ttacaatata atcacgacca ccagtaaagt caacaccttg gtttaaacct
     7021 ttagtgaaaa gaagaataat actaattact gctaacgaac cggcaatagt atagaaaatc
     7081 ttacggtttt ttataaaacc aatatttagg tcagtaaata gattgtttgt aaatttatta
     7141 ccaaaattga ttttcttttc tttctttaat aatgatgcga atatcaatct agtaatgaat
     7201 agtgatgaga atagagaagt taaaatacct ataattaatg tagttgcgaa acctttaaca
     7261 ggacctacac caaacattgc aagcacaata ccaactagca aagttgtgat gttaccgtca
     7321 agaatagcag gataagacct tttgtatcca tcaactaaag ctgattttat ttgttttccg
     7381 gctctaattt cctcgcgaac ccgctcataa ataataacgt tggcatcgac cgccatacct
     7441 agagttaata caatacctgc tatacctggt aaagtaagaa cagctcctat agaagctaaa
     7501 actccaaaaa tgaagaatac gttagcaata agtgcgatgt ttgcaaccaa tccagctctg
     7561 ttgtagtaga aaatcatgta tattagcacg attataaatg ctataataaa tgacgcaaaa
     7621 ccagaatcta ttgctttttg tcctaatgaa ggtccaacca cttcgctaga aataatggaa
     7681 accgaaacca ccatttttcc tgatttcaac acatttgcca agtcggtagc ttcgtctaga
     7741 gtaaagtttc cagaaatgct tgagtgtcca cctttaattt cgtcgtttac gtttggataa
     7801 gaataaactt gtccgtcaag aacaattgca atactttttc caatgttttc ttttgtaatt
     7861 cttgcccatt ctgtagcacc agccgagttc atagacatcg aaacgtgagc ttgaccagtt
     7921 ctttcatcaa attcttgtct agcatctgta acaacatcgc cacttaaagc agctccgcca
     7981 gttgctttac ttctttgtag aacaacaact tcgtaataat ctatatttcc ttctactggt
     8041 ttatatccgc aagcaaaaac aacatcattg ccaaaaaagc cagctaaata atctgcattc
     8101 aaatcattta tcaatgcatc tttgtcagct tttttaacat aacccaaaac tggtccatat
     8161 acgcctctgt cgccaatatt aaataccata cgtttggtaa tacttctagt ttgagtttgc
     8221 tcacttttat ttgtttctgc atcaacaata ttagtagtgg tgtcaatatt agtagtatca
     8281 gcaacaatat tggtagtgtc gatagcaata gcttcttctt cgttttgagt taagaaatca
     8341 tcaactttgg ctaagttgtt tcttatttca gtagcatctt tagtttccca aaattcaaga
     8401 acagcagctt gtttcaacaa atcttcaaca cgttcaggct ctttaatacc tggcaattct
     8461 atatggaaaa cacctctgtt agcaatgtct ttttgaatat ttggcgaaac aacaccaaaa
     8521 aggtcgatac gttttcttaa aactacaaaa gcattatcga tagcatcttc gtattgtgca
     8581 ttaatataat caattacttc ttggtcagtt gatttagcgt taattttatc gctattgcta
     8641 attttgctaa ataaagcagc taagctactt gattttggag tttttacttt tgaataagca
     8701 tctccaaaaa tggtgataaa atcggtttcg tcatcgccgt caattatttt attagtttct
     8761 tcaagtgctg caagcaaggt tgggtcgtta ggattattag ctaaagataa aattaaatct
     8821 tctgccgaaa ttcttaaact aacgttcata cctcctttaa ggtcaagacc aaggttaagt
     8881 tccctctttt tgcaatccat ataagtatgt tttgaaaata gggttaaagc aggtaaattc
     8941 tcaactgaat caagataata ttttactaaa gagtcagtaa tttctaactg tctagcttcg
     9001 tcaaccttta aattcattgc agtaaattga gattctgcat atttttctgc tttattttct
     9061 actcttttaa cagcaaatgt gaatgttaaa tagtatagac aaatcaatgt caatagcact
     9121 gttactaata caatcgctcc tttgttacgc atatcagtta aatttattct ttaattatta
     9181 ctatgtttat tttaaagttt gcaaatatag accttttttt taaattttaa tatgttttta
     9241 tgttttattt tattgctagt ttaagttagc taaagcaaga acagttattt gtaagtttta
     9301 aaaatatatt tttataaaaa aatatatcct tgctttttgt aacttgcagt tatttaggga
     9361 tttataggtt ttttgataat taattttttg ttttcaaaat ctaaaacagc attattctca
     9421 ataagaaaat cacaacctaa tatgcctgct acatgaggta aattaaggtt tttatacaaa
     9481 tagtttatat gttgcatcga agtaaatacg gcttcaaaat tttttatttt gaaccacgaa
     9541 atcgaaaatg aatcgatgtt tccgaaatat aattcttcaa taacagagtt gaagccagaa
     9601 ctaggattgt cggtttctag gttttcaaaa tcaatattag aaaaaatagg attttctaag
     9661 tcaaataccg tactcgaagc tccagtgtca acaatcatat tgactttgtt tttattgact
     9721 tttgctttga taagtacatg atttccaccg ccttcaatgt taagtatgtg aagtttactt
     9781 tggtatgttt taaatttttt actcataaaa tttagcaata taatattttt ttgttttgca
     9841 ttaaaccttt tctaatttta attgtctata attctgtaat caaaaatata aaaattatga
     9901 aaactaccgt aaaattttta gcagtaattt tgtttgtaac tttttattct gcaaatttag
     9961 ctttttctca agaagcaaaa aaagattgca aacaacatca aagcacttgt attcacaagg
    10021 atttaagtga aagtcaaaaa actagtatta atgcaatcaa acttgcgagc gataaaaaaa
    10081 ttgcagtata taaagcagat ttaaaaatta aaaatgctga attagacaag cttttaattg
    10141 cagaaaatcc atcgaaaaag gaaattgatg caaaaattga tgatcttaca aaattgaaag
    10201 gagatattaa aaaagaaaga attgattgtc gtttaaaaat tcgcgaagtc ttgactccag
    10261 aacaaagagt taattttgac ttaaaaggta aaggtaagaa aaatcacaaa gattctcaat
    10321 gtgagcatca taaagaacaa tttcatggca aacaccagcc ttgttcaaaa agtgggaaat
    10381 aaatatattt aattaattta atgtgaactg cttgataaat tttctgtcaa gcggtttttt
    10441 tattgcgatg cggttcataa aatttgacta aaaaaacaca acttaaaact ttttttgtat
    10501 ctttaccgtc ttggaataaa ttttgtgaag tattattcta tgaaaaagtt tttatacata
    10561 ttaatattaa ttgtttttgg tcaaagttta tatgctcaag aagttgttaa ttcagattac
    10621 acaaagggta tagatgcttt tgaaattgat aaactcaaca atttttattt tattaaggat
    10681 aatcaaattt taaaaaccga ttctgagttt aataaattat acgattattc cgacaagact
    10741 tttgggaata tttattttat tgatgttagc aatccattta gagttttagt ttattataaa
    10801 gattttaatg ttattgtgtt cttagacaac aagttagctc cgttaaggtc gccaatttat
    10861 ttagacgatt tgaattattt ttcagcagat gcagtatgtt cttcttcttt tgaaagtttt
    10921 ttagtttttg attcgcaaaa tacaagaata aatacaattt ctaaagacct tgtaacaact
    10981 caaaaaggga caagtcttta tagtattgtt tcggaacaaa aacctgtaaa aatgaaagaa
    11041 agcaataatt atatttttat aagttttgaa aatggatata ttcatgtttt agacaaatat
    11101 gcaaattatg ttgattcatt tttttatgat ggattaatag attttgattg tataaatgat
    11161 gacttgtatt tgttgggaaa taatgagctt atagcttttg atattaacaa agaaaaaata
    11221 ttatattata gctttgaagc tagtaatagc atgggtttta agattaaaaa tgatgtttta
    11281 tacattttaa ataaaaaatc gttgataagt tttaaaattc tttagtaatt tttttttgct
    11341 taattattaa tattgtatat atttattttt tttaataatt acaaaatttt aagaatatgc
    11401 acatagcagt agcaggaaat attggcgctg gaaaaaccac attaaccaag cttttagcca
    11461 aacattatgg ttggtctccg cattacgaag atgttgacga caatccttat ttgaatgatt
    11521 tttatgaaga tatgcagagg tggtctttta atcttcaagt ttattttttg aatagtagat
    11581 ttaatcaaat tcttgaaatt agaaaagcag ggaagactgt aattcaagat agaaccattt
    11641 atgaagatgc ttatattttt gcacctaact tgcattctat gggtttgatg acaacccgcg
    11701 attttgataa ttattttagt cttttcacgc taatggcaac tttggtagaa gctcctgacc
    11761 ttttaatata tttgagagct tcggttccaa aacttgtgca aaatattcaa caacgtggac
    11821 gaaaatacga aaatactatc cgcttagact atctaaaacg cttgaatgaa agatatgaag
    11881 gctggatagc aaattataat ggtggcaaac tattaattgt agatgttgaa aacatagatt
    11941 ttgcacaacg tccagaagat ttgcgagaaa ttattgataa gattgatgct gaaatatttg
    12001 ggttgtttta atggcttatt tcgacttcgc ttatttcggc tccgctcaat aagcgtataa
    12061 ttgctgacag agttaaagtt tgaaccgagc ttgagagttc gctgaaagaa aaagttccta
    12121 aagttgaatt tttataaaaa tgtgtcaaat ttttttggga caagaaaaac ttgtgagatt
    12181 agtgagggga agatgagtgg gatggaatta cttttttctg tttttatttc tatcttctaa
    12241 ttctctaatc tggtcaagta attcgatcca tttatctttg ttgttttcat cttttagaag
    12301 tttgcttagt tctatgtatt tattaattgg ttctttatat tcgtctgtat ccatgaaaat
    12361 tgtaagccat tctaaatatg tttgtgttac aattttttta taatccttaa taattgattt
    12421 tgcaacatca agcatgtcat gtccatgttc gtacatgcca attcgtttta cttctttacc
    12481 aattagtaaa tcgtgtagat aaatattctg gaaggcagta tcaatagtgt aaatttttgc
    12541 ttctgctatt tttccaagtt caaaataagc aattgaccaa tttaaagata ataatgagtt
    12601 tccaatatca tctggaaagt ttttgtcaaa ccatttttta tatgtaattg ctcctttata
    12661 atctgcaatt ttcatgtaca aatcagaaat ataataacgt cttccagaat tatcaaaaaa
    12721 tccaccaaat tttctttttt cagcagacaa tgtagctcgg tttttcttaa tcgctagctt
    12781 tattttttcg atctgttttt ctgtcataat tttcaatgtg tttgtggtct agcactattg
    12841 aacaaatatc tatttttcaa aacaaatata agccattcct ataaattaaa caaataaatt
    12901 ttgtgtgtta tttttttgtt gggtgcaaca ctcggattgc aaccgaagtc cgcagaacga
    12961 gttcagcaaa gttgaatctg agcgagcagg ggagaagttt ttgaaatttc aatcattctt
    13021 acactttgtt ctggtgtttt ttattcattt atcgctttat tgtccaaatt attaattaat
    13081 ttatcgaaat cagattgaaa taattggtct tgtataattc tgtatttttc aaattcagtt
    13141 tctgcatgtt cttttgctat ttcataactt attttgccgg cattttgcaa aatttctcta
    13201 tcgtcatgtt gaagaaataa atctaaacgt tttgcccaat cttccattgt cattggaatt
    13261 ttgcgtttgg ctctattttc tgctaaatca agataagcat tcacaatcct tcctaatgcg
    13321 tctaattcat cttttgcaag ataatttttt gcaatcgtaa catctgattt taaaatttta
    13381 ccgtttggac tgtttttcca tgaagttaat cccatatgtt ctttttgact atctgcacgt
    13441 tcaataatta gctctgccgc agtgtgttga tgaattgcat agtggagttt gttttgaact
    13501 tttgcaaaga attcttttgt aattatagcg tctttattgt aatcaatact tgttgcataa
    13561 atatcagtaa ctttttggta aaaccgcctt tcactcaatc gaatttcacg aatttcttca
    13621 agtaaatgct caaaataatc ttcatctaaa aaagcaccat tttccattct ttttcggtca
    13681 agaacatatc cttttattgc atattctttc aatacctgag ttgcccattg cctaaattgt
    13741 gttgcacgaa ctgaatttac cctatatcct acagatatta ttgcgtctaa attgtagtgc
    13801 tttgttttat aatttttacc gtctgaagca gttgccgagt attcctcggt aactgaattt
    13861 tcgtcaattt ctcctgattt aaaaatgttt ttcaggtgta atgaaatgtt gtccacgctg
    13921 caatcaaaca aaatagccat aagtttttgt gaaagccaaa tagttccatt ttcgtagcga
    13981 acttcaattg ttgatgtttt actttgattg gtaaaaatta aaaattcggc tgtgctattt
    14041 cgtatttgta gttttttatt tttatccatt tttcaatatt ttgtacggct ataaaattgc
    14101 tgtttttcag atagactctg atattcgttc aattgagtat attatttgtt ttctaaaaca
    14161 aatatacaac attcatataa atttagcaaa taatattgta tgttatttat taatttaatg
    14221 tcaagcggag gagtggaaag gggagttcgg ttgatttaag tccgagagtg tgggttggca
    14281 ctagtaattt tttttcaatt tatcatttat acaagtcatt atacaatcct gtttctcgtc
    14341 ccacaaatgc attgcacctc cgttacagtt tttataatct ttgcaattta aacaaattcc
    14401 cgtttttgtc caattacgat ttcgcataat atcaaatcga ttttcccaaa catcaaggaa
    14461 tctgtcattg taaatattgc cttgaacaaa tttgcgattg atatttggac aagcagatat
    14521 tgaaccatca attaaaattg atgcaatgtt aattcctgcc ctacaaaaga aatatgaatc
    14581 tcgtactttt ttttcatatt ttccaacata tgcttcacaa ctaaacttta tatcaatttt
    14641 tttatcaaga cgagattgtg taataaaatc catcagttgt tttaaatgca gactagttaa
    14701 ttgcaaatca tcgttattta cagctctacc aataggagaa attgtaaata atcgccatgc
    14761 cttaacattt ttagaaatta gtaaatccaa taattggggc aattctgaaa tgtttttaga
    14821 atttacacag gttacaacat cgtaaaaaag tctattggaa gaagttatta aatccaatgc
    14881 ctgcaaagcc ttattaaaac tattgttgtt atttctaagc caattatgtg aattttctaa
    14941 tccatcaagg cttaaagtta ttgctcccat tcctgctgct agtaatcttg catgaatatc
    15001 tggattgtat gaaaatccat ttgtaacaat tccccaacga aagccgtgtt ctcgtaaagt
    15061 cttaccacat tgaggcaaat ctttgcgtaa caatggctct cctcctgtaa taacaacagt
    15121 tattgaattt ggtttaaact tatcttttaa tggcaaaatt gcttcgataa aatcctcaaa
    15181 tggcatatct tttacctttg aatctgcaat acagtcagaa ccgcaatgca aacaatttaa
    15241 attgcagcgt tgtgtgcatt cccaaaataa ataggttaat tcatgcaatt tagcctcatt
    15301 tgctcgaaat ctgttatgaa agtattgagg gatcttattt agcatctaaa gctacattta
    15361 gaaatgttgt atcgtcaatt atgcttacaa ctgtatcttt gggtaaaaag ctaccatttg
    15421 tagttgaatc aatatcctca aacataactt tgtatgacga atcttttgaa gcataaatct
    15481 taaacattcc ttcactatct gttagttcat attgtggttg attctcaaga gagaccttaa
    15541 tgccaggaat tggttgattt gtagcctttg attttacata tccttttatt tcaacatcca
    15601 ttccaaaatc ctcaggtgtc ccataacaag cctgaaatac aaatactgct gttgagaagc
    15661 ttaatgttcc aaaaattatt ctcaaaattt tgtgtttttt ttctttaagt tttgtggtca
    15721 taattgtcta atttaaagaa agttgtaggt ttagtttcta attgcaacta acatttattc
    15781 tccaaaacaa atataaaaaa ttcatctaaa ctaaaaaatt cttaattctc aattcttaat
    15841 tatttaattc ttaattttta attatttttt agttgagaaa tttcctcttt aattttgttc
    15901 catctttttt cgtccattgt agcaccaatg acttcgataa cattgcctgc taatattgag
    15961 ccagcttgcg cacaacgtct taaatcccaa ttattgatta agccatataa aaatcctgcg
    16021 gcataattat cgcctgcacc agttgtgtct attggttttg cttttattgc tcctacagct
    16081 tcgcgttcgt tagtacgttt tacaagggag ccttcggcac caatttttac aattgcaatt
    16141 tcgcacattg aagaaatcat atctaaagct tgttctggct ctttgcctgt aaaggcttta
    16201 gcttcttctt cgtttgcaaa aactatatca acatatttat caattaaata tttcaaaaaa
    16261 tctaagtttg cttcaacaat attgtaagaa gctaagtcca gcgaaacttt taaacctgca
    16321 tttttcgcac ttattaatgc tttttcaatt agctcattat tgaaaattaa atatccttca
    16381 atgtgtaaaa tgtcaaaacc tttaaaagtt aaatcactaa tatcatcggg aattaactct
    16441 gaagcagcac caagaaaagt tgcaaaagtt ctttcagaat caggactaac taatgcttga
    16501 gcaactcctg taggtgtttt cgattttttt aaatgtggaa aaactccagt tcttttcatg
    16561 tcttcagcaa agaatttacc catttcgtca tcgcaaattt taccaacaaa acctgttgac
    16621 actcctaaat ttgctaagct acgaatagta ttggcagaag aaccaccact tgctatactt
    16681 tttgaaaaat tgtctgtaaa attctgaatt tttttgcttg tttcagcatc aactaactgc
    16741 atactgcctt ttggaagatt taattcttct aaaactgaat cgtttggaag cattgtcatt
    16801 acatcaacta aagcgtttcc tagtgctatt atttttgtca tataaaaaaa aattaaaaat
    16861 tattttgcaa atataaaaaa caatattatt tttgcaatgc ttttttcctc tttagctcag
    16921 ttggttagag cacctgactg ttaatcaggg ggtcctaggt tcaag
//
LOCUS       DAOPNX010000056        16944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_4039_length_16944_cov_6.385636, whole genome shotgun sequence.
ACCESSION   DAOPNX010000056 DAOPNX010000000
VERSION     DAOPNX010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16944
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4039_length_16944_cov_6.385636"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..101)
                     /locus_tag="PKN53_07250"
     CDS             complement(<1..101)
                     /locus_tag="PKN53_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOD88782.1"
                     /translation="MSRSLKKGPFVDFKLERKVIAMDASGKKNAIKT"
     gene            complement(113..937)
                     /gene="rplB"
                     /locus_tag="PKN53_07255"
     CDS             complement(113..937)
                     /gene="rplB"
                     /locus_tag="PKN53_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOD88783.1"
                     /translation="MALRKINPTTPGQRFKQISAFDDITCTTPEKSLLVPMKKSGGRN
                     NQGKMTVRNIGGGHKRQYRLIDFKRDRDGVKARVKTIEYDPNRSARIALVVYEDGEKR
                     YIVAPNGLQIGQIIESGKGAAPEVGNALFLSDIPLGTVVHNIEMRPGQGAVIARSAGT
                     FAQLVSREGKYAILKMPSGETRKILVTCKATVGSVSNSDHALENSGKAGRSRWLGRRP
                     RVRGVAMNPVDHPMGGGEGRASGGQPRSRTGLLAKGFKTRAKKKESNKYIIERRKK"
     gene            complement(947..1237)
                     /gene="rplW"
                     /locus_tag="PKN53_07260"
     CDS             complement(947..1237)
                     /gene="rplW"
                     /locus_tag="PKN53_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOD88784.1"
                     /translation="MNILIRPIVTEKMTKQGETLNRYGFIVDKRAEKLQIKKAVEQMY
                     DVKVEAVNTMRYAGKLKVRYTKRGIQRGKTNAFKKAIITLAQGEVIDFYSNI"
     gene            complement(1250..1876)
                     /gene="rplD"
                     /locus_tag="PKN53_07265"
     CDS             complement(1250..1876)
                     /gene="rplD"
                     /locus_tag="PKN53_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOD88785.1"
                     /translation="MKTSVYNKLGEDTGRKIELNDLIFAIEPNDHAIYLDCKQYMANK
                     RQGTHATLHRGLVSGSTRKIKRQKGTGTARAGSIKNPLFRGGGTTFGPEPRLYHIKLN
                     KKLKKLARKSALSYRAKENKIFVVDNLLFDEIKTKNIINLKNNFKINDKKITLVVAES
                     NKSVYLSSRNLKDVNVVTASDLSTYDIMWASVLVMEEKSIDVFENNLA"
     gene            complement(1876..2517)
                     /gene="rplC"
                     /locus_tag="PKN53_07270"
     CDS             complement(1876..2517)
                     /gene="rplC"
                     /locus_tag="PKN53_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOD88786.1"
                     /translation="MQGIIGKKVGMTSIYSVDGKNIPCTVIEAGPCVVTQLRTLERDG
                     YEAVQLAFDEKKEKHTTKAMRGHFKKAGTSPKRKVVEFKDFGQIKKGETYSDLELGDL
                     VTVDIFIEGEYCDVAGISKGKGFQGVVKRHGYSGVGEQSHGQKDRQRAPGSIGAASTP
                     ARVFKGKKMAGRMGGQKVKVESLRVLKVIPESNLLLVKGSVPGSKGSYLIIER"
     gene            complement(2538..2846)
                     /gene="rpsJ"
                     /locus_tag="PKN53_07275"
     CDS             complement(2538..2846)
                     /gene="rpsJ"
                     /locus_tag="PKN53_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOD88787.1"
                     /translation="MNQKIRIKLKSYDYNLVDKSAEKILKTVKTTGAVVRGPIPLPTH
                     KRIFTVNRSTFVNKKSREQFELSSYKRLLDIYSTTSKTIDALMKLELPSGVEVEIKAS
                     "
     gene            complement(2861..4966)
                     /gene="fusA"
                     /locus_tag="PKN53_07280"
     CDS             complement(2861..4966)
                     /gene="fusA"
                     /locus_tag="PKN53_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOD88788.1"
                     /translation="MNQKLINTRNIGIMAHIDAGKTTTVERILYYTGINYKIGEVHEG
                     AATMDWMEQEQERGITITSAATTTFWTTNEQNYKINIIDTPGHVDFTVEVERSLRVLD
                     GAVAVFCAVGGVEPQSETVWYQANKYNVPRIAFVNKMDRTGADFIKVVQQIEEKLNAK
                     PLIIQLPIGDGDEFVGVIDLIEQKAYVWDDDDSLGAKYRVEDIPEEMKDEVFKYREKL
                     IESVIEVDDIMLERFFEDRSKISVQEFKNLLRKLVINKNYIPILCGSAFKNKGIQLLL
                     NAICDFLPSPLDIDSIKGHHPKTDDLIERAADASAPLSAMCFKIVSNSFVGKLAYLRV
                     YSGKIKSGEMVYNVRTGKKDRVSRIFQMHSNKQTQIDVVEAGDICAVVGLKDTITGDT
                     LCDESKPIILDKMQFPEPVISIAVEALSQADIDKLTQALSKMAEEDPTFNLRHDENTG
                     QTLVSGMGELHLEIIIDRLVREYGLKLNQGKPQVNYKEEFIDTINNYNYIFKKQTGGK
                     GLFAEITVNIGPVNDRDFVGLQFVDNIKGGKLPKEYINAVEKGFKQAMSNGPIGGYPM
                     HNMRVELVDGSYHSVDSDALAFQIAAGLCFSEVSKKLKSTILEPIMKLEIVTPDEYLG
                     DINADLNRRRATILGMEMSGMHRVLKARVPMAEQFGYITALRTLSSGRAVFSMEFSHY
                     ERVPATILEELINNRKFIF"
     gene            complement(4979..5455)
                     /gene="rpsG"
                     /locus_tag="PKN53_07285"
     CDS             complement(4979..5455)
                     /gene="rpsG"
                     /locus_tag="PKN53_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOD88789.1"
                     /translation="MRKSKPKKRIILPDPKFNDSRVTRFVNDMMWDGKKSTSFQIFYD
                     SLEIIEKKMKDSEDAPIDIWRKALDNVTPAVEVKSRRIGGATFQVPTEIRADRKISVS
                     HKWLIAAARKRSGQSMSNKLAAEIMAAYNNEGAAVKRKDETHRMAEANKAFAHFKF"
     gene            complement(5481..5888)
                     /gene="rpsL"
                     /locus_tag="PKN53_07290"
     CDS             complement(5481..5888)
                     /gene="rpsL"
                     /locus_tag="PKN53_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOD88790.1"
                     /translation="MPTIQQLVRKGRRKMTYTSKSPALDSCPQRRGVCTRVYTTTPKK
                     PNSAMRKVARVRLTNGKEVNAYIPGEGHNLQEHSIVMVRGGRVKDLPGVRYHIVRGAL
                     DTAGVDGRKQGRSKYGAKRPKKGAAAAATKGKK"
     gene            6202..7293
                     /locus_tag="PKN53_07295"
     CDS             6202..7293
                     /locus_tag="PKN53_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88791.1"
                     /translation="MKNYFYILLSVIAVVGIFFTACDPDDDNKDKNVIEINEDITSAT
                     VWSGDKTYIIKKFDFYVTDVLTIEAGAIIKFSSQAKSLTLSNNGQILANGTASQPIVF
                     TSIKDDSKGGDTNKDGDDSSPAIGDWAKIDLNGTKTSVFNYCEFYYGGFGTGASPTLD
                     LSAESAAKIKNCTFAHNGGGINGKYYIGTIHADNASSETVIQNNLFYDNILPLTITGD
                     ISLDNSNTFSKDGTANKYNGIFVSRNVTKNTTWLEDEIAYVITSDEIQIQLNASLTLG
                     DNVVLKFSEASRLDIVQTESSLGNYNGNGVYFTSLKDDELKGDTNGDENASTPQTADW
                     IGIHINGWKSTGYANWSNIRYNDPNPAVK"
     gene            complement(7358..7714)
                     /locus_tag="PKN53_07300"
     CDS             complement(7358..7714)
                     /locus_tag="PKN53_07300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOD88792.1"
                     /translation="MSKTINLGNKGEDLAAEMLAEKGYVIISANWRDKHKEIDIIAMD
                     DDTLVFVEVKTRSENFWGNPEEFVSRKKQKLLINAAEAYIAETGFTGNSRFDVVSVLY
                     LKSGMEIEHIIEAFYP"
     gene            complement(7778..8506)
                     /locus_tag="PKN53_07305"
     CDS             complement(7778..8506)
                     /locus_tag="PKN53_07305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD88793.1"
                     /translation="MKRIILLVFSLFLVVGILSSQTIDIIDSTGVVTNTTSKVYNVAP
                     GTTYEYKVDLKNNTSNQVEVKMYAYGEGGTINDWVVSFCSPIFAAGGGQCSNPGFPSA
                     KITLAANETTSTPGSINFTQGSTSGEAIIKIEVADVNGGFADSFIIIFSTGFPSSISS
                     ELVKEFSIFPNPASDNFKIKHNYGPNAVVEVYNMLGKLVTKVKSDFVNGVNIDASKWD
                     NGYYFCRLYNDGKIQKTIKLVVAH"
     gene            8842..10521
                     /locus_tag="PKN53_07310"
     CDS             8842..10521
                     /locus_tag="PKN53_07310"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOD88794.1"
                     /translation="MKRSILLFATLLFVFASFAQNKDANTSPICQEPTNDRGTQALPW
                     AEMQMWTATDIKGNTHNLADYLATGKTVFVDFSATWCYPCWSFHQSGVLENLHNQFGP
                     GPDGTNEIVVLWFEVDGATIEQIHGTGSNTQGDWTENDTWPVPIIQASSQLKSYFSEL
                     YPGAVPTMFMICPTGYYRTVDASTSVSYYMSQINSCPEAGQVPLADIDGSAIAFIDTP
                     STFQADAVSIDPITGYEWTFENGDPATSDQPNVGVTWNTPGQNIISLKIHNENGPSSP
                     KYKTVKVIDPATIDNNYLTFEEIPVGNTYPKNLYPYSWTSVNRDNGTVYGSLTNYGVS
                     GNNMSFVVYSKSATSPSHLNVAPKSGDKCALAMTNASGGTNNDWLISPKIQLGTNSSF
                     EAYVKSLTANYGLEKYKIMISTTNTEEASFTMLGSVSTAPVNWTKITRDLSAYDGQEV
                     YIALVYVGYDNFIFFADDIKITTTPTAIETNNLELEARIFPNPANDVININYVEGAKI
                     QIFNNIGQEVYSLANANEYNQIDMSAHQAGSYVVRVTKGDKTNNYKMILVK"
     gene            10609..11112
                     /locus_tag="PKN53_07315"
     CDS             10609..11112
                     /locus_tag="PKN53_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006256216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOD88795.1"
                     /translation="MKRFILIISLSLFISSLFAQGVPSVMIKDIKGKAVNTKAITQND
                     GKPVLICFWATWCKPCIKEFNAINEEYDDWVDETGVKVIAISIDDARSSNSVAPFVNA
                     RGWEFPVYLDPNGDFKRAMNVGDIPHTFLLDKNGKVVWQHTTYLEGDEAKTFEVIKKV
                     AKGQAIK"
     gene            11403..13061
                     /locus_tag="PKN53_07320"
     CDS             11403..13061
                     /locus_tag="PKN53_07320"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HOD88796.1"
                     /translation="MKKIILFILTVLLLNHAYSQNETQANYGSISGNFQSEAQYYITD
                     SLIGATAPIEKTLANSWANITYTLKNFSAGVRYEGYLNSILGYPNSGGRNDGIGFPHR
                     WANFVVDKFEFTVGNFYEQFGNGLVLRSYEEKRLGVDNSLDGFRVKYNPAQGVYLKTL
                     IAKQRFYWEHGPGIVRGFDGEIQFNDLIEKFAESDLRISLGGSFVSKFQKEESPIYNL
                     PKNVAASAGRIDIRYNGLAINSEYAYKINDPGADNNYIFKNGQAFMVNASYSQKGLGI
                     VLSTKWVDNMAFRSDRNASLTDLNINLLPELTRNHVYMLPAFYPYASQPKGEWGAKAE
                     IMYKIPKGNVLGGKYGTTILVNYSRVHNIDKQAINDTLPLDAPGTLGYNTSFFSIGKE
                     LYFQDAHIEINKRWTKKLYTNLSYVNLLYNYNILRGTTGHDNVNAHIAIADITYKFKP
                     QLALKSEFQHMYTKQDEGSWAMALFELTTSGWFFTVLDNWNYGNPDKNHRPHYFNVGF
                     GHISGATRIQLTYGKTRAGVMCIGGVCRNVPASNGISLSISSTF"
     gene            13073..13930
                     /locus_tag="PKN53_07325"
     CDS             13073..13930
                     /locus_tag="PKN53_07325"
                     /inference="COORDINATES: protein motif:HMM:NF033782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28 family outer membrane lipoprotein"
                     /protein_id="HOD88797.1"
                     /translation="MKSKYFNIIILSAFVALFFSCDEIEGPYIKDEATIWNGKKTLIL
                     DFTGHCCGNCPRAHKTIDELVEKFGDAIVPVAVHSTYFAMPETTDTSKPFHYDFRTSF
                     GDFWGGRDYAFGHLGEIYLPTGLVNNLNPDQVTNTNAWAGLIASVLANYPEYNIEIYP
                     SYSNSDSLAKADIKIITNIKNHRELSLLVFIVEDGIIQWQKDYSATPNNIKEYEHNHV
                     LRASFNGNFGDKIKDNNAETIIGDEINKSYSLKINKDWNINNCSIVAFVYDTETKEVL
                     QAETKIVNE"
     gene            13923..14570
                     /locus_tag="PKN53_07330"
     CDS             13923..14570
                     /locus_tag="PKN53_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOD88798.1"
                     /translation="MNKFLILIFLTLFSISLNAQSFYGGIIVGTTLSQVDGDDYGGFH
                     KISPLGGVYVRNTYNSNWGTSLGIEYKQKGSKDVSKDEYGYVTNYYAMRLDYIEVPFL
                     VNYKLNKFKIPKLVDYSFKNDFWIDFGVSGAYLIKGTDDYGHGPTLPVREFRKYEVAC
                     HFGINYYFNSHWIFHTRFSYTFPLLPIRKHPGGQVYWLNRGQYNHNLSFAIKYEF"
     gene            14570..15157
                     /locus_tag="PKN53_07335"
     CDS             14570..15157
                     /locus_tag="PKN53_07335"
                     /inference="COORDINATES: protein motif:HMM:NF004685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HOD88799.1"
                     /translation="MDSKKKNILVAITGGSGAIYAKQLLEKLNDLKEKESLEISVIYS
                     KTGKEVAIHEIGYDFVKELNLKTYEDNNFYAPFASGSSPADILVIAPCSMGTLGRIAN
                     GFSDSLITRAADVVLKERKKLILLTRETPLNLIHIKNMELISLSGGIIFPASPSFYNK
                     PTNIEDLVNTVIFRILDLMQIEITYDRWGKSVKSL"
     gene            complement(15349..16554)
                     /locus_tag="PKN53_07340"
     CDS             complement(15349..16554)
                     /locus_tag="PKN53_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88800.1"
                     /translation="MAILFSLKFIGGLSLYLIYTYYYEDKKTSDVHKYFRAGCVLHSA
                     TEESYADYFRLLTGIQGDRENLEKYYDQTDHWTRQNSYGLFNDSRTIMRFNALLCLVS
                     RGNIFIHIITMAFMSFLGAFALFKAIDKLTGLNKYLNVVACFLIPSCLFWTSGLLKEG
                     LVMFALGFAFYYMVKIYFKFKILDFILFLFFLFLVGISKIHVLPVFLPMVVFFFIAKR
                     FKTRGRVLILISISLLGFMAIVLSDKIIGYDIMAAIAGKQNDFINYTELEEGQTSTFK
                     LTRLEPNVKSFLKIIPEGLLNSFFRPFPNEINSPVILLSFLEVLFFSLVLIFTIIFFK
                     KPDGDRMLFVLFSLGFVLYLFLLVGAYTPNSGAIVRYRSIGLPFLYAMLFCLWDLEKL
                     KKLLPLKIR"
     gene            complement(16709..>16944)
                     /locus_tag="PKN53_07345"
     CDS             complement(16709..>16944)
                     /locus_tag="PKN53_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOD88801.1"
                     /translation="FGTPYGSLPELVKPEFGFLSSKKEDLSQAIKNYADYNSNHISEY
                     ARDCFNAKVMATEYLKKYEKVISREKLNPENPN"
BASE COUNT         5729 a         2938 c         2491 g         5786 t
ORIGIN      
        1 catgttttaa tggcattttt ctttccgctt gcatccattg caattacttt tctctctagt
       61 ttaaagtcaa caaatggacc ttttttaagt gatctactca taattcacca aattattttt
      121 tcctacgttc aattatatac ttattggatt ctttcttttt agctctagtt ttgaaacctt
      181 ttgctaataa tcctgtgcga gagcgaggtt gacctcctga agctcttcct tcaccaccac
      241 ccattgggtg atcaactggg ttcattgcaa ctcctctaac tcttggacga cgaccaagcc
      301 atcttgaacg acctgcttta cctgaatttt ctaaagcgtg gtctgagtta gacactgaac
      361 caacagtagc tttacaagta accaaaattt tcctagtttc tcccgaaggc atttttaaaa
      421 ttgcatattt gccttctctc gaaactaact gagcaaatgt ccctgcactt cttgcaataa
      481 ctgctccttg tcctggtctc atttcaatat tatgaactac tgttcccaaa ggaatatctg
      541 acaagaataa agcattaccc acttctggtg ctgcaccttt gccagattct attatctgtc
      601 ctatttgcaa accattaggg gcaacgatgt atcttttttc gccatcttca taaacaacta
      661 aagcaatacg agccgagcga ttaggatcat attctattgt ttttactcta gcttttacac
      721 catcgcgatc tcttttaaaa tcaatcaaac ggtattgtct tttatgaccg ccaccaatgt
      781 ttctaacagt catcttacct tgattgttac gtccaccaga ttttttcatt ggtaccaaca
      841 aagatttttc aggcgtagtg caagtaatat catcaaatgc actaatttgt ttaaaccttt
      901 gacctggtgt tgtcggattt atttttctta aagccatctc tattttttaa atattgctat
      961 aaaaatcaat tacttcacct tgtgccaatg taataattgc tttcttaaaa gcattggttt
     1021 ttcctcgttg aatgcctcgc ttggtgtagc gaacttttaa cttaccagca taacgcatag
     1081 tattaaccgc ttccacttta acatcgtaca tttgttcaac tgctttttta atttgcaatt
     1141 tttcggcacg tttatctaca ataaaaccat aacgattcaa agtttcgcct tgtttcgtca
     1201 ttttttcggt tactattggt cttattaaaa tattcatttt ttcttagttt taagctaaat
     1261 tattctcaaa aacatcaatt gacttctctt ccatcactaa aaccgaagcc cacataatat
     1321 cataagtgct taaatctgag gcagttacga catttacgtc ttttaaattt cgagaagaca
     1381 aatatacact tttatttgat tctgcaacaa ctaatgtaat ttttttatca ttaattttaa
     1441 aattgttctt taagttaatg atattctttg ttttaatctc atcaaacaac aagttatcaa
     1501 caacaaaaat tttattttct ttagcacgat acgacaaagc agattttcgt gctaattttt
     1561 taagtttttt atttaatttt atatggtata atcttggttc tggtccaaaa gttgtaccac
     1621 cgcctctaaa taatggattt ttaatacttc ctgcacgagc agttccagta cctttttggc
     1681 gttttatttt tctagtactt ccagatacta atccacgatg caatgtagcg tgagttcctt
     1741 gtcttttatt tgccatatat tgtttacaat caagataaat agcatgatcg tttggctcta
     1801 tggcaaatat taaatcgttt aattcgattt ttcttccagt gtcctcacct aacttattat
     1861 atactgatgt tttcattatc tttctataat taaatatgaa ccttttgacc caggaacaga
     1921 acctttcact aataaaagat tactttctgg aatcactttc aatactctca aactttccac
     1981 ttttactttc tgtccaccca ttcttccagc cattttttta cctttaaaca ctctagctgg
     2041 agttgaagct gctcctattg aacctggtgc tctttgtcta tctttttgac catgagactg
     2101 ttctcccaca cctgagtagc catgtctttt tacaacaccc tgaaatccct tacctttaga
     2161 aattccagct acgtcgcaat attcaccttc aataaaaatg tcaacagtta ctaaatcacc
     2221 aagttctaaa tcgctatagg tttcaccttt tttgatttgt ccaaaatctt taaactctac
     2281 caccttgcgt ttaggactag ttccggcctt tttaaaatga cctctcattg ctttggtcgt
     2341 atgcttttcc tttttctcgt caaaggcaag ctgaactgct tcgtaaccgt ctctttctaa
     2401 agtacgtaat tgtgttacta cgcagggacc agcctcgata acagtgcatg gaatattttt
     2461 tccgtcaaca ctgtaaatgg aagtcatacc tactttttta ccaataattc cttgcatttt
     2521 tctaattttt taagttttta acttgcttta atctcaacct ctacaccact aggtaattcc
     2581 aatttcatta gagcgtctat cgtttttgaa gtcgtactat aaatatcaag taaacgtttg
     2641 tatgagctta gctcaaattg ttctcttgac tttttattta cgaaagttga acgattaaca
     2701 gtaaaaatac gtttgtgagt tggaagtgga ataggacctc taaccactgc acctgtagtt
     2761 tttactgttt taagaatttt ctctgctgac ttgtcaacca agttgtagtc gtaagatttc
     2821 agcttaattc taattttttg attcatacta tttattatta ttaaaaaata aattttctat
     2881 tgttaattaa ctcttctaat attgttgcag gaactctttc ataatgagaa aattccatgc
     2941 taaatactgc tcttcctgag cttaaagttc ttaaagcagt aatataacca aattgttctg
     3001 ccattggcac tcttgctttt aagactctat gcataccgct catttccatt cctaaaatag
     3061 ttgcacgtct gcgattaagg tctgcgttta tatctcctaa atattcgtca ggtgttacta
     3121 tttcaagttt cattataggt tccaaaattg ttgatttcaa ctttttcgaa acttcagaga
     3181 aacataatcc agcagctatt tgaaatgcta aagcgtcact atcaacgcta tgataagaac
     3241 catcaactag ttctaccctc atattatgca ttgggtagcc tccgataggt ccattactca
     3301 tagcttgttt gaagcctttt tcaacagcat ttatatattc tttaggcagt tttccacctt
     3361 taatattatc aacaaattgc aaacctacaa aatctctatc atttacagga ccaatattta
     3421 cagtaatttc tgcaaataaa cctttgcctc cagtttgctt tttaaatata tagttataat
     3481 tattaatggt atcaataaat tcttctttat aatttacttg tggttttcct tgatttaatt
     3541 taagaccata ttctcttaca agacggtcaa ttattatttc caagtgcaac tcacccatac
     3601 cactaactaa agtttgccca gtattttcgt catgtctcag attaaatgtt gggtcttctt
     3661 cagccatttt agataaagcc tgagttaact tatcaatatc tgcttggctc aaggcttcaa
     3721 cagcaattga aatcacaggt tctggaaatt gcattttatc taaaataata ggctttgatt
     3781 catcacaaag cgtatctcca gttatagtat cttttagtcc tactacagca caaatatctc
     3841 cagcttcaac aacatcgatt tgagtttgtt tgttagaatg catttggaaa attctagaaa
     3901 ctctatcttt ttttcctgtt ctaacattat aaaccatttc tcctgatttg atttttccac
     3961 tatatactct taggtaagct aattttccaa caaaagagtt agaaacgatt ttaaaacaca
     4021 ttgcagacaa aggagcagat gcatcggctg ctctttcaat caaatcgtct gtttttggat
     4081 gatgtccttt tattgaatca atatctaatg gcgaaggaag aaaatcacaa atagcattta
     4141 aaagtagttg aatacctttg tttttaaatg ctgaaccgca taatatcggg atatagtttt
     4201 tattaattac taattttctt aaaaggtttt taaattcttg aactgatatt tttgatctat
     4261 cttcaaaaaa tctttccaac ataatatcgt caacttcaat aacagattct attagttttt
     4321 ctcgatattt aaaaacctcg tccttcattt cttcggggat atcttccaca cgatattttg
     4381 ctcctaagct atcatcatcg tcccaaacat aagctttttg ctcaattaag tctataacac
     4441 ctacaaactc gtcgccgtca cctataggta attgaattat taaaggttta gcattaagtt
     4501 tttcttctat ttgctgcact actttaataa aatctgcccc tgttctatcc attttgttta
     4561 caaaggcaat tctaggcaca ttatatttat ttgcttgata ccatactgtt tcgctttgtg
     4621 gttctactcc acctacagca caaaaaacag ctacagcacc atcaagaact ctcaaagagc
     4681 gttccacttc aactgtaaag tcaacgtgtc caggagtgtc gattatatta attttatagt
     4741 tttgttcatt ggtggtccaa aaagttgtag tagcagcgga agtaatagta attccacgct
     4801 cttgttcttg ctccatccaa tccatagttg cagcgccttc atgaacctca cctattttat
     4861 aattaatacc agtataatat aagatacgtt ctaccgttgt ggttttcccc gcatcgatat
     4921 gagccataat gcctatattt ctagtattta taagtttttg gttcatttac taaactattt
     4981 aaaatttaaa gtgagcaaag gctttgttgg cttcagccat tctgtgagtt tcgtcttttc
     5041 tcttaacagc agcaccttca ttattatatg cagccataat ttcagcagct aatttattag
     5101 acattgattg tccgctacgt ttacgagcgg cagctattag ccatttatga cttacagaaa
     5161 tttttctatc tgcgcggatt tcggttggca cttgaaaagt agctccacca atacggcgac
     5221 tcttaacttc tacagcagga gtaacattat ccaatgcttt tcgccaaata tcaataggag
     5281 catcttcaga atctttcatc tttttttcga taatttctaa actatcataa aagatttgaa
     5341 atgaagttga ttttttacca tcccacatca tatcattaac gaaacgagtt actctagaat
     5401 cattaaattt tggatctggt aatattattc ttttctttgg tttagatttt ctcatgttat
     5461 cttatatttt cctatattaa ttatttttta ccttttgtag cagctgcagc agctcctttt
     5521 ttaggtctct tagctccata ttttgagcga ccttgtttac gaccatcaac gcctgcagta
     5581 tctaatgcgc ctctaacgat atgatatcta acacctggta aatcttttac tctaccacct
     5641 ctaaccatta ctatagagtg ttcttgtaag ttatgacctt caccaggaat ataagcgtta
     5701 acttcttttc catttgttaa tcttactctt gctactttac gcattgccga attcggcttt
     5761 tttggtgtgg ttgtataaac tctcgtacac acacctcttc tttgaggaca tgagtccaag
     5821 gcaggagatt tgcttgtgta agtcattttt cgcctacctt ttcgtactaa ttgttgtatt
     5881 gttggcataa aacctaataa tttttaattt ttttaattca actacttttt tataaatttt
     5941 tggactgcaa aggtagatta atttttttta ttaacaacat atcttgataa aaattttaca
     6001 attttatcat tttattagac atttcaagtg tcaaacatgt aaaaatttaa aaatatcata
     6061 acaaaaaaac ttaaaaaatc ttacttattc aaaatgctaa aacaatatat ctgctttttt
     6121 ttcgttaaaa aattgcaaaa aataaaacta aggcattata tttgcaatta aataattata
     6181 taaataaaaa aacttattgc aatgaaaaat tatttttata ttttattaag tgttatagct
     6241 gttgttggaa tattttttac agcttgcgat cctgacgatg ataataaaga caaaaatgtt
     6301 atcgaaataa acgaagacat cacatctgct accgtatgga gtggcgacaa aacatatatt
     6361 attaaaaaat ttgactttta tgtaacagat gttttaacaa ttgaagctgg agcaataatc
     6421 aaatttagct cgcaagcaaa aagtttaact ctatctaaca atggacaaat attagcaaat
     6481 ggaactgctt ctcagcctat tgtttttacc tcaattaaag acgattcaaa aggtggcgac
     6541 actaataaag atggagatga cagctcacct gctataggcg attgggcaaa aatagatttg
     6601 aatggaacaa aaacttcagt ttttaattat tgcgaatttt actacggtgg ctttggcaca
     6661 ggagcaagtc ctaccttaga tttaagtgca gaatctgctg caaaaatcaa aaactgtact
     6721 ttcgctcata acggaggtgg tattaacggc aaatattata taggtacaat tcatgccgac
     6781 aatgcttcta gcgaaacagt tatccaaaac aacttgtttt atgacaacat actacctcta
     6841 actattacag gcgacataag cttagataat tctaacactt tttcaaaaga tggaacagct
     6901 aataaatata acggaatttt tgtctcaaga aatgttacta aaaacacaac ttggttagaa
     6961 gacgaaattg cctatgttat tacttctgat gaaatacaaa tacaacttaa cgcaagctta
     7021 actttaggag ataatgttgt attaaaattt tcggaagcta gtcgattaga tattgtgcaa
     7081 acagaaagtt ctttaggaaa ttacaatggc aatggcgtgt atttcacttc tttaaaagat
     7141 gacgaattaa aaggcgatac caatggagac gaaaatgcaa gtactccaca aactgccgat
     7201 tggataggaa ttcacattaa tggctggaag tcaacagggt atgctaattg gtccaacatt
     7261 agatataatg atcctaatcc agcagttaaa taaacctagc taacaaattg caaccttttt
     7321 atcgtttaat tgtaaaatta aaataatcaa aaatatttta cgggtaaaaa gcttctatta
     7381 tatgctctat ttccattcct gattttaagt aaagcacaga tactacatca aatctactat
     7441 tgcctgtaaa tccggtttcg gcaatataag cttcggcggc atttattaat aatttttgtt
     7501 tttttctact tacaaattct tctggattgc cccaaaagtt ttcactacgt gtttttactt
     7561 caacaaaaac taaggtatcg tcgtccatgg caataatatc tatttcttta tgcttatccc
     7621 tccaattagc agaaattatc acatatcctt tttcagccaa catttctgca gctaaatctt
     7681 cgcctttatt tcctaagttt atagtttttg acataagcag ataaaaaaaa tcccgaagtt
     7741 tattttcggg attaaatata caatattttt tattaattta gtgagcgaca actagtttta
     7801 ttgttttttg aatttttcca tcattatata aacgacagaa ataatatcca ttatcccatt
     7861 tactagcatc gatattcaca ccattgacaa aatctgattt tacttttgta accaattttc
     7921 caagcatatt ataaacttca actacagcat taggtccgta attatgttta attttaaaat
     7981 tatcacttgc tggatttgga aagatagaaa attctttaac aagttcacta gaaatagaac
     8041 ttgggaatcc tgtactaaat ataattataa aagaatctgc aaaacctcca ttaacatcag
     8101 caacttcaat ttttataata gcttcacctg aagtagaacc ttgtgtaaaa tttatactac
     8161 caggtgttga tgtcgtttca tttgctgcta aagttatttt tgcacttgga aatccaggat
     8221 tactacattg acccccacca gctgcaaaga ttggagaaca aaaactaaca acccagtcat
     8281 ttattgttcc accttcacca taagcgtaca ttttaacttc aacttgatta ctagtgttat
     8341 tttttaaatc aactttatat tcataagtag tacctggagc aacattataa acttttgaag
     8401 ttgtgtttgt taccacacct gtactatcaa taatatctat agtttgagaa gataaaatcc
     8461 caacaactaa gaacaaacta aaaactaaaa gtataattct tttcatatct acaatttttt
     8521 acaaaaataa attaaaaaaa taaaacttca aagaaaaaaa ctcataatat tatttttttt
     8581 cgaaaaaatt aaatacttta aaatataaaa tacaacaaat acaaattaaa gcaattttat
     8641 cattttttca ttagataact ttaaagcaat tagctacaac aacattttgc attttgtatg
     8701 ttctatttac cattttaacc taccataaaa aacattagtc taatttcaat tttcattaac
     8761 aagctaacta aaaaataata acctattttg cataattaac ataattaaaa taaataactt
     8821 ataaatttaa aatgaaatat tatgaaaaga tcgattttat tatttgcaac acttttgttt
     8881 gtgtttgcaa gctttgcaca aaataaagat gcaaacacat cacctatttg tcaagagccg
     8941 acaaatgata gaggtactca agcattacct tgggcagaaa tgcaaatgtg gacagcaaca
     9001 gatattaaag gcaacaccca taatcttgca gattatctag ctactggcaa aactgttttt
     9061 gttgattttt ccgcaacatg gtgctaccca tgttggagtt ttcaccaatc aggtgtttta
     9121 gaaaaccttc acaatcaatt tggtcctgga cccgacggca caaacgaaat agtagtttta
     9181 tggttcgaag tcgatggtgc taccatagag caaatacacg gtacagggtc taatactcaa
     9241 ggtgattgga ctgaaaatga cacatggcca gttccaataa ttcaagcatc tagtcaacta
     9301 aaaagctatt tctcagaact atatcctggt gcagttccta ctatgttcat gatttgtcca
     9361 acaggctatt atagaacagt tgatgcatca actagtgttt cttattacat gtcacaaatt
     9421 aattcgtgtc ctgaagcagg ccaagtacct ttagcagata ttgatggtag cgcaatagct
     9481 tttatagata cgcctagcac ttttcaagct gatgctgtta gtatagatcc tataactggt
     9541 tacgaatgga cttttgaaaa tggagatcct gctacatcag accaacccaa tgtaggtgta
     9601 acttggaaca ctcctggaca aaatataatt tctttaaaaa ttcataacga aaacggacct
     9661 agttctccaa aatataaaac tgttaaagtt atagaccctg cgactattga taataattat
     9721 ttaacttttg aagaaatacc tgtaggaaat acttacccta aaaatttata tccttattcg
     9781 tggactagtg taaatagaga caatggaact gtttacggca gtctaactaa ttatggggta
     9841 tcaggtaata atatgtcttt tgttgtatat agcaaatcag caacatctcc ttcacattta
     9901 aatgttgctc ctaaaagtgg agacaaatgc gctttggcaa tgacaaatgc ttctggtgga
     9961 acaaacaacg actggttaat atcgcctaaa attcaactcg gcactaactc atcgtttgaa
    10021 gcctatgtaa aatctctaac tgctaattac ggattagaaa aatataaaat aatgatttct
    10081 acaaccaata cagaagaagc tagttttaca atgttgggtt cagtatcaac agctccagtt
    10141 aattggacaa aaataacaag agatttaagt gcttacgacg gacaagaagt ttatatagct
    10201 ttagtttatg ttggctatga taactttata tttttcgctg atgatataaa aatcaccact
    10261 actccaacag ctatcgaaac aaataactta gagttagaag ctcgtatttt cccaaatcca
    10321 gcaaatgatg tcataaatat taattatgta gaaggtgcta aaattcaaat cttcaacaat
    10381 attggacaag aagtttacag tttggctaat gctaacgaat acaaccaaat tgatatgagt
    10441 gctcaccaag caggctcata tgttgtaaga gttacaaaag gcgataaaac taataattat
    10501 aaaatgatac ttgtaaaata agattaaata atttttactg tttttaatct ttggctcaaa
    10561 aattgcttaa taacttaaaa cagtttattt ttacaaaaat ttaaaattat gaaaaggttt
    10621 attttgatta ttagtttaag tttattcatc agttctcttt ttgctcaagg agttccttca
    10681 gtaatgatta aagatataaa aggtaaagct gtaaatacaa aggctattac ccaaaacgat
    10741 ggaaaacctg tgttgatttg tttttgggca acttggtgca aaccttgtat caaagaattt
    10801 aatgccatta acgaagaata tgatgattgg gttgatgaaa ctggtgttaa agtaattgca
    10861 atctctattg acgatgctag aagttcaaat tcagttgctc catttgtaaa tgcaagaggc
    10921 tgggaatttc ctgtttatct cgaccctaat ggagatttta aaagagctat gaatgttggc
    10981 gatattcctc atactttttt attagataaa aatggaaaag tagtttggca acatacaaca
    11041 tatctcgaag gcgacgaagc taaaaccttt gaggtgatta agaaagttgc taaggggcaa
    11101 gcaataaaat aaaaacataa tcaaggtgtt tttattttga aagaatttat aagatttaaa
    11161 ataatgaaaa taggcggtgc ctcaaaacaa gtgttaagac aataatattt caaggtgttt
    11221 cagaagtgtt aaaatgcaag gcgatgaaat tgaggacgaa ggagcgtacc agaagcgatg
    11281 cactgagcaa agccgaagtg tacgtgactg agtccgagta ttgaaatcaa cgtagcagtt
    11341 tgacacttat gagacaccgc ctatttttgt ataataattc agacaactca aaattacatc
    11401 taatgaaaaa aattatttta ttcattttaa ctgttttgct tttaaaccat gcttattctc
    11461 aaaatgagac acaagcaaat tatggaagta tatctgggaa cttccaaagt gaagctcaat
    11521 attacattac agactcgctt atcggtgcta cagctccaat agaaaagact ttagccaatt
    11581 cttgggcgaa tataacttat actctaaaaa acttttcagc tggagttaga tacgagggct
    11641 acctaaactc tattcttggc tatccaaact ctggcggaag aaatgatgga attggatttc
    11701 ctcatcgttg ggctaatttt gttgtggaca agtttgagtt tactgttggt aatttttacg
    11761 aacaatttgg aaatggctta gtattaagat cttatgagga aaaaagacta ggtgttgaca
    11821 attctcttga tggttttaga gtaaaataca atcctgctca aggtgtttat ttaaaaactt
    11881 taatcgcaaa acaaagattt tactgggaac acggtcctgg catagttaga ggttttgatg
    11941 gcgaaattca gtttaatgac ttgattgaaa aatttgccga aagcgattta agaattagtc
    12001 ttggaggtag ttttgttagc aaatttcaaa aagaggaaag tccaatttat aacttaccaa
    12061 aaaacgtcgc tgcctctgcc ggaagaattg acattagata taatggtctt gctataaact
    12121 cagaatatgc atataaaata aatgacccag gtgctgataa taattatatt tttaaaaacg
    12181 gtcaagcttt tatggtaaat gcaagttatt cgcaaaaagg tttaggaata gttttaagca
    12241 caaaatgggt tgacaatatg gcttttagaa gtgatagaaa tgcaagcctc actgatttga
    12301 atataaatct actcccagaa ttaactagaa atcatgttta tatgctacct gctttttatc
    12361 cttatgcaag ccaaccaaaa ggagaatggg gagcaaaagc agaaattatg tacaaaattc
    12421 caaaaggcaa tgtgctagga ggaaaatacg gaactactat tttggtaaat tattctagag
    12481 ttcacaatat tgacaaacag gcaattaacg acactctgcc tttagatgct ccaggaactc
    12541 ttggttataa tacttcgttt ttctctattg gaaaagaact ttatttccaa gatgcacata
    12601 ttgaaataaa taaaagatgg actaaaaaac tttatacaaa tctttcatat gtgaacttat
    12661 tatacaatta taatatacta cgaggcacaa caggacatga taatgtaaac gcacacattg
    12721 caatagctga catcacttat aaatttaaac ctcaattagc tttaaaatca gaatttcaac
    12781 atatgtacac aaaacaagac gaaggcagtt gggctatggc tttatttgaa cttactacct
    12841 ctggttggtt ttttactgtt cttgataatt ggaattatgg aaaccctgac aaaaaccaca
    12901 gacctcatta cttcaatgtt ggctttggtc atatttcggg agcaacaaga atacaattaa
    12961 cttatggaaa aaccagagct ggtgtaatgt gtataggtgg tgtatgtaga aatgtacctg
    13021 cttctaacgg aataagttta tcaatttcat caacctttta aattagaata atatgaaatc
    13081 aaaatatttt aacataataa tacttagtgc atttgtagca ttattttttt cttgcgacga
    13141 aattgaagga ccatatataa aagacgaagc tacaatttgg aatggcaaaa aaactttaat
    13201 tcttgatttt acaggacatt gttgcggaaa ctgtccaaga gcacataaaa ccattgatga
    13261 gttagttgaa aaatttggcg atgctattgt tcctgttgct gtacactcaa catattttgc
    13321 aatgcctgaa actaccgaca cttcaaaacc ttttcactac gattttagaa caagttttgg
    13381 cgatttctgg ggtggtagag attatgcatt tgggcatctt ggcgaaatat atttacctac
    13441 aggattagta aataacctaa acccagacca agtaacaaac acaaacgctt gggcaggctt
    13501 aatagcaagt gttttagcaa attaccccga gtacaatata gaaatttatc caagctactc
    13561 aaactcagac agtttagcta aagctgatat taaaattatt acaaacatta aaaatcatag
    13621 agaacttagc cttttagttt ttattgttga agatggaatt attcaatggc aaaaagatta
    13681 ctctgcaaca cctaacaaca taaaagaata tgaacataat catgttttaa gagcttcgtt
    13741 taatggaaat tttggtgata aaattaaaga taacaatgcc gaaacaatta ttggagacga
    13801 aataaataaa tcttattcat taaaaatcaa taaggattgg aatattaata attgtagcat
    13861 agttgcattt gtttatgata ctgagactaa agaagtttta caagcagaaa caaaaatagt
    13921 aaatgaataa atttctaatt ttaatatttc taacactatt ttcaatttca ttaaatgcac
    13981 aatcatttta tggtggaata attgttggca caactctctc tcaagttgat ggagatgact
    14041 atggcggttt tcataaaatt tcacctcttg gcggagttta tgtaagaaac acttataatt
    14101 ccaattgggg aacttcctta ggaattgaat acaaacaaaa aggcagtaaa gatgtttcaa
    14161 aagacgaata cggttatgtt acaaattatt acgccatgag acttgattat attgaagtgc
    14221 cttttttagt aaattataaa ctaaacaaat ttaaaattcc taaacttgtg gattacagtt
    14281 ttaaaaatga cttttggatt gattttggag tttctggagc atatttaatt aaaggtactg
    14341 atgattacgg acatggtcct actctacctg taagagaatt tagaaaatat gaagtcgctt
    14401 gtcattttgg tatcaattat tattttaatt ctcattggat ttttcacaca agattttcat
    14461 acacttttcc acttttgcca attagaaaac accctggcgg acaagtttat tggctaaatc
    14521 gtggacaata caatcacaat ctaagttttg caataaaata tgagttttaa tggattctaa
    14581 gaaaaaaaat attctagtcg caattactgg tggatcgggt gctatttatg caaaacaact
    14641 tttagaaaaa ctaaacgatt taaaagaaaa agaaagctta gaaatttctg taatctactc
    14701 taaaacaggc aaagaggttg ctattcatga aattggttac gattttgtaa aagaacttaa
    14761 tttaaaaact tacgaagaca ataattttta tgctccattt gcttctggtt cttctcctgc
    14821 cgatatttta gtaattgctc cttgtagcat gggaacttta ggacgcatag ccaatggttt
    14881 ttcagacagc ctaattacca gagctgcaga tgtagtttta aaagaaagaa aaaagctaat
    14941 tttattaact cgcgaaactc ctttaaattt aattcatatc aaaaacatgg agctaataag
    15001 cctaagcgga ggaattattt ttcctgcatc accttcattt tataacaagc ccacaaatat
    15061 tgaagactta gttaatactg ttatttttag aattttagat ttaatgcaaa ttgaaataac
    15121 ctacgataga tggggaaaat cagttaaaag tttataaagt tcgtaaagtt cataaagttg
    15181 aaccgagctt gcgaacttac agaaggtaaa gagtttttag tgttgagttt ttagtgttta
    15241 gtagtgagtt tttaaaaact tcacaatgat agataaaatt tctttaatgc aaagtttgtc
    15301 tgtacttgct tcgcagagtt cgcaagctca ctctgcttgc tccaacactt atcgaatttt
    15361 taaaggcaaa agttttttca gtttttctaa atcccaaagg caaaacaaca ttgcatataa
    15421 aaatggcaaa cctatgcttc tgtatcttac tatagcacca gaattaggag tgtaagcacc
    15481 aactaaaaga aataaataca gcacaaaacc taaagaaaac aaaacaaaaa gcattctatc
    15541 tccatcaggt tttttaaaaa atattatagt aaaaataaga actaaactaa aaaacaaaac
    15601 ctctaaaaac gataataata taactggtga attaatttca tttgggaaag gtctaaaaaa
    15661 gctatttagc aatccttcgg gaattatttt taaaaaactt tttacgtttg gttctaagcg
    15721 agttaactta aaagtacttg tttgcccctc ttctaattca gtataattga taaaatcatt
    15781 ttgctttcca gcaatagctg ccattatgtc ataacctata attttatcgc ttaaaacaat
    15841 agccataaaa ccaagtaaac ttatagaaat aagaattaat actcgtcctc ttgttttaaa
    15901 tctttttgca ataaaaaaga aaacaaccat tggcaaaaac actggtaaga catgtatttt
    15961 cgaaattcca accaaaaaca aaaagaataa aaacaaaata aaatcaagta tcttaaattt
    16021 aaaataaatt tttaccatat aataaaaagc aaagcccaaa gcaaacatta ccaagccttc
    16081 ttttaacagt cccgaagtcc aaaacaaaca agacggaatt aaaaaacatg ctactacatt
    16141 caaatattta ttcaagcctg ttagtttgtc aatggcttta aacaaagcaa atgccccaag
    16201 aaacgacata aatgccattg ttataatatg aataaaaata tttccacgtg atactaaaca
    16261 caataaagca ttaaacctca ttattgttcg cgaatcgtta aacaatccat aagaattttg
    16321 cctagtccaa tggtcggttt ggtcataata tttttcaaga ttttctctat cgccttgaat
    16381 acctgttaaa agtctaaagt aatcagcata agattcttct gttgccgaat gtaaaacaca
    16441 accagctcta aaatatttat gaacatctga agtttttttg tcttcataat aatatgtata
    16501 tataagatat aaagacaagc ctccaataaa ctttaaacta aacaaaatag ccaaaatata
    16561 aatcttaaat ccagatttgc gaaaaaactt caacttaaaa ataagtacta taaaaagcag
    16621 aaaacataat atggcaaaaa catatgtcat tatgaaagaa taaagttttg tttatttttt
    16681 tctatattat attttgggtt ttcaggattc aatttgggtt ttcaggattc aatttttctc
    16741 tagaaattac tttttcatat ttcttcaaat attctgttgc cataactttt gcattaaaac
    16801 aatctctagc atattctgaa atatgattag aattataatc agcataattt tttattgctt
    16861 gggataaatc ttcttttttt gaacttaaaa agccaaattc tggttttact aattctggca
    16921 acgagccata aggcgttcca aaaa
//
LOCUS       DAOPNX010000057        16784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_4097_length_16784_cov_5.581386, whole genome shotgun sequence.
ACCESSION   DAOPNX010000057 DAOPNX010000000
VERSION     DAOPNX010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16784
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4097_length_16784_cov_5.581386"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..698
                     /locus_tag="PKN53_07350"
     CDS             48..698
                     /locus_tag="PKN53_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HOD88802.1"
                     /translation="MTSIKKLADEILVNPEFIDLNFSLPAKEIDQKVFMVYESDKPRL
                     LKTILSDFKDEKVLIFAGTKLSVSKISSYLRSCSIKHSSINSNFSQDERNETVQNFKS
                     GNFNILVTTNLLARGIDIEDVNLIINYDIPEKAEDYVHRIGRTARAGKLGLAISFIAE
                     KEILSFNRIEKLLKREIPKNPLPDGFGKSPEYKIILKPKRRKWKPRKNFKKNKNKK"
     gene            752..1507
                     /locus_tag="PKN53_07355"
     CDS             752..1507
                     /locus_tag="PKN53_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88803.1"
                     /translation="MSGFFKRNINGILGTILFHLIVVVVTMSLRLAEPRPNNEEYFIV
                     ETEFLEEMTKTEKDILKDENSLEDFDIENYLKEIRNVGANRQDNRYSNIDALSEEELK
                     QKYESELLKEKYGSDYEKRMNLSHEDFLKNKDNPTKDKNKSDANANTSSNAVNYSGPA
                     LVYVELQNPNRGKSYIHVPVFTCENSGRVVVGISIAPDGSVRSANIVSTKCSGDATCI
                     SNAAREAALKSKFSAIAGGKTETGTITYSFIEQ"
     gene            1673..3295
                     /locus_tag="PKN53_07360"
     CDS             1673..3295
                     /locus_tag="PKN53_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOD88804.1"
                     /translation="MIKENLVAYFEDAILNNWDTMSLRDYDSGNGLTYGELGNNVYNL
                     HKLYRDCGIKKGDKIVVIGKNSANWGLVYIATVTYGAVIVPLLQDFMPKDIYTLTNHS
                     DAKLMFASEFICQKFDYSNFEQLQSIYYLDNFEIAHDFSNKNIAYDNNPNATSKYNFK
                     LEHIPNPEIAAILYTSGTAGQPKGVMLPHNSLAANVKYARPNMPLESGDQIVSFLPLA
                     HCYGAAFEFLYPFSLSCTITFLGKLPSPQVILKAFNEIKPKLVLSVPLVVEKIYKSKI
                     KPTIDKPSIKFLLKLPIVKGILHKKIRDSLYQAFGGNAKEIVIGGAKLNREVETFFRK
                     IGFNFATGYGMTECGPLITYDGWRTTKIYSAGKVVDHLEMKINSTQEGKIGEIFVRGE
                     SVMAGYYKNEEETANAIDSENWLHTGDLGLIDEDGYLFIKGRSKSMILTSSGQNVYPE
                     EIEAKFYNFLLISEILVVSRNGVITALIYPDQDRIKESHLKPEQVEKILNHYIKHVNE
                     DLPAYMQVKKLVIREEEFDKTPKKSIKRYLYE"
     gene            3524..4666
                     /locus_tag="PKN53_07365"
     CDS             3524..4666
                     /locus_tag="PKN53_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HOD88805.1"
                     /translation="MKKILYLIIIVNSLFLQSLFSQELSQNAVVSLITQDEGDELYAY
                     FGHTALRIKDDSLGIDLAFNYGTFDFSTPNFYTKFAKGTLNYCLSVETYESLVFSSSH
                     NKRTLREQVLNLTYEEKLKTYQLLVNYYNSEARYYRYDFFYDNCATKIRDIIQEATNN
                     RIDFSKSNFKGHSYRQLIMPFLEKNYWIDFGINFILGLEADKKVPPEQYMFLPVYIHD
                     FLEGSDFAQNSQVVLDASPAKKQGIDFGYILPWLIVLVWGILIYFKKTRGFFKYLLFT
                     IFGLLGLFILCFGLYSEHTAVLYNLNTIWSLPALLILIFSWSKKWLHYIKLGYLIILL
                     LHLINWFWLPQQMSTTFLPWMCLLIFTLLVDLNYFKYFKKRKATKN"
     gene            4796..5347
                     /locus_tag="PKN53_07370"
     CDS             4796..5347
                     /locus_tag="PKN53_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88806.1"
                     /translation="MEIHTHTFFNESVPTTFKKIAESLGEEVTTEMIAPGGYRFSNHC
                     TNQATLDLINSRNWDYVVLQEQSQLPSFPPAQVANEVYPYAASLCNTIRQNDICSQII
                     FFMTWGHKNGDASNCPYYEPLCSYEGMQWRLRQSYVEMAEDNQAWVSPVGMAFAQIRQ
                     SNPEIELYSSDNSHPSAYGTYLG"
     gene            5460..5657
                     /locus_tag="PKN53_07375"
     CDS             5460..5657
                     /locus_tag="PKN53_07375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD88807.1"
                     /translation="MYPNPSLDKIIFVENAENQVCSIFDLAGRKICQKEIKDKTLNLT
                     DLANGMYLIEINSETFKIHLK"
     gene            complement(6134..6208)
                     /locus_tag="PKN53_07380"
     tRNA            complement(6134..6208)
                     /locus_tag="PKN53_07380"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6171..6173),aa:Val,seq:gac)
     gene            6781..7182
                     /locus_tag="PKN53_07385"
     CDS             6781..7182
                     /locus_tag="PKN53_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88808.1"
                     /translation="MILIAILMASSLFSKQVTIIKEGGKLNKDGTVTYDDIKQQFTNG
                     ELTYQHCLRRGHEKCPKVGIVTIGTGVSIDKYIELAEEVIAKNLEKGITTGKISYPSD
                     NIMIYYKEASFVLDENGKETDKIEYHMTIEY"
     gene            7226..8704
                     /locus_tag="PKN53_07390"
     CDS             7226..8704
                     /locus_tag="PKN53_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88809.1"
                     /translation="MLKKFTLLLLTLIIGKLMFCQHFNVIKTIELETLYGEQFYAYYD
                     RIQSENKTIIPIYPIYEDDFENMIYVVIDSLDNFSLLQWKREADKDGYKDTPIAGIFL
                     DNNKGILISEYLYYFQIKGKQMLTTKKIKPPNQSKYGEFYEIYPLGDNSSKYVLLANT
                     YNCSSRKTIYDIFRFAKFNIKKKKIEKIINIDVGKGIFLSHFSNHMISTSKNNIAISH
                     PCKPEVYLFDNDLNLLDTIVIDYPNIFKTKNLLDSILTDTLILENRYMTINLLQILHN
                     NNIMKYPRIDKVAFIDEDLLFIFVKPDAVVDTNNNWHRNHLHYVYSLSNKDFFETKGK
                     ISNFDAINSSQPLIFHNNKTVAVWDSVDTNNLFKYYISFCEISIDFNSKYIDTIDLSI
                     LPPFLHLKSIDSLNKNQNYEKFNYIMVANYAACSHCKFYDKYPNLMIIHVFDKKDAYN
                     RSTMLSYKTKYNKMFDNPTVYFCEDSMIDLNKIKINAIYKTK"
     gene            complement(8977..9924)
                     /locus_tag="PKN53_07395"
     CDS             complement(8977..9924)
                     /locus_tag="PKN53_07395"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOD88810.1"
                     /translation="MKSFCVLGLFLISTLPFLPFNPYTVTPPGTTRLNDSLFVDICEL
                     SVSDWNEYSFWLKRMYGEDSDEYKACFPDSTVWRKSIYEQVAKSYFKHPAYYNFPIVG
                     ITYEQAVAYCEWRSIQVNYMLYARQNKLAYSLKDDTVGIPQIYKYRLPSKQEWEVIAS
                     LGFSKKTLKQIEKSYNPNIYNLIYPRHQGENATNAINLNTTAKVNSFWTNLIGCYHIF
                     GNVAEMTDTKGLAKGGAWLHNAYEVTVDRDFYYKGSQDWVGVRCVCEKVMKSEKEIAR
                     DSLRQAKLERKFQRDSIKQEKLLQKQERKRGDDSDEDDD"
     gene            complement(10008..10511)
                     /locus_tag="PKN53_07400"
     CDS             complement(10008..10511)
                     /locus_tag="PKN53_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOD88811.1"
                     /translation="MRNRISKILQEEGMTASKLADEIGVQASSISHIMSGRNNPSIDF
                     VQKLLERFRAINSEWLLLGKGEMYKQSQGTISSNLSSSKPIENDLFSQASYEEDKYSN
                     QEPLKTVDNEPKNLEEATKTLDKDKFVDEPKEMLKEINSSSKEPKTVEKVIVLFNDGT
                     FEYYNQA"
     gene            complement(11930..12379)
                     /locus_tag="PKN53_07405"
     CDS             complement(11930..12379)
                     /locus_tag="PKN53_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOD88812.1"
                     /translation="MTRDQAHDGSKRMHPVIHVHILNSKGEVLLQKRNENKFIQPGKW
                     DTAVGGHISHGENLETALKRETYEELGLKNLKYQYITKYIWESEIEKELVFVFLAQHE
                     EFNFVKNDEVDEVKFWSFKEIKKNLSKNVFTPNFEVDFINILNKIKK"
     gene            complement(12497..12997)
                     /locus_tag="PKN53_07410"
                     /pseudo
     CDS             complement(12497..12997)
                     /locus_tag="PKN53_07410"
                     /inference="COORDINATES: protein motif:HMM:NF016189.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
     gene            complement(13159..13329)
                     /locus_tag="PKN53_07415"
                     /pseudo
     CDS             complement(13159..13329)
                     /locus_tag="PKN53_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150394.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(13385..14725)
                     /locus_tag="PKN53_07420"
     CDS             complement(13385..14725)
                     /locus_tag="PKN53_07420"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOD88813.1"
                     /translation="MKNIILLILVFLISKTHFSQEVIDQVVAVVGNEIVLKSDVENQY
                     IQLRAQGTYDLNGDLRCDILEELMFQKLLYLQALQDSVEITDKEIESELDRRISVFVN
                     QLGSEKKMEEFYGKSISEIKKDFKSIIHEQLLTQRVQAGLVSDIKITPSEVKEFFEKI
                     PEDSIPMVSAFFELSEIVFAPEIGKEEKDATIQKLNDLRDRILNGESFQTLAILYSED
                     PGSATRGGELGFVSRTDLVPEFASVAFNLTSPIEVSRVVETEYGYHIIQLIEKKGNLM
                     NFRHILLTPEVSIEQISLTEEKALEVYELLQKDSISFSEAVTKYSDGETKFNNGKVIN
                     PYYGTTKLANEFVDPNTVRNIAGLKVGEYTKPFLSSSSKGAKLIKIVRLDQKVEEHKA
                     NLTDDYLEIQEYATQQKGQKIIEKWIHNKLESTYIRIDKTFCDCEFKFGNWKNE"
     gene            complement(14736..15578)
                     /locus_tag="PKN53_07425"
     CDS             complement(14736..15578)
                     /locus_tag="PKN53_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88814.1"
                     /translation="MRFFALLINIAILILLSSCANKNKEPQGKIIVKYGNEYLLEEDI
                     IKKLPKFNSQTDSINYVNSLIDAWLEQKLFFDEAKIKITDSSKICRKIEDYRKNLYVN
                     EYLNNHVYNSINDKVSRDEIEEYYNKYLDNYKLSNTYVKAHYMTILDPKASHYYEYET
                     VRKSSLEDEKELIDYCIGTGRKVYFLKDWIELDDFLEIIHYQGDINGFSQNQNFVNYV
                     YDDMRYLVKIDEIITEGDTMPLELAETLIYQVIMKNRKDSKYQQVKNELKTNAINSGK
                     LVIN"
     gene            complement(15580..>16784)
                     /locus_tag="PKN53_07430"
     CDS             complement(15580..>16784)
                     /locus_tag="PKN53_07430"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOD88815.1"
                     /translation="KINEVYQKAIAGEDFAQLADEYSEDRRTATNGGMIGWVSVGGRM
                     IKEFEEAVFKLENIGDIAPVLKTNYGYHVIKLVDKEEIKPFDEAKNDLKRLISNTART
                     SKSRDAIVNTLKNEYNAKTIDENLKLFYTLVTDSIFNGTWAVDENLKLDKPILTFANQ
                     TKTQKDFVDYLMKYNRKQSPQDVKMFVDNAYNNFVSKMILMYEEDILENKYPDFRYLI
                     KEYHDGILLFELTDRFVWSKAIQDTVGLEKFYQENKNSYMWDYRYDVRVYECKDAKVL
                     NSAMKLFNKKKSEEFILAKINKKDSTAIVLSESAFAEKGVKPLVDSKIKQFNISETDG
                     FRLVSQGENNTITLIEVHGPEVKTLNEAKGIITADYQNYLEKEWIKTLRSKYNIKVHK
                     DVLKTLAN"
BASE COUNT         5969 a         2537 c         2208 g         6070 t
ORIGIN      
        1 tctttaccac aaattaatac aagttatcat aaacaacctt gtgaaacttg acatctataa
       61 aaaaacttgc agacgaaatt ttagtaaatc cagagtttat agacttaaac ttttctttac
      121 ctgccaaaga aattgaccaa aaagtcttta tggtgtatga aagcgacaaa ccaagattgc
      181 taaaaacaat attaagtgat tttaaagatg aaaaagtatt aatttttgca ggcacaaagc
      241 ttagcgtatc aaaaatttca tcttatttaa ggtcatgttc tatcaaacat tcttcaataa
      301 attcaaattt ttctcaagac gaaagaaacg aaaccgtaca aaattttaaa agcggaaatt
      361 ttaatatttt agttaccacc aatttgctgg cacgtggaat tgatatagaa gatgtgaatt
      421 taataatcaa ctatgatatt cctgaaaaag cagaagatta tgtccacaga ataggacgta
      481 cagccagagc tggtaaactt ggtttagcaa ttagttttat tgccgaaaaa gaaattttaa
      541 gttttaatag aatagaaaaa cttttaaaac gcgaaattcc aaaaaatcct ctacccgatg
      601 gttttggcaa aagtccagaa tacaaaataa ttttaaaacc taaaagacgc aagtggaaac
      661 ctcgtaaaaa tttcaaaaaa aataaaaata aaaaatgact tatatagttt tatatttgta
      721 aaactttaaa tcttaattta attttgcatt tatgtcaggc ttttttaaac gaaatataaa
      781 cggcattctt ggtacaattt tgttccattt aatagttgtt gtcgtaacaa tgagcctaag
      841 acttgccgag ccaagaccaa ataatgagga atattttata gttgaaactg agtttttaga
      901 agaaatgaca aaaactgaaa aagatatatt aaaagacgaa aactcacttg aagattttga
      961 tattgaaaat tacttaaaag aaatacgaaa tgttggtgct aacaggcaag acaacagata
     1021 ttctaacatt gatgccctaa gcgaagaaga attaaaacaa aaatatgaaa gtgagctttt
     1081 aaaagaaaaa tatggcagcg actacgaaaa aagaatgaat ttaagccatg aagatttttt
     1141 aaaaaacaag gataatccta caaaggacaa aaataaaagc gatgccaatg caaatacttc
     1201 atcaaatgca gttaattact ctggtcctgc tttggtttat gtagaattac aaaatcccaa
     1261 tcgtggtaaa agctatattc acgttcctgt atttacttgc gaaaatagtg gacgagttgt
     1321 tgtaggaata agcatagccc cagatggtag tgtaaggtct gcaaatatag tttctacaaa
     1381 atgtagtgga gatgccactt gcattagcaa tgctgcaaga gaagctgctc taaaatcaaa
     1441 attttctgct attgccggcg gaaaaactga aactggaaca attacatatt cttttataga
     1501 acaataaata attaaaataa aacatatttt aattttatac gtttgtatgt atttattcaa
     1561 aatacatcga aaaatttact tcgtttatca aataacaact gcctatttta atatgtttaa
     1621 aaaattaatt atttttacac aaaaaattac acaacaataa aaactgcaat ttatgataaa
     1681 ggaaaattta gtagcttatt ttgaagatgc tattttaaat aattgggata caatgtcctt
     1741 gcgagattat gatagtggca atggtttaac ttatggtgaa ctgggcaata atgtttacaa
     1801 ccttcacaaa ctatatcgtg attgcggaat taaaaaaggt gataaaattg ttgttattgg
     1861 caaaaactct gctaattggg gtttggtgta tatagcaact gtaacatatg gagcagtaat
     1921 tgttccatta ttgcaagatt ttatgccaaa agatatttat acattaacaa accactctga
     1981 tgctaaatta atgtttgctt cagaatttat ttgccaaaaa tttgattatt caaactttga
     2041 acaattacaa tcaatttatt atttagataa ttttgaaatt gcacatgatt tttcaaataa
     2101 aaacattgca tacgacaaca acccaaatgc tacatcaaaa tataatttta aattagaaca
     2161 tatcccaaat ccagaaatag ctgcaatact ttatacttca ggtactgctg gtcaaccaaa
     2221 aggcgttatg ttgccacata attcattggc tgcaaatgtt aaatatgcaa gaccaaatat
     2281 gcctttggaa tctggagatc aaatagtttc atttttacca ctagcccatt gctacggagc
     2341 tgcttttgaa tttttatatc cattttcttt aagttgtact attacgtttt taggaaaatt
     2401 accttcacca caagtaattt taaaagcatt taacgaaata aaaccaaaac ttgttttatc
     2461 tgttccactt gttgtagaaa agatttataa aagcaaaatt aaacctacta tagataaacc
     2521 ttctattaaa tttttactta aacttccaat agttaagggc atcttacaca aaaagatacg
     2581 tgattcttta tatcaagcat ttggtggtaa tgcaaaagaa attgtaattg gtggagctaa
     2641 acttaataga gaagttgaaa catttttccg taaaatagga tttaattttg caacaggtta
     2701 cggaatgact gaatgtggtc ctttaattac ttatgatggc tggagaacta caaaaattta
     2761 ttctgcagga aaagttgttg accacttaga aatgaaaatc aattcaacac aagaaggcaa
     2821 aattggcgaa atttttgttc gtggcgaaag tgtaatggca ggttattata aaaacgaaga
     2881 agaaaccgca aatgctatag atagcgaaaa ttggttgcat acaggcgatt taggtctaat
     2941 agacgaagac ggatatttat ttataaaagg acgaagcaaa agtatgattc ttacaagttc
     3001 tggacaaaat gtttaccccg aagaaataga agctaaattt tataattttc tgcttataag
     3061 cgaaatcctt gtcgtaagtc gtaatggcgt aattactgct cttatttatc ccgaccaaga
     3121 tagaattaaa gaatcacatt taaaacccga gcaagtagaa aaaattctta atcactatat
     3181 aaaacatgta aacgaagatt taccagcata tatgcaagtt aaaaaacttg taattcgtga
     3241 agaagaattt gataaaactc ctaaaaaaag tataaaaaga tacctgtatg agtaaactca
     3301 gttaaaagtt aataaagttt ttaaagttca taaagttgaa tacttttagc cctattctgt
     3361 tataatttga ttttttaact gaattattca attctcaatt tattaaggag cgagtgcaga
     3421 gtcaagcttg cttgaactat gccgagagac gacagaaaat gtagcgaagc taaattattc
     3481 aattctcaat tatttttata aatttgcaca aaaaataata acagtgaaaa agattttata
     3541 tttaataatt atagtaaatt ctttgttttt acaaagttta ttttctcaag aattaagtca
     3601 aaatgctgta gttagcttaa ttactcaaga tgaaggtgat gagctttacg catattttgg
     3661 acatactgct ttaagaataa aagatgattc attaggcata gatttagctt ttaattatgg
     3721 tacttttgat ttctctacac ctaattttta cacaaaattt gcaaaaggaa ccttaaatta
     3781 ttgtttgagt gtagaaactt atgaaagtct tgttttttca tcaagtcata ataaaagaac
     3841 acttagagag caagttttaa atttaactta tgaagaaaag ctaaaaactt atcaactttt
     3901 agtaaattat tataattccg aagcaagata ttatagatac gatttctttt atgataattg
     3961 tgccacaaag attagagata ttattcaaga ggcaacaaat aatcgcattg acttctcaaa
     4021 atctaatttt aaaggacata gttacagaca gttaattatg ccatttttgg aaaagaatta
     4081 ttggatagac tttggaatta attttatttt aggtttagag gctgacaaaa aagttccacc
     4141 tgaacaatat atgtttttgc ctgtatatat tcacgatttt cttgaaggtt cagattttgc
     4201 gcaaaattca caagttgtat tagatgcttc ccctgcaaaa aaacaaggaa ttgactttgg
     4261 atatatttta ccttggttga tagtgcttgt ttggggaatt ttaatttatt tcaaaaaaac
     4321 aagaggattt ttcaaatatt tattattcac aatttttgga ttgcttggac tttttatatt
     4381 atgttttgga ttatattccg aacacactgc agtactatat aatttaaaca caatatggtc
     4441 tctcccagcc cttttaattt taatattttc atggtcgaaa aaatggttac attatattaa
     4501 acttggatat ttaattattc ttttactgca tttaatcaac tggttttggt tgccacaaca
     4561 aatgtcaact acttttttac cttggatgtg cttattgatt tttaccctat tggttgattt
     4621 gaattatttt aaatatttta agaaaagaaa agcaaccaaa aattaaaatc acgttttata
     4681 ataaactttt tttaattgaa tattaaattt atgaaattca aaaatttact tttagttatt
     4741 tctttaatat attttagcac aattatattt gctcaagaaa aatctgtgct atttattgga
     4801 aattcataca catactttct ttaatgagag tgtaccaact acttttaaaa aaatagccga
     4861 atctttgggc gaagaagtta ctacagagat gatagctcca ggtggataca gattttccaa
     4921 ccattgtaca aatcaagcta ctttagattt aattaatagt aggaattggg attatgttgt
     4981 attacaagaa caaagtcaac ttccgtcttt ccctccagct caagtagcta acgaagttta
     5041 tccttatgct gcatcgcttt gtaatactat tcgtcaaaac gacatttgct cacaaattat
     5101 tttctttatg acatggggac ataaaaacgg cgatgcttcc aattgtccat actatgaacc
     5161 tctttgttct tatgaaggaa tgcaatggcg tttacgtcaa tcttatgtag aaatggcaga
     5221 agataatcaa gcttgggttt cgcctgtagg aatggctttt gcacaaatac gacaaagtaa
     5281 tcccgaaata gaattatact catctgacaa ttctcaccca agcgcatatg gaacttacct
     5341 aggatagtta cactatttgc ttaacagcat acaaaggcaa ttgctattat gatacaattt
     5401 gtaaaccaag ccttattact ggcaagcctg aaattgaaaa taatttaact aaaattaata
     5461 tttaccctaa tccaagtttg gataaaataa tttttgttga aaatgctgaa aaccaagttt
     5521 gctctatctt tgatttggca ggtagaaaga tttgtcaaaa agaaataaaa gacaaaacat
     5581 tgaacttaac tgatttagca aatggaatgt atttaataga aataaattca gaaacattta
     5641 aaatacactt gaagtagatt tgagaagaag aaccaaaaca aaaagagact gtttttaaaa
     5701 gcagtctctt ttttgtttac cacaattttt tccatagtac gttttttggt aaacaaaacc
     5761 tgtaaaatag tttgtttaaa tcaaatattt attaaaaaca cttttaacaa ctccctaaca
     5821 atcaacacct tactaaagct tcctatttta ttaccagctc gctcggattt agcttcgctg
     5881 agctcgtcct tcggacttcg gttgtaatct gagtgtttga aatattctgt cgctgttgca
     5941 aatccgagcg aacggggttg tgtgaaaaat tggagtaagc agagtgagct tgcgaactct
     6001 gcgaagcaaa tatagacaac ccctgcacta aagaaattct ctctatcatt gtgaagtttt
     6061 taaaaactca ctactaaaaa ctaaaaacta aaaactcaac actaaaaact ctttcaactt
     6121 ttaactgacc tagtgagcca tataggattt gaacctatga cccctagcct gtcaagctag
     6181 tgctctaaac caactgagct aatgactcaa aattttatgc aaaactaatg tttttttagt
     6241 ttatctcaaa aaagttttgt ttttttctca tttttatttg ttttttaaaa aaaaatagat
     6301 tatatttaaa aagaaaatat tttagtatga aaaacaactt aaaatatgaa caaaaagatt
     6361 tattttcatt gagaaagttg ttttttaatt tattttacat aacacttacc cccacccccc
     6421 cccgcacctt ctaaaaacct tgccacacaa aggttgtaaa aaacttcatc atttcaaaag
     6481 cagacatgaa tacaaattat caaataaaaa agttgacaaa aattgttgta aaacaaataa
     6541 aagaaaaacc tggaacttaa cataggcgac ttgtctttaa agctttaaca agaccagtcg
     6601 ccgtaaaaat aatgctttca caagcaatgt ttaagctaca acagagtcta aaaatagcta
     6661 ttacagtaaa ctctgttgta aggaaaaata agagtaatag ctatattgtg aaatttatgc
     6721 ttgattttat aatgaatagt aaataagcaa aaaaaaataa aaattatgaa aaaaatattt
     6781 ttgattttaa tagcaatttt aatggcatcg tctttattta gtaaacaagt aacaattata
     6841 aaagagggag gtaaacttaa caaagatggc accgtaacgt acgatgatat taaacaacaa
     6901 tttaccaatg gcgagttaac atatcaacat tgtttaagaa gaggacatga aaaatgtcca
     6961 aaagttggaa ttgttactat tggaactggt gtatcaattg acaagtatat tgaattagca
     7021 gaagaggtta tagctaaaaa tcttgaaaaa ggaataacta cagggaaaat atcttatcca
     7081 tccgacaata ttatgattta ttacaaagag gcatcctttg ttttggacga aaacggaaaa
     7141 gaaacagata aaattgagta tcatatgaca attgagtatt aacacgaacg aaaagggttt
     7201 tatattaaat atattataaa atattatgct taaaaaattt acactacttt tacttacact
     7261 aattatcggc aagctaatgt tttgtcaaca ttttaatgta ataaagacaa ttgaactaga
     7321 gacattatat ggagaacaat tttatgctta ctatgataga atacaaagcg aaaacaaaac
     7381 tattattcca atatatccaa tatatgaaga tgattttgaa aatatgatat atgtcgtaat
     7441 tgacagtttg gacaatttct ctttattgca atggaaacgt gaagcagaca aagatggtta
     7501 taaagacact cctatagcag gaatattttt agataataac aagggtatat tgatatctga
     7561 atatttatat tattttcaaa taaaaggcaa acaaatgtta acaacaaaaa aaataaaacc
     7621 tccaaatcaa tctaaatatg gagaattcta tgagatatac ccgttaggcg ataattcttc
     7681 aaaatatgtt ttattagcaa atacttataa ttgtagttct agaaaaacta tttatgatat
     7741 tttcaggttt gcaaaattca acataaaaaa gaagaaaatt gaaaaaatca ttaatatcga
     7801 cgttggaaaa ggcatctttc tatcacattt tagtaatcat atgatatcaa cttcaaaaaa
     7861 taatattgct atttcacacc cttgcaaacc agaagtgtat ttgtttgata atgacctaaa
     7921 tcttcttgat acaattgtta tagattatcc aaatattttc aaaacaaaaa atctattaga
     7981 ttcaatttta acagatactt taattcttga aaatagatat atgacgatta atttattaca
     8041 gatattacat aacaacaaca ttatgaaata tccaagaata gataaagttg catttattga
     8101 cgaggattta ttatttattt ttgtaaaacc agatgctgtt gtagatacaa ataataattg
     8161 gcatagaaat cacttacatt atgtatattc tttatcaaac aaagattttt ttgaaacaaa
     8221 aggaaaaata agcaactttg atgctattaa ttcatcgcaa ccattaattt ttcacaataa
     8281 taaaactgtt gctgtttggg atagcgttga cacaaacaac ctttttaaat attacatttc
     8341 attttgcgaa atatcaatag actttaattc taaatatata gatacaatag acctatctat
     8401 tttaccacct tttttgcatt taaaaagcat agattctttg aataaaaacc aaaattatga
     8461 aaaatttaac tatattatgg tagcaaatta tgctgcatgt agccattgta aattttatga
     8521 caagtatcca aatttaatga ttattcatgt ttttgataaa aaagatgcat ataataggtc
     8581 aaccatgttg tcatataaaa ctaagtacaa taaaatgttt gataatccaa cagtctattt
     8641 ttgcgaagat tctatgatag atttaaataa aataaaaatt aatgcaattt ataaaactaa
     8701 gtaaaacttt tttaactctc aaaattaagg ctgaatctaa tataggttca gccttttttg
     8761 acaaatattt ttataaattt tatttttttt aatctacttt ttatccttat ctcaataaaa
     8821 tttataaatt tgaaaacaaa ctattaaaat ttgtgaagga ctaaactgca aaaaaactat
     8881 aaatttctaa ggtgcgagag cagagccaag ctgcttgaac tatgccgaga gacgacagaa
     8941 aatgtagcga agctaaatta ttcaattctt aattagttaa tcatcatctt catcagagtc
     9001 atctcctctc tttctttctt gcttttgcaa tagtttttct tgcttaatgg aatctctttg
     9061 gaatttcctt tctagttttg cttgccttaa agaatctcta gctatttctt tttcagattt
     9121 cataactttt tcgcaaacac atctaactcc tacccaatct tgtgatcctt tataataaaa
     9181 atccctatca actgttactt cataggcatt gtgtagccaa gctccgccct tagcaagtcc
     9241 tttagtgtcg gtcatttctg caacgtttcc aaaaatatga taacagccta ttaagttggt
     9301 ccaaaaagag ttaacttttg ctgttgtgtt tagattgatt gcattagttg cattttcacc
     9361 ctgatgtctt gggtaaatta aattataaat atttggatta taactctttt caatttgctt
     9421 taaagttttt ttagaaaaac ctaaagaagc tataacttcc cattcttgtt tacttggtaa
     9481 gcgatattta tatatttgtg gtatgccaac agtatcatct ttgagactgt atgctaactt
     9541 attttgtctt gcatacaaca tgtaattaac ttgaatactc cgccattcgc aataagcaac
     9601 agcttgttca taagttattc caacaatagg aaaattataa taagctggat gtttaaaata
     9661 tgattttgca acttgttcgt aaatagactt acgccatact gtggaatcgg gaaaacaagc
     9721 cttatactca tcagaatctt cgccatacat tctttttaac caaaagctat actcgttcca
     9781 atctgataca cttaactcac atatatcaac aaataaacta tcattaagtc ttgtggttcc
     9841 aggaggtgta actgtataag gattaaaagg taaaaacggt aaagtggaaa ttaaaaataa
     9901 acctaacaca caaaacgact tcatataaaa atcaaaaata tttttaaacg caacaaagat
     9961 ataattattc ttgttgattt aaaacttaaa atctgaaaaa accataatta tgcttgatta
    10021 taatattcaa aagttccgtc attaaataaa actatcactt tttcaaccgt tttaggctct
    10081 ttagagcttg aatttatctc tttgagcatt tctttgggtt catcaacaaa cttgtcttta
    10141 tcaagagttt ttgtagcttc ttcaaggttt ttaggttcat tatcaactgt ttttaaaggc
    10201 tcttgattgg aatatttatc ttcttcatag cttgcttgag aaaataaatc attctcaata
    10261 ggtttggacg aagataaatt agatgaaata gtaccctgac tttgtttgta catttctcct
    10321 ttacctaata ataaccattc tgaatttatt gctctaaatc tttctaataa tttttgaaca
    10381 aaatcaattg acggattatt tcgacctgac atgatatgag aaatactcga agcctgtact
    10441 ccaatttcgt ctgctaattt agaagctgtc attccttctt cctgtaaaat ttttgaaatt
    10501 ctattacgca ttttttacaa aattttaagt ttacaaatgt aataattata ttatttacat
    10561 atgtaaatat ttattaacaa ttatatgttt acatttgtaa atttacattt tgagcaattt
    10621 cgatgtttta atagaattaa actcattcaa aatcaatcaa attaacccta tttatcaagt
    10681 aataattatc atagtttgag cccctatttt aagcatttca ggctttttag agcattttag
    10741 gataaaatca atatctttaa gttaaatatt aactgaaata ttataaaata ctggttatca
    10801 agcttatata aagtctaaaa ttaagttttt ttgtccattt tagccattta tttacaagat
    10861 tatcaaactt agttaagtat ttacatgaaa tgattaacgt aattaactga atataagtat
    10921 attatattta taaaaatttg atgcttttta actcaaaata atttcacttt tgtaagttta
    10981 tttatattta caattgtaat tatttaatta aattattgca taaatctcta gttttacatt
    11041 tgtaaattag ataattttaa cttttatatg cttcacattt gtaaatttat tgaatttttg
    11101 taaattattt aaaattgtga atttaagcct aattttactc caagtataat aaatataaga
    11161 aaaaataaat cctgtcttct accctcttaa aattcgtttt aaggatattt tattttttta
    11221 cgagaaatta ttttttacaa aaaaataatt atctcctatt ttcgcacttt ttttttcaac
    11281 atccgctaac tctataataa tcaggtgtga tttattattt ttggcgtagt tatgttaaaa
    11341 atagaaatta catattttta attgtccgta aggacaatac atgactatca aaagtttgtc
    11401 cgtaggacaa catgtgatta accgtaggcg gtagcctacg gcagataaac cctcaaaagc
    11461 cttgcccgta gggcaacaca atacttttcc aatttaatct cgttcagtgt atcactttta
    11521 actaatttta ttgtgtcgcc cttgcgggcg aagaggtatg tgggtatatt ttcgccgtaa
    11581 gataaatctt acggttaatc acatgttgcc cttgcgggca aggcataatt gtttggcatg
    11641 gtgctgtaag gtttaaaaca ggttgtattt tattttttaa aattaattat ctcctatttt
    11701 tttgaattag taaattaaat atttaaaaaa atgtaaaatc ttatttttat aaaaactcaa
    11761 aatatttaat aattattttt tttgaaattt ctaagtcaat tttttttcaa taaaactgta
    11821 ttaagctccg aaaagaatta aatatcgcct ttaaaacgcc tttaaaattc aaaaatatta
    11881 cctaaactcg ttgagtaaga acgaatataa gctaaatata ttgtattttt tattttttaa
    11941 ttttatttaa aatattaata aaatcaactt cgaagttagg tgtaaagaca tttttcgata
    12001 aattcttttt aatttcctta aaactccaaa atttaacttc atctacttca tcatttttaa
    12061 caaaattaaa ttcttcgtgt tgagctagaa aaacaaaaac taattctttt tcaatttctg
    12121 actcccaaat atactttgta atatattgat attttaaatt tttaagacct aattcttcgt
    12181 aagtttctcg ttttaaagct gtctctaagt tctctccatg cgaaatatgt cctcctactg
    12241 ccgtgtccca ttttccgggt tgaataaact tattttcatt ccttttttgc aatagcacct
    12301 cgccttttga atttaaaata tgtacatgaa ttacaggatg cattctttta cttccatcat
    12361 gagcttggtc tcttgtcatc actcctatta ccttgccttc ggaattgagt tctgacaata
    12421 gttcttgttt tttgaatttt cgtctggcga taatattttt cacaagctca acaacaaaaa
    12481 agactgcaaa cagaatgtaa aacagtccac cactaataaa agcccaagct tcttttgaca
    12541 tgaaaaaagc agaataaaac actaaaacag tgtgtgccgc aaacaaccaa aacattgttt
    12601 tgatattcct tcggaatagt ttttcttgtt cgttactaat ttccaagcct ttaaagtagt
    12661 gagccgacat tgccaacaac aaatttttgg gcgaaaatgc agaaactcct aaaaatgcaa
    12721 taagaattaa gccaattatt cctggtttta gtttgaaaaa tatatcgttt tctaaaatta
    12781 ttgacaccac tcccatcaac aatattaaaa tagtgtcaaa tagaacgaat ttatcaagtt
    12841 ttttctcttt tatgtatatt aacaataact gcactaaacc tacgccaata gccacatata
    12901 gtcctattgt agtaccccaa atttcatcgg ctaaaataaa taccaatagt ggcagtaagc
    12961 cgggaagaat tatttttaga atttcaattt ttttcattta ttaaataacg atgtccataa
    13021 atgtccaact gctgtcctaa gtttataaag ttaattttat aacaaaatgt gtcaacctct
    13081 ttggacgcgt cagccacttt taacttttaa cacttcaact acgttcagtg catcgctttt
    13141 aacttttaac taaattgtcg gacaaccacc ttgaattaca aaatttggaa tcactctatg
    13201 aaatcttaat ccattgtaaa atcctgattc tgctaatttt ataaaatttg caactgtatt
    13261 aggtgcatca tcatgataaa attcaacagt catattgcct ttatctgttt taattattgc
    13321 ttttttcata tactatttta tttgttttaa atcctaaatc aatgaatttt tatattcgta
    13381 aatcttactc gtttttccaa tttccaaatt taaactcgca atcacaaaat gttttatcaa
    13441 ttcttatata agtactttct aatttattat gaatccactt ttcaattatt ttttgtcctt
    13501 tttgttgtgt tgcatattct tgaatttcaa gataatcgtc tgttaaatta gctttatgtt
    13561 cttctacttt ttggtcgagt ctaacaattt ttataagctt tgctcctttg cttgagctag
    13621 ataaaaatgg ctttgtgtat tctcctactt ttaaaccagc aatatttcta acagtgtttg
    13681 ggtcaacaaa ctcattagct aattttgtag ttccataata tggattaata actttaccat
    13741 tgttaaattt tgtttctcca tcgctatatt ttgttacagc ttcgctaaaa cttatagagt
    13801 ctttttgcag caattcgtaa acttcaagtg ctttttcttc agtaagacta atttgttcaa
    13861 tactaacttc aggtgtcaat aaaatatgtc taaaattcat caaatttcct tttttctcaa
    13921 taagttgaat tatatgatat ccatattcag tttctaccac tctcgacact tcgattgggc
    13981 ttgttaaatt aaatgcaaca gaagcaaatt cgggaactaa atctgttcga ctaacaaaac
    14041 cgagttcacc acctctagtt gcagagccag gatcttctga atataaaatt gctaaagttt
    14101 gaaaactttc gccgtttaaa attctgtctc tcaagtcgtt tagcttttga atagttgcat
    14161 ctttttcttc tttaccaatt tctggtgcaa acacaatttc tgaaagttcg aaaaatgcag
    14221 aaaccatagg aatactatct tctggaattt tttcaaaaaa ctctttaact tcacttggag
    14281 taattttaat atctgacact aagcctgctt gtactctttg agttaaaagc tgttcgtgaa
    14341 ttatagactt aaagtctttt tttatttcgc ttatagattt tccataaaat tcctccattt
    14401 ttttctcact tcctaattga tttacaaaaa cggaaatacg tctatctaac tctgattcaa
    14461 tttctttgtc tgtaatttca acactatctt gaagtgcttg caagtacaat aatttttgaa
    14521 acataagttc ttctaaaata tcgcatctta aatctccgtt taaatcataa gtaccttgag
    14581 ctctaagctg aatatattga ttttctacat ctgattttaa aacaatctca tttccaacta
    14641 ctgcaacaac ttggtcgata acctcttgcg aaaaatgagt ttttgaaatc aagaaaacca
    14701 agattaataa tattatattt ttcactgttt aaattttaat taattactaa tttgccacta
    14761 tttatagcat ttgtttttaa ctcgtttttt acttgttgat atttactatc ttttctgttt
    14821 ttcataatta cttgataaat aagcgtttca gctaattcta aaggcatagt atcgccttca
    14881 gtaataattt catctatttt tactaaataa cgcatatcat cataaacata gtttacaaaa
    14941 ttttgatttt gagaaaaacc atttatatct ccttgataat gaattatttc taaaaaatca
    15001 tctaattcaa tccaatcttt taaaaaataa accttacgtc ctgtacctat gcaataatcg
    15061 ataagttctt tttcgtcttc taacgaactt tttctaaccg tttcgtattc ataataatga
    15121 gatgcttttg ggtctaaaat tgtcatgtaa tgagctttaa cataagtgtt tgacaattta
    15181 taattatcca aatatttatt ataatattct tctatttcat cacggctaac tttatcattt
    15241 attgaattat aaacatgatt attcagatac tcatttacat ataaattttt gcgataatct
    15301 tctatttttc gacaaatttt tgaactatct gtaattttta ttttagcttc atcaaaaaac
    15361 aatttttgct ctaaccatgc atcaattaga gagttaacat agtttattga atctgtttga
    15421 gaattaaatt ttggcaactt tttaataata tcttcttcta gcaaatattc attgccatat
    15481 tttacaataa ttttaccttg aggttctttg tttttattag cacaagaact caataaaatt
    15541 aatattgcaa tatttataag aagtgcaaaa aatctcattt taatttgcta atgtttttaa
    15601 aacatcttta tgaaccttta tattgtattt gcttcttaaa gtttttatcc attctttttc
    15661 taaatagttt tgataatcag ctgtgataat acctttggct tcatttaatg ttttaacttc
    15721 tggtccatgc acttctatta aagttattgt attattttcg ccttgtgaaa ctaatctaaa
    15781 accgtctgtt tctgaaatat taaactgttt tattttagaa tctactaatg gttttacacc
    15841 tttttcagca aatgcacttt cgctaagaac aatagcagta ctatcttttt tatttatttt
    15901 tgctaagata aattcttcgc ttttcttttt gttaaatagt ttcattgctg aatttaaaac
    15961 tttagcatct ttacattcat aaactctaac atcataacga taatcccaca tataggaatt
    16021 tttattttct tggtagaatt tttcaagtcc tacagtatct tgaatagctt tggaccaaac
    16081 aaatctatct gttaattcaa aaagtaaaat tccatcgtga tattctttta ttaaataacg
    16141 aaaatctgga tatttatttt ccaaaatatc ttcttcatac ataagaatca tttttgagac
    16201 aaagttgtta taagcattgt caacaaacat tttaacatct tgtggacttt gtttgcggtt
    16261 atatttcatt aaataatcca caaaatcttt ttgtgttttt gtttgattag caaatgttaa
    16321 aattggttta tctaatttta gattttcatc taccgcccat gttccgttaa agattgaatc
    16381 tgttactaag gtataaaata atttaagatt ttcgtctata gtttttgcat tatattcatt
    16441 ttttagagta tttactatag catctctact ttttgatgtt cttgctgtgt ttgagataag
    16501 tctttttaaa tcattttttg cttcatcaaa aggcttaatt tcctctttat caacaagttt
    16561 aatcacatgg taaccataat ttgttttaag tactggtgca atatcaccaa tattttctag
    16621 tttaaaaaca gcttcttcaa attctttaat cattcttcca cctacactaa cccagcctat
    16681 cattcctccg ttagttgcag ttcttctatc ttcgctatat tcatctgcta attgtgcaaa
    16741 atcttcgcct gctatagctt tttgataaac ttcattaatt ttat
//
LOCUS       DAOPNX010000058        16147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_4300_length_16147_cov_5.939784, whole genome shotgun sequence.
ACCESSION   DAOPNX010000058 DAOPNX010000000
VERSION     DAOPNX010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16147
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4300_length_16147_cov_5.939784"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="PKN53_07435"
     CDS             <1..1103
                     /locus_tag="PKN53_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOD88816.1"
                     /translation="KFVKEYFNFSKRETNGILFLAIIMVIIIGLRVYFNNYAKPKKLD
                     FTEYETEIDDFLAYSLPDLENGERYDFDPNIVSREELLFMGLSRKTVDGIISYQERGY
                     KFWKPEDLKKVYGITDEEYEAIKDYVKIESSKSYNYKNSYKNYYKKFDKSSEIMLFNF
                     DPNTASYSDLKNLGFSNNQANNIINYRNKGGKFYTANDLLKLYSIDSVCFNRVKDYIK
                     ITDNVKEDLVKSENREVKVYINSADALEFQKLRGIGEVFSKRIVNYREKLGGFVSKEQ
                     IKEVYGISNELYENIEANLILDESEIKKININKADYAELISHPYIDKEITVVILNYRE
                     FKGEITSFDELLSQKALTKKSLEKIQPYVSLK"
     gene            complement(1156..2271)
                     /locus_tag="PKN53_07440"
     CDS             complement(1156..2271)
                     /locus_tag="PKN53_07440"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOD88817.1"
                     /translation="MKNIYLISVLTILVAGFLNTAVYAGNEQRAASNGASELQINPWA
                     RSSGWGDANVACVTGVEAMYQNIAGLAMTRRTELVFTNTQWLVGSGVVINNGGFATKV
                     GETGVIGLSVMNINWGDIEITTDALPEGGVGTYSPTYTTIGLSYAREFSNSIYGGVTV
                     KLLNESIFNLNASGFAIDAGIQYVTGFGRDKARNRNRDNLKFGITMKNVGSTMKFTGD
                     GMSFMGFSQNGTNMTVENRSQEFELPALIKIGFSYSIRLAAKVDEFDNTVKSDHNLVI
                     AANFTANSYEKDQFHAGLEYGFKDLFFLRGGYMYQKGMLDPNPENRATVFTGPTAGLT
                     LQVPINKENKSVLAIDYSYRFTNPFRGVHTFGARVSL"
     gene            complement(2300..6418)
                     /locus_tag="PKN53_07445"
     CDS             complement(2300..6418)
                     /locus_tag="PKN53_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88818.1"
                     /translation="MKNLRKILILAVLIAISNIMLADKWKGEPRPPHKIDRAVAGCEP
                     AKASTEIAINNVRTLIHTGGDMWWDIFGTRKAAYEVPVGSGCHALFAGAIWVGGKDAN
                     GQLKLAAQRFRQDGIDYWPGPLTTDGLAYVSPEICTKYDRHFVITKEMVREFREWWKC
                     TQNPECDESADFPNYQIPDIILNWPAHGPEGGYAHNLAPWWDVDGDGVYNPLNGDFPY
                     YEFPGEGITNDPDCHRPRGQASRLFGDHTLWWVYNDKGNIHTETGGAAIGMEFRAQYF
                     AFATNDELNDMTFANYNIINRSTYILTDTYFGVWTDADLGYPNDDYTGCDVQRGLGYM
                     YNGDDFDESASGHNGYGSQPPAIGIDFFEGPYQDPDGKDNSTSYDTINGVKILNCNKG
                     DIMNGNINGLNFQDGEVDNERWGMRRFLYFNNNTGGNPYTQDPHTAQEHYNYLTGYWL
                     DNSPLCYGGTGHKSGGGDPNKPTNFMFPGNPTTDPCGWGLGGVPQNEPWSEETENNPP
                     DDRRFVQSAGPFTLKPGAINDITIGAVYSRAASGGPWASVEAVRRADDKAQLLFENCF
                     RVLDGPDAPELKIIELDKKLIFHIYNRPGSNNYLEAYVEKDPAIVCHPDIVPCDEYYR
                     FQGYQVFQYRDRSVSISDRYNNDKVRTIFQCDIKDDVSQIVNYTWSDELRANIPVEEV
                     NGQNVGIKHTFVIDKDVFATGDPKLINNREYYFSALAYGYNNSMLYNQSIQETFNGQK
                     KPYLAGRNNIKKYVAIPHMNDADNSGTIMQSEYGEGLQITMYEGHGNANNIIELTDET
                     IDEIMSGYPWKVSKRVYKNGKGPINVKVIDPLNVPEGTYYLKFNQDALTHAFGFIGTT
                     KSELSPSNFEPFGYTISYGDKVINSDVDVKYGIGDEQLLMDLGISVTISQDNFALLED
                     KNTYNNGFLDAELIFEDETKPWLVFLPDGDNFDAFNWIRAGSYTNPVQFGDCIDKSYN
                     DKVGYDDDKHFGKILGGTWAPYFITHSGRYGVSLNGSHSNINIGRTEPLSSVDIVITS
                     DKSKWTRACVVEMHENEWREDDCGFYQEVLPKVNYLSVGNALKFTLRKSPSVDKEGKP
                     LNDGTYGLSWFPGYAIDHRTGSRLNIVFGEDSWLTGDNGADMIWNPTFGINSERGPIF
                     GGKHYIYILGTNQQANAANNCPAYDSCQWFYNKLIEFEQTNNSTILNRAWASAMWVAI
                     PLANPNFDFLATDVRIKLRVGTPYHKGLNNFAVDNPVNDNYPYFSFTTKDIQTIKNHN
                     ETAVNALDLIRVVPNPYYGSSYYEHNQLDNYVRITNLPQRCVVSIYNSAGVLVRKLEK
                     DNTDPFLEWDMKNTYGISIASGVYIVHVDAPGIGEKVVKWFGALRPIDLNNF"
     gene            complement(6449..10072)
                     /locus_tag="PKN53_07450"
     CDS             complement(6449..10072)
                     /locus_tag="PKN53_07450"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOD88819.1"
                     /translation="MIRKLLFILVALITSAGVYAQSAGTLSGKVTDAATGEPIPFANV
                     TIEENGNIVTGGMTDFDGNFSIKPIPAGRFTVSASYIGYATLQYTNVQIIAGKIVYQN
                     FDLNPAAEILGEVEIKEYKVPLISKDQTQSGGTVTSEDFAKMPGRSAAAVATTVAGVY
                     TEDGEVGSIRGARSEGTVYYVDGVKVRGSSAVPKSAIEQVTVITGGLPAQYGDVTGGV
                     ISATTKGPSSHFYGGGEVVTSQYLDPYGYNLAAIMVSGPIWSVTEKGESNRKRTIAGF
                     LLAAEGRIVKDGGPSAVGEWRANADVLEKIRTNPLRLIESQSGNIIYQNSDYLHADAF
                     QNYKSRANAGGKGANVAAKIDISPIRDLNFTIGGTFDIGAGRLYSYGNQLFNSHNNGN
                     YISDAWNTYARITQKFRTEDTAVIKNPYYTIQFDYSRSSSNYFDSKFKDNYFEYGHIG
                     NFTTVPEKYYIWGIDSVTGLEGNLFETYFYRITDFTTNELNKDLARYTEQYFDYFSDY
                     YYKDVDHLLSYGGLMNGNGAPSIYGMYASPGNPYASFEKSEGQQFRVSASGTADIKSH
                     EFSLGFEFEQRKDSYYAVAPFGLWTLARRLTNMHILEKDISKPIIYYDDQGNFLDTID
                     YPRLYNAQGQSRFDKYFRESLGMEINDLTWIDVDSYDPSTFDISWFSADELFNQGSPV
                     VAYYGYDYQGNKLHSKPTFDDFFNKTDEKGYKTRPIAPFEPSYVAGYIQDKFAFKDLI
                     FNLGVRVDRYDANQMVLKDQYLLHEAYKLGDTDELGLLANSTHPENIPNGATVYVNDL
                     SNPTAINGYRVGNVWYNSSGIEIENPNLIASPNGIAPYLINPKEELNTKAFEDYKPQI
                     VVMPRISFSFPISDEALFFAHYDILSKRPSYSNRLNPIEYLYIRQMNADLLNNPNLKT
                     EKTIDYELGFQQKLGNTSALKLSAFYREMRDMQQAIAVYGAYPATYYTYGNIDFGTVK
                     GFGVSYDMRRTGNVTLRASYTLQFANGTGSSYSSGAAIVMSDKPNLRTTLPLDFDQRH
                     NLAVTVDYRYGGKVSGLPYNGPKIAGKSILANTGVNFIINSGSGSPYTKRNRPNSGTV
                     VGSLNGSRKPWRTTINMRLDRDFRIKIGNKKESGNKEKYSDLNVYLEISNLLNAKNVL
                     GVYSYTGNPDDDGYLNYADYQTSINTQNDVEAFRNYYSMYINSPYNYSTPRTIRLGVQ
                     FGF"
     gene            complement(10223..10573)
                     /locus_tag="PKN53_07455"
     CDS             complement(10223..10573)
                     /locus_tag="PKN53_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88820.1"
                     /translation="MAKLRKIIQILLIINFILCSSTSFSQNIRSIDNEKHMKSLRQKR
                     YRPSPEERLVMKIEKNKAKKDAKKKKRDARLHKKAVKKHNKLINGGGKDVVDGKRTYK
                     RMKKSKKSAKYTRE"
     gene            10589..11029
                     /locus_tag="PKN53_07460"
     CDS             10589..11029
                     /locus_tag="PKN53_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88821.1"
                     /translation="MKVYIAKIIIFFTCVGFFTIIPACENEERIPYAYVDFILYLDMP
                     QYFEVRTPGNYLYLTGGVKGIILYCEYADSYRAYERNCPYKPSDPNAILEVDSTGVFL
                     VCRHCESKFLIMDGSVVNGPAKYPVLQYATYLEGGNRLSVFNAF"
     gene            complement(11099..12514)
                     /gene="ahcY"
                     /locus_tag="PKN53_07465"
     CDS             complement(11099..12514)
                     /gene="ahcY"
                     /locus_tag="PKN53_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOD88822.1"
                     /translation="MNELISSLPYKIKDINLADWGRKEIEIAEKEMPGLMSVRKKYAG
                     SKPLKGARITGSLHMTIQTAVLIETLKELGADVRWASCNIFSTQDHAAAAIAKAGVPV
                     FAWKGETLEEYWWCTLQALSFPGGLGPNLIVDDGGDATLMVHKGYYAEKNASTLDKKP
                     GSDEERIIFETLKKVLAVDNSKWTRAVKDLKGISEETTTGVHRLYQMMEKGELLVPTI
                     NVNDSVTKSKFDNLYGCHESLADGIKRATDVMIAGKVVVVLGFGDVGKGCARSMKGYG
                     ARVIVTEIDPICALQAAMEGFEVARIEDTFNEGNIYVTTTGNRDVITLEHMKQMKDQA
                     IVCNIGHFDNEIQVDNLNNYKGAKRTTIKPQVDKYTFENGNSIFLLAEGRLVNLGCAT
                     GHPSFVMSNSFSNQTLAQLDLWQNKYEVGVYRLPKHLDEEVARLHLEQIGVRLTKLTK
                     EQADYIGVPVEGPFKPEHYRY"
     gene            complement(12585..13502)
                     /gene="ribF"
                     /locus_tag="PKN53_07470"
     CDS             complement(12585..13502)
                     /gene="ribF"
                     /locus_tag="PKN53_07470"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HOD88823.1"
                     /translation="MKVFTNINKNIPKNCVVSIGFFDGVHKGHVYLLKQLMNEANSKN
                     QEELVITLWPHPAKYFGKNISLLNTLNEKIVLLEGLGIKNVLILDFNEELASLSANMF
                     VENILKEKLSCSSIIMGYNNHFGNKNSTSKPESEFAIPVKRLQKFEFEEFTNINSSQI
                     RECITQGDLEKANKMLGYNYKLSGNIISGYQIGRKLGFPTANLEVSDQDKIQPPNGVY
                     VAKAYFDKKSYPAMLSKGTRPSFDGKELSLEFHIPNYEGDLYGKQVTIEFIKKMRDEI
                     KYENIDDLITQIKKDQEETLAYFSKNLGF"
     gene            13528..14733
                     /locus_tag="PKN53_07475"
     CDS             13528..14733
                     /locus_tag="PKN53_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOD88824.1"
                     /translation="MKINYTYLSLNKSRTLILMVLILLSFVFLLSIISCKNNHKQVEI
                     FGIIENAENKEIYLEMLLPGQIKPLVQTEINEKGEFKFELDSLQTCFYRLKIDERNTI
                     YLRLNPNDEIEINASYPNIQANYSIDGSEESKNLKELNMYLMKSTKRLNEINDQIVAA
                     YKIPNYDVDSLIEVLNVEARAMYKNDKEFLINFIKKHNESVIIYFALYQYVSISPILI
                     IDQDIEIFQFVLEELKKHNPEFEQIALLESEISKELLRQQQFNREYGKLEIGKPVVDF
                     DLPDINGNRVSLSKFKNQKIILAFWSSWNKVSLNAVLGLSKKLENQDVVLILIALDNA
                     KDKWETAVKNNFLEKHINLSDLKAWESPVAKIYAVKSIPSFLLIDENQNLVAISDDYN
                     EIIDNLNKN"
     gene            14793..15548
                     /locus_tag="PKN53_07480"
     CDS             14793..15548
                     /locus_tag="PKN53_07480"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOD88825.1"
                     /translation="MKEKNKIYFVSDSHFGIDYDKYSSEFREELMLSWLDEIKSDALE
                     LHLCGDMFDFWFEYKYLVPRGFTRLFAKLRELSDLGVKLYYYLGNHDMWTFGYIEETI
                     GAKVFKGVNLKEINGKIFYIGHGDGLGPYDKKYNFLKKIFSSKFMQFMFRLVHPSISF
                     RIATNWSSSSRKKHKYPKQVDFEKEWLVRYARTVLENQKIDYFIFGHRHIPFQYNLNA
                     NTLFTNLGDWLFNFTYAVYDGENLLLKTYEKAE"
     gene            15661..>16147
                     /locus_tag="PKN53_07485"
     CDS             15661..>16147
                     /locus_tag="PKN53_07485"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOD88826.1"
                     /translation="MKKIFYLVLVVFGFSCFANAQSTKVLIETSMGDMTVVLYDDTPE
                     HRDNFIKLANEGFYEDLLFHRVINGFMIQGGDPDSKGAAPNKPLGNGGPGYTLPAEFN
                     PKYIHKKGALAAARTGDHVNPERRSSGSQFYIVQGAKYTDMQMDSMEKQFRRKFTDKD
                     RD"
BASE COUNT         5287 a         2650 c         2474 g         5736 t
ORIGIN      
        1 caaaatttgt aaaagaatat tttaatttct caaaacgaga aactaatgga attttatttt
       61 tagctatcat tatggttata atcataggat tacgtgtata ttttaataat tacgcaaaac
      121 ctaaaaaact tgactttacc gaatatgaaa cagaaataga tgatttttta gcctattctt
      181 tacctgattt agaaaatgga gaaagatacg actttgaccc aaatattgtt tcaagagaag
      241 aacttctttt tatgggttta tcgcgtaaaa cagttgatgg aataatctca tatcaagaaa
      301 ggggttataa gttttggaag cctgaagatt tgaaaaaagt ttatggtatt acagatgaag
      361 agtatgaagc tattaaagat tacgttaaga tagagtcttc taaatcatat aattataaaa
      421 actcttacaa aaattattac aaaaaatttg ataagtcttc tgaaattatg ttgtttaatt
      481 tcgacccaaa tactgcaagt tattcagatt tgaaaaattt agggttttca aataatcaag
      541 ccaacaacat tattaattat cgtaacaagg gcggtaagtt ttatactgca aatgatttgt
      601 taaaattgta ctctattgat agtgtgtgtt ttaatagagt aaaagactat atcaaaataa
      661 ctgataatgt taaggaagat ttagttaaat ctgaaaatag agaagttaaa gtttatatta
      721 attctgcaga tgctcttgaa tttcaaaaat taaggggaat tggagaggtt ttttcaaaac
      781 gtatagtaaa ttatcgtgaa aaattaggtg gttttgtttc caaagaacag attaaagaag
      841 tttatggaat ttcaaatgaa ttgtatgaaa acattgaagc taatttgata ttagatgaaa
      901 gtgaaataaa gaaaataaat attaacaaag ctgattatgc tgagttaatt tcacatccat
      961 atatagataa agaaattacc gtagttattt taaattatcg tgaatttaaa ggagaaatta
     1021 caagttttga tgagttactg tcgcaaaagg cgttgacaaa aaaatcttta gagaaaattc
     1081 aaccttatgt tagtctaaaa taaaaaaaaa ccgacttttg tcggtttttt tattaactat
     1141 aaatttttta gatttttaca aacttactct agctccaaag gtatgaacac ctcggaaagg
     1201 atttgtaaat ctgtaagagt aatcaatagc aagtacagat ttattttctt tatttattgg
     1261 aacttgtagt gttaaaccag cagttggacc agtaaataca gtagctctgt tttcaggatt
     1321 aggatctagc attccttttt gatacatgta accacctcta aggaagaata aatctttaaa
     1381 tccatattct aaaccagcat ggaattggtc tttttcatat gagtttgcag taaagtttgc
     1441 tgcaataact aagttgtggt ctgatttaac tgtattgtca aattcatcaa cttttgctgc
     1501 taatcttatt gaataagaga atccaatttt aattaatgct ggtaattcaa actcttgact
     1561 tctattttca acagtcatat ttgtaccatt ttgagaaaat cccataaaac tcataccgtc
     1621 tccggtaaat ttcattgtac ttcccacatt tttcatggta ataccaaatt taagattatc
     1681 tctatttctg tttctagctt tatctctacc aaagccggta acatattgta ttcctgcgtc
     1741 aattgcaaag ccagaggcgt ttaagttaaa aatactttcg ttgagcaatt ttactgtaac
     1801 accaccatat atactgttag agaactcgcg agcatatgat aatcctattg ttgtataagt
     1861 tggggaataa gtaccaacac caccttctgg aagtgcatct gtggttattt caatatcacc
     1921 ccaatttatg ttcattacac tcagaccaat aacaccagtt tctcctactt tcgtagcaaa
     1981 accaccatta ttaattacta cacccgaacc cactaaccat tgtgtattag tgaaaactaa
     2041 ttctgttcta cgagtcatag ctagacctgc tatattttgg tacattgctt caacgcctgt
     2101 aacacaagct acgttagcgt ctccccaacc agaactacgc gcccatggat ttatttgtaa
     2161 ttccgaagct ccgttagatg ctgcacgttg ttcgtttcct gcatatacag cagtatttaa
     2221 aaaacctgct actaaaattg tcagaactga tattaaatat atatttttca tattacttta
     2281 tttattatat tttaatattt tagaaattat ttaaatctat tggacgtaaa gcaccaaacc
     2341 atttaacaac tttttctcca attcctggtg catcaacatg tacaatatat actccgctag
     2401 ctatagaaat accataagta tttttcatat cccattctag gaatggatct gtattgtctt
     2461 tctctaattt tcttacaagt acgccagcag agttgtaaat agaaactaca caacgttgtg
     2521 gtaaatttgt aattcttacg tagttatcta attggttatg ttcgtaatat gacgagccat
     2581 aatatggatt aggtactact cgaattaagt caagagcatt tacagctgtt tcgttgtgat
     2641 ttttaatagt ttgaatatct tttgttgtaa atgaaaagta tggatagtta tcatttacag
     2701 gattgtcaac agcaaaattg tttaaacctt tgtgataagg agtaccaact ctaagtttaa
     2761 ttctaacatc tgttgctaag aaatcaaaat taggattagc taaaggtata gctacccaca
     2821 tcgccgaagc ccatgctcta tttaaaatag tagagttatt tgtttgttca aattctatca
     2881 acttgttgta aaaccattga caagaatcgt atgcagggca gttgttagct gcatttgctt
     2941 gttgatttgt tcctaaaata tagatatagt gttttccacc aaatattgga cctctttcag
     3001 aattaattcc aaatgttgga ttccatatca tatctgcgcc gttatcgcca gttaaccaag
     3061 aatcttctcc aaatacaata ttcaaacggc tacctgttct atggtcaatt gcataaccag
     3121 ggaaccagct taaaccataa gtaccatcat ttagaggttt tccttcctta tcaacagaag
     3181 gagattttct tagtgtaaat tttaaagcat tccctacaga taagtagttt actttaggta
     3241 atacttcttg atagaatcca caatcatctt ctctccattc attttcatgc atttctacaa
     3301 cacatgctct tgtccatttt gatttatcac tagttataac tatatcgacg ctggataatg
     3361 gttctgttct tccaatatta atgtttgaat gagagccatt taatgataca ccatatcgtc
     3421 cagaatgagt aatgaaatat ggagcccaag taccgccaag aatttttcca aaatgtttgt
     3481 catcatcata acctacttta tcgttataag atttatcaat acaatcgcca aattgtactg
     3541 ggtttgtata acttcctgct ctaatccagt taaatgcgtc aaaattatct ccatcaggta
     3601 agaaaactaa ccatggttta gtttcatctt cgaagattaa ttcagcatct aagaatccat
     3661 tattataagt atttttgtct tctaagagag caaagttgtc ttgagagatt gtaacagaaa
     3721 tacccaagtc cattagtaat tgttcatcgc caataccgta ttttacatct acatcagaat
     3781 taataacttt atcgccataa gaaattgtgt atccaaatgg ttcaaaatta cttggagata
     3841 attcagattt tgttgttcca ataaatccaa aagcgtgggt aagggcatct tggttaaatt
     3901 tcaaataata agtgccttca ggtacgttaa gaggatcaat aacttttacg ttgataggac
     3961 ctttaccgtt tttgtaaact ctttttgaaa ctttccatgg ataaccactc ataatttcat
     4021 caatagtttc atctgttaat tcaattatat tatttgcgtt tccatgtcct tcgtacattg
     4081 ttatttgtaa accttcgcca tattcacttt gcataattgt tccactatta tcagcatcgt
     4141 tcatatgtgg tattgcaaca tactttttaa tattatttct acctgctaaa tatggtttct
     4201 tttgtccatt aaatgtttct tgaattgatt ggttataaag catagaattg ttataaccat
     4261 aagcaagagc actaaaataa tattctctat tatttattaa tttagggtca ccagtagcaa
     4321 acacgtcttt atcaataaca aatgtatgtt taatacctac attttgaccg ttaacttctt
     4381 ctactggaat gtttgctctt aattcgtcag accaagtata gtttacaatt tgagacacat
     4441 cgtctttaat atcgcattgg aatattgttc taactttgtc attattatat ctatcagaaa
     4501 tacttacaga tctatctcta tattggaaaa cttgataacc ttggaaacga taatattcat
     4561 cacaaggaac tatatcaggg tgacaaacaa tagctggatc cttttctacg taagcttcta
     4621 ggtagttatt gctaccaggt ctattataaa tgtggaaaat aagttttttg tctaattcaa
     4681 taatttttaa ttcaggagca tctggtccgt ctaatacgcg gaaacagttt tcaaacaata
     4741 attgagcttt atcgtcagct ctacgaacag cttctactga tgcccaagga cctccagaag
     4801 cagctctaga ataaacagct ccgatagtaa tatcattaat agctcctggt tttagagtaa
     4861 aaggaccagc agattgaaca aaacgtctat catctggagg attgttttct gtttcttcag
     4921 accaaggttc gttttgtggc acaccaccta atccccaacc acatgggtct gttgttggat
     4981 taccagggaa cataaagttt gttggtttat ttggatcacc accacctgat ttatgtccag
     5041 ttccgccata gcaaagaggt gagttgtcta accaatatcc agttaggtaa ttatagtgtt
     5101 cttgtgctgt atgagggtct tgtgtatatg gattacctcc agtattatta ttaaaatata
     5161 agaatcttct cataccccat ctttcattat ctacttcacc atcttggaaa tttaaaccat
     5221 tgatattacc attcataata tcacctttat tacaatttaa tattttcaca ccattaattg
     5281 tatcataaga agttgaatta tctttaccgt caggatcttg ataaggccct tcaaagaaat
     5341 caattccaat tgctggtggt tgagaaccgt atccattgtg tccactagca gattcatcaa
     5401 aatcatcacc attatacata tatcctaaac cacgttgaac atcacaacct gtatagtcat
     5461 cattaggata acctaaatcg gcatctgtcc aaactccaaa gtaagtatct gttaaaatat
     5521 atgttgaacg gtttataata ttataattag cgaatgtcat gtcattaagc tcgtcatttg
     5581 ttgcaaaagc aaaatattga gctctaaatt ccataccaat tgctgcacca ccagtttcgg
     5641 tatgaatatt tcctttgtca ttataaaccc accataaagt atggtcacca aataacctgg
     5701 acgcttgtcc acgtggacga tgacagtcag ggtcgtttgt aattccttcg cctgggaatt
     5761 cataatatgg aaaatcacca tttaacggat tgtaaacacc gtcaccatca acgtcccacc
     5821 atggtgctag gttatgtgca taacctccct caggaccatg tgcgggccaa tttaatatga
     5881 tatcaggaat ttgataattt gggaaatctg cactttcatc acattctgga ttttgtgtac
     5941 atttccacca ttctctaaat tctctaacca tttcttttgt aattacaaaa tgtctatcat
     6001 attttgtaca aatttctgga ctaacatatg ctaaaccatc tgttgttaaa ggtccaggcc
     6061 aataatcaat accatcttgg cggaatcgct gagccgcaag ttttaattgt ccgttggcat
     6121 ctttacctcc aacccaaatc gcaccagcaa ataatgcatg acaaccgcta cctacaggaa
     6181 cttcataagc agcttttcta gttccaaaaa tatcccacca catgtcaccg ccagtatgga
     6241 tcaatgttct aacgttatta atagctattt cagtagatgc tttggctggt tcacaacctg
     6301 caacagctct atctatttta tgaggaggtc ttggttcacc tttccattta tctgctagca
     6361 ttatattaga aattgctatc agaacagcta aaattagtat ttttctaaga tttttcatat
     6421 ctattattta taatctaaaa tttaataatt aaaaaccaaa ttgaacacct aaacgtatag
     6481 ttctaggcgt actgtaattg taaggagaat ttatatacat tgagtaataa tttctaaatg
     6541 cttcaacatc attttgtgtg ttgatagatg tttgatagtc tgcatagttc aagtatccat
     6601 catcatcagg gttaccagtg taagaataaa ctccaagaac atttttagca tttagcaagt
     6661 tagatatttc taaatatacg tttaagtcgc tatatttttc tttatttcca ctttcttttt
     6721 tgttacctat ttttattcta aagtctctgt ctaaacgcat atttattgta gtacgccaag
     6781 gtttacgaga accatttaat gaacctacaa cagtaccact gtttggtcta tttctttttg
     6841 tataaggaga accagaacca ctgtttataa taaagttaac tccagtattc gctaaaatac
     6901 ttttgccagc aatttttggt ccgttataag gaagacctga aactttacct ccatatctat
     6961 agtcaacagt tactgctaaa ttgtgtcttt ggtcaaaatc taaaggtaaa gtagttctta
     7021 gatttggttt atcagacatt acgattgcag caccagaaga gtaacttgaa ccagttccat
     7081 ttgcgaactg taatgtatat gaagctctca aagttacgtt tcctgtacgt ctcatatcat
     7141 aactaacacc aaatccttta acagttccaa agtcaatgtt accatatgtg tagtaagttg
     7201 caggataagc accataaacc gcaatagctt gttgcatatc acgcatttca cggtagaatg
     7261 cagaaagttt caaagcagat gtatttccta atttttgttg gaaccctaat tcatagtcaa
     7321 tagttttttc tgttttcaaa ttaggattat ttaataaatc agcattcatt tgtctaatat
     7381 atagatattc aataggattt aatctgtttg aataggaagg acgttttgat aaaatatcat
     7441 agtgagcaaa gaataaagct tcgtcagata ttgggaaaga gaaagaaata cgtggcatta
     7501 ctactatttg aggtttgtag tcctcgaaag cttttgtatt taattcttct tttggattaa
     7561 ttaaatatgg tgcaatacca tttggtgatg caataagatt tgggttttca atttcaatac
     7621 cgcttgaatt ataccaaaca tttccaacac ggtaaccatt gattgctgta ggatttgata
     7681 aatcattaac ataaacagta gctccgttag gaatattttc tggatgagtt gaatttgcta
     7741 acaaacctag ttcatctgta tctcctaatt tatatgcttc atgtagtaaa tactggtctt
     7801 ttaaaaccat ttggttagca tcgtagcggt caacacgtac acctaagtta aatataaggt
     7861 ctttaaacgc aaacttatct tggatgtaac cagcaacata tgaaggctca aaaggagcga
     7921 taggacgtgt tttatatcct ttttcgtctg ttttgttaaa gaagtcgtca aatgttggtt
     7981 tgctatgtag tttattacct tgatagtcat atccataata agctactaca ggacttcctt
     8041 ggttaaataa ttcatcagca ctaaaccatg aaatatcaaa agtagatgga tcataagagt
     8101 caacatcaat ccatgttaaa tcatttattt ccatacctaa actttctctg aaatatttat
     8161 caaacctaga ttgaccttga gcattataaa gtcttgggta gtcaatagta tctaagaagt
     8221 ttccttggtc atcataatat attataggtt tagaaatatc tttttccaaa atatgcatgt
     8281 ttgttaatct gcgtgctaaa gtccataagc caaatggagc aactgcataa taagagtctt
     8341 ttctttgttc aaactcaaaa cctaaactaa attcgtgaga tttgatatcc gctgttcctg
     8401 atgcagatac tctaaattgt tgtccctcag atttttcaaa gcttgcataa ggatttcctg
     8461 gacttgcgta cattccataa attgaaggag ctccatttcc attcatcaat ccaccataac
     8521 ttaaaagatg gtcaacatct ttgtaataat aatcagagaa ataatcaaaa tattgttcag
     8581 tatatcttgc taagtcttta tttagttcat ttgtagtaaa gtcggtaatt ctataaaaat
     8641 atgtttcaaa taaattacct tctagacctg taacagaatc aataccccaa atataatatt
     8701 tttcaggtac tgttgtaaaa ttaccaatat gaccatattc aaaataattg tctttaaatt
     8761 ttgaatcgaa ataattgctt gaagatcttg aatagtcaaa ctgtatagta taatatgggt
     8821 tcttaattac agcagtatct tctgttctaa atttttgagt aatacgtgcg tatgtattcc
     8881 aagcatcgga aatataatta ccattattgt gtgagttgaa aagttgattt ccatagctat
     8941 ataatctacc tgctccaata tcaaaagttc ctccaatagt gaagtttaag tctcttatag
     9001 gagagatatc tatttttgct gcaacattgg cacctttacc accagcatta gctcttgatt
     9061 tataattttg gaaagcgtct gcatgtaaat agtcagagtt ttggtaaata atattaccag
     9121 attgcgactc aattaaacgc aaaggattag ttctaatttt ttctaaaaca tctgcatttg
     9181 ctctccattc acccactgct gatggtccac cgtcttttac tattctacct tctgcagcaa
     9241 gtaaaaatcc agcgatagtt ctttttctgt tagactcgcc tttttcagta accgaccaga
     9301 taggtcctga aaccattata gcagcaaggt tgtagccata tgggtctaaa tactgagaag
     9361 taacaacttc accacctccg tagaagtgtg aagaaggtcc tttagttgtt gcactaataa
     9421 ctccaccagt aacgtctcca tattgagcag gcaaacctcc agtaataact gtaacttgct
     9481 caatagcaga ttttggaact gccgagctac ctcttacttt aacgccgtca acgtagtaaa
     9541 cagtaccttc ggaacgagca ccacgaatac taccaacttc gccgtcttcg gtataaacac
     9601 cagcaactgt agttgcaact gctgctgcag aacgaccagg catttttgca aagtcttcag
     9661 aagttacagt tcctcctgat tgtgtttggt ctttagaaat taaaggaact ttatattctt
     9721 taatttctac ttcacctaaa atttctgctg ctgggtttaa gtcaaaattt tggtaaacta
     9781 tcttaccagc aataatttga acatttgtgt attgtaaagt tgcgtaacca atataagaag
     9841 cagaaacggt aaatcttcct gccggaatag gttttattga gaaatttcca tcgaaatctg
     9901 tcatacctcc ggtaacaata tttccgtttt cttcaatcgt tacgtttgca aacgggattg
     9961 gttcacctgt tgctgcatca gtaactttac cactcaaggt gcccgctgat tgagcataca
    10021 cccccgcact tgtaataagt gccactaaaa taaacagtaa ttttcgtatc ataataaagt
    10081 tttactttaa actaagtttg aaaaagtgta aaaatagaat agtttttttt attaaccaaa
    10141 aaaaaacgcc tcaaaataaa ttattttttt taaagatata tataagtact taataataag
    10201 acaaatcata aaatcatata ttttattctc tagtatattt tgctgatttt tttgattttt
    10261 tcattctttt ataagtacgt ttgccatcta ctacgtcttt accaccacca ttaattaatt
    10321 tattatgttt ttttacagct tttttatgta atcttgcatc tcttttcttt tttttagcat
    10381 ctttttttgc tttgtttttt tcaattttca tgactaatct ttcttcagga gaaggtcgat
    10441 aacgtttctg ccttaaagat ttcatatgtt tttcattgtc aatgcttcgt atgttttgac
    10501 taaaacttgt tgatgaacat aaaatgaaat ttattattag taagatttgt attatttttc
    10561 ttaactttgc cataaaatta aattagaaat gaaagtatat attgcaaaga taatcatttt
    10621 ttttacatgt gtaggttttt ttacaataat tcctgcatgt gaaaatgaag agcgtattcc
    10681 ttatgcttat gtagatttta ttctttattt agatatgcct caatattttg aagtaagaac
    10741 acctggtaat tatctttatc ttacaggtgg agtaaaaggc attattctgt attgtgaata
    10801 tgcagattct tatagagctt atgaaagaaa ttgtccatat aaaccttctg acccaaatgc
    10861 tatattagag gttgattcta ctggggtatt tttggtttgc cggcactgcg aatctaaatt
    10921 tttaataatg gacggaagtg ttgttaatgg acctgctaaa tatccagtat tgcaatatgc
    10981 tacctattta gaaggcggaa atagattgag tgtctttaat gctttttaaa acgaaataaa
    11041 aaagaggctg tttttacttt tgagacagcc tcttttttta tcatttacta aatttgtttt
    11101 aatatctata atgttcaggt ttgaatggtc cttctacagg aacaccaata taatcagcct
    11161 gttcttttgt tagttttgtt aatcttacac caatttgttc taaatgaagg cgtgcaactt
    11221 cttcgtcaag gtgttttggt aagcgataaa ctccaacttc atatttattt tgccataaat
    11281 ctaattgagc aagagtttga tttgagaaag agttactcat aacaaaagaa ggatgacctg
    11341 tagcacaacc taagtttact aaacgtcctt ctgctaaaag gaatatagaa tttccatttt
    11401 caaaagtata tttgtcaact tgtggtttta ttgttgttct ttttgctcct ttgtagttat
    11461 taaggttatc aacttgaatt tcattatcaa agtgtccaat attacataca atagcttgat
    11521 ctttcatttg tttcatatgt tcaagcgtga taacatctct gttgcctgta gtggtaacat
    11581 aaatatttcc ttcgttaaat gtgtcttcaa ttcttgcaac ttcaaaacct tccattgctg
    11641 cttgtaatgc acaaattggg tctatttcag taacaattac tctagctcca tatcctttca
    11701 ttgagcgagc acagcctttt ccaacatcgc caaaacctaa aacaacaaca acttttccag
    11761 caatcataac atctgtagct cttttaattc cgtctgctaa agattcatgg caaccataaa
    11821 gattatcaaa ttttgatttt gtaactgagt cgtttacatt aattgtagga actaaaagtt
    11881 cgcctttttc catcatttga tataaacgat gaactcctgt tgttgtttct tccgaaattc
    11941 cttttaagtc tttaaccgct ctagtccatt ttgaattatc aacagctaaa acttttttta
    12001 aggtttcaaa aattattctt tcttcgtcgc ttccgggttt tttatcaaga gttgaagcat
    12061 ttttttctgc ataataccct ttatgtacca ttaaggtagc atctccacca tcatctacta
    12121 tcaaatttgg acctaaacct cctggaaatg aaagagcttg caaagtacac caccaatatt
    12181 cttctaaggt ttcgcctttc caagcaaaaa caggaacgcc agcttttgct attgcagctg
    12241 cagcatggtc ttgagtagaa aaaatattac aacttgccca tcttacatca gcaccaagtt
    12301 cttttaaagt ttcgattaaa actgctgttt gaattgtcat gtgcaaagaa cctgtaatgc
    12361 gagcaccttt taaaggttta gaacctgcat attttttgcg gacactcata agtcctggca
    12421 tttctttttc tgcaatttca atttctttac gaccccaatc agctaggttg atgtctttaa
    12481 ttttgtatgg taatgagcta atcaattcgt tcataatatt atgtaattta atgttttatg
    12541 tttaaaatta aaaataataa gactgcaaaa ttatatgttt ttatttaaaa tcccaaattt
    12601 tttgaaaaat aggctaaagt ttcttcttgg tcttttttga tttgtgttat taaatcatct
    12661 atgttttcat attttatttc gtctctcatt ttttttataa attcgattgt aacttgcttt
    12721 ccatacaaat cgccttcgta atttggaata tgaaattcta gacttagttc tttaccatca
    12781 aaacttggac gtgttccttt gcttagcatt gcgggataag attttttgtc aaaatacgct
    12841 tttgcaacat aaactccatt aggtggctgt attttgtctt ggtctgaaac ttctaaattg
    12901 gctgttggaa atcctaattt gcgtccaatt tgataaccac taattatgtt gcccgaaagt
    12961 ttataattat agcccaacat tttattagct ttttctaagt cgccttgtgt aatacattca
    13021 cgaatttgag aagaattgat gtttgtaaat tcttcaaatt caaacttttg caatcgctta
    13081 acaggtatag caaattcgct ttcgggtttt gaagtggaat ttttattccc aaaatgattg
    13141 ttgtaaccca ttattataga tgagcatgaa agtttttctt ttaaaatatt ttccacaaac
    13201 atatttgcag ataaagaagc aagctcttcg ttaaaatcta aaattaaaac attttttatt
    13261 ccaagtccct caagaagtac gattttttcg ttaagagtat ttagtaaact tatattttta
    13321 ccaaaatatt ttgcagggtg gggccaaagt gtgataacaa gctcttcttg attttttgag
    13381 ttagcttcat tcattagctg tttcaataag taaacatgtc ctttatgaac accatcaaaa
    13441 aaacctatgc ttaccacaca attttttgga atatttttgt ttatatttgt aaaaactttc
    13501 aaaacatttt ttttgcaaaa atataaaatg aaaattaatt acacatattt atctctgaat
    13561 aaatctcgca cattaatatt aatggtatta atattattga gttttgtttt tttgttgtct
    13621 attatttctt gtaaaaataa tcataaacag gtcgaaattt ttggaataat cgaaaatgct
    13681 gaaaataaag aaatttattt agaaatgcta ttgccaggtc aaattaagcc tcttgttcaa
    13741 actgaaataa atgaaaaagg cgagtttaaa tttgaattag attctttaca aacttgtttt
    13801 tacagattaa aaatagatga aagaaacact atttatttaa gattaaatcc aaatgatgaa
    13861 attgagatta atgctagtta tccgaatatt caagcaaatt attctataga tggttctgaa
    13921 gaaagcaaaa atctaaaaga gctaaatatg tatttaatga aaagtacaaa aaggttaaat
    13981 gaaataaacg accaaattgt tgcagcttac aaaattccga attatgatgt tgattctctc
    14041 atcgaggttt taaatgttga ggcgagagct atgtataaaa acgataaaga gtttttaatt
    14101 aattttatta aaaaacataa tgaatctgtg attatttatt ttgctcttta tcaatatgtt
    14161 agcatttcgc caattttaat aatagaccaa gatatagaaa tttttcaatt tgtattagaa
    14221 gaattaaaaa aacataatcc agaatttgaa caaatagctt tattagagtc tgaaatttca
    14281 aaagagcttt tacgacaaca acagtttaat agggaatacg ggaaattaga aattggaaaa
    14341 cctgtagttg attttgattt gcctgatatt aatgggaata gagtttcgtt gtctaaattt
    14401 aaaaatcaaa aaattattct tgcgttttgg tcatcgtgga ataaagtgtc tttaaatgca
    14461 gtgcttgggc taagcaaaaa attagaaaat caagatgtgg ttttaatttt gatagctttg
    14521 gataatgcaa aagataaatg ggagacagcg gttaaaaata attttttgga aaaacatatt
    14581 aatttatctg atttaaaagc ttgggaaagt ccagttgcaa aaatttatgc agtaaaatca
    14641 attccaagtt ttttattaat tgacgaaaat caaaatctcg ttgcaataag tgatgattat
    14701 aatgaaatta ttgataattt aaacaaaaat taaaaaccca ttataattta atttatcttt
    14761 gaggcgtttt tataaacaat ttgaattttg ttttgaaaga aaaaaataaa atatactttg
    14821 tttcagactc tcacttcgga attgattatg ataaatatag ttctgaattt cgagaagaat
    14881 taatgttgtc gtggttggac gaaatcaaaa gcgatgcact agaactacat ttgtgtggag
    14941 atatgtttga tttttggttc gagtataaat atttagttcc acgaggcttt acgaggcttt
    15001 ttgcaaaact tagagaatta tctgatttag gtgtaaaact ttattattat cttgggaatc
    15061 atgatatgtg gaccttcggt tatattgaag aaactatagg tgcaaaagtt ttcaaaggag
    15121 ttaatctgaa agaaataaac ggcaaaattt tttacatagg gcatggcgat ggacttggac
    15181 cttatgacaa aaaatacaat tttttaaaaa aaatattttc ttctaagttt atgcaattca
    15241 tgtttaggtt agttcatcct tcaatatctt ttaggattgc aacaaactgg tcgtcttcaa
    15301 gtcgcaaaaa acataaatat cctaaacagg ttgatttcga aaaagaatgg cttgttagat
    15361 atgcaagaac tgtactcgaa aatcaaaaaa tcgattattt tatttttgga catagacata
    15421 ttccttttca atataatttg aacgcaaaca ctttgtttac aaatttaggc gactggcttt
    15481 ttaatttcac ttatgctgtt tatgatggcg aaaatctttt attgaaaact tatgaaaaag
    15541 ccgagtaatt tatttttaaa aactcatcaa tacataaaat cttcaaaaag aatttaattt
    15601 ggcaaatttt ttgttatttt tacaacttga aaatggttta attttaaaat aaataaaaat
    15661 atgaaaaaga ttttttactt ggtattagtg gtttttggat tttcttgttt tgcaaatgct
    15721 caaagcacaa aagttttaat tgaaacaagc atgggagata tgacagtagt gctttacgac
    15781 gacactcctg aacatcgtga taattttata aaattagcta acgaaggttt ttacgaagat
    15841 ttgctttttc atcgtgtgat aaatggtttt atgatacaag gtggagaccc tgattctaaa
    15901 ggagctgctc caaacaaacc tttaggaaat ggaggaccag gctatacact acctgctgaa
    15961 tttaatccaa aatatattca taaaaaagga gctttagcag ctgctagaac tggcgaccat
    16021 gtaaatcctg aaagacgtag ttcgggttcg caattttata tagttcaagg tgcaaaatat
    16081 accgatatgc aaatggatag catggaaaaa cagtttagaa gaaaatttac agacaaagat
    16141 agagata
//
LOCUS       DAOPNX010000059        16047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_4334_length_16047_cov_6.056466, whole genome shotgun sequence.
ACCESSION   DAOPNX010000059 DAOPNX010000000
VERSION     DAOPNX010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16047
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4334_length_16047_cov_6.056466"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PKN53_07490"
     CDS             <1..446
                     /locus_tag="PKN53_07490"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOD88827.1"
                     /translation="TLIGCLMLGNLLREAGVVDRLSKMVQNELINIVTLFLGISVGAT
                     ASADVFLTEQTIKIIVLGVIAFSFSTAGGVLIVKIMNLFSKEKMNPLIGSAGVSAVPM
                     AARVSQIEGQKADPSNFLLMHAMGPNVAGVIGSAVAAGLLLSFLG"
     gene            519..1229
                     /locus_tag="PKN53_07495"
     CDS             519..1229
                     /locus_tag="PKN53_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOD88828.1"
                     /translation="MKAMIFAAGLGTRLAPLTNDKPKALVEVGGKPMLEMQIENLKSY
                     GVKYIVINIHHFADQIINFIKSKDFGIEIHFSDESDLLLDTGGGLKNVKKYFQKGDNF
                     LVHNVDIYSDINLKDFYNYHITGNNLVTLAVKHRNSTKKLLFDENLQLCGWKSLSDGR
                     KIISIEKPQYDEMAFSGIYVFNYKIFDEITKTGAFPIIPELLEITKTQTIKAWVADNN
                     NILDLGKHEALIQYEKLI"
     gene            complement(1266..1673)
                     /gene="trxA"
                     /locus_tag="PKN53_07500"
     CDS             complement(1266..1673)
                     /gene="trxA"
                     /locus_tag="PKN53_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOD88829.1"
                     /translation="MKNLVLFFASIFLLGIFTFSELNAQEAKSNIKELTESNFNTSIK
                     KGLVLVDFYADWCRPCKMMQPILEDVATEYNSQITITKINIDNNKNISSKYNVTGIPC
                     MILFENGKEVKRVVGYHEKEQLLEKLADKVKMQ"
     gene            complement(1693..2610)
                     /gene="miaA"
                     /locus_tag="PKN53_07505"
     CDS             complement(1693..2610)
                     /gene="miaA"
                     /locus_tag="PKN53_07505"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOD88830.1"
                     /translation="MSKSNTLVIIFGATGVGKTDVSIEIANYFKSEIFSADSRQIYKE
                     LGVGVAKPSTEQLDSIKHHFISTVSIHEHYSIYQYEHDCINALTEYFTTNNIAVMVGG
                     SGLYIDAVLNGIDEMPDHDPEIREYVNELYEKNGIEYLRHELLKHDPEYYKIVDLKNQ
                     KRLIRALEIFYQTGKPFSQFRKNQAVERNFDTIKIGIDLDRNILYERINSRVDKMIKN
                     GLVEEAYDTYQYRNLPALKTIGYKELFEFIEKKRSLEDSIELLKKNTRNFARRQLTWF
                     RRYEDAKWFDLSEQNEISKYIESSLNFNK"
     gene            complement(2614..3141)
                     /locus_tag="PKN53_07510"
     CDS             complement(2614..3141)
                     /locus_tag="PKN53_07510"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88831.1"
                     /translation="MIIKYRLVFGKDDEFVRDIEIHEENNFLDLSLAIQAACNFDSSL
                     LSMFYLSNKSWDKLDEIVHDRIDAESQADALLMEETKLNHFEPKIGQRYIYIFDFFSE
                     RAFFIEIVNIRKVEKNDHLLEFPICTLSKGKAPEQIFIDDIDDEFEEDDFYDDDYDDD
                     GYDDIGFENIDDYDF"
     gene            complement(3144..4160)
                     /locus_tag="PKN53_07515"
     CDS             complement(3144..4160)
                     /locus_tag="PKN53_07515"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HOD88832.1"
                     /translation="MRNKIQILVNLGKNFSEVLENENTNNFPGFNSIYVANPWFIPEF
                     VKYSLKTWSKLLSEDKISAWLQNYNLPNFSTPKTLGLVFAGNIPMVGMHDFISGFICG
                     YNLKIKLSSKDNILMRWVIERLKTIGNISDEKIMIYDSLMGNFDKIIATGSNNSNRYF
                     EYYFAKTPNILRKNRTSIAILDGNETQKDLENLADDIFLYFGLGCRNVTKLYVPKNYN
                     FEALSKAIEKYSFLRNHNKYANNLDYRFAIFTMNKVPITNLGHLFLRENKDLQSYVSV
                     LNFEFYDKKEELIENINKKSEELQCVITKIDNIANRLDFGASQNPELYEYADNVDTIN
                     FLIK"
     gene            complement(4235..4759)
                     /locus_tag="PKN53_07520"
     CDS             complement(4235..4759)
                     /locus_tag="PKN53_07520"
                     /inference="COORDINATES: protein motif:HMM:NF040945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCC motif membrane protein"
                     /protein_id="HOD88833.1"
                     /translation="MTENDNNMNFNIPPIPDPEERGFNYNTYTNESSSSSSNNSNNQN
                     QDSNQNRGFDEASTNQAKNDLKPNIPNSGAILTLGILSLVTLLCCGACVSPILAIIAL
                     ALFPSAKNKYLQNPDLYSENSYSNIKTGKILAIVGLCISLFFIILLAIVGSFTESTEI
                     IKETFDSTWDTMGY"
     gene            complement(4854..6554)
                     /locus_tag="PKN53_07525"
     CDS             complement(4854..6554)
                     /locus_tag="PKN53_07525"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOD88834.1"
                     /translation="MQLFESYGLSKEILKAIKELEYINPTPIQAKTIPHLLNSDRDLV
                     GLAQTGTGKTAAFGLPILNQIDYQNKITQSIILSPTRELCVQIAKDMQNYAKYLPEIR
                     ITAVYGGASIENQIKDLRKGSQVVVGTPGRVLDLINRKVLKINQISFLVLDEADEMLN
                     MGFKEDIDAILETTPIEKQTLLFSATMPPEIMKIAATYMHDPLEISVGTKNSGAENVE
                     HFYYQVNAKNRYLALKRIADINPDIYGIVFCRTRAETQEVADKLIQDGYNADALHGDL
                     SQAQRDHVMHRFRSGNLQLLVATDVAARGLDVNDLSHIINYNLPDEREIYIHRTGRTG
                     RAGKKGEAITIIHNRELHKIREIEKILKKPINRRQVPDGKEVCTKQLMHIIDRIEHLS
                     EIDPEIDQFLPIILKKLSWLDRDELIKRFVAVEFSRFLTYYKNAYDLNEPDFKENKRS
                     NKAKSEGKSGYTKFFLTLGAKDGLNKGKLLDLINSNDNIAGADIGKIEIMPGYTFFEM
                     DSNYENEVIRAFNNKRYAGKRLKIEVKNSNKKEKPNFRKKDNKPTPKSDKKRQTGKRK
                     "
     gene            complement(6603..7595)
                     /locus_tag="PKN53_07530"
     CDS             complement(6603..7595)
                     /locus_tag="PKN53_07530"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOD88835.1"
                     /translation="MHSPIKFFAGSANRDLAIKIIDGYNQAQKQMHLESGNPIDTLKT
                     YSLGKSSFTRFSDGEFITSLDESVRGCTVFLIQSTFPPVDNLFELMLMVDAAKRASAY
                     KVVAVIPYFGFARQDRKDQPRVAIGAKLIADLLTAAKVDRIMTMDLHADQIQGFFNVP
                     VDHVYASALFVPYLKNLNLDNLVIASPDMGGSKRAKAYSHFLGCDMAIAHKSRVKANE
                     VGEMMVIGDVEGKNVIITDDMVDTAGTIVMAANIMMNQGAKSIRVVASHPVLSGPAYE
                     RLNNSPIIEFITTDSIPIKEGASDKIKVLSIGPLFGDIINKVYNFRSISGSFIV"
     gene            7849..8208
                     /locus_tag="PKN53_07535"
     CDS             7849..8208
                     /locus_tag="PKN53_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD88836.1"
                     /translation="MIKFFNLLILIFTANVLLAQHTLFFEIPECPTSSIGEFKNFNET
                     KIYPNPASEQLNIELKNFSTCKLEILDLNGRIISVENAKFENSTIKIDISNLEKGFYI
                     LQIRDKEKYFRKSFVKI"
     gene            8211..9428
                     /locus_tag="PKN53_07540"
     CDS             8211..9428
                     /locus_tag="PKN53_07540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOD88837.1"
                     /translation="MKNQIFKILIALIFILNFNFVAISQDYCHGDNFTLQATNYVAGT
                     VQWQYSYDDVNWYDFDNANSLTFYFIPDASMYVRLQIHDTSCIPEYYYAEKQYIELIP
                     QPTVADAGYDQLGIIGTSTVLEANQAEIGIGFWTIEEGQGGSLSDPNIPNATFTGLVG
                     ESYTLSWQIFNSCGSTEDFVKISFVSNFTCGQNIVDSRDGQTYPTVSIGGKCWLAKNM
                     NIGTMVFGTEQQTNTETIQKYCYANDENNCNIYGGLYQWNMAMQFTTSEGVQGICPEG
                     WHIPTDYEFKQLELALSMPSGEVDIMNSFRGHAQNVGGQMKQGGGSGFEALFAGARNG
                     GGGFMYLQGTGSYDFAYFWSSSHCSEGGECSEGRDENFAIRRCLQGGSQGSGRYDTFN
                     KGYGFSVRCVKNN"
     gene            9452..9817
                     /locus_tag="PKN53_07545"
     CDS             9452..9817
                     /locus_tag="PKN53_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88838.1"
                     /translation="MKTIKILLFVLIGSLSFTFLACDPDDPEPQPNPQPVIELEFISL
                     TADKELLYGGETATITAEARGEELVYQWTASAGSIVGSGKTVTYSPSPCSTGENTITC
                     KITDKYKESLSKSIIITVE"
     gene            9817..10791
                     /locus_tag="PKN53_07550"
     CDS             9817..10791
                     /locus_tag="PKN53_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88839.1"
                     /translation="MKNKFIVFFLFFGLLAFSNFEANAQCFASPGNPVAGSSNVGVLH
                     PRIFRLVLFHKFSYSDSYYSGSKFNEYDFPAAIKNANYNYTGMSLAYGLKPKLSLELD
                     LGYFINKTQNYRYIDYQLKGYGLSNIGLTAKYNIYKNPVKMLEITVGGGLKAPFRLEP
                     QVVNGVRLDVDVQPSTGNFGFNLQALFVKEFENISTRLIVLSRYDNNFTENRMGYKFG
                     VAFSNSIFISKHLANKYTELTKDITIICQFRHEYKKVNYLNGKVVKASGSNILYIAPQ
                     INYNFKMVWNFSFIFEKPIYHYYNETQLGNKYSLLLSITKDIGYKIKM"
     gene            complement(10863..10938)
                     /locus_tag="PKN53_07555"
     tRNA            complement(10863..10938)
                     /locus_tag="PKN53_07555"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10903..10905),aa:Phe,seq:gaa)
     gene            complement(11051..12772)
                     /locus_tag="PKN53_07560"
     CDS             complement(11051..12772)
                     /locus_tag="PKN53_07560"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOD88840.1"
                     /translation="MENIINKAKKWLDESFDAETRKEVQRMIDNDHALLTESFYKDLE
                     FGTGGLRGIMGVGSNRMNKYTVGMATQGLSNYLIKNFPNTKIKCAIAFDCRNNSDYFA
                     KISASVLSANGIKVYLFESLRPTPELSFAIRHLQCHSGIVITASHNPKEYNGYKAYWQ
                     DGGQIINPHDIGIINEVRNIKDLSQVKFDDNLKNVEIIGEDIDNAYINKLSELILNPE
                     DIKKSNLKIVFTPIHGSTVDILPKALRKVGFTDINIVEEQAIPDGNFPTVASPNPEEA
                     SALSMAIEKAKKIKADIVMGTDPDGDRLGIVVRQKNGEYLLLNGNQTASILIYYLLEE
                     WQKKGKLKGKEYIVKTIVTTELLSVIANHYNVKSYDVLTGFKYIAEIIEKNYGKTTFI
                     GGGEESYGFLAGEFVRDKDAVMSCMIMAEVAAWAKNRNITLDKLLIDIYIKFGYYLEG
                     LKSLTKTGKSGLEEIQKMMEKFRNQMPKSINGIDIALVHDFEKRISIDKRNNSKHEIN
                     LPKSNVLQFELVDGTKITIRPSGTEPKIKFYVSVHDVLKNKEEYPQITKELQAKIDKI
                     IETITNF"
     gene            13450..15363
                     /gene="dnaK"
                     /locus_tag="PKN53_07565"
     CDS             13450..15363
                     /gene="dnaK"
                     /locus_tag="PKN53_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOD88841.1"
                     /translation="MSKIIGIDLGTTNSCISVMEGNEPVVIANSEGRRTTPSIVGFLK
                     DGERRVGDPAKRQAITNPKNTIYSIKRFMGEKYDKLIDEYKRVPYDVVKGDNNTPRIK
                     IEDRLYTPQEISAIVLQKMKQTAEDYLGQTVKEAVITVPAYFDDSQRQATKEAGEIAG
                     LTVKRIINEPTAAALAYGLDKKNKDMKIAVFDLGGGTFDISILELGDGVFEVKSTNGD
                     THLGGDDFDHVIIEWLAAEFKKDEGLDLTKDPMALQRLKEAAEKAKIELSSSTSTEIN
                     LPYIMPVDGIPKHLVKTLTRSMFEQLADKYINATIKPCERALQDAGLTVSDIDEVLLV
                     GGSTRIPAIQKKVEEFFGKAPSKGVNPDEVVAIGAAIQGGVLTGEVTDVLLLDVTPLS
                     LGIETMGGVMTKLIEANTTIPTKKTETFTTAADNQPSVEIHVLQGERSIAQHNKTIGR
                     FHLDGIPPAPRGVPQIEVTFDIDANGILQVSAKDKGTGKQQSIRIEASSGLSDDDIKR
                     MKEEAQANAEADKVAKERIDKINQADSMIFQTEKQLKEFGDKIPADKRKPIEDALEKL
                     RQAHKDQNVDAIDAAMNELNTVFQAASQEMYNAQQSQTNAGPQDSGNTGNASNQQTGG
                     DDEVTDVDFEEVK"
     gene            complement(15446..15700)
                     /locus_tag="PKN53_07570"
     CDS             complement(15446..15700)
                     /locus_tag="PKN53_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88842.1"
                     /translation="MFIKKFSKTRVRYEYDQNALVYVIEVLPNEVYHLDNDYICWEND
                     LFNRFTTQFPTENICFISDDALVGIEKPELVIEGLNNHNK"
BASE COUNT         5381 a         2440 c         2567 g         5659 t
ORIGIN      
        1 ctacattaat tggttgtttg atgctcggaa acttattaag agaagcaggt gttgtggata
       61 gattaagcaa aatggttcaa aatgaattga ttaacattgt tactcttttc cttggaattt
      121 ctgttggtgc tactgcaagt gcagatgttt tcttgactga acaaacaatt aaaataatcg
      181 tattaggagt tattgccttt agttttagta ctgctggagg tgttttaatt gttaaaatta
      241 tgaatttatt ctcaaaagaa aaaatgaatc ctttaattgg ctctgctggt gtttctgctg
      301 tacctatggc tgcacgtgtt tcacaaatcg aaggtcagaa agctgatcca agcaatttct
      361 tgttgatgca cgcaatggga cctaatgttg ctggagtaat tggttcggct gtggcagcag
      421 gtttgttgtt gagcttcctt ggataaatta aaaatactat atattaaatg cctcgatttg
      481 gaggcatttt ttttatatat ttacaaaaaa atattattat gaaagcaatg atttttgcag
      541 ccggtttagg aacaagattg gctcctttaa caaacgataa accaaaggct ttagtagaag
      601 taggaggaaa gccaatgctt gaaatgcaaa tcgaaaatct taaaagttat ggagtaaaat
      661 atattgttat taatattcat cattttgccg accaaattat aaatttcata aaatcaaaag
      721 attttggaat agaaatacat ttttcagatg aaagtgattt gctattagat actggaggtg
      781 gattgaaaaa tgtaaaaaaa tattttcaaa aaggagataa ttttttagtt cataatgtag
      841 atatttattc agatataaat cttaaagatt tttacaatta ccatattact ggaaacaatt
      901 tggtaacact tgcagttaaa cacagaaata gtactaaaaa attacttttt gatgaaaatt
      961 tacagctttg tggctggaaa tctttatctg acggtagaaa aattattagt atagaaaaac
     1021 cacaatatga cgaaatggca tttagtggaa tttatgtatt taattataag atatttgatg
     1081 aaataacaaa aactggagca ttcccaatta tccctgaact tttagaaatt acaaaaaccc
     1141 aaactattaa agcttgggtt gctgataata ataatatttt agatttgggc aagcatgaag
     1201 ctcttatcca atatgaaaaa ttaatttaac agtcttgaaa agtgcgtaaa aaccatatag
     1261 ttagactatt gcatttttac tttatcagct agcttttcta aaagctgttc tttttcatga
     1321 taaccaacaa ctcttttcac ttctttacca ttttcaaata aaatcataca aggaatacct
     1381 gtaacattgt atttagaaga aatgttttta ttattatcaa tatttatttt agtaatggta
     1441 atttgagagt tgtactcagt tgcaacatct tcaagaatag gctgcatcat tttgcaagga
     1501 cgacaccaat ctgcataaaa atctactaag accaaacctt ttttaatact tgtattaaag
     1561 ttagattcag ttaattcttt aatgtttgat tttgcctctt gagcattcaa ttcgctaaat
     1621 gtaaaaatgc ctaataaaaa aattgaggca aaaaataaaa ctaaattttt catatcgttc
     1681 attaatttat tattacttat taaaatttaa acttgattca atatatttgc taatctcatt
     1741 ttgctcactt aaatcaaacc actttgcatc ttcatatctg cggaaccatg ttagttgtcg
     1801 ccgagcaaaa tttcgagtat ttttctttaa taattctata gaatcttcca aagaacgttt
     1861 tttctcaata aattcaaaca attctttata accaattgtt ttaagagcag gtaaatttct
     1921 gtattgataa gtatcgtaag cttcttccac caaaccattc tttatcattt tatcaactct
     1981 tgaatttatt ctttcataca gtatatttct atctaaatct atgccaattt ttatagtatc
     2041 gaaatttctt tcaactgctt gatttttacg aaattgtgaa aaaggtttgc cagtttgata
     2101 gaatatttct aaagctctaa tcagcctttt ttggtttttt aagtcaacaa ttttgtaata
     2161 ttctggatca tgttttaata attcgtgtct taaatattct attccatttt tttcgtaaag
     2221 ttcattaaca tattctctaa tttcgggatc atggtcgggc atttcgtcta ttccatttaa
     2281 aactgcgtct atatacaaac cagaacctcc aaccattacg gcaatgttgt ttgttgtaaa
     2341 atattctgtt aaagcattaa tgcaatcatg ttcatattga taaatactgt aatgctcatg
     2401 aatagaaaca gtgctgataa agtgatgttt tatactgtct aattgttctg tgctaggttt
     2461 tgcaacacca acgccaagtt ctttgtaaat ttgtcgcgaa tcggcagaaa atatttcaga
     2521 tttaaaataa tttgcaattt caatgctaac atctgttttt ccaacacctg tagcaccaaa
     2581 tattatgact aatgtattgc tttttgacat ttattaaaaa tcgtaatcat caatattttc
     2641 aaagcctata tcgtcatagc catcgtcatc gtagtcatca tcgtaaaagt cgtcttcttc
     2701 aaactcatcg tcaatgtcgt caataaaaat ttgttcgggt gcttttcctt tcgacaatgt
     2761 acaaatagga aattctaata aatggtcgtt tttttctact tttctgatat ttacaatttc
     2821 aataaaaaat gctctttctg agaaaaaatc aaaaatatag atatagcgtt gtccaatttt
     2881 tggctcaaaa tgatttaatt ttgtttcttc cattagtagt gcatctgctt gactttcagc
     2941 atcaattcta tcatgaacaa tttcatctag cttatcccaa gatttatttg atagataaaa
     3001 catgctaagt aggctagagt caaaattaca tgcagcttga attgctaaac ttaaatctaa
     3061 aaagttattt tcttcgtgga tttctatgtc tctaacaaac tcatcgtctt tgccaaatac
     3121 taatctatat tttattatca taattattta attaaaaaat ttatagtgtc aacgttatca
     3181 gcatattcat atagttcagg attctggctc gcaccaaaat caagtctatt tgcaatatta
     3241 tcaatttttg taataacaca ttgtaattct tctgattttt tatttatatt ttcaattaat
     3301 tcttcttttt tgtcgtaaaa ctcaaaattt agaacagaaa cataagattg caaatcttta
     3361 ttttctctta aaaataaatg accaagattt gtgattggaa ctttattcat tgtaaatatt
     3421 gcaaatctgt aatctaaatt atttgcgtat ttattgtgat ttcttaaaaa tgaatatttt
     3481 tcaatagctt tagagagagc ttcaaaatta taatttttag gtacataaag ttttgtaaca
     3541 tttctacagc caagaccaaa gtaaagaaaa atatcatctg ctaaattttc taaatctttt
     3601 tgagtttcgt ttccatctaa aattgcgata gaagttctat tttttcttaa aatatttgga
     3661 gttttagcaa aataatattc aaaataacga ttagaattat tgcttccagt tgcaattatt
     3721 ttatcaaaat ttcccattaa ggaatcgtaa atcataattt tttcgtcaga aatattgcct
     3781 atggttttta gtctttctat tacccatctc atcagaatat tatctttaga cgacaatttt
     3841 atttttaagt tatatccgca aataaaacca ctaataaaat cgtgcatgcc aaccatagga
     3901 atattaccag caaaaactaa acccaaagtt tttggtgtag aaaaattagg taaattataa
     3961 ttttgaagcc aagccgaaat tttatcttcg cttaataatt tactccatgt tttaagagaa
     4021 tattttacaa attcaggaat aaaccaagga ttagcaacat aaatactatt aaatccagga
     4081 aaattattag tattttcatt ttctaaaact tcagaaaaat ttttccccaa atttactaat
     4141 atttgaattt tatttctcaa ctttcaactt taccttcggt gagctcgcaa gctcggttca
     4201 actttcaact tttaactttt aacttttaac tgacttagta tcccatagtg tcccatgtag
     4261 aatcaaaagt ttctttaatt atttcagtac tttcagtaaa acttccaaca attgcaagta
     4321 ggattatgaa gaataaagaa atgcaaagac ctactatagc taaaattttt cctgttttaa
     4381 tatttgaata tgaattttca ctgtacaaat caggattttg tagatattta ttttttgcac
     4441 ttgggaaaag ggcgagagca attatcgcta aaattggaga aacacaagca ccacaacata
     4501 aaagagttac taatgataaa attcctaaag ttaaaattgc accagagttt ggaatattag
     4561 gtttcaaatc atttttagct tgatttgtgc ttgcttcatc aaaacctcga ttttggttag
     4621 aatcttgatt ttgattattt gaattattgc tagaactact gctactttcg ttagtgtaag
     4681 tattatagtt gaagcctctt tcctctgggt ctggaatagg aggaatatta aaattcatgt
     4741 tattgtcatt ttcagtcata atagtatatt tttatataga ctacaaatgt acaaaattaa
     4801 tttaagttaa aaatattttt aaactatatt tcaatattaa aatgaagagt tttttatttt
     4861 cttttgccag tttgtctttt tttatctgat tttggagtgg gtttgttatc tttttttcta
     4921 aaattaggct tttctttttt gttagagttt tttacctcta tttttaaacg ttttccagca
     4981 tatcttttat tattaaaagc tcttataact tcattttcat aattggaatc catttcaaaa
     5041 aaagtatagc ctggcataat ttcaattttt cctatgtcag caccagcaat attgtcgtta
     5101 gaatttatta aatctaaaag tttaccttta ttcaagccat cttttgcacc aagagttaag
     5161 aaaaacttag tatatcctga ctttccttca ctttttgctt tattgcttct tttattttct
     5221 ttgaaatcgg gttcgtttaa gtcgtaagca tttttataat aagttaaaaa tctagaaaac
     5281 tcaactgcta caaatcgttt tattagttca tctctatcta gccagcttaa tttttttaaa
     5341 attataggca aaaattgatc tatttctggg tctatttcag aaagatgttc aattctatca
     5401 ataatatgca taagttgctt tgtacaaact tctttaccat cgggaacttg tctgcggttt
     5461 ataggttttt ttagaatttt ttcaatttct cgaattttgt gcagttctct attgtgaatt
     5521 attgtaattg cttcgccttt tttccctgct ctgcctgtac gtccagttct gtgtatataa
     5581 atttctcttt cgtcaggaag attataattt ataatatggc taaggtcgtt tacatcaagt
     5641 ccacgagcag caacatcggt agcgacaagt aattgcaaat ttccacttct aaatctgtgc
     5701 ataacatggt cgcgttgtgc ttgcgataaa tctccatgca aagcatctgc attataacca
     5761 tcttgaataa gtttatctgc aacttcttga gtttcggcac gagttctaca aaatacaatt
     5821 ccgtaaatat ctggatttat gtcagcaata cgttttaagg ctaaatatct gtttttcgca
     5881 tttacttgat agtaaaaatg ttcgacattt tctgctcctg aattttttgt tcctactgaa
     5941 atttctaaag gatcgtgcat ataggttgca gcaattttca taatttcggg tggcatggtg
     6001 gcagaaaata ataaagtttg cttttctatt ggtgtggttt ctaaaatagc atcaatatct
     6061 tctttaaatc ccatgtttag catctcatca gcttcgtcta aaaccaaaaa agatatttga
     6121 ttaattttta aaacttttct atttatcaaa tctaaaactc tgccgggtgt gccaacaaca
     6181 acttgtgaac cttttcttaa atctttaatt tgattttcga ttgacgcacc gccataaacc
     6241 gcagttatac gaatttcggg caaatatttt gcataattct gcatgtcttt tgctatttgc
     6301 acacaaagtt cgcgagtggg cgaaagaata attgattgtg taattttatt ttgatagtcg
     6361 atttgattga gaataggcaa accaaaagca gcagttttac cagtaccagt ttgagctaaa
     6421 ccaactaaat ctctgtcaga atttaatagg tgaggtattg tctttgcctg aattggcgta
     6481 ggattaatat attctaattc ttttatggct ttaagaattt ctttacttaa accatagctt
     6541 tcaaataatt gcatttctta aaatttcagg ttgcaaagat aaacaataat taataaaatt
     6601 gtctaaacaa taaaacttcc tgaaatactt ctgaaattat ataccttatt aattatatcg
     6661 ccaaaaagtg gacctataga aagtactttt attttatccg atgctccttc ttttattgga
     6721 atagagtcgg ttgtaataaa ttcaataatt ggtgaattat ttaatctttc gtaagcagga
     6781 ccagacaaca ctgggtgaga tgcaaccact ctgatagatt tagcaccttg gttcatcata
     6841 atatttgccg ccattacaat agttcccgca gtatcaacca tgtcgtcagt aattattaca
     6901 tttttgcctt ccacatcacc gatcaccatc atttcgccta cttcgtttgc ttttacgcgt
     6961 gatttatgag caatagccat gtcgcaacct aagaaatgag aataagcttt tgcacgtttc
     7021 gagccaccca tgtcgggtga agcaattact aaattgtcta gatttaaatt ttttagatag
     7081 ggcacaaaca aagcagatgc gtaaacatgg tcaacaggaa cattgaaaaa tccttgaatt
     7141 tgatctgcat gtaaatccat agtcataatg cggtcaactt ttgcagctgt gagtaaatcg
     7201 gcaattaatt ttgcaccaat agcaacgcgt ggctggtctt ttctgtcttg acgagcaaaa
     7261 ccaaaatatg gaattactgc tacaacttta tatgccgaag ctcgttttgc agcatcaacc
     7321 ataagcataa gctcaaacaa attatctaca ggtggaaaag tactttgaat taaaaatact
     7381 gtacaaccac gaacagattc atctaaactt gtaataaatt cgccatcact aaaacgtgta
     7441 aaactacttt tacctagact ataagttttt agagtatcga taggattacc cgattctagg
     7501 tgcatttgtt tttgagcttg attataacca tctataattt ttatagctaa atcgcgattt
     7561 gccgaaccag caaaaaattt aattggtgag tgcatctttt ataaattttg tttattgatt
     7621 gcaaaattac aaaaataaaa gaagtttgta aaaattaatc tgagattgat attaactagt
     7681 tttcaacaaa attaaattca attttaatat ataaaatcat tcttgtaaaa tgatgaaaat
     7741 cagccatata gatttaaagt gataaagttt cttataattt tatttgttct atttattact
     7801 attacataaa ttttttgtaa cttagcaaag tatttaaaaa gttttgttat gattaaattt
     7861 tttaatcttt taattttaat atttacagca aatgtattgt tagcacaaca tacattgttt
     7921 tttgaaattc cagaatgtcc tacaagtagc ataggagaat ttaaaaactt taatgaaact
     7981 aaaatttatc caaatccagc atcggagcag ttgaatattg aattgaaaaa cttttcaact
     8041 tgtaaattgg aaattttaga tttaaacggc agaattatta gtgttgaaaa tgcaaaattt
     8101 gaaaattcaa ctattaaaat tgatatttca aatctagaaa agggatttta tatcctgcaa
     8161 atacgtgata aagaaaaata ttttagaaaa agttttgtaa aaatttagcc atgaaaaatc
     8221 agatttttaa aatattaata gctttaattt ttatccttaa ctttaatttt gttgcaatat
     8281 ctcaagatta ttgtcatgga gataatttta cattacaagc cacaaactat gttgcaggaa
     8341 ccgtgcaatg gcaatattct tatgatgatg tgaattggta tgattttgat aatgcaaact
     8401 cgctgacttt ttattttatt cctgatgcta gtatgtatgt gcgtttacaa atccatgata
     8461 caagttgtat ccctgaatat tattatgccg agaaacaata tattgaatta atacctcaac
     8521 ctacagtagc agatgcaggt tatgatcagc tcggaattat tggtacttca acagttttag
     8581 aggctaatca agcagaaata ggtataggct tttggacaat tgaagaaggg cagggtggaa
     8641 gcctttcaga tccaaatatt ccaaatgcaa cttttactgg cttggttggc gaatcttata
     8701 ctttaagttg gcaaattttt aattcttgcg gaagtactga agattttgtt aaaattagtt
     8761 ttgtgagtaa ttttacttgc ggacaaaata ttgttgacag tagagatgga caaacttatc
     8821 caacagtttc tataggtgga aaatgttggc ttgcaaaaaa tatgaatata ggaacaatgg
     8881 tttttggtac tgaacaacaa acaaatactg aaactattca aaaatattgt tatgcaaacg
     8941 atgaaaataa ttgcaatatt tatggaggtt tatatcaatg gaatatggca atgcagttta
     9001 caacaagtga aggtgtacaa ggaatttgtc cagaaggttg gcatatacca acagactacg
     9061 aatttaaaca attggaatta gctctaagta tgccaagtgg ggaagtagat attatgaata
     9121 gttttagagg acatgctcaa aatgttggtg gtcaaatgaa acaaggtggc ggttcaggat
     9181 tcgaagcatt atttgctgga gcaagaaacg gaggcggagg ttttatgtat ttgcaaggta
     9241 ctggctctta tgattttgcg tatttttggt ctagcagcca ttgtagcgaa ggtggcgaat
     9301 gtagtgaagg acgtgacgaa aattttgcaa taagaagatg cttgcaaggt ggaagtcaag
     9361 gctctggaag atatgatacc tttaataaag gatatggttt ttcggtaaga tgtgtaaaaa
     9421 ataattaata atatattaaa atctaaatat tatgaagact ataaaaattt tactttttgt
     9481 tttaattgga agtttaagtt ttactttttt ggcttgtgat cctgatgatc cagaaccaca
     9541 gccaaatcca caaccagtaa tagaattaga atttatatca ctaactgctg ataaagagct
     9601 tttatatggt ggcgaaactg caactattac tgccgaagct cgtggcgaag aattggttta
     9661 tcaatggacg gcttcagcag gttcaattgt tggctctgga aaaactgtta catactcgcc
     9721 atcaccttgt agtacaggag aaaatactat aacatgcaaa attacagata aatataagga
     9781 atccttgtcg aaaagtataa ttattaccgt agagtaatga aaaataagtt tattgtattt
     9841 tttttgtttt ttggattatt agcgtttagc aattttgaag ctaatgctca atgttttgca
     9901 tcgcctggaa atccagttgc aggaagttct aatgtaggag ttctgcatcc aagaattttt
     9961 agattagtgc tttttcataa attttcttat tccgattctt attacagcgg ttcaaagttt
    10021 aacgaatatg attttcctgc agcaataaaa aatgcaaatt ataattatac aggcatgagc
    10081 ttggcttatg gactaaagcc aaaactgagc ttagaattag atttgggata ttttataaat
    10141 aaaactcaaa attatagata tattgattat caacttaaag gctatggttt gtcaaatatt
    10201 ggattaactg caaaatacaa tatttataaa aatcctgtaa aaatgctcga aattactgtg
    10261 ggcgggggac ttaaagcacc atttagatta gaaccgcaag tagtaaacgg agttagacta
    10321 gatgtggacg tgcaaccttc aacaggaaat tttggcttta atttgcaagc tctgtttgtt
    10381 aaagaatttg aaaatataag cactagactt attgtgcttt ctcgctatga caataatttt
    10441 acagaaaata gaatgggata taaatttgga gttgcttttt caaattcaat ttttatttct
    10501 aaacaccttg caaataaata cacagaacta acaaaagata ttacaattat ttgccagttt
    10561 aggcatgaat acaaaaaagt taattatttg aatggaaaag tagtaaaagc atcgggaagt
    10621 aatatccttt acattgcacc gcaaataaat tataatttta aaatggtgtg gaatttttcc
    10681 tttatttttg aaaaaccaat ttatcattat tacaacgaaa cacaactcgg caacaaatat
    10741 tcacttttgc taagtattac aaaagatatt ggatataaaa taaagatgta ggtaattgtt
    10801 tgattttgag cgagttgaaa atccttataa aataaaaaac cccaacttta gtcggggcgc
    10861 tgtggtggta ccacctagaa tcgaactagg gacacacgga ttttcagtcc gttgctctac
    10921 cgactgagct atggtaccta aatcggactg caaaaataga gcaaattttt aaatatccaa
    10981 aaattttagc agaaaaaaat aaaaatattt gctcaaactt tgcaacttat tgattttcaa
    11041 atgtaaactt ttaaaaattt gttatggttt ctattatttt atcaattttt gcttgtagtt
    11101 cttttgttat ttgtggatat tcttctttgt ttttaagcac atcgtggaca gaaacgtaaa
    11161 atttgatttt tggctcagtt cccgaaggtc gaatagtgat ttttgtgcca tcaactagtt
    11221 caaattgcaa aacattagat tttggaagat ttatttcgtg ctttgaattg tttcgtttat
    11281 ctatacttat tcttttttca aaatcgtgaa ctaaggcaat gtcaatacca tttattgatt
    11341 ttggcatttg atttctgaac ttttccatca ttttttgaat ttcttccaag ccacttttgc
    11401 cagtttttgt taaagacttt aagccttcta aataatatcc gaattttata taaatatcaa
    11461 ttagcaattt atctaaagtg atatttctat tttttgccca tgcagcaact tctgccataa
    11521 tcatacaaga cattacagca tctttatcgc gaacaaattc tccagctaaa aatccataac
    11581 tttcttcgcc accgccaata aaagtggttt taccataatt cttttcaatt atttcggcaa
    11641 tatatttaaa acctgtcaaa acatcataag attttacatt ataatgattt gcaattacag
    11701 ataataattc agtagtaaca atagttttaa caatatattc tttgcctttt agttttcctt
    11761 ttttctgcca ttcttccaaa agataataaa tcaaaataga agcagtttga tttccattta
    11821 aaagcaaata ttctccgttt ttttgacgca caacaattcc taatctatcg ccgtctgggt
    11881 cggttcccat aacaatatct gctttaattt tctttgcttt ttcaatagcc atactcaaag
    11941 ctgaagcttc ttcgggattt ggcgaagcaa cagttggaaa attgccatca ggaatggctt
    12001 gctcttctac aatattaata tctgtaaaac caacttttcg caaagctttt ggcaaaatat
    12061 caacagtaga accatgaata ggagtgaaga ctatctttaa attagatttt tttatgtctt
    12121 caggatttaa aatcaattca gaaagtttgt taatataagc attgtcaatg tcttcaccaa
    12181 taatttcaac attttttaaa ttgtcatcaa atttaacttg agataaatct ttgatatttc
    12241 ttacttcgtt gataattcca atgtcgtgag gatttataat ttgtccgcca tcttgccaat
    12301 aagctttata gccgttatat tcttttggat tgtgagaagc tgtaataaca attcccgaat
    12361 ggcattgtaa atgtcttatc gcaaaagata attcaggtgt gggacgcaga ctttcaaata
    12421 aataaacttt tataccattt gcagataaaa ccgaagcaga aattttcgca aaataatctg
    12481 aattattcct gcaatcaaaa gctatagcac attttatttt tgtatttgga aagtttttta
    12541 tcaaataatt gcttaaacct tgagttgcca ttccaacagt atatttattc attctgttgc
    12601 taccaacacc cataatgcca cgcaagccac ctgtgccaaa ttccaaatct ttataaaaag
    12661 attctgtcaa aagtgcatgg tcgttgtcta tcatgcgttg tacttcttta cgagtttctg
    12721 catcgaaact ttcgtctaac catttttttg ctttgttgat tatgttttcc ataaattatt
    12781 ttttttgtaa agttagtttt tttataaacg atgtgataaa attaatgaaa atttttaaaa
    12841 ctatggctat aaaataaaaa ataattgaat ttttttagtc agatttcctc tgctcgctcg
    12901 gatttgcaat ccgagtgatt ggcaatgtta cactcggatt gcaaatccga gcgagcgtca
    12961 tgggtgttag atgcgagcgt catgggtgtt agatgcgagc atcagggctg ttggaagcta
    13021 tctattgact atcaaaggct tgtccgtagg acaacatgtg attaaccgta ggcgcagcct
    13081 gcggtattta gaccctcaaa agccttgccc gtagggcaac acaacacttt taacctttaa
    13141 cactagtgca tcgcttttaa cacttcaact acgttcagtg catcgctttt aactgaagta
    13201 cccttgcgga caaggagtaa ttgttcgaat tggtgctaca actatgaaaa ctggaatagt
    13261 tttttgtcat aacttgaatt ttcttagtcg gatttctgac aaattgtcat tgttttttaa
    13321 attaatttca aaaatcttga attttgtcag tcatttaatc atataaactt aaaaaaatag
    13381 cattggcaca acaattgact aattaagtgt gaaaataaaa ttattaacta aaatttaaaa
    13441 agaataaata tgagcaaaat tataggaata gatttaggaa caacaaactc atgtatatct
    13501 gtaatggaag gaaatgaacc tgttgtaata gcgaacagtg aaggtcgtag aactacgcca
    13561 tcaatagttg gtttcttaaa agacggagaa cgtagagttg gtgatcctgc aaaacgtcag
    13621 gcgataacaa atcccaaaaa tacaatatat tctattaagc gttttatggg agagaagtat
    13681 gataaattaa tagatgagta caaacgcgtt ccttacgacg tagtaaaagg ggataataat
    13741 acgccaagaa ttaaaattga agataggctt tatacgccac aagaaatttc ggcaattgtt
    13801 cttcagaaaa tgaaacaaac agctgaagat tatttaggtc aaactgttaa ggaagctgta
    13861 attacagttc ctgcatattt tgatgattct caacgtcaag caacaaaaga agctggcgaa
    13921 attgcaggtt taacagtaaa acgtattatt aacgagccta ctgccgctgc tttggcttat
    13981 ggtttggata agaaaaataa agatatgaaa attgctgttt tcgacttagg aggcggtact
    14041 tttgatattt ctatcctcga acttggtgac ggagtgtttg aagtaaaatc aacaaatggc
    14101 gatactcacc ttggtggtga tgattttgac catgttatta tcgagtggtt agcagctgaa
    14161 tttaaaaaag acgaaggtct cgatttaaca aaagacccaa tggctttaca aagactaaaa
    14221 gaagctgcag aaaaagcaaa gattgaattg tcaagttcta caagtacaga aatcaatctt
    14281 ccttatatta tgcctgttga tggtattcca aagcacttgg taaaaacttt aactcgttca
    14341 atgtttgagc aattggcaga taaatatata aatgcaacaa ttaaaccttg cgaaagagct
    14401 ttacaagatg ctggactaac agtttctgat attgacgaag tgcttttagt tggtggttca
    14461 acacgtattc cagctattca gaaaaaagta gaagaatttt ttggaaaagc tccttctaag
    14521 ggagtaaatc ctgacgaagt tgttgctatt ggagctgcta ttcaaggtgg tgtgttgact
    14581 ggtgaagtta cagacgtgtt attattagat gttactccac tttcattagg aattgaaact
    14641 atgggtggcg ttatgactaa attgattgaa gctaacacaa ctatacctac taagaaaact
    14701 gaaactttta ccacagcagc tgataatcag ccatctgtag aaattcatgt gctacaaggc
    14761 gaaaggtcta ttgcacagca caacaagact attggacgtt tccacttaga tggaattcca
    14821 ccagcaccaa gaggagtgcc tcaaattgaa gtaactttcg atattgatgc aaatggaatt
    14881 ttacaagttt cggcaaaaga taagggaaca ggaaaacaac aaagtattag aattgaagcg
    14941 tcatctggtt tgtctgatga tgatattaag agaatgaaag aagaagcaca agctaatgcc
    15001 gaagccgaca aagttgcaaa agaacgcatt gacaaaatca atcaagcaga ttcaatgatt
    15061 ttccaaacag aaaaacaatt gaaagaattt ggagataaaa ttcctgccga caaacgcaaa
    15121 ccaatagaag atgctttaga aaaattacgt caagctcata aagaccaaaa tgttgacgct
    15181 attgatgcag caatgaatga gctaaacaca gttttccaag cagcttctca agaaatgtat
    15241 aatgcacaac aatcgcaaac taacgcaggt cctcaagatt ctggaaatac tggaaatgct
    15301 tcaaatcaac aaactggtgg tgatgacgaa gttacagatg tggactttga ggaagtaaaa
    15361 taaaataatg taaaaactga aataataaaa agctgtctct cttgaaggga cagctttttt
    15421 tgttcataaa aaattaatat ttttgttatt tgttatgatt attcaatcct tctatcacta
    15481 attcaggttt ttcaattcct actaatgcgt catcagaaat aaaacatata ttttctgtag
    15541 gaaattgagt tgtaaatctg ttgaaaaggt cattttccca acatatataa tcgttatcta
    15601 agtgataaac ctcgtttggt aatacttcaa taacataaac caaagcattt tggtcatatt
    15661 cataacgaac acgtgtttta gaaaattttt tgataaacac ttttaattgg tttcaaaatt
    15721 ttgtatcttt gtggtctgaa acagtagtga taggctctaa gctcatcatg ccgtgttgtg
    15781 aattggtttc aaaattttgt atctttgtgg tctgaaacag tgctcgatag gctcatagtt
    15841 tacgtctgaa gttgtgaatt ggtttcaaaa ttttgtatct ttgtggtctg aaacagtata
    15901 ttctggttgt tgcgcttgaa tttcaggttg tgaattggtt tcaaaatttt gtatctttgt
    15961 ggtctgaaac agtgaagcga ttaagctact cggaattgca accgttgtga attggtttca
    16021 aaattttgta tctttgtggt ctgaaac
//
LOCUS       DAOPNX010000060        16029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_4340_length_16029_cov_7.000876, whole genome shotgun sequence.
ACCESSION   DAOPNX010000060 DAOPNX010000000
VERSION     DAOPNX010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16029
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4340_length_16029_cov_7.000876"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1121
                     /locus_tag="PKN53_07575"
     CDS             72..1121
                     /locus_tag="PKN53_07575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HOD88843.1"
                     /translation="MKNICRNIISIFLFFGVVFYLNSCKKEEIPTLKTGEVSNITENS
                     ATLSAKVSKDGDSEIIVRGFVWNTTKNPSLEANIGFSENGSGVGDFTHNVEGLMSETT
                     YYVRAYATNEIGTAYGNEVSFTTNAPSWNGTPCTDCETITDIDGNIYRTVKIGTQCWL
                     ADNLKTTKYNDGTEIQNVTLNTEWNVLSTGAFCWYNNDANNKENYGALYNYYAVETGK
                     LCPEGWHVATDNEWKELEGFVDSEYKVGNSIWNNESWRGSDVGIRLKASYFWLGGSQQ
                     DKSDSHGFSALPGGVRNGDTGGFERLEEWGLWWSSKNDVETNVYRRNLEKNKDNIARF
                     PSNPKSGYSIRCIKN"
     gene            1125..3761
                     /locus_tag="PKN53_07580"
     CDS             1125..3761
                     /locus_tag="PKN53_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD88844.1"
                     /translation="MKFFINSILILLLSCSVFAQNWSVHFSPRFRVLNDVHIHNPEEI
                     VIIGGHPFNDSITYMAYSSTAGQTWDIFTDIFPGKMINTAIFKNNITGYCAGQNEAFY
                     KTIDGGKTWSPFNIGINLNHKNINKLFKSEYNVFFATGGLNNENGFLLKSSDGGNTWS
                     SIYNWASNEIITAASPKTNTIIVGGLNGFLQISTDGAESWQNCNYPNLEILPEITGIN
                     FFDNNNGFCCGGVRGQDSTAIILKTTDGGSNWTISLNTTAPRLNDICMASEQVIYAVG
                     DYGTVLKSEDGGTSWNEENIDGNPGVDLFAVNFLNTHLGAISGRYGYVMIFDDGQTNL
                     PEVSTLEATKVTTSSAILNAVINPGFSDSEVSFLYGTNDNTEHEINCGTFSGGELKSI
                     EKSISNLETDTKYYFRAKLNNSYGEYYGNIKYFYTGNPIPNWDFEEWIEISEDFPESW
                     YIANAFFERVTYEGFDAIRLQKENNLDEEKDFDVWRQGLTNTLIKGNGPPQTEWQLPI
                     NFIHGGLPLNTRPNTLFLTSKYNIEPNDTSAVFIGLILDDHYVAENIFFLTGSQQNFE
                     TNAFPIEYSTDEIPEKAVIVIVSSNPFDKLNSYDSYIEISEIHFSDNTPEIPNSDFSS
                     WITKTTHLPQDWYMKIIHNYDGSIKNYIERVTDAAHNDYAIKLQNIITEQDTITAQLS
                     LRDYNEGMPIEKRFNKLMGYFKYFPESNDTARISIVMYQGGSPIAWGQYLTNETIDSW
                     TEFNIDIQYHHTHQFTIPDSMNISFASSQYPPTGESQLFIDKLSLDGDFIPVKQYNIN
                     DLNLYPNPASDFINIDISNIIISTNLSYEIFDITGKLIVKEILDTNCKLIEINLNNFP
                     NGLYSLQLLTDNSIYTSKFLKL"
     gene            3758..8248
                     /locus_tag="PKN53_07585"
     CDS             3758..8248
                     /locus_tag="PKN53_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD88845.1"
                     /translation="MRKQVLLILFSLISVYILGQTQIATPTGTTITTSGSRVDETNGI
                     TYTYTNSSWPSGCFAWGFNRSNTSLGCINGNDTPPWNCANYFTSSYSISSNVAYLNTS
                     TTIRYVNTDGNYVNGTVNARLKFTATTTGGVAINFVQSGNIIFIPFTSNFIIKVEMEL
                     QGPTNAIWGTLANGNNYSGMWSAADNIYDALNTDPTYSIYTSYDNAYTYFYSLPTSST
                     TPTITSSCGSAVTSSSFTFTWSGDCTIHPNSHYEYSFNGGAWTNNGTTNSKTVTLNVG
                     QNSFQVRYYDGCKAQYYTSDICYIYYAPDNYCGDINHGGNNWTISSNTTVSGNHTNVG
                     TFTINSGDTATVNPSCHYFYVSANNIVINGTIDANGAGEGGGNGGNGGAQAFGNTDNC
                     NGGYGGISGTTGSGSGGGSIGGSGGNGSCRKQKCGGFLCSGNRDGFMGGGGGAGGGGG
                     GGYGGTGGEGGLGAYGASWNEASGGSYGSGGNAGIAYGTPTGTDINWGSGGGGAGGGG
                     GAFDNGTNGGRGGNGGGAIKLIASNNCTVTGTISSNGTSGAAGGNGGQTSVAQGYSCS
                     STGYNSCPICPEKNYDAPGGAGGGAGGGSGGGIMIQASGIMTFTGSIYASGGTGGPAG
                     LPNENYGNCHYNAQGGAGGGGGRVKIFVNPCLNNITSGTVSVSGGNGGSGYSTGNNGS
                     SGTYNVINLSMPYPTVTASASPSSICSGGSSTLSASGASTYTWSHSLGSGSSKTVAPT
                     STTTYSVTGTNQYGCTGSTSVTVTVVADPAAPTLSVATPSNGNEVCVGGIVKATFTAG
                     SGGTGCTDEYQYSVNGGSTWNSYSPGSNITATTTGANAIQIQTRRPCSGIGCDGAGTT
                     WTTKAQWTVVANPSITVSGATTMCPGLNTNLTANGSGGTGTSTYQWQYYNGSSWINVG
                     TNSSAYTTDNLINSTDFRCIYSTNGTGCTDANSNTVTVTIQTPNTADLSSNDYVWYGS
                     GSTNWTTASNWLKYNGTNFETATAIPTVNDNVLITHFGTPSTCVSNLPYVAVASGECN
                     SIKINPNAQVFINNSKKLTIKGNLNNAGTITSNDGSIDIAGNWTNTGVFNAGTGTVTF
                     NGNTTQTINPGPASSAFNNVVFNNTASGISDININQPCSINGTATFTNGVVNFPGSGS
                     LSFGTSASSNIGNANSYVNGVISKTGTSAFNFATGEGEHWGPCAIAAPATNSTITAQY
                     KHSAGPLNWSAAYMCSGSDLHHTSGVEHWNLSSNESTPNVTLYWNSDSDVVDPNELVI
                     SHFNSSTNCWENKGATNITGGASSGSITNAIPFTSYSPITLGTKTAINPLPVEFTFFS
                     AECDNKNNVQLYWETASEINNDYFEIQRSTDDLVWEVIANIQGAGFSNTTLYYDYLDK
                     ENPNANTYYRLRQVDFDGRSKFSDIISIRCENRMDKPIISVYPNPFNSTLNIEFENWD
                     MKSAEIELVDITSRTIKTWKLENTVPNFVYEVNLSNLNPAMYMLRIKTSSGVIMRKIE
                     KQ"
     gene            complement(8428..10824)
                     /locus_tag="PKN53_07590"
     CDS             complement(8428..10824)
                     /locus_tag="PKN53_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88846.1"
                     /translation="MSNKFLQSKKKDKILLLAILVFTSFVFLGVKNFSFVNWDDDENI
                     GTDSSFTELSVENFTKHYQKDRYKALAIWSFMLEYQVFGYNPKYYHLDNLILHLLNVV
                     FVFILIKRLVPKNRFAPLIVSVFFAVHPAFVEPIAWVTGRKDLLFVLFSLLSILSYLK
                     FLKIEKQHLKYLMFGLTGVLVYLASLSKIQAVALPFLLFLIDWLECRKFSFSSLLEKL
                     IIFLLIVDLFKTLLFILLFVCVLKFYRPLFMIMSKNLWTKIISSIVLFLIILFLKTYV
                     KEALCHYMSYKSALVLSEVFLWLGYFLILFLMFFQEISKKIRLFFSKLFPYEKYRILI
                     VALIAIAFILVFQYEYILIWFPRLWTIDAGNPLYFTISERFLLLGSSLLYYLSRFFLL
                     KSQDPMIPYPSRLADGSLPNYMFFDLGIILLIMGISLYVLIKYFRKNRIVWFGVLFFL
                     INIGVVLHIIPIEGRVLAADRYTYLAYIGLFLIMALIADYFIEKKRGKIISGIIGLSV
                     IFMSYTTYQDKNTWKNSFTLWEKALKVNPKNHFAMYSLALANFTEANDIDNAEKLLDK
                     AINLKEDFMYYNNRGRIHYAKGNYTLALDDFDKSILLDSANSAAYNNRGAVRQQFCDF
                     IGALSDYKKAVELNPDYQDALNNIKRVENLLSIDSVLFEKSENKAISEVDILNFVLYI
                     SEKMLNTGQKPKAILYLEQAIKVIPNNYEIYERLALIYHLNEEFSEAKDVYNQALDKL
                     GKNESLLLGRGLLNIQIGDTVSACKDFKQSAELGNDRAKLLEQEFCGDTLSVKRVLSF
                     "
     gene            complement(10994..13147)
                     /locus_tag="PKN53_07595"
     CDS             complement(10994..13147)
                     /locus_tag="PKN53_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOD88847.1"
                     /translation="MKRILAILLGFTIIFNLNLRADEGMWLLPLIGKNYEDMKKQGFR
                     LSPEDIYSVNNSSIKDAIIGLGKEGSPFWHFCTGEIVSDQGLFFTNHHCGYGMIQSHS
                     TVEHDYLKDGFWAYSQDQELANPGTTASILVRIEDVSEQILSKLNDGMSEKERQEIID
                     KEGEALSAKAVEGTPYKAYVTDMFNNNQFFLFVYVIYQDVRLVGAPPSSIGKFGGDTD
                     NWMWPRHTGDFAMFRVYTGPDGNPAPYSEENIPLKPKHFLPVSNKGVKDGDFAMIIGF
                     PGTTNRYITSFGLEETMNVTNKLRFEIRDVKVKVLRAEMRASQKTKIQYASKYASTSN
                     YWKYSEQQNKALKHLNTMQIKKEIEAEYLKVNTDPKYKEALNLLEKGYKDRYDYAVAR
                     MYLLEGLISGPELPFFAYKCSGLLSALESKDEERINKLVEAIKQEAESFYKDYNPDTE
                     KKVEIALFNYVYNNLDKTYHPDFFNTIEKKYKLNFDKYVNDMFNKSVFATKEKLFAFL
                     ENPNLKTLQKDLVLSTGISILSKYREVNTQYWKNSDDIDKGSRIFTEGILNMNKGKLM
                     APDANSSIRLTYGQVRKYDPRDGVTYDYYTTIEGVMQKEDPESTEFEVPARLKELYKA
                     KDFGPYVNEKGELSVCFISNNDITGGNSGSPVINGDGHLIGLAFDGNSEAMSGDIDFE
                     ENLQRCINLDVRYALFIIDKYAGAKNLIKEMKIVN"
     gene            13243..14133
                     /locus_tag="PKN53_07600"
     CDS             13243..14133
                     /locus_tag="PKN53_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88848.1"
                     /translation="MQTFIKTVFSLSLFLLTHNFLFSQVTVPISEKEMEQYNRERQAI
                     MAMKIDLSQTWLYMVKNNVISSNKILLSEVFYSKQGLPELVVQYDENQKITSSTRVKY
                     NSKNLPFEEIKFAADSSLIGGILFEYDKNGLLNKQVHYNESGVITAIQQYLRKQDTIF
                     MSVFNNSAEFIYGNALIFENKNGQSLLKSMYKIDSSHNIIEKNLFDYDEIYALRSKIV
                     YDDKDLGTQKNFVYNDDGALVRTIITNSAGIHISDTSFEYDEYGNLIRIVEYEEETGT
                     AKLFFINYLSLVKEENGNKN"
     gene            14117..14863
                     /locus_tag="PKN53_07605"
     CDS             14117..14863
                     /locus_tag="PKN53_07605"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOD88849.1"
                     /translation="MEIKTKIYRRQQQVRSYDIDENLELKLNVIFQWFSEIAWEHAKI
                     LGIGFEELKNVDKFWVLNSVNVEINKLPKWQDQLILETWPSGISGIQYTREFQFYDDK
                     QNVLIAASSSWVIIDKNTLRPILPPEFSYLDELLKEKATDNLLKKIPLHKDLKFETEE
                     TARYTDIDMHHHVNNAVYVNWIENIMGNILPNKIKRFKIQFSNELKLGDKVSIFTKKE
                     NNYIYWESLKSDDKVCFRAEAEFENNLEIN"
     gene            14876..15505
                     /locus_tag="PKN53_07610"
     CDS             14876..15505
                     /locus_tag="PKN53_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88850.1"
                     /translation="MELTALEILGYIASILIAWSMAVSSIIKFRWINLFGALGLTIYS
                     IIISAIPVAIVNALIVILDIYYLYKAYTKNEVFQTLELSEDDHYLKQFIEFYKNDIKE
                     FFPNFEYKPDSKNLQFLILRDMYVTGVFMAKKIDDKTYNVDLDYVRPEYRDFKNGKYI
                     YSQIKEKFLIQSCTTVKTQSKNKAHIKYLNKIGFKKVGEGEFEMKLKNN"
BASE COUNT         5672 a         2455 c         2658 g         5244 t
ORIGIN      
        1 atctttacca caaattaata caagttatca taaacaacct tgtgaaactt gacatttata
       61 aatattaaat tatgaagaat atttgcagaa atattatttc tatttttttg ttttttggag
      121 ttgtatttta tttaaactct tgcaaaaaag aagaaattcc aactttaaaa actggcgaag
      181 taagtaatat tactgaaaat tcagccactt tgtcggcaaa agtaagtaaa gatggagata
      241 gtgaaataat tgttcgtggc tttgtatgga atacgactaa aaatcctagt cttgaggcaa
      301 atattggatt ttcagaaaat ggtagtggcg taggagattt tactcataat gttgaagggc
      361 ttatgtcaga gaccacctat tatgtacgcg cttatgcaac aaatgaaata ggcacagcat
      421 atggaaatga agttagtttt acgacaaatg caccatcgtg gaatgggaca ccttgcacag
      481 attgtgaaac gataactgat atagatggca atatttatcg tactgttaaa attggtactc
      541 aatgctggtt agccgacaac ctaaaaacaa caaagtataa tgatggaact gaaatccaaa
      601 atgttacact aaatacagaa tggaatgtgt tatctacagg tgcattctgt tggtataaca
      661 atgacgcaaa caataaagaa aactatggag ccttatataa ttattatgct gtagaaaccg
      721 gcaaattatg tcctgaaggc tggcatgttg ctacagataa tgaatggaaa gaattggaag
      781 gttttgttga ctctgaatat aaagttggta atagtatttg gaataatgaa agttggagag
      841 gtagtgatgt tggaataaga cttaaagcat catacttttg gttaggtggc tctcaacaag
      901 ataaaagcga ttctcatgga ttttctgcac tgcctggcgg tgttagaaat ggagacacag
      961 gaggttttga acgacttgaa gaatggggat tatggtggtc tagcaaaaat gatgtagaaa
     1021 ccaatgttta tagacgcaat cttgaaaaaa acaaggataa tattgcaaga ttcccaagta
     1081 acccaaagag tggctattct ataagatgta ttaaaaatta ataaatgaaa ttctttataa
     1141 attccatatt aattttactt ttaagctgtt cggtttttgc tcaaaattgg agtgttcatt
     1201 tcagtcctag atttagagtt ttaaatgatg ttcacattca taatcctgaa gaaatagtta
     1261 ttattggtgg gcatccattt aacgattcga taacttatat ggcgtattca tcaactgctg
     1321 gacaaacttg ggatatattt accgatatat ttcctggcaa aatgataaat actgctattt
     1381 ttaaaaataa tatcacaggt tattgtgcag gacagaacga agctttctat aaaactatag
     1441 atggtggtaa aacttggtca ccttttaata taggaataaa tttaaaccat aaaaatataa
     1501 acaaactttt taaatcagaa tataatgtgt tttttgctac cggaggttta aataatgaaa
     1561 atggttttct actaaaatct tctgacggtg gcaacacatg gtcatcaatt tataactggg
     1621 cttctaatga aattattact gccgcatctc caaaaaccaa tactataatt gttggcggat
     1681 taaatggatt tttacaaatt tctactgatg gagccgaaag ctggcaaaac tgtaattatc
     1741 caaacctaga aatacttcct gaaattacag gaataaactt ttttgataat aacaatggtt
     1801 tttgttgcgg tggtgttcgt ggacaagact ctactgccat aattttaaaa acaacagatg
     1861 gcgggagcaa ctggacaatc tcattaaata cgacagctcc acgcttaaat gatatttgta
     1921 tggcttcaga gcaagtaata tatgcagttg gtgattatgg aactgtactt aaatctgaag
     1981 atggaggaac ttcttggaat gaggaaaata ttgatggcaa tccaggagta gatttatttg
     2041 ctgtaaattt cttaaacacc caccttggag caatttcagg aaggtatggt tatgttatga
     2101 tatttgatga tggacaaaca aacttgccag aagtatcaac attagaagca acaaaagtta
     2161 ctacaagttc tgccatttta aatgctgtaa tcaaccctgg attttctgac tcggaagtaa
     2221 gctttcttta tggcactaat gacaatactg aacatgagat taattgcgga acattttctg
     2281 gaggagaatt gaaatctatt gaaaaatcaa taagcaatct tgaaactgat acaaaatact
     2341 attttagagc taaattaaat aatagttatg gtgaatatta tggcaatata aaatattttt
     2401 acacaggcaa tcctattcca aactgggatt ttgaagaatg gatagaaata tctgaagact
     2461 ttccagaaag ttggtatata gctaatgctt tttttgaaag agttacatac gaaggttttg
     2521 atgcaataag gctacaaaaa gaaaataatc ttgatgagga aaaagatttt gatgtttggc
     2581 gccagggact tacaaatacc ttaattaagg gtaatggacc accacaaaca gaatggcaat
     2641 tacctattaa tttcattcat ggaggtttac cattaaatac acgaccaaac actttattct
     2701 taacttcaaa atataacata gaacccaatg acacatcggc agtattcatt ggtttaatat
     2761 tagacgacca ttatgttgcc gaaaacatct tctttttaac agggtctcaa caaaattttg
     2821 aaaccaatgc ctttccaatt gaatactcaa cagacgaaat tccagaaaaa gcagtaattg
     2881 tgattgttag ttcaaatcca tttgacaaat tgaatagtta cgatagctat atagaaatat
     2941 ctgaaataca cttttctgat aatactccag aaattccaaa ctcagacttt agcagctgga
     3001 taactaaaac aacacatcta ccacaagatt ggtatatgaa aataattcat aattatgacg
     3061 gatcaattaa gaattacatc gaaagggtta ctgatgcagc tcataatgat tatgcaataa
     3121 aattacaaaa tataattaca gaacaagata caataactgc acaattatct ttaagagact
     3181 ataacgaagg aatgcctatt gaaaaacgtt ttaataaatt gatgggatat ttcaaatatt
     3241 ttccagagtc aaatgatact gcaagaattt caatagttat gtatcaagga ggctctccta
     3301 tagcttgggg acaatatctc actaatgaaa caattgatag ttggacagaa tttaatattg
     3361 atattcagta tcatcataca catcaattta caattcctga ttctatgaat atttcatttg
     3421 cctcctctca atacccacct acaggagaat cccaactttt tatagataag ttaagtttag
     3481 atggagattt tattcctgtt aaacaatata atataaatga tttaaactta taccccaacc
     3541 ccgcatcaga ttttataaat atagatattt ctaatattat tatttctaca aatttatctt
     3601 acgaaatttt tgatattaca ggcaaattaa ttgtaaaaga aattttggat acaaattgta
     3661 aacttataga aataaatctt aataattttc caaatggttt gtattcatta caattattga
     3721 ctgataattc aatatatact tcaaaattct taaaattatg agaaagcaag tacttttaat
     3781 attattttca ctaattagtg tttatatttt aggacaaaca caaatagcaa ctccaacagg
     3841 tacaacaatt accacatctg gttcaagagt tgatgaaaca aatggtataa cttatacata
     3901 tacaaattct tcttggccat cgggttgttt tgcttgggga tttaatcgta gcaatacaag
     3961 tttgggttgt ataaatggaa acgatactcc accttggaat tgtgccaatt attttacatc
     4021 aagttatagt atttcaagta atgttgcata tttaaatact agcacaacta ttaggtatgt
     4081 caacactgat ggtaattacg ttaatggaac agtaaacgca agacttaaat ttacagcaac
     4141 tactactggt ggagtagcaa ttaattttgt gcaaagcgga aatataattt tcattccttt
     4201 cacaagcaac tttataatta aagtagaaat ggaattgcag ggaccaacaa atgctatatg
     4261 gggaacccta gcaaatggaa ataactatag tggaatgtgg agtgctgctg ataatatata
     4321 tgatgcttta aatacagatc caacttatag catatacaca agttatgata atgcatatac
     4381 atatttttac tcattaccaa cttcttcaac aacaccaaca attacctctt catgtggtag
     4441 tgcagtaact tcctcttcgt ttacttttac ttggtcaggc gattgtacta ttcatccaaa
     4501 ttcacattat gaatatagtt ttaatggcgg tgcatggaca aataatggca ctacaaattc
     4561 aaaaacagtt acattaaatg taggtcaaaa tagctttcaa gttagatatt atgatggctg
     4621 caaagctcaa tattatactt ctgatatttg ttacatttat tatgcaccag ataattattg
     4681 tggtgatata aatcatggtg gtaataattg gactatcagc tcaaatacta ctgtcagtgg
     4741 aaatcataca aatgttggta catttacaat aaatagtggg gatacagcta cagtaaatcc
     4801 ttcatgtcat tatttctatg tatcagcaaa taatatagta atcaatggta ccatagatgc
     4861 taatggtgct ggagagggtg gtggtaatgg tggtaatggt ggagctcaag catttggcaa
     4921 tactgataac tgtaatggag ggtatggtgg tatcagtgga accactggta gtggttcagg
     4981 tggtggttct attggaggta gtggaggtaa tggaagttgt cgtaaacaaa aatgtggtgg
     5041 tttcttatgt agtggaaatc gagatggctt tatgggtgga ggtggaggtg caggtggagg
     5101 tggtggaggt ggctatggag gcacaggtgg agagggagga ttaggtgctt atggagcaag
     5161 ttggaatgag gcttctggag gtagttatgg aagtggagga aatgctggaa ttgcttacgg
     5221 cactccaact ggtactgata taaattgggg atctggtggt ggtggagcag gtggtggtgg
     5281 tggagcattt gataatggca caaatggagg acgcggtggc aatggtggag gtgctattaa
     5341 gctaatagct agcaataatt gtactgttac tggtactata tcttctaatg gcactagtgg
     5401 tgctgcaggt ggtaatggtg gacaaacatc tgtagcgcaa ggttattcat gttcgtcaac
     5461 aggttataat tcttgtccta tttgtccaga aaaaaattat gatgctcctg gtggagcagg
     5521 tggtggtgct ggtggcggct ctggtggtgg tattatgata caagcaagtg gtataatgac
     5581 ttttactggt tcaatatatg cgagcggagg tactggtggt ccagcagggt tacctaatga
     5641 aaattatggt aattgccatt ataatgccca aggtggagct ggtggtgggg gtggcagagt
     5701 aaaaattttt gttaatcctt gtttaaacaa tattacttct ggtacagtat ctgtatctgg
     5761 tggaaatggt ggtagtggat atagtacagg taataatggt tctagtggca catataacgt
     5821 tatcaatctt agtatgccct accctactgt gacagcatca gcatctccat cttctatatg
     5881 ctctggagga agttcaactt tatcagcatc tggtgctagc acttatacct ggtctcatag
     5941 ccttggtagt ggctctagca aaacagttgc cccaacatct accactactt attcggttac
     6001 tggtactaat cagtatgggt gtactggaag tacatcagtc actgttacag tagtggcgga
     6061 cccagcagca ccaacactaa gtgttgcaac cccttcgaac ggaaacgaag tttgtgttgg
     6121 cggtattgta aaagcaactt ttactgctgg aagcggtggt actggttgta cagatgaata
     6181 tcaatattct gtaaatggtg gtagcacatg gaatagttat tcgccaggaa gcaatattac
     6241 agcaacaact acaggagcaa atgctattca gatacaaaca cgcagaccat gttctggcat
     6301 tggttgcgac ggtgctggga caacttggac tacaaaggca caatggacag tagttgcaaa
     6361 tccaagtata acagtatctg gtgcaacaac aatgtgccca ggattaaaca caaatctaac
     6421 tgcaaatggt tctggtggta caggaacatc aacatatcaa tggcaatatt ataacggatc
     6481 ttcttggata aatgtaggaa caaattcatc agcatataca acagataatt taataaattc
     6541 tacagatttt agatgtattt attctactaa tggcactggt tgcacagatg caaattcaaa
     6601 tacagtaaca gtaactatcc aaactccaaa tacagcagat ttaagctcaa atgattatgt
     6661 ttggtatgga tcaggaagca caaactggac aactgcatct aactggttga aatataatgg
     6721 tactaatttc gaaacagcta ctgctattcc aactgtaaac gataatgttt taattacaca
     6781 ttttggaaca ccttcaactt gtgtttctaa cttgccatat gttgctgtag cttcaggaga
     6841 atgtaattca attaaaataa acccaaatgc tcaagttttt ataaataatt caaaaaaatt
     6901 aactataaaa ggtaatttaa acaatgcagg aactattaca agcaacgatg gaagcattga
     6961 tattgctggc aactggacaa atacaggggt ttttaatgct ggtacaggaa cagtaacttt
     7021 taatggaaat actactcaaa ctataaatcc tggtcctgct tcatctgcat ttaataatgt
     7081 tgtgtttaac aatactgcaa gcggtatttc tgacattaat ataaatcagc cttgtagtat
     7141 aaacggaaca gcaacattta ctaatggagt tgttaacttc cctggttcag gatcactttc
     7201 ttttggaaca tctgcctcat cgaatattgg aaatgcaaat tcttatgtaa atggagtaat
     7261 ctcaaaaaca ggaacttctg catttaattt tgcaacagga gaaggagaac actgggggcc
     7321 ttgtgctata gctgcacctg cgacaaactc aacaattaca gcacaatata aacattctgc
     7381 tggtccttta aattggtctg ctgcttatat gtgttctggt tctgatttac atcacacaag
     7441 tggagttgaa cattggaatc tatcatcaaa tgaatcaaca ccaaatgtaa cattatactg
     7501 gaattcagat agtgatgtag ttgatcctaa tgaattggtt atatctcact ttaattcttc
     7561 tacaaattgt tgggaaaata aaggagctac aaatattaca ggtggtgcaa gctctggaag
     7621 tataactaat gctattccat ttacaagtta tagtcctatt acacttggta ctaaaactgc
     7681 cataaatcct ttacctgtag aatttacctt tttctctgca gaatgtgata ataaaaacaa
     7741 tgttcaactt tattgggaaa ctgctagtga aataaataat gactattttg aaattcaacg
     7801 ctcaactgat gacttagttt gggaagtaat tgcaaatatt caaggtgcag gattttcaaa
     7861 tactacattg tattatgatt atttggataa agaaaatcca aacgcaaata cttattatcg
     7921 cttacgtcaa gttgattttg atggaagatc taaattctca gatataattt ctatacgttg
     7981 cgaaaatcgt atggataaac ctataatctc agtttatcca aacccattta actctacatt
     8041 aaatattgaa tttgaaaatt gggatatgaa atctgctgaa attgaactgg ttgatattac
     8101 tagccgaact ataaaaactt ggaaattaga aaatactgta ccaaattttg tttacgaagt
     8161 aaatctaagt aatctaaatc cagcaatgta tatgcttaga attaaaactt ctagtggggt
     8221 gattatgagg aagatagaaa aacaataatt agttttgagt ttttagtttt tagtttttag
     8281 ttgggagatt ttaaaaactt cacaatgata aaatttcttt agtgcagggt ttgtctatac
     8341 ttgcttcgca gaatttacaa gcccactgaa agcaaaagtt tttaaaattt cccacccact
     8401 ctgcacttac caactaataa ctcaaaacta aaaactcaaa actcttttaa ctgataaagt
     8461 atcgccacaa aattcttgct ccaacaattt tgccctatca tttccaagtt ctgctgattg
     8521 cttaaaatct ttacacgcac ttactgtatc gccaatttga atatttaata atcctcgtcc
     8581 taaaagtaaa ctttcgttct taccaagttt atctaatgct tgattgtaaa catcttttgc
     8641 ttccgaaaac tcttcattta agtgataaat taatgcaagt ctttcataaa tttcataatt
     8701 gtttggaata acttttattg cttgttctaa atataaaatt gctttcggct tttgaccagt
     8761 atttaacatt ttttcagaaa tatataagac aaaatttaat atgtccactt cgcttatggc
     8821 tttattctca gacttttcaa acaaaacact atcaatgctt agtaaatttt ctactcgctt
     8881 aatattattt aatgcgtctt gataatctgg atttagctca actgctttct tatagtcact
     8941 taaagctcct atgaaatcgc aaaattgctg acgaaccgct cccctattgt tatacgctgc
     9001 tgagtttgct gaatcaagta aaattgactt atcaaaatcg tcaagtgcta aagtatagtt
     9061 cccttttgca taatgaattc ttcccctatt attataatac ataaaatcct cttttagatt
     9121 tattgcttta tctaatagtt tttcagcatt atcaatatca ttcgcttctg taaaattagc
     9181 taaagccaaa ctatacatag caaaatgatt tttgggattt acttttaatg ccttttccca
     9241 aagagtaaaa gaatttttcc aagtattttt atcttgataa gttgtgtatg acataaatat
     9301 aactgacaag ccaataattc cagaaataat ttttcctctt ttcttttcta taaaatagtc
     9361 tgcaattaaa gccattatta aaaacaagcc tatataagcc aaatatgtat agcgatctgc
     9421 agccaaaact ctaccttcaa taggaattat atgaagtaca acaccaatat ttattaaaaa
     9481 gaataaaact ccaaaccaaa ctattcgatt ttttctaaaa tattttatta aaacataaag
     9541 agaaattccc ataattagta aaattatgcc taaatcgaaa aacatataat ttggtaaaga
     9601 accatctgcc aaacgactag gataaggaat cataggatct tgagatttta ataagaaaaa
     9661 tctactcaaa taatacaaca aactggaacc gagaagtaaa aaacgttcag aaatggtgaa
     9721 ataaagtgga tttcctgcat ctatagtcca taatcgtgga aaccaaatta atatatactc
     9781 atattgaaag accaaaataa acgcaatagc aattaaagca acaattaata tacgatattt
     9841 ttcatatgga aataacttag aaaaaaacaa tcttattttt ttagatattt cttgaaaaaa
     9901 cattaaaaac aaaattaaaa aataacctag ccaaagaaac acctctgata aaacaagtgc
     9961 tgacttgtat gacatgtaat gacaaagggc ttcttttaca taagttttta aaaataaaat
    10021 tattaaaaaa agtactatgc tagatataat ttttgtccaa agatttttag acataatcat
    10081 aaaaagtggt ctataaaatt ttaacacaca gacaaaaagt aatataaata ataatgtttt
    10141 aaataaatca actatcaata aaaagatgat taatttttct aacaaacttg aaaaactaaa
    10201 ttttctacac tctaaccaat caatcaggaa aagaagaaaa ggcagagcaa ccgcctgtat
    10261 ttttgataag ctagctaaat aaactaaaac acctgtaagt ccgaacatta agtactttaa
    10321 atgttgtttt tcaattttta aaaactttaa ataactaaga attgaaagca agctaaacaa
    10381 cacaaaaagc aaatcttttc gacctgtaac ccatgcaatt ggttcaacaa acgcaggatg
    10441 aacagcaaaa aagacactga caattaaagg agcaaatctg tttttaggca caagcctttt
    10501 tattaaaata aaaacaaaaa caacgtttaa taaatgtaat attaaattat caagatgata
    10561 atattttgga ttatatccaa aaacttgata ttcaagcata aaagaccata tagccaaagc
    10621 tttatatcta tctttctgat aatgtttagt aaaattttct acacttaatt ctgtaaaact
    10681 tgaatctgta ccaatatttt catcatcatc ccaattaaca aagctaaaat tttttacacc
    10741 caaaaacaca aaactagtaa aaaccagtat cgctaaaaga agaattttat cctttttttt
    10801 tgactgtaaa aatttattgc tcataagatt ataaaacaaa gttcaaagta aaaatataat
    10861 ttaaggtgtc ttataaatat tcaaatgtaa ggcatctgag attgaggacg aaggagcgta
    10921 caagaggtac gtgactgagt ccgaatatcg aatgcaacgc agcagttgga tatttataag
    10981 acaccgttta tttttaattc acaattttca tctctttaat aagattctta gctcctgcat
    11041 atttatcaat aataaacaaa gcatatctta cgtcaagatt tatacatctt tgtaaatttt
    11101 cttcaaaatc aatatctcct gacattgctt cgctatttcc gtcaaaagct aatcctatta
    11161 aatgtccatc accgttaata actggagaac ctgaatttcc gccagtaata tcgttattgc
    11221 tgataaaaca aactgaaagt tcgccttttt cgtttacata aggaccaaaa tcttttgctt
    11281 tatatagttc tttaagtctt gctggaactt caaactctgt gctttctggg tcttcttttt
    11341 gcataactcc ttctatagtt gtatagtaat cgtaagtaac accatcgcga ggatcatatt
    11401 ttcttacttg accataagtt aaacgaatgc ttgagttcgc atcaggagcc ataagttttc
    11461 ccttattcat atttaaaatt ccttcggtaa aaattcttga gcctttatct atatcatctg
    11521 agtttttcca gtattgagtg ttaacttcac gatatttact caatatgctt attcctgtgc
    11581 taagaactaa atctttttgc aaagttttta aatttggatt ttctaaaaat gcaaataatt
    11641 tttctttagt cgcaaataca gacttattaa acatatcgtt tacatattta tcaaaattaa
    11701 gtttgtattt tttctcaata gtgttaaaga aatctggatg atatgttttg tctaaattat
    11761 tgtaaacgta attaaataaa gctatttcaa ctttcttttc tgtatctgga ttatagtctt
    11821 tatagaaaga ttctgcttct tgcttaatag cttcaactaa tttattaatt ctttcttcat
    11881 ctttagattc taaagcactt aataatcctg aacatttata tgcaaaaaat ggcaattctg
    11941 gtccagaaat taatccttca agcaaataca ttcttgctac tgcataatca tatctatctt
    12001 tatagccttt ttcaagcaaa tttaaagctt ctttatattt tgggtcggta ttaactttta
    12061 aatattctgc ttctatttct tttttaattt gcatagtatt taaatgtttc aaagctttat
    12121 tttgttgttc gctgtatttc caatagtttg atgtagaagc atattttgaa gcatattgaa
    12181 tttttgtttt ttggcttgct ctcatttctg ctcttaaaac cttaactttc acatctctaa
    12241 tttcaaatct aagtttattt gtaacattca tagtttcttc caagccaaaa gatgtgatat
    12301 atctgtttgt tgttcctgga aaacctataa tcattgcaaa atcgccgtct ttaacgcctt
    12361 tgttacttac aggtaaaaaa tgtttaggtt taagaggaat attttcttca gaatatggtg
    12421 ctgggttgcc atcaggacca gtataaactc taaacatagc aaagtcgcct gtatgacggg
    12481 gccacatcca gttgtcggtg tcgccaccaa attttccaat agaagatgga ggagcgccaa
    12541 ctaaacgcac atcttgataa attacataaa caaataagaa aaactgatta ttgttaaaca
    12601 tatctgttac ataagctttg taaggagtgc cttctacagc ttttgcagaa agagcttcgc
    12661 cttctttatc aattatttct tgtctttctt tttcactcat tccatcgtta agttttgaca
    12721 aaatttgttc gcttacatct tcaatacgaa ctaaaataga agcggtggtt cctggatttg
    12781 ctaattcttg atcttgtgaa tatgcccaaa aaccatcttt taaataatca tgttcaactg
    12841 ttgaatgaga ttggatcatt ccataaccgc aatggtgatt tgtgaaaaac aaaccttggt
    12901 cagacactat ttcgccagta caaaaatgcc aaaacggact gccctcttta cctaacccga
    12961 taatagcatc ttttatgcta gaattattta cactgtaaat atcttccgga ctaagtctga
    13021 agccttgttt tttcatgtct tcgtagtttt tacctattag tggcaacaac cacattccct
    13081 catctgctcg taagtttagg ttaaaaatga tagtaaaacc taataaaatt gctaaaattc
    13141 ttttcattgt tttaagtatt tattatattt ttgaacaaaa ataactcaag ttttttgttt
    13201 aacaagtttt tttatattat aaaatttaaa aaattacatt ttatgcaaac ttttataaaa
    13261 acagttttta gcttgtcttt gtttttactt acacataatt ttttgttttc acaagtaact
    13321 gttcctatta gcgaaaaaga aatggaacaa tataatcgtg agcgtcaagc aattatggca
    13381 atgaaaatcg acttgtctca aacttggcta tatatggtta aaaacaatgt aatctcttca
    13441 aataaaattt tactttccga agttttttat tcaaaacaag gtttgcccga attagtggtg
    13501 caatacgatg aaaatcaaaa gattacatct agtacaagag taaaatataa ttctaaaaat
    13561 ttacctttcg aagaaataaa atttgcggca gactctagtt taataggtgg tattttgttt
    13621 gaatatgata aaaatggact tttgaacaag caagttcatt ataatgaaag tggtgttata
    13681 acagctattc aacaatattt aagaaaacaa gatacaatat ttatgtcagt ttttaataac
    13741 agtgctgagt ttatatatgg aaatgcctta atttttgaaa ataaaaatgg acaatcactg
    13801 ttaaagtcaa tgtataaaat tgattcaagc cacaatatta ttgaaaaaaa cttgttcgat
    13861 tatgatgaaa tatatgcttt aagaagcaaa atagtttatg atgacaaaga tttgggaact
    13921 cagaaaaatt ttgtttataa tgatgatgga gctttagtta gaacaataat tactaattct
    13981 gcaggaatac atattagcga cacttctttt gaatatgacg aatacggaaa tcttattaga
    14041 attgtagaat acgaagaaga gactggtaca gcaaaattgt tttttataaa ttatttaagt
    14101 ttagtaaaag aagaaaatgg aaataaaaac taaaatatat agacgacaac aacaagtaag
    14161 gtcttatgac attgatgaaa atctagaatt aaaattaaat gttatctttc agtggttcag
    14221 cgaaatagct tgggaacatg ctaaaatatt aggtataggt tttgaagaat taaaaaatgt
    14281 tgacaaattt tgggttttaa attctgtaaa tgttgaaatc aacaaacttc caaaatggca
    14341 agaccagctt attttagaaa cttggcccag cggaatttca ggcatacaat acactcgcga
    14401 atttcaattt tatgatgata aacaaaacgt attaattgca gcatcttcgt cttgggtaat
    14461 aattgataaa aacactttac gcccaatttt accaccagaa ttttcttact tagacgaact
    14521 tttaaaagaa aaagcaacag ataatttatt gaaaaaaatc cctttgcata aagatttgaa
    14581 atttgaaact gaagaaacag ctcgttacac cgatattgat atgcatcatc atgtaaacaa
    14641 tgcagtttat gtgaattgga tagaaaatat catgggaaat atcttaccaa acaaaattaa
    14701 acgttttaaa attcagtttt ctaacgagtt gaaattggga gataaagttt ctatttttac
    14761 aaaaaaagaa aataattaca tatattggga aagtttaaaa tctgatgata aagtttgttt
    14821 cagagcagaa gcagaatttg aaaataattt agaaattaat taaaaaattt gttttatgga
    14881 attaacagca cttgaaattt tgggatacat agcctcaatt cttattgctt ggtcgatggc
    14941 agtaagctcg attattaaat ttagatggat aaatttattt ggggcacttg gattaacaat
    15001 ttattctata ataatctctg caatccctgt ggcgattgtc aatgctttga ttgtaatttt
    15061 agacatttat tatctataca aagcatacac aaaaaatgaa gtttttcaaa cattagaatt
    15121 aagtgaagac gaccattatt taaaacagtt tattgagttt tataaaaacg acatcaagga
    15181 attttttcca aactttgaat ataaaccaga ttcgaaaaac ttacagttct tgattttacg
    15241 agacatgtat gttactggag tttttatggc aaaaaaaata gacgacaaaa cttataatgt
    15301 tgatttggac tatgtaagac ctgagtatcg tgattttaaa aatggaaaat atatttattc
    15361 acaaattaaa gaaaaatttt taatccaatc ttgcactaca gtcaaaacac aatccaaaaa
    15421 taaagcacat ataaaatatt tgaataaaat tggttttaaa aaagttggtg aaggagaatt
    15481 tgaaatgaaa ctaaaaaata attgataatt gataattaag aattaaggat tatttcaaat
    15541 tgaaacttcc ccgctcgctc ggatttgtaa tccgagtgta acataaccaa cactcagatt
    15601 acaaatccga gcgagcgaca aggatgctga gagagagcat tggtggggtt agaagcgagc
    15661 ggttgtgggt catctgtgag ggcaacacaa gaccgtcaaa ggcttgtccg taggacaaca
    15721 tgtgattaac cgtaggcgca gccaatacag ctaaaagttc ataaagaaag taaaaccgaa
    15781 cccgcaagct acttttaaca cttcaactac gttcagtgca tcgcttttaa cttttaactt
    15841 ttaactgatt tgccttgccc acaagggtaa cacaataaat ttattaaaag ttatttcaaa
    15901 ttgaaactta cgttaaggta tttatcactt ctgatatagg tattggttac atcataagaa
    15961 atagattgct gatctgcttc tgctgaaata agttgtgaag acataggata aagtgttatt
    16021 ttgcgactt
//
LOCUS       DAOPNX010000061        15503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_4528_length_15503_cov_6.715109, whole genome shotgun sequence.
ACCESSION   DAOPNX010000061 DAOPNX010000000
VERSION     DAOPNX010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15503
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4528_length_15503_cov_6.715109"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(312..3017)
                     /locus_tag="PKN53_07615"
     CDS             complement(312..3017)
                     /locus_tag="PKN53_07615"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HOD88851.1"
                     /translation="MIKSPLRYPGGKSRAVKTITKILILKSNAIKMRKFYFKNLKLSK
                     HIILYFLISVVLSSCSTKTKNFEYVTKSPDGKISMSFGIENGFLYYQVFSDNEMLIDK
                     SILGFEFKNAEEIKENFQIISSETKEFKEIWHPVVGENSEILNHYNESVFVLKESVSP
                     KRELIFSLRVYNDGIAFRYTILGNGENLEILNENTMFRFAKNDSAWWIPSNAFAYEAL
                     HKKSLLSEINEAATPITIEKENGKLLCIHEAALYNYSEMFLQNAGQNSPDFFVGLWPE
                     PDSVCARIKTPFSTPWRCVIIANSPAELFESNLILNLNEACKIEDVSWIKPLKFVGIW
                     WALHLGEQTWEGGEKHGATTENTKRYIDFAKKYKIGGVLAEGWNCGWDTWAKPNVIPK
                     QNFCVPESDFDLNEVVNYAKSNNVEFISHHETGGNIPDYEKQLDSALSLCNKLGIRYL
                     KTGYAGEIIPKDYPPYGQFMVNHFQKVVELAAKYKICLNVHESIKPTGIERTWPNLLT
                     QEVVRGNEWNAGYNATPPCHATILPFTRLVAGPMDYTPGIFKINHNPNENKRLYCTRT
                     YQMAMYVVFYSPMMMVSDKIENYENQKEFKFIEDVPCSWDKSKLIDAKLGEFVAVARK
                     KDENWFTGIITNQDTRLVKIPLNFLENDKFYLAEIYCDDFDTDWEKNPDKVEIASYFV
                     TNKDTVFAAVSKAGGHCMVLREIGAKDVLAGGIEKYNVSSVEKMKVFEKQNTYGRVVA
                     NNLAMQGKITLSQEFSKKYSASGKNALIDGLSGSYNMCENWQGFEGLEDLEIILDLGK
                     IQKFKSIKISFLNSPKSWIFSPKSVEFLISENGKNYISKNIVEIIETSEKEAHITNIQ
                     EVETENLEMEARYVKIKIITQKTCPDWHIGKGKKAWLFCDEVLVY"
     gene            complement(3014..4138)
                     /locus_tag="PKN53_07620"
     CDS             complement(3014..4138)
                     /locus_tag="PKN53_07620"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease, SacI family"
                     /protein_id="HOD88852.1"
                     /translation="MKETTILENILKKSTKKNVKINQTTSDNVRTLVKHIGSNKSLVS
                     ALVTSCLKKIIDPSQDIRLHRTDFNGGYSARSLDTNVTVPFFKLHFPKYANKESAFLT
                     LATRERIKWTIQEGEGLKIRNKEVKSAFLQLLDLVENKGKSEECLLSIFKELTLLSEK
                     ETLIFDETITALGFSNVLNINKVIDMLVTHFSLKLSSRLPVIAIYTIYQELIKNVKRY
                     NGKILKPLNVHTSADKHGYGDVEIWNKNNTPFEMVEIKHNIAIDRNLIFDVVKKSKNT
                     TIKRYYVLTTFTDSFLSKNEELYINNFILKIKQDTDIDVIANGIIPSLKYYLRFIDDY
                     KAFIKSYTKNLIADAKNSTEIKDFHLTTWQEILKKNNIDK"
     gene            complement(4135..5157)
                     /locus_tag="PKN53_07625"
     CDS             complement(4135..5157)
                     /locus_tag="PKN53_07625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOD88853.1"
                     /translation="MNFEKKYIDKVIQGDSLSILKEMPNDMVDLVVTSPPYFQQRDYG
                     NIGIGNEKTVEEYIRNLILIFEECVRVTKPTGSIVFNVGDKYINGNLLLVPYRFAIEA
                     QKNKDLRLVNELTWVKVNPTPKQDTRKLISSKEPFFIFAKSNDYYFDKNAFLEYKDMF
                     LNGNQTRNRKDVGKRYFELIENSNLSVEEKRQAKKELTEVILEVKSGKLESFRMKIRD
                     IHALPYGGQAGGRLTQIQNKGFTIIKIYGNSIRKDIIESTVETIKGNQHPAVYPEFVV
                     QELIKLLTPQKAIVLDPFLGSGTTAVVAKKLQRNYIGIEIYDKYVEYAKERINEISQP
                     QMQLFL"
     gene            complement(5276..6109)
                     /locus_tag="PKN53_07630"
     CDS             complement(5276..6109)
                     /locus_tag="PKN53_07630"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOD88854.1"
                     /translation="MIKSPLRYPGGKSRAVKTIAKLIPDFDEFREPFLGGGSVFIYAK
                     QRYPNKKFWVNDIYFELYKFWEMAQKDVNSLIDKIYEWREKFPVGKELHKFLNKNIET
                     FNDIERAAAFFIYNRITFSGTSLSGGFSEHAFAGRFTESSIQRLNKFAEVIKDSKITN
                     LDYEEVVNAKGKNVFVFLDPPYYSATKSALYGKNGNLHKGFDHERFANVMQKCSHKWL
                     ITYDDSKFIRELFSFANITTWNLTYGMRNVNENSDQNGKELFISNYNIYQPKAQQLTL
                     F"
     gene            complement(6179..9616)
                     /locus_tag="PKN53_07635"
     CDS             complement(6179..9616)
                     /locus_tag="PKN53_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOD88855.1"
                     /translation="MLFIFLSVITLNVFGQENHKSIHQLELEYYNSLNIDAESYYSIN
                     QPAEIDILKSSKSCEHTKIVFGWHPYWANGMETNYQWDLLTDMSFFSYEVNPSTGNAN
                     STHGWATSNAVTQALANGVRVNLCVTLFSEHATFLESSTARQTLITNLINLVQSRGAH
                     GVNIDFEGVPGAQKTNFTNFLIDLCNQMHSAIPNSKVSLCLYAVDWSNVFDIAAINNY
                     IDYYTIMGYDYYYGGSSYAGPTSPLYTFHTFNYNLAKSVNYYMSKGASKEKLILGLPY
                     YGKEWSTESQNVPSATTATGSSRTYKTIRANADGYYTNRQYENVSQCPYYVFYNSGWK
                     QCFVDDEESLGAKYDLVNMMGIGGIGIWALSYDDGYTELWNLIREKFTTCGSVPCNGI
                     FYDLGGPNNTYLNSSDYVFTIAPTGATKVNLQFTEFDIEAGSGSTCNYDYIEIFDGPD
                     ESSPSFGRFCNTTGNPGLISSTGNSLCVKVHTDGATVNNGFTGVWSCIQDDVLPTTEI
                     IAADWYKEDFTVNFVDEDNAGIDMQFYQVLDFDGNEWRANGNYGFFNDNFNTNIHSDW
                     INSSGIWTVNNGHLQQSDQTSANTNIYANVLQNNENIYLYHWQMKISGEGTNRRAGLY
                     VFCTDPGAIQRGDAYMVYFRADQNKCQLYRSTNNEIVIHTNDNVIVNADTWYDYKVMY
                     NPQTGELKAFQNNILVSSWTDPSPVSSGIAVSLRTGNCIVEYDDIKVYKSRSESALVS
                     VGANKEIRYENQDVNTSACRIKSVVTDINGNFSANSGKDINIDFSAPLQISKVNDGLA
                     EDIDVTYDNSQLQANWTEAVEENSYITNYEYCIGTNIGSNDVVDWTDNGLQTNVTHTG
                     LSLNSLNTYYFTVRAKNIVGLYSAEKSSDGVIFIDAQDNVIADFTISSAEICQGGQVQ
                     FINLSQNASTYLWEITGPQTLTSNLESPSFELQAGIYSVSLTAYGEYGEDTKTDEIEI
                     SVVPLPIADFEALNTLVQLPNAVVFFENNSQNADSWLWDFGDGTSSTNFEPWHEYENA
                     GVYTISLIAGNGVCEDNEMIKNNYITVEDVSSDKLMLAQEISIYPNPSIDKIFINSEN
                     EKINKLELYDVNSRLCLKLENLGNTLQLDISEFKDANYILVLFTNENIYKKKLVFVR"
     gene            complement(9751..10530)
                     /locus_tag="PKN53_07640"
     CDS             complement(9751..10530)
                     /locus_tag="PKN53_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88856.1"
                     /translation="MKKIILTSIILIFVLTQGYGQKQCDIKSNYEDFISVERGEYEGR
                     AYLTTQVVETQKKSCFSDLINNNPFLISYLLQFASVKNHKNILEITDSIDFKRAYFED
                     LRNDSLFNAIMVDLVSKTIDKTVPKDTVSFDEILNIAVKYFYIRKINEEGYYAAQICG
                     GLNAIEKTEAKREPFLEAFSFSSIQKNMLQGGDFNIYEEFINVVKELYLVNLGIDDNE
                     NLLRAQGATFMLMRNNENLRKMLKADYENQKEFLPFVVIEN"
     gene            complement(10697..11017)
                     /locus_tag="PKN53_07645"
     CDS             complement(10697..11017)
                     /locus_tag="PKN53_07645"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOD88857.1"
                     /translation="MKKSCCTLIFAVVAIFCFAQNGYKKVYKYGAYNRDWALVKDITG
                     NYGFIDSDGKEVVPPNYKKIGKFNKTVHNYALVKDVLGYYGLIDCYGKEVSAMHEDDS
                     EDEE"
     gene            complement(11078..12070)
                     /locus_tag="PKN53_07650"
     CDS             complement(11078..12070)
                     /locus_tag="PKN53_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOD88858.1"
                     /translation="MKTKFEILIALVLISFSFSTNLFAQRTTDIENGKDHPLISRFDN
                     AVIEFYKETKWGSYKLPVNEKGAIDWGNPNVLEGKVTRIQYTTSVDNNSEFVLHNYKA
                     AFKKAGFTVLISIANEELGVADRPHSWSDKYYRSGLYGNDGLHNDKFGIGLNFPSWKS
                     NHSFIVAQGNKAGKDIYAIIYTVVDDNYTLITQDVVEVEAVETGMVTAENISKGIQTE
                     GYFTVYDIFFDTEKSEVKPESADALKTIAEYLNANKTQKVIIVGHTDNVGDFDMNITL
                     SKDRANAVMEKLISDYAVSKDQLKPYGVGSVSPVASNSTEEGRAKNRRVEVVEQ"
     gene            complement(12235..13416)
                     /locus_tag="PKN53_07655"
     CDS             complement(12235..13416)
                     /locus_tag="PKN53_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOD88859.1"
                     /translation="MDTKNKGFNTKLIHAGDYEDAFGSAVMPIYQTSTFRFKSSKHGA
                     DCFSGKEAGYVYTRLGNPTVTALEEKIAQLENGYRAIAFSSGMSAVTAVYMTFLEKDA
                     HMISSSAIYGTSRAVLERDFSRFGVKCDFIDTSVISDIEKAIRPNTKLLYIETPTNPT
                     MVLTDIKAATELAHKHGIVVCVDNTFSSPYLQKPLDLGADVSLHSITKFINGHADIVG
                     GMLITKDKQIDEKLRTTMAYMGGNMDPHQAYMASRGVKTLSLRVEQSMKSAQKIAEYL
                     ENHPKVAWVKYPGLKSHPQHELAKKQMTGFGAMISFELKGGYAAGEKLMDNVHLAMLA
                     VSLGGVETLIQHPASMTHSSLSKESREAAGITDGLVRFSVGIENCEDIIADLENALAK
                     A"
     gene            complement(13485..14825)
                     /gene="mgtE"
                     /locus_tag="PKN53_07660"
     CDS             complement(13485..14825)
                     /gene="mgtE"
                     /locus_tag="PKN53_07660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOD88860.1"
                     /translation="MVEVKFDFQTFFDEKNWRGLKRALNEYDSIQIAEILEELEENQE
                     IVVFRLLSREQAKEVFQLLSQEKQEDIIDGIAHNVQNLSNLLNDIEPDDRTAFFEELP
                     GKVSQRLIQLLSPKERQITLQLLGYPEDSIGRLMTPEYVAIKAHYTVERAFQHIRKYG
                     RDSETLNVIYIIDDERKLIDDIKIKELILADPQEKVENLIDSKFVALNVMDDQETAIK
                     AFTDYDRVALPVVNDDGVLLGIVTFDDIMDVAEEESTEDFHKFGSIQSAITNPLKETI
                     FNLYKNRIIWLVALVFMNVFSGAAISHYESVIQTMVSLVFFLPLLIASAGNAGSQSAT
                     LMIRALAVGDVEVKDWYKLIWKELLVSLLLGLTMAATVALVASFRAPEIILVLIITMV
                     LIVITGSLVGLILPFIFTKFKLDPATASAPLITSIADICGILIYFSVAKWILGF"
     gene            complement(14978..>15503)
                     /locus_tag="PKN53_07665"
     CDS             complement(14978..>15503)
                     /locus_tag="PKN53_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88861.1"
                     /translation="VLILVFILLMKNASEYMGVYGDLDSTLAQASVVQQDLVRHEQYG
                     HNYYDIGKFEATPMGVLKKFPIATISGIYRPFLWEAGNPFILLAALEGTFLFLFMIYV
                     IFKTGFLRFFRIIFSDPFLIFSLSFVIIFGFGVGLATANFGALVRYKIPLLPFYVASL
                     FIIEYIYNKKKSLK"
BASE COUNT         5052 a         2714 c         2028 g         5709 t
ORIGIN      
        1 aataatttta tctaaaattg caataaatat ttttagttgc ggatttaagg aagtttttaa
       61 agtcggattt ataataaaat gtgtcaactt ttcttaggac gcgacatcag ctctttttaa
      121 cacttcaact acgttcagtg catcgctttt aaccttttaa cttttaactc aagtgtacgc
      181 tctttcgtcc tcaatttcag cgccttgcat tttaacactt ctgaaacacc ttgaaaattc
      241 attgttttaa aatttgtttt gagacagcct ctgctttgta taaaaatctt atcaaacttt
      301 aagttgtttt tctaatatac taaaacttca tcacaaaaca gccaagcttt tttccctttt
      361 ccaatatgcc aatctggaca agttttttgt gttattattt taatttttac atatcttgct
      421 tccatttcta agttttctgt ttcaacttct tggatattgg tgatatgtgc ttctttttca
      481 gaagtttcta ttatttccac aatatttttt gaaatataat ttttcccatt ttctgaaatt
      541 aaaaactcaa ctgattttgg tgaaaaaatc caacttttag gtgaatttaa aaatgaaatt
      601 ttaatgcttt taaatttttg aatcttgcct aaatctaaaa ttatttccaa gtcttctagt
      661 ccttcaaagc cttgccaatt ctcacacata ttataagaac ctgacagtcc atctatcaaa
      721 gcatttttac ccgaagctga atatttttta ctaaattctt gcgacaatgt tatttttcct
      781 tgcattgcta agttgtttgc aactactctt ccgtaagtgt tttgtttttc aaaaactttc
      841 attttttcta cagaactaac attatacttt tctattccac ctgccaaaac atcttttgct
      901 cctatttcgc gtaagaccat acaatgccca cctgcttttg aaacagcagc aaaaaccgta
      961 tctttatttg ttacaaaata actagcaatt tctactttat ctggattttt ttcccaatca
     1021 gtatcaaaat catcgcaata aatttcggct aaataaaatt tgtcgttttc taaaaaattt
     1081 aaaggaattt tcaccaatct tgtatcttgg tttgtaatta ttcccgtaaa ccaattttca
     1141 tctttttttc gagctacagc aacaaattcc ccaagttttg catctataag tttagattta
     1201 tcccaagagc aaggcacatc ttcaataaat ttaaactctt tttgattttc ataattttca
     1261 atcttatccg aaaccatcat cataggacta taaaaaacca catacattgc catttgataa
     1321 gttcgtgtac agtaaagtct tttattttca ttcggattat gatttatttt aaaaattcca
     1381 ggtgtgtaat ccattggacc tgcaacaagt cttgtaaaag gtaaaattgt tgcatgacaa
     1441 ggcggagttg cgttatagcc agcattccat tcattgcctc ttacaacttc ttgagttaac
     1501 aaattgggcc aagttcgctc tattcctgtt ggttttatgc tttcatgaac atttaaacat
     1561 attttatatt ttgctgccaa ctccacaact ttttgaaaat gatttaccat aaattgccca
     1621 taaggcggat aatcttttgg gataatctca cctgcataac ccgtttttaa ataacgaata
     1681 ccaagtttat tgcaaagact caaagcagaa tctaattgtt tttcataatc tggaatattt
     1741 ccgcctgttt cgtggtgaga aataaattct acattatttg attttgcata atttacaact
     1801 tcgttaaggt caaagtctga ctcaggaaca caaaaatttt gttttggaat aacatttggt
     1861 tttgcccagg tatcccagcc acaattccag ccttcagcaa gtacaccacc aattttatat
     1921 tttttagcaa aatcaatata tcgttttgtg ttttctgttg tagcaccatg tttttcacca
     1981 ccttcccatg tttgttctcc taaatgcaaa gcccaccaaa taccgacaaa tttcaatggc
     2041 tttatccacg aaacatcttc aattttgcaa gcctcgttta aattaagtat taaattagat
     2101 tcaaaaagtt ctgcaggcga attggcaatt attacacaac gccaaggagt agaaaatgga
     2161 gttttaattc ttgcacacac agaatctggt tctggccaaa gtccaacaaa aaaatcaggt
     2221 gaattttgac cggcattttg caaaaacatt tcagagtaat tatataaagc cgcttcgtga
     2281 atacaaagta atttgccatt ttctttttca atagtaattg gtgttgcagc ttcatttatt
     2341 tctgaaagga gtgatttttt atgcaaagct tcataagcaa atgcatttga tggaatccac
     2401 caagcagaat cgttttttgc aaatctaaac atggtgtttt catttaaaat ttccagattt
     2461 tcgccattac caagaatcgt gtaacgaaag gctatcccat cattataaac tcgtaaacta
     2521 aaaattaatt ctcgctttgg acttacactt tcttttaaaa caaaaacgct ttcattataa
     2581 tggtttaaaa tttctgaatt ttctccaact acaggatgcc aaatttcttt aaactccttt
     2641 gtttcactac ttataatctg aaaattttct tttatttctt ctgcattttt aaattcaaaa
     2701 cctaaaatag atttgtctat caacatttca ttatctgaaa aaacttggta ataaagaaat
     2761 ccattttcaa ttccaaatga catagaaatt ttaccatcag gcgattttgt tacatattca
     2821 aaattctttg tcttagttga gcaagaagat aaaacaacag aaataagaaa atataaaatt
     2881 atatgttttg acaattttag atttttaaaa taaaacttac gcatttttat cgcatttgac
     2941 tttaaaatta agatttttgt aatagttttt actgcacgac ttttgccacc tggataacgt
     3001 aatggggatt taatcattta tcaatattat ttttcttcaa aatctcttgc caagttgtta
     3061 aatggaaatc ttttatttct gtagaatttt ttgcatcagc aattagattt ttggtataag
     3121 attttataaa cgccttataa tcatcaataa aacggagata gtatttcaaa cttggtataa
     3181 tgccatttgc aataacatca atatcagtat cttgttttat ttttaaaata aagttattaa
     3241 tatacaactc ttcatttttt gataagaagc tatcggtaaa agttgtcaat acataataac
     3301 gttttattgt tgtatttttt gattttttca caacatcaaa aatcaaattt ctatcaatag
     3361 caatattgtg ttttatttca accatttcaa atggtgtatt atttttgttc catatttcca
     3421 catctccata gccatgttta tcagcggaag tatgaacatt cagaggtttt aatatcttac
     3481 cattgtatct ttttacgttt ttaattaatt cttgataaat agtgtaaata gcaataacag
     3541 gtaatcgtga gcttaatttt agagaaaaat gagttacaag catgtctatt accttattga
     3601 tattcaaaac attagaaaaa cccaatgcag taatagtttc atcgaatata agtgtctctt
     3661 tttctgataa caatgtaagc tccttgaata tagacaacaa acattcttca gacttccctt
     3721 tattttcaac caaatcaagc aactgtaaaa aagctgattt cacctcctta ttcctaattt
     3781 tcagtccttc tccttcttgt attgtccatt ttattctttc ccttgttgct aaagtcaaaa
     3841 aagcggattc tttatttgca tatttaggga aatgcaattt aaagaatggg acagtaacat
     3901 tcgtatctaa actgcgagct gaataaccac cattaaaatc tgttcgatgt aaacgtatat
     3961 cctgagaagg gtctattatt tttttcaaac aactcgtaac aagtgcagaa acaagagatt
     4021 tgttgctacc aatatgtttt acgagggttc gaacattatc agaagtagtc tgatttattt
     4081 tgacattttt cttagttgac ttttttaaaa tattttctaa aattgttgtt tctttcataa
     4141 aaataattgc atttgtggtt gcgatatttc gtttattctt tctttagcat attcaacata
     4201 tttatcgtaa atttcaattc cgatataatt acgttgcaat tttttagcaa ccacagctgt
     4261 agtacccgat cctaaaaatg ggtcaagtac aatagctttt tgaggtgtaa gtaattttat
     4321 taattcttgc acaacaaatt cgggataaac cgcagggtgt tgattgcctt taatagtttc
     4381 tacggttgat tcgataatgt cttttcgtat agaattacca taaatcttta ttatcgtaaa
     4441 gcctttattt tgtatctgtg tgagcctacc accagcttgc ccaccataag gcaatgcatg
     4501 aatatcgcgt attttcatgc gaaaactctc taatttacca gattttactt ctaaaataac
     4561 ttccgtaagt tctttttttg cttgtcgttt ttcttcgaca cttagatttg aattttcaat
     4621 caactcaaaa tatcttttgc caacatcttt tctatttctt gtttgattac cattcaaaaa
     4681 catgtcttta tactctaaaa atgcattttt atcaaaataa tagtcattgc tttttgcaaa
     4741 aataaaaaat ggttctttgg aagaaattaa ttttcttgtg tcttgcttag gtgttggatt
     4801 tacttttacc caagtaagtt cattcacaag tcttaaatcc ttattttttt gagcctcaat
     4861 agcaaaacga taagggacta ataacaaatt tccatttata tatttatctc caacattaaa
     4921 aacgatgctc ccagttggct tagtaactcg tacacattct tcaaatatta aaattaaatt
     4981 tctaatatat tcttcaactg ttttttcgtt accaattcca atattaccat agtcgcgttg
     5041 ttgaaaataa ggaggacttg taacaactaa atccaccatg tcgttaggca tttctttcaa
     5101 tatgctcaga gagtccccct gaattacttt atctatatat tttttttcaa aattcatatt
     5161 gcaaatataa tcaattttat gttatatttt caatagacag ttttcatttt agtagagata
     5221 ttatttgtgc tttattcatt taatgtgaga gttttagtaa atttacattc cttaattaaa
     5281 aaagtgttaa ttgttgtgct ttgggttgat aaatattata gtttgagata aaaagttcct
     5341 ttccattttg gtcagaattt tcgttaacat tcctcattcc ataagttaaa ttccaagtag
     5401 ttatgtttgc aaacgaaaaa agttctcgta taaatttact atcatcataa gttatcaacc
     5461 atttgtgtga acatttttgc attacgttag caaatctttc gtgatcaaag cctttgtgaa
     5521 gatttccgtt ttttccataa agagctgatt ttgtagcaga ataatatggt ggatcaagaa
     5581 atacaaatac gtttttccct tttgcgttaa caacctcttc ataatcaagg tttgtaattt
     5641 ttgaatcttt tataacttcg gcaaatttgt taagtctttg aatactactt tcggtaaaac
     5701 gtcctgcaaa tgcgtgttca ctaaaaccac cactcaaact tgttcctgaa aaagttatgc
     5761 gattataaat aaaaaatgct gcagctcttt caatgtcatt aaatgtttca atgtttttgt
     5821 ttagaaattt atgtaattct ttacccacag gaaatttttc acgccattca taaattttat
     5881 ctatcaacga attgacatct ttttgtgcca tttcccaaaa tttataaagt tcaaaataaa
     5941 tatcgtttac ccaaaatttt ttgtttggat atctttgttt ggcatatata aatactgacc
     6001 caccacccaa aaatggctca cgaaactcat caaaatcggg aatgagtttt gcaattgttt
     6061 ttactgcacg gcttttgcca cctggataac gtaatgggga tttaatcatt tcgagtatat
     6121 gagttttaca atgaaattat ttttattggc ttcaaacata attttatttt acaaatttct
     6181 atctcacaaa cactaatttt ttcttataaa tattttcgtt tgtaaataaa actaaaatat
     6241 aatttgcatc tttaaattca gaaatatcta attgtagtgt attgccaaga ttttcaagtt
     6301 tcaagcatag tcttgaatta acgtcgtata attctaattt atttattttt tcattttcgc
     6361 tatttataaa aatcttatca atacttgggt ttggataaat agaaatttct tgggcgagca
     6421 taagtttgtc agagctcaca tcttcaactg taatataatt gttttttatc atttcattat
     6481 cttcacaaac tccattgcct gcaatcaaac ttatagtata aacgcctgca ttttcgtatt
     6541 cgtgccaagg ttcaaaattt gtagaacttg ttccatctcc aaaatcccat aaccaagaat
     6601 ctgcattttg agaattattc tcaaaaaaca caacagcatt tggaagttga actaaagtat
     6661 ttaaagcttc aaaatctgct attggaagtg gaacaacact aatttctatt tcgtcagttt
     6721 ttgtatcttc accatattct ccataagctg taagagagac tgaataaatt ccagcttgta
     6781 attcaaaact tggcgattct aaattgcttg ttaaagtttg cggaccagta atttcccata
     6841 aatatgtgct tgcattttga gaaagattaa taaattgaac ttgccctcct tgacaaattt
     6901 ctgcagaaga aattgtaaaa tctgcaatta cattatcttg agcatcaata aaaataactc
     6961 catcgcttga tttttcagca gaataaagtc caacaatatt ttttgctctt actgtaaaat
     7021 aatatgtgtt caaagaattt aaacttagtc ctgtatgagt tacattagtt tgtaaaccgt
     7081 tatcagtcca atcaacaaca tcgtttgaac ctatatttgt acctatgcag tattcataat
     7141 ttgtgatata tgaattttcc tcaacagcct cagtccagtt tgcttgtaat tgggagttgt
     7201 cgtatgtaac gtcaatatct tcggctaagc cgtcattaac ttttgaaatt tgtaatggtg
     7261 cagaaaaatc gatatttata tcttttccag aatttgctga aaaatttcca tttatatctg
     7321 ttaccaccga ctttattcta catgcagaag tgttaacatc ttgattttca tatctaattt
     7381 ctttgtttgc tccaacacta actaaagccg attcgctacg tgatttataa actttaatat
     7441 catcatattc aacaatgcaa tttcctgttc gcaacgaaac tgctatccca gaactcactg
     7501 gcgatgggtc tgtccacgaa gacactaaaa tattattttg aaaagctttt agttctccag
     7561 tttgaggatt gtacataact ttataatcat accaagtatc tgcatttaca ataacattat
     7621 cattggtatg aataacaatt tcattgttgg tagaacgata taattgacat ttattttggt
     7681 cagccctaaa ataaaccata taagcatcgc cacgttgaat tgctccagga tctgtacaaa
     7741 aaacatacaa gccagctctt ctatttgttc cttctcctga tattttcatt tgccaatggt
     7801 ataaataaat gttttcatta ttttgcaaaa catttgcata aatattggta tttgccgatg
     7861 tctggtctga ttgctgtaaa tgtccattat ttacagtcca aatccctgat gaatttatcc
     7921 aatctgaatg aatatttgta ttaaaattat cattaaagaa accatagttt ccatttgctc
     7981 gccactcatt tccgtcaaaa tcaagcactt gataaaattg catatcaatt cctgcattat
     8041 cctcatcaac aaaatttaca gtaaaatctt ctttatacca atctgctgca attatttccg
     8101 tagttggcaa aacatcatct tgaatacaag accaaactcc agtaaatccg ttgttcacag
     8161 tagctccgtc agtatgcact tttacgcata aagaatttcc ggtagaacta attaatccag
     8221 gatttcctgt agtattacaa aatcttccaa aacttggcga gctttcatct ggtccatcaa
     8281 aaatttcgat ataatcgtaa ttacaagtag aaccactacc tgcttcaata tcaaattctg
     8341 taaactgtaa atttactttt gttgctcctg ttggtgctat tgtaaaaaca taatcgcttg
     8401 aattaagata agtattattt ggtccaccca aatcgtaaaa aatgccattg caaggtacgc
     8461 ttccgcaagt tgtaaatttt tcgcgaataa gattccaaag ttcagtatag ccatcgtcat
     8521 aacttaatgc ccaaattcca attccgccaa ttcccatcat atttactaaa tcgtattttg
     8581 cccccaaact ttcctcatca tcaacaaaac actgtttcca accagaatta taaaaaacat
     8641 aatatggaca ctggctaaca ttttcatatt gacgatttgt ataatagcca tcggcatttg
     8701 ctctaatagt tttataagtc cttgaagagc ctgttgcagt agttgctgat ggaacatttt
     8761 gactttcagt actccattct tttccgtaat atggtaatcc taaaatcaat ttttctttgc
     8821 ttgctccctt tgacatataa taatttactg attttgcaag attataatta aaagtatgga
     8881 aagtatataa aggcgatgtt ggaccagcat aactacttcc accataataa taatcataac
     8941 ccataattgt atagtaatca atataattat ttatggcagc aatatcaaaa acattagacc
     9001 aatctactgc atacaaacaa aggctaacct tagaatttgg aattgctgaa tgcatttgat
     9061 tacataggtc aattaaaaag ttagtaaaat ttgttttttg agcaccaggc actccttcaa
     9121 aatcaatatt tacaccatga gcaccacgag actgtacaag atttatcaaa tttgtaatta
     9181 aagtttgacg tgcagtagat gattctaaaa atgttgcatg ctcagaaaat aaagtaacac
     9241 ataaatttac tcttacacca tttgctaaag cttgagttac agcatttgaa gttgcccaac
     9301 catgagtaga gtttgcattt cctgttgaag gatttacttc ataagaaaaa aatgacatgt
     9361 cagtcaataa atcccattga tagttcgttt ccataccatt tgcccaataa ggatgccaac
     9421 caaaaacaat tttagtatgc tcacaagatt ttgatgattt caaaatatca atttcagccg
     9481 gctgatttat tgaataataa gactccgcat caatatttag tgagttataa tattctaact
     9541 caagttggtg aatagatttg tgattttctt gaccaaaaac attaagtgta ataacactta
     9601 aaaaaataaa aagcaatgat aaatttttca taacaacatt aaaactaatt caaaatttcg
     9661 ctaaaaaaac ttagtaaata taaataaaaa atacgtattt tagtaaaaga agtttcgttt
     9721 ttggaatttt tttaaagttt ccaacagcta ttaattttct attactacaa aaggtaaaaa
     9781 ctctttttga ttttcgtaat ctgctttcaa catttttcta aggttttcat tattcctcat
     9841 gagcataaac gttgcccctt gagctcttaa taaattttca ttatcgtcta ttccaagatt
     9901 tactaaatac aactctttta ctacatttat aaattcttca taaatattaa aatctccacc
     9961 ttgtagcata tttttctgaa tagatgaaaa tgaaaaagcc tctaaaaatg gttctctttt
    10021 tgcttcggtt ttttcaattg cgtttaaacc accacaaatt tgagcagcat aatatccctc
    10081 ttcatttatt ttccttatgt aaaaatattt tacagctata ttcaatattt cgtcgaaaga
    10141 aactgtgtct tttgggactg tcttatcaat tgttttgctt actaaatcta ccataatagc
    10201 attaaaaaga ctgtcatttc ttagatcttc gaaataagcc cttttaaaat ctatactatc
    10261 tgttatttcc aaaatatttt tgtgattttt tactgaagca aattgtaata aataactaat
    10321 tagaaaagga ttattattta tcaagtctga aaaacaactt ttcttctgtg tttcaacaac
    10381 ttgtgtcgtc aaataagctc ttccctcata ttcacctctt tcaaccgaaa taaaatcttc
    10441 atagttagat ttaatatcgc attgtttttg tccataccct tgagttagaa caaaaattaa
    10501 gattatagat gttaaaatta tttttttcat aactcagcct tgtttttttt gattaataaa
    10561 atctattact atataattct ttgtgatttt tcaatctcaa agataataat taatttttat
    10621 tttaagaaat ttgcagtatt attagaaaaa caagccagat attatacatt aacttatact
    10681 cttgtaaacc tttaagctat tcttcatctt cactgtcatc ttcatgcata gctgatactt
    10741 ctttaccata acagtcaata agaccataat aacccaagac atctttgact aaagcatagt
    10801 tatggacagt tttattaaat tttccaattt ttttgtaatt gggaggaact acttctttgc
    10861 catcactgtc aataaatcca taattcccag taatatcttt taccaatgcc caatctctgt
    10921 tgtatgcacc atatttatac acttttttat atccattttg tgcaaaacaa aaaattgcaa
    10981 ctacagcaaa tattaatgta caacaagatt ttttcatttt attttaattt catcattaca
    11041 aaaattgtta ttatctatac cgcttgtcgc atattaatta ttgttcaact acctcaacac
    11101 gtctgttttt agctctaccc tcttcagttg aattgcttgc aacaggagaa acagacccca
    11161 caccatatgg tttgagttga tctttagaga cagcataatc agatataagt ttttccatta
    11221 ctgcatttgc tcggtctttt gaaagagtta tattcatatc aaaatcacct acattatctg
    11281 tatgtcccac tattataact ttttgggttt tatttgcatt caaatattcg gcgatagttt
    11341 tcaaggcatc agcagattct ggttttactt cagatttttc tgtatcaaaa aagatgtcat
    11401 aaacagtaaa atatccttca gtttgaattc ctttcgaaat attttcagca gtaaccattc
    11461 ctgtttcaac tgcttcaact tcaacaacat cttgagtaat taaagtgtaa ttatcatcaa
    11521 ccacagtata aataattgca taaatatctt ttcctgcctt atttccctgt gcgactataa
    11581 acgaatgatt gcttttccaa gacgggaaat tcaatccgat tccaaattta tcattatgta
    11641 agccatcgtt cccatataat cctgaacggt agtatttgtc gctccatgaa tgaggtctat
    11701 cagctacacc aagctcttca tttgctatag agattaaaac cgtaaaacct gcttttttaa
    11761 atgctgcttt gtaattatgt aaaacaaatt ccgagttatt atcaacagag gtagtgtatt
    11821 gaatacgtgt aaccttgcct tctaaaacat taggatttcc ccaatcaata gctccttttt
    11881 cgttaacagg taatttataa gatccccatt ttgtttcttt ataaaactca attacggcat
    11941 tgtcaaaacg actaattaac ggatggtcct taccattttc aatatcagtt gtgcgttgag
    12001 caaataaatt tgttgaaaat gaaaaactga taagaacaag tgcaattaaa atttcaaatt
    12061 ttgttttcat aatttttcgc tttaattttt aaaatttatt tataaagaca aatgtaaaca
    12121 tattttttta tttaaaaaaa aatatttttc ttctacttat ttttaataaa agcgaaagca
    12181 aggcaaacaa agctgccttg ctttcaattt ttatgtatat gaatgtgtta actactaagc
    12241 ttttgctagt gcattttcta ggtctgcaat aatatcttcg caattttcta tacctactga
    12301 gaatctaact aaaccgtcag taattcctgc tgcttctctt gattctttag ataagctaga
    12361 atgcgtcata gaagctgggt gttgaattag agtttctact ccgcctaaag aaactgctaa
    12421 cattgctaag tgtacattat ccataagttt ttcgcctgct gcatatccac cttttagttc
    12481 aaaactaatc attgctccaa atcctgtcat ttgttttttt gctaattcgt gttgtgggtg
    12541 agattttagt cctggatatt ttacccatgc tactttagga tgattttcta aatattcagc
    12601 tattttttga gctgatttca ttgattgttc tactctcaaa gataaagttt ttactccacg
    12661 acttgccata tatgcttggt gaggatccat atttcctccc atataagcca ttgttgttct
    12721 caacttttca tctatttgct tgtcttttgt aattaacatt cctccaacaa tatcagcatg
    12781 accatttatg aattttgtaa ttgaatgtag agaaacatca gcacctaagt ctaaaggttt
    12841 ttgcaaatat ggtgatgaaa aagtattatc aacacaaaca acaattccgt gtttatgagc
    12901 aagttctgta gctgctttta tgtcagtcaa aaccattgtt ggatttgttg gagtttcgat
    12961 atacaacaat ttagtatttg gtcgtattgc tttttcgata tctgatatta ctgaagtatc
    13021 gataaaatca cattttacac caaagcgaga aaaatctctt tctagaactg ctctcgaagt
    13081 gccgtaaatt gcacttgagc taatcatatg tgcatctttc tctaaaaaag tcatatacac
    13141 tgctgtaacc gcagacatac cacttgagaa agctatagca cgataaccgt tttctagttg
    13201 agctattttt tcttctaaag cagtaactgt tgggtttccc aaccgagtgt aaacataacc
    13261 tgcttctttg cccgaaaagc aatctgcacc gtgttttgaa cttttaaatc taaatgttga
    13321 agtttgataa attggcatta cagctgagcc aaaagcatct tcataatcac cagcgtgaat
    13381 taatttggtg ttaaaacctt tgtttttagt atccattttg taaaattttt atattattat
    13441 tttaaaacta tattttatta aatcacaaaa ttaacaatat ttttttaaaa tcctaatatc
    13501 cattttgcta ctgaaaaata aattaatatt ccacaaatat cagcaataga tgtaatcaat
    13561 ggtgcagaag ctgttgcggg gtctaattta aattttgtaa aaataaaagg taatatcaat
    13621 cccactaaac ttcctgttat tacaattagc accattgtta tgattaagac taaaattatt
    13681 tctggtgctc taaaacttgc taccaaagct acggttgcag ccatagtaag acctaaaagc
    13741 agtgatacaa gaagctcttt ccaaattaat ttataccagt ctttaacttc tacatcgcct
    13801 accgctaaag ctctaatcat aagtgttgcc gattgcgaac ctgcatttcc agcactagct
    13861 atcaaaagtg gaagaaaaaa cactaaagaa accattgttt ggataacact ttcgtaatgt
    13921 gatattgctg cacctgaaaa aacattcata aaaactaatg caacaagcca aattatacga
    13981 tttttgtaaa gattaaatat tgtttctttc aaaggattag taattgcaga ttgtatagaa
    14041 ccgaatttgt ggaagtcttc ggtagattct tcttctgcaa cgtccataat gtcgtcgaaa
    14101 gtaacaattc caagcaatac tccatcgtcg tttacaactg gtaaagccac tctgtcgtaa
    14161 tctgtaaaag ctttaattgc tgtttcttgg tcgtccatca cattaagagc aacaaactta
    14221 gagtcaatta aattttcaac tttttcttga ggatcggcaa gtattaattc tttaattttt
    14281 atatcgtcaa ttaattttct ttcatcatca ataatataaa ttacgtttaa tgtttctgaa
    14341 tcacgtccat attttctaat atgctggaat gctctttcaa cagtataatg tgctttaata
    14401 gctacatatt ctggtgtcat caaacgtcca atactatctt cgggatagcc aagaagttga
    14461 agtgtaattt gtctttcttt aggcgataaa agttgaatta atctttgaga aacttttcct
    14521 ggaagttctt caaaaaaagc agttctatcg tcaggttcaa tatcatttaa taagttggaa
    14581 agattttgaa cattatgtgc aataccgtca attatgtctt cttgtttttc ttgtgataaa
    14641 agttggaaaa cttcttttgc ctgctctcga cttaaaagcc taaaaacaac aatctcttga
    14701 ttttcttcta gttcctcaag gatttctgca atttgaatac tatcatactc attcaaggct
    14761 cgttttaagc ctctccagtt tttttcatca aaaaaagttt gaaaatcaaa ttttacttca
    14821 accatttttt tgcctcctca cttttgctct gtaaggggca taaagacacc ttacagatgt
    14881 gttaataatt attcttacta tgataagggc cttcgtccat ttgtaaaaaa ttttgtttag
    14941 cgtgcaaaag taaaaaaata tttagaaaat gaaaaaatta ttttaggctt ttctttttat
    15001 tatatatata ttcaattata aacaaacttg ccacgtagaa tggtaaaaga ggaattttgt
    15061 atctaactaa agctccaaaa ttagcagtag ctagtccaac accaaaacca aatattatta
    15121 caaaactcaa agaaaatatt aaaaatggat ctgaaaaaat aattctgaaa aatcttaaaa
    15181 aacctgtctt aaaaataaca taaatcataa ataaaaataa aaaagttcct tctaaagctg
    15241 caagtaaaat aaaaggatta ccagcttccc ataaaaaagg tctataaatt ccagaaattg
    15301 ttgcgatagg aaactttttt aacaccccca tgggagttgc ttcaaatttt ccaatatcat
    15361 aataattatg tccatattgt tcatgacgta caaggtcttg ttgtacaacg cttgcttgag
    15421 ctaaagtgct atctaaatct ccgtaaactc ccatatattc agaagcattt ttcattaata
    15481 gtataaaaac taaaatcaaa aca
//
LOCUS       DAOPNX010000062        15392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_4573_length_15392_cov_6.912695, whole genome shotgun sequence.
ACCESSION   DAOPNX010000062 DAOPNX010000000
VERSION     DAOPNX010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15392
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4573_length_15392_cov_6.912695"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            300..1457
                     /locus_tag="PKN53_07670"
     CDS             300..1457
                     /locus_tag="PKN53_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD88862.1"
                     /translation="MAKILIIDDERAIRNTLKDILSFEKHNVDLAEDGVEGIKLTEDN
                     TYDLIFCDIKMPQMDGIEVLEVLHETIPETPVVMISGHGTIDTAVEAIKKGAFDFISK
                     PLDLNRIMITLRNALDKSNLIMETKTLKKKVDGKYQMIGESEAINKVKDMIERVAPTD
                     ARVLITGENGTGKEVVARWLHEKSNRAQAPFVDVNCAAIPSELIESELFGHEKGAFTS
                     AIKQRKGKFEQADGGTIFLDEIGDMSLSAQAKVLRVLQENKITRVGSDKDIDVNVRVI
                     AATNKNLAEEIEKANFREDLYHRLSVILIHVPSLNEREGDIPLLADHFIEISCQEQGL
                     AVKKITKEALKELEKINWTGNIREFRNVIERLVILCGPVIDAADVITYAGK"
     gene            1525..2751
                     /locus_tag="PKN53_07675"
     CDS             1525..2751
                     /locus_tag="PKN53_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD88863.1"
                     /translation="MNLQAIKQRFGIIGNSKGLNRALDIAVQVAPTDLSVLVTGESGV
                     GKEKIPQIIHAYSRRKHNNYIAVNCGAIPEGTIDSELFGHVKGAFTSAHSDRKGYFEE
                     ANKGTIFLDEVAELPLATQVRLLRVLEIGEFIKVGSSKPEKTDVRVVAATNVDLQQRV
                     REGKFREDLYYRLNTVPIVVPPLRHRQDDIHLLFRKFASDFSEKYNMPAIRLSEDAVH
                     VLNSYRWPGNIRQLKNITEQISIIEEERIISAELLKSYLEGAETSNLPVLSKKGNYEN
                     FASEREILYQVLFDMKRDMNELKVLVNDIIQQNPNVKITNYPNNTFNNFDNEVQINPI
                     NHVYSNEQPVIHHHEPHQEHGEVVEEVLSLAEQEKELIIKALEKHNNKRKLAAEELGI
                     SERTLYRKIKEYQINK"
     gene            2752..3267
                     /locus_tag="PKN53_07680"
     CDS             2752..3267
                     /locus_tag="PKN53_07680"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOD88864.1"
                     /translation="MKNIRNIVYFLIFISSIALCSCTGGYSFTGADISADIKTIQVNF
                     FPNQASMVQPNLSTVFTETLKDKFISQTNLEMVNYDGDIAFEGAIINYNVTAQSFQGN
                     ETAALNRLTITVKVKFTNTKETAKSFEANFTRYADFESSINLASIEGELIQSISEELV
                     TDIFNRAVANW"
     gene            3271..4614
                     /locus_tag="PKN53_07685"
     CDS             3271..4614
                     /locus_tag="PKN53_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88865.1"
                     /translation="MNKEQFLKYCSDFSLLDSKSLEDISALIEEFPYFQTARILYAKN
                     LNSLKDVRFENKLRNTVVYVPDRKHLVRLINLKNLPENEITFIDEPKLVDQDIQIEET
                     NQEEPAEIQPVNQEIEVKQEEIIETPQVSQEVETKEVKQEEIADKQQTVQAVEIEETK
                     QEEVVEIHDISSEIKEEEVTENILPEFTNDVQTVDEAPNDEKLVEKEPTQQEDYSEDL
                     SETDRLKSIIEARLRELGINEKVRIGNEETEEIKQESTVNQQVNQKNQVEEIEEDVVI
                     NQQISEEIKEEASENIFIDFSDDAKKIDESINKNNGFELLDFDFEIEKDAILEHQNKK
                     TDAKDYDILENFSQKTTNNKPEKINLIDKFLAENPRIIPDKEYVSDNSLASKSLHIDD
                     SDLFSETLAKIYIKQEHFEKAILTYEKLCLKYPEKSIYFVGQIEKIKELIKNKTN"
     gene            4617..4940
                     /gene="secG"
                     /locus_tag="PKN53_07690"
     CDS             4617..4940
                     /gene="secG"
                     /locus_tag="PKN53_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOD88866.1"
                     /translation="MYIFFSVLIVICAVLLILIVLVQNSKGGGLASNFGQGNQVMGVK
                     KTADFLEKATWGLAIGLIVFSLLASFSIPNNQKIKKQDFEQTQTAPAESSEPASGAGE
                     PLPVE"
     gene            complement(4983..6170)
                     /locus_tag="PKN53_07695"
     CDS             complement(4983..6170)
                     /locus_tag="PKN53_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOD88867.1"
                     /translation="MRTICFYFQVHQPFRLKRYKFFDIGNDHYYYNDFENRNIMRKVA
                     EKCYLPTNQVFLDLIQEYGDRFKIAYSISGVAIDQFEMYAPDVLESFKRLVATGNVEI
                     LSETYAHSLSALKSYEEFERQINMHRQKIKEHFGVEPKIFRNTELIYSDQIGEYISNM
                     GFKGMITEGAKHILGWKSPNYLYCNAINPKLKLLLKNFKLSDDIAFRFSNHAWEEFPL
                     TTEKFVSWLNAIDPKEEMVNLFMDYETFGEHQWEETGIFEFLKALPDAVFRYSNYSFS
                     TPSEIVENAEPVAKIHVPYPISWADEERDLTAWLGNELQDEAFNNLYSLYDKVKEIND
                     PEIWKDWLYLQTSDHFYYMCTKWFSDGDVHKYFNPYESPYEAFVNYMNILSDFIIRLK
                     KYK"
     gene            complement(6243..7529)
                     /locus_tag="PKN53_07700"
     CDS             complement(6243..7529)
                     /locus_tag="PKN53_07700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOD88868.1"
                     /translation="MRVLMFGWEFPPHITGGLGTACYGLTRGLSHFGTEVIFVVPKSY
                     GDEDKTYINLVDAGQVDITERTTKETKFWKNFRYIEVGANLIPYMDPEEFKKIFAGKS
                     LSHSEFKESVLSKNFKFSGSYGASLYEEVARYALIAAYIASTHQFDVIHAHDWLTYHA
                     GIAAKKVSGKPLVVHMHATEFDRSGENVNQRVYDIEREGMEAADRVIAVSNLTRRTII
                     ERYGIHPDKVVTVHNSVEPITLPDEFRVTKGFDEKIVTFLGRVTFQKGPEYFIEAAKK
                     VLDKDPNVRFVMAGSGDMLNRMIKRVAKLNIATKFHFAGFLRGQEVYDMFSISDVYVM
                     PSVSEPFGISPLEAMMSDVPVIISKQSGVAEVLKHALKVDFWDVDDLADAIYGMLHYP
                     ALSRMFRKYGKIEVENLRWENSAFKVNKVYNDLIKN"
     gene            complement(7526..9481)
                     /locus_tag="PKN53_07705"
     CDS             complement(7526..9481)
                     /locus_tag="PKN53_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOD88869.1"
                     /translation="MNYIEFDKEQLINLEYSLKRELLRTNRAGSYASTTIVGCNTRKY
                     HGLLVCPMEDIDNDNHVLLSGIDISVIQNDAIFNFGVRKYPGNNYNPKGHKYVRDFYI
                     DVVPKLVWRVGGVVLQMERILTEDDRIIIKYTLLEAVSKTKIRFKPFLAFRNVHKLSK
                     ANLFVNTKFTPVNNGIKLRMYDAYKFLYLQTNKEAEYIHAPDWYYNVEYEEEQRRGYD
                     YQEDLYVPGYFEADIKTGETIIFTAGLDEANPKNLARKYNAEIAKRMPRTNYRECLES
                     AAKQFIYKRKGKSEIVAGYPWFGRWGRDTFISLPGLTLYQNDIKTCKEVLDTMTSEIK
                     DGLFPNIGAGNNAALNSVDAPLWFFWALQEYLKFTKDKVKIWKDFGEKIKNILENFRS
                     GTQFNIKMHDNGLIWAGEAGKALTWMDAVVAGKPVTPRHGFAVEINALWYNAVKFSLE
                     LAEHNKDKDFIEAWKHVPEITKSSFLQFFWDDNKHYLADYCTYEYKDWAIRPNQIFAC
                     SLPYSPLSEEKRERVLEKVKKELLTPKGLRTLSPSSPDYKGVYKGNQEQRDNAYHQGT
                     AWPWLLGSFCEGWLKIHGESGLGLVEKIYLNFEEDMTIAGIGNISEIYDGDPPHNARG
                     AIAQAWSVAELLRIANMIDNYKNNIKS"
     gene            9774..10340
                     /gene="lpcA"
                     /locus_tag="PKN53_07710"
     CDS             9774..10340
                     /gene="lpcA"
                     /locus_tag="PKN53_07710"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009161045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HOD88870.1"
                     /translation="MIRNNFLESSKLLNEFISDNKNFETIESAAKLMIKSINNGGKII
                     SCGNGGSMSDAMHFAEELSGRFKEERKALPAIAIADPSHITCVANDYGFDFIFSRFIE
                     ALGNKNDVLLAISTSGNSKNVINAVNSAINIGMPVVALTGKDGGKLKNLCDIEIRVPH
                     FGYSDRIQEIHIKVIHSIIDYIETNIDK"
     gene            10350..11699
                     /locus_tag="PKN53_07715"
     CDS             10350..11699
                     /locus_tag="PKN53_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOD88871.1"
                     /translation="MTIHFIAIGGAVMHNLAIALHKKGYQVSGSDDEIFEPSFSRLKK
                     HGLLPKKFGWFPEKINADLDIIILGMHARKDNPELLRAQELNLKIYSFPEFLYEQTKD
                     KKRVVIGGSHGKTTITSMIMHVLRDAKLNFDYMVGANIRGFDTMVNLEEKNKVAVFEG
                     DEYLSSPLDLTPKFHWYKPDIAVLTGIAWDHINVFPSFENYVEQFDIFIKHISKNGKF
                     FWFADDEHLQKLVKNNNVDNKAYLAFEHKVSEDGKTLVKFDGKTYTMSVFGNHNMQNI
                     NAARLVCLELGIKEKFFWESIQTFEGASKRLQLIKETENSSFYLDFAHAPSKLKATIN
                     AIKNKNPDRKLIACIELHTFSSLQHNFIPQYKNTMDEADIAIVYYSHEVLEHKRLPAL
                     PEDYVKESFGGKVQIFTDAELLKDYLAKQNYDNTNVLMMSSGNFNGIDFVDFANKLI"
     gene            11701..12159
                     /locus_tag="PKN53_07720"
     CDS             11701..12159
                     /locus_tag="PKN53_07720"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HOD88872.1"
                     /translation="MKKYFFSLIIVSILVSSCSFNKLHIGQPESLEVEELSMQGVRLK
                     VYVPIENPNNLSFNVKNLNLDLILNDRNVGKIKKLKKTKILKNSKQTYPFSFEVTPKD
                     ALTNIIYLVSELQRRNPELKIDGSVTVSKMLISKKIKIDHKESFDKYYKK"
     gene            12185..12625
                     /locus_tag="PKN53_07725"
     CDS             12185..12625
                     /locus_tag="PKN53_07725"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOD88873.1"
                     /translation="MSRFLSIILLFLSIGIVAKSQNITPMSISYFNSSAYNGKVKIKQ
                     DAKLKNFVETSVGLNKKRNGFYGYRVKIYAENHPNARSQANTVRTGFRQDGEKAYLNF
                     IEPNFEVLVGDFTNRFDAVKLLKKINTKYPDAYVVKTIVSYPNH"
     gene            complement(12629..14818)
                     /locus_tag="PKN53_07730"
     CDS             complement(12629..14818)
                     /locus_tag="PKN53_07730"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOD88874.1"
                     /translation="METQIPKHKMLKYTTFLKSCLPPIFFEIMDEEDFVTHYKLIKRT
                     IAPEGKDKLFHMKGDIEEFYSHSFCYDTLKETLLKEAAEVVENIAIYRVFPNQKEDFI
                     LSSTYRSLESSVRLEEFELPSEEKIRQSFYDEKGKRIKDIEVHLEDDARKVVVQCMNI
                     AFNTLLSNLFESIEKQAIKYSFLSIIQLAKPLNRNNKYYKVEIHLDEQISEEDFHSLK
                     EDFERYIQTWMQALSIFDMIGPAMVGPSSSHTAGANRIGQISRNIILAIIERGEVIKD
                     VELKFYSSFRDTGVGHRTPNALAGGLYGFATDHPEMIEHGDPIFLKENGIKFGEQTAK
                     FKGFVKASPSEDLKYLSQRNNNIAEVIFSTDKDTYSIVGFSIGAGNVEIRFFNDHELQ
                     TPIDGKSDLRLIGNDIVSSKDENYPIIRKIYNEQTESEKPRLEFNTFEELEEYLERTG
                     KNIIDVICETEFALQGMKREDVYSKMQEYWEIMKDSVYSGISTSEQSLLKLSGGDSKK
                     IVSYLPQSKLFNNVYGRAAAYATAVNEINAKSGVIIACPTAGSCGILPGVLTAWMEDN
                     PNSEKKILESLMVAGFFGMILFTDVSTAGADYGCQAEVGAAAAMAASAIAYLENGNHA
                     QLIQAFTIAIKNCLGLICDPVAGLVEVPCVKRNGIYSSLALSAALMALSGVQSFISPD
                     EVMLTMREVGERLHIDYKETGRGGLARTRDGKEVDKRFAQEVKKFFG"
     gene            complement(14845..>15392)
                     /locus_tag="PKN53_07735"
     CDS             complement(14845..>15392)
                     /locus_tag="PKN53_07735"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOD88875.1"
                     /translation="ISIGLSMDSLAISIATACSCENKTKITNLRFALILAFVQAICIV
                     VGWLAGSSLTKMFQQYDHWIAFALLTIIGGKMLIDGIRKKEDKAEINMNNFFVVAGLG
                     VATSIDAVVVGVSLPFVGINIWLTALIVFFTTFILSYLGLISGNFLRKKFKRLPVEII
                     GGLFLIGIGIKILLEHLINGC"
BASE COUNT         5357 a         2482 c         2295 g         5258 t
ORIGIN      
        1 acaagcaaag tatttttaga tttttcatgt aagtaatttt taaaatttaa cctttataaa
       61 acttattatt gcctgtcctt tagcaagcat ttaggacaaa aaaaccaact tcataaaagc
      121 tttagcttaa tattctacaa taataagcaa aatatcttaa atagcaatgc tttttcaata
      181 attttgtggt ataaatttta gtttaatttg attagccttg tagtttttaa actatttaaa
      241 aattttaata gtagttttaa aaaaataatt ttgttaaatt tgcacaaaat tctaaaaaaa
      301 tggcaaaaat tttaataatt gatgacgaaa gagctatccg caacacttta aaagatattt
      361 taagttttga aaaacataat gttgatttag ctgaagatgg tgttgaaggg ataaaattaa
      421 cagaagacaa tacttacgat ttaatttttt gtgatattaa aatgcctcaa atggacggca
      481 ttgaagtttt agaagttttg catgaaacaa ttcccgaaac tcctgtggtt atgatttctg
      541 gtcatggcac tattgatact gctgttgaag caattaaaaa aggtgctttc gactttatta
      601 gcaagccatt ggatttgaac cgaataatga ttactttgag aaatgctctc gataaatcaa
      661 acttgataat ggaaactaaa actttgaaga aaaaagttga tggaaaatat caaatgattg
      721 gtgaatctga agctattaac aaagttaaag acatgattga acgcgttgca ccaaccgatg
      781 ctagagtttt aattactggc gaaaacggaa ctggtaaaga agtagttgct agatggcttc
      841 acgaaaaaag caatagagcg caagctcctt ttgttgatgt taactgtgca gcaattccat
      901 cagaacttat tgaaagtgag ttgtttggac atgaaaaagg agcattcact tcggctataa
      961 aacaaagaaa aggaaaattt gaacaagcag acggcggaac aatttttctt gacgaaattg
     1021 gagatatgtc attgtcggct caagcaaaag ttttaagagt tttacaagaa aataaaatta
     1081 cacgagtagg ctccgacaaa gatatagacg tgaatgtgag agtaattgct gctacaaata
     1141 aaaatcttgc agaagaaatt gaaaaagcaa attttagaga agacctttat catagattaa
     1201 gcgtaatttt gattcacgtt ccttcactaa acgaaagaga aggtgacatt cctcttttag
     1261 ctgatcactt tatagaaatt tcatgccaag aacaaggctt agctgtcaaa aaaattacta
     1321 aagaagcatt aaaagaatta gaaaaaataa actggacagg aaatatacgt gagttcagga
     1381 atgtaattga aaggcttgta attctatgcg gtccagtaat cgacgcagca gatgttataa
     1441 cttatgctgg aaaataattt tctctgacat tttgtcataa aataattttg cttatatttg
     1501 tatcttctta aaatttcgta attaatgaat ttgcaggcta taaaacaaag atttgggatt
     1561 attggaaatt ctaaaggatt aaatcgagct ttagacattg ctgttcaagt agctccaacc
     1621 gatttgtcgg ttttggtaac tggcgaaagt ggagttggaa aagaaaagat tccgcaaata
     1681 attcatgctt atagtagaag aaaacacaat aattatatag ctgttaactg tggagcaatt
     1741 cccgaaggca caatagattc tgaacttttt gggcatgtaa aaggtgcgtt tacttcggct
     1801 catagcgata gaaaaggtta tttcgaagaa gctaacaaag gaacaatatt tttagatgaa
     1861 gttgccgaat tgcctttagc tactcaagtg cgtttgctta gagttttaga aattggcgaa
     1921 tttataaaag taggttcttc aaaacctgaa aaaactgatg ttagagttgt agctgcaaca
     1981 aatgttgatt tacaacaaag agttcgtgaa ggtaaatttc gcgaagactt gtactataga
     2041 ctgaatactg ttccgattgt tgtacctcct ttgcgacaca gacaagacga cattcatttg
     2101 ttatttagaa aatttgcaag cgatttttcc gaaaaatata atatgccggc aattcgccta
     2161 agcgaagatg cagttcatgt tttaaattca tatagatggc ccggaaatat tcgtcaatta
     2221 aaaaacatca cagagcaaat ttctataatt gaagaagaaa gaattatttc tgcagaattg
     2281 ctaaaatctt atttagaagg tgcagaaact tcgaatttac ctgttttatc aaaaaaaggt
     2341 aattatgaaa attttgcttc agagagagaa atactttatc aggttttgtt tgatatgaaa
     2401 agagatatga atgaactcaa agttttggta aatgacatta tccaacaaaa tcctaatgtt
     2461 aaaattacta attatccaaa taacactttc aataattttg ataatgaagt acaaataaat
     2521 cctataaatc acgtctattc aaatgaacaa ccagttattc atcatcacga gccacaccaa
     2581 gaacacggcg aggttgttga agaagtttta tctttagctg aacaagaaaa agaattaatt
     2641 attaaagctt tggagaaaca taataataaa cgcaaactag cagcagaaga attaggaatt
     2701 tcggaaagaa ctttatatag aaaaattaaa gaatatcaaa taaataagta aatgaaaaat
     2761 ataagaaaca tagtttattt tttaattttt attagctcta ttgctttatg ctcttgtact
     2821 ggaggatatt cgtttacagg agctgatatt tctgctgata taaaaactat tcaagttaat
     2881 ttttttccaa atcaagctag tatggttcag ccaaatcttt ctacagtttt taccgaaact
     2941 ttgaaagata aatttatttc tcaaacaaat cttgaaatgg ttaactacga cggcgatatt
     3001 gcctttgaag gtgcaattat aaattataat gttactgctc aatcttttca aggaaacgaa
     3061 accgctgcct taaatagatt aacaattact gttaaagtta aatttacaaa tacaaaagaa
     3121 acagcaaaaa gctttgaagc aaattttaca agatacgctg attttgaaag ttctataaat
     3181 ttagcaagca ttgaaggcga gttaatccaa agcatttcgg aagaattagt aaccgatatt
     3241 ttcaatagag ctgttgcaaa ttggtaaaat atgaataagg aacaattttt aaaatattgt
     3301 agcgattttt ctttactaga ctcaaaaagt ttagaagata tttctgctct tattgaagag
     3361 ttcccctatt tccagactgc cagaatttta tatgctaaaa atctaaattc gctcaaagac
     3421 gtaagatttg aaaataaatt gagaaatacc gtagtttatg ttccagacag aaaacatttg
     3481 gttagactaa tcaatttgaa aaatcttcct gaaaatgaaa taacattcat agatgagcca
     3541 aaacttgttg atcaagacat acaaattgaa gaaacaaacc aagaagaacc tgctgaaatt
     3601 caacctgtca accaagaaat cgaagttaaa caagaagaaa taattgaaac ccctcaagtt
     3661 agccaagaag ttgaaactaa agaagtaaaa caagaagaaa tcgctgataa acagcaaaca
     3721 gtccaagcag ttgaaatcga agaaaccaaa caagaagaag ttgttgaaat tcatgacatt
     3781 agctcagaaa tcaaagaaga agaagttact gaaaatattt tacctgaatt tactaacgat
     3841 gttcaaactg ttgacgaagc acctaatgat gaaaaacttg tagaaaaaga accaacacaa
     3901 caagaagatt attcagaaga tttgtctgaa acagataggc ttaaatcaat tattgaagct
     3961 cgattgagag aactaggcat taacgaaaaa gttagaatag gaaatgaaga aactgaggaa
     4021 ataaaacaag agagcactgt aaatcagcaa gttaatcaaa aaaaccaagt tgaagaaatt
     4081 gaagaagatg ttgtcataaa ccagcaaatt agtgaagaaa tcaaagaaga agcatcggaa
     4141 aatattttta tagatttttc tgatgatgca aaaaaaattg acgagtctat aaataaaaat
     4201 aatggttttg agcttttaga ttttgatttc gagatagaaa aagatgcaat tttagagcat
     4261 caaaataaaa aaacagatgc aaaagactat gatattttag aaaatttttc tcaaaaaacg
     4321 acaaataata agcctgaaaa aataaattta atagataagt ttttagctga aaatccgaga
     4381 ataattccag acaaagaata cgtttctgac aattctcttg caagcaaatc gctacacatt
     4441 gacgatagtg atttatttag tgaaacttta gctaaaatat atattaaaca agagcatttt
     4501 gagaaagcaa ttttaactta tgaaaaatta tgtttgaaat atcccgaaaa aagtatttac
     4561 tttgtcgggc aaattgaaaa aataaaagaa ttgataaaaa ataaaacgaa ttagttatgt
     4621 atatcttttt ttctgtatta attgttattt gtgctgtact actaatttta atcgtattag
     4681 tacaaaatag taaaggtggt ggtttagctt caaactttgg acaaggtaac caagttatgg
     4741 gtgttaaaaa aactgctgac tttcttgaga aagccacttg gggtcttgct ataggattaa
     4801 ttgtattttc tttattagca agtttttcta tacctaataa ccaaaaaatc aaaaagcaag
     4861 attttgaaca aactcaaact gcaccagcag aaagcagtga accagcatct ggcgctggag
     4921 agcctcttcc tgttgaataa taaaaatatt tagtagttta caattttgta gtttttgtat
     4981 ttttacttat attttttcaa cctaattata aagtcgctta aaatattcat atagtttacg
     5041 aatgcttcgt atggcgactc ataagggttg aaatatttat gtacgtctcc gtcagagaac
     5101 cattttgtgc acatataata aaagtggtcg ctggtttgta agtacaacca atctttccag
     5161 atttctggat cgtttatttc ttttacttta tcataaagcg aatataagtt attgaaagcc
     5221 tcgtcttgga gttcgtttcc taaccaagca gtaaggtctc tttcctcgtc tgcccaagaa
     5281 attggatatg gcacatgaat ttttgctaca ggttcagcat tttcaacaat ttcagatgga
     5341 gtagaaaaac tatagtttga atatctaaaa acagcatcag gaagagcttt taaaaactcg
     5401 aaaattcctg tttcttccca ttgatgttcg ccaaaagttt cgtagtccat aaacagattt
     5461 accatttctt cttttgggtc aattgcattt agccatgaca caaatttttc tgttgtcaat
     5521 ggaaattcct cccaagcatg atttgaaaaa cggaaagcta tatcatcaga aagcttaaaa
     5581 tttttaagca ataattttaa ctttggattt attgcgttgc agtacagata gtttggactt
     5641 ttccaaccta aaatatgctt tgctccttct gtaatcattc ccttaaaacc catatttgag
     5701 atatactctc ctatttggtc ggaatatatt aattctgtat ttctaaaaat tttaggttca
     5761 actccaaaat gctcttttat tttttggcga tgcatattaa tttgacgttc aaactcctcg
     5821 tagcttttta aagcagaaag agagtgagca taagtttcgc taagaatttc gacattacct
     5881 gtagctacaa gtcttttgaa actctccaaa acatcaggtg cgtacatctc gaattggtct
     5941 attgccactc cagaaataga atatgcaatt ttaaatctat cgccatattc ttgaattaaa
     6001 tccaagaaca cttgatttgt tggcagataa cacttttctg caacttttcg cattatatta
     6061 cgattttcaa aatcattgta ataataatgg tcgttgccaa tatcaaaaaa tttatatctt
     6121 tttaacctaa atggctgatg cacctgaaaa taaaaacaaa ttgtacgcat tatattattt
     6181 ttttaattta cacattatta attattcaat tatcaattat tcaattattc aattattcat
     6241 tcttaattct taattaaatc attataaacc ttatttactt taaacgctga attttcccat
     6301 cttaaatttt cgacttctat ttttccatat tttctaaaca ttcttgacag ggctggataa
     6361 tgcaacattc cataaatagc atctgctagg tcatctacat cccaaaaatc gacttttaaa
     6421 gcatgtttca acacttctgc cacacccgat tgttttgaaa taattacagg cacgtcagac
     6481 atcattgctt ctaatggtga aattccaaaa ggttcgctaa cagacggcat tacgtaaaca
     6541 tcgctaatcg aaaacatatc ataaacttct tgtcctcgta aaaatcctgc gaaatggaat
     6601 tttgttgcta tatttaattt tgccactcgt tttatcattc tatttagcat atctccactt
     6661 cctgccatta caaaacgcac atttgggtct ttatcgagaa cttttttagc tgcttcaata
     6721 aaatattcag gacctttttg gaaagttaca cgtcctaaaa aagtaacaat tttctcatca
     6781 aaaccttttg ttaccctaaa ctcatctggc aaagttattg gctctactga attatgaacc
     6841 gttacaacct tatcgggatg gattccatat ctttcgataa tagttcttcg tgtgagatta
     6901 cttacagcaa taactctatc tgctgcttcc attccttcac gctcaatatc ataaactctt
     6961 tgatttacat tttccccact tctgtcgaat tctgttgcgt gcatatgcac aactaaaggt
     7021 ttacccgaaa ctttttttgc agcaattcct gcatgatatg taagccaatc gtgagcatga
     7081 ataacatcaa actggtgtgt gcttgcaata taagcagcaa tcaaagcata acgagcaact
     7141 tcttcataaa gacttgcacc atagcttcct gaaaacttaa agttttttga cagtacagat
     7201 tctttaaatt ctgaatgaga aagtgatttt cctgcgaata tttttttaaa ttcttcgggg
     7261 tccatatatg gaattaagtt tgcccccact tcaatatatc taaaattttt ccagaatttt
     7321 gtttctttag ttgttctttc tgttatatca acttgtccag catcaacaag atttatataa
     7381 gttttatcct catctccata agattttggc actacaaaaa ttacttctgt accaaagtgt
     7441 gataatcctc ttgttaagcc ataacatgcg gttcctaatc ctcctgtgat atgtggtgga
     7501 aattcccaac caaacattag tactctcatg attttatatt gtttttgtaa ttatcaatca
     7561 tgttagcaat tctcagcaat tctgcaacac tccaagcctg tgcaattgct ccacgtgcat
     7621 tatgtggtgg gtctccgtca taaatttccg aaatatttcc tattccagca attgtcatat
     7681 cttcttcaaa gttcaaataa attttttcta ctaaacctaa accactttct ccatgaattt
     7741 ttagccagcc ttcacaaaaa cttcccaaca accagggcca agcagttcct tgatgataag
     7801 cattgtctct ttgttcttga ttgcctttat aaaccccttt ataatcaggc gaactagggc
     7861 ttaaagttct taaacctttt ggtgttaaaa gttctttctt aactttttct aaaactcttt
     7921 ctcttttttc ttccgacaaa ggtgaatatg gtagcgagca agcaaaaatt tgatttggac
     7981 gaattgccca atctttatat tcataagtgc aataatcagc gagatagtgt ttattgtcgt
     8041 cccagaaaaa ttgtaaaaat gatgattttg ttatttctgg cacatgtttc caagcctcaa
     8101 taaaatcttt gtctttatta tgttctgcga gttctaagct aaatttaaca gcattatacc
     8161 ataaggcatt aatttcaact gcaaatccgt gtcgtggtgt tacaggtttt ccagcaacaa
     8221 ctgcgtccat ccaagtaagt gcttttcctg cttctcctgc ccaaatcaag ccgttatcgt
     8281 gcatttttat gttaaactgt gtgccacttc ggaaattttc taaaatattt ttaatttttt
     8341 cgccaaaatc tttccagatt ttaactttat ccttggtaaa ctttaaatac tcttgtaaag
     8401 cccagaaaaa ccataatggt gcatctacag aatttagtgc agcattattg ccagctccaa
     8461 tatttggaaa aagtccatct ttaatttcag aagtcatagt atctaaaact tctttacatg
     8521 tttttatatc attttgatat aatgttaatc ctggtagtga aataaaagta tctcgtcccc
     8581 atctgccaaa ccatggatag cctgcaacga tttcactttt tccttttctt ttgtaaataa
     8641 attgtttagc tgcactttcc aaacattctc tataattagt acgtggcatt cttttagcaa
     8701 tttcggcatt atattttctt gctaaatttt taggatttgc ctcatctaat cctgctgtaa
     8761 aaattatagt ttcacctgtt tttatatctg cctcaaaata tcctggcaca taaaggtctt
     8821 cttgataatc atatcctcgt ctttgctctt cctcatactc aacattataa taccagtcgg
     8881 gtgcatgtat atactcagct tctttatttg tttgtaagta aagaaattta taagcatcat
     8941 acattcttag cttgatacca ttgttcacag gtgtaaattt tgtatttaca aacaaattgg
     9001 ctttactaag tttatgcaca tttctaaaag cgagaaaagg tttaaatcta attttggttt
     9061 tactaacagc ttctaacaaa gtatatttaa taattattct atcatcttcg gtaaggattc
     9121 tttccatttg taaaaccacc cctcctactc tccatacaag ttttggaaca acatctatat
     9181 aaaaatctct tacatattta tgtccttttg gattgtaatt atttcccggg tattttctta
     9241 caccaaaatt aaaaatagca tcattttgaa ttactgaaat atctatacca gaaagtaaaa
     9301 catgattatc gttatcgata tcttccatag gacaaaccaa aagcccatga tattttcgag
     9361 tattacagcc aactattgta gtactcgcgt aagacccagc tctatttgtt cttagtaatt
     9421 ctctctttag cgagtattca agatttatta attgttcctt atcaaattct atataattca
     9481 tgcctaaaat tttacaaatc taacacttaa ttattaaacg taacttttct attctatcgt
     9541 ccaaagataa acaaaaaaaa tgacataaca atttttattt tcaattattt ttaaaatatt
     9601 tttagttaat taaatttcaa ctttattttt tcatcatcgc gtgctttcgt tctcattaac
     9661 aagcttttaa gttagaatat aattttaatg tatttaacaa ttgtttttta ataattttat
     9721 tttgtaaaaa tcatagctta atgtaacttt gaacaataaa ttaacaagcg ataatgataa
     9781 gaaataattt tttagaatcg tcaaaacttt tgaatgagtt tatttcagat aataaaaatt
     9841 ttgaaaccat tgaatcagct gcgaaattaa tgattaaaag cattaataat ggtggaaaaa
     9901 taatttcttg cggtaatggc ggttccatga gtgatgccat gcattttgcc gaagaattat
     9961 ccggcagatt taaagaggaa agaaaagcac taccagctat tgccattgct gacccttctc
    10021 atataacttg tgttgcaaat gattatggtt ttgattttat tttttcgaga tttattgaag
    10081 ccttgggaaa taaaaatgac gtattacttg caatcagcac aagtggaaat tcaaaaaatg
    10141 taattaatgc tgtaaattct gctataaata ttggaatgcc tgttgttgct cttactggca
    10201 aagacggtgg caagctaaaa aatctttgcg acattgaaat tagagttcca cattttggat
    10261 attctgaccg aattcaagaa atacacataa aagttattca ttctattatt gattatatcg
    10321 agacaaatat tgataaataa atttaaaaca tgactattca ctttattgct ataggcggtg
    10381 ctgttatgca caatttagca atagctttac ataaaaaagg ctatcaagtt tctggctctg
    10441 atgatgaaat atttgagcct tctttttctc gtttgaaaaa acacggactt cttcccaaga
    10501 aatttggttg gtttccagaa aaaataaatg ctgatttgga tataattatt ttgggaatgc
    10561 atgctcgtaa agataatcct gaacttttac gtgctcaaga acttaatctt aaaatttatt
    10621 ctttcccaga atttttatac gaacaaacca aagacaaaaa acgtgtagtt attggcggaa
    10681 gtcatggaaa aaccactata acatctatga ttatgcacgt tttgcgagat gccaaactaa
    10741 attttgatta tatggtaggt gcaaatattc gtgggtttga tactatggta aatcttgaag
    10801 aaaaaaacaa agttgcagtt tttgaaggag atgaatatct ttcttcgcca ttagatttaa
    10861 ctccaaaatt tcattggtac aaacctgata ttgccgtact tacaggaatt gcttgggatc
    10921 atataaatgt ttttcctagt tttgaaaatt atgtggagca atttgatatt tttattaaac
    10981 atattagcaa aaacggaaaa tttttttggt ttgcagatga tgaacatctt caaaaattag
    11041 taaaaaacaa caatgttgat aataaagcat atttggcttt tgaacataaa gtttctgaag
    11101 atggaaaaac tttagtaaaa tttgatggta aaacttatac tatgagtgtt tttggaaatc
    11161 ataatatgca aaatataaat gcagcacgat tagtttgttt agaattaggg attaaagaaa
    11221 aattcttttg ggaatctatt caaacttttg agggtgcatc taagcgtttg caactgataa
    11281 aagaaactga aaactcatca ttttatttag attttgctca tgctccttct aaactaaaag
    11341 caacaattaa cgcaataaaa aataaaaacc ccgacagaaa attaatagct tgtattgagc
    11401 tacatacttt tagtagttta caacataatt ttattcctca atacaaaaac acaatggacg
    11461 aagccgacat tgcaattgtt tattatagcc atgaggtgct agaacacaag aggttaccag
    11521 ccttgcccga agattatgtt aaagaaagtt ttggtggaaa agttcaaatt tttactgatg
    11581 ctgaactact gaaagattac ttagcaaaac aaaattatga taacacaaat gtgctaatga
    11641 tgagcagtgg taattttaac ggtatagatt ttgttgattt tgcaaataaa ttgatttaaa
    11701 atgaaaaaat atttttttag cttaataata gtttcgattt tagttagttc ttgttcgttt
    11761 aataaactgc atattggtca gcccgaaagc ttggaagttg aagaactaag catgcaaggt
    11821 gttaggctga aagtttacgt tccaatagaa aatccaaata atttatcttt taatgtaaaa
    11881 aacttaaatt tagacttaat tttaaacgac agaaatgtag gtaagattaa aaaactgaaa
    11941 aaaacaaaaa ttcttaaaaa ttcaaaacaa acatatccat tttcatttga ggtaacgcca
    12001 aaagatgcat tgacaaatat aatatattta gtaagcgaac ttcaaagacg aaatccagag
    12061 ttaaaaatag atggcagtgt aactgtttct aaaatgttga tttccaaaaa gattaaaatt
    12121 gaccataaag aaagttttga taaatattat aaaaagtaat atctttacac aaaaataatt
    12181 aattatgagt aggtttttat cgattatatt attgttttta tcaattggga ttgttgcaaa
    12241 atctcaaaac ataacaccta tgagcatttc ttattttaat tcttctgcct ataatggtaa
    12301 agttaaaatt aaacaagatg ctaaattgaa aaattttgtt gaaactagtg taggtttaaa
    12361 taaaaagcgt aacggttttt atggttatag agtaaaaatt tatgcagaaa accaccctaa
    12421 tgctcgttct caagcaaata ctgttagaac tggttttaga caagatggcg aaaaagcata
    12481 tttgaatttt atagaaccaa attttgaagt tttagttggt gatttcacaa accgttttga
    12541 tgctgtaaaa cttctaaaaa agataaacac aaaatatcct gacgcttatg ttgtaaaaac
    12601 aatagtttct tacccaaatc attagttttt acccgaagaa tttttttact tcttgagcaa
    12661 atcttttatc tacttctttt ccgtctctag ttcttgctaa gccacctctt ccagtttctt
    12721 tatagtctat gtgcagtctt tctcccactt ctctcattgt taacataact tcgtctggtg
    12781 agataaaaga ttgaactcca cttaaagcca ttaaagctgc tgaaagtgct aaagatgaat
    12841 aaattccatt tcttttcaca caaggcactt ctactaaacc tgcaactggg tcgcaaatta
    12901 atcctaagca attttttata gcaattgtaa aagcctgaat aagttgtgca tggttaccat
    12961 tttccaaata tgctattgcc gaagctgcca ttgctgcagc tgctcccact tcggcttggc
    13021 aaccataatc ggctcctgct gtagaaacgt ctgtaaataa tatcattcca aaaaagcctg
    13081 ctaccatcaa agactctaga attttctttt ccgaattagg attgtcttcc atccaagctg
    13141 ttaaaacacc tggtaaaata ccacaacttc ctgcagttgg acaagctatg ataacaccac
    13201 tttttgcatt tatctcattt actgctgtag cataagctgc tgccctgcca taaacattat
    13261 taaacaattt tgattgtggc aaataagaaa ctattttttt agaatcacca ccacttagtt
    13321 ttagcaaaga ttgttctgat gttgaaattc ctgaataaac agaatctttc ataatttccc
    13381 aatattcttg cattttggaa taaacatctt ctcttttcat tccttgcaag gcaaattcag
    13441 tttcgcaaat aacatcaata atattttttc ctgttctttc taagtattcc tctaattctt
    13501 caaaagtatt aaactcaagt cttggttttt cgctttcggt ttgttcattg taaattttac
    13561 gaattattgg ataattttcg tcttttgaac ttacaatatc attacctatt aatcttaaat
    13621 ctgattttcc atcaatagga gtttggagtt cgtggtcgtt aaaaaatcta atttccacat
    13681 ttcctgcacc aattgaaaag ccaacgattg agtaagtatc tttatctgtt gagaaaataa
    13741 cttctgctat attattattt cgttgagata agtatttcaa atcttcagat ggacttgctt
    13801 taacaaagcc tttaaactta gcggtttgtt cgccaaattt tattccattt tcttttaaaa
    13861 atattgggtc gccatgttca atcatttctg ggtggtcggt agcaaaacca tacaaaccac
    13921 ctgctaaagc atttggcgtt ctatgtccaa ctcctgtatc gcgaaacgaa gaataaaatt
    13981 taagttcaac atctttaatc acttcgcctc tttcaataat tgctaaaatt atatttctac
    14041 ttatttgtcc aattctattt gctccggcag tatgcgaact tgatggtcca accattgcag
    14101 gtccaatcat atcgaaaata cttaaagcct gcatccaagt ttgaatatac ctttcaaaat
    14161 cttcttttaa ggaatgaaaa tcttcttctg aaatttgttc atcaagatga atttctactt
    14221 tataatattt gttattccta tttaaaggtt ttgcaagttg aatgatagaa agaaagctat
    14281 atttaatggc ttgtttttct atgctttcaa aaagatttga caataaagta ttaaaagcaa
    14341 tattcataca ttgaactaca acttttcttg catcatcttc taaatgaact tcaatatctt
    14401 ttattcgttt tcctttttca tcataaaaac tttgtctaat tttttcttct gaaggtagtt
    14461 caaattcttc aagtcttacc gaagattcaa gacttctata tgttgatgat aaaataaaat
    14521 cttctttctg attagggaaa actctgtaaa tagcaatatt ttcaacaact tcagcagctt
    14581 cttttaataa agtctctttt aatgtatcat aacaaaaact atgagaataa aactcttcga
    14641 tatcgccttt catatggaac aatttgtcct tgccttctgg agctattgtt cgttttatta
    14701 gtttgtaatg agtaacaaaa tcttcttcgt ccataatttc aaagaaaatt ggtggcaaac
    14761 aagatttcaa aaaagttgta tatttcaaca ttttatgttt tggtatttgt gtttccatat
    14821 tttcaatttt ttatatttat atttttaaca tccatttatc aagtgttcta ataatatttt
    14881 tattcctatt cctattaaaa aaagtcctcc tattatttct actggcaatc tcttgaactt
    14941 ttttcgtaaa aaattgccac ttattagtcc taaatatgat aagataaaag tagtaaaaaa
    15001 aactataagt gctgtcagcc aaatattaat tcctacaaaa ggcaaactta cccctaccac
    15061 aactgcatca atacttgtag ccacaccaag accagcaaca acaaagaagt tattcatatt
    15121 tatttcggct ttgtcttctt tttttcttat tccatcaatc aacatttttc cgccaataat
    15181 tgtcaacaat gcaaaagcaa tccagtggtc atattgttga aacatttttg ttaaacttga
    15241 acctgcaagc caacctacga caatacaaat tgcttgaaca aatgctaaaa taagtgcaaa
    15301 tcttaaattg gtgatttttg ttttattttc gcatgaacaa gctgtcgcta ttgaaatcgc
    15361 aaggctatcc attgaaagcc ctatagatat ta
//
LOCUS       DAOPNX010000063        15373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_4580_length_15373_cov_5.849915, whole genome shotgun sequence.
ACCESSION   DAOPNX010000063 DAOPNX010000000
VERSION     DAOPNX010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15373
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4580_length_15373_cov_5.849915"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1423
                     /locus_tag="PKN53_07740"
     CDS             <1..1423
                     /locus_tag="PKN53_07740"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HOD88876.1"
                     /translation="GFGKNDVIFNPEYYVSDGESQSVYQHFAGDLNYMKKFNNNGHEL
                     QIYANYSATQDKSLDYYSTYDTDSIYNNIGKPENEYRTKEDGSGNMFLAKADYVYPIT
                     EKAKLEAGYQLRTSKTTNDYRYQTMQSEWIDDPTQHNPYVYTMNVQSGYVIFSNYFKT
                     LGYQLGLRTEYTDRVFLQTLTNQEWTNADQNKFDFFPSVHLSYQFPADFQILASYSRR
                     LGRPRPWFLNPFIEVVDPNTVRVGNPYLKNEYINSFDLSFQKKFGAHYVAIEAYYRET
                     VDKINRVNRIDSNNPDIFISTFDNIGKDISTGAELMTNLNVTKWYNLNISGTGYYYEI
                     IAGSNNYNSNNSIQWNARINNTIKIKKTGTGIQLGAFLNGPRLSAQGKSSASWAAGGG
                     VRQDFLDRQLSVSLNLRNIFRTMKMERISETDSFSQHFLRQPRGPIVTLSVTYKINDF
                     KSRREKGFTNEEPGRGGGDDEGM"
     gene            1526..1798
                     /locus_tag="PKN53_07745"
     CDS             1526..1798
                     /locus_tag="PKN53_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOD88877.1"
                     /translation="MKKGIHPEKYRLVAFKDMSNDNVFITKSAANTKETIEIDGVEYP
                     LYKLEISNTSHPFYTGKMKLVDTAGRVDKFRNRYKDHFERKNQKKA"
     gene            2076..3239
                     /locus_tag="PKN53_07750"
     CDS             2076..3239
                     /locus_tag="PKN53_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HOD88878.1"
                     /translation="MNFILSDFNKHQKFLPLTFTRALADYRIGILTLKEKWEFYLNQK
                     VSVLTQEYLQAKYSLKIENENIVINAQIIPNPELVGEILKLKDACLVKDENIIAYSCS
                     DNLEDIPKTKIEFKGEVLIINSNTDVFTFNGELIKQDFDIITKGRKSQKLSSTNTVFG
                     KYPVFVEEGCFVECASINTNDGPVYIGKNAKIMEGSHIRGAFAICENSELKMGAKIYG
                     PTTIGPSCKVGGEINNSVFFAHSNKAHDGFLGNSVIGEWCNLGADSNNSNLKNNYAIV
                     KLWDYEKERFVSTGLQFCGLIMGDHSKCGINTMFNTGTVVGVSANIFGAGFPRNFIPS
                     FSWGGHAGLSVYKTETAFEVMTKVMERRNVQLTEIDKQILAHVFDITAKYRNF"
     gene            3370..5778
                     /gene="lon"
                     /locus_tag="PKN53_07755"
     CDS             3370..5778
                     /gene="lon"
                     /locus_tag="PKN53_07755"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOD88879.1"
                     /translation="MGAGKEEAEFIPIVTEFNEEEDDTNHPETLPILPLRNTVLFPGV
                     VIPLTIGRSKSFKLIKDIHKTTKFFGAVAQKELEIEDPKPDDIYKVGTLAEILKVIEM
                     PDDTISVIIQGKKRFNINQYLTDEPYHMATVSYIAEDKTIESKRSFQAIVSSLKDMSL
                     KIIKLSASIPQEASFAIKNIDNPSFLINFICSNSDIETAEKQKLLTENSLYKRAEKLL
                     GILVNQVQMLELKNDIQIKVKKEIDKQQKEYLLHQQIKTIQQELGGGPAEQEIKELEE
                     KAKSKVWKKEVKEHFERELKKLQTLNTASAEFSVQLNYLHTLLDLPWNEYTVDNFDLK
                     HAQKVLDEDHFGLEKVKERIIEHLAVLKLKGDLKAPILCLYGPPGVGKTSLGKSVARA
                     LGKKYIRMSLGGVHDEAEIRGHRKTYIGAMPGRILQNIKKAKSSNPVFVLDEIDKVGS
                     DFKGDPSSALLEVLDPEQNTEFHDNFVEIEYDLSKVMFIATANNVQTISPALRDRMEM
                     IEISGYIIEEKIQIAKRHLIPKQLENHGLKDLKLKIDDKALVKIIEGYTRESGVRGLD
                     KNIAKIIRKIALKIGLNEEVPNKITEADIVEYLGQEKFLKEEYQDSEIAGVVTGLAWT
                     AVGGDILYIETSLSKGKGELTITGNLGDVMKESATIALQYIKANAEVYDIDPKLFETY
                     NIHLHVPEGAIPKDGPSAGITMATAIASAFTQRKVANKIAMTGEITLRGKVLPVGGIK
                     EKILAAKRAGIKEIILSKENEKDITEIKDIYIKGLKFHYVSNISEVLDIALLKQKVRN
                     AKKL"
     gene            5799..7094
                     /locus_tag="PKN53_07760"
     CDS             5799..7094
                     /locus_tag="PKN53_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOD88880.1"
                     /translation="MQRKNIDLMAPVGSYESLMAAIQAGANSVYFGIGNLNMRSRSSI
                     NFTFEDLRKIVNICNEAGVNTYLTVNTVIYDGELDTMRQIIDAAAEVKVSAVIVSDVA
                     VMKYAREKGVEVHASTQLNIANIEAVEFYSQFCDVMVTARELSLDQVADITYQIKQRN
                     IKGPNGKLVEIEVFCHGALCMAISGKCYMSLHEFHHSANRGACLQNCRRTYILKDKES
                     DTEIEVDNEYLMSPKDLCTISFIDKIIDAGVSVLKIEGRARSAEYVKTVVECYDQAIK
                     SVLDNTYNQEKIDEWTKRLSTVFNRGFWNGYYLGQRLGEWAKQHGSKATKTKVFLGKI
                     LNYYSNIGVAEIKLETDEELLVSDEIVILGPTTGVVQQFVEEIHFEQKSVKKVKQGDL
                     FSIKTDELVRRGDKLYKLEDTEELKKRNRERKYQINNDE"
     gene            7084..7530
                     /locus_tag="PKN53_07765"
     CDS             7084..7530
                     /locus_tag="PKN53_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOD88881.1"
                     /translation="MMSNNNIPERVVKFINQHHVATIATCVDSESWCFNCFYAFVESE
                     GALIFTSSTDTKHIKMVEKNPIVSGSIVLETKIVGKIRGLQFVGEMQKVENTENSKYR
                     LKYLAKFPFAVIVNSDLWILKLTELKMTDNRLGFGTKLEWKKSVES"
     gene            complement(7581..13148)
                     /locus_tag="PKN53_07770"
     CDS             complement(7581..13148)
                     /locus_tag="PKN53_07770"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOD88882.1"
                     /translation="MNKTRIFYWISGAILLGVVIFAIVSMFGSDKKVKLSDPSLYKDY
                     IAAYSNFVISKNDVITVQLTDEFAYKVKSQKTKILTIEPKVKGELTWKENNIIEFKPE
                     KPLQSGQAYCVKLNLRKLDSSVPKELKEFVFKVQVKHQFIDLNIDKVITTDRDNFTKQ
                     NVGGTIKLTDSENLENIKKCISAELNGKKLDIEIESSNNNEFEFLIKDITRTNNSQTL
                     KITCNGKEINSNSKFSREIEIPELGVFKVLDIQVNQYPEQYVNIIFSDPVKEDQLLEG
                     IVDIEDVYNLKYIIVDNNVKIIPSDRLDNDYLLNISGVKNIHDKYLDEPVSKELNFKM
                     RKPELAFDNDGVILPTSSQGQVISFQAVNIKTVHIRVIKIFQNNILQFLQDNSLDESY
                     SLNRVGRVVKTQTLSLEQADIDDIGEWNTYFLNLSDLIEPDPGAIYRIEIGFIKENAI
                     YPCDNSTNKDNKEDDNLNKIDADSEWKWFTDYYYSDYSSDYYYDDEYYYYDDYYYDDY
                     ENSYDDPCKSEYYGYRKALKFNILATNIGIVSKIGSDNKVFAFVTNILTAEPITGAEV
                     LAYDYQQQVIAKATTNTNGQAELIISNNLKPYFIVASFNNQKSYLKLEYYNSLSTSEF
                     DVWGDSSQEGTKAFIYGERGVWRPGDSIYLGFILHETYNPIPEGHPIVLEVKNPKGQL
                     VYTGKQNKNKKGFHVFKFKTEPNDPTGYYNAKFIIGGKVFTKSLMVETIRPNRLSVEL
                     GLDKNYLKGNSTVNAMVSAKWLHGAPAVDLQTTIVMSLERKYTPFQKYEDYCFENQTA
                     NFNFNSQTIASGKTNADGEFKQLLKFNDIKNAPGVLNATLTTKVFEKGGEFSIDESTA
                     VYYPYDAFVGVKVKESDEYSYSLPTGKNLNLDIVLLDLNQKLIAENRDLEISMYKLDY
                     SWWYDFQRDGVDYISANYNNAIFRENQKCKNGKSNFGFIINESGNYVIAVKDLKGKHI
                     AALKIYASSYASESDKGSVEMLKFSASKEKYNVGETVEIEFPAHTGHALVSIENANTV
                     LNSFWVKSDGSKLKVEFEATQEMAPNVYVNIIYLQEHLRTIKNDRPLRMYGIIPIFVE
                     NPETHLNPELVMNNELLAESKVKIQVKEKDGKPMSYTLAMVDEGLLNLTKFKTPNPGA
                     YFYSKEALGVRTWDIFNYVIGAFKIDAGKMLSIGGGDDEVAPEDLAQANRFRPMVKYI
                     GPFELKKGETKTHEIQLPQYIGSVRTMVVAAEGNAFGSAEKTTPVKKPLMILGTAPRR
                     LGVNESFKLPVTVFAMKPDVKNVKLTIKTNEMLEVVGSSSATVNFIKEGEKIHNFSLK
                     SNTKTGIAKIEIIATSGSHVSKYNVELDVKHVNTKVAEIIDEMSGQNEVNITFDADGI
                     TGSNEAYLEIYNIPPMNLEYRLNFLKTYPHGCLEQIVSAVFPQLYLDAFMELTAEQKA
                     EIQTNINACLNKLNKYQTASGGFAYWAGLNEVSEWATNYAGHFMLEAEKSGYTVNSSV
                     KSNWLKYQKRKASKWVDDGNTSQLTQAYRLYTLALAKQADRSAMNRLKESKIQSATAW
                     RLASAYALIGNTSIAKSMIAKLNTNVNSYYELSGTFGSGIRDKAMILETLIELGEKEK
                     AFMVLKDIAEFMGSNQYASTQTSAYALLASAKYVKKWAVSEIIECNYTFNGTKYSVKT
                     NKPVYKTKLKIFENEKNNFVLNPTENNSYFVRIIKKGIPEIEHETAGSSNINMSIKYT
                     SLTGNPIDITNIPQGTDFVATVTISNVSNLASLDNVALSQVFPSGWEIINSRMFTTQL
                     GDDSYYTYQDIRDDRVLTYFNIYNKYTYTYRVMLTAAYEGKYYLPATVCKTMYDDSNF
                     ARTKGQWITVSGF"
     gene            complement(13260..>15373)
                     /locus_tag="PKN53_07775"
     CDS             complement(13260..>15373)
                     /locus_tag="PKN53_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOD88883.1"
                     /translation="KMTMELDDFSDVSSSRENDDDDDEEDSYRPSRSSSSPRKSSTSS
                     KSSTPMLDSFGIDLTQAVIDGKVDTIVGRDTEIERIAQILSRRKKNNPVLIGEPGVGK
                     SAIVEGLATKIVQKRVPHLLLDKRVVILDMATIVAGTKYRGQFEERMKAILDELIAAP
                     DVILFIDEIHTIVGAGGLPGSLDAANILKPALARGEIQCIGATTLDEYRKSIEKDGAL
                     ERRFQKIIVEPPTAEETLDILKKIKDKYEDHHKVSYTDKALEACVKLTTRYISDRALP
                     DKAIDAMDEAGSRKHISSASIPNKIIKLEEDLQKIKDEKIKAVTQQQFEKAAAMRDQE
                     RGIERHIELAKENWLKELNEVREIVNDDDIAEVIAMMTGVPVKRIAQSEGNRLLNMDE
                     DIKGSVIGQDEAVKKIVQAIQRNRAGLKDPNKPIGTFIFLGPTGVGKTQLAKVLARYL
                     FDSEDALIRIDMSEYMEKFSVSRLVGAPPGYVGYEEGGQLTEKVRRKPYSIVLLDEIE
                     KAHPDVFHILLQVLDEGRLTDSLGRRIDFRNTILIMTSNIGTRQLNEYGRGVGFESSA
                     KKASYDASAKTIIENALKKTFAPEFLNRIDDVIMFNALTKEDIFKIIDIELKGLYKRV
                     KELGYNLKLTPAAKNYVAEKGFDPKFGARPLKRALQKYVEDAMAETIIKAEIQKGDSI
                     TVGFSEKTGITIKITKEEAET"
BASE COUNT         5240 a         2363 c         2425 g         5345 t
ORIGIN      
        1 tggctttggg aaaaatgatg taatttttaa tcctgaatat tatgtgtctg atggcgaatc
       61 tcaatctgta tatcaacatt ttgcaggaga tttgaattat atgaaaaagt ttaataataa
      121 tggacatgaa ttacagattt atgcaaatta ttctgctaca caagataaaa gccttgatta
      181 ttacagtact tacgatacag atagcattta taataatata ggtaagccag aaaacgaata
      241 tagaactaaa gaagatggat ctggaaatat gtttttagca aaagctgatt atgtttatcc
      301 aattacagaa aaagctaaac ttgaggctgg ttatcaactt agaacatcaa aaacaactaa
      361 tgattatcgt tatcaaacca tgcagtcaga atggatagat gacccaacac aacacaatcc
      421 ttatgtttat actatgaatg tccaatctgg ctatgtaata ttttctaatt atttcaaaac
      481 tcttggctat caattaggct tacgaacaga atatacagat agagtatttt tacaaacact
      541 aactaatcaa gaatggacaa atgccgacca aaacaagttt gattttttcc cttctgtaca
      601 cttatcttat cagtttccag cagattttca aattttagca agttatagcc ggagattagg
      661 aagacctaga ccatggtttt taaatccttt tatcgaagtt gttgacccaa atacagttcg
      721 tgtgggtaat ccatatttaa aaaatgaata tattaattct tttgatttga gttttcaaaa
      781 aaaatttgga gctcattatg ttgcaatcga agcctattat agagaaactg tcgataaaat
      841 taatagagtt aacagaattg atagcaataa tcctgatatt tttatttcaa cttttgataa
      901 tattggtaaa gatatttcaa caggagcaga gcttatgaca aatctaaatg ttacaaaatg
      961 gtataatttg aatatttctg gaacaggata ttattatgaa attattgctg gctcaaacaa
     1021 ttataactca aacaattcta ttcaatggaa tgctagaatt aataatacta taaaaattaa
     1081 aaaaacagga acaggaatac aattaggtgc ttttttaaat ggaccaaggt tgagcgctca
     1141 aggcaaatct agtgcaagct gggcagcagg tggtggagta agacaagact ttttagacag
     1201 acaactttct gtaagtctta atcttagaaa tattttccgc actatgaaaa tggagcgtat
     1261 ttctgaaact gacagcttta gccaacattt tttgagacag cctcgtgggc ctattgttac
     1321 cttgtcggtt acatataaaa tcaacgattt taagagcaga agagaaaaag gatttactaa
     1381 cgaagaacct ggtagaggtg gtggagatga cgaaggaatg tagtttgtta aaaatatttg
     1441 ctaaatcttg aaaaattatt ttgtttttta aaatagagtg tgtatatttg caccccaaaa
     1501 gtaatagatt ttaaatttta aagaaatgaa aaaaggaata catccagaaa aatatcgctt
     1561 agtggcattt aaagatatgt caaatgataa cgtatttatc acaaaatccg ctgcaaatac
     1621 aaaagaaact attgaaatag acggagttga atatccatta tataaattag agatttctaa
     1681 cacttctcat cctttctaca caggaaagat gaaacttgtt gacacagcag gacgtgttga
     1741 taaattccgt aatcgttata aagaccattt tgaaagaaaa aatcaaaaga aagcttaatt
     1801 tataagtata tataacaatg ttttggaagc ctcgttaatt atttagcgag gctttttttc
     1861 ggtgttttat aagcatccaa ctggtgacct gagtttataa agttaaaagt tcataaagtt
     1921 cataaagttc ataaagtcga ttttataaca aaatgtgtca acttttctta ggacgcgaca
     1981 agcactttta actttacctt cggtgagctc gtaaactcgg ttcaactttt aacttttaac
     2041 tgatttattt tctatctttg taaaaaaaat aaataatgaa tttcatatta tcagatttta
     2101 ataaacatca aaaatttcta cctcttacct ttacaagagc tttggctgat tatagaatag
     2161 gaattttaac tcttaaagaa aaatgggaat tttacctaaa tcaaaaggtt tctgttctta
     2221 cacaagagta tttgcaagct aagtacagtc ttaaaataga aaatgaaaat atagtaatta
     2281 atgctcaaat aattccaaat ccagagctag taggcgaaat tttaaagtta aaagacgctt
     2341 gcttagtaaa agacgaaaat attattgctt attcttgctc ggataatctt gaagacattc
     2401 caaaaactaa aattgagttt aaaggcgaag ttttaataat taattccaat actgatgttt
     2461 ttacttttaa tggagaactg attaagcaag attttgacat tatcactaaa ggtcgaaaaa
     2521 gtcaaaaatt aagttctaca aatacagttt ttggaaaata tccagttttt gttgaagaag
     2581 gctgttttgt ggaatgtgca agcataaaca ctaacgatgg acctgtatat attggcaaga
     2641 atgcaaaaat aatggaaggt tctcatatta gaggtgcatt tgcaatttgc gaaaattctg
     2701 aacttaaaat gggtgctaaa atttatggac caacaacgat aggaccaagc tgtaaagttg
     2761 gtggagaaat taataattct gtattttttg cacattcaaa caaagctcac gatggctttt
     2821 taggaaattc tgtaattggc gaatggtgca atctcggtgc tgatagcaat aattcaaatt
     2881 taaaaaataa ttatgctatt gttaaacttt gggattacga aaaagaaaga tttgtaagca
     2941 caggcttgca gttttgtgga ttgataatgg gcgatcattc aaaatgcgga ataaatacca
     3001 tgtttaacac aggaacagta gttggagtgt cggcaaatat ttttggagca ggctttccac
     3061 gaaactttat tccttcgttt tcttggggag gacatgccgg tttgtcagta tataagacag
     3121 aaactgcttt cgaagttatg acaaaagtga tggaaaggag aaatgtacaa ctaacagaaa
     3181 tagataagca aattctggct catgtctttg atataacagc aaagtatagg aatttttaaa
     3241 caaaattatt tcttttatgt tttatatggc acaagtattg cctattaagg atacttatgt
     3301 tttattataa acttatgaca atttgacatt atgacaaaaa ctttagataa cgaattttta
     3361 aaaataatta tgggagcagg aaaagaagaa gcggaattta tccctatagt aaccgaattt
     3421 aacgaggagg aagatgatac taatcaccct gagactttgc ctattttgcc tttgcgaaat
     3481 actgtattgt ttcctggtgt ggtaattcca ctaactattg gtcgaagcaa gtcttttaaa
     3541 cttattaaag atattcacaa aaccactaag ttttttggag cagtagctca aaaagaatta
     3601 gaaattgaag acccaaaacc agatgatatt tataaagtag ggactttagc agagattttg
     3661 aaagttatag aaatgcctga tgacacaatt tctgttatta ttcaagggaa aaaacgtttt
     3721 aacataaatc aatatttaac agacgaacca tatcacatgg ctacggtaag ttatattgca
     3781 gaagataaaa ctattgaaag taaaagaagt tttcaagcaa tagtttcttc tttgaaagat
     3841 atgtcgctaa aaattattaa acttagtgcg agcatacctc aagaagcttc ttttgctatt
     3901 aaaaatattg ataatccaag ttttttaata aactttattt gctcaaattc agatattgaa
     3961 actgctgaaa agcaaaagct tttaacagaa aacagtttgt ataaaagagc tgaaaaactt
     4021 ttaggaatac ttgtcaatca agtgcaaatg ttagaattga aaaacgatat tcaaataaaa
     4081 gttaaaaaag aaattgacaa gcaacaaaaa gaatatcttc ttcatcagca aataaaaact
     4141 attcagcaag aattgggtgg tggaccagct gaacaagaaa ttaaagaatt agaagaaaaa
     4201 gcaaaatcaa aagtttggaa aaaagaagta aaagagcatt ttgaaagaga attgaaaaaa
     4261 ctacaaactt taaatactgc ttctgcggaa ttttctgtgc agttaaatta tttgcatact
     4321 ttgctcgact tgccttggaa tgaatatact gttgataatt ttgatttaaa acatgctcaa
     4381 aaagttttag acgaagacca ttttggcttg gaaaaagtta aagaaagaat tattgaacat
     4441 ttagcagttt taaaattaaa aggcgactta aaagctccaa ttctttgttt gtacggacct
     4501 ccaggagtag gaaaaacttc tttaggtaaa agtgttgcca gagctttggg caaaaaatat
     4561 attagaatgt ctttaggtgg agtgcatgac gaagcagaaa ttcgtggaca tagaaaaaca
     4621 tatataggag cgatgccagg acgtattttg caaaatataa aaaaggcaaa atcttcaaat
     4681 cctgtgtttg tgcttgacga aattgataag gtaggtagcg actttaaagg cgacccttcc
     4741 tctgcacttt tagaagtttt agaccctgaa caaaatacag aatttcatga taattttgtt
     4801 gaaatagaat atgacttgtc aaaagttatg tttattgcaa cagcaaacaa tgttcaaact
     4861 ataagtcctg cacttcgtga cagaatggaa atgatagaaa ttagcggtta tattattgag
     4921 gaaaaaatac aaattgctaa gcgacatcta ataccaaaac aattagaaaa tcatggactc
     4981 aaagatttaa aacttaaaat agatgataaa gccttagtta aaataattga aggctatact
     5041 cgcgaatctg gagtaagagg tttagataaa aatatcgcta aaattattag aaaaatagct
     5101 ttaaaaattg gattaaatga agaagttcca aacaaaatta cagaagctga tatagtagaa
     5161 tacttagggc aagaaaaatt cctgaaagaa gaatatcaag attctgaaat tgcaggagta
     5221 gttacaggac ttgcttggac agcggttggt ggcgatattt tatatattga aacaagtttg
     5281 tcgaaaggca agggcgaatt gactattaca ggaaatcttg gagatgtgat gaaagaatct
     5341 gcaactattg cacttcaata tattaaagca aatgcagaag tctatgatat tgaccctaaa
     5401 cttttcgaaa cttataatat acatcttcat gttccagaag gggcaattcc aaaagacgga
     5461 ccttcggctg gtattacaat ggctacagca atagcttctg catttactca gcgtaaagtt
     5521 gcaaataaaa ttgcaatgac tggcgaaatt acacttagag gaaaagtttt acctgttggt
     5581 ggaattaaag aaaaaatatt agcagcaaaa cgtgcaggaa taaaagaaat tattctatca
     5641 aaagaaaacg aaaaagatat tacagaaata aaagatattt atatcaaagg tttaaaattc
     5701 cattatgttt ctaatatttc tgaagttctt gatattgctt tattaaaaca aaaggtgaga
     5761 aacgcaaaaa aattgtaatt ttgcacaaca tttaaaaaat gcaaagaaaa aacatagatt
     5821 taatggcacc agtgggttct tacgagtcgc ttatggcagc cattcaagct ggagcaaact
     5881 ctgtttattt tggaattgga aatttaaaca tgagatcacg ttcttcaatt aattttactt
     5941 ttgaagattt aagaaaaatt gtaaacattt gcaatgaagc aggcgtaaac acttatttaa
     6001 ctgtaaatac tgttatttac gatggcgaat tagacacaat gcgacagata attgatgctg
     6061 ctgccgaagt gaaagttagt gcagttattg tttctgatgt tgctgtaatg aaatatgcac
     6121 gcgaaaaagg agtggaggtt catgcttcaa cacaattaaa tattgcaaat attgaagcgg
     6181 ttgagtttta ttctcagttt tgcgatgtta tggttactgc tcgtgaactt agccttgacc
     6241 aagttgcaga tattacctat caaatcaaac aaagaaatat aaaaggacct aatggcaaac
     6301 ttgtagaaat cgaagtattt tgtcatggag ctttatgtat ggcaatttca ggaaaatgtt
     6361 atatgagttt gcacgaattt catcactcag caaatcgtgg tgcatgctta caaaattgca
     6421 gaagaactta tattttgaaa gacaaagaat ctgatactga aattgaggtt gataatgaat
     6481 atttaatgtc gccaaaagat ttatgtacaa tttcttttat tgataaaatt attgatgcag
     6541 gtgtaagtgt tttaaaaatt gaaggaagag ctcgttctgc tgaatatgtt aaaacagttg
     6601 ttgaatgtta tgaccaagca ataaaatcgg tattggacaa tacttataat caagaaaaaa
     6661 ttgatgaatg gacaaaaaga ttaagtacag tttttaatcg tggattttgg aatggatatt
     6721 atcttggtca aagacttgga gaatgggcaa aacagcatgg ttctaaagca acaaaaacca
     6781 aagtgttttt aggaaagatt ttgaactatt actcaaatat aggagtagct gaaattaaat
     6841 tagaaactga tgaagaatta ctcgtaagtg atgaaatagt aattttagga ccaaccacag
     6901 gagttgtaca gcaatttgta gaagaaattc attttgaaca aaaatctgta aaaaaagtaa
     6961 agcaaggcga tttgttttcc attaaaactg atgaattggt taggagaggg gataaactct
     7021 acaaattaga agatacagaa gaacttaaaa aacgaaatag agaacgtaaa tatcaaataa
     7081 ataatgatga gtaataataa tattccagaa agagttgtaa agtttataaa tcaacatcat
     7141 gttgctacta tagcgacttg tgtagattct gaatcttggt gttttaattg tttttatgca
     7201 tttgtagaaa gcgaaggggc tttgattttt acatcgagta ctgatactaa gcatataaaa
     7261 atggtggaga aaaaccctat agtctctggc tcaatagttt tagaaacaaa aattgttggc
     7321 aaaattagag gtctgcagtt tgttggagaa atgcaaaaag tcgaaaatac agaaaattca
     7381 aaatatagac taaaatattt ggcaaaattc ccttttgcag tcattgtaaa ctcagattta
     7441 tggatactaa aacttaccga actaaaaatg acagacaata gactaggatt tgggacaaaa
     7501 ctagaatgga aaaaatcagt tgaaagttaa aagcacacgc tcattgagcg aagtcgaaat
     7561 gagcgtgtgc ttttaactga ctaaaaccca ctaacagtta tccattgtcc ttttgtccta
     7621 gcaaaattag aatcatcata cattgtttta caaacagttg caggcaagta atattttcct
     7681 tcataagcag ctgtaagcat tactctatat gtgtatgtgt atttgttata aatattaaaa
     7741 tatgtcaata ctctgtcatc tctaatatct tggtaagtgt agtaggagtc atctcctaat
     7801 tgtgtagtaa acattctaga gtttataatt tcccaaccag atggaaatac ttgagacaat
     7861 gcaacattat ccaaactagc taagttgctg acattagaaa tagtaacagt tgcaacaaag
     7921 tcggttcctt gtgggatatt agtaatatca ataggattac ctgtcaaact tgtgtattta
     7981 atactcatat ttatatttga cgaaccagct gtttcatgtt caatttctgg aatacctttt
     8041 ttaataattc ttacaaaata agaattgttt tcagttgggt ttaatacaaa attatttttt
     8101 tcattttcaa aaatctttaa ttttgtctta tatacaggct tatttgtttt tacactatac
     8161 ttggtaccat taaatgtgta attacattca ataatctctg aaaccgccca ttttttcaca
     8221 tatttagccg aagctaatag ggcataagca gatgtttgag tagaagcata ttgatttgaa
     8281 cccataaact cagcaatgtc ttttaaaacc ataaatgctt tttctttttc tccaagttct
     8341 atcaatgttt ctaaaatcat tgctttgtcg cgaatacctg aaccaaaagt tcctgatagt
     8401 tcgtaatacg aatttacatt agtatttaat ttagcaatca tcgattttgc aatgcttgta
     8461 tttccaatta aagcataagc acttgctaaa cgccaagctg tagcagattg aattttcgat
     8521 tcttttaatc tattcattgc cgatctatca gcttgttttg ctaaagctaa agtatataaa
     8581 cgataagctt gagttaattg agatgtattg ccgtcatcaa cccatttaga agcttttcgc
     8641 ttttgatatt ttaaccaatt ggatttaaca cttgaattta cagtatatcc cgatttttca
     8701 gcttctaaca taaaatgtcc ggcatagttt gttgcccatt ccgatacttc gtttaatcct
     8761 gcccaataag caaaaccacc acttgctgtt tggtacttat tcaatttgtt taagcaagca
     8821 ttgatatttg tctgtatttc agccttttgc tctgcggtta attccataaa agcatctaaa
     8881 tataattgtg gaaatacagc agaaacaatt tgttctaaac atccgtgagg ataagttttt
     8941 aagaaattta atctgtattc taaattcata ggtggaatat tgtaaatttc taagtaagct
     9001 tcgttgcttc ctgtaattcc atcagcatca aaagtaatat ttacttcgtt ttgtccactc
     9061 atttcatcta taatttcagc aacttttgta ttaacatgct tcacatctaa ttcaacatta
     9121 tatttagaca catgagatcc gctagttgca ataatttcaa ttttagcaat tcctgttttt
     9181 gtattagact ttaagctaaa attatgaatt ttttcgcctt cttttataaa attaacagta
     9241 gcagagcttg agcctacaac ttctagcatt tcgttagttt ttattgttaa tttaacgttt
     9301 ttaacgtcag gtttcatagc aaacactgtt acaggtaatt taaagctttc gttaactcca
     9361 agtcggcgag gagcggttcc taaaatcatt aaaggtttct ttacaggagt ggttttttct
     9421 gcagaaccaa aggcatttcc ttcggcagca actaccattg ttctaacaga acctatgtat
     9481 tgtggtaatt gaatttcgtg agtttttgtt tcaccttttt taagctcgaa aggaccgata
     9541 tattttacca taggtctaaa tctatttgct tgagctaaat cttcgggtgc aacttcatca
     9601 tcgccaccac caatactcaa cattttgcca gcatcaattt taaaagcacc tataacatag
     9661 ttgaaaatat cccaagttct aacacctaaa gcttccttac tgtagaaata agcccctgga
     9721 tttggagttt taaattttgt taaatttagt aaaccttcgt caaccattgc tagggtatag
     9781 ctcataggtt ttccatcttt ttctttcact tgaattttta ccttactttc ggctaataat
     9841 tcattattca tcacaagttc aggattcaaa tgagtttctg gattttctac aaaaattgga
     9901 ataattccat acatacgcaa tggtctgtca tttttaattg tcctcaagtg ttcctgtaga
     9961 taaataatgt taacatacac attaggagcc atttcctgag tagcttcaaa ctcgactttt
    10021 agtttagaac cgtcagattt tacccaaaaa ctatttaaaa ctgtattggc attttctata
    10081 ctaactaaag catgacctgt atgtgctggg aattctattt ctacagtttc gcccacattg
    10141 tatttttctt ttgaagccga aaatttaagc atttcaacag agcctttgtc gctttcgcta
    10201 gcatacgagc ttgcataaat tttcagtgct gcaatatgtt ttcctttcaa gtcttttaca
    10261 gctattacat agtttccaga ttcgttaata ataaagccaa aatttgattt tccgtttttg
    10321 catttttgat tttctctaaa tattgcatta ttatagtttg ctgaaatata atcaacacca
    10381 tcgcgttgga aatcgtacca ccacgaataa tctaatttat acatagaaat ttctaaatct
    10441 ctattttcag caattagttt ttgatttaag tctaataaaa ctatatctaa attcaaattt
    10501 ttaccagttg gtaagctata agagtattca tctgattctt tcactttaac tcctacaaaa
    10561 gcgtcataag ggtagtaaac tgcagttgat tcatcaatac taaattctcc gcctttttca
    10621 aaaacttttg ttgttaaggt ggcatttaat acaccgggag cattttttat atcattaaat
    10681 ttcaaaagtt gtttaaattc accatctgca tttgtcttac cagaagcaat tgtttgacta
    10741 ttaaaattaa aattcgcagt ttggttttcg aaacaatagt cttcatattt ttgaaatgga
    10801 gtgtattttc tctctaggct cataactatt gtagtttgta aatcaactgc tggagcacca
    10861 tgtaaccatt tggcactaac catggcatta acagtactgt ttccttttaa gtaattttta
    10921 tctaagccta attcaacact taatctatta ggacgtattg tttctaccat tagagatttt
    10981 gtaaacactt taccgccaat aataaatttc gcattataat aacctgtagg gtcgtttggt
    11041 tcagtcttaa atttaaagac atggaatcct tttttatttt tattttgttt tcctgtataa
    11101 actaactgtc ctttaggatt tttaacttct aacactattg gatgaccttc tgggataggg
    11161 ttgtaggtct cgtgcaaaat aaaacctaaa tatattgaat cgcctggtcg ccatacgcca
    11221 cgttctccat aaataaatgc ttttgtacct tcttgactag aatctcccca cacatcaaat
    11281 tcgcttgtgc ttaaagagtt gtagtattct aattttaaat atgatttttg attattaaaa
    11341 cttgcaacta taaaatatgg tttaagattg ttgctaataa taagttcagc ttgtccattg
    11401 gtattagttg tagcttttgc aatcacttgt tgttgataat cataagctaa tacttcagca
    11461 ccagttattg gttcggcagt taaaatattt gtaacaaaag caaaaacttt attatcgctt
    11521 ccaatctttg aaacaattcc tatattagtt gctaaaatgt tgaatttcaa cgcttttcta
    11581 tacccatagt attcagattt acatggatcg tcataagaat tttcataatc atcataatag
    11641 taatcatcgt agtaataata ttcatcgtcg taatagtagt cagatgagta atcactataa
    11701 taataatctg taaaccattt ccattcactg tctgcatcaa ttttgttaag attatcatct
    11761 tctttattat ccttatttgt actgttatca catggataaa tagcattttc tttaataaat
    11821 ccaatttcta ttctataaat tgctcctggg tctggttcta ttaagtcaga tagatttaaa
    11881 aaatatgtat tccattcacc aatatcgtca atatcagctt gttctagact taatgtttga
    11941 gttttaacaa ctcttccaac tctatttaaa gaatatgatt catcaagcga attatcttgt
    12001 aaaaactgca atatattgtt ttggaatatc ttaattactc taatatgaac agtttttata
    12061 ttaacagctt ggaaactaat cacttgtcct tgagaagaag taggcaatat tacaccatca
    12121 ttatcaaaag ctaattctgg ttttcgcatt ttaaagttta attccttaga tacaggttca
    12181 tctaagtact tatcatgaat attttttact cctgatatat ttaataaata gtcattatct
    12241 aatctgtcgc tagggataat tttcacattg ttgtcaacaa taatatattt taaattgtaa
    12301 acatcttcaa tatcaactat tccttcaagt aattggtctt cttttacggg gtcgctaaaa
    12361 attatattta cgtattgttc tggatattga tttacttgaa tatcaagcac tttgaaaaca
    12421 cctaactctg gaatttctat ttcacgtgaa aatttagaat ttgaatttat ttcttttcca
    12481 ttgcaagtta tttttaatgt ttgagaatta tttgttctag tgatatcttt aattaaaaat
    12541 tcaaattcat tattatttga actttcaatt tctatatcta attttttgcc attaagttct
    12601 gcactaatac atttcttaat gttttcgaga ttttcggaat cagttaattt tattgttcct
    12661 ccaacatttt gttttgtaaa attatctctg tctgttgtaa taactttatc aatattcaaa
    12721 tctatgaatt gatgttttac ctgaacttta aaaacaaatt cttttaattc ttttggaaca
    12781 ctagaatcta atttccttaa atttagcttt acacaatagg cttgccctga ttgtaacggc
    12841 ttttcgggtt taaactctat aatattattc tctttccaag taagttcgcc ttttacttta
    12901 ggttctatag ttaatatttt agttttttga gactttactt tataggcaaa ttcatcggtt
    12961 aattgtactg taattacatc attttttgat ataacaaagt ttgaatacgc tgctatataa
    13021 tctttgtaaa gacttgggtc agatagtttt acttttttgt ctgaaccgaa catgctaaca
    13081 atagcaaaaa taactactcc tagtaaaatt gcaccagaaa tccaatagaa aattcttgtt
    13141 ttattcatga taaatgtttt ttatttaata caaaaatagt tttattttta tatgacttta
    13201 tttttattta aaaattatat taacaatttt gacagctttg tttttaataa aattataaat
    13261 taagtttcag cttcttcttt agtaatttta atagttatac cagttttttc gctaaatccc
    13321 acagtaatag agtctccttt ttgaatttcg gcttttatta ttgtttctgc catagcgtct
    13381 tcaacatatt tttgcaaggc tctttttagt ggtctagcgc caaattttgg gtcaaatcct
    13441 ttttctgcaa cataattttt agctgctggc gtaagtttaa gattataacc caattcttta
    13501 actcgtttat atagtccttt taattcaata tcaataattt tgaaaatgtc ttctttagtt
    13561 aaagcattaa acattataac atcatcaata cgatttaaaa attctggagc aaaagtcttt
    13621 ttaagagcat tttcaattat agtttttgca cttgcatcgt aacttgcttt tttagcactt
    13681 gattcaaatc ccacaccacg tccatattca tttaattggc gagtaccaat atttgaagtc
    13741 attataagaa tagtgtttct gaaatcaatt cttcttccta agctatcggt aagtctgcct
    13801 tcgtcgagta cttgtaaaag aatgtgaaac acatcaggat gtgctttttc aatttcgtct
    13861 aataaaacaa tagagtaggg ctttcttctt actttttcgg ttagttgtcc gccttcttcg
    13921 tagccaacat atcctggagg agctccaact aagcgagaca ctgaaaattt ctccatatac
    13981 tcgctcatat ctattctaat taaagcatct tcgctatcga ataaatatct tgctaaaact
    14041 tttgccaatt gtgtttttcc aacacctgta ggtcctaaga atataaatgt tcctataggt
    14101 ttatttggat cttttaatcc agctctattt ctttgaattg cttgcacaat ttttttaaca
    14161 gcttcatctt gtccaataac gcttccttta atatcttcgt ccatattgag taaacgatta
    14221 ccttctgatt gtgcaatacg ttttacaggc acaccagtca tcatggcaat tacttcagca
    14281 atatcatcat cgttaacaat ttcgcgaact tcatttagtt ctttaagcca attttcttta
    14341 gctaattcta tgtgtctttc tattccacgt tcttggtcgc gcatagcagc agctttttca
    14401 aattgttgtt gtgtaactgc tttaattttc tcgtctttaa ttttttgtaa atcttcttct
    14461 agtttaataa ttttatttgg aatagaagca gaagatatat gctttctaga accagcttca
    14521 tccattgcgt caatagcttt atcgggcaaa gctctgtcgc taatatagcg agtagttaat
    14581 tttacgcagg cttctaaagc tttgtctgta taagaaactt tatgatgatc ttcgtattta
    14641 tctttaattt tctttaaaat atctaatgtt tcttctgctg ttggcggttc tacaattatt
    14701 ttttggaaac gtctttctaa agctccatct ttttctatac ttttacgata ttcatctaaa
    14761 gttgtggcac caatacattg aatttcgcca cgtgctaaag caggttttaa aatattagca
    14821 gcatctaaag agccaggaag accgccagca ccaacaatag tgtgaatttc atcaataaat
    14881 aaaataacat caggagctgc tatcaattca tctaaaatgg ctttcattct ttcttcgaac
    14941 tgacctctat attttgtgcc agctacaatt gttgccatat caagaataac cactctttta
    15001 tcaagtaata ggtgaggaac tcgtttttga acaatcttag ttgctagtcc ttctacaatt
    15061 gccgacttac caactccagg ctcaccaatt aaaacaggat tgttcttttt gcgtcttgac
    15121 aagatttgag caattctttc tatttcagta tctcttccaa caatagtgtc aactttgcca
    15181 tctattactg cttgagttaa gtctattcca aaactatcta acataggtgt tgatgatttt
    15241 gatgacgtag atgattttct tggactagaa ctagacctag aaggtctata agaatcttct
    15301 tcatcatcat cgtcatcatt ttctctggaa cttgaaacat cagaaaaatc atctaattcc
    15361 atagtcattt taa
//
LOCUS       DAOPNX010000064        15340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_4593_length_15340_cov_6.755708, whole genome shotgun sequence.
ACCESSION   DAOPNX010000064 DAOPNX010000000
VERSION     DAOPNX010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15340
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4593_length_15340_cov_6.755708"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..768
                     /locus_tag="PKN53_07780"
     CDS             226..768
                     /locus_tag="PKN53_07780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOD88884.1"
                     /translation="MDDNEKLIRQCQKRSKKAFDELYKTYSPIIFGICLRYTRNKTEA
                     EDLLQNCFLKILNKIDDYEFKGSFEGWIKRLTINAAINFFHEKRRSLVEPVDSYEPQS
                     NNFDYDVVSQMSAKEIMEVVNELSIGYKTVFNLYVIEGYKHTEIAEMLQISEATSRSQ
                     LKRAREILQELIKKRLNGEI"
     gene            755..2404
                     /locus_tag="PKN53_07785"
     CDS             755..2404
                     /locus_tag="PKN53_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOD88885.1"
                     /translation="MEKFDEILKSKLENFQETPSEEVFLKIKAQYPKPKGFLKTYKYH
                     IAAGLAIIALSLATIYIVNNQTKPIETTNQTTNTIIEENKQIITNQNNINSENQILNN
                     SQNNQIPIIKQNDLVINENKLISVVNENKNVVNEKTISQKVFKTNDTVICGKSIEIPF
                     GAKTNLLQLPKDIIVSKSDLGININSQNFGKYTIYYNDISNVFRDSITINFKNNDFAK
                     VDFSNENICPNEDLIINIKNSNSKLVWGDTNFKVLKKSSSTYIIKNLKSGENLISFNL
                     EENGCTQQISQTINVLKSPKYKIQTESEVCSKANGKLLILSDDKNINSFTLNNEITNS
                     TGRFTNLNAGIYFVKIGFANSCYIADTVFISDSLNISPYFVSDRDLVNKNKYQFINYT
                     RIDDSGFERNNQVNFIWKINDEEKSELDNFSYEFVKGGTYKIELFASLSETCQKSYSE
                     TIEISETNLRIPNIFTPNGDGLNDEFVVCYDGELVKFKIDITNKNGEVIFQSTDINKS
                     WDGKINGNDHAADGVYYYNINGEDNFGNKIERRGALRLARH"
     gene            complement(2469..4619)
                     /locus_tag="PKN53_07790"
     CDS             complement(2469..4619)
                     /locus_tag="PKN53_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOD88886.1"
                     /translation="MKNYQTEQIRNIALVGNSGSGKTILAESMLQIGGVINRKGDIAS
                     KNTVSDYRLIEQENGNSLYSTVMYTEINNTKINILDNPGMDDFVGGLVSSLFPADIAV
                     MLINTANGIEVGTEIHGRYVEKAEKPMVIVMNHLDHEKTNFEKVFESIHETFGGNAVL
                     VQFPVNAGVGFNSIIDVVTNKMYTIKGTEIVISDIPAEHADHAEEVKTQLIEKAAEAD
                     ESLMELFFENDTLTEEEMMRGIAAGLPQRGIFPVFCINARNDLGVKRLMEFIANVCPA
                     PNKLPAHVNKEGTEIKCDPNGKPSLFVFKTSIEEHIGEVCYFKVLSGTLTESIDMYNP
                     KTNTKERLSTLFVNAGKNRVKVDKMYAGDIGATVKLKGTKTGATLVAPGEDWEMPAIK
                     WPNPKYRVAIKAVTEGDDEKLGEALNKLKDDDQTILVEYSKELKQLIVQGQGEYHLNI
                     LKWHLDNQFKIPTEFSRPKIEYRETITKTAFADYRHKKQSGGAGQFGEVHMIIEPYTE
                     GMPDPVMYKIDGKEYKISVRAKDEYDLEWGGKLVYYNCIVGGSIDARFLPAILKGIME
                     KMEEGPLTGSYARDIRVCIVDGKMHPVDSNEISFKLAGRNAFSNAFKKAGPKIMEPIY
                     NLEVLVPSDRMGDVMSDLQGRRAVVMGMSSEKGFEKIMARVPYAEMATYSTALRSNTG
                     GRAMYEMSFHEYQQVPPDVQEQLLKEYEAEDDDE"
     gene            4802..6244
                     /locus_tag="PKN53_07795"
     CDS             4802..6244
                     /locus_tag="PKN53_07795"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOD88887.1"
                     /translation="MRKKFIINLAFLLLLNLLIKPFWIFGVERSVQNIVGSAEFGFYF
                     SLFNFSLLLNIILDLGITNFNNRNISQNPQLLRKHVSNIVSLKLILALVYAALTLGAA
                     LIIGYDSRQLGILLILILNQFLSSLILYLRSNISGLQLFKTDSLISVLDRFIMIVVCS
                     IIIWGNVTDKPMKIEWFVLTQTFAYLTTAIVTFSIVVYKAKFFKLNFDFRFFRVFLRQ
                     SYPYALLILLMAFYNRIDSVMLERLLPDGKHQAGIYAQAYRILEAASMLPYLFSILLL
                     PMFSRMIKKKENITELAKLSFSMILVPAIFLVCVCYFFSEQIMDLMYWENVEASSKTL
                     SFLMIGFIGISTTYIFGTLLTANGSMKQLNTMAFVGMILNIALNLILIPRLHVIGSAI
                     AGMITQLFTGFSQLILAKYVFKFKINYKLILSIVIFVAIVIVSTQLLSKLNMNWITII
                     VIIGLISLATSFATRLFKIKDIYKLLTERE"
     gene            6362..7330
                     /locus_tag="PKN53_07800"
     CDS             6362..7330
                     /locus_tag="PKN53_07800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD88888.1"
                     /translation="MVKNNIELSIVLPVYNEEKNIDLLYTKLISSVEQLKCAYEIIFV
                     NDGSIDNSLEIIINLAKNNPNIKYIDFSRNFGHQIAIYAGMENSKGNYVLTMDSDLQD
                     PPEEIINLYNKVKQGFDIVHARRKQRKGETIFKKLTAKIYYRILRKITKCDIPVDVGD
                     FRIISKKVVDILKQMPEQEKYLRGQIAWVGFKQTFIEYDRDQRAEGKTKYSTSKMTKL
                     ALDGITSFSDFPLRFATYLGFFVSIISFVLMLWVLYKRLIVKEYVQGWTSIILSVLFM
                     GGITLITIGIIGEYISRMGNNVRKRPLYIINKTNLDNEEEDKEEAN"
     gene            7299..9392
                     /locus_tag="PKN53_07805"
     CDS             7299..9392
                     /locus_tag="PKN53_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88889.1"
                     /translation="MRKKIKKKQIKILSIVLLLVAIPCIICILGNKFENKFKNKEIVV
                     SASIKPISFFLSFENNNYLNETHKITNLKFLSGRKSVIIDKNGGQSPIITIPIPDHAD
                     MSFVNINCNFWLSPDTSFINSLFVFTIIDTNNTVLYQQEREIKAENLNSINWYNFNEN
                     FNIQTQYLNSQHFLQMFIRNNLEHSSNLYFDDLEIVFEKENHKTKALLVDFEDDKFQK
                     TSSKYCFSGNFSTYASGTNDYSEQVVLDFENINYKNLDKILLNLNYLSETEDLDAVFI
                     VSICDTSDTEIFCSELELHKDKNFRPEKWENISTEIIMPKSVVDKGSFIKFYLWNKNS
                     NTVFVDDVYLIFKEFSIEYGNKPTAHNFILNPNFQIKKNQAPYNFYYLNREKKFANKE
                     KELSKFFTDFNKILIGNFKDNTNLDQILAIGTVDIMFYDFENQTKIKHDFENLNNLQF
                     FTDGQNVFSYQEKAKKLGLHKINFSTNSLDLETEIVYNSENKISEIFSINDSIISIFD
                     NFGNVYDYLKKDGKYSLIKTSKLISCTKGNLKLLKSNFYNEETEEVLIIFQETEKVKY
                     EFFTYCINENMWKHSTNCSNTSIQYFDKLDLQSEYFLIDYNGDGVFELLKFNRTNKFN
                     LKLLSFDFRSYKILYEIDFKGFENSQNPKYYEKLKILTGNFADIRGSEVIIFQDNVNK
                     VDWLNQKTEIYSFNK"
     gene            9394..11298
                     /locus_tag="PKN53_07810"
     CDS             9394..11298
                     /locus_tag="PKN53_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88890.1"
                     /translation="MKIYKIYILFLITLLFVSCGSDKKKTCDYSVSDIDFLANYDSNE
                     LDLDYENALSGFTIPSINQTESGYFNFNFKLTNNSNKEKEYFYKIYYQNESYKIQECD
                     SKNKAQQAQYVEENFYGSWEDTNIGFKSLGKISPSEFIVLTDSFRIVGNPRNEEKYFF
                     YGENDRWKRNPRVGEYSFMLVIVDEENFEQIPEYIKHINLLKDSANFVSPYYYFLYGE
                     GKDIKNCFVKLEEKALKVTAKPNLGGGIYVNHYMFDETIEDLDKYQSCNCNNSSMEEK
                     AHFEQFLHYIDESSRLHNVPVIKDVLNEGFTNEEYNWLSAFYKIEDRITVLPQLAKYP
                     CQTIKSDSVNNKIIICNPASTYGNWQKQNVGVIARHGFVYGKYTVKAKLTSLLNDDNV
                     WNGITNAIWLINQKGRGNETGWNLRRSCNKNGYMKTYWGGRNDERVEKVDYSEIDFEI
                     LKTTPYCPRTKTKLTKSDQAMPDKIDSWNIELPENIKKDEGKIAVCCTNWDMACWEPK
                     NFSTGCHKIKYNNKTFYLHRWDNTYRAITLKYLASNKELFGGDYYYYQIDWQPDKIIW
                     RIGPEKDKLYEVGYVDQTVTMVPNNQMTLIITQEFHNTNWWPGSPYGQENIPFPAKDY
                     VGEIYEITIE"
     gene            11491..12027
                     /locus_tag="PKN53_07815"
     CDS             11491..12027
                     /locus_tag="PKN53_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88891.1"
                     /translation="MKTLFIFLLMFVANIAVSQVVSDTLVKNLGKVNSDFVYDEDNLV
                     LIPNTNIKMQAPEYFLISENIPGLVHPGSSATVQVQEITGTSYIMISQAMTDKHFESQ
                     GVRLISTNEVTMQDGKGGILYLVEFEASGIIYERLMLFAGDYNNTIWINANYPKSAKK
                     LLQDILTQSLLTAQYTKQ"
     gene            12030..>15340
                     /locus_tag="PKN53_07820"
     CDS             12030..>15340
                     /locus_tag="PKN53_07820"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HOD88892.1"
                     /translation="MVRKFLIILAIFVFCGCGLTKAQNILVHEIPYSTHHQDMWGVGE
                     AFSLDFDYDLFNIAVQDGFTVGEITEVLGQQFGVMVDMGFYIFLRSTFSIHGFTLGSV
                     DVDYPVRITWDFPDDYTFDHGETITINSSYDVLDGWALDTHFPTAGVIALDLEYGFGL
                     HLDIIVCLFSCETIPIIPTINIPVVPYSTDPLPHDSIAIFYLNGQTGEVAYPCLDPVT
                     GLPNICNDDILPIVIPDWFGIGLTGEIDIPYVVTEDSYNPNTQCLHAHGSDEWLWFNL
                     DILEFLSFIAGFIPPPNGPAIQQAIGIINGQTIEYEIIAGVTATIEYFILHLDLHMSS
                     HMTQDFAFCPTILATLRFPIDLPYSETDPSNGNAVVAEGVNDTITMIVNNDLHITYPC
                     YEWDSMQVYDITYNIISTFSNHTWDSIAFDFILEAIFFQISIPTSFLPVAKMPEFCLP
                     QIQANNADLGTYQYINACAPAITAPAASISSVFDESTETKGGSVSQTKDLTFCFPTNC
                     EPLISESFPLGSIPLTWFDQTWLLEGFVQDTVFPGTWLFPLPELDIEITAQDVICYGD
                     STGVITVEALHSTGPYTFEYSWGNVNTHLDPIDEINVPSGYYYVTLTDVYGCQVEGEI
                     NVADENPPILSSLYADDVLCHGEATGVLYSHVSGGVPPYTWNWQPSGRTEQNPSGVFA
                     GWHYLTITDAVGCEHYDSVFVDEPDAPLTMDYISGNVSCYGYYDGYIDVTPEGGTPPY
                     YYQWSNGQMTQDLINIPAGQYITTITDANGCTITETFDITQPDPLELTTYTQDVLCYG
                     ENTGSVDLVVTGGTPPYSYTWSNNSNNEDLLDLFYGIYIVTVVDANNCTEYTMVQIQQ
                     PALPLHGDITPTHVRCFGEGNGVADLNVFGGTPPYYFSWSTGAISEDIENLIPGIYSV
                     TINDEHGCETADTVQIFQPDAPIVGTISGNDITCNGGNDGNIYITVQGGRPPYTFEWS
                     NGSWEQNLINVQAGSYTVTVTDQSHCHYEMNIELTQPEPFYAQIMDDLTICYGESVEI
                     GIGIIEGGTPPYTIVWSNGDQGMSTTVSPTETTTYTVQIVDAIFCESQTAEITVNVHK
                     PLSLEVSASATKVCPG"
BASE COUNT         5618 a         2160 c         2423 g         5139 t
ORIGIN      
        1 aagtttatga atggacagat tgggaaactt atgaagcagg ttgggacgga aatattaatg
       61 gaggagcaaa agcatctcca ggtgtgtatt actatgagat taaggctact ggttttgatg
      121 gaaatatcta cgaagaacaa ggaattttgc atttaatgcg agaataatta aaaatccatg
      181 caacgttttg cactcgagaa ctgtctatat agaagaaaat aattaatgga cgataatgaa
      241 aaacttataa ggcaatgtca aaaaagatca aaaaaagctt ttgatgaact ttataagacg
      301 tattcgccaa ttatttttgg aatttgtttg agatatacta gaaacaaaac agaggcagaa
      361 gatttgttgc aaaattgttt cttaaaaatc ctaaacaaaa ttgatgatta tgagtttaaa
      421 ggttcttttg aaggttggat aaaaaggctt acaataaatg ctgcaattaa tttctttcac
      481 gaaaaaagaa gaagtttagt tgaacctgtt gatagttacg agccacaaag taataatttt
      541 gattatgatg tggtaagtca aatgagcgcg aaagaaatta tggaagttgt aaacgaatta
      601 tcaataggtt ataaaactgt ttttaaccta tatgtaatcg aaggatataa gcacacagaa
      661 attgcagaaa tgttgcaaat ttcagaagca acaagtcgat cgcagctaaa aagagccaga
      721 gaaatcttac aagaacttat taaaaaaaga ttgaatggag aaatttgatg aaatattaaa
      781 aagtaaatta gaaaactttc aggagactcc ctccgaagaa gtgtttttga aaattaaggc
      841 tcaatatcca aagccaaaag gatttttgaa aacatataaa tatcatattg ctgcaggttt
      901 ggctataata gcattaagcc ttgcaacaat atacattgta aataatcaaa caaaaccaat
      961 cgaaactact aaccaaacaa caaacactat tattgaagaa aataaacaaa taataactaa
     1021 tcaaaataat attaattcag aaaatcagat tttaaataat tctcaaaata atcaaatacc
     1081 tataatcaaa caaaatgatt tagtaatcaa tgagaataaa ttaataagtg ttgttaatga
     1141 aaataaaaat gtcgtaaatg aaaaaacaat aagtcaaaaa gtatttaaaa ctaatgatac
     1201 tgtaatttgt ggaaaatcaa tagaaattcc ttttggagca aaaaccaatt tgttgcaatt
     1261 accaaaagat ataatagtaa gtaaatctga tttagggata aatataaatt cgcaaaattt
     1321 tggtaaatat accatatatt ataatgatat atcaaatgtt tttagagata gtattactat
     1381 aaattttaaa aacaatgatt ttgcaaaagt tgatttttct aacgaaaata tctgtcctaa
     1441 cgaagatttg attataaaca ttaaaaattc taattctaaa cttgtttggg gtgatactaa
     1501 ttttaaagtc ttgaaaaaat caagttcaac ttatattatt aaaaatctaa aatcaggaga
     1561 aaacttaatt tcgttcaact tagaagaaaa tggttgtact cagcaaatat cacaaacaat
     1621 aaatgtttta aaatcgccaa aatacaaaat acaaaccgaa tcagaagtat gctctaaggc
     1681 aaatggaaaa ttgttaatat taagtgatga caaaaatata aattcattca cattaaacaa
     1741 cgaaattaca aattctacag gtagattcac aaatcttaat gcaggaattt actttgttaa
     1801 aattggcttt gcaaattctt gttatattgc agatacagta tttatttccg acagtttaaa
     1861 tattagtcca tattttgttt cggatagaga tttagtaaat aaaaacaaat atcaatttat
     1921 taattatact agaattgatg atagcggttt tgagcgaaat aatcaagtta actttatctg
     1981 gaaaataaat gatgaggaaa aatcagagct agataatttt agttatgagt ttgtaaaagg
     2041 tggtacatat aaaatagaat tgtttgcaag tttaagtgaa acttgtcaaa aatcatattc
     2101 tgaaaccata gaaatatctg aaaccaatct tagaatacca aatattttta caccaaatgg
     2161 agacggatta aatgatgagt ttgtagtgtg ctatgatgga gaattggtta agtttaaaat
     2221 agatattact aacaaaaatg gtgaagtaat tttccaaagt acagacatta ataagtcttg
     2281 ggacggaaaa ataaatggaa atgaccatgc tgctgatgga gtttattatt acaatataaa
     2341 tggtgaggat aattttggaa ataaaattga aagaagagga gctttgcgtc ttgcaaggca
     2401 ttaggagttt tatttcatag ttgtgtaaaa aaaagagagg tcgctccacc tctctttttc
     2461 tttttatatt attcgtcgtc gtcttcagct tcgtattctt taagcaattg ctcttgaaca
     2521 tctggtggta cttgttggta ttcgtggaac gacatttcat acattgcacg acctccggta
     2581 tttgatctaa gagcagtaga ataagttgcc atttctgcgt aaggaactct cgccataatt
     2641 ttttcaaagc ctttttcgct actcattccc attacaacag cacggcgacc ttgcaagtca
     2701 ctcatcacat cacccattct atcgctagga actaaaactt ctaaattgta aataggttcc
     2761 ataatttttg gaccagcctt tttaaatgca ttactaaacg cattacgtcc tgctaattta
     2821 aacgaaattt cgttagagtc aacagggtgc attttaccgt caacaataca aactctaata
     2881 tcacgagcat atgaacctgt tagtggacct tcttccattt tttccataat tccttttaga
     2941 atagctggta aaaatcttgc atcaatagaa ccacctacaa tgcagttgta atacactaat
     3001 tttccacccc attctaaatc gtattcgtct tttgctctta cagaaatttt atattctttt
     3061 ccatcaattt tatacatcac aggatcaggc attccttctg tgtaaggttc aataatcata
     3121 tgaacttcac caaattgtcc ggcacctccc gattgttttt tatgacgata gtcagcaaat
     3181 gctgtttttg taatagtttc tctgtattca atttttggtc tagaaaattc agttggaatt
     3241 ttaaattgat tgtctaaatg ccatttcaaa atatttaagt gatattcgcc ttgtccttga
     3301 actattaatt gtttcaactc ttttgaatat tcaactaaaa ttgtttggtc atcatctttt
     3361 aatttattta aggcttcgcc aagtttttcg tcatcacctt cggttactgc tttaatagca
     3421 actctatatt ttggattagg ccatttaata gctggcattt cccaatcttc acctggagct
     3481 actaaagttg ctcctgtttt tgttccttta agttttacag tagcaccaat atcaccagca
     3541 tacattttgt caactttaac tctgtttttt cctgcgttca caaacaaagt agaaagtctt
     3601 tctttagtat tggtttttgg attatacata tcaatacttt cggtaagagt accgctaaga
     3661 actttgaaat aacatacttc gccaatatgt tcttctatag aagttttgaa tacaaacaaa
     3721 gatggttttc cgtttgggtc gcatttaatt tctgttcctt ctttatttac atgagcaggt
     3781 aatttgttag gagcagggca tacgttagcg ataaactcca ttaaacgttt tacacctaaa
     3841 tcgtttctag catttataca aaatacaggg aaaattccac gttgaggaag accagcagct
     3901 attccacgca tcatttcttc ttctgttagt gtgtcatttt caaaaaacaa ttccattaaa
     3961 gactcatctg cttcagcagc tttttctatt aattgtgttt taacttcttc agcgtggtca
     4021 gcatgttcag caggaatgtc gctaattaca atttctgtgc cttttatagt gtacatttta
     4081 tttgtaacaa catcaattat tgaattgaaa cctacaccag catttacagg aaattgaact
     4141 aaaactgcat taccaccaaa tgtttcatga attgattcaa aaactttttc aaaatttgtt
     4201 ttttcatgat caaggtgatt cattactata accatcggct tttctgcttt ctcaacataa
     4261 cgaccatgta tttctgttcc tacttctata ccatttgcag tgttaataag cattacggca
     4321 atgtcggcag ggaataatga agacactaag cctccaacaa agtcgtccat accaggatta
     4381 tctaaaatgt tgatctttgt attgtttatt tcagtgtaca ttactgttga atacaatgag
     4441 ttgccatttt cttgctcaat taaacgataa tctgaaactg tattcttgct agcgatgtct
     4501 cctttacggt taataacgcc accaatttgc aacatagatt ctgctaaaat agtttttcca
     4561 gaaccagagt tgccaactaa ggcaatattt ctaatctgtt cagtttgata atttttcatg
     4621 ttaatactta aatattaaat ttgtataatt tttacttttt tatttggctg acaaaaataa
     4681 taaaaataat atacttacaa agtatatcag aacattaatt tataaaaaaa cttaaattta
     4741 tacttaaaat aaagtttgaa acttaatatt gttaattaaa tttgcaacct aattttaaat
     4801 aatgaggaaa aaattcatta taaacttagc tttccttctt ttattaaatc ttttaataaa
     4861 accgttttgg atttttggag tagaacgctc ggtgcaaaat attgtaggct ctgcagaatt
     4921 tggattttat ttttcattgt ttaatttttc tcttttgtta aatattattc ttgacttagg
     4981 aattacaaat tttaacaata gaaacatttc acaaaatcca caactattaa gaaaacatgt
     5041 gtcgaatata gtttcgctaa aactaatatt ggcattggtt tatgctgcac taaccttagg
     5101 agctgcttta attataggtt acgactcaag acaattagga atattgttaa tattaatttt
     5161 aaaccaattt ttatcttctc taattttata tttaagatct aatattagcg ggttacaatt
     5221 atttaaaacc gacagtttaa tttctgtact cgacaggttt ataatgatag tagtgtgtag
     5281 cattattatt tggggaaatg ttaccgacaa acctatgaaa atagaatggt ttgtattaac
     5341 tcaaacattt gcttatttaa caactgctat agttacattt tcaatagttg tttataaagc
     5401 caaatttttt aaactaaatt ttgattttag attttttaga gtttttttac gacaaagcta
     5461 tccatatgcc ttattgattt tactaatggc tttttacaat agaatagatt cggtaatgtt
     5521 ggaaagactt ttgccagacg gtaaacacca agcagggatt tatgctcaag cttatagaat
     5581 attagaagca gcatcaatgt tgccatattt attctctata ctgcttttgc caatgttttc
     5641 acgaatgatt aagaaaaaag aaaacattac tgaattagca aaattatctt tttcaatgat
     5701 tttagttcct gctatatttt tagtgtgtgt ttgttacttt tttagcgagc aaataatgga
     5761 tttaatgtat tgggaaaacg ttgaggcaag ctcgaaaact ttaagttttt taatgatagg
     5821 ttttatagga ataagtacta cttatatttt tggaacattg ctaactgcaa acggaagcat
     5881 gaaacaatta aatacaatgg cttttgttgg aatgatttta aatatagctc ttaatcttat
     5941 ccttattcct cgtttacatg taataggctc tgctatagca ggaatgataa cacaattgtt
     6001 tactgggttt tctcagctta ttttggcaaa atatgtattc aaatttaaaa ttaattacaa
     6061 actaatctta tctatagtaa tttttgttgc tattgtaata gtgtcaactc aacttttgag
     6121 caaattgaat atgaattgga taacaataat agtaattatt ggattgattt ctttagctac
     6181 atcgtttgct acaagactgt ttaagataaa agatatttac aaattattaa cagaaagaga
     6241 gtaaacatat ttctattttt ttgttataaa taaaaaatta gtacaactta ttcttgaaaa
     6301 aaactaaatc ctaaaaaaaa tacatatctt tgcatttagc taaagtaaat caagctgtaa
     6361 aatggtaaaa aataatatag aattatcaat agttcttcct gtttataatg aggaaaagaa
     6421 tatcgattta ctttacacaa aattaatttc aagtgttgag caattaaaat gtgcttacga
     6481 aattattttt gtaaatgatg gaagtattga taactcattg gagataataa taaatttagc
     6541 taaaaataat ccgaatatca aatacattga ttttagcaga aattttggac atcaaatcgc
     6601 aatttatgct ggaatggaaa attcaaaagg aaattatgtg cttacaatgg attctgattt
     6661 gcaagaccca cccgaagaaa taataaatct ttataacaaa gtaaaacaag gatttgatat
     6721 agttcatgca agacgcaaac aaaggaaagg agaaactata tttaaaaaat taactgctaa
     6781 aatttattat cgaatattga gaaaaattac taaatgcgac atacctgttg atgtgggaga
     6841 ctttagaata atttctaaaa aagttgttga tattttaaaa caaatgcctg aacaagaaaa
     6901 atatttgcgt ggacaaattg cttgggtagg atttaaacaa acttttatag aatacgatag
     6961 agaccaaaga gcagaaggca aaactaaata ttcaacatca aaaatgacaa aacttgctct
     7021 tgatggaata acttcttttt cagactttcc tttaaggttt gcaacttatt taggtttttt
     7081 cgttagtata atatcttttg tgcttatgct ttgggtgttg tataaacgac ttattgtaaa
     7141 agaatatgtg cagggatgga cttcaataat tttgagtgtt ttgtttatgg gaggaataac
     7201 gctaataaca attggaatta ttggcgaata cattagtaga atgggcaata atgtgagaaa
     7261 acgccctttg tacatcatta ataaaacgaa tttagataat gaggaagaag ataaagaaga
     7321 agcaaattaa aatattatca attgttttat tacttgttgc tatcccttgt ataatttgca
     7381 tacttggtaa taagtttgaa aacaaattca aaaataaaga aattgttgtt agtgctagca
     7441 ttaaacctat aagttttttt ttaagttttg aaaataataa ttacttaaat gaaactcaca
     7501 aaataacaaa cttaaaattt ttatccggac gaaaatctgt tattatcgac aagaacggcg
     7561 ggcaaagtcc cattattaca attccaattc ccgaccacgc agatatgagt tttgttaata
     7621 tcaactgtaa tttttggctt tcaccagata caagtttcat aaattcatta tttgtgttta
     7681 caattataga cactaacaat actgtattat accaacaaga aagagagata aaggcagaaa
     7741 acttaaattc aattaattgg tataacttta acgaaaattt caatattcaa acacaatact
     7801 taaactcaca acactttttg caaatgttta tacgcaataa tttagagcat agttctaatt
     7861 tgtattttga tgatttagaa attgtgtttg aaaaagaaaa tcataaaact aaagcacttt
     7921 tagttgattt tgaagatgat aaatttcaaa aaactagctc aaaatactgt ttttcaggaa
     7981 attttagtac ctatgcaagt ggcacaaacg attactcaga acaagttgta ttagattttg
     8041 aaaatattaa ctacaaaaat ttagataaaa tattacttaa cctaaattat ttgagcgaaa
     8101 cagaagactt agatgcagtt tttattgttt ctatttgcga tacaagcgat actgaaattt
     8161 tttgtagtga attagaactt cataaagata aaaatttcag acctgaaaaa tgggaaaaca
     8221 ttagtactga gattattatg ccaaaaagtg ttgttgataa aggtagtttt ataaaatttt
     8281 atttgtggaa taaaaattct aataccgtat ttgttgatga tgtgtattta atatttaaag
     8341 aattttcgat tgagtatgga aataaaccga ctgctcataa ttttatttta aatcctaatt
     8401 ttcaaataaa aaaaaatcaa gcaccataca acttttatta cctaaatcga gaaaagaaat
     8461 tcgctaataa agaaaaagaa ctaagtaagt tttttactga ttttaataaa attctaattg
     8521 gaaattttaa agacaataca aatttagatc aaatacttgc tattggcact gtcgatatta
     8581 tgttttatga ttttgaaaat caaacaaaaa ttaaacatga ttttgaaaat ttaaacaact
     8641 tgcaattttt tacagatggg caaaatgttt tttcatatca agaaaaagca aaaaagttag
     8701 gtttacataa aattaatttt tcaacaaata gcttagactt ggaaacagaa atagtgtata
     8761 attccgaaaa taaaatttct gaaatatttt caatcaatga tagtataatt tcaatatttg
     8821 ataattttgg aaatgtgtat gattatttaa aaaaagatgg aaaatattct ttgataaaaa
     8881 catcaaaatt aatttcatgc actaaaggta atttaaaact attaaaatca aatttttata
     8941 atgaagaaac agaagaagtt ctaattattt ttcaagagac agagaaagta aagtatgagt
     9001 tttttactta ttgtataaac gaaaatatgt ggaaacattc aacaaattgc tctaatacgt
     9061 ctatacaata ttttgataaa ttagacttgc aatctgaata ttttttaatt gactataatg
     9121 gagatggtgt ttttgaactt ttgaaattta acagaacaaa taaatttaac ttaaaattac
     9181 ttagttttga ttttagaagt tacaaaatac tttatgaaat tgattttaaa ggatttgaaa
     9241 atagtcaaaa tccaaaatat tacgaaaaac taaaaatttt aacaggaaat ttcgcagata
     9301 ttcgaggttc tgaagtgata atttttcaag acaatgtaaa taaagtagat tggttaaatc
     9361 aaaaaacaga aatatattca ttcaacaaat aaaatgaaaa tttataaaat ttatatatta
     9421 tttttaatta cgctattatt tgtttcttgt ggctctgata agaaaaaaac ttgcgattat
     9481 agtgtttcag atattgattt tttagcaaat tatgattcta acgaattaga ccttgattat
     9541 gaaaatgcgt taagtggttt tacaattccc tcaataaatc aaaccgaaag tggatatttt
     9601 aattttaatt ttaaacttac aaataattca aacaaagaaa aagaatactt ttataaaatt
     9661 tattatcaaa acgaatcgta taaaattcaa gaatgtgatt ctaaaaataa agctcagcaa
     9721 gctcaatatg tagaggaaaa tttttatggc tcttgggaag atacaaatat tggatttaaa
     9781 agtttaggaa aaattagccc ttcagagttt attgttctta cagactcttt ccggattgtg
     9841 ggtaatccta gaaatgaaga aaaatatttt ttctatggcg aaaacgacag atggaaacgt
     9901 aatccacgag tgggcgaata tagttttatg ttagtgattg ttgatgagga aaattttgag
     9961 caaattcctg aatacataaa gcatataaat ttgttaaaag atagtgcaaa cttcgtgtcg
    10021 ccctattatt atttccttta tggcgaaggc aaagacataa aaaattgttt cgtaaaatta
    10081 gaagagaaag ctttaaaagt tactgctaaa cccaatttag gtggtggaat ttatgtaaat
    10141 cattacatgt ttgatgaaac tatagaagat ttagataaat accaatcttg taattgcaac
    10201 aatagttcaa tggaagaaaa agcacatttt gaacagtttt tacattatat agacgaatct
    10261 tctagactgc ataatgtacc agtaattaaa gatgttctga atgaaggttt tacaaatgaa
    10321 gaatacaact ggctttctgc tttttataaa attgaagata gaattactgt tctccctcaa
    10381 ttagcaaaat atccttgtca aacaattaaa agcgattctg ttaacaataa aattataatc
    10441 tgtaatcctg catcaacata tggtaactgg caaaaacaaa atgtaggagt aattgctcga
    10501 catgggtttg tgtatggaaa atatacagta aaagctaaac ttacaagttt gttaaatgat
    10561 gacaatgttt ggaacggtat tacaaatgca atatggttaa taaatcaaaa aggacgaggc
    10621 aatgaaaccg gctggaattt aagacgttct tgcaataaaa atggatatat gaaaacttat
    10681 tggggtggaa gaaatgacga aagagttgaa aaagttgatt attcagaaat tgattttgaa
    10741 atattaaaaa caacaccata ttgtcccaga acaaaaacaa aattgacaaa atctgaccaa
    10801 gctatgccag ataaaataga ttcatggaat atagaactgc ccgaaaatat taaaaaagac
    10861 gaagggaaaa tagctgtttg ttgtacaaac tgggatatgg cttgctggga accaaaaaac
    10921 ttttcaacag gttgccataa aataaaatat aataacaaaa cattttattt acatagatgg
    10981 gataatacat atagagcaat aacgttaaaa tatctagcct caaataaaga actttttgga
    11041 ggagattatt attattatca aattgattgg caaccagata aaattatttg gagaatagga
    11101 ccagaaaaag ataaacttta cgaagttgga tatgttgacc aaactgttac tatggtgcca
    11161 aataatcaaa tgacactaat aattactcaa gaatttcata atacgaattg gtggcccggt
    11221 tcaccttacg gacaagaaaa tattccattc ccagcaaaag attatgtcgg agaaatttat
    11281 gaaattacaa ttgagtaaat gctaataaat agaaattttt aacgatttct ataaaaaagt
    11341 aaaaaaaaat atatattaaa acaactattt ttgttttttt tcgtcaatag tcaataataa
    11401 tgaaaatatt aaaatgtttt tataacttta cacaattatt aaagtttatt aatagtataa
    11461 aatttttaaa atgaaatagg agagataaaa atgaaaactc tttttatttt tttacttatg
    11521 tttgtcgcaa atatagctgt ttcacaagtg gtttcagata cattagttaa aaatttaggt
    11581 aaagttaatt cagactttgt ttatgatgag gacaatttag ttttaatccc caatactaat
    11641 atcaagatgc aagcaccaga gtattttttg ataagtgaaa atattccagg acttgtacac
    11701 ccaggctcgt cagctacagt gcaagtacaa gaaatcacag gaacatctta tattatgata
    11761 agccaagcaa tgacagataa acatttcgaa agtcaaggcg taagacttat atctacaaac
    11821 gaggttacaa tgcaagacgg taaaggcgga attttatatc ttgttgaatt tgaggcaagt
    11881 ggtataattt atgaaagact tatgcttttt gctggagatt ataataatac aatttggata
    11941 aatgcaaatt atcctaaatc tgcaaaaaaa ttacttcaag atattttaac tcaaagttta
    12001 ttaacagctc aatatactaa gcaataacta tggtaagaaa atttttaatc atattagcaa
    12061 tatttgtatt ttgcgggtgt ggattaacaa aagcccaaaa tatccttgtc cacgaaatac
    12121 catatagcac ccatcatcaa gatatgtggg gcgttggaga agcttttagt ttagattttg
    12181 attatgactt gtttaatatt gcagtacaag atggttttac tgtcggagaa attacagaag
    12241 tgctaggaca gcaattcggt gtgatggttg atatgggctt ttatatattt ttacgttcta
    12301 cttttagtat tcatggattt actttaggat cggtcgatgt tgactaccct gtaagaatta
    12361 cctgggattt tccagatgat tatacttttg accatggcga aactattact ataaattctt
    12421 cttatgacgt gttagacggc tgggctttag atactcactt cccaacagca ggagtaatag
    12481 cactagacct tgaatatgga tttggtttgc atctcgatat tatcgtttgt ttgtttagtt
    12541 gcgaaacaat tcctattatt ccaacaataa acattcctgt agtaccatat tctaccgacc
    12601 ccttaccaca tgactctata gcaatttttt atctgaatgg tcaaacagga gaagtagcat
    12661 acccatgctt agaccctgtt acaggattgc ctaatatttg taacgatgat attttgccta
    12721 ttgttattcc agattggttc ggcataggat taaccggcga aattgatatt ccttatgtag
    12781 taacagaaga ttcgtataat cctaatactc aatgcttgca tgctcatgga agcgatgaat
    12841 ggttatggtt caatttagac attttagaat ttctttcatt tattgctggc tttataccac
    12901 cacctaatgg accagcaatt caacaagcta ttggaattat aaacggacaa actatagaat
    12961 atgaaataat tgctggagtt acagctacta ttgaatattt tattttacac ttagatttac
    13021 acatgtcaag tcatatgaca caagactttg ctttttgtcc aactatatta gcaactctta
    13081 gattcccaat cgacttacca tatagcgaaa cagatccctc taatggaaac gctgttgtag
    13141 ctgaaggtgt aaacgatact atcactatga tagtaaataa tgatttacac attacttatc
    13201 cttgttatga atgggattct atgcaagttt atgatattac ttacaacata attagtactt
    13261 tttctaacca tacatgggac tctattgctt ttgactttat cttagaggca attttctttc
    13321 aaatatcaat accaactagc tttttacctg tagcaaaaat gccagagttc tgcttgcctc
    13381 aaattcaagc aaataatgca gacttaggaa cttatcaata tattaatgct tgtgctcctg
    13441 caataacagc accagcagct tctattagta gcgtttttga tgaaagtaca gaaactaaag
    13501 gaggtagtgt ttcgcaaacc aaagacttaa ctttctgttt tccaacaaat tgtgagcctt
    13561 tgatatctga atccttccca ttgggtagta ttcctttaac ttggtttgac caaacttggc
    13621 tactcgaagg ttttgtacaa gatactgttt tccctggcac atggcttttc cctttgcctg
    13681 aacttgatat tgaaattacg gcacaagatg ttatttgtta tggtgattct acaggagtaa
    13741 ttacagtaga agctctacat agtacaggac cttatacttt tgagtattca tggggaaatg
    13801 taaatacaca cttagatcct atagatgaga taaatgtgcc atcaggatat tattatgtaa
    13861 cattaacaga tgtttatggc tgtcaagttg aaggcgaaat aaatgttgct gatgaaaatc
    13921 cacctatctt atcaagccta tatgccgatg atgtgttgtg ccatggtgaa gcgacaggag
    13981 ttttgtattc tcacgtttca ggaggagtgc caccatacac atggaattgg caacctagcg
    14041 gaagaacaga acaaaatcca tctggagttt ttgcaggatg gcattattta acaataaccg
    14101 atgcagtagg gtgcgaacac tacgactctg tgtttgttga cgaaccagat gctcctctaa
    14161 ctatggatta tattagcgga aacgtttctt gttatggtta ctatgacgga tatattgatg
    14221 taacaccaga aggcggaact ccaccatact actatcaatg gtcaaatggc caaatgacac
    14281 aagatttaat caatatccct gcaggacaat atattactac aataactgat gcaaatggct
    14341 gtacaattac cgaaactttt gatattacac aaccagaccc attagaatta actacatata
    14401 ctcaagatgt gttatgttac ggtgaaaata caggaagcgt tgacttggtt gttactggtg
    14461 gaacaccacc atatagctat acttggagta ataattcaaa taatgaagat ttattagatt
    14521 tgttctatgg aatttatatt gttactgtag tagatgcaaa taattgtaca gaatatacta
    14581 tggttcaaat acaacaacct gctttaccac tacatggaga tattacgcca actcacgtaa
    14641 gatgctttgg cgaaggaaat ggagttgccg atctaaatgt atttggcgga acaccaccat
    14701 attatttctc atggagtaca ggtgctatta gcgaagatat agaaaatcta attccaggaa
    14761 tttattctgt tacaataaat gatgaacatg gttgcgaaac agctgatact gtacaaattt
    14821 tccaaccaga tgctccaatt gttggtacaa tttcaggaaa tgatattact tgcaacggag
    14881 gaaatgatgg taatatttat attacagtac aaggcggtcg tccaccatat acttttgaat
    14941 ggtcaaatgg aagttgggag caaaatttaa taaatgttca agcaggaagc tatacagtta
    15001 ctgttacaga ccaaagccat tgccattatg aaatgaatat agaacttact caaccagaac
    15061 cattttatgc tcaaataatg gatgacttaa caatttgtta tggtgaatca gttgaaatcg
    15121 gaataggaat aattgaaggc ggaacaccac cttatacaat agtttggagc aatggagacc
    15181 aaggaatgag tacaactgta agcccaacag aaacaacaac atatactgtt cagattgttg
    15241 atgcaatatt ctgcgaaagt caaactgcgg aaattacagt taatgtacat aaaccattaa
    15301 gtttagaagt gagtgcaagt gctactaaag tttgtcctgg
//
LOCUS       DAOPNX010000065        15332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_4597_length_15332_cov_6.242194, whole genome shotgun sequence.
ACCESSION   DAOPNX010000065 DAOPNX010000000
VERSION     DAOPNX010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15332
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4597_length_15332_cov_6.242194"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..794
                     /locus_tag="PKN53_07825"
     CDS             84..794
                     /locus_tag="PKN53_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOD88893.1"
                     /translation="MTDYYKLLLQDVRFKEGLTACMNCGVCTAICPAAEFYKYDPRQL
                     CSIVQTRDNEKIEELLKSETIWYCGQCMSCKARCPRGNAPALVISVLRKLSQELGFFV
                     ESEKGQQQFALKRTVGDNILNIGYCVHVDTVHPKLHPEQGPVWKWYFENAEELADRMG
                     GNYNKYGPGALRIIGEKNLDEVRKIFEVTGGKKLNDHIEEFSKQKAKSTNTSFENEGI
                     NNEYFFQTYIKNSENHQK"
     gene            800..1873
                     /locus_tag="PKN53_07830"
     CDS             800..1873
                     /locus_tag="PKN53_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOD88894.1"
                     /translation="MTKTKHYWNNYQKEIAEDNYFYERSCIRQSFFPGSETTFLKILR
                     EELGKTIIDDPNQHTCTGIGYHSDVVPFDTTMTICARLFSLMKDAGLKNYVPSCVTSF
                     GVFTEVIETWQNSPEELEKTRNFLRQATGREFDIPENIVHPSDIIYKYRKELKSKMKY
                     QLVNHKTGEPLKAVEHIGCHYGKIFSKHAIGGAEFPQVLAGMIEEWGGEVIDYPERRH
                     CCGFGFRQYLVMANRGYSVANTKKKFESMQAYKPDFILTNCPGCTMFMDRWQYTIAEM
                     EGITYDDEHMGIPVLTYEELAGLLLGYNPWDLGLQLHQVQAESLLNKIGIEYDPKDKF
                     VGKSGTFLGEPEKPKNLIVDYEK"
     gene            1863..2888
                     /locus_tag="PKN53_07835"
     CDS             1863..2888
                     /locus_tag="PKN53_07835"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD88895.1"
                     /translation="MKNKSVIVIGAGVAGIEAASNIADQGYSVTLIEKKDVLGGNLTN
                     YAKLFPNFADADEVKNNLINNLSTKKVEILKNNDITKLFFENDKWHAVNSQNQTYSAD
                     VALLTTGFTVFDAKRKEELGYGIYDNVITSLEFESMYKTGKITTKQGNIPKRIAFLNC
                     VGSRDEKVGNHYCSRVCCINAVKQATEFKTLYPESEAYCFYMDIRMAGQFYEELYRKS
                     QEENNVIYIRGRISEAAATIDNRIQIKTEDTLTGLPLKMTVDLLVLMVGMEASEATSN
                     LSKQNEINGEYGFAKSLGLHGNDNKTTKKGLFLAGSCKRPLNIPEAIADARAAALQIT
                     QYLNNLQ"
     gene            2885..3232
                     /locus_tag="PKN53_07840"
     CDS             2885..3232
                     /locus_tag="PKN53_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOD88896.1"
                     /translation="MKNWGFKIAKPRIIDLDTANTKAFEELCEKVPSAKRCIMCGACT
                     ATCSAANHTSFNFRKCNIMFRRGQFEGLAEELDKCMLCGKCKLVCPRAVNTRAFIYNM
                     RIFLNDLNYKNIK"
     gene            3232..5058
                     /locus_tag="PKN53_07845"
     CDS             3232..5058
                     /locus_tag="PKN53_07845"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOD88897.1"
                     /translation="MNYSHFILPFSIGAIILFVIIIVKFTKWFRLLDRKQKYFIKRNI
                     FTTKTISATIEAFREALIHRNIYKKNPLLGYMHMSLAFGWFLLIVVGKIEASVYSGTF
                     FEEPWLAIFFKYFAREPHNFWGAKFFTFTMDFLLLIILSGLALAIIKRFRSKILGLKK
                     ATKHIIYDKIALSALWLIFPLRLLAESSTAALVQNGGFLTGGLGSLFPQQFAQAIELP
                     LWWAYSISLFVFFVSLPFSRYMHIPTEVALIFLRKWGATEGEQYSGLTNFEINSCSRC
                     GICIDACQLNFSANINNVQSVYFIRDTRYNKISDLVANNCLLCGRCTEACPVGLELTQ
                     IRQQLRTKTEVPGKHYYDYCKVEPTKNKTDVIYFAGCMTHLTPSIILAMKNIFSAANE
                     KVWFMDEEKGICCGRPLRQQGFVQQSKDLVSKNTKLISSSGAKLLVSSCPICCKSFKE
                     EYALDIEIMHHSEYIKMLIDEKRIKLNKSELNIVFHDPCELGRGLNIYKQPRQVLSKV
                     GKLVNTEFDEKDSLCCGGSLANTIIELDSQLKIRDDVLQILAKDNPDVLATACPLCKK
                     TFTRNYKGKVMDIAEIVSNFLSLQNNSKKEGKKEEEKILISQ"
     gene            5080..6342
                     /locus_tag="PKN53_07850"
     CDS             5080..6342
                     /locus_tag="PKN53_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOD88898.1"
                     /translation="MVDIFDRLKEARGPLGQYQQKAEGYFMFPQLEGEIKPRMKFLGK
                     EVLTWSLNNYLGLANHPEVRKADADAAKEFGMAYPMGARMMSGETKYHKELENRLAKF
                     VKRESAYLLNYGYQGMVSIIDTLATRKDVIVYDSEAHACIMDGIFLHKAKGGKSYVYP
                     HNDIERCEKMLKFATKTAKENGGGILVITEGVFGMAGDLGNLPEIVKLKEKYNFRILV
                     DDAHGFGTMGETGAGTDEHFGLQKDVDIYFATFAKAMAGIGAFVASDEVIVDALKYNM
                     RSQIFAKSLPMPMVIGALKRLDMLQNQPEHKEKLWKIAKALQKGLKDNGFNIGNTQSP
                     VTPVYFSGSVPEGTNIVVDLRENHNIFCSMVVYPVIPKDLLMLRIIPTAAHTLEDVNE
                     TIEVFKKIKANLEAGVYNTKEIQSMKVE"
     gene            6407..7720
                     /locus_tag="PKN53_07855"
     CDS             6407..7720
                     /locus_tag="PKN53_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOD88899.1"
                     /translation="MKESKYYLQCVKCEHITEDFGAWFEQNQVCPKCGSKHSEVWYNS
                     DYNELPLIMKGNPESFWQYFYYLPLNRRENIITRNEGAISLQKWWFLEDFAKEKYGLN
                     LDIYVYRNDLNGGTGTFKDVAAALAASVLKEYDINQYCVASTGNTATSYARYLSIAGI
                     NCSIFLPENALKSSEAMISAFGQNVYRVKGDYSKAKEIAAEYAKKYNILISSGNIDPL
                     RVEAKKTMVFEWLRQIGKMPDVYIQAISGGTGPIALDKGVREIKKTFPEIKNPRMIMV
                     QPDACDPMTQAWEKAEAKNFPKNYENDYPILDNPQTQVPTLATGNPATYPIVSRIIKK
                     SGGTFIRMKEEKLVPIGRLVAYERKLLLGPASAVCLGGFFEALEKNLLKSDETIIINI
                     GEGVRRAPEFVEQMIYTSKDISSVDECKPHQIDDYRQQLWNDVLK"
     gene            7867..8820
                     /locus_tag="PKN53_07860"
     CDS             7867..8820
                     /locus_tag="PKN53_07860"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOD88900.1"
                     /translation="MSKRIIVTGGAGFIGSNLCDYLLNQENEIVCIDNFDEYYPPKQK
                     QNNIKQALKNPNFTFINEDIKNKNKLSKIIDGEFDAVIHLAAKAGVRYSIKNPIGYEE
                     INVQGTKSILELAIQKNIEQFIFASSSSIYGNAPTPFDENFTDLKPINPYAQTKLLSE
                     QIGKEYSEKYNLRFISLRFFSVYGPRLRPDLVMNKIANSIYKNEKLRIFGDGSTARDY
                     TYVDDIVLGIDKALNYKNTKFEVFNLGNGKPVKLSEIIQIFEKETNKKLKINNIEPIN
                     GESEITWANNKKASELLGFKCQTDIEHGIKNYLTWYKNHCL"
     gene            8817..9632
                     /locus_tag="PKN53_07865"
     CDS             8817..9632
                     /locus_tag="PKN53_07865"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD88901.1"
                     /translation="MKKTIEANSKMSIFSYLKLIWDYRFFSHSLAIGEIKNKYAKTYT
                     GIFLSLIQSLIGLAVYWLIFGIAIKIDTGNIPYPVFALPGIIIWQFFSYLVGTSSGAL
                     ISSEHLIQKLYFPRINLTLAKVLPGLVDLAAGFILFVILFFIFGLTPNFGWFYIFPII
                     LVLIISGLAVGLWTSILSLYIRDLSQIIIQITNFVIFITPVFYPGTIVPENFRFILYI
                     NPVAGIIEYSRAALFCTAIPDNGYLIGFAIVFILFISAIFIYKNLEKKITDLL"
     gene            9629..10876
                     /locus_tag="PKN53_07870"
     CDS             9629..10876
                     /locus_tag="PKN53_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD88902.1"
                     /translation="MIQHEKAIVIKDLNKKYYKKKQSSSNLFKNIKSNNNTESFFALK
                     NINLEINKGEIIGIIGPNGAGKSTLLKILAEVTPPTSGKIEINGKIASILEIGIGFQP
                     DLSGYENIFLSGRLYGLSKKQIKSKLEQIIEMFGFADFINTPVKYYSSGMYMRLAFAI
                     ITNIEADIYLFDEILSVGDLGFQSKVLSEIERLKSTGSTILIVTHSPKNIYQICDKMI
                     LIHLGDVIFTGSPNETILAYRNIIYSSQNVRQSRKSNVLNTDILKKFKSSDIKPEIAD
                     FNLIFFSISNSPNEEGETLRCDKDIFLKLIFNYHSPQDFKLILVLQDLQGNAINTHFI
                     NINLSHSIIEKEIVLLIEKNTFNEMSIMFDILISSLDDKILINYRNLCETTLESNNNL
                     SKLMGYINIPIKEIKKTRNANTI"
     gene            10860..11957
                     /locus_tag="PKN53_07875"
     CDS             10860..11957
                     /locus_tag="PKN53_07875"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOD88903.1"
                     /translation="MQIPFNKFNSFYLKNKEKTLSKLDKVLNSGKYIRDKEIDFLEAN
                     IKKLTKRKFTITTSSCTDAIFLALKTIDIKPGDEIILPSFSYIASLSPILMCNAKPVF
                     VDISPETLTLDFDLIKKAITPKTKAVIFVQLFGYSQDLSELKSFLKGKNITLIEDAAQ
                     ALGSKVNNLNGGEQGDLSCISFDPTKIVSAFGTGGIVLTDNQEYYDKLTKLIHHGRNQ
                     NGEFEILGYNSKISELNASIINLQLENLDSILERNNNIANQYFAELSNNKNIKILKPN
                     SNQFSTYHKFVIQTRDRNELRKHLNNNGIETKIHYSTLLHEQKLLKSFEFKTHNLQNS
                     EYAKEKVLSLPIYPNLTKEEISYICNLINNY"
     gene            11962..12549
                     /locus_tag="PKN53_07880"
     CDS             11962..12549
                     /locus_tag="PKN53_07880"
                     /inference="COORDINATES: protein motif:HMM:NF036822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88904.1"
                     /translation="MILVGCGSAGKETLAMLLEQGKTDNIFFYDENKNAEDFIFEKFK
                     VIKKTSKLLDNLKIDNKFCVCIGNPRRRKKMYNKLIELGGSPNNVIWDKNLSISELSQ
                     NATIIQPGVHISYEVIIGKSCLIHNNTSIGHKTIIGDFVNIGPLCSLIGPCTIGNETY
                     ISAGSIILPNVTIGNNVYVKAGSIVNRDLKDFETF"
     gene            12561..13886
                     /locus_tag="PKN53_07885"
     CDS             12561..13886
                     /locus_tag="PKN53_07885"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD88905.1"
                     /translation="MLNKHHIKEYNTSLNRKGLKRICYAPYTSLRFSQSGNIISCCYN
                     RGYILGKFPENTIKEVWNSKKLKALQKNIKKNNLNLGCEICKDRILGKTFTLTGSFHY
                     DDLHYYYKSKYPAMLDFEISNTCNLECIMCTGENSSSIRANRENLTCLSPIYDSSFVE
                     QLKEFITHIKEARFSGGEPFLNNLYYDIWELIIKLNPKAKITVSTNSTILNKKIKDLL
                     DKGNFTIAVSIDSFTKSTYEKIRQNASFDSVVENMKYFYEYSIKHNKPFFINLCPMPY
                     NWKEIPEIIKICNSKSIKLIFHTIVFPPKESLWALDSETLAEISNFYDDFEFIANSEI
                     EFFNYSNFKNLKKQIKTWHSEAIEREKKINLLSSFSYEELLLAFQKHLGENNYDFYQQ
                     IMQLINDFDIKKRERIINKLFFFHKEALFSELIHNNTERLLIKLSMFDY"
     gene            13893..15308
                     /locus_tag="PKN53_07890"
     CDS             13893..15308
                     /locus_tag="PKN53_07890"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD88906.1"
                     /translation="MFKKIFTKNKIWNPLNKKIIKDFNKTREISKRKYLCHAPFKSLC
                     FSHSGDILACWYNKMYPLGKYPNDKIKDIWFSERAKKLREHILHNDLNFGCYDCKRNI
                     ENKFYHAQSPYRYDFLPDTTSKYPVSMDFQISEQCNLKCIMCNGEYSTRIRQERENKK
                     MIQSPYDDVFYEQLKEFIPHLKEASFSGGEPFVGSKFFKIWDLMIELNTNIKLSITTN
                     GNVLNDKVKYYLNKLSFHITLSLDTINPEIYKNIRIGGDLNIFLHNFDWFYEYAKNKS
                     TYFNVKTCVMQQNWHDLPDLAEFLNKRNVPFLYNNVFYPPNASIWNLSSTKLSEIKTY
                     LENNFVFHNQSIVQSYNAETMKGLLRQIDSWQKQALYSESFELNKMTTKELEKFLLNN
                     FKTFILNEQKENINSYPFSYLEIEKIITKLVQDTKNEYLVNNGLIYFCKSPVEKIISE
                     FRIRNYETIKDRFIQACKFTC"
BASE COUNT         5940 a         2155 c         2391 g         4846 t
ORIGIN      
        1 agggtatttt taccaacaaa aaacaagtat ttttagtcta tttattgttt tttgcactta
       61 tttattttgc aaaaaatatt aaaatgacag attactacaa gttacttttg caagatgtta
      121 ggttcaaaga aggtcttacc gcctgcatga attgtggagt ttgcacagct atttgtccag
      181 ctgccgaatt ttacaaatat gaccctcgcc aactttgtag catagttcaa actcgcgata
      241 atgaaaaaat cgaagaactt ttaaaaagcg aaacaatttg gtattgtggt caatgtatgt
      301 cgtgcaaagc aagatgtcca cgaggaaatg cacctgcttt agtaatttct gtacttagaa
      361 aactttctca agaattaggt ttttttgttg aaagcgaaaa aggacaacaa caatttgctt
      421 taaaaaggac tgttggagac aatattttaa atattggcta ttgcgttcat gttgacaccg
      481 tgcatccaaa acttcacccc gaacaaggtc ctgtttggaa atggtatttt gaaaacgccg
      541 aagaacttgc cgacagaatg ggaggaaatt ataataaata tggtcctgga gcattgagaa
      601 ttattggcga aaaaaattta gacgaagtaa gaaaaatatt tgaagttact ggaggcaaaa
      661 aattgaatga tcatatagaa gagttctcaa agcaaaaagc aaaatcaaca aacacctcat
      721 tcgaaaacga aggaataaat aatgaatact tttttcagac ttatattaaa aattctgaaa
      781 atcatcaaaa ataaagcaaa tgacaaaaac caaacattat tggaataatt atcaaaaaga
      841 aattgctgaa gacaattact tctatgaaag aagttgtatt cgtcaatctt ttttccctgg
      901 ttctgaaact acttttctaa aaattttaag agaagaactt ggcaaaacaa ttattgacga
      961 cccaaatcaa catacttgca ctggcattgg ctaccattcc gatgttgttc catttgatac
     1021 aactatgaca atttgtgctc gccttttttc tttaatgaaa gatgctggct taaaaaacta
     1081 tgttccgtct tgtgttactt catttggcgt ttttaccgaa gttattgaaa catggcaaaa
     1141 ttcaccagaa gaacttgaaa aaactaggaa ttttttaaga caagctacag gcagagagtt
     1201 cgacattcct gaaaacatag tccacccttc tgatattatt tacaaataca gaaaagagtt
     1261 aaaaagtaaa atgaaatatc agttagttaa tcacaaaact ggcgaaccac taaaggctgt
     1321 cgaacatatt ggttgtcatt atggtaaaat ttttagtaaa catgcaattg gtggtgctga
     1381 atttcctcaa gttttagcag gaatgataga agaatgggga ggcgaagtaa ttgactatcc
     1441 cgaaagaaga cattgttgcg gatttggatt tagacaatac ttagttatgg caaacagagg
     1501 ctattcagtt gcaaatacaa aaaagaaatt cgagtctatg caagcttata aacctgattt
     1561 cattctaact aattgtccag gttgcacaat gtttatggac cgctggcaat acactattgc
     1621 cgaaatggaa ggcataactt atgatgacga acacatggga attcctgtac taacttatga
     1681 agaacttgca ggacttcttt taggctataa tccttgggat ttaggattgc aattacacca
     1741 agtgcaagct gaaagccttt taaataaaat cggaattgaa tacgacccta aagataaatt
     1801 tgttggtaaa tccggcactt tcttaggaga accagaaaaa cctaaaaacc ttattgttga
     1861 ttatgaaaaa taaatctgtt attgtaatag gcgcaggtgt tgcaggaatt gaagcagcaa
     1921 gcaatatcgc agatcaagga tatagtgtaa cattaattga aaaaaaagat gttctaggcg
     1981 gaaatttaac taattatgcc aaattattcc ccaactttgc tgatgcagat gaagttaaaa
     2041 acaatttaat caataattta agcacaaaaa aagttgagat tctcaaaaat aatgacatca
     2101 caaaattgtt tttcgaaaat gataaatggc atgcagtaaa ttcacaaaat caaacttatt
     2161 cggctgatgt agcactactt acaacaggct tcactgtttt tgatgcaaaa cgaaaagaag
     2221 aattaggata tggcatttat gataatgtaa taacttcttt agaatttgaa agcatgtaca
     2281 aaactggaaa aattaccact aagcaaggta atattcctaa acgaattgct tttttaaatt
     2341 gcgttggaag tagagatgaa aaagttggaa atcactattg ttcaagagtt tgctgtataa
     2401 atgctgttaa gcaagcaaca gagtttaaaa cactctaccc agaatctgaa gcttattgtt
     2461 tttatatgga cataagaatg gctggacagt tttacgaaga attatatcgc aaatctcaag
     2521 aagaaaacaa cgtgatttac ataagaggaa gaatttctga agcagctgca accattgaca
     2581 atagaataca aataaaaact gaagacactc taactggatt gcctttaaaa atgactgttg
     2641 acttacttgt tttaatggtt ggaatggaag cctctgaagc aactagtaat ttaagcaagc
     2701 aaaacgaaat aaatggcgaa tatggttttg caaaaagttt aggattacat ggcaacgata
     2761 ataaaacaac aaaaaaaggc ttattcctag ctggatcttg caaaagacct ctaaacattc
     2821 cagaagccat tgctgatgca agagctgctg cacttcaaat aacacaatat cttaataatt
     2881 tacaatgaaa aactggggat ttaaaatagc aaaaccacgt attatcgacc ttgacacggc
     2941 taatactaaa gcatttgaag agctttgcga aaaagtccct tcggcaaaaa gatgtattat
     3001 gtgtggagct tgtactgcaa cttgttcagc ggctaaccac actagcttta attttcgtaa
     3061 atgcaacata atgtttagaa gaggacaatt tgaaggacta gccgaagaat tagacaagtg
     3121 tatgctttgt ggaaaatgca aactagtttg tccaagagca gtaaacacta gagcttttat
     3181 ttataatatg agaatttttc ttaacgattt gaattataaa aatattaaat aatgaattat
     3241 tcccatttca tattaccttt tagcattgga gcaataatat tatttgtaat aataattgtt
     3301 aaatttacaa aatggtttag acttcttgac cgtaaacaaa aatactttat aaaacgaaat
     3361 attttcacca ctaaaacaat ttctgcaact attgaagcgt tccgcgaagc actaatacac
     3421 agaaatattt ataaaaagaa ccctctttta ggatatatgc acatgagcct ggcttttggc
     3481 tggtttttat taattgtagt tggtaaaatt gaagcttcgg tttattcagg tacatttttt
     3541 gaagagcctt ggcttgctat tttctttaaa tattttgcac gcgaaccaca taatttttgg
     3601 ggagctaaat ttttcacatt tacaatggac tttcttctat taataatact tagcggatta
     3661 gcccttgcaa taattaaaag atttagatca aaaatattag gactaaaaaa agcaacaaaa
     3721 catataattt acgacaaaat agcattaagt gctttatggt taattttccc gctaagatta
     3781 ttagcagaaa gttcaacagc tgcactagta caaaatggag gttttctcac tggaggatta
     3841 ggaagtttat ttccacaaca atttgctcaa gcaatagaat tacctctatg gtgggcatat
     3901 tcaatatcat tatttgtgtt ttttgtgagt ttaccatttt cgcgatatat gcacatacca
     3961 acagaagtag ctttaatatt tttaagaaaa tggggagcaa cagaaggaga acaatattca
     4021 ggattaacaa attttgaaat taattcatgc tcaagatgtg gaatttgtat tgatgcctgc
     4081 cagttaaatt tttctgccaa tataaataat gttcaatctg tatattttat aagagacact
     4141 agatacaata aaatcagcga cttagtagcc aacaattgtt tactgtgtgg cagatgtacc
     4201 gaagcttgtc ctgttggact tgaactcaca caaattcgtc aacaattaag aacaaaaaca
     4261 gaagttcccg gcaaacacta ttatgattat tgtaaagttg aaccaacaaa aaataaaact
     4321 gacgtaatat attttgccgg ttgcatgaca cacttaacac caagtattat tcttgctatg
     4381 aaaaatattt tttcagcagc aaatgaaaaa gtgtggttta tggacgaaga aaaaggaata
     4441 tgttgcggtc gcccactgcg tcaacaagga tttgtacaac aatctaaaga cttagttagc
     4501 aaaaacacaa aattaatttc ttcgtcaggt gctaaattac ttgtatcttc ttgtcctatt
     4561 tgttgcaaat cttttaaaga agaatatgcc ttagatattg aaattatgca tcatagcgaa
     4621 tacattaaaa tgctaattga tgaaaagaga attaaactaa acaagtcaga acttaatata
     4681 gtttttcacg acccttgcga actaggacga ggcttaaata tttacaaaca acctcgacaa
     4741 gttttaagca aagttggaaa acttgtaaat actgaatttg atgaaaaaga ctcactttgt
     4801 tgtggaggaa gtttagcaaa tactattatt gaactcgatt ctcaattaaa aattagagat
     4861 gatgtattac aaatattggc aaaagataat cctgatgttc ttgctactgc ctgtccattg
     4921 tgcaaaaaga cctttacgcg taattataaa ggaaaagtaa tggatattgc ggaaattgtt
     4981 tcaaattttt tatctttgca aaataattca aaaaaagaag gaaaaaaaga agaagaaaaa
     5041 atattaatat cacaataaca ctaaaattaa aaaaatatta tggttgacat ttttgacaga
     5101 ttaaaagaag ctagaggtcc actaggacaa taccaacaaa aagctgaagg ttacttcatg
     5161 ttccctcaat tagaaggtga aattaaacca agaatgaaat ttttaggaaa agaagttctt
     5221 acttggagtt taaacaacta tttaggttta gcaaaccacc ccgaagttcg caaagctgac
     5281 gctgatgctg ctaaagaatt tggaatggct tacccaatgg gtgctagaat gatgtcgggt
     5341 gaaaccaaat atcacaagga acttgaaaat agacttgcta aattcgttaa acgcgaatct
     5401 gcttatcttt taaattatgg ctatcaaggt atggtatcta ttattgatac tttagctact
     5461 agaaaagatg ttatagttta tgattccgaa gctcatgctt gtataatgga cggaatattt
     5521 ttacataaag ctaaaggtgg gaaatcttat gtttatcctc acaacgacat tgaaagatgc
     5581 gaaaaaatgt taaaatttgc cacaaaaaca gctaaagaaa acggtggtgg aattttagta
     5641 attactgaag gtgtttttgg tatggctggt gatttaggaa accttccaga aatagtaaaa
     5701 cttaaagaaa aatataactt cagaatttta gttgacgatg ctcacggatt tggaactatg
     5761 ggagaaactg gagcaggaac agacgaacac tttggcttac aaaaagatgt tgatatttat
     5821 tttgcaactt tcgcaaaagc tatggctgga ataggtgctt ttgtagcttc agacgaagtt
     5881 attgttgacg ctctaaaata caatatgcgt tctcaaattt ttgcaaaatc tttaccaatg
     5941 ccaatggtta ttggagcttt aaaaagatta gatatgctac aaaatcaacc tgaacacaaa
     6001 gaaaaacttt ggaaaattgc taaagctctt caaaaaggat taaaagacaa tggatttaac
     6061 attggaaaca cacaatcacc tgtaactcca gtttatttca gcggttcagt tcctgaaggc
     6121 acaaatattg ttgtcgattt aagagaaaat cacaatattt tctgttctat ggtagtttat
     6181 ccagtaattc caaaagattt gctaatgtta agaattattc caactgcagc acacactcta
     6241 gaagatgtta atgaaactat agaagttttc aaaaaaatca aggcaaatct tgaagcaggt
     6301 gtttataaca ctaaagaaat acaatcaatg aaagttgaat aataatttta taaaaaacaa
     6361 agcggtataa aacttatact gctttgtttg ttaaaaagct aaagctatga aagaatctaa
     6421 atactaccta caatgcgtaa agtgcgaaca cataaccgaa gattttggtg catggttcga
     6481 gcaaaatcaa gtttgtccaa agtgtggtag caaacactcg gaagtttggt ataacagcga
     6541 ctataacgaa ctaccactaa taatgaaagg aaatcctgaa agtttttggc aatattttta
     6601 ttatttacct ctaaatagaa gagaaaatat aattactaga aatgaaggag ccattagttt
     6661 gcaaaaatgg tggttcttag aagattttgc aaaagaaaaa tacggtttaa atttggatat
     6721 ttatgtttat cgtaacgatt taaacggtgg aacgggcact tttaaagatg tggctgctgc
     6781 tttggctgca agtgtattaa aagaatacga cataaatcaa tactgcgttg catctactgg
     6841 taatacagca acttcttatg ctcgttatct ttctatagct ggaattaatt gttcaatatt
     6901 tcttccagaa aatgcattaa agtcttcgga agcaatgatt agtgcctttg gacaaaatgt
     6961 atatagagta aaaggcgatt attcaaaagc aaaagaaatt gctgctgaat atgcaaaaaa
     7021 atacaatatt ttaatttctt ctggaaatat tgacccactt agagttgaag ctaaaaaaac
     7081 tatggttttt gaatggttac gtcaaattgg aaaaatgcct gatgtttaca ttcaagctat
     7141 tagtggcgga acgggaccta ttgccttaga taaaggcgtt agagaaatta agaaaacttt
     7201 cccagaaatt aaaaatccac gtatgataat ggttcaaccc gatgcatgcg acccgatgac
     7261 tcaagcatgg gaaaaagctg aagcaaaaaa tttccctaag aactatgaaa acgattatcc
     7321 aattttagat aatccacaaa ctcaagttcc tactctagca acaggaaacc ccgcaaccta
     7381 ccctattgtt tcaagaatta tcaagaaaag tggaggaact ttcattagaa tgaaagaaga
     7441 aaaacttgtt ccaataggaa gattagttgc atacgaaagg aaacttctat taggaccagc
     7501 ctctgcagtt tgtttaggtg gattttttga agccttagaa aaaaacttac taaaatctga
     7561 tgaaaccata attattaaca taggtgaagg cgtacgtcgt gcaccagaat ttgttgaaca
     7621 aatgatttat acatcaaaag acatttcctc tgttgacgaa tgcaagcctc atcaaattga
     7681 tgactacaga caacaacttt ggaatgatgt tttaaaataa aaaaatacta aaatcataaa
     7741 taatcaaaag cctcatcaaa attggtgagg cttttgttta tgaatataat ctattctaaa
     7801 attcatacaa attttcaaga tatttaataa aatagtttat atttgcataa actattaaat
     7861 tttaaaatga gtaaaagaat aattgttaca ggtggtgcag ggtttattgg aagtaatctt
     7921 tgcgattatt tgctaaatca agaaaatgaa attgtttgta tagacaattt tgatgaatac
     7981 tatccaccta aacaaaaaca aaataatata aaacaagctc ttaaaaatcc aaatttcact
     8041 tttattaatg aggatattaa aaataaaaat aaactttcta aaattataga tggagagttt
     8101 gatgcagtta ttcatttagc agcaaaagct ggagttagat actcaattaa aaatcctatt
     8161 ggttacgaag aaattaatgt tcaaggcaca aaaagcatct tagaattagc tattcaaaaa
     8221 aatattgagc agtttatttt cgcctcatca tcaagcattt atggcaatgc tcctactcca
     8281 ttcgatgaaa attttacaga tttaaagcca ataaatccat atgctcaaac aaaattatta
     8341 tcagaacaaa ttggaaaaga atattctgaa aaatataatt taagatttat ttcattaagg
     8401 tttttttcag tttatgggcc gagattacgt cctgatttgg taatgaataa aattgcaaat
     8461 agtatttaca aaaatgaaaa gttaagaatt tttggagatg gaagtacagc acgcgattat
     8521 acgtatgtag atgatattgt tttgggaatt gacaaagctt taaattataa aaacacaaaa
     8581 tttgaagttt ttaatttagg aaatggaaaa ccagtaaaac tttcagaaat tattcaaatt
     8641 tttgaaaaag aaacaaataa aaaactcaaa ataaataata tagaacctat aaatggcgaa
     8701 tctgaaataa cttgggcaaa taataaaaaa gcaagcgaac tcttaggctt taaatgccaa
     8761 acagatattg aacacggaat aaaaaattat ttaacttggt ataaaaacca ttgtttatga
     8821 aaaaaactat agaagcaaat agcaagatgt ctatatttag ctatctcaaa ttaatttggg
     8881 attataggtt tttttcacat tcccttgcaa taggagaaat aaagaataaa tatgccaaaa
     8941 cttatactgg catttttctt tctttaattc aaagtttaat tggtttagcg gtttattggt
     9001 taatttttgg aattgcaata aaaattgaca caggcaacat tccttatcct gtttttgctt
     9061 taccaggcat aattatttgg caatttttct cttatttggt aggcacatca agcggagcat
     9121 taataagttc tgaacattta atacaaaaac tttattttcc tagaataaac cttacgttag
     9181 ctaaagtttt accaggttta gttgatttag ctgctggatt cattttgttt gtaatattat
     9241 tttttatttt tggattaaca cccaactttg gctggtttta tatttttcct attattttag
     9301 ttttaataat atctggatta gctgtaggtt tatggacaag tattttatcg ctttacataa
     9361 gagatttaag tcaaattata attcaaatta caaactttgt gatttttatt acacctgttt
     9421 tttatccagg aacaatagta ccagaaaatt ttagatttat tttatacatc aatcctgtag
     9481 caggaatcat agaatactca cgtgctgctc ttttctgtac agccataccc gacaacggat
     9541 atttaattgg atttgcaatt gtttttatat tatttatttc agctattttt atttacaaaa
     9601 atttagaaaa gaaaattact gatttattat gattcagcac gaaaaagcca tagtaattaa
     9661 agatttgaac aagaagtatt ataaaaaaaa gcaatcaagt tcaaatttat tcaaaaatat
     9721 aaaatccaat aataatactg aatcattttt tgctttaaaa aacattaatc tagaaattaa
     9781 caaaggcgaa ataattggaa taatcggtcc taatggagct ggtaaatcta ctcttttaaa
     9841 aattttagcc gaagttacac caccaactag tggaaaaata gaaataaatg gaaaaattgc
     9901 atcgatttta gaaattggca ttggctttca acccgattta tcaggttatg aaaatatatt
     9961 tttatctgga cgactttacg gattaagtaa aaaacaaatt aaatctaaat tagaacaaat
    10021 aattgaaatg tttggttttg ctgattttat aaatacacct gtaaaatatt attcatcagg
    10081 aatgtatatg agattagcct ttgcaataat tacaaatata gaagccgaca tttatttgtt
    10141 tgacgaaata ttaagcgtag gggatttggg gtttcagtcg aaagtactct cagaaattga
    10201 aagactaaaa tcaacaggtt ccactatttt aattgtaaca cattctccaa aaaacattta
    10261 tcaaatttgt gataaaatga ttttaataca tttaggagac gtaattttta ctggttctcc
    10321 aaatgaaact attttagctt acagaaatat aatttactcg tcccaaaatg taagacaaag
    10381 ccgaaaaagc aatgtcttaa atacagacat tttaaaaaaa tttaaaagtt ctgatattaa
    10441 acctgaaatt gctgatttta atcttatttt tttttcaatt tctaactcac caaatgaaga
    10501 aggggaaacc ctaagatgtg ataaagatat tttcttaaaa cttatattta attatcacag
    10561 ccctcaagat tttaaactta ttttagtttt acaagatttg caggggaatg caattaacac
    10621 tcattttata aacataaatc tttcacattc aataatagaa aaagaaatag ttttgttaat
    10681 tgaaaaaaac acttttaatg aaatgagtat tatgtttgac attttgattt caagtttaga
    10741 cgataaaata ttaataaatt atagaaattt gtgtgaaact actcttgaaa gtaataataa
    10801 tttgtcaaaa ttaatgggat acataaatat tcctataaaa gaaataaaaa aaacaagaaa
    10861 tgcaaatacc atttaacaaa tttaatagct tttatttaaa aaataaagaa aaaacattgt
    10921 ctaaactaga taaagtctta aactcgggta aatatatcag ggacaaagaa attgattttc
    10981 ttgaagccaa cataaaaaaa cttactaagc gtaaatttac aataactacc tcatcttgca
    11041 cagatgctat atttttagct ttaaaaacaa ttgatataaa acctggagac gaaattattt
    11101 taccttcatt ttcatatatt gcaagtttaa gtccaatttt aatgtgtaat gctaaaccag
    11161 tttttgttga tatttcgcca gagacattaa ctttagactt tgacttaatt aaaaaagcta
    11221 ttacaccaaa aacaaaagct gtaatttttg ttcaactatt tggttattct caagatttaa
    11281 gcgagctaaa atctttttta aaagggaaaa acatcacatt gattgaagat gctgcacagg
    11341 ctttaggttc aaaagtaaac aatttgaatg gtggcgagca aggcgattta agctgtataa
    11401 gttttgatcc gactaaaatt gtttcagcat tcgggactgg aggaattgtg cttacagaca
    11461 atcaagaata ttatgataaa ctcacaaaat taattcacca cggtagaaac caaaatggag
    11521 aatttgaaat tcttggatac aatagtaaaa tttccgaact taatgcatct ataataaact
    11581 tacaattaga aaatttagat tcaattttgg aaagaaacaa taatatcgcc aaccaatatt
    11641 ttgctgagct atcaaataat aaaaacataa aaatattaaa accgaactca aaccaatttt
    11701 caacatatca taaatttgtt attcaaacaa gagatagaaa tgaattgaga aaacatttga
    11761 acaacaatgg tatagaaacc aaaattcact attccacact tttacacgaa caaaaacttt
    11821 taaaaagttt tgaattcaaa acacataatt tgcaaaatag cgaatatgca aaagaaaaag
    11881 ttttgagttt accgatttat cctaacttaa cgaaagaaga aatttcttat atttgtaact
    11941 taataaataa ttattaaaga tatgatacta gttggttgcg gttctgctgg aaaagaaact
    12001 ttagcaatgc ttttagaaca aggtaaaact gataatatat ttttttatga cgaaaataaa
    12061 aatgctgaag attttatttt tgaaaagttt aaagttatca aaaaaactag caaattatta
    12121 gataatttaa aaattgacaa caagttttgt gtttgcatag gaaatcctcg cagaagaaaa
    12181 aaaatgtata ataaattaat tgagttaggt ggcagtccta acaatgttat ttgggataaa
    12241 aacttgtcta tttcagaatt aagccaaaat gcaactataa tacaacccgg agtacatata
    12301 tcttatgaag tgataattgg aaaatcttgc ttaattcata ataacactag tattggacat
    12361 aagactatta ttggtgactt tgtaaatatc ggaccattat gttcacttat tggaccttgc
    12421 acaataggaa atgaaacata tataagcgct ggctctataa ttttgcctaa tgtaacaatt
    12481 gggaacaatg tatatgtaaa agctggaagc attgttaata gagatttaaa ggattttgag
    12541 acattttaac agattaaaaa atgctaaaca agcatcacat aaaagaatat aatacttctt
    12601 taaatcgtaa aggcttaaaa agaatttgct acgcgccata cacatcttta agattttcgc
    12661 aatctggaaa cattattagt tgctgttaca atagaggata tattttaggt aaatttccag
    12721 aaaacactat taaagaagtc tggaatagta aaaagcttaa agcactccaa aaaaacatta
    12781 aaaaaaataa tctaaattta ggttgcgaaa tctgtaaaga cagaatttta ggcaaaactt
    12841 ttactcttac aggatcattc cattatgacg atttgcatta ctattacaag agcaaatacc
    12901 ctgctatgct tgactttgaa atttcaaata cttgtaattt agaatgtatt atgtgtactg
    12961 gcgaaaactc ttctagcatt agggctaata gagaaaatct aacttgtttg tctccaatct
    13021 atgacagtag ttttgttgaa caacttaaag aatttattac acatatcaaa gaggctagat
    13081 ttagtggcgg agaaccattt ttaaataatc tttattatga tatttgggaa ttaatcataa
    13141 aattaaatcc taaagcaaaa attacagttt caacaaactc aacaatttta aataaaaaaa
    13201 taaaagattt actagacaaa gggaatttta ctattgcagt ttcaatagat tcttttacaa
    13261 agtcaactta cgagaaaata aggcaaaatg catcttttga tagcgttgta gaaaatatga
    13321 aatattttta tgaatattcc ataaaacata ataagccttt ttttataaat ctatgtccta
    13381 tgccttataa ttggaaggaa attcctgaaa taattaaaat ttgtaacagc aagagtataa
    13441 aattaatttt ccatactatt gttttcccgc cgaaagaatc gctttgggca ctagatagtg
    13501 agacattagc tgaaatatca aatttttatg atgattttga atttatagca aactcagaaa
    13561 ttgagttttt taattatagc aattttaaaa atttaaaaaa acaaattaaa acttggcatt
    13621 cagaggctat tgaaagagaa aaaaagatta atttattaag ttctttttca tatgaagaat
    13681 tattattagc ttttcaaaaa caccttggag aaaacaatta tgatttttat caacaaatca
    13741 tgcaattgat aaatgatttt gacataaaaa aaagagaaag aataataaac aaattgtttt
    13801 ttttccataa agaagcatta ttttcagaac ttatacacaa taacactgag cgtcttttaa
    13861 taaaattatc aatgttcgat tattaactct atatgtttaa aaaaatattc actaaaaata
    13921 aaatatggaa ccctctaaac aagaaaatca ttaaggattt caataaaact agggaaatat
    13981 cgaagcgaaa gtatctttgc cacgcacctt ttaagtcttt atgtttttcc cattctggcg
    14041 acatcctagc atgctggtat aacaaaatgt atcctttagg aaaatatcct aatgacaaaa
    14101 ttaaagatat atggtttagt gaaagagcaa aaaaattaag agaacatatt ttacataatg
    14161 atttaaattt tggatgttat gattgtaaac gaaacataga aaacaaattt tatcatgcac
    14221 aaagtccata cagatacgat tttttacccg atactacaag taaatatcct gttagtatgg
    14281 attttcagat ttcggaacaa tgtaatttaa aatgtataat gtgtaatggt gaatattcaa
    14341 caagaataag acaagaaaga gaaaataaaa aaatgattca aagcccttat gatgatgtct
    14401 tttacgaaca actgaaagaa tttatacctc atttaaaaga agcatctttt tcgggtggcg
    14461 aaccttttgt tggcagcaag ttttttaaaa tttgggattt gatgattgag ttaaatacca
    14521 acataaagtt atctataaca acaaatggca atgttctaaa cgacaaagta aaatactatt
    14581 taaacaaatt aagttttcat attaccttat ctttagatac aataaatcct gaaatttata
    14641 agaatattag aataggtgga gatttgaata tattcttaca taattttgac tggttttatg
    14701 aatatgcaaa aaacaaatca acatatttta atgttaaaac ttgcgtaatg caacaaaact
    14761 ggcatgactt accagattta gcagagtttt taaataaaag aaatgtacct tttctatata
    14821 ataatgtttt ttatcctcct aatgcatcaa tatggaattt aagttcaaca aaattatcag
    14881 aaattaaaac ttatttagaa aataattttg tatttcataa tcaatccata gttcaatcct
    14941 acaatgccga aacaatgaaa ggtttattaa gacaaattga ttcctggcaa aaacaagcat
    15001 tatattctga atcatttgaa ttaaataaaa tgacaacaaa agaactcgaa aaatttttat
    15061 taaataattt taaaacattt attttaaatg agcaaaaaga aaatataaac tcctaccctt
    15121 tttcatattt agaaatagaa aaaatcatta caaaacttgt ccaagataca aaaaatgaat
    15181 atctggttaa taatggatta atatatttct gcaaatctcc agtagaaaaa ataatttcag
    15241 aatttagaat cagaaactat gaaactataa aagatagatt tatacaagct tgcaaattta
    15301 catgctaaac aatacattta acgtctccac ga
//
LOCUS       DAOPNX010000066        15064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_4680_length_15064_cov_6.151043, whole genome shotgun sequence.
ACCESSION   DAOPNX010000066 DAOPNX010000000
VERSION     DAOPNX010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15064
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4680_length_15064_cov_6.151043"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..560
                     /locus_tag="PKN53_07895"
     CDS             78..560
                     /locus_tag="PKN53_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88907.1"
                     /translation="MKQKTIILLISFLITSISFAQKTSDTTGLYLEVPLFDIPYQIYA
                     TKTTGNFFTSYVNPSMKQSLAMSNNLYGSAHWGINKLTKTKSEFTDILFRNLAAAGFD
                     LFTFYTPLGIGWLHEEYHRAVMTRRKINSFNDMNTFPFGQSLVYVRKVTDENLNLEKL
                     "
     gene            588..1193
                     /locus_tag="PKN53_07900"
     CDS             588..1193
                     /locus_tag="PKN53_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88908.1"
                     /translation="MKKHLIIVLTLGLLISQNIFADSPLTSTAFSKAYKDSKIVQLAS
                     QAEGKLNKELMDYLIDKKNPIELKVALINELSWDFNGKNNSELFSEYLKKNKVKNINK
                     ASADILICYAYLKALDNYFEVDEAITYAQKAKAKNKKKSYTINIICALIEAQKAMDSN
                     WCTVYTLTNDVRIDETLTKDMKEEAVNIIFEYMDIYKDYCS"
     gene            1238..2281
                     /locus_tag="PKN53_07905"
     CDS             1238..2281
                     /locus_tag="PKN53_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88909.1"
                     /translation="MKKYLFITFILLSLCGFSQKKPLWLEASWRESHYPANSFLTGFS
                     QDIRKKREPIAEASERVKNIATGYVAKNLISNIQTVSENYSKSIAHGNNEKLQSTFEM
                     QTKAETDAIITGIKVESYYDKKSKQVYAFAYVNKKEVSGYYKTQISMLIQQVEGHIFT
                     ADQLKQQTEKIKAEAEYNKTTPLFTKIEYAQILLSALDKNIDEESSQIQKSINLRNKV
                     TQALAALAQGPSLFINCDAQLFNISTNMLENKLKAELAKNNCIFVSDSLQADWLISIE
                     AQAEKYNDIYDTYYSYVNATITLQKRYNNQVVYKNELKEKGGSARDYKDAAEKAYIEI
                     ANKISEELIKYIE"
     gene            2283..3152
                     /locus_tag="PKN53_07910"
     CDS             2283..3152
                     /locus_tag="PKN53_07910"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HOD88910.1"
                     /translation="MKKLSFLIITVIIALSTFAQENRVAVFDPEDPNNTGYKDMVREI
                     LSTAIAKSDKYKPVERVLINKVLEENKYQKTGMVKDSEVSELGKQMGANFVCISIIRK
                     IGSNFFITAKLVNVTTAMVESQEHIKTENGVNDLYEKTEELANKLLSIKSETATNIKY
                     ANKSGSSYKLPTVEINYQVYMVLPEDLNGKYTWQQAKNACENLNGFGYDDWFLPEIHE
                     LKTLYENKDAIGGFKQKWTSCFYWSNTSANDMSYSLEPSHFGIGFRKGLFQIPNPYPD
                     YNSLNVRCVRKVK"
     gene            3440..4684
                     /locus_tag="PKN53_07915"
     CDS             3440..4684
                     /locus_tag="PKN53_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88911.1"
                     /translation="MTKTIFHNAPSLITKVFGNNIQLLPSVDELFEIELAYLEFNCLS
                     KEQLIDRSAFIKSIDNEYTRHYLLYSNYNEAINENRSAQTQTYFEAGQFSTGYATHGL
                     FPYRGKFHPQLIKGLLNILGVEKGETILDPMAGSGTTNIEAALMGINSVACDISPFCQ
                     FMTKVKYESLTIDADLLEDTPFDHKSLFDFFKKGNVLQRITEIKDTKKQKIYNLAFLA
                     WLDALGYSKRVVRSNHEQLFEKVLPRYVETVKSTLRNPYFNQCEVGAVEILPDSEALN
                     IGLPDESVDCVITSPPYSFAIDYVENDKDQLDFLGYDTKELKNKLIGLRGKNKTERLN
                     NYFVDMDKVCAEISRVLKKDKYFCMIIGSNTNQTGGIRLEQTIIDSAKKYDLPLVKSI
                     LKPIKGMRNTMKDEYILIFKKI"
     gene            4687..5667
                     /locus_tag="PKN53_07920"
     CDS             4687..5667
                     /locus_tag="PKN53_07920"
                     /EC_number="3.1.21.4"
                     /inference="COORDINATES: protein motif:HMM:NF016443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DpnII family type II restriction endonuclease"
                     /protein_id="HOD88912.1"
                     /translation="MKSVEDKFTTVIQKNTFYFINQKFEEDYEGYINSLKETLLIVKN
                     RVETEGLRKEIFEWLLKEKENGLRALLALTGFSNEYLKRLTTIIRIVDEPELNSLVYK
                     DKWYKEKNPDNIHEWSDSTILKKIQSNEYFRKGLVNLFFEGASIPFLANTIPLFELKK
                     LSISKLNFEIPELIDTIIRYKEKGSYSGMKGNNPEIVIAEILENLGLTYETGDLTELI
                     SNAPDNKRTMDFIIPNKKNPLIIVESSFLATTSSGQGDKSKTEISIDALIKEHYPEAI
                     FIGFVDGIGWYVRKSDLKRMVTAYEDVFTFHKDELKRFEKLLIKTFKSVR"
     gene            5657..6739
                     /locus_tag="PKN53_07925"
     CDS             5657..6739
                     /locus_tag="PKN53_07925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOD88913.1"
                     /translation="MLDKYLNRIIQGDCLELFKNIPDNSVDMTFADPPFNLQKKYTSY
                     KDNLEFQEYLDWCEKWIAEMVRVTKPTGSIFLHNIPKWLTFYATYLNKFAHFKHWISW
                     DAPTAPMGKSLQPAHYGILFYTKEAKGAKIYELRYPHKRDRKQGYLLKDYGGKKDGLH
                     PFGPLVSDVWTDIHRIKHNSKRDPHPCQLPLHLLDRLILLTTDENDIVLDPFSGTGTT
                     AISAKRLGRKYIGFELDKKYAEISQQKLEQVKPNFKIGEIWVSFYLKDIATIRNNDWH
                     DLQQYFNIPNPARSIDFQKTTLKKGIVIPNDFEFNTETKNEVTNKQTKEERIPLSPTK
                     VPDKSKSKVVAYVATKSINKTENIEV"
     gene            complement(6906..7988)
                     /locus_tag="PKN53_07930"
     CDS             complement(6906..7988)
                     /locus_tag="PKN53_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOD88914.1"
                     /translation="MKKVILFLFGLFFFSSLFTQNNDSLLLKEIETLKKQVADNKHVY
                     DKILKSIDDIMWRILFDDIAYVDKVRIVGPARWRAKTEDDRFAKNPLQFYSYVFIPKN
                     VNVKKSYPLIVLPHGGIHADFTTYHTHIVKELLAQGYVVVAPEYRGSTGYGKMVYENI
                     DYGGLENEDVLASRDYIIENYSFVDSTRVGIVGWSHGGMIALMNILRYPDKYACAFAG
                     VPVSDLENRLASHDSSYTEYFTAPYHIGESILENPYEYKRRSPSFYAHLLSKPLLIHT
                     NTNDNDVYVEEVLLMIDSLKAHNKVFEYKVFQDADGGHGFDRIDTKQAVEIRFIIYKF
                     LQAYLKPSKPFKNVADMRKAAYGFGG"
     gene            complement(8027..9352)
                     /gene="tilS"
                     /locus_tag="PKN53_07935"
     CDS             complement(8027..9352)
                     /gene="tilS"
                     /locus_tag="PKN53_07935"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOD88915.1"
                     /translation="MLKKVFEQIDKANIKTGTKLLCAISGGVDSTVMLHVLDDFGFDC
                     IVAHCNFQLRGKASDDDENFVRELAAKFEFKFLCQTFDTYKYAEKTGLSIQMAARDLR
                     YNWFNEMAKQYNCKYIALAHNSDDQVETIITNLARGTGIKGLTGMSFVKDKLFRPLLF
                     TSREEIEAFAEENAIDFKIDETNKSTKYSRNKIRHKIIPLLEEINTSAKSNILKSVKY
                     LTDTEVIMREYVKSCKEKCLNYIGENIEIDLRELKKCVAPETVLFELLLGEGIPKPLA
                     SESINLIDSKSGKTCSFLNITVLRDRNSLIINKNSKIEKFETVFINNFDELKLLEQFK
                     VSAKLVDYQPDLKINKISNFAYIDFEKLVFPLSLRTWQEGDRFKPFGMKNYRKLSDFL
                     KDEKLNLFEKENLKILESDGKIVWLLGLRLDNRFRISDETKKVLILEKT"
     gene            complement(9356..10012)
                     /gene="aat"
                     /locus_tag="PKN53_07940"
     CDS             complement(9356..10012)
                     /gene="aat"
                     /locus_tag="PKN53_07940"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOD88916.1"
                     /translation="MLKLQYIEDLNFPSVEKATEEGLLGYSFDISVEQVLKALTIGIF
                     PWYEEHTYPFLWWAPDPRFVVFPEKAKVSKSLKKSYKNFEYAINKNFEQVIFSCAKIK
                     RKGQEGTWIDRNMLYVYIMLHRLGYGMSFETYFNGDLVGGLYGVNLGGVFIGESMFHT
                     QTDASKSAFVHLVNYCLENNIHIIDCQLHTPLLESLGGEFISRANYSEYLRKFVNVNI
                     "
     gene            complement(10389..11132)
                     /locus_tag="PKN53_07945"
     CDS             complement(10389..11132)
                     /locus_tag="PKN53_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOD88917.1"
                     /translation="MIAYEVLRIIDSKPLFALEHYQRLCNSILNNNRNIKLEYHSFIL
                     EIKNLIDTNNFQLGNIKVEVVFDEVSTDYKVKAFIIPHHYPSDYDYKNGVRLATYLYT
                     RENPNLKIWNQNLRENIDDLIKTQNVFEVVYVNKHNFLSEGSRSNLFFIADNKLISAK
                     PEDILLGVTRKYIIEVAKPLGLELVEKDIPLAEIESYDSAFLSGTSPKVLPVRKINNI
                     YFSSNNKYLRDLMTKFDERLNEDIRNFQF"
     gene            complement(11129..11869)
                     /gene="mtnP"
                     /locus_tag="PKN53_07950"
     CDS             complement(11129..11869)
                     /gene="mtnP"
                     /locus_tag="PKN53_07950"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOD88918.1"
                     /translation="MIGIIGGTGFEKSNIFKNPQVKNIETKYGKPSSPITIGTINDKS
                     VALLSRHGHEHTITPSHVNNRANIEALKLLGCKNIIATTACGSLREEMHPGNFVVLDQ
                     FIDFTKHRINTFYDEFKPGEMQHCPMSDPFSESLRNTIIKAAELCNVNLHKSGTMITI
                     EGPRFSTRAESFMFQKWGADLINMSTAPETALANEANIPYAAIAIVTDYDCWKIDEEP
                     VSIEIVLDNFSKSVTKLTEILIKTISIL"
     gene            complement(11970..12848)
                     /locus_tag="PKN53_07955"
     CDS             complement(11970..12848)
                     /locus_tag="PKN53_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88919.1"
                     /translation="MKILKFNLILVLVMFVSVLNAQVIGGRQTKLEKFYAEERWEDCA
                     FRAERMVLQEKYKNDPEINLYLAASYAKVFLMCLEDSTLLNKVPEYLNAYNYALKYST
                     VAKRKDKKTQIIFPKNNFMLEEIAITGIHYIDHYMSIKRVPKANSYLRKIMKVYSDEN
                     FKFLNGVLSVMTGDTATGNPLIRETFANIDSRKAREELNTDFIMIDAFDYYVNFLMNQ
                     ETPLVDSAKNVANRGVKCFPDCELLKYNLKLIDDPELKAEKPKNEKKKLILKSIVLNS
                     LNENDEDDDVDDEDDD"
     gene            13132..13593
                     /locus_tag="PKN53_07960"
     CDS             13132..13593
                     /locus_tag="PKN53_07960"
                     /inference="COORDINATES: protein motif:HMM:NF025454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HOD88920.1"
                     /translation="MYSKSEAAEIRKNFWMAFDTYSRKHLGPKRKWILYNTGIKDFVM
                     KFEINRQFARVMIAFENKDENKRFDNFLKLKEYELLYIDILGEDWIWDEHFTNDSGKI
                     VCALYKQIDNVNIYRKNDWGIIFEFFALNMLKLEQTFEEVKPFIEDYIKQN"
     gene            13604..>15064
                     /locus_tag="PKN53_07965"
     CDS             13604..>15064
                     /locus_tag="PKN53_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOD88921.1"
                     /translation="MNRKFSFLLSVMMLLSTFSFAQKQIEIEDLYKKGTFRQSYVYGI
                     ESMKDGEHYTNLNMARNSLIKYSYSTGEQVETIFKAKDFESDVIDWIFDYQFSADENK
                     ILISFQYEPIYRHSYLAEYIIYDRTKNTITRLSENGKQQLAFFSPDGNKIAFVRDNNI
                     FVKDISQENSQEVQLTTDGERNKILNGVPDWVYEEEFSYNKAYDWSADSKKIAFLKSD
                     ESEVKTFTFPIFGSLYPKNYEFKYPKAGEDNSKISLHIVDIETKQIIDADMGNLDDMY
                     IPRLMFTQNPEVLAAVKMNRLQNKYELFSIDANTGKSKVILTLTDPKYIEINDDLKFL
                     NDKKHFVYSHESDGFRHLYLYDLNGKLVKQLTSGNWEVTEFYGIDEANNKAYFQAAKV
                     SPLNREIYSVNLDGSNLTMLSKQVGTNSASFSKTFKYFINDWSDANTPNQTCLYNNNG
                     ELIRELEVNKSLSKKISEEYKFAKKEFFTFNLEDGTE"
BASE COUNT         5639 a         2371 c         2178 g         4876 t
ORIGIN      
        1 cttcgattga agtttagtgg tgaaaatcgc caccttcgca aagccgcaaa acgttaactg
       61 caattatata aactaccatg aagcaaaaaa caataatatt actaatctct tttttaataa
      121 catcaattag ttttgctcaa aaaacatctg ataccactgg cttgtatttg gaagttccat
      181 tatttgatat tccttatcaa atatatgcca caaaaacaac aggcaacttt tttactagtt
      241 atgtaaatcc aagtatgaag caatctttgg caatgtcaaa taacttatat ggttctgcac
      301 actggggcat aaacaaactt actaaaacca aaagtgaatt tacagatatt cttttcagaa
      361 accttgctgc tgcgggcttt gatttattta cattttatac tcctttggga attggttggc
      421 ttcacgaaga atatcataga gcagtaatga caagacgcaa aataaatagt ttcaacgata
      481 tgaacacttt tccttttgga caatccttgg tctatgttag aaaagtaaca gatgaaaatt
      541 tgaatttaga aaaattgtaa aaaaataaaa tccaaaaact taaaattatg aaaaaacatc
      601 ttatcattgt cctaacatta ggattgctaa ttagtcaaaa catttttgca gattctcctt
      661 tgacatcaac agcttttagc aaggcttata aagattcaaa aattgtacag ctagcttccc
      721 aagctgaagg taaattaaac aaagaattga tggattattt gattgataaa aaaaatccaa
      781 ttgagttaaa agtagcttta ataaatgaat taagttggga ttttaatggc aaaaacaatt
      841 cagaactatt ctctgaatat ttgaaaaaaa ataaagtaaa aaacatcaac aaagctagtg
      901 ccgacatact aatttgctat gcatatttaa aagcacttga caattatttt gaagttgacg
      961 aggcaattac ttatgctcaa aaagctaaag ctaagaataa gaaaaaaagc tatacaataa
     1021 atattatttg cgcacttata gaagctcaaa aagcaatgga ttcaaactgg tgtacagttt
     1081 atacactaac aaatgacgtt agaatagatg aaactttaac caaagacatg aaagaagaag
     1141 ctgtaaatat aatatttgag tatatggata tatataagga ttattgcagt taaaagttgt
     1201 atatttgtta cttaatttgg gtttaaataa tattaagatg aaaaagtatt tatttataac
     1261 ttttatatta ttaagcttat gtggattttc acaaaagaag cctctttggt tagaggcatc
     1321 atggcgtgaa agccactacc ctgccaatag ttttttaaca ggtttttccc aagacataag
     1381 aaaaaaaaga gaacccatag cagaagcctc cgaaagagtt aaaaacattg caactggata
     1441 tgtggcaaaa aaccttatat caaatataca aactgtatcg gaaaactatt ctaaaagtat
     1501 tgcacacgga aataatgaaa aattacaaag cacatttgaa atgcaaacca aagctgaaac
     1561 cgatgcaatt ataactggca ttaaagtaga aagctactat gacaaaaaaa gcaagcaagt
     1621 atatgctttt gcttatgtaa ataagaaaga agtaagtggc tactataaaa cccaaataag
     1681 tatgcttata caacaagttg aaggacatat tttcacagca gaccaattaa aacaacaaac
     1741 tgagaaaata aaagctgaag ccgaatacaa taaaacaaca cctttattta ccaagataga
     1801 atatgcacaa attttgttat ctgcccttga taaaaatata gacgaagaaa gttctcaaat
     1861 acaaaaaagc ataaatttac gaaacaaagt aacgcaagcc cttgcagcct tagctcaagg
     1921 accaagtttg tttataaatt gtgatgcaca actttttaat atttcaacca atatgttgga
     1981 aaataaactt aaagcagaat tagcaaaaaa caattgcatt tttgttagcg atagtttaca
     2041 ggctgactgg ttaattagca ttgaagctca agcagaaaaa tacaacgata tttacgacac
     2101 ctattattca tatgtaaatg caacaattac gctacaaaaa aggtacaaca atcaagtagt
     2161 ttacaaaaat gagctaaaag aaaaaggagg cagtgccaga gattataaag atgctgcaga
     2221 aaaagcatat attgaaattg caaataaaat aagtgaagaa ttaattaaat atatagaata
     2281 acatgaaaaa gttaagtttt ttaataatta ccgtaataat tgctttaagc acttttgcac
     2341 aagaaaacag agtagcagtt tttgacccag aggatccaaa caatactggt tataaagata
     2401 tggtacgtga gattttgagt acagcaatag caaaatctga taagtacaag cctgttgagc
     2461 gtgttttaat taacaaagta ttggaagaaa acaaatacca aaaaacaggt atggttaaag
     2521 acagcgaagt aagcgagtta ggtaaacaaa tgggagctaa ttttgtgtgt atttcaataa
     2581 taaggaaaat tggcagcaac ttttttatta ctgctaaatt agtaaatgtt actactgcca
     2641 tggtcgaatc gcaagaacat attaaaactg aaaatggcgt aaatgacctt tatgaaaaaa
     2701 cagaagaatt agctaataaa cttttatcca taaaaagcga aactgctaca aatataaaat
     2761 atgcaaacaa atctggttct agctacaaat taccaaccgt agaaattaac tatcaagttt
     2821 atatggtatt gcccgaagat ttgaatggca aatacacatg gcaacaagca aaaaacgcct
     2881 gcgaaaattt aaacggtttt ggatatgacg attggttttt accagaaatt cacgaactaa
     2941 aaacattata cgagaacaaa gatgcaatag gcggatttaa gcaaaaatgg actagttgtt
     3001 tttattggag caacacttct gcaaacgata tgagttacag tcttgaacca agtcactttg
     3061 gcataggatt tagaaaaggt ctcttccaaa ttccaaaccc ctacccagat tataatagct
     3121 tgaatgtgcg ttgtgtgaga aaggtgaagt aggttttggt gggtagaaat aataagaatt
     3181 atagaatttt tttgggggat agtttggaat ttgtaaattt gttacgggaa tttcaagttt
     3241 tacattttat ttccacaaaa cactattacc aaacaaaaag ttatcaatca ttttgttgaa
     3301 aactatccaa atgaatcaac atataattag tataaaagtt gtatttttga caaaacctaa
     3361 aatttatagc aacagataaa catggttttg ttgaatttta aaacaaaaaa atgaaacaca
     3421 taaaccaaaa aataaaaata tgacaaaaac aatatttcat aacgccccct cacttataac
     3481 taaagttttt ggtaacaaca ttcagctttt gccaagtgta gatgagttgt ttgaaataga
     3541 attggcttat ttagaattta attgtctttc caaagaacaa ctcatagatc gttcggcatt
     3601 tataaagtct attgataatg aatatacaag gcattatttg ttgtattcaa attataacga
     3661 ggcaataaac gaaaataggt ctgcacagac tcaaacatat tttgaagcag ggcaattttc
     3721 gacaggttac gctactcacg gtctttttcc atatcgcgga aaattccacc cgcaactgat
     3781 taaaggatta ttaaacattt tgggagtgga aaaaggagaa acaattttag acccaatggc
     3841 gggcagcgga acgacaaata tagaggcagc tttaatgggt ataaattccg tggcttgcga
     3901 tattagtccg ttttgccaat ttatgacaaa agtcaaatac gaatctctga cgattgatgc
     3961 tgacttattg gaagacactc cgtttgacca taaaagttta tttgattttt tcaaaaaagg
     4021 gaatgtttta caacgaatta cagaaataaa agacactaaa aaacaaaaaa tatataatct
     4081 tgccttttta gcatggttag atgctttggg atattcaaag cgtgtagtac gttccaatca
     4141 tgaacaattg tttgaaaaag ttttaccacg ttatgtagaa accgtaaaat caactttgag
     4201 aaatccatat tttaatcaat gtgaagtagg agcagttgaa attttacccg attcggaagc
     4261 tcttaacata ggcttgcctg atgaaagcgt tgattgcgta ataacttcgc caccatattc
     4321 atttgcaata gattatgtag aaaacgacaa agaccaatta gattttttag gctatgacac
     4381 aaaagagttg aaaaacaagt tgataggatt aagagggaaa aataaaacag aaaggctaaa
     4441 caactatttt gtcgatatgg ataaggtttg tgctgaaatt tcgagagtac taaaaaaaga
     4501 taagtatttt tgtatgatta taggctcaaa tacaaatcaa acaggaggaa tacgattaga
     4561 gcaaaccatt attgattctg caaaaaaata cgacttacca ttagtaaaat ctattctaaa
     4621 acccattaaa ggaatgagaa atacgatgaa agacgaatat atattaattt tcaagaaaat
     4681 ataaaaatga aaagcgttga agataaattt acgactgtca ttcagaaaaa caccttttat
     4741 ttcataaatc agaaatttga ggaagattat gaaggctata ttaactcatt aaaagaaacc
     4801 ttgttgattg tcaaaaacag agtagaaaca gaaggactta gaaaagagat ttttgaatgg
     4861 ctactgaaag aaaaagaaaa cggattgaga gctttattag ccttaactgg attttcaaat
     4921 gaatatctaa aacgactaac cactattata cgaatagttg atgagcctga acttaattca
     4981 cttgtatata aagataagtg gtataaagaa aaaaatcctg acaatattca cgaatggtcg
     5041 gatagtacaa ttttaaaaaa gatacaatca aacgagtact ttcgtaaagg cttagtaaat
     5101 cttttctttg aaggggcttc tattccgttt ttagcaaata caattccact ttttgaatta
     5161 aaaaaattga gtatatctaa acttaatttt gaaattccag aactgataga tacaattatt
     5221 cgctataaag aaaaaggaag ttattctgga atgaaaggca ataatcctga aatagtaatt
     5281 gctgaaatac tagaaaattt gggattgaca tacgaaacag gcgatttaac agaattaata
     5341 agcaatgcac ctgataacaa acgaactatg gactttatca ttccgaacaa aaaaaatcca
     5401 ctcattattg ttgaaagctc ttttttagca acaacttcat caggacaagg agacaagtca
     5461 aaaactgaaa tttcaattga tgctttaatc aaagaacact atcctgaagc aatttttata
     5521 ggttttgttg atggcattgg ttggtatgtt cgcaagagcg acttaaaacg aatggtaaca
     5581 gcttatgaag atgttttcac attccacaaa gatgaactaa aacgatttga gaaactatta
     5641 attaaaacat ttaaaagtgt tagataaata tttaaataga attattcaag gcgactgttt
     5701 agagttgttt aaaaatatcc ctgacaattc agtggatatg acttttgctg acccgccatt
     5761 taacttacaa aaaaaatata caagttataa agataatcta gaatttcaag aatatcttga
     5821 ttggtgtgag aaatggattg cagaaatggt gcgagttaca aaaccaacag gttctatttt
     5881 tttacataat attccaaagt ggcttacatt ttatgctact tatctaaaca agtttgctca
     5941 ttttaagcat tggatttcgt gggatgctcc taccgctcca atgggtaaat cattacaacc
     6001 agctcattac ggaattttat tttatacaaa agaagctaaa ggggcaaaaa tttacgaatt
     6061 acgttaccca cataagcgtg atagaaaaca aggatatttg ttgaaagatt atggcgggaa
     6121 aaaagacggt ttacacccat ttggaccttt agtttcagat gtttggacag atatacaccg
     6181 tataaaacac aactccaaac gtgacccgca cccttgtcag ttacctttgc atttattaga
     6241 cagattaata ttacttacaa cagacgaaaa tgacattgtg cttgacccat tctcaggaac
     6301 aggcacaaca gcaatttcgg caaaacggct cggcagaaaa tatataggtt ttgaattgga
     6361 taaaaaatat gccgaaattt cgcaacagaa attagaacaa gtaaaaccga attttaaaat
     6421 cggggaaatt tgggtaagtt tttatttgaa agatatagct acaatccgaa ataacgattg
     6481 gcatgactta caacaatatt ttaatattcc caatcctgct cgttcaatag attttcaaaa
     6541 aactactttg aaaaaaggga tagttatccc aaatgatttt gaatttaata ctgaaacaaa
     6601 aaacgaagtt actaataaac aaacaaaaga agaaaggata ccattatctc ctaccaaagt
     6661 tcctgacaaa tcaaagtcca aagttgtggc ttatgtagca actaaaagta tcaataaaac
     6721 agaaaatata gaagtataaa taattaaaac gatagataga tagacaatca agcaagtaga
     6781 aattgcatat tttaacacca acttgccttc tattaataac gttctatctt tgcaaaaaaa
     6841 cattattggt aaagtaacaa aagtaaaaaa ttcccctcac tcaaccaaat tcacacactc
     6901 tccccctacc ccccaaatcc ataagctgcc tttctcatat ctgctacatt tttgaaaggt
     6961 tttgagggtt ttaagtaggc ttgtaaaaat ttgtaaatta taaacctaat ttcaactgct
     7021 tgttttgtgt ctattctatc aaagccatgt ccaccgtcag catcttggaa aaccttgtat
     7081 tcaaaaactt tattatgagc tttcaaggag tctatcatta acaaaacttc ttctacataa
     7141 acatcgttat cgttagtatt agtatgaatt agcaatggtt ttgaaagcaa gtgtgcataa
     7201 aatgatggag accttctctt gtattcgtat gggttttcta aaatagattc tccaatatga
     7261 taaggagctg taaaatactc tgtataagaa gaatcgtgag aagctaatct gttttccaaa
     7321 tcgctaacag gaacgcctgc aaaagcacaa gcatatttat cgggatacct taaaatattc
     7381 attaaagcaa tcataccacc atgactccaa ccaactatgc caacccttgt gctatcaaca
     7441 aagctatagt tttcaataat ataatctcta cttgctaaaa catcttcgtt ttctaaacct
     7501 ccataatcaa tattttcata aaccattttt ccgtaacctg tgctacccct atattctgga
     7561 gctacaacaa cataaccctg agcaagtaac tctttaacaa tatgtgtgtg ataagttgta
     7621 aaatcagcat gaataccgcc atgaggtaaa actataagtg gataagattt ctttacattt
     7681 acatttttag gaataaaaac ataagaataa aactgtagcg gattttttgc aaatctatca
     7741 tcttctgtct ttgctctcca ccttgctgga ccaacaattc taactttatc aacataagca
     7801 atatcatcaa ataaaatacg ccacattata tcatcaatac ttttgagaat tttatcataa
     7861 acatgtttat tatcagcaac ttgttttttc aaagtttcta tttcttttaa aagtaaagaa
     7921 tcattatttt gtgtaaataa agaggagaaa aaaaataaac caaataaaaa taaaataact
     7981 tttttcataa tattcgtgta aaaggtaata cttagtattt tgtaaattat gttttttcta
     8041 aaatcaaaac tttttttgtt tcatcagaaa ttctaaatct attatctaat ctcaaaccaa
     8101 gtagccatac aattttccca tcagattcaa gaatttttaa attttccttt tcaaataaat
     8161 ttaatttttc atcttttaaa aaatctgata acttacgata atttttcatt ccaaaaggct
     8221 taaacctatc cccttcttgc catgtacgta aactcaatgg aaaaactaat ttctcaaaat
     8281 caatatatgc aaaattacta attttattta tctttaaatc aggctgataa tcaacgagtt
     8341 tggcagaaac tttaaactgt tctagcaatt ttaattcatc aaaattattt atgaaaacag
     8401 tttcaaattt ttcaattttt gaatttttat ttattatcaa actatttcta tccctcaaaa
     8461 ctgtaatatt caaaaatgaa caagtttttc cgctttttga atcaattaaa tttatacttt
     8521 cacttgccaa aggttttgga atcccctcgc ctaacaataa ttcaaataaa acagtttctg
     8581 gagctacaca tttttttaat tcacgtaaat caatttcaat attttcgcca atataattca
     8641 aacatttttc tttacaagat tttacatact cacgcataat aacctctgta tcagtaagat
     8701 attttacaga ttttaaaata tttgattttg ccgaagtatt aatttcttct aatagtggaa
     8761 ttattttatg ccttatttta ttgcgagaat attttgtact cttatttgtt tcatcaattt
     8821 taaaatcaat agcattttct tcagcaaaag cctctatttc ctctcttgac gtaaaaagca
     8881 aaggtctaaa aagtttgtct ttcacaaaac tcattcctgt aagtcctttt attccagtgc
     8941 cacgagctaa atttgtaatt attgtttcaa cttggtcgtc tgaattatgt gctaaagcga
     9001 tgtatttaca attatattgc ttagccattt cgttaaacca gttgtatcgc aaatcgcgtg
     9061 cagccatttg aatagacaag cctgtttttt ctgcatattt atatgtgtcg aaagtttggc
     9121 aaagaaattt aaactcaaat tttgcagcta gttctcttac aaagttttca tcatcatcag
     9181 aagctttgcc tcttaattga aaattacagt gagcgacaat acaatcaaaa ccaaaatcgt
     9241 ctaaaacatg caacataaca gtagaatcta ctccaccact tattgcacaa agtagttttg
     9301 ttcctgtttt tatatttgct ttatctattt gctcgaatac ttttttaagc ataatttata
     9361 tgtttacgtt tacaaattta cgcaaatatt ctgaataatt cgctctagaa ataaattctc
     9421 cacccaaact ttctaaaaga ggagtatgca actgacaatc aataatatga atattatttt
     9481 ccaaacaata atttacaaga tgtacaaaag cagatttgct agcatcagtt tgtgtatgaa
     9541 acattgattc tccaataaaa actccgccaa gattcacacc ataaagtcca ccaactaaat
     9601 cgccattaaa ataagtttca aaagacattc cgtaacccaa tctatgaagc attatataaa
     9661 cataaagcat atttctgtct atccaagttc cttcttgacc tttacgctta atttttgcac
     9721 aactaaaaat tacttgctca aaatttttat ttattgcata ttcaaagttt ttatatgatt
     9781 tcttcaagct ttttgaaact ttggcttttt ctggaaacac aacaaatcga gggtcgggag
     9841 cccaccataa aaatggataa gtatgttctt cgtaccaagg aaaaatgcca attgtcaaag
     9901 cttttaaaac ttgctcaacc gaaatatcaa aactatagcc caaaagtcct tcttctgttg
     9961 ctttttccac agaaggaaaa ttcaaatctt cgatatattg caattttaac atcacacaaa
    10021 attacaaaaa cttaggattg taaaaacagg taagtcaaga aatttaactt tttatactga
    10081 aagaaaattt aaacgataaa attaaaacct ctgctcactc ggatttgtaa tcctagtatt
    10141 ggtaatgttt cactcggatt acaaatccga gcgagcgtca ggggtattgt gagtgattta
    10201 ttaattatca aatgcttgtc cgtaggacaa catgtgatta accgtaggcg gcagcctgcg
    10261 gtaattagac cctcgaaagc cttgcccgta gggcaacaca attctttatt attatgagtg
    10321 ctacaatatg aaaacagtaa gataagtcaa aaaattagct tttctgaaag aaaatttaaa
    10381 caataaaatt aaaactgaaa atttctaata tcttcattta atctttcatc aaatttcgtc
    10441 attaagtctc ttaaatattt attatttgag ctaaaataaa tattatttat ttttcgtact
    10501 ggtaaaactt ttggcgatgt tcctgaaaga aaagcagaat cataactttc aatttcagcc
    10561 aatggaatat ccttttctac caactctaat ccaagtggtt tagcaacttc aataatatat
    10621 tttcgggtta ctcccaacaa aatatcttcg ggttttgctg aaatcagctt gttatctgca
    10681 ataaaaaata aattagaacg agaaccttcg cttaaaaaat tgtgtttatt tacataaact
    10741 acttcaaaaa cgttttgtgt ttttatcaaa tcgtcaatat tctctctaag attttgattc
    10801 caaattttaa gattaggatt ttcgcgtgta tataaataag ttgctaatct cactccattt
    10861 ttatagtcat aatcacttgg ataatggtga ggaataataa aagctttaac tttataatcc
    10921 gttgaaactt cgtcaaatac aacttctact tttatatttc cgagttgaaa gttattggta
    10981 tcaattaagt tttttatctc taaaataaaa ctgtgatatt ccaattttat gtttctatta
    11041 ttattcaaaa tagaattaca caaacgttga taatgctcta aagcgaataa aggtttagag
    11101 tcaattattc gtaaaacctc ataagctatc ataaaataga aatagtttta attaaaattt
    11161 cagttaattt tgttacagac ttgctgaaat tatcgagaac aatttctatt gaaacaggtt
    11221 cttcgtcaat tttccagcaa tcataatctg taactattgc aatagcggca taaggaatat
    11281 ttgcctcatt tgctaaagca gtttctggag ctgtgctcat atttattaaa tctgcacccc
    11341 atttttgaaa cataaaagac tctgctcttg ttgaaaatcg tggaccttca atagtaatca
    11401 tagtaccaga tttatgtaaa ttaacattgc acaattcggc agctttaata attgtatttc
    11461 tcaaactttc tgaaaacggg tcagacattg ggcaatgttg catttcgcca ggtttaaact
    11521 cgtcataaaa agtattaatt ctatgcttag taaaatcaat aaattggtct aagacaacaa
    11581 aatttcctgg gtgcatttct tctctcaagc taccgcaagc agttgttgca attatatttt
    11641 tacaacctaa aagttttaac gcttcaatat ttgctcgatt attaacatga cttggagtta
    11701 ttgtatgctc gtgtccatgt cgcgaaagca aagcaacact tttatcattt attgttccta
    11761 tcgttattgg cgaagaaggc ttgccatatt ttgtctctat attttttact tgtggatttt
    11821 taaaaatatt tgatttttca aaaccagttc cacctattat tcctatcatc tttatctaaa
    11881 tttcttctac acaatcaatt attcaattat caattattca attatcaatt attcaattat
    11941 caattattca attatcaatt atcaattagt taatcatcat cttcatcatc tacatcatca
    12001 tcttcatcat tttcattcaa actatttaag acaatacttt tcagtataag ttttttcttt
    12061 tcgttttttg gtttttctgc ctttagctct ggatcatcta taagttttaa attatatttt
    12121 aacaattcac aatctggaaa gcacttaaca cctctatttg cgacattttt agcactatcg
    12181 acaagtggtg tttcttgatt cattaaaaaa ttaacataat agtcaaaagc gtctatcata
    12241 ataaaatcgg tatttagctc ttctcttgct tttctgctat caatatttgc aaaagtttct
    12301 ctaattaaag gatttccagt tgctgtatcg cctgtcatta cagaaagcac tccatttaag
    12361 aacttgaaat tctcatctga ataaactttc ataatttttc tcaagtatga atttgcttta
    12421 ggaactcgtt ttattgacat ataatggtct atataatgaa ttccagttat tgcaatttct
    12481 tctaacataa aattattttt tggaaaaata atttgtgttt ttttatcttt tcttttcgca
    12541 actgttgaat actttaaagc ataattatag gcattcaaat actctggtac tttgttaagc
    12601 aaagtagaat cttctaaaca cattaaaaaa acctttgcat acgaagctgc caaatataag
    12661 tttatttctg gatcattttt gtatttttcc tgcagtacca ttctttctgc tctaaaagca
    12721 caatcttccc acctttcttc tgcataaaat ttctctaatt tcgtttgtcg tccacctatt
    12781 acttgagcat tcaaaacgct tacaaacatg actaacacaa gtattaagtt aaattttaat
    12841 attttcataa tctataaaaa tttgtggcac aaaatataaa atctttagtt catttcaata
    12901 cgtatttacc gaataattat aaaaacttat taacggcatt tataaacaat tcaaatacca
    12961 ttccaaaatc aataaatgct tttatcttta tccttagcga gttaaccgaa agcaaagttt
    13021 ttaaagattc tctaaaaaac aaaaaataaa taaccaacaa ttattaattc ttaattatca
    13081 attcttaatt ctaaattaat tttcctattt ttgtttttta gttttataat aatgtacagt
    13141 aaatcagaag cggcagaaat tcgcaaaaac ttttggatgg ctttcgacac atactcgcga
    13201 aaacatcttg gtccaaaacg aaagtggatt ttgtataata ctggtattaa agactttgtg
    13261 atgaaatttg aaattaatcg ccaatttgct cgcgtaatga ttgcttttga aaataaagat
    13321 gaaaacaaac gttttgacaa ttttctaaaa ctaaaagaat acgaactttt gtacattgac
    13381 attttgggcg aagattggat atgggacgag cattttacaa acgattctgg aaaaattgta
    13441 tgtgctttgt acaaacaaat tgacaatgtt aatatttata gaaaaaatga ttggggcata
    13501 atatttgagt tttttgcctt gaacatgctt aaattagaac aaacttttga agaagtgaaa
    13561 ccttttattg aagattatat taaacaaaat taaattaaaa gttatgaata gaaaattttc
    13621 ttttttactt tcagtaatga tgctactaag cacttttagc tttgcacaaa aacaaataga
    13681 aatcgaagac ctttataaaa aaggtacttt taggcaaagt tatgtttatg gaattgaatc
    13741 catgaaagat ggtgaacatt acaccaactt gaatatggca agaaattctc ttattaaata
    13801 ttcttacagt actggcgagc aagttgagac aatatttaaa gctaaagatt ttgaatcaga
    13861 tgttatagat tggatttttg actatcaatt ttcggctgac gagaataaaa tcttaattag
    13921 ttttcagtac gaaccaattt accgtcattc gtatttagca gaatatataa tttacgatcg
    13981 cacaaaaaat actataactc gtctgtcaga aaatgggaaa caacaactag catttttctc
    14041 gcctgatgga aataaaattg cttttgtaag agacaacaac atttttgtaa aagatataag
    14101 tcaagaaaat tctcaggaag ttcaactaac aactgacgga gaaagaaata aaattttaaa
    14161 tggagttcca gattgggttt acgaagaaga atttagctac aataaagcat acgactggtc
    14221 ggcagatagc aaaaaaattg cttttctaaa atctgatgag tctgaagtta aaacttttac
    14281 atttccaatt ttcggaagtt tatatcctaa aaactatgaa ttcaaatatc caaaagctgg
    14341 cgaagacaac tcaaaaatta gcttgcacat agttgatata gaaacaaaac aaattattga
    14401 tgctgatatg ggtaatttag acgacatgta tattccacgt ttaatgttta ctcaaaatcc
    14461 agaagtttta gcagctgtaa aaatgaacag acttcaaaat aagtatgaac ttttttcaat
    14521 agatgccaat actggtaagt caaaagtgat tttaacactt acagacccaa aatacattga
    14581 aataaacgac gatttaaaat tcttaaacga taaaaagcat tttgtttatt ctcacgaatc
    14641 agatggtttc cgccatttat acttatatga tttgaatgga aaacttgtaa aacaacttac
    14701 aagcgggaat tgggaagtta cagaatttta tggaatagat gaagcaaata acaaagcata
    14761 tttccaagct gcaaaagttt cgccactaaa ccgcgaaatt tattctgtaa acttagatgg
    14821 aagcaatttg acaatgttat caaaacaagt aggaactaat tctgcaagtt ttagcaagac
    14881 ttttaaatat tttataaatg actggagcga tgcaaacaca ccaaatcaaa cttgcttata
    14941 caataataac ggcgaactaa ttagagaatt agaagtcaac aaatcactat caaaaaagat
    15001 aagtgaagaa tacaaatttg ctaagaaaga attttttact tttaatttag aagacggtac
    15061 agaa
//
LOCUS       DAOPNX010000067        14934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_4733_length_14934_cov_5.499630, whole genome shotgun sequence.
ACCESSION   DAOPNX010000067 DAOPNX010000000
VERSION     DAOPNX010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14934
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4733_length_14934_cov_5.499630"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(280..726)
                     /locus_tag="PKN53_07970"
     CDS             complement(280..726)
                     /locus_tag="PKN53_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88922.1"
                     /translation="MHSDYNRAMRASELRQELGQDLRQEFKQKSGQDLELEQNMELDA
                     EQKLALEQELRQELGRELRQELKKQTRYSEVLEKLSVVALSRKKISELLGEKRISGSL
                     NKILKKLIEDGLIEHTIPDIKNHPNQKFRITNRGVVFLKLLNSVKS"
     gene            complement(764..2239)
                     /locus_tag="PKN53_07975"
     CDS             complement(764..2239)
                     /locus_tag="PKN53_07975"
                     /inference="COORDINATES: protein motif:HMM:NF024685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HOD88923.1"
                     /translation="MSLIIRTLSLYRKFDLCPKIITTNYDTAFEEAVPKLRRNKAYKG
                     RNYELMKHKEKDAIFLIKLHGCAEDADSMVLLPSDYVNLYSKKDNDAERLIFVLENII
                     INNTILFLGIGLGDFQIKNILKKIENLQGKFNQKHFIITLKPLDSSLSFLEPILISDY
                     RQIENIIDNLIEIKREAEENEQLEVKKHEKGIEVTTKKIEELSKQSFSDECNEAKRLL
                     EREALKYYRKGLKYSLTKQFALAMEEYKQAIELKKYYHEAFNGYGFALASLAKTKKEN
                     EMAILCYQAFENFEKAIEIKPDFYDAYFNWGNSLVILAQIKEDKDADMLYNTAFEKYK
                     KAIVIKPDFHEVYYNWGNALITLLEGKEGKEVEKLYEQVINMYKKVVEIKPDFSEALM
                     KLGLASYALAEIKQDKDAEDLYNQTFENFKQAIKYENNAYKFAGFYKLEKEKDKALFF
                     LDKSLSNNEVEVEFVEKDDAWKDYRNDKDFIEILNKYRTKH"
     gene            complement(2196..2540)
                     /locus_tag="PKN53_07980"
     CDS             complement(2196..2540)
                     /locus_tag="PKN53_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88924.1"
                     /translation="MLKNTIKIHPELKKAIEENELVVFVGAGISYELINSKNQKLGDW
                     KNLVACILENLKSNGYEVEHLISLVDKSEPLEVLKLIETNKQQIPQKAICNFVKSFYE
                     FDNKNTFSLQKI"
     gene            complement(2577..3479)
                     /locus_tag="PKN53_07985"
     CDS             complement(2577..3479)
                     /locus_tag="PKN53_07985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HOD88925.1"
                     /translation="MKKVIFISLLAFFALSLNTAFAQNDSIYVMRNGSVIWKYKFSDI
                     DSIIYYNPDNINIPDTPIDTGTLITGTFTDPRDGYEYSWVQIGSQIWMAENLRYLPSV
                     VNILRSSDTIPYCYVHGYYVVNSEPTVEEAKTKANYITYGALYNWTAAMNGAESSDAN
                     PSGVQGICPDGWHLPSDAEWKQLEMYLGMTEEQANGENYWRESNNEGGKLKETSYTYW
                     FGPNIGATNETGFTALPGGTLPDGTGPTLRYRGFWWSATCNTTQHYSGRYFVKVRELH
                     YNERGIFRSDCPKSAAYSIRCVKD"
     gene            complement(3498..4001)
                     /locus_tag="PKN53_07990"
     CDS             complement(3498..4001)
                     /locus_tag="PKN53_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD88926.1"
                     /translation="MKKNKFIKFILAFSCFALYALPVSTQSLYITTDATQEVFLLNII
                     SKLSFAGNNLVVHKTDNSTTVFAISNIKHMSFYDYMGIDLLNKQNNILTIYPNPTSNL
                     LNINLPSDKLGGNLLVLSLDGKTLMNHKLVDSQTTIDINTLPQGIYFCRYIHGTDSET
                     IKIIKQN"
     gene            complement(4442..6253)
                     /locus_tag="PKN53_07995"
     CDS             complement(4442..6253)
                     /locus_tag="PKN53_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOD88927.1"
                     /translation="MKFNNYIFTGFGMSKGKYKITNDDIFYAIEKEFLSGFSKEKVEK
                     SEKFLSFKEKNPNATAFDYFAGNIMGFYERNHVTPFPPTLKKLYYADTSLELGVEAIQ
                     NALKDANILASDVDAWYVSTVSPQEQAPGIAARIKSFLVEDTNHTPAFTLSSGCAGFN
                     VNLEAAISYFETHPQAKNIVIAHSETMSSFLTQRTKFVPYVTFGDAAAAVVLTKTQSD
                     EKFGVININNFHDLHMLDYVGVDHKRNLYMNDSLIKDRAIVNIPNAANKCLEKSNWTS
                     NDIDYFVPHQTGNVILFACAKNLNIDLEKVFLDGQKYYGNVSGATVPLSLCLLKNQGK
                     LVDNSKILSATAGVGGNFGAFTYIHKNLKPSKEPEIRIFENKNILILGASGSIGLKLS
                     EELYNKGANLFLQANKNLKQLSKFKNAKVFTCDFANDLSVEEFINNLKSLNINFDSVV
                     NLASTINADEVYKVNFTAPVKIINSILPQIKTTIFNLGTAAEDVEFSPTPEWISANRA
                     FHGYLASASGEFLKNGIKTIYLQIGFVEDGISKTFDEKAIFKFMLSVRQERKLTVEEV
                     SDIIQKSLLVPKVLRIDYEYENAMVLGRVAYKLEVDI"
     gene            complement(6257..8113)
                     /locus_tag="PKN53_08000"
     CDS             complement(6257..8113)
                     /locus_tag="PKN53_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOD88928.1"
                     /translation="MKDSFIFKGTSFVLGNYEILNDEIEKYVKSHYLEGFDYHRIENS
                     EDYQEFIKKQPKTSAFDYMVGNIMGFYKRFHVVSFPPTTHKYEEAENSLDLCVNAIEK
                     LLEKTKLNGNEIDAWFVSSATQTQFAPGIAEIAKSYFADIDNQAPCYSLTSACVGFNI
                     NLENAIMYFKQNPQAKNILIAHTEVMSALLPNTKDFVPFSSFGDSAAAVILSRVKTEN
                     QEGVVYITNSEDTKMLDFLGADPNGKLFMNPRMVKSRAVPNISNTFNKILKETQWKIE
                     DLKYFIPHQTGNAIVDSVAKKIGIEKNKVYKEIQYNYGNLSGASIPACLDLLLENELK
                     VGDKIITSVAGLGGEFGGFAYIVPQNNTENHSQAELENKTIFITGASGGIGREIAMQA
                     AEKGGNLILHYNSNSEKITKIKNDILSKYKVKIDLVQADLSDKNQTENLVYEIKSKFK
                     NINYLIVTHAITGSLSKASEVSDLEFEKVLDVNYFSIKNLCDICSEIVTESIVIMGSV
                     GEDAQFPGSASYVASKRALRAYAVSLATKVYENKVNCVYYLPGIVEAGMMAKLDKSQI
                     NMSLNNIRQKSMIEVKKIAERILKSAYRMKIPKVRISYESHLKVIKDGYLKY"
     gene            complement(8110..8877)
                     /locus_tag="PKN53_08005"
     CDS             complement(8110..8877)
                     /locus_tag="PKN53_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOD88929.1"
                     /translation="MNYKTIIVETKDNIGYLKLNNPKKLNLLSQELIGELESAINYFS
                     TNYDLKVLIISSVSKDVFSAGGELNEIGAADYDLGLAMCLRLQRVYSGLQNLEIPVIA
                     ALDGYVLGGGLELALHCDIRFAGENTILRLPESDIGLIPAAGGICMFSKYFSLADSAY
                     YLFTGNQIPIEKAWEKGFVQKIYKSDELMIETEKFAKLLCTKSSQSLRAIKKILISSM
                     FLDVNELLEMEAREFSSVLQTDGKERIKQFFEKRNKV"
     gene            complement(8887..10185)
                     /locus_tag="PKN53_08010"
     CDS             complement(8887..10185)
                     /locus_tag="PKN53_08010"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HOD88930.1"
                     /translation="MKKIYRVFVNITILTVIFLTHTNVCAQNSFDKWFKDNGLRIDYY
                     HTGTDKTEQILFKELKFEEKWGGRKNNLIDKFAYGSYFVEVFDLKTKTLIYSYGFCSL
                     FEEWQFTAEAKTISRGFPESLIIPFPKNKVKVELSSRQYEDNSFKKIFEIEIDPADLA
                     INYEKPVNAEVKKIFGKLEPEKALDLVFIGDGYTKNEIDSFYEDVQKIAEYLFETEPF
                     KTNKEKINIWAVAAISLESGTDIPHEKIWKSTALNTNFYTFRSDRYLTTSDYHSVRDY
                     AGLAPCDQICILVNTETYGGGGIYNFWNITSAKNSHRLEVFVHELGHSLAALGDEYYA
                     DEVSYEDIYNKKLEPYQANLTTLVNFDSKWKNMLDENTPIPTPVTKENLKTIGVYEGA
                     GYQKQGIYRPYISCKMKSLGVDFCPVCQKAISEIINFYAE"
     gene            10244..11506
                     /locus_tag="PKN53_08015"
     CDS             10244..11506
                     /locus_tag="PKN53_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOD88931.1"
                     /translation="MDFYNIINNLKTWLASYDLSSSTVNLIASLIVLACIILVSWLAN
                     YITKTIILAIIKKLVKKTKATWDDIMLEQKVFHKLSQLVPILIVYYAIELAFMDYNWA
                     ILLVKKLTIVYLIILIVRIIFSFLNSLDEIYAQNVGAKKGTSIKSIMQVLKIFVLIVA
                     VIITLSYLLNKEVGYFITGLGAMTAVLMLIFKDSILGLVGGVQLTTNDMVRIGDWISM
                     PSKNIDGDVIEVGLNTVKVQNFDKTISTIPPYTLMTESFSNWRGMKEAGGRRIKRSVK
                     IDMSSVKFCDDELLERLSKIHLIKDYIAERSAEIEEYNKTHQYDLSVLANGRRMTNLG
                     TFRMYIIEYLKANENIKQDMTLLVRQLESTQNGLPLEIYTFTASTEWPVYEKIQSDIF
                     DHIFAVLPEFDLRVYQAPSGEDFKSVKS"
     gene            11593..13227
                     /locus_tag="PKN53_08020"
     CDS             11593..13227
                     /locus_tag="PKN53_08020"
                     /inference="COORDINATES: protein motif:HMM:NF019295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOD88932.1"
                     /translation="MKKSITKSFLALFGLIMFANFATTQTIILEEGFENGLPSTWLSI
                     DDDGDGHFWTTDKSTLFSSGSLSFPAHSGSGAIGSGSTYSNNNVWEPLTPDNWLISPD
                     ISGADTMSYWVRSDIGGSSMYDTERYGIYASSTGRNFSDFTMIAYDSTSGLYNSYAQR
                     GVSLPAGTKYLAFRHYNTTDQGAIVLDDIQFINSGSGTSDVNMSRYVELNVQQGQWIS
                     LDLAADAENTGVKVVSGTKDTILTAGTSWTDFKNYYAQAGTMKVYGNINKFDCYNNGN
                     KITGLDASNNTGLINLYCDGNNLSSLDLTNLTVLERLTCYDNNLSSLDLTNLTALEYL
                     NCSGNNFSSLEITNLTALQELFCSENNLSSIKTSGCDNLRYISCYSNNLSACGLDSLF
                     YQLPIRPADDKGRLHIKGIDVMKRGTNTNPGALSCRDTIATNRNWEVWDYDENAPIIN
                     TSYACPYFTLGIEDVGTNNISAKVFPNPVSNNLNIECEEKIDNLELYDALGKMLIRKK
                     NVLDNTSIDVSNLNSGIYILKIRTEKGSGEYKVIVE"
     gene            complement(13349..13422)
                     /locus_tag="PKN53_08025"
     tRNA            complement(13349..13422)
                     /locus_tag="PKN53_08025"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13386..13388),aa:Met,seq:cat)
     gene            13600..13932
                     /locus_tag="PKN53_08030"
     CDS             13600..13932
                     /locus_tag="PKN53_08030"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOD88933.1"
                     /translation="MENKSKKNNISLSIIFAYLKRHYLLSLLALYYFIGIILYVFAKI
                     DILIPCLWYKIFNVKCPGCGLTRAFIHLLSFDFSAAWDANPLIFIVFPLLSFLLIRDF
                     VLFVKHNK"
     gene            13992..14693
                     /locus_tag="PKN53_08035"
     CDS             13992..14693
                     /locus_tag="PKN53_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88934.1"
                     /translation="MKIYFSLILIFSVSFCFAQDQAGFKDMKASNAKAKIVAKQQIND
                     LHNGALLVRLKTGQNTIDALIKSGQTDLAEKRKQKINEENLRIINAFKSEFNFCPVYF
                     FYSNDSKLVSEGKFDEVKFIDEKLQVIDKFKFEFENFLIAEFGEVKGDTTKFYSHSTM
                     QTTDHFSTEPQATYYGGGSTGAKGLIIKDKNFAQLRRPFPFFVKYPFFRKVTKQEIYT
                     VRKMNKNLFIFLKNN"
     gene            complement(14694..14900)
                     /locus_tag="PKN53_08040"
     CDS             complement(14694..14900)
                     /locus_tag="PKN53_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88935.1"
                     /translation="MKITKNILIEDLVENYPISIDYLSKQGIRCIRCGEPIWGTLEDA
                     GIEKGFTTEEIENFVKELKVLANQ"
BASE COUNT         4920 a         2451 c         2094 g         5469 t
ORIGIN      
        1 aaaccaggcg acaaaatgta tctacctcct gaaaaatggg cgactatatg gtaaatctca
       61 gttaaaagtt aaaagttaaa agtcgaaagt ggcggacgcg tctattgctt cgcagagttc
      121 gctttgctca ctcttattag tttttagttt ttagtagtga gtttaaaaac ttcacaatga
      181 tagatagaat ttctttagtg caggagttgt atagacttgc ttcgcagagt tcgcaagctc
      241 actctgctcg caaaggaaag tcgagcctgc aggtggcttt tagcttttaa ctgaatttag
      301 taattttagg aatacaactc cacgatttgt tattctaaac ttttggttgg gatgattttt
      361 aatgtctggt attgtatgtt caattaagcc atcttctatt agttttttca aaattttatt
      421 taaacttcct gaaattcgtt tttcacctaa cagttctgaa attttttttc gagaaagagc
      481 gacaacgctt aatttttcaa gtacctccga atatcttgtt tgctttttta actcttgcct
      541 taattcacga cctaactctt gccttaattc ttgttctaag gctaacttct gctctgcatc
      601 caactccata ttttgctcta attccaagtc ttgccccgat ttttgcttaa actcttgcct
      661 caagtcttgt cctaactctt gccttaattc tgatgctcgc atggctcggt tgtaatccga
      721 gtgcatagta atgttacact cggattttgc aaacttgaaa ctcttagtgt tttgttctgt
      781 atttatttaa tatttcaata aaatctttgt cattacgata atctttccaa gcatcatctt
      841 tttcaacaaa ttcaacttct acctcattat ttgaaaggct tttatctaaa aagaaaagag
      901 ctttatcctt ttctttctct aatttataaa acccagcaaa tttataagcg ttgttctcat
      961 atttaattgc ttgtttaaaa ttttcaaaag tttggttata taaatcttca gcatctttat
     1021 cctgtttaat ttcagctaaa gcatatgagg caagtcctaa tttcattaaa gcttctgaaa
     1081 aatctggttt tatttcaact acctttttat acatattaat aacttgctca tatagctttt
     1141 ctacttcttt cccttctttt ccttctaata aagttattaa agcatttccc cagttgtaat
     1201 aaacttcgtg gaaatcaggc tttataacaa ttgccttttt atacttttca aaagctgtat
     1261 tatacaacat atctgcatct ttatcttcct taatttgagc taaaataact aaagaatttc
     1321 cccagttaaa gtaggcatcg taaaaatctg gttttatttc aattgctttt tcaaagtttt
     1381 caaaggcttg ataacataaa attgccatct cattttcttt tttagtttta gctaaagaag
     1441 ctaaagcaaa tccataacca ttaaaagctt cgtgataata ttttttcaat tcaattgcct
     1501 gtttatattc ttccattgct aatgcaaact gcttcgttaa actatatttc aacccttttc
     1561 tataatattt taaagcctct ctttccaaaa gtctctttgc ctcattacac tcatcactaa
     1621 aagattgttt agataattct tctatttttt tagttgtaac ttcaatgcct ttttcatgtt
     1681 tttttacttc taattgttcg ttttcttctg cttccctttt tatttcaata agattgtcaa
     1741 taatattttc aatttgccta taatccgata tcaaaattgg ctcaagaaaa ctaagactgc
     1801 tatctaaagg tttaagtgta ataatgaaat gcttttgatt aaacttacct tgtaaatttt
     1861 ctattttttt taaaatattt ttgatttgaa aatcgcctaa accaatacct aaaaaaagaa
     1921 ttgtattatt tataataata ttttccaata caaagattaa gcgctccgca tcattatctt
     1981 tttttgaata taaatttaca taatcagatg gcaacaaaac cattgaatct gcatcttccg
     2041 cacagccatg taatttgatt aagaatatag catctttttc cttatgtttc atcaactcgt
     2101 aatttctacc cttatatgct ttatttcttc tcaattttgg tacagcttct tcaaatgctg
     2161 tatcataatt tgtagtgata atcttagggc ataagtcaaa ttttctgtaa agagaaagtg
     2221 ttcttattat caaactcata aaaagactta acaaagttac atatagcttt ttggggtatt
     2281 tgttgtttat tggtttcaat taatttaagt acctctaatg gttctgactt gtctactaat
     2341 gaaattaaat gctcaacttc atatccatta cttttcaaat tttccaaaat acaagcaact
     2401 aaatttttcc agtcgcctaa tttttgattt tttgaattaa taagctcata tgatatccct
     2461 gcaccaacaa ataccactaa ctcattttct tctattgctt tctttaattc tggatgtatt
     2521 tttatagtat ttttaagcat tacatatatt attttaaaaa aatattacca attagtttaa
     2581 tctttaacgc aacgaataga ataagctgct gactttgggc agtccgacct aaatatgcct
     2641 ctttcgttgt aatgtagctc cctaaccttc acaaaatatc ttccactata atgttgggta
     2701 gtgttgcagg tagcagacca ccaaaagcct ctatatcgta aagttggtcc tgtaccatca
     2761 ggcaatgtac cacctggaag agctgtaaag cctgtttcgt tagtagcacc gatgtttggt
     2821 ccaaaccaat aggtataact agtttcttta agttttcctc cttcgttatt actttcacgc
     2881 caataatttt ctccattagc ttgttcttct gtcattccaa ggtacatttc aagttgtttc
     2941 cactcagcat cacttggtaa gtgccagcca tcagggcaaa tgccttgtac tccacttggg
     3001 ttagcatcac tactttctgc cccattcata gctgcggtcc aattatataa agcaccataa
     3061 gttatataat tagcttttgt tttagcctct tcaacagttg gctcagaatt aactacataa
     3121 tagccatgaa catagcaata tggtatagtg tctgaacttc ttaaaatatt aactacactt
     3181 ggcaagtatc ttaggttctc tgccatccat atttgagaac caatttgcac ccatgagtat
     3241 tcgtatccat ctcgtggatc tgtaaatgtt cctgttatta aagtccctgt atctatcggt
     3301 gtatctggga tatttatatt atctggatta taatatatta tactatcaat atcagaaaat
     3361 ttgtatttcc atattacact accgtttctc attacataaa tactatcgtt ttgagcaaaa
     3421 gctgtattta atgacaaagc aaaaaatgct aataagctaa taaaaataac ttttttcatt
     3481 acaatttcct cctattttta attttgtttt ataattttta ttgtttcact atctgtgcca
     3541 tgtatataac ggcaaaagta aatgccttgt ggcaaagtgt ttatgtcaat agttgtttga
     3601 ctatcaacaa gtttatgatt cattagagtt ttaccatcta ggctaagtac gagcaaatta
     3661 ccaccaagtt tatcgcttgg caagttgata tttagcaagt tgcttgtagg gttcggataa
     3721 attgttaata tattgttctg tttatttaac aaatcaatac ccatataatc gtaaaagctc
     3781 atatgcttta tattgctaat agcaaacaca gtggtactat tatctgtttt atgtaccaca
     3841 agattatttc ccgcaaaact taatttgcta ataatattaa gtaaaaaaac ttcttgagtt
     3901 gcatcagttg ttatatataa actttgtgta gaaactggca aagcatataa agcaaaacaa
     3961 gaaaatgcta aaataaattt aatgaatttg tttttcttca taattaaaaa atttaaattg
     4021 ttaaacaata ttgttgatta aaaatcttga tattaccata aaaataatgc ttagtgcaga
     4081 tagcaaatac accgaaaaag tatttgtcaa atgaaaaata ataatatttt tttaaaagct
     4141 tattgagcgt agtcgaaata agcgtggcac taccgggcgt tgagcgaagt cgaaacgcct
     4201 gtgtatgtgt agagagagct gtatttaact taacaaaatt tacaatttta ttaaagccaa
     4261 aaacatcaat attctccaca gtaaaattca tcttttagat aattttttgt caaaggtata
     4321 aattttaatt ttataacaaa agaaaaaaca agaaattttt aaaataaatt taaacaagtt
     4381 aaccccaagc gagcaaagcg agcataattc ttaattattc attcttaatt cttaattagt
     4441 tttaaatgtc aacctctagt ttatacgcca ctcttcctaa aaccattgca ttttcgtatt
     4501 cgtaatcgat tcttaaaact tttggtacta ataaagattt ttgtattata tcagaaactt
     4561 cttctactgt aagttttctt tcttgccgca cagatagcat aaatttaaaa attgcttttt
     4621 catcaaaagt cttagaaatg ccatcttcta caaagcctat ttgaagataa atggttttaa
     4681 ttccattttt taagaactcg ccacttgcag aagctaaata tccatgaaaa gctctgtttg
     4741 cagaaatcca ttctggagtt ggactaaatt ccacatcttc ggctgctgtg cctaaattaa
     4801 aaatggtggt tttaatttgt ggtaaaatgg aatttataat tttaactggt gcagtaaaat
     4861 tcactttgta aacttcatca gcatttattg tgcttgcaag atttacaaca gaatcaaaat
     4921 ttatatttaa gctttttaaa ttgtttataa attcttcaac tgacaaatca ttggcaaaat
     4981 cgcaagtaaa aacttttgca tttttaaatt tacttaattg ttttaaattt ttatttgctt
     5041 gtaaaaacaa attagcacct ttattataaa gctcctcgct taattttaaa cctatactcc
     5101 cactcgctcc gagaattaaa atatttttat tttcaaaaat cctaatttca ggctctttac
     5161 ttggttttaa atttttatga atgtaagtaa atgcaccgaa atttcctccc actcctgctg
     5221 ttgcacttaa aattttagag ttatcaacaa gttttccttg atttttcaac aaacataaac
     5281 ttaacggaac agttgctcca gaaacatttc catagtattt ttgcccatct aagaaaactt
     5341 tttctaaatc tatattcaaa tttttagcac aagcaaataa aatcacattt ccagtttggt
     5401 gtggaacaaa ataatcaata tcattgcttg tccaattgct cttttccaaa catttgtttg
     5461 cagcatttgg aatatttaca atagctctat ccttaatcaa actatcattc atataaagat
     5521 tacgtttatg atcaacgccg acataatcca acatgtgcaa atcatgaaaa ttatttatgt
     5581 ttattactcc aaacttttca tcgctttgtg tttttgttaa aactactgct gcggctgcat
     5641 caccaaaggt tacataaggt acaaacttag ttctttgagt taaaaacgat gacatggttt
     5701 cgctgtgtgc tattacaata ttttttgctt gtggatgtgt ttcgaaataa gaaattgcag
     5761 cttctaaatt tacattaaat cctgcacagc cactactaag cgtaaatgca ggcgtatgat
     5821 ttgtatcttc gacaagaaaa gattttatcc ttgcagcaat tccgggagct tgttcctgtg
     5881 gcgaaactgt actcacatac caagcatcaa catcagaagc taaaatattt gcgtctttta
     5941 aagcattttg gattgcctct actcctaatt ccaaagaagt gtcggcataa tatagttttt
     6001 tcaaggttgg tggaaatggc gttacgtgat ttctttcata aaaacccata atatttcctg
     6061 caaagtaatc gaaagctgtg gcattcggat ttttttcttt aaatgataag aatttttcgc
     6121 ttttttcaac tttttctttg ctaaaaccac ttaaaaattc tttttcaata gcataaaaaa
     6181 tatcgtcatt agtaattttg tatttgcctt tcgacattcc aaaaccagtg aatatataat
     6241 tgttaaattt cattttttaa tatttaagat atccgtcttt tattactttt aaatgtgatt
     6301 cataagaaat tctaactttt ggaattttca ttctataagc agactttaaa attctttcag
     6361 caatcttttt aacttcaatc atagattttt gcctaatatt atttaaagac atgtttatct
     6421 gagatttatc gagtttcgcc atcattcctg cttcaacaat tccgggcaaa taatacacac
     6481 aattaacttt attttcataa acttttgttg ccaaagaaac tgcataagct cttaaagctc
     6541 gtttcgaagc cacatagctt gcagaaccgg ggaattgtgc gtcttcgcct acagaaccca
     6601 tgattactat gctttcagta acaatttcgg aacatatatc gcataagttt ttaattgaaa
     6661 aataattcac atctaaaact ttttcaaatt ccaaatctga aacttcgctt gccttgctca
     6721 acgagcctgt aattgcatga gtaacaataa gatagtttat atttttaaat tttgatttta
     6781 tttcataaac taaattttct gtttggtttt tatcacttaa atctgcttgc actaagtcga
     6841 ttttgacttt atatttgctt aaaatatcgt ttttaatttt agtgattttt tccgaattag
     6901 aattataatg taaaattaaa tttccacctt tttcggcagc ttgcatagca atttctctgc
     6961 ctattccgcc acttgctcct gtaataaaaa ttgttttatt ttcaagttct gcttgagaat
     7021 gattttcagt attattttgt ggaactatat aagcaaaacc gccgaattca cctcctaatc
     7081 cagcaacaga agtaattatt ttatctccaa cttttagttc attttccaaa agtaaatcca
     7141 aacaagcagg aatagatgca ccgcttaaat ttccataatt atattgaatt tctttataaa
     7201 ctttgttttt ctctattcct atcttttttg cgacagaatc aacaatagca tttcctgttt
     7261 ggtgaggaat aaaatatttt aaatcttcaa ttttccactg agtttctttc aaaattttat
     7321 taaaagtatt tgaaatattt ggtacagctc ggctttttac cattcgaggg ttcataaaaa
     7381 gtttgccatt tgggtctgct cctaaaaaat cgagcatttt tgtgtcttcg ctatttgtaa
     7441 tatagaccac tccttcttga ttctctgttt taactctgct taaaatcaca gccgcagccg
     7501 aatcgccaaa actcgaaaaa ggaacaaaat cttttgtatt tggtaacaaa gccgacatta
     7561 cttctgtatg tgcaattaaa atattttttg cttggggatt ttgcttaaaa tacattattg
     7621 cattttctaa atttatatta aaaccaacgc aagcagaagt taaagaatag catggagctt
     7681 gattatcaat atctgcgaaa tatgattttg ctatttcagc aattccaggt gcaaattgtg
     7741 tttgagtggc agaacttaca aaccaagcgt ctatttcgtt gccatttaac ttagtttttt
     7801 ctaaaagttt ttctattgca ttaacacata aatctaagga attttcggct tcttcatatt
     7861 tatgagttgt tggcggaaaa gatacaacat gaaatctttt gtaaaatccc ataatatttc
     7921 ccaccatata gtcgaaagct gaagttttag gctgtttttt tataaattct tgataatctt
     7981 ctgaattttc aattctatga taatcaaagc cttccaaata atgcgacttg acatattttt
     8041 caatctcatc atttagaatt tcataatttc ctaaaacgaa tgatgttcct ttaaaaataa
     8101 aactgtcttt catactttgt ttctcttttc aaaaaattgt ttaattcttt cttttccgtc
     8161 agtctgcaaa acagatgaaa attctcttgc ttccatttct aacaattcgt ttacatctaa
     8221 aaacattgaa ctaattaaaa tcttttttat tgctcttaat gattgcgaac tttttgtaca
     8281 aagtaatttt gcaaattttt cagtttctat cattaattca tcagacttat aaattttttg
     8341 tacaaatcct ttttcccaag ctttttcaat tggaatttgg tttcctgtaa acaaataata
     8401 agcagaatca gccaaggaaa aatatttaga aaacatgcaa attccgcctg ctgccggaat
     8461 tagtcctata tctgattcag gtaaacgcaa aattgtattt tctcctgcaa atctaatatc
     8521 gcaatgtaaa gctaattcca agccaccgcc aagcacatat ccgtctaaag ctgcaattac
     8581 aggaatttct aaattttgca aaccagaata aactctttgc agtcttaaac acattgcaag
     8641 acctaaatcg taatcggcag caccaatttc gttaagttcg ccacctgcag aaaaaacatc
     8701 tttactaaca cttgaaataa ttaatacttt taaatcataa ttagttgaaa aatagttaat
     8761 tgcactttca agctcaccaa taagttcttg acttaacaaa ttaagttttt taggattatt
     8821 taattttaaa taaccaatat tatctttcgt ttctacaatt attgttttgt aattcatatt
     8881 taaaaattat tctgcataaa aattaataat ttcacttatt gctttttgac aaactggaca
     8941 gaagtcaaca ccaagacttt tcattttaca actaatataa ggtctgtaaa ttccttgttt
     9001 ttgataacca gcaccttcgt aaacgccaat agtttttaaa ttttcctttg taacaggagt
     9061 tggaattggt gtattttcgt ctaacatatt tttccatttt gaatcgaaat tcaccaaagt
     9121 tgtaagattt gcttggtaag gctctaattt tttgttgtaa atatcttcat aagaaacttc
     9181 gtctgcataa tattcatctc ctaatgctgc gagagaatgt ccaagttcgt gtacaaaaac
     9241 ttcgagtcta tgtgaatttt ttgcacttgt gatattccaa aaattataaa ttccgccacc
     9301 gccataagtt tcggtattta ctaaaataca aatttggtcg catggagcta agcctgcata
     9361 atctctaaca gaatggtaat cgcttgttgt taaatatctg tcggaacgaa aagtgtaaaa
     9421 atttgtattc agagctgttg atttccatat tttttcgtgt ggaatatcag ttccgctttc
     9481 taaagaaatt gctgcaactg cccaaatatt tattttttcc ttattagtct taaaaggctc
     9541 tgtttcaaac aaatactcag ctattttctg tacatcttca taaaaactat cgatttcatt
     9601 tttagtataa ccatctccaa taaaaactaa atctaaggct ttttctggtt caagttttcc
     9661 aaaaatcttt ttcacttctg catttacagg cttttcgtaa tttattgcta aatctgctgg
     9721 gtctatttct atttcaaaaa ttttcttaaa gctattatct tcatattgac gacttgacag
     9781 ctcaactttt accttatttt ttggaaaagg aattatcaaa gactctggaa atcccctact
     9841 tatagttttt gcttctgcgg taaattgcca ttcttcaaaa agcgaacaaa agccataaga
     9901 gtaaattaaa gtttttgttt ttaaatcaaa aacctctaca aaatagcttc cataagcaaa
     9961 tttgtcaatt agattatttt ttcttccgcc ccatttttct tcaaatttta gctctttaaa
    10021 taatatttgc tcagttttat cagtacctgt atggtaataa tcaattcgta agccattgtc
    10081 tttaaaccat ttatcaaaac tgttttgagc acaaacattt gtgtgcgtta aaaagattac
    10141 tgtcaatatt gttatgttaa caaaaactct ataaatcttt ttcatattta ttattctttc
    10201 tttatttttg ccgacacaaa tttacacaaa taatttaatt gacatggatt tttataatat
    10261 cattaataat ttaaaaactt ggttagcaag ttacgattta agttcatcta cagttaacct
    10321 aattgcttct ttaattgtat tggcatgtat tatattagtt agttggcttg ctaattacat
    10381 tacaaaaaca attattttag caattatcaa aaaacttgtt aaaaaaacaa aagctacttg
    10441 ggatgacata atgcttgaac aaaaagtttt tcataaatta tctcaattag tacctatttt
    10501 aatagtttat tacgccatag aacttgcttt tatggattat aactgggcaa ttttgcttgt
    10561 aaaaaaacta actatagtat atttaataat tttgatagta agaataattt tctctttctt
    10621 aaattcactt gacgaaattt atgctcagaa tgtaggtgct aaaaaaggca caagcataaa
    10681 aagtattatg caagttttaa aaatattcgt ccttatagtt gctgtaataa taacattatc
    10741 atatttacta aacaaagaag taggctattt catcacaggt ttaggagcta tgaccgccgt
    10801 tttaatgtta atatttaaag attcaatttt aggtttagtt ggtggtgtac aacttacaac
    10861 taatgatatg gtaagaattg gagattggat aagtatgcca agtaaaaaca ttgacggaga
    10921 tgttattgaa gtaggtttaa atactgttaa agttcaaaat tttgataaaa ctatttctac
    10981 tatccctcct tacactttga tgactgaaag ttttagcaac tggcgtggaa tgaaagaagc
    11041 aggtggaaga agaataaaac gttctgtaaa aattgatatg tcatctgtaa agttctgcga
    11101 tgatgaatta ttagaacgac tttcaaaaat tcatttaata aaagactata tagcggaaag
    11161 aagtgctgaa atagaagaat ataacaagac tcatcaatac gatttgagcg ttttagccaa
    11221 tggaagaaga atgacaaatt taggtacttt tagaatgtat attattgaat atttaaaggc
    11281 taatgaaaat ataaaacaag atatgacctt gttagttaga caattagaat caacacaaaa
    11341 cgggctacca ttagaaattt atactttcac tgcttcaaca gaatggcctg tttatgaaaa
    11401 aattcaatct gatattttcg accatatttt tgctgtactt cctgaatttg atttaagagt
    11461 ttatcaagca ccaagtgggg aagattttaa atcagttaaa agttaaaagt ttataaagtt
    11521 taaaagtagc acctgcgagt ttatcaaaag gtgctacttt tgttaaatat taataactaa
    11581 aacatgaaaa gcatgaaaaa atcaattaca aaatcattct tagcactatt tgggctaata
    11641 atgtttgcaa attttgcaac aacacaaacg attattcttg aagaaggctt tgaaaacgga
    11701 cttccatcta cttggttgag tatagacgat gatggagacg gacatttttg gacaactgat
    11761 aaaagtacgc tttttagtag tggctcttta tcttttcctg cacatagtgg cagtggtgct
    11821 attggctctg gttctactta tagcaataat aatgtttggg agccactgac accagataac
    11881 tggctaatat ctccagatat ttcaggggca gacacaatga gctattgggt gcgttcggat
    11941 attggaggta gttctatgta tgacacagag cgttatggta tatatgcttc ttcaacagga
    12001 cgcaatttct ctgattttac aatgatagct tatgactcaa caagtggttt gtataattct
    12061 tatgctcaaa gaggagtgag cttgcctgca ggaacaaaat atcttgcttt ccgacactac
    12121 aacactaccg accaaggagc aatagttttg gacgatattc aatttattaa tagtggtagt
    12181 ggtacttcag atgttaatat gtcccgctat gttgagctta atgttcagca aggacagtgg
    12241 ataagtcttg accttgctgc tgatgctgaa aatacagggg taaaggtagt aagtggaaca
    12301 aaagatacca ttcttacagc aggcacaagc tggacagact ttaaaaacta ctatgcacaa
    12361 gctggcacaa tgaaggtgta tggaaatatt aataagtttg attgttataa taatggtaat
    12421 aaaataacag gattggatgc ttccaataat actggattaa ttaatttgta ttgcgatggt
    12481 aataatcttt cttctttaga cttaaccaac cttacagtgt tggaaagatt gacttgctat
    12541 gataataatc tctcttcttt agacctaacc aaccttacgg ctttggaata tttgaattgc
    12601 agtggtaata atttctcttc tttggaaata actaacctta cggcgttgca agaattgttt
    12661 tgctctgaaa ataatctctc aagtattaaa acaagtggtt gtgataatct aaggtatatt
    12721 tcctgctata gcaataatct ttctgcttgt gggcttgatt ctctttttta ccaattgcca
    12781 ataagacctg cagatgataa aggaagatta catataaaag gaatagatgt aatgaagaga
    12841 ggaacaaata caaacccagg cgctttgtct tgccgagata ccatagcgac taatcgtaat
    12901 tgggaggttt gggattatga tgaaaatgca cctattatta acacaagcta tgcttgtcct
    12961 tatttcactc ttgggataga agatgtagga actaataata taagtgctaa agtttttcct
    13021 aatcctgtta gcaataactt aaatattgaa tgcgaggaaa aaatagataa tttagagctt
    13081 tacgatgcac taggcaaaat gcttatccgc aagaaaaatg ttttagataa tacttctatt
    13141 gatgtttcaa accttaatag cggaatatat attctaaaaa ttcgcactga gaaaggaagt
    13201 ggggaatata aggtgatagt tgagtagaaa gtttataaag tttgaaatgt ttatagtatt
    13261 tcgcacatgc taaatcctag cgagcaaaat aaataatgga ataataaaaa aaagcagttg
    13321 caaaattttc acaactgcct tttttctgta gcgagaggcg ggcttgaacc gccgacctca
    13381 tgattatgaa tcatgcgctc taaccagctg agctacctcg ccatttttta tcaccaaaaa
    13441 tgtgagtgca aaaataagca ttttacttaa ttatccaaca aattattaat cttttttatt
    13501 aaaattttaa ttacagagct taataacaca aatttattca agtaaaagtc gcattaatta
    13561 atttaaaaaa tgtatttttg cttgatttaa ttgattttaa tggaaaacaa gtcaaaaaaa
    13621 aataatattt ccttatcaat aatatttgcc tatcttaaaa ggcattattt gctatcatta
    13681 cttgctctct attattttat aggtataatt ctttatgtat ttgcaaaaat agatatttta
    13741 attccttgtc tgtggtataa aatatttaat gtaaaatgtc caggttgcgg acttacaaga
    13801 gcttttattc atttattaag ttttgatttt tctgccgcat gggatgctaa cccgctaatt
    13861 ttcattgtat ttccactact aagtttttta ctaattagag attttgtact atttgtaaaa
    13921 cacaataaat gaaattttta aatcataata aatatcaaat aataccgtta tttttcaaaa
    13981 ttagtttgtc tatgaaaata tattttagtt taattttaat atttagtgta agtttttgtt
    14041 ttgcccaaga ccaagctggc ttcaaggata tgaaagcctc taatgcaaaa gctaaaatag
    14101 tcgcaaaaca acaaataaat gatttgcata atggtgctct tttagtaaga ttaaaaacag
    14161 gacaaaacac catagatgca ctgataaaat caggtcagac agatttggca gaaaaaagaa
    14221 aacaaaaaat aaatgaagaa aatttgagaa taattaacgc ttttaaatct gaatttaact
    14281 tctgccctgt ttatttcttc tacagcaatg attctaaact tgtttcagaa ggaaaatttg
    14341 atgaagtaaa atttattgac gaaaaattac aagtaataga taagtttaaa tttgaatttg
    14401 aaaacttttt aattgctgaa tttggcgaag ttaaaggcga taccacaaaa ttttattctc
    14461 atagcactat gcaaactaca gaccactttt ctacagaacc acaagcgaca tattatggtg
    14521 gcggaagcac aggtgcaaaa ggattaatta taaaagacaa aaattttgcc caattaagaa
    14581 gaccgtttcc attttttgtg aaatatcctt tttttagaaa agttacaaaa caagaaattt
    14641 atactgttag aaaaatgaat aaaaatttat ttattttctt aaaaaacaat tgattactga
    14701 tttgctaaaa ctttcaattc tttaacaaag ttttcaattt cttctgttgt aaaacctttt
    14761 tctataccag catcttctaa agtaccccaa attggttctc cacatctaat gcaacgtatt
    14821 ccttgttttg acaaataatc aatggaaata ggataatttt ccactaaatc ttcgattaaa
    14881 atattttttg ttattttcat ttttataaaa cagaaataaa taaattttgt ttaa
//
LOCUS       DAOPNX010000068        14311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_4973_length_14311_cov_6.175295, whole genome shotgun sequence.
ACCESSION   DAOPNX010000068 DAOPNX010000000
VERSION     DAOPNX010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14311
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4973_length_14311_cov_6.175295"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..3392
                     /locus_tag="PKN53_08045"
     CDS             201..3392
                     /locus_tag="PKN53_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88936.1"
                     /translation="MTFGILLAFASVLQAQFYNGHQMKFGKNRVQFDEFEWYYFRFQN
                     FDTYFVAGSKDVALKASSIANKNLSEIETFLEHQLKKRIVFVIYKNQSDFRQSNIGLN
                     TGDEQYNIGGVVKVVDNIAFIFVEGDLKSLERQVKSAIANVFLNELLYGDNITSKIAN
                     NTLISPPEWYFQGLLRFVSQKWDSEIDNLVRNAIADNKFDNLNHLNGDDAAIAGHAIW
                     NYIATVYGKQVIPNIVYLSRVTKSTESAFLYVLGFTLENLQDAMVDFYKAQYTSFAYT
                     AETPSGRSILPKTRKHLKYYQVQHSANGEKIAWVENHLGKYKIKYLDLKTNKKKKILR
                     REHVLDQIPDYSYPVLRWHPSGKMLGFIIEKKGSTYYTTYNLETKEFKEIEVHLEKIT
                     SFDYSHDGLYLVLSAFHHGQSDIFVFNIIANTIENITQDVADDYQPVFVNKSQQIVFS
                     SNRKEAELGNKKSNKVEVQENKDIFLYDVKTKTIEQITNTETVNEEFPDLKNKNFTFI
                     SDENGIKNLYAANIDSAINFVDTTTHYRYFLNQTQLTNYPNNILEYNPDKFSNSNAML
                     YKNKNRFQLLYNENLPNNPLKPEKTAWRKYHENQLKKVVISAKQQEIPETDSLSDTSQ
                     GSDIQIIDINNYEFDEKVLKANKIESEEFDKDGNAKDPRTLKYHTTFYTNYLVSQIDF
                     GFLSNSYQTFTGSAFYFNPGFNMILKLATYDLFEDYRITAGFRFAGNFDSNEYLLSFE
                     NLKKRWNKQLVLHRQALNNYAYDYYFKSYTHEAFYILRYPLSQVDAFQFTFNLRQNHY
                     SLLAIDFNSLKQPDFYDYWAGAKAEYIFDNTKALSTNILSGTRFKVFGESYVEMHKRL
                     TDLFVTGYDFRYYKPIHKNFIFAFRVAGSASFGTAKLIYYLGGMDNWINLSAKNPTFN
                     QDIKIDPEVNYVYQAVATNLRGFNQNIRNGTNFTVVNAELRLPVVTYFYKKPLNNNFL
                     KDFQVIAFFDIGSAWSGLSPFSGNNAYENDYYNDFPVSVIVYNNNFPIVSGYGFGLRS
                     KLLGYFVRADWAWGINGDKIQPRMFYLSLSTDF"
     gene            3426..4604
                     /locus_tag="PKN53_08050"
     CDS             3426..4604
                     /locus_tag="PKN53_08050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOD88937.1"
                     /translation="MKNLKDKIQKLNEKYFSEIVEFRRHIHQYPELSFKEFETAKYIK
                     SILTKYNIEFDESFGENAVIGIIKGDLSGDNIALRADMDALPIEELVDSEFKSKVPGV
                     MHACGHDAHVASLLGTAIILQELRNYIKGNIILIFQPAEERNPGGAKILIDKGLLKKY
                     NIIKVLGQHVLPELEVGKFNFGSNYVMATSDEIYIKFSGKGGHAALPHKRSDTVLALI
                     HFINEVNEFKIKLKSDMPIIIAFGKLSAQGSINVVPEISFAEGTMRTFDEKLRNEIKA
                     KLQVIADKYADYYGCKADLEIRHGYPAVYNAPKLNSQVMDLAKDFLGEENVGELEIRM
                     TAEDFAFYSHEVPSVFYRAGVAGNGKGIAGQHTSYFDIDENVFLKSSALMAWFTLNIQ
                     "
     gene            4606..5136
                     /locus_tag="PKN53_08055"
     CDS             4606..5136
                     /locus_tag="PKN53_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOD88938.1"
                     /translation="MRKLPIEEIGRLSAEEFAKTEKLPLIVVLEDIRSHHNTGAIFRT
                     MDAFAGEAVYLCGITGTPPHRDIHKTALGATETVNWKYFNSALEAVEQLRQENYKIIA
                     LEIAEGSLDIQDLEICKTEKIALVLGNEVYGVDQKILDICDLCIEIPQYGTKHSLNVS
                     VSGGIAIWEICKRMLA"
     gene            5246..5968
                     /gene="purQ"
                     /locus_tag="PKN53_08060"
     CDS             5246..5968
                     /gene="purQ"
                     /locus_tag="PKN53_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOD88939.1"
                     /translation="MKFGVLIFPGSNCDHDMIYVLRDILNQEVVELWHKDTDLQDCDA
                     IAVPGGFTYGDYLRSGALARFSPIMKKVVEFAHNGGLVFGVCNGFQILCEAGLLPGTL
                     LHNENQKFICKNQYIKVENTDTAMTMFVDKDKALKIPIAHGQGRYYAPDSTLQEMLQN
                     GQIVWRYCDEDGKLNQKANPNGSVMNIAGVCNVEKNVFGMMPHPERAADPELKNTHGL
                     IIFKSMIEYFNDQKMLEKKEMN"
     gene            6040..8931
                     /locus_tag="PKN53_08065"
     CDS             6040..8931
                     /locus_tag="PKN53_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88940.1"
                     /translation="MALSWNEIKDRALKFSNEWADASSEEADAKPFLVEFFNVFGISR
                     KRVGTFEHRVKKLDDRDGYIDMLWKGTILIEMKSRGKNLDKAYQQAVDYTQGLKQYEL
                     PKYILVSDFENFRLYDLEDNTEIEFKLNDLVNNVQNFGYLLGYQRKVYKAEDPANIEA
                     AELMGKLHDRLKEIGYSGHPLEVYLVRLLFCLFAEDSTIFEKQQFQDYIEQRTNIDGS
                     DLAAKLQELFQVLNTPKDKRFKNLDEQLVEFPYVNGKLFEEILPLASFDSKMRQALLD
                     CTYIDWSKISPAIFGSMFQSVMNPKERRNLGAHYTSETNILKLIKPLFLDELQAEFEK
                     LKGNKNKLKEFHKKLGSLKFLDPACGCGNFLIITYRELRLLEIEVLKELYGTQQVFDI
                     GQIMLVNVDQFYGIEYEEFPARIAEVAMWLIDHQMNMLVSNEFGQYYVRLPLMKSACI
                     MVGNALQMDWSTLTKNTEIDINANTTNIIMVSEPEIHYDTVNIFSKKVQFFDGTYPKS
                     FSGKPHFDYILGNPPFVGKKEQNHEQKLDMQRVFSGVKGFGVLDYVTAWYVKAAQLIA
                     NTSTKVAFVSTNSITQGEQVGILWNLLFNHYNIKIHFAHRTFRWSNEAKGNAAVHVVI
                     IGFANYDTNNKKIFEYEDINSEPHEIKAKNINPYLVEGRDLVVTNRNQPICHVPKMNY
                     GSMPIDNGHLILTNEEKEIAIKNEPFIDELIRPYTGGYEFINNIQRWCLWLQNVNPTV
                     LKASKFVLDRIDKTREFRLQSKRVATNKLAETPMLFGEIRQPDSNYIIIPKVSSENRK
                     YIPIGILSPSIIVSGSALIIPNANNYIFGVMTSSMHMHWMRTTCGRMKSDYQYSAGIV
                     YNNFPWPENPSEKQIKEIEEAAKKVLDTRLQFPNSCLADLYDPLTMPPALVKAHNELD
                     KAVDLAYRPQPFTSEANRMVYLFELYEKYTVDLFAKAKIKKTRKAKNND"
     gene            8936..10024
                     /locus_tag="PKN53_08070"
     CDS             8936..10024
                     /locus_tag="PKN53_08070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HOD88941.1"
                     /translation="MKTCNKCLLDEKYPQLTFNDKGVCSLCSSNKVFKPIGEKALLEI
                     FEAAKSKNKEYNALVPLSGGKDSTYILHLAVNVYKLKVLAMTYDNGLFSQLALDNIER
                     AIKITGVKHIFSKPDFDVQKKIYQNMLRQTGDICGACDIATKTHTLKVARDFSIPIIL
                     YGTSPLENDSFIPDSIQDIERFKYIMKISGNLTKKQINDFLIYPNLNMLRHSIYKKIG
                     IFSKEVSPLFYIDNPTDQEMGEIIKKEMGWQDETSREYSKHLDCIAESLTNYIRNKIY
                     GYERRKCQYSNMIRRNEITRDEAMRLFNADKIEEKPSNYSTVLNHLELTEDDINKAIS
                     YKPLTFENRTSKMNRLQTYLMKKIRNKG"
     gene            10125..11702
                     /locus_tag="PKN53_08075"
     CDS             10125..11702
                     /locus_tag="PKN53_08075"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOD88942.1"
                     /translation="MGWSSWNNYGHNINDSIIKAQADAMVYSGLADLGFKYINIDDGF
                     FDGRNADGSLRINATKFPYGMKTLADYIHSKGLKAGFYSEAGANTCGSIWGAQTGGVD
                     GGLYNHDQQDIDSIFKSWGFDFLKVDFCGGQQQGLDEETRYTQIKTAINNTGRTDINF
                     NVCRWQFPGAWVCSVADSWRMSGDINLTPGSIPSWNEILKIIDQNTFLAAYASPGHYN
                     DMDMLEVGRGLSFEEDKSHFSIWCILSSPLVLGNDLANMSEQTKSILTNPEVIAVNQD
                     TTGLQARRIWDNGSGLQVWAKNLNGKLSNERAVVLFNRRDTAASISVKWKDLNLEGPA
                     TVRDLWERRDLRIMDSMFTADVPSHGVVMLKVVGKQNKLQQVFEAEYAWINTFNLTKN
                     SKILPNQGRAVVDTLCSGLAKVAWLGNSADNYIEFRDVYVYSDGKYKLSISYISGENR
                     NITLSVNGIDKVLKKLNSGDWKTIDNKTITVKLNKGYNTIRLSNANGWMPDVDKIQLK
                     VKSRKSKVEPSLRAHRR"
     gene            12023..12748
                     /locus_tag="PKN53_08080"
     CDS             12023..12748
                     /locus_tag="PKN53_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88943.1"
                     /translation="MFKNYPKTRIELPEKLEAIYNQHYKSNRDGDTTASGLAQKMEAW
                     LHKKVAADVKGSHDKKTLEIGAGTLNQLKYEQSSHYDIVEPFSELYKNSPYITQIKNI
                     YNDIDEIDISQKYDRITSVATFEHILDLPKVVAKTCLLLNPNGSLRTSIPNEGTFLWT
                     LGWKLTTGLEFKIKYGLDYGILMKHEHVNKADEIEEVLNYFYKKNKCYTFGLCKKVAF
                     YRFYESKEPNIELAQKYLESLDK"
     gene            complement(12904..13125)
                     /locus_tag="PKN53_08085"
     CDS             complement(12904..13125)
                     /locus_tag="PKN53_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOD88944.1"
                     /translation="MYWTLELASKLEDAPWPATKDELIDYAIRSGAPLEVIENLQEIE
                     DEGDIYESIEDIWPDYPSKDDFFFNEDEY"
     gene            complement(13213..13761)
                     /locus_tag="PKN53_08090"
     CDS             complement(13213..13761)
                     /locus_tag="PKN53_08090"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOD88945.1"
                     /translation="MKIYTKVGDKGNTSLLGGQIVRKDSLRIEAYGTIDELNSFIGML
                     LNFKIDNSDKEIINFVQNRLFDIGSILAADEKNAKYKLPQLRHITNEDISTLEQAIDK
                     NSENLPKLKSFVLPAGSEFISWCNISRTVCRRAERLIVSAKDEIFIPDEIFIFINRLS
                     DYLFVLGRKYAIVYNIDEILWK"
     gene            complement(13779..>14311)
                     /locus_tag="PKN53_08095"
     CDS             complement(13779..>14311)
                     /locus_tag="PKN53_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD88946.1"
                     /translation="LTSGQYELNGTDVSFMSDNELAEIRNKEIGFVFQTFNLLPRYTA
                     FENVVLPLVYAGVSSKERQKRAQEVIESVGLTDRAHHKPNELSGGQRQRVAVARALVN
                     KPSIILADEPTGNLDTKTSIEIMTLFEEIYKKGNTIILVTHEEDIALYARRIVRLRDG
                     LIESDEINKNVTMDLS"
BASE COUNT         5169 a         2008 c         2498 g         4636 t
ORIGIN      
        1 tagttgatga agaaagactg aaaatgattt gtgcttaggt cgaactcacg ttaataacaa
       61 ttattttttt catatcttgc gtttttatta aaattgcaac aaagataaaa aagagaatta
      121 atatattgcc aaaattattt ttaatttttg tattttgcga acatattttt taacatagtt
      181 tgaaaagttt tataaaaata ttgacatttg gaattttgtt agcatttgca agtgttttgc
      241 aggctcagtt ctataatggt catcaaatga aatttggaaa aaatcgagtt caatttgatg
      301 agtttgaatg gtattatttc cgttttcaga attttgatac ttattttgtt gctggaagta
      361 aagatgttgc cttaaaagct tcgagtattg caaataaaaa tttatcagaa atagaaacat
      421 tcttagaaca tcaactaaaa aaaagaattg tttttgttat ttataaaaat caatctgatt
      481 ttcgtcagtc aaatattgga ttaaatactg gagacgagca atataatatt ggtggagtgg
      541 taaaagtggt ggataatatt gcttttattt ttgtggaagg cgatttaaaa agtttggaaa
      601 gacaagtaaa atcagctatt gcaaatgtgt ttttgaacga attgctttat ggcgacaata
      661 ttacttcaaa aatagcaaat aatactttaa ttagtccgcc agaatggtat tttcaaggac
      721 ttttaagatt tgtttcacaa aaatgggatt ctgaaattga taatttggtt agaaatgcaa
      781 tagcagacaa taaatttgat aatttgaatc atcttaatgg agatgatgcg gcaattgcag
      841 gacatgctat ttggaattac atcgcaacgg tttatggaaa acaagtgatt ccaaatattg
      901 tgtatttaag tagagttact aaaagtacag aatctgcatt tttatatgtt ttaggattta
      961 ctttagaaaa cttgcaagat gcaatggtag atttttacaa agctcaatat acaagttttg
     1021 cctacacagc cgagacacca agtggaagaa gcattttacc caaaactcga aaacatttga
     1081 aatactatca agttcaacat agcgctaacg gcgaaaaaat tgcttgggtt gaaaatcatt
     1141 taggaaaata taaaatcaaa tatttagatt taaaaactaa taaaaagaaa aagattttaa
     1201 ggcgagagca tgtcttagac caaattccag attattctta tccggtttta cgttggcatc
     1261 caagtggtaa aatgttggga tttattatcg agaaaaaggg aagtacttat tacacaactt
     1321 ataatttgga aacaaaagag tttaaagaaa ttgaagtaca tttagaaaaa atcacttctt
     1381 ttgattactc acacgatggc ttgtatttgg tgctttcggc tttccatcac ggacaatcag
     1441 atatttttgt atttaatatt attgcaaata caattgaaaa tatcactcaa gatgttgctg
     1501 acgactatca acctgtgttt gtaaataaat ctcaacaaat tgtatttagt tctaatagga
     1561 aagaggctga acttggaaac aaaaaaagca ataaagttga agtgcaagaa aacaaagata
     1621 ttttccttta tgatgtaaaa acaaaaacca ttgagcaaat aactaatact gagacggtta
     1681 atgaagaatt tcctgattta aaaaataaaa atttcacatt tatttctgat gaaaatggaa
     1741 ttaaaaattt atatgctgcg aatattgatt ctgccattaa tttcgttgat actacaacac
     1801 attacagata ttttttaaat caaactcagc ttacaaatta tcctaacaat attttagaat
     1861 ataatcccga caagtttagc aattctaacg ctatgttgta taaaaataaa aatcgtttcc
     1921 agttattgta caatgaaaat ttgccaaata atccgctaaa acctgaaaaa acagcatggc
     1981 gaaagtatca cgaaaatcag ttgaaaaagg ttgttatttc tgcaaaacaa caagaaatac
     2041 ctgaaactga tagtttaagt gatacatctc aaggttcaga tattcagatt attgatataa
     2101 ataattatga gtttgatgag aaagtgttga aagcaaataa aattgaaagt gaagagtttg
     2161 ataaagatgg gaatgcaaaa gacccacgaa cattaaaata tcatactact ttttatacaa
     2221 attatttggt aagtcaaata gactttggat ttttaagcaa ttcgtatcaa acttttacgg
     2281 gttcagcttt ttattttaat ccaggattta atatgatact gaaacttgca acttatgatt
     2341 tgtttgaaga ttacagaatt acagcaggct ttagatttgc tggtaacttt gatagtaacg
     2401 aatatttatt gagttttgaa aatcttaaaa aacgttggaa taaacagttg gttttgcata
     2461 gacaagcgct gaataattat gcttatgact attattttaa atcttacact cacgaagctt
     2521 tttacattct gcgttatcct ttgtcgcaag tagatgcgtt tcaatttaca tttaatttac
     2581 gacaaaatca ttattcatta ttggcaatag attttaatag cttgaaacag ccagattttt
     2641 atgattattg ggcaggagca aaggcagagt atatttttga taatacaaaa gcacttagca
     2701 ctaatatatt gtctggaaca agatttaaag tgtttggcga atcttatgtt gaaatgcaca
     2761 aaagacttac cgaccttttt gttactggct atgattttag gtattacaaa ccaattcata
     2821 agaattttat atttgcattt agggtagcag gttcggcatc gtttggaaca gcaaaattga
     2881 tatattatct tggaggaatg gacaattgga ttaatttgtc ggcaaaaaat cctactttta
     2941 atcaagatat taaaattgac cctgaagtaa attacgtata tcaagctgta gcaacaaatt
     3001 tgcgtggttt taatcaaaat attaggaatg gaacaaattt tactgttgtt aatgcagaat
     3061 taagattgcc tgtagtaact tatttttaca aaaaaccttt gaataataat ttccttaaag
     3121 attttcaagt tattgcattt tttgacatag gttctgcatg gagtggtttg tcgccattta
     3181 gtggaaataa tgcctatgaa aatgattatt acaatgattt tccagtgagt gtgattgttt
     3241 ataataataa tttcccaata gtttcaggat atggatttgg attaaggtct aaattgttag
     3301 gatattttgt aagagccgac tgggcttggg gaataaatgg agacaaaata caaccaagaa
     3361 tgttttacct atcattaagc accgattttt aatgaaaaaa acgaaaaata tttcaaaaaa
     3421 aacaaatgaa aaatttaaaa gataaaatac agaaattaaa cgaaaaatat ttttccgaaa
     3481 ttgttgaatt tagaaggcat atacatcaat atcctgaatt gtcgtttaag gaatttgaaa
     3541 ctgcaaaata tataaaaagt attttaacaa aatataatat tgaatttgat gaatcttttg
     3601 gcgaaaatgc tgtgattgga attattaaag gcgatttatc tggagacaat attgctttac
     3661 gtgcagatat ggacgctttg ccaattgagg aattagtgga ttctgagttt aaatctaaag
     3721 ttcctggggt tatgcacgct tgcggacacg acgcacatgt ggcttcgctg ttggggacag
     3781 caataatttt gcaagaatta agaaattata tcaaaggaaa tattatttta attttccaac
     3841 cagctgaaga aagaaatccc ggtggtgcta aaatattgat tgataaaggt ttattgaaaa
     3901 aatacaatat tataaaagtt ttaggacaac atgttttgcc agaattagaa gttggaaaat
     3961 ttaattttgg ttcaaactat gtaatggcaa cctctgacga aatttatata aaatttagcg
     4021 gaaagggcgg acatgctgct ttgccacata agagaagcga tacagttttg gctttaattc
     4081 attttataaa cgaagtaaac gagtttaaaa tcaagttaaa gtctgatatg ccaattatta
     4141 ttgcatttgg aaaattgtct gcacaaggct ctataaatgt tgttccagag atttcttttg
     4201 cggaaggaac tatgcgaact tttgatgaaa aacttagaaa tgagattaaa gcaaaacttc
     4261 aagtaattgc agataaatat gctgattatt atggctgtaa agccgattta gaaattcgcc
     4321 atggttatcc agcggtttat aacgccccaa aattaaattc tcaagttatg gatttagcta
     4381 aagatttttt aggcgaagaa aatgttggag aattagaaat tagaatgact gctgaagatt
     4441 ttgcttttta tagtcatgaa gtgccatcgg ttttttacag agctggagtt gcaggtaatg
     4501 gaaagggtat agcaggtcag cacacttctt attttgatat agatgaaaat gtatttttaa
     4561 aatcttcagc tttaatggct tggtttactt taaatattca ataaaatgcg aaaattacca
     4621 attgaagaaa ttggcagatt aagtgccgaa gagtttgcaa aaacagaaaa actacctctt
     4681 atagtggttt tagaagatat tcgttcacat cacaatacag gagcaatttt tagaactatg
     4741 gatgcttttg caggcgaggc tgtttattta tgtggaatta caggcacacc acctcataga
     4801 gatattcata aaacagcttt aggagctaca gaaaccgtaa attggaaata ttttaattct
     4861 gctttagagg ctgtagagca attgaggcaa gaaaattata aaattatagc tttagaaatt
     4921 gccgaaggaa gtcttgatat tcaagatttg gaaatttgta aaactgaaaa aatagctctt
     4981 gtgcttggca atgaggttta tggtgttgac caaaaaattc ttgatatttg tgatttatgt
     5041 atagaaattc cacagtatgg aactaagcat tcgttaaatg tttcggtaag tggaggcatt
     5101 gcaatttggg aaatctgtaa gcgtatgtta gcgtaatttt tttattacaa aaacattgtt
     5161 cataagattt taatctaaat tcgtttattt tttggtaaga ttttacaatc tttttatctt
     5221 tgggaaaaat taaatttaat taattatgaa gtttggcgtt ttaatatttc cgggttcaaa
     5281 ttgtgaccat gatatgattt atgttttaag agatatttta aatcaagaag ttgttgagtt
     5341 gtggcataaa gatacagatt tacaagattg tgatgctata gcagttccag gtggttttac
     5401 ttatggagat tatttgcgtt caggggcttt ggctcgtttt tcgccaataa tgaaaaaagt
     5461 tgtagaattt gcacataatg gaggcttagt ttttggtgtt tgcaatggtt ttcaaatttt
     5521 atgtgaagca ggtttattgc caggcacttt attgcataat gaaaatcaaa aatttatctg
     5581 caaaaatcaa tatataaaag tagaaaacac cgatacagca atgacaatgt ttgtggataa
     5641 ggacaaagca ttaaaaattc ctatagcaca tgggcaagga agatattatg caccggattc
     5701 tactttacaa gaaatgcttc aaaacggaca aatagtttgg cgatattgtg atgaagacgg
     5761 taaattaaac caaaaagcta atccaaatgg ttctgtaatg aacattgcag gcgtttgtaa
     5821 tgttgagaaa aatgtatttg gtatgatgcc acaccccgaa agagccgccg acccagaatt
     5881 aaaaaatact cacggactaa ttatctttaa atctatgatt gagtatttta atgaccaaaa
     5941 aatgttggag aagaaggaga tgaattaggg cattaaagca actccaaaaa ctttattttt
     6001 gttttgtaaa tatatattac tgccgaattt aattaatata tggcattaag ctggaatgaa
     6061 ataaaagaca gggctttaaa gttctcaaac gaatgggctg atgcttcaag cgaagaagca
     6121 gatgcaaaac catttttagt tgagtttttt aatgtttttg gtattagcag aaaaagagtt
     6181 ggcacttttg agcatagagt aaaaaaactt gacgacagag acggctatat tgatatgcta
     6241 tggaaaggaa cgattttaat tgaaatgaaa agtcggggca aaaaccttga taaagcatat
     6301 caacaagcag tagattatac acaaggtctt aaacaatacg aattgccgaa atatattctt
     6361 gtttcagatt ttgaaaactt tagactttac gaccttgaag ataatacaga aatagaattt
     6421 aaactcaacg accttgtaaa taatgttcag aattttggct atttgcttgg ctatcaaagg
     6481 aaagtttaca aagcagaaga cccagcaaat atcgaagcag ccgagttaat gggtaaactt
     6541 cacgacagac ttaaagaaat tggttactct ggacatcctt tagaagttta tttagttcgt
     6601 ttgctttttt gcttgtttgc tgaagattct accatttttg aaaaacaaca atttcaagat
     6661 tatattgaac aacgcacaaa tattgatgga agtgaccttg cagcaaaatt gcaagaactt
     6721 tttcaagtat taaacactcc aaaagacaaa agatttaaaa accttgacga gcaacttgtt
     6781 gaatttccct atgtgaatgg gaaattgttt gaagaaatac tgccattggc aagtttcgac
     6841 agcaaaatgc gtcaagctct tttagattgt acttatattg attggagtaa aatttcgcct
     6901 gcaatttttg gttcaatgtt tcaaagtgtc atgaatccaa aggaacgtag aaatcttgga
     6961 gcacattaca caagcgaaac aaatatttta aaacttatta aaccgctttt ccttgatgaa
     7021 cttcaagcag aatttgaaaa gctaaaaggc aataaaaaca aactcaaaga atttcataaa
     7081 aaacttggaa gtctaaagtt ccttgaccca gcttgtggtt gtggaaattt cttgattatc
     7141 acttatcgag aattgcgact tttagaaatt gaagttctta aagaattgta tggtactcaa
     7201 caagtttttg atattggaca aatcatgctt gtaaatgttg accaatttta tggcattgaa
     7261 tatgaagaat ttcctgcacg aattgccgaa gttgcaatgt ggttgattga ccaccaaatg
     7321 aatatgttgg taagtaatga gtttggacaa tattatgtac gtttaccatt aatgaaatcg
     7381 gcatgtataa tggttggaaa tgctcttcaa atggattggt caacacttac taaaaataca
     7441 gaaattgata taaatgccaa tactaccaat ataattatgg taagcgaacc tgaaattcat
     7501 tacgatactg ttaatatttt tagtaaaaaa gtgcaatttt ttgatggcac ttatccaaaa
     7561 tcattttcgg gcaagcctca tttcgattat attctcggta atccgccatt tgtaggtaaa
     7621 aaagagcaaa accatgagca aaaacttgat atgcaaagag ttttttctgg agttaaaggt
     7681 tttggcgtat tggattatgt tacagcatgg tatgtaaaag ctgctcaact aatagctaac
     7741 acatcaacca aagttgcttt tgtttctacc aattccataa ctcaaggcga acaagtaggc
     7801 attttatgga atttattatt taatcattac aatattaaaa ttcattttgc tcatcgcact
     7861 tttaggtgga gtaatgaagc taaaggaaat gccgctgttc atgttgtaat aataggtttt
     7921 gcaaattacg atacaaataa taaaaagatt tttgagtacg aagatattaa tagcgaacct
     7981 cacgaaataa aagcaaaaaa tataaatccg tatttggttg aaggtcgtga tttagtagta
     8041 actaacagaa accaaccgat ttgtcatgtt cctaaaatga attatggaag catgccgatt
     8101 gataatggac acttaatttt gacaaacgaa gaaaaagaaa ttgcgataaa aaacgagcca
     8161 tttatcgatg aacttataag accctataca ggaggatatg aatttattaa caacattcag
     8221 cgttggtgct tatggttgca aaatgtaaat ccaactgtat taaaagcatc gaaatttgtt
     8281 cttgatcgaa tcgataaaac aagagaattt cgtctgcaaa gcaaacgggt tgcaacaaat
     8341 aagttagccg aaacaccaat gctttttggt gaaatcaggc aacctgatag caactatatt
     8401 atcattccaa aagtatcttc agaaaataga aaatacattc cgataggaat tctatcacca
     8461 tctattatcg ttagtggaag tgctttaatt attcctaatg ccaataatta tatttttgga
     8521 gtaatgactt cttctatgca catgcattgg atgagaacaa cttgtggaag aatgaaaagt
     8581 gattatcaat attctgccgg aattgtttac aacaatttcc cttggcccga aaatccaagc
     8641 gaaaagcaaa taaaagaaat agaagaagca gctaaaaagg ttttagatac tcgtttgcaa
     8701 tttccaaact cttgccttgc cgacctttac gaccctttaa caatgccacc tgcattggta
     8761 aaagcacata acgaattaga caaagcagtt gatttagcat atcgtccaca accatttaca
     8821 agcgaagcaa acagaatggt ctatttattt gaactttacg aaaaatatac tgtggattta
     8881 tttgcaaaag caaaaattaa aaagacaaga aaggctaaaa ataatgacta aaaacatgaa
     8941 aacatgcaat aaatgtcttc ttgacgaaaa atatcctcaa ctaacattca acgataaggg
     9001 tgtttgttct ctgtgttctt ctaacaaggt gtttaagcct attggtgaga aagctcttct
     9061 cgaaattttt gaagcagcaa aaagtaaaaa caaagaatat aatgcacttg tgcctttaag
     9121 tggaggtaag gatagtacat atattttgca tttggcagtt aatgtttata agctaaaggt
     9181 tttggcaatg acctatgata atggtttgtt tagtcagctt gctttagaca atatcgaaag
     9241 agcaataaaa attactggag taaaacatat tttcagcaag ccagactttg atgtgcaaaa
     9301 aaagatttat caaaatatgc ttaggcaaac aggggatatt tgcggagctt gcgatattgc
     9361 aacaaaaaca catactttaa aagttgcacg agatttttca attccaatta ttctatacgg
     9421 aacttcgcct ttagaaaacg actcttttat tcctgattct attcaagata ttgaaagatt
     9481 taagtatata atgaaaattt cgggtaactt gacaaaaaaa caaattaatg attttctaat
     9541 ttaccctaac ttaaatatgt tacgtcattc tatttacaaa aaaataggca tatttagtaa
     9601 agaagtaagt cctttgtttt atattgataa tcctacagac caagaaatgg gagaaatcat
     9661 aaaaaaagaa atgggctggc aagatgaaac atctcgtgaa tattctaaac acttagattg
     9721 cattgctgaa tcattaacaa actatattag aaataaaata tatggctatg aacgtagaaa
     9781 atgccagtat agcaatatga ttagacgtaa tgaaattaca cgcgacgaag ctatgcgttt
     9841 gtttaatgca gataaaattg aggaaaaacc atctaattat tcaactgttt taaatcatct
     9901 tgaacttact gaagatgaca tcaataaggc aatttcatat aaacctctga cttttgaaaa
     9961 tcgaacttca aagatgaata ggctccagac atatctgatg aagaaaataa ggaataaagg
    10021 ctaagtgcag tatgaattgt tttggaatta acagattgtt cacaatcata tcatttgttt
    10081 tcacaacact gtcagtcttc tctcagacag caaaaacgcc gcttatgggt tggtcaagct
    10141 ggaataatta tggacataat attaacgatt ctataattaa agctcaagct gatgcaatgg
    10201 tgtattctgg cttagcggat cttggtttta aatatataaa tattgatgat ggctttttcg
    10261 atggtcgtaa tgctgatggt tctttgcgta ttaatgcaac aaaatttcct tatggaatga
    10321 aaactctagc tgattatatc cattctaaag gattaaaagc aggattttac tcagaagcag
    10381 gagcaaatac ttgtggctct atatggggag ctcaaactgg tggagtagac ggaggtttgt
    10441 acaatcacga ccaacaagat atagattcaa tttttaaatc ttggggattt gattttttaa
    10501 aagtagattt ctgtggtgga cagcaacaag gtttagacga agaaactcgt tatacacaaa
    10561 taaaaacagc tataaacaat acaggtagaa cagatataaa ttttaatgta tgtcgctggc
    10621 aatttcccgg agcatgggtg tgctcagtag cagattcttg gcgtatgtca ggcgatataa
    10681 atcttacacc aggctcaatt ccaagttgga acgaaatact taaaattatt gatcaaaata
    10741 cctttttagc cgcatatgca agtcccggtc attacaacga tatggatatg cttgaagttg
    10801 gcagaggact aagtttcgaa gaagataaaa gccatttcag catatggtgt attttatcat
    10861 cacctttggt tttgggcaac gatttagcca atatgagtga gcaaacaaag agtattttga
    10921 caaatcccga agttattgct gtaaatcagg acactactgg actgcaagct cgccgaattt
    10981 gggataatgg ctcaggatta caagtttggg caaagaattt gaatggaaaa ctaagcaatg
    11041 aacgtgcagt tgttttgttc aataggagag acacggcagc ttctatatct gtaaaatgga
    11101 aagatttgaa tttggaaggt ccagcaacag ttcgtgatct ttgggaaaga agagatttac
    11161 gaataatgga ttctatgttt acagccgatg taccgtctca tggtgtagta atgctgaaag
    11221 ttgtaggaaa acaaaacaaa ctacagcaag tctttgaagc agagtacgca tggattaaca
    11281 cgtttaattt aactaaaaat agtaaaatac ttccaaatca gggcagggca gtggttgata
    11341 ctctatgttc gggacttgca aaagttgctt ggcttggcaa tagtgccgat aattatatag
    11401 aatttcgcga tgtttatgtt tactctgacg gaaaatacaa gctaagcatt tcatacatta
    11461 gcggagaaaa tagaaatata actttaagtg taaatggaat tgacaaagtt ctaaaaaaac
    11521 taaattctgg agattggaaa actattgata ataaaacaat tactgttaaa ctaaataagg
    11581 gttacaatac aattcgactt tctaatgcaa atgggtggat gccagatgtt gataagattc
    11641 agttaaaagt taaaagtcga aagtcgaaag tcgaaccgag cctgcgagct caccgaaggt
    11701 aaagaggctg acgcgtaatc agttaaaagt aaaaaaataa ttgataattg aataattcag
    11761 cttcgctgca ttttctgtcg tctctcggta tagttcaagc aagcttggct ctacactcgc
    11821 tccttagaaa attgataatt aagaattcaa gtttgactct gcattcgctc cttagaaatt
    11881 tataattaag aatcagttaa aattgatata ttaaacataa ttgaagtgtt aatagaggat
    11941 ttttttattt cagatttaat ttagttaaaa gtttttttta tttttgtttt ctaattatat
    12001 gtttttcaaa ttttaaagaa cgatgtttaa aaattatcct aaaacaagaa tagagcttcc
    12061 cgaaaagttg gaagcaattt ataatcagca ttataaatca aatagagatg gagacacaac
    12121 agcttctggc ttagctcaaa agatggaagc atggttgcat aaaaaagttg cagcagatgt
    12181 aaaaggctct catgataaaa aaacattaga aataggagca ggcacattaa atcaattaaa
    12241 atatgaacag tcaagtcatt atgatattgt tgaacctttt agtgaattgt ataaaaattc
    12301 tccatatatc acacaaataa aaaatattta taatgatatt gatgaaattg acatctcaca
    12361 aaaatatgat agaataactt ccgtagcaac ttttgaacat attttagatt taccaaaagt
    12421 tgtagcaaaa acttgtcttc ttttaaatcc taacggctca cttagaacaa gtattccaaa
    12481 cgaaggcact tttttatgga ctttaggctg gaaacttaca actggattag aatttaaaat
    12541 taaatatggt ttggattatg gaattctgat gaaacatgaa catgtgaata aagctgacga
    12601 aatagaagaa gttttaaatt atttttacaa aaaaaataaa tgctatacct ttggactatg
    12661 taaaaaagtg gctttttata gattttatga aagcaaagaa ccaaatattg agttagcaca
    12721 aaaatatctt gaaagtttgg ataaataatt actgacaggc aagatttgtg catattctca
    12781 attattctaa aatacatagt tagctactca ggaaaattga taataattta aaacttgaaa
    12841 atttttaaca tataagcaaa aaaaagaggg ctaaaccctc ttttccatag ttttttttac
    12901 aaattaatac tcatcttcat taaagaagaa atcatcttta cttggataat cgggccaaat
    12961 atcctctata ctttcgtaaa tatcaccttc gtcttcaatt tcttgaagat tttcaataac
    13021 ttccaaaggt gctcccgaac gaattgcata atcaataagt tcgtcttttg ttgctggcca
    13081 aggggcatct tctaatttag aagctaattc aagtgtccaa tacatattaa tatattttag
    13141 ttttttattg gttcaacatc tatctaaaaa atggattgcg aagctataac aatttttatt
    13201 aataaccaaa cattatttcc acaaaatttc atcaatgtta taaacaatag catattttct
    13261 gcctaaaaca aacaaataat cagataatct gttgataaaa ataaaaattt catcaggaat
    13321 aaaaatttca tcttttgcag aaacaataag cctttcagca cgtctgcaaa cagttctaga
    13381 aatattacac caactaataa attcgcttcc agctggtagt acaaaacttt ttaattttgg
    13441 taaattttca ctgtttttat ctatagcttg ttctaaagta gaaatatctt catttgttat
    13501 gtgccttaat tgcggtagct tatatttagc atttttttcg tcagcagcta aaattgagcc
    13561 aatgtcgaac aagcgatttt gaacaaaatt tataatctct ttatcagaat tatcaatttt
    13621 aaaatttaga agcattccaa taaatgagtt taactcgtca attgtgccat aagcttctat
    13681 gcgtaaacta tcttttctta caatttgacc acctaaaagt gatgtgtttc ctttatcgcc
    13741 aacttttgta tatattttca tgacataaat ttttaatatt aacttaaatc cattgttacg
    13801 tttttgttta tttcatcaga ttctattaaa ccatctctca atcttactat tcttcgtgca
    13861 tacaaagcaa tatcttcttc gtgtgttact aaaataattg tatttccttt tttataaatt
    13921 tcttcaaata aagtcattat ctcaattgaa gttttggtgt caagatttcc tgtaggctcg
    13981 tcagcaagaa tgatagaagg cttgtttacc aatgcgcgtg ccacagcaac tctttgacgt
    14041 tgtccaccag aaagttcatt tggtttgtga tgcgctctat ctgttaatcc aacagattca
    14101 ataacttctt gggctcgttt ttgtctttct tttgaagaaa cgcctgcata aactaaaggc
    14161 aaaacaacat tttcaaaagc agtatagcga ggtaacaaat taaaagtttg aaaaacaaaa
    14221 cctatttcct tattcctaat ttctgcaagt tcgttgtcgc tcataaaact cacatcagta
    14281 ccgtttagtt catattgacc tgaagttaaa c
//
LOCUS       DAOPNX010000069        13457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_5342_length_13457_cov_7.073944, whole genome shotgun sequence.
ACCESSION   DAOPNX010000069 DAOPNX010000000
VERSION     DAOPNX010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13457
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5342_length_13457_cov_7.073944"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..2076)
                     /locus_tag="PKN53_08100"
     CDS             complement(181..2076)
                     /locus_tag="PKN53_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOD88947.1"
                     /translation="MKNIFLSFIVISVLFSNVLSAQTEIEISTEKTIINNEKFYIHTV
                     AEGNTIYSICKAYGVSEEQLYKANPDLEGTNIKIGQTLQIPVLNEFSADGKHIIYTVK
                     PGDTLYSLCRKYGVSEVDFYTINPDIKQGKPLKVGQEIMFPYNLIDTKIQETDKDKDT
                     VNFIYHLIEKGETIYGISRIYNITKEELINLNPEIDNNRLLVGNVLKIPKKNEVEKNI
                     PEKNESTNKNNLDNNANDIEDIDNFEDLSNIDFDNDKNWYSFENKVEISILLPFEVDA
                     NLNNLYRQETGKKEQRLYLLTEQIISFYSGILLALEKFENNNVDISVNVYDIGKDNTV
                     ISNLLKDGKLKSTDIIIGPAFKSQVDYLNKNLYDTKPYILLPFVNHSEVVINNNKNIL
                     IRPTAEAVIDCLASYAAENKDNLYLIIQGTTAEQIKLANEHYDAMVSKLGSSTNVKII
                     KYSGGKLLNLESSVSKTQENVFILPFQNEVSCTKIFLDLFPLKDYAITLIGNPAILEY
                     ETIDPKYYLSSKFTYFSGTNIDYTDEETKTFVESYRKMFLIEPDENSFLAYDAINFWV
                     RNYLKYGNSLARMVANENEHKGLSGKQVFVAKPQYSAQSYSNSNVYLFQLQEDYSFEQ
                     IFSESKD"
     gene            complement(2263..3723)
                     /locus_tag="PKN53_08105"
     CDS             complement(2263..3723)
                     /locus_tag="PKN53_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOD88948.1"
                     /translation="MSEIKKLNPERVWKHFYSLTQIPRPSKKEEKVIEFMKNFGESLG
                     LETIVDHIGNVIIRKPASPGMENRKGVVLQGHLDMVPQKNSDKVHDFEKDPIETMIDG
                     EWVKANGTTLGADNGMGCAVAMAVLEDKSLVHGPIEALFTIDEETGMTGANHLKPGLL
                     KGDILLNLDSEDEGELYVGCAGGVDVNVTMNYPEVDMPKGFVAYKLSVTGLKGGHSGM
                     DINIGRANSNKILFRFLTYATHFYDLRIAEINGGSLRNAIPRESFAIVAISEKEASKF
                     EGEVAKFEKIFKQEFSKSEPDLQMKVEKTNAPAKIMANDEMHKICKAVYACPHGVQRM
                     SDAMPGLVETSNNLAIVKCINGKFAVHCLVRSSVDTAKEGVMYKIGGLFNLIGAEVAY
                     EGAYPGWKPNMDSPILKTMLKTYEKLYNKTPEIKAIHAGLECGILGAAYPNWDMISFG
                     PTIRYPHSPDERILISSVEKFWNYMVETLKNIPVKE"
     gene            3822..4823
                     /locus_tag="PKN53_08110"
     CDS             3822..4823
                     /locus_tag="PKN53_08110"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HOD88949.1"
                     /translation="MKKIFLTLTLILFVQLIFAQTTINWLDFETAIEMQKENPKTIII
                     DMYTDWCGWCKKMDAETFQNPHIANYINTFFYPVKFNAEGFDSINYKGKVYVNPNVGR
                     RSTHQLAQELMGGRMSYPTMVYIDFEEQVFPVPGYMNPQNLEPLLIYFAERINKLANF
                     VDFQREFKETFDPDSTAKNTGDINWVDLEQALELRLTKPKKILLFVNSDFNNSSKVML
                     ASSLRHPVISNLINENFYPVKFNFNHQDTVQVYENTFINEQLQPNYPHQFIIALLQPD
                     IRLPSIVFFGEDFSLIFALRGYQSAQQLERFLEFFSKDLHKTGEDWTKFNENFKSKL"
     gene            4877..6952
                     /locus_tag="PKN53_08115"
     CDS             4877..6952
                     /locus_tag="PKN53_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOD88950.1"
                     /translation="MKKLIWLILLSGFLISKTYSQKCSIDEAENIAINTINEKINPIS
                     KNAQSFEIKNIEVVYFNENEVFYLVNLRPQGFILISSEKSVEPVLAFSLESDYELENN
                     PSAKFWINNYAKQIEYSIQHVTKATLNVDKEWQRLSRDPKDFIPKSNIKTVEPLLFTK
                     WSQGKYYNAHCPVDAAGSDGHVVVGCVATAIGQLMNYFRYPTVGMGSYGYEHQTYGWL
                     EVDFSEQHYDYDLMPVFPTDYNDNLARLLYNIGVSVDMNYGPQGSGMYNHKGAYTLYT
                     YFDYAPETTYLFRDSLPEDFDWNGNLVFHLDQKIPLYYAGWGDYDYISGHAFILDGYS
                     DSTHYHINWGWGGSMDGYFFIENLKPGGSDFTLAHEVIANAVPNNPCQGCSELKILNS
                     IEGVIEDGSGPLNNYQNDLECSWLITHNDSITGVEFEFLKFDLDENDYLIFYEGENEQ
                     APVANTFFGGQTVENFTVNSDKVLVKFVSDAENTNLGWLLKYKAVLPKYCDIIKVLTE
                     PEGIISDGSNSYQYHNDTYCTWRIQPADAENIEITFLEFDTEPINDYVRVLNSSGQAV
                     GTFSGDALPDKILIEGNKATVIFKTNNDIRAGGFKLKYQTNVNSSEEIFANKIEIYPN
                     PAEDFLNVKINNDRNYTINIIGIDGKIYLNKTENSNSQINIENLSSGIYFIEIIQDGE
                     ILREKFVRK"
     gene            6980..7168
                     /locus_tag="PKN53_08120"
     CDS             6980..7168
                     /locus_tag="PKN53_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88951.1"
                     /translation="MACRINVKGGNMKRFTIIIFLLALYINAFSQDEDLQMIFDSIIA
                     EADLMYKYEKLFGIQQIC"
     gene            7168..7809
                     /locus_tag="PKN53_08125"
     CDS             7168..7809
                     /locus_tag="PKN53_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88952.1"
                     /translation="MINKNLQKNYGGYVVSHSADSVYVVYIDKKQEQSIARYTYTSAD
                     LSKPCYISTELSTLSVEEKELVDIKTKMLKQIVNRKYKVTIPNGFSPNFVLIKEDNFY
                     KLYVIMGTSTYGVIPFGNDYLFKANSKGKIIYWTKFHSTMIPVMAETDGHKVVEATHS
                     HLRTTPYITATDICTFRLYAQYTELQKFSVYSPAIKKTMTYILETNKIEVKEK"
     gene            complement(7865..9031)
                     /locus_tag="PKN53_08130"
     CDS             complement(7865..9031)
                     /locus_tag="PKN53_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD88953.1"
                     /translation="MSKEFIKRDITDVMLEMYKYFPVLTMTGPRQSGKTTLLRKVFDH
                     LPYYSLENLDIRNFALNDPVGFLSQHQEGMILDEVQNAPDLLSYIQGLVDENPEKRFV
                     LSGSSQFSVIKKITQSLAGRTGILELMPLSYNEVKTQADKKNLDEILITGFYPVLYSG
                     KNIANLFYPSYVKTYLERDVRDLLQIKDIMQFQTFLRLCAGRIGSLFNASELSSEVGV
                     SVNTIKSWLSVLQASYIIKMLAPFYENMKKRLTKTPKLYFCDTGLACYLLGIETEQQL
                     ARDKMRGHLFENFIVMEAFKNRHNQGKDSNLFFYRDSNGVEVDLLLKNGNNYSAIEIK
                     SSQTYHIEFETGIKSLESLLKDRLSNKAILYAGDFENDTAQIQVLNYKNMGRVV"
     gene            9234..9569
                     /locus_tag="PKN53_08135"
     CDS             9234..9569
                     /locus_tag="PKN53_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88954.1"
                     /translation="MGKTLIWGILLTTIVFLISTFVGARLNLNIAFFNASFGTDMLML
                     ILSIGLIYGLKKYVRYRIALPRFRKMLKPMLFGFLAAVIINSLLSGIGNIFKIESESH
                     FAFDIMSPL"
     gene            9582..9929
                     /locus_tag="PKN53_08140"
     CDS             9582..9929
                     /locus_tag="PKN53_08140"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOD88955.1"
                     /translation="MFILASIAEEVLFRGFLQNILKPLQNKGIKIFKRHISLPVIVAA
                     LAFSLVHLILITSGAGAYFIFRTLVFAFVLGLIAGYYQEKHNNNAYAIIVHMSGNLMG
                     VIASLIVSFGNLS"
     gene            9941..11077
                     /locus_tag="PKN53_08145"
     CDS             9941..11077
                     /locus_tag="PKN53_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOD88956.1"
                     /translation="MKFKFVIIIGIVVLIVAIAIAIFAYINMNYDYLNEHFLEKKGYK
                     LGFTENTAKLNDGSEIYYIEGPNNGPKLLLLHGQQVSCYDYAKVLPNLSKEFHVFALD
                     YYGHGKSSKNPDKYNAVEIGNDIIWFVKNVIKEKIYISGHSSGALLAAYVSANAPDCI
                     IATILEDGPFFSTLPNRAEKTISWLEFKNMYDYLNQNEIGSFMEYSLEHNYMQEIFNA
                     KDPQAWNKVIKNPALKYLEKHPGEIPIIWYYPPKLGINTIYALNANMQDGTGKYDLRF
                     GLTFYDFSWFEGFDTEEILKNIKSPTIVMHVAPSDITAPSYYDENGVLLAAMDKKDAQ
                     KVVDLVPNSKYAGGFKSAHDIHADLPDEFIEVLLDLKNQIEKTN"
     gene            11085..11843
                     /locus_tag="PKN53_08150"
     CDS             11085..11843
                     /locus_tag="PKN53_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88957.1"
                     /translation="MKRVFIKDKTKFIKRNKAHKIEMFSEELIKDLPEPLKKYLRVCG
                     YINTPVPVNANIYWTESWLKMSPAKEWSELHTTQFNSVKPIGRLAYMKILDMPVAARD
                     LYRDGYGEMKVKLFNLINLALDNSKEVAQSALITIFCEFAFIPSYLLSENVKWEQIDD
                     YSVRGTLVDNGIEVSGMFYFNEEGLFTHFETFDRYFTTGKNSYKKVKFSVVVESFKTQ
                     DNLKIGEKVKIIWHLPESDYEYYKGTVDRIEFNV"
     gene            12761..13261
                     /locus_tag="PKN53_08155"
     CDS             12761..13261
                     /locus_tag="PKN53_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88958.1"
                     /translation="MILAIIFSTILSGAFLKVIELKYSWILILKYILLFFPMSLGIGL
                     FAFLLIPNTIQNWKNSKTKSVLLIFSISIFFFIAFYVDTLFQDIELAATMAFIGLLLG
                     LAYLFLRNFWLVYSTLFLIMLVNTLADNKYDEYNFWIVIISTILSLTILTFDFIKNRK
                     LKNTNL"
     gene            13272..13451
                     /locus_tag="PKN53_08160"
     CDS             13272..13451
                     /locus_tag="PKN53_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88959.1"
                     /translation="MTTEKAKSLQAILDKKVDGKKIFGTSFALKKDNTVWQGASGNLS
                     RDIQKLLIKRQQIDC"
BASE COUNT         4712 a         1935 c         2066 g         4744 t
ORIGIN      
        1 cgctgattaa caacggatta gctttggtgt cccaaaaaag tgcggaactc aggaacttaa
       61 tattttaacg gattttttac aaaaaggttg ctaatttgta actaaattaa taaaatcgtt
      121 attaaatagt tgttcttcta aattgctgtg ttaaaaatat aaagaaagtt tgagtttaat
      181 ttagtcttta ctttctgaga aaatttgctc aaaagaatag tcttcttgta attgaaacaa
      241 atatacattg gagttggagt aggattgtgc agaatattga ggttttgcaa caaaaacttg
      301 ttttccacta agtcctttat gctcattttc gtttgctacc attcttgcta aactatttcc
      361 atattttaag taatttctta cccaaaaatt gatggcatca taagctaaga aagaattttc
      421 gtcgggttca attaaaaaca ttttgcgata gctttcgaca aatgtttttg tttcttcatc
      481 agtataatct atattagttc ctgaaaaata agtgaactta gaacttaaat aatatttagg
      541 gtcaattgtt tcgtattcta aaatagcagg attgccgatt aaagtaattg cataatcttt
      601 tagtggaaat aagtctaaaa agatttttgt gcaactaact tcgttttgga aaggtaaaat
      661 aaaaacgttt tcttgtgttt tagaaactga actttctaaa ttaagtagtt ttccgccact
      721 atatttaatg attttaacat ttgtagaact acctagtttt gaaaccattg catcataatg
      781 ctcatttgct aattttattt gctctgctgt tgttccttga attattaaat ataaattatc
      841 tttgttttct gcagcatatg aagcaaggca atctattaca gcttcagctg taggtcgaat
      901 aagtatgttt ttgttgttat taattactac ttcactatga tttacaaaag gcaataaaat
      961 atagggtttt gtatcgtaaa gatttttgtt taaatagtca acttgagatt taaaagcagg
     1021 accaattatt atgtctgttg attttaattt tccgtctttt aacaagtttg aaattacagt
     1081 attgtctttc ccaatatcgt aaacatttac agagatatct acattgttat tttcaaactt
     1141 ttctaaggca agcaaaatcc ccgaataaaa cgaaattatt tgttctgtaa gcaaatatag
     1201 tctctgttct tttttgcctg tctcttgtct gtataagtta ttcaaatttg catcaacctc
     1261 gaaaggtaac aaaatcgaaa tttcaacctt gttttcaaaa gaataccaat ttttgtcatt
     1321 atcgaaatca atgtttgata aatcttcaaa gttgtcaatg tcttcaatat cattagcgtt
     1381 gttatctaaa ttgtttttat ttgtcgattc atttttttca ggaatattct tttctacttc
     1441 atttttttta ggaattttta aaacatttcc taccagaagt ctattgttgt caatttcagg
     1501 atttaagtta atcaactctt cttttgtaat attatatatt cgtgatatac catatatagt
     1561 ttcacctttt tctattaagt gataaataaa attaacagta tctttatctt tatctgtttc
     1621 ttgaatttta gtgtcaatta aattataagg aaacataatc tcttgaccaa cttttaatgg
     1681 ttttccttgt tttatatctg gattgatggt gtaaaaatct acttcactta ctccatattt
     1741 tctgcataat gaatataatg tgtcgccggg ttttacggta taaatgatgt gtttcccatc
     1801 agccgaaaat tcattaagta caggtatttg taaagtttgt cctattttaa tatttgtgcc
     1861 ttctaagtct ggatttgctt tataaagttg ttcttcgctc acaccataag ctttacaaat
     1921 tgaataaatg gtgtttcctt cggctacagt gtgaatataa aatttttcat tattgattat
     1981 tgttttttca gtagatattt caatctcagt ttgtgctgat agaacattcg agaataaaac
     2041 agaaattaca ataaacgaca aaaaaatatt tttcataata caaattttga cacaacaaaa
     2101 ttacaaaata tttttagaaa acaaagtagt atctctaaaa gaaattttat tgtttaataa
     2161 taactatttt aaggcaaaat ttagaccaaa accaaataag ttgtcttaaa attgttcaac
     2221 cttaagctaa gattgtaaca attttaagac aatataaaat ttttattctt ttacaggaat
     2281 attttttaga gtttcaacca tatagttcca gaatttttca actgaagaaa ttaatattct
     2341 ttcgtcagga gaatgtggat agcgtattgt aggaccaaaa gaaatcatat cccaatttgg
     2401 ataagcagcg cctaaaattc cacattctaa accagcgtga atagctttta tttctggagt
     2461 tttgttgtat agcttttcat aagttttaag catagttttt aaaattggag aatccatatt
     2521 tggtttccag ccaggataag ctccttcgta tgcaacttcg gcacctatta agttaaataa
     2581 gcctccaatt ttgtacataa cgccttcttt tgcagtatca actgaactcc ttaccaaaca
     2641 atgtacagca aattttccat ttatacattt tactattgct aaattatttg atgtttcaac
     2701 caagcctggc atagcatcgc tcattctttg aacgccgtga ggacaagcat aaactgcttt
     2761 gcaaatcttg tgcatttcat cgttagccat aatttttgct ggagcattgg ttttttcaac
     2821 tttcatttgc aagtctggtt cagatttaga aaactcttgt ttaaaaattt tctcaaattt
     2881 agcaacttcg ccttcaaatt tagaagcttc tttttcagaa atagctacaa ttgcaaacga
     2941 ctcacgagga atagcatttc ttaaacttcc accattaatt tctgcaattc ttaaatcgta
     3001 aaaatgtgta gcataagtta agaaacggaa taatattttg tttgagtttg ctctaccaat
     3061 attaatatcc attccactat gtccgccttt tagccctgta acagaaagtt tgtaagcaac
     3121 aaaacctttt ggcatgtcaa cttcgggata attcatagtt acgttaacat caacacctcc
     3181 agcacaacca acataaagtt cgccttcgtc ttctgaatct aaattcaata aaatatcacc
     3241 tttcaaaaga cctggtttta ggtgattcgc tccagtcatt ccagtttctt catcaattgt
     3301 aaacaaagct tctataggac catgtactaa acttttgtct tctaaaacag ccatagcaac
     3361 tgcacaaccc atgccattgt ctgctcccaa agtagtgcca tttgctttaa cccattcgcc
     3421 gtcaatcata gtttcaattg ggtctttttc aaaatcgtga actttgtcgg aattcttttg
     3481 aggaaccatg tctaaatgac cttgcaaaac aacacctttt ctattttcca taccaggact
     3541 agcaggtttg cggataatta cattaccaat gtggtcaaca attgtctcta atccaaggct
     3601 ttcgccaaag ttcttcataa attcaattac tttctcctct tttttagaag gacgaggtat
     3661 ttgagttaat gaataaaaat gtttccaaac tctttcggga tttaattttt taatttcgct
     3721 cataatttaa ttttttaaat ttttttacta tttttgaagt cataaaacta cataaaataa
     3781 ataatattta caaaaatttg tattaaaata aatttaatta aatgaaaaaa atatttttaa
     3841 cacttacatt aatcttattt gtacagctta tttttgctca aactactatc aattggttag
     3901 attttgaaac agctattgaa atgcaaaaag aaaatccaaa aacaattatt attgatatgt
     3961 acaccgactg gtgcgggtgg tgcaaaaaaa tggatgcaga aacttttcaa aatccgcata
     4021 ttgcgaatta tataaacacg tttttttatc ctgtaaaatt taatgccgaa ggttttgatt
     4081 ctataaatta caaaggtaaa gtttatgtaa atcctaatgt tggaagacgt tcaactcatc
     4141 aattagctca agaacttatg ggtggacgaa tgtcgtatcc tacaatggtt tatatagatt
     4201 ttgaagaaca agtttttcca gttccaggat atatgaatcc acaaaaccta gaaccattat
     4261 taatttattt tgcagaaaga ataaataaat tggcaaattt cgtagatttt caacgagaat
     4321 ttaaagaaac ttttgatcca gatagcacag ctaagaacac aggagatata aattgggtag
     4381 atttagaaca agctttggaa ttaagactta caaaacctaa aaaaattctt ctttttgtaa
     4441 attcagattt taataatagc agtaaagtaa tgcttgcttc ttctttacga catcctgtaa
     4501 tttcaaactt aataaatgaa aatttttatc ctgtaaaatt taattttaat catcaagata
     4561 cagtacaagt ttatgaaaat acttttataa acgagcagtt gcaacctaac tatccgcatc
     4621 aatttattat tgcacttttg caacccgaca taaggctacc ctcaatagtg ttttttggcg
     4681 aagatttttc tctaattttt gctttaagag gatatcagtc tgcacaacaa ttagaaagat
     4741 tcctagagtt ctttagcaaa gatttacata aaacaggcga agattggaca aaatttaacg
     4801 aaaactttaa atctaaactt tagcttttga ttaagatgtt aaaaaatatt ttttaatttt
     4861 acaaaaaact gtaagtatga aaaaattaat atggttaatt ttattgagtg gatttttaat
     4921 ttcaaaaacc tattcacaaa aatgctctat tgatgaggca gaaaatattg caataaacac
     4981 aattaacgaa aaaatcaatc ctatttcaaa aaatgctcaa agttttgaaa ttaaaaatat
     5041 tgaagtagtt tactttaatg aaaatgaggt gttttacctt gtgaatttaa gacctcaagg
     5101 ttttatttta atttcttctg aaaaatcagt tgagcctgtt ttagcatttt cattagaatc
     5161 tgattatgaa ctagaaaata atccgtctgc aaaattttgg ataaataatt atgcaaagca
     5221 gatagaatat agtattcaac atgtaacaaa agctacttta aatgttgata aagaatggca
     5281 aagacttagt cgagacccaa aagattttat tccaaaatca aatataaaaa cagttgagcc
     5341 tttgttgttt acaaaatgga gtcaaggaaa gtattacaat gctcactgtc ctgttgatgc
     5401 cgctggatct gacggacatg tggttgttgg ttgtgttgct actgctattg gacaactaat
     5461 gaattatttt agatatccaa ctgttggcat gggaagttat ggatatgagc atcaaacata
     5521 tggctggctt gaagttgatt tttcggaaca gcactacgac tacgacttaa tgccagtatt
     5581 tccaacagat tacaatgata atttagctcg tcttttatat aacataggtg tgtcagttga
     5641 tatgaattac ggaccacaag gaagtggaat gtacaatcac aaaggagcat atacattata
     5701 tacttatttt gattatgcac cagaaacaac ttatttattt cgcgattctt tgcccgaaga
     5761 ttttgattgg aatggtaatt tagtttttca tttagatcaa aaaattccgc tatattatgc
     5821 aggctgggga gattatgatt atatttcagg tcatgctttt attcttgacg gatatagcga
     5881 ctcaactcat tatcacataa attggggctg gggaggctct atggacggtt atttctttat
     5941 tgaaaattta aaaccaggtg gaagcgattt tacacttgca cacgaagtta ttgcgaatgc
     6001 ggttcctaac aatccatgcc aaggctgttc agaattaaaa atattaaatt caattgaagg
     6061 ggttattgaa gatggcagtg gacctttaaa taattatcaa aatgatttag agtgttcgtg
     6121 gctgattact cacaatgatt caataactgg agttgagttt gagtttttaa aatttgattt
     6181 agacgaaaat gattatttaa tattttacga aggcgaaaat gaacaagctc ctgtggcaaa
     6241 tacttttttt ggtggacaaa cagtagaaaa ttttaccgta aattcagaca aggtattagt
     6301 taaatttgtt agcgatgccg aaaataccaa tttgggttgg cttttaaaat acaaagcagt
     6361 tttacctaaa tattgcgaca taataaaagt gttaacagaa ccagaaggga ttataagtga
     6421 tggaagtaat tcttatcaat atcataacga tacttattgc acttggagaa tacaaccagc
     6481 agatgctgag aatattgaaa taactttttt agagtttgat acagagccaa taaatgatta
     6541 tgtgagagtg cttaactctt caggacaagc tgtaggaaca ttttcaggcg atgctttacc
     6601 cgacaaaata ctaatagaag gaaataaagc tacagtgatt tttaaaacaa acaatgatat
     6661 tagagccggt ggttttaaac ttaaatatca aactaatgta aatagtagtg aagaaatttt
     6721 tgcaaataaa atagaaatat atccaaatcc agcagaagat tttctgaatg ttaaaataaa
     6781 taatgataga aattatacca ttaacattat aggtatagac ggtaaaattt atttaaataa
     6841 aaccgaaaac tcaaactcac aaataaatat agaaaatctt agttcaggaa tttattttat
     6901 tgagataata caagatggtg aaattttaag ggaaaaattt gtgaggaaat agtcgggaag
     6961 gtttaataaa gttgtatatt tggcttgtag aataaatgtt aaaggaggaa atatgaaacg
     7021 ttttactata ataatttttt tgttagcatt atatattaat gctttttcgc aagatgagga
     7081 tttgcagatg atatttgatt caataattgc agaagcagat ttaatgtaca aatacgaaaa
     7141 actgtttgga attcaacaga tttgttaatg attaacaaaa acttacaaaa gaattatggt
     7201 ggctatgttg taagtcattc agcagattct gtctatgtag tctatataga caaaaaacag
     7261 gaacaaagta ttgctagata tacttatact tctgcggatt taagtaaacc ttgttacatt
     7321 agtacagaac tttcaacttt gtctgtcgaa gaaaaagagc tagttgatat taaaactaaa
     7381 atgcttaaac aaattgttaa tagaaaatat aaagtaacaa taccaaacgg atttagtcca
     7441 aattttgttt taattaaaga ggacaatttt tataaacttt atgtgataat gggaacatca
     7501 acatatgggg taataccttt tggtaatgat tatttattta aagcaaactc aaaaggaaag
     7561 attatatatt ggactaagtt ccactctact atgattccgg taatggcaga aactgatgga
     7621 cacaaagtag tagaagcaac acattctcat ttgcgaacaa caccatatat tacagcaaca
     7681 gatatatgta cttttaggct ttatgctcaa tacacagaat tacaaaaatt ttcagtatat
     7741 tcacctgcta taaaaaaaac aatgacatat attttagaaa ccaataaaat tgaagtaaaa
     7801 gaaaaataat gaaactatct aacttaacca cattttttag taatttgtaa caagctattt
     7861 acctctaaac cactctcccc atattcttat aatttaaaac ttgaatttgt gctgtatcat
     7921 tttcaaaatc accagcatat aagatggctt tgttgcttag tctgtctttt aataaggatt
     7981 ctaatgattt tatacctgtc tcaaattcaa tatgataggt ttgtgatgac tttatttcaa
     8041 tagcagaata attattgccg tttttgagta gcaaatcaac ttcaactcca ttactatcac
     8101 gatagaaaaa taaattactg tcttttcctt gattgtgtcg atttttaaaa gcttccatca
     8161 caataaaatt ttcaaaaaga tgtccacgca ttttatctct tgctaattgc tgttctgttt
     8221 caatacctaa cagataacag gctaatcctg tgtcgcagaa ataaagtttt ggcgttttgg
     8281 tcaatctttt tttcatattt tcataaaaag gagctagcat tttgataata taagaagctt
     8341 gcaaaacaga aagccaagat ttaatagtat ttacagaaac tccaacttcg ctagatagtt
     8401 ccgaagcatt gaataaactt cctatacgtc cagcacaaag tctcagaaat gtttgaaact
     8461 gcattatgtc ttttatttgt aataaatcgc gaacatctct ttctaagtaa gtttttacat
     8521 aagatggata aaacaaattg gctatgtttt tcccagaata tagtacagga taaaagcctg
     8581 ttattaatat ttcatctaag ttttttttat ctgcttgcgt cttaacttcg ttgtatgaga
     8641 gaggcataag ttctaaaatc cctgttcgtc ctgcaagaga ttgcgtaatc tttttgataa
     8701 cagaaaattg agaactgccc gaaagtacaa aacgtttttc aggattttca tctactaaac
     8761 cttgtatgta ggaaagtaaa tctggagcat tttgaacttc atctaatatc attccttctt
     8821 gatgctgact gagaaatcca acgggatcat ttaaggcaaa attacgaata tctaaatttt
     8881 ctaacgaata gtagggtaaa tgatcaaata ctttacgcaa taaggtggtt tttccagatt
     8941 gacgaggtcc tgtcatagta agaactggaa agtacttgta catttcaagc atcacatcag
     9001 ttatatctct tttaataaat tctttactca tatttattta tgcaaatttg aagttcaact
     9061 gcaaaattac ataaaactat tcatttatcc aaattcttta ctcaaaataa aaaattgata
     9121 ataaacttgg ttgtatatat tggtaacaaa ggaaaacctt gcaaattttt tattactaaa
     9181 ataatcttta tctttgcaat ataatttatt aacgtataga tttttatatt tttatgggaa
     9241 aaacacttat ttggggaata ttgttgacaa caatagtatt tctaatatca acatttgttg
     9301 gtgcaagatt aaatttgaac atcgcatttt ttaatgcttc ttttggaaca gatatgctta
     9361 tgctaatact ttcaataggc ttgatttatg gtttgaaaaa gtatgtgaga tatagaatag
     9421 cactgccaag atttagaaag atgttaaaac caatgttgtt tggttttttg gcggcagtaa
     9481 taataaattc tcttttaagt ggtataggaa atatctttaa aattgagtca gaatcgcatt
     9541 ttgcttttga tataatgtcg ccattataga tttttcttta tatatttatc ttggcaagta
     9601 tagcagaaga agttttgttt agaggtttcc ttcaaaatat tttaaaacca ctccaaaata
     9661 aaggaataaa aatatttaaa agacatatta gtcttcctgt aattgttgca gcacttgcat
     9721 tctcattagt tcatttgatt ttaataactt caggagcagg tgcatatttt atttttagaa
     9781 cccttgtttt tgcttttgtt cttggtttga tagctggtta ttatcaagaa aaacataata
     9841 ataatgctta tgcaatcatt gtgcatatgt cagggaattt aatgggagta atagcatctt
     9901 taatagtgtc atttgggaat ttaagttaaa ataaaagact atgaaattta agtttgttat
     9961 aataatcgga atagttgtac taatagttgc aatagctatc gcgatttttg catatataaa
    10021 tatgaattat gattatctaa atgaacattt tttagagaaa aagggatata aattaggttt
    10081 tactgaaaat actgcaaaat taaacgatgg ctctgaaatt tattatatag aaggtcctaa
    10141 taatggtcca aaacttcttt tgcttcatgg tcagcaagtt agctgctacg attacgctaa
    10201 agtattacca aacctttcaa aagaattcca tgtttttgcc cttgattatt atggtcatgg
    10261 aaaatcatca aaaaatcccg acaagtataa tgctgttgaa ataggaaatg atataatttg
    10321 gtttgttaaa aatgttatta aggaaaaaat ttatatatcg ggacactctt ctggagcctt
    10381 gctagccgca tatgtttctg caaacgcacc tgattgtatt atagctacaa ttttagaaga
    10441 tggtcctttt ttctctactc ttcctaacag ggcggaaaaa actatttcat ggttagaatt
    10501 taaaaatatg tacgactatc taaaccaaaa cgagataggt agttttatgg aatattcatt
    10561 agagcataac tacatgcaag aaatttttaa tgctaaagat ccacaagctt ggaacaaggt
    10621 aataaaaaat cccgccttaa aatatttaga aaaacatccc ggcgaaattc ctattatttg
    10681 gtattatccg ccaaagttgg gaataaatac tatttatgct ttgaatgcaa atatgcagga
    10741 cggaactggt aaatatgatt tgagatttgg actaacattt tatgactttt cgtggtttga
    10801 aggctttgat actgaagaaa ttttgaaaaa tataaaatcg cctactatcg taatgcacgt
    10861 agcaccgagt gatataactg ctccaagtta ttatgatgaa aatggagttt tgcttgctgc
    10921 aatggacaaa aaagatgccc aaaaagttgt tgatttagta cctaatagta aatatgctgg
    10981 aggttttaaa tcagctcacg atattcacgc agacctgcca gacgagttta tagaggtttt
    11041 actcgatttg aaaaatcaaa tagagaaaac aaactaaatt taagatgaaa agagttttta
    11101 taaaagataa aaccaaattt atcaaaagaa ataaagctca taaaatagaa atgttttctg
    11161 aagagctaat aaaagattta cctgagcctt taaaaaaata ccttcgcgtt tgtggatata
    11221 taaacacacc tgttccagta aatgctaata tatattggac tgagtcttgg ttgaaaatgt
    11281 cgccggcaaa agaatggagc gaacttcata caacacaatt taattctgta aagccaatag
    11341 gacggctagc ctatatgaaa attttagaca tgcctgtggc ggcaagagac ttgtatcgag
    11401 atggctatgg agaaatgaaa gttaagctat ttaatttgat aaatctagct ttagataaca
    11461 gtaaagaagt agcacaatcg gcactgataa ctattttttg tgaatttgct tttatcccga
    11521 gttatttgtt gtctgaaaat gtaaaatggg aacagataga cgattattca gttcgcggaa
    11581 ctctcgtaga taacggaatt gaagtttccg gaatgttcta ttttaacgaa gaggggcttt
    11641 ttacacattt tgaaacattc gatcgatatt ttaccacagg caaaaacagc tataagaaag
    11701 ttaagttttc ggttgttgta gaaagtttta aaacacagga taatttaaaa attggagaga
    11761 aagtcaaaat tatttggcat ttgcctgaaa gcgattacga atattataaa gggacggttg
    11821 atagaataga gtttaatgtt taaaataaca tgactgacaa tagtatgaaa atcaaaactt
    11881 taaaaaaata tttgcttaat tgtttcctct tgacaattcc tattatgata tggaatatca
    11941 tctttacaag caaattgcct caatattatc agccagaaat attttggaaa gacattcctg
    12001 cctggttgaa atattctgaa aatatttcaa ggatattgat tttcatactt actttactga
    12061 tgcctctaaa catttcaaca ttgacacaaa aacggggact cattttatat ataggcggaa
    12121 cactaatata ctttgcctct tggcttgtcc tgatttattt tccaaacagt agctggagta
    12181 ataatatcct tggatttatg gcacctgcat acacaccatt attgtggcta acaggaatcg
    12241 gatttgttgg aaattcattt tgtttcaatc tgccctataa acgctggttt tttattttga
    12301 tagtaatcat ttttctgata cttcataatt ttcacacaat gacaatatat ttcagaattc
    12361 attaaaacaa ataaaaaaag ttagaaaaaa taatgaaagt ttatgttata taagtcttta
    12421 aaaataagcg tttaatgtat gtaactaaaa acttaacaga aacttaacac agcttctttc
    12481 aaacttataa gtaagatgag taattttgtc tgtctataaa cttagatgtg tgaaaacgaa
    12541 atcaattttt ttaacaattt tttcgattac gcttttctat atctgttggg gtgtctcaca
    12601 actgataagc atcaagacac agtattcact actgtcaagt ctagtgtttt ctattgtttt
    12661 tactggtcta atcggcactt ttataccaat atatttaaag aataaattta attggagtta
    12721 caataaacca agttcaaaca gactactagc ttatcttttt ttgattttag caattatatt
    12781 ttcaactata ttgtcgggag catttttaaa agtcattgaa ttgaaatatt cttggatttt
    12841 aattttaaaa tatattctac tgtttttccc tatgtcttta ggaattggct tatttgcatt
    12901 tttactaatt ccaaacacta tacaaaactg gaaaaacagc aaaacaaaaa gtgtattgtt
    12961 aattttttca attagcatct ttttctttat agcattttat gttgatacat tatttcaaga
    13021 tatagaatta gcagctacca tggcatttat tggcttattg ttaggcttgg cttatttatt
    13081 tctaagaaat ttttggcttg tttactcaac tcttttttta ataatgttag tgaatacatt
    13141 agccgacaat aaatacgatg aatataattt ttggattgta attataagta ctatactttc
    13201 tttaacaata ttgacatttg attttataaa aaatagaaaa ttaaaaaata ctaacttata
    13261 aataataaaa tatgacaact gaaaaagcaa aatcattgca agctatctta gataaaaagg
    13321 ttgatggcaa aaaaatattt ggaacatctt tcgctttaaa aaaagataat acagtttggc
    13381 aaggagcatc aggtaattta agtagggata ttcagaaatt gttaataaaa agacaacaaa
    13441 ttgattgtta attagtt
//
LOCUS       DAOPNX010000070        13168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_5496_length_13168_cov_9.438039, whole genome shotgun sequence.
ACCESSION   DAOPNX010000070 DAOPNX010000000
VERSION     DAOPNX010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13168
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5496_length_13168_cov_9.438039"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(262..2511)
                     /locus_tag="PKN53_08165"
     CDS             complement(262..2511)
                     /locus_tag="PKN53_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOD88960.1"
                     /translation="MKKIRKLNLFSIFILMSITIYAQSPTIEWQRCLGGISDDKAYSI
                     QQTDDGGFIVAGYTYSKNGDVLENNGRYDYWIVKLNNLRVIKWQKCFGGKGSDCAYSI
                     QQTDDGGFIVAGETSSNDGDVSGNHGKIDYWVVKLNSSGDIQWKKCFGGKGIDKAYSI
                     QQTDDGGFIVAGETSSNDGDVSGNHGKIDYWVVKLNSSGDIEWKKCFGGKGSDKAYSI
                     QQTDDGGFIVAGYTGSNDVDVSGYHGYSDYWIVKLISSGDIEWQRCLGGTGNDGAYSI
                     QQTNDGGFIVAGYTGSNDGDVSGYHGYSDYWIVKLISSGDIEWQRCLGGTGNDGAYSI
                     QQTNDGGFIVAGYTMSDDGGDVSGNHKYDKYNYVLKKNYAYVSYDYWVVKLNIWGNIE
                     WQKCLGGKGIDKAYSIQQTDDGGFILAGYSSSNDGDVSGYHGFSDYWIVKLSSPSGIF
                     FTQNLIENKKIFPNTEKDKNDNIEEEIVVGKSDVDIDIPETKIKKPNTFALIIGNEDY
                     QSYQTGLSSEANVDFAINDAKIFKEYAIKTLGIPEWNIIYLTNGKYVEMSRAITKLNL
                     LAKNTQGNCELIFYYAGHGFPDETTKEPYLIPVDASGEDLQYALKLQDVYTKLIEHSP
                     KRVTVILDACFSGGGRNQGLLAARSVKIVPNKNILSGNLVVFSSSSESQSSLPYRDKQ
                     HGFFTYFLLKKLQETKGNVTYGELSNYLIKEVGVNSVLISGKEQVPEVNSSYEVKDVW
                     QNWKFTDVP"
     gene            complement(2876..4378)
                     /locus_tag="PKN53_08170"
     CDS             complement(2876..4378)
                     /locus_tag="PKN53_08170"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOD88961.1"
                     /translation="MKKIKHLILIILLGCFMYGCATTSKVPFDYTTTKTYPPIDAKIK
                     ITTNIDYNHLKTFWSAITYSKPQTDINNLIITDLRRSIFPIIVENNSDINININIMLK
                     FKDPLAWSFCFPIPVFGSVLGIPTNVLKGKIEIDLQIFSKEGKLIGSYFSKKEIKKNL
                     NFYYYSSIIFETCLATTTTQIMDDIISQIDKDRYKIEEAIKQSKSIVSDNANIETEVG
                     KISDKSTITKPKFYSDVDINIPITTSKNTYRFALIIGNEDYTSKQTGLKTEENVDFAI
                     RDAEIFKEYANKTLGIPEDNIIYLNNATSIEMNRAVAKLNLIIKSLEGKAEIIFYYAG
                     HGYPDPITKEPYLIPVDVTSTDLTYAIKLKDVYAKLTEYPAKKVTVFLDACFSGGGRN
                     QGLLAARGVKIVPKENLLRGNLVVFSSSTGEQSSLPYDEKQHGIFTYYLLKALQEHKG
                     SITYKELSDYLKEKVSIQSAIVNTKEQTPVTNVSIDIQDVWQNWKFSDVP"
     gene            complement(4485..5339)
                     /locus_tag="PKN53_08175"
     CDS             complement(4485..5339)
                     /locus_tag="PKN53_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007756207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HOD88962.1"
                     /translation="MKNLVRFDWAIKRLLRNKANFVILEGFLSELLGEDIKIESILES
                     ESNQDYKNDKFNKVDLLVKNSKGELVIIEIQNNREYDYLLRILFGTSKLLIDNMETGM
                     SYSEIKKIISVSIVYFNLGQGEDYVYHGTTNFVGLHKNDILKLSKDEEILYKTESISK
                     IYPEYYIIKVNQFDDVAKSTLDEWIYFLKKEEIKDEFKAKGLKEAKNKLDVLKLSEHE
                     RKEYERFIEQVRYENSLIVSNYEVGELKGKEEKSIEIAKNLLKAGIDINIIEQSTGLS
                     KEEIEKLK"
     gene            complement(5425..6354)
                     /locus_tag="PKN53_08180"
     CDS             complement(5425..6354)
                     /locus_tag="PKN53_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88963.1"
                     /translation="MKKIIQIVFILFFTISMNLYAQKKDIVIVEGTAQVEYPDYKSLD
                     EVKREAEELAKINALEKAFGIIIIQGNSTYVSNINTGEKTETKSEFTMIGNTFVKGEI
                     LEITEESFKEIIGYKNIDGKEYPIKEIQCTIKAKAIELTEPKIEFEVYTLSGKDKRLK
                     TTSFKSNDTLYFYFKSPVSGYLSIFLDDNKYSQCLLPYKSFTNKNEGGVPIEADKEYI
                     FPLDDFRSCYILHAESMVDLNRIFVIFSKNSFNKPSLKENINYEILSEFEKQKHYITP
                     DGLESEKFQDWLQKNKIFREDFQVEIIDITIKK"
     gene            complement(6375..7028)
                     /locus_tag="PKN53_08185"
     CDS             complement(6375..7028)
                     /locus_tag="PKN53_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88964.1"
                     /translation="MKKVVFLLITVLLSTVIFAQTEKKVDRGLTKEITEKAIKEARKE
                     AKKLAKEGWTVNPGSLPLDKILENAWKKQYTTDEKGLPLYITADGNAVAETKTVAEMQ
                     AIEMGKLQLAGLIETNINSLISGNLANAQISTEDAASITEIVQSSKNLIAMELGYVDP
                     FFKIYRDINDNKVEVQVRLFYDRTQSMEIAKKVIRKELKDKLKINEQQLEKLMGISE"
     gene            complement(7388..8536)
                     /locus_tag="PKN53_08190"
     CDS             complement(7388..8536)
                     /locus_tag="PKN53_08190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOD88965.1"
                     /translation="MSCEREIPESPEGLTNQISSHPNSQQKCEGESVTFSVTVKGIIL
                     SYQWKKNDTNILGANDSTYTISSVSTSDAGNYTCYITSTSGNITSNQATLTVNPPPTA
                     PSSVSASPNPIWSGSSTTLSCSGGSGTTFKWYTGSCGGTYVGQGQNLSVSPTSTTKYY
                     GRWENSCGNSSCKNITVTVSTFTCGTSTITYNGVTYNTVQIGSQCWLKENLKTTHYND
                     GTSIPNVTDNAIWETTTSGAYCCYDNNPSNCNTYGALYNWYAVNTDKLCPNGWHVPSD
                     AEWTTLVNYLGGASVAGGALKEAGTSHWSSPNTSATNSSGFSALPGGNRYGSDGSFYY
                     LGSYGYWWSSTEYDGSNAWSRLLAYDFANVYRYYSYKRVGFSVRCLRD"
     gene            complement(8746..9594)
                     /locus_tag="PKN53_08195"
     CDS             complement(8746..9594)
                     /locus_tag="PKN53_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88966.1"
                     /translation="MKIIRKIFILIIFNLTSLFVFSQSIKNVHSEIKGNQVIVYYSVL
                     GLRYFQTLKTSLYVSMDGGKTYLGPLKAVSGDLEIITNGNKQIVWDVFKDVDKLEGDI
                     VFDVRAEVIEKEREKNWFLSYSGSYYSPYGLQFGQLGKIGWYVSARANSNILKKANYN
                     CNSEGIIDYTGEGYYVFDDKEYLKCYSITAGLTSQWHWNFYWYLGAGYGKNILLWHIN
                     EYSYPNYEKTSTYVKNLDYSYTGVEVETGFIFKIKNKILISVGVANLNFIKDLTEFEF
                     GLGYNF"
     gene            complement(9605..11278)
                     /locus_tag="PKN53_08200"
     CDS             complement(9605..11278)
                     /locus_tag="PKN53_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOD88967.1"
                     /translation="MNLQPSYQRGMPPNLFVEMNFDDDNANGILENNESATLELKITN
                     KGKGPAQGLLVTVKDNYYDPELKIEDKKEIAFIEPGETKNVLIPIKAGFNIKTAEHKL
                     EINVTEHFGYDMDDAYLMFNTLEYQKPELVFSGLEIIDYGEGTAAITKDGQLQPGELV
                     KVKIVVQNIGQNIALNTKYKIVCNNKDIYIENSEGSLGNIGIGEVKEFFIDLSPNKRV
                     IEQEKLPIFLSLKEDIGMGNLNNFQLPIFLNKRPPDPIIKDVKPNIEMMEKQIARFEY
                     KSNKFTANINNVIDIWDVPISKTKRPNSVAVVFGIENYENLAPAPYAKNDAKIIKEYF
                     EKRLGIEQVVIYTNEEVRGFIFDNVFDPYIGELKKAVIDSVSDVFVFYSGHGIPNKDG
                     DRIYLFPYDGKIEKLETQGYDMNKFYNNLEKLNAHSITVFIDACFSGFSRKSEKINDQ
                     NLIGMKGGAKFAPKIYEPWQKNNNFSVFTSSGINETSLGFDPSETGLFTYYLCAGLQG
                     KADANHDNKITFNELKDYVIDNVKTVSLKISGLQTPEFHGNNEMILVEY"
     gene            complement(11380..12303)
                     /locus_tag="PKN53_08205"
     CDS             complement(11380..12303)
                     /locus_tag="PKN53_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88968.1"
                     /translation="MKKIIQIVFILFFTISMNLYAQKKDIVIVEGTAQVEYPDYKSLD
                     EVKREAEELAKINALEKAFGIIIIQGNSTYVSNINTGEKTETKSEFTMIGNTFVKGEI
                     LEITEESFKEIIGYKNIDGKEYPIKEIQCTIKAKAIELTEPKIEFEVYPLSGPDKRLK
                     TTSFKHNDTLYLYFKSPVSGYLTVFLDDNKKAERLLPYKGMTNEYESGVPINADEEYI
                     FFSNDIYCILYAETMYDLTRMFIIFSKNPINKPFLKENVPEEILSNYEKDNGYFTPEE
                     LKSEEFQDWLQKNRYAREDIQLKIIDIIIKK"
     gene            complement(12324..12977)
                     /locus_tag="PKN53_08210"
     CDS             complement(12324..12977)
                     /locus_tag="PKN53_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88969.1"
                     /translation="MKKVVFLLITVLLSTVIFAQTEKKVDRGLTKEITEKAIKEARKE
                     AKKLAKEGWTVNPGSLPLDKILENAWKKQYTTDEKGLPLYITADGNAVAETKTVAEMQ
                     AIEMGKLQLAGLIETNINSLISGNLANAQISTEDAASITEIVQSSKNLIAMELGYVDP
                     FFKIYRDINDNKVEVQVRLFYDRTQSMEIAKKVIRKELKDKLKINEQQLEKLMGISE"
BASE COUNT         4055 a         2324 c         1714 g         5075 t
ORIGIN      
        1 ataaatgaaa atctgtaaaa tattaaattt taattttcct aaatatcaaa ctactaattt
       61 gtttataatt tggatctgat gcgacataaa cagattttac atattttttc acttcttttg
      121 ctatgtcaac ctatttcagg acgagacaac tacacaatct cctctcatag tttacaaact
      181 tgccaactaa agcacgctaa aacatgtaaa cccttctatc tcacacggct tgcttttttt
      241 aaatcaaatt atttgctaat gctatggaac atcagtaaac ttccagtttt gccaaacgtc
      301 ttttacttca taactgctgt ttacttctgg aacttgttct ttaccactaa ttaatactga
      361 atttacacct acttctttta ttaaataatt agatagttct ccataagtta cattaccttt
      421 ggtttcttgt aattttttta acaaaaaata tgtaaaaaaa ccatgttgtt tatctctata
      481 tggcaatgaa gattgagatt cactacttga tgaaaaaact accaaatttc cacttaatat
      541 atttttattt ggaacaattt ttacgcttct agcggctaat aatccttgat ttctccctcc
      601 accactaaag caagcatcca gaataactgt tactcgttta ggagaatgct caattaattt
      661 tgtataaaca tcctgaagtt ttaaagcata ctgcaaatct tcaccacttg catcaacagg
      721 tattaaatat ggttcttttg ttgtttcatc aggaaatcca tgtcctgcat aataaaaaat
      781 taattcacaa ttaccttgtg tgtttttagc taataaattc aattttgtta ttgctctact
      841 catttcaaca tattttccat ttgttaaata aattatattc cattcaggta ttcctaatgt
      901 tttaatagca tattctttga atatttttgc atcatttata gcaaaatcta cattcgcttc
      961 acttgataaa ccagtttgat aactctggta atcttcatta ccgatgataa gggcaaaagt
     1021 atttggtttt tttattttgg tctcggggat atctatgtcc acatcagatt tacctactac
     1081 aatttcttct tcaatattat catttttatc tttttctgta ttaggaaata tttttttatt
     1141 ttctattaaa ttttgtgtaa aaaagatacc tgatggagaa gataatttaa caatccaata
     1201 gtcactaaat ccatgatatc ctgacacatc gccgtcattt gaacttgaat agccagccaa
     1261 aataaatccc ccatcatctg tctgttggat ggaatatgct ttatcaatac cttttccgcc
     1321 gaggcatttt tgccattcaa tatttcccca aatgttaagt ttcactaccc aataatcata
     1381 agatacatag gcataattct ttttaagtac gtaattatat ttgtcatatt tatgatttcc
     1441 tgacacatcg ccgccatcat cggacattgt atagccagcc acaataaacc ccccatcatt
     1501 tgtctgctgg atggagtatg caccatcatt acccgtaccg ccaaggcatc tttgccattc
     1561 aatatctcct gaacttatta gtttcactat ccaatagtca gaatatccat gataccctga
     1621 cacatcgcca tcattggaac ctgtatagcc agccacaata aaccccccat catttgtctg
     1681 ctggatggag tatgcaccat cattacccgt accgccaagg catctttgcc attcaatatc
     1741 tcctgaactt attagtttca ctatccaata gtcagaatat ccatgatacc ctgacacatc
     1801 gacatcattg gaacctgtat atccagccac aataaatccc ccatcatctg tctgttggat
     1861 ggaatatgct ttatcactac cttttccgcc gaaacatttt ttccattcaa tatctcctga
     1921 actattaagc ttaactaccc aatagtcaat tttcccatga ttccctgata catcgccgtc
     1981 attggagctt gtctcaccag ccacaataaa tcccccatca tctgtctgtt ggatggaata
     2041 tgctttatca ataccttttc cgccgaagca ttttttccat tgaatatctc ctgaactatt
     2101 aagtttcact acccaatagt caattttccc atgattccct gatacatcgc cgtcattgga
     2161 gcttgtctca ccagccacaa taaatccccc atcatctgtc tgttggatgg aatatgcaca
     2221 atcactacct tttccaccga agcatttttg ccatttaata acccgcaaat tgttaagttt
     2281 cactatccag tagtcatatc ttccattatt ttctaaaaca tctccatttt tggaatatgt
     2341 atagccagcc acaataaacc ccccatcatc tgtctgttgg atggaatatg ctttatcatc
     2401 acttattccg ccaaggcatc tttgccactc aatagtaggt gactgtgcat aaatggttat
     2461 actcattaaa ataaagatag aaaataaatt taattttctt atctttttca taattttatt
     2521 tgtaaatttt ttcattgctt taaaatttta tacaacaaaa ataaataaaa attaacaaac
     2581 aacaaacaac ctacaactaa caacaaaaac tgtctttttc agggctaaag cccagttagc
     2641 ctacttatta agtccacgac ctaaaggtcg tggttaattg aagttggagt tagataggct
     2701 attatagata tatagccctg aacattatgt caattagcaa taagttagat agcaaagtgc
     2761 tatagaataa ccctgacctt taggtcaggg tacaacaaag gaagaaagat gggctttagc
     2821 cctgaaaaag tatgagcagc attaaggttg atggataaaa aatcatttgc taatactagg
     2881 gaacatcact aaacttccag ttttgccaaa catcttgtat atcaatgctt acattagtta
     2941 caggtgtttg ttcttttgta tttactattg ctgattgtat gcttactttt tcttttaagt
     3001 aatcagatag ttctttatat gttatggaac ctttatgttc ttgtaatgct tttaacaaat
     3061 aatatgtaaa tatgccgtgt tgtttttcat cataaggcag tgacgattgt tcgccagtgc
     3121 ttgaagaaaa gacaactaaa ttacctctta ataaattttc tttgggtact atttttacgc
     3181 ctcttgcagc taataaacct tgatttcttc ctccaccact gaaacaggca tctaaaaaca
     3241 ctgtaacctt ttttgcagga tattctgtta attttgcata aacatcttta agttttattg
     3301 catacgttaa atctgtactt gtaacatcca caggaatgag gtagggttct tttgttattg
     3361 ggtcaggata cccgtgtcct gcatagtaaa aaattatttc tgcctttccc tctaatgact
     3421 ttattattaa attaagtttt gccactgcac ggttcatttc aattgatgtg gcattattta
     3481 agtatataat attatcttca gggataccca gtgttttatt tgcatattct ttaaaaattt
     3541 cagcatctct tatagcgaaa tcaacatttt cttctgtttt taatcctgtt tgctttgagg
     3601 tgtaatcttc attaccgatt attaaagcaa atcgatatgt gtttttagat gtagtaatag
     3661 gtatgtttat atctacatca gaataaaatt tgggttttgt aatagtagat ttatctgaaa
     3721 tttttcctac ttcagtttct atattagcat tatcactaac aatagatttt gattgtttaa
     3781 ttgcttcttc aattttgtat ctgtctttat ctatttgcga tataatatca tccattattt
     3841 gagtagtggt agtagcgaga caagtctcaa aaattataga actataataa tagaaattta
     3901 gatttttttt aatttctttt ttagaaaaat aacttccaat taatttaccc tctttggaaa
     3961 agatttgcaa atcaatttct atcttaccct ttaatacatt tgtagggatt cctaaaaccg
     4021 acccaaaaac agggattggg aaacaaaagc tccaagcaag aggatcttta aattttaaca
     4081 taatatttat attaatatta atatcagaat tattttcaac aattatagga aatatgcttc
     4141 ttcttagatc agttataatt aaattgttta tatcagtttg aggttttgaa tatgttatag
     4201 cactccaaaa agtctttaaa tggttataat ctatattagt tgttatttta atttttgcat
     4261 caataggtgg atatgttttt gttgtagtat agtcaaaagg tacttttgat gttgttgcac
     4321 acccatacat aaaacaaccc aataaaatta ttaatattaa atgttttatc tttttcatct
     4381 atatataatt ttaaaaatgt taaataacaa tatcaaattt aagaaatata ttcattcaca
     4441 ttatgtaaga ttttcaatat tatggatttt cataataagt ttacttattt aagtttttca
     4501 atttcttctt tggaaagtcc tgttgattgc tcaataatat taatgtctat acctgctttt
     4561 aaaagatttt tagcaatttc aatacttttc tcttcttttc cttttagttc acctacttca
     4621 taatttgata caattagact attttcataa cgcacttgtt caatgaacct ttcatattcc
     4681 tttcgctcat gttcactaag ttttaataca tctaacttat tctttgcttc ttttaatcct
     4741 ttagctttaa actcatcttt tatctcttct tttttaagga aatatatcca ctcatctaag
     4801 gtgcttttag caacatcatc aaactgattt actttaatta tatagtattc aggatatatt
     4861 tttgatatac tttctgtctt ataaagtatt tcctcgtctt ttgataattt taatatgtcg
     4921 tttttatgta agccaacaaa attcgttgtc ccatgataaa cataatcctc gccttgccct
     4981 aaattaaaat aaacaatgct tacagatatt attttcttaa tctctgaata actcatccct
     5041 gtctccatgt tatctataag caattttgat gttccaaaaa gtatacgcaa caaataatca
     5101 tattccctgt tattttgaat ttctatgatt actaattcgc ctttagaatt tttaacaagc
     5161 aaatctactt tattgaattt atcattttta tagtcttgat tgctctcact ttctaaaata
     5221 ctttctattt taatatcttc gccaagcaat tcacttaaaa aaccttcgag aataacgaag
     5281 tttgctttat ttcttaaaag tctctttatt gcccaatcaa atcttactaa atttttcatc
     5341 ggtatataat ttttggttca ccaaaaataa ataaaaattt tgaattaaca ttatgtaaga
     5401 tttttaatat tatggatttc attattattt ttttattgta atatctatta tttcaacctg
     5461 aaaatcctct ctaaaaatct tatttttttg caaccaatcc tgaaattttt cagattcaag
     5521 tccatcgggt gtaatataat gtttttgttt ttcaaattct gatagtattt catagttaat
     5581 attttctttt aatgaaggct tattgaatga attttttgaa aaaataacaa atatcctgtt
     5641 taaatccacc atagattctg catgtaatat ataacaagaa cgaaaatcat ctaaagggaa
     5701 aatatactct ttatcagctt caataggcac tccaccttca tttttatttg taaaactttt
     5761 ataaggcaat aaacactgag aatatttatt atcatccaaa aatattgata aataaccact
     5821 tacagggctt ttaaaataga aatataaagt atcattactt ttgaaagaag tcgtttttag
     5881 tcttttatct ttaccagata atgtgtaaac ctcaaattca atttttggtt ctgtcagttc
     5941 tattgctttt gctttgatag tacattgaat ttcttttatt ggatactctt tcccatcaat
     6001 atttttataa ccaattatct ctttaaaaga ctcttctgtt atttctaata tttcgccttt
     6061 aacaaaagta ttaccaatca tagtaaattc agatttagtt tctgtttttt caccagtatt
     6121 gatatttgat acataagttg agttaccctg aataattatt ataccaaaag ctttttcaag
     6181 agcattgatt ttagctaatt cttcagcttc tcgttttacc tcatccaatg atttgtaatc
     6241 agggtattct acctgtgcag tgccttctac tataacaata tctttttttt gagcataaag
     6301 attcattgat atagtaaaaa ataaaataaa tacaatttgt attatttttt tcacttttta
     6361 tttaaaaata atttttattc tgaaatgccc attagttttt ctaattgttg ttcattgatc
     6421 tttagtttat cttttaactc tttacgtata accttcttag ctatttccat agattgcgtt
     6481 ctatcataaa ataacctaac ctgtacttca actttattat cattaatgtc tctgtaaatt
     6541 ttaaaaaatg gatcaacata acccaactcc attgcaataa ggttttttga agattgtaca
     6601 atttctgtaa tagaagctgc atcttctgta cttatttgtg catttgctaa attgccgctg
     6661 ataagtgaat taatattggt ttcaattaat cctgcaagtt gcaattttcc catttctatt
     6721 gcctgcattt cagcaacagt ttttgtttct gccacagcgt taccgtctgc tgttatgtaa
     6781 agtggtaacc ccttttcgtc ggtagtatac tgtttcttcc aagcattttc caatatttta
     6841 tctaatggca acgagcctgg attaacagtc cacccttctt ttgcaagttt tttagcttct
     6901 ttacgtgctt cttttatcgc tttctcagta atttctttag ttaatcctct gtcaaccttt
     6961 ttttcggtct gggcgaatat tacagttgat aataaaactg taattaataa aaacactact
     7021 tttttcatac ttttttattt ttggggtttg atttacaaaa ttatatattt ttttgatata
     7081 aaaaattttt taagaggcaa ttcatgaatt gcccatacgg tgcatttgga tttgtttttc
     7141 agggctaaag ccctaccttt tttatttgct ctttttcatc agcactatct aattcataaa
     7201 tattctcagc cttttttact acttttaact ttatgaactt tttactttca actttctact
     7261 atctttattc gcggcggata agggggggca atgcccccct tataatcccc acaatagtca
     7321 aatttaccct gttaaataag acacagcctt ccgcatagcg gatttaacag ggtaaactca
     7381 aatagtctta atcacgtagg catcgcactg agaacccgac cctcttatag ctgtagtagc
     7441 ggtagacatt agcgaaatcg taggccaaga gacggctcca tgcgttgctg ccatcgtact
     7501 ccgtactact ccaccagtag ccgtagctac caagatagta gaaagaacca tcgctgccgt
     7561 aacggttgcc accaggcaaa gcagagaagc cactagaatt tgtagcacta gtatttggag
     7621 atgaccaatg cgaagttcca gcttctttta atgctccacc agctacactt gctcctccta
     7681 aataattaac taatgttgtc cactcagcat ctgatggtac atgccaccca tttggacata
     7741 atttatctgt gtttactgca taccaattgt ataatgcacc ataggtattg caattagatg
     7801 gattattatc atagcaacaa taggcacctg atgtggtagt ttcccatata gcattatctg
     7861 ttacgtttgg tattgatgtg ccatcattgt agtgtgtagt tttcagattt tctttgagcc
     7921 aacattgaga gccaatttgt acagtgttgt aagtgactcc attatatgta attgtagatg
     7981 taccacatgt aaatgtgctt actgttacag ttatattttt acatgaagaa ttaccgcagg
     8041 aattctccca tctcccataa tattttgttg ttgatgtagg ggataccgat aaattttgac
     8101 cttggccaac atatgtacct ccacatgaac cggtatacca cttaaaggtt gtaccactac
     8161 ctccactgca tgataatgtt gtactgcttc cactccaaat aggattagga ctggcagaaa
     8221 cagatgacgg tgcagtaggc ggtggattta ctgttaaagt agcttgatta cttgtaatat
     8281 ttccacttgt gcttgtaata tagcaggtat aatttcctgc atcgcttgta gaaacagagc
     8341 ttatagtata agtactatca tttgcaccca atatgtttgt gtcatttttt ttccattgat
     8401 aagacaaaat tatccctttt acagtaacac tgaatgtaac gctttcacct tcacattttt
     8461 gttggctatt agggtgagat gatatttggt ttgtcagtcc ttcgggtgac tctggaattt
     8521 ccctttcaca ggacactaca ataataacta tagtaagcat tgtggtaaac gatgatagtc
     8581 ctataatttt tttcatctgt atataatttt tttattcaat aacaaaatta atgaaaatta
     8641 ttcatttaca ttgggtatga tttttaatat tatggatatt tcataattat aatgttttat
     8701 gaccttcctc aaccccttca gaaatgagag gggttagggg tgaggtcaaa aattataacc
     8761 taacccaaat tcaaattcag ttaaatcttt aataaaattc aaattagcta ctcctactga
     8821 aattaagatt ttgtttttta ttttgaaaat aaaacctgtt tcaacttcaa cacctgtata
     8881 agaataatca agatttttaa cataggtgct tgttttttca taattaggat atgaatattc
     8941 attaatatgc catagtagaa tattttttcc atatcctgct ccgagatacc aatagaaatt
     9001 ccagtgccat tgagatgtta aacctgcggt aatagaataa catttaaggt attccttatc
     9061 atcaaatacg taatatcctt cgcctgtata atcaattatt ccctcgctat tacaattgta
     9121 atttgctttt ttaagaatat ttgaatttgc tcttgcagag acataccatc ctatttttcc
     9181 taattgtcca aattgtagtc cataaggtga gtaatagctt ccactataag ataagaacca
     9241 gtttttttct ctttcttttt caattacttc tgccctcaca tcaaatacaa tatcaccttc
     9301 aagtttatct acatctttaa aaacgtccca tacaatttgt ttattaccat ttgttattat
     9361 ttctaaatca ccactaactg ctttaagagg accaagataa gtttttccgc catccatact
     9421 tacataaagc gaagttttca gagtttgaaa gtatcgtaac cctaatactg aataataaac
     9481 aatgacttga ttacctttaa tttcagaatg aacattttta attgattgag aaaaaacaaa
     9541 taaggatgta agattaaaga taattaatat aaatattttt ctgattattt tcattttagt
     9601 aaatttaata ttctactaat atcatttcat tattaccgtg aaattctggt gtttgcagtc
     9661 cgcttatttt tagggaaacg gtttttacat tatctataac ataatctttt aattcattaa
     9721 aagttatttt gttatcatga tttgcatcgg ctttgccctg cagaccggca caaagataat
     9781 aagtaaaaag acctgtctcg gaagggtcaa aaccgagaga agtttcatta atacctgaag
     9841 aagtgaatac gctaaaatta ttgttttttt gccaaggttc ataaatttta ggggcaaatt
     9901 ttgcacctcc tttcattcca ataagatttt gatcatttat tttttcagat ttacgtgaaa
     9961 atccgctaaa acaggcgtca ataaataccg taatactatg tgcatttaat ttttcaaggt
    10021 tattataaaa tttattcata tcataaccct gtgtttcaag tttttctatt ttaccatcat
    10081 aagggaataa atatatcctg tcgccatctt tattcggaat accatggccc gaataaaaaa
    10141 caaatacatc agagacgcta tctattacgg ctttcttaag ttcgccaata taagggtcaa
    10201 aaacattatc aaaaataaaa cctctaactt cttcatttgt ataaataaca acttgttcta
    10261 ttccaagtct tttttcaaaa tattctttaa taattttagc atcattttta gcataaggag
    10321 caggagcgag attttcgtaa ttttctatac caaaaacaac agcaactgaa tttgggcgtt
    10381 ttgtctttga aataggaaca tcccatatat caattacatt atttatattt gctgtaaatt
    10441 tattggattt atattcaaac cgtgctattt gtttttccat catttcaata tttggtttga
    10501 cgtctttaat aattgggtct ggtggtcttt tattaagaaa aattggcagt tgaaaattat
    10561 taagatttcc cataccaata tcttctttta aagaaagaaa aatcggtagt ttttcttgtt
    10621 caattactct tttattagga cttaaatcta taaaaaattc ttttacttct ccaataccta
    10681 tgtttcctaa actaccttca gaattttcaa tataaatatc tttattatta cagactattt
    10741 tatatttagt atttaatgcg atattttgac caatattttg tactacaatt tttactttaa
    10801 caagttctcc tggttgtaat tgtccgtctt ttgtaatagc agctgtgcct tcgccataat
    10861 ctattatctc aagtccagaa aaaaccaatt ctggtttttg atattcaagt gtattaaaca
    10921 ttaaataagc atcatccata tcatagccaa aatgttcggt tacatttatt tccaacttgt
    10981 gctctgctgt tttaatgtta aaacctgctt ttataggtat aagcacattt ttagtttcac
    11041 ctggctcaat aaaagcaatt tcttttttgt cttcaatctt taattcagga tcataataat
    11101 tatcttttac agtaaccagt aaaccctgag caggtccttt acctttattt gtaattttta
    11161 actcaagtgt tgctgattca ttattttcta aaataccatt tgcattatca tcatcaaaat
    11221 tcatttcaac aaataaattg ggtggcatac ctctttgata agaaggttgt aaattcattt
    11281 tttggggatt gcttttacca cttatgattt cttgggcatg tatattaata aatgccaaac
    11341 acagaaataa aaaaaatagg gcttttaatt tcataagatt cattttttta ttattatatc
    11401 aattatttta agttgaatat cttctcttgc atatcggttt ttttgcaacc aatcttgaaa
    11461 ttcttcggat ttaagttctt ctggtgtaaa atatccatta tccttttcat agtttgatag
    11521 tatttcttct ggaacattct ctttcaaaaa aggcttgttt attggatttt ttgaaaaaat
    11581 aatgaacatt cttgtcaaat catacatggt ttcagcatac aaaatacaat aaatatcatt
    11641 agagaaaaag atatattctt catcggcatt tataggcact ccgctttcgt attcatttgt
    11701 cataccttta tatggcaata aacgttcagc ttttttattg tcatccaaaa acacagttaa
    11761 ataaccactt acagggcttt tgaaataaag atataaggtg tcattatgct tgaaagaagt
    11821 tgtttttagt cttttatctg gaccggataa tgggtaaacc tcaaattcaa ttttgggttc
    11881 tgtcagttct attgcctttg ctttgatagt acattgaatt tcttttattg gatactcttt
    11941 cccatcaata tttttataac caattatctc tttaaaagac tcttctgtta tttctaatat
    12001 ttcgccttta acaaaagtat taccaatcat agtaaattca gatttagttt ctgttttttc
    12061 accagtattg atatttgata cataagttga gttaccctga ataattatta taccaaaagc
    12121 tttttcaaga gcattgattt tagctaattc ttcagcttct cgttttacct catccaatga
    12181 tttgtaatca gggtattcta cctgtgcagt gccttctact ataacaatat cttttttttg
    12241 agcataaaga ttcattgata tagtaaaaaa taaaataaat acaatttgta ttattttttt
    12301 cactttttat ttaaaaataa tttttattct gaaatgccca ttagtttttc taattgttgt
    12361 tcattgatct ttagtttatc ttttaactct ttacgtataa ccttcttagc tatttccata
    12421 gattgcgttc tatcataaaa taacctaacc tgtacttcaa ctttattatc attaatgtct
    12481 ctgtaaattt taaaaaatgg atcaacataa cccaactcca ttgcaataag gttttttgaa
    12541 gattgtacaa tttctgtaat agaagctgca tcttctgtac ttatttgtgc atttgctaaa
    12601 ttgccgctga taagtgaatt aatattggtt tcaattaatc ctgcaagttg caattttccc
    12661 atttctattg cctgcatttc agcaacagtt tttgtttctg ccacagcgtt accgtctgct
    12721 gttatgtaaa gtggtaaccc cttttcgtcg gtagtatact gtttcttcca agcattttcc
    12781 aatattttat ctaatggcaa cgagcctgga ttaacagtcc acccttcttt tgcaagtttt
    12841 ttagcttctt tacgtgcttc ttttatcgct ttctcagtaa tttctttagt taatcctctg
    12901 tcaacctttt tttcggtctg ggcgaatatt acagttgata ataaaactgt aattaataaa
    12961 aacactactt ttttcatact tttttatttt tggggtttga tttacaaaat tatatatttt
    13021 tttgatataa aaaatttttt aagaggtaat tcattaattg cacatacggt gcatttggat
    13081 ttgtttttca gggctaaagc ccagttagct tatttattaa gtccacgacc taaaggtcgt
    13141 ggttaattaa taaggaaact atcttaca
//
LOCUS       DAOPNX010000071        13058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_5555_length_13058_cov_7.490579, whole genome shotgun sequence.
ACCESSION   DAOPNX010000071 DAOPNX010000000
VERSION     DAOPNX010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13058
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5555_length_13058_cov_7.490579"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..540
                     /locus_tag="PKN53_08215"
     CDS             160..540
                     /locus_tag="PKN53_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88970.1"
                     /translation="MKRIGLLVAIVAFALCLVSCGGSGPTADAKKMLKLTQDLTATIN
                     KAAEDKTIADDEAKKINDGLKEFFDFVKKVDEKYKDNEEAQKEFEEYLDTEENEKLGT
                     AFEEAMGKLFECEGFEKISFEGFM"
     gene            complement(749..1108)
                     /locus_tag="PKN53_08220"
     CDS             complement(749..1108)
                     /locus_tag="PKN53_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOD88971.1"
                     /translation="MNIFIGSLPYSVKDSDLQSFFEEYGEVSVARIITDKFTGKSKGY
                     GFVEMKDDEAAKKAIEELNGAELEGRTIVVSQAHERTENHNERKGHYNPKRREHTQRR
                     GDYKQNYENKKHDDFEE"
     gene            complement(1259..1969)
                     /gene="trmB"
                     /locus_tag="PKN53_08225"
     CDS             complement(1259..1969)
                     /gene="trmB"
                     /locus_tag="PKN53_08225"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOD88972.1"
                     /translation="MSAKRKLEKFAQNKTFEHFFEPQFNFQNPSDYEMKANWAKDFFK
                     NENSLIVELGCGKGEYTVGQAKQYPQNNYIGVDIKGARMWRGASDVLNLGLKNVAFLR
                     TRVEFTPFCFAKNEVSEIWITFPDPQKGPRKRIRKRLTSSEFLSRYQKFLIDDGFVNL
                     KTDDDTLYEYTKSVVSLNNLEVIVDTDNLYESKYYTGALTIKTHYEKMWNQENRIIKY
                     LKFKLPQNLKLIEPPYEE"
     gene            complement(1977..2459)
                     /locus_tag="PKN53_08230"
     CDS             complement(1977..2459)
                     /locus_tag="PKN53_08230"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HOD88973.1"
                     /translation="MKKRIKKVFILLIVFSNVFFISCDRNKVYEGFEEIPGDVWDNKQ
                     KINFVFEIDDTLALHNVYIHIRHASNYQYKNIWLFVKSSAPNGLVQIDTLECNLADDL
                     GKWYGKGGDILNLQMPWKMNVRFAHKGKYSVEYEQAMRMEQLPGIAQMGLRVEKVDAK
                     "
     gene            complement(2443..3606)
                     /gene="ricT"
                     /locus_tag="PKN53_08235"
     CDS             complement(2443..3606)
                     /gene="ricT"
                     /locus_tag="PKN53_08235"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOD88974.1"
                     /translation="MADREYERGCLVDKVDETTSNVSRDFCCYKLNVFDWLGDIDDPL
                     AQNKYIEVRFKNTRKEIFLNASDLQLKIGDIVAVEASPGHDIGIVSAIGNLANQHVNR
                     QKFAKDQSFKKIYRKARAHDIQKWKDAISLEIPTMLRTRELADSLGLEMKIGDVEFQG
                     DKTKAIFYYIAEDRVDFRELIKKMAEEFKIRIEMKQIGARQEAGKIGGIGPCGRELCC
                     TTWIYDFVSVSTNSARLQELSLNPQKLAGQCSKLKCCLNYELEVYLDARKNFPDDDIN
                     LKLAEGEAIHIKNDVFKNLMYYEIKINDIPVIRALDVDKVKEIIGLNKKGIFPEKVFD
                     ENSKVSPKISLSFKEELNSDSLTRFDTPQKPKQKNRRNKNRRTKYRKPNEKKN"
     gene            complement(3611..4732)
                     /locus_tag="PKN53_08240"
     CDS             complement(3611..4732)
                     /locus_tag="PKN53_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOD88975.1"
                     /translation="MKFEEILGQSEIKEKLIQSYVNNRVSHSYLFFGTAGSGKLGLAI
                     AFAQYLSCTNKQENDSCGQCPSCKKYEKLIHPDLHFVFPVITEAGKKKISDAFISEWR
                     EMILSDVYVSYNEWMQNLGSQNKQGLISVDESAEIIRKLSLKSFESEYKIMIIWLPEK
                     MHVSCANKLLKILEEPPHNTIFILVSDERESVLPTILSRTQAVKILGIDDDIMEKHII
                     DKYNLSAQEAKDIVRISNGSYVEAKQQILANEENKFNLKQFIHFMRLTYSRNIAEALT
                     WAEDMAKIGREPQKSFLKYSSILIRENFVYNFNDNNIYYLTKEEEEFARNFARFLNSE
                     NVNSIAKIFDDAHYGIERNGAAKIIFTDIAFLVMRVIRK"
     gene            4806..5753
                     /locus_tag="PKN53_08245"
     CDS             4806..5753
                     /locus_tag="PKN53_08245"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOD88976.1"
                     /translation="MQIIIEQILVLALIAIIGLIAFKLKAITKEHNAGLVKVILKITL
                     PILIFTTFANTNLDKEIIQNFPIVFVSAFISVILLYFLSNISAKILKLDKENKALHNT
                     HTMFGNTIFLGFPLLDALFPGGEGLIYATIFQLAHDSLMWTWGIVIMSKASNKNLEKN
                     NKKHLLNPISLAFAVGLIFMIFKIKLPDLILKPLLGVGHTTIYISMIYVGAVLAQAKL
                     KPLIQNLRSYILSFNKLIFAPLVLALILFLVKSLGLNISDTAFICAVLQSAMPCMIII
                     SVLAEDLGLNAKQAVENILVSSVLSIFTLPFIYWVISIF"
     gene            complement(5901..6767)
                     /gene="prmC"
                     /locus_tag="PKN53_08250"
     CDS             complement(5901..6767)
                     /gene="prmC"
                     /locus_tag="PKN53_08250"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOD88977.1"
                     /translation="MKTIEQYSLEIQDLLPKFYDFEERKSIAEFYIKEILDFNNTEYL
                     LKKSEVLSSEEFLKLENKKDRLISGEPVQYVLNKAYFYDLEFFVDKRVLIPRQETEIL
                     VDKIIKFYSTKENLKILDIGTGSGNIAISLKKNLKNAELSVVDISASALDVCSKNADY
                     HNVDLCFYCCDILSEEDKFFEQVFFDIIVSNPPYVTNSEKKLMSKNVVDYEPEKALYV
                     SDDNPLVYYEAITKFAKQHLKSGGVLFFEINEMFGKEVAEICENYGFDEVEIIQDFNA
                     KDRFVIATVKSL"
     gene            complement(6879..7442)
                     /locus_tag="PKN53_08255"
     CDS             complement(6879..7442)
                     /locus_tag="PKN53_08255"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOD88978.1"
                     /translation="MKEEIVAEAIKVLKNSGTILYPTDTIWGIGCDALDEVAVRNIYT
                     IKYRDITKSMLILVDSIEMLANYVEEVPAMALKLIDASTRPLTIIYPKPKKIAKNLIA
                     ADNTIGIRVTRDEFCKSLIKEFGRPIVSTSANLSGQNPPLGYFDVSNEIKMAVDYIVP
                     LRLDELSKARSSDVVRVLKNGEIEIIR"
     gene            complement(7439..8548)
                     /locus_tag="PKN53_08260"
     CDS             complement(7439..8548)
                     /locus_tag="PKN53_08260"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOD88979.1"
                     /translation="MKIAFDAKRAFSNKSGLGNYSRNIIRALHEYYPQNSYYLFSPNP
                     ETDLLEEKYRTNTISPKTKNSILKSYWRSFKMGNDIAEINPDIYHGLSNELPKNIAKT
                     KAKKIVTIHDLIFLKLPHLYKCVDRKIYYKKFKFACDNADKIIATSIQTKNDIIEEFK
                     INPEKLEVVYQSCNLDFSQKYSEKDFDRIRKKYNLPEKYILTVGTIEERKNALQVLKA
                     IFYFNFDLDYVLVGRKTKYLNEIIAWAEDHKIKHKLHVLNNVSNEDLPIIYKLSKVFA
                     YPSIYEGFGIPIIEAFSSEVPVITAEFGACSEIAGQAALKVDVFNPKSIGLAIKNILE
                     DEKLKLELIEKAKLRVKDFSIENMIKNLYRVYQNL"
     gene            complement(8567..9388)
                     /locus_tag="PKN53_08265"
     CDS             complement(8567..9388)
                     /locus_tag="PKN53_08265"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOD88980.1"
                     /translation="MFMNLETIINSAWDDRALLAKPEVESAINEVISLLDSGKLRVAQ
                     PVSAHEWKVNDWVKKAVIMYFQIRKMEKTELGIFEYHDKIPLKKNYDKLGVRVVPHAI
                     ARYGSYLAKGVIMMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHISGGVGIGGVL
                     EPVQAAPVIIGDNCFIGSRCIVVEGVRLENEVVLGANVVITGSTKILDVSGKDVVEYK
                     AFVPERSIVIPGNITKKFPAGEFSVPVALIIGKRKESTDKKTSLNDALREHAVSV"
     gene            9509..10780
                     /gene="purD"
                     /locus_tag="PKN53_08270"
     CDS             9509..10780
                     /gene="purD"
                     /locus_tag="PKN53_08270"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOD88981.1"
                     /translation="MNILVLGSGGREHALTYKIVQSKLAKKVFVAPGNAGTNKIAKNI
                     NLNPLNFEEVSSFIENNKVELLVVGPEAPLVAGITDFLKSLDKHKNLLIIGPSKEAAM
                     LEGSKDFAKAFMQKYNIPTAAHFSANKENLAQAINFLKTLKPPYVLKADGLAAGKGVL
                     IINELSEAISELENMLNGKFGQASSKVVIEEFLKGIECSVFVLTDGKSYVILPEAKDY
                     KRIGDGDTGLNTGGMGAVSPVPFYTKEFAQKVKNQIITPTVNGIKNENFDYCGFIFFG
                     LMNVDGNPYVIEYNVRMGDPETEAVLPRINSDFLTLLVNAGNKNLSKTKIEISEQVAV
                     SIVVASGGYPEDYEKDKLITGIELVSESIVFHAGTKLNGNNIVSSGGRVLAVTSLAKT
                     IQDAVAKSYKSIDKIHFDKMNFRKDIGQDLK"
     gene            10786..11769
                     /locus_tag="PKN53_08275"
     CDS             10786..11769
                     /locus_tag="PKN53_08275"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HOD88982.1"
                     /translation="MLKSNSPLNYLLIFGIMLVFWAFKFVFMPSGVENYEVYGLFQIN
                     FGHRLIYQYLNTFIGFVIYFLLALLLVKANSDLQIVENTYQSPGMVFAFLSGAFINIQ
                     KYNNVLISCLLVSISIAIILYSIKKHIALDNAFNAGLIFAIATIFYPKTIVFLPILIA
                     SMFIVKPTVAREVISLFLGILTPLILFFSYFWLFGDIDSIILKTKLSFTQDFIYIRFS
                     YYNKFIFVPLIFICIYAIIAKFTLSVSQKLNTRKFQTIILIYSIYSLIVFLIPFMPDE
                     TVVFLYPSISLLLTLVLVNSKAKILKIISLIAFAGLIVSHLFQIYFYLSIF"
     gene            11908..12969
                     /locus_tag="PKN53_08280"
     CDS             11908..12969
                     /locus_tag="PKN53_08280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOD88983.1"
                     /translation="MKKLSEEEHKRIQLWAEATEQLINVSPDQDVSFLFEVLKNNETI
                     PVILVDMDDNIISFRNIDSVKMQNPAYVKKKITEFKNNSNENFIIKTEDGEALNQIYY
                     SDSYLLTLLYYYPYFQLGLVFLFILASYFAFSVSKRAEQDQVWLGMSKETAHQLGTPI
                     SSLMAWIEILRDQVKDPEIINELEKDTKRLEKIASRFSKIGSKPNLSEENLRQTLLST
                     INYMTLRIPSSIKISLVNPLKDTDTKVKINEDLFEWVIENLCKNAVDAIGEKGEISIS
                     YEETHKHIYIDIKDTGKGIAKSEQKTIFNPGYTTKARGWGLGLSLVKRIVEQYHQGKI
                     FVKSSELGIGTTFRIVLKK"
BASE COUNT         4321 a         2155 c         1731 g         4851 t
ORIGIN      
        1 agtccgaata ttgaaagcaa cgaagcagtt tgacacttat gagacaccgc ctttttttat
       61 acatatatag aataaagttt ataacaagaa tttggttttt attattaatt gtatttcctt
      121 tacacctaaa ttattattaa caaaattaaa tttaaattta tgaaaagaat tggattatta
      181 gtagcaattg tggcatttgc tttatgcctt gtttcttgtg gtggaagtgg tcctactgca
      241 gacgctaaaa aaatgttaaa attaactcaa gaccttacag cgacaataaa caaagctgct
      301 gaagacaaaa caatcgctga cgatgaagct aaaaaaatta atgatgggct taaagaattt
      361 tttgatttcg taaaaaaagt agacgaaaaa tataaagata acgaagaagc tcaaaaagaa
      421 tttgaagaat atcttgatac tgaagaaaac gaaaaattag gtaccgcttt tgaagaagca
      481 atgggtaaat tattcgagtg cgagggattt gaaaaaatta gttttgaagg ctttatgtaa
      541 aaagcagaat tttttaaaag ataaaaaagt tatcccaatt caggataact ttttttgttc
      601 ctaatattcc gaactaacag gaaatatttt atcatcttat aaatatccaa ttaccgactt
      661 caacattcag ttcaatcgct ttgtatgtat tttagtgtta gagttttaaa aaaagaggaa
      721 atagaataag tagatttaaa attttatctt attcttcaaa atcatcatgt tttttgtttt
      781 cataattttg tttataatct ccacgtcttt gtgtatgttc acgacgttta gggttataat
      841 gtccttttct ctcattatga ttttctgttc tttcgtgtgc ttgactaaca acaattgtgc
      901 gtccttcaag ttctgcaccg tttaattctt caatagcttt tttagcagcc tcatcgtctt
      961 tcatttctac aaaaccatag cctttgcttt ttcctgtaaa tttgtcagta ataattcttg
     1021 ctacgctaac ttctccgtac tcttcgaaga atgattgtaa atcgctgtcc ttcactgagt
     1081 aaggtaagct tccaataaaa atattcattc taaaatttta attaaattaa gatgcaaaga
     1141 taatcaaata atttagaaca acactaaaat aaatggaaaa aataaaacaa atttgcacat
     1201 ctcacaatat gctaataatc aatgacttac actgaaagac ttttccccat tccctccctc
     1261 actcctcata tggcggctca attaatttta aattttgagg taacttgaat tttaaatatt
     1321 ttataattct attttcttga ttccacattt tttcataatg tgtttttatt gttaaagctc
     1381 ctgtatagta tttagattca tacaaattat ctgtatcaac tattacttcc aaattgttta
     1441 aggaaacaac agattttgta tattcataca aagtgtcgtc gtcagttttt aaattaacaa
     1501 agccatcatc aattaaaaat ttttggtatc tagataaaaa ctccgaagaa gtcaatcttt
     1561 ttctaattct tttgcgtggt cctttttgtg ggtctggaaa agtaatccaa atttcgctaa
     1621 cttcgttttt agcaaagcaa aatggagtaa attctactct tgttcgtaaa aacgcaacat
     1681 tttttaaacc caagttcaag acatcgctag ctcccctcca cattcttgct ccttttatat
     1741 ccacacctat ataattattt tgtggatatt gctttgcttg tccaacagta tattctcctt
     1801 ttccacaacc taattcaaca attaaagaat tttcattttt aaaaaaatcc ttagcccaat
     1861 tagctttcat ttcataatca ctaggatttt gaaaattgaa ttgaggttca aaaaaatgct
     1921 caaacgtttt gttttgtgca aacttttcta actttctttt tgctgacatt tataatttat
     1981 tttgcatcaa ctttttctac tcttaaaccc atttgcgcta tacccggcaa ttgctccatt
     2041 cgcatagcct gttcatattc aaccgaatat tttcctttat gagcaaacct aacattcatt
     2101 ttccaaggca tttgcaaatt taaaatatcg ccaccttttc cataccattt tcctaaatcg
     2161 tcggctaaat tacactcaag agtatctatt tgaactaaac catttggtgc cgaagacttt
     2221 acaaaaagcc atatgttttt atactgataa ttagaagcat gtctaatatg tatataaaca
     2281 ttgtgaagag caagagtgtc atctatctcg aacacaaaat ttattttttg tttattgtcc
     2341 cacacatcac ccggtatttc ttcaaagcct tcatatacct tatttctatc acaagaaata
     2401 aagaaaacat ttgaaaacac aattagtaaa ataaaaactt ttttaattct ttttttcatt
     2461 tggcttgcgg tatttagtac gtctgttttt gtttcttcta tttttttgtt ttggtttttg
     2521 aggagtatca aacctagtta aactatctga atttaattct tctttaaaac tcaaactaat
     2581 tttagggcta actttactat tttcatcaaa aaccttttca gggaaaattc cttttttatt
     2641 taatcctatt atttctttta ctttatcaac atctaaagct ctaataacag ggatgtcgtt
     2701 tatcttaatt tcataataca ttaaattttt aaagacatcg tttttaatat gaatagcttc
     2761 tccctctgct aatttaagat ttatatcgtc gtctggaaaa ttttttcttg catctaaata
     2821 aacttcaagt tcataattta aacaacattt aagtttactg cattgtcctg caagtttttg
     2881 aggatttaaa gacagctctt gcaaccgtgc cgaatttgtt gaaacggaaa caaaatcata
     2941 tatccaagta gtgcagcaaa gttctcttcc gcaaggacca attccgccaa tttttcctgc
     3001 ttcttgtctt gctcctattt gcttcatttc aattctaatt ttaaactctt cagccatttt
     3061 tttgataagc tctctaaagt caactctatc ttcggcaatg taataaaata tggcttttgt
     3121 cttatctcct tgaaactcca catcgccaat tttcatttcc aaaccaaggc tatcggcaag
     3181 ttcacgcgtt ctgagcattg ttggaatttc aagagaaatc gcatctttcc atttttgaat
     3241 atcatgagcg cgagcttttc tatatatttt tttaaagctc tgatcttttg caaatttttg
     3301 tctattcaca tgttgatttg caagatttcc aatagccgac actattccaa tatcatgacc
     3361 cggagaagct tctactgcaa caatatctcc aattttcaac tgtaaatcgc tagcatttaa
     3421 aaatatttct tttctagtat ttttaaatct tacttcaata tatttatttt gtgctagagg
     3481 atcgtcaata tctcctaacc aatcaaaaac attaagtttg taacaacaaa aatctctgct
     3541 tacgttagac gttgtttcat caactttatc aactaagcaa cctctttcat attctctatc
     3601 tgccatttta ctattttcta attactctca ttaccaaaaa agctatatct gtaaagataa
     3661 tttttgctgc tccatttctt tctatgccat aatgagcatc atcaaaaatt tttgcaatac
     3721 tgttcacatt ttcactgttt aaaaacctag cgaaatttct tgcaaactct tcttcttctt
     3781 ttgttaggta ataaatatta ttatcattaa aattataaac aaaattttct ctaatcaaaa
     3841 tactactgta tttcaaaaaa cttttttgcg gttctctacc tattttagcc atgtcttctg
     3901 cccatgtcaa agcttctgca atatttcgcg aatatgttaa tcgcataaaa tgaataaatt
     3961 gctttaagtt aaacttattt tcttcatttg ccaatatttg ttgcttagct tcaacataac
     4021 tgccattgct tattctcaca atatcttttg cttcttgagc cgagagatta tatttgtcga
     4081 taatatgctt ttccattata tcatcgtcaa ttcccaaaat ttttaccgct tgagttctcg
     4141 acaaaattgt tggaagtaca gattcccttt cgtcgcttac caatataaat attgtattgt
     4201 gaggaggctc ttctaatatt tttaataatt tattggcaca agaaacgtgc attttttctg
     4261 gcaaccaaat tatcattatt ttatattccg actcaaaaga ttttaagcta agttttctaa
     4321 taatttcggc agattcgtca accgaaatta aaccttgctt gttttgagag cctaaatttt
     4381 gcatccattc gttatatgaa acataaacat cgcttaaaat catttctctc cattcggaaa
     4441 taaaagcatc agaaattttc tttttgcccg cttccgtaat tactggaaat acaaaatgta
     4501 aatcggggtg aattaatttt tcatattttt tacacgaagg acattgccca caactatcgt
     4561 tttcttgttt gtttgtgcat gataaatatt gagcaaaagc aattgccaag ccaagttttc
     4621 ccgaacctgc agttccaaaa aacaaataag aatggcttac tctattattc acataactct
     4681 gaataagctt ctcttttatt tcactttgac ctaatatttc ttcaaatttc ataagcaaaa
     4741 atcacaagat acaaaattaa acaaaaattt caattcatat tcttattaat ttataatttt
     4801 gccaaatgca aataattatt gagcaaatat tagttttagc actaattgcc atcattggtt
     4861 taattgcctt taagctaaaa gccataacca aagaacataa tgctggctta gtaaaagtga
     4921 ttttaaaaat tactcttcca attttaattt tcacaacttt tgctaacaca aatcttgata
     4981 aagagattat tcaaaacttt cctattgttt ttgtttctgc ttttataagc gtgattttgc
     5041 tatattttct cagtaatatc agtgctaaaa ttttaaaatt agataaagaa aacaaagccc
     5101 tgcacaacac tcacacaatg tttgggaata caatttttct tgggtttccg cttttagatg
     5161 cactttttcc tggtggcgaa ggtttaattt atgcaacaat ttttcaatta gctcacgatt
     5221 cgcttatgtg gacttgggga attgtaataa tgagcaaagc ttcaaataaa aatcttgaaa
     5281 aaaacaataa aaaacacttg ctaaatccga taagtcttgc ttttgctgtg ggtttaattt
     5341 ttatgatttt taaaataaaa cttccagatt taattttaaa acctttactt ggcgttggac
     5401 atacaactat ttatatatca atgatttacg ttggagcggt tttagctcaa gcaaaattaa
     5461 aaccattaat tcaaaatttg cgttcttata ttttaagttt taataaatta attttcgctc
     5521 ctttagtttt agcccttatt ttatttttag ttaaaagtct tgggttaaat atttcagaca
     5581 ctgcttttat ttgtgcagta cttcaatcag caatgccttg catgataatc atttccgtat
     5641 tagccgaaga cttaggatta aacgcaaaac aagcagttga aaatattttg gttagctctg
     5701 tgctttcaat ttttaccttg ccgtttattt attgggtaat ttcgattttt tagtgttgag
     5761 tttttagtgt tgagttttta atgttgagtt tttagtgttg agtttttagt gttgagtttt
     5821 tagtgtttgg taaaacttta aaaacttttg ctttcagcga gctcgtaaac tcggttcgaa
     5881 cttgatgaac tttacaaact ttataaactt ttaactgtcg ctattacaaa cctgtcttta
     5941 gcattaaaat cttgaataat ttcaacctca tcaaagccat aattctcgca aatttcagca
     6001 acttcttttc cgaacatttc atttatttca aaaaataaaa ctccgcctga ttttaaatgt
     6061 tgttttgcaa attttgtaat tgcttcataa tataccaaag gattatcgtc tgaaacataa
     6121 agtgcttttt ctggttcata atcaacaaca ttttttgaca ttagtttttt ttctgaattt
     6181 gttacataag gtggatttga aactataata tcaaaaaata cttgctcgaa aaatttatct
     6241 tcttccgata aaatatcaca acaataaaaa cacaaatcca cattgtgata atcagcattt
     6301 ttactgcaaa catctaaagc tgatgcagaa atatcaacta cgctaagttc tgcatttttt
     6361 aaattctttt ttaaactaat agcaatattt cctgaacctg ttccaatatc aagaattttt
     6421 aaattttctt tggtagaata aaacttaata attttatcaa ctaaaatttc tgtttcttga
     6481 cgtggaatta atactcgctt gtcaacaaaa aactctaaat cgtaaaaata agccttgttt
     6541 aaaacatatt gaactggctc tccactaatt aatctgtctt ttttattttc aagctttaaa
     6601 aattcttcac tgcttaaaac ttcgctcttt tttaataaat attcggtatt attgaaatct
     6661 aaaatctctt ttatataaaa ttctgctatt gattttcttt cttcaaaatc ataaaatttt
     6721 ggaagcaaat cctgtatttc caagctatat tgctctatag ttttcaatct aaaaaatttt
     6781 tggcactaaa tttggttatt tataaatagt aacttttttg aaaatgtctg aatttcctcc
     6841 gatgtcaatt cttagcccta agaaagtcgg aggttttttt atcttattat ttcaatttca
     6901 ccatttttta aaactctaac aacatctgag cttcgtgctt tacttaattc atctaatctc
     6961 aaaggcacaa tataatccac tgccattttt atttcatttg acacatcaaa ataacccaaa
     7021 ggcggatttt gtccagaaag atttgccgaa gtcgagacta ttggtcgtcc aaattcttta
     7081 attagagatt tgcaaaactc gtctcttgta accctaatac caatggtgtt gtcggcagca
     7141 attaaatttt tagcaatttt tttcggttta ggataaatta ttgttaaagg tcgagttgaa
     7201 gcatctatta gttttaaagc cattgctgga acttcttcaa catagtttgc caacatttct
     7261 atagagtcaa ccaaaatcaa catgcttttt gttatatctc tatatttaat tgtataaata
     7321 tttctaacag ccacttcatc taaagcatcg caaccaatac cccaaattgt gtctgtagga
     7381 tataatattg ttccactatt tttaagcact tttattgctt ctgccactat ttcttctttc
     7441 ataaattttg ataaactcta tataaatttt taatcatatt ttcaatgcta aaatccttta
     7501 ctcgcaattt tgctttttcg ataagttcta attttagctt ttcgtcttct aagatatttt
     7561 ttattgctaa gccaatgctt ttgggattaa aaacatcaac ttttaaagct gcttgtcctg
     7621 caatttcgga acaagctcca aactctgctg taattactgg aacttctgaa ctaaacgctt
     7681 ctataattgg aattccaaaa ccttcgtaaa ttgaaggata tgcaaatact ttgctaagtt
     7741 tataaattat tggtaaatct tcattgctta cattattcaa aacatgaagt ttatgcttaa
     7801 ttttatgatc ttctgcccaa gcaataattt cattcaaata ttttgttttc cgtcccacta
     7861 aaacataatc taaatcaaag ttaaaataaa aaatagcttt taacacttgc aaggcatttt
     7921 ttctttcttc aattgtgcct acagttaaaa tatatttttc gggcaaatta tatttttttc
     7981 gtattctatc aaaatctttt tcagaatatt tttgcgaaaa atctaaatta caagattgat
     8041 aaactacttc taatttttcg gggtttattt taaattcttc aattatatca ttttttgttt
     8101 gaatacttgt agcaatgatt ttatcggcat tatcacaagc aaatttgaat tttttataat
     8161 aaatttttct atctacacat ttataaagat gtggtaattt caaaaaaatt aaatcgtgaa
     8221 ttgtaacaat ttttttagct tttgttttag caatattttt aggaagttcg ttgcttaatc
     8281 catgataaat atctggattt atttctgcaa tatcgtttcc cattttaaaa cttcgccaat
     8341 aagattttaa aattgaattt ttagtttttg gactaatagt atttgtccta tatttttctt
     8401 ctaacaaatc ggtttcggga tttggagaaa ataaataata ggaattttgc ggataatact
     8461 catgcaaggc acgaataata ttgcgactat aatttcctaa accactttta tttgaaaaag
     8521 ctcgttttgc gtcaaaagct attttcataa ttcacttatt attttcctaa accgacacag
     8581 catgttctct taatgcatcg tttagcgaag tttttttatc agttgattct tttcgttttc
     8641 ctattatcaa agctacaggc acactaaact ctcctgctgg gaacttttta gttatatttc
     8701 ctggaataac tatagacctt tctggaacaa aagctttgta ttctaccaca tcttttcctg
     8761 aaacatctaa aatttttgtt gaacctgtta ttaccacatt tgctcctaaa actacttcat
     8821 tttctaatct tacaccttcc accacaatgc aacgcgagcc tataaagcaa ttgtcgccaa
     8881 taataactgg tgcagcttgt acgggttcta aaactccgcc aattcctact ccgcccgata
     8941 tatgcacatt ttttccaatt tgagcacaag aaccaacagt tgcccaagtg tcaaccattg
     9001 ttccctcatc tacataagca ccaatattta cataagaagg catcattatt actccttttg
     9061 ccaaataaga accatagcga gcaatagcat gtggtacaac acgaactcca agtttgtcgt
     9121 aatttttttt caatggaatt ttatcatgat attcaaaaat gccgagttct gttttttcca
     9181 tttttctaat ttggaaatac ataattactg ccttttttac ccaatcgtta actttccatt
     9241 cgtgagccga aacaggctga gcaactctca actttccaga atccaacaag ctaattacct
     9301 catttatagc cgattccact tcaggctttg ctaataaagc tctatcgtcc caagccgaat
     9361 ttataatagt ttctaagttc ataaacacat ttttttaatt ttccaaagtt aataaattta
     9421 gacaaaagat tagtcaaaca aaatattatt tctattctaa acaaaaaata agtattttta
     9481 ccaactaaaa taaattgcta agattaatat gaatattctt gttttaggtt ctggtggtag
     9541 agaacatgct ttaacataca aaattgtgca aagcaaattg gcaaaaaaag tatttgtagc
     9601 tcctggaaat gctggtacta acaaaattgc aaaaaacatt aatttaaatc ctttaaattt
     9661 tgaagaagtt tcaagtttca tagaaaacaa taaggtcgaa cttcttgttg ttggtcccga
     9721 agctccttta gttgctggaa ttacagattt tttaaaatca ttagacaaac acaaaaactt
     9781 attgattatt ggtcctagca aagaagctgc tatgcttgaa ggctctaaag attttgcaaa
     9841 agcttttatg caaaaatata atattcctac ggcggcacat ttttcggcaa acaaagaaaa
     9901 cttagctcaa gcaataaact ttctaaaaac tctaaaacct ccttatgttt tgaaagcaga
     9961 tggtttggcg gctggaaaag gagttttgat aattaatgaa ctaagcgaag caatttctga
    10021 attagaaaat atgcttaatg gaaaatttgg tcaagcaagt tcaaaagttg ttatcgaaga
    10081 gtttttgaaa ggaatagaat gttcggtttt tgtgcttacc gatggtaaat cttatgtaat
    10141 tttacccgaa gcaaaggatt ataaaagaat tggcgatggc gacacaggtt taaatactgg
    10201 tggtatgggt gcagtttcgc cagttccatt ttacacaaaa gaatttgccc aaaaagttaa
    10261 aaatcaaatc attactccaa ctgtaaatgg aattaaaaac gaaaattttg attattgcgg
    10321 atttattttc tttggcttaa tgaatgttga cggcaatcct tatgtaattg aatataatgt
    10381 tagaatgggc gatccagaaa cagaagctgt tctgccaaga ataaattcag attttttaac
    10441 gctacttgtg aatgctggca acaaaaattt atctaagaca aaaatagaaa tttcggagca
    10501 agttgctgta agtatagttg tggcttctgg cggctatccc gaagattatg aaaaagacaa
    10561 attaatcaca ggtattgagc ttgttagtga aagcatagtt tttcatgctg gcacaaaatt
    10621 aaatgggaat aatattgtaa gttctggagg tagagtgctt gcagtaacca gcttagcaaa
    10681 aaccattcaa gatgctgttg caaaatctta taaatcaatt gataaaatcc atttcgataa
    10741 aatgaacttt cgtaaagaca taggtcaaga cttaaaatga taaaattgtt aaaatcaaac
    10801 tcgccattaa attatttatt aatttttgga ataatgctcg ttttttgggc atttaagttt
    10861 gtatttatgc cttcgggagt agaaaattat gaagtttacg ggctatttca aataaatttc
    10921 ggacatagat taatttatca atatctaaat acttttattg gttttgtcat ttatttttta
    10981 ttagctttac ttttagtaaa agcaaattca gacctgcaaa ttgtagaaaa cacctatcaa
    11041 agtccaggaa tggtatttgc atttttaagt ggagcattta taaacataca aaagtataat
    11101 aatgtcttga tttcctgtct tttagtttct atttccatag caataatact ttattctata
    11161 aaaaaacaca ttgctttaga taatgctttt aatgctggtc ttatttttgc aattgcaaca
    11221 attttttatc cgaaaacaat agtatttttg ccaatattaa tagcaagtat gtttattgta
    11281 aaaccaactg ttgcccgcga agtcattagt ctttttcttg gaattttaac tccactaatt
    11341 ttatttttct catatttttg gctttttggt gatattgatt caataatttt gaaaactaaa
    11401 ctaagtttta ctcaagattt tatatatata agattttctt actacaataa atttattttt
    11461 gttcctctaa ttttcatttg tatttacgcc ataattgcga aatttacatt aagcgtttct
    11521 caaaaactta atacaagaaa atttcaaact ataattttaa tttactccat atattctttg
    11581 atagtatttt taattccatt catgccagac gaaactgtgg tttttcttta tccttctatt
    11641 tcgctacttt taacattagt tcttgtaaac tctaaagcaa aaatactaaa gattatatca
    11701 cttatagctt ttgcaggttt aattgtttct catttatttc agatttattt ttatttatct
    11761 attttttaaa ataattttat cacaatggcg tttagtacaa aaaaacataa attgaagata
    11821 tacagaaaaa gaaagcagtg gaagttatta ttggtattat ttgcactttt tattggtggc
    11881 ttatcaatat tttttaccaa caatttaatg aaaaagctat cggaagaaga gcataaaagg
    11941 attcaattat gggcagaagc tactgagcag ttaataaatg tatcgccaga tcaagatgtt
    12001 agctttttat ttgaagttct taaaaacaat gaaaccattc cagtgatatt agttgatatg
    12061 gacgacaata ttatttcatt taggaatatt gattccgtaa aaatgcaaaa cccagcatat
    12121 gttaaaaaga aaataactga gtttaaaaat aactctaacg aaaatttcat aataaaaacc
    12181 gaagatggcg aagctcttaa ccagatttat tattcggact cttacttact aactctattg
    12241 tattattatc catactttca attaggtttg gtatttcttt tcattttagc ttcatatttt
    12301 gctttttcgg taagcaaacg tgccgagcaa gatcaagttt ggttgggaat gtcgaaagaa
    12361 actgctcatc agcttggcac tccgatatcg tctctaatgg cgtggataga aattttaaga
    12421 gaccaagtta aagatcctga aataataaat gaactagaaa aagacacaaa acgtttagaa
    12481 aaaattgctt caagattttc taaaattggt tcaaagccta atctttccga agaaaattta
    12541 agacaaactt tgctatcaac tatcaactac atgactctta gaattcctag ttctataaaa
    12601 ataagtctag taaatccact taaagatact gatacaaaag ttaaaattaa cgaagattta
    12661 tttgaatggg taatagaaaa tttatgcaaa aatgctgttg atgcaattgg tgaaaaaggc
    12721 gaaatttcta taagctacga agaaacgcat aaacatattt atatcgacat taaagatact
    12781 ggaaaaggta ttgctaagtc ggagcaaaaa accatattta atcctggtta cacaacaaaa
    12841 gccagaggct ggggattagg cttatcattg gttaaacgca ttgttgaaca atatcatcaa
    12901 ggaaaaatat ttgttaaaag ctcagaactt ggaatcggta ctacttttag aattgtatta
    12961 aaaaaatagt tttacgataa tactctaaaa actacattta gaataatatt taaacaaact
    13021 ttatagtaat aatcattttt ttcattgtta tttgaaat
//
LOCUS       DAOPNX010000072        12978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_5602_length_12978_cov_7.880910, whole genome shotgun sequence.
ACCESSION   DAOPNX010000072 DAOPNX010000000
VERSION     DAOPNX010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12978
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5602_length_12978_cov_7.880910"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PKN53_08285"
     CDS             complement(<1..200)
                     /locus_tag="PKN53_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88984.1"
                     /translation="MKAIMIIFNHALTEKVEYMLDRLEVRGFTQWENLQGRGSVDGDP
                     HYGTHTWPEINTATIAVVEDDKV"
     gene            complement(213..3347)
                     /locus_tag="PKN53_08290"
     CDS             complement(213..3347)
                     /locus_tag="PKN53_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOD88985.1"
                     /translation="MSIYKSAVKKPITTMMVFVAIIVFGIYSLTRLPVDLYPEVELPG
                     ISVLTLYPGANSSEIEVNVSKIVENSLNAIDNLKEIHSSSRDNMSIVTIEFEWGSNLD
                     EAANDVRNSLEFIKKRLPDGAETPVIYKFNSSMMPILFFAITADENYLGLEKLLEERV
                     VNQLNRIEGVGSIGLSGMVEREIYVEVDPVKLDAFNLSVEAVGAMIQAENINMPAGSV
                     KMGDMDYNLKVEGEFIESSQIENITLGSFMGQTIKIKDVAVVKDTIKEITLDEKINGK
                     NAVSKNAVRMMVQKQSGANTVKVAKEVRKELEEIQKTLPPDVVIEPVIDSSEFISNSI
                     NNLSETLLYAFAFVVLIVLFFLGRWRATFIIVLTIPISLIVAFIFLGITGSSINIISL
                     TSLSIAIGMVVDDAIVVLENIAKHIDRGSSPREAAIYATNEVWLAVIVTTIVVVAVFM
                     PLTLVKGMTGVLFKELGWLVTITVTTSTFVAISLTPMLSSKLLKLHPPRKNRFSYDRT
                     IKVFLDKVDILYEKSIRFVLKRKKMTLLISFLIFAATMFLIPRLGTEFIPIADQSRIG
                     LVVDLQMGLRVEETQKVARQIEGIISSKYPEVRLVSTSSGADESGGISSIWWGSAGSH
                     IINMNIRLVPTSERERSCWDIGDDLRSELDKIPEINDYKVSYSGDLGMMGDNTINVEI
                     YGYDFEATDRVANEIAEKLKDLETASDVQISRDKLQPELEFVIDKEKMALYNLNTASV
                     SMALRNRVLGFTATQFREDGDEFDIVVRYNEEARNSISDIENIIVTNQQGVKVRLGDI
                     GEVKETWLPPSIDHKRRERIVTVSVVPFKTSLGDLANDIQAVISEIDKPSEILVNVAG
                     AYKDQQESFQDIGFLMLISLILVYIVMASQFESFSVPFIIMFSIPFSFSGVVLALWIT
                     GTNLSIIAALGAVLLIGIVVKNAIVLVDFINLMRDRGYAMTEAIALSGKSRLRPVLMT
                     ALTTGLGMLPLALSTGDGSEIWTPMGISVIGGLLFSTFLTLIVVPVAYAIIERPGRRR
                     KKKKAYQEEYDFMD"
     gene            complement(3364..4419)
                     /locus_tag="PKN53_08295"
     CDS             complement(3364..4419)
                     /locus_tag="PKN53_08295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD88986.1"
                     /translation="MKTIKLLLAILVAFFSILFTSCGGKADAKSEENKTEKIQPVKVT
                     LLQEEEVAREIDYSANLVAFEEVYLAPATPGRIIKVYPEIGDRVKKGQMLVQMDPTQL
                     NTSLLQLENLKKDKQRFDTLIQYGGVSKQQYDQINTQYDVTKANVELLSTNVNLTSPF
                     NGVITAKYFENGELFTGAPNTQVGKPAIVVVQQINPIKAIISVSEKYYPVVKKGMIVD
                     VKSDIYADKEFKGKISLIHPTINAQTKTFNIEIEVANNEELLRPGMFASVNMEFAKET
                     AMVVPVSAVLMQRGTNNRYVFVYKDGKAHRIDVKLGKRFDDKVEIISDEIGINDQLII
                     AGHNNLIEGDKVEIVKD"
     gene            complement(4449..5825)
                     /locus_tag="PKN53_08300"
     CDS             complement(4449..5825)
                     /locus_tag="PKN53_08300"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOD88987.1"
                     /translation="MKKTIILLSLFFYAFFAFSQETSELSFSLQQAQDYALEHNKSLK
                     NIKTDVAISKQKLWQTISQGLPQISGSVDYTNFFNYEIEFGMGGGETPNLDFTKLDAG
                     DLEILKLLQGFMGDGSTTIKMKNQSTATIQLTQLIFSGQYFTGIQIAKVGQMLAEMNI
                     EKSEQDIKESVISTYYLGLLTKKSVELLDANLENLNATLEQTQALFNAGVIEETDVEQ
                     LQMSIIMLENSKRSLLRNVELNNNILRFLLGIEGDVKIVLTESFDNIIENLDLGNLLV
                     SDFNVEENINIQMVGKQEELYEKMLCMEKWSYAPTIAGMYSHNEKLLTTDFDMNPKNM
                     FAISMSVPIFSSGLRKAKVEEKKLELYKIQNNKEIVSDQLIMQEKQMRYNLTSALEAY
                     QSQKDNIELAQKIYTKTELKFRSGVASSFDLIQANSNLLQAQNSYISASMELLQAKLQ
                     LDKLRNKI"
     gene            complement(5832..6434)
                     /locus_tag="PKN53_08305"
     CDS             complement(5832..6434)
                     /locus_tag="PKN53_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOD88988.1"
                     /translation="MDTRLRILKDAGEMFKHYGIRSVTMEDIAAELGVSKRTLYEIFS
                     DKDDLVKQVIEEEIRVHKEYCTKQIETSENVIEAIFKMGQVNVEMFRKINPLFFADLR
                     KFHPGVFRKIQKKGKLKDLSFTKSLFEAGIKDGLFSANLNIELVSSFVQKIFDIILED
                     EFKEFGRENLSNSILLPYFNGIATEKGKNLINKYLDIFKY"
     gene            6947..8389
                     /gene="asnS"
                     /locus_tag="PKN53_08310"
     CDS             6947..8389
                     /gene="asnS"
                     /locus_tag="PKN53_08310"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOD88989.1"
                     /translation="MKRIKIAALLQSNDFGKEVLVQAWVRTKRASKNVCFLALNDGST
                     INNLQVVVDITPEIEKILDRIHTGASVSVVGKTVESAGSGQALEVICKELTVLGEADP
                     DEYPLQPKRHSLEFLREIAHLRFRTNTFSAIFRIRHAMTFAIHKFFNDKGFCNIHSPI
                     ITASDAEGAGEMFQVTTLDLNNLPKTQEGKVDFTQDFFGKATNLTVSGQLEAELAALA
                     LSEVYTFGPTFRAENSNTARHLAEFWMIEPEMAFYDINDDMDLAEEMLQYLVKYALDN
                     CREDLEFLKKRREEEQKQKPQNERDEMNLIEKLEFVINNKFVRITYTEAIDILKNSKA
                     NQKGQFQYSVDKWGIDLQSEHERYLVEKHFKKPVILTDYPKEIKAFYMKLNEDNKTVR
                     AMDVLFPQIGEIIGGSQREENYDKLIGRMHEMNIPEKEMWWYLETRKFGTVPHSGFGL
                     GFERLMLFVTGMGNIRDVIPFPRTPKNAEF"
     gene            8438..9880
                     /gene="rpoN"
                     /locus_tag="PKN53_08315"
     CDS             8438..9880
                     /gene="rpoN"
                     /locus_tag="PKN53_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOD88990.1"
                     /translation="MLKQSLQQKLLQKLSPQQIQMIKLLELPTLQLEQRIKKELEENP
                     ALDEGHQEEEDIYSNEDNLDDNKNDDEFSFEDYIGDDDEIPNYRLHANNTSPDDDQKD
                     IPYSAAKSFQEHLTEQLVLKDVNEREKLLAVFIIGNIDDDGYLRRDIESIVDDIAFSQ
                     NIVATEEELYKVLSVVQDLDPAGVGAQNLQECLLLQLKRKENQSQANLNAIKIIDECF
                     DEFTKKHYSKILVKCNWDESELKEAIDLILKLNPKPGSSFGEPQTRSLMAIIPDFLLE
                     VNGSEIKISLNQRNAPELRINQSFAEMLEQYANKNSTEDKNAFLFIKQKLDSAKWFID
                     AVTQRQNTLLFTMNAIAEFQKDFFLSGDDTDLKPMILKDIAEITGLDISTISRVANSK
                     YVSTPYGNYSLKYFFSEAMQTDSGEEVSTREIKQILKELIDAENKSKPLTDEKLADEL
                     RDRGYQIARRTVAKYREQLEIPVARLRKEL"
     gene            9880..10503
                     /locus_tag="PKN53_08320"
     CDS             9880..10503
                     /locus_tag="PKN53_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD88991.1"
                     /translation="MKIKKYLEILALITCYLFHPLTIITYTIIIFLNSEHYFGFMNAN
                     LKFSIFLIFAITTLILPALFIPILYFFGLISSIQGEKKNDRLLPIFEIIIMYFFAWYF
                     MQRVAMPTILLNIVLSACICIVIAGIVTNFWKISLYTTGFGALIGFLTYFSLILNLNV
                     LQFLAYTILAAGIIGSSRLFLNRHNQPQIYFGYLLGFATVLSSMCIL"
     gene            10505..10768
                     /locus_tag="PKN53_08325"
     CDS             10505..10768
                     /locus_tag="PKN53_08325"
                     /inference="COORDINATES: protein motif:HMM:NF027444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4834 family protein"
                     /protein_id="HOD88992.1"
                     /translation="MEKFLHFIFIVLLIYFIFVVIFRFVIPFFLKLFVKRMQRKFDSQ
                     FQGQPKQEKKKHGKTYVDYIPENNNYKENSENIGEYVDFEEIE"
     gene            10765..11514
                     /locus_tag="PKN53_08330"
     CDS             10765..11514
                     /locus_tag="PKN53_08330"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOD88993.1"
                     /translation="MIKFLKKILFKVFGLSAYLKIVSASYIRLIKLGYGKKKYPELYK
                     LKDIIKPGFTCIDIGANMGYYSVFLSKYAGENGKVYAVEPVPLFCEIWKKNTKKYKNL
                     KLLNFALGNNKTVVEMGMPLFDGELHHGMTRVISDKNLDYYKKFEVEMKIPDKVFADI
                     AKIDFIKIDVEGYEHLVFENMIQTIEKHKPLIQSELSGKENRQSVISILKSLGYKTCI
                     LKNNNIVEASSKDIENHEQDFYFKPIKNEES"
     gene            11501..12922
                     /locus_tag="PKN53_08335"
     CDS             11501..12922
                     /locus_tag="PKN53_08335"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOD88994.1"
                     /translation="MKKVKIIFLIIVFALSYLNSNAHKRAKYNIVIDTDGKLEDFRTI
                     SLFTASLDFNINCITSVDGAFSPKATANNLKNLLKIYHHEGILVGEGFEKKSKKKQDI
                     NSLNFLYEVLGKTEENLPNAVDILGKSIENERKRTIVIALGPLTNISEMLQKYPNNII
                     KTEMILWYCDYENLSKDFNYTENKIAYEEILKMQVPIKFISASDKVYTQDFLSFLEEI
                     SSKHSKIFKQLTECQSVNKVEIGKEFTALYLLYPNIFEETKVGEFAINLKLQKDAEPQ
                     ILISSILNSDKPQSSVVFNEIPTEGYMVMKDIAKHSKELISRHGYNEFKITALCSEIH
                     SHIGIYNILGAKVGLRILEYLHAGLDEIKLVSYAGHQPPVSCFNDGLQVGTGSTIGYG
                     TISVDTTKQIYPAVLVSYNNRKILFKIKPEFIEMAANDISELVQKHGLESEMYWNVLR
                     QISIEKYWLNVSRYELVEIEEIE"
BASE COUNT         4561 a         2126 c         1874 g         4417 t
ORIGIN      
        1 actttatcgt cttcaaccac agctattgta gcggtattta tttcgggcca agtgtgagtt
       61 ccataatgtg ggtcgccatc tacactacct ctaccttgca agttttccca ttgagtaaaa
      121 cccctaactt ccaacctatc aagcatatac tctacttttt cggtcaaggc gtgattaaaa
      181 attatcatta ttgctttcat aatttacttt ttttaatcca taaaatcata ttcttcttgg
      241 tatgctttct ttttctttct tcttctacct ggacgctcta taattgcata tgcaacaggt
      301 acaactatca gtgttaagaa ggtagaaaac aataaacctc ctattacaga aattcccata
      361 ggtgtccaaa tttcagaacc gtctccagta ctaagagcta aaggcaacat tcccaatcct
      421 gtagttagag cagtcataag cacaggacgc aatctcgatt ttccacttaa agcaattgct
      481 tctgtcattg cataccctct atctcgcatc aagtttataa agtcaaccaa gacaatagca
      541 ttttttacaa caattccaat caataagacc gctccaagtg ctgcaataat gctaagatta
      601 gttcctgtaa tccacagagc taaaactacc cctgaaaaag aaaacggaat tgaaaacatt
      661 attataaatg gaacagaaaa cgattcaaac tgagatgcca ttacaatata aaccaaaatc
      721 aaggatatca acatcaaaaa acctatgtct tgaaacgatt cttgttggtc tttatatgct
      781 cctgcaacat taactaaaat ttcgcttggt ttatcaattt cgctaataac agcttgaata
      841 tcgtttgcta aatctcccaa agaagtttta aaaggaacaa ctgatacagt aacaatacgt
      901 tctcgtcttt tatggtctat gcttggtggt agccatgttt ctttaacttc cccaatatct
      961 cctaaacgaa ccttaacacc ttgctgattt gttactatta tgttttcaat atctgaaatt
     1021 gagtttcttg cttcttcatt atatctaaca acaatatcaa attcatcgcc gtcttcccta
     1081 aattgtgttg cagtaaaacc taaaactcta tttctcaaag ccatagaaac tgaagctgtg
     1141 tttaaattat aaagagccat tttttctttg tcgataacaa actctaattc tggctgtaat
     1201 ttatctctac ttatctgaac atctgatgct gtttctaaat cttttagttt ttcggcaatt
     1261 tcgttagcaa ctcggtctgt agcttcaaaa tcatatccat aaatttcaac attaattgta
     1321 ttgtcgccca tcattcctag atcacctgaa tatgaaactt tatagtcgtt aatttctgga
     1381 attttatcta attcgcttct taaatcgtct ccaatatccc aacacgagcg ttctctttca
     1441 cttgttggaa ctaatctgat attcatatta attatgtgag aacctgctga accccaccaa
     1501 atagaagaaa ttcctccaga ctcatctgct ccagatgatg tagaaactaa tcttacttca
     1561 ggatattttg aactaattat tccttctatt tgtcttgcaa ctttttgagt ttcttcaact
     1621 ctaagcccca tttgcaaatc aacaactaaa cctattctac tttggtcggc aataggaata
     1681 aactctgttc ccaatcttgg aataagaaac attgttgcag cgaatattaa aaatgatatt
     1741 aataaagtca tttttttgcg ttttaacaca aatctgatac ttttttcata aagtatatca
     1801 actttatcaa gaaatacttt tattgttctg tcgtaactaa aacgattttt tcttggtgga
     1861 tgtaatttca acagttttga acttaacata ggtgttaaac taatagccac aaaggttgat
     1921 gttgtaaccg taatagttac caaccagccc aattctttaa ataaaactcc tgtcattcct
     1981 tttacaagag taagtggcat aaatactgca acaaccacta tagttgtaac aataacagct
     2041 aaccacacct cattagttgc ataaattgca gcttctcgcg gacttgatcc tctgtcgata
     2101 tgttttgcaa tattttcgag aacaacaata gcatcatcta caaccatacc aattgcaata
     2161 gaaagagaag ttaatgaaat tatgtttatc gaacttcctg ttattcctaa aaatataaat
     2221 gcaacaatca atgaaattgg aatagttagt acaattataa aagttgcacg ccagcgacct
     2281 aagaaaaaca agacaattaa aacaacaaag gcaaatgcat acaacaaagt ttctgagagg
     2341 ttgttaattg agttactaat aaattctgaa gagtcaataa caggttcaat taccacatct
     2401 ggtggaagag ttttttgtat ttcttctaat tcttttctaa cttctttagc tacttttaca
     2461 gtatttgctc ccgactgttt ttgaaccatc attctaacag catttttgct aacagcattt
     2521 ttgccattaa ttttttcgtc aagcgtaatt tctttaatag tatctttaac aacagcaaca
     2581 tctttgattt ttattgtttg ccccatgaaa ctaccaagcg taatattttc tatctgtgag
     2641 ctttcaataa attcaccttc tactttaagg ttgtagtcca tgtcgcccat tttaacacta
     2701 cctgctggca tgttaatatt ttcagcctgt atcattgctc ctaccgcttc gacgcttagg
     2761 ttaaaagcgt ctaattttac tgggtcaact tccacataaa tttcgcgttc gaccatacca
     2821 gacaaaccaa tagagccaac tccttctatc ctatttagct gattaacaac cctttcctct
     2881 aacaactttt ctaagccaag ataattttcg tctgcagtaa ttgcaaaaaa caaaattggc
     2941 atcatactgg aattaaattt ataaattaca ggtgtttcgg ctccgtctgg taatcttttt
     3001 ttaataaatt ctaaagaatt tctaacatcg tttgctgcct catcaagatt tgaaccccac
     3061 tcaaactcaa ttgtaacaat cgacatatta tctcttgagc ttgaatgtat ttcttttaaa
     3121 ttgtcaattg catttagaga gttttcaaca atttttgaaa cgttaacctc aatttcactt
     3181 gagtttgccc ctggatatag agttaaaaca gaaattcctg gcaattcaac ctctggatat
     3241 aaatcaactg gcaaccttgt taacgaatat attccaaata caatgatagc cacaaaaacc
     3301 atcattgttg taattggctt ctttactgca cttttgtaaa tactcataat aaatttttaa
     3361 aaattaatct tttacaattt ctactttatc tccctcaata agattattat gtcctgcaat
     3421 tataagctgg tcgttaatac cgatttcatc agaaataatc tcaactttat catcaaatcg
     3481 ttttcctaat tttacatcta ttctatgtgc ttttccatct ttataaacaa aaacatacct
     3541 attgtttgtt cctctttgca ttaatacagc cgaaacaggg acaaccatag cagtttcttt
     3601 tgcaaattcc atatttacac ttgcgaacat tcctggtctt agaagctcct cattattagc
     3661 gacttcaatc tctatattaa aagttttagt ttgagcattt attgttgggt gaattaaact
     3721 aatttttcct ttaaattctt tatcagcata aatatccgac ttaacatcta caatcattcc
     3781 ttttttaacc acaggataat atttttccga cacacttata attgctttta ttggatttat
     3841 ttgctgaaca acaacaattg caggcttgcc cacttgagta ttaggagcac ctgtaaacaa
     3901 ttctccattt tcgaaatatt ttgctgtaat aactccatta aaaggagatg tcaaattaac
     3961 atttgtactt aaaagctcaa cattagcttt tgttacatca tattgagtat taatttggtc
     4021 gtattgttgc ttagacacac ctccatattg aattaaagtg tcgaaacgtt gtttgtcttt
     4081 tttcaaattt tctaattgta acagtgatgt gtttaattga gttggatcca tttgtaccaa
     4141 catttgtcct tttttaactc tatctcctat ttcgggataa acttttataa ttctacccgg
     4201 tgttgcaggt gctaaataaa cttcctcaaa agcaactaaa ttagccgaat aatctatttc
     4261 gcgtgcaact tcttcttctt gcaaaagagt aacttttact ggctgtattt tttcagtttt
     4321 attttcttct gattttgcat cagcttttcc gccacacgaa gtaaataaaa tactaaaaaa
     4381 tgcaactaaa atcgctaata ataattttat tgttttcatt gtttataaag tttctatatt
     4441 tttaataatt aaattttatt tctcagttta tctaattgta attttgcttg caacaactcc
     4501 attgaagcag aaatatatga attttgtgct tgcaataaat tagagtttgc ctgaattaaa
     4561 tcaaaacttg aagcaactcc acttctgaat tttaattcag ttttagtata aattttttgt
     4621 gcaagctcaa tattgtcttt ttgactttga tacgcttcca atgcagaagt taaattataa
     4681 cgcatttgtt tttcttgcat tatcaattgg tcgctaacta tttctttatt gttttgaatt
     4741 ttataaagct ctaatttttt ttcttcaact tttgctttac gtaaccctga cgaaaatatt
     4801 ggaactgaca tacttatagc aaacatattt tttggattca tatcaaaatc tgtagtaagc
     4861 aatttttcgt tatggctata cattccagca attgttggag cataagacca tttttccata
     4921 cacaacattt tttcatataa ttcctcttgc ttaccaacca tttggatatt aatattttct
     4981 tcaacattaa agtctgaaac caacaaattg cctaagtcaa ggttttcaat tatattatcg
     5041 aaagattcgg ttaaaactat tttaacgtct ccttctattc ccaaaagaaa tctcaaaata
     5101 ttattattca actcaacatt tcgtaacaaa gaacgctttg aattttctaa catgataata
     5161 gacatttgta attgctctac atctgtttct tcaataactc cggcattaaa caaggcttga
     5221 gtttgttcta aagttgcgtt caagttttct aagtttgcat ctaaaagttc aacagatttt
     5281 ttagtcaata agcctaaata ataagtgctt attactgatt ctttaatatc ttgttcagat
     5341 ttttcaatat tcatttcggc aagcatttgt ccaacttttg caatttgaat gcctgtaaaa
     5401 tactgacccg agaaaatcaa ctgagttaac tgaattgttg ctgttgactg atttttcatt
     5461 ttaatggtag tgctaccatc acccataaaa ccttgcaaaa gttttaaaat ttctaagtcg
     5521 ccagcatcaa gttttgtaaa atcaagattt ggagtttcgc caccacccat accaaattca
     5581 atttcataat taaagaagtt agtataatca acacttcccg aaatttgtgg taagccttga
     5641 gatattgttt gccataattt ttgcttagaa atagcgacat ctgttttaat attcttcaaa
     5701 cttttgttat gctctaatgc gtaatcttgt gcttgctgaa gactaaaact cagttcagat
     5761 gtttcttgac taaatgcaaa aaacgcataa aaaaacaaag acaataaaat tatagttttt
     5821 ttcattctat tctaatattt aaaaatatct aaatatttat taattaaatt tttacctttt
     5881 tctgttgcaa ttccattaaa atatggaagc aaaatagagt ttgataaatt ttctctacca
     5941 aattctttaa actcatcttc gagaataatg tcaaatattt tttgaacaaa gctacttaca
     6001 agctcaatat tcaaatttgc agaaaacaaa ccatctttaa tacctgcctc aaataaagat
     6061 tttgtaaaag ataaatcttt tagtttacct tttttctgaa tttttctaaa cacacctgga
     6121 tgaaatttgc gtaaatctgc aaaaaacaaa ggattaattt tgcgaaacat ttcaacattt
     6181 acctgcccca ttttaaaaat tgcttcaatc acattttcac ttgtttcaat ttgttttgtg
     6241 caatattctt tatgaaccct tatttcttct tctattactt gttttacaag atcatcttta
     6301 tcagaaaaaa tttcatacaa agtacgttta gaaactccaa gttctgcagc aatatcctcc
     6361 atagttacgc tacgaatacc atagtgttta aacatttcgc ctgcatcttt taatattctc
     6421 aaacgagtat ccatgaatta taatttttca agcggcaaaa ttagaaaact ttttcagtct
     6481 ccaaagtttt atatagcata ttaattaaac attaatgaaa atttaacaat actgtaactg
     6541 aactcagtta aaagttgaaa gcgatgcact gaacgtagtt gaagtgttga aagttgaaag
     6601 gggctggcgc gtctattgct tcgcagagtt cgcaagctca ctctgcttgc aatcacaaca
     6661 cttatgatgc tcgctcaaat accaatgatg ctcgctcgga tttgcaatcc gagtgcttgg
     6721 agttgtttca ctcggattac aaatccgagc gagcaaggga agtggtaggc tcagcgaagc
     6781 taaaacagag ctatatatag atttttatat tcaaaaaaca ttgccaaaaa acttaatata
     6841 gaactaataa aacaaaaaac acacaataat tatttggttt tttaatttat aaggaaaaag
     6901 atatacaata tttttatatt tttgcatttt ataaatattc taagaaatga aaagaattaa
     6961 gatagctgct ttactacaaa gcaatgattt tgggaaagaa gttttagttc aggcttgggt
     7021 tagaacaaaa agagcaagta aaaatgtttg ttttttagcc ttaaatgatg gttcaacaat
     7081 taataatttg caagttgttg ttgacattac tcccgaaata gaaaaaattt tagacagaat
     7141 acatacaggt gcttcagttt ctgttgtagg aaagactgtt gagtcggcag gtagtggaca
     7201 agctttagaa gtaatttgta aagaattaac tgttttgggc gaagcagacc ccgatgaata
     7261 tccacttcaa ccaaaacgtc atagcttaga gtttttaaga gaaattgctc atttacgttt
     7321 tcgtacaaat acattttcag caattttccg cattcgacat gctatgactt ttgctattca
     7381 taaatttttt aacgataaag gattttgcaa tattcacagc ccaattatca cagcctctga
     7441 tgcagaaggt gcaggagaaa tgtttcaggt tacaacttta gatttgaaca atcttccaaa
     7501 aacacaagaa ggcaaagttg attttacaca agattttttt ggtaaagcaa ctaacctaac
     7561 agtttcagga caattagaag cagagcttgc agctttggca ttatcagaag tttatacttt
     7621 tggtccaact tttagagcag aaaactcaaa tacagctcga catttagcag aattttggat
     7681 gatagaacca gaaatggctt tttatgatat aaacgacgat atggatttag cagaagaaat
     7741 gttgcaatat cttgtaaaat atgctttaga caattgtcgt gaagatttag aatttttgaa
     7801 aaaacgccgc gaagaagaac aaaaacaaaa gcctcaaaac gaacgtgacg aaatgaactt
     7861 aattgaaaaa ttagaatttg tcattaacaa taagtttgta agaataactt ataccgaggc
     7921 tattgatatt ttgaaaaatt caaaagcaaa ccaaaaagga caattccaat attcggttga
     7981 taaatgggga attgatttac aaagcgaaca cgaaagatat ttagttgaaa aacattttaa
     8041 aaagcctgtt attcttacag attatccaaa agaaattaaa gctttttata tgaagctaaa
     8101 cgaggataac aaaacagtaa gagctatgga cgttctgttc ccacaaatag gtgaaattat
     8161 tggtggttcg caaagagaag aaaactacga caaacttatt ggcagaatgc atgaaatgaa
     8221 tatcccagaa aaagaaatgt ggtggtattt agaaacacgg aaatttggaa cagttcctca
     8281 tagcggattt ggcttaggtt ttgaacgttt gatgctattt gttacaggaa tgggaaatat
     8341 tagagacgtt attcctttcc cacgtactcc aaaaaatgct gaattttaat tattattaaa
     8401 aaaattattg tatatttggc aataagcttt tttaattatg ttaaaacaaa gtttacagca
     8461 gaaattactt caaaagttat cgcctcagca aattcaaatg ataaaattgc tggagctacc
     8521 tactttgcaa ttagagcaaa gaataaaaaa ggaacttgaa gaaaatcctg ctcttgacga
     8581 aggacatcaa gaagaagaag acatttactc aaacgaagat aatttagacg ataataaaaa
     8641 tgatgatgaa ttttctttcg aagattatat aggagatgac gacgaaattc caaactatag
     8701 attacatgca aataacactt ctcctgacga tgaccaaaaa gatattccat attctgctgc
     8761 taaatctttt caagaacatc ttaccgaaca gcttgtttta aaagatgtta atgaaagaga
     8821 aaaactttta gcagttttta ttattggaaa tattgatgac gatggatatt tgagacgaga
     8881 tattgaaagt attgttgacg atattgcgtt ttctcaaaat attgtagcta ccgaagaaga
     8941 attatataaa gttttgtctg ttgtgcaaga cctcgaccct gctggagttg gagcacaaaa
     9001 tcttcaagaa tgtttgctat tacaattaaa aagaaaagaa aatcaaagcc aagcaaacct
     9061 aaatgcaatt aaaataattg atgaatgttt tgatgaattt actaaaaaac actattcaaa
     9121 aattcttgtt aaatgtaatt gggacgaaag cgaacttaaa gaagcaatag atttaatttt
     9181 aaaactaaat cctaagccag gttcttcgtt tggcgaacct caaactcgtt cgctaatggc
     9241 gataattcct gattttttac tcgaagttaa tggaagtgaa attaaaataa gtttaaacca
     9301 aagaaatgcc cctgaactaa ggataaatca aagttttgcc gaaatgttag aacaatatgc
     9361 taacaaaaat tctactgaag ataaaaacgc tttcttattt attaagcaaa aacttgattc
     9421 tgcaaaatgg tttatagatg ctgttactca aagacaaaac actcttttgt ttacaatgaa
     9481 tgctattgct gagtttcaaa aagatttttt ccttagtggc gatgatactg acttaaaacc
     9541 catgatttta aaagatattg cagaaattac aggtttagat atttcaacta tttcaagagt
     9601 tgctaattca aaatatgttt ctacacctta cggcaactat tcattaaaat attttttcag
     9661 cgaagcaatg caaaccgatt caggcgaaga agtatcaacc agagaaataa aacaaatact
     9721 taaagagctt attgatgcag aaaacaaatc aaaaccttta accgacgaaa aacttgcaga
     9781 cgaattaaga gatagaggtt accaaattgc aagacgaaca gttgccaagt atcgtgaaca
     9841 attagaaatt ccagttgcca ggctgagaaa agaactttaa tgaaaataaa aaaatattta
     9901 gaaatattag ctctaattac ttgttattta ttccaccctc taacaataat aacttataca
     9961 ataattatat ttctaaattc tgaacactat tttggtttta tgaatgccaa cttaaaattt
    10021 tccattttct taatattcgc aattacaaca ttaattttac cagcattatt tattcctatt
    10081 ttatatttct ttggattaat ttcatcaata caaggcgaaa agaaaaacga cagactttta
    10141 ccaatttttg aaattataat catgtatttt tttgcatggt attttatgca aagagttgca
    10201 atgccaacaa ttttattaaa tattgtttta tcagcatgca tttgtattgt tattgcagga
    10261 atagtaacaa atttttggaa aatttcactt tataccactg gctttggtgc tttaataggt
    10321 tttttaactt atttttcact aatattaaat ttgaatgttt tacaattctt agcttataca
    10381 atactcgcag ctggaataat aggctcgtca agattatttt taaacagaca taatcagcct
    10441 caaatttatt ttggatattt attaggattt gcaacagttt taagctcaat gtgtattctt
    10501 taatatggaa aagtttttac attttatatt tattgtactt ttaatatact tcatttttgt
    10561 tgttatattt agatttgtta ttccattttt tttaaaactt tttgtaaaaa gaatgcaaag
    10621 aaaatttgac tcccaatttc aaggacaacc taaacaagaa aagaaaaaac atggaaaaac
    10681 ttatgtggat tatattccag aaaacaataa ctacaaagaa aatagtgaaa atattggtga
    10741 atacgttgat tttgaagaaa tagaatgatt aaatttttaa aaaaaatatt atttaaagtt
    10801 tttgggctgt ctgcttattt aaaaattgtt tcggcaagct atataagatt gataaaatta
    10861 ggatacggca aaaagaaata tcccgaatta tataaattaa aagatattat aaagccaggt
    10921 tttacttgca tagacattgg tgcgaatatg ggttattatt ctgttttttt atcaaaatat
    10981 gcgggcgaaa atggaaaagt ttatgcagta gagccagtgc ctttgttttg cgaaatttgg
    11041 aaaaaaaaca caaaaaaata taaaaatcta aaacttttaa attttgcttt aggaaataat
    11101 aaaactgttg ttgaaatggg aatgccttta ttcgatggcg agctacatca cggaatgaca
    11161 agagttattt cagataaaaa tctcgactac tacaaaaaat ttgaagttga aatgaaaatc
    11221 cctgataaag tatttgctga cattgctaaa attgacttta taaagataga tgtagaaggc
    11281 tacgaacatt tggtttttga aaatatgata caaactatcg aaaaacacaa acctttgata
    11341 caaagcgaat tgagtggcaa agaaaacaga caatcagtaa tttcaatttt aaaaagcttg
    11401 ggatataaaa cttgtatttt aaaaaataat aatattgttg aagcaagctc taaggacata
    11461 gaaaatcacg aacaagattt ttattttaaa cctattaaaa atgaagaaag ttaaaatcat
    11521 atttttaata atagtttttg cattaagtta tctcaattca aacgcacaca aacgagcaaa
    11581 atacaatatt gtaattgata ctgacggaaa acttgaagat tttagaacta tttctttgtt
    11641 tacagcctct ttagatttta atataaattg cataacaagt gttgacggag cattttcacc
    11701 aaaggcaact gcaaataact tgaaaaatct tttaaaaatt tatcatcacg aaggtatttt
    11761 ggttggcgaa ggttttgaaa aaaaatctaa aaaaaagcaa gatattaatt ccttaaattt
    11821 tttgtacgaa gtattaggaa aaactgaaga aaacttgccc aatgcagttg atattttagg
    11881 taaatcaata gaaaatgaaa gaaaaagaac cattgttatt gctttaggtc ctttaactaa
    11941 tatttcggaa atgcttcaaa aatatcctaa caatatcatt aagacagaaa tgattttatg
    12001 gtattgtgat tatgaaaact tgtcgaaaga ttttaattat acagaaaata aaatagctta
    12061 cgaagaaatt ctaaaaatgc aggttcctat aaaatttatt tctgcaagcg acaaagttta
    12121 cacacaagat tttttaagtt ttctcgaaga aatttcttca aaacattcta aaatatttaa
    12181 acaactaaca gagtgtcaat cagttaacaa agttgaaata ggcaaagagt ttacagctct
    12241 ttatctttta tatcctaata tttttgaaga aacaaaagtt ggtgaatttg ccataaattt
    12301 aaaactgcaa aaagatgctg agcctcaaat tttgattagt tcgattttaa acagcgacaa
    12361 accacaatcc agcgtagttt ttaacgaaat tcctaccgaa ggttatatgg taatgaaaga
    12421 cattgcaaaa cacagcaaag aactaatttc acgacatgga tataatgaat ttaaaattac
    12481 tgcactttgc tcagaaattc attctcatat tggtatttac aatattttgg gggcaaaagt
    12541 tggactaaga attttggaat atctacatgc aggtttagat gaaattaaat tagtttctta
    12601 tgcaggtcat caaccgcctg taagttgttt taacgatggt ttacaagtag gaacaggctc
    12661 aacaataggt tatgggacaa taagtgtaga tactacaaag caaatttatc cggcagtttt
    12721 agtatcttat aataatcgca aaattctttt caaaataaaa cctgaattta ttgaaatggc
    12781 agccaacgac attagcgaat tagttcaaaa acatggctta gaatccgaaa tgtattggaa
    12841 tgtgttgaga caaatttcaa ttgaaaaata ttggctaaat gttagcagat atgagttggt
    12901 cgagatagag gagatagaat gattattcag ttaaaagttg aaccgagctt gcgagctcac
    12961 cgaaggtaaa gttaaaag
//
LOCUS       DAOPNX010000073        12585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_5815_length_12585_cov_5.995291, whole genome shotgun sequence.
ACCESSION   DAOPNX010000073 DAOPNX010000000
VERSION     DAOPNX010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12585
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5815_length_12585_cov_5.995291"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4504
                     /locus_tag="PKN53_08340"
     CDS             <1..4504
                     /locus_tag="PKN53_08340"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOD88995.1"
                     /translation="VSSYFKFSTLENSNLTLKVQLETEKLFGLAFYTLQNGEYVELKC
                     DTYRSTTGELKIYEEEELSSTEIIARFWVLDELGFGNVDICLSDGSLHLPPKVLQVST
                     SQFTVQELVQEVLITGCLTASNITFVGSPVQIGYFSNAIPGLDFAEGVILSTGNVMDA
                     PGPNTSGSTYTELGGAGDTQLNSLLSSGSTQDAAVLEFDFVPASDMLEFQYVFASEEY
                     PEFANSSFNDVFGFFISGGPENYNNVNVALIPGTTIPVSINNINATTNSQYYIDNQGG
                     ANIEYDAYTTTFTASKPVTACATYHIKMAIADVGDQRYDSAVFLKANSFTSGESYTVQ
                     SFNAWSSALSVMRGCTNSIEFCRTDETPLNQAVPVELSISGTAEMGVDYSEIPTEFEI
                     PAGQECVTIDFEAYDTGDEGDITIALNFENGCPCDDNVTQHIITITDAYVVTANLTND
                     GPICAGDAATLTVDVNTADMSYIGIEWSTSATGVNQITVSPTTTTTYTCDIIYPCSVL
                     TLETTVEVVEPPVVDLGPDFEVEALTTNLNAGMAPGNTGVWSVVSGPGNANVSPPNNS
                     NATATVDAFGTYAFAWTETSLAPNCVASDTINVTFFHTPTATFTVSQTPCFGDNTGIT
                     FTGEIVPSLATFQWDFGSGTIVSGTGQGPYIVNFGTPGQHTVSVTVTEADVSVVNSMN
                     VHVPYPLSATLTVNDDPCYQSCNGSASVSVTGGSPPYAYSWGSNFNYINHLCAGDYGL
                     TITDSHACEYNTQFTINEPPLLTHDSSYFHVNCYGEHSGNATVWADGGTPPYTYLWND
                     AYNGGIHDGIAAGIYTVTISDANGCTNFEQFTITQPNLLQVLTTGDFEICEHQSVNVV
                     AQELGGVAPYTFYWNNGDGTGFSEGEQTFTVVPHEDVLYTVYVVDAHDCVSNYATTDI
                     TVSPEYHLTLTVNDNTCYESCNGSAALQIQGGLAPLDFSWASDGPNLNNLCAGLYTVT
                     ITDRIGCRADTMFVVNQPPLLQLNVQTQGANCWYSENGTATAIVSGGTPPYNYIWSND
                     ERTANLTAAPGTYHLTVSDDNNCRVYGTSVITSPTKITALVLTNPTICIGGEAFVVGQ
                     ASGGTQPYYFHWEGNDGSVFEHHQFYAHPTQTTRYNLTVTDSRGCTVDGYHATVTVKP
                     PITIENIGNSINHMCEGDSAYIELDIIGGNGGPYLITDQDGQIVATPMYVKPDVTTDY
                     VFIVRDMCETPPDTARITIEVVQNPEIDFVADIFKACPGEVISFTANDTLEKADYVWN
                     FGDGVFAFVENPTHQYVDSGRFNVSLEVRDQYGCRTKHTKNQYLQILPKPYSNFSASP
                     LVAGILNPIINFENHSEGALNYFWYYGDGDSTINFATPQHIYKNIGEYEVMLVTENEF
                     NCADTAKRIILIKDEFSIYAPTAFTPNGDGINDCFRICGNGIDKNEFLLKIYDRWGEL
                     IFTTEEYDPDAYCDACGKGAWDGTRGHRDKGDKYFPNGIYYWYLWFKDYDSIGHEFSG
                     HIQLIR"
     gene            complement(4539..5441)
                     /gene="xerD"
                     /locus_tag="PKN53_08345"
     CDS             complement(4539..5441)
                     /gene="xerD"
                     /locus_tag="PKN53_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOD88996.1"
                     /translation="MNAFKNILNDYKFYLKLERNLSDNTVMAYLDDISKLVDFLEEKN
                     KTSNLKNIDSKDLHEFLVYISQKGLNEKSQARIISGLKSFFKYLIVEELIEENPTELI
                     DAPKLSRKLPDVLSIEEIELIIKSIDVSTSLGHRNRAILEVLYGCGLRVSECAELKIS
                     NIFADEEFIKVIGKGNKQRLVPIGKVALNEINNYFKSFRNHITPKKGNNDYLFLNRRG
                     SKLSRVMIFNIVKEACALAGIEKNISPHTFRHSFATHLIEGGADLRAVQEMLGHESIS
                     TTEIYTHIDREYLREAIISFHPRS"
     gene            5495..5914
                     /locus_tag="PKN53_08350"
     CDS             5495..5914
                     /locus_tag="PKN53_08350"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOD88997.1"
                     /translation="MKILIINGANLSNIGQREVEIYGKDDFLVYLESLKSEFYEFEIS
                     YFQSHVEGEIVSKLHSSNGKVDGIVLNAGAFSHTSVAIRDAIAAIKTPVVMLHISNVY
                     AREKFRANNIIAPVCRGIITGFGLLSYKLALESFKNL"
     gene            complement(5909..6676)
                     /locus_tag="PKN53_08355"
     CDS             complement(5909..6676)
                     /locus_tag="PKN53_08355"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOD88998.1"
                     /translation="MRIGICELAYIPLRSGMSHKSEMVNQLLFGELVEIQIHKEDWTK
                     VRTLHDDYEGWVDTTTLTFLDKEDKLIEEFKNAYRLHQTLEITVNDTPILLPNGAVLP
                     KNKKEFHINDNKYQIPKNFKCLSFDNKAECIIKHAHDLLNSPYLWGGRTTWGLDCSGF
                     SQYLYSLVGIQLPRDASQQINFGQTCNFISEAQAGDLAFFDDEEGNIIHVGIIYESGK
                     IIHSSGKVRIDRIDHQGIYNKNLKRYTHNLRVVKSLL"
     gene            complement(6859..7311)
                     /locus_tag="PKN53_08360"
     CDS             complement(6859..7311)
                     /locus_tag="PKN53_08360"
                     /inference="COORDINATES: protein motif:HMM:NF022838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HOD88999.1"
                     /translation="MKKLNAFIMLALILTTSLSVSCEKDKEVDIKKTPKAVQDFVSTH
                     FPNVDVLRAFKDYDDATYTWEIDLVNGFELEFNKSGEWTNIDGKTLAVPASILALLPQ
                     SIITYCTTNYPNQFIVQIEKENKTYEIKLQNNVELVFDLGGKFLRVDN"
     gene            complement(7442..9574)
                     /gene="scpA"
                     /locus_tag="PKN53_08365"
     CDS             complement(7442..9574)
                     /gene="scpA"
                     /locus_tag="PKN53_08365"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOD89000.1"
                     /translation="MKPNYKNIDIKTRAQNTVSDKSVTEKNWMTPEQIPVKKLYTADD
                     LKGMEHLNYAAGISPYLRGPYSTMYVMRPWTIRQYAGFSTAEESNAFYRRNLAAGQKG
                     LSVAFDLATHRGYDSDHPRVVGDVGKAGVAVDSILDMKILFDQIPLNQMSVSMTMNGA
                     VLPILAFYIVAAMEQGAKLEELAGTIQNDILKEFMVRNTYIYPPEFSMKIIADIFEFT
                     SQNMPKFNSISISGYHMQEAGATADIELAYTLADGLEYLRTGVKAGIDIDAFAPRLSF
                     FWALGMNHFMEIAKMRAARMLWAKIVKQFNPKNPKSLALRTHTQTSGWSLTEQDPFNN
                     VARTCIEAMGAALGHTQSLHTNALDEAIALPTDFSARIARNTQIYIQEETNICKQVDP
                     WAGSYYVESLTHEIAHKAWKLIEEIESLGGMAKAIESGIPKMRIEEAAARTQARIDSG
                     EQTIVGTNKYRLEKEDPIDILDVDNTAVREAQIKRLEQLRAERNEAECQAALAAITKC
                     VETGKGNLLALAIDAAKKRASLGEISDACEKIVGRYKAVIRTISGVYSSESKDDPDFN
                     KAKELCKTFAEKEGRQPRIMVAKMGQDGHDRGAKVVSTGYADVGFDVDIGPLFQTPEE
                     AAKQAVENDVHILGVSSLAAGHKTLIPAVIAELKKLGREDILVIAGGVIPSQDYQFLY
                     DAGVAAIFGPGTSVSKAAIAILEILLND"
     gene            complement(9589..11421)
                     /locus_tag="PKN53_08370"
     CDS             complement(9589..11421)
                     /locus_tag="PKN53_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOD89001.1"
                     /translation="MDEKLFHEFPPIPTQAWEEVILKDLKGADYEKRLIWKTLEGFSL
                     KPYYRSENLENLDYLNSNPGNFPYTRGTDYEGLKFIRQDIWVKDVNLANKQALALLNS
                     GVNSLGFIITEDLSKKDFYKLMDKINPEAAEINILACELGDKYVEMLADYAKENSYDL
                     SKIQGSNKFDPLAYMILMGKTYCSKDSCNCATNMFNKNKDILPQFSLLSVEANAYHNA
                     GANAVQELAFALSEGAEYLSIFKEQGISADEIAPKIRFNFAVGSNYFMEIAKLRAARF
                     LWAKIVEANACKDLKNAKINMHCETSQWNKTIYDPHVNMLRVTTEAMSAIIGGTDTLS
                     VIPFDFTYTEPNDFSLRIAKNVQLIITEEAHFGKVSDPAAGSYYIENLTDMIIDKTWE
                     LFLKVDEMGGYREAVKQGFIQDNINETATTRMSNVAKRREILLGTNQFPNFNEIKDEV
                     AISQKPNIENTMFKTITLHRGAVEFEKLRLKTDKAKKRPVVFMLTIGNLTFRKARAQF
                     SSNFFACAGFEVIDNNGFDNIAEGMKAAQEKNADIVVLCSSDEEYETLAPEAFEKING
                     KILVVAGNPACKDALEAKGIKNFIHVRSNVLEDLKSYQKQLGIN"
     gene            11639..12559
                     /locus_tag="PKN53_08375"
     CDS             11639..12559
                     /locus_tag="PKN53_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89002.1"
                     /translation="MGKIKLLLISVCFTISPQLVNSQFFVELNTGYAAPLYYTGKDTV
                     YNQDYIHKYRYADTIFTECGKYNMGNGFSLGSSMGYLTKIGLKFDLDIFYLNNKDINF
                     LYNNYRHEWNMAYGWNFGDSIPKYYNKYEQYIAYYSSRFSLIPKIGYVHDFKDFSLEY
                     YIGSCFSDITVYVYYNGYDESVTNTTGVVHGTFGEQIYHYYQLEESVSKFKYNKKLIT
                     PYFQLKCNYKFNPNLSLNMAASVSPFMSFNKDKGLYYYYGRKSVYDDREFLQEQELEM
                     IDIVPQGIKPNRYNLNVINFSLGIRYYFQK"
BASE COUNT         4065 a         2017 c         2075 g         4428 t
ORIGIN      
        1 agttagctct tattttaaat tttcaacttt agaaaattca aatttaactt taaaggttca
       61 gctcgaaaca gaaaaattat ttggtttagc tttttatact ttgcaaaacg gagaatatgt
      121 tgagttaaaa tgtgatactt acaggtcaac aacaggcgaa ttaaagattt atgaggaaga
      181 agagttgtca agtacagaaa ttatagctcg tttttgggtt cttgatgaat taggctttgg
      241 caatgttgat atttgtttat cagatggatc tttgcattta ccacctaaag ttttgcaagt
      301 tagtacaagt caatttactg ttcaagaact tgttcaggaa gtgttaatta ctggctgttt
      361 aactgcttct aatattactt ttgttggaag tcctgtgcaa ataggttatt tttcaaatgc
      421 aataccaggt cttgattttg ctgaaggagt gattctaagt acaggtaacg taatggatgc
      481 tccaggtcca aatacatcag gttcaactta tacagaattg ggaggagctg gtgatactca
      541 attaaattct ttattgtcta gtggtagtac acaagatgca gcagttcttg aatttgattt
      601 tgtgccagct tctgatatgc ttgagtttca atacgttttt gcaagtgaag aatatccaga
      661 gtttgcaaac tcctcattta atgatgtgtt tggttttttt attagtggag gtcctgaaaa
      721 ttataataat gttaatgtgg ctcttattcc tggcacaaca atcccagtaa gcattaacaa
      781 tataaatgct acaacgaact cacaatatta tattgataat caaggaggtg ctaatataga
      841 atatgatgct tatacaacaa cttttacagc ttcaaagccc gtaacagcat gtgctaccta
      901 tcatatcaaa atggcaatag cagatgtcgg tgaccaacgt tatgattctg ctgtgttttt
      961 aaaagcaaat tcttttactt ctggtgaatc ttatacagtt caaagtttta atgcttggtc
     1021 atctgctttg agtgtaatga gaggttgtac aaactctatt gagttctgta gaacagatga
     1081 aactccatta aatcaagcag tgccagttga gttatcaatt tctggaaccg cagaaatggg
     1141 cgtagattat tccgagatac caacagaatt tgaaattcct gcaggacaag aatgcgtaac
     1201 aattgatttt gaagcttatg atactggaga tgaaggagat attacaatag ctttaaattt
     1261 tgaaaatggt tgtccttgtg atgataacgt tactcaacat atcataacaa taactgatgc
     1321 ttatgtagtt acagcaaatt taacaaatga tggtccaatt tgtgctggag acgcagcaac
     1381 tttaacagtt gatgtaaata cagctgatat gagttatatc ggtatagaat ggagcacaag
     1441 tgctacagga gtaaatcaaa taactgtgtc accgaccact accacaactt atacttgcga
     1501 tattatttat ccttgtagtg tacttacttt agaaacaacg gtagaagtgg tagaaccacc
     1561 tgtagttgat ttaggtccag actttgaagt tgaagctttg accactaatc ttaatgctgg
     1621 tatggctcct ggaaatacag gtgtttggtc ggttgtgtct ggtccaggaa atgctaacgt
     1681 ttcaccacca aataattcaa atgcaacagc tacagttgat gcttttggaa cttatgcttt
     1741 tgcttggaca gaaactagtt tagcaccaaa ctgtgttgct tcagatacaa taaatgtaac
     1801 ttttttccat actccaacag caacattcac agtttcgcaa acaccttgtt ttggtgataa
     1861 tacgggtatt actttcacag gtgaaatagt acctagtttg gctacttttc aatgggattt
     1921 tggaagtgga actatagtta gtggaacagg tcagggtcct tatattgtaa attttggaac
     1981 tcctggacaa catacagtaa gtgttacagt tacagaagct gatgtgtctg tagttaatag
     2041 tatgaatgtg catgtgccat atccacttag tgcaacgcta acagtgaatg atgatccttg
     2101 ttatcaatct tgtaatggtt cagcaagtgt gtctgttaca ggtggttcgc ctccttatgc
     2161 ttattcttgg ggatctaatt ttaattatat taaccattta tgtgctggtg attatggttt
     2221 aacaattaca gatagtcatg cttgtgagta caatactcaa tttactatta acgaaccacc
     2281 actattaaca catgattcaa gctatttcca cgtaaattgt tatggcgaac atagcggaaa
     2341 tgcaacagtt tgggcagatg gtggaactcc tccttataca tatttgtgga atgacgctta
     2401 taatggcgga atacatgatg gaattgctgc aggaatctat acagtaacca tttcagatgc
     2461 taatggctgt actaattttg aacaatttac aattactcaa cctaatttat tacaagtttt
     2521 aacaactggt gattttgaaa tttgtgaaca tcaatctgtt aatgttgtgg cacaagagtt
     2581 gggcggagtt gctccatata ctttttattg gaataatgga gatggaacag gcttttcaga
     2641 aggagaacaa acctttactg tagtgcctca cgaagatgtt ttatatacag tttacgtagt
     2701 agatgctcat gactgtgtta gtaattatgc aacaacagat ataactgtat cacccgaata
     2761 ccacttgaca ttaaccgtaa atgataatac ttgctatgaa agttgcaatg gttctgcagc
     2821 tttacaaata caaggtggat tagcaccact agatttttct tgggcttctg atggtccaaa
     2881 tttaaacaat ctatgtgcag gactatatac tgttacaata acagatagaa ttggttgtag
     2941 agccgataca atgtttgtag ttaatcaacc tcctttgttg caattaaatg tccaaactca
     3001 aggcgctaat tgttggtatt ctgaaaatgg aactgctact gctattgtta gtggaggaac
     3061 acctccttac aattacatat ggtctaatga tgaaagaact gctaacttaa ctgcagctcc
     3121 aggtacttat catttgacag taagcgatga taataattgt agagtttatg gtacttctgt
     3181 aattacttca ccaacaaaaa taacagcttt agttttaact aatcctacaa tttgtattgg
     3241 aggagaagca tttgtagttg gacaagcatc tggcggaaca caaccatatt atttccactg
     3301 ggaaggtaat gacggaagcg tttttgaaca tcatcagttt tatgcacatc caactcaaac
     3361 aacaagatat aacctaactg taacagatag tagaggctgt actgttgatg gttatcatgc
     3421 tacagtaact gttaaaccac caataacaat tgaaaatatt ggtaatagca taaatcatat
     3481 gtgtgaaggt gatagtgctt atatagaatt ggatattatt ggaggtaatg gtggtcctta
     3541 tttaattaca gatcaagatg gacaaatagt tgctacacca atgtatgtaa aacctgacgt
     3601 tacaactgat tatgtgttta tcgttagaga tatgtgtgaa actccacctg atacagcaag
     3661 aataacaatt gaagtagtcc aaaatcctga aatagacttt gttgctgata tttttaaagc
     3721 ttgtccggga gaagttatta gctttaccgc taacgatact ctagaaaaag ctgattatgt
     3781 ctggaacttt ggagatggag tgtttgcttt tgtcgaaaat ccaactcatc aatatgttga
     3841 tagtggaaga tttaatgtta gtttagaagt tagagaccaa tatggatgta gaactaaaca
     3901 tacaaaaaat caatatttac aaatcttacc aaagccatat tcaaacttct ctgctagtcc
     3961 attagtagca ggaatattaa atcctattat taacttcgaa aaccattctg aaggagcttt
     4021 aaattatttc tggtattatg gcgatggtga ctctacaata aattttgcta ctcctcaaca
     4081 tatatataaa aatattggag agtacgaagt tatgttggta actgaaaatg agtttaattg
     4141 tgcagataca gcgaaaagaa taattttgat taaagatgag ttttctatct atgctccaac
     4201 agcgtttact cctaatggtg atggaattaa cgattgcttt agaatttgtg gaaatggtat
     4261 agataaaaat gagtttttat tgaaaattta tgatagatgg ggagaactca tttttacaac
     4321 agaagaatat gatcctgatg cctattgcga tgcttgtgga aaaggagctt gggacggaac
     4381 tagaggacat cgcgataaag gcgataaata tttccctaat ggaatttatt actggtatct
     4441 atggttcaaa gattatgatt ctataggaca tgaatttagt ggacatatcc aattaataag
     4501 ataaaaaaca gctttacact aaaaccgcat tgctaaactt aagatcttgg atgaaatgat
     4561 ataattgctt ctcttaaata ttctctatct atatgagtat aaatttctgt agtgctaata
     4621 gattcgtgtc caagcatttc ttgaacggct cgtaaatcgg caccaccttc tattaaatgt
     4681 gttgcaaatg aatgcctgaa agtatgaggt gaaatattct tttctattcc agctaaggca
     4741 caggcttctt ttacaatgtt aaaaatcata actctagata atttgctacc acggcgattt
     4801 agaaataaat aatcgttatt gccttttttg ggggtaatat gatttctaaa agatttaaaa
     4861 taattgttaa tttcgtttaa agcaactttc cctattggaa ctaatctttg cttgttgcct
     4921 ttaccaatta cttttataaa ttcttcatca gcaaaaatat tcgaaatttt tagttcagca
     4981 cattccgaaa cacgtaaacc acaaccatat aaaacttcta aaatggctct gtttctatga
     5041 ccaagacttg tacttacatc tatagatttt attatcaatt caatctcttc tattgataaa
     5101 acatcaggta attttcggct taatttgggg gcatcaatca attccgtagg attttcttct
     5161 attagttctt caactatcaa atacttaaaa aatgatttta aacccgaaat tattctagct
     5221 tgcgattttt catttaaacc tttttgtgaa atataaacta aaaactcatg caaatctttt
     5281 gaatcaatat tttttaagtt agaagtttta tttttttctt caagaaaatc gacaagtttt
     5341 gaaatatcat caagatatgc cattactgta ttgtccgata aatttctctc tagttttaaa
     5401 taaaatttat agtcgtttaa aatattttta aaagcattca ttgttataac ttaaataata
     5461 ttttatattt ttgaaaaaaa tatcttgcaa gttaatgaaa attttaataa taaacggagc
     5521 aaatcttagt aatattggac aaagagaagt agaaatttac ggaaaagatg attttttagt
     5581 ttatttagaa agtttaaaat ctgaatttta tgaatttgaa atttcatatt ttcaatctca
     5641 cgttgaggga gaaatagttt caaaacttca tagttctaat ggtaaagttg atggcatagt
     5701 tttaaatgca ggtgcttttt ctcatacttc tgttgctatt cgcgatgcta tagcagcaat
     5761 aaaaactcct gtagtgatgc ttcatatttc aaatgtttat gctagggaaa aatttagagc
     5821 aaataatata attgcacctg tttgtcgagg aataattaca ggttttggtt tgctttccta
     5881 caaattagct ttagaaagtt ttaaaaactt ataataaaga ttttaccact cttaaattgt
     5941 gagtatatct ttttaaattt ttattgtaaa tcccttgatg gtcaattctg tcaattctta
     6001 cttttcctga agaatgaata attttaccag attcataaat tattccaaca tgtattatat
     6061 ttccttcttc atcgtcaaaa aaggctaaat ctccagcttg tgcttcggaa ataaaattgc
     6121 atgtttgtcc aaaattaatt tgttgagacg catctcttgg caactggatt ccaactaaag
     6181 aatataaata ttgcgaaaat cccgaacaat ctaaacccca agttgtgcga ccaccccaca
     6241 aataaggcga atttaacaaa tcgtgagcat gtttaataat gcattcagct ttgttgtcaa
     6301 aacttaaaca tttaaagttt tttggaattt ggtatttatt atcatttatg tgaaattctt
     6361 ttttgttctt tggtaaaaca gcaccattag gaagcaggat aggagtgtcg ttgacagtaa
     6421 tctcaagtgt ttgatgcaac ctatacgcat ttttaaattc ttcgattaat ttgtcttctt
     6481 tatccaaaaa agttaaagtt gtggtatcaa cccagccttc gtaatcgtcg tgcaaagttc
     6541 taacttttgt ccaatcttct ttgtgtattt gtatttcaac caactctccg aacaacagtt
     6601 gatttaccat ttcgctcttg tgcgacattc cacttcgtag cggaatatat gctagttcac
     6661 aaattcctat acgcataatt aaagctttaa aactttttca aaattacata taaattctgt
     6721 gaataaagaa ataatttttt aacaagagaa ttttaataac tctgtctaaa gtacttgttg
     6781 aaaatttagt ttataaaaaa atcagtccaa gtttttatgc ttggactgat ctatgaacat
     6841 gtattttaga ctaactatct agttgtcaac tcttaaaaat ttaccaccta aatcaaacac
     6901 caactcaaca ttattttgca acttaatttc ataagttttg ttttcctttt caatttgaac
     6961 aataaattga tttggatagt tagtagtgca gtaggtgatg attgattgtg gcaataaagc
     7021 taaaatactc gctggtacag ctaaagtttt tccatctata tttgtccatt caccagattt
     7081 gttaaattct aattcaaatc catttacaag atcaatttcc caagtatagg tagcgtcatc
     7141 atagtcttta aaagctctaa gcacatcaac atttgggaaa tgtgtagaaa caaaatcttg
     7201 aacagcttta ggagttttct ttatgtccac ttctttatct ttttcacaac taacactaag
     7261 agaagtagta agaataagtg ctagcataat aaatgcattt agttttttca tattaatttt
     7321 actttaattt gttaaaaata taattcactc acttttttaa taatttgtaa cttttaagtt
     7381 aaaaacaaaa taagcagtca aaagtttact ttatgaactt taaaaacttt aaatttataa
     7441 gttagtcatt taacagtatt tctaaaatag caatcgcagc tttagaaacc gaagtaccag
     7501 gaccaaaaat tgcagctaca ccagcatcat aaaggaattg gtaatcttgt gaagggatta
     7561 caccgcctgc aattactaaa atatcttctc ttccaagttt tttaagttct gcaattactg
     7621 ctggaattaa agttttgtgt cctgcagcta aagatgaaac accaagtatg tgaacatcat
     7681 tttctacagc ttgttttgca gcttcttcgg gagtttggaa tagtggacct atatccacat
     7741 caaaaccaac atcggcataa cctgtagaaa ctactttagc tccgcggtcg tgtccgtctt
     7801 gtcccatttt tgcaaccatt atacgaggtt ggcgaccttc tttttcggca aaagttttac
     7861 ataattcttt tgctttatta aaatcaggat cgtcttttga ttctgaggag taaactcctg
     7921 aaattgttct tatcacggct ttataccttc ctacaatttt ttcacatgca tcagaaattt
     7981 cgcctaaact tgctcgtttt ttagcagcgt caattgctaa tgctaacaaa ttacctttac
     8041 ctgtttctac acatttggta attgcagcta aagcagcttg gcattcggct tcgtttcttt
     8101 ctgcacgtag ttgttctaat cttttaattt gagcttctct tactgctgta ttgtcaacat
     8161 caagaatatc aataggatct tctttttcta aacgatattt atttgttcct actatagttt
     8221 gttcgccact gtcgatacgt gcttgtgtgc gtgcagcagc ttcttcaatt ctcatttttg
     8281 gaattccaga ctcaatagct ttagccattc cgcctaatga ttcaatttct tcaattaatt
     8341 tccaagcttt gtgagcaatt tcatgagtta acgattcaac ataataagaa cctgcccatg
     8401 ggtcaacttg cttgcaaata tttgtttctt cttgaatata aatttgagta tttcttgcaa
     8461 tacgtgcaga aaaatctgtt ggtaaagcaa tagcttcgtc caatgcgttt gtatgtaaag
     8521 attgagtgtg tcccaaggct gcacccattg cttcgataca agttcttgca acgttgttaa
     8581 aagggtcttg ctctgtaagc gaccagcccg aagtttgagt atgagttctt agtgcaagag
     8641 attttggatt ttttggatta aattgtttta caatttttgc ccacaacatt cttgcagcac
     8701 gcattttggc aatttccata aaatggttca ttcccaatgc ccagaagaaa gacaaacgag
     8761 gagcaaaagc atcaatatca attccagctt taactcctgt gcgtaaatat tcaagtccgt
     8821 cagctaaagt atatgctaac tcaatatcag ctgtagcacc agcttcctgc atgtgatagc
     8881 cagaaatact aatgctgttg aacttaggca tattttgaga tgtaaattca aaaatatcgg
     8941 caataatttt cattgaaaat tcaggtggat aaatataagt atttctcacc ataaattctt
     9001 ttaaaatgtc gttttggata gttcctgcaa gttcttcgag ttttgctcct tgctccatag
     9061 cagcaacaat gtagaaagct aaaattggta atactgctcc gttcattgtc atagaaaccg
     9121 acatttgatt taaaggaatt tggtcgaata aaattttcat atccaaaatc gaatctactg
     9181 ccacaccagc tttcccaaca tcgcccacaa cgcgtggatg gtcagagtca taaccacggt
     9241 gagtagcaag gtcgaaagca acagacaaac ctttttgtcc ggcagctagg tttctacgat
     9301 aaaaagcatt tgattcttca gcagttgaaa aacctgcata ttgacgaatt gtccaaggac
     9361 gcataacata catagttgaa tatggaccgc gtaaatatgg cgatattcca gcagcataat
     9421 tcaaatgttc cattcctttt aaatcatcag cagtataaag tttttttact ggaatttgtt
     9481 caggagtcat ccaatttttc tcagtaacag atttgtctga aactgtattc tgagctcttg
     9541 tcttaatatc tatattttta taattaggtt tcatttttta agattttttt agttaatacc
     9601 aagttgtttt tgataagatt ttaaatcttc aagtacgtta gatctaacgt gaataaagtt
     9661 tttaattcct ttagcttcta aggcatcttt acaagctggg ttaccagcaa caactaaaat
     9721 tttgccatta attttttcaa aagcttcggg agctaaagtt tcatattctt cgtcagagct
     9781 acacaaaaca actatatcag catttttttc ttgtgcagct ttcattcctt ctgcaatatt
     9841 gtcgaaaccg ttattatcaa ttacttcaaa tcctgcacaa gcaaaaaagt tgctcgaaaa
     9901 ctgagcgcgt gcttttctga aagttaaatt tccaattgtg agcataaaca caactggtct
     9961 tttcttagct ttatcggttt ttaatcttaa tttttcaaac tctacagcac cgcgatgtaa
    10021 ggtaatggtt ttaaacattg tgttttcaat attaggcttt tgagaaatag caacttcatc
    10081 tttaatttcg ttaaagtttg gaaattggtt tgtgcctaat aaaatttcac ggcgtttagc
    10141 aacattcgac atgcgtgttg tggcagtttc gttaatatta tcttgtataa aaccttgttt
    10201 tacagcttct ctatatccgc ccatttcatc aacttttagg aatagttccc aagttttgtc
    10261 gataatcatg tcggttagat tttcgatata atatgaacct gcagctgggt cagaaacttt
    10321 gccaaaatga gcttcttcag taattattag ttgaacattt tttgcaattc ttaacgagaa
    10381 gtcgttaggt tctgtataag taaaatcaaa aggaataacg ctcaaagtat ctgttccgcc
    10441 aataattgca gacattgctt cggttgtaac tctaagcata tttacatgtg ggtcgtaaat
    10501 tgttttattc cattgtgaag tttcgcaatg catattgatt tttgcatttt tcaagtcttt
    10561 gcaagcattt gcttcaacaa tttttgccca taaaaagcga gcagctctaa gtttggcaat
    10621 ttccataaaa taatttgagc caactgcgaa attaaatcta atttttggtg caatttcgtc
    10681 agcagaaatt ccttgttctt tgaaaatgct taaatattct gctccttcgc ttaatgcgaa
    10741 agcaagttct tgcacagcat tagcaccagc attatgataa gcattggctt caacacttag
    10801 taaactaaat tgtggtaaaa tgtctttgtt tttattaaac atatttgtag cacaattgca
    10861 agaatcttta ctgcaataag ttttacccat aagaatcata taagccaatg ggtcgaattt
    10921 gtttgagcct tgaattttgc ttaaatcata agaattttct ttagcataat cagccaacat
    10981 ttcaacatat ttatcgccaa gttcgcaggc taaaatgtta atttcggcag cttcaggatt
    11041 tattttatcc attaatttgt aaaaatcctt tttgcttaaa tcttcagtaa taataaatcc
    11101 caaagaatta actccagaat ttaacaaagc aagtgcttgt ttatttgcta aatttacatc
    11161 tttaacccaa atatcttgtc taataaattt taaaccttcg taatcagtac ctcttgtgta
    11221 tggaaaattt ccgggatttg aattcaaata atccaagttt tccaaatttt cgctacgata
    11281 ataaggtttt aatgaaaatc cttctaaggt tttccagatt aatcttttct cataatctgc
    11341 accttttaaa tcttttaaaa taacttcctc ccaagcttgt gtgggtattg gaggaaattc
    11401 gtgaaacaat ttttcgtcca tatttaaaac taatttgttt ttaatttatt tgcaaaatta
    11461 ataggaataa ttgatttaca aatgatttat gtcgtaaaac ttgaatttaa tttttttaag
    11521 ttgaatattt tagttaaatt ttagattttc cacatatttt ttgctttaat tgcttttttt
    11581 attttaaatt tgtataaaat taataaatat ggatcataag cattgttata agaaaattat
    11641 ggggaaaata aaactgcttt taatctctgt ttgctttaca ataagccctc aacttgtaaa
    11701 ttctcaattt tttgttgaac taaatacagg ttatgcagcg cctttgtatt atactggaaa
    11761 agacacagtt tataatcaag actacatcca taaatataga tatgctgata ctatatttac
    11821 agaatgtgga aaatataata tgggaaatgg atttagtttg ggttcttcaa tgggatatct
    11881 aacaaaaatt gggttaaagt ttgatttaga tattttttac ttaaataata aagatattaa
    11941 ttttttatac aataactata gacatgaatg gaatatggct tacggatgga attttgggga
    12001 tagtattcca aaatattata ataaatatga acaatatatt gcatactatt ccagcagatt
    12061 ttctttaatc cccaaaattg gttatgtaca tgattttaaa gatttttctc ttgaatatta
    12121 tattggttct tgtttttctg atataactgt ttatgtatat tataatgggt atgatgaatc
    12181 agtaactaat actacaggcg tggtacacgg tactttcgga gagcaaattt accattacta
    12241 tcaattagaa gaaagtgtgt caaaatttaa gtataacaaa aaattaataa ctccatattt
    12301 tcaactaaag tgtaattata aatttaatcc aaatcttagt ttaaatatgg ctgctagtgt
    12361 ttctccattt atgtctttta ataaggacaa aggtctatat tattattacg gaagaaaaag
    12421 cgtttacgat gatagagaat ttttgcaaga acaagaatta gaaatgattg atattgtgcc
    12481 acaaggaata aaaccaaata gatataactt aaatgttata aacttttctc ttggcatacg
    12541 ttactatttc caaaaataaa attcaaaaac aattaaaaat ttagt
//
LOCUS       DAOPNX010000074        12564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_5825_length_12564_cov_6.279639, whole genome shotgun sequence.
ACCESSION   DAOPNX010000074 DAOPNX010000000
VERSION     DAOPNX010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12564
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5825_length_12564_cov_6.279639"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..1072)
                     /locus_tag="PKN53_08380"
     CDS             complement(200..1072)
                     /locus_tag="PKN53_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89003.1"
                     /translation="MKVCGFTFIRNAIKYDYPVVEAITSILPICDKFVVAVGKSEDDT
                     LNLIKNISPQKIEIIETVWDDSLREGGRVLAVETDKAFQHITIDYDWCFYIQGDEVVH
                     EKYLPTIKEAMEEHLYDERIDGLLFKYLHFYGSYDYIGESFKWYRNEIRVVRNYKNIY
                     SYRDAQGFRKEENKKLNVVSIDAYIYHYGWVKDPRTMQKKQEDFNKLWHDDQWVDKNV
                     IKADEFDYSNIDVLKKFTGTHPKVMQDRIEKINWKFDYDLSCNKMKFKDKLKLFFSKL
                     GCDIGQYKNYKLIK"
     gene            complement(1069..2145)
                     /locus_tag="PKN53_08385"
     CDS             complement(1069..2145)
                     /locus_tag="PKN53_08385"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD89004.1"
                     /translation="MRKSQNILVSPLNWGLGHATRLIPIIEWCLKNNKNVFILGNGEA
                     FKLLCERFPDCKNIYLSAPKMRYGKKTTINFHYVLSAVLLACNVFIERYKVSKIIKTY
                     NIDTIISDNRPGVFSKKVKSIYISHQINVFTRERNNLISKILTRLHKKVIKKYDVCWV
                     PDNSKSELTGRMSENVNNLNLNYIGILSRFQNVENFTAQQDLKTYEIVAIISGPETQR
                     SIFEKIIIEKLLEENRKSLIIRGLPKDSKCVENKDNIDFLNHCSDSEFFHYISNAKII
                     ICRSGYSSIMDMLVFNKNVKIIPTPGQPEQEYLAKRLNNKYNFISLQQEDLKNICLSQ
                     IDFQTKQSFENDKNKLNEFLNLHL"
     gene            complement(2145..2918)
                     /locus_tag="PKN53_08390"
     CDS             complement(2145..2918)
                     /locus_tag="PKN53_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD89005.1"
                     /translation="MATFLFDKIIFGPVKSRRLGVSLGVNLLPNDSKVCNYNCIYCEC
                     GWSDYTEKRALPKAVDVKLALDNFLSKAKKENNPPDVITFAGNGEPTLHPEFVQIIND
                     TIELRDKYFPQIKIVVLTNATNLHKPEISSALKKIEMPILKIDTMIQKDYEFLNRPHQ
                     NVKIKDIADAIIKNFKNPIIQTMFVKANTPGRNFDNTTQESLKKYFDILKKIEPSLVM
                     VYTISRATPMQGLEKCEPECLDKIAKQIETLGYETLVSY"
     gene            complement(2922..4070)
                     /locus_tag="PKN53_08395"
     CDS             complement(2922..4070)
                     /locus_tag="PKN53_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOD89006.1"
                     /translation="MRHFAANYIFDGQNLIKNAFVSVDENNTIQFISDENQALTERPF
                     MSFYNGIISPGFINSHCHLELSDNNSAENKNIGLASFIEKMVENKTKSHDLKKIKLAD
                     DIMFLKGVNLCADIVNTDLSLDVKLKSQIKYINFAEQSGLKDDIAEIRFNKIFEIFTK
                     FDESGLEVYFTPHSFYSVSEKLLEKLSEFSKNALVSIHFMESSEEKEYFENKKGKLFN
                     FLEKFHDNLLSDSSLCKIYNKLKKFEKAKSVILVHNVKTELEYLKEYKNIYFCLCPAS
                     NLLLHNELPEDNMVYSQKDKIIIGTDSLASNDSLDILKELKIMQEKYSNLSLIDLLKM
                     ATSNTSKAFDKPDFGNFTINSKPGVILIQDLDLKNLKLNKNTNIKRLI"
     gene            complement(4075..4437)
                     /locus_tag="PKN53_08400"
     CDS             complement(4075..4437)
                     /locus_tag="PKN53_08400"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HOD89007.1"
                     /translation="MNIKRFVYISVCALMLWSCAYKKTDKRFQQGENKVPKDTFELIL
                     YDVHLADAIVTSQIMKTKNNAFVDSLVYQSVFDKYGYTRQEFENTILYYVHNNMDTLH
                     SICERTVNKFNLEKGNIH"
     gene            complement(4434..5189)
                     /locus_tag="PKN53_08405"
     CDS             complement(4434..5189)
                     /locus_tag="PKN53_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD89008.1"
                     /translation="MIEVRNIDKYFGDHHVLKNVSAIFEASKINMIIGKSGSGKTVLL
                     KSIIGLHTPENGEIFFDNREFIHTKLSFKREIRKEIGMVFQGGALFTSSNVEENVMFP
                     LDLFSKMTKAEKLKRVNFCLDRVNIKDANKLYPAELSGGMQKRVAIARAIALNPKYLF
                     CDEPNSGLDPQTAILIDQLIKEITTEYGITTIINTHDMNSVMEIGDNINFIFEGRNIW
                     QGNRQDIFYSDCEELNEFVFASELYKKIREKVR"
     gene            complement(5186..5920)
                     /locus_tag="PKN53_08410"
     CDS             complement(5186..5920)
                     /locus_tag="PKN53_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD89009.1"
                     /translation="MLTALGKYVVFILSVFKRPVNFKIFFKRIVEEIEQIGIDSIGIV
                     SIVSVFMGAVLTLQVAINFVSPIIPRYLIGLTARDTFILEFSSTVVCLILAGKVGSNI
                     ASEIGTMRITEQIDALEIMGVNSANYLILPKIIAALLTFPLLSLLSTILGIVGGFLIA
                     LTTDVVTVYDYIHGLHFAFVPFYVTYSLIKMAVFSFIITSISAFFGYHTYGGALEVGK
                     SSTKAVVYSIIFVLVFNLILTRLLLT"
     gene            6022..7104
                     /locus_tag="PKN53_08415"
     CDS             6022..7104
                     /locus_tag="PKN53_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007211966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOD89010.1"
                     /translation="MNSNSNYCVIMAGGVGSRFWPISRSTMPKQFLDITGSGRTLIQQ
                     TFDRFSQIIPTENIYIVTNDEYTEIVHEQLPLVPKENILGEPMRRNTAPCIMYANFKI
                     ALKDQLANIVVSPADHLIINEEEFLQNIKDGLNYASVQNTMLTLGITPDRPATGYGYI
                     QLDGNSDEKIVKAKTFTEKPHLELAKFFVKSGDFLWNSGIFIWKTSVIDKALKEFLPE
                     QYNLFYEGISSFDTPEEKKVINRIYSAAKSISIDFGVMEKAKNVYIMKTNFGWSDLGT
                     WDSLYKLHDKDDNENAIIGDQVFVNQSFSNYINIDNKRVAILNDLEDYIVVDTDQALL
                     ICKRENEQQIGELLNEAKLRFGEDIK"
     gene            complement(7108..8424)
                     /locus_tag="PKN53_08420"
     CDS             complement(7108..8424)
                     /locus_tag="PKN53_08420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOD89011.1"
                     /translation="MHLKKHRFYLDEMADFLKQRGLRQFFYKLSYFGIDKNLPNLEIR
                     KYWLSNVVVLLTTFFLLVFVIFNLIIGNKTLAIFDIVAIFFISVPYYILQYKKCYDLN
                     KVFVVFAFIIGVIVVAFFKFDIDRQSEYLLLGGSIIPIYLFDKWKKNLLFLLFPITFF
                     AITFYFDFLEFGKIVLAPKHLILLICFLIIFVLSSHFKHSMMLYYNSLTEANATKDKL
                     FKIISHDIRNPFSSLLSSSEMQLKFLQNRDYEKLEQTSQLINTSAKQIFELTQTLLDW
                     SIAQTDSFKVKAEKLNINDLVEQVVTFCSIQAREKDIEIITNYGSPIYVELDNVMTQI
                     AVRNILINAIKFSYRNSVIRVYVRQFNDFVNIEIVDKGIGMSKQKQQEIFSKAVVTAE
                     YGTERERGSGLGIVISKELIEKQKGKISVESEEGKGSSFTLSFPLS"
     gene            complement(8455..8865)
                     /gene="tsaE"
                     /locus_tag="PKN53_08425"
     CDS             complement(8455..8865)
                     /gene="tsaE"
                     /locus_tag="PKN53_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOD89012.1"
                     /translation="MKMIVKNLDELKLAAKKFCEQIEGKRIFLFYGTMGVGKTTFIKE
                     ICKELGVKTEVTSPTFAIVNQYYGEKYKFIYHFDFYRIEKIEEIYDIGFEEYVDSEAL
                     IFIEWPQKLGQLMPEAYHEVHITELENFMREITW"
     gene            complement(8865..10424)
                     /locus_tag="PKN53_08430"
     CDS             complement(8865..10424)
                     /locus_tag="PKN53_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HOD89013.1"
                     /translation="MEQKKIRILWVDDEIDLLKPHILFLEQKGYELLTATNGSDALSL
                     VENEDLDLVFLDENMPGLSGMHTLAKIKAIYPLLPVIMITKNEEENIMDEAIGSKISD
                     YLIKPVNPNQILLTIKKHIDKNRLITEKTTNAYQSEFRKLTNEINESMTFADWIDVYR
                     KLTYWELELSEANDSTMDDVLKMQYNEANTSFSKFIKKDYLSWFDPKNSEKPLTSQNL
                     LREKVFPILKNGEKVFFLLIDNLRYDHWKSIEPLLNQYYNVVEEDLYYSILPSSTQYS
                     RNAIFSGLMPSEINKLHPDLWKNDDEEGGKNLYEEEMLENLMKRYNVKTGLHFEKVTD
                     IKFCNRISNNLNSILNNQFVVVIVNFVDALSHARTDQKMIRELANSTSAYRAITKSWF
                     NFSPLLDLLKNLAKHDVNLIISTDHGSVQVTDPVKIVGDKATTTNLRYKQGRNLNYNP
                     KEVFEILNPADAYLPKTNISSTYVFATGSDYFVYVNNFNHFVKYYKDTFQHGGISLQE
                     MIIPIVSLKPR"
     gene            complement(10441..>12564)
                     /locus_tag="PKN53_08435"
     CDS             complement(10441..>12564)
                     /locus_tag="PKN53_08435"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD89014.1"
                     /translation="EAQKDLPKKLEGEFSAVYADYYIKQKKYEEAVPKLNKAVQRTKK
                     KSLKYRYAYILAQIYENQGNLAKASELYGRVAKKSRTYEMEFNARINEAKCYVGQSKN
                     TRDIRKKLNKMLRDDKNIEYRDQIYYTIAELDQRAGHTKEAKKNYRLSSEVSVSNDYQ
                     KAISCMKLGEIYFKENDYKNAQIYYDTCMAFLPTTYDKYREIRVTSNNLNELVQYTDE
                     LELQDSLQALAKMSEKDRNKIIDDIIAEVVKQEQLEREQEQQDQINSMIFDQQRGNTM
                     NRVSAPSGGGWYFYNPAQLSFGKNEFTKKWGNRKNEDHWRRRNKSVVDFADDEDDEEL
                     ADGEEPKKNRIANNKSREYYLQDIPLTDSAMSMSHELIQEALLNMGRVYKDRFHDYVK
                     AIEAYEELNKRYANNQYLLVSYYNLYLLNKLTKNSAQEQKYKDLVIQRFPETNYAKLL
                     LNPNFVAELEQKRRQDEDLYIQVYDDFMAGKFAKVNSVATEFINENPDNVLKANFDFM
                     RVLTVGRIKEQDVFKNALVVFMQNYADHELSEAARNILEYFGTTDVDGLIADLQSRPA
                     EDYTQSELERDSVEVSKPKEQFVYNDAEEHYYVIMLNPASLDVKRLSFEIRNFNIFNF
                     SMRTFNVVNTTYDANTEFITVRSFKNQRQSVNYSKMIANSEDVFSKLANVQYKVFVIS
                     IENFNKLQKQKNINDYMLFYNENYK"
BASE COUNT         4190 a         2035 c         1563 g         4776 t
ORIGIN      
        1 acgtagttga agtgtcgaac cgagcttgcg agctcaccga aggtaaagtt gaaagaggct
       61 gacgcgtcta ttgcttcgca gagttcgctt cgctcactct gctcgctcgg attacaaatc
      121 cgagcgagcg aagttgaaag ggaaaatccg aatgatcttg atttagcgta gaagtccaag
      181 tgagtgattt tttagatttt tactttatta gcttatagtt tttatactga ccaatatcgc
      241 aacctaattt tgaaaaaaac aattttagtt tatctttaaa tttcatttta ttacaagata
      301 aatcgtagtc aaatttccaa tttatttttt caattctatc ctgcataact tttgggtgag
      361 tgcctgtaaa ttttttaagc acatctatat ttgaataatc aaattcgtcg gcttttataa
      421 catttttatc aacccattga tcgtcatgcc atagtttatt aaaatcttct tgtttttttt
      481 gcattgttct agggtctttt acccaaccat aatgataaat ataagcgtca atgctcacaa
      541 catttaattt tttattttct tcttttctaa aaccttgagc atcacgataa gaataaatat
      601 ttttataatt tctaacaacc ctaatttcgt ttctatacca cttaaaagat tcgcctatgt
      661 agtcgtaaga accataaaaa tgtaaatatt tgaataatag cccgtctatt ctctcatcgt
      721 agagatgctc ttccattgct tcttttattg ttggcaaata tttttcgtga actacttcat
      781 cgccttgaat ataaaaacac caatcataat ctattgtaat atgctgaaaa gctttgtctg
      841 tttctactgc taaaacacgt ccgccttcac gcaaactatc gtcccaaact gtttcgataa
      901 tttctatttt ttgaggcgaa atatttttta tcaaatttaa agtatcatct tctgacttcc
      961 ctactgccac aacaaactta tcgcaaatag gtaaaatcga agtaatagct tcaactacag
     1021 gataatcgta ttttattgca tttcttataa atgtaaatcc acaaactttc ataaatgtaa
     1081 atttaaaaat tcgtttaatt tatttttatc attttcaaaa ctttgtttag tttgaaaatc
     1141 aatttgcgat agacatatat ttttcaagtc ttcttgctgt aaacttataa aattatattt
     1201 gttattcaat ctttttgcta aatattcttg ctctggctgt cctggtgtgg gaataatttt
     1261 tacattttta ttaaatacca acatatccat aatggagcta tagccacttc gacaaataat
     1321 aatttttgca ttgcttatat aatgaaaaaa ctcactgtcg ctacaatgat ttaaaaagtc
     1381 aatattatct ttattttcaa cacattttga atctttaggt aatccccgaa taattaacga
     1441 cttacgattt tcctctagta atttttcaat aataattttt tcaaaaatac ttctttgagt
     1501 ttctggtccc gaaattattg caacaatttc ataagttttt aaatcttgtt gagcagtaaa
     1561 attttccaca ttttgaaatc gagaaagaat tcctatataa ttcaaattca aattatttac
     1621 attttcagac attcttcctg taagttctga ttttgaatta tctggaaccc aacatacatc
     1681 atattttttt attacttttt tatgcaatct tgttaaaatt ttacttatca aattatttct
     1741 ttcccttgta aaaacattta tttgatgaga aatataaatt gattttactt tttttgaaaa
     1801 aactcctggt ctattatcag aaattatagt atctatattg taagttttta taatttttga
     1861 aactttatac ctttcaataa aaacattaca agctaagagt actgcactta aaacataatg
     1921 aaaatttata gtagtttttt ttccatatct catttttggt gcagataaat aaatattttt
     1981 acaatcagga aacctttcgc acaacaattt aaaagcttct ccatttccta gaataaaaac
     2041 atttttatta tttttcaaac accattctat aataggaatt agcctggttg cgtgtccaag
     2101 tccccaattc aaagggctta ctaaaatatt ttggcttttt ctcatcaata acttactaaa
     2161 gtttcatatc ctaatgtctc tatttgtttt gcaattttat ctaaacattc tggctcacat
     2221 ttttcgagtc cttgcattgg tgttgctctc gaaatggtat aaaccattac caagctaggt
     2281 tctatttttt tcagaatgtc aaaatatttt ttcaaagatt cttgtgttgt gttatcaaaa
     2341 tttcgtcctg gcgtattagc tttaacaaac attgtttgaa ttattggatt tttaaaattt
     2401 ttaataattg catctgcgat atcctttatt ttcacatttt gatgtggacg atttaaaaac
     2461 tcataatctt tctgaatcat agtgtcaatc tttaaaattg gcatttcaat cttttttaaa
     2521 gctgaggaaa tttctggttt gtgcaagttt gtagcatttg taagaacaac aatttttatc
     2581 tgtggaaaat atttatcacg caattctata gtgtcattaa tgatttgtac aaactctgga
     2641 tgtaatgtag gttcgccatt tccagcaaaa gtaattacat ctggtggatt attttctttt
     2701 tttgccttag aaagaaaatt atctaaagcg agttttacat caacagcttt tggtaatgct
     2761 cttttttctg tataatctga ccagccacac tcacaatata tgcaattata attgcaaact
     2821 ttagaatcgt taggtagcaa attcactccg agcgaaactc ctaaacgtct tgattttaca
     2881 ggaccaaaaa taattttatc aaataaaaaa gttgccatat tttatatcaa tcttttaata
     2941 ttagtatttt tgtttagctt caaatttttt aaatctaaat cttgaattaa aatcacacca
     3001 ggttttgaat taattgtgaa atttccaaaa tcaggtttat caaaagcctt agacgtatta
     3061 cttgtagcca ttttaagcaa atctatcaaa ctcaaatttg aatatttttc ttgcataatt
     3121 ttcaattctt tcaaaatatc caaactatca tttgaagcaa gagaatctgt acctataatt
     3181 attttatctt tttgagaata aaccatatta tcttctggaa gttcattatg caaaagtaaa
     3241 tttgaagcag gacataaaca aaagtaaatg ttcttatatt ctttcaaata ctctaattca
     3301 gttttaacat tatgaactaa aatcacagat tttgcttttt caaacttttt aagtttatta
     3361 taaattttgc ataaactact atcactcaac aaattatcat gaaatttttc taaaaaatta
     3421 aacaatttgc cttttttatt ttcaaaatat tctttttctt cagaagattc cataaaatga
     3481 atacttacaa gagcattttt agaaaattct gagagttttt ctaataattt ttcagaaact
     3541 gaataaaaag aatgaggagt gaaataaact tctaaaccac tttcatcaaa ctttgtgaaa
     3601 atttcaaaaa ttttattgaa ccttatttct gcaatatcat cttttaagcc actttgttcg
     3661 gcaaaattta tatatttaat ttgtgattta agtttcacat cgaggcttaa atcagtattt
     3721 acaatatctg cacaaagatt aactcctttt aaaaacataa tatcgtcagc aagttttatt
     3781 ttttttaaat catgagattt tgttttattc tcaaccattt tttcaataaa agaagctaat
     3841 cctatgtttt tgttttctgc agaattatta tctgaaagtt cgagatggca atgcgaatta
     3901 ataaatccag gacttattat accattataa aaactcataa aaggtctttc tgtcaaagcc
     3961 tgattttcat cgctaataaa ttgtattgta ttattttcat caacacttac aaatgcgttt
     4021 tttatcaagt tttgtccatc aaaaatataa ttagcagcaa aatgacgcat tgtcttaatg
     4081 aatatttcct ttttctagat taaatttatt tactgttctt tcacaaatgg agtgcagagt
     4141 atccatatta ttatgaacat aatacaaaat tgtattttca aattcttgtc ttgtgtatcc
     4201 atatttgtcg aaaacacttt ggtaaaccaa agagtcaaca aaagcattgt ttttagtttt
     4261 cattatttgc gaagtaacaa tagcatctgc taaatgcaca tcgtacaaaa ttaattcaaa
     4321 agtatcttta gggactttgt tttcgccttg ttgaaatctt ttgtctgttt ttttataagc
     4381 acaagaccaa agcattaaag cacagacact tatatataca aatcgtttta tattcatcta
     4441 actttttctc taattttttt ataaagttct gatgcaaaca caaattcatt taattcttcg
     4501 caatcagaat aaaaaatatc ttgtctattt ccttgccaaa tatttctacc ttcaaaaata
     4561 aaattgatat tatctcctat ttccatcaca gagttcatat cgtgagtatt tataatagtt
     4621 gtaataccat attctgttgt tatttctttt attaattggt ctatcaaaat agcagtttga
     4681 gggtctaatc cgctatttgg ttcgtcgcaa aataaatatt ttggatttaa agcaatagct
     4741 ctagcaattg caacacgttt ttgcattcca ccacttaact cggcaggata caacttatta
     4801 gcatctttaa tatttactct atccaaacaa aagttaactc tttttagttt ttcggctttt
     4861 gtcattttag aaaacaaatc caatgggaac attacatttt cctcaacatt tgaagatgta
     4921 aataaagctc cgccttgaaa aaccattcca atttcttttc taatttctct tttaaaactc
     4981 aatttagtgt gaataaattc tctattatcg aaaaatattt caccattttc tggagtgtgt
     5041 aatccaatta tagatttaag caagacagtt ttccctgatc cacttttacc aataatcata
     5101 tttattttag atgcttcgaa aatggcactc acatttttca acacatggtg gtctccaaaa
     5161 tatttatcaa tatttcttac ttcaatcatg ttaaaagaag tctagttagt attaaattaa
     5221 aaactaaaac aaaaatgata ctgtaaacca ctgcttttgt acttgatttt ccgacttcta
     5281 atgcacctcc gtaagtatga tatccaaaaa atgcagaaat tgaagttata ataaaagaaa
     5341 agacagccat ttttataagt gaataagtaa cataaaatgg cacaaatgca aaatgcaatc
     5401 catgtatata atcataaact gttacaacat cggtagttaa agcgattaaa aaacctccta
     5461 ctattcctaa aattgtactc aacaaactaa gtaatgggaa agttagtagt gccgcaataa
     5521 tttttggtag aattaaataa ttagcagaat ttacccccat aatttccaaa gcgtctatct
     5581 gctcagttat tctcattgtt cctatttctg aagctatatt tgagccaact ttcccagcta
     5641 aaattagaca aaccacagta ctcgaaaatt ctaaaataaa agtatctcta gctgtaagtc
     5701 ctattaaata tcgcggaatt ataggtgaaa caaaattaat agcaacttgc aaagttagaa
     5761 ctgcacccat aaataccgac actatactaa ctatacctat tgaatctatt ccaatttgtt
     5821 caatttcttc tactatgcgt ttaaaaaata ttttaaaatt aactggtctt ttaaaaacgc
     5881 taagaataaa gactacatat tttcctaaag cagttaacac aataagtttt aataaatatt
     5941 ttacaaattt aattaagtat cttggatttt taaaataata atcgtatttt gtgaattatt
     6001 ttaatttaaa atttcagata tatgaattca aattctaatt attgtgtgat tatggctggt
     6061 ggtgttggga gtaggttttg gcccattagc agaagtacaa tgcctaaaca atttttggat
     6121 attacaggct caggtagaac attaattcaa caaacttttg acagattttc acaaattata
     6181 ccaactgaaa atatttacat cgttactaat gatgaatata ccgaaatagt tcacgaacaa
     6241 cttcctcttg tgcctaaaga aaatattttg ggtgaaccaa tgcgcaggaa cacagctcct
     6301 tgcattatgt atgctaattt taaaatagct ttaaaagacc aacttgcaaa tatagttgtt
     6361 agtcctgccg accatctaat cattaacgaa gaagaatttt tacaaaatat taaagatggg
     6421 ttaaattatg catcggttca aaatactatg ctaactttag gtattactcc cgaccgtcca
     6481 gcaactggat atggttatat tcagctagat ggaaatagcg atgaaaaaat tgtaaaagca
     6541 aaaactttta ccgaaaaacc tcatttggaa ttagctaaat tttttgttaa aagtggtgat
     6601 ttcttatgga actccggaat ttttatttgg aaaacatcgg ttattgataa agcattaaaa
     6661 gaatttttac cagaacaata taatctattc tacgaaggca tttcgagttt tgacacgcca
     6721 gaagagaaaa aagttataaa cagaatttat tcagctgcta aaagtatttc tattgacttt
     6781 ggagtaatgg aaaaagcaaa aaatgtttat attatgaaaa caaattttgg ctggtctgac
     6841 ttaggaactt gggattcact atataaacta cacgacaaag atgataatga aaatgctatt
     6901 attggcgatc aagtctttgt aaatcaaagt ttttcaaatt atattaatat agataataag
     6961 agagtagcta ttttaaacga tttggaagat tatattgttg ttgatacaga ccaagctttg
     7021 ctaatttgca aacgagaaaa tgagcaacaa ataggcgaat tattaaatga agcaaaactt
     7081 agatttggcg aagatataaa ataacaacta agacaaaggg aaacttagtg taaagctact
     7141 tcccttgcct tcttcacttt ctacagagat ttttcctttt tgtttttcta ttaattcttt
     7201 acttattact attcctaagc cagagcctct ctccctttct gttccatatt ctgcagtaac
     7261 aacggctttg ctaaaaattt cttgttgttt ttgtttagac attcctattc ctttgtcaac
     7321 aatctcaata tttacaaaat cattaaattg tcgtacataa actctaatta cagaattacg
     7381 atacgaaaac tttatagcgt taataagtat atttctaact gcaatttgtg tcataacatt
     7441 atccaattca acataaattg gacttccgta atttgttatt atttcaatat ctttttcacg
     7501 agcttgtatt gagcaaaaag taacaacctg ttctactaaa tcgttaatat ttaatttctc
     7561 tgctttcact ttaaaactat cagtttgggc tatagaccaa tctaataagg tttgagttaa
     7621 ttcaaaaatt tgcttagctg aggtatttat taattgacta gtttgctcta atttttcata
     7681 atctctattt tgcagaaatt ttaattgcat ttcactagaa ctcaacaaac tgctaaatgg
     7741 atttcgaata tcatgcgaaa ttattttaaa caatttatcc ttagttgcat ttgcttctgt
     7801 aagagagttg taataaagca tcatactgtg tttaaaatgc gatgacaata caaaaataat
     7861 taaaaaacaa attagcaata ttaggtgttt gggagctaaa actatttttc caaattctaa
     7921 aaagtcgaaa taaaaagtta tagcaaaaaa agttataggg aataaaagaa aaagtagatt
     7981 ttttttccat ttatcaaaca aatatatagg tataattgaa cctcctaata acaagtactc
     8041 agattgtcta tcgatatcaa atttaaaaaa cgctactact attactccaa ttataaatgc
     8101 gaaaactaca aacactttat taaggtcata gcattttttg tattgcagta tataataagg
     8161 aacagaaata aaaaaaatag caactatatc aaatattgct aatgttttat tacctattat
     8221 taaattaaaa attacaaaca ctaacaggaa aaatgttgta agcaacacga caacatttga
     8281 aagccaatat tttctaatct caagatttgg caaattttta tctattccaa aataagacag
     8341 tttataaaaa aattgtctta atcctcgttg tttgagaaaa tctgccattt cgtctaaata
     8401 aaacctatgt ttcttgagat gcaaaattat gaaaaaaaat cgaaatgcaa aattttacca
     8461 tgtaatttct ctcataaaat tttccaattc agtaatatgt acttcatgat aagcttcagg
     8521 cataagctgt ccaagttttt ggggccattc tataaatata agtgcttctg aatcaacata
     8581 ttcttcaaag ccaatatcat aaatttcttc aattttttct attctataaa aatcaaaatg
     8641 atatataaat ttatattttt cgccataata ctgattaaca attgcaaaag ttggactagt
     8701 aacttctgtt ttaacaccta gttctttaca aatttcttta ataaaagttg tttttccaac
     8761 tcccattgta ccgtaaaata aaaagatcct tttaccttca atttgctcac aaaatttttt
     8821 cgcagccaat tttaattcat ctaaattttt aactatcatc ttcattatct gggctttaga
     8881 ctaactattg gaattatcat ttcttgtaaa gatattcctc catgttgaaa agtatcctta
     8941 taatatttta caaaatgatt gaaattgttt acataaacaa agtaatcgct ccctgttgcg
     9001 aatacataag tagagctaat atttgttttt gggagataag catcagcagg atttaaaatt
     9061 tcaaaaactt cttttggatt ataatttaaa tttcttcctt gcttatacct taaatttgta
     9121 gttgttgctt tatctcctac aattttaaca gggtcagtaa cttgaacact accatgatcc
     9181 gtactaatta ttaaatttac atcgtgctta gctaaatttt tcaataagtc tagcaaaggt
     9241 gaaaaattaa accaagattt tgtaattgct ctatatgcag atgttgaatt tgccaattct
     9301 ctaatcattt tttggtcggt tcgtgcatgc gacaaagcat ctacaaaatt tacaataaca
     9361 acaacaaatt gattatttaa aatagagttt aaattatttg aaattctatt acaaaacttt
     9421 atatccgtaa ctttctcgaa atgcaagcct gttttcacat tatatctttt catcaaattt
     9481 tcgagcattt cttcttcata taaatttttg cctccttctt catcatcgtt tttccacaaa
     9541 tctggatgaa gtttatttat ttctgaaggc ataagacctg aaaaaattgc attcctagaa
     9601 tattgtgttg agcttggtaa aatagaataa tacaaatctt cttcaacaac attataatat
     9661 tgattaagca atggctctat gcttttccag tggtcgtatc ttaaattatc aattaaaaga
     9721 aagaaaactt tttctccatt ttttaaaatt ggaaaaactt tttctcgtaa taaattttgt
     9781 gaagttaaag gcttttcaga attttttggg tcaaaccaag ataaataatc tttcttgata
     9841 aatttagaaa aacttgtatt tgcttcatta tattgcattt ttaagacatc gtccatggtg
     9901 ctgtcatttg cttcactcaa ttctaattcc caataagtga gttttcgata aacgtcaatc
     9961 cagtcggcaa aggtcatgct ttcgtttatc tcatttgtca attttctaaa ttctgattgg
    10021 taagcattag tagttttttc tgtaattagc ctatttttgt ctatatgttt ttttattgta
    10081 agtaaaattt gatttggatt tactggttta ataagataat cactaatttt tgagcctata
    10141 gcctcgtcca taatattttc ttcctcgttt tttgtaatca ttattacagg caacaaggga
    10201 taaattgctt taatttttgc taaggtatgc ataccgctta agcctggcat attttcatct
    10261 aaaaagacta aatctaaatc ttcgttttcc actaatgata gtgcatcgct accatttgtt
    10321 gcagttaaaa gttcataacc tttttgttct aaaaataaaa tatgaggctt gagcaaatca
    10381 atttcatcgt ccacccaaag tattcttatc tttttttgtt ccattattta ttagctcaaa
    10441 ttatttataa ttttcattat aaaataacat ataatcatta atgtttttct gtttttgaag
    10501 tttgttaaaa ttttcgatag aaataacaaa gactttatat tgaacattag ctaattttga
    10561 aaatacatct tcactatttg caatcatttt tgaataattt acagattgcc tttgattttt
    10621 aaaagatctt acggtaataa actctgtatt agcatcgtat gttgtattta ccacattaaa
    10681 agttctcatg ctaaagttaa agatattgaa atttctaatt tcaaagctca accttttaac
    10741 atcaaggctt gcaggattta gcataattac ataataatgt tcttctgcat cgttatatac
    10801 gaattgttct tttggtttac taacttcgac actatctctt tctaattctg attgagtata
    10861 atcttcagca gggcgtgact gcaagtcagc tatcaagccg tcaacatctg tagtgccaaa
    10921 atattccaat atatttctag ctgcttctga caattcatga tcagcataat tttgcataaa
    10981 cacaaccaag gcatttttaa aaacatcttg ctctttgatt cttcccacag ttaatacacg
    11041 cataaaatca aaatttgctt ttaatacatt atctggattt tcattaataa attctgtagc
    11101 tacagaattt actttagcaa attttcccgc cataaaatcg tcataaactt gtatatataa
    11161 gtcttcgtct tgcctgcgtt tttgctcaag ctctgccaca aagttaggat ttaaaagtaa
    11221 tttagcataa tttgtttctg ggaatctttg aataactagg tctttatatt tttgctcttg
    11281 agcagaattt ttagttaatt tattcaacaa atatagatta taataagata caagtaaata
    11341 ttggttattt gcatatctct tatttaactc ttcatatgct tcaattgctt tcacataatc
    11401 atggaatcta tctttataaa ctctacccat atttagcaga gcctcttgta tcaactcatg
    11461 cgacatgctc attgcagagt cagtaagtgg aatatcttgt aaatagtatt ctctactctt
    11521 attatttgcg attcgatttt ttttaggttc ttcaccatca gccaattctt catcatcttc
    11581 gtcgtcggca aaatctacaa cagatttatt tcttcttcgc caatggtctt cattttttct
    11641 atttccccat ttcttagtaa attcattttt tccaaaactc aactgtgctg gattatagaa
    11701 gtaccaacca ccccccgaag gagcgctaac tctattcata gtattgcctc gttgttggtc
    11761 aaaaatcata gaatttattt ggtcttgctg ttcttgttct cgttctaatt gttcttgttt
    11821 tacaacttcg gcaataatat catctattat tttatttcta tctttttctg acatttttgc
    11881 caaagcttgc aagctgtctt gcaactcaag ttcatcagta tattgtacta gttcattcaa
    11941 attattagat gtaactctaa tttctcggta tttatcataa gttgttggta aaaatgccat
    12001 acaagtgtcg taataaattt gagcattttt ataatcgttt tctttaaaat aaatttctcc
    12061 caacttcatg caagaaatag ctttttgata atcattagac acactaactt ccgatgataa
    12121 tctatagttc tttttagctt cttttgtatg tccagctctt tgatccagtt ctgcaattgt
    12181 ataatatatt tggtctctat attcaatatt cttatcatct ctcaacatct tatttagttt
    12241 tttcctgatg tctctagtat ttttgctttg tcctacataa catttggctt catttattcg
    12301 agcattaaat tccatttcgt aagttctact ttttttagct acccgtccat ataattctga
    12361 agctttggct aaatttcctt gattttcata aatctgtgcc aaaatataag catatctata
    12421 tttcaaggat ttctttttag tacgctgaac agctttattc aactttggaa ctgcctcttc
    12481 gtattttttt tgttttatat aataatcagc ataaacagca gaaaattcac cttctaattt
    12541 tttgggcaaa tctttttgag cttc
//
LOCUS       DAOPNX010000075        12391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_5926_length_12391_cov_6.302286, whole genome shotgun sequence.
ACCESSION   DAOPNX010000075 DAOPNX010000000
VERSION     DAOPNX010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12391
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5926_length_12391_cov_6.302286"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..563)
                     /locus_tag="PKN53_08440"
     CDS             complement(27..563)
                     /locus_tag="PKN53_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89015.1"
                     /translation="MRKKITKLFLALWALTFIPFYGINAQQSIEITGFGTEATDTIVP
                     IHGAVFNSKQKSQMIYPAEKLKDLIGKEITKITLFTTQGNNDEWNNEIHVRMTSTTQN
                     DLKYNWSANTPTEVFVGMLVINSDNELVIELDTPFKYSGNNLLIEYDLPYVASSTSSS
                     VGFYGTTETANTNLLSII"
     gene            753..1364
                     /locus_tag="PKN53_08445"
     CDS             753..1364
                     /locus_tag="PKN53_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89016.1"
                     /translation="MNKKSFILFFFLFFALCSFSQNTETVPEATRPLTKWEKMFPQVR
                     YDKKIYKTGSNWFTAAYGLGYHINSKQYNQQNLSLAYHHRFRAMYFNAGFHYSSPEFF
                     MKRPLEKISDIHIGAGLRFEKRFFNFAFFIGPSLASSLVPKSDLVSVKHHQLGAHTEL
                     QFTFKYFYDMGIGTSIYGSFNKRYQVIGLQLHFYFSNAYVGRY"
     gene            1382..2752
                     /locus_tag="PKN53_08450"
     CDS             1382..2752
                     /locus_tag="PKN53_08450"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOD89017.1"
                     /translation="MNYINDYVEKNKERFLEELFGLIRIPSISSIQAHKPDMYKCAEY
                     WRDMILQAGADKAELMETAGNPVVYGEKIIDPKLPTVMVYAHYDVMPVDPIELWKTEP
                     FEPVIKDGKIWARGADDDKGQGFMHAKAFELMVKTNTLPCNVKFMIEGEEEIGSPSLG
                     EWCAKNKEMLKADIILVSDTSMIARDIPSITVGLRGLSYWEVEVTGPDKDLHSGLFGG
                     AVANPINVLAKMIADMQDENGRITIPGFYDDVIEASAKEREMLNKKPHCDEEYKKAIN
                     VKALKGEAGYTTTERTGIRPSFDVCGIWGGYTGEGAKTVLPSKAYAKISTRLVPNQDN
                     VKISKLFKEHFEAIAPDYVKVVVTPLHGGQGYVCPIDLPAYKAAEKAYTDVFKKVPVP
                     VRSGGSIPIISTFEEVLGIKSVLMGFGLEEDAIHSPNESYPVENFLNGIRTIPLFYKY
                     FAELMV"
     gene            complement(2805..3998)
                     /locus_tag="PKN53_08455"
     CDS             complement(2805..3998)
                     /locus_tag="PKN53_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD89018.1"
                     /translation="MKKIILNISFLLLLVSLSAQTINLDLDYYKALSYLHKNSTDSAL
                     MTINNSKSTVQNNLLKAEILYNTGDFNNAILILKKLNNNLPKETSLLLSRIYANMEFA
                     DEACFWLNEYFKYKNPIYYPELLSHREFEHINRTSEWREFWKETRYSKKEEKLSEVEY
                     LLKNENYFSAIDLLNSENFGSHEYKKQYLLAKSYFNLKDYKNANSYLNNSLKSQPKFI
                     EAIELKYELNLIENNHAEAFKQAQNLISLRPENPENLKKYSQACFLTNKFSEALYYIN
                     IYCEIFPDNLEALFLKAQIYIADKDYKNALLILNQLVVLSPGEKDFFIAKADIYYNLE
                     SWKFARDDYAMALDIDPNLPEVLFKTANCWHNMGFDDKACIYWQKAADKRHKEAARLI
                     YRYCK"
     gene            complement(3995..6583)
                     /locus_tag="PKN53_08460"
     CDS             complement(3995..6583)
                     /locus_tag="PKN53_08460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOD89019.1"
                     /translation="MKGVFLSILVILSFNLFATHNRAGEITYKQISDLKFEITIITYT
                     ATGPGWTADRPDLEIMWGDNTKSILPRVEEVFLPNYYKRNKYVGTHTYPGPGVYQIVV
                     EDPNRNFGVNNIPNSVNTMFSISTTMMINPNIGANNTPILTQAPVDKAAVGQIFIHNP
                     GAFDPDGDSLSYKLTTCRAENGEPIEGYTLPQASNSLTVNEITGDLIWDSPVYPGVYN
                     IAMLIEEWRNGIKIGSIIRDMQIEVYQTDNKPPKISADLNICIQADSLLLYTIKASDP
                     NDDIIKLTANGAPFVIENSAAKFTQTVSNPGYAEAEFEWQTQCDFIRKQPYTLNVKAE
                     DNSSPISLVDIANISISVVGPPVKNLSISSSTNKIDLNWSANYCQNAIGYNIYRKIGS
                     SGFVPKHCQVGVPESLGYTKIAFNQGYESTSFSDIKVSQGHEYCYLVTAVFNDGAEGY
                     ASEEVCARLVRGIPTITNVSVLETDETQGKIYVAWAKPTEIEIGEAPGPYQYILYRSE
                     GFYGENLTKIAEFNDINDTIFHDSLLNTKQNPYSYKVEFYNNDPANRFLIGSPHIASS
                     IFLEIEQMENALKININKNVPWVNNSYTIYRYFENTNNFDSIANSNTDTYIDKGLING
                     KEYCYFIKSIGNYSVEGIIDPIINLSQINCAEAIDTTPPCPPILQAQSICDLVHNQLT
                     WQLPDTCNNDVMYYKIFYSPTLDGKFSQIYQPKGEAREYKHFPEIGMAGCYYMTAVDS
                     FQNESPFSNKVCLDDCSYYELPNVFTPNNDGINDFFRPIEPYYFVEKIDIKIYNRWGQ
                     LMYETEDPDINWDGRNFQSGKLVNDGVYFYSCDVYEHRLTGIEIRHLNGFVHVYNSNQ
                     VNRPQE"
     gene            complement(6666..7943)
                     /locus_tag="PKN53_08465"
     CDS             complement(6666..7943)
                     /locus_tag="PKN53_08465"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOD89020.1"
                     /translation="MTEEFLHYIFKNRLWDKDHEYTTQGDVLEIISPGVHNTDSGPDF
                     FNAKIKINNKIWVGNVEIHINASDWFKHKHDKDLAYGNVILHIVFNADKAIFLKDKSE
                     IPCWEIKFPHIIYNKYAELKNLEKAIPCSDYLELVDESIKNFCLERMAIERLENKIKH
                     LEKLATKTNNNIEEIFYISLARSFGFGVNSDAFESLALSLPINILLKYNDDNLKIEAL
                     LFGQSGLLPENSDFPFVQKLISEYKHLSKIHNLKAISPVEWKKSKMRPSNFQHIRIAQ
                     FASLIPVFKDLVYSITNLELCKNTSNLLKPNVSDFWKNHYNFDKETSKTNSNFSKTAQ
                     NIISINTLIPFAFFYFSKYSCENIEDKVIDWLQNIKPENNKETREWEKVGFLNKNSLD
                     SQALLHLKREYCNSHNCHKCAIGLEIMKQLSQL"
     gene            complement(8171..8638)
                     /locus_tag="PKN53_08470"
     CDS             complement(8171..8638)
                     /locus_tag="PKN53_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89021.1"
                     /translation="MKNILSIILVAALGSLIAFTSCKKKDTYEEVELKTVVISGTVYA
                     DLDATSIGLEKVGAGTKIIFRIDSRDLVQAPVGTHVYEILQYETTTKDEGKYEISLPT
                     VKFNNVTVDIVPVNFEKAQKTGADTYKTTVYTGNAVQITTSEGERYYQDLTYF"
     gene            complement(8681..9526)
                     /locus_tag="PKN53_08475"
     CDS             complement(8681..9526)
                     /locus_tag="PKN53_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89022.1"
                     /translation="MKHLTIFICAIFLVSGLFAQEVDMKSKRGENILPKAGEFALGID
                     AMPVVDYFGNLFNGTMGNSGAFNFIPTINRTALTIYGKYYLDDQVAIRGSVRLGHTNL
                     YDREFVTKNMTIPNPDAKVTDVMHVNATNIVLGGDYLMYRGKGRVQGYYGGGAFFNYN
                     SRNVNYKYGNEMTAEFTSPSYYDFTAGAEVAGSQRILSVHNNDNLGFSLRGVVGVEYF
                     FAPKISIGGEMGLGFNISKEGGFVTTETWTGTGREVKTEEIAIDSFVGIDTFTSGSLF
                     IMFHF"
     gene            complement(9759..9917)
                     /gene="rpmH"
                     /locus_tag="PKN53_08480"
     CDS             complement(9759..9917)
                     /gene="rpmH"
                     /locus_tag="PKN53_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOD89023.1"
                     /translation="MKRTFQPSNRKRKNKHGFRERMATVGGRKILAARRAKGRKKLTV
                     SDEPRHKR"
     gene            complement(9976..10629)
                     /gene="rsmG"
                     /locus_tag="PKN53_08485"
     CDS             complement(9976..10629)
                     /gene="rsmG"
                     /locus_tag="PKN53_08485"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007133836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOD89024.1"
                     /translation="MQEVLLKYFPQINQTQLKQFEQLEIIFPEWNNKINCISRKDIDN
                     LFVNHILHSLSIAVKFNFDKTHSIADIGTGGGFPGIPLAIMFPQTKFDLIDSIAKKIS
                     VVSAIKEQLDLQNITAINARAETLMPKYDFVVSRAVTAFPKFYNIAKNIILSKTEHNK
                     QGIIYLKGGNFDEELKNFKNFQIYNISELFDYEYFETKKIIYLPISQKKQQKFLNKK"
     gene            complement(10634..11227)
                     /locus_tag="PKN53_08490"
     CDS             complement(10634..11227)
                     /locus_tag="PKN53_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD89025.1"
                     /translation="MVERSNFSAKASKDLEIVEQALSGNEKAFAQLLDRYQDPIYFLL
                     LKKIGNENDAEDLTMETFAKAFNNLDKYTPDYAFSTWLFRIAINNCIDFVRKVKVRPQ
                     SIDSTSEYHNSDNPNFTSPNPNPEDLLIKKQKSKDLKSVIKTLKPRYATLIELRYYQE
                     FTYEEIAEELNLPIGTVKAQLYRARELLAPLLTNKYK"
     gene            complement(11221..12327)
                     /locus_tag="PKN53_08495"
     CDS             complement(11221..12327)
                     /locus_tag="PKN53_08495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD89026.1"
                     /translation="MSIFLIIIFIVFCISTSVQLFYYLYLFPSVIFKKEKLSASTNDE
                     PVSIIIAAKNEAENLEKNLPKILNQNFNKFEIIVVLDQCTDNSLEVLELIQEENKILK
                     ILQTQTQNIGKKHALSLGIENAKHEKLVFIDADCYPAHNNWLRHICSKFSANKKIVLG
                     LGMFELENNLLNAFIRYDAQLIALTYMSSAKKLKPYMGVGRNLAYSKKLWIENSGFES
                     HLDIASGDDDLFIIEVATKQNTAICTHHESISLSKTKETYKAFVKQKTRHLSTSKKYN
                     LYEKIISGSELISRFLFLISAIILLSNNAFLLITSIILILRILIISVILKKFSVKIKN
                     KLLIFYIIIFDNFAIIFYFQILIQKKFKADKNQW"
BASE COUNT         3957 a         1785 c         1935 g         4714 t
ORIGIN      
        1 taatgttttt ttgcaaagat aacaaattat ataatagaaa gcaaattagt attagcagtt
       61 tctgtagttc catagaaacc gacagaactc gaagtagagc ttgcaacata aggtaagtca
      121 tactctatca acaaattgtt gccactgtat ttgaaaggtg tgtctaactc aataactaat
      181 tcattatcag agttgataac caacattcct acaaatactt ctgttggcgt gtttgcactc
      241 cagttatatt taagatcgtt ttgtgttgtg cttgtcattc taacatgtat ctcattgttc
      301 cactcatcat tgttgccctg agttgtaaat aaagtaattt ttgtaatttc tttacctatt
      361 aagtctttta acttttcagc aggataaatc atctggcttt tctgtttact gttaaaaacg
      421 gctccatgta taggaactat tgtatctgtt gcttcagttc caaatcctgt aatttctata
      481 ctttgttgtg cattaattcc gtaaaatgga ataaacgtta gtgcccatag tgctaaaaat
      541 aattttgtaa ttttttttct catatcaatg ttaattttaa ataaaatatt aaaatgcaaa
      601 tgtacaattt taaatgcaaa ttttgtacca ataaaaggta tttgaaagca tgtaataaag
      661 ctttaaaaat cataaaatta tttttattaa tcatataaag taatttattg tgtgaatttt
      721 cgttaaaaac tttatctttg ttgtttgaat ttatgaataa aaagagtttt atattatttt
      781 tcttcttatt ttttgctttg tgttcatttt cacaaaacac agaaacagta ccggaggcaa
      841 cacgtccatt aacaaaatgg gaaaaaatgt ttcctcaagt tagatatgat aaaaaaatat
      901 ataaaactgg aagtaattgg tttacagcag cttatggttt aggctatcat attaattcaa
      961 aacaatataa tcagcaaaat ttatcattgg cttatcatca tcgttttcga gctatgtatt
     1021 ttaatgcagg ttttcattat tcaagtcctg aattttttat gaaacgtcca ttagaaaaaa
     1081 ttagtgatat tcatattgga gcaggcttac gtttcgaaaa acgttttttt aattttgctt
     1141 tttttattgg tccctcgctt gcatcatcat tagtgccaaa aagtgatctc gtttcagtaa
     1201 aacatcatca gttgggtgcg catacagagt tgcaatttac tttcaaatat ttttatgata
     1261 tgggaatagg gacatctatt tatggaagtt ttaataaacg ctatcaagta attggtttac
     1321 aattacattt ttatttttca aatgcttatg ttggacgtta ttaattataa aatttaaaat
     1381 tatgaattac attaatgatt atgtagaaaa aaacaaagaa agatttttag aagagctttt
     1441 tggtcttatc cgaattcctt ctattagttc aattcaagct cataagcctg atatgtacaa
     1501 atgtgcagaa tactggcgtg atatgatttt acaagctggt gcagataaag ccgagcttat
     1561 ggaaacagca ggaaatccag ttgtttatgg agaaaaaata attgatccaa aattgccaac
     1621 agtaatggtt tatgctcatt atgatgttat gcctgttgat ccaatagaac tttggaaaac
     1681 tgaacctttt gagcctgtaa taaaagacgg taaaatttgg gctcgcggtg ctgacgacga
     1741 taaaggtcaa ggttttatgc acgcaaaagc atttgagcta atggtaaaaa caaatacttt
     1801 gccttgtaat gtaaagttta tgattgaagg tgaagaagaa attggttctc caagtcttgg
     1861 cgaatggtgt gcaaagaata aggaaatgct taaagcagat attattttag tttcggatac
     1921 tagtatgatt gctcgcgata ttccaagtat tacagttggt ttgcgtggtt tgtcatattg
     1981 ggaagtagaa gttacaggtc cagataaaga tttacatagt ggattgtttg gtggtgctgt
     2041 tgcaaatcca ataaatgtgc ttgcaaaaat gattgcagac atgcaagacg aaaatggcag
     2101 aattacaatt ccaggttttt atgatgacgt tatcgaagct tctgcaaaag aacgcgaaat
     2161 gttaaataaa aaacctcatt gtgatgaaga atataaaaaa gcaattaatg taaaagcatt
     2221 gaaaggcgaa gcaggttaca caactactga acgtacaggt attcgtccaa gttttgatgt
     2281 ttgtggaatt tggggtggtt atactggcga aggagcaaaa acagttcttc catcaaaagc
     2341 ttatgcaaaa atttctactc gtttagttcc aaatcaagat aacgtaaaaa tttcaaaact
     2401 ttttaaagaa catttcgaag ctattgcacc agattatgtg aaagtggtag taactccttt
     2461 acatggcggt caaggctatg tttgtcctat tgatttacca gcttataaag cagccgagaa
     2521 agcttacaca gatgtattta aaaaagttcc agttccagtt cgtagcggtg gttctattcc
     2581 aattatttct actttcgaag aagtgttagg aattaaatct gtgcttatgg ggtttggttt
     2641 agaagaagat gctattcact cgccaaacga aagctatcct gtggaaaatt tcttaaatgg
     2701 tattagaact attccattgt tttataaata ttttgccgag ttaatggtat gataaaatat
     2761 atgcgtagta gaaccatcgc atgcgatggt tctacatgca tatattattt acaatatcta
     2821 taaattaatc ttgcagcctc tttgtgtctt ttgtctgcag ctttctgcca ataaatgcag
     2881 gctttgtcgt caaaccccat gttgtgccaa cagtttgcag ttttaaacaa aacttcgggt
     2941 aggttagggt caatgtctaa agccatagca taatcgtctc tggcaaattt ccacgactct
     3001 aaattatagt aaatatcagc ttttgcaata aaaaaatctt tttctccagg acttaataca
     3061 actagctgat ttaaaataag taaagcattt ttataatctt tatctgctat ataaatttgt
     3121 gcctttaaga ataaagcttc taaattatct ggaaaaattt cgcagtatat attaatatag
     3181 tatagtgctt cgctaaattt atttgtaaga aaacaagctt gtgaatattt ttttaaattt
     3241 tcaggatttt caggtcttag actaattaag ttttgggctt gtttaaaagc ttctgcatgg
     3301 ttgttttcaa ttaaatttag ttcatattta agttcaatag cttcaataaa tttgggctga
     3361 gattttaaag aattattcaa ataagaatta gcatttttat aatctttcaa attaaaatat
     3421 gattttgcta aaagatattg ctttttatac tcatgagaac caaaattttc gctatttaaa
     3481 aggtctattg cagaaaaata attttcattt tttaacaaat attctacttc ggaaagtttt
     3541 tcttcttttt tagaatatcg agtttctttc caaaattctc tccattcaga agttctatta
     3601 atgtgttcaa attctcgatg tgaaagcaat tcaggataat aaattgggtt tttatattta
     3661 aaatattcat ttaaccaaaa acatgcttca tctgcaaatt ccatattagc ataaattcgc
     3721 gataaaagta atgaggtttc tttaggcaag ttattattca attttttcaa aattaaaatt
     3781 gcattattaa aatctcctgt gttgtaaaga atttctgctt ttaataaatt attttgaact
     3841 gtagattttg aattatttat tgtcattaaa gcagaatctg tggagttttt atgtaaatat
     3901 gaaagtgctt tgtaataatc taaatctaag ttaatagttt gagccgaaag actaactagc
     3961 aaaagcaaaa aactgatatt taaaataatt tttttcattc ttgcggtcta ttaacttggt
     4021 tactattata aacatgcaca aatccattca aatgtctgat ttctatgcca gttaacctat
     4081 gttcatacac atcacagcta tagaaatata ctccatcgtt tactaatttt ccactttgga
     4141 aatttcttcc gtcccaattt atgtcggggt cttcggtttc gtacattaat tgtccccatc
     4201 tgttataaat tttgatgtca attttttcaa caaaatagta tggctctata ggtctgaaaa
     4261 agtcatttat tccatcatta tttggagtaa aaacatttgg aagttcataa tatgaacaat
     4321 catctaagca tactttattt gaaaaaggac tttcgttttg aaaagaatca acagcagtca
     4381 tataataaca gccagccatt ccaatttctg gaaaatgttt gtattctcta gcttcgcctt
     4441 taggttgata gatttgagaa aatttaccat ctagagtagg ggagtagaaa attttataat
     4501 acattacatc gttgttgcaa gtatcgggta actgccatgt tagctggttg tgaactaaat
     4561 cgcaaattga ttgtgcttgt aaaatcggtg ggcaaggcgg agttgtgtca atggcttcgg
     4621 cacaatttat ttgagataaa ttaattattg ggtcaataat tccttcgaca gaatagttgc
     4681 caatactttt tatgaaatag caatattctt ttccatttat taaacctttg tctatatatg
     4741 tgtctgtgtt tgaatttgca atagaatcaa agttgtttgt gttttcaaaa tatctataaa
     4801 tagtgtaaga attatttacc caaggtacat ttttgttaat atttattttt aaagcatttt
     4861 ccatttgttc aatttctaaa aatattgatg atgctatgtg tggcgaacct attaaaaatc
     4921 tgtttgctgg gtcgttattg taaaattcaa ctttgtatga atatggattt tgctttgtat
     4981 ttaatagcga atcatgaaat attgtatcgt taatgtcgtt aaattcagca atttttgtaa
     5041 ggttttcacc gtaaaatcct tctgagcgat ataaaatata ttgataagga cctggtgctt
     5101 cgccaatttc aatttcggtt ggttttgccc aagcaacata tatttttcct tgagtttcgt
     5161 cagtttctaa aacactaaca tttgtgatag ttggaatacc tcttactaat ctagcacaaa
     5221 cttcttctga tgcgtatcct tcagctccgt cattaaaaac tgctgttaca agatagcaat
     5281 attcatgtcc ttgcgaaact tttatatcag aaaatgaagt tgattcatat ccttggttaa
     5341 aagcaatttt tgtatatcct aaactttcag gaactccgac ttggcagtgt ttaggaacaa
     5401 atcctgagct tccaattttt ctataaatat tataacctat tgcattttga caatagtttg
     5461 cactccaatt caggtctatt ttatttgtgg aactcgaaat actcaaattt tttactggtg
     5521 gtcctacaac actaatgcta atatttgcaa tgtcaactaa actaatagga gaactgttgt
     5581 cttcggcttt tacatttaaa gtatatggct gttttctaat aaagtcgcat tgtgtttgcc
     5641 attcaaattc tgcttcggca tatcctgggt tggaaacagt ttgtgtaaat tttgcagcag
     5701 aattttctat aacaaatgga gctccgtttg cagtgagttt tataatatcg tcatttgggt
     5761 cgcttgcttt aatagtatat agtaaaagcg aatctgcttg aatacaaata tttaggtcag
     5821 cagaaatttt tggtggtttg ttgtctgttt gataaacttc aatttgcata tctcttatta
     5881 tcgaaccaat ttttattcca tttctccatt cttcaatgag catagcaata ttataaactc
     5941 ctggatatac tggtgaatcc caaattaaat ctcctgtaat ttcgtttact gtaagtgaat
     6001 ttgacgcttg tggaagagta tagccttcta ttggttcgcc gttttcggct ctgcaagttg
     6061 tgagtttata tgatagactg tcgccgtcag ggtcaaaagc tccaggatta tgaataaaaa
     6121 tttgtccaac agcagctttg tcaacaggtg cttgagttaa aataggagtg ttgtttgctc
     6181 ctatgttagg gtttatcatc attgtggttg agatggaaaa catggtgttt acagagtttg
     6241 gtatgttgtt tactccaaaa tttctattag gatcttcaac aacaatttgg taaactcctg
     6301 gacctggata tgtatgagtt ccaacatatt tgtttctttt gtaataattt ggtaagaaaa
     6361 cttcctcaac tcttggtaag atgcttttag tattatctcc ccacataatt tctaagtctg
     6421 gtctgtcggc agtccaacca ggaccagttg ctgtgtaggt tataattgtt atctcaaatt
     6481 ttaaatcaga aatttgttta taagtaatct cgccagcacg attatgtgta gcaaataagt
     6541 taaaacttaa aattactaat attgacaaaa aaacaccttt cataatttac aaagttatga
     6601 atttttattt taagatgaat tgttttaaga aaatttttac aaaactgttg aaattagggt
     6661 ctattttata gttgacttaa ttgtttcata atttctaaac ctattgcaca tttgtggcaa
     6721 ttgtgcgaat tgcaatattc tcgttttaag tgtaaaagtg cttgagaatc aagtgagttt
     6781 ttgtttaaaa aaccaacttt ctcccattct ctagtttctt tattgttttc aggttttata
     6841 ttttgaagcc agtcaataac tttatcttca atattttcgc aagaatattt gctaaaataa
     6901 aaaaatgcaa atgggattaa agtatttatg ctgatgatat tttgggcagt tttgctaaaa
     6961 tttgaatttg ttttgcttgt ttctttatca aaattatagt ggtttttcca aaaatccgaa
     7021 acatttggtt ttaataagtt tgaagtgttt ttgcataatt ctaaatttgt aatgctataa
     7081 actaagtctt taaatactgg aattaaactt gcaaattgtg ctattctgat atgttgaaaa
     7141 tttgaaggtc gcatttttga ttttttccat tctacaggac ttattgcttt taaattatga
     7201 attttcgaaa gatgtttgta ttccgagatt aatttttgta caaatgggaa atccgaattt
     7261 tctggtaata atcctgattg tccaaagaga agggcttcta ttttaaggtt atcgtcatta
     7321 tattttagta gaatgttgat aggtaatgat aaagctaaac tctcaaaagc atctgaattt
     7381 actccaaagc caaagctacg agctaagcta atataaaaaa tctcttcaat attattattt
     7441 gtctttgtgg caagtttttc taaatgctta attttgtttt caagtctttc tatagccatt
     7501 ctttctaagc agaaattttt tatactttca tcaactaatt ctaagtagtc tgaacaggga
     7561 atagcttttt ctaaattttt gagttcagca tatttattat aaattatatg tggaaatttt
     7621 atttcccagc aaggaatttc gcttttatct tttagaaaaa tagctttatc agcattaaaa
     7681 actatatgta aaattacatt gccgtaagct aaatctttat catgtttgtg tttaaaccaa
     7741 tctgatgcat ttatatgaat ttctacattt ccaacccaaa ttttgttgtt aattttaatt
     7801 ttagcattaa aaaaatcagg accagaatct gtattatgta ctcctggact aattatttct
     7861 aaaacatcgc cttgagttgt atattcatgg tctttatccc aaagtcgatt tttaaaaata
     7921 taatgtaaaa attcttctgt catggttttt taattttaac tgatttgata gtgattgagt
     7981 gtacggtgtt tcggctccgc tcaactaccg taaacttaac caatacaaca ctactagctc
     8041 gctcattgct ctcgctcatt gagcgaagtc gaaatgagcg aaatagttgg acattattga
     8101 gacacattag taaataaaaa atgcccccta tatgagagca tttttttata atatgttttt
     8161 tatttatcta ctagaaatag gttaaatctt ggtaatatct ttcaccttct gaagtagtga
     8221 tttgaactgc attgccagta tatactgtag ttttgtatgt atcagcacct gttttttgag
     8281 ctttttcaaa attcacagga actatgtcaa cagttacatt gttaaatttt acagttggta
     8341 aagagatttc atatttacct tcgtctttag tagtagtttc atattgtaag atttcgtaaa
     8401 catgagtacc aacaggagct tgtactaaat ctcttgagtc aatgcggaaa atgatttttg
     8461 tgccagcacc aactttttca aggccaatac ttgttgcgtc aaggtcggca taaacagttc
     8521 ctgaaataac aacagttttc aactctactt cttcgtaagt atcttttttc ttgcagctag
     8581 taaaagcaat taaagaacct aaagctgcta ctaaaattat tgataatata tttttcataa
     8641 ttataaattt tttaaattaa tactaaatta ttttttttaa ttaaaaatgg aacataataa
     8701 atagtgagcc acttgtgaaa gtgtcaattc ctacaaaact atcaatagca atttcttcag
     8761 tttttacttc acgtcctgtt cctgtccatg tttcagtagt tacaaaacca ccttctttag
     8821 aaatgttgaa tcctaatccc atttctccac caatagaaat tttaggagcg aagaaatatt
     8881 caacaccaac aacgccacgt aaagaaaaac ctaaattgtc attgttatgt acagaaagaa
     8941 tacgttggct tcctgcaact tctgcaccag cagtaaagtc ataatatgaa ggagaagtga
     9001 attctgcagt catttcgtta ccatacttgt aattaacatt tcttgagttg tagttaaaga
     9061 aagcacctcc accatagtat ccttgtactc tacctttacc acgatacatt aagtaatcac
     9121 caccaagaac aatgtttgta gcgttaacgt gcattacgtc agtaacttta gcgtctggat
     9181 ttggaattgt catgttcttg gtaacaaatt ctctgtcata gagattagtg tgtccaagtc
     9241 ttacagatcc tctaatagct acttggtcgt ctaagtaata cttaccgtag attgttaagg
     9301 cagttctgtt aatagttggg ataaagttga aagccccact attacccata gttccgttaa
     9361 aaaggtttcc aaaataatca actacaggca tagcatcaat accaagagca aattcgcctg
     9421 cttttggtaa aatgttttcg ccacgttttg atttcatgtc aacctcttgt gcgaataatc
     9481 ctgaaacaag gaatattgca caaataaaaa ttgttaagtg tttcataaat caaattaagt
     9541 ttagttaaac attaaaattg tcagtacaaa gatactaact tttttgtaaa aaatacgatt
     9601 tgtggataaa tttaatgttt ttttgagaat ttgatgccta tgtgttttaa atcaatggtt
     9661 tatatgttgg gaaggtttaa aattttgttg tttataaaaa aaactgcttt attaaaatag
     9721 gtaataaagc agtttttttt atatgtttta ataaaattct atcgtttatg acgaggttcg
     9781 tcagaaactg ttaatttttt tctgcctttt gctcttctag cagctaatat tttacgacca
     9841 ccaactgttg ccattctttc tctgaaaccg tgtttgtttt ttctttttct gttagaaggt
     9901 tgaaatgtcc ttttcatttt tatatctttt taaaacgtta tcaataaatt tttatggact
     9961 gcaaaaatag tatttttatt ttttattcaa aaatttttgt tgtttttttt gactaattgg
    10021 taaataaatt attttttttg tttcaaagta ttcataatca aacagttcgg agatgttgta
    10081 aatttgaaaa tttttaaaat tttttagttc ttcatcaaaa ttaccaccct ttaaataaat
    10141 aattccttgt ttattatgtt ctgttttgct taaaattatg ttttttgcga tattataaaa
    10201 ctttgggaaa gcggttacag ctctgctcac aacaaaatcg tattttggca ttaatgtttc
    10261 agctcttgca ttgatggctg ttatattttg caaatcaagt tgttctttta tagcggatac
    10321 aacacttatt ttttttgcga tgctgtcaat taaatcaaat tttgtttggg gaaacattat
    10381 tgcaagtggt attcctggaa atccaccacc agtacctatg tctgcaatag agtgagtttt
    10441 atcaaaatta aactttacag ctatcgagag agaatgtaaa atatggttga caaataagtt
    10501 gtctatgtct tttctgctaa tacaattaat tttattattc cattcaggaa aaattatttc
    10561 taattgttca aattgtttaa gttgtgtttg gttaatttgc ggaaaatatt ttaatagaac
    10621 ttcttgcatt ggtttatttg tatttgtttg tcaagagtgg ggctaataat tctctagctc
    10681 tatataattg tgcttttact gtacctattg gtaaatttaa ttcttcagca atttcttcat
    10741 aagtgaattc ttggtaatat cttagttcta ttagtgttgc atatcttggc tttaaagttt
    10801 ttattacact ttttaagtct tttgattttt gttttttaat taataagtct tcgggattag
    10861 gatttggaga tgtgaaattt gggttatcag aattgtgata ttcagatgtg ctgtcaattg
    10921 attgaggtct aacttttact tttctaacaa aatcaatgca attatttatc gcaattctaa
    10981 ataaccatgt actaaaagca tagtcggggg tgtatttatc aagattgtta aatgcttttg
    11041 caaaagtttc catagttaaa tcttctgcat cattttcgtt tccaattttt tttaaaagta
    11101 aaaaataaat tgggtcttga taacggtcta ataattgagc aaaggctttt tcattacctg
    11161 ataaagcttg ttcaacaatt tctaaatctt tgctggcttt tgctgaaaaa ttactgcgtt
    11221 ctaccattga tttttatctg ctttaaattt tttttgaatt aaaatttgaa aataaaaaat
    11281 tattgcaaag ttatcaaaaa taatgatata aaaaattagt agtttatttt ttatttttac
    11341 actaaatttt tttaaaataa cagagataat taatattctt aatatcaaaa ttatgcttgt
    11401 aattaaaaga aaagcattgt ttgagagtaa aattattgca gatattaaaa ataaaaatct
    11461 actaattaat tcagagccag aaattatttt ttcgtataaa ttatattttt tagaagtgct
    11521 taaatgtcgt gttttttgct taacaaacgc tttataagtt tccttagttt ttgataaact
    11581 tatactttca tgatgagtgc atattgctgt attttgcttt gtcgcaactt caataataaa
    11641 taaatcatca tcgccacttg caatgtctaa atgactttcg aaacctgaat tttctatcca
    11701 taatttttta gaataagcaa gatttcgccc aactcccata tatggtttta gctttttagc
    11761 tgatgacata tatgttagtg caattagttg agcatcatag cgaataaaag cgtttaataa
    11821 attgttttca agttcaaaca ttcctaaacc taaaacaatt tttttattag ccgaaaattt
    11881 tgagcaaata tgtcttagcc agttattatg tgcaggatag caatcagcgt caataaaaac
    11941 tagtttttcg tgttttgcat tttctattcc taagcttagt gcatgttttt tacctatatt
    12001 ttgagtttga gtttgaagaa tttttaaaat tttattttct tcttgaatta attctaacac
    12061 ttctaaagaa ttgtcagtac attggtctaa aacaacaata atttcaaatt tgttaaagtt
    12121 ttgatttaaa atttttggaa gatttttttc taagttttcg gcttcgtttt ttgctgcaat
    12181 aattatgctt actggctcgt cgttagttga agcagatagt ttttctttct taaaaattac
    12241 acttggaaat aaataaagat aataaaataa ttgaacactt gtagaaatgc aaaatactat
    12301 aaaaattatt atgagaaaaa tgctcattct atttatttaa tagctttact ctcttcccaa
    12361 atttcgttca tttcgtccaa agtcatgttt t
//
LOCUS       DAOPNX010000076        12190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_6040_length_12190_cov_5.535806, whole genome shotgun sequence.
ACCESSION   DAOPNX010000076 DAOPNX010000000
VERSION     DAOPNX010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12190
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6040_length_12190_cov_5.535806"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3419
                     /locus_tag="PKN53_08500"
     CDS             <1..3419
                     /locus_tag="PKN53_08500"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOD89027.1"
                     /translation="NSQDQSNLCAGNYSVAILDNNNCYKTESFVIDAPPFITISLAAT
                     PTECYGDCTGSVTATPSGGTAPYTYSWSNGQTTATALNLCSELHTVTITDNNGCTAQN
                     SIMVNQPTAMQFTNVTINDAECNGSSNGDVSVSVIGGTPPYSYVWDNGQTSSNPFGLA
                     AGPHCVTVFDSHNCSIDTCIMVNQPPMLNVTLNATNELCNGACNGTITAYPSGGQGPY
                     SYLWSNTEVTQTIHNLCAGVYNLTITDANNCQAYSSTSINSPQVLGIAVQEVIQPTCG
                     NNNGSIRVGATGGTPSYTYAWSPVSGSSNTLSNIGSGNYTVTITDSHACSISQTVSLS
                     DINAPIITDILITNVDCYGNNTGSAEVIYTSSTAVNTISWSNSQNTALATNLVEGNYS
                     VTVTDDNSCAATENIVITQPQPLSAMITNHADITCFGFTNGQATVMAIGGSTPYIYSW
                     SGGDNTETTHTLQAGLAVVTVTDANGCQATASVNINEPSQLSITGTATNTSCYAGSDG
                     VITVHASGGTGNYIYSWPQIPANTNTITGLPATTYTVIVYDQVDVSCFVSESFVVNEP
                     ARINASIGTENSYCGLDNGVAYIANITGGTGSYTYSWNPGNMHSDYITNLAAGSYMLT
                     ITDANGCTREYNVNVNATPNFQLDNVLFNGVTCYGYNDGFAQIFVSGGSNPYTYNWTP
                     NVTDQSSSNELYAGIYNVQITDNDGCQVYTQIPISTPEELIVFPDEDKEICIGQSVII
                     SASASGGNGNYSFVWDGLGSGSAFYVEPTTSTNYTVTAFDWKGCFSQPGVQHVEVKPP
                     LSLNVITPAAICVGQASTLIANATGGDGNYIYDWGNGQVTTSNELLISPTTDTEFTVT
                     VRDGCTTPPATATVTASVSDMPEMHLSRTPYIGCVPLNVAFDNNTENMTYNYYWEFDD
                     TESGVNNFSNLKNPTHTFEANGLYKVMAVVTTSMGCKDTAYVNVNVHQGPIADFNAFP
                     WSTGLFEPEIEFSDASVGAVTWEWNFGDGNVSKAQNPTHTYMEQGEFPVTLTVASLHS
                     CLDSITKNIVINEDHRIYFPTAINLRSPGNDEFYPIGVGIDEENYEMTIYNRWGEPIF
                     TTKDWYTHWKGRYNMDKGDYVPEGVYTYIVKLRDKYGKDRVYSGSFMVFK"
     gene            3548..5263
                     /gene="recJ"
                     /locus_tag="PKN53_08505"
     CDS             3548..5263
                     /gene="recJ"
                     /locus_tag="PKN53_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOD89028.1"
                     /translation="MKHRWLLKPLGDTETVEKLSQELNVETEIAQLLVQRGINTYEEA
                     RVFFRPKLSDLHDPFLMKDMDKAVERLSYAIKNNEKILIYGDYDVDGTTAVATIYNYL
                     IRNYQNIEYYIPDRYLEGYGLSFKGIDYAEEINCKLIITVDCGIKAIEKTNYANQKNI
                     DLIICDHHTPGPELPNAYAILDPKREDCNYPYKELSGCGVAFKLLQAFCQKTNDSFEE
                     LAKLLDLVVVSIGSDIVPITGENRTLAYFGLSQLNTNPQLGLKSIINIAGLENKEIQI
                     NDIVFKIGPRINAAGRLESGKTAVELLTSTNEAYVNKIAVEIDCINTNRKELDAEITE
                     HALSMINESEISKNAFSTIVYHEEWHKGVIGIVASRLTETYYRPTVVFTKSNDIITGS
                     ARSVPGYNLYEAIEACGDLLENFGGHRYAAGLSLKPENFNAFKERFEKYVMETITPEQ
                     RIPVINADLEIKIKDITPKFFRILKQFAPFGPENMTPTFITRNIFDNGSAKIVGKDEE
                     HLKLKIVQKLGDNRCIDAIGFNFANYYQHIKEFNEFDICYQIFENNFMNQKFIQILIK
                     DISTN"
     gene            5500..6297
                     /locus_tag="PKN53_08510"
     CDS             5500..6297
                     /locus_tag="PKN53_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOD89029.1"
                     /translation="MKKSILVPYDFTEVGNYALEHAYMVGKASRTPISIIHIVKKTSE
                     IEEAKAKLEKIAKDFSSQKDIEVVAEVRKGNLYKEIYKYGLEIDAYIAVMGTHGVKSV
                     KKALKVVKKFIKIPFILVQSPVIFGEYDRILIPIDQDNTARIKIQWIRYINTLFKSKV
                     YMITYNVSDSYGQKNISSNIRFAENLFEKELIDYEIEVLESSRNFADSIFTYANEVES
                     DLIMLMSDKYRSYVKQIKKAENLELYKKIPIMCVNKRLDIFKMGGFN"
     gene            complement(6294..6782)
                     /locus_tag="PKN53_08515"
     CDS             complement(6294..6782)
                     /locus_tag="PKN53_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89030.1"
                     /translation="MYISTSEQHIEFLKNTVLKGVENAFENNQLIFGFMSLAQAIEIL
                     GAYLDDKPLRAKKQSLKRFSLAINRLFPKEYSKANDKNFLYYQLRACMTHFFIPTSRL
                     SLNLGRDNKEKPHLSVIDGVMLIYHENLLTDFRFAVSILEKRILEGKLKLKPISFGKV
                     ND"
     gene            complement(6782..8860)
                     /locus_tag="PKN53_08520"
     CDS             complement(6782..8860)
                     /locus_tag="PKN53_08520"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOD89031.1"
                     /translation="MRFKVFFLIVFVLSSFLAHAQEETIETLDEIIEEIASNTDYELD
                     YTTLFQAFEEYYYNPIDLNSANESDLKDLIFLTEFQIFSLIKYRQKFGMYVSLYELQF
                     VDGIDDLTLKRLLPFVCIREAKPSEIKDFKRIFTYGKHVGIVRYQRLLQEKAGYKEIP
                     DSVLALNPNKSRYLGSPDKLYYRHNYHYKDKIFYGITAEKDDGEQFFKGAQKYGFDFY
                     SAHFQLNRVGAFQKIIVGDYIAQFGQGLTMWSGMSFGKTSSSTNVIKNSRGVHKYSSV
                     NESAFLRGQAATVQFGKFNFTEFVSYKSLDAGVDTIADIEFESEEHAISSFLETGYHR
                     TPSEVAKRKTIKEFIAGANMNWKEKNFKIGATGVYYGFSSPLGERTEPYRYFDFAGTK
                     NANFGVDYLVTIPKVNIFGETSMSSNLGFASVNGAVINFVPEFNMSVIHRYLRADYQA
                     LYAQPFSESSKPNNENGLFIGAELCPVKKWKIDAYFDSWKHPWLKYGIGAPSSGNEYL
                     VQVTHFPQRNLEIYLRTKYKTKQKNDPEIATGVRPIVDYSTLRTRLQLNYTPNPEWKF
                     KSRIEVSRYYMSSPEWGYLVYQDIDYKPKNIALKFNFRIAVFETETYNARLYAYEPDV
                     LYGFSVPAYYGRGSRLVFVVKYTLNQHFDFWFRITNTYYADKQSLGTGLDAIDGNNRT
                     DVKLQLRYKF"
     gene            complement(8850..9680)
                     /locus_tag="PKN53_08525"
     CDS             complement(8850..9680)
                     /locus_tag="PKN53_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89032.1"
                     /translation="MKKIFFLILLIVISACAFSQSNSYSAGARAAGMANSGLTNIDAW
                     AIFNNPAAHSFLKQREVGLYYENKFLLKQTGYGAMAFTTPLFGGSLGVGFSHFGYSLF
                     QNDKFTLGYSQQLFKSFSLGVQLNYFSVRQAEDYGNLNAMSFEIGILSKPNDKLSIGA
                     YAFNPVNLGYFEDSDLKMPITLRLGISYLFSKSLLLSLETGKSINGYTPVFKTGLEYL
                     INEQFAMRAGLALKPIEYSFGMGYNTNLGSSSKIGFDLAFAYHQVLGSSPKISISYAF
                     "
     gene            complement(9689..10195)
                     /gene="purE"
                     /locus_tag="PKN53_08530"
     CDS             complement(9689..10195)
                     /gene="purE"
                     /locus_tag="PKN53_08530"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOD89033.1"
                     /translation="MKAIVSIIMGSTSDWKIMEKAAQQLNDFKIPFEINALSAHRTPA
                     EVEEFAKNAESRGIKVIIAGAGMAAHLCGVIASMTKLPVIGVPINATLDGLDALLAIV
                     QMPPGIPVATVAINGAQNAAILAAQILALSDEELAKRVFEFKGNLKTKIVQANKDLQE
                     LKFEYRVN"
     gene            10314..10856
                     /gene="hpt"
                     /locus_tag="PKN53_08535"
     CDS             10314..10856
                     /gene="hpt"
                     /locus_tag="PKN53_08535"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOD89034.1"
                     /translation="MNKNKVKVFDKEFEIFISQTEIESKIVEIANQMNKDLKGEKPIF
                     LGILNGSFMFASELFKSLDIECSISFLKLTSYQGTSSTGKIKQLIGLSENLTGQTVVI
                     LEDIIDTGITLENIMKQITGFEPKKILIASLLYKSEAYKKTYPIDYYCFDIPNDFIVG
                     YGLDYNGHGRNLRHIYKIVE"
     gene            10868..12040
                     /locus_tag="PKN53_08540"
     CDS             10868..12040
                     /locus_tag="PKN53_08540"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF011105.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOD89035.1"
                     /translation="MLNIALFGPPGAGKGTQAKMLAKKYNLTYISTGDILRNEIANGT
                     ELGLSAKSTIEQGGLVCDETIVQIIENSIKMQPDSNGILFDGFPRTYTQAYILDGLLT
                     KMNFKLSCLISLEVPRDELINRMRLRAKQENRPDDKKEIIENRLREYDIKTIPVIDFY
                     KQQNKFHAINGIGNVDEVFERLTNKIEEVLSQTLLNVIFLGKPGAGKGTQARRIAKRF
                     NMKYVSTGEMIRREIENQTELGKIAQPYLATGDFVPDDIAIRLLEREIQNPFKGTGYL
                     FKGFPSTLVQAYILGGLLQKQETSVSLVVEIQTSTLQAIKRLRRRANTDMARIYDMDQ
                     DTILYRLENHEEKGHKVVDFYKQQNKIVSFNGNQDEDDLFEEISETIEKAFKEIRK"
BASE COUNT         4471 a         1969 c         1884 g         3866 t
ORIGIN      
        1 ctaactctca agatcaatct aacttgtgtg caggaaatta ttcagttgct attttagata
       61 acaataactg ctacaaaaca gaaagttttg taattgatgc tccaccattt attactattt
      121 ctcttgctgc aacaccaaca gaatgctatg gagattgtac tggttcagta acagcaactc
      181 cttcgggtgg tactgctcca tatacttact catggagtaa tggacaaaca acagcaacag
      241 cattaaattt atgttcagaa ctacatactg ttacaattac cgacaataat ggctgtactg
      301 cacaaaactc aataatggtt aaccaaccaa cagcaatgca attcacaaac gtaacaataa
      361 acgatgcaga atgtaatggc tcttcaaatg gtgatgttag cgtatcagtt attggaggaa
      421 ccccacctta ttcttatgtt tgggataatg gacaaactag ttccaatcct tttggactag
      481 ctgcaggtcc acattgtgtt acagtatttg acagtcataa ctgttctatt gatacttgca
      541 ttatggttaa tcaaccacca atgttaaatg taactcttaa tgctacaaat gaactttgca
      601 atggagcttg taatggtacg ataactgcat atccatctgg aggacaagga ccatatagtt
      661 atttatggtc aaatacagaa gtaacacaaa caatacataa cttatgtgct ggtgtttata
      721 atttaactat tacagatgcc aataactgtc aagcatatag ctcaacaagc attaatagtc
      781 cacaagtatt aggtattgct gttcaagagg taattcaacc aacttgcgga aacaataatg
      841 gctctattag agttggtgca actggtggaa caccttcata tacttatgct tggtctcctg
      901 tttctggaag ttcaaatact ttgagtaata taggctctgg aaattatacc gtaacaatta
      961 cagacagtca tgcttgtagt ataagtcaaa cagtatcatt aagcgacatt aatgcaccaa
     1021 taattactga tattttaatt acaaatgttg attgttatgg caataatact ggaagtgcag
     1081 aagttattta caccagttca acagctgtta acactatatc atggagcaat tctcaaaata
     1141 cagctcttgc tacaaaccta gtagaaggga actactctgt tacagttact gacgataata
     1201 gttgtgcagc aacagaaaac atagtaatca cacaaccaca accacttagt gcaatgatta
     1261 ccaaccacgc tgatattact tgctttggct tcacaaatgg acaagcaact gtaatggcta
     1321 ttggaggtag tacaccatac atctactctt ggagcggtgg tgataatact gaaactacac
     1381 acacattaca agcaggctta gctgtagtaa cagtaactga tgcaaatggt tgtcaggcta
     1441 ctgcaagtgt aaatataaac gaaccaagcc aattaagtat tacaggtact gctacaaaca
     1501 cttcttgcta tgcaggtagc gacggcgtaa ttactgtaca tgctagtggt ggaacaggaa
     1561 actatattta ctcttggcct caaattccag ctaacacaaa cactatcact ggactgccag
     1621 ctacaacata tactgtaatt gtttatgatc aagttgatgt tagctgtttt gtctctgaaa
     1681 gttttgttgt aaacgaacct gccagaataa atgcttcaat aggcacagaa aactcttatt
     1741 gcggattaga caatggtgta gcttatatag ctaacattac tggtggaaca ggaagctata
     1801 catattcttg gaatccaggt aatatgcaca gcgactacat aactaaccta gctgctggta
     1861 gctatatgct gacaattact gatgctaatg gctgtacaag agaatataat gtaaatgtaa
     1921 atgcaacacc taatttccaa ctagacaatg ttttatttaa cggtgtaaca tgttatggat
     1981 ataatgacgg ttttgcacag atatttgtat ctggaggttc taatccttat acttataatt
     2041 ggactccaaa tgtaacagac caatctagtt caaacgaatt atatgcaggt atttacaatg
     2101 tgcaaattac agataatgat ggatgtcaag tttatactca aattccaatc agcacaccag
     2161 aagaattaat agtcttccct gacgaagaca aagaaatttg tattggtcaa tctgttatta
     2221 tatctgcttc tgcaagtggt ggaaatggaa attactcttt cgtttgggac ggattaggct
     2281 caggctctgc attttatgtt gaacctacaa ctagtacaaa ttatactgta acagcatttg
     2341 attggaaagg gtgcttttct caacctggag tacaacatgt agaagttaag cctcctctat
     2401 cacttaatgt tattactcct gctgcaattt gtgtaggaca agcatcaaca ttaattgcaa
     2461 atgctacagg tggagatggc aattatattt acgactgggg taatggacaa gttactacat
     2521 ctaacgaatt actaataagc ccaaccactg atactgaatt tactgtaaca gttagagatg
     2581 gttgtacaac acctcctgct actgcaacag taacagctag tgtttctgat atgccagaaa
     2641 tgcatttaag tagaactccg tatataggtt gtgttccttt aaatgttgct ttcgataata
     2701 atactgaaaa tatgacttac aattattatt gggagtttga tgacacagaa tcaggagtaa
     2761 ataacttctc taacctaaaa aatccaactc atacttttga agcaaatgga ttgtacaaag
     2821 taatggcggt agttacaaca tctatgggct gtaaagacac tgcttatgtc aatgtaaatg
     2881 ttcatcaagg acctatcgct gatttcaatg cgttcccatg gtcaacagga ttatttgaac
     2941 ctgaaattga attttctgac gcatcggttg gtgcagttac ttgggaatgg aactttggtg
     3001 atggaaatgt atcaaaagca caaaatccaa cacatactta tatggaacaa ggagagttcc
     3061 ctgttacatt aactgtagca agtcttcata gctgtttaga ctctattaca aaaaatattg
     3121 ttataaatga agaccataga atttatttcc caactgcaat taatttaaga agtcctggta
     3181 atgatgaatt ctatcctatt ggagttggta tagatgaaga aaactatgaa atgacaatct
     3241 ataaccgatg gggagaacct attttcacaa ctaaagattg gtacacccat tggaaaggac
     3301 gctataatat ggacaaaggc gattatgtac cagaaggtgt atatacatat attgtaaaac
     3361 taagagacaa atacggtaaa gacagagttt attctggctc atttatggtt tttaaataat
     3421 tactgatgtt aaatttcagt aaaattttaa aacttttaac atacactgtt gattaaaaaa
     3481 atataaaatc ttagaataag acatttattt tgattattat tgtactcaat aaaatcaata
     3541 taagatgatg aagcaccgct ggctattaaa acctttagga gacaccgaaa ctgtagaaaa
     3601 attatcacaa gaattaaacg ttgaaacaga aatagctcaa ctacttgttc aaagaggtat
     3661 taatacttat gaagaagcaa gagtcttttt tagaccaaaa ctaagcgacc tacacgatcc
     3721 ttttttaatg aaagatatgg ataaagcggt tgaaaggcta tcatatgcaa ttaaaaataa
     3781 cgaaaaaata cttatttatg gtgattacga tgttgatgga actactgctg tagcaacaat
     3841 ttacaattat cttattagaa actatcaaaa tattgaatat tatattccag accgctatct
     3901 agaaggatat ggactttcgt ttaaaggaat tgattatgct gaagaaatta attgcaaatt
     3961 aataattact gttgactgtg gcataaaagc aattgaaaaa acaaattatg caaaccaaaa
     4021 aaatatagat ttaataattt gcgaccatca cacacctgga cctgagctac caaatgccta
     4081 tgctatttta gacccaaaac gagaagattg taattaccct tacaaagaac tatctggttg
     4141 tggtgtagct tttaaattat tgcaggcatt ttgccaaaaa acaaacgata gcttcgaaga
     4201 gcttgccaaa ctcctcgacc ttgttgttgt tagtataggc tctgacatcg ttccaattac
     4261 aggcgaaaac agaacacttg catattttgg actatctcaa ctaaatacta acccacaact
     4321 tggactaaaa agcattataa atatagccgg attagaaaac aaagaaatac aaataaatga
     4381 catagtcttt aaaataggtc ctagaataaa tgctgctgga agattagaat ccggaaaaac
     4441 agctgttgaa ctattaacat cgactaatga ggcttatgta aacaaaattg ctgttgaaat
     4501 tgattgcata aatacaaata gaaaagaact agatgcagag ataacagaac atgcattaag
     4561 catgattaac gaatctgaaa tatcaaaaaa tgctttctca accattgttt atcatgaaga
     4621 atggcataaa ggagttattg gaattgtagc atcacgttta acagaaactt attacagacc
     4681 aacagtagta tttacaaaat ctaatgacat aattactggc tctgctcgtt cagtaccagg
     4741 atacaaccta tacgaagcta ttgaagcatg tggcgattta ctagaaaatt ttggcggaca
     4801 cagatacgca gctggactaa gccttaaacc agaaaacttt aatgccttca aagaaagatt
     4861 tgaaaaatat gttatggaaa caataacccc agaacaaaga attcctgtaa ttaatgccga
     4921 tttagaaatt aaaattaaag atattacacc aaaatttttt agaatattaa aacaatttgc
     4981 accattcgga ccagaaaata tgacacctac ttttataact agaaatatat ttgacaatgg
     5041 tagtgccaaa attgtaggaa aagatgaaga acatttaaaa ttaaaaatag tacaaaaact
     5101 tggcgacaat agatgtatag atgcaatagg ctttaatttt gccaattatt accaacatat
     5161 aaaagaattt aatgaatttg atatatgtta tcaaatcttt gaaaataatt ttatgaacca
     5221 aaagtttatt caaattttaa ttaaagacat atctacaaat taagatattg ctaaatacat
     5281 gaatatatac atgatgtatt ttaatctata ctcaacaaag atattaacaa tattatctta
     5341 atatttattc aaaaaaatat cttttatttg aagtaaattt atttattttg aattaaaatt
     5401 aaaattttag tgattataag caaccaaatt gtatataatt acgtattata attaagataa
     5461 gagatagttt aattattatg aaaatttaaa tatactaata tgaaaaaaag tattcttgtt
     5521 ccttatgact tcacagaagt tggcaattat gcactagagc atgcatatat ggttggaaaa
     5581 gcttcgagaa ctcccattag cattattcac attgtaaaga aaacttcaga aattgaagaa
     5641 gcaaaggcta aacttgaaaa aatagcaaaa gacttctcaa gccaaaaaga tatagaagtt
     5701 gtagcagaag ttagaaaagg aaatttatat aaagaaatat ataagtatgg attagaaatt
     5761 gacgcttata ttgctgttat gggaactcat ggagtaaaaa gcgtaaaaaa agcacttaaa
     5821 gtcgttaaaa aattcattaa aattcctttc attttagtac aaagtcctgt tatttttgga
     5881 gaatacgata gaatacttat tccgattgac caagacaata ctgctagaat caaaatacaa
     5941 tggataaggt atataaatac tttatttaaa tcaaaagtat atatgattac ttacaatgtt
     6001 agtgattctt atgggcaaaa aaatatatca agcaatatta gatttgctga aaacctattt
     6061 gaaaaagaac ttattgatta cgaaatagaa gttctagaaa gttctagaaa ttttgctgat
     6121 agtattttta cttacgcaaa cgaagttgaa agcgacttaa taatgttaat gtcagacaaa
     6181 tatcgttcgt atgttaaaca aattaaaaaa gctgaaaact tagaacttta caaaaaaata
     6241 cctattatgt gcgtaaataa acgcttggat attttcaaaa tggggggttt caactaatca
     6301 ttcacttttc caaaagaaat aggttttagt tttagttttc cttctagaat acgtttttca
     6361 agaattgata ctgcaaacct aaagtctgtt aacaaatttt catgataaat taacataacc
     6421 ccgtctatta cagatagatg gggtttttcc ttattatctc ttcctaaatt taaagaaaga
     6481 cgagaagttg gaataaaaaa atgtgtcatg caagccctta actgataata aagaaaattt
     6541 ttatcgttag cttttgaata ttctttcggg aacaaacgat ttatagctaa agaaaaacgt
     6601 tttaaagatt gcttttttgc tcttaaaggc ttgtcatcta aataagctcc aagtatttct
     6661 atcgcttgtg ctaaagacat aaacccaaat ataagctgat tattttcaaa agcattttca
     6721 acgcccttta aaactgtatt tttaagaaat tcgatatgtt gctcagaagt tgaaatatac
     6781 attaaaactt gtatcttaat tgtaatttta catctgttct attattgcca tcaatagcat
     6841 ctaagcctgt tccaagactt tgtttatcag cataataagt attagttatc ctaaaccaaa
     6901 aatcaaaatg ttgatttaat gtatatttca caacaaatac caacctactt cccctaccat
     6961 aatacgctgg cactgaaaat ccataaagca catcaggttc ataagcataa agtctcgcat
     7021 tatatgtttc agtttcaaat actgctatgc gaaaattaaa ttttaaagca atatttttag
     7081 gcttgtaatc aatatcttga taaactaaat atccccactc tggcgaactc atgtaatatc
     7141 tcgaaacttc aattctggat ttgaatttcc attctggatt cggagtataa ttaagctgta
     7201 atctagttct aagagttgaa taatcaacta ttggtctaac tccagtagca atttctgggt
     7261 catttttttg cttagtttta tattttgttc ttaaatatat ttctaaattt ctttgaggaa
     7321 aatgagtaac ttgtactaaa tattcatttc ctgaagaagg tgctccaatt ccatatttca
     7381 accaaggatg tttccaagaa tcaaaataag catctatttt ccattttttt acggggcata
     7441 attctgcacc tataaacaaa ccattttcat tatttggttt actagattct gaaaatggct
     7501 gagcatacaa agcttgatag tcggctcgta aatatctatg aataactgac atattaaact
     7561 ctggaacaaa atttataaca gcaccattca cacttgcaaa tcctaaatta gaactcatag
     7621 atgtttcgcc aaaaatatta accttaggaa tagttactaa atagtcaaca ccaaaatttg
     7681 cattttttgt tcctgcaaag tcaaaatatc tataaggctc ggtgcgttct cccaaaggac
     7741 ttgaaaaacc ataatatact cccgttgctc ctattttaaa gtttttttct ttccaattca
     7801 tatttgctcc agcaataaac tctttaatag tcttacgctt agcaacttcg cttggtgtgc
     7861 gatgataacc agtttctaag aaagaagaaa ttgcatgctc ttcactttca aactctatgt
     7921 cggcaatagt atcaactcct gcatctaaac ttttatatga aacaaattca gtaaaattaa
     7981 attttccaaa ctgcactgtt gctgcttgtc cacgtaaaaa tgcggactca tttacagaac
     8041 tatatttatg cactcctctc gaatttttaa taacatttgt tgaagaagaa gtttttccaa
     8101 aactcattcc cgaccacatt gttaaacctt gaccaaactg agcaatataa tctccaacaa
     8161 ttattttttg aaaagctcct actctattca gctgaaaatg agcagaataa aaatcaaatc
     8221 catatttttg tgctccttta aaaaattgtt caccgtcatc tttttcggca gtaattccat
     8281 aaaatatctt atctttataa tgataattat gcctgtaata cagtttatct ggacttccta
     8341 aatagcgaga cttatttgga tttagtgcca aaacgctatc aggaatttct ttatatccag
     8401 ccttttcttg caaaagtctt tgataacgaa caattccgac atgttttcca taagtaaaaa
     8461 ttcgtttaaa atcttttatt tcagaaggtt tagcttctcg tatgcaaaca aaaggcaaaa
     8521 gtcgttttaa tgttaaatca tcaattccat ctacaaattg taattcatac aaacttacat
     8581 acatgccgaa tttttgacga tattttatta aagaaaaaat ttgaaattca gttaaaaata
     8641 ttaaatcttt taaatcgctt tcgttggcag aattcaaatc tattgggtta taataatatt
     8701 cttcgaaagc ttgaaacaat gttgtatagt caagttcata gtcggtgtta gacgcaattt
     8761 cttcaattat ttcatctaga gtttctatcg tttcttcttg ggcatgtgca agaaaagacg
     8821 acaaaacaaa aacaattaga aaaaatactt taaaacgcat aactaataga tatttttggg
     8881 cttgaaccta gaacttgatg ataagcaaat gctaagtcaa agccaatttt actactactt
     8941 cctaaatttg tattatatcc cattccgaaa gaatattcta ttggttttaa agctaaacct
     9001 gcacgcattg caaactgctc atttatcaaa tattctaagc ctgttttaaa cactggagta
     9061 tatccattta ttgactttcc cgtttccaaa gaaagtaaca aagatttact aaataaatat
     9121 gaaattccta atcttaaagt aatgggcatt tttaagtctg aatcttcaaa atatcccaaa
     9181 tttacaggat taaacgcata agcacctatt gaaagtttat cgttaggttt tgataaaatt
     9241 ccaatttcaa agctcattgc attcaaattt ccataatctt cggcttgtcg cacagaaaaa
     9301 taatttaact gaacgcctaa agaaaaagat ttgaacaatt gttgagaata tcctaaagta
     9361 aatttatcgt tttgaaaaag tgaatatcca aaatgagaaa atcctacacc taaactacca
     9421 ccaaataatg gtgtagtaaa agccatagct ccatagcctg tttgttttaa aagaaattta
     9481 ttctcgtaat aaagtcctac ttctctttgt tttagaaagc tatgagcagc cggattgtta
     9541 aaaattgccc aagcatctat atttgttaat cctgaattag ccattccagc cgctcttgca
     9601 ccagccgaat aagaatttga ctgagaaaaa gcacatgccg aaataactat taataatatg
     9661 agaaaaaata tttttttcat tttaaaaatt aatttactct atattcaaat tttaattctt
     9721 gcaagtcttt atttgcttga acaattttag ttttcaaatt tcctttaaat tcaaacactc
     9781 tttttgcaag ttcttcatca ctaagagcaa gtatttgtgc tgccaaaata gctgcgtttt
     9841 gtgctccatt aatagctact gtagccacag gaatacctgg aggcatttgt acaattgcca
     9901 ataaggcatc taatccatct aaagttgcat ttattgggac accaatcaca ggtaacttag
     9961 tcatagaagc aattacgcca cataaatgag ctgccattcc agctcctgca attataactt
    10021 ttattccacg agattcagca ttttttgcaa actcttcaac ctctgcaggt gttctatgag
    10081 ccgataaagc atttatttca aaaggaattt taaaatcatt taattgctga gcagcttttt
    10141 ccattatttt ccagtcggaa gtagaaccca taattatact tacaatagct ttcatatctt
    10201 taaatttttt attttaaaaa taacatggta aaaatacaaa gtatttatta cttttgccat
    10261 atattttaaa attttcttaa aataaaatgc aatttgctaa ttaaatgtaa gttatgaaca
    10321 aaaacaaagt taaggtattt gacaaagaat tcgaaatttt tatttcgcaa acagaaatag
    10381 aaagtaaaat tgtagaaatc gcaaatcaaa tgaataaaga tttaaaaggt gaaaaaccaa
    10441 ttttcttagg aattttaaat ggttcgttta tgtttgcttc tgaacttttt aaatctttag
    10501 atattgaatg cagtatatct ttcttaaaat taacctctta tcaaggaacg tcatctacag
    10561 gcaaaatcaa gcagttgata ggtttaagcg aaaatttaac tggacaaact gttgttattt
    10621 tagaagatat aattgatact ggcatcactt tagaaaatat tatgaagcaa attacaggtt
    10681 ttgaacctaa aaaaattcta atagccagtt tattatataa atctgaagca tataagaaga
    10741 catatcctat tgattactac tgctttgaca ttccaaatga ctttattgtt ggctatggac
    10801 ttgattataa tggacatggc agaaatttaa gacatattta taaaattgta gaataaattt
    10861 aagaatcatg ttaaatattg ctttatttgg tcctcctggt gcgggaaaag gcacacaagc
    10921 caagatgttg gcaaaaaaat acaacttaac ttatatttca acaggtgata ttctaagaaa
    10981 tgaaattgca aacggaactg aattagggtt aagtgctaaa tctactattg aacaaggtgg
    11041 attggtttgc gacgaaacta ttgttcaaat tattgaaaat agcataaaaa tgcaacccga
    11101 ctctaatggg attttgttcg acggttttcc tcgtacctac actcaagctt acattttaga
    11161 tggtttatta acaaaaatga acttcaaact atcttgttta atcagtctcg aagtgccaag
    11221 agatgagcta attaacagaa tgagacttag agcaaaacaa gaaaatagac ctgacgacaa
    11281 aaaagaaatt atcgaaaatc gcttgagaga atatgatatc aaaaccattc ctgttattga
    11341 tttttataaa caacaaaata aatttcatgc tattaacggt attggcaatg ttgacgaagt
    11401 ttttgaaaga ctaaccaata aaatcgaaga agttttatcc caaactttac ttaatgttat
    11461 atttttagga aaaccaggtg ctggaaaagg tacgcaagca agaagaattg ccaaaagatt
    11521 taatatgaaa tatgtttcta caggcgaaat gattcgtaga gaaatagaaa accaaaccga
    11581 acttggcaaa attgcacaac catatttagc aactggagat ttcgttccag acgacattgc
    11641 tattagattg ttagaaagag aaattcaaaa tcctttcaaa ggaacaggtt atttgtttaa
    11701 aggttttccg tcaactttag tgcaagcata tattttaggt ggattgttgc aaaaacaaga
    11761 aacttctgta tctcttgttg tcgaaattca aacatctacc ctacaagcaa tcaaacgtct
    11821 cagaagaaga gcaaatactg atatggcgag aatttatgat atggaccaag acactatact
    11881 ttatagattg gaaaatcatg aagaaaaagg tcataaagtt gtagattttt acaaacaaca
    11941 aaataaaatc gtaagtttta acggaaatca agatgaagat gacttatttg aagaaatttc
    12001 tgaaactata gaaaaagcat ttaaagaaat tagaaaataa ggcttacaaa aacagactaa
    12061 ctactgccaa aataaggcaa attatgtata aaactataat taacaataat ttatgacgat
    12121 aaattcagat aacatgaaat cttatattca cgtatatact ggaaatggta aaggaaaaac
    12181 cactgccgct
//
LOCUS       DAOPNX010000077        12127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_6085_length_12127_cov_8.260355, whole genome shotgun sequence.
ACCESSION   DAOPNX010000077 DAOPNX010000000
VERSION     DAOPNX010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12127
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6085_length_12127_cov_8.260355"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..346
                     /locus_tag="PKN53_08545"
     tRNA            265..346
                     /locus_tag="PKN53_08545"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:299..301,aa:Leu,seq:tag)
     gene            425..1768
                     /gene="tig"
                     /locus_tag="PKN53_08550"
     CDS             425..1768
                     /gene="tig"
                     /locus_tag="PKN53_08550"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOD89036.1"
                     /translation="MEVVLNQIDELNATLTVNVADSDIEERVEKTLKDYRRKANVPGF
                     RPGNVPFGLIKKMYGQAVRVEELNKTVSNAINDYIVENKLNVIGDPLPNESQKQIDLE
                     NKEFSFVFDIALAPEFEVKLSKKDKLTKYVITVDDEMVNSEIERYKQRMGEVADIKKS
                     TEKSILKVDMIELTENGEVKENGLELVDSSISVNVIKDEKIKKELVGIEANTIKDIDI
                     KLAYPNETELSSLLGIAKEELENTSNLFRITVKLVREYKAGELNQKLFDAVYGENEVK
                     SEEEFVERVKEDLIDTHSRDTNYKLSLDAKDKVVKKTEVALPEAFLERWIKASNQELK
                     EEELKNEFPRFLEHLKWTLIKTNIAKANELKVNNEDLLEVAKERIRAQVAQYGIMSIP
                     EEFLEKHAKESLEDKELVNKLYEQALEEKVIDYIKEAVKVEDKEISLEEFYKLFT"
     gene            complement(1774..3768)
                     /gene="ligA"
                     /locus_tag="PKN53_08555"
     CDS             complement(1774..3768)
                     /gene="ligA"
                     /locus_tag="PKN53_08555"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOD89037.1"
                     /translation="MENIELRIKKLSEELNKHNHNYYVLNNPSISDFEFDKLLAELID
                     LENKYPQFKQADSPTQRVGSDISNVFKTVTHEFPMLSLGNTYSAEELNDFHNRIVKEI
                     EIDPEYVCELKFDGASISVSYENSKYLRAVTRGDGTQGDDVSENIKTIRSIPLSISGE
                     NIPQNFEIRGEIIMPHKVFDELNEIREENGESPFANPRNATSGSIKMIDSREVAKRNL
                     DCYFYYMMGKNLSATTHWESLQQLKSWGFKISEHSKLCKTISEVYNYIEFWDEERKNL
                     SYDIDGIVIKVNSFAQQEILGFTAKSPRWAIAYKFKAEQVSTKLLSIDYQVGRTGAVT
                     PVANLEPVQLAGTVVKRASLHNADQIALLDIRINDIVFVEKGGEIIPKIVGVESHDKN
                     SSPTQFITHCPECGTQLYRPEGEARHFCPNSDHCPPQIKGKIEHFISRKAMNIENIGE
                     ETVALLYDTGLVRSISDLYKLKKEDILPLDRMAEKSANNIINSIEKSKEVSFDRVLFA
                     LGIRFVGETVAKILAKAFKNILNLSIASKEELMQVDEIGDRIADSIIEYFNNKEHTQL
                     IADLMNAGLQFELQEDENESLSDKLQGMSIIISGTFKNYSRDEIKSVIEQHGGKNVSS
                     ISKNTDLFVAGENIGPAKLKKATELNIKIVSEEEFIKMIE"
     gene            3856..5061
                     /locus_tag="PKN53_08560"
     CDS             3856..5061
                     /locus_tag="PKN53_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89038.1"
                     /translation="MKRLFSIQFLLSILVLTVAITSCSENKKSKKLDEQQILEQNNIE
                     VAKKLVNDFFDLLNKENYETCYSHFSDSLKQYFSKENLITTIKARNTEFPNSDSLGIY
                     CTQVLTTEIGDVYFFYYRVFSKSKLIFYEKISLYFQEGLPQILGYDYSENIFYTPKMA
                     NDADSDLKLVLQNMYQILNSGDIENAYAMVDETVKEKLSFSKFEAGFNEKKSKYQGTN
                     SFSVKSIWTEILYDVPVLNLIVDVVNYSGDRFLDKIMISDRDGKFFIATIDREDFEKA
                     ELIKIERLPDAELNKYAEVASKFYEFLKNSNFYEIMQMLDRTVFINNDYETIKSSFSA
                     REQYYGKPKNLKNTLVKTYSLGSKTLVEFNFDVENTNGKISYEKIIVSKTTEDKYFIF
                     SYDFRDTRF"
     gene            complement(5080..5604)
                     /locus_tag="PKN53_08565"
     CDS             complement(5080..5604)
                     /locus_tag="PKN53_08565"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89039.1"
                     /translation="MKVFRIAVLFVYILAALGVASCKDKSKVEEVTSKQQDNPIIKHD
                     ALAQSSDVKIVESFENENPLKVHFVDKEDEKQAYEKQYYENGNLFIEGLLVDGKREGK
                     WTAYYQNGVVWSIGYYENGLKQGRSEVFYENGKVRYVKSYKDDLADGLWQFYDDKGNM
                     IGEEMYKEGKKVVE"
     gene            5775..7175
                     /locus_tag="PKN53_08570"
     CDS             5775..7175
                     /locus_tag="PKN53_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOD89040.1"
                     /translation="MVKQTTISKEVKIEGVSLHTGKPATLLIKPAEPNTGYVFVRTDI
                     ENNPKIQAVAENVVDTSRGTTIGKNGVNVSTIEHLLATTYALGIDNVIFEINGPEVPI
                     LDGSAKYFIEAYKNTEIIEQDAERIVFNIEENLSFVDEEKNIEISVYPDKTYKLQVLV
                     DYKSDAISNQYAVLNSLDEFKNEVSACRTFVFLSELEPLLKQDLIKGGSLDNAIVIVD
                     KECDQAEFDRLAKLFNRPSVEVQSSGVLNNIKLQFSNEPARHKLLDLIGDLALTGYRF
                     NANIIANRPGHYANVNIAKIIRQHLNEVKKNLAKANIPIYNPDEKPLMDVMQIQGFIP
                     HRFPFLLVDKILSLSETEVVGVKCITNDENFFQGHFPDEPVMPGVLQVEAMAQTGGIL
                     VLSTVPDPENYLTFFLKIDNVKFRRKVVPGDVLVMQLTLLEPIRRGIAHMLGKAYVGT
                     ELACEADMYAQIVKLS"
     gene            7185..7988
                     /gene="lpxA"
                     /locus_tag="PKN53_08575"
     CDS             7185..7988
                     /gene="lpxA"
                     /locus_tag="PKN53_08575"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOD89041.1"
                     /translation="MNQPLSFVHSAAKIANNVVVEPFVTIHKNVEIGEGTWIGSNVTI
                     MEGSRIGKNCRIFPGAVIGAIPQDLKFRGEETTAEIGDGTTIREFVTVNRGTAAKGRT
                     IVGSNCLIMAYVHVAHDCLIGDNVILVNGVNLGGEVIVDDYAIISANSLIHQFCRIGA
                     HTILAGNSLVGKDVPPYVKAARYPLSFAGVNSIGLRRRNFCNDKINAIQDIYRVIYNR
                     GMNVSQATAYIEAEMPASKERDEILLFIRNSKRGIIKGYFDKSESDVAF"
     gene            complement(8010..8759)
                     /locus_tag="PKN53_08580"
     CDS             complement(8010..8759)
                     /locus_tag="PKN53_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOD89042.1"
                     /translation="MREFGLIGYPLKHSYSHIYFENKFKNEKIDDAKYSLFPITEISK
                     LHNIISTKENLLGLNVTTPYKEQILPYLNDIDKSVLNIGTVNTIKIERNAEEIKLTGY
                     NTDIDGLRKTFEKIELPPINKALILGSGGSGKTVKFVLEELGIKSTTVSRNPKNLSVL
                     SYKNVTEAVIKHHNLIINASPVGMFPNVDNCPNIPYEYIKDTHICFDLVYNPEQTLFL
                     KNCENKGAKCINGLTMLYEQADRAWEIWNRD"
     gene            complement(8756..9337)
                     /locus_tag="PKN53_08585"
     CDS             complement(8756..9337)
                     /locus_tag="PKN53_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOD89043.1"
                     /translation="MNYTTITLLMTIESSFIPFPSEIVIPPAAYIASDPESDLNIFLV
                     VLFGTIGALIGAYINYFLALWLGRPIVYKFADSRLGRFFLLSKEKVVKAETYFNNHGK
                     TSTFIGRLIPGIRQLISIPAGLAKMNFLAFSIYTFIGAMIWNIILAALGYLAHGQKDL
                     IDKYNKELSIAIVVIVVLVILFFIIRKLIKRKK"
     gene            10552..10893
                     /gene="rbfA"
                     /locus_tag="PKN53_08590"
     CDS             10552..10893
                     /gene="rbfA"
                     /locus_tag="PKN53_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOD89044.1"
                     /translation="MEQSKRQLQVGNLVQKELSHIIQHKFNGLVPGRLLTVTNVKMTP
                     DLSIARISLSIFPSNDGEAIIKEINKQVSPIRFELGNALRNNLKKIPELVFYLDDSLD
                     QSEKIDQLLKK"
     gene            10897..12123
                     /locus_tag="PKN53_08595"
     CDS             10897..12123
                     /locus_tag="PKN53_08595"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOD89045.1"
                     /translation="MKFSFYIARRYLISKKSRNAINIISFISVLGVSVGTAALITILS
                     VFNGFDNLLTRLYNSFDPDIKIVAAKGKVFKFDDKVSEKLNLDFIEEYSLCLEENAMF
                     AYDDKQIVATIKGVDDNYKNVTGIDSMIVRGDYFLEYQNAFCGVSGQGIAYNLNLDVS
                     FIYPVKVYIPSRTKKISAGMIDAADNLNQSIVYNAGIFSIQQEYDTKYFIAPLSEVRR
                     LLEYENEVSSIEIKIKKGENIKTSLNKLTESFGEDFIVQDRNMQHEYAFKIMQSEKWA
                     IFMILIFILLIASFNIIGSLTMLIIEKKNDIKILKSLGAEDKTIRKIFFLQGLFISFI
                     GAIVGMLFGSVLCWAQIKFGLIKLGTGGSFIIDSYPVAMQAMDFVWVFIAVMIIGLIA
                     AWYPVRYITKKFSEVS"
BASE COUNT         4327 a         1815 c         1834 g         4151 t
ORIGIN      
        1 ttatttatac gttaaatttt taattttgtt gtcctaaaaa aggggaccat tatagtaaca
       61 acactccatt aaatttaagc caacttgttt caggaacata tctaatacaa atacaaacaa
      121 aacaaaatat atttagcaaa cgtatagtta aaaattagta aatcgatttg actttctatc
      181 aaattttttt ctccaaacaa ataaaaaaca aaaaaatgct ttttaattaa aaaaatgtat
      241 atatctttgc aactcaattt tgtggcggat gtggcgaaat tggtagacgc gctagactta
      301 ggatctagtg ccgcgaggcg tgggggttcg agtcccttca tccgcacaac aaccgcatga
      361 gtttcggaca agaaataaat gcggtttttt taacttttta taaattataa ctaaaacaaa
      421 acaaatggaa gttgtactaa atcaaatcga tgaactaaac gcaactctaa ctgtaaacgt
      481 tgctgattct gatatagaag aacgcgtaga aaaaaccctt aaagattatc gccgtaaagc
      541 aaatgtccca ggatttagac ctggaaatgt gccttttggc ttaattaaaa aaatgtacgg
      601 acaagcagtt cgtgttgaag aactaaacaa aactgtttca aatgcaataa acgactatat
      661 cgtagaaaat aaactaaatg ttattggcga ccctctacca aacgaaagcc aaaaacaaat
      721 tgatttagaa aataaagaat ttagttttgt tttcgatatt gctttagctc cagaatttga
      781 agtaaaactt agcaaaaaag ataaacttac taaatatgta attaccgttg atgacgaaat
      841 ggtaaattca gaaattgaac gctacaaaca aagaatgggc gaagttgctg atattaaaaa
      901 atcaacagaa aaatctatac taaaagttga tatgattgag cttaccgaaa atggcgaagt
      961 taaagaaaat ggtttagaat tagttgattc ttcaatttct gtaaacgtta taaaagacga
     1021 aaaaattaaa aaagaactcg ttggcattga agctaacact attaaagata tagatattaa
     1081 acttgcttat cctaacgaaa ctgaactatc gtctttatta ggtattgcca aagaagaatt
     1141 agaaaacaca agcaatttat ttagaattac agtaaaactt gtaagagaat ataaagctgg
     1201 agaattaaat caaaaacttt ttgatgctgt ttacggcgaa aacgaagtaa aaagcgaaga
     1261 agaatttgtt gaaagagtaa aagaagactt gatagatact cactctcgcg ataccaatta
     1321 taaattgtct ttagatgcaa aagataaagt tgttaaaaaa acagaagtag ctttaccaga
     1381 agcattctta gaacgttgga ttaaagcttc aaatcaagaa cttaaagaag aagaacttaa
     1441 aaacgaattc ccaagatttc ttgaacactt aaaatggact ttaattaaaa ccaatattgc
     1501 aaaagcaaac gaattaaaag ttaataacga agaccttctt gaagttgcta aagaaagaat
     1561 tagagcgcaa gttgctcaat atggaattat gagtattcca gaagaattct tagaaaaaca
     1621 tgctaaagaa agcttagaag ataaagaatt agtaaataaa ctttacgagc aagctttaga
     1681 agaaaaagtg attgattata taaaagaagc cgtaaaagtc gaagataaag aaataagcct
     1741 tgaagaattt tataaattat tcacctaatt tttttattca atcatcttta taaattcctc
     1801 ttcgctaaca attttaatat ttagttctgt agctttttta agttttgcag gacctatatt
     1861 ttcgccagca acaaataaat ctgtgttttt agaaatagag ctcacatttt taccaccgtg
     1921 ttgttcaatt acagatttta tttcgtcacg agaataattt ttaaaagtac cagaaataat
     1981 tattgacatt ccctgcaatt tatcgctaag cgattcattt tcatcttctt gtaactcgaa
     2041 ttgcaaacca gcattcatta aatctgcaat aagttgtgta tgttctttat tgttaaaata
     2101 ttctattatg ctatccgcaa ttctgtcgcc aatttcgtca acttgcatca gttcttcttt
     2161 gcttgcaata gagagattta aaatattttt aaatgctttt gccaaaattt tagccacagt
     2221 ttctccaaca aatctaattc caagagcaaa aagcactcta tcaaaactta cttcttttga
     2281 tttttcaata ctgttaataa tattattagc agatttttct gccattctat ccaaaggtaa
     2341 aatatcttct tttttgagct tgtacaaatc cgaaattgaa cgaactaagc ctgtatcata
     2401 aagtaaagca actgtttctt cgccaatatt ttcgatattc atagcttttc gcgaaataaa
     2461 atgctctatt ttccctttta tttgtggtgg acaatggtca gaatttggac aaaaatgtct
     2521 tgcttcgccc tctggtctat aaagttgagt tccacattct ggacagtgag ttataaattg
     2581 cgtaggcgat gaatttttat catgagattc tactccaaca attttaggaa ttatttctcc
     2641 acctttttct acaaacacaa tgtcgttaat gcgaatatct aaaagtgcaa tttggtcggc
     2701 attgtgtagc gaagcccgct ttacaactgt tcctgctagt tgcacaggtt ctaaatttgc
     2761 tactggagta acagccccag ttctaccaac ttgataatca atacttaaaa gttttgtgct
     2821 aacttgctct gccttaaatt tatacgcaat tgcccaacgt ggcgattttg ctgtaaatcc
     2881 taaaatttct tgttgtgcaa acgaatttac ttttattaca attccgtcaa tatcataact
     2941 taaatttttt ctttcctcgt cccaaaattc aatataatta taaacttcag aaattgtttt
     3001 gcaaagttta ctatgttccg aaattttaaa tccccaagat tttagttgtt gtaaactttc
     3061 ccaatgagtt gtagcagaaa gatttttgcc catcatataa taaaaatagc aatctaaatt
     3121 tcgtttagct acttcgcgag aatcaatcat ttttattgag cccgatgttg catttcgtgg
     3181 gtttgcaaaa ggagattctc cattttcttc acgaatttca ttcaattcat caaaaacttt
     3241 gtgaggcata attatttcgc cacgaatttc aaaattttga ggaatatttt cgccagaaat
     3301 agaaagtgga atacttctta tagtttttat attttcgcta acatcatcac cttgagtgcc
     3361 atcgccacga gtaaccgctc tcaagtattt tgaattttca taagaaactg aaatcgaagc
     3421 tccatcaaat tttaactcgc aaacatattc tgggtcaatt tctatttctt tcacaattct
     3481 attatgaaaa tcattaagtt cttcagcaga atatgtgttt cctaaagaca acataggaaa
     3541 ttcgtgagtt acagttttaa aaacattgga aatatcactt cctactctct gagttggact
     3601 gtcggcttgc ttaaattgtg gatatttgtt ttctaaatct attaattcag ccaaaagttt
     3661 atcaaattca aaatcgctaa tgcttggatt atttaataca taataattat gattatgttt
     3721 gtttaattct tcgctaagct ttttaatcct caattcaata ttttccatag ttaaaattta
     3781 tgttttgaca aatataaaaa atattaaaat atgtttgtga ttttttaatt aacttagtag
     3841 cttaaaaatt taattatgaa aagactattt tccatacaat ttttattgag catattagtt
     3901 ctaacagttg ctataacttc ttgttcagaa aataaaaaat ctaaaaaact agacgaacaa
     3961 caaattttag aacaaaataa tattgaagtt gctaaaaaac ttgtgaacga tttttttgat
     4021 ttgctaaata aagaaaacta tgaaacttgc tattctcatt tttcggattc tttaaaacaa
     4081 tattttagta aagaaaatct tatcacaact ataaaagcaa gaaatactga gtttccaaat
     4141 tctgatagtt taggaattta ttgtactcaa gtgttaacaa cagaaatagg cgatgtttac
     4201 tttttttatt atcgtgtgtt ttcaaaatca aaattgatat tttatgaaaa aatcagtttg
     4261 tattttcaag aaggtttacc ccaaatttta ggctacgatt attcagaaaa tattttttac
     4321 actccaaaaa tggcaaatga cgctgattct gatttaaaat tagttttaca aaacatgtat
     4381 caaatcttaa atagtggaga cattgaaaat gcttatgcca tggttgatga gactgtgaaa
     4441 gagaaattaa gtttttcaaa atttgaagct ggatttaacg aaaagaaatc aaaatatcaa
     4501 ggtactaatt cattttctgt aaaatcgatt tggacagaaa ttttatatga tgttcctgtt
     4561 ttaaacttga ttgttgatgt tgtaaattat agtggcgata gatttttaga taaaattatg
     4621 atttcagata gagatggaaa gttttttatt gcaactatag atagagaaga ttttgaaaaa
     4681 gcagaattaa taaaaattga aagacttcca gatgcagaac ttaataaata tgccgaagtt
     4741 gcttctaaat tttatgaatt tttaaaaaat tcaaattttt atgaaataat gcaaatgctt
     4801 gatagaacag tttttataaa taatgactat gaaacaataa aatcatcgtt ttcggctaga
     4861 gagcaatatt atggtaaacc taaaaattta aaaaatactt tagtaaaaac ttactctttg
     4921 ggaagtaaaa ctttagtaga atttaatttt gatgttgaaa acacaaatgg aaaaatttcc
     4981 tacgaaaaaa ttatagtttc taaaactaca gaagataaat attttatttt ttcttacgat
     5041 tttagagata ctagattttg aaaaaataat aaatcaaagc tactctacta cttttttacc
     5101 ttctttatac atttcttctc ctatcatatt tcctttatca tcgtagaatt gccacaagcc
     5161 atctgcaagg tcgtctttat agcttttcac atatctaact tttccatttt cgtaaaacac
     5221 ctcgctcctg ccctgtttta gaccattttc ataatatcca atactccaaa ctactccatt
     5281 ttgataatat gctgtccatt ttccttctct tttaccatca acaagcaatc cttctataaa
     5341 taagtttcca ttctcataat attgcttttc ataagcttgt ttttcgtctt ctttatcaac
     5401 aaaatgtact tttaatgggt tttcgttttc aaaactttca acaattttca catctgacga
     5461 ctgtgccaaa gcatcgtgtt ttatgatagg attgtcttgt tgttttgatg ttacttcttc
     5521 aacttttgac ttatctttac acgaagctac gccaagtgct gcaaggatat agacaaataa
     5581 aacagcaatt ctaaaaactt tcatatttca taaattttaa tccgccaaat ataaataaaa
     5641 gctaagattt tttcaagtgc tttctcaaga ttttcattaa acatgaaatt taatctcaaa
     5701 aattttcaaa attcttaaca agattgtatc tttgtcaaga tttttacaat tgagaattat
     5761 ttagaacttg atatatggta aaacaaacga ctatttcaaa agaagttaaa attgagggcg
     5821 tgagtttgca tacaggaaaa ccagcaactc ttttgataaa gccggcagag ccaaacacag
     5881 gctacgtatt cgtaagaaca gatattgaaa ataatcctaa gattcaggct gttgcagaaa
     5941 atgttgttga tacttcaaga ggtacaacta ttggtaaaaa tggtgttaat gttagtacaa
     6001 tagaacattt attagcaact acttacgcac taggaattga taatgttata tttgaaatta
     6061 acggaccaga agttcctatc ttagacggaa gtgcaaaata ttttattgag gcttacaaaa
     6121 acacagaaat tatagaacaa gatgcagaac gcatagtttt taatattgaa gaaaatttat
     6181 catttgttga tgaagaaaaa aatatagaaa tttctgttta tcccgacaaa acatataaac
     6241 ttcaagtttt agtcgattat aaatcagatg ccatttcaaa tcaatatgct gttttaaata
     6301 gtttagacga gtttaaaaat gaagtttctg cttgcagaac ttttgtattt ttaagcgaat
     6361 tagaaccact attaaaacaa gatttaatta aaggtggcag tcttgataat gcaattgtaa
     6421 ttgttgataa agaatgtgac caagcagaat ttgaccgttt ggctaaatta tttaatagac
     6481 caagtgtaga agttcaatct tctggtgttt tgaataatat aaaattacaa tttagtaatg
     6541 aacctgcacg ccacaaactt ttagatttaa ttggcgattt agcactaaca ggctatagat
     6601 ttaatgctaa tataattgca aatcgtccag gacattatgc taatgttaat attgctaaaa
     6661 taattcgtca acatcttaac gaggtaaaga aaaacttagc gaaagcaaat attccaattt
     6721 acaatccaga cgaaaaacct ttgatggatg taatgcaaat tcaaggtttt atacctcatc
     6781 gcttcccatt tttgttggtt gataaaattt tatctttaag cgaaaccgaa gttgttggtg
     6841 ttaaatgtat tactaatgac gaaaactttt tccaaggaca tttccctgac gagcctgtaa
     6901 tgcctggtgt tttacaagtt gaagctatgg cacaaactgg cggaatatta gttttatcta
     6961 cagttccaga ccctgaaaat tatcttacat ttttcttaaa aattgacaat gtaaaattca
     7021 gacgtaaggt agttccaggc gatgttttag taatgcaact aactttatta gaacctattc
     7081 gtagaggaat agcccacatg ctaggcaaag cttatgttgg cacagaactt gcgtgtgaag
     7141 cagatatgta tgcgcaaatt gttaaactta gttaaaaaaa tattatgaat caacctcttt
     7201 cttttgttca ttcggcagca aaaattgcca ataacgttgt ggtagaacct tttgttacta
     7261 ttcacaaaaa cgtagaaatt ggagaaggaa cttggatagg ttccaatgtt acaattatgg
     7321 aaggctcaag aattggtaaa aattgtagaa tttttccagg tgctgttatt ggagcaattc
     7381 ctcaagacct aaaatttaga ggcgaagaaa ctaccgctga aataggtgac ggaacaacta
     7441 tacgtgaatt tgtaacagta aatagaggaa ctgctgcaaa aggaagaact attgttggtt
     7501 caaattgttt gataatggca tatgttcatg ttgctcacga ctgccttatt ggtgataatg
     7561 taatactagt aaatggtgta aatttaggtg gcgaagtaat agttgacgat tatgcaatta
     7621 tttctgcgaa ctctttaatt catcaattct gtagaattgg agcacataca atacttgctg
     7681 gtaattcact agtaggaaaa gacgtgccac cttatgttaa agctgctagg tatcctcttt
     7741 cttttgcagg agtaaattct attggcttga gacgtagaaa tttctgtaac gataaaatta
     7801 atgctattca agatatttat agagtaattt ataatcgcgg aatgaacgtt tcacaagcta
     7861 ctgcttatat tgaagcggaa atgcccgcta gcaaagaaag agacgaaatt ttattattta
     7921 tccgaaactc aaaacgcgga attattaaag gctattttga taaaagcgaa tctgacgtag
     7981 cgttttaaac taagctcaat ttttttaact taatctctat tccaaatttc ccaagctcgg
     8041 tctgcttgtt cgtatagcat agttaagcca ttaatgcatt ttgcgccttt gttttcacaa
     8101 tttttcaaaa ataaagtttg ctcaggatta taaactaaat caaagcaaat atgcgtgtct
     8161 ttaatatatt cataaggaat atttggacaa ttatctacat ttggaaacat tccaactgga
     8221 ctagcattaa taattaaatt gtgatgctta ataactgcct ctgtaacgtt tttataagat
     8281 aatacagaca agttttttgg atttcgtgaa acagtagtcg atttaattcc aagttcttct
     8341 aaaacaaatt ttacggtttt gccagatcca ccacttccta aaattaatgc tttatttatt
     8401 ggtggaagtt ctattttttc aaaagtctta cgcaaaccat caatgtcagt attataacca
     8461 gttaatttta tttcttctgc gtttctttca atttttatag tattaacagt accaatattc
     8521 aaaacggatt tatcaatatc gtttaaataa ggcaatatct gttctttata aggagttgta
     8581 acgtttaagc ccaataaatt ttctttagta cttataatat tatgtaactt agaaatctct
     8641 gttataggaa ataaactata ttttgcgtca tcaatttttt catttttaaa tttgttctca
     8701 aaatatatat gagaatatga atgtttcaaa ggataaccta taagtccaaa ctctctcatt
     8761 tttttctttt gataagttta cgaataataa aaaataaaat aactaaaaca acaattacaa
     8821 ctatagctat tgataattct ttattgtatt tatctattaa atctttttgt ccatgagcta
     8881 aataacctaa agctgccaaa ataatattcc aaatcatagc tccaataaaa gtatatatgc
     8941 taaaagcaag aaaattcatt tttgccaatc ctgctggaat agaaattaat tgacgaattc
     9001 cgggaattaa tcttcctata aaagtagagg ttttcccgtg attgttaaaa tatgtttctg
     9061 cttttacaac cttttcctta ctaagtaaaa agaagcgtcc caagcgagaa tctgcaaatt
     9121 tataaactat aggtcgcccc aaccataatg ccaaaaaata attaatataa gcacctatca
     9181 aagctcctat ggttccaaat aaaacaacta aaaatatatt caaatcgctt tctgggtcgc
     9241 ttgcaatata agcagctgga ggaataacaa tttcggaagg aaaaggtata aatgaacttt
     9301 caatagtcat caataaagta atagttgtat aattcatatt ttcagaatac caattctcta
     9361 ctttttttag caaggaaaga ttttctgtac tgtcaacagt attcgctttt aaagaaacgg
     9421 tacaagctaa aattacaaat attaaaattg attttaatac ccttgtcttc atctattttc
     9481 aaattttgca cgaagtaaca aatttattag tatattgcaa agcggttttt ttcttagtta
     9541 ttaacaaagc aattagaatt tctccaaaga ttttaaattt tcctattttc aaagttgtag
     9601 cacccacgcc gacaaatcag ttaaaagtct aaagtgtttt tagttttgag tgttgagttt
     9661 ttagtgttga gttttaagtg ttgagttttt agtgttgagt ttattgctca gatggattta
     9721 actttacgaa ctttaaaaac tttattaact tttaactgtg tttattgtgt tgccctacga
     9781 gcaaactttt gataaagtga tgaaagttaa ttacgaaaaa ttgatgaaaa attaatttgc
     9841 tatttacaaa acctacacca gctcgcttct agcaccacta ttgctcgctc ccaacaccca
     9901 tgacgctcgc tcggatttgt aatccgagtg taacattatt cagcactcgg attacaaatc
     9961 cgagcgagcg ggagtgttta ctgtgttgcc cttgcgggca aggctttcga gggtttatat
    10021 accgcaggct gcgcctacgg ttaatcacat gttgtcctac ggacaaacgc ttgatagtct
    10081 tgttttgtcc ttacggacaa acaaattagg tattccacaa aaaaacaaca aatcacactt
    10141 gattatcaga gacttggtaa ctgtcgcaaa aaaagtgcgg aaaacaagaa aatacaaatg
    10201 gaaaaatttc ttacgaaaaa attataattt ctaaaactac agaagataaa tattttattt
    10261 tttcttacga ttttagagaa aacaggtttt agaaaaatga taaatcaaaa ctaatctact
    10321 tttttacctt ctcctgtttt caacgttgta gcacctatgc cgacaaatca gttaaaagtc
    10381 taaaatgttt ttagtgttga gtttattgct cagatggatt taactttacg aactttaaaa
    10441 actttattaa cttttaacta aaattttctt gctttaatat gtttcgcttg catgataatt
    10501 gaaaatttaa tttaactttg tcattattaa aattaataaa taatattcac tatggaacaa
    10561 tcaaaacgac aattacaggt tggcaattta gtacaaaaag aattatctca tattattcaa
    10621 cataaattta atggtttagt gccaggcaga ttgctaacag ttacaaatgt taaaatgaca
    10681 ccagatttaa gtattgcccg aattagttta agtatttttc cttcaaacga tggcgaagct
    10741 attattaaag aaataaacaa acaggtgtcg cctattagat ttgaacttgg caatgctctt
    10801 agaaataatc ttaaaaaaat tccagaattg gttttttact tagacgattc tctagaccaa
    10861 agtgaaaaaa tagatcaatt attaaaaaag taaaatttga aattttcttt ttacatagct
    10921 cgccgttact tgatttctaa aaaatctaga aatgcaataa atattatttc attcatttca
    10981 gttttaggag ttagtgttgg cacagctgct ttaattacta tcctatctgt atttaatggc
    11041 tttgataatt tactaacaag gctttacaat tcttttgacc ccgatattaa aattgttgca
    11101 gcaaaaggca aggtttttaa atttgatgat aaagtttctg aaaaactaaa tttagatttt
    11161 atcgaagaat attcgctttg tttagaagaa aacgcaatgt ttgcttacga cgacaaacaa
    11221 attgttgcaa caataaaagg agttgacgat aattataaaa atgtaacagg catagattct
    11281 atgattgtta gaggcgatta ttttttagaa tatcaaaatg ccttttgcgg tgtttcgggt
    11341 caaggaatag cctataattt gaatttagat gtaagtttta tttatcctgt aaaagtttat
    11401 attccaagca gaacaaaaaa aattagtgct ggcatgatag atgcagcaga caatctaaat
    11461 caaagtattg tttataatgc tggaattttt tccattcaac aagagtacga cactaaatat
    11521 tttattgcac ctctttcgga agttagaaga cttttagagt atgaaaatga ggtaagttcc
    11581 atagaaatta aaattaaaaa aggcgaaaac ataaaaactt cattaaataa actaacagaa
    11641 agttttggcg aagattttat tgttcaagac agaaatatgc aacacgaata tgcatttaaa
    11701 ataatgcaat cggaaaaatg ggcaattttt atgattttga ttttcattct gttaatagca
    11761 tctttcaata ttattggttc tttaacaatg cttataatag aaaagaaaaa tgatattaaa
    11821 attttaaaaa gcctaggagc agaagacaaa actattagaa aaatattttt cttacaagga
    11881 ttatttatca gttttatagg tgcaattgtt ggaatgctgt ttggaagtgt tctttgttgg
    11941 gctcaaataa aatttggttt aataaaacta ggtactggag gttcgtttat aattgatagc
    12001 tatcctgttg caatgcaagc aatggatttt gtctgggtat ttattgctgt aatgattatt
    12061 ggacttatag ccgcttggta ccctgtgaga tatattacga aaaaattctc ggaagtcagt
    12121 taaaaaa
//
LOCUS       DAOPNX010000078        11932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_6185_length_11932_cov_7.283910, whole genome shotgun sequence.
ACCESSION   DAOPNX010000078 DAOPNX010000000
VERSION     DAOPNX010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11932
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6185_length_11932_cov_7.283910"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1094)
                     /locus_tag="PKN53_08600"
     CDS             complement(54..1094)
                     /locus_tag="PKN53_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOD89046.1"
                     /translation="MIDNRKQQIIDIVKEKKQCSSKEIYDSIGDSFSYSTLKRILTDL
                     ITDNYLLTKGQGKGTKYLLSPAYELICPIDIEKYFEKEIDERIIIKNFNINIITEVLD
                     KYNLFTEGELKKLATLQKEYQENISQLSENEYKKELERLAIDLSWKSSQIEGNTYTLL
                     ETERLLKEKETAYGKTKEEAVMLLNHKDALDFIIEHKDYLSPLTVSKIEDIHSILIKE
                     LAIEKNLRKRRVGITGTNYKPLDNEFQILEALKKACDVINRKESVFEKALLALVLISY
                     IQPFMDGNKRTARLVSNAILMNYGYCPLSFRTVDSIDYKKAMLLFYEQNNISNLKNIF
                     IDQFEFAVGTYF"
     gene            complement(1393..2019)
                     /locus_tag="PKN53_08605"
     CDS             complement(1393..2019)
                     /locus_tag="PKN53_08605"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 domain-containing protein"
                     /protein_id="HOD89047.1"
                     /translation="MLHQIELNFYERHGKAITNFTETLPSPHSELATETLKDPYKFDF
                     LTLQEKALEKDIEKQLVKHITSFLLELGTGFSFVGQQVPVKIDNQDFYIDLLFYHIKL
                     KCYVVVELKAIEFKAEFAGKMNLYLSAIDDSLKTEAENPTIGLLLCKSKSKIIAEYAL
                     RGMTQPIGIAEYEISNAIPKEIKTELPTIEEIELATTKAIRKAGDSNK"
     gene            complement(2074..2451)
                     /locus_tag="PKN53_08610"
     CDS             complement(2074..2451)
                     /locus_tag="PKN53_08610"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HOD89048.1"
                     /translation="MSLTKKHTEYINWVNELKTLIQRTQIKASILVNRELMSLYWTIG
                     KSISEKVNTANWGSSVVEKLSKDLKEEFPNQKGFSRSNLFSMKKWFEFYSQSDIDTEK
                     IQQLVGQIPWGHNVVIISKSKNH"
     gene            complement(2710..3828)
                     /locus_tag="PKN53_08615"
     CDS             complement(2710..3828)
                     /locus_tag="PKN53_08615"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOD89049.1"
                     /translation="MKRILLFVILIFLSFFTFSQKYSFIEGKTSIGKFVPHHRIMNEL
                     IDKTSFGFEVNYLTKNYSDKCYNKKYKFPYKGFGLSFYNLGNPEVFGYSFSAYGLMEF
                     NLAKTPNFEFNIRSSSGLSYLTEKYDKDKNPYNVAIGTNLNYLFTLGFNASYQLGNYS
                     LLRLGADFMHNSNGSTRKPNLGVNQVFLSLSYSQLLLEKSEFKYSEYSRERLSPHEIY
                     TFISFCTSDEYKEIPESRGGGFLCSSHSLAYNYQYSPIGKVGVSHDIFYDENLRYYYD
                     RERDSLMMRTDKFSDILKYGISFGHQLIFNRLELTTFAGFYYYQQVKKTENFYTRIGA
                     RYYLSDYLFINLTLRAFGFKAHFIESGIGFSFRKAKDN"
     gene            complement(3836..7048)
                     /locus_tag="PKN53_08620"
     CDS             complement(3836..7048)
                     /locus_tag="PKN53_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOD89050.1"
                     /translation="MKKFLVFCFISFLSFSLFSQNAETRLMRFPDIYGDKIVFSYAGD
                     LYLVSDQGGLARRLTSHEGLEIFPRFSPDGKHIAFTAQYDGNSEVYIMPAEGGIPKRI
                     TYTACLNRDDISDRMGPNNIVMTWTNDSKNVVYRTRGKSFNDFKGQLFLAPVDGGMSE
                     QLPLAYASWCSYSPDGNKLALNQVFREFRTWKYYKGGMADDIWIFDFKTKKIENITNN
                     VAQDIFPMWHENKIYFLSDRDRTMNLFVYDLNNKNIEKLTNFTDFDIKFPSLGKGYIV
                     FENGGYIYKFNLNDNKLTKLQIQIQEDFANSRNIIKDVSKSATNVHISPDGSRLTLSA
                     RGDIFSVPAKNGITKNLTQSSNAHDRNPVWSPDGKYIAYLSDKSGEYQIYIQEPDKVN
                     SEKQLTNFEKGYIYKLVWSPDSKNLLWNTKSLELNCINIETKAITTIHKSIAREIYDY
                     TWSPDNNWVAFSDEDENRVLQIYLYNIKDKKEYKVTSQWYNSYSPHFSHDGKYLFFIS
                     NRDFNPIYSAVEWNIAYQDMSRIYFLTLNSDIKSPFANENIEVFGKDTAKKDDKAFNL
                     EIKNIENRIAALPMLAGNFRNLNYVNGKLYFVESRRGQSSKFKYYDLNEKKEQEIGDY
                     WNYQISADRKNVLVYNNGNWYISNLPTGSLSLKESVSFSDLKINIDLKAEWQQIYFEA
                     WRQMRDFLYDPNLHQVDWEKQKNKYAELLPYVNSRNDLNYIIGELIGELNVGHAYVGG
                     GDRKEIQRIKTGLLGAEFEKDKSGYFKITKIYQGENWRNNLTSPLTGIGLDINENDYI
                     TSINGQDLKEVDNIFSVLIDQADKMIEIEVSTSSNGKNAKKYFVKPISDEADLIYFNW
                     VRENIRKVNEATDGKVGYIHVPDMSVAGLNEFMKYYYPQLSKQALIIDDRGNGGGNVS
                     PMLIERLRRELSIMGMQRDVPLTNMKPRGMLFGPKVLLLDNYSASDGDLFPYQFKRHE
                     LGVTVGQRSWGGVVGIRGSLPFIDGATLSKPEFAHFDENGWIIEGYGVDPDVVVENDP
                     YQEFLGNDQQLNEAIKIILQEIEKFGKTKPAIPDFPDKSK"
     gene            complement(7384..8172)
                     /locus_tag="PKN53_08625"
     CDS             complement(7384..8172)
                     /locus_tag="PKN53_08625"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOD89051.1"
                     /translation="MARIITFTTDWSSSDYYIGAVKAAIISKCSDVSFIDISHNIEHF
                     SVFQAGFVLKSVYKNFPEGSIHIIGVDSEPDRDGYILVSKYDSQYFISSNNGCTGFIF
                     EKEPEIAVLVETGFAFEGASFIELNLFSHIAAFIVRDGDITELGEPINKIKRKLSVNC
                     QIETDFINGEVIYIDSYQNAITNISREIFKDYVGDKKFEILINTNNITVSEIKQSYKE
                     VDSGEIVCIFNSLGLLEIAIRDGKATQLLNLGIRSEIRIKYNFS"
     gene            complement(8296..8979)
                     /locus_tag="PKN53_08630"
     CDS             complement(8296..8979)
                     /locus_tag="PKN53_08630"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOD89052.1"
                     /translation="MKKIVVLSGAGISKESGIPTFRDSDGLWNKYRFEEIASPEAWKA
                     NPQLVLDFYNFRRKVVLDSKPNKAHLTLKELDKNFDVKIITQNVDDLHERAGSTDVLH
                     LHGEIRKARSTKDENIIVDIQGSELNLGDLCPLGSQLRPHIVWFGEAVPNISHAIDIV
                     KTADIFLVIGTGLTVYPAAGLINYLPKKSQNFIIDPSDDFYGINNFKHIQKSAVNGID
                     EFVQAIKTF"
     gene            complement(8984..10255)
                     /locus_tag="PKN53_08635"
     CDS             complement(8984..10255)
                     /locus_tag="PKN53_08635"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HOD89053.1"
                     /translation="MKKFLFLLSFAILASGISAQDVKSHKPMSKFSYKIYTKRISKPR
                     KTEEKRRIKAVKLLRWKHISSNQLYKASLIIQDEGQRLVYVKEAYPKITDKSNALMLT
                     NAFEKYLHVQLLWEFIEKNNEQLGIAPMDLESYTKYLSLKEKKKDKQRDEIAKNENKD
                     KPKDKTEPVKPQEPQVVEKPETPVQTDKEETKPTEPSSTNSIVYPKAEGYTGSKGCES
                     CLNEQQFLAFKNALSRYETDEEKTKICMEYVYTYCFSTAQVMQLAELIKNEKMRFVFL
                     KTAYEKVYDRDNYLHVKQLLITQSYIHEINSIYQVPKPEKPYAVGIPAPCTLSDNDYE
                     KIKSDVRKEMSSTQKLEKAKNLIPQYECISAQQVKSMLNLFSLENDKLDFAKFAYKYC
                     NDRDNYSVVSSFFTSINSRNSLNEYIKSYKD"
     gene            10891..11061
                     /locus_tag="PKN53_08640"
     CDS             10891..11061
                     /locus_tag="PKN53_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89054.1"
                     /translation="MNFFYETKDCPLPEDNSDIVFTDLATELIEKIQSFKIFYKTFIE
                     PELKSIAYEFLR"
     gene            11209..11790
                     /locus_tag="PKN53_08645"
     CDS             11209..11790
                     /locus_tag="PKN53_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89055.1"
                     /translation="MGFNILNYSSIDIPSDPPQPKFDISFINGIYFKRHYNENTLRLS
                     VDQLTCNVKIHGNGSYEADTLITMGNWREQMFSMGFEQNYFEKKQFHLYWGVDLNLYY
                     GIYSGQSQSLSGLSNFSTVKQNKGIGLTPLAGIRFTISNFLRISLETSGSSLFFTEFE
                     NKKWTLPMVKNEQNQAITFALKYNVGCKISYLF"
BASE COUNT         3819 a         1884 c         1685 g         4544 t
ORIGIN      
        1 ttttttaaaa aatgcatgtt tttttatatt gtgttgtagc ttgtcttttt atcttaaaaa
       61 tatgttccaa cagcaaattc aaattgatct ataaaaatat tcttcaaatt agaaatattg
      121 ttttgttcat aaaatagtaa cattgctttt ttataatcaa tagaatccac tgttctaaat
      181 gataaaggac aatagccata attcattaag atagcattac taacaagacg tgccgttctt
      241 ttatttccgt ccataaaagg ttgaatgtaa gaaatcaata ctaaagcaag taaagctttt
      301 tcgaatacac tttcctttct atttatcaca tcgcatgctt ttttcaaagc ttctaaaatt
      361 tgaaactcat tatctaaggg tttataattc gttccagtta tacctactct tctttttctt
      421 aaattctttt caattgctaa ttcctttatt aaaatactgt gaatatcctc tatttttgaa
      481 actgttaatg gacttaaata atctttatgt tctattataa aatcaagtgc atctttatga
      541 tttaatagca ttactgcttc ttcttttgtt ttaccataag ctgtttcctt ttcctttagc
      601 aatctttcgg tttctaaaag agtatatgtg tttccttcta tttgtgatga tttccaactt
      661 aaatcaatag caagtctttc taattccttc ttatactcat tttctgatag ttgagatatg
      721 ttttcttgat attccttttg taatgttgct aattttttca attctccttc agtaaataaa
      781 ttgtatttat cgaggacttc tgtaattata ttaatattga aatttttaat tattattcgc
      841 tcgtcaattt ctttttcgaa atacttttct atatcaatcg ggcaaataag ttcataagct
      901 ggagataaaa gatactttgt tccttttccc tgtccttttg tcaataaata attatcagtt
      961 attaaatctg ttaggattct ctttaatgtt gaataactaa aagaatctcc aatgctatcg
     1021 taaatttctt tagatgaaca ttgtttcttt tcttttacaa tatctatgat ttgttgtttt
     1081 ctattgtcaa tcatttttta tttttaagct cacaaatata ctgtttttag ttcaatattt
     1141 gatatttttg agctgtatat ttcattcaaa tgagccacaa cgacaaattt acctaagaaa
     1201 tatacaacat tcgtcctata aatcaattat atacagttac aaaattaatt acaaatattt
     1261 gcaattgcga atgatttgtt gaatttttta aaaatggttg tttttcattt ttaatggtat
     1321 gggctatgtg gagtgtggat tttaaagtac tttactttca atttattgct aaattttata
     1381 ctggaaatct ttctatttat tactgtcgcc agctttacgt atagcttttg ttgtagccag
     1441 ttctatttct tcaattgttg gtaattcagt ttttatttct ttcggaattg cattgctaat
     1501 ttcatattct gctattccaa taggttgtgt cattcctctt agtgcatatt ctgcaattat
     1561 tttagattta gatttacata acagtaatcc aatagttggg ttttctgctt ccgtttttaa
     1621 actatcatct attgccgaca aatacaaatt cattttgcca gcaaattccg ctttaaattc
     1681 aattgctttt aattcgacca caacatagca ctttagctta atatgataaa aaagtaagtc
     1741 aatatagaaa tcttgattat caatttttac aggaacttgt tgtccgacaa atgaaaatcc
     1801 agttcctaat tcaagtaaaa aggaagttat atgtttaact aattgttttt ctatgtcttt
     1861 ttcaagtgct ttttcttgta atgttaaaaa gtcaaatttg tatgggtctt tcagagtttc
     1921 tgttgctaat tcagaatgtg gggagggtag tgtttctgtg aaatttgtaa ttgcttttcc
     1981 atgtctttcg taaaaattca attctatttg atgcaacaaa actgaacgtg accaattgtt
     2041 ttcatcgttt tgtttgaata aaaaatagct tcttcaatgg ttttttgact ttgaaattat
     2101 cacgacatta tgtccccatg gaatttgtcc aacaagttgt tggatttttt cagtatctat
     2161 atctgattgt gaataaaatt caaaccattt tttcattgaa aacaaattgc ttcttgaaaa
     2221 acctttttga tttgggaact cctcttttaa atcttttgac aacttttcaa caaccgaact
     2281 cccccaattt gcagtattta ctttttcaga aatcgacttg ccaattgtcc aatacaaact
     2341 cattagttcc ctattaacca aaatagatgc ttttatttga gttctttgaa ttaaggtttt
     2401 tagttcattt acccagttaa tgtattctgt atgttttttt gtcaaactca taattacaaa
     2461 aatatatcaa ttttttcaat ttttctaaaa aataatggtt tcaatattag gagtcggttt
     2521 tcttgaattg gctacaacat atattttacg caccaaatat tcagcattcg taaattattt
     2581 ttataattta atttaatcac aaagctaatt aaaatttttt gcatttgcaa aattatttgc
     2641 taactttttt taacctgcag tttgagcgag gtagcttgtc cgtaggacaa cacaattaat
     2701 tatcaattat caattgtctt tggcttttct aaaactaaat ccaattcccg attctataaa
     2761 gtgagcttta aagccaaaag ctctaagagt taagtttata aaaagataat ccgaaagata
     2821 atatcttgca ccaattcttg tgtaaaaatt ctctgttttt ttcacttgct ggtaatagta
     2881 aaaccctgca aaagttgtca attctaatct gttgaaaatt agctgatgcc caaagctaat
     2941 cccatatttt aaaatatcgc taaacttatc tgttcgcatc attaagctat ccctctctct
     3001 atcataataa tagcgtaaat tttcatcata aaaaatatcg tgagaaaccc caacttttcc
     3061 tattggcgaa tattgataat tataagcaag tgagtgagaa ctgcataaaa atccgccacc
     3121 tctgctttcg ggaatttctt tatattcatc agatgtgcaa aaacttataa atgtgtaaat
     3181 ctcatgcgga ctaagtcttt cccgcgaata ttccgaatac ttaaattctg atttttcaag
     3241 caataactga ctgtatgata aagatagaaa aacttgattt actcctaaat ttggttttct
     3301 tgtcgaacca tttgaattgt gcataaaatc agcacctaat cttaataaag aataattccc
     3361 caattgatat gaagcgttaa atcctaaagt aaataaataa tttaaatttg ttcctattgc
     3421 aacattgtag gggtttttat ctttatcgta tttttccgtt aggtaagata agcccgaaga
     3481 acttctaata ttaaattcaa aatttggagt ttttgctaaa ttaaattcca taagtccgta
     3541 ggcagagaat gaatagccaa agacttcggg atttcctaag ttgtaaaaac ttagcccaaa
     3601 gcctttgtat ggaaatttgt attttttatt atagcattta tcactgtaat ttttagttaa
     3661 ataatttact tcaaagccaa agctggtttt gtctattaat tcattcatta tcctgtgatg
     3721 aggcacaaat tttcctatag aagttttgcc ttcaataaaa gaatattttt gcgaaaatgt
     3781 aaaaaatgat aagaaaatta atatgacaaa taataatatt cttttcattt attttttatt
     3841 tagatttatc tggaaaatct ggaattgctg gttttgtttt tccaaatttt tcaatttctt
     3901 gaagaattat tttaattgct tcgttcagtt gttggtcgtt tcctaaaaac tcttgatatg
     3961 ggtcgttttc tacaactacg tcagggtcaa cgccatagcc ttcaattatc caaccatttt
     4021 cgtcgaagtg tgcaaattct ggtttagaaa gtgttgctcc gtcaataaat ggtaaacttc
     4081 ctctaattcc tacaacgccg ccccaagagc gttgacctac cgttacgcca agttcgtgtc
     4141 ttttaaattg ataagggaac aaatcgccgt cagaagcaga gtaattatct aataacaaga
     4201 cttttggacc aaataacatg cctctcggtt tcatgtttgt aagtggcaca tctctttgca
     4261 ttcccataat agaaagttct cgccttaatc tttctatcaa cattggactt acatttccac
     4321 cgccatttcc tctgtcgtca ataattaagg cttgttttga taattgtgga taataatatt
     4381 tcataaattc attcaatccc gcaaccgaca tgtcaggcac atgaatatat ccaactttgc
     4441 cgtctgttgc ttcgtttact tttcttatgt tttctctaac ccaattaaaa taaattaagt
     4501 cagcttcgtc agaaattggt tttacaaaat atttcttagc attttttcca tttgagcttg
     4561 tgcttacttc tatttcaatc attttgtcgg cttggtcaat taaaacagaa aaaatattgt
     4621 caacttcttt taaatcttgt ccatttatgc ttgtaatata gtcgttttcg ttaatgtcta
     4681 atccaattcc agttaaaggc gaagttaaat tattacgcca gttttcgcct tgataaattt
     4741 ttgtaatttt aaaataacct gatttatctt tttcaaattc agcacctaat aagcctgttt
     4801 taattctttg aatttctttt ctgtcgccac cgccaacgta agcatgacca acatttagtt
     4861 cgccaataag ttctccaata atataattca aatcatttct cgagttaaca taaggcaaca
     4921 gttctgcata tttatttttt tgtttttccc aatcaacttg atgtaaattt gggtcgtata
     4981 aaaaatcacg catttgtcgc catgcttcaa aataaatttg ttgccattcg gcttttaggt
     5041 ctatgtttat ttttaaatca gaaaaagata cagactcttt taaacttaaa ctgcctgttg
     5101 gtaaattgct aatataccaa ttgccattat tatataccaa aacatttttt ctgtctgcag
     5161 aaatttgata attccaataa tcgccaattt cctgttcttt tttctcattc aaatcgtaat
     5221 atttgaattt tgaactttgt cctctacgac tttcaacaaa atacagtttt ccatttacat
     5281 aattcaaatt tctaaaatta cctgcaagca ttggtaaagc agctattcta ttttcaatat
     5341 ttttaatttc taaattaaaa gctttgtcat ctttttttgc agtgtcttta ccaaaaactt
     5401 ctatattttc gtttgcaaaa ggcgatttaa tatcagaatt taaggtcaag aaataaattc
     5461 tactcatatc ttggtaagcg atattccatt ccacagctga ataaatagga ttaaaatcgc
     5521 gattagaaat aaagaataaa tattttccat cgtggctaaa atgtggcgag taagagttat
     5581 accattgaga tgtaacttta tattcttttt tatctttaat attgtataaa taaatttgca
     5641 gaactcggtt ttcgtcttcg tcagaaaaag ccacccaatt attgtctggc gaccaagtat
     5701 aatcataaat ttctcttgca atagatttgt gaatagtggt aattgctttg gtttcgatat
     5761 ttatacaatt taattctaaa gattttgtat tccacagcaa atttttactg tcaggcgacc
     5821 aaactaattt atagatatat cctttttcaa aatttgtaag ttgtttttcg ctatttactt
     5881 tgtcgggttc ttgaatataa atttgatatt cgcctgattt gtcagaaagg taagcaatat
     5941 attttccatc tggcgaccaa acaggatttc tatcgtgagc atttgaagat tgagttaagt
     6001 ttttagtaat tccatttttt gcaggaacag aaaaaatatc gccccttgcc gaaagagtta
     6061 aacggctgcc atcaggagat atatgaacgt ttgtagccga ttttgaaaca tctttaatta
     6121 tgtttctgct attagcaaaa tcttcttgaa tttgaatttg taacttagtt aatttgttgt
     6181 cgtttaggtt aaatttataa atatatccac cattttcaaa aacaatatat cctttaccta
     6241 aacttggaaa ttttatgtca aaatctgtga agtttgtaag tttttcaatg tttttattat
     6301 tcaaatcata tacaaataaa ttcattgttc tgtctctgtc tgagaggaaa taaattttat
     6361 tttcgtgcca cattgggaaa atatcttggg caacattatt tgtaatattt tcgatttttt
     6421 tagttttgaa atcaaaaatc catatgtcgt cagccattcc gcctttgtaa tatttccaag
     6481 ttctaaactc tctaaacact tgatttagag ccaatttgtt tccgtcaggc gaatacgaac
     6541 accacgaagc ataggctaat ggaagttgtt cgctcattcc gccatcgaca ggagctaaaa
     6601 aaagctgacc tttaaaatcg ttaaaagatt ttccgcgagt tctgtaaacc acgtttttac
     6661 tgtcattagt ccatgtcatt acaatattgt ttggacccat tctatcagaa atatcatcac
     6721 ggtttaaaca agcggtgtaa gtaattcttt taggaattcc accttcggca ggcattatat
     6781 aaacttctga atttccatcg tattgagctg taaacgcaat atgtttcccg tcaggcgaaa
     6841 atcttggaaa aatctccaaa ccttcatgcg aagtcaagcg tctggctaaa ccaccttggt
     6901 cagaaactaa atacaagtcg ccagcatagc taaacacaat tttatctcca taaatatctg
     6961 gaaaacgcat taaacgtgtt tcggcgtttt gagaaaataa actaaaactt aaaaatgaaa
     7021 taaaacaaaa aaccaaaaat ttcttcataa tctttaatat ttaaaatgtc tatacttttg
     7081 agttcgtgaa aataataaaa tttagggaaa ttagaaaatt aattttgaat ttaaaaagtg
     7141 ttgagttttt agtgttgagt ttttagtgtt tagtttttag tttttagttt ttagttggag
     7201 agagttgagt ttttagtttt aaatcacgaa agacacaaaa gtaagctcgt aaagtttata
     7261 aagaaaagcc aatcacgcaa gccactttta acacttcaac tacgctcagt gcatcgcttt
     7321 taactgatta cgcgtcagcc acttttaaca cttcaactat gttcagtgaa tcgcttttaa
     7381 cttttaactg aaattgtatt tgattctaat ttctgaccta atgcccaagt ttagaagttg
     7441 agtggctttg ccatcgcgta ttgctatttc taacaagccc aaagaattaa aaatgcaaac
     7501 aatttctcca ctatcaactt ctttataaga ttgttttatt tctgaaactg taatattatt
     7561 tgtatttatt aaaatttcaa attttttatc gccaacatag tctttaaaaa tttcgcgact
     7621 aatattagta attgcatttt ggtaagagtc tatataaata acttctccgt taatgaaatc
     7681 agtctctatt tggcaattta cactgagttt acgtttaatc ttatttatag gttctcctaa
     7741 ttctgtaata tctccgtctc taacaataaa tgcagctata tgagaaaata aatttaactc
     7801 aataaacgaa gctccttcaa atgcaaatcc tgtttctact aagacggcaa tttcgggttc
     7861 tttttcaaaa ataaaacctg tgcatccatt gttagagctt ataaaatact gagaatcgta
     7921 ttttgaaaca agaatataac cgtctctatc gggttcgctg tcaactccaa taatatgaat
     7981 gctgccctca ggaaaatttt tataaacaga ttttaaaaca aagccagcct gaaaaaccga
     8041 gaaatgctca atattatggc taatatcaat aaagctaaca tcgctacatt tcgaaattat
     8101 cgcagctttt acagcaccaa tgtagtaatc gctactcgac caatcagttg taaatgtaat
     8161 aattcttgcc aatttgttgt aaaaactatt taaaactaag taaaattaat ttgggtttaa
     8221 agttacaaat ttgttttggt ttagtaataa aaaattaaaa ttaattgaaa atctgttatt
     8281 ccataacgaa tatttctaaa aagttttaat tgcttgcaca aattcatcaa taccgtttac
     8341 agccgatttt tgaatatgtt taaaattatt aattccataa aaatcatcgc ttgggtcaat
     8401 tatgaaattt tgagattttt taggtaaata atttataagt cctgccgcgg ggtaaactgt
     8461 aagtcctgtt ccaattacta aaaaaatatc agcagttttt acaatatcaa ttgcgtgaga
     8521 aatatttggc acagcctcac caaaccaaac tatatgcggt ctaagttgag aacccaaagg
     8581 acataaatcg ccaagattta attcgcttcc ttgaatatca acaataatat tttcatcttt
     8641 agtgcttctg gctttgcgaa tttcgccatg taaatgtaag acatctgtag aacctgcacg
     8701 ttcatgcaaa tcatcaacat tttgagttat tatttttaca tcaaaatttt tatccaattc
     8761 ttttaaagtc aaatgtgctt tatttggttt ggaatcaaga actaccttac gtctaaagtt
     8821 gtaaaagtct aaaacaagtt gtggatttgc tttccatgct tctggtgatg ctatttcttc
     8881 aaatctatat ttgttccaca aaccgtcaga atctctaaaa gtaggaatac cgctttcttt
     8941 actaattcca gcaccagaaa gaacaactat ttttttcatt aaattaatct ttatatgatt
     9001 tgatatactc gtttaaagaa tttctagaat ttattgaagt aaaaaaactt gaaacaacag
     9061 aataattatc tctgtcgttg caatatttat atgcaaattt tgcaaaatct aacttgtcgt
     9121 tttctaaaga aaataaattt agcatagact taacttgttg tgctgaaata cattcatatt
     9181 gcggaattag attttttgct ttttcaagtt tttgagtaga actcatttct tttctaacat
     9241 cagatttaat tttttcgtaa tcgttatcac tcaatgtgca aggagcagga atgccaacag
     9301 cataaggttt ttcaggtttt ggcacttggt aaattgagtt aatttcatgt atataagatt
     9361 gtgttattag taattgtttg acatgcaagt agttgtctct gtcgtaaact ttttcgtaag
     9421 cagtttttag aaatacaaat ctcatttttt cgtttttaat caattctgct aattgcataa
     9481 cttgagcggt agagaagcaa tatgtataaa catattccat acatattttt gttttttctt
     9541 catcagtttc atatctggat agtgcatttt taaaagctaa aaactgttgt tcgtttaaac
     9601 aagattcgca acctttgcta cctgtatagc cttcagcttt tggataaaca atggaatttg
     9661 tagagcttgg ttctgttggt tttgtttctt ctttatcagt ttgcacagga gtttcgggtt
     9721 tttcaacaac ttgaggttct tgtggtttta cgggttctgt tttatcttta ggtttgtctt
     9781 tattttcgtt tttggcaatt tcgtcccttt gcttgtcttt tttcttttct tttaagctca
     9841 agtactttgt atagctctcc aaatccattg gtgcaattcc aagttgctca ttgttttttt
     9901 caatgaactc ccacaataat tgcacgtgta gatatttttc aaaagcattt gtaagcatta
     9961 aagcatttga tttatcagta atttttggat aagcttcttt tacatacaca agtctttgtc
    10021 cttcatcttg aattatcaaa gatgctttat acaattgatt tgatgaaata tgtttccatc
    10081 tcaagagctt aactgctttt attcgtcttt tttcttcagt ttttctgggt tttgaaatac
    10141 gttttgtgta tatcttatag ctaaatttcg acattggctt atgtgatttt acatcttgag
    10201 ccgaaatgcc tgaagctaaa attgcaaacg agagtaagaa taaaaatttt ttcatatcat
    10261 taatttttta cgaagataat taaatattta caaaaaaagt gcctaagttt agatttttag
    10321 gaaatacttt taaattttcc tttttagcac ttaaactacg tttagtgaat cacttttagc
    10381 tgactttatt gtgtcgccct tgcgggcgag ggagctgggg ttatctccac cgcaagataa
    10441 atcttacggt taatcacatg ttgcccttgc gggcaaggcg taattgtttg gcatgggtgc
    10501 tgcaatgctg aaaacaggag atttttggaa attatctttt tttagattaa gaccttcgta
    10561 tgggcaggta ttataaaaac tttgatagat aaattttgaa tgaatgaaac tcatcacttt
    10621 taacttgaca gtaaattgct gtcttgcaaa gatctcgtca aatgaatttg gtaatcgcat
    10681 ggaaactaga agaaacactt attaatttca aaaacatgaa actgtttggc acatattaga
    10741 ttttgcctta aaaattttgt attaggtaat aaaataataa ataaattata aacaacaaaa
    10801 ataaaattat ctcgttatac aaatattata atattaactt aaatttttaa aagtatagaa
    10861 gcaaaaaatg aaaaaacaat cgtgattaac ttgaattttt tttacgaaac taaggattgt
    10921 cctttaccag aagataattc agacattgta tttacagact tagctacaga attgatagag
    10981 aaaatacagt catttaagat tttttacaaa acattcatag agcctgagct aaaatctata
    11041 gcttatgaat ttttaagata ataaaggttt aaatttaata gatcattata ttattattta
    11101 aaaaaacagt agaattatga aaactattta ttttttaatg gcaatacttt ttgttttagt
    11161 ttttcaaata gtagatgccc aagaacaagt tgcatttaaa aaaacgaagt gggctttaat
    11221 attttaaatt actcatctat agacattcca tctgatcccc cacaaccaaa gtttgacata
    11281 agttttatta atggcattta ttttaagcgg cattataacg aaaatacatt acgactttct
    11341 gttgatcaat taacttgcaa tgtgaaaata cacggaaatg gttcttatga ggcagacact
    11401 ctaataacaa tgggaaattg gagagaacaa atgttttcta tgggatttga acaaaattat
    11461 tttgaaaaaa aacagtttca tctttactgg ggagttgact taaatctgta ttatggaatt
    11521 tatagtgggc aatcacaatc tttaagtggg ttgtctaatt tttctacagt aaaacaaaat
    11581 aagggaatag gtcttactcc gttagctggc atacgtttta caatttcgaa tttccttaga
    11641 atatctttag aaacatctgg tagtagtctg ttttttacag aatttgagaa taaaaaatgg
    11701 acactaccta tggtaaaaaa tgagcaaaat caagcaatta cttttgctct taaatataat
    11761 gtaggctgta aaataagtta tttattttag aaattatagt ctaataatat agtctatcgc
    11821 tcacttggat tgcaacctaa gtccgcagta cgagttcagc taggtaaatc cgagcgagta
    11881 aactgctaaa tttttatttg ttttaattat tgatttgttt tatatttgaa ga
//
LOCUS       DAOPNX010000079        11929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_6187_length_11929_cov_6.361546, whole genome shotgun sequence.
ACCESSION   DAOPNX010000079 DAOPNX010000000
VERSION     DAOPNX010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11929
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6187_length_11929_cov_6.361546"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2373
                     /locus_tag="PKN53_08650"
     CDS             <1..2373
                     /locus_tag="PKN53_08650"
                     /inference="COORDINATES: protein motif:HMM:NF019295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD89056.1"
                     /translation="DTPDDSPLLILSEDFESGMPSDWDLIDADGDGNNWYLDTYTGHN
                     SNNCVSSESYDYNNSEALSPDNYLISPLLNNASKMEYWVFAVDNTPYEHYAVMASTTG
                     NSISDFTNVFEETMTNPNHWYKRSINLPPETKYIAFRHYNCTNQKGLKIDDISIYGNI
                     IPTTAPHTYTITRNGEEIASGLTEKTYTDLDLDFNTYNYCVKAVYGYCTSETACTNVI
                     IEDSLCLPISDLSVIINGNDVNLTWEDPSAEETYTYTITRNEVEIANGLTETTYTDLD
                     LDFGTYNYCVKAVSEDCTPQAICESIILEDQSICLPVNNLNATVNENNTVSLNWLIPQ
                     QTDWPSNCSEDIAFRIYKGTTQIGYSDANDLTSWTTNVLSNGTYELKVVILYYDNNSN
                     LLCTAEANTTVNITNSFVCLPVNNLSAVVNNNNTVSLNWTIPEELPISCDGNIAFKFY
                     NGNINIGSSNNDNLTSWTTPELSIGTHFLTVSIYYLDNTNAEICYAETSITVTIESSF
                     DCQAVNNLSATVNSDNTVDLTWDIPAETEWPNACAGNLAFHFYNGSTKLGESDEDNLT
                     LWTTGILPNGTHTLKVVIYYYDNNTNLICSAEASITVIVSGSFACPSLNELSAIVNEN
                     NTVNLSWTLPTDLPTNCNGNLDFKFYDGTTQIGYDISDDLTSWTTPVLSAGTHTLAVV
                     VYYYDDEDIEICYAEASVTVTTDIEEIKVSLFSAEIFPNPAKDLLNIKCDSEIISYEL
                     YDALGRLLINKKEVANTESIVNVSSLKHGMYMLRLNTEKGSGTFKLIIDN"
     gene            2618..3325
                     /locus_tag="PKN53_08655"
     CDS             2618..3325
                     /locus_tag="PKN53_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOD89057.1"
                     /translation="MSRYFEEPNCKDCNLVFSTVFKKLTVDQLDYLTKNKVCSQIKKG
                     KTLYKEGSRITGVYCINDGILKVYKTGCEGKEQIVKFAKRGDIIGYRSVINGEPACTS
                     VKAIEDSVVCYINTQELYGLLKGNSELGIELLQIACAELDEANNYLTDIAQKSVRERL
                     AEILLKLQDDFGTDKEGIINITLTREELANIIGTATESVIRLLSEFRTEGLVDLQGRK
                     IKILNIQKLKKISNVFK"
     gene            3328..4425
                     /gene="mqnC"
                     /locus_tag="PKN53_08660"
     CDS             3328..4425
                     /gene="mqnC"
                     /locus_tag="PKN53_08660"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HOD89058.1"
                     /translation="MPDNQKTNSILEKAYNLEFLSFNECIFLYQHASLSQLLFVANQI
                     RQKIHPNNIVSYIIDRNINLTNVCVSNCLFCNFCRNKNSEQAYVLSIEEYIEKIEHLK
                     SLGGNQILLQGGMNPALDLEYYETLFKKLKKYYPDLKLHALGPPEIAFLAKQEGISTK
                     RCLQILINAGLDSLPGGGAEILSDRVRKLVSPAKVCSEQWLNIMHEAHQLGLTTSATM
                     MYGHLETIEERIEHFLKLRDIQKIKPENSKGFISFTSWSLAGKNTRLLKKYPDIKTVN
                     STEFLKMLALSRIILINIPNIQVSWLTRGKEIAKLALHGGANDMSSIMIEENVVSQAG
                     KNFKLGVKEMEETILEAGFIPQLRNQEYEYL"
     gene            complement(4420..5277)
                     /locus_tag="PKN53_08665"
     CDS             complement(4420..5277)
                     /locus_tag="PKN53_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89059.1"
                     /translation="MKIIKLKHSEIDFEKYDKTLLECKWARVYAMSWYLDIVSPNWEL
                     LCDEDYNIVMPLPKKKKFGIPYLIQPMFCQQLGVFAKTEIDKNQIENFYKKLGYYFVI
                     IHGNSFDSMIFSEDKLKPNYFISSKNFEEQQKLFSKNTIRNLKKADSFNHKIITIEIQ
                     EYFNFLTKEASNIYNEKLQNALRKIVKKSLDLGYGKLLATTVENEVSSVVFILDFNNR
                     IYYLAPASSQKGKEARSMMFIINDLIKTSIEQGKIFDFEGSSLEGVERFYKSFGASCE
                     YYGIFKKIL"
     gene            complement(5297..6415)
                     /locus_tag="PKN53_08670"
     CDS             complement(5297..6415)
                     /locus_tag="PKN53_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOD89060.1"
                     /translation="MRKNKDDIKLVFKTLNERKKELDCIYRIDELLKDFDSDLHNILT
                     GICEFAPIGWRYSEICKVKIIVNGFAVESTGFKETELKLSANLEVDGEKVGEFIICYI
                     KSVKSEKGVFLPEETRLFKTIVEKINQYLSFRKLKEFYANTIENKNKLYGKDISFTDY
                     LKSLKLSNEDIEIITKVEVDFKKGETVCKQGTFASFIMLIKSGMVKSFIENSHYKSHI
                     FKFTGAFNIIGLSTLYGDSYYHFSCKALVPSKFYLVERSEFDKIVRKNPDFAIEIMKI
                     YSKSLQTVYDKLGSIANKQSIGKVCVSLLYLAENVFKSNIIDTSVTRKDIAEFSGLAT
                     ENLVRILSELKKDNIIAINNRVIEILNFETLKMLSNIG"
     gene            6610..7950
                     /gene="gdhA"
                     /locus_tag="PKN53_08675"
     CDS             6610..7950
                     /gene="gdhA"
                     /locus_tag="PKN53_08675"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOD89061.1"
                     /translation="MNVEKFMSDLEQRHPGEKEYLQAVHEVVESVKEVYEKHPEFAAA
                     GILERIVEPDRVFTFRVAWVDDKGKVQTNIGYRVQFNGAIGPYKGGIRFHKAVNLSML
                     KFLGFEQTFKNALTTLPMGGGKGGSDFDPVGKSNGEIMRFCQAFMWELWRCLGKDTDV
                     PAGDVGVGAREVGFMYGMYTKLAREYNVGVFTGKGINWGGSKIRPEATGYGALYFVDH
                     MLAAAGKDIKGKTVVCSGFGNVAWGATQKAKELGAKVIGISGPDGVCHIPDGMTDEMN
                     AYMLELRASNNNVVAPMATKFKGVTFTPGKKAWSIKCDIALPCAFQNELDGNDAKELL
                     KNGVMLVAEVSNMGCTPEAIHEFQKAKIMFAPGKAVNAGGVATSGLEMTQNAMHLSWS
                     EKEVDDRLHEIMQSIHEACVTHGTEKDGYINYVKGANIAGFMKVARAMVEQGVV"
     gene            complement(8021..8608)
                     /locus_tag="PKN53_08680"
     CDS             complement(8021..8608)
                     /locus_tag="PKN53_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOD89062.1"
                     /translation="MSPKTSGQFKEIRADKKKLIIDTALNLFANRGYHASSISMIAKE
                     AKISKGLMYNYFDSKEALLREIFEDFIRIIMDLMNPNHDDEITTEEMRDFLSSYFQLV
                     EQNHLYWKLYFQLSVKSEIFDLLVNDSYSEQMEKTQRLINRYFSERFKEPDIEISLFF
                     SILKGFALDYLHSYKTYPQSKIEGFKKKMFEMFLR"
     gene            complement(8674..9369)
                     /locus_tag="PKN53_08685"
     CDS             complement(8674..9369)
                     /locus_tag="PKN53_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOD89063.1"
                     /translation="MKLLKTYIEDFWFLLYPHTCEACGVALNKNEGIICFKCLYDLPR
                     TDYCEFIENPITELFAGRLKLERGTALFTFHKGSRFRKLLHSLKYKNKPEIGIVLGRE
                     LGAQMLKSNNFMDIDFLIPVPLHEKRKKQRGYNQSEMIAEGISQVTKIPFVVDNLVRA
                     KETVTQTKMTKEERWANVSGKFLLNNPSELEGKHVMLVDDVVTTGSTIEACGEVLLTV
                     ENLKLSIAVLAKA"
     gene            9434..10417
                     /locus_tag="PKN53_08690"
     CDS             9434..10417
                     /locus_tag="PKN53_08690"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HOD89064.1"
                     /translation="MIFRTIIEIPKFDFDISHSDACVFSGSCFAENIGEKLFDNKFNV
                     SVNPTGIHYNPISLAKSVNMAISSKQITENEVFNANSLFNHFNFHSQFSDIDKTQAID
                     NFNKAIQTFNEALTKSKYFFITFGTSFVYKLKENNEIVSNCHKLPEKVFVRDFLSSHE
                     IVETWINLIKKIKEINPEIKLIFTVSPIRHLKDGAIQNQQSKANLIISVKSIIENTDC
                     CFYFPAYEIINDELRDYRFYAEDMIHPSKTAIDYIYQKFSNSFFSIKTQNINKQIEKI
                     ISAYNHKVFNPNSEAHKKFCLNILKDIDDLKKQGVHLDFSNEIKYFKSFLK"
     gene            10535..11200
                     /locus_tag="PKN53_08695"
     CDS             10535..11200
                     /locus_tag="PKN53_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89065.1"
                     /translation="MKRILLISLAVIFSSTIFAQKIEVREVTGKFRNGNQNALSTFVY
                     HSDIKTVRKEFESLLKSYKGKVSTKKGETFGDDLLIASVSNNTLDVYAYCVDTKDGAI
                     EVVAGFDLGGAYLSSSMHQEQYARVAQIMREFALKITEKAYADFLKGEAKDLKSATKA
                     HEKMVKEKSNLEKQNQDYKKRIDQNEKAIEKLGKQIEEDATRLKEMTTEYEKLKKAES
                     KIK"
BASE COUNT         4335 a         1846 c         1772 g         3976 t
ORIGIN      
        1 gatactcctg atgattcgcc tttgcttata ttaagcgaag attttgaaag tggaatgcct
       61 tcggactggg atttaattga tgcagacggc gatggaaata attggtactt agacacatat
      121 acaggacata atagtaataa ctgtgtaagt tctgaatctt atgattacaa taattcagaa
      181 gctctaagcc ccgataacta tttaatttca ccattattaa acaatgcatc taaaatggaa
      241 tattgggttt ttgcagttga caacacacca tacgaacatt atgcggttat ggcttcaaca
      301 acgggaaata gcattagtga tttcacaaac gtttttgaag aaacaatgac aaatcctaac
      361 cattggtata aaagaagcat taatttacca cctgaaacca aatacattgc tttccgtcat
      421 tataattgca ccaatcaaaa aggtttaaag attgacgaca taagtatata tggaaatatt
      481 attcctacta cagcacccca cacttatact attactagaa atggtgaaga aattgcaagt
      541 ggacttacag aaaaaactta cacagactta gacctagatt tcaacacata taattattgt
      601 gtaaaagctg tttatggcta ttgtacttca gaaacagctt gtacaaatgt tataatagaa
      661 gatagtcttt gcctacccat aagcgatctt agcgtaataa taaatggcaa tgatgtaaat
      721 ttaacatggg aggatcctag tgcagaagaa acctatactt acaccattac tagaaacgaa
      781 gttgaaattg caaatggact tacagagaca acatatacgg acttagacct agatttcggc
      841 acatataatt attgtgtaaa agctgtttcc gaagactgta ctccccaagc tatttgcgaa
      901 agtattatat tagaagatca aagtatttgt ttgcctgtaa acaaccttaa tgcaacagta
      961 aatgaaaaca atactgtaag tttaaactgg cttataccac aacaaacaga ttggcccagc
     1021 aattgctctg aggacattgc ctttagaatt tataaaggaa ctactcaaat tggttatagc
     1081 gatgcaaacg atttaacttc atggacaact aatgtgcttt caaatggaac atacgaacta
     1141 aaagttgtta tactttatta tgataacaat agcaatcttc tttgtactgc cgaggctaat
     1201 acaactgtaa atataacaaa tagtttcgtt tgcttacctg tcaataacct tagtgccgta
     1261 gtaaacaaca ataatactgt aagtttaaac tggacaattc cagaagaatt acctattagt
     1321 tgtgatggaa acattgcttt taaattttat aatggtaata ttaacatagg ttctagtaat
     1381 aacgacaatc taacttcttg gacaacaccc gaactctcaa taggcactca ttttttaact
     1441 gttagtatat attatttaga taacacaaat gctgaaattt gttatgccga aacaagcata
     1501 acagtaacaa ttgaaagcag ctttgattgc caagctgtta ataaccttag tgcaacggta
     1561 aactcagaca atacagtaga tttaacttgg gatattccag cagaaacaga atggcccaat
     1621 gcttgcgctg gaaatcttgc cttccatttt tataatggat ctactaaatt aggtgaaagt
     1681 gatgaagaca atttaacttt atggacaact ggcatacttc caaatggaac acatacacta
     1741 aaagttgtta tatattatta tgataacaat accaatctta tttgttctgc cgaagcaagt
     1801 ataacagtaa tagttagtgg tagctttgct tgtccttctc ttaatgaact tagtgcaata
     1861 gtaaacgaaa ataatactgt aaacttaagt tggacactac caacagattt accaaccaat
     1921 tgcaatggta atcttgactt taaattttat gatggaacta ctcaaatagg ttatgatatt
     1981 agcgatgatt taacttcttg gacaactcct gtactgtcgg ctggcacaca cactttagct
     2041 gttgttgtat attattatga tgacgaagat attgaaattt gttatgcaga agcaagtgta
     2101 actgtaacaa ctgatattga agaaataaaa gtatcgctct tttctgcaga aatatttcca
     2161 aatcctgcaa aagatttact aaacatcaaa tgcgatagcg aaattataag ctacgaactt
     2221 tacgatgcac tcggcagatt attgataaac aaaaaagaag ttgctaatac tgaaagcata
     2281 gtaaatgtat catcgcttaa acatgggatg tatatgctta gacttaatac tgaaaaggga
     2341 agcggaactt ttaaattgat aattgataat tgaataattg aataattgaa taatttagct
     2401 tcgctacatt ttctgtcgtc tctcggcata gttcaagcaa gcttgactct gcactcgctc
     2461 cttagaaaat tgataattaa gaatgagtta aaagtttata aagtaaaaag tttgtaaagg
     2521 caagccgact ttcgactttt aacttttaac ttttaactgt attatcgatt tgtgatttta
     2581 atcatatttt tataattttg cacaaaattt tgacaatatg agcagatatt ttgaagaacc
     2641 aaattgcaaa gattgtaatc ttgttttttc tacagttttt aaaaagctaa ctgttgacca
     2701 actagactat ttaacaaaaa acaaagtgtg ttctcaaatt aaaaaaggta aaacacttta
     2761 caaagaaggt agccgcataa caggagtata ttgcatcaat gacggaattt taaaagttta
     2821 taaaactggt tgcgaaggaa aagaacagat tgtaaaattt gctaaaagag gcgacataat
     2881 tggttatcgc tcggttataa atggagaacc tgcttgcaca agcgtaaaag ctattgaaga
     2941 ttctgttgtt tgctatataa atactcaaga actttatggg ttactaaaag gaaatagcga
     3001 actaggaatt gaacttttac aaattgcatg tgcagaactt gacgaagcaa ataattatct
     3061 cactgatatt gctcaaaaat ctgtaagaga aagacttgct gaaatattac taaaacttca
     3121 agacgatttt ggtactgaca aagaaggaat aataaacatt actttaacac gcgaagagct
     3181 tgcgaatatt attggaactg ctacagaatc tgttattaga ttgttgtctg aatttagaac
     3241 cgaaggactt gttgacttac aaggaagaaa aattaaaatc ctaaacattc aaaaactaaa
     3301 aaaaattagt aatgttttta aataaaaatg cctgacaacc aaaaaactaa ctcaatatta
     3361 gaaaaagctt acaatcttga atttttatca tttaatgaat gtatattttt gtatcaacat
     3421 gcaagtttaa gtcagttgct atttgttgct aaccaaattc gtcaaaaaat tcacccaaat
     3481 aatattgtaa gttatataat tgatagaaat atcaatctca caaatgtttg tgtttcaaat
     3541 tgtttgtttt gtaatttttg tagaaataaa aattctgagc aagcatatgt tttaagcatt
     3601 gaagaatata ttgaaaaaat tgaacactta aaatctttgg gaggaaatca aattttattg
     3661 caaggaggaa tgaatcccgc acttgacttg gaatattacg aaactttatt taaaaaatta
     3721 aaaaaatatt atccagattt aaaacttcat gctttaggtc cacctgagat tgcttttctt
     3781 gcaaaacaag aaggaataag cacaaaacga tgcttgcaaa ttcttataaa tgcaggttta
     3841 gatagcttgc caggtggagg tgcagaaatt ttgtcggata gagttcgcaa attggtttcg
     3901 cctgcaaaag tttgctccga acaatggcta aatattatgc acgaagctca tcaactaggc
     3961 ttaacaactt ctgctacaat gatgtacgga catttagaaa ctatagaaga aagaattgaa
     4021 cattttttaa aacttagaga tattcaaaaa ataaaacccg aaaattcaaa aggatttatt
     4081 tcatttacct cttggtctct tgctggcaaa aatacaagat tgctaaaaaa atatcctgat
     4141 attaaaactg ttaactctac agaatttcta aaaatgttgg ctttgagcag aataatattg
     4201 ataaatattc ccaatattca agtttcgtgg ctaactagag gtaaagaaat tgcaaaacta
     4261 gccctacacg gaggagcaaa cgatatgagc agtataatga tagaagaaaa cgtggtttcc
     4321 caagctggca aaaattttaa actcggtgtt aaagaaatgg aagaaacaat tttagaagca
     4381 ggttttattc cacaattaag aaatcaagaa tatgaatatt tataaaattt tcttgaaaat
     4441 accgtaatat tcgcaactag caccaaaact tttgtaaaat ctttcaactc cctctagaga
     4501 agaaccttca aaatcaaata tttttccttg ttctatagaa gtttttatta aatcattgat
     4561 tataaacatc atagaccttg cttcttttcc tttttgagac gatgctggtg caagatagta
     4621 tattctatta ttaaaatcaa gaataaaaac aactgagcta acttcatttt caactgttgt
     4681 agcaagcaat tttccataac ctaagtccaa agacttttta acaatttttc tcaatgcgtt
     4741 ttgcaatttt tcattataaa tattgctagc ttcttttgtt agaaaattaa aatattcttg
     4801 aatttctatt gttataattt tatgattaaa actatcagct tttttcaaat ttctaattgt
     4861 attttttgaa aataattttt gttgctcttc aaaattttta gaactaataa aataatttgg
     4921 ctttagtttg tcttcgctaa aaatcattga atcaaaacta tttccatgaa ttattacaaa
     4981 ataataacca agctttttat aaaaattttc aatttgattt ttatcaattt cagtttttgc
     5041 aaaaacacct aattgctgac aaaacattgg ttgaattaaa taaggaatgc caaatttttt
     5101 cttttttgga agtggcatta caatattata atcttcatcg cataataact cccaatttgg
     5161 cgacacaata tctaaatacc aagacattgc ataaacccta gcccacttac attctagtaa
     5221 agttttatca tacttttcaa aatctatttc actatgtttg agctttatta ttttcatttt
     5281 gcctttgatt aaaactttag ccaatattac tcaacatttt taaagtttca aaatttaata
     5341 tttctattac tctattatta attgcaataa tattatcttt tttcaattct gataaaattc
     5401 tcactaaatt ttcggttgcc aaacccgaaa actctgcaat atctttgcgt gtaacacttg
     5461 tgtcaattat atttgattta aaaacatttt ctgccaaata caaaagcgag acacatactt
     5521 tgccaataga ctgtttattt gcaatacttc caagcttatc gtaaacagtt tgcaaagact
     5581 ttgaatagat tttcatgatt tcaattgcaa aatcaggatt tttcctaact attttatcaa
     5641 actcacttct ttcgaccaaa taaaacttag aaggcactag cgccttgcac gaaaaatgat
     5701 aataagaatc gccatacaaa gttgataagc caattatatt aaaagcacca gtaaatttaa
     5761 aaatatgact tttatagtga gagttttcaa taaaagattt taccattccc gactttatta
     5821 acattataaa cgaagcaaaa gtgccttgtt tacaaacagt ttctcctttt ttaaaatcaa
     5881 cttctacttt tgtaattatt tcaatatctt cgttactaag tttaagactt tttagataat
     5941 cagtaaaact tatgtctttg ccataaagct tattcttatt ttcaatggta tttgcataaa
     6001 attcttttaa ctttctaaaa ctcagatatt gattaatttt ttcaactata gttttaaaaa
     6061 gtcttgtttc ttctggcaaa aacactcctt tttcggattt aacagatttt atatagcaaa
     6121 taataaattc gccaactttt tctccatcaa cttccaaatt tgcactaagt ttaagttcag
     6181 tttctttaaa accagtactt tcaacagcaa aaccatttac aataatttta actttacaaa
     6241 tttcagaata tcgccaacct attggcgcaa attcgcaaat gcctgttaaa atattatgca
     6301 aatctgaatc aaaatctttt aaaagctcat ctattctata aatacaatca agttcttttt
     6361 ttctctcgtt cagagttttg aatactaatt ttatatcgtc tttattcttg cgcattatat
     6421 tttaaaattt atgcaaaatt aataaaaatc atatacctaa caaaaatcat tgataaatat
     6481 caataaaaat tatcaaaaca aatcaagtat aaaattagct atattccaaa ctttatttta
     6541 actttacatc gttaattaaa aaaaataaac catagtggtt ttatagtaaa tatttaaaaa
     6601 tttataaaaa tgaacgtaga aaaattcatg agtgacctgg aacaaagaca tccaggggaa
     6661 aaagagtatt tgcaagctgt gcatgaagta gtagaatcag ttaaagaagt ttatgaaaaa
     6721 catcctgagt ttgctgctgc cggcatttta gaacgcattg ttgaaccaga cagagttttt
     6781 actttccgcg ttgcatgggt tgacgataaa ggaaaagttc aaacaaacat aggttacaga
     6841 gttcaattta acggtgcaat tggtccatac aaaggtggaa tcagatttca caaagctgta
     6901 aatctttcga tgttgaaatt tttaggattc gagcaaactt ttaaaaatgc tttaacaaca
     6961 cttcctatgg gtggtggtaa aggtggttct gattttgacc ctgttggaaa atcaaatggc
     7021 gaaatcatgc gtttttgcca agcttttatg tgggaattat ggcgttgcct aggtaaagat
     7081 actgacgttc ctgctggaga cgttggcgtt ggagcacgtg aagttggttt catgtatggt
     7141 atgtacacaa aacttgctcg cgaatacaac gttggagttt tcactggtaa aggtattaac
     7201 tggggtggtt caaaaattcg tccagaagca actggctacg gagctttata ttttgttgac
     7261 cacatgttag cagcagcagg taaagacata aaaggtaaaa ctgttgtttg ctcaggtttt
     7321 ggaaacgttg cttggggtgc tactcaaaaa gcaaaagaac ttggtgctaa agttataggt
     7381 atttctggac ctgatggcgt ttgccacatt ccagatggta tgacagacga aatgaacgca
     7441 tatatgttag aactaagagc ttctaacaac aacgttgttg caccaatggc tactaaattt
     7501 aaaggcgtta ctttcacacc tggtaaaaaa gcttggagca taaaatgtga tatagctctt
     7561 ccatgtgcat tccaaaatga gttagacggt aatgatgcaa aagaattact caaaaacgga
     7621 gttatgttag ttgctgaagt ttctaacatg ggctgtacac ctgaagcaat tcacgaattc
     7681 caaaaagcaa aaattatgtt tgctcctgga aaagctgtaa acgctggtgg agttgcaact
     7741 tcaggtcttg aaatgaccca aaacgctatg cacttatcat ggagcgaaaa agaagttgac
     7801 gacagacttc acgaaattat gcaaagcata cacgaagcat gtgttacaca cggaactgaa
     7861 aaagatggtt atataaacta tgtgaaaggt gctaacatcg ctggtttcat gaaagttgct
     7921 cgcgcaatgg ttgaacaagg agttgtttag actcaatata atgcttaatt tttaatttat
     7981 gaagttggct gagcttaggc aaagccaact tttttttatt ttatcgcaaa aacatttcaa
     8041 acattttttt cttaaaacct tctatttttg attgaggata agttttgtat gaatggagat
     8101 aatctaaagc aaaacctttt aatattgaaa aaaacaaact aatttcaata tcaggttctt
     8161 taaaacgctc tgaaaaatac ctatttatta atctttgcgt tttttccatc tgctcactgt
     8221 aagaatcatt tacaagtaaa tcaaaaattt cgcttttaac agaaagttga aaatataact
     8281 tccaataaag atgattttgt tcgactaatt gaaaataaga agataaaaaa tctcgcattt
     8341 cttcagtagt aatttcatca tcgtggttag gattcattaa atccataatt attctgataa
     8401 aatcttcgaa aatttcgcgt aataaagctt ctttactatc aaaataatta tacatcaaac
     8461 cttttgaaat tttagcttcc ttagcaatca tgcttataga acttgcatga taacctctat
     8521 tagcaaaaag atttaatgct gtatcaataa taagcttttt tttatctgct cgtatttctt
     8581 taaattgacc cgatgttttt ggcgacattt ttttcaattg aacggttgtt caaagataaa
     8641 ctattttaat ttattacaaa aattaatttt taattatgct tttgccaaaa ctgctatact
     8701 taactttaaa ttttctacag tcaacaatac ttctccacaa gcttcaatag tagaacctgt
     8761 agtaactaca tcatctacaa gcatcacgtg tttaccttct aactcagaag gattgtttaa
     8821 taaaaatttc ccagaaacat ttgcccatct ttcttctttt gtcatttttg tttgtgtaac
     8881 agtttcttta gctctaacaa gattatccac aacaaacgga atttttgtaa cttgtgaaat
     8941 accttctgca atcatttcac tttgattgta acctcgctgt tttttacgtt tttcatgcaa
     9001 aggaacagga attaaaaaat ctatatccat aaaattattg gattttagca tttgtgctcc
     9061 aagctctctt cctaaaacaa ttcctatttc tggtttattt ttatatttca aactatgtaa
     9121 taattttcgg aatctacttc ctttatgaaa tgtaaataat gctgtgcctc tttcaagttt
     9181 taatcttcct gcaaataatt cagttattgg attttcaata aactcacaat aatctgttcg
     9241 tggcaaatcg taaagacatt taaaacaaat tattccttca ttcttattta aagccacacc
     9301 acaagcctcg catgtatgag gatataataa aaaccaaaaa tcttctatat atgtttttaa
     9361 aagtttcaaa aagtttttgt attttagatt tattgtaaat ataggtttat ttttgtaata
     9421 aaaaattaaa aaaatgattt ttagaactat aatagaaatt ccaaaatttg attttgatat
     9481 ttcacattct gatgcttgcg tgttttcagg ctcgtgcttt gccgaaaata ttggtgaaaa
     9541 attatttgat aataaattta atgtttcggt aaatccaaca ggaatacatt ataatccaat
     9601 ttctctcgcc aagtctgtta acatggctat ttcctcaaaa caaattacag aaaatgaagt
     9661 atttaatgca aattctttgt tcaatcattt taattttcat tctcagtttt ccgacataga
     9721 caaaactcaa gccatagata attttaataa ggcaattcaa acttttaatg aagctcttac
     9781 taagtcaaaa tactttttca taacattcgg aacttcattt gtttacaaac ttaaagaaaa
     9841 taatgagata gtttccaatt gtcataaatt gcctgaaaaa gtatttgtta gagatttttt
     9901 atcatcacat gaaattgttg aaacttggat aaatttaatt aaaaaaataa aagaaattaa
     9961 tcccgaaatt aaactgattt ttaccgtaag tcctataaga cacctaaaag atggagctat
    10021 acaaaatcaa caaagcaaag ctaatttaat tataagtgtc aaaagcatta ttgaaaatac
    10081 agattgttgc ttttattttc ctgcttacga aataataaat gatgaattaa gagattatag
    10141 attttatgca gaagatatga ttcacccatc aaaaactgca atagattaca tttatcaaaa
    10201 gttttcaaat tcattttttt cgataaaaac acaaaatata aataaacaaa ttgaaaaaat
    10261 tatttcagcc tataatcata aagtttttaa cccaaattct gaagcacata aaaaattttg
    10321 tttaaatatt ttgaaagata ttgatgactt aaaaaaacag ggtgttcatc ttgatttttc
    10381 caatgaaata aaatatttta agtcatttct gaaataacaa ctacttttcg aaaacatttc
    10441 gaatagattt ttgttaataa atattaattg ataatacaag aatttgtatt atttttgtta
    10501 aaattaaaaa ttactaataa aaaataaaaa aattatgaaa agaattttat taataagttt
    10561 agctgtaata tttagcagca caatttttgc tcaaaagatt gaggtaagag aagtaacagg
    10621 aaaattccgc aatggaaatc aaaatgcatt atctactttt gtatatcaca gtgatataaa
    10681 aactgtaaga aaagaatttg aaagtttgct aaaatcttac aaaggtaaag tttcgactaa
    10741 aaaaggtgaa acatttggtg acgatttatt aatagcttcg gtaagcaaca acactttaga
    10801 tgtttacgct tattgtgttg acacaaaaga tggtgctatt gaagtagttg ctggttttga
    10861 cttaggtggt gcttatttat cttcttctat gcaccaagag caatatgcaa gagtagctca
    10921 aattatgaga gagtttgctt taaaaattac tgaaaaagct tatgcagatt tccttaaagg
    10981 cgaagctaaa gatttaaaat ctgcaactaa agctcacgaa aaaatggtaa aagaaaaatc
    11041 aaatcttgaa aaacaaaatc aagattacaa gaagagaatt gatcaaaatg aaaaagctat
    11101 agaaaagtta ggcaaacaaa ttgaagaaga tgctacaaga ctaaaagaaa tgacaaccga
    11161 gtatgaaaaa cttaaaaaag cagaatcgaa aattaaataa atattaaaca aaacaaatta
    11221 aatcagttaa aagttaaaag ttaaaagtcg aaagagtttt tagtgttgag tttttagtgt
    11281 ttagtctatt gctcgaacgg atttaacttt acgaacttta aaaactttat gaacttttaa
    11341 ctggattaat tgtgttgccc ttgcgggcaa agcctttgag ggtctaacta ccgcaggctg
    11401 ccgcctacgg ttaatcacat gttgtcctac ggacaaactt tcgacggtct tgttgtgttg
    11461 tcctacggac aactaacatc tgctatcttc aacacccatg acgctcgctc caacacccat
    11521 tatgctcgct cggatttgta atccgagtgt aacattacca agcactcgga ttgcaaatcc
    11581 gagcgagcgg agatagtaat aaaaatttcc gtccttacga actttaaaaa ctttatgaac
    11641 tttagaaact ttaaaaactt tatgaacttt aaaaacttta tgaactttta acacattacc
    11701 aagcactcgg attgcaaatc cgagcgagcg gagatagtaa taaaaatttc cgtccttacg
    11761 aactttaaaa actttatgaa ctttaaaaac tttaaaaact ttatgaactt tagaaacttt
    11821 taactgtgtt acaccgtcat tatttctttt tctttgttat cgataacgat atcaactttt
    11881 ttaatataat cttcgtgagt tttatgcact tcttgctctg ccattttag
//
LOCUS       DAOPNX010000080        11815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_6256_length_11815_cov_6.525425, whole genome shotgun sequence.
ACCESSION   DAOPNX010000080 DAOPNX010000000
VERSION     DAOPNX010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11815
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6256_length_11815_cov_6.525425"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..3087)
                     /locus_tag="PKN53_08700"
     CDS             complement(19..3087)
                     /locus_tag="PKN53_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOD89066.1"
                     /translation="MKNISAILVLIFVVSNAFCQNFETGIPGFKNFSPRNYGQESQNF
                     SVVQNKEGIMYFGNANGIMEFDNSSWRLVKVPGKPMLTINSNNEVYYGGYNQIGKIVY
                     ENGHLLTKLLNLGEHDKFGQVTQIIAIDNDLYVVSSKQLFYFHRDSLQSVKTFQSNVK
                     IFKFNEKILCNIQEKGLSYCQNGDFVKFEDSELFENMTICDIIALNSKEFLVRDQKSE
                     SFFKYSDGTITEFKTEADDFIQANTYSVGLALDNKRMVFGTEHGGIVCVDEKGHYVFS
                     MSRDNSLRDNQITSMYVANDGNLWLTTFSGISLIETKSNISFFGSYYGLNGVILSVIR
                     FESDLYVATTNGVYLYNCQSVYDKNRNSFDYKAKFKKIDEIRAMCWKLVEIQGNLYAV
                     TNDGIYLINGSKAKLQLSGNFMSISKMSFFPDNILIGSKDGLLIASVEDSKIDTLGYV
                     KNYSYSTRTICEDFFGNFWLGTSEDGLFQLSFFSGLNLNPDIISFAEKTGLPEDYDWI
                     DVFPSFQGVLFATNKGVYRYSYQGNYFFEDTLFNKNYAKTNSYLYPLVEDKNKNIWYS
                     NITDGRYERETGLFEFQDSNSSYTAVAGPYSQLKEFVIESIYPEDDGNVWLGSTEALI
                     KIDNEKTKFYQKKINVLIRKITIHGDSLIYVPFNCSGEEKYEFKFADKTIRFDFTALN
                     YNSFGQAKFQIKLVGFDNDWSDWQSANFKEYTSLFEGDYEFQIRAKDEYGNISDVTSL
                     FFTIRAPVYRTWYAFLFYVLILFTAVFLILKLYALRHAHERYSLEKLVEDRTNELAYQ
                     KEQTEQLVKKLLPKTTADELRETGKAQSQKYEMVTVLFADIQGFTKIAAQAKPEELIK
                     HLNRLFTSFDKIIAKYNIEKIKTIGDAYMCAGGMPNKDSTNPVEVVLAALEMQRKITK
                     INAESDVKLEIRIGVHTGPVVAGVVGSQKLEYDIWGDTVNIASRMESYGVVNKVNVSE
                     STYKWIKDFFVCQYRGKIDVKYKGEMEMYFVHEIKKSLSFLLSNYYLLNA"
     gene            3167..4369
                     /gene="hydF"
                     /locus_tag="PKN53_08705"
     CDS             3167..4369
                     /gene="hydF"
                     /locus_tag="PKN53_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOD89067.1"
                     /translation="MALGKENKIHIGIYGRRNVGKSALINFLSGQNISIVADLPGTTT
                     DPVKRSFEITGFGPAIFIDTAGIDDAGEIGDLRTKKTLDTTDKVDLAILVFDSNTFGK
                     YEESLINNFNNAKLPYILLHSKSDIQSLNTDLEQKLKNNFTKNIIDFSIKDLTKRDEF
                     FELIKSTIPDLSLKTDTLLGDIIKKGDLILLVSTIDAGTPAGRLILPQVQTIRDVLDN
                     DAISIILKQDEIKDFLEKSKVKPNLVITDSQIFDTVSKLIDKSILLTSFSIVLARQKG
                     DFQAYIEGTPHIENLKDGDNILILESCSHHVSCDDIGRKKIPTLLRNYTKKELNFTVV
                     SGLDNIPEAIQNYALVIQCGGCVLTKKQVQNRLKPAVDAKIPVTNYGMLIAYVHGIFE
                     RAVQVFKK"
     gene            4422..4505
                     /locus_tag="PKN53_08710"
     tRNA            4422..4505
                     /locus_tag="PKN53_08710"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4456..4458,aa:Leu,seq:caa)
     gene            4546..4986
                     /locus_tag="PKN53_08715"
     CDS             4546..4986
                     /locus_tag="PKN53_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOD89068.1"
                     /translation="MNKLVSTQKQNQFDKHYLEMAAIWAKNSYCKRRQVGALIVKDKM
                     IISDGFNGTPEGFENECEDENDKTKAYVLHAEANAITKVAKSGNNSLGATLYATTSPC
                     IECAKLIIQAGIKRVVYLDDYRNDDGLKLLKRANIEVINFKDNE"
     gene            4979..6607
                     /locus_tag="PKN53_08720"
     CDS             4979..6607
                     /locus_tag="PKN53_08720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOD89069.1"
                     /translation="MSNFNNSKISSLLPLIIAASILFGIFVADFFSANNKNSSLLVPK
                     KIDKLSLLLDFIASEYVDSISNNELIELAIPKILESLDPHSIYITAEDAKSMQEELSG
                     DFEGIGIQFNMFKDTLLVVNVIKNGPSEKAGLKAGDKIIFVDSTKIAGVGIKNEEVIK
                     MLKGPAKTNVDLKILRKNSKRLLNFSIIRDKIPLYSVDAAYKLAPKTAYLKIAYFAAN
                     THSQFVDAMFTLQKTGLDTIVLDLRDNSGGYLTAATDILDEFFPKDIDLVYTEGRARQ
                     KQFVKSTSKRNYCQNMEIIVLINEFSASASEIVAGAIQDNDRGFIIGRRSYGKGLVQE
                     ATTFNDGSVIRLTTARYFTPSGRCIQKPYEHGADEYNLEVYNRFLNGEFLDKDSIKLN
                     DSLIFYTKNKRPVFGSGGIMPDIFVPIDTSGYTDAYGKISRNNLDYIFSIEYVKENRS
                     RLEKIKSTNELLRFLKKDAVLQKFYNYLSENNITVSQRDIRISGKYIENNLYAYIARQ
                     ILGDSSFYEISNTIDPVIDTVMNVIRDNRKLIEK"
     gene            complement(6619..7938)
                     /locus_tag="PKN53_08725"
     CDS             complement(6619..7938)
                     /locus_tag="PKN53_08725"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOD89070.1"
                     /translation="MNIIKHITNVRALQAFQLIRFVVLFLINIIFSKSNLKLGEIGTY
                     ETFLLIAGGVSFFWIGGMIQSLLGVYNNSETFGKSEKNSIFFNITLVFLFLSILSAIL
                     VFFSQDFISKLFSLNGHNIPYMKILFMYIVLSGPVNLVEYFYLLINKPIHIIVYGLST
                     FALQLVCVTLPILLGYDLGYGLYGLLFVNILRFGILLIIVYKYSELKFSKDFLKEFLK
                     LSAPLILGILLSGSAQYIDAFLVSYKFDEAKLAVFRYGAKEFPIFVLLIYAYSNAMTP
                     KFANKDNLQSALLELKQEGKKLMSWIYPSSILLILTSKFLYPIVFNPNFAESAVVFNV
                     YLMIIVIRFLFTRPIVIGFKETKVIFWSSFFETILNVTLSLILINVWGIVGVAVATLI
                     SYIFESLYLIIYLKRKHNILPNSYLSVKQYLFWTSLLIIAFVFSFYI"
     gene            complement(7961..8569)
                     /locus_tag="PKN53_08730"
     CDS             complement(7961..8569)
                     /locus_tag="PKN53_08730"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOD89071.1"
                     /translation="MFSGIVEETGKVVKIEKDQGNFHFFLTCSFADKLKVDQSLAHNG
                     VCLTVVNVDKANKIYQVTAIQETLDKSNLGLLKVGDEVNLERSMSMDKLLDGHLVQGH
                     VDQTAECVKVEESDGSWYFTFKYDKSRGNITVEKGSISVDGVSLTVVNSRDDRFSVAI
                     IPFTYEVTNFHNYKVGTIVNLEFDIIGKYIQKIMAGYLENKA"
     gene            complement(8612..9532)
                     /gene="queG"
                     /locus_tag="PKN53_08735"
     CDS             complement(8612..9532)
                     /gene="queG"
                     /locus_tag="PKN53_08735"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOD89072.1"
                     /translation="MKIPAEKIKNLAKEIGFSDCGITNILVEQEVKKFYKNWIENNFN
                     AEMKFLENEFEKRFEPKLVFPKAKSVIVFIINYYYKDYSSEEYKISKYAQGSDYHSVV
                     KDRLRQFSEILKQEYLDFESYEFCDTAPVFEKYFASQAGLGFIGKNRCLISPKFGSWV
                     FIGGIFCNAEIETDSKLDIYCGDCDICIKSCPTGALSSKGLDADKCISYHTIENKNEV
                     PENIRKKITNQLFGCDICQDSCPYNKNPIQTSCVEFLPQEKVYNLDFEELANISNKEF
                     SRLFKNTSLLRAGRKKIIINFENINYNRNK"
     gene            complement(9535..10641)
                     /locus_tag="PKN53_08740"
     CDS             complement(9535..10641)
                     /locus_tag="PKN53_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89073.1"
                     /translation="MKKYKITKILPILIILFCFKFQVKAQNISFFTDYLNNVLVFDNG
                     NLKQLEHLPVKSYVLGNNAFAYQDNAGNFKVYHNHFLHTLSSFVSSYTVSDNLIAWKM
                     NTQLKVFDNSSSLTLSIAATDCFVGDDIVVWFDDTQKKLKAYYEKEIYELDDALGTGT
                     MNGVFMGENIVAFVDSHGYLGVFYEGEIENLGYYDRIKSLKVGRDIIAFVEEPLNNFQ
                     VYYFGDVSDLESFEPKSYKVGDAFVAYVDANDYLKVFYNYKHETISFDKPNFYEVADE
                     VMVFGLQNYFKVWYKGKVFTLDSHIPQDYKMNNNIVVWIDPLGNLKLFDGEKVETISY
                     ETIDEFEIHGSCVKYKFGVNSENIYSNGKTYKND"
     gene            10739..11764
                     /gene="thiL"
                     /locus_tag="PKN53_08745"
     CDS             10739..11764
                     /gene="thiL"
                     /locus_tag="PKN53_08745"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOD89074.1"
                     /translation="MKKNQLNEISNLGEFALIDRLSKNFTITDKKLVKGIGDDAAVFE
                     ASKDNCFLVATDLLVEGIHFDLIYSPLKHLGYKAVAVNLSDIYAMNAKPKYITVSIAI
                     SKKFSVQALDLLYEGINLACERFDVELIGGDTTSSLSGLFINITVIGEAKKDEIVYRS
                     TAKKNDLICVSGDLGAAYMGLQILEREKEVFMQDPQIQPDLQGYDYIIERQLKPEPRR
                     DIINRLKEAGIKPNSMIDISDGLSSEVLHLCKQSNLGAVIYEDKIPINQQTAQAAEMF
                     RLLPLIPALNGGEDYELLFTVPLSEKEKIERVQDVSIIGYMHEDSNKKLLIPVNGEEI
                     ELKALGW"
BASE COUNT         4354 a         1935 c         1552 g         3974 t
ORIGIN      
        1 tggatatcca tccggcattt aagcatttaa gagataataa tttgataata aaaatgaaag
       61 tgatttttta atttcatgaa caaaatacat ttccatttca cctttatatt ttacatcaat
      121 ttttcctcga tattggcaaa caaaaaagtc tttaatccac ttataggtgc tttctgaaac
      181 attaacttta tttacaacac cataagattc cattctacta gcaatattta ccgtatctcc
      241 ccaaatatca tattctaatt tttgtgagcc taccactcca gcaaccacag gtccggtatg
      301 tactcctatt ctaatttcta acttcacatc actttcagca tttattttag taatttttct
      361 ttgcatttct aatgctgcca acactacttc aactggattt gtgctatctt tatttggcat
      421 acctccagca cacatataag cgtcgccaat agttttaatt ttctcaatat tgtatttagc
      481 aattatttta tcaaaactag taaaaagtct attcaaatgc tttattaatt cttcaggctt
      541 tgcctgtgct gcaatttttg taaaaccctg aatatcagca aacaaaacag taaccatttc
      601 atatttttgg gattgtgctt ttcctgtttc tcttaattcg tcggcagttg tttttggcaa
      661 caattttttt accaactgct ctgtttgttc tttttgataa gccaattcat ttgttctatc
      721 ttcaacaagt ttttcaagac tatatctttc gtgtgcatgt cttagtgcgt aaagttttaa
      781 tattaaaaaa actgctgtga ataaaatcaa aacataaaat aaaaaagcat accaagttct
      841 atacacaggt gctcgaatag taaaaaacaa gctggtaaca tcgctaatat ttccatattc
      901 atcttttgct cttatttgaa actcataatc tccttcaaat aaagatgtat attctttaaa
      961 attagcactc tgccaatctg accaatcatt gtcaaaacct actaatttaa tctgaaattt
     1021 tgcttgtcca aagctattgt aattaagtgc agtaaagtca aatctaattg ttttatctgc
     1081 aaatttaaat tcatattttt cctcaccact acaattaaaa ggcacatata ttaaagaatc
     1141 accatgaatt gttatttttc taattaagac attaatcttt ttttgataaa attttgtttt
     1201 ttcattatcg attttaatta atgcctctgt tgatccgagc caaacgtttc catcatcttc
     1261 gggatatata ctttctataa caaattcttt aagttgcgaa tatggacctg caacagcggt
     1321 ataacttgaa tttgagtctt gaaactcaaa taatcctgtt tctctctcat atctaccatc
     1381 agtaatattg ctataccaaa tatttttatt tttgtcttcg actaaaggat ataaataaga
     1441 atttgtttta gcataatttt tattaaacaa agtatcttca aaaaaatagt taccttgata
     1501 gctatatcta tacacacctt tatttgttgc aaacaaaact ccttgaaaag aaggaaaaac
     1561 atctatccaa tcataatctt cgggtaatcc tgttttttct gcaaaactaa ttatatctgg
     1621 gttaagattt aaccctgaga aaaagctaag ttgaaataat ccgtcttcac ttgttccaag
     1681 ccagaaattg ccaaaaaaat cttcacaaat agttctagtg ctataagaat aatttttcac
     1741 ataccctaga gtgtcaattt ttgaatcttc aactgaagct atcaacaatc catcttttga
     1801 ccctatcaaa atattatctg ggaaaaaact catttttgag atagacataa aattacctga
     1861 aagttgtaat ttagctttac taccatttat cagataaatt ccatcatttg taacggcata
     1921 caaattgcct tgaatttcaa caagcttcca acacattgcc ctaatttcgt ctattttttt
     1981 aaatttagct ttataatcaa aagagtttcg atttttatca taaacacttt ggcaattgta
     2041 caaataaaca ccattagttg ttgcaacata gaggtctgat tcaaatctaa ttacacttaa
     2101 aatcactcca ttaagaccat aataagagcc aaaaaaactt atattacttt ttgtttcgat
     2161 taagcttatt ccactaaaag tagttaacca taagtttcca tcattagcaa catacatact
     2221 tgttatctga ttgtctctca aactattatc tctactcatg gaaaaaacat aatgtccttt
     2281 ttcatcaaca caaacaatac caccatgttc tgtcccaaaa accattcttt tattgtctag
     2341 ggctaaacct accgaataag tattagcctg tataaaatca tctgcttctg ttttaaattc
     2401 agttatcgtt ccgtctgaat atttaaaaaa gctttcactt ttctgatctc taactaaaaa
     2461 ctcttttgaa tttaaagcta ttatatcaca tatagtcatg ttctcaaaaa gctcagaatc
     2521 ttcaaatttt acaaaatccc cattttgaca ataagacaaa cccttttctt gtatattaca
     2581 taatattttt tcattaaatt taaatatttt aacattagat tgaaaagttt taactgattg
     2641 caaactatct ctatgaaaat aaaacaattg ctttgaactc accacatata agtcattatc
     2701 aatagctata atttgcgtta cctgtccaaa tttatcatgt tccccaagat ttaatagttt
     2761 tgttaaaaga tgtccatttt cataaacaat tttcccaatt tgattataac ccccataata
     2821 aacttcatta tttgaattaa ttgtcaacat tggtttgcct ggaactttca ctaaacgcca
     2881 tgaactatta tcaaattcca taatcccatt agcatttcca aaatacatta tcccttcttt
     2941 attttgaaca acactaaaat tctgactttc ttgtccataa ttacgtggtg aaaagttttt
     3001 aaatccaggg atacctgttt caaaattttg acaaaaggca ttagaaacaa caaaaattaa
     3061 tactaatatt gcactaatat ttttcataaa atatttttaa caaacttaat tttaatttgt
     3121 taaaagacaa aatacttaaa ctaaattttt aaaaaaaatt ataaacatgg ctttagggaa
     3181 agagaataaa atacacatag gaatatatgg acgtagaaat gtaggtaaaa gcgctttaat
     3241 aaatttttta tctgggcaaa acatttctat tgttgcagat ttaccgggta caaccaccga
     3301 tccagtaaaa cgaagttttg aaattacagg atttggtcca gcaattttta ttgacacggc
     3361 tggaattgat gatgctggcg aaattggaga tttaagaaca aaaaaaactt tagatacaac
     3421 tgataaagtt gacttagcaa ttttggtatt tgattctaat acttttggca aatacgaaga
     3481 aagtttaata aacaacttta ataatgcaaa actgccatat attttactgc acagcaaatc
     3541 tgacatacaa tcacttaata cagatttaga acaaaaatta aaaaacaatt tcacaaaaaa
     3601 cattattgat ttttcaataa aagatttgac aaaaagagat gaattttttg aacttataaa
     3661 atcaacaatt cctgatttat ctttaaaaac tgatactctt ttaggcgata ttataaaaaa
     3721 aggggattta attttattag taagcacaat tgatgctggc acacctgctg ggcgattaat
     3781 tttacctcaa gttcaaacta ttcgcgatgt tttagacaat gatgctatat caattatttt
     3841 aaagcaagat gaaataaaag attttttaga aaaatcaaaa gtaaagccca acttagttat
     3901 tactgattct caaatttttg acacagttag caaactaata gataaaagta ttctattaac
     3961 tagtttttca attgttttag ctagacaaaa aggtgatttt caggcatata ttgaaggcac
     4021 accacatatt gaaaacttaa aagacggaga caatatttta attttagaat cttgtagtca
     4081 tcatgtttct tgcgatgata taggacgaaa gaaaattcca actttattaa gaaattacac
     4141 aaaaaaggaa ttaaacttta ctgttgttag tggtttagac aatattcctg aagcaattca
     4201 aaattatgct ttagttatac aatgtggtgg atgtgtgtta acaaaaaagc aagttcaaaa
     4261 taggctaaaa ccagcagtag atgccaaaat tccagtaaca aattatggta tgttaatagc
     4321 atatgtacac ggtatttttg aacgtgctgt gcaagtattt aaaaaatagt aaaaataatt
     4381 atttgtaatt taaaaataag gtattatttt tgctccgaat tgcccgggtg gcggaatcgg
     4441 tagacgcgct ggtctcaaac accagttcct tccctggagt gccggttcga ccccggcccc
     4501 gggtactgcc ctcaacaaag agggcttttt taaaaaatgt aaactatgaa taaactcgta
     4561 agcacacaaa aacaaaatca atttgacaaa cattacctcg aaatggctgc aatttgggca
     4621 aaaaactcat actgcaaacg aagacaagta ggcgctctta ttgttaaaga caaaatgata
     4681 atatcagacg gatttaatgg aacacctgaa ggttttgaaa acgagtgtga agatgaaaac
     4741 gacaaaacta aagcctatgt tttgcacgcc gaagcaaatg caataactaa agttgcaaaa
     4801 tctggaaaca atagtcttgg agcaacactt tatgcaacta cttcgccttg catagaatgt
     4861 gctaaactaa taatacaagc gggtattaaa agagttgttt atttagacga ttatcgtaac
     4921 gatgatggac tcaaactttt aaaaagagca aacattgaag ttataaactt taaagacaat
     4981 gagtaatttt aacaattcta aaatttctag cttactacct ctcattatag cagcatctat
     5041 tttgttcgga atttttgtag ccgacttttt tagtgcaaac aacaaaaact ctagcttact
     5101 agtaccaaaa aaaatagata agttaagttt attattagac tttattgcta gcgaatatgt
     5161 tgacagcata tcaaataatg aattaattga actagcaata ccaaaaatat tagaatcctt
     5221 agacccacac agcatatata ttaccgcaga agacgcaaaa agcatgcagg aagaactttc
     5281 tggagacttt gagggaatag gaatacaatt caatatgttc aaagatacac ttttagttgt
     5341 aaatgtaatt aaaaacggtc cttctgaaaa ggcaggtctc aaagctggtg acaaaataat
     5401 ttttgttgac tctaccaaaa ttgctggtgt tggtattaaa aacgaagagg taataaaaat
     5461 gttaaaaggt cctgctaaaa caaatgtaga tctcaaaatt ttacgaaaaa acagtaaacg
     5521 ccttttaaat ttttcaatta ttagagataa aattcctctt tacagtgttg atgctgctta
     5581 caaattagct ccaaaaacgg cttacttaaa aatcgcatac tttgctgcta acactcactc
     5641 tcaatttgtt gatgcaatgt ttactcttca aaaaactggt cttgacacaa ttgttttaga
     5701 ccttagagac aattcgggtg gatacctaac agcagcaaca gatattttag acgaattttt
     5761 ccctaaagac atcgaccttg tttatacaga aggtagagca agacaaaaac aatttgtaaa
     5821 atcaactagt aaacgcaatt attgccaaaa tatggaaata attgtactta taaatgaatt
     5881 ttctgcatct gcatctgaaa ttgtagctgg tgcaattcaa gataatgata gaggttttat
     5941 aattggcaga cgctcatatg gaaaaggatt agtgcaagaa gctacaactt ttaatgacgg
     6001 ttctgttatt agactaacaa ctgcaagata ttttactcct tcaggacgtt gcattcaaaa
     6061 gccttacgaa catggtgctg acgaatacaa ccttgaagtt tataatagat tcttaaatgg
     6121 tgaattttta gataaagaca gcattaaact taacgattcg ctaatttttt acacaaaaaa
     6181 taaaagacca gtttttggta gtggaggtat aatgccagat atttttgtac caattgacac
     6241 atctggatat actgacgctt atggcaaaat tagtagaaat aatttagact atatattttc
     6301 tatagaatat gtaaaagaaa atagaagccg attagaaaaa attaaatcta caaatgaatt
     6361 actaagattt cttaaaaaag atgctgtttt gcaaaaattt tacaactacc ttagtgaaaa
     6421 caatatcact gtttcacaaa gagacattag aatttctgga aaatatattg aaaacaatct
     6481 ttacgcatat attgcacgac aaattttagg agattcgtct ttttatgaaa tttcaaatac
     6541 aatagaccct gtgattgata ctgtgatgaa tgtaattaga gacaatagaa aattgataga
     6601 aaaataaaac ctcgaaaatt atatataaaa gctaaaaaca aatgcaataa tcaataagct
     6661 tgtccaaaac aaatattgtt ttaccgacaa ataagaattt ggcaaaatat tatgttttcg
     6721 ttttaaataa attatcaaat ataaactttc aaagatataa gaaatcaaag tagcaacagc
     6781 aacacctaca attccccata cattaattaa aatcaagctt agagttacat taaggatagt
     6841 ctcgaaaaaa gaagaccaaa aaatcacttt tgtttcttta aatccaatca caataggtct
     6901 tgtaaacaaa aacctaataa caataatcat caaataaaca ttgaaaacta ctgcactttc
     6961 ggcaaagtta ggattaaaaa caataggata taaaaacttg ctagttaaaa tcaacaatat
     7021 agaagatgga taaatccacg acatcaattt tttaccttct tgttttaact ctagtaaagc
     7081 actttgaaga ttgtctttgt ttgcaaactt tggtgtcatt gcattactat aagcatatat
     7141 tagcaaaaca aaaattggaa attcttttgc accatatcta aacaccgcaa gttttgcttc
     7201 atcaaattta taagaaacca aaaaagcatc aatgtactga gcagagccac tcaataatat
     7261 ccccaaaatt aaaggtgctg ataattttaa aaactctttt aaaaaatctt tggaaaattt
     7321 aagttctgaa tatttatata ctatgattag caaaattcca aaacgcaaaa tatttacaaa
     7381 aagcaaacca tacaagccat agcccaagtc gtatcccaaa agtataggga gagttacaca
     7441 aaccaattgc aaagcaaaag ttgacaatcc atagactata atatgtatag gtttattaat
     7501 taatagataa aaatattcaa ctaaattgac aggaccagac aaaacaatat acatgaaaag
     7561 aattttcata tatggaatgt tatgtccatt taaactaaat aatttggaaa taaaatcttg
     7621 actaaaaaat actaatattg cagaaagtat gcttaagaaa agaaaaacca atgtaatatt
     7681 aaaaaatatt gaatttttct cggatttgcc aaaagtttcg ctattgttat aaactcctaa
     7741 caaagattgt atcattccac caatccagaa aaaacttaca cctccagcta ttagcaaaaa
     7801 tgtttcgtat gtaccaattt ctccgagttt taaattactt tttgaaaaaa ttatattaat
     7861 caaaaataat actacaaacc taattagttg aaaggcttgt aatgcacgaa cattagtaat
     7921 atgttttatt atattcaatc tttgtatgtt gaataaaaaa ctaggcttta ttttcgaggt
     7981 agccagccat tattttttga atatattttc caataatatc aaattctaaa tttacaattg
     8041 tacctacttt gtagttatga aaatttgtta cttcgtaagt aaaaggaatt attgctaccg
     8101 aaaatctatc atctcttgaa tttacgactg ttaagctaac accatcaaca gagatagagc
     8161 ctttttctac tgtaatatta cctctcgact tgtcgtattt aaaagtaaaa taccaagaac
     8221 catcagattc ttcaactttc acacactcag cggtttggtc aacatgtcct tgaactaaat
     8281 gtccgtctaa caacttgtcc atactcatgc tacgttctag atttacttcg tcgcctactt
     8341 ttaaaagtcc taaatttgat ttatcaagag tttcttgaat ggcagtaact tgataaattt
     8401 tatttgcttt gtcaacatta acaactgtaa gacaaacgcc attgtgtgca agactttggt
     8461 caacttttag tttgtctgca aacgaacatg ttaagaaaaa atgaaaatta ccttggtctt
     8521 tttctatctt aactactttt cctgtttctt caactattcc tgaaaacata tttttacaat
     8581 ttttaaaaat tattctgcaa aactaataaa actatttgtt tctattataa tttatgtttt
     8641 caaaattaat aattatcttt tttcttccag ctcttaaaag tgaagtattt ttaaacagcc
     8701 ttgaaaattc cttgttagaa atatttgcaa gttcctcaaa atcaagatta taaacttttt
     8761 cttgtggtaa aaattccaca caagatgttt gtataggatt tttattatac ggacaagaat
     8821 cttgacatat atcgcaacca aacaattgat ttgtaatctt ttttcttatg ttttcgggaa
     8881 cttcattttt attttctatt gtatgataag aaatacattt gtcagcatct aaaccctttg
     8941 agcttaaagc tcctgttgga caagacttta tacaaatatc acaatctccg caataaatat
     9001 caagtttgct gtcggtttct atttctgcat tacaaaatat tcctccaata aaaacccaag
     9061 aaccaaattt aggacttatt aaacagcgat ttttcccaat aaaacccaaa cctgcttgac
     9121 ttgcaaaata tttttcaaaa actggagctg tatcacaaaa ctcataagac tcaaaatcta
     9181 aatattcctg ttttaaaatt tctgaaaact gcctcaacct atcttttaca acagaatgat
     9241 aatcagaacc ttgtgcgtat tttgaaattt tatattcttc tgaagaataa tctttataat
     9301 aataattaat aatgaaaaca ataactgatt ttgcttttgg gaaaactaat tttggttcaa
     9361 atcttttttc aaattcattt tctagaaatt tcatttctgc attaaaatta ttttcaatcc
     9421 agtttttata aaatttctta acttcttgtt caactaatat attagttatt ccacaatcgc
     9481 taaagcctat ttcttttgca agatttttaa ttttttcggc aggaattttc aagattaatc
     9541 atttttatag gtcttgccat ttgagtaaat attttcggaa ttaactccaa atttatattt
     9601 tacgcaagag ccatgaattt caaattcatc aattgtttcg taagaaatag tttcaacttt
     9661 ttctccatca aagagtttta aatttcccag agggtcaatc cacacaacta tattattgtt
     9721 catcttataa tcttgtggaa tatgactatc aagtgtgaaa acttttcctt tataccaaac
     9781 tttaaaataa ttttgaagtc cgaaaaccat tacctcatca gctacttcat aaaaattagg
     9841 cttatcaaag gaaattgttt catgtttata attgtaaaaa actttcaaat aatcattagc
     9901 atcaacataa gcaacaaaag catctcctac tttataagat tttggctcaa agctttctaa
     9961 atcagaaaca tcgccaaaat aataaacttg aaaattattt aaaggttctt ctacaaaagc
    10021 tataatgtct cttccaactt tcaaagattt tattctatcg taatatccta aattttctat
    10081 ttctccttca tagaacactc ccaaataacc atgagaatcg acaaatgcca caatattttc
    10141 gcccataaaa actccattca ttgtacctgt tcccaaagca tcgtctaatt cgtaaatttc
    10201 tttttcataa tatgctttta attttttttg agtatcatca aaccaaacaa cgatatcgtc
    10261 tcccacaaag caatctgtag ctgcaatgct caaagtcaaa gatgatgaat tatcaaaaac
    10321 ttttagttgg gtattcattt tccaagcaat taaattatca cttacagtat aagagctaac
    10381 aaaactactc aaggtatgca aaaaatgatt gtgataaact ttaaaatttc ctgcattatc
    10441 ttggtatgcg aaagcattat tcccaagcac ataagatttt actggcaaat gttcgagttg
    10501 ctttaaattt ccattatcaa aaaccaaaac attattcaaa taatcagtaa aaaacgaaat
    10561 attttgtgct tttacttgaa atttaaagca aaacagaatg attaatatgg gtaaaatctt
    10621 agtgatttta tattttttca taatcataat atttactttc aacaaaaata acgaaattta
    10681 atacactttt catatttttg cacaaataaa tttcaattat cttgccaaaa attaatttat
    10741 gaaaaaaaac cagttaaacg agatttcaaa tttaggcgaa tttgccttaa ttgataggct
    10801 aagcaaaaat tttactatta ccgacaaaaa attagtaaaa ggaattggtg atgatgctgc
    10861 tgtttttgaa gcaagcaaag acaattgttt tttagttgca actgatttat tggtcgaagg
    10921 tatccatttt gatttaattt attctccact caaacacttg ggttataaag ctgtggcagt
    10981 aaatttgagc gacatatacg caatgaatgc aaagccaaaa tatattactg tttctattgc
    11041 aattagcaaa aagttttcgg ttcaagctct tgatttactc tacgaaggaa ttaatttagc
    11101 ttgcgaaaga tttgatgttg agttaattgg tggcgacaca acaagctctt tgtcaggatt
    11161 atttataaac ataaccgtta ttggcgaagc aaaaaaagac gaaattgttt atcgttcaac
    11221 cgctaagaaa aacgatttaa tttgtgtaag tggcgactta ggtgctgctt atatgggttt
    11281 acaaatttta gaaagagaaa aagaagtttt tatgcaagac cctcaaatac aacccgattt
    11341 gcaaggctac gactatatta tagaaagaca acttaaacca gaacctagac gagatataat
    11401 aaatagactt aaagaagctg gaatcaaacc aaactcaatg attgacattt ctgacggttt
    11461 atcatcagaa gttttgcatt tgtgcaaaca gtctaattta ggagcagtaa tttatgaaga
    11521 taaaattcct ataaatcaac aaactgctca agctgccgaa atgtttagat tattaccatt
    11581 aattcctgct ctaaacggtg gcgaagatta tgaattgctt ttcactgttc cactatctga
    11641 aaaagaaaaa attgaaagag tacaagacgt aagcataatt ggatatatgc acgaagattc
    11701 taataaaaaa ttattaattc ctgtaaacgg cgaagaaata gaacttaaag cgttaggatg
    11761 gtgaatatca gttaaaagcg atgcactgaa cgtagttgaa gtgttaaaag ttgaa
//
LOCUS       DAOPNX010000081        11747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_6307_length_11747_cov_6.275145, whole genome shotgun sequence.
ACCESSION   DAOPNX010000081 DAOPNX010000000
VERSION     DAOPNX010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11747
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6307_length_11747_cov_6.275145"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..710)
                     /locus_tag="PKN53_08750"
     CDS             complement(126..710)
                     /locus_tag="PKN53_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOD89075.1"
                     /translation="MKKDIILAGVGGQGILTIAAIIGSTAVKKNLYLKQAEVHGMSQR
                     GGDVQSHLRISDKPIYSDLIAKGSADMILSVEPMESLRYLPFLKKDGYVITSTFTFKN
                     IPNYPEDEEVYAEIDKLPNKIALDAEQIAKDIENPRASNVVVLGAAAPFLGFTIEELE
                     GGIKDLFGNKGDDVVESNIKALHAGYNFAKSKIK"
     gene            complement(723..2324)
                     /locus_tag="PKN53_08755"
     CDS             complement(723..2324)
                     /locus_tag="PKN53_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOD89076.1"
                     /translation="MQKLLLLGDEAIAQGAIDGGISGFYAYPGTPSTEIMEYAQKSKE
                     VAQKGIHCKWSANEKTAMEAAIGMSYTGKRAMVQMKHVGMNVAADPFLNSSITGVNGG
                     LIVVAADDPSMHSSQNEQDSRFYGDFALVPILEPSNQQEAYEMAKNGFELSEKFNVPI
                     VLRVTTRLAHSRADIKLGDKKEQLKVKLPEDPRQYVLLPGIARRNYKKLLEMQSSIQQ
                     ASNDSIYNRYIDGNNKKIGIVACGIGFNYLMENFDGECPYPVVKVSQYPIPKNHIQKL
                     RKECESILVLEDGYPFVEKQLKSFFDEGIKIMGRLDGSLPRDGELNPNIVAKALGIKD
                     SETLQIPEVVKMRPPSLCQGCGHRDVYEALNIALEDYEKGRVFADIGCYTLGALPPFN
                     TVNSCVDMGASITMAIGASDAGLIPAVAVIGDSTFTHSGMTGLLDAVIKNSPILVLIS
                     DNFTTGMTGGQDSAAKNRIVDICKGIGVNPDHLRTIVPLKSNYEKNLAIIKEELAYNG
                     LSVVVAQRECIQTAKRGRKQQAKNK"
     gene            2577..4793
                     /locus_tag="PKN53_08760"
     CDS             2577..4793
                     /locus_tag="PKN53_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOD89077.1"
                     /translation="MKQFSIIIVPLKLFVFILLMAVPSQSFSAGTPSNGKKWKEAENL
                     LVSTKYFDAGKIYKEIYEAEPNHFNCAYKLGYCLLSSEKEQDVQGAIYYLRLASNNVS
                     EKYKNSYSEKKAPVISWYYLGVAYRLSGDYDEAIIAFKEYKTLQTKKEKKYFNSINVD
                     REIQTCKDAQEEIDYRRMSLEKIEIEGVEEGHLRCPVLAEATNRLIFTNGKNNVFPPD
                     INWKASYTDVVELDAVYMAVRDNVGGSFHSPQSIEADLQIKYPHVPVTVTADGSELYL
                     IVDKNDNGDIYMSKFENGKYQPAKKVKKLNTRKWESHATILPDGSRIYFASMRKGGYG
                     GLDIWYSDRDDEGKWKKPVNLGENINTKYNEEMPYIMNHGNALYFSSEGHTNIGGFDV
                     FYSNFDSQSQSWTKAQNLGFPFSTSGNDMGYIVENGKVFAFCPVNDNKRREGVGNCDC
                     ISLIRELVPELAEIRGIVTLNPEDAEKLQQVQVRLINSEDKTETARTKVDEDGKYLFE
                     NVKQGNYDIAIYINDEYQTSKQIDVLDGDNWNIQLDEIIVTLKDELIAETPEDNIDEK
                     IPAEKPKDNDDKKSIAENLEDNGEKTLQADDIEAVLYIENVFFDFDKSNVKSQYYSNL
                     DELANWLKANPEAELQISGHTDHYGTDEYNMGLSKRRAESVQKYLMNKGVKKSQLKIT
                     YHGKSQPLTIDVDDDTIRHLNRVVLFEVIKQGSPVIIVKPVIVPEGFEVKKTIVML"
     gene            4930..5220
                     /locus_tag="PKN53_08765"
     CDS             4930..5220
                     /locus_tag="PKN53_08765"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HOD89078.1"
                     /translation="MEENQKMLNPIEREEISHGVGTNKNRHLILYNDDINTFDYVISC
                     LIEICEHDFVQAEQCAFITHFKGKCDVKKGDYKSLKPMKQSLVNKGLKAAID"
     gene            complement(5268..6047)
                     /locus_tag="PKN53_08770"
     CDS             complement(5268..6047)
                     /locus_tag="PKN53_08770"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOD89079.1"
                     /translation="MEFKIITSELLDQYTAMVKESPLEKLEKMKKVEIPVDYFQFYKS
                     VSSVYSSKIEGEDIDFDSFFKHKFLKVKFKPDYTKKTDDLYAAYDFIENKKITLKNVQ
                     KAHSILSSNLLPKSQQGIIRTNPMLVINSNDQIEYIAASPEIVKTELNKLFDDIELLI
                     NTDLNPFEIFYYALFCHLVFVKIHPFQDGNGRTARLLEKWFLMNKLGQKATFVQLEKN
                     YYKQLKDYYANIRKLGLEYENLDYKKSLDFLVMTINGIEIE"
     gene            complement(6181..8256)
                     /gene="htpG"
                     /locus_tag="PKN53_08775"
     CDS             complement(6181..8256)
                     /gene="htpG"
                     /locus_tag="PKN53_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOD89080.1"
                     /translation="MLQKTGNIGVTTENIFPIIKKFLYSDYEIFLRELVSNAVDATQK
                     LRTLAISGDFKGDADDIKVEVILDKENKTLTIRDNGIGMSADEVDKYINQIAFSSAGD
                     FLDKYKNIENAIIGHFGLGFYSSFMVSEKVEIITKSYREDEKAVKWECSGDPAYTLTE
                     TEKETRGTDVILHIDEESLEFLEKNKIEELLHKYCKFLPTPIAFGKKTIWRDGKEEET
                     DEDNIINNPKPLWKQKPADLKDEDYKNFYNELYPASFEEPLFNIHLNVDFPFNLTGVL
                     YFPKIKSNLDINRNKIQLYCNQVFVTDSVEGIVPEFLTLLHGVIDSPDIPLNVSRSYL
                     QSDSNVKKISGHITKKVADKLFDIFKENREEFQTKWDDLKLFIEYGMISDEKFYERAE
                     KFALLKNTDDEYFTFEEYKTKIEAEQTDKNKTLIYLYATDKEEQFSYIKTAKSKNYDV
                     LLLDGQLDIHFINTLETKFKDSRFVRVDSDIIDNLIRKEDKIESVLSEREQDNLRWAF
                     QGAVDDDMKYWVSTENISGNDLPVLITRDEFMRRMKDMSALGGGTGYYRELPDHYKVI
                     INTNHETIIDLSSKIEKKNNSKRLEIDSQLDELNSEKSLIEDKLKDIKNEDDKPIEDK
                     KKIEELESKISELENKKRNLYKDFGKNQKIISQLFDLALLSNNLLKGEALSEFVQRSV
                     DLLKPKDKK"
     gene            complement(8336..8851)
                     /locus_tag="PKN53_08780"
     CDS             complement(8336..8851)
                     /locus_tag="PKN53_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89081.1"
                     /translation="MKSTKNITFYCVDNDTDDLDFIRENFKPNLNFNLLTFSSVQMFI
                     NKLKSEEKNKNFKIVLIDNIITSRGMNTRTALELLPPIKSIDKEIEVIIFADSENIEL
                     KATASNMRPAAFIKKDSQFFIRLYPIISRLISKYNLKKKQRTVRYTIIFLISLIVFAL
                     LFLIISNYILS"
     gene            complement(8863..9723)
                     /locus_tag="PKN53_08785"
     CDS             complement(8863..9723)
                     /locus_tag="PKN53_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89082.1"
                     /translation="MCKINSLYANELLNIEEDIDYIAELFIQIENEKSDTKKTEINQH
                     LIEYLQSFLAKSESFSADYSKIKKLSVLTSEDKLLKIYTWNLAFQNGSFKYFGFLQYK
                     PSGKINLFFLDDKKYNSENDTDPRLYQTNTEWYGAIYYEIVTKKWNSKTYYTLLGYDA
                     ADFLINRKVIETIYFDRKDLPIFGKKMFKINRTQTGRLIFEYADRVTMLLRYNKKQDM
                     IVLDHLVPPEPKYNNMYQFYGPDLSYDALVFRGGKWILETNIDPDVAINYKKDQKINH
                     IKRRGASKDF"
     gene            complement(9808..10992)
                     /locus_tag="PKN53_08790"
     CDS             complement(9808..10992)
                     /locus_tag="PKN53_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOD89083.1"
                     /translation="MISNRELFLNYLGQTSESPYLLEIESANGIYLIGPDKKKYIDLI
                     SGVSVSSLGHNFPPIKKAIEEQVQKHLHLMVYGEFIQSPQVKLAEYLVKLLPQEINSV
                     YLVNSGSEAIEGAVKLAKRYTGRTKMCSFVNAYHGSSHAALSLCGNEDFKKSFRPLLP
                     EVYILRINNYEDLNIIDEDTACVVIEPIQAEAGIIIPDKKWFKVLETKCKQNGTLLII
                     DEVQTGFGRTGKMFGFQHFDITPDIVCFAKALGGGMPIGAFVASKKIMHSFTTNPILG
                     HITTFGGHPVSAAAALAFLQHLNQSQLIEEVEEKEKLFRKNLKHQKIKEIRGKGLLLA
                     VEIENFDMVLKLVKQGLKDGFITDWFIFHDSAFRIAPPLNITKEEILLASKLIIESLN
                     KI"
     gene            complement(10985..11692)
                     /locus_tag="PKN53_08795"
     CDS             complement(10985..11692)
                     /locus_tag="PKN53_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD89084.1"
                     /translation="MNCIILDDDILTQKQLTSFIQKSDLLELKACFFNPKDVANFIEQ
                     NQIDIIFLDIEMPLMSGLEFLGQTNYNASVIIISGNKKYALNSYEYEVADYLVKPIEY
                     PRFLKAIYKIIEREHDKIKLDKTDRIFLKIAGKYERIKFSDIVFIQEINDCISITTKN
                     SIYIITCNKFKIEKFLQNKMFYKLDDEHTINLKEVTFISHTKVEFFNDFFLDDDIYID
                     STTLENIKNKIKEIEND"
BASE COUNT         3872 a         1738 c         1730 g         4407 t
ORIGIN      
        1 atgaatatcc attttggtgg ggtgttgcct tagcagtaag atatacgttc tagtttaaaa
       61 cattataagt taaaaaaaag gggctgtctc aaaaaacagc ctctttttta tttgtatgaa
      121 aaaatctatt ttatttttga ttttgcaaaa ttatatcctg catgtaatgc tttaatattt
      181 gattctacaa catcgtcgcc tttattacca aataaatctt ttattccacc ttcaagttct
      241 tctatggtaa aacctaaaaa cggagcagca gctcccaata caacaacgtt agaagctcta
      301 gggttttcta tatctttggc tatttgttcg gcatctaaag caattttgtt tggaagttta
      361 tcaatttcgg cataaacttc ttcatcttct ggataatttg gaatattttt aaaagtaaat
      421 gtgcttgtaa taacatagcc atcttttttt aggaaaggta aataccttaa agattccatt
      481 ggttctactg ataaaatcat atctgcacta ccttttgcaa ttaagtcaga ataaattggt
      541 ttatccgaaa ttcttaaatg agattgaaca tcaccgccgc gttggctcat tccatgaacc
      601 tcagcttgtt ttaaatataa gtttttcttt actgcagttg aaccgataat ggcagcaatt
      661 gttaaaattc cttgtccgcc tacgcctgct aaaattatgt cttttttcat gttcgtaaat
      721 ttttatttgt ttttagcctg ttgttttcta cctctttttg ctgtttgaat acactctcgc
      781 tgtgcaacaa caacagacaa tccattatat gctaattctt ctttaattat tgctaagttt
      841 ttttcgtaat tgctttttaa aggcactata gttcttaaat gatctggatt aacgcctata
      901 cctttgcaaa tgtcaactat tctgttttta gcagcagagt cctgtccgcc tgtcattcct
      961 gttgtaaaat tatcagaaat caaaactaaa ataggagagt tttttattac agcatctaac
     1021 aagcctgtca tgcccgaatg agtgaaagtt gagtctccaa ttactgcaac tgctggaatc
     1081 aatcctgcat cagatgctcc aattgccatt gtaatagaag ctcccatatc aacacaagaa
     1141 tttacagtat taaatggtgg taaagctcct aaagtgtagc aaccaatatc agcaaaaact
     1201 ctaccttttt cgtaatcttc tagagcaata tttaaagctt cgtaaacatc tctatgaccg
     1261 caaccttggc atagtgatgg tggacgcatt ttaacaactt caggaatttg tagtgtttca
     1321 gaatctttaa tacctaaagc tttagcaaca atatttggat ttaattctcc atctcttggt
     1381 aagcttccat ctaagcgacc cataattttt ataccttcat caaagaaact ttttaattgt
     1441 ttttcaacaa atgggtagcc atcttctaag actaaaatac tttcacactc tttgcgtaat
     1501 ttttgaatgt gattttttgg tataggatat tgtgaaactt taactactgg ataaggacat
     1561 tcgccatcaa aattctccat taagtagtta aagccaatac cacaagcaac tataccaatt
     1621 tttttattgt taccatcaat atagcggttg tatatagaat cgttagatgc ttgctgtata
     1681 gagctttgca tttctaaaag cttcttgtaa tttctacgag caataccagg taacagtaca
     1741 tattggcgag gatcttcagg taattttact tttaattgtt cttttttatc ccccaacttt
     1801 atgtctgcac gtgagtgagc taatctcgta gtaactctaa gcactattgg cacattgaat
     1861 ttttctgata gttcgaatcc atttttcgcc atttcataag cttcttgttg gttacttggt
     1921 tctaatattg gaactaatgc aaaatctcca taaaatctcg aatcttgttc gttttgtgaa
     1981 gagtgcattg atgggtcatc agcagcaaca acaatcaaac ctccattaac accagttatt
     2041 gaagagttta aaaaaggatc tgctgcaacg ttcatcccca catgcttcat ttgtaccatt
     2101 gctcttttac cagtataaga cattccaatg gcagcctcca ttgctgtttt ttcattggca
     2161 gaccatttgc aatgtatccc tttttgagca acttcttttg atttttgtgc atattccata
     2221 atttcagtag atggtgtgcc tggataggca taaaatcccg aaattccacc gtctatcgct
     2281 ccttgagcaa tagcttcgtc tcctaataat agtaattttt gcatatgtat atctttattt
     2341 tattttcaac tgtacgaaaa taagaaatta ttttttaaaa caaaaatata ttaaaagcta
     2401 tttttgaaat atgtttgaaa aacatatttt aatacaattt attacacttt aattacatgt
     2461 ttattacaat gtttttatga ttagtgtata ttttaatgct tttttttaat gaaataataa
     2521 aaaaaagtta tatttgtagt taatttttat atgtttataa taataaaata taaattatga
     2581 aacagttttc tataataatt gtacctttaa aattgtttgt ttttatactt ttaatggcag
     2641 ttccaagtca aagtttttct gcaggaacac catcaaatgg aaaaaaatgg aaagaagctg
     2701 aaaatttgtt ggtaagcact aagtattttg atgctggaaa aatttataaa gaaatatatg
     2761 aggctgaacc taatcatttt aattgtgcat ataaattagg ttattgttta ttgagttctg
     2821 aaaaagagca agatgtgcaa ggtgcaatat attatttaag gttagcttcg aataatgtaa
     2881 gtgagaaata taaaaattct tattctgaaa aaaaagctcc tgtaatttcg tggtattatt
     2941 taggtgttgc ttatcgtttg agtggcgact atgatgaagc tataatagct tttaaagaat
     3001 ataaaacgtt acaaacaaaa aaagaaaaaa aatattttaa ttcaatcaat gtagatagag
     3061 aaattcaaac ttgtaaagat gcacaagaag aaattgacta tcgtagaatg agtttggaaa
     3121 aaattgaaat agaaggagtt gaagaaggac atttacgttg tccagtttta gcagaagcta
     3181 caaatagatt aatttttact aatggtaaga ataatgtttt tccaccagat attaattgga
     3241 aagcatcata tacagacgtg gttgaattag acgcagtata tatggctgtg agagataatg
     3301 taggaggtag ttttcattcg ccacaatcta ttgaagccga tttgcaaatt aaatatcctc
     3361 atgtgcctgt tactgttact gccgatggct ctgaacttta tttaattgta gataaaaatg
     3421 ataatggtga tatttacatg agtaaatttg aaaatggaaa atatcaacct gcaaaaaaag
     3481 ttaaaaaact taatactaga aaatgggaat ctcatgctac aatattacca gatggttcac
     3541 gtatttattt tgctagtatg cgaaaaggcg gatatggcgg tttagatatt tggtatagtg
     3601 atagagatga tgaaggaaag tggaaaaaac ctgtaaattt aggagagaac ataaatacaa
     3661 aatataacga agaaatgcca tatataatga atcatggaaa tgctttgtat tttagttctg
     3721 aagggcatac aaatattgga ggttttgatg tgttttattc taattttgat agtcaatctc
     3781 agtcttggac taaagctcaa aatcttggtt tcccatttag tacatcaggt aatgatatgg
     3841 gatatatagt cgaaaatggt aaagtatttg ctttttgtcc tgtgaatgac aacaaaagaa
     3901 gagaaggggt aggaaactgc gattgtattt cacttataag agaacttgtg cctgaattgg
     3961 ctgaaattag aggaatagta acattgaacc ctgaagatgc tgaaaaatta caacaagtgc
     4021 aagtaagatt aattaatagt gaagataaaa cagaaactgc tcgtacaaaa gttgatgaag
     4081 atggaaaata cttgtttgaa aatgttaagc aaggcaatta tgatatagct atttatataa
     4141 atgatgaata ccaaacttct aaacaaatcg atgtgcttga tggcgataac tggaatatcc
     4201 aactagatga aataattgta acacttaaag acgagcttat tgcagaaact ccagaagata
     4261 atatagatga aaaaatacct gctgaaaaac caaaagacaa tgatgataaa aaatccattg
     4321 cagaaaatct agaagataat ggtgaaaaaa cattgcaagc agacgacata gaagctgtat
     4381 tgtatataga aaatgtattt tttgacttcg ataaatctaa tgttaagtct caatactatt
     4441 ctaatttaga tgaattagca aactggctta aagcaaaccc tgaagcagaa ttgcaaattt
     4501 ctggacatac cgaccattat ggtactgatg aatataatat ggggttaagc aaaagaagag
     4561 cagaatcggt tcaaaaatat ttgatgaata aaggtgtaaa aaaatctcaa ctcaaaataa
     4621 cttatcacgg aaaatctcaa ccactaacaa tagatgttga tgatgataca attagacatc
     4681 ttaatagagt tgtgctattt gaagttatta aacaaggcag ccctgtaata atagtaaagc
     4741 ctgtaatagt gcctgaagga tttgaagtta aaaaaactat agtaatgctc taagttttgt
     4801 tgtgtaaaac gttttttaat agagagtttt atttggttgg ttagtgaaat tgtgcatatt
     4861 ttataaaata ggctttctaa aactttttat ttagctttat gtcgttttaa ttgattactt
     4921 ttgcattata tggaagaaaa tcaaaaaatg ttaaacccaa tagagcgtga agaaatatca
     4981 cacggggtag ggacaaataa aaaccgtcat ttaatattat ataatgatga tataaatact
     5041 tttgattatg tgatttcttg tttaatagaa atctgtgagc atgattttgt ccaagccgaa
     5101 caatgtgctt ttataactca ctttaaagga aaatgtgatg tcaaaaaagg tgattataaa
     5161 tcattaaagc ctatgaagca aagccttgta aacaaagggc ttaaagcagc aattgattag
     5221 aaatcagtca ggcaaaccat aatattataa aaatcttttt taacttatta ttctatttca
     5281 atcccattta ttgtcattac aagaaaatca agactttttt tatagtccaa gttttcgtat
     5341 tccaagccta attttctgat gttagcataa taatctttaa gttgcttata ataatttttt
     5401 tctagttgga cgaatgttgc tttttgtcct aatttgttca ttaaaaacca tttttcaagc
     5461 aatctagccg tacgtccatt accatcttga aaaggatgaa tctttacaaa tacgagatga
     5521 caaaacaatg cataataaaa tatttcgaaa ggattcaaat ctgtatttat aagtaattca
     5581 atatcgtcaa aaagtttgtt taattcagtc tttacaattt caggacttgc tgcgatatat
     5641 tcaatttggt cattactatt aattacaagc attgggtttg ttcgaattat tccttgctgg
     5701 cttttgggta aaagattaga acttaaaatt gaatgtgctt tctgaacatt ttttaatgtt
     5761 atttttttgt tttcaataaa atcatatgca gcatataaat catcagtttt ttttgtgtaa
     5821 tctggtttaa actttacttt taaaaattta tgcttgaaaa aactatcgaa atcaatgtcc
     5881 tctccttcaa ttttagaaga ataaaccgaa gatactgatt tgtaaaactg aaaatagtca
     5941 acaggaattt caactttttt catcttctcc aatttttcta atggagattc tttaaccata
     6001 gctgtatatt ggtcaagtaa ttcagatgtt attattttaa attccataaa tcttttgcaa
     6061 aaatagagtt ttgtttaaaa attatattac caaaagtaat gatttttttc taaaaataaa
     6121 aaaaccagct aaatcatttt gaattagctg gtttttatct tatgaaaaaa tgttgtgtta
     6181 ttatttctta tctttaggtt ttaacaaatc aacactacgt tgcacaaatt cactaagagc
     6241 ttcacctttt aatagattat tagaaagtaa tgctaaatca aagagttgag aaataatctt
     6301 ttgatttttg ccaaaatctt tatataaatt tcgtttttta ttttctaatt cagaaatttt
     6361 cgattctaat tcttcaattt ttttcttgtc ttctattggc ttatcgtctt cgtttttaat
     6421 gtctttgagc ttgtcttcta ttaaagattt ttctgaattt aattcatcta attgagaatc
     6481 aatttctaag cgttttgaat tgtttttctt ttcaattttt gagcttaaat caataattgt
     6541 ttcatgattt gtattaataa ttactttgta atggtcaggt agttcgcggt aatatcctgt
     6601 tcctccgcct aatgccgaca tatctttcat tcttctcata aattcatcgc gagtaattaa
     6661 aacaggtaaa tcatttccgc taatattttc tgtactaacc caatacttca tatcatcatc
     6721 aacagctcct tggaaagccc accttaaatt atcttgttca cgctcgctaa gaacagattc
     6781 tattttatct tcttttctaa ttaagttgtc gataatgtcg gaatcaacac gtacaaaacg
     6841 cgaatcttta aatttagttt ctaatgtatt tataaagtga atatctaatt gtccatctaa
     6901 taaaagtaca tcgtaatttt ttgatttcgc agttttgatg tatgaaaatt gctcttcttt
     6961 atcagttgcg tataggtaaa ttaaggtttt gtttttatca gtttgctcag cctctatttt
     7021 agttttgtat tcttcgaaag taaaatactc atcatctgta ttttttagta gagcaaattt
     7081 ttctgcacgt tcgtaaaatt tttcgtcaga aatcattccg tattcgataa ataattttaa
     7141 atcgtcccat ttagtttgga attcttcacg attttcttta aaaatatcga atagtttatc
     7201 agcaactttt tttgttatat gcccagatat ttttttaaca tttgaatcgc tttgtaagta
     7261 agagcgtgaa acatttagcg gaatatcagg tgaatctata acaccgtgta gtaaagtcaa
     7321 aaactctggg acaatgcctt caaccgaatc agtaacaaaa acttgattac agtatagttg
     7381 tattttgttt ctattaatat ctaagttaga ttttattttt gggaaatata atacaccagt
     7441 taaattaaaa ggaaaatcaa cattcaaatg tatgttgaaa agtggttctt caaaacttgc
     7501 tggatataat tcattgtaaa agtttttgta atcttcatct tttaagtcgg ctggtttttg
     7561 tttccacaat ggttttgggt tatttataat attatcctcg tcggtttctt cttctttgcc
     7621 gtctctccaa attgtttttt tgccaaaagc tataggagtg ggtaaaaact tgcaatattt
     7681 gtgtagcaat tcttcaattt tatttttctc tagaaactca agcgattctt cgtcaatgtg
     7741 taaaataaca tctgttcctc ttgtttcttt ttcagtttca gtaagtgtat aggcagggtc
     7801 gccactacat tcccatttta cagctttttc atcttcgcgg taagattttg taataatttc
     7861 tactttttct gaaaccataa acgaagaata aaatcctaaa ccaaaatgtc ctataattgc
     7921 attttcaata tttttgtatt tatctaaaaa gtcgcctgca ctcgaaaaag caatttgatt
     7981 tatatattta tcaacttcgt cggcactcat tccaatgcca ttgtcgcgaa tagtcaatgt
     8041 cttgttttct ttgtctaata tgacttctac tttaatatca tcagcatctc ccttgaaatc
     8101 acctgaaata gctaaagttc ttaatttttg agtagcatca acagcattgg atactaattc
     8161 tcgtaaaaat atttcgtaat cagaatataa aaatttttta attatgggaa aaatattttc
     8221 ggtagttaca ccgatatttc cagttttttg taacatatct tatcaatttt aatttaatcg
     8281 ataatttata ctcaaactat atgccactgg atttttgctg acaaaatgtc acagtttacg
     8341 acaatatgta atttgatatg attaaaaata ataaggcaaa aacaattaaa ctaattaaaa
     8401 atattattgt atatcttact gttctttgtt ttttctttaa gttgtattta gaaatgagtc
     8461 gcgaaatgat agggtagagc ctaataaaaa attgggaatc ttttttaata aaagctgcag
     8521 gtctcatatt gctagcagtt gctttaagtt caatattttc tgaatctgca aaaattatta
     8581 cttctatttc tttatctatg cttttaattg gaggaagtag ttctaatgca gtacgtgtgt
     8641 tcattcctct gcttgtaatg atattatcaa ttaaaacgat tttaaaattt ttattttttt
     8701 cttcagattt tagtttgttt ataaacattt gcacagaact aaaagttagc aaattaaagt
     8761 ttaaattggg tttaaaattt tctctaataa aatctaagtc atctgtatca ttatcaacgc
     8821 agtaaaaagt tatatttttt gtagatttca ttttttttag atttaaaaat ctttagaagc
     8881 tcctcttcgt tttatgtgat taattttttg atctttttta taatttatag caacatcagg
     8941 gtctatattt gtttccagaa tccattttcc acctctaaat actagtgcat cgtatgataa
     9001 atcgggtcca tagaattgat acatattatt atatttaggc tctggtggaa ctaaatggtc
     9061 gagaactatc atatcttgct ttttattata tcttaacaac atggttactc tgtcggcata
     9121 ttcaaatatc aatcgtcctg tttgagttct atttattttg aacatttttt tcccaaatat
     9181 aggtaagtct tttctatcaa aatatatggt ctcgataact tttctattta ttaaaaaatc
     9241 tgctgcatca tagcctaaaa gtgtatagta agtttttgag ttccattttt ttgtaacaat
     9301 ttcataataa atagctccat accattctgt atttgtttga tatagcctag gatcggtatc
     9361 attttctgaa ttgtactttt tatcatctaa gaaaaataaa tttatttttc cagaaggttt
     9421 gtattgtaag aatccaaaat atttaaaact accattttga aaagccaagt tccatgtgta
     9481 aatttttaat agtttatctt cagatgttaa aaccgaaagt ttttttattt tgctataatc
     9541 tgccgaaaaa gattctgatt tagctaaaaa agattgtaga tattcaatca aatgttgatt
     9601 aatttctgtt ttttttgtgt ctgatttttc gttttctatt tgtatgaaca actcggcaat
     9661 atagtcaatg tcttcttcta tattcagtag ttcgttggcg tacagactat ttatcttgca
     9721 caaaaaacaa attattaatg atattttaaa aactaacttc atcaatatta ataacgcaaa
     9781 tatgcaattt tattttctaa aaagagatta aattttgttt aagctttcaa taattagttt
     9841 tgatgctagt aatatttctt cttttgttat gtttaaaggt ggtgcaattc taaatgcgga
     9901 gtcgtgaaaa atgaaccaat ctgtaataaa accatctttt aaaccttgtt ttactaattt
     9961 taaaaccata tcaaaatttt caatttcaac cgctaatagt aagcctttgc ctctaatttc
    10021 tttaattttt tgatgtttta gattttttct aaaaagtttt tctttttctt caacttcttc
    10081 tattagttgt gattggttta ggtgttgtaa aaacgctaga gcagcagcag ccgacacagg
    10141 atgtccgcca aaagtggtta tatgtcccaa aattggattt gttgtaaaac tgtgcataat
    10201 ttttttggag gcaacaaaag cacctatagg cattccgcct cccaaagctt tagcaaagca
    10261 aacaatgtct ggtgtaatat caaaatgttg aaaaccaaac attttcccag ttctaccaaa
    10321 tcctgtttga acttcgtcaa taattaatag tgtcccattt tgtttgcatt tagtttctaa
    10381 tactttaaac cattttttat ctggaataat tatgccagcc tcggcttgta tgggttcaat
    10441 aacaacgcaa gcagtgtctt cgtcaataat atttaagtct tcgtaattat taattcttaa
    10501 aatataaact tctggtaata gtggacggaa agatttttta aaatcttcgt tcccacataa
    10561 acttaatgct gcgtgcgagc taccatggta agcatttacg aaagagcaca tttttgttct
    10621 acctgtatat ctttttgcaa gttttactgc tccttcaatt gcttcgctac cactgtttac
    10681 taaataaact gaatttattt cttgaggtaa aagtttaacc aagtattcgg ctaatttaac
    10741 ttgaggggac tgaataaatt ctccatatac cattaaatgt aaatgctttt gaacttgctc
    10801 ttctatagct ttttttatgg gaggaaagtt atgccctaaa ctgctaacag atactccaga
    10861 aattaaatct atatatttct ttttgtcagg tccaattaaa tatattccat ttgcactctc
    10921 tatttctaat aaatatggag attcggaagt ttgtccgaga taattgagaa ataactctct
    10981 gttactaatc attttctatt tctttgattt tgttttttat attttctaat gtcgtagaat
    11041 caatatatat atcatcatct aaaaagaagt cattaaaaaa ttctactttg gtgtgtgaaa
    11101 taaaagtaac ttcttttaaa ttaattgtat gctcatcatc taatttataa aacatcttgt
    11161 tttgcaaaaa tttctcaatt ttaaatttgt tacaagttat tatataaata ctatttttag
    11221 tcgttatgct tatgcaatca ttgatttctt gaatgaatac tatgtctgaa aattttattc
    11281 tttcatattt accagcaatt tttagaaata ttctatcagt tttgtctaat ttaattttat
    11341 cgtgttctct ttcaataatt ttgtaaatag cttttaaaaa gcgagggtac tctataggtt
    11401 ttacaagata atcggcaact tcgtattcgt aagagtttaa agcatatttc ttgttccctg
    11461 atattataat gactgatgcg ttataatttg tttgtcccaa aaattctagc ccactcatta
    11521 ggggcatttc gatatctaaa aatattatat ctatttgatt ttgttcaata aagtttgcaa
    11581 catctttagg attaaagaaa caagctttaa gctcaagtaa atccgatttt tggataaaac
    11641 ttgttaattg cttttgcgtt aatatatcgt catctaaaat tatgcaattc ataaacttcc
    11701 ttaaatccgc aactaattat ctagaattaa aaacaaagcc ttgcaaa
//
LOCUS       DAOPNX010000082        11677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_6357_length_11677_cov_6.522802, whole genome shotgun sequence.
ACCESSION   DAOPNX010000082 DAOPNX010000000
VERSION     DAOPNX010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11677
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6357_length_11677_cov_6.522802"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..1481
                     /locus_tag="PKN53_08800"
     CDS             87..1481
                     /locus_tag="PKN53_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOD89085.1"
                     /translation="MKKTLSILTLFFLAQVVFAQSGNLDNNTLNIIRSNYSQKAENDI
                     AIRNALTNNDIRNLALNRDVVGKETHDFKYKVKVKGITDQKSSGRCWMFTGLNILRPQ
                     LIDEKKLSNFEFSTNYLYFYDMLEKSNLFLEGVLKYHDKEMDDRTVDWFFKDPITDGG
                     VWNSFANLVNKYGLVPKTVMPETFHSENTRWLNNILSSKLREFGLELREIRRQGITGK
                     KLQSAKMEMISEIYRILVLFLGEPPTEFTYRFVDKDNNISEAKTYTPQEFFKEIFPNY
                     NTEDYYMFMNDPTREYYKVYEIDFDRNVLEGKNWIYLNLPSDEIKTFALNSIKNNEAL
                     YASCDVGKQLYSAEGTLDVKNYNFEALLGTKFKMDKAARIKTYDSGSAHAMLLMACDT
                     DKDDKPTKWQFENSWGSSHGHNGYLTFTDEWFDEYMFRVVVNKKFIDDKTLKLFDQKP
                     ILLPPWDPMFSNDN"
     gene            1494..3368
                     /gene="asnB"
                     /locus_tag="PKN53_08805"
     CDS             1494..3368
                     /gene="asnB"
                     /locus_tag="PKN53_08805"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOD89086.1"
                     /translation="MCGIAGIFSFDKASTEYSKYLEKSISALIKNKNEKPNLFIEDNI
                     AFAYLKNTQHAENQNNIVISDDKNYLLLLEGDIFYTENLKNLINNTDKIKLKTDSEIL
                     LFLLIEHGESIIEKLNGNFVFAFFNKITNTLIVSRDYIGFKNLFFYKDDEKFIFSTEL
                     KSILQFDITKEINSAALFSYLQLNYILPNVSIIKNINKLGYGSFLKINKQGFEIKNYY
                     KIIPSFNPENYDNFEIASSKLRQLLSDAIEDNLAKHPDASCFLSGGIDSSLITALASK
                     FKKNLNTFSIGYKDVDFFDETQFAELVAQKYNTNHTSFKITNQELLDNIDSAFDNFDE
                     PFADSSALALNILSKHVSQHSSVVLSGDGADEMFAGYNKHSAHFQASYPGTKEKIAAG
                     LNPIWKIVPKSRSSKMSNLARQLDRFATGYGLNPNDRYWFWATIGTEEYAKNILKLPI
                     DYSQFSAKKHKYINQDICLKDINNVLLTDMNLVLQGDMLIKVSTMSAGNRLKIFAPFL
                     DKNIIDYSFSLPSHFKINSTSRKRILKNAFNDLLPDEIFNRPKHGFEVPLLMWFKKDL
                     KHLIEDKILSEDFIKEQGIFNYKEISSLKQKLNSPNPHDSPAKIWAIIVFQKWWTKHM
                     "
     gene            3372..4568
                     /locus_tag="PKN53_08810"
     CDS             3372..4568
                     /locus_tag="PKN53_08810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD89087.1"
                     /translation="MAKVLRIINRFNLGGPTYNVAYLTKFLAPDYDTLLVGGSNTKDE
                     ACSDYIIKKLGIHAITIPEMSREIGYNDLIAYAKIKNIIKRYKPDIVHTHASKAGYLG
                     RRAAISLKVPVIVHTFHGHIFHSYFNKQATSLFKSLEQSLAKKTDAIIAISPIQKDEL
                     CNIHKIAPEEKFRIIPLGFDLNRFQENLQEKRKIFRTRYNLKDDDIVISSVGRLVPIK
                     NHPLFIKSIAKVKEKTNKNVKAMIVGDGSLRNEFVELAHSLNLNVSTPEKTEPNPDII
                     FTSWIEDADIVFAGSEIAALSSFNEGTPVSLIEAQASSIPVISTDVGGVKDIIIEGKT
                     GLLCKNDDVDDYAEKLLILIENDKLREEMSLKGWEFVKNKFHYSRLVNDVKLLYDELL
                     KNSQIK"
     gene            4650..5381
                     /gene="gldF"
                     /locus_tag="PKN53_08815"
     CDS             4650..5381
                     /gene="gldF"
                     /locus_tag="PKN53_08815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HOD89088.1"
                     /translation="MFTLFFKEIKEFFSSVTGYLVVIIFLLINSMFLWIFKGVFNIFD
                     LEMANIDSLFIISPWVFLFLVPAITMNMFAGEYKSGTMELLLTRPLTEFQIVFAKYLA
                     SVVLVLVALAPTLLYLFSIYFLADPVGNVDFGAIFGSYIGLFFLAAIYSALGIFASSL
                     TKNIIISFLLGLLFCYVFFLGFENLSYLISSGKLSNFIISLGINDHYRSMSRGVIDSR
                     DVVYFLSVISIFIFVTKLRLESRKW"
     gene            5387..7153
                     /gene="gldG"
                     /locus_tag="PKN53_08820"
     CDS             5387..7153
                     /gene="gldG"
                     /locus_tag="PKN53_08820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HOD89089.1"
                     /translation="MENNNKKNKTSRSTLKKQNILQAILILVLIIVFNLLSSMVFFRL
                     DLTKEKRFSITDTSKKILDDLEDKIIIDVYLEGDDLPVELLRYQKTVKEFLDNFKSYS
                     NKIEVIFKNPFEEGNSEQNREIYFQLYKKGLSPTYISQSQDMGRSEKMIFAGALLKYG
                     EKEFPINLINNNLSSGSNYVGLSETELERDFIHAIWMLTNDKLKKIAFLDGHKELSEY
                     QTYDIMTSLSKYYHIDRVIMNQVLNALDGYDVVVVAKPEGYFTERDKFILDQYIMQGG
                     KMIWLVEWMKIDMDSLSTKPMQMAMLNDINLDDQLFNYGVRINPDLIQDLSCLSIPVN
                     TVDGKPQFESRPWPFFPVIVPDTLLNHQLLKNLDIIRTHFVSSIDTVGENPKIKKTIL
                     LRTSKFSKTMTHPVEVNLEIMRNEPELKTFNKPNLPIAVLLEGQFRSNFVNRLSDEFA
                     NNPDFKFIPESEETKMIVISDGDFIRNEVRVMGNKQQPYPLGADKYYPEQFTPGNTQF
                     FVNCVNYLCADDDFISLRMREIKIRKLNKAELRENKQHWLYFNLTLPILSIIIIGVVI
                     LFSRKIKFNKKPLKIHKTYSKK"
     gene            7150..8172
                     /locus_tag="PKN53_08825"
     CDS             7150..8172
                     /locus_tag="PKN53_08825"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOD89090.1"
                     /translation="MKKTNLILLIIVLVLVIVTASIYFFQNTSSQPAYRDFAIKDTAS
                     IDKIFIADKNSNTVLLERKDGYWEANKSFVARRDLTNLLLETFYRIEVSSPVPNSKLD
                     KVLKDLSVSGVKCEIYQNGKLSKTYYIGGVTDDNIGSYMIMEGSDQPFIVRIPGFNGF
                     LNVRYNTNPNEWKEKLIFNYKVQDIAKVTVEYSNSPDESFIAISKGNNEFDLKEINGS
                     KVKFDFDTIKVKTYISSCKLIGFDAYISDSLQQEKYDSLSVLPFVAKYTIEDIHGNAK
                     SFKTYLRQNITKLLDDEGNLYEWDIDYLYGILDDKTVVLLQYYVIDPIATGKYEFIKN
                     TEEHDY"
     gene            8269..9399
                     /gene="dnaN"
                     /locus_tag="PKN53_08830"
     CDS             8269..9399
                     /gene="dnaN"
                     /locus_tag="PKN53_08830"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOD89091.1"
                     /translation="MNFVVSSANLLGQLQLVSKVVVSKNALPILDNLLFDIKDGILTV
                     TGSDLETTIQTKIELTSCEGEGSIAIDTKKILEILKELPDQPLTFQINVEEQKIDITS
                     SNGQFSMTGQLGDDFPVIPSIKSEKEKKFDIMPDILYGGISSTIFATGDDELRPIMNG
                     ILIEFKNDKVSFVATDAHKLVKYSRKELKTDEEVSFILPKKPANLIKGILPKVFSDVE
                     LTFDDKNALFTFENTKLICRLIEGTYPNYATVIPKDNPNKLIVDRLELFSSLKRVSIF
                     ASQSSSLVGLEFTGNTLEISAKDTDYSVAGNETINCQYFGDNMKIGFKSNFLMEILSN
                     ISTQEVIFELSDPTRAGIILPKDNENENEDVLMILMPMMLNN"
     gene            9465..10235
                     /locus_tag="PKN53_08835"
     CDS             9465..10235
                     /locus_tag="PKN53_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOD89092.1"
                     /translation="MNIKLKKPIVFFDLETTGINPATDRIVEISILKVFPNGNETIKT
                     FLINPEINIPSEASAIHGITNDDVKDAPTFKMLAKEIVSLMDDSDLAGYNSNKFDIPL
                     LAEELLRAGSDFDLKKRNFIDVMVIFMKKEPRNLEAAYKFYCNKELENAHSAEADIKA
                     TYEVFKAQTHHYDDLNTDVESISKYSSQNKYVDFAGRLIRDENDNVIINFGKHKGKNI
                     NHVFEKDTAYYDWIQKNDFPLYTKKMFTELYIKFKQNK"
     gene            10255..11340
                     /locus_tag="PKN53_08840"
     CDS             10255..11340
                     /locus_tag="PKN53_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89093.1"
                     /translation="MQRYISVLLLIFITNLSFAQNHQTPDWLQDLPYNTSEYIYAIGF
                     SDPKMSDSILAKNLALNRALAIAVMVNQSQVSYASDFYEAKSEEHRWYITKESFEEFG
                     KIKASAYVNNNYEIVKSETNSNGELIILLKFFPSQEKGVEHNFNIEIEYYSQVFELSN
                     TRALEHFRNIMLNTCWTNLKTKDSLKTYYELSNYNNYINIKESYGTLEIEAPRFHYNY
                     KPSLANKIDFKDFSSYSPLQKGLWQSYLDSYTQSIFNINKNHSSKIGTIQDEFKRSKN
                     TYDSQQGQGQKTESLTRNTLRNTLNFELGGIEIYNNYLYNRVYLQNKRPSYSNVNSSQ
                     IKNENSNNAKDKKSKEWFFKRWFKKKS"
     gene            complement(11470..>11677)
                     /locus_tag="PKN53_08845"
     CDS             complement(11470..>11677)
                     /locus_tag="PKN53_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005809831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOD89094.1"
                     /translation="YKIINELFFWVSQCGKQDGNSHFQKHKIFLYKEDFGKFIEGYEQ
                     ALSYIKEQKPEYFEEQDLETEEEN"
BASE COUNT         4542 a         1664 c         1686 g         3785 t
ORIGIN      
        1 tatatctttg tagaaacttt aaaacattat ttaataatta ttgttgtatt tatttaatta
       61 gatttgattt ataaataaaa aataagatga aaaaaacact atcaatttta actttatttt
      121 ttttagctca agttgttttt gcacaatcag gcaatttaga caacaacaca ttaaatatca
      181 taaggagcaa ttattctcaa aaagccgaaa atgatattgc aattagaaat gcgttaacaa
      241 acaacgacat tagaaattta gctcttaaca gagatgttgt tggtaaagaa actcacgact
      301 ttaaatataa agtcaaagtt aaaggaatta cagaccaaaa atcttctgga cgttgttgga
      361 tgtttacagg tttaaatatt cttagaccac aacttataga tgagaaaaaa ttatcaaatt
      421 ttgaattttc tactaactat ctatatttct acgacatgct agaaaaatca aatttatttc
      481 tcgaaggagt tttaaaatat catgataaag aaatggacga tagaacagtt gactggtttt
      541 ttaaagatcc tatcacagac ggaggtgtgt ggaactcttt tgcaaattta gttaataaat
      601 atggacttgt tccaaaaact gtaatgccag aaactttcca ttctgaaaac acacgctggc
      661 ttaacaatat actaagctct aaacttagag agttcggctt ggagttgaga gaaataagaa
      721 gacaaggaat tactggcaaa aaactacaat ctgctaaaat ggaaatgatt tctgaaattt
      781 acagaatact agttcttttc cttggcgagc cacctacaga gtttacatac agatttgttg
      841 acaaagacaa taatatttca gaagcaaaaa catatactcc tcaagaattt tttaaagaaa
      901 tttttccaaa ttataatacc gaggattact acatgtttat gaacgaccca acaagagaat
      961 actataaagt ttacgaaatt gattttgaca gaaatgtttt agaaggtaaa aactggattt
     1021 atttaaatct accttctgat gaaataaaaa cttttgcttt aaatagcatt aaaaacaacg
     1081 aagctcttta cgcttcatgc gatgtaggaa aacaacttta cagtgctgaa ggaacattag
     1141 atgttaaaaa ttataatttt gaagctttat taggtactaa atttaaaatg gataaagcag
     1201 caagaattaa aacttacgat tctggttctg ctcatgcaat gttgctaatg gcttgcgata
     1261 cagacaaaga cgacaaacca acaaaatggc aatttgaaaa ttcatgggga agcagtcacg
     1321 gacataatgg gtatttaacc ttcactgacg aatggtttga tgaatatatg tttagagttg
     1381 ttgtaaataa aaaatttatt gacgataaga ctttaaaact tttcgaccaa aagccaattt
     1441 tactaccacc ttgggatcca atgttctcaa acgacaacta acaacaactt tatatgtgcg
     1501 gaattgctgg aatattttct tttgataaag ctagtactga atattcaaaa tatttagaaa
     1561 aaagtattag tgctttaata aagaataaaa atgaaaaacc aaatttattt attgaagaca
     1621 atattgcttt tgcctattta aaaaacacac aacacgctga aaatcaaaac aatattgtaa
     1681 taagcgatga taaaaactac ctcttattac ttgaaggcga tattttttat accgaaaatt
     1741 taaaaaatct aataaataat actgataaaa ttaaattaaa aactgactcc gaaattttac
     1801 tttttctttt aatagaacat ggcgaatcga ttattgaaaa actaaatgga aactttgtat
     1861 ttgcattttt taataaaata acaaacactc ttattgttag cagagactac ataggtttta
     1921 aaaatttgtt cttttataaa gatgatgaaa aatttatttt ttcaacagaa ttaaaatcta
     1981 ttttacaatt tgatattaca aaagaaatta attctgccgc attattctca tacctacaat
     2041 tgaactatat tttaccaaat gtaagtatca ttaaaaatat aaataaactt ggatacggca
     2101 gttttttaaa aattaataaa caaggatttg aaattaaaaa ctattataaa ataataccaa
     2161 gttttaatcc tgaaaattat gacaattttg aaatagcttc ttctaagtta agacaattgt
     2221 taagtgatgc aattgaagac aacttagcta aacaccctga tgcttcttgc ttcttatcgg
     2281 gtggaattga ctctagctta ataacagctt tagcctcaaa gtttaaaaag aatttgaata
     2341 ctttttctat tggatataaa gatgtagatt tttttgatga aacccaattt gctgaattag
     2401 ttgcacaaaa atataatacc aatcatacta gttttaaaat tacaaaccaa gaattgttag
     2461 ataatattga ctccgctttt gataattttg acgaaccgtt tgccgatagt tctgctcttg
     2521 ctttaaatat tttaagtaaa catgttagtc aacatagctc tgtagtttta agtggagatg
     2581 gagctgacga gatgtttgca ggctacaaca agcattctgc acattttcaa gctagctatc
     2641 ctggtacaaa agaaaaaatt gcagctgggt taaatccaat ttggaaaata gttccaaaat
     2701 ctagaagctc aaaaatgtca aatttagcac gacaattaga taggtttgcc actggatacg
     2761 ggcttaatcc taacgacaga tattggtttt gggcaactat tggaacagaa gaatacgcaa
     2821 aaaatatttt aaaattacca attgattact ctcagttttc agcaaaaaaa cacaaatata
     2881 taaatcaaga tatttgttta aaagatataa ataacgtatt actaaccgac atgaatttag
     2941 ttttgcaagg cgatatgctt atcaaagtta gcacaatgtc ggcaggaaat agattaaaaa
     3001 tatttgcacc ttttttggat aaaaacatta ttgattattc gtttagttta ccatcacatt
     3061 ttaaaattaa ttccacaagt cgaaaacgaa ttttaaaaaa tgcatttaac gatttgcttc
     3121 ctgatgaaat tttcaatcgt cctaaacatg gttttgaagt tcctttgcta atgtggttta
     3181 aaaaagattt aaaacatcta attgaggata aaattttatc tgaagatttt ataaaagagc
     3241 aaggaatttt taattacaaa gaaatttcaa gtctcaagca aaaactcaat tctccaaacc
     3301 cacacgatag tcctgcaaaa atatgggcaa ttatagtttt tcaaaaatgg tggacaaaac
     3361 atatgtaagt tatggcaaaa gttttaagaa ttattaatcg ctttaattta ggaggaccaa
     3421 catacaatgt tgcttacctc actaaattct tagcacctga ctatgacact ctattggttg
     3481 gagggtcaaa cactaaagac gaagcttgtt ctgattatat catcaaaaaa cttggtatcc
     3541 atgcaattac tatccctgaa atgagcagag aaattggata taatgattta atagcatatg
     3601 caaaaattaa aaacataata aaacgttaca aacccgatat cgttcatact catgcatcaa
     3661 aagctggata tttaggaaga agagctgcca taagtttaaa agtaccagtt atagtgcata
     3721 catttcatgg acacatattc cactcatact ttaataaaca agcaacatct ttatttaaaa
     3781 gcttggaaca aagtttagca aaaaaaactg atgctataat agccattagt cctatccaaa
     3841 aagatgaatt atgtaatatt cataaaattg ctccagaaga aaagttcaga ataattccat
     3901 taggatttga tttaaataga tttcaagaaa atttacagga aaaaagaaaa atatttagaa
     3961 caagatataa cttaaaagat gatgacattg taatttcaag tgttggacga ttagtgccaa
     4021 taaaaaacca cccattattt ataaaatcta ttgctaaagt taaagaaaaa acaaataaga
     4081 acgttaaagc tatgattgtt ggagacggct ctcttcgcaa tgaatttgtt gagctagcac
     4141 attctttaaa tttgaatgtt tctacacccg aaaaaacaga accaaatccc gacatcattt
     4201 tcacatcttg gatagaagat gcagacatag tttttgcagg ttcggaaatt gcagcattaa
     4261 gttcatttaa cgaaggcact cctgtatcat taattgaagc tcaagcatct agcatcccag
     4321 ttatatcaac agacgtaggt ggagttaaag atattataat agaaggtaaa acaggtttac
     4381 tttgtaaaaa tgatgatgtt gatgattatg ccgaaaaact tttaatttta attgaaaacg
     4441 acaagctaag agaagaaatg tcattaaagg gctgggaatt tgtaaaaaat aaatttcact
     4501 attccagatt agtaaatgat gttaaattac tctatgacga acttctaaaa aactcacaaa
     4561 taaaataaaa tacataaaaa atcatatcgc attttaagat tcaattaatt taattaattt
     4621 tgtgaaccaa aattttaaaa ttttacttga tgtttacttt attttttaaa gaaataaaag
     4681 agttttttag ctcagtaaca ggataccttg ttgtgataat ttttttgcta ataaactcaa
     4741 tgtttttatg gatatttaaa ggagttttca atatttttga cctcgaaatg gcaaatattg
     4801 atagcctttt tataatttca ccttgggtat ttttattttt agttccagca attactatga
     4861 atatgtttgc aggagaatac aagtctggaa ctatggaatt acttttaacg cgtccattaa
     4921 cagaatttca aattgttttt gccaaatact tggctagtgt tgtgcttgta ttagtggctt
     4981 tagctcctac tctactatat ttattttcaa tttatttttt agcagatcct gtcggcaacg
     5041 ttgatttcgg tgctattttt ggttcttata taggattgtt tttcttagca gctatttatt
     5101 ctgcattagg aatatttgca agttcattaa caaaaaatat tataatatct tttttactcg
     5161 gattattatt ttgttatgta ttttttcttg gttttgaaaa tctttcatat ttaataagtt
     5221 cgggaaaact ctcaaatttt attatttcac tcggaataaa cgaccactat aggtctatga
     5281 gtcgtggagt aattgatagt cgcgatgtcg tttatttttt atcggtaatt agcatattta
     5341 tttttgttac aaaacttaga ttagaaagtc gtaaatggta aatagtatgg aaaacaataa
     5401 taaaaaaaat aaaacctctc gctcgacatt aaaaaaacaa aatatacttc aagcgatatt
     5461 aattttggta ttaatcattg tcttcaacct gttgtcgtca atggtatttt ttaggcttga
     5521 cctcactaaa gaaaaaagat tttcgattac tgacacttca aaaaaaatat tagatgattt
     5581 agaagacaaa ataattattg atgtttattt agaaggagat gatttgcctg ttgaattgct
     5641 aagataccaa aaaactgtta aagaattttt agacaatttt aaatcatatt caaataaaat
     5701 agaagttatt tttaaaaacc cattcgaaga aggcaattct gaacaaaacc gagaaattta
     5761 ttttcaatta tataaaaaag gactaagccc aacttacatt agccaatcac aagatatggg
     5821 gcgaagtgaa aaaatgattt ttgctggagc acttttgaaa tatggagaaa aagaatttcc
     5881 aataaattta ataaacaaca atttaagttc tggaagcaat tatgtaggtt tgtccgaaac
     5941 tgaattagaa agagatttta tccatgctat ttggatgtta acaaacgaca aactcaaaaa
     6001 aattgctttt ctagatgggc ataaagagct ttcagaatat caaacttacg acataatgac
     6061 ctctctttca aaatattacc acatcgacag agtaattatg aatcaagttt taaatgcttt
     6121 agacggttat gatgtagtgg tggttgctaa gccagaagga tattttacag aaagagataa
     6181 gtttatcttg gatcaataca taatgcaagg tggtaaaatg atatggttag tagaatggat
     6241 gaaaattgat atggattctc taagtactaa accaatgcaa atggcaatgc taaacgatat
     6301 aaacttagac gaccaattat ttaattatgg tgttagaatc aaccctgatt taattcaaga
     6361 tttatcttgt ttaagtattc ctgttaatac agttgacgga aaaccacaat ttgagtctag
     6421 accttggccc ttttttccag ttattgtacc cgacacatta ttaaatcatc aattattaaa
     6481 aaacttagat atcatacgca cacattttgt tagctccata gatactgttg gagaaaatcc
     6541 aaaaattaag aaaaccattc ttttaagaac ttctaaattt tctaaaacca tgactcaccc
     6601 tgtagaggta aatttagaaa taatgagaaa cgaaccagaa ttaaaaactt ttaacaaacc
     6661 aaacttgcca atagctgttc ttttggaagg gcaatttaga tctaacttcg taaatcgctt
     6721 atcagatgaa tttgcaaaca atccagattt taaatttatt ccagaaagtg aagaaactaa
     6781 gatgatagta atttcagacg gagattttat tagaaacgaa gttagagtaa tgggaaacaa
     6841 acaacaaccc tatcccttgg gtgctgacaa atattatccc gaacaattta cgccaggaaa
     6901 tacccagttt tttgtaaact gtgttaatta tttatgtgct gacgatgatt ttatatcctt
     6961 acgtatgagg gaaatcaaaa ttagaaaact taataaagcc gaacttcgag aaaataaaca
     7021 acactggctt tattttaact taacacttcc tattttgtct attattataa taggtgttgt
     7081 tattcttttt tctagaaaaa ttaaatttaa caaaaaacct ttaaaaattc ataaaactta
     7141 ttctaaaaaa tgaaaaaaac aaatttaata ttactcataa tagttttagt tttagttata
     7201 gttactgcaa gtatatattt ttttcagaac acaagtagtc aacctgctta cagagatttt
     7261 gcaattaaag atactgcaag cattgataaa atttttattg ccgacaaaaa cagcaatact
     7321 gttttattag aaagaaaaga tggctattgg gaagcaaata aaagttttgt agcaagaaga
     7381 gatttgacaa acctattgtt agaaactttt tacagaattg aagttagttc gcctgttcct
     7441 aattcaaaat tagacaaagt tttaaaagac ttatcagtta gtggtgttaa atgtgaaata
     7501 tatcaaaatg gaaaactttc aaaaacttac tatataggtg gagttacaga tgacaacatt
     7561 ggctcataca tgattatgga aggctctgac cagccgttta ttgtgagaat tcctggattt
     7621 aacggtttct taaatgtaag atataatact aacccaaacg aatggaaaga aaaacttatt
     7681 tttaattaca aagtacagga cattgccaaa gttacagtag aatattcaaa ttcgcctgat
     7741 gaatctttta ttgcaattag taaaggtaat aatgaatttg atttaaaaga aataaatgga
     7801 agtaaagtta aattcgattt cgacacaata aaagtaaaaa cttatatttc atcatgcaaa
     7861 ctaattggct tcgatgctta tatttctgat tccttacaac aagaaaaata cgacagcctt
     7921 tcggttcttc catttgtggc aaaatacacc attgaagaca ttcatggaaa cgcaaaaagt
     7981 tttaaaacat atctaagaca aaatataaca aaacttttag atgacgaagg aaacttatac
     8041 gaatgggaca tagattattt atatggaatc cttgatgata aaactgttgt tttattacaa
     8101 tattatgtta tagaccccat agcaacaggc aaatacgagt ttattaaaaa cacagaagaa
     8161 catgattatt aaaatactta acaaagattg ttcataaaaa acttagtatt ataagtaaaa
     8221 atataataaa atatattaaa tttaaccaca aattaaaatt gaatgaatat gaattttgta
     8281 gtatcaagcg ccaacctttt aggacaattg caattagtaa gcaaagttgt tgttagcaaa
     8341 aacgctcttc caattttgga taatttactt tttgatatta aagacggtat attaacagtt
     8401 acaggtagtg acttagagac taccattcaa actaaaatag aactcacttc atgcgaagga
     8461 gaaggtagta ttgctataga cactaaaaaa atattagaaa ttttaaaaga attgccagac
     8521 caaccattaa ctttccaaat taatgttgaa gaacaaaaaa tagacattac ttcttcaaat
     8581 gggcaattct ctatgactgg tcaattaggt gatgattttc ctgtaattcc ttctattaaa
     8641 tcggaaaaag aaaagaagtt tgacataatg ccagacattt tatacggtgg aatatcttct
     8701 actatttttg caactggaga tgacgaacta agaccaatta tgaatggaat tcttattgaa
     8761 tttaaaaatg ataaagtaag tttcgttgca acagatgctc ataagttagt aaaatactct
     8821 cgtaaagaat taaaaacaga tgaagaagta tcttttatat tgcccaaaaa accagcaaat
     8881 cttataaaag gaattttacc taaagttttt tctgatgtcg aactaacttt tgatgacaaa
     8941 aatgctttat tcacttttga aaacacaaaa ctaatttgcc gcttaattga aggaacttat
     9001 ccaaactatg ctacagttat tcctaaagat aatccaaaca aattaattgt ggatagatta
     9061 gaattatttt cttctctaaa aagagtttcg atttttgcta gccaatcaag tagtttagtt
     9121 ggcttagaat ttactggcaa cactttagaa attagtgcta aagacacaga ctattctgtt
     9181 gcaggtaacg aaactataaa ttgtcaatat tttggtgaca atatgaaaat tggttttaaa
     9241 tctaacttct taatggaaat acttagcaac atttcaactc aagaagtaat tttcgaatta
     9301 tcagacccaa cacgtgcagg tataatttta cctaaagata acgaaaatga aaacgaagat
     9361 gttttaatga tattaatgcc tatgatgcta aacaattaat tttcataacc ataaataatc
     9421 aggggcttta tgcccctttt ttatctcact atatatttta tactatgaat attaaactaa
     9481 aaaaaccgat tgtatttttc gatctcgaaa ccacaggcat taatccagca acagatagaa
     9541 ttgttgaaat ttctatttta aaagtttttc caaacggaaa tgaaactatt aaaacttttt
     9601 taataaaccc agaaataaac ataccaagcg aagctagtgc aatacacgga atcactaacg
     9661 atgacgttaa agatgctcct acttttaaaa tgttagcaaa agaaattgta agcttaatgg
     9721 acgattccga ccttgctggt tacaattcta ataaatttga catacctctt ttagcagaag
     9781 aattattaag agcaggttct gattttgacc taaaaaagcg aaattttatt gacgtaatgg
     9841 taatttttat gaaaaaagaa cctagaaatt tagaagctgc ctacaaattt tattgcaata
     9901 aagaactcga aaatgctcac tcagctgaag ccgatataaa agcaacttat gaagttttta
     9961 aagcacaaac ccaccattac gacgatctaa atactgatgt agaaagcatt tcaaaatatt
    10021 catctcaaaa taaatatgtt gactttgccg gacgattaat acgcgatgaa aacgacaatg
    10081 ttattataaa ttttggaaaa cataagggaa aaaacataaa tcatgttttt gaaaaagata
    10141 ctgcttatta cgattggatt caaaaaaatg acttcccact ttatacaaaa aaaatgttta
    10201 cagaattgta cattaaattt aaacaaaaca aataaaatat taaacttagc tcatatgcaa
    10261 cgttacatta gtgttttact cctaattttt ataacaaatc taagttttgc tcaaaatcac
    10321 caaaccccag attggttgca agacttgcct tataacacaa gtgaatatat ctatgctatt
    10381 ggttttagcg atccaaaaat gagcgacagt atcttagcaa aaaacttagc tttaaacaga
    10441 gctttggcaa ttgctgttat ggtaaaccaa tcacaagttt cgtatgcctc agatttttat
    10501 gaagcaaaat cagaagaaca cagatggtat attacaaaag aatcatttga agaatttgga
    10561 aaaattaaag caagtgcata tgttaataat aattacgaaa ttgtaaaatc agaaactaat
    10621 agtaacggtg agttaataat actattaaag tttttcccct ctcaagaaaa aggtgtagaa
    10681 cataatttta atatagaaat tgaatattat agtcaagttt ttgaattaag taatacaaga
    10741 gcacttgaac attttaggaa tataatgtta aatacttgtt ggacaaactt aaaaacaaaa
    10801 gatagcctta aaacttatta cgaactttct aattacaata attacataaa tataaaagaa
    10861 tcctatggca cactagaaat agaggctcct agatttcatt ataattacaa acctagttta
    10921 gcaaataaaa ttgattttaa agattttagt tcttactctc ctttgcaaaa aggactttgg
    10981 caatcatatt tagactcata cactcaaagt atatttaaca ttaataaaaa ccattcctcc
    11041 aaaataggca ctatccaaga cgaatttaaa agaagcaaaa acacatatga ttctcaacaa
    11101 gggcaagggc aaaaaacaga aagtttaact agaaacactt taaggaatac tctaaatttt
    11161 gaacttggcg ggatagaaat atacaacaac tatttatata acagagtgta tttacaaaat
    11221 aaaagaccat catatagtaa tgtaaattct tcccaaatta aaaacgaaaa tagcaacaat
    11281 gcaaaagata aaaaatcaaa agaatggttt tttaaaagat ggtttaagaa aaaatcataa
    11341 gtctccgccc cattaaacaa aaataataac aactgtgata aattaatatc aaagtttaga
    11401 agcaatatct tttagttaga tattttctaa actataatta aaaacaaata atctaaatta
    11461 atttaacact taattttctt cctctgtttc taaatcttgt tcttcaaaat attcgggttt
    11521 ctgttcttta atataactca aagcctgctc atatccttct ataaatttac caaaatcttc
    11581 cttatataaa aagattttat gtttctgaaa atgagagttc ccgtcctgtt ttccgcattg
    11641 ggacacccaa aaaaataatt cattaataat tttataa
//
LOCUS       DAOPNX010000083        11620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_6399_length_11620_cov_5.920104, whole genome shotgun sequence.
ACCESSION   DAOPNX010000083 DAOPNX010000000
VERSION     DAOPNX010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11620
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6399_length_11620_cov_5.920104"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..1892)
                     /locus_tag="PKN53_08850"
     CDS             complement(6..1892)
                     /locus_tag="PKN53_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOD89095.1"
                     /translation="MMKFILLLIGTVFVFTSFANDYKKYLIPEPKKITDNFQTSETSA
                     VNIIRLSTLFEAYKDKDFVGELPLVKYYHGISEIKFEIDENLKNNQEYKIEISETSIN
                     LSGADMASLFYAKQTFLQLLEYCKYENKALPCIVISDYPDFERRGFMLDVSRNKVPTM
                     ESLYYIIDLLASWKINELQLYTEHTFAYKNHKKVWEKYSPLTAEEIQELDKYCQQRYI
                     DLVPNQNSFGHLEYWLKYDEYVDLADCPEPCQTIWGPSQKTSLDPTNPKSFKLISELY
                     NELLPNFSSKYFNIGCDETMELGLGRSKTVCDKEGKGKVYLDYLKKLNAEVNKLGKTT
                     QFWGDIIINHSELIPKLPKNMIALVWGYEDSYSSGNHLPKFVNAGIDFYVCPGTSCWN
                     SLIGRNKNGFENLKNAALKGKAHNAKGYLITNWGDYGHWQPLSISMPAIMLGSAYAWN
                     YSKKSLENLEFLLNHYVFEDSTQNTAKAILKLGYVDQKTTVPNGNANIFHLMYYLYKR
                     NIDKHYQAKYMKANELIVCEKEILDALEILKLAKPSTKDSTIIIQEIELAAKLAIHAT
                     HLGVARLEADEKKIENIPAVKRKELYDELKSLIETHKKLWIVRNREGGLHDSAGKMEN
                     LLKK"
     gene            complement(2013..2405)
                     /locus_tag="PKN53_08855"
     CDS             complement(2013..2405)
                     /locus_tag="PKN53_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89096.1"
                     /translation="MNTNKPKVIQDFEKLSKEIQEQIKLTYPNGFSQHLIQFTNKEGR
                     FVSALPFETDDKYYLVRMTLDEAREIIYSDDDYDDDGILKNDVKEEFVDKYAELDYMS
                     DVIGVDDDDDFEEKYDEPYEDEDDDDED"
     gene            2816..5431
                     /locus_tag="PKN53_08860"
     CDS             2816..5431
                     /locus_tag="PKN53_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89097.1"
                     /translation="MYRIFLSLIIVFVALTGFSQNDSIDFDKFKKEYHNFIRQEKEAF
                     QRYKEERDKEFADFLKADWENYQLFIENKPIVIPGPDKIPNYNAALNASSSKRLKPKA
                     INEVKPTIVDESSYQLKPIPAIITSDTKKNYDMVNLDYYGNNIDFIYHKKLKEATINA
                     VSESQIANFWADACKSDYYNTIEQMLEYKNLNNLNDFAYMKLAEKISEKIHSNNKNNS
                     KLLTWFLLAKSGYKLKVGYSGNSVYILVPCVNVIYSYSYFVLENLKYYIFEEETQVKS
                     IYTYRQDYPEANKIMNFNIYKAPLLGPEKYIRELDFTYENKSYKFDIAYSKNLIEFYD
                     DYPQGEIQIFFNAGVSSFVKESLDQNIEPAIAEMTETEAANFLLNFVQTAFDYQTDQE
                     QFGYEKFFFPEEIFHYKYCDCEDRSVFYAYLVNEYLRLPVAGLNYPGHMATAVRFTET
                     VNGVYYLLDNERFVVCDPTYINAPIGACMPNYLKSEVTILRLKNSQVANSERNIIWQQ
                     LAQNGYIRTNYEYDIIKIDDNTWYVTGLVDSSTNFAGLKPKIIGDEEKLFFAKLDNKG
                     NILFLETISGDGLLMPIGIAHSKYGVYISGYFSKNIKAGDQKLEATYNKELFMACYDH
                     DHNLQWLRASGIVNEVETSNMFFAVNMDKKGNFIKTESISAESFENQKAIDLSNPDNI
                     TLFVKFDMPSPGLKDSELYNNPSSFEFATNLRDFTNDLLSQNYNKNTAPLLAFLKILQ
                     NGDISVTGIEILAAALSMNHDFKALAPQLFNSLKEIENISSKNGIINLNLKNPENFVL
                     GNLHFEKNSQFRVVNYKSGNIEIIVTKGISYKPHFKNYIVNFIKIFKASDDINIDYDF
                     DNDKKNLNIEKHLLK"
     gene            5562..6794
                     /locus_tag="PKN53_08865"
     CDS             5562..6794
                     /locus_tag="PKN53_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89098.1"
                     /translation="MEALGQYADAADMFYYAVQAKRTNVEALAGLKRCGQMTLSKKLS
                     EFNQAYNDSKNKEAVYFYQDAKAYYEKLNAVGVSLNFPSFYDEYYQEVKDTYLEDRYY
                     EGSKLLSDERFSEAETIFKEIQKLQANYKDSKDKLIVAVNEPKYREGLRLMEANQYRK
                     AYYIFDEIINNAGSYKSSYDLKSECLTKGTITIAIEKIKNQSGTSGIDAQLESKIINR
                     IQAANNPFIKLVDTKTSSAIKYPDARLYVEIPKFVYNRGQNKEEEKRGYLKKTIKVLN
                     KETDTYETKTEYSKVVYKEYAMSRSVDMNINFRLIDGKTSVIHTSSTKSSTASDNVHY
                     AKYDGDSKNLVPGYWKSRTSTNSEDIIKDSSREVSALQDLLKARTQIKDYNTLSNEAL
                     DECAAAVTNAVLRYVNNN"
     gene            6804..8192
                     /locus_tag="PKN53_08870"
     CDS             6804..8192
                     /locus_tag="PKN53_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HOD89099.1"
                     /translation="MKNKFIEMKLLQTIVLLMVFTLSVSCQTLKKQKFQKTPTPEWVK
                     VKPQHPSYYIGVSSSPKKGFTPADYMANAQQQALGDLASNISTNIESSSVLSVIETKY
                     HIGENYSKEITASTSHQLEGYELVETWEDENFYWAYYRLSKSHYAKIREEKKIQSIED
                     AKNKYYQATELMGRQLQYNAFQFYAEALASLKPYLGESTITEIYGEEKDLGNTIFSAL
                     ADFVNDINIVFKNNDISVKKGIKVDPDVFSFLVTDKNNNPISGIPVKIRFSGAGLLRN
                     SEVSSTDGKVFIAIDKIKSSPKIETLSINLDMVGLSRVSKDPVIKNLIKSLPSSEKQI
                     RVNVEKPVLNIQSEEKSFGQLRKENKLKSTFEGLLSSEFVVNPNLKSDFNMTITSNTT
                     KNGYNYGEAQVVINYTIDISDDRGNVVYRKSSSSEYSAADHNAADTKAYVELSKSIER
                     VIVREVISAVNQ"
     gene            8196..8504
                     /locus_tag="PKN53_08875"
     CDS             8196..8504
                     /locus_tag="PKN53_08875"
                     /inference="COORDINATES: protein motif:HMM:NF014022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HOD89100.1"
                     /translation="MSVLMSFAIFPTDKGTSVSEYVAKVVKMVSNSGFEYKLTAMTTV
                     VETENFEQALEILNKSYKILEPISDRVYCSVNFDIQTNKPNGRIKTKINSVIDKLDKE
                     "
     gene            8524..10056
                     /locus_tag="PKN53_08880"
     CDS             8524..10056
                     /locus_tag="PKN53_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89101.1"
                     /translation="MKNIFVFIFLFFFSFCSLAQNYPLTEHQQNINPNLIYSENNFTL
                     QKPFTGIENKTVDSLIFTQTTSKYKNWFLRKIFDENLFVKQDDKFIVILNPLLDFRLN
                     YQNNTRGYHNTRGVMLSGKLDSKIYFNSCFTESQAIFPENIKKYFDEFGVIPGYGRIK
                     PLSRSGEFDFGAAYGNIAVKACKFLDISLGYDKLFIGDGYRSLILSDFSAPMMYAKTA
                     FNYKKFQINNIFTKAINPNFNNVLQHENPVSLNSRYPAKHVSFNTLTYKPTKAWQISL
                     IEALVLSQNIDEWNYPAYILLPFVRTTFINYKKQLTNNFVGVNTTWQNKKIGIIYSQI
                     FIDKISKSDLIASLQIGYKNFDFLKVKNLFFQIEYNRVPREMYLHANNDLHYGHYNQA
                     IAHPAGAGFNEILAIADYKFKRLEVLTKSVFLKYATINSEYNQQNIFDYSLLYNVDAN
                     TFTKNPMLISDVQIIYNLNNSYRLQIFAGISNRADLTTKNSTNFIQCGLRTALRINYD
                     DF"
     gene            10057..>11620
                     /locus_tag="PKN53_08885"
     CDS             10057..>11620
                     /locus_tag="PKN53_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89102.1"
                     /translation="MKKIVLNIITLFLVFAGFSQNLPILDPFSIRNYEKESLVDNKFH
                     SSIKSYYFSDFQNSILDREKALVFYKHNFKNAKEKTQFYISPEFDIANFLAPSDNKVY
                     GINPSLGALLEFSYADKLGISYNFNAWYISKMPSYTFKTLGNSTIYHGLGKNILENQK
                     LFLENNLKITYSPYDFLRLELGNAKNFYGDGYRSLLLSDFASNYPYLKLETSFLTVKY
                     SCIWAVHQDLGRIIPENGNLKTENKFDVFHYLDWKIGNHVNLGLFEAIITSKQKFFNF
                     EYLNPIIFFRPVEFSLGSEDNALLGLNLKVKINKNNFIYSQFVLDDIIVTQLINDIKH
                     SVNPNYTGEYGWFANKWATQFGFKSYDIFKLKNLDFFTEINIARPYIYSHVHTNQNYS
                     HLWQALAHPLGANFVESVTGLTYYGERFVIDTKLMFAKIGKDSVNSHFGSNIFLATMD
                     GAQPYPYRVNSYGNVILQGKSTNNISFIVDFAYSLLKDKNLFANISLIYRAADYNLDS
                     SKNEFYICFGIKS"
BASE COUNT         4273 a         1614 c         1628 g         4105 t
ORIGIN      
        1 ttcttttact tttttaataa attttccatt tttcctgcag agtcatgcaa gccaccttct
       61 ctatttctta ctatccaaag ttttttatga gtttctataa gcgattttaa ttcatcgtat
      121 agctcttttc tttttacagc aggaatattt tcgatttttt tctcatcagc ctctaaccta
      181 gcaactccaa gatgagtagc atgaatagcc aattttgctg ctaattcaat ttcttgaata
      241 ataattgtag aatcttttgt gctaggttta gcaagtttta aaatttctaa ggcatcaaga
      301 atttcttttt cgcaaacaat aagttcgttt gctttcatat atttagcttg gtagtgctta
      361 tctatatttc gtttgtacag ataatacatt aaatgaaaaa tatttgcatt tccattagga
      421 actgttgttt tttggtcaac atatcctaat tttaaaattg ctttagcagt attttgagta
      481 gagtcttcaa aaacataatg atttaaaaga aattccaaat tttctaaaga ttttttggaa
      541 taattccaag cataagcaga acccaacata attgctggca tagaaattga caaaggttgc
      601 caatgcccat agtcgcccca atttgtaatt aaataacctt ttgcattatg tgcttttcct
      661 tttaaagctg cattttttaa attttcgaaa ccatttttat ttctacctat taatgaattc
      721 cagcacgatg ttcctggaca aacataaaaa tctatgccag cattaacaaa ttttggcaaa
      781 tgatttcctg atgaatagct gtcttcgtaa ccccagacta aagctatcat gttttttggc
      841 agttttggaa ttaactctga atgattaata ataatatctc cccaaaattg agtagttttt
      901 ccaagtttat ttacttcagc attaagtttt ttcaaataat ccaaatatac ttttcctttg
      961 ccttctttgt cgcaaactgt tttagacctt cccaaaccta attccatagt ttcgtcgcaa
     1021 cctatattaa aatatttgct tgaaaaattt ggtaaaagct cattatataa ttccgaaatt
     1081 agcttaaaag actttggatt agttgggtca agacttgttt tttgacttgg tccccaaatt
     1141 gtttgacaag gctctggaca atctgctaaa tcaacatatt catcatattt tagccaatat
     1201 tctaaatgtc caaaagaatt ttgatttgga acaaggtcaa tatatctttg ctggcaatat
     1261 ttatcaagtt cttgaatttc ttctgctgtt aaggggctat atttttccca aactttttta
     1321 tgatttttgt atgcaaaagt atgttctgta taaagttgca attcgttaat tttccaagat
     1381 gctagcaaat caattatata atataagctt tccattgttg gcactttatt tcgactaaca
     1441 tcaagcataa aacctcttcg ttcaaaatcg ggatagtctg aaattacaat gcaaggaagt
     1501 gctttatttt catatttgca atactctaaa agttgtaaaa aagtttgttt tgcataaaac
     1561 aaagaagcca tatctgctcc tgataaattt attgaagttt cgctaatttc tattttatac
     1621 tcttgattat tcttcaagtt ttcatcaatt tcaaatttta tttcagatat accatgataa
     1681 tattttacaa gtggtaattc gccaacaaaa tctttatcct tatatgcttc aaaaagtgtt
     1741 gacaacctaa taatattaac agcagaagtc tctgaagttt gaaaattatc tgttattttt
     1801 ttgggttctg gtattaaata ttttttataa tcgtttgcaa aagaagtaaa tacaaacaca
     1861 gttccgatta agagaagtat aaatttcatc attttataaa ttaatttaga attaagaatt
     1921 ttggattttg gattttggat ttttgattta cgatttttga ttttagattt cggatttaat
     1981 tcttaattat caattcttca attatcaatt aattagtcct cgtcatcatc atcttcgtct
     2041 tcgtaaggct catcatattt ttcttcaaag tcgtcatcat catcaactcc tatgacatcg
     2101 ctcatataat ctagttcagc atatttgtca acaaattctt ccttaacatc attttttaaa
     2161 attccgtcat catcataatc gtcgtctgaa taaataattt ctctcgcttc gtccaatgtc
     2221 attcttacta gataatactt atcgtctgtt tcgaaaggca aagcacttac aaatctacct
     2281 tctttatttg taaattgaat taaatgttga ctaaatccat ttggataagt taattttatt
     2341 tgctcttgaa tttccttact caacttttca aaatcttgta ttactttcgg tttattggta
     2401 ttcatatctt aattttttta taactataat tatcaatatt aattttgtgt aaatgtgata
     2461 aattttttaa tacttcaaaa ataattgaaa aaaaaatata aatttttata cacaaacata
     2521 tctaactgat attcaggaac ttattttaag actttgaata tttttcaagc attttagaca
     2581 aatctgcact agttatttga ttttcgcaca tcaaaaaaca acagtttttc cacttaataa
     2641 ttttgtttaa gtaaaatttg attaacaaaa agcttttttc taggctaaca attattttaa
     2701 acttgtcttt taaaatttaa aaacttttct taattttaaa aaattataaa atatcattat
     2761 taattttact ttgtggcaca aaaaatgtac ttattaataa ataaaactca aaattatgta
     2821 tagaatattt ttatcactca tcattgtttt tgttgctcta acaggctttt ctcaaaatga
     2881 tagtattgat tttgacaaat ttaagaaaga atatcataat tttattcgac aagaaaaaga
     2941 ggcttttcag cgatataaag aagaacgcga taaagaattt gctgattttt taaaggctga
     3001 ttgggaaaat tatcaacttt ttattgaaaa taaacctatt gtaattcctg gtcctgataa
     3061 aattccaaat tataatgcag ctttgaatgc ttcttcttca aaaagattaa agccaaaagc
     3121 tataaatgaa gtaaaaccca ctattgttga tgaaagttct tatcaattaa aaccaattcc
     3181 tgcaataatt acttcagata ctaagaaaaa ttatgatatg gtaaatttgg actactacgg
     3241 taataacata gattttattt atcacaaaaa attaaaagaa gcaacaataa acgcagtatc
     3301 agaatcgcaa attgcgaact tttgggcaga tgcttgtaaa tcagactatt acaacactat
     3361 agaacaaatg cttgaataca aaaacttaaa taatttaaac gattttgcct atatgaaatt
     3421 ggctgaaaaa atttctgaaa aaattcacag taacaataaa aacaactcaa aactactaac
     3481 ttggttttta ttggcaaaat cgggatacaa actaaaagtt ggctatagtg gtaattccgt
     3541 atatatttta gttccttgtg ttaatgtaat ttattcttac tcatattttg tgcttgagaa
     3601 tttaaaatat tatatttttg aagaagaaac tcaagtaaaa tcaatttaca cttatcgtca
     3661 agactatcca gaagcaaaca aaattatgaa tttcaatata tacaaagctc ctttgttagg
     3721 tcctgaaaaa tatattagag aattagactt tacctatgaa aacaagtcgt acaaatttga
     3781 tattgcttac agtaaaaatc taattgagtt ttatgatgat tatccacaag gcgaaattca
     3841 aatatttttt aacgctggcg tatcaagttt tgtaaaagaa tcattagacc aaaatataga
     3901 gccagctatt gcagaaatga ctgaaacaga agctgctaat tttcttttaa attttgttca
     3961 aactgccttt gattatcaaa ccgaccaaga acaatttggc tatgaaaagt tcttttttcc
     4021 tgaagaaatt tttcattata aatactgcga ttgcgaagac agatctgttt tttatgctta
     4081 tttagtaaac gaatatttac gtttacctgt ggctggatta aattacccag gacatatggc
     4141 tacagctgtt agatttacag aaactgttaa cggagtgtac tatcttcttg ataatgaaag
     4201 atttgtagtt tgcgacccta cctatattaa tgctcctata ggtgcttgca tgcctaatta
     4261 tttaaaatct gaagttacaa tattaagatt aaaaaactca caagtagcta attcggagag
     4321 aaatataatt tggcaacagc tagcacaaaa tggttatata cgcacaaatt atgaatacga
     4381 cattataaaa attgatgata acacttggta tgttactggt ttagttgatt catcgacaaa
     4441 ttttgcagga ttaaaaccaa aaataattgg agacgaagaa aaattatttt ttgctaagct
     4501 tgataacaaa ggaaatatac tttttttaga aacaatttct ggtgatggat tgcttatgcc
     4561 tattggtatt gcacattcta aatatggagt ttatatttct ggttacttca gtaaaaacat
     4621 aaaagcaggc gaccaaaaac ttgaagcaac atataataaa gaattattta tggcatgtta
     4681 cgaccatgat cataatttgc aatggcttag agctagcgga atagtaaacg aagtagaaac
     4741 ttcaaatatg ttttttgcag tgaatatgga taaaaaagga aacttcataa aaactgaaag
     4801 tataagtgct gaaagttttg aaaatcaaaa ggctattgac cttagcaatc cagataatat
     4861 aacattattt gtaaaatttg atatgccaag tcctggactt aaagattctg aactttataa
     4921 taatccaagt tcatttgaat ttgctacaaa tttaagagat tttacaaatg atttgctttc
     4981 tcaaaattat aataaaaata ctgctccttt acttgctttt ttaaaaattt tacaaaatgg
     5041 agatatttct gttacaggaa ttgaaatttt agctgctgct ttgtctatga atcatgattt
     5101 taaagccctt gcacctcagt tatttaatag tttaaaagaa attgaaaata tttctagtaa
     5161 aaatggtata ataaatttga atttaaaaaa tccagaaaac tttgtgctag ggaatttaca
     5221 ttttgaaaag aactcacaat ttagagtcgt aaattataaa agtggaaata ttgaaattat
     5281 agtaaccaaa ggcattagct ataagccaca ttttaaaaac tatattgtaa atttcataaa
     5341 aatattcaaa gcaagcgatg atataaacat tgactatgat tttgacaatg acaaaaaaaa
     5401 tctaaacatt gaaaaacatt tattaaaata gtattatatt tgaacaaaat tttatttaac
     5461 atctcatgaa aaaaattatt agtattagtc taattttcct cagcctcata ttggttaaca
     5521 cgggttgtca aacgaaaatt tacacaaaaa aagctttaaa aatggaagct ttaggtcaat
     5581 atgctgatgc ggcagatatg ttttattatg ctgttcaagc aaaacgtaca aatgttgaag
     5641 ctttagcagg gcttaaacgt tgtggtcaaa tgactttaag taaaaaacta tcggaattta
     5701 atcaagccta taatgatagt aaaaataaag aggctgtgta tttttatcaa gatgcaaaag
     5761 catattatga aaaattaaat gcagttgggg tttcattaaa ttttccttca ttttatgatg
     5821 aatattacca agaagttaaa gacacatatt tagaagacag gtattatgaa ggttcaaaac
     5881 tgcttagcga tgaacgtttt tctgaagcgg aaactatttt taaagaaata caaaagttac
     5941 aagctaatta taaagactct aaggataaat taattgttgc agttaacgag cctaaatatc
     6001 gagaaggttt acgtcttatg gaggctaacc aatatagaaa ggcttattat atttttgatg
     6061 aaataataaa taatgctggt tcttataaaa gttcttatga tttaaaatca gaatgtttaa
     6121 caaaaggcac aatcactata gccattgaaa aaataaaaaa tcaatctggc acttcgggaa
     6181 ttgatgctca attagaaagc aaaataatta atagaataca ggctgcaaac aatccattta
     6241 taaaattagt tgatacaaaa actagctcgg caataaaata tccagatgct agactttatg
     6301 ttgaaattcc aaaatttgtt tataacagag gacaaaataa agaagaagaa aaaagaggtt
     6361 atttaaagaa aactataaaa gttttgaata aagaaactga tacttatgaa actaaaactg
     6421 aatatagtaa agttgtttat aaggaatatg ctatgtcacg ctctgttgat atgaacataa
     6481 atttcagatt aattgatgga aaaacaagtg ttatacatac tagttctact aaatccagca
     6541 cagcttctga taatgttcat tatgctaaat atgatggaga ttctaaaaat ttagttccag
     6601 gttattggaa gagtagaact tcaacaaatt ccgaagatat tataaaagat agttcaagag
     6661 aagttagtgc attgcaagat cttttaaaag caagaacaca aattaaagat tataatactt
     6721 tatcaaatga agcacttgat gaatgtgcag ctgctgttac caatgcagtt cttagatatg
     6781 ttaataataa ttaaatgcta ttaatgaaaa ataaatttat tgaaatgaaa ttattacaaa
     6841 ctatagtatt attgatggta tttacgctta gtgtttcgtg tcaaacatta aaaaagcaaa
     6901 aatttcaaaa gacccccact cctgaatggg taaaagtcaa acctcagcat ccttcatatt
     6961 atataggtgt aagttcttct ccaaaaaaag gttttactcc cgctgattat atggctaatg
     7021 cacaacaaca agctctaggc gacttagctt ctaatatctc aacaaatatt gaaagttcat
     7081 cagtattatc tgttattgaa actaaatatc atattggaga aaactattct aaagaaataa
     7141 cagcttcaac tagccaccaa ttagaaggtt atgagcttgt tgaaacttgg gaagatgaga
     7201 atttttattg ggcttattat cgtttgtcaa aatctcatta cgctaaaatt agagaagaga
     7261 aaaaaataca aagtattgaa gatgctaaaa ataaatacta tcaagctaca gaacttatgg
     7321 gtcgtcaact tcaatacaat gcttttcaat tttatgccga agcacttgct agtttaaaac
     7381 cttatcttgg tgaaagtact attacagaaa tatatggaga agaaaaagat ttaggaaata
     7441 ctattttttc tgctttagct gattttgtta acgacattaa tatagtcttt aaaaataatg
     7501 acataagtgt taaaaaaggc attaaagtag atcccgatgt ttttagtttt ttagttacag
     7561 ataaaaataa taatcctata tctggaatac ctgtaaaaat tagattttct ggtgcaggat
     7621 tattgcgtaa ttcagaagtt tcaagcactg acggtaaagt atttattgct attgataaaa
     7681 ttaaatctag tccaaaaatt gaaactttgt cgataaatct tgatatggtt ggactttcga
     7741 gagtatcaaa agatccggta attaaaaatt taattaaaag tcttccttca agcgaaaaac
     7801 aaataagagt aaatgtagaa aagcctgttc taaatattca gtccgaagaa aaatcttttg
     7861 gacaattacg caaggaaaat aaattaaaat caacttttga agggctatta agtagcgagt
     7921 ttgttgtgaa tcctaactta aaaagtgatt ttaatatgac aattactagt aacactacaa
     7981 aaaatggtta taattatggc gaagcacaag tagtaataaa ttataccatt gatatttctg
     8041 acgacagagg taatgttgtt tatagaaaat ctagttcttc ggaatattct gctgccgacc
     8101 ataatgctgc tgatactaaa gcttatgtag aactcagcaa atctattgaa agagttattg
     8161 taagagaagt tataagtgct gtaaatcaat agattatgtc ggttttaatg agttttgcaa
     8221 tatttccaac tgataaagga actagtgtta gcgaatatgt agcaaaagtt gtaaaaatgg
     8281 tttcaaactc aggatttgag tacaaactta ctgcaatgac tactgttgtt gaaaccgaaa
     8341 attttgaaca agcattagag attttaaata aatcatacaa aattttagaa ccaatttcgg
     8401 atagagttta ctgctctgta aattttgata ttcaaaccaa caagcctaat ggcagaataa
     8461 agacaaaaat aaattcggtg attgataaat tagacaaaga ataaaaattt gtgcctttat
     8521 aaaatgaaaa atatatttgt tttcatattt ctattttttt tcagtttttg ctctttggca
     8581 cagaattatc ctttaaccga gcatcaacaa aacataaatc caaacttaat ttattccgaa
     8641 aataatttta ctttacaaaa accatttact ggaatagaaa acaaaactgt tgacagttta
     8701 atttttacac aaactacaag caaatataaa aattggtttt taagaaaaat ttttgacgaa
     8761 aatctttttg tgaaacaaga cgataaattt attgtaattt taaatcccct actagacttt
     8821 agacttaatt accaaaacaa tacgcgaggc tatcacaata ctcgtggtgt tatgcttagt
     8881 ggaaagttag attcaaaaat ttattttaat tcttgtttca cagaatctca agctattttt
     8941 cctgaaaata ttaaaaaata tttcgatgaa tttggagtta ttcccggata tggaaggata
     9001 aaacctcttt ctcgttcagg cgaatttgat tttggtgctg cttatggaaa tattgctgtt
     9061 aaagcttgta agtttttgga tattagttta ggctatgata aactttttat tggcgatggc
     9121 tatcgttctc tgatattatc tgatttttct gcaccaatga tgtatgcaaa aactgcattt
     9181 aattataaaa aatttcaaat caataatatt tttactaaag ctataaatcc taatttcaat
     9241 aatgtattac agcatgaaaa tcctgtaagt ctgaactcta gatatcctgc taaacacgta
     9301 tctttcaata ctttaacata caagccaact aaagcatggc aaatttcatt aattgaagct
     9361 ttagttttat cgcaaaatat tgatgagtgg aattatcctg cttacattct cttgcctttt
     9421 gttagaacta catttataaa ttataaaaaa cagcttacaa ataattttgt tggtgtaaat
     9481 acaacttggc aaaacaaaaa aattggaata atatattcac aaatatttat agacaaaatt
     9541 tcaaaatctg acttaatcgc ttcattacaa ataggttaca aaaattttga ttttttaaaa
     9601 gttaaaaact tatttttcca aattgaatat aatagagttc cacgcgaaat gtatttacac
     9661 gcaaacaatg acttgcatta tggacattac aatcaagcta ttgcccaccc tgctggtgct
     9721 gggtttaatg aaattttagc tattgctgat tacaagttta aaagactaga agttttaaca
     9781 aaatctgttt tcttaaaata tgctacaatc aattcagaat ataaccaaca aaatattttt
     9841 gattattcac ttttatataa tgttgatgca aacacattta caaaaaatcc aatgctaatt
     9901 tctgatgtcc aaataattta caatttgaat aattcttata gactacaaat ttttgcagga
     9961 atttcaaatc gagcagattt aacaacaaaa aatagtacta atttcattca atgtggatta
    10021 agaacagctc taagaataaa ctatgatgat ttttaaatga aaaaaattgt tttaaatatt
    10081 attactttgt tccttgtatt tgcaggcttt tcgcaaaact tgccaatact agatccattc
    10141 tcaattcgca attatgaaaa ggaaagttta gtcgataata aatttcattc ttcaataaaa
    10201 tcatattatt tttctgattt tcaaaactca atattagata gagaaaaagc acttgtgttc
    10261 tataaacata attttaaaaa tgctaaagaa aaaacacaat tttatataag tccagaattt
    10321 gatattgcga attttcttgc tccaagtgat aataaagttt atggaataaa tccaagttta
    10381 ggtgctttgc ttgaattttc ttatgctgac aaattgggaa tttcctataa ttttaatgct
    10441 tggtatatta gcaaaatgcc aagttatact ttcaaaactc ttgggaatag tacaatttat
    10501 catggtttag gaaaaaatat tttagaaaat caaaaattat ttcttgagaa taatttaaaa
    10561 ataacatatt ctccttacga ttttttaaga ttagaattag gaaatgctaa gaatttttat
    10621 ggtgatggtt atcgctcact tttattatct gattttgcgt caaattatcc atatttaaaa
    10681 ttagaaactt catttttaac agtaaaatat tcctgcattt gggctgtaca ccaagatttg
    10741 ggtagaatta ttcctgaaaa tggcaattta aaaactgaaa ataaatttga tgtattccat
    10801 tacttagact ggaaaattgg aaatcatgta aatcttggat tatttgaagc gataataact
    10861 tctaaacaaa agtttttcaa ttttgaatat ttgaatccta taatattttt ccgtcctgta
    10921 gagttttcat taggatcaga agataatgca ttattaggtt tgaatttgaa agttaaaatt
    10981 aacaaaaata attttattta ttctcagttt gtacttgacg atattattgt tactcaacta
    11041 attaacgaca taaaacatag tgtaaatcct aattatactg gagaatatgg ctggtttgca
    11101 aataaatggg caactcagtt tggttttaaa tcttatgata tttttaaatt aaaaaacttg
    11161 gattttttca ctgaaataaa tattgctcga ccatatattt attctcatgt acatacaaat
    11221 caaaattatt cacacttgtg gcaagcatta gcacatcctt tgggtgcaaa ttttgttgaa
    11281 tctgttacag gacttactta ttatggcgaa agatttgtta ttgacacaaa attaatgttt
    11341 gctaaaattg gaaaagactc tgtaaactca cactttggaa gcaatatttt ccttgcaaca
    11401 atggacggtg ctcagcctta tccttacaga gtaaactctt atggaaacgt aatattgcaa
    11461 ggaaaatcaa ctaacaacat aagttttatt gttgattttg catactcttt attaaaagac
    11521 aaaaatttat ttgcaaatat ttcactaatc tatagagctg cggattacaa cttagacagt
    11581 tcaaagaatg aattttacat ttgttttgga ataaaatcga
//
LOCUS       DAOPNX010000084        11309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_6595_length_11309_cov_7.177981, whole genome shotgun sequence.
ACCESSION   DAOPNX010000084 DAOPNX010000000
VERSION     DAOPNX010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11309
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6595_length_11309_cov_7.177981"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(293..1621)
                     /gene="miaB"
                     /locus_tag="PKN53_08890"
     CDS             complement(293..1621)
                     /gene="miaB"
                     /locus_tag="PKN53_08890"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOD89103.1"
                     /translation="MNKKIYIETYGCQMNVADSETVAGIMIAKNYFGVNKINDADLII
                     INTCSVRDNAEQKIWNRLDHIKALKKKNKNLKVGIIGCMAQRIGEKLLEHDAVDFVAG
                     PDAYRSLPELIDDNNDCLKQKNLILDINETYSGVEQIKQNQDEISGFVPITRGCNNFC
                     SYCIVPYTRGRERSSSYIDIINEVKNLESKNFKEVTLLGQNVNSYYFEDENHKVNFPK
                     LMEMVAKAAPNMRIRFTTSHPKDLSDDLIEVMAKYKNICKHIHLPVQSGSSKILERMN
                     RKYTREWYLNRIEAIKSKMPDCGLSSDIFCGFSSESEEDFQETLSLMQIVGYDLAFMF
                     KYSERPGTFAANHLDDNIPEEVKKERLARMIKLQNEISLKKNKADIGKTFEVLVEGKS
                     KRSEKMLFGRTEQNKVVVFNAGSAKRGDYVNVKILSASSATLKGEIVNKI"
     gene            complement(1739..2566)
                     /gene="tsf"
                     /locus_tag="PKN53_08895"
     CDS             complement(1739..2566)
                     /gene="tsf"
                     /locus_tag="PKN53_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOD89104.1"
                     /translation="MANITAADVSKLRQMTGAGMMDCKKALVEANGDFERAKELIRER
                     GLAIANKRADREASEGVVLTKLTKNNTVGVMVALSCETDFVAKNVDFIAMAKTILDFA
                     ADNLFENIDQLKEAKINNQSVADMITERSGVTGEKMELAYYEKIEAPLVCAYEHMGNK
                     LATLVGLNKAVDKQIGVNIAMQIAAMNPVAIDKDSVCQKVIDKELEVGREQARNEGKA
                     EELLDKIAQGKLQKFFKENTLLNQEYVQESKTSVREYLQKQDKDLTVTVFKRFGLAN"
     gene            complement(2584..3477)
                     /gene="rpsB"
                     /locus_tag="PKN53_08900"
     CDS             complement(2584..3477)
                     /gene="rpsB"
                     /locus_tag="PKN53_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009162128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOD89105.1"
                     /translation="MPKVTFEELLDAGVHFGHLKRKWNPAMAPYIFMERNGIHIIDLH
                     KTAVKIEEAANAMKNIAKSGRKILFVATKKQAKDIVAEEVKKIDMPYVTERWPGGMLT
                     NFPTIRKAVKKMSSIDKMHTDGTFNTLSKREKLQITRQRAKLEKNLGSIADLSRLPAA
                     LFVVDVMKEKIAINEAAKLNIPVFAMVDTNSDPRVVDFPIPANDDASKSIQLIVRIMT
                     EAIQEGLNERMVEKENTAKPIKDSDDEEEVVAKKRPARKTRARKESIKPKAEEKEETE
                     SKEETTKKSESIDAEAKEENN"
     gene            complement(3592..3978)
                     /gene="rpsI"
                     /locus_tag="PKN53_08905"
     CDS             complement(3592..3978)
                     /gene="rpsI"
                     /locus_tag="PKN53_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOD89106.1"
                     /translation="MKVLNAIGRRKAAIARVYLRSGKGNITINNRKLEDYFTLTQHQH
                     IVKQPFATLGKLEEFDVKANLEGGGIKGQAEALRLGIARALLKVDPEFRASLKAQGFL
                     TRDPRIVERKKPGQPGARKKFQFSKR"
     gene            complement(3983..4438)
                     /gene="rplM"
                     /locus_tag="PKN53_08910"
     CDS             complement(3983..4438)
                     /gene="rplM"
                     /locus_tag="PKN53_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOD89107.1"
                     /translation="MKTLKYRTLSANKETVNKQWFVVDADGEILGRLASKVAFVLRGK
                     HKTDFTPHVDCGDNVIVINAEKIAVTGNKLVDKTYIRHSGYPGGQKQTTLEEMMEKQP
                     GRAVEKAIKGMLPKNALGRQLFRNLYVYVGPEHKHEAQKPTKLDLNTIK"
     gene            5282..5647
                     /locus_tag="PKN53_08915"
     CDS             5282..5647
                     /locus_tag="PKN53_08915"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOD89108.1"
                     /translation="MAKNHDEDLKNLNIIGNGTSIIGNIVSNGDIRIDGSLEGNVETK
                     AKLVLGETGKIKGEIICATSEISGLIEGKISVEDILALKSTAQIIGDIQTTKISIEPG
                     AVFTGTCNMCTNIQDKENI"
     gene            5649..5903
                     /locus_tag="PKN53_08920"
     CDS             5649..5903
                     /locus_tag="PKN53_08920"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOD89109.1"
                     /translation="MENNKDNNNEDKNKKKTGKESLDKFIKYSTLAFEMAIMIGLGTW
                     AGVALDKHFDNSTPGFTIGLSLFSIFGSLYLVIKRVLNDK"
     gene            6368..7450
                     /gene="atpB"
                     /locus_tag="PKN53_08925"
     CDS             6368..7450
                     /gene="atpB"
                     /locus_tag="PKN53_08925"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOD89110.1"
                     /translation="MRRVLFISVIFASLLSIQAFASDAKQVDADEAFDIKGMIMHHLK
                     DSHEWHILDYKKSDGSEKIVSIPLPIIVYANGKLDVFMSSKFEHGNKVVDRGTNKYIL
                     HHDKIYLADENGGLTTEIQDGEEKIMNATPLDISITKNVCAMIIVSIVILWIFISVAK
                     AYEKRPGRPKGLQALMEPLILFVKNDIVYPNLGKKTDKFLPFLLTVFFFILFNNLFGI
                     IPFFPGGANVTGNIAVTMTLATFTFLMINLNGNKHYWQHNLWMPGVPVFVRPILTVVE
                     IMGMFVKPVALTIRLFANITAGHIIILSLVGLIFVFKSLAIAPVTFLFVLFMDVLELL
                     VAFLQAYVFTMLSALFIGIAVAEPEH"
     gene            7487..7687
                     /gene="atpE"
                     /locus_tag="PKN53_08930"
     CDS             7487..7687
                     /gene="atpE"
                     /locus_tag="PKN53_08930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOD89111.1"
                     /translation="MVGLPAIGAALAVIGASYGIGKIGHAAMDAIARQPEAAGNIQTS
                     MIISCALIEGVALFGVVVALLQ"
     gene            7777..8271
                     /gene="atpF"
                     /locus_tag="PKN53_08935"
     CDS             7777..8271
                     /gene="atpF"
                     /locus_tag="PKN53_08935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOD89112.1"
                     /translation="MDLIMPGVGLIFWTSIIFIILLLILGKVAWKPINKMINNRNQSI
                     EDALNMAEKAREEMKQLKAGNEQIMIEARIERDNILKEARELKEQIVAEAKQEAGKEV
                     EKLKKNASMEIAAQKAAAVEEIRNQVLDLSILVAEKVIRREVKDKKDNQVLVDDILKD
                     VKFN"
     gene            8274..8819
                     /gene="atpH"
                     /locus_tag="PKN53_08940"
     CDS             8274..8819
                     /gene="atpH"
                     /locus_tag="PKN53_08940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOD89113.1"
                     /translation="MNQSKISVRYSKAIFESAKEKNLLDKIYEDFQLIKTSIKANENF
                     YSVFTSPVVKSKDKKDVLKNVFSNSINELSMRFLLMLIENKREAYISDIIRDFSSFYR
                     EHKNIKEVVLTTAIDIDKSISQQFANNVEEKLNCDVEIVTNVKPEIIGGVVVRIDNLL
                     LDMSVETQLRNIKSELRIEKY"
     gene            8845..10425
                     /gene="atpA"
                     /locus_tag="PKN53_08945"
     CDS             8845..10425
                     /gene="atpA"
                     /locus_tag="PKN53_08945"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOD89114.1"
                     /translation="MADIKPSEVSQILKQQLEGLSLKTNLEEVGTVLQIGDGIARIHG
                     LDNVQANELIEFENGVKGIVLNLEEDNVGAVLLGHSTDIKEGTTVKRLRRIASIMVGE
                     EMLGRVVNTIGEPLDGKGPIGGELYEMPLERVAPGVIFRQPVNEPLQTGIKAIDSMIP
                     IGRGQRELIIGDRQTGKSTIAIDTIINQKSFYDKGEPVYCIYVAIGQKGSTVASIART
                     LEEAGALDYTVIVTASASEPAAMQFYAPFAGCAIGEYFRDTGRPALIVYDDLSKQAVS
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKIIQSDEIAAKMNDLPESLKGKVKG
                     GGSLTALPIIETQAGDISAYIPTNVISITDGQIFLESDLFNAGIRPAINVGISVSRVG
                     GNAQIKCMKKIAGTLKIDQAQFEELEAFSKFGSDLDAVTLAVIDKGRKNVEILKQGLH
                     SPIKVENQAAIIFCGTNALLRDVPINKISEFEAEYIDYLNLNHKDTMASLARGEYNDE
                     ITGVLTKVAQEFTAKYKK"
     gene            10434..11309
                     /gene="atpG"
                     /locus_tag="PKN53_08950"
     CDS             10434..11309
                     /gene="atpG"
                     /locus_tag="PKN53_08950"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOD89115.1"
                     /translation="MAGLKDIRNRIASVRSTRQITSAMKMVAAAKLKRAQNMVTQIRP
                     YSEKLTEVLQEVSSSLDKNHKNIFINDNESKTVLIVLMASNRGLCGAFNTNVCKKAIS
                     HVEENYAEQAKEGKVKFYCIGKKAIDFIKKSNYEIYKTEIDIFDDLTYSNANKISESL
                     MEIFANEEFERIDLIYNSFKNAAVHEQIVERFLPMTLEVEEKTNPSEYIFEPEINKIL
                     DEMLPKALKIQFFKTILDTNAAEQGARMTAMHQATDNATEMIKDLTLMYNKARQAAIT
                     NELIEITSGAEALKG"
BASE COUNT         3856 a         1690 c         1795 g         3968 t
ORIGIN      
        1 tttcataaga gtaatttttt tattactaat ttatttttct tacttcaaac aattttaaaa
       61 ttcaattttc acacaaattt ggtaaatctc atttaaaaat ttcattcatt aatataattt
      121 acatttcaag gcaaaaatat gctaaaattt tcacataagc ctttaatcta tattacaaat
      181 tagttaagtt ttataatcac acattaatac caaactatat tgtaaaaaat atttaaaatt
      241 taattattgt aaagatatgc ttttttttaa gataaaaacg cagattaatt tattaaattt
      301 tattaacaat ctctcctttt aaagttgcag aactggcact taaaattttt acatttacat
      361 aatcgccacg ttttgcactg ccagcattaa agacaacaac tttattttgt tcggttctac
      421 caaacaacat tttttctgat cttttagatt ttccttcaac taaaacttca aaggttttgc
      481 ctatatctgc tttgtttttc tttaaagaaa tttcattttg cagttttatc attcttgcta
      541 atctttcttt tttcacttct tctggaatgt tatcatctaa atgattagca gcaaatgtcc
      601 caggtctttc agaatattta aacataaaag ctaaatcata acccacaatt tgcattaaac
      661 ttaaagtttc ttgaaaatct tcttcacttt cactcgaaaa accacagaaa atatcagaag
      721 acaaaccaca atctggcatt tttgatttta ttgcttcaat acgatttaaa taccattctc
      781 ttgtgtattt tctattcatt ctttccaaaa ttttactact tccagattgc acaggcaaat
      841 gaatgtgctt gcaaatattt ttatattttg ccattacttc aatcaggtcg tctgataaat
      901 cttttgggtg agaagttgta aatctaattc tcatatttgg agcagctttg gcaaccattt
      961 ccattaattt tgggaaattt actttatggt tttcatcttc aaaataataa gaatttacat
     1021 tttgtcccaa taatgttact tctttaaaat ttttgctttc aagattttta acttcgttaa
     1081 ttatatcaat ataagaactg cttctttctc tgccacgcgt ataaggcaca atgcaatatg
     1141 agcaaaaatt attacaacct ctagtaatag gaacaaatcc agaaatttcg tcttggtttt
     1201 gttttatttg ttcaactcca gaataagttt cgttaatatc gagaattaga tttttttgtt
     1261 ttaaacaatc attattgtca tcaattaatt ctggtaagga tctgtaagca tcaggtccag
     1321 caacaaaatc aacagcgtca tgttcgagaa gtttttcgcc aattctctgt gccatgcagc
     1381 caataatgcc aactttaaga tttttgtttt tcttttttaa ggcttttatg tggtctaatc
     1441 tgttccaaat tttttgttct gcattatctc ttaccgagca agtgtttatt atgatgaggt
     1501 cggcatcatt aattttgttg actccaaaat aattttttgc tatcataatt cctgcaacag
     1561 tctctgagtc tgcaacattc atttgacagc cataggtttc tatataaatt ttcttattca
     1621 taaggtttaa taatgcttgc aaaaataata aacatcttga agctaataaa ttattaaata
     1681 aaaaaagtct cgaacaaaat gccgagactt tttttaacat taaacaaaat attaattttt
     1741 aatttgccaa tccaaatctt ttaaaaacag ttactgtaag gtctttatct tgtttttgta
     1801 gatattctct tactgatgtt tttgattctt gaacatattc ttgatttagc aaagtatttt
     1861 ctttaaagaa tttttgaagt ttaccttgag caattttgtc aagaagttct tctgctttac
     1921 cttcgttgcg agcttgttcg cgtccaactt ctaattcttt atcaataact ttttggcata
     1981 cagaatcttt atcaatagca actgggttca ttgctgcaat ttgcatagca atgttaacgc
     2041 ctatttgttt gtcaacagct ttgtttaatc ctactaaagt tgctaattta tttcccatat
     2101 gttcgtatgc acaaacaaga ggagcttcaa ttttttcgta ataagccaac tccatttttt
     2161 cgccagtaac accacttctt tcagtaatca tatcagcaac actttggttg ttgatctttg
     2221 cttctttaag ttggtctata ttttcgaata aattgtctgc tgcaaaatct aaaattgttt
     2281 tagccatagc aataaaatct acgttttttg caacaaaatc ggtttcacaa ctaagtgcaa
     2341 ccattactcc tacagtattg tttttagtaa gttttgttag tacaacacct tcagaagctt
     2401 ctctgtcggc tcttttgtta gcaatagcta atcctctttc gcgaattagt tcttttgctc
     2461 tttcaaaatc accattagct tcaactaagg cttttttgca gtccatcatg cctgcaccag
     2521 tcatttgtct taatttgctt acatcagcag ctgttatatt tgccatttta tcaaatttta
     2581 aaattagtta ttttcttctt tagcttcagc gtctatgctt tcactttttt tagttgtctc
     2641 ttctttactt tcggtttctt ctttttcttc tgctttaggc tttatgcttt cttttcttgc
     2701 tctagttttg cgagcaggtc tttttttagc aacaacttct tcttcatcat cgctatcctt
     2761 aataggtttt gcagtatttt ctttttcaac cattctttcg tttaaacctt cttgaattgc
     2821 ttcagtcatg attcttacga taagctgtat tgattttgat gcatcgtcgt ttgctggaat
     2881 aggaaaatca accactctag gatcgctatt agtgtcaacc atagcaaaaa cagggatgtt
     2941 taattttgca gcctcgttaa tagctatttt ttctttcatc acgtcaacaa caaaaagtgc
     3001 agcaggaagt cttgacaaat cagcgattga acctaagttt ttttccaatt ttgctctttg
     3061 acgagtaatt tggagttttt cacgttttga caaggtgtta aatgttccgt cagtgtgcat
     3121 tttgtcaata gaagacattt ttttcacagc tttacgaata gttgggaagt ttgttagcat
     3181 accaccgggc caacgttcgg taacgtaagg catgtctatt tttttaactt cttcagcaac
     3241 aatgtctttt gcttgttttt ttgttgctac aaataaaatt ttacgaccag atttagcaat
     3301 gtttttcatt gcattagcag cttcttcaat tttcactgca gttttgtgta ggtcgataat
     3361 gtgaataccg ttacgctcca taaaaatata aggagccata gcaggattcc atttacgctt
     3421 taggtgtccg aaatgtacac cagcgtcaag taattcttca aatgttactt ttggcatttt
     3481 ttttaaattt aagtttacat tctgtagtaa caattatgag gtagaatctt gaacaacaaa
     3541 aactgtcccc ataatttaga tactaaactt tgtttacact cactaaaaat attaacgttt
     3601 actaaattga aatttcttac gagcaccagg ttgtccaggt tttttccttt caacaattct
     3661 tggatctcta gtaagaaaac cttgagcttt taagctagca cggaattcag gatctacttt
     3721 aagcagtgct cttgctatgc ctaaacgcaa agcttcggct tgtcctttaa ttccaccacc
     3781 ttctaaattc gctttaacat caaattcttc taatttacct aatgttgcaa aaggttgttt
     3841 tacgatatgt tgatgttgag ttagcgtgaa ataatcctca agttttctgt tgttaattgt
     3901 aatatttcct tttccgcttc ttagatatac tcttgctata gcggcttttc tacgtcctat
     3961 tgcatttaaa actttcataa tactatttaa ttgtatttaa atccaattta gttggctttt
     4021 gagcttcgtg tttgtgctca ggaccaacat aaacatataa atttctaaat aattggcgac
     4081 ctaaagcgtt tttaggcaac attcctttta ttgctttttc aactgctctg cctggctgtt
     4141 tttccatcat ttcttctaat gtggtttgct tttgaccacc tggataacca gaatgtctaa
     4201 tataagtttt gtcaactaac ttgttgccag taactgcaat tttctcagca ttaataacaa
     4261 taacattatc tccacaatca acatgaggag taaaatctgt tttatgtttg ccacgaagaa
     4321 cgaaagccac tttagacgct aaacgcccta aaatttcacc atctgcatca acaacaaacc
     4381 actgtttatt tacagtttct ttgttagctg ataatgttct gtacttcaat gttttcacaa
     4441 ttttaaaatt ttaaataata ataaattatt ttttacatta ggctggatga ccaaacggac
     4501 tgcaaaaata gtattttttt tatttaaaca agaatttttt aataaataaa aaataaatct
     4561 acaacaactc cgtttgcctc taatagcaat atgtgtagaa aaattggttt tgtaaaaaaa
     4621 ctttttttct aaagtttaat cttttttatt tgtcgataag caagcaaaaa ttaattgttt
     4681 tgagcaaatt ttgtgaaata tttttattaa caatttttgt ctatcttatt taataagttg
     4741 agttatgtgt caaaataagg tttaagacaa atataattgt taaagaaaaa aaccattaat
     4801 taattgtata agttttagaa agatgaataa aaatttgttt gagcttgata attcattaga
     4861 tattttacat aatagtatcg cctgtttttc gttttttttg aaccacacgc taactcattt
     4921 aattgtccga aggacaatat aagactatca agggtttgtc cgtaggacaa catgtgatta
     4981 accgtaggcg tagcccgcgg tatttaaacc ctcgaaagcc ttgcccgtag ggcaacacaa
     5041 taaactcagt taaaaattta taaagtaaaa agtttgtaaa gaaatgccaa acagaaaacc
     5101 gcttttaact tttaactggg gtgcacttat gggcagggcg taattgttcg gtatggggct
     5161 gaaacgctga aaataagtat tcgttaggta tttttataca atagcattaa aaaataccta
     5221 atattttttt cattttgtaa acaatgtttt tatatttgcc taagaaaaac aaaacaatat
     5281 tatggcaaaa aatcacgacg aagatttaaa aaatcttaat attattggta atggaacttc
     5341 cataattgga aatattgttt caaatggaga tattagaatt gatggtagtt tagaaggaaa
     5401 tgtagaaact aaagctaaac ttgttctagg agaaactggt aaaataaaag gagaaattat
     5461 ttgtgcaaca agcgaaattt ccggacttat agaaggaaag attagcgttg aagatatttt
     5521 ggctttgaaa tctacagcac aaataatagg tgatattcaa actacaaaaa tttctattga
     5581 gccaggagct gtttttacag gaacttgtaa tatgtgtaca aatatccaag ataaagaaaa
     5641 tatataaatt ggaaaataat aaagacaaca ataacgaaga taaaaataaa aaaaaaacag
     5701 gaaaagaaag cttagataag tttataaaat actctacctt agcttttgaa atggctatta
     5761 tgattggttt aggaacatgg gcaggagttg ctttagataa acatttcgac aattctactc
     5821 ctggattcac tatcggtttg tctctttttt caatttttgg atccttgtat cttgttataa
     5881 aaagagtgtt gaatgacaag taaagaacaa tttaaacttt ttataaactt tagctttgta
     5941 gcaatattaa tttttgcagt acattatttt ttattgtttt attttagaag atatttttat
     6001 gctcctaatg tactttggat aaaccctttt ttatttgtgt taagtttttt ttctattctt
     6061 attataagtt taatattgag aaaaaataga agaaaaaata taacaaatgt atatatgctt
     6121 accatattgg gaaaaattat tttttctctc ttgtttttgc taccactttt tataaataat
     6181 agtgttttta aaaaagaatt tgtaatacag ttttttatat tttatttcgt ttatctaata
     6241 attgagctgt tttgtttgat aaaaataacc aaaaatggtt aattgaaaaa aaatgacaaa
     6301 taatattagg atattaaaat tgaagttgta aatttgtatg ctacattaaa aaactttttt
     6361 gattgtaatg agacgagtat tatttataag tgtaattttt gcaagtttgt tgtcaataca
     6421 agcttttgca tcagacgcaa aacaagtaga tgcagatgag gcttttgata ttaaaggaat
     6481 gataatgcac catttgaaag actctcacga atggcatatt ctagattata aaaaatctga
     6541 tggaagcgaa aaaattgttt ctattccttt accaataatt gtgtatgcaa atgggaaatt
     6601 agatgttttt atgtcttcta aatttgaaca tggcaataaa gttgtggata gaggaacaaa
     6661 taaatatata cttcaccacg ataaaattta tttagctgat gaaaatggag gattgacaac
     6721 cgaaattcaa gatggcgaag aaaaaataat gaatgcaaca cctttagata tttctattac
     6781 taaaaatgtt tgtgcaatga ttatagtttc cattgttata ttatggatat ttatatctgt
     6841 agcaaaagca tacgaaaaac gtcctggaag accaaaaggt ttgcaagccc ttatggaacc
     6901 attgatactg tttgtaaaaa atgatatcgt ttatccaaac ttaggcaaga aaacagataa
     6961 gtttttaccg tttttgctaa cagtcttttt ctttatttta tttaataatt tatttggtat
     7021 aattccattt ttcccaggtg gggcaaatgt aacaggtaat atcgctgtta caatgacttt
     7081 agcaacgttt acctttttaa tgataaactt gaatggaaac aaacattatt ggcaacataa
     7141 tttgtggatg cctggtgtgc cagtttttgt tcgtccaatt ttaacagtag ttgaaattat
     7201 gggaatgttt gttaagccag tggctttaac cattcgtctt tttgcaaata taactgcagg
     7261 acatattatc attttgagct tagtgggttt aatatttgtt tttaaatcat tagcaatagc
     7321 tccagtaact ttcttattcg tactttttat ggacgtttta gagttgctgg ttgcattttt
     7381 acaggcatac gtatttacaa tgctttctgc tttgtttata ggcatagcag tagcagaacc
     7441 agaacattag aatttttttt atgtttaact taaataattt aaaattatgg taggacttcc
     7501 agcaatcggc gctgcactag cagtaattgg tgcaagctat ggtattggaa aaattggaca
     7561 cgcagcaatg gacgcaatag caagacaacc tgaagctgca ggtaacatcc aaacttcaat
     7621 gattatttca tgcgctctta tcgagggtgt ggcattattt ggcgttgtag ttgctttgtt
     7681 acagtaaaac aattttacat ttcccgacta gcgattggct acgggaaatg ttttttaaaa
     7741 gtaatttata aaaaaataat ataaaataat tgcagtatgg atcttataat gcccggtgta
     7801 ggtttaattt tttggacaag tataattttc ataatattat tacttatcct tggaaaagta
     7861 gcatggaaac ctatcaacaa gatgattaac aacagaaatc aaagtattga agacgctttg
     7921 aatatggcag aaaaagctcg cgaagaaatg aaacagctaa aagctggcaa cgaacaaatt
     7981 atgattgagg caagaataga aagagacaat attcttaaag aagctagaga attaaaagaa
     8041 caaatcgttg cagaagctaa gcaagaagca ggaaaagaag ttgaaaaact aaagaaaaat
     8101 gcttcaatgg aaatcgctgc acaaaaagct gctgctgttg aagaaatcag aaaccaagtc
     8161 ttagatttgt caattttagt cgccgaaaaa gttatcagac gagaagtaaa agacaaaaaa
     8221 gacaatcaag ttttggtaga tgacatttta aaagatgtaa agtttaatta attatgaatc
     8281 aaagtaaaat atcggtaagg tattcgaaag caatttttga atcagcaaaa gagaaaaatc
     8341 ttcttgataa aatttatgaa gattttcaat taattaaaac aagcattaaa gcaaatgaaa
     8401 acttttatag cgtttttaca agtcctgtag taaaatctaa ggataaaaaa gatgtgttga
     8461 aaaatgtgtt ttcaaattct attaacgaat taagcatgag gtttttactc atgttaatag
     8521 aaaacaaaag agaagcttac atatctgata ttattcgtga tttttcatct ttttacagag
     8581 aacataaaaa cataaaagaa gttgtgttaa caacagctat agacattgac aaatctattt
     8641 ctcagcaatt tgctaataat gttgaagaaa aattaaattg cgatgtagaa attgtaacta
     8701 atgttaaacc tgaaataatc gggggcgtag ttgtaagaat tgacaattta ttattagata
     8761 tgagtgttga aactcaatta agaaatatta aatcagagtt aagaatagaa aaatattaaa
     8821 aattaaaaaa ataatatagc agttatggca gacattaaac catcagaagt atcacaaatt
     8881 ttaaaacaac aattagaagg tttaagttta aaaacaaacc ttgaagaagt tggaactgtt
     8941 ttacaaattg gagacggtat tgctcgtatt catggcttgg ataatgttca agcaaatgaa
     9001 ttaatagaat ttgaaaatgg agttaaaggt atagtattaa atcttgaaga agataatgtt
     9061 ggagctgtgc ttttgggtca tagtacagat ataaaagaag gtactactgt taaaagatta
     9121 agacgtattg cttcaattat ggtgggtgaa gaaatgctag gtagagttgt taatactatc
     9181 ggagaaccat tggacggtaa aggtcctata ggtggcgaat tatacgaaat gcctcttgaa
     9241 agagttgctc caggtgttat ttttcgtcaa cctgtaaacg aacctttgca aacaggtatt
     9301 aaagcaatag actctatgat tcctatcgga cgtggtcaac gtgagcttat tatcggcgac
     9361 cgtcaaacag gaaaaagtac aattgcgata gatactatca taaatcaaaa atctttttat
     9421 gataaaggcg aacctgtata ttgtatttat gtagcaatag gtcaaaaagg ttctacagtt
     9481 gctagtatcg caagaacttt agaagaagca ggtgctttag attatacagt tattgttacc
     9541 gcttcggctt ctgagccagc agcgatgcag ttctatgctc cattcgctgg ttgtgctatt
     9601 ggtgaatatt tccgcgacac aggacgtcct gctttaattg tttacgacga cttgtcaaaa
     9661 caagcagttt catatcgtga agtgtcgttg ttgctaagaa gacccccagg acgtgaagca
     9721 tatcctggtg acgttttcta cttgcactca agattgcttg aaagagccgc taaaattatt
     9781 caatctgatg aaattgctgc aaaaatgaac gacctacccg aaagccttaa aggtaaagta
     9841 aaaggtggtg gttcgcttac tgctcttcca ataatcgaaa cccaagcagg tgatatttct
     9901 gcatatattc caacaaacgt tatttcaatt acagacggac aaatcttctt agagtctgac
     9961 ttgtttaatg caggtattcg tccagctatt aatgttggta tttctgtatc tagagttggt
    10021 ggaaatgctc aaattaaatg tatgaaaaaa attgcaggaa cattaaaaat tgaccaagct
    10081 caattcgaag agttagaagc tttctcaaaa tttggttccg accttgatgc tgttactctt
    10141 gcagtaatcg acaaaggaag aaaaaatgta gaaatattaa aacaaggact acattctcct
    10201 attaaagtag aaaatcaagc agcaattatt ttctgtggaa caaatgcctt gttaagagat
    10261 gtaccaataa ataaaattag cgaatttgaa gctgaatata ttgattactt gaacttaaat
    10321 cataaagata caatggcaag ccttgcaaga ggtgaatata acgatgaaat tacaggggtt
    10381 ttaacaaaag tagcacagga atttacagct aaatataaaa aataaattaa ttcatggcag
    10441 gattaaaaga tatacgtaat agaattgctt ctgtaaggtc aacaaggcaa attacttcag
    10501 ctatgaaaat ggttgcggct gctaagttga aaagagctca aaatatggtt acgcaaatta
    10561 gaccttattc agaaaagcta acagaagtgt tacaagaagt aagttctagt ttagataaaa
    10621 atcataaaaa catttttatt aacgataatg aatctaaaac tgtacttatt gttctaatgg
    10681 cttcaaacag agggctttgt ggtgcgttta atacaaatgt ttgtaaaaaa gctattagcc
    10741 atgtagaaga aaactatgca gaacaagcaa aagaaggaaa agttaaattt tattgtatag
    10801 gtaaaaaagc tatagatttt ataaaaaaat caaattatga aatctataag actgaaatag
    10861 acatttttga cgatttaact tattctaatg caaataaaat tagcgaatca ttgatggaaa
    10921 tatttgctaa tgaagagttt gaaagaatag acttaattta caatagcttt aaaaacgccg
    10981 ctgttcacga acaaatagta gagcgatttt taccaatgac actagaagtt gaagaaaaaa
    11041 ctaatccatc tgagtatatt ttcgagccag aaattaataa gatcttagac gaaatgcttc
    11101 ccaaagcttt aaaaattcaa ttttttaaaa ctattttaga tactaacgct gcagaacaag
    11161 gagctagaat gacagcaatg catcaagcaa ctgataatgc aacagaaatg attaaagatt
    11221 taactttaat gtataataaa gctcgtcaag cagctattac aaacgaactt atcgagatta
    11281 ctagtggtgc agaagcactt aaaggataa
//
LOCUS       DAOPNX010000085        11203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_6672_length_11203_cov_6.548977, whole genome shotgun sequence.
ACCESSION   DAOPNX010000085 DAOPNX010000000
VERSION     DAOPNX010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11203
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6672_length_11203_cov_6.548977"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1595)
                     /locus_tag="PKN53_08955"
     CDS             complement(60..1595)
                     /locus_tag="PKN53_08955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD89116.1"
                     /translation="MKRVFVKLIFAVLLLLVCVNVTLAQTEVLEESCPGVTNLTATIN
                     PYNTVTLNWTSPPRSEWPAEYIEYLIYDGENRIGNGNVYVDTVELNSWTTYVLGLGTH
                     NLGVEMRYFTDYVHLACSSDTASVEVVITETYICPPITNLTATVNSDNTVTLNWTNPP
                     QNELPSDKPIYWFFDGETNIGANWKGVLTSWQTPILEQGIHNLGVRVSYLEGCYLCTT
                     DTIFVEAHITKNYTCPSVKKLEAKVNADKTVTLRWKNPDPRASNWPSACSGNLNFRFY
                     LGNTYLGDNYSDNLTTWTSDKTLPNGTHKFRVIIRYVASNGFSEVCESEASTIVTVKG
                     SFDCPSVKDFNTIENEDSTINLSWTLPTELPDGCNGDIGFYFWSDFRYLGYDDTDDLT
                     SWTTPVISVDKPKLTVVIYYYDDNRKTICFAEANNGNDLNMPLFSAEIFPNPANDILT
                     IKCDSEIVSYELYDALGRLVQNKTEVANAESHVNLSSLKQGMYMLRLNTVNGSGTFKI
                     IKN"
     assembly_gap    1837..1936
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1937..2567)
                     /locus_tag="PKN53_08960"
     CDS             complement(<1937..2567)
                     /locus_tag="PKN53_08960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD89117.1"
                     /translation="MEKIALDYVENYCEPEDELLKELSRETALTQLHHRMLSGNYQGH
                     LLAMFVKLFNAKSVLEIGTYAGYSTICIARALPENGKITTIEVNDEIQWLSEKYFEKS
                     GMKNKINALLGDAKEIIPNLNHEFDLVFIDGDKREYIEYYNLVFPKLRKGGLILADNV
                     LWNNKIFGKIESNDYMTKGILKFNEYIAQDKRVEKLILPVRDGLMMIIKN"
     gene            complement(2577..3794)
                     /locus_tag="PKN53_08965"
     CDS             complement(2577..3794)
                     /locus_tag="PKN53_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOD89118.1"
                     /translation="MYYTGLLNNGIRIIHRYVDSYVSHCGIIINCGTRDEDFNQHGLA
                     HFVEHMLFKGTKKRKAFHVLSRMDDVGGDIDAWTTKEETVIAASFLTEHYERSIELLN
                     DVVFNSAFPQKELEKEREVIIDEINSYKDSPSDSIFDQFDELLFPDNSLGRNTLGAKS
                     KLLKYNSDDLLNFVKEKYNTEQMVFCSIGKIKFEKLVKYCEKYFGQNPENQRLYQREK
                     FETKTVFNKSVKKNTYQAHCVLGTYAYSFKDNKRLLLSMLSNLLAGPGMNARLVMMLR
                     EKKGISYNIESSYVSYEDTGCFNIYFGSDKDKLDLALEIIKKELIKLKENKLGSLQLS
                     KLKKQFVGQLAISSEIHSNTLLSMGKTYLIYNQVETLTDVLKEIDTVTSEQLLEVANE
                     IFDLDKFSTLIYY"
     gene            3935..4351
                     /locus_tag="PKN53_08970"
     CDS             3935..4351
                     /locus_tag="PKN53_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOD89119.1"
                     /translation="MKSYRKILYFETSQRREFINITQDVANCLYESKIKEGLCLVNAM
                     HITASVFINDDESGLHQDFEKWLEELAPEKPYDRYKHNTYEDNGDAHLKRTIMGREVV
                     VAITEGKLDFGPWEQIFYGEFDGMRRKKVMVKIIGE"
     gene            4354..5055
                     /locus_tag="PKN53_08975"
     CDS             4354..5055
                     /locus_tag="PKN53_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89120.1"
                     /translation="MRKNILYLLLILLIFSFSCKNKSSENLSEETTDSAKEFSVPDTY
                     KLISSTKGDLDKDNIDEIIYLYLTNEDKDTNGFDRKIYICKKENNKTIIWKEITGPVL
                     SADYLHQNIDPFEEILIDENKIYIIHSVEAENKFYFNHCFEFKDNDFYLTKATVDYAI
                     PCEKWDTYIIDFNTKIVSHQSSPDWCDDEPIGKKFQMSKQEYKVEDLQSYKMQDFIPG
                     NNKLIIPNTKIEVYF"
     gene            5117..5770
                     /locus_tag="PKN53_08980"
     CDS             5117..5770
                     /locus_tag="PKN53_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89121.1"
                     /translation="MKKYFLILIFALASQLVVAQEDKFYFGDENHFSNTNNTEQEKKI
                     PFKTKTNTNKFRTSFSAGTGFSSFYGQNLFYNYVSPKIDYNFTDRFTLSAGTAFVHSN
                     FPSYLDWNAENRTSKAMNQNKLYFFMKGSYLVNDRLRIHASSIFDTNPVAISNNANNA
                     FTSFGFDYKIGENSYFSAEISIHRNPFLRQYDPFYFDNPLVNRPFGNSLFSDPFPQW"
     gene            5764..7572
                     /locus_tag="PKN53_08985"
     CDS             5764..7572
                     /locus_tag="PKN53_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOD89122.1"
                     /translation="MVNNTTLSFPTSKKEMQTLGWQAADIILVSGDAFVDHPSFGTAV
                     IARVLEKEGFKVAVIPQPNWQDDLRDFKKLGKPNLFFGVSAGNMDSMINHYTAAKRKR
                     SDDAYTAGGKAGQRPDYASNVYSNILKQLYPETPVILGGIEASMRRFAHYDFWQDKIL
                     NSILISSKADLLVYGMGEQSIREIAKQLKETNNIKSCYNIKQCAYIGEIDDIQDSDIE
                     LFSFQEVVKDKLKYAANFVKIENETNYNSNKRITQITENKKIIVNPAFPALSEKEMDE
                     IYDLPYTRLPHQRYSKKQAIPAYEMIKNSVNIHRGCFGACSFCTIAAHQGKSIASRSK
                     KSILNELQKIAEMPDFKGHITDIGGPSANMYKMQGFDLNICKTCKRPSCIFPSVCKNL
                     NTSHEELLNLYQDALKIKGITKISIGSGIRYDIAIHETKNPKTNKINQKYLELLISKF
                     VSGRLKVAPEHCSPEVLKLMRKTSFSGFKKLRKIFFNVNDKYELNQQIIPYFISSHPG
                     CKEEHMAELAKELKSIGYKPEQVQSFTPTPMTLSTVMYYTGINPYTGEKLYIAKSQKE
                     KDKQLSYFFWYKPENRKSKKERTNKNISIKNRNKTR"
     gene            7726..8367
                     /locus_tag="PKN53_08990"
     CDS             7726..8367
                     /locus_tag="PKN53_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89123.1"
                     /translation="MRTFNLKIFFLLFCLTLVVNVFSQSTIQLKFSPIGIHPFNETNK
                     HLFDNKIDENAIFVTEPLFLLCYESYLREDSFAFRGMFGFLNDAAAKPAITLHLGLKQ
                     RLVQIWRNSFSVGAGFNLYGRGLWSTIPGYIQEKHWGANGTWEYKLGFMGELEYALFL
                     NDKNDLTLSLLYGHQQNTFTVTIGYKYWISSIIKHPKKCGNCPFQKTSKHWNP"
     gene            8401..10293
                     /locus_tag="PKN53_08995"
     CDS             8401..10293
                     /locus_tag="PKN53_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOD89124.1"
                     /translation="MEIKRVFDILELYNNEYAELKDAFSYKEKGSWVNFSAQDYYKYS
                     HLFACGLINKGFKKGEKIITMSQSLPHWNIADMGISLAGLVNVPIYTTLGGEELEYIL
                     RHSDAKIIIVSDQNLYKKLKPIADKIPEIQTVFTFHHIEGIENWVSLIQEAQENEEEL
                     MKKLEERKKSVDENDMHTLIYTSGTTGNPKGVMISHKNILSNALAVKPIVPIDHGQRA
                     LSFLPLCHIYERMLNYCFQIKGISIYYAENLGTIANDLKDIKPHLFNSVPRVLEKFYD
                     RIISVGKDLKGIKKWIFFAAVRLGTKYDDKGKNSLMYRFKLGIYRKLVFNKWKAALGG
                     NIVAVVSGGASLQVRLARLYTAAGVRVCEGYGLTETSPVLAVNWPDWNKSRLGTVGPI
                     LEGVQVKIAEDKEILAKGPNIMLGYYKNEEETKKVIDEDGWFHTGDIGEFIDNKYLKI
                     TDRKKETFKTSSGKYIAPQVIENKFKTSNLIEQLMVVGENEKFVSALISPNFNYLHFY
                     ASKHKIHYRENSDLIKHSEIINKIQKEINLVNETLGEHERIKRFRLVCEEWSQQTGEL
                     SPTLKLKRNVIYKKYAHILEEIYGHGEDKTENMGKEGPGPKETFEKKISNGMKKLINI
                     YSSNKE"
     gene            10335..11117
                     /locus_tag="PKN53_09000"
     CDS             10335..11117
                     /locus_tag="PKN53_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOD89125.1"
                     /translation="MNSGFIKVNNKNIFYKFINSEFFDNTDDVLIFLHEGLGSIGQWK
                     EFPQKLSDNLKIPALVYDRYGYGKSDGVSESRTLNYLEEDAENFMPQIFNKLLKPNSK
                     KILIGHSDGGSLAILYAAMFPNDTKAIITEAAHLFVEEITYNGVKKMYDIFYEKNLKK
                     RFEKYHGENTVSMFHGWHDVWTNPNYFSWNIEKYLKLIQCPVLAIQGDKDEYGTLKQL
                     ESLENNIKSPCQIEIIKDCGHIPHFEAEKEVKNIMTNFIMNL"
BASE COUNT         4116 a         1745 c         1628 g         3614 t
ORIGIN      
        1 tttgcaaggc tttgttttta attctagata attagttgcg gatttaagga actttttaac
       61 taattcttaa ttattttaaa ggttccgctt ccattcacag tatttagtct aagcatatac
      121 attccttgtt ttagcgaaga taaatttaca tgactctcag cattagccac ttctgtttta
      181 ttttgcacca atctaccaag tgcatcataa agttcgtagc taacaatttc gctatcgcat
      241 ttaatggtta gtatatcatt tgctggattt ggaaatattt ctgctgagaa aagaggcata
      301 tttaggtcgt tgccattatt tgcttcggca aaacatatag ttttgcgatt atcatcataa
      361 tagtaaataa caacagttaa tttaggttta tctactgaaa ttacaggcgt tgtccacgaa
      421 gttaaatcgt cagtatcatc gtagcccaaa taccggaaat cactccaaaa ataaaagcca
      481 atatcgccat tgcaaccatc tggcaattct gttgggagtg tccaacttaa atttattgta
      541 ctatcttcat tttctattgt attaaagtct ttaacagatg gacaatcaaa actaccttta
      601 acagttacta tagtactagc ttcactttca caaacttcag aaaaaccatt gcttgcaaca
      661 taacgtatta taactctaaa tttatgtgtc ccatttggaa gagttttatc gcttgtccaa
      721 gttgttaaat tgtctgaata attatctcct aaataagtat taccaagata aaatcgaaaa
      781 ttaagattac cagagcaagc gcttggccaa ttgcttgccc ttgggtcggg atttttccag
      841 cgaagtgtta cagttttatc tgcatttact tttgcttcca gttttttaac tgatggacaa
      901 gtatagtttt tagtaatatg tgcttctaca aaaatagtat cagttgtgca aagataacac
      961 ccttctagat aacttactcg tactccaaga ttgtgaatcc cttgttcaag tattggcgtt
     1021 tgccatgaag ttaatactcc tttccaatta gcgcctatat tagtttcacc atcaaaaaac
     1081 caatatattg gcttgtcgct tggcaactca ttttgtggtg gatttgtcca attaagagtt
     1141 actgtattgt ctgaatttac tgttgctgtg aggttggtaa taggtggaca aatataagtt
     1201 tcagtaatta ctacttctac agaggcggta tcggaagaac aagctagatg tacataatca
     1261 gtaaaataac gcatttcaac tccaagattg tgagttccta atccaagtac atatgtcgtc
     1321 catgagttta attcaacagt atcaacataa acattaccat tgcctatacg attttctcca
     1381 tcataaatca aatattctat atattcggct ggccactcac ttcgcggtgg gcttgtccag
     1441 tttagggtta ctgtattgta tggatttatt gttgctgtta ggttggttac acctggacaa
     1501 gattcttcta aaacttctgt ttgtgccaat gtaacattaa cacaaactag taacaacaac
     1561 acagcaaaaa ttaattttac aaaaactctt ttcatactaa aattaaactc gagttaatga
     1621 ctaaaattga ttataataaa caattttaag tttctgtaaa gataatgctt tttaaaaaat
     1681 aatacaattt tattagttaa aattctcaaa cctctattgt ctgaattttc aagtaattct
     1741 taactattca attttcttaa gagcgaatgc agagccaagc ttgcttgagc tatgccgaga
     1801 gacgacagaa aatgcagcga agctgaatta tcaattnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnaatt cttaattatc atcatcagtc catcacgaac aggcaaaatc
     1981 aatttttcaa ctcgtttatc ttgagcaata tattcattaa attttaaaat tccttttgtc
     2041 atatagtcat ttgattcaat tttgccgaaa attttattat tccacaaaac attatcagct
     2101 aaaatcaaac cgccttttcg caattttgga aaaactaaat tataatattc tatatattct
     2161 cgcttgtcgc cgtcaataaa tactaaatca aattcgtggt ttaaatttgg aattatctct
     2221 tttgcatcgc ctaataatgc attaatttta tttttcattc ccgacttttc aaaatatttt
     2281 tccgaaagcc attgaatttc atcattcact tcaattgttg ttatttttcc attttcgggc
     2341 aatgctcttg ctatacaaat agttgaataa cctgcataag tacctatttc taatactgat
     2401 ttagcattaa aaagtttaac aaacatagct aacaaatgtc cttggtaatt tcccgaaagc
     2461 attctatgat gcaattgtgt tagtgcagtt tcacgcgaaa gttcttttaa caattcatct
     2521 tcgggttcgc aataattttc aacataatcc aaagcaattt tttccatatt ttccttttaa
     2581 taataaatta gagttgaaaa tttatccaaa tcaaaaattt cgtttgcaac ttcaagcaat
     2641 tgttctgacg ttactgtatc aatttctttt aaaacatctg ttagcgtttc tacttgatta
     2701 taaataagat aggttttccc catagacaac aaagtattgg aatgaatttc gctacttatt
     2761 gcaagttgcc caacaaactg ctttttcaac tttgataatt gtaaacttcc caacttattt
     2821 tctttcaact ttatcaattc tttttttata atttctaaag ccaaatcaag tttatcttta
     2881 tcagacccaa aatatatatt aaaacagcct gtatcttcat atgaaacata acttgactca
     2941 atattataag aaattccttt tttctctcgc aacatcatta ccaatcttgc attcattcca
     3001 ggaccagcaa gtaagtttga caacatagac aatagcaaac gcttattatc tttaaaagaa
     3061 taagcataag ttcctaaaac acaatgagct tggtatgtgt tcttttttac tgatttatta
     3121 aaaactgttt ttgtttcaaa tttttctctc tgataaagtc gctgattttc aggattttga
     3181 ccaaaatatt tttcacaata ctttactaat ttttcaaatt taatttttcc aatagaacaa
     3241 aataccattt gctcagtatt gtatttttct tttacaaaat tcaataaatc atcggaatta
     3301 tattttagca gttttgattt tgctcctaaa gtattgcgtc ccaaagagtt atctggaaat
     3361 agcaattcat caaattggtc aaaaatactg tcagaaggcg aatctttata agaattaatt
     3421 tcatctatta ttacttctct ttccttttcc aactcttttt gtggaaatgc agaattaaaa
     3481 acaacatcat ttaataattc tattgaacgt tcataatgct cggttaaaaa acttgcagca
     3541 attactgttt cttctttggt tgtccatgca tctatatcac cgcctacatc gtccatacgg
     3601 ctaagaacat gaaatgcttt tcgttttttc gttcctttaa aaagcatatg ttccacaaaa
     3661 tgagccaagc catgctgatt aaaatcttcg tctcgagtgc cacaattaat tattatgcca
     3721 caatgtgaaa cataagaatc cacataacga tgaattattc ttattccatt atttaataaa
     3781 cctgtatagt acaaatcttt aaatttttga caaagataca attctaaaat attctaataa
     3841 aattattttt tacacttaaa aaatttaata tctgatacaa tagtttaaaa gctatttttt
     3901 attaactttg taacgaattt acaaactaaa ttttatgaaa tcataccgaa aaatattgta
     3961 ctttgagaca tctcaacgcc gtgaatttat aaatatcact caagatgttg ccaactgtct
     4021 ttacgaaagc aaaattaaag aaggcttgtg cttagtaaat gcaatgcaca ttaccgcaag
     4081 tgtatttata aatgatgacg aatctggttt acatcaagat tttgaaaaat ggttggaaga
     4141 gttagctccc gaaaaacctt acgacagata caaacacaac acttatgaag acaatggcga
     4201 tgcacatcta aaaagaacaa ttatgggtag agaagttgtt gtcgcaatta cagaaggtaa
     4261 actcgacttt ggaccttggg aacaaatttt ttatggcgaa tttgatggta tgagacgtaa
     4321 aaaagtaatg gttaaaatta ttggcgaata attatgagaa aaaacatttt atacctatta
     4381 ttaattttgc taatattttc attttcatgc aaaaataaaa gcagtgaaaa ccttagtgaa
     4441 gaaactaccg actctgcaaa agaattttct gtaccagata cttataaatt aatttcaagt
     4501 acaaaaggcg atttggataa agataatatt gatgaaatta tttacctata ccttaccaac
     4561 gaagataaag acacaaatgg ttttgataga aaaatttata tttgtaaaaa agaaaataac
     4621 aaaactataa tttggaaaga aattacagga cctgttttaa gtgcagatta tttacaccaa
     4681 aatattgatc cttttgaaga gattttaatt gatgaaaata aaatttacat tatacactct
     4741 gtagaagctg aaaataaatt ttatttcaac cactgttttg agtttaaaga caacgatttt
     4801 tatttaacaa aagcaactgt tgactacgct atcccatgcg aaaaatggga tacttatatt
     4861 attgacttta acacaaaaat cgtttcacat cagtcatcgc cagattggtg tgatgatgaa
     4921 cctattggta aaaaatttca aatgtctaaa caagaatata aagttgaaga tttacaatcc
     4981 tataaaatgc aagattttat acccggcaac aataagctaa taattccaaa tacaaaaatt
     5041 gaagtttatt tttaaaaaaa taaatcctct cttttgtaat tattcagaaa tttaatatct
     5101 ttacaaaaat tattttatga agaaatattt tttgatatta atttttgcat tagcttctca
     5161 acttgttgtt gcacaagaag ataagtttta ttttggtgat gaaaatcatt tttccaacac
     5221 caataataca gagcaagaaa agaaaatacc ttttaaaaca aaaacaaaca ctaataaatt
     5281 tcgtacaagt ttttcagcag gaacaggttt ttcaagtttt tacggacaaa atcttttcta
     5341 taattatgtt tcgccaaaaa tagattacaa ttttactgac aggtttactt tatcggcagg
     5401 aacagctttt gtacactcaa attttccttc atatttagat tggaacgcag aaaatagaac
     5461 ttctaaagct atgaatcaaa ataaacttta cttttttatg aaaggctcat atttagtaaa
     5521 tgataggctc agaattcatg caagcagtat ttttgacaca aatccagttg caatctcaaa
     5581 caatgctaac aatgcattta cttcttttgg ctttgattat aaaataggcg aaaactctta
     5641 tttttctgca gaaattagca ttcatagaaa tcctttttta agacaatacg acccatttta
     5701 tttcgataat ccattagtaa acagaccttt cggcaattca ttgttttctg atccttttcc
     5761 acagtggtaa acaacacaac acttagcttt cctacttcaa aaaaagaaat gcaaacgctt
     5821 ggctggcaag ctgctgacat tattcttgtt agcggagatg cttttgttga ccacccatct
     5881 tttggcacag cagtaattgc acgagtttta gaaaaagaag gttttaaagt agctgtaatt
     5941 ccacaaccaa actggcaaga cgatttaaga gattttaaaa aacttggaaa accaaactta
     6001 ttttttggag ttagtgccgg aaatatggat tcaatgatta accactatac cgcagcaaaa
     6061 agaaaaagat ctgacgatgc ctacactgct ggaggaaaag ccggacaaag accagattat
     6121 gcaagcaatg tttattcaaa tattttaaaa cagctttatc ccgaaacacc agttattctc
     6181 ggtggtattg aagcctcaat gagaagattt gctcactacg atttttggca agataaaatt
     6241 ttaaactcaa ttttaattag ctcaaaagcc gacctattgg tctatggaat gggcgaacaa
     6301 tcaattagag aaattgcaaa acaacttaaa gaaacaaata atataaaatc ttgctacaac
     6361 ataaaacaat gtgcttacat aggcgaaatt gacgacatac aagattctga cattgagcta
     6421 ttttcttttc aagaagttgt aaaagacaaa ctaaaatatg cagcaaattt tgtgaaaatt
     6481 gaaaatgaga ccaattacaa tagcaacaaa agaattacac aaattacaga aaacaaaaaa
     6541 ataattgtaa atcctgcttt tccagcatta agcgaaaaag aaatggacga aatttacgat
     6601 ttgccttaca ctcgcttacc acatcaaaga tattcgaaaa aacaggcaat acctgcttac
     6661 gaaatgatta aaaattcggt aaatattcat cgtggttgct ttggagcttg tagtttttgc
     6721 actattgccg ctcatcaagg aaaaagtatt gcgagtcgtt caaaaaaatc aattttaaac
     6781 gaattacaaa aaatagcaga aatgcctgat tttaaaggac atataacaga cattggcggt
     6841 ccttcagcaa atatgtataa aatgcaaggt tttgatttga acatttgcaa aacatgtaaa
     6901 agaccttcat gtatatttcc aagcgtttgt aaaaacctta acacctctca cgaagaatta
     6961 ttaaatcttt atcaagatgc cttaaagatt aaaggtatta ccaaaatcag cataggtagt
     7021 ggcataagat atgatattgc aattcacgaa acaaagaatc ccaaaacaaa taaaatcaat
     7081 caaaaatatt tagaattatt aataagtaaa tttgtttctg gaaggcttaa agtagctcca
     7141 gaacactgct cgcctgaagt tcttaaacta atgagaaaaa cttcattctc aggttttaaa
     7201 aaacttagaa aaatattttt taacgtcaac gacaaatatg agttaaatca acagattatt
     7261 ccttatttta tttcatctca cccaggctgt aaagaagaac acatggcaga acttgcaaaa
     7321 gagttaaaat ccataggata taaaccagaa caagtacaat ccttcactcc cactccaatg
     7381 acattatcaa cagttatgta ttatactgga ataaatcctt acacaggcga aaaactttat
     7441 attgccaaat ctcaaaaaga aaaagacaaa caattaagct atttcttttg gtacaaaccc
     7501 gaaaatagaa aaagtaaaaa agaaagaacg aataaaaata ttagcatcaa aaaccgaaac
     7561 aaaactcgct aataatcaaa cacttagtaa agatttttta ttttttcaac acaaaacatc
     7621 ttaaaaaaac ataaaataat tcattaaaat ttaaaataat atttatatat ttgaaataaa
     7681 tatggttttt tatcggttat tttattaata tgaccgaaat tttttatgag aacttttaat
     7741 ttaaaaatat ttttcttatt attttgtcta acacttgtcg taaacgtgtt tagccaatct
     7801 actattcaac taaaattttc gcctattggg attcatccat ttaacgaaac taataaacat
     7861 ttatttgaca ataaaattga cgaaaatgcg atatttgtta cagagccttt atttttattg
     7921 tgctacgaat catatctaag agaagacagt tttgctttta gaggaatgtt tggcttttta
     7981 aacgatgctg ctgcaaaacc tgctattacc ttacatcttg gtcttaaaca aagattagtg
     8041 caaatctgga gaaactcatt cagtgttgga gctggcttta atttatatgg cagaggctta
     8101 tggtcaacaa ttccaggata tatccaagaa aaacattggg gggcaaacgg aacttgggaa
     8161 tataaacttg gctttatggg agagttagaa tatgcacttt ttttaaacga taaaaatgac
     8221 ttgacactat ctttactata tgggcatcaa caaaatactt ttacagttac aattggatat
     8281 aaatattgga tttcttcaat aataaaacat ccaaaaaaat gtggtaattg tccatttcaa
     8341 aagacaagta aacactggaa tccttaacta ttttaacaat ttaaaaaatt tgattatata
     8401 atggaaatta aacgagtttt tgacatttta gaattataca ataatgaata tgctgaatta
     8461 aaagacgcat tttcatacaa agaaaaaggt tcttgggtaa atttttcggc acaagactat
     8521 tataaatatt ctcatctttt tgcttgtgga ttaataaata aaggctttaa aaaaggtgaa
     8581 aaaataatta ctatgtctca aagtttaccg cattggaata ttgctgacat ggggatttcg
     8641 cttgctggat tagtaaacgt tcctatttac acaactcttg gtggagaaga attagaatat
     8701 attttaagac attctgatgc aaaaataatt atagtttctg accaaaatct ttataaaaaa
     8761 ttaaaaccta tagccgacaa aatcccagaa attcaaactg tttttacttt tcatcatatc
     8821 gaaggaatag aaaactgggt tagcttgatt caagaagcac aagaaaacga agaagagctg
     8881 atgaaaaaac ttgaagaaag aaaaaaatca gttgacgaaa atgatatgca cactctaatt
     8941 tacacttctg gaactacagg caatccaaaa ggtgtaatga tttcgcataa aaatatttta
     9001 agtaatgctt tagctgtaaa acctattgta ccaatagacc acggacaaag agcgttaagt
     9061 tttttaccac tttgccatat ttacgaaaga atgttaaatt attgcttcca aattaaagga
     9121 atttctattt attacgcaga aaatctcgga acaatagcta atgatttaaa agatattaaa
     9181 cctcatttat ttaattctgt accaagagtt ttagaaaaat tttacgatag aattatttcc
     9241 gtaggaaaag atttgaaagg aataaaaaaa tggatattct ttgcagcagt tagattagga
     9301 acgaaatacg atgacaaagg taaaaactct ttaatgtaca gattcaaatt aggtatttat
     9361 agaaaattag tattcaacaa atggaaagct gccttaggcg gaaacatagt agctgttgtt
     9421 tctggtggag catctttgca ggttaggtta gcaagattat atactgccgc aggagttaga
     9481 gtttgcgaag gttacggttt aactgaaaca tcgcctgtat tagcggtaaa ttggcccgac
     9541 tggaataaat caagacttgg aactgttgga cctattttag aaggagtgca agtaaaaatt
     9601 gctgaagata aagaaattct tgccaaagga cctaatatta tgcttggcta ttataaaaat
     9661 gaagaagaaa caaaaaaagt aattgacgaa gatggctggt tccatactgg agatattggg
     9721 gaatttatag ataataaata tctgaaaata actgatagga aaaaagaaac ttttaaaact
     9781 tcgagcggaa aatatattgc acctcaagta attgaaaaca aatttaaaac ttcaaactta
     9841 atagaacaat taatggttgt aggcgaaaac gaaaaatttg taagtgctct tatttcgccc
     9901 aatttcaact atcttcattt ttatgccagc aaacacaaaa tccactatcg cgaaaacagc
     9961 gacctaatta aacactccga aatcataaat aaaattcaaa aagaaataaa tttagttaat
    10021 gagacccttg gcgaacacga aagaattaaa agatttagac ttgtatgcga agaatggtcg
    10081 caacaaacag gcgaactttc accaactttg aagttgaaac gtaatgtgat ttataaaaaa
    10141 tacgctcaca ttcttgaaga aatttacgga cacggtgaag acaaaaccga aaatatgggc
    10201 aaagaaggtc caggtccaaa agaaactttt gaaaagaaaa tttctaatgg aatgaaaaaa
    10261 ttgataaata tttactcatc aaataaagaa taagccaaat cttggtaaag gctgtctcaa
    10321 aacgataaac ataaatgaac tctggtttta taaaggtaaa caataaaaat attttttata
    10381 aatttattaa ttctgaattt ttcgataata cagatgatgt tttaattttt ttacacgaag
    10441 gcttggggtc tataggtcaa tggaaagaat ttccgcaaaa attgtccgat aatttaaaaa
    10501 tacctgcttt agtttatgat agatatggat atggaaaatc agatggagta agcgaaagca
    10561 gaacactaaa ttatttggaa gaagatgccg aaaactttat gcctcagatt ttcaacaaac
    10621 ttctaaaacc aaattcaaaa aaaattttga tagggcatag cgatggcgga agtttagcta
    10681 ttttatatgc tgcaatgttt ccaaacgata cgaaagcaat tattaccgaa gcagctcact
    10741 tgtttgtaga agaaattact tataatggtg tgaagaaaat gtatgatatt ttttacgaaa
    10801 aaaacttaaa gaaaagattt gaaaaatatc atggcgaaaa tactgtttca atgtttcatg
    10861 gctggcatga tgtatggaca aacccaaact actttagttg gaatatagaa aaatatttaa
    10921 aacttattca atgccctgtg ctagcaattc aaggcgataa agacgaatac ggaactttaa
    10981 aacaattaga atccttggaa aacaatataa agtctccttg ccaaatcgag ataataaaag
    11041 actgcggaca tataccacat tttgaagcag agaaagaagt gaaaaatatt atgacaaatt
    11101 ttattatgaa cttgtaataa agacaaacat atttaaaaaa aatcacaaaa ttttcttaaa
    11161 acaataggtt ttttaatgat aaaatcctat atttgagttt tta
//
LOCUS       DAOPNX010000086        11033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_6786_length_11033_cov_5.929222, whole genome shotgun sequence.
ACCESSION   DAOPNX010000086 DAOPNX010000000
VERSION     DAOPNX010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11033
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6786_length_11033_cov_5.929222"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1317)
                     /locus_tag="PKN53_09005"
     CDS             complement(40..1317)
                     /locus_tag="PKN53_09005"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOD89126.1"
                     /translation="MLNRNIPPRTKKIKSINFCFPEKHSFQNGINTYVLGSETAPVIK
                     LDIIFKSGIENAEKKLEPTFANSMLKEAPQGMTSNETADFFDYYGAYIQNFISNNTSG
                     LRLFVPQRFFVKVLPVFADLIQNPAFIEEDFSILKEKSKESIKVNLTKTKYIAAKGIN
                     NQLFGDFHPRGWLVKPEDVDTINLKDVKKFSNTYFSSKNCFIIASGSVNTETIKLIEK
                     HFSKEFGNNKFVKKPTNKRIFSEKEIFKHYEMPNAVQSSIYLAKNLGKLRDEEIVDIG
                     ILNTVLGGYFGSRLMKNIRENKGYTYGIGSFLTDYDDCTVLKISSELATKYVKKTIKE
                     IFKEIEKLTTTPISNSELNLVKNYSSGEILSSIDGVFQNSIIWEKIISSQRQENLINL
                     QFERLQNITSDELMQIAQKHLNKEEFFTAIAGS"
     gene            complement(1317..2561)
                     /locus_tag="PKN53_09010"
     CDS             complement(1317..2561)
                     /locus_tag="PKN53_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOD89127.1"
                     /translation="MIKFEKFILNNGLRVIVHKDDTTPLVAFNLLYDVGAKDENPNQT
                     GFAHLFEHLMFGGSINVKNYDKVLQKASGENNAFTNNDYTNYYITLPKQNLETAFYLE
                     SDRMLSLAFSQNSLDVQKNVVCEEFKQRYLNQPYGDALAEIRSLAYKKHPYQWQTIGK
                     ELSHITNASLDDVKSFFYKFYAPNNAILSIAGDVNLIEIQKLCEKWFGDIPKRNVPKR
                     NLPKEPEQKEKRSKEIIKDVPSNALYMAYHTGGRRDDDYHATDLISDLLANGPSSLFN
                     KILIKEKKLFPQIDAYIQGSIDPGLLNINARLFDTTSYEVAENAIKTEINKITNGDFS
                     EKELTKVKNKSISNMLFSNIGILDIAMELAYFELLGNAELINSVEDNILEVSKSDIIK
                     TAQKIFKEENLSCLYYKSKNLI"
     gene            2757..7262
                     /locus_tag="PKN53_09015"
     CDS             2757..7262
                     /locus_tag="PKN53_09015"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOD89128.1"
                     /translation="MKKLYLFVVLFLSSFLLYSQKSYDASFNQKSSDVYEITFTVQDF
                     DLNEVNIDGNIFTKIDMGVSVSSQKQGWAELPFISASIALPAQKDVDLNITYTNYVDI
                     PLDYPLLPSRGVIYRNQDPSQIPYVIAPESIIDEFYPSENVVMEDPFIFREVRGTTVR
                     VFPFQYNAVSQTLRFYNNIKVELVENNDEPTNPFLKERASYIREVEEIYKDIFLNYND
                     QTKVSADLTSADYGDILVITTSAYSATMDTYIQWKKEMGYNVTKTIVSQGTNVANTIS
                     TAYNNNPNLMYVQLAGDWADIKSPTISAGYFEPPAPCNPFMGAVSGSDNYIDISVGRF
                     SCASTSDLLVQINKAINYEKNPNNDAGWRETFIGVSSDEGPGDDNEYDYTHVQRIYTQ
                     RLQPFTYNTHKQNYDPGASATTLAGHLNAGASTLAYCGHGSETSFVSTGFSNTHINNL
                     TNGDKLPFIVSVACVNGTFHYSEDCFAEAWLRKANGGAVVTLMSTINQPWTPPQRGQD
                     YFYDILIGGFNYDTDGIPQTTGINTSEQRTHWGSIVVNSSVLMLKESSASDDIETIKT
                     WTTFGDASLQLRTKQPETLTLTNMAPEVGVPFTGVAKINGTPAENVMICISANDNYYS
                     TLTNSSGQYTIDHSLVAGEALLVATAFNSTTIYQNVQVIDADPCFPVTNLSATTSTGS
                     VTLTWTAPTEGNVTGYKIYRDGSLQTTVTGLTYTQSSVSTGYYNYCVAAVYDGTECYA
                     QECVNVFVNDGTNTSCEAPTNLNVEEQSPTVHNLTWTAPLGSNVIFDDIESHTAFTIN
                     SAGSVPWTFIDGDNQATYSIANYTFQNQGSQMATIVFDPNLVTNNNDGTPLTQTTDGT
                     PFTAHSGNQFFACFSATSGQTNDWIISPELNFDESFTFKFFARSGHKTQYAESFRVAY
                     STTTNTAAAFTNILATVNSAPFEWTEYSYNVPANAKYVAINCNSTDKYYFCVDDIYIG
                     DGSMPTAAPIAYNVYCDGALIGTSTTTDFSYTEATSGSHQYCVEAIYADDCVSPQVCA
                     NIGTVINTYTITATAGNGGSISPSGSVEVNEGENKTFTITPNTNYSISDVAVDGISVG
                     AVNTYTFSNVNSNHTISATFNYSCLAVNNLDATSEAGSITLTWTAPASGNVTGYKIYR
                     DGSLQATVTALTYTQSGLSGGSYEYCVATVYDGVECTNTACATVVVLNNYTITASASN
                     GGSISPEGSVAVVEGNDKTFTITPDDCYVLADVIVDGISVGAVSTYTFSNVNSNHTIS
                     ASFNQTTYAVNSSTSAGGSITLASSIGCGDDLTINILPDECYELTELTINGEIQTVVG
                     NTFVYENVQSDLEIYANFSEKIVIITASANEGGSITPSGEVEVNCGENQLFVISANEG
                     YYIKNVYVNGENQGAPESYLFSNITEDASIEVEFALISDIMLANNTSEIEIFPNPCVD
                     KFNMLIPEVELYNTCEIYSNDGKLIRSFKITELNNELEISEFASGTYYIKVYGNEKVD
                     VIKLIKY"
     gene            7600..9846
                     /locus_tag="PKN53_09020"
     CDS             7600..9846
                     /locus_tag="PKN53_09020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD89129.1"
                     /translation="MFSFSLNAQICDPPCTPDQSCIANSVQAGYLCSGSNEPMVEIAD
                     VYYEETITFRPPEGDYYNPYAAIRLDAITNLPDGMTWCKSQDIFYVTEPTTYYCVQLK
                     GTPTTPGRYVLSLTTTLFADNNGTLQPLEQYTDTPLVVIVLPSDVEVPVADFIWTGGQ
                     CRGDTIVFTDISPVGSNCVWKVDGYVVNEGSSIFSYVFPPVYSPTNFSVSLSVSTEYL
                     TSETTQNITIYPPPFVNISYSNDVVCHGESVTLTASGAETYLWSNEETSAEITVTMYE
                     STVFTVIGTDINSCVNKDSLTVYVMPNDTIIVYDEFCEGDVYEFYGNNFTEEGIYYID
                     IISPSTNCEGVVQLELTVKPKPQQVVVLQDPENGILPLGTSGSITLETSEVGIGYFVT
                     IGGEPITDLISGTGSSLSLGDNYPAGSYDVMSYNQYDCYLVQGTVNFTTPASSNTGKI
                     VANVSFGTPPSSFPTNHVKVKLYKAAIDIANNEVIVLMDQQLLDANGQAVFAGLVSGD
                     YYLGSFVQYPENYNVAEHVYYQDAVVHEDAISIPIEEGTVFFASLHHVLLTESQGSNT
                     MQGIVGASSNNSKGLNPIEDMVVILKNSDINEIIGVSVTSDNGQYYFDNIPDNANIQA
                     FVTNLSCQNYIAFETQTTSDQIYNVNFIVTETSIYPNDITMLENVQIHNIEFRVFPNP
                     AKEVLNINCNVEKAILKIFDINGRLIYTDLAFPNIEINISELVSGTYIIILSAENGKT
                     GVQKFVKE"
     gene            complement(9922..10371)
                     /gene="rplI"
                     /locus_tag="PKN53_09025"
     CDS             complement(9922..10371)
                     /gene="rplI"
                     /locus_tag="PKN53_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOD89130.1"
                     /translation="MEIILKQDVDNLGHKDEIVNVKPGYARNYLIPQGYAIMATASAK
                     KVHEENMKQKAHKEAKLREDAEAVAEKMNGLSLRIGTKTSSTGKIFGSINTVMIAEAL
                     EKEGFNIERKNITLKGEAIKEVGNYEANVKIYKNIKVVVPFEVYSEE"
     gene            complement(10389..10652)
                     /gene="rpsR"
                     /locus_tag="PKN53_09030"
     CDS             complement(10389..10652)
                     /gene="rpsR"
                     /locus_tag="PKN53_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOD89131.1"
                     /translation="MAQNDIRYLTPLAVEVKKKKYCRFLRNRIKYVDYKDPQFLKRFL
                     NEQGKILPRRITGTSLKFQRKVAQSVKRARHLALLPYVTDMMK"
     gene            complement(10655..11026)
                     /gene="rpsF"
                     /locus_tag="PKN53_09035"
     CDS             complement(10655..11026)
                     /gene="rpsF"
                     /locus_tag="PKN53_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOD89132.1"
                     /translation="MNQYETVFIATPVLSEAQMKEAVNKFRRIITDNNGELIHEEDWG
                     LKKLAYPIKKKSTGFYYLFEFRSEGEFINKLETEFRRDERIMRFLTFKMDKHAIAYSE
                     KKRKMNSKTADNKNEQNQKEA"
BASE COUNT         3616 a         1625 c         1818 g         3974 t
ORIGIN      
        1 taagcgatgc actgaacgta gttgaagtgt taaaagtgtt caactgcctg caatggcagt
       61 aaaaaattct tctttgttaa ggtgtttttg agcaatttgc attagttcat cgctagtgat
      121 attttgtaat ctttcgaact gtaaattaat taaattttct tggcgttgtg agctaataat
      181 tttttcccaa attattgaat tttgaaaaac tccatctata gatgatagaa tttcaccact
      241 gctataattt ttaactaaat ttaattctga attagaaatt ggcgtggttg ttagtttttc
      301 aatttcttta aaaatttctt ttattgtttt tttaacatat tttgttgcaa gttcgctact
      361 tatttttaaa acagtacaat cgtcataatc tgttaaaaat gagccaattc cataagtgta
      421 gcctttgttc tctcttatgt ttttcatcaa tcttgaacca aaatacccac ctagtactgt
      481 gtttaaaatt ccaatatcaa caatttcttc atctcttagt ttgccaaggt tttttgctaa
      541 ataaatactt gattgaacag catttggcat ttcgtaatgt ttgaaaattt ctttttctga
      601 aaaaatcctc ttgtttgttg gttttttaac aaatttatta ttgccaaatt cttttgagaa
      661 atgtttttca attagtttaa ttgtttctgt attcacactt ccagaagcaa taataaagca
      721 atttttagat gagaaatatg tgttggaaaa ttttttcaca tcttttaaat ttatggtatc
      781 aacatcttct ggcttaacca gccagcctct ggggtgaaaa tctccaaaaa gttgattatt
      841 tattccttta gcagctatat attttgtttt tgttaaattg acttttattg attctttact
      901 tttttctttt aagattgaaa aatcttcttc aataaaagca ggattttgta ttaagtcagc
      961 aaaaacaggt aaaactttta caaaaaatct ttgaggaaca aatagtcgta agccagaagt
     1021 attgttagaa ataaaatttt gaatgtaagc accgtaataa tcaaaaaaat cggctgtttc
     1081 gttgctagtc attccttgtg gagcttcttt tagcattgag ttagcaaaag ttggttcaag
     1141 ttttttttca gcattttcaa ttccagattt aaaaataata tctaacttta ttactggagc
     1201 tgtttccgaa cccaaaacat aagtgttaat tccattttgg aagctgtgct tttcagggaa
     1261 acaaaagttt attgatttta ttttttttgt gcgtggtgga atatttctat ttagcattaa
     1321 attaagtttt tagatttgta atacaaacaa gataaatttt cttctttaaa aattttttga
     1381 gcggttttta taatatcaga ttttgaaact tccaaaatat tgtcttcaac agaattgatt
     1441 aattcagcat ttccaagaag ctcgaaatat gctaattcca tagcaatgtc taaaattcct
     1501 atattagaaa aaagcatgtt tgaaatagat ttgtttttta cttttgtaag ttctttttcg
     1561 ctaaaatcgc cattagtaat tttattaatt tctgttttaa tagcattttc tgcaacttca
     1621 taacttgtag tgtcaaaaag tctagcattt atatttagca atcctggatc tatgctacct
     1681 tgaatatatg cgtcaatttg tgggaaaagt tttttttctt taataagaat tttgttaaaa
     1741 agcgacgaag gtccatttgc taataaatct gaaattaagt cagttgcatg ataatcgtca
     1801 tctcttcttc cgccagtgtg atacgccata tataaagcat ttgaaggaac atctttaatt
     1861 atttctttac ttcttttttc tttttgctca ggctcttttg gcaagtttcg ttttggtaca
     1921 tttctttttg gaatatcgcc aaaccatttt tcgcaaagct tttgaatttc tattaaattt
     1981 acatctccag caattgataa tatcgcattg ttaggtgcat aaaatttata gaaaaaactt
     2041 ttcacatcgt ctaaacttgc atttgttata tggcttaatt cttttccaat agtttgccat
     2101 tgataagggt gttttttata agctaaactt ctaatttcag caagagcatc tccataaggt
     2161 tggtttaaat atctttgttt aaattcttca caaacaacat ttttttgaac atcaagacta
     2221 ttttgcgaaa atgccaaact caacattcta tctgattcta aataaaaagc agtttcaaga
     2281 ttttgtttag gaagagttat ataataattt gtgtaatcat tatttgtaaa agcattgttt
     2341 tctccacttg ccttttgtaa aactttgtca tagtttttaa catttataga acctccaaac
     2401 atcaaatgtt caaacaagtg agcaaaacca gtttgatttg gattttcatc tttagcacca
     2461 acatcgtata aaagattgaa agcaactaaa ggggttgtgt cgtctttgtg gactattact
     2521 cttaatccgt tgttcaaaat gaatttttca aattttatca tgtgcaaaaa ataatatttt
     2581 caaatatata actaatttgt tttaaaattg ttgttttgtc gttttaaaat gggtgttagt
     2641 aaaataatca aaaaaaaatg ggatattttg caataattag ttaactttgt gtcatgtgaa
     2701 atttaattat acgtattaat ttatttaata gttgtcaaat ttttaaatcc tatattatga
     2761 aaaaacttta tttatttgtt gtattgtttt tgagttcatt tcttttatat tctcaaaaat
     2821 cttatgatgc aagtttcaat cagaaatcat cagatgttta tgaaattaca tttaccgtac
     2881 aagattttga tttaaacgaa gtaaacattg acggtaatat ttttacaaaa atcgatatgg
     2941 gtgtaagcgt atcttcacaa aagcaaggtt gggctgaact gccttttatt agtgcttcta
     3001 ttgcattacc tgctcaaaaa gatgttgatt tgaatattac ttatacaaat tatgttgata
     3061 ttccattgga ttatccactc ttgccttcaa gaggagtgat ttatcgtaat caagatccat
     3121 cgcaaattcc ttatgtgatt gcaccagaat ctattataga tgaattttat ccaagtgaaa
     3181 atgttgtcat ggaagatcca tttattttta gagaagtaag aggaacaact gttagagtat
     3241 ttccatttca atataatgct gttagccaaa ctttgagatt ttataataat attaaagttg
     3301 aattggttga aaataatgac gagcctacaa atccattttt aaaagaaaga gcaagctata
     3361 tacgtgaggt tgaagaaatt tataaagata tatttttgaa ttacaatgat caaactaaag
     3421 tttctgcaga tcttacttct gctgattatg gtgatatttt agtaattacc acttctgctt
     3481 attcagctac catggatact tacattcaat ggaaaaaaga aatgggttat aatgtaacta
     3541 aaacaattgt aagtcaaggg actaatgttg caaatacaat ttctactgca tataacaaca
     3601 accctaattt gatgtatgtt cagttggctg gagactgggc agatattaaa tctccaacaa
     3661 ttagtgctgg atattttgag cctccagctc cttgtaatcc atttatgggg gcagtttcag
     3721 gtagtgataa ttatatagat atatctgtag gaagattttc ttgtgcaagt actagtgatt
     3781 tattggttca aataaataaa gcaattaatt atgagaaaaa tcctaataat gatgctggct
     3841 ggcgtgaaac ttttatagga gtatcttctg atgaaggacc tggagatgat aatgaatatg
     3901 actatactca tgttcaaaga atttatactc aacgtttaca accatttaca tacaatacac
     3961 ataaacaaaa ttatgaccca ggtgcatctg ctactacttt agctggacat cttaacgcag
     4021 gagcatcaac attagcttac tgtggacatg gttcagaaac ttcttttgta agtactggat
     4081 ttagcaatac acatattaat aatttaacaa atggagataa acttccattt attgtttcag
     4141 tagcttgtgt taatggtact ttccattata gtgaagattg ctttgcagaa gcttggttac
     4201 gcaaagctaa tggtggagca gttgttactt tgatgtcaac aataaatcag ccatggacac
     4261 caccacaaag aggtcaagat tatttttatg atattttgat aggaggtttt aattatgata
     4321 ctgatggaat tccgcaaact actggaataa acacctcaga acaacgcaca cattggggtt
     4381 caattgttgt aaattcttct gttttaatgt taaaagaaag ttctgctagt gacgatatag
     4441 aaactattaa aacttggaca acttttggtg atgcttcatt acaattaaga acaaaacaac
     4501 ctgaaactct tacattaaca aatatggcac cagaagttgg agtgcctttt acaggagttg
     4561 caaaaatcaa tggaacgcct gctgaaaatg tgatgatctg tatttcggca aacgacaatt
     4621 attactcaac tttaacaaat agttcaggac aatatacaat tgatcatagc cttgtagcag
     4681 gagaagcttt gttagttgca acagctttta attctactac aatttatcaa aatgtacaag
     4741 ttatagatgc tgacccatgt tttccagtaa caaatttgag tgcaacaact tcaacaggtt
     4801 ctgttactct tacttggact gcaccaaccg aaggaaatgt tacaggatat aaaatatata
     4861 gagatggaag tttgcaaaca acagttacag gtttaactta tactcaatct agcgtttcta
     4921 cagggtatta caactactgt gttgcagcag tttatgatgg aactgagtgt tatgctcaag
     4981 aatgtgtaaa cgtttttgta aatgatggta caaatactag ttgcgaagct cctactaact
     5041 taaatgttga agagcaaagc ccaacagttc ataacttaac atggacagct cctttgggtt
     5101 caaatgtgat ttttgatgat atagaatcgc atactgcctt tacaataaat agtgcaggtt
     5161 cagtgccttg gacttttatt gatggtgata atcaagcaac ttattcaatt gctaattata
     5221 cttttcaaaa tcaaggctca caaatggcta caatagtttt tgacccaaat ctggttacaa
     5281 ataataatga tggaactcca ctaactcaaa caactgatgg cacaccattt actgcacatt
     5341 cgggcaatca gttttttgcg tgttttagtg caactagtgg acaaacaaac gattggatta
     5401 ttagcccaga attaaatttc gatgaatcat ttacatttaa attctttgct cgcagtggac
     5461 ataaaacgca atatgcagaa tcatttagag ttgcttattc tacaactaca aatacagcag
     5521 cggcgtttac taatatttta gcaactgtaa actcagctcc ttttgaatgg acagaatatt
     5581 cttataatgt tccagcaaat gcaaaatatg ttgctataaa ttgtaattca actgataaat
     5641 attatttctg tgttgatgat atttatattg gcgatggttc aatgccaacc gctgcaccaa
     5701 ttgcttataa tgtttattgc gatggagcat taataggaac aagcacaact acagattttt
     5761 catatacaga agcaactagc ggttcacatc aatattgtgt tgaagcaatt tatgctgatg
     5821 attgtgtttc gcctcaagtt tgtgcaaata taggaactgt tataaatact tatacaatta
     5881 ctgcaacagc tggcaatggc ggaagcataa gcccttcagg aagtgtagaa gttaacgaag
     5941 gcgaaaataa aacttttacc attacaccaa atactaatta ctcaatttct gatgttgctg
     6001 ttgacggaat ttctgttgga gctgtaaata cttatacatt tagcaatgta aattcaaatc
     6061 atacaatttc tgcaacattt aactatagct gtttggctgt aaacaattta gatgcaacaa
     6121 gtgaagcggg ttctattact cttacttgga ctgcaccagc ttcaggaaat gttacaggat
     6181 ataaaattta tagagatgga agtttacagg caacagtaac agccttaaca tatacacaat
     6241 caggacttag cggtggttct tacgaatatt gcgttgctac tgtttatgat ggtgtagagt
     6301 gtactaatac agcatgtgca actgttgttg tattaaataa ctatacaatt acagcatcag
     6361 cttcaaatgg tggaagtata agcccagaag gaagtgtagc tgtagttgaa ggaaatgata
     6421 aaacatttac aataacacct gatgattgtt atgtactggc agacgttatt gttgatggaa
     6481 tctctgttgg agctgtaagt acttatacat ttagcaatgt aaattcaaat catacaattt
     6541 ctgcttcgtt taatcaaaca acttatgccg ttaattcaag tacaagtgca ggaggcagca
     6601 ttacgcttgc ttctagtatt ggttgtggcg atgatttaac tataaatatt ttaccagacg
     6661 aatgttatga attaactgaa ttaacaataa atggtgaaat acaaactgtt gtaggcaata
     6721 cttttgttta tgaaaatgta caatcagatt tagaaatata tgcaaatttt tcagaaaaaa
     6781 ttgttataat cacagctagt gcaaatgagg gcggaagtat tactccttct ggtgaagttg
     6841 aagttaactg tggtgaaaat cagctatttg ttatatctgc aaatgaagga tattatatta
     6901 aaaatgttta tgttaatggc gaaaatcaag gtgcgccaga aagttattta ttctctaata
     6961 ttaccgaaga tgctagcata gaagttgagt tcgcacttat ttctgatatt atgcttgcaa
     7021 acaatacaag tgaaattgaa attttcccaa atccttgtgt tgataagttt aatatgctaa
     7081 ttccagaagt agaactttat aatacttgcg aaatatattc taatgatgga aaactaatta
     7141 gaagttttaa aatcacagaa ttgaataatg agttagaaat ttcagaattt gcaagtggaa
     7201 cttattacat aaaagtttat ggaaatgaaa aagttgatgt gataaagttg ataaagtatt
     7261 agtttagttt ataaatatgg aaattggcta tcaatttagg tagccatttt tttatacggt
     7321 gatttgtatt gataagaatt taactagttt ataaagaaag ttcataagtt gaataattgt
     7381 ttaaaacgtt tatcgtgatt ttttttataa aattatctct attattaaaa aaagttgtat
     7441 atttgtagag agttataaag gggatttttt aagtgttgga tataaatttg tataattcga
     7501 ttaaataatt gcttggttat cagtggatta aagtataata aattaacaat taaaataaat
     7561 gaacttacga aacgtctaat ttttactttt tcaattttgg tgttttcctt ttctttaaat
     7621 gctcaaattt gcgaccctcc ttgtactcca gaccaatctt gtattgcaaa tagtgtacaa
     7681 gccggatact tgtgttcagg ctctaatgag cctatggttg aaatagcaga tgtgtattat
     7741 gaagaaacca taacctttag accgcctgaa ggcgattatt acaatccata cgcagctata
     7801 cgacttgatg caatcactaa tttgcccgat ggaatgactt ggtgtaaaag tcaagacata
     7861 ttttatgtta ctgagcctac tacttactat tgtgttcaat taaagggaac acccacaaca
     7921 ccaggtcgtt atgtcttatc attgacaact accctttttg ctgacaataa tggcacttta
     7981 caacctctcg agcaatatac agatacaccc ttggtagtaa tagttctgcc atctgatgtt
     8041 gaagttcctg tagcggattt tatatggact ggggggcaat gtaggggcga tactatagtt
     8101 tttactgata ttagtcctgt agggagcaat tgcgtatgga aagttgacgg atatgtagta
     8161 aatgaaggaa gttccatttt ttcctatgtt ttcccaccag tttattctcc aacaaatttt
     8221 tcagtaagtt tgtctgtatc tacagaatat ttaacatcag aaactacaca aaatattacc
     8281 atatatccgc ctccttttgt taatatatct tactcaaatg atgtcgtctg tcacggagaa
     8341 tctgtaacac taactgctag tggagcagaa acttatttgt ggtcaaatga agagactagt
     8401 gccgagataa ctgttaccat gtatgaatct actgtattta ctgttatagg tacagacatt
     8461 aattcttgtg taaataaaga tagtcttact gtgtatgtaa tgccaaatga tactatcata
     8521 gtttatgatg aattttgcga gggtgatgta tatgaatttt atggaaataa ctttacagaa
     8581 gaaggaatat attatattga cataatatcg cctagtacta attgtgaggg tgtggttcag
     8641 cttgaactaa ctgttaaacc taaacctcaa caagttgtag ttttacaaga tccagaaaat
     8701 ggaattttac ccttaggcac aagtggtagc ataacactag aaactagtga agttggaata
     8761 ggttattttg ttactatagg tggagaacct attactgatt taattagtgg aacaggctca
     8821 agtctaagtt taggagataa ttatccagct ggaagttatg atgtgatgag ctataatcag
     8881 tatgattgtt atttagtgca aggaacggtt aattttacaa ctccagcaag tagtaataca
     8941 ggaaaaatag ttgctaatgt gagttttggt acgcctccaa gtagttttcc aaccaatcac
     9001 gtaaaagtaa aattatacaa agcagcaata gatattgcca ataacgaagt tatagtttta
     9061 atggaccagc aattattaga cgctaatgga caagctgtgt ttgcgggttt agtatccggc
     9121 gattattatt taggatcatt tgttcaatat cctgaaaatt ataacgtagc agaacatgtt
     9181 tactatcaag atgctgttgt gcatgaagat gctataagta ttccaataga agaaggaaca
     9241 gtgttttttg ctagcttgca tcatgtttta ttaacagaaa gtcaaggttc aaacacaatg
     9301 caaggtattg tcggagctag tagtaacaac agtaagggtt taaatccaat tgaagatatg
     9361 gttgttatac taaaaaatag tgatataaat gaaattattg gagtaagcgt tactagtgat
     9421 aatggtcaat attactttga taatattcca gacaatgcta atatacaggc ttttgtaacc
     9481 aatctttcat gtcaaaatta tatagcattt gaaacgcaaa ctactagcga ccaaatttat
     9541 aatgttaact ttattgttac cgaaacaagc atttatccaa atgatattac aatgcttgaa
     9601 aatgtacaaa ttcataatat tgaatttaga gttttcccaa atcctgcaaa agaagtatta
     9661 aacataaatt gtaatgttga aaaagctatt ttgaaaattt ttgatataaa tggaagactt
     9721 atttacacag atttagcttt cccaaatatt gaaataaata tttctgaatt agtttcagga
     9781 acttatataa ttattttatc agcagaaaat ggtaaaactg gggttcaaaa atttgtgaaa
     9841 gaataaacat tatagaaatt attaataaaa aaaaggcgaa acttttgatg tttcgccttt
     9901 ttttatagat acattatttt tttattcttc tgagtaaact tcaaaaggaa caacaacttt
     9961 aatgtttttg taaattttta catttgcttc gtaatttcca acttctttaa tggcttcgcc
    10021 ttttaaagtt atatttttac gttcaatgtt aaagccttct ttttcaagag cttcggcaat
    10081 cataacagtg ttgattgaac caaaaatttt gccagtagag ctggtttttg ttccaattct
    10141 taatgataaa ccgttcattt tttctgcaac agcttcagca tcttcgcgaa gtttagcttc
    10201 tttatgtgct ttttgtttca tgttttcttc atgaactttt ttagcagaag cggtagccat
    10261 aattgcataa ccttgaggta taagataatt tctggcatat ccaggtttta cattaactat
    10321 ttcgtcttta tgtcctagat tatcaacgtc ttgttttaat ataatttcca tttcttgtcc
    10381 tccaaatttt atttcatcat atctgtaacg taaggtagta atgctaaatg tcttgctctt
    10441 tttacagact gtgcaacttt acgttgaaat tttaatgatg ttccggttat tctacgtggt
    10501 aaaatttttc cttgttcgtt taaaaatctt ttcaaaaatt gtggatcttt ataatctaca
    10561 tattttatgc gatttcttaa aaaacggcag tattttttct ttttaacttc aactgctagt
    10621 ggtgtcaaat atctaatatc attttgtgcc ataattacgc ctccttttga ttttgttcgt
    10681 ttttgttatc agcagttttg ctgttcattt ttcttttttt ctcactatag gcaatagcgt
    10741 gtttgtccat tttaaaggtt aaaaaacgca taattctttc atcacgtcta aactctgttt
    10801 caagtttgtt gataaattca ccttcagatc tgaattcaaa caagtaataa aaacctgttg
    10861 actttttctt gattgggtaa gcaagctttt tcaagcccca atcttcttca tgaattaatt
    10921 caccgttgtt gtcggtaata attcttctaa atttgttaac cgcttccttc atctgtgctt
    10981 cagacaaaac gggagttgca atgaaaacgg tttcgtactg attaatcata att
//
LOCUS       DAOPNX010000087        10732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_7011_length_10732_cov_7.030814, whole genome shotgun sequence.
ACCESSION   DAOPNX010000087 DAOPNX010000000
VERSION     DAOPNX010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10732
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7011_length_10732_cov_7.030814"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..838
                     /locus_tag="PKN53_09040"
     CDS             77..838
                     /locus_tag="PKN53_09040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOD89133.1"
                     /translation="MSLNTAFAQNDSIYVMKDGNILWQYKVTEIDSIIFYKPVIEEET
                     SGTFVDERDNREYSWVKIGEQVWMAENLKYLPSVVGPGTGSNTEAYYYVYDYDGTNVD
                     AAKATENYATYGVLYNWTAAMAGAVSSDANPSGVQGICPDGWHLPSDAEWTQLKDYLG
                     ENAGGKLKEVGTTHWADPNTGATNESGFTALPGGGRSHEGNFYDVGNLGVWWFSTEYE
                     SDKAWGWYLGNSNSGIFSGYGYRNEDYSIRCVRDN"
     gene            complement(957..1451)
                     /locus_tag="PKN53_09045"
     CDS             complement(957..1451)
                     /locus_tag="PKN53_09045"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HOD89134.1"
                     /translation="MIQRIQTLYLFVSFICFGIFFFSPFGYLITPELQEIPIKITESN
                     FSYHNNALNQDIVLEYSMLPLLILVSLVCFISFFTIFLYKKRMLQVRLTVFNILLQIG
                     CFGLLFYYLIDISKRLELEYSTGILIILPLISVILCFLAMRAILKDEALVQASKHFRK
                     SVKS"
     gene            1560..2525
                     /locus_tag="PKN53_09050"
     CDS             1560..2525
                     /locus_tag="PKN53_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD89135.1"
                     /translation="MNYKDNVEAINALRQKYESLISEITKKIYGQDQVVKEVVISIFS
                     RGHCLLVGVPGLAKTLLVNTIAEVLGLKNKRIQFTPDLMPSDIIGTEILTEDRQFKFV
                     KGPIFANFILADEINRTPPKTQAALLEAMQERSVTAAGQEYKLDEPFFVMATQNPIEQ
                     EGTYPLPEAQLDRFMFNIILDYPAYEDEITVVKNTTVDKEFELKQVLTAEEIIFFQGL
                     IRKIPINDNVLEYAVKLACKTRPNSEFAPEITNKYLHWGAGPRASQYLVIGAKTHAVL
                     NGKYTPDIEDVKAVAKIILRHRIIKNYKAEADGISIDDIIDELLK"
     gene            2529..4079
                     /locus_tag="PKN53_09055"
     CDS             2529..4079
                     /locus_tag="PKN53_09055"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HOD89136.1"
                     /translation="MLRNISKIACLVFCFLFFAILTNAQDKKDEKPRVVEILRASSLE
                     YDESTGVTAKRLLGDVVFRHEDAYMFCDSAWFFDDVNTIKAYNNVRIKQGDSILMVGK
                     YLEYDGNTKIAKMRDSVILKHNKSFLITDSLDYDRNIDVAYYFEGGKIYDEDNRLFSR
                     RGYYYTKDKDYYAVDTVVLKNPQYDIFSDTLKYNTESTIAYFFGPTNIVSDSNHLYCE
                     LGYYDTSEDKASLSNNAWLRSGQNYLLGDSLFYDRKIRYGEAFMNVSVVDTIENLVAK
                     GNYGYYYEEPQNAMLTKNTEVIYVSDGDSIFMHSDTVYISVDTADFKLIRAFYKVQVF
                     KSDMQARCDSLVFYSYDSIAHMFHNPVLWAENSQITANHIEMHFLDKKPDKFYLNNNA
                     FAIEQCDSVHFNQLKSRKMSGYIVEKKVRQIDLHNDCQTIYFIVDEEVDEIVALNKVV
                     STNMRIWLKDNKVENVWFFDKPDGETIPIEQLKDEQTFLKDFRFLDEYRPKSREDIFI
                     WKEIPQNE"
     gene            4142..4651
                     /locus_tag="PKN53_09060"
     CDS             4142..4651
                     /locus_tag="PKN53_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89137.1"
                     /translation="MEKNKLLIAVLGNEGSGKTTTWETLFGRKVHTGKNLRNLVIQGI
                     KIPVFLINESPLERKTKLEYILPDDDPEIVISSFLYHKDVVENFEYFINRGYNIYVQW
                     LNPGYNDKNHKALFYNDGIINYLLRCGAIVSAKNAKNNPVSRVNEIKNYLLSYFLTKN
                     NLNVSIKSQ"
     gene            4773..5036
                     /locus_tag="PKN53_09065"
     CDS             4773..5036
                     /locus_tag="PKN53_09065"
                     /inference="COORDINATES: protein motif:HMM:NF016855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family DNA transformation protein"
                     /protein_id="HOD89138.1"
                     /translation="MGIVGAHNIGKVLELKLNEVGITTLKELKEMGTEKTFLLLYEKN
                     KNTTRATLLSIEGAIQEIRWHNLDEKRVAELREFYDSVVEKRK"
     gene            5124..8354
                     /locus_tag="PKN53_09070"
     CDS             5124..8354
                     /locus_tag="PKN53_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD89139.1"
                     /translation="MQKIEKHPILKVPEKNEHLFYFNDKAVVGTKGFTIAAALHQAGF
                     PVHSHSLDNRKRSLECGIGKCGACEMLVDGKIKRICITKVDGIREVREIPKDFEPSQE
                     KINLKDEISIFKTQAVIIGAGPAGLAAREVLNQHEVSNIVVDNNDKIGGQFLMQTHQF
                     FFFEKEKKFGGLRGFEIAETLAGDNREGILLNSVVWDILEGPRLAVKNITTNEIFYID
                     AEYIIIATGAIPFMPSFKNDDVPGVYTAAVVQKMMNNEFTLLGKNILTVGAGNIGYLT
                     SYQLMQAGAKVKAIIEAQPNEGGFPVQANRLRRLGIPIMTSHILIEAIPNEDYTGITG
                     AVIAECENFKLIPGTEKIITGIDAINICTGLVPDNQLVTKANLLFGRKSFAIGDALRI
                     GEGTSAVLRGKQAAYELIDALGKRYNYTDYLSVSKEYIESQQHPTRIIQEPNKPTRER
                     AEAKPYVIMDCLYGFACNPCTFSCKQGAITKLSTSTVPVIDYDKCIGCMDCVYQCPGL
                     AIFGYSEKNNWLFLPIEYEVEENSEVYLVNNNGEKIGEGIIEKILYKPNKTNVARVKA
                     LDLQGEQMQAARGFIVKKDYPQPLEFKNVKGESQAETYICHCDDVKLEDILKIIGDRK
                     FISIDEIKHITRLGMGACRGKRCIKRLKQTISAYGIEIVGDATPRAPLSNQVSMGELY
                     PHESERIFTNVNKKSNKRIKVQALVAGGGMAGSSLFKFMAEAGMKPVLVNRERGSSWR
                     NIAGGRPAFSLPALADIALKNRELFIELQNICDIDYVPTKYVSFAHDNETYKALEASK
                     SWSDAFMVEKVDFRKEISPYFNNNLDTYSAALITNDCWQATPGKVLDLLRKIGIEKGG
                     QVLEDCELIDLQREGKNVIALVKTHTGEFVEYETEHFVNALGNESSKFAKKLGLEIGL
                     YPVKHQAFITRRLPLLGKDGKTLDMLIDRRQYKGFSAVYGQQLATTGQIIGCASPLID
                     ALETDKEVKTNAKDFLEIVSEVFVDWIPQLSSVGFQAAWAGYYIEPRYIVDPELGLMV
                     GMRGHGFMLSQYLAKLYVDKLTGKPTPDYFKELVLSGKGLCESAFK"
     gene            complement(8372..10606)
                     /locus_tag="PKN53_09075"
     CDS             complement(8372..10606)
                     /locus_tag="PKN53_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOD89140.1"
                     /translation="MSNEKKFGTSAVYFTALSTIVGAIVFLRFGFAVGSVGLWGTFLL
                     IIIGHLVTIPTALAISELATNKRVEGGGEYFIISRSFGLNIGATLGILLYLTQTISVA
                     FYIVAFTEAFDFLFNYFQNNLGIVLPKQVISVPAMLLLSVLVLKKGANMGLKVLYIAG
                     SVVLIAILMFFFGKPVDGPGIDYFSANATMKNTGDMFFVLAIIFPAFTGITAGVGLSG
                     DLKNPGKSIPIGTTAATFTGLILYLLICLKFANSALPADLLNNQLIMSDIAIGGKILI
                     PLGLAACTFTSALSSIMVGPRTLQALALDDSLPIKSRNKWLSKTRKKDNEPINASFVT
                     CIIAMIFVAFGSVDSVAQIISMFFLVTYGSLCLISFLHHFGSSPAYRPTFKSKWYISF
                     AGFIISVWAMFQINFVYTLVAYGAIVLLYIYMEHYHSDRKGFSAIFANTIFQMNRGLQ
                     IYLQNKRKKASEQNNTEEEWRPSAICISRNTFKRDSALKLLNWISYKYGFGTYLHLID
                     GYYSKKTNEQAQAELKRLLSNLDSSNQVYIDTLISPSITSAISQSIQTPGISGMDNNM
                     VIFEYLKSKPENDLHDIITNFRMVNAGGFDVCILGTSRKPLLYKNGIHIWASRFDTNN
                     TNLMILLGFIIMGHPSWKRANIKIFNVCYAQDAEEIRQNMHELINSGRMPITDTNIEI
                     IVRDGNTSIKEIINKRSIDAGLTMVGFDENSFKKDDDISLFEGYDQIGNVLFVHSNGE
                     KVIK"
BASE COUNT         3913 a         1499 c         1911 g         3409 t
ORIGIN      
        1 taattaacaa tcaatttgtt gtctttttat taacaatttc tgaatatccc taattagctt
       61 attagcattt tttgctttgt cgttaaatac agcttttgca caaaacgata gcatttatgt
      121 aatgaaagat ggtaatatat tatggcaata taaagttact gaaattgaca gtattatttt
      181 ttataaacct gtaattgaag aagaaacaag cggaactttt gttgatgaac gcgataatag
      241 agaatactca tgggtaaaaa ttggcgaaca agtttggatg gcagagaact taaaatattt
      301 accaagtgta gtaggccctg gtacaggttc taatactgag gcttattatt atgtttacga
      361 ctatgatggc actaatgttg acgcagctaa agcaacagaa aattatgcta cttatggtgt
      421 cttgtataat tggaccgcag ctatggctgg agcagtaagt agtgatgcta acccaagtgg
      481 agtacaaggc atttgtcctg atggttggca cttaccaagt gatgcagaat ggacacaatt
      541 aaaagattat ctgggtgaaa atgcaggagg taaacttaaa gaagttggaa caacacattg
      601 ggcagaccca aatacaggtg caactaatga atctggcttt acagctctgc caggtggtgg
      661 acgtagccac gaaggtaatt tctacgatgt tggaaacctc ggtgtttggt ggttttctac
      721 cgagtacgaa tctgataaag cgtgggggtg gtatctaggg aacagtaata gtggaatttt
      781 tagtggctac ggttacagga atgaagacta ttctatccgt tgtgtgaggg acaattagtt
      841 gaaagtttat aaagtttaaa gttcataaag aaaaaaagct aaatccgagc gagcagagtg
      901 agcttgcgaa ctctgcgaag caatagacgc gccagctact ttcaaacttt cgacttttaa
      961 cttttaactg atttcctgaa atgttttgat gcctgcacca aagcttcatc ttttaaaata
     1021 gctctcatgg ctaaaaagca taaaataaca gaaattaatg gtaaaataat taaaattcct
     1081 gtgctatatt ctaattctaa acgctttgaa atatctatta aataataaaa taaaagacca
     1141 aaacagccaa tttgtaacaa aatattaaaa actgtaagtc taacttgaag catgcgtttt
     1201 ttgtaaagaa aaattgtgaa aaaacttata aaacaaacta aacttaccag tattaataga
     1261 ggtaacattg aatattccaa aacaatgtct tggttcaaag cattgttgtg ataagaaaaa
     1321 ttagactctg taatttttat tggaatttct tgtaattctg gtgtaatgag ataaccaaaa
     1381 ggcgaaaaaa agaatattcc aaaacaaata aaactaacaa ataaatataa agtttgaatt
     1441 ctttgtatca taattttttt tgcaacaaaa ttacaataaa aatttaaaat actaacaagt
     1501 gtttttttgt cttatataaa taattaactt tgtgctttta aaattttaat attgaactta
     1561 tgaattataa agataatgtt gaggctatca atgcgttacg tcagaaatat gaaagtttga
     1621 tttctgaaat aaccaagaaa atttatgggc aagatcaagt tgtgaaagaa gttgttattt
     1681 ctatttttag tcgtggacat tgccttttag taggagtgcc aggtttagct aagactttgt
     1741 tagttaatac tatagcagaa gttttaggct taaaaaataa gagaatacag tttacacctg
     1801 acttaatgcc ttcagatata attggaacag aaatattaac tgaagataga caattcaaat
     1861 ttgtaaaagg accaattttc gctaatttta ttttagcaga cgaaataaac agaactccac
     1921 caaaaactca ggcagcactt ttggaggcaa tgcaagagcg ttcggttaca gcagcaggac
     1981 aagaatataa attagacgag ccattttttg ttatggctac tcaaaatcca atcgagcaag
     2041 aaggtactta tcccttgcca gaagctcaac ttgataggtt tatgtttaat ataattttgg
     2101 attatcctgc ctatgaagat gaaattacag ttgtgaaaaa tacaactgtt gataaagaat
     2161 ttgaattaaa acaagtgctt actgctgaag aaattatatt tttccaagga ttgatacgaa
     2221 aaattccaat taatgataat gttttggaat atgcagttaa attagcttgc aaaactcgtc
     2281 caaattctga atttgcacct gaaattacaa ataaatattt gcattgggga gcaggtccgc
     2341 gtgcttcgca atatttagta ataggagcaa aaactcatgc ggttttaaat ggaaaatata
     2401 cacctgatat tgaggacgtt aaagcagttg cgaaaataat tttgcgtcat aggataatta
     2461 aaaattataa agccgaagct gatggcataa gtattgatga tattattgat gaactattaa
     2521 aataactaat gctcagaaat attagcaaaa tagcttgtct cgttttttgc tttttgtttt
     2581 ttgcaatttt gactaatgct caagataaga aagacgaaaa acctagagtt gtagaaattt
     2641 taagagcaag ttcgttggaa tatgacgaat caacaggggt tactgcaaaa agactgttag
     2701 gagacgtggt gtttagacac gaagacgcat atatgttttg tgactcagca tggttttttg
     2761 atgatgtaaa tacaattaaa gcttataata atgttagaat aaaacaaggc gattctattt
     2821 tgatggtcgg aaaatatctt gaatatgacg gcaatactaa aatcgctaaa atgagagatt
     2881 ctgttatttt aaaacacaat aaatcttttt taattaccga ctcacttgat tatgatagaa
     2941 atattgatgt agcatattat ttcgaaggcg gaaaaattta tgatgaagat aatcgcttgt
     3001 ttagcagacg aggatattat tataccaaag ataaagatta ttatgcagtt gatacagtag
     3061 ttctcaaaaa tcctcagtac gatatttttt cagatacatt gaaatataat acagagtcaa
     3121 ctattgctta tttctttggt ccaacaaata ttgtgtcaga ttcaaaccac ttgtattgcg
     3181 aactcggata ttatgataca agcgaggata aggcttcgct atcaaataat gcatggttaa
     3241 ggtcaggaca gaattatctt ttgggagata gtttgtttta tgatagaaaa attagatatg
     3301 gcgaagcttt tatgaatgta agcgtagtgg ataccatcga aaatttggtg gcaaaaggaa
     3361 actatggata ttattacgaa gagccacaaa atgcaatgct tacaaaaaat acagaagtta
     3421 tttatgttag cgatggagat agtattttta tgcattctga tacagtttat atatctgttg
     3481 atactgccga ttttaaattg atacgagctt tctataaagt tcaggttttt aaatccgaca
     3541 tgcaagcaag atgtgattct ttagtatttt attcttacga ttctattgct catatgtttc
     3601 ataatccagt tttgtgggct gaaaatagtc aaattacagc caatcatatc gaaatgcact
     3661 tcttagataa aaaaccagat aaattttatt taaataataa tgcatttgcg atagaacaat
     3721 gtgatagcgt gcattttaat caacttaaaa gtagaaaaat gtctggctat atagttgaga
     3781 aaaaagtacg acaaatagac ctacataatg attgtcaaac tatttacttt attgttgatg
     3841 aagaagttga tgaaattgtt gccttaaata aagttgttag tacaaatatg agaatttggt
     3901 taaaagataa taaagttgaa aacgtttggt tcttcgacaa acccgatggt gaaaccattc
     3961 ctatagaaca acttaaagac gaacaaacct ttcttaaaga ttttagattt ttagacgaat
     4021 atagaccaaa atctagagaa gatattttta tctggaaaga aattccacaa aatgagtaaa
     4081 taaatccttg atattgattg ttttaataat atttttatta tttttataaa aaatcaatat
     4141 tatggaaaag aataaattac taattgctgt tcttggcaac gaaggatctg gaaaaacaac
     4201 tacttgggaa actttgttcg gacgtaaagt gcatacagga aaaaacctaa gaaacttagt
     4261 aattcaagga attaaaatcc cagttttttt aattaatgaa tcgcctctgg aaagaaaaac
     4321 aaaattagag tatatattac cagacgatga cccagaaatt gttataagtt cttttttata
     4381 ccataaagac gtagtcgaaa attttgaata ttttattaat agaggttata atatttatgt
     4441 gcaatggtta aatccaggct ataatgacaa aaaccataaa gcactgtttt ataacgatgg
     4501 tattataaat tatcttttaa gatgcggagc tatagtttct gcaaaaaatg cgaaaaataa
     4561 tccagtttca agagttaatg aaattaaaaa ttatttatta agctattttt taacaaaaaa
     4621 caacttaaac gtgagcatta agtcgcaatg agtagccgat tagtatttga acaaatgttg
     4681 tacgtataag aattagaaaa gatttgtggt gttgttgttt ttttcttaat tttgaagtga
     4741 gaaaaaagtg aaattataaa tctataataa ttatgggaat tgtaggagca cataacatag
     4801 gaaaagtttt agaacttaaa ttaaacgagg ttggtattac aactctaaaa gaattaaaag
     4861 aaatgggaac tgaaaaaaca tttcttcttc tttacgaaaa aaataaaaat acaactagag
     4921 caactttatt gtctatagaa ggagctattc aagaaataag atggcataat ttagacgaaa
     4981 aacgcgttgc ggaattgcgt gaattttatg atagtgtagt ggaaaaaaga aaataatgaa
     5041 aaatgtgaca aaactcattg cttgagattg ttaattaatt caattttact ttttaaaact
     5101 atatctacat aataagattt tttatgcaaa aaattgaaaa acatccgatt ttaaaagttc
     5161 ctgaaaaaaa cgaacattta ttttatttta acgataaagc cgtagtagga actaaaggtt
     5221 ttactatagc cgcagccttg catcaagcag gttttcctgt acatagccat tctttagata
     5281 atagaaaaag atctttggaa tgtggtatag gaaaatgtgg agcttgcgaa atgcttgttg
     5341 atggtaaaat aaaacgtatt tgtattacta aagttgacgg aattagagaa gttagagaaa
     5401 ttccaaaaga ttttgaacct agtcaggaaa aaatcaattt aaaagacgaa atttctattt
     5461 ttaaaactca agcagttatt ataggtgctg gtccagcagg attagctgct cgagaggttt
     5521 taaatcaaca cgaagtttca aatattgttg ttgacaataa cgataaaatc ggtggacagt
     5581 ttttaatgca aactcaccaa tttttctttt ttgaaaaaga gaaaaaattt ggtgggcttc
     5641 gtggtttcga aattgcagaa actttagcag gagacaatag agaaggaata cttttaaact
     5701 ccgtagtttg ggatatatta gaaggtccaa gacttgctgt gaaaaatata acaactaacg
     5761 aaatatttta tatagatgct gaatatatca taattgcaac aggagcaatc ccatttatgc
     5821 catcgtttaa aaatgatgat gtgccaggag tttatactgc tgcagtagtc caaaaaatga
     5881 tgaacaacga gttcactctt ttaggaaaaa atattttaac tgtaggtgca ggaaatattg
     5941 gttatctaac ttcttaccaa ctaatgcaag caggagcaaa agtaaaagca attatcgaag
     6001 ctcaacctaa cgaaggaggt tttcccgttc aagcaaatag acttagaaga ttgggaattc
     6061 caattatgac aagccatatt ttaattgaag caattccaaa tgaagactat acaggtataa
     6121 caggtgcagt aattgcagaa tgcgaaaatt ttaaacttat accaggaaca gaaaaaatta
     6181 ttacaggaat tgatgcaata aatatttgca caggcttagt tcctgataat caattagtaa
     6241 caaaagcaaa tcttttattt ggacgtaaat cttttgcaat aggcgatgct ttaagaattg
     6301 gagaaggcac atcagcagtt ttacgcggaa aacaagcagc ttacgaactt atcgatgctt
     6361 taggaaaaag atataattat actgattatc ttagtgtttc aaaagaatat attgaatctc
     6421 aacaacatcc aacacgtata attcaagaac ctaacaaacc tacacgcgag agagccgaag
     6481 caaaacctta tgtgataatg gattgtttat atggctttgc ttgtaatcct tgtacttttt
     6541 cttgtaaaca aggtgcaata accaaacttt caacaagcac ggtaccggtt atagactatg
     6601 acaaatgtat aggttgtatg gattgtgttt accaatgtcc tggacttgca atttttggtt
     6661 attcagagaa aaataattgg ctatttttac caatagaata cgaagttgaa gaaaattcag
     6721 aagtttattt ggtaaataat aatggcgaaa aaataggcga ggggataatt gaaaaaattc
     6781 tttataaacc taataaaaca aatgttgcta gggtaaaagc acttgatttg caaggcgaac
     6841 aaatgcaagc agctcgtggt tttattgtaa agaaagatta tccacaacct ttagaattta
     6901 aaaatgtaaa aggcgaaagc caagctgaaa cttacatttg tcattgcgat gatgtaaaat
     6961 tagaagatat tttaaaaatc attggtgata gaaaatttat ttctattgat gaaattaaac
     7021 atattactcg tttgggaatg ggcgcttgtc gtggaaagag atgcatcaaa cgtttaaaac
     7081 aaacaatttc tgcatacgga attgaaattg ttggcgatgc aacacctcgt gctccgttat
     7141 caaatcaagt gagcatgggc gagctatatc cacatgaatc tgaaagaatt tttacaaatg
     7201 taaataagaa atcaaacaaa agaattaaag ttcaagctct tgttgcaggt ggtggaatgg
     7261 caggaagttc tctgtttaag tttatggcag aagcaggaat gaaacctgtt ttggtaaatc
     7321 gtgagcgtgg aagttcttgg cgtaatattg ccggcggaag accagccttc tctttaccag
     7381 cattggcaga catagcacta aaaaatagag agctttttat tgaattgcaa aatatttgcg
     7441 atattgatta tgttccaact aaatatgtga gttttgctca tgataatgaa acttataaag
     7501 ctcttgaggc ttctaaaagt tggtctgatg cttttatggt agaaaaagtt gattttagaa
     7561 aagaaatttc accatatttc aataataatt tagatactta ttctgctgca ttaattacta
     7621 atgactgttg gcaagcaact ccaggtaaag ttttagactt attacgcaaa attggaattg
     7681 aaaaaggcgg gcaagtttta gaagattgcg aacttattga tttacaaaga gaaggaaaaa
     7741 atgttatagc acttgtaaaa actcatacag gcgaatttgt tgaatatgaa accgaacatt
     7801 ttgtaaatgc tcttggcaat gaatctagta aatttgctaa aaaacttggt cttgaaatag
     7861 gtttgtatcc tgttaagcat caggcattta taacaaggcg tttgccttta ctaggtaaag
     7921 atggcaagac tttagatatg ttaattgaca gaagacaata taaaggattt tctgctgttt
     7981 atggtcaaca acttgcaact actggtcaaa ttataggttg tgcttcgcct ttaatcgatg
     8041 ctttagaaac agataaagaa gttaaaacta acgcaaaaga cttcttagaa atagtttctg
     8101 aagtatttgt agattggatt ccacaattat caagtgttgg atttcaagct gcttgggcag
     8161 gctattatat tgaacctcga tatattgttg acccagaatt aggtttaatg gttggaatgc
     8221 gtggacatgg ctttatgtta agtcaatatt tagcaaaact ttatgttgat aaattaacag
     8281 gaaaaccaac tcctgactac tttaaagaat tagttttatc aggaaaaggc ttgtgcgaat
     8341 ctgcttttaa ataagaaaat agcttaaagt tttatttaat tactttttcg ccatttgaat
     8401 gtacaaatag aacatttcca atttgatcat acccttcaaa aagcgagatg tcgtcatctt
     8461 ttttaaatga attttcatca aaaccaacca tagttagtcc tgcgtcaata gaacgtttgt
     8521 tgataatttc ttttatagat gtatttccgt cacgtacaat aatttcaata ttggtatctg
     8581 taataggcat acgtccgctg ttaatcagtt cgtgcatatt ttgtctgatt tcttcggcat
     8641 cttgagcgta acatacgtta aagattttaa tatttgctct tttccaactt gggtgcccca
     8701 ttataataaa cccaagtaaa atcattaaat tagtattatt agtatcaaaa cgacttgccc
     8761 aaatgtggat accattttta tataaaagtg gtttgcgact tgttcctaaa atgcaaacat
     8821 caaatccacc tgcgtttacc atgcggaaat tagttattat atcatgcaag tcgttctctg
     8881 gtttgctttt cagatattca aaaataacca tattattatc catacctgaa attcctggtg
     8941 tttgaataga ttgcgaaata gccgatgtga ttgatggcga aatgagagtg tcgatataaa
     9001 cttgattaga tgaatcaaga ttgctaagta aacgttttag ttcagcttgt gcttgttcgt
     9061 tggttttttt tgagtagtaa ccatcaatta agtgcaaata agtaccaaat ccatatttat
     9121 aagaaatcca gttaagtagc tttagggcag agtctcgctt aaaagtattt ctagaaatac
     9181 agatcgcact aggacgccac tcttcttccg tattattttg ctccgaagct tttttccttt
     9241 tattttgaag atatatttgt aatcctctat tcatttggaa aatagtgttt gcaaatattg
     9301 ccgaaaatcc tttgcggtca ctgtgataat gttccatata aatatatagt aaaactatag
     9361 ctccgtatgc tactagtgta taaacaaagt taatttgaaa cattgcccat actgaaatta
     9421 taaatcctgc aaaagaaata taccatttcg atttgaatgt cggtctatat gctggagaag
     9481 aaccaaaatg gtgtagaaaa gaaattaaac ataaacttcc gtatgtaact aagaaaaaca
     9541 tagaaatgat ttgtgctacc gagtcaacac taccaaaagc aacaaaaatc atagctataa
     9601 tgcatgtaac aaatgaagca ttaataggtt cattatcttt ttttctagtt ttgctcaacc
     9661 atttatttct gctttttatt ggtagagaat cgtctaaagc aagagcttgt agtgtgcgtg
     9721 gtcccaccat aatagagctt aatgcagatg tgaaagtaca agcagctaag ccaagcggaa
     9781 ttagaatttt tccaccaata gcaatgtcag acataattaa ttggttgttt aataaatcag
     9841 ctggtagtgc agagtttgca aattttaagc aaataagtaa atataaaatc aagccagtaa
     9901 aggttgcggc tgttgtgcca ataggaattg attttccggg attttttaaa tcgccagaaa
     9961 gtccaacgcc agcagtaatt ccagtaaagg caggaaatat aattgctaaa acaaaaaaca
    10021 tatctccagt atttttcatt gtagcatttg cactaaaata atctatacct ggtccgtcaa
    10081 ctggtttgcc aaagaaaaac attaatatag caatcaaaac aacagagcct gcaatataca
    10141 atacttttaa acccatatta gcaccttttt tgagaactaa tacagaaagt aggagcatag
    10201 caggaacact aattacttgc tttggaagaa caattcctaa attattctga aaataattaa
    10261 agagaaaatc aaaagcttct gtaaatgcca caatataaaa ggctacgcta attgtttgag
    10321 taagatatag tagtatgccc aaagtagcac caatatttag accaaatgaa cgtgaaataa
    10381 taaaatattc gcctccacct tcaacacgtt tgtttgtagc taattcagat atagcaagtg
    10441 cagtaggaat tgtaactaaa tgtccaatta taataagtaa aaatgttccc cacaaaccaa
    10501 cagaacctac tgcaaaccca aaacgcaaaa aaacaattgc accaacaata gtagaaagag
    10561 cggtaaaata aacggctgat gttccaaatt ttttttcgtt actcataaca agatagcttt
    10621 atgttctctt ggtacaaaga tatttatttt tgagtaaaac tctattttta taagaaataa
    10681 aatatgtttt tttattaaca tttgatttgt aagattttat tttctgtata aa
//
LOCUS       DAOPNX010000088        10335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_7362_length_10335_cov_5.717802, whole genome shotgun sequence.
ACCESSION   DAOPNX010000088 DAOPNX010000000
VERSION     DAOPNX010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10335
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7362_length_10335_cov_5.717802"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /gene="ispF"
                     /locus_tag="PKN53_09080"
     CDS             complement(<1..464)
                     /gene="ispF"
                     /locus_tag="PKN53_09080"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOD89141.1"
                     /translation="MNFKIGIGYDVHALQEGLDFWLGGVKIEHNKGCLAYSDGDVLIH
                     AICDAILGAANLRDIGYNFPDTDNKYKGIDICILLQKSLELINNKGYKIVNVDSVILL
                     QTPKLKDYIPLMKQKLASVMEIDIEDISIKASTTEKLGFVGREEGIVAQAVV"
     gene            complement(467..1726)
                     /gene="porV"
                     /locus_tag="PKN53_09085"
     CDS             complement(467..1726)
                     /gene="porV"
                     /locus_tag="PKN53_09085"
                     /inference="COORDINATES: protein motif:HMM:NF033710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HOD89142.1"
                     /translation="MMKRTFLILLITIVLGNSINAQVSTNDDIVGRDAPNTITTTVPF
                     LLIAPDSRSGALGDAGVATTPDVNSLHWNPAKYNFIDNNVGVSISYTPWLRQLVRDMN
                     LAYVSGYKKFNKENVLSASLLYFDLGSIQFRSVDNVLLRDFSPHEFAIDAAYSRLLGE
                     KFSSSVAMRFIYSNLTGGVEVQGNDSYPGKTVAADVAFYYYNDEIELSGMKSTLMWGL
                     NISNIGAKISYTTNEYRDFIPTNFRTGVGYKLALDDHNEFMFTFDLNKLLVPTPPQYY
                     QAGEILPNGDTVIPSDKIIKYGKDPKISVGSALFRSWYDAPGTATAFVKNGKPSVFRE
                     EMAEFTISAGVEYWYDKLFALRFGYFNEHQYKGNRKYFTVGAGLKMNVFGLDFAYLIP
                     TTQANPLQHTLRFTLIFDLGGLAAEKS"
     gene            complement(1823..5641)
                     /gene="porU"
                     /locus_tag="PKN53_09090"
     CDS             complement(1823..5641)
                     /gene="porU"
                     /locus_tag="PKN53_09090"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOD89143.1"
                     /translation="MRKITLFFVSFFVSNLLISQNILISDSIIWQDNNIFNNNDKNTS
                     NILYFVNCNQRSSDDLPIYYKRLPINNNVEIISIDEISVDYQVVNKSDYENVNKLENL
                     TNEFQTKAWHSKYRKKDFLAFEVLPLRINPNTNELEKLIRFEYKINYRFVSEKKSQQT
                     YATNSKMANGTWVKIKVEKPGVYKLTYEQLQEMGFSNFSNIGVFGYGGMLPKTVGQVI
                     ADDMPERPLLKVDANSNSVFDGGDYILFYADGPHSIKFTSTLFSHEYHNYSLSSYYFV
                     SDRGSFKAPINISSSNSFDKTVTTYDDYLFLEKDSINLIKSGRTWFWREFDYYLNHEF
                     NLNVKNISLSDTAIVRVRVAARSSVSSNFKLSINGINQPNIPIASVSNSTTEVFARQN
                     DSAKINTFRVLPSSENFKFNLTYTKTATNSKGWLDYISVQLRRKLSLTDTGFLVFRDT
                     KSVENNTKVKFIVSGANSSSLIWDITDRFNAKQISSNISGDKLSFVAESNELREYLVF
                     DYKANFPSPIYSGSADVGHVDNQNLHSHQPVDLVIVSPSEFMSQAKEIKDIHEQYDNM
                     SVVIVDPEKIYNEFSSGTPDVSALRNYMKMLYERADVNNIPENLLLLGNGSYDNMSKD
                     PQVANHILTYESVNSLGPTASFVSDDYYVFLDDGEGGFLGTHDMDMGVGRLPIKSASE
                     ASDYVKKLREYYQPIAYGNWKNNILLVADDADKGETLHQNQTNTLATQIESTYPIFNL
                     EKIFLDDFEQVSTVEGHRYPDVNQAITDFVHNGVLSVSWIGHGNPNTWADERVLTTNM
                     VKSWRNGPKYPIFVTATCEFAPYDNHHIVSAGEEIILNPNGGGIALFGTTRLAFAGSN
                     AHLAYKFYNNIFNIDGSGKVSTLGISVANTKNQQGSDTNKRVFAFLGDPAVRPSVPEY
                     KVFTTKINGIDANVFNDTISAMEKVVFEGVIKKADNSIFENFNGSIYPVVYDKRQNYT
                     TRGNDGSQPLDYTAQKNIIFKGNASVVNGKFKFEFIVPVDIAYFYDEGKVSYYAHNNL
                     NLEASGYDDSFIIGGSSDNAITDNEGPEIDLFMNDENFISGGITDENPILLAKFFDES
                     GINTVGSGIGHDITMIIDEKTSEAIVLNKFYESNKDDYKSGELRYPLNNLELGPHSLK
                     LKAWDVLNNSSEAVTDFIVANSSELFIDNLFNYPNPFSTYTEFYFDHNQPYVDLKVLI
                     QIFTVAGNHVKTIETDMFSTGYRSQPIPWDGKDEYGDKIGKGVYIYKVKVKSPNGNII
                     DKFEKLVILN"
     gene            5782..6768
                     /locus_tag="PKN53_09095"
     CDS             5782..6768
                     /locus_tag="PKN53_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOD89144.1"
                     /translation="MVKHFIFLLSALLLSSLTVLNAQDAHFTQFYANSLYLNPAFAGA
                     ERCPKISLNYRNQWPAMGPTYVTYSASYDQHLNALQGGVGLLLMNDVQGGGVINTTTI
                     SGMYAYTLPVSRRFFIAGGFEASFVMKSINWDFIFPNMIHPLYGPIYASLENPDIIND
                     KRNYVDFSAGIIAFSEKTFFGVAVHHLTQPSEAFLRGSDAVLPRKLTAHFGTEVVINS
                     SSFRRGDLKIAPQLLYHQQGQFQLFNWGLYVSRRGIIAGFWARQNFNFNYDSFIMLAG
                     YKIDKIRFAYSYDLTVSNLKNSTYGAHEVSVAYTFDCHVKDKKLSTISCPMF"
     gene            6803..8782
                     /locus_tag="PKN53_09100"
     CDS             6803..8782
                     /locus_tag="PKN53_09100"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOD89145.1"
                     /translation="MKKNVLFILIVLVASILASSCKKEVSNTTGWNYRDPNFGGFENH
                     PGYEQETGPGLVFIEGGSFAMGRVEQDVMYDWNNIPRRVTVSSFYMDETEIRNIDYNE
                     YLYWLSYVYSSDYRFLYREALPDTLVWRERLAFNEPMVELYLRHPAYEEYPVVGVSWV
                     QASKYCDWRTDRVNELILRREGIIDKYLAPQDQAKHFNLDAYLIYAENLDLPIDETGK
                     GPDGKIQNLYVDPAVLKAGRKGGRVNTGDDRRWVRMEDGYFLPRYRLPTEAEWEFAAL
                     AHIGNHYKERIYERRLYPWNGHYIRNDSRGVERGLMMANSMRGRGDNMGVAGALNDKA
                     DITAPVRSYWPNEYGLYCMAGNVNEWVMDVYRDLTFEDMAEFRPFRGNIYTDYQVIID
                     PRSGDIELTPEAENYFDYSGKIRRKPVRDEACFSRENYTTADNRNYLDGDLESLIPQM
                     GTSEDYWTKDERFQETVSNQTKVYDPVSEELTAENVGLIYPGATLMSGGNEVLLPNGI
                     KVDLVNKTYILPTGHEKTAEGVIVMPDGSTVLPGGELQLADGSIIYPDQFFRKRTKDM
                     YQEGEWGEHGEWGYGQYDPRNPKINKGNSAQPNKAMSSLVSDRSRVFKGGSWKDRAYW
                     MVPGTRRFLDEKMGRADLGFRCAMDRVGGPIQSKK"
     gene            8833..10125
                     /gene="murF"
                     /locus_tag="PKN53_09105"
     CDS             8833..10125
                     /gene="murF"
                     /locus_tag="PKN53_09105"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOD89146.1"
                     /translation="MISIEKIYSLFKKSSGINTDSRVKQKNALFFALSGENFDGNEFV
                     ENAIENGCLAAICSNKRLANNENIFYVENTLNTLQKLANYHRKQLKTPIIAITGTNGK
                     TTTKELISTILAQTHRVAYTSGNKNNHIGVPLTILSMNRTVDIGIVEMGANHQGEIKT
                     LCEIAEPNYGIITNVGKAHLEGFGSFEIIKQTKAELYDYLKLKNGEIFINADNPLLVE
                     MLGNYSAIKYGIDSNYFCSGKYSEISLQATVSWKSKNEKGIAKSNLIGAYNFENILAA
                     ITIGQYFKVPGTAIDNAILRYYPNNNRSQLVKTNRNNLILDYYNANPTSMELAIKNFE
                     NIKDNSKGLILGDMLELGEDSEIEHKKILEYISNKSFKKVYLIGPHFYKFKEEYGFNF
                     FNSVDDFAKHIVKNPETGNFFLIKGSRGVKLEKCTDYL"
BASE COUNT         3634 a         1670 c         1567 g         3464 t
ORIGIN      
        1 aaaactacag cctgagcaac tattccctcc tctctcccaa caaatccgag tttttctgtt
       61 gtgctggctt ttatagaaat atcttcaata tcaatttcca taactgaagc caatttttgt
      121 ttcattaaag ggatataatc ttttagcttt ggtgtttgca ataaaattac agaatcgaca
      181 tttacaattt tatagccttt attattaata agttctaaag atttttgcaa caaaatgcaa
      241 atatcaattc ctttatattt attatcagta tcaggaaagt tatagccaat atctcttaaa
      301 tttgctgcac ctaatatagc atcacaaatt gcatgaatta atacatctcc gtcagaataa
      361 gcgaggcaac ctttattgtg ttcaattttt actccgccta gccaaaaatc taaaccttct
      421 tgtaaagcat gaacatcgta tccaatgcca attttaaaat tcatttttaa cttttttctg
      481 ccgctaagcc tccgaggtca aaaattaatg taaatctaag cgtatgttgc aatgggtttg
      541 cttgagttgt tggaattaga taagcaaaat ctaaaccaaa aacattcatt tttaaaccag
      601 ctccaacggt aaaatatttt ctatttcctt tatattgatg ctcgttaaaa tatccaaaac
      661 gcaaagcaaa caatttgtca taccaatatt ctacgccagc cgagattgta aattcagcca
      721 tttcttctct aaatacagaa ggttttccat ttttaacaaa agctgtagca gttcctggtg
      781 catcatacca agatctaaat aaagcagaac ctacagatat tttaggatcc ttaccgtatt
      841 tgataatttt atcgctaggt ataactgtgt caccgtttgg taatatttct ccggcttgat
      901 aatattgagg tggtgttgga actaataatt tgttcaaatc aaaagtaaac ataaactcat
      961 tatgatcgtc aagagcaagt ttataaccaa ctcctgtgcg aaaatttgtt ggaataaaat
     1021 ccctatactc gttggttgtg tatgagattt tagcaccaat atttgaaatg tttaatcccc
     1081 acatcaaagt tgatttcatt ccgcttagtt cgatttcgtc attataataa taaaaagcaa
     1141 catctgcggc aacagttttt ccaggataag aatcatttcc ttgcacttca acacctccag
     1201 ttaaatttga gtatataaaa cgcatggcaa cagagcttga aaatttttct cctaacaaac
     1261 ggctataagc agcatctata gcaaattcat gtggagaaaa atcacgcaac aatacattat
     1321 ctacacttct aaactgaatg ctacctaaat caaagtacaa tagagatgca ctcaaaacat
     1381 tttctttatt aaacttttta tacccactaa cgtaagccaa gttcatgtct ctaaccaatt
     1441 gtctcaacca aggagtataa gaaatagata caccaacatt attatcaata aaattatatt
     1501 ttgcaggatt ccaatgcaaa gagtttacgt caggcgtagt agccacacct gcatctccta
     1561 aagctcctga tcttgaatct ggtgcaatta ataaaaatgg aaccgttgtt gttattgtgt
     1621 taggggcatc acgtccaact atatcatcat ttgtacttac ttgagcattt atactattac
     1681 ctaaaactat tgtaattaat aaaataagaa atgttctctt catcatattt ttatgtttga
     1741 aattttgaca aatatattac atttttttta aacttcatta gtaacgtaaa atattatttt
     1801 ttattgtctt ttaaattgat ttttaattca aaattactaa tttttcaaat ttatcaataa
     1861 tattgccatt aggagatttt accttcacct tatatatata tactcctttt cctattttat
     1921 ctccatattc atcttttccg tcccaaggta ttggctgact tctatatcct gtgctaaaca
     1981 tgtcggtttc aatagtttta acatgatttc ctgctactgt gaatatttga attaaaactt
     2041 ttaaatcaac ataaggctgg ttatggtcaa agtaaaattc ggtatatgtt gaaaatggat
     2101 ttggatagtt aaaaagatta tcaataaaaa gttctgaaga attagcaaca ataaaatctg
     2161 taactgcctc actactatta tttaaaacat cccaagcctt tagtttaaga ctatgtggac
     2221 caagttctaa attatttaga ggataacgta attctcctga tttataatca tctttattag
     2281 attcataaaa tttatttaac actatagctt ctgaagtttt ttcatctata atcatagtta
     2341 tgtcatgacc tattccacta cccacagtat taatacctga ttcatcaaaa aacttagcca
     2401 aaagaatagg attttcatcg gtaataccgc cagaaataaa gttttcatca ttcataaaca
     2461 agtcaatttc aggaccttca ttatctgtaa ttgcattatc tgatgatcca cctataataa
     2521 aactatcgtc atatccactt gcttcgagat tcaaattatt gtgtgcataa taactaactt
     2581 ttccttcatc ataaaaataa gcaatatcta cgggtacaat aaattcaaat ttgaattttc
     2641 catttactac tgaagcattt cctttaaaaa taatattttt ttgagcagta taatccaatg
     2701 gttggcttcc atcatttcca cgtgttgtat agttttgtct tttatcatat acaactgggt
     2761 aaattgagcc attaaagttt tcaaatatac tattgtcggc ttttttaatt acaccttcga
     2821 atacaacttt ttccattgca gaaattgtat cgttaaaaac gttagcatct ataccattta
     2881 tctttgtagt aaacacctta tactcaggaa ctgatggacg cacagctgga tctcctaaaa
     2941 aagcaaaaac ccttttatta gtatctgaac cttgctgatt ttttgtattt gctaccgata
     3001 ttcctagagt agaaaccttt ccgctaccat ctatattaaa aatattatta taaaacttat
     3061 aagctaagtg ggcattactt cctgcaaaag ccaaccgagt agttccaaac aaggcaattc
     3121 cgccaccatt tggattcaat attatttctt ctccagcaga tacaatatga tgattgtcgt
     3181 aaggagcaaa ttcacaagta gctgtaacaa aaataggata tttaggtcca tttctccaac
     3241 ttttaaccat atttgtggtc aatactcttt catctgccca tgtgttagga tttccatgtc
     3301 ctatccaact tactgaaagc accccgttat gaacaaaatc agtaatcgcc tgattaacat
     3361 caggatacct atgcccttca acagtagaga cttgttcaaa atcgtctaaa aagatttttt
     3421 ctaaattgaa aattggataa gtactttcta tttgagttgc tagcgtatta gtttggtttt
     3481 ggtgcaacgt ttctccttta tctgcatcat ctgctactag taaaatatta tttttccaat
     3541 taccataagc tattggctga taatattctc ttaatttttt tacataatca gaagcttcac
     3601 ttgcactttt tattggcaat cttcctactc ccatatccat atcatgagtt cctaaaaatc
     3661 caccttcccc atcatctaag aacacataat aatcatctga aacaaaagaa gcagttggcc
     3721 ctaaagagtt taccgactca taggtcaata tatgatttgc aacttgcgga tctttagaca
     3781 tattgtcata acttccattg ccaagtaaaa gaagattttc gggaatatta tttacgtcag
     3841 ctctttcata aagcattttc atataatttc ttaaagctga aacatctggt gtccctgacg
     3901 aaaattcatt atatattttt tcagggtcaa caataacaac actcatatta tcgtattgct
     3961 catgaatatc tttaatctct tttgcttgag acataaattc gcttggacta acaatgacta
     4021 agtcaactgg ttgatgagaa tgcaagttct gattatcaac atgcccaaca tctgcagaac
     4081 cagaataaat tggcgaagga aaatttgctt tataatcaaa aactaaatat tctcgtaatt
     4141 cattactttc cgcaacgaaa cttaatttat cgccagaaat atttgatgaa atttgttttg
     4201 cattaaatct atctgtaata tcccaaatca aacttgaaga atttgcacca gaaacaataa
     4261 atttaacttt agtgttattt tcaacagatt ttgtatctct aaatactaaa aaaccagtat
     4321 cggttaaact tagttttcgt cttaattgta cagatatata atctaaccaa ccttttgaat
     4381 tagttgctgt ttttgtataa gttaaattaa atttaaaatt ctcagaacta ggcaaaaccc
     4441 taaaagtatt tatttttgca ctatcatttt ggcgagcaaa aacttcagtt gttgagtttg
     4501 aaacacttgc aataggtata tttggttgat ttataccatt tatgcttaat ttaaaattac
     4561 tactaaccga agacctagca gccactctta ctctcacaat agctgtatca gataaagaaa
     4621 tattttttac attcaaatta aactcgtgat ttaagtaata atcaaattct ctccaaaacc
     4681 atgttctacc agatttaatt aaattaatag aatctttttc taaaaataaa taatcatcgt
     4741 aagtggttac agttttatca aaactattag aagagctaat attaataggt gctttaaaac
     4801 tgcccctatc cgaaacaaaa taataagaag aaagagaata gttatgatat tcatgagaaa
     4861 aaagagtaga agtaaatttg attgagtgag gtccgtctgc ataaaataaa atataatctc
     4921 caccatcaaa aacagaatta gaattagcat caactttaag tagtggtctt tctggcatat
     4981 catcagctat tacttgcccc acggtcttag gcaacattcc accataacca aaaactccga
     5041 tatttgaaaa atttgaaaaa cccatttctt gcaattgttc ataagtcaat ttataaactc
     5101 ctggtttttc aactttaatt ttaacccaag tcccatttgc catttttgaa tttgttgcat
     5161 aagtttgctg tgattttttc tcactaacaa acctataatt gattttatat tcaaaacgaa
     5221 ttaatttttc tagttcattt gtatttggat taattcttaa aggcaaaact tcgaatgcta
     5281 aaaaatcttt ctttctatat ttagaatgcc aggcttttgt ttgaaattca tttgttaaat
     5341 tttctaattt attaacattt tcataatccg acttattaac aacctgataa tcaaccgaaa
     5401 tctcatctat cgaaattatt tccacattat tattaatagg taagcgttta taataaatcg
     5461 gcaagtcatc gctacttctt tgattacaat ttacaaaata taaaatattt gaagtatttt
     5521 tatcgttatt attaaaaata ttattatctt gccaaataat tgaatcggaa attaaaatat
     5581 tttgagatat taacagatta ctaacaaaaa aactaacaaa aaacagcgtg atttttctca
     5641 taaaaaaaat aaatttaaaa aaaaacaaaa aaaaagttct aaatattttt taaaacgaca
     5701 aatttagctt aatattgtaa attatttatt gtatttatga aaaaattaat ataaacgcgc
     5761 gttgtagata agatgaaaac tatggttaaa catttcatat ttttgttaag tgccttacta
     5821 ctaagtagtt tgacagtttt gaatgcacaa gatgcacatt ttacgcaatt ttatgcgaat
     5881 tcgctttatt tgaatcctgc atttgctggt gcagagcgat gtcctaaaat tagtttaaat
     5941 tataggaatc agtggcctgc tatgggtcct acctatgtta cttacagtgc ttcttatgac
     6001 caacacttaa atgcacttca aggtggagtt ggattgcttt taatgaacga tgttcaaggt
     6061 ggtggagtta ttaatactac tacaattagt ggtatgtatg cttacacttt acctgtatct
     6121 cgcagatttt ttattgctgg aggttttgaa gcatcttttg taatgaagtc tataaattgg
     6181 gattttattt ttccaaatat gattcaccca ttatatggac caatctatgc ctcgctagaa
     6241 aatccagaca taattaatga caaacgtaat tatgttgact tttcagcagg aattattgcc
     6301 ttcagtgaaa aaacattttt tggcgtagca gttcatcact taactcagcc ttcagaagcc
     6361 tttttacgtg gaagtgatgc tgtcttgcct agaaaactaa ccgctcactt tggaacagag
     6421 gttgtaataa atagtagcag tttccgaaga ggtgatttaa aaattgctcc tcaattatta
     6481 tatcaccaac aaggacagtt tcaattattc aactggggtt tatatgtttc gaggcgtggc
     6541 attatcgcag gattttgggc tagacaaaac ttcaacttta attatgattc tttcataatg
     6601 ttagctgggt ataaaataga caaaataaga tttgcttata gttatgattt aaccgtatca
     6661 aatttaaaaa actcaactta tggagctcac gaagtttctg ttgcatatac ttttgattgt
     6721 catgtaaaag ataagaagtt gtccacaata agttgtccta tgttttagtt attaattaag
     6781 aatcgtataa aatttttata atatgaaaaa aaatgtttta tttatcttaa tagtccttgt
     6841 cgcttcaatt ttggcaagtt cgtgcaaaaa agaagtatca aatactactg gttggaatta
     6901 tcgcgatcca aactttggtg gttttgaaaa tcaccctgga tatgagcaag aaactggacc
     6961 tggattagta tttattgaag gtggttcttt tgctatgggt agagttgaac aagatgttat
     7021 gtatgattgg aataatattc cacgtagagt aactgtttct tcattttata tggatgaaac
     7081 cgaaattaga aatattgatt ataatgaata tttatattgg ttgtcttatg tttattcttc
     7141 ggactacaga tttttatacc gagaagctct acccgacact cttgtttgga gagaacgctt
     7201 agcttttaac gaaccaatgg ttgaattata tttacgtcat cctgcatatg aagaatatcc
     7261 agttgttggt gttagttggg ttcaagcatc aaaatattgc gactggcgta cagatagagt
     7321 aaatgaacta atccttagaa gagaaggtat tattgataaa tatttagctc ctcaagatca
     7381 agcaaaacat tttaacttag atgcttatct tatttatgct gaaaacttag acctacctat
     7441 tgatgaaaca ggaaaaggtc ctgatggcaa aatacaaaat ttatacgttg accctgctgt
     7501 tttaaaagct ggcagaaaag gaggacgagt taatactggt gatgatagac gttgggtaag
     7561 aatggaagat ggatatttcc ttcctagata ccgcttacct accgaagctg aatgggaatt
     7621 tgctgcttta gctcatattg gaaaccatta caaagaaaga atttacgaaa gaagattata
     7681 cccatggaat ggtcattata ttagaaatga tagtcgtggc gttgaaagag gtttaatgat
     7741 ggcgaactca atgagaggac gtggggataa catgggggtt gctggtgctc taaacgataa
     7801 agcagacatt actgctcctg ttcgctctta ctggccaaat gaatacggat tatattgtat
     7861 ggcaggtaat gttaatgaat gggtaatgga cgtttataga gatcttactt ttgaagatat
     7921 ggcagaattt agaccattca gaggtaatat ttataccgac taccaagtaa taatagaccc
     7981 aagaagtgga gatattgaat taacccctga agcagaaaac tatttcgact acagtggaaa
     8041 aattcgtaga aaacctgtta gagacgaagc ttgctttagc cgtgaaaatt atactactgc
     8101 cgataataga aattatttag acggagattt agaatcttta atcccacaaa tgggaacttc
     8161 tgaagattat tggacaaaag atgaaagatt tcaagagact gtttctaacc aaactaaagt
     8221 atatgaccct gtaagtgaag agctaacagc tgaaaatgtt ggactgatat atcctggagc
     8281 aactcttatg tcgggcggaa acgaagtttt attaccaaac ggaataaaag ttgacttggt
     8341 aaataaaact tacatattac caacaggaca tgaaaaaact gccgaaggcg taattgtgat
     8401 gcctgatggt tctaccgttt tacccggtgg agaattgcaa ttagcagacg gaagcataat
     8461 ttatcctgac caattcttta gaaaacgtac taaagatatg tatcaagaag gtgaatgggg
     8521 agaacatgga gaatggggat atggacaata cgacccaaga aatccaaaaa tcaataaagg
     8581 taatagtgct caacccaaca aagcaatgtc atctttagta agtgacagat ctagagtatt
     8641 taaaggcggt tcatggaaag acagagcata ttggatggta ccaggtactc gtcgcttcct
     8701 tgatgagaaa atgggaagag cagatcttgg ctttagatgt gctatggaca gagttggtgg
     8761 tccaatacaa tctaaaaaat aaataaaaat ctcataactt aaaaccccaa tatttggggt
     8821 tttatttttt ttatgatatc aattgaaaaa atatacagtt tattcaaaaa aagtagcgga
     8881 ataaatactg atagtagagt aaaacaaaaa aatgctctat ttttcgcatt aagtggagaa
     8941 aattttgacg gcaacgaatt tgttgaaaat gcaatagaaa atggttgctt agcagcaatt
     9001 tgttcaaata aaagattagc taataatgaa aatatatttt atgttgaaaa tactttaaac
     9061 actcttcaaa aattagctaa ctatcaccgc aaacaattaa aaacaccaat tatagcaatt
     9121 acaggtacaa atggaaaaac aaccactaaa gaattaattt caacaatttt agctcaaaca
     9181 cacagagtag catatacaag tggaaataaa aacaatcata ttggtgtacc actaacaatt
     9241 ttgtcgatga accgcactgt tgacattggg atagttgaaa tgggggcaaa tcatcaaggc
     9301 gaaataaaaa cactatgcga aatagctgaa cctaactacg gaataattac caatgttgga
     9361 aaagctcact tagaaggttt tggtagtttt gaaataataa aacaaacaaa agctgaacta
     9421 tacgactatt taaaattaaa aaatggagaa atttttataa atgcagacaa tcctctatta
     9481 gttgaaatgc ttggtaatta ttccgcaata aaatatggaa tagattctaa ctatttctgt
     9541 agcggtaaat actcagaaat ttcacttcaa gctactgtaa gttggaaatc gaaaaacgaa
     9601 aaaggtatag ctaaatcaaa tttaataggt gcctacaatt ttgaaaatat tttagcagct
     9661 ataacaatag gacaatattt taaagtacca ggaactgcta ttgacaatgc aattttacga
     9721 tattatccca ataacaatag atcgcaattg gtaaaaacaa atagaaataa tttaatttta
     9781 gattattata atgcaaatcc aacgagtatg gagcttgcaa tcaaaaattt tgaaaatata
     9841 aaagataata gtaagggact tatattaggc gatatgcttg agctaggcga ggatagcgaa
     9901 attgaacaca aaaaaattct agaatatatt tcaaataaaa gttttaaaaa agtttatcta
     9961 ataggacctc atttttataa attcaaagaa gaatatggat ttaacttttt taattctgtt
    10021 gatgatttcg caaaacatat tgtaaaaaac ccagaaacag gcaacttttt cctaattaaa
    10081 ggctctagag gtgtcaaact tgaaaaatgt accgattatc tttagcaatt ttaggatatt
    10141 taccatattg actaaaatta tttaattaaa aaaactaaca attttatcgg atttatacct
    10201 attaatatat acattaattt aatgttgtga aaaaagtcta attttttaat tcattttttg
    10261 gacttttgtt tttatcctac tcataccaag ttgcgttcta ttataaaaca ttaagataag
    10321 gaaaaagtgt catag
//
LOCUS       DAOPNX010000089        10316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_7379_length_10316_cov_7.433973, whole genome shotgun sequence.
ACCESSION   DAOPNX010000089 DAOPNX010000000
VERSION     DAOPNX010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10316
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7379_length_10316_cov_7.433973"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="PKN53_09110"
     CDS             complement(<1..1520)
                     /locus_tag="PKN53_09110"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOD89147.1"
                     /translation="MLLPVNTGVIFSDLSQNPIATWLWEFENGTPDSFNGQNPPEIIY
                     NVPGYHKVKLKVANEFGNSDEIVKTKYVHVYEIADTTPTAWFTADQVIVIVGNSVNFT
                     DLSWDFPFQWKWEFEGGLPANSAAQHPQNIVYNTPGEYNVQLIVRNSQGYDTLLIENY
                     IRVIPEVGPDAPVANFTTSNRLIKRNTPVFFEDLSTNNPMFWSWYFEGGEPSSSNTQL
                     QPNGVRYQNTGFFDVTLNVGNINGSSMLTKKDYIVVYDSFVGEVCDTIGNFKQGEQPI
                     AMQINGMTGYYGGQNSDRISMYADYYDFHTFNEIRGIIVPVMKLSYGSDNAYIRFITW
                     DGADPKPTTVLSSQKVLLKTLRQNYFQVIMFDEPLEVDGPFYLGYSINYAAGDEFVVG
                     MAPNRGHGNDNTLFVYKNEEWYSSPSAYQISTSTGIRPMTCLVGIEDEEIETGITVYP
                     NPCGDNLFIVNSLSFDKGDFVEIFDRTGKMLQMKFAEAGSEIIEFNTNGIASGIYYA"
     gene            complement(1890..2066)
                     /locus_tag="PKN53_09115"
     CDS             complement(1890..2066)
                     /locus_tag="PKN53_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89148.1"
                     /translation="MKKLFCLLSLLALSYVSFAQQPTQAVDFTITDLDGVEHNLFTYL
                     DAGKHVYIEFILAG"
     gene            complement(2136..3074)
                     /locus_tag="PKN53_09120"
     CDS             complement(2136..3074)
                     /locus_tag="PKN53_09120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOD89149.1"
                     /translation="MRRKFRNLYIKFLLLIKKIGSKIILPGFKGLSLYYIGKFFFQGI
                     RKSSVIMRAAAVSFNMFISLFPALIFLFTLIPFIPIDGFQLELFKILENVLPASAYEL
                     FYETITDTIMQQNTKLLSLSILLMIYFSSNGIMSLIEAFNKTSHNIETRKSLNLRIVA
                     LISVIGISLLIMLAISLIVMSNTFILKFFSHHPEILSKGILVTLTGVVKWLIVLVLYF
                     LAISILFYFAPSKKNKFKFISPGAIFATFLQVITVLGFTFYVNNFAQYNKLYGSIGTV
                     IVVMLLIYISSLSLILGFELNASILHGDKTMKEKKV"
     gene            complement(3071..3922)
                     /gene="nadC"
                     /locus_tag="PKN53_09125"
     CDS             complement(3071..3922)
                     /gene="nadC"
                     /locus_tag="PKN53_09125"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOD89150.1"
                     /translation="MIPETYLNSLIERAYKEDVGDGDHSSLACIDASATGKAKLIAKA
                     NGVLAGVEVAKKVCQYFSPNLKTDIYINDGEKIKKGDLVLEISGFRQEILKVERTMLN
                     FMQRMSGIATETAKYAEALKPYKTKILDTRKTTPGLRLIEKEAVKIGGGKNHRIGLYD
                     MIMLKDNHIDFAGGLEKAINKTNQYLKDKNLNLKIEIEVRNIDELKKVLEVGNVDIIM
                     LDNFNIADTKLAVELIDGRYKIESSGGITFETLTDYAECGVDYISSGALTHHVKSLDL
                     SLIVEKA"
     gene            complement(3919..4320)
                     /locus_tag="PKN53_09130"
     CDS             complement(3919..4320)
                     /locus_tag="PKN53_09130"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HOD89151.1"
                     /translation="MKNLKKLVVLSFVMAFCSSVFAFPVFDIPPKIFDAMKVGNSTEL
                     TKYFNTTVELVILDKEDVYSKQQAEQILKNFFDKNKVKNFTLLHQGGKSGAQYAIGNL
                     ETEDGKTYRVYFLVKESGGKPLVHQMRIEKE"
     gene            4451..4924
                     /gene="rlmH"
                     /locus_tag="PKN53_09135"
     CDS             4451..4924
                     /gene="rlmH"
                     /locus_tag="PKN53_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOD89152.1"
                     /translation="MNILLIAVGKTDLNSISHLCDDYFKRIKRYINFEFKIIKDIKTT
                     AKTNESIQKKLEGEKILSELSSSDFVVLLDEKAKEFTSREFASFLEKQMNMSTKNLIF
                     VIGGAYGFSQEVYDRANLKFSLSQMTFSHQIIRIIFAEQLYRAFTIINNEPYHHD"
     gene            4917..8627
                     /locus_tag="PKN53_09140"
     CDS             4917..8627
                     /locus_tag="PKN53_09140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD89153.1"
                     /translation="MIKNNFPNIKIWHLIVSFSLVLIGLISQQFVSKSNTNTKYTLNK
                     EKIETILKEKNILLKGTLKQLTENYKTDAKQTQEWFYSQGKNIYNEKEIGYFLYDKNN
                     LKFWTTNDISVPTSTNISFFDNKLLNLPNGWYLCQTSSADNFFIVGLARIKKDYSYEN
                     HLIKNSFAKDFDLTHDAVISREEIQGSTKIDIDDTGSSFYLSIKTNKNINKVKFSFYN
                     FLCIFNMLNLVIFVLLFTIFLSNKIKIILLLNPIFYAILILVLRYISLKYNYTASFND
                     FRLFSPELFAESFWLQSLGDYIINVILFLSFAILVFFNYKIDLDSNFPKPIKVIFSIS
                     LASIMFLSGLFISTLMFGLVWNSSMTFSFENILALDIYSLIGTLIIFLIILSFVLIFI
                     KLFKVFKNVLNFWYLVISLFVLCSLYLIFAETTKLSFYDNLVFAFLFFLAWSSLAFVM
                     LKSKTNYFFSVGIIIIFAFLISEHFSRLSDKKEIETFKVLTYNLAAEKDVGAEFFIKE
                     SFYDLMNDEKLVEHLKNKNYDKIENAVSSEFFNNRYFNKYEIQITMCEKEDSLLIEPD
                     LVSVDCFEFFREQINEYGIIIPSTNFYFLDNHNGRISYLGEIRFQENDSTAINIYVEL
                     NSKIFSEGLGYPEILLDEKLLVKKTARSYNHAKYNKSRLITSKGDYKYPFAIASDTND
                     TAEYKLFKKDSYIHFQYRPDSDNLIIISKPYSQYSRQLVSFSYIFLFLFVVFNLMWLF
                     SKMRTANFWKGVSLKVKIQSWFIIILVVSLLITGSIAMRFIIEGYKEKQSEAMLDKLI
                     SVAVELEYSIGQKKAINYEDVHYLNNLLIKLSNVFYIDINLYDLNGILLSTSRADLFD
                     KGLKARYIDANAYSELLMKNAGTVIVDEKIDEIDYMSVYIPFRNYNNEIIAYLNLPYF
                     ARQNEFAEEISNFVLAFSNIFLFLILISVIVGVFISRQLTRPLALVQEKIRQMDINKK
                     AEKIVYTKNDELGGLIREYNKKVDELTESANKLAQSEREMAWREMAKQIAHEIKNPLT
                     PMKLSVQYLERAYDLKDENWEQTYKKVSKTLIEQIDILSAIASEFSNFAKMPASKKEV
                     IDIVEVIKNSIQLFENTEDIDFEFKENLKSKGFVYADKEQMLRVFTNLIKNGIQAIPK
                     ERMGLIKIEISSSDKNYIIKVIDNGTGISEQMIPKIFQPNFTTKSGGMGLGLSMVKSL
                     LTSNNATIYFETEENIGTIFIIEIPMYNENNF"
     gene            8706..9581
                     /locus_tag="PKN53_09145"
     CDS             8706..9581
                     /locus_tag="PKN53_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOD89154.1"
                     /translation="MLISMTGFGRASTEYDGKKIIIDIKTLNSKNIDITTRIPNAYRE
                     KEIEVRKLLSNELQRGKIEFSIFVEANGGETNAKLNDKIILDYYNQLKNLCQSNNIPF
                     SNDVLSSIIRMPDVVATTIEELNEEEWEHILKFINQAIEDCKNFRKHEGSILTQDIIT
                     NLNSITNLLNQVPQYEEERIDIIKDRLSISLNEIVDTANIDANRYEQEIIFYLEKLDI
                     NEEKVRLKKHCDYFLETLNTEIYSGKKLGFIAQEMGREINTLGSKANHVEIQKLVIMM
                     KDELEKIKEQVLNIL"
     gene            9584..10162
                     /gene="gmk"
                     /locus_tag="PKN53_09150"
     CDS             9584..10162
                     /gene="gmk"
                     /locus_tag="PKN53_09150"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008776350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOD89155.1"
                     /translation="MKPKVLIFSAPSGSGKTTIVNHLLSNPKFNLSFSISACTRKPRG
                     VEEHGKHYYFLSAEEFKQKIANDEFLEWEEVYANTFYGSLKSEVDRLTKLGKVVVFDV
                     DVKGGINIKNFYGENALSVFIMPPSIEELEQRLIKRSTDQPEVIKTRIEKAKYEMSFA
                     KEFDIILTNNELQTALIEAEKIVEKFLQGKSL"
BASE COUNT         3809 a         1554 c         1434 g         3519 t
ORIGIN      
        1 gcgtaataaa ttcctgaagc aattccgtta gtgttaaatt caattatttc gcttccagct
       61 tcagcaaatt tcatttgtag catttttcct gttctgtcaa aaatttcaac aaaatcgcct
      121 ttatcgaaag acaatgagtt tacgataaac aaattatctc cacatggatt tggataaact
      181 gttattccag tttcaatttc ttcgtcttca attccaacaa ggcatgtcat tggtcttatt
      241 cctgtagatg ttgaaatttg ataagcagaa ggcgaagaat accattcttc gtttttatat
      301 acaaataaag tattatcgtt gccatgtcct cgatttggag ccatacccac tacaaattca
      361 tcgccagcag cataatttat agaatatccc aaataaaatg gaccatcaac ttcaagaggc
      421 tcatcaaaca ttattacttg aaaataattt tgtcgcaaag ttttcaaaag cactttttga
      481 cttgataaaa cagttgtagg ttttgggtct gcaccatccc aagtaataaa tctaatataa
      541 gcattatccg aaccatatga caatttcatt acaggaacaa taattcctct tatctcatta
      601 aaagtatgaa aatcataata atcggcatac atgctaattc tatcactatt ttgtccgcca
      661 taataacctg tcattccatt tatttgcata gctattggtt gttcgccttg tttaaaatta
      721 cctattgtat cgcaaacttc gccaacaaaa gagtcgtaaa caacaatata atctttcttg
      781 gttaacatcg aagaaccatt aatatttcca acatttagag taacgtcaaa aaagcctgtg
      841 ttttgataac gaactccatt tggttgtaat tgagtattag agcttgatgg ttctcctcct
      901 tcaaaatacc aagaccaaaa cattggatta tttgttgata aatcctcaaa aaagactgga
      961 gtattacgtt taatcaaacg attagaagtt gtaaaattgg caacaggagc atctggacca
     1021 acttctggaa taactctaat gtaattttca attaacaaag tatcatagcc ttgagagtta
     1081 cgaacaatta actgaacatt atattcacct ggtgtattat aaacaatatt ttgaggatgc
     1141 tgtgctgcag aatttgcagg caaaccacct tcaaactccc atttccattg gaatggaaaa
     1201 tcccaactta ggtctgtaaa gtttacagaa ttaccaacaa taacaattac ttgatcagca
     1261 gtaaaccaag ctgttggagt agtatcagca atctcataaa catgaacata tttagtttta
     1321 actatctcat cactatttcc aaattcattt gcaactttaa gttttacctt atgataacca
     1381 ggaacattat aaataatttc aggtggattt tgaccattaa aactatctgg agtgccgttt
     1441 tcaaactccc aaagccaagt tgcaatagga ttttgcgata agtcagaaaa aataactcct
     1501 gtatttacag gaagcaacaa agggtcgcca gaaaaatcag caatgggcca atcttctgga
     1561 caagcatttc tgttaattcc aatttcttga agaccttgaa cagtatttat atcaacttct
     1621 tggtcatcat cccaaataac acttctatct ggtttaatta aaattttagt tggataagct
     1681 tctattccat aatagttaac gacagcacct ccatgcccct caacagcact acaaagaggg
     1741 aaaatagtat tatgttcatc tctataatta atagctattt cattggttac accctgagaa
     1801 aaaagaccaa gaactactaa ttcaaattca ttacaaccaa aacttgaata tatattattt
     1861 atcgtcggga cagacgcaat acagccaggt caaccagcta atataaattc tatataaaca
     1921 tgtttacctg catctaaata agtgaaaaga ttatgctcaa caccatcgag atctgttata
     1981 gtaaaatcca cagcttgagt tggttgttgc gcaaaactta cgtatgacaa ggctaacaaa
     2041 gataataaac aaaataactt tttcatatta ataaaaattt taccattatt tgcaattttc
     2101 aaagatagta aattaattaa tataaacgtt ttgaattaaa cttttttttc tttcatagtt
     2161 ttgtctccat gtaaaatact tgcatttagc tcaaatccaa gaattaaaga taaagagcta
     2221 atataaatta aaagcattac aactattaca gttccgatag agccataaag tttattatac
     2281 tgtgcaaaat tatttacata aaatgtaaaa cctaatacag ttataacttg taaaaaagtt
     2341 gcaaaaatag caccaggact tataaatttg aacttgttct tttttgaagg agcaaaataa
     2401 aacaaaatag aaattgccaa aaagtataaa accaagacta tcaaccactt aaccacacca
     2461 gtcaaagtta ctaatatacc ttttgataaa atttctgggt gatgagaaaa aaactttaat
     2521 ataaaagtat tactcatcac aatcaaactt attgcgagca ttatcaacaa gctgattcca
     2581 atcacagaaa ttaaagctac tattctaaga ttaagagatt tacgagtttc tatattatga
     2641 gaggttttgt taaaagcttc tataagagac attattccat ttgaggagaa atatatcatc
     2701 aataaaatac ttagagaaag aagttttgta ttttgctgca tgattgtatc tgtaatagtt
     2761 tcataaaaca attcgtatgc tgatgctggt aaaacattct ctaaaatctt aaaaagctct
     2821 aattgaaaac catctattgg aataaaagga attaaagtaa acaaaaaaat caatgctggg
     2881 aataatgata taaacatatt aaacgaaacc gcggctgctc gcattattac agaacttttt
     2941 cttatccctt gaaagaaaaa ttttcctata taatataaag acaagccttt aaatcccggc
     3001 agtataattt tgctcccaat ctttttgatt aataacaaaa acttaatgta taaattacgg
     3061 aattttcttc tcacgctttt tctacaatta aacttaggtc taaacttttt acatgatgag
     3121 taagagctcc tgatgaaata taatcaacac cacattctgc ataatctgta agcgtttcaa
     3181 aagtaattcc acccgaagat tcaattttat atctaccgtc tataagttct actgcaagtt
     3241 ttgtgtctgc aatattaaag ttgtctaaca ttattatgtc aacattgcca acttctagca
     3301 cttttttaag ctcatcaata tttcttactt ctatctcaat ttttaggttt aaatttttgt
     3361 cctttaaata ttgatttgtc ttgttaattg ctttttccaa acctcctgca aaatcaatat
     3421 gattatcttt aagcataatc atatcataaa gacctattct atgatttttg ccaccgccaa
     3481 ttttaacggc ttctttttca attaatctta atccaggagt tgttttgcgt gtatctaaaa
     3541 tttttgtttt ataaggtttt aatgcttctg catatttggc ggtttcggtt gcaattccgc
     3601 tcattctttg cataaaatta agcattgttc tctcaacttt aagaatctct tggcgaaaac
     3661 cagaaatttc taaaactaga tctccctttt ttattttttc tccgtcatta atataaatat
     3721 cggttttcaa atttggagaa aaatattggc aaactttttt agcaacttct actcctgcca
     3781 aaacgccatt tgctttagca ataagtttag ctttaccagt tgccgaagca tcaatacaag
     3841 ccaaagaaga gtggtcgcca tcgccaacat cttctttata ggctctttct attaaagagt
     3901 ttaaatatgt ttcagggatc attctttttc tatacgcatt tgatgtacca aaggtttgcc
     3961 accactttct ttcacaagaa aatacactct ataagttttt ccatcttcgg tttcaagatt
     4021 tccaattgcg tattgagctc cagattttcc tccttgatgt aaaagcgtaa agtttttaac
     4081 tttattttta tcaaaaaagt tttttaatat ttgttctgcc tgctgtttac tataaacatc
     4141 ttctttatca agaattacta attcaacagt agtgttaaaa tattttgtca attctgttga
     4201 atttccaact ttcattgcat caaagatttt tggcggaata tcaaaaaccg gaaaagcaaa
     4261 aacacttgag caaaaagcca tgacaaaaga taaaacaact aattttttta aatttttcat
     4321 tatttattaa attaaaaagt ttttaaatac tgtaatctta ctacaaatac cgaaccaaaa
     4381 aatttcaagt cgcaagataa taataatttc attcttttaa tatatcttta tcacaaattt
     4441 ttaattcatt atgaacattt tattaattgc agtcggtaaa acagacctta actcaatttc
     4501 acacttatgc gacgactatt ttaaacgcat aaaaagatat ataaattttg aatttaaaat
     4561 tattaaagac attaaaacta ctgccaaaac aaatgagtct atacagaaaa aacttgaagg
     4621 cgaaaaaata ttatcagaat tatcaagtag cgattttgtt gtgcttttag acgaaaaagc
     4681 taaagaattt acaagtcgtg aatttgcttc ttttcttgaa aaacaaatga atatgtcaac
     4741 aaaaaatctt atttttgtaa ttggtggcgc ctatggtttt tcacaagaag tgtatgatag
     4801 agctaattta aaattttctt tatcgcaaat gaccttttcg catcaaataa ttagaattat
     4861 atttgcagaa caactttata gagcttttac aataattaat aacgaaccat accatcatga
     4921 ttaaaaataa ttttccaaat ataaaaatat ggcatctaat agtttcgttt agcttagttt
     4981 taataggttt gattagtcaa caatttgtat caaaatcaaa cactaacact aaatacacct
     5041 taaataaaga aaaaattgaa accattttaa aagaaaaaaa cattctgctt aaaggaactt
     5101 taaaacaact aacagaaaat tacaaaaccg atgctaagca aacacaagaa tggttctatt
     5161 ctcaagggaa aaacatatat aatgaaaaag aaattggata ttttttatat gacaaaaaca
     5221 atttaaaatt ttggacaaca aatgatattt ctgttcccac ttcgactaat ataagttttt
     5281 tcgacaacaa acttcttaac ctacctaatg gttggtattt gtgccaaaca agtagtgcag
     5341 acaacttttt tattgttgga ctagcaagaa taaaaaaaga ttattcctat gaaaaccatt
     5401 tgattaaaaa cagttttgcc aaagattttg atttaaccca tgacgctgta attagtagag
     5461 aagaaattca aggaagtaca aaaattgata ttgacgacac aggaagtagt ttttatttgt
     5521 ctattaaaac taacaaaaac ataaataaag ttaagtttag cttttacaat tttctttgta
     5581 ttttcaacat gcttaaccta gtaatttttg ttttactttt cacaatattt ctttcaaaca
     5641 aaattaagat aattttattg ctcaatccta ttttttacgc aatacttata cttgtcttac
     5701 gatatatttc attaaaatat aattataccg ctagttttaa tgattttaga ctttttagcc
     5761 ccgaactttt tgctgaatcg ttctggttgc aatctttagg agattatata ataaatgtta
     5821 ttctcttttt aagttttgct attttagtat tttttaatta taaaattgat ttagattcta
     5881 atttcccaaa accaattaaa gtaatttttt cgatttcact tgctagtata atgtttttaa
     5941 gtggtttgtt tatttcaact ttaatgtttg gtttagtttg gaactcttct atgacttttt
     6001 catttgaaaa cattttagct ttagatattt atagcttgat tggcacttta ataatctttt
     6061 tgataatctt atcttttgtt ttaattttta taaaattatt taaggttttt aaaaacgtat
     6121 taaatttttg gtatcttgtt attagtctgt ttgttttgtg ctctctatat ttaatatttg
     6181 cagaaacaac aaaattgagc ttttacgata atttagtttt cgcttttctg tttttccttg
     6241 catggtcgag cttagcattt gtaatgttaa aatcaaaaac caactatttt tttagtgttg
     6301 ggataattat aatttttgct tttttaattt cagaacactt ttcacgcctt agcgacaaaa
     6361 aagaaataga aacttttaaa gtattaacct ataaccttgc tgcagaaaaa gatgttggtg
     6421 ccgaattttt tattaaagaa tctttttacg atttaatgaa tgatgaaaaa ttagttgaac
     6481 atctaaaaaa taaaaattat gacaaaattg aaaacgcagt aagctctgaa ttctttaaca
     6541 atagatattt taataagtat gaaattcaaa ttacaatgtg cgaaaaagaa gatagtctgt
     6601 tgattgaacc agacttagtt tcggtagatt gttttgaatt ttttagagaa caaatcaacg
     6661 agtatggaat aattattcct tcaacaaatt tttatttctt agataaccac aatggtcgaa
     6721 tatcttatct tggagaaatt agatttcaag aaaatgatag cactgctata aatatttatg
     6781 tagaattaaa ttcaaaaata tttagcgagg gcttaggtta tcccgaaata ttattagacg
     6841 aaaaactatt ggttaaaaaa actgcaagaa gctacaatca cgcaaaatac aacaagtcta
     6901 gactaatcac cagcaaagga gactataagt atccgtttgc tattgcttct gacacaaacg
     6961 atactgccga atataaatta tttaaaaaag attcttatat tcattttcaa tatcgtcccg
     7021 actcagacaa tttaattata ataagcaaac cttactctca atattcaaga caattagtat
     7081 cgttttcata tattttctta tttctttttg ttgtcttcaa tttaatgtgg ttgttttcta
     7141 aaatgagaac agcaaacttt tggaaaggtg taagtttaaa agttaaaatt caatcttggt
     7201 ttataattat tctagtagtt tctttattga taacaggctc aatagctatg agatttatta
     7261 ttgaaggata taaagaaaaa caaagtgaag ccatgttgga taaattgatt tctgttgctg
     7321 ttgagttaga atatagtatt ggacagaaaa aagctataaa ttacgaagat gttcattacc
     7381 taaacaactt attaattaaa ttgtctaatg ttttttacat agacataaat ctttacgatt
     7441 tgaatggaat tttgctttct acctcgcgag ccgacttgtt tgacaaaggt ttaaaagcta
     7501 gatatattga tgctaatgcc tattcggagc tattgatgaa aaatgctgga actgttattg
     7561 ttgacgaaaa aattgatgaa atagattata tgtcggttta cattcctttt agaaactaca
     7621 acaacgaaat aatagcttat ttaaatcttc catattttgc tcgccagaac gagtttgctg
     7681 aagaaatttc taattttgtt ttagcattct cgaatatatt cttgttttta attttaatat
     7741 ctgtaattgt tggagtgttt atttcgcgac aattaactcg tccgcttgct ttggtgcaag
     7801 aaaaaattag acaaatggat attaataaaa aagcagaaaa aattgtttat actaaaaatg
     7861 acgaattagg tggattaata cgtgaataca ataaaaaagt tgatgaatta acagaatcag
     7921 ccaacaaact tgctcagtct gaaagagaaa tggcatggcg tgaaatggca aaacaaatcg
     7981 ctcacgaaat taaaaacccg cttacaccaa tgaagctgag cgtacaatac ttagagcgtg
     8041 cttatgattt aaaagatgaa aattgggaac aaacatataa aaaagtgtct aaaactctta
     8101 tcgaacaaat agatatttta tctgcaattg catcagagtt ttcaaatttt gcaaaaatgc
     8161 ctgcttctaa aaaagaagtt attgatattg ttgaagttat taaaaactca attcaactat
     8221 ttgaaaacac tgaagatata gattttgaat ttaaagaaaa tctaaaatct aaaggttttg
     8281 tttatgcaga taaagagcag atgcttagag tttttaccaa tttaattaaa aatggtattc
     8341 aagcaattcc aaaagaaaga atgggtttga taaaaataga aatttcaagc tcagataaaa
     8401 attacattat taaagttatt gacaacggaa caggaatttc ggaacaaatg attcctaaaa
     8461 ttttccaacc taatttcaca acaaaatctg gaggaatggg attagggcta agcatggtaa
     8521 aaagtttgct aacaagcaat aatgcaacca tttatttcga aacagaagaa aatattggaa
     8581 caatattcat catagaaatt ccaatgtata acgaaaacaa cttttaaaat gcgtatattt
     8641 tttaaaataa attgtatttt ctcgctttaa agtctaattt tgtaagcaat aaaataataa
     8701 tagttatgct aatttcgatg acaggctttg gtagagcttc taccgaatat gatggtaaaa
     8761 aaattatcat agacattaaa actcttaact ctaaaaacat tgatataact acacgtattc
     8821 caaatgctta tagagaaaaa gaaattgaag taagaaaatt attgtctaac gaacttcaaa
     8881 gaggtaaaat agaattttct atatttgttg aagcaaacgg aggagagact aatgcaaagc
     8941 ttaacgacaa aataatttta gattattaca atcaattaaa aaatttgtgc cagtctaaca
     9001 atattccttt ttcaaatgat gtgttatcgt ctattattag aatgcctgat gtagttgcaa
     9061 caactataga agaattaaac gaagaagaat gggagcatat tttaaaattt ataaaccaag
     9121 ctattgaaga ttgtaaaaat tttagaaaac atgaaggctc tattttgaca caagatataa
     9181 ttactaattt gaactctatt actaatcttt taaatcaagt tccgcaatac gaagaagaaa
     9241 gaatagatat tataaaagac aggctaagca taagtttgaa cgaaattgtt gacactgcaa
     9301 atattgatgc taacagatat gagcaagaga taatttttta tttagaaaaa ctcgatatta
     9361 acgaagaaaa agtaagatta aaaaagcact gcgattattt tttagaaact ttaaatactg
     9421 aaatatattc tggtaaaaaa ttagggttta tagctcaaga aatgggaagg gaaataaata
     9481 ctttaggttc aaaagccaac catgttgaga ttcaaaagtt agtaattatg atgaaagatg
     9541 agttagaaaa aattaaagaa caagttttaa atattttata aatatgaaac caaaagtttt
     9601 aatattttcg gcacctagtg gttcaggaaa gacaacaata gtaaatcatt tgctttctaa
     9661 tccaaaattt aatcttagtt tttctatttc tgcctgcact cgcaaaccca gaggcgttga
     9721 agaacatggc aaacattatt attttttatc agcagaagaa tttaagcaaa aaatagcaaa
     9781 tgatgagttt ttagaatggg aagaagttta tgcaaatact ttttacggca gtttaaaatc
     9841 agaagttgac aggcttacaa aactaggaaa agttgttgtc tttgatgttg atgtaaaagg
     9901 tggaataaat attaaaaatt tttatggaga aaatgccttg tcggttttta ttatgccacc
     9961 aagcattgag gaattagaac aaagactgat aaaacgttct accgaccaac ccgaagttat
    10021 taaaacaaga attgaaaaag caaaatacga aatgagcttt gcaaaagaat ttgatataat
    10081 cctaactaac aacgaattac aaacagctct tatagaagca gaaaaaattg ttgagaaatt
    10141 tttgcagggt aaaagtttgt aaagttgaat tagttaccat tcgctcgatt ttccccacac
    10201 aatcctattt cagattttcc tgcacaaccc gctcgctcgt ttcaacttta cgaactttat
    10261 gaacttcctt aaatccgcaa ctaaaaatat ttattgcaat tttagataaa attatt
//
LOCUS       DAOPNX010000090        10292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_7399_length_10292_cov_6.283188, whole genome shotgun sequence.
ACCESSION   DAOPNX010000090 DAOPNX010000000
VERSION     DAOPNX010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10292
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7399_length_10292_cov_6.283188"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2046
                     /locus_tag="PKN53_09155"
     CDS             <1..2046
                     /locus_tag="PKN53_09155"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOD89156.1"
                     /translation="VASPTITYNTPGTYNVTLTITAGSQNATETKNGYITVVQSGGSG
                     GFTYDFEACTNFAVDQFSPCTTYDGDGNQTYGIQNVSFENQNYTGSFITFNSSATNPS
                     LAGGNWDAHGGSKCGACFSATTPPNNDWFITPKIDLVSNSSFSFWAKTANTNYDKERF
                     NVLISTTNNNISSFTKISTGTYTEAPTTWTKYTYDLNAYNGQGVYLAIQCVSHDAHAF
                     LIDDIEILTSSGGSQNLVADFSGTPTTVTAGNTVSFTDLSTGGTITSRSWSFPGGSPS
                     SSTAASPTITYNTPGTYNVSLTVSNGSQNATETKNGYITVVQSGINGGFTYDFEACTN
                     FAVDQFSPCTTHDGDGSQTYGIQNVEFENAGYIGAFIAFNASATNPSLAGGNWNAHGG
                     SKYGACFAATTPPNNDWFITPKINLESNSSFSFWAKSITEEHGKERFNVLISTTNNNI
                     SSFTKISTGTYTEAPATWTKYTYDLNAYNGQGVYLAIQCVSNDAFVFMIDDIEILTSS
                     GGTVTAPVANFTANPTSGCAPLTVSFSDQSSNNPTSWSWSFNGALPSSSVSPNPTVIY
                     NTPGIYSVTLTASNAGGSNTKTMTNYITVNDCSPVNDNLISKISIYPNPTDGIVNIDI
                     PESKATVRISDILGKVVKVANLSSNQSSIDLSTLKSGMYFIEIQLSEAKYTSKLTIQ"
     gene            2487..3935
                     /gene="sufB"
                     /locus_tag="PKN53_09160"
     CDS             2487..3935
                     /gene="sufB"
                     /locus_tag="PKN53_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOD89157.1"
                     /translation="MEKEQDKVLREVTESDYKYGFVTDIDADEIPKGLTHETIRLISK
                     KKNEPDWMLEFRLKAFEKWLTMKMPNWAHLNIPEIDYQDIIYYSAPKQEPKYTSLDEI
                     DPEIKATFDKLGIPLDEQKRLSGVAVDVVMDSVSVKTTFQETLAEKGIIFCSFSDAVQ
                     NHPDLVKKYMGSVVPYGDNFFAALNSAVFSDGSFCYIPKGVRCPMELSTYFRINAMNT
                     GQFERTLIIADEGSYVSYMEGCTAPIRDENQLHAAIVEIIAMKDAEVKYSTVQNWYPG
                     NKEGQGGVYNFVTKRAICQGENSKVSWAQVETGSAITWKYPSTILKGDNSASEFYSVA
                     VTNNFQQADTGTKMIHLGKNTSSIIVSKGISAGKSENSYRGLVKVGKNAENARNFSQC
                     DSLLLGDKCGAHTFPYIDVDNDTAVVEHEATTSKISEDQIFYCNQRGISTEDAIGLIV
                     NGYAKEVINKLPMEFAVEAQKLLQISLEGSVG"
     gene            3978..4730
                     /gene="sufC"
                     /locus_tag="PKN53_09165"
     CDS             3978..4730
                     /gene="sufC"
                     /locus_tag="PKN53_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOD89158.1"
                     /translation="MLVIKNLHAEVSGKKILKGLSLEVKPGEIHAIMGPNGSGKSTLS
                     AVLTGNENYIVTEGEIYYEGKNLFDLNVEERAKAGIFLSFQYPVEIPGVSMINFMKTA
                     VNEQRKFKNLPPLSSGEFLKYMREMKDLVELKSDLANRSVNVGFSGGEKKRNEIFQMA
                     MLKPKLAILDETDSGLDIDALRVVANGVNKLKTPENSAIVITHYQRLLDFIVPDFVHV
                     LYDGKIVKSAGKELALELEEKGYSWIKEELGE"
     gene            4742..6106
                     /gene="sufD"
                     /locus_tag="PKN53_09170"
     CDS             4742..6106
                     /gene="sufD"
                     /locus_tag="PKN53_09170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOD89159.1"
                     /translation="MDIQKTYEKYINVFEEFKDKLKNNESEKLSNYRKKAFEIFQKLG
                     IPDNKNENYRNAGIADILNKDYEFTIDSRGAVVNLNEFFKCEVENLDTHVVLLSNGEY
                     YKDNEPITDLNEGVIICGLQEAKAKYPEIIEKYYNQYPSEIEDGFVALNTMFAQDGVF
                     IYVPDNIEVNKTIQLVNLTHGFGNKNIFKRNLFVLGKNSKLSLVICDHTLNMSNNFVV
                     DITESYVGENSQLDYHTIQNEPNKSGVINSHFINLEKYSRCNSFVISLHGGILRNNVF
                     AKLAGENSEANLYGLNLTDKDQRVDNFTLIDHVVPNCMSNELYKGILDEHAQGSFLGR
                     ILVRKNAQKTEAYQTNNNLCLTPQTKMRTRPQLEIYADDVKCSHGATVGQLGQDALMY
                     LRQRGIDAREAKLMLMFAFANDVLLKVNIPPLRERIANLLDSRLRGEFSFCDHCLLNC
                     KSRS"
     gene            6203..8554
                     /gene="feoB"
                     /locus_tag="PKN53_09175"
     CDS             6203..8554
                     /gene="feoB"
                     /locus_tag="PKN53_09175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOD89160.1"
                     /translation="MVAQQNIELNKEYTVSKITGRWGMRRRISKLGISPNTELIFTKE
                     SKSGFYKFNFVGCKRKKRLPANYLRFIKLVESDNPQLQEILAEDHAEIVIDVFSNEET
                     SDTNIFENQTEYKFKGYNFSINYLPEISTIYGDDYPRKRLRNKVLTDFPDVFLGIVDF
                     ENLEADLNLVTQFIDMDAKIVLALRNYNPESGSYKADFDLLSKLLGLPIINYSELEEE
                     NSAREKILETLIKTHQNTEPYVRHIYINYGRQVEQSISKLKKLLKRSSKADYVASPRY
                     LAIGLLEKDNLIYRLFDGLPCQNKIRRKANYEIKRLENLHNNHVFNIVKNARKAYVNG
                     AITEITSGKKTDTRSEKLDKILTHKFWGIPIFLAFMGLTFFATFELGKYPMEWLENGI
                     DSLSKYLANVMKPGFFKDLVLDGAIGGVGGVLVFLPNIFILFFFIGILENTGYMSRAA
                     FLMDKYMHKIGLHGKSFIPMIMGFGCTVPAIMSTKILENRRDRILTMMILPFMSCSAK
                     LPVYILMISAFFPEHPVLILFGIYLFGIILALVVSKFFSKTILKQKEAPFVMELLPYR
                     KPTAKLLLVYTWDRGKEYLKKIAGVILIGSIIIWLLGYFPKNIDYSKDYESLIAKEQK
                     QEQPNAEKIHELEFQKRSEEHEKSYIGKIGHFVAPVMEPLGFDWKMSVSILAGIAGKE
                     ITVSTMGVLYQADEDLESGSLQNKLKAETYAFGEKAGEKVYNKAVALAFILFILIYFP
                     CIATISAIWKESKLKWAIFSGIYTTAIAWVVGFAAYQIAKYFI"
     gene            8571..9953
                     /locus_tag="PKN53_09180"
     CDS             8571..9953
                     /locus_tag="PKN53_09180"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOD89161.1"
                     /translation="MKKLIFYVFLGISINAFSQNSNLTYLQGNIIANQDSIEIIISID
                     ENNSAYLFVPEQFLYKMESNDTFYGGDSLFCNFKKIPASFCGKLIDETQTFSGVWKQG
                     KSELKTELKITDNSEFAFLNRPQTPEPPFPYLEKNICVENLKGNSVLCGSLTIPDTLK
                     SYPLVILVSGSGAQDRNEEIAGHKTFMVIADYLTQNGIAVFRYDDRGIAESKGDFANS
                     TTYDFMTDTEAVLNYFYDFPNIDKTNIGVLGHSEGGLIAFMLAAKETKKLNFIISLAG
                     PGVPIKELLVQQLKDVNKSFNFPDEHIEVMCEMQSEILDIATKNVDMYELRKNIKIIY
                     DKYAQHFTEEERQKYQIGDAAINVAVMQLSSPWMKYFIKIDPGKYIKKVKCRVLSING
                     STDLQVKMEDNLNSIEKNLKTKKEVQIVAIEGLNHLFQKSETGAISEYYTIQNTIEQL
                     VLDKIVEFIK"
BASE COUNT         3692 a         1405 c         1814 g         3381 t
ORIGIN      
        1 gttgcttcac caacaattac ctacaatact ccaggaactt ataatgtaac tttaactatt
       61 accgctggtt cacaaaatgc aacagaaaca aaaaatggat atattactgt tgttcagtca
      121 ggtggtagcg gtggttttac ttatgatttt gaagcttgta caaattttgc agttgaccaa
      181 ttttcacctt gcacaactta tgatggagat ggaaaccaaa cttacggtat tcaaaatgtt
      241 tcatttgaaa accaaaatta tacaggttct tttataactt ttaattcttc agcaacaaat
      301 ccttctcttg ctggaggaaa ctgggatgca catggtggaa gtaaatgcgg tgcttgtttt
      361 tctgcgacaa cgcctcctaa caatgattgg tttattactc ctaaaataga tttagtttcg
      421 aattctagtt ttagcttttg ggcgaaaaca gctaatacta attatgacaa agaaagattt
      481 aatgtattaa tttctacaac aaataataat atttcaagtt ttacaaaaat ttctacagga
      541 acttatacag aagctccaac aacttggaca aaatacactt atgatttgaa tgcttataac
      601 ggacaagggg tttatctagc tattcaatgt gtctctcatg atgctcatgc atttttgata
      661 gatgatattg aaattttaac ttcttctggt ggatctcaaa atcttgttgc tgatttttca
      721 ggaacaccaa caactgtaac tgcaggtaat actgttagtt ttactgattt gtcaacagga
      781 ggaacaataa caagtcgttc gtggtctttc cctggtggaa gtccttcaag ttctacggca
      841 gcttcaccaa caatcactta taatactcca ggaacttata atgtaagttt aactgttagt
      901 aatggttcac aaaatgcaac agaaacaaaa aatggatata ttaccgttgt tcagtcaggt
      961 attaacggtg gctttactta tgattttgag gcttgtacaa actttgcagt agatcaattt
     1021 agcccttgca caactcatga cggagatgga agtcaaactt atggtattca aaatgtagag
     1081 tttgaaaatg caggttatat aggtgctttt attgctttta atgcttctgc aacaaatcct
     1141 tcccttgctg gaggaaattg gaatgcacat ggaggaagta aatatggtgc ttgtttcgct
     1201 gcaacaacgc ctcctaacaa tgattggttt ataacaccta aaataaattt agaatcaaat
     1261 tctagtttta gtttttgggc aaaatcaatt acagaagaac atggcaaaga aagatttaat
     1321 gttttaattt ctacaacaaa taataatatt tcaagtttta caaaaatttc tacaggaact
     1381 tatacagaag ctccagcaac ttggacaaaa tatacttatg atttgaatgc ctataatgga
     1441 caaggagttt atctagctat tcaatgtgtt tctaatgatg cttttgtatt tatgatagat
     1501 gatattgaaa ttttaacttc atcaggaggt acagttacag ctcctgtcgc taattttaca
     1561 gcaaatccaa cttcgggatg tgctccatta acagtaagtt tctctgatca atcaagcaat
     1621 aatccaactt catggtcttg gagttttaat ggtgctttgc caagttctag cgtttcgcca
     1681 aatcctactg ttatatataa cactccagga atatactctg ttactttaac agcaagcaat
     1741 gctggcggta gcaatacaaa aacaatgaca aactacatta cagtaaacga ttgctcacct
     1801 gttaatgata atcttatttc taagatttct atttatccta atccaacaga tggaattgtt
     1861 aatatagata ttccagaaag taaagcaaca gttagaataa gtgatatttt aggtaaagtt
     1921 gttaaagttg caaacttgag ttcaaatcaa tcaagtatag atttaagcac tttaaagtct
     1981 ggaatgtatt ttatagaaat tcagttgtct gaagcaaaat atacttcaaa gctaactata
     2041 caataatact cagtttaaag tttttaaagt ttgtaaagtt tttaaagttc ataaagtcga
     2101 accgagttta cgagtttacc gaaggtaaag tggctgacgc gtctattgct tcgcagagtt
     2161 cgcaagctca ctctgctcgc tttgatgcta gttgtccgaa ggacaacaca agaccatcaa
     2221 aagattgtcc gtaggacaac atgtgattaa ccgtaggcgc agcctacggt agttgtcccc
     2281 tcaaaggctt tgcccgcaag ggcaacacaa taaacacggt tgaaagagtt tttatttatt
     2341 aatattgctt tgaaattttt taaattattt tttcttccaa acatttctat tttaatattg
     2401 ttattaaata ggtgggcgta taaaaatatt aaaaatatta attgtatttt tgcaaattat
     2461 taaaattgaa actttaattg tttagaatgg aaaaagaaca agataaagtg ttgcgagaag
     2521 taaccgaaag cgattataag tatggttttg ttactgatat agatgcagat gaaattccta
     2581 aaggacttac tcacgaaact attcgcttga tttctaaaaa gaaaaacgag cctgattgga
     2641 tgttggaatt tagacttaaa gcttttgaaa agtggttgac aatgaaaatg ccaaattggg
     2701 cacatttgaa tattccagaa attgattatc aagatattat ttactattca gcacctaaac
     2761 aagaaccaaa atatactagt ttagatgaaa ttgaccccga aattaaagct acttttgata
     2821 aattaggcat tcctttagat gagcagaaac gtttgtcagg agtggctgta gatgtggtaa
     2881 tggattcggt ttcggttaag actacttttc aagaaacatt agctgaaaaa ggaataattt
     2941 tctgctcatt ttctgatgct gtgcaaaatc accctgattt agttaaaaaa tatatgggtt
     3001 ctgttgtacc ttatggagat aatttttttg ctgctttaaa ctcagctgtt tttagcgatg
     3061 gctcgttttg ttatattcca aaaggtgtac gctgtccaat ggaattatca acttatttta
     3121 gaataaatgc tatgaacact ggacagtttg aaaggacttt gattattgct gacgaaggtt
     3181 cttatgtaag ctatatggaa ggttgtacag caccaatacg tgacgaaaat caacttcatg
     3241 cagctatagt tgaaattatt gcaatgaaag atgctgaagt aaaatattca acagtgcaaa
     3301 attggtatcc aggaaataaa gaggggcaag gtggtgttta taattttgta acaaaacgtg
     3361 caatttgtca aggagaaaat tcaaaagtta gctgggctca agtcgaaact ggctcggcaa
     3421 ttacttggaa atatcctagc acaatcttaa aaggcgataa ttctgcttca gaattttatt
     3481 cagtagcagt tacaaataat tttcaacaag ctgatactgg aacaaaaatg atacatcttg
     3541 gaaaaaatac aagctctata attgtatcta aaggtatttc ggctggaaaa agcgaaaatt
     3601 cttatcgtgg attggtgaaa gttgggaaaa atgcagaaaa tgcaagaaat ttctcacaat
     3661 gcgactcttt acttttagga gataaatgtg gtgctcatac ttttccttat attgatgtag
     3721 ataacgatac agcagttgta gaacacgaag ctactacatc aaaaattagt gaagaccaaa
     3781 ttttttattg caatcaaaga ggaatttcaa ccgaagatgc aataggactg attgtaaatg
     3841 gttatgccaa agaagttata aataagctac caatggaatt tgcggtagaa gcacaaaaat
     3901 tattacaaat tagtttggaa ggaagtgttg gataaaattt taaaaataaa ttaaataata
     3961 ataaaaaaac atataatatg ttagttatta agaacttaca tgcagaagtg agcggaaaga
     4021 aaattttaaa aggattaagt cttgaagtta aacctggcga aattcacgct ataatgggac
     4081 ctaatggttc aggaaaatca actttatcgg ctgtgcttac aggtaacgaa aactatattg
     4141 ttaccgaagg tgaaatttat tacgaaggga aaaatttgtt cgatttgaat gttgaagaaa
     4201 gagcaaaagc tggaattttt ctaagttttc aatatcctgt cgaaattccc ggtgtaagta
     4261 tgattaactt tatgaaaaca gcagttaatg aacagcgtaa atttaaaaat ttaccaccat
     4321 tgtcaagtgg cgagttttta aaatatatgc gtgaaatgaa agaccttgta gaactaaaaa
     4381 gcgaccttgc caatcgttcg gtaaatgttg gtttttcggg aggcgaaaaa aagagaaatg
     4441 aaatttttca aatggctatg ttaaaaccaa aactcgcaat tcttgacgaa acagactcag
     4501 gacttgatat tgatgctctt agagttgtgg ctaatggagt taataaactt aaaactccag
     4561 aaaattccgc tatagttatc actcactatc aacgtctgtt agactttatt gtacctgatt
     4621 ttgttcatgt cttgtatgat ggaaaaattg taaaatcggc aggcaaagaa cttgctcttg
     4681 agttagaaga aaaaggttat agctggataa aagaagaatt aggagaataa aacaaactat
     4741 tatggatatt caaaaaactt acgaaaaata tataaatgtt tttgaagaat ttaaggataa
     4801 attaaaaaat aatgagtccg aaaagctttc taattataga aaaaaggctt ttgaaatttt
     4861 tcaaaaactt ggaatacctg ataataaaaa cgagaattat agaaatgctg gtatagctga
     4921 tattcttaat aaagattatg agtttaccat agattcgcgt ggtgctgttg tgaatttgaa
     4981 tgagtttttt aaatgtgaag tagaaaattt agacactcat gttgtacttt tatctaacgg
     5041 cgaatattat aaagataatg aaccaattac agatttgaat gaaggagtga taatctgtgg
     5101 tttacaagag gcaaaagcaa aatatcccga aattattgaa aaatattata atcaatatcc
     5161 aagcgaaata gaagatggct ttgttgcttt aaatacaatg tttgctcaag atggcgtttt
     5221 tatttatgtg ccagataata tagaagtaaa taaaactatt caattagtaa atcttacaca
     5281 tggttttgga aataaaaata tttttaaacg caaccttttt gttcttggaa aaaattctaa
     5341 gctttctctt gtaatttgtg atcatactct aaacatgagt aataattttg ttgttgatat
     5401 tacagaatct tatgtaggag aaaattctca attagattac cacactatac aaaacgaacc
     5461 aaataaatcg ggagttataa attctcattt tattaattta gaaaaatatt ctcgttgcaa
     5521 ttcttttgta atttctctgc atggtggaat tttgagaaac aatgtgtttg caaaacttgc
     5581 aggcgaaaat tcagaagcta atctttatgg tttaaatctt accgacaaag accaacgtgt
     5641 tgataatttt acgctaatag accatgttgt gccaaattgc atgagcaatg aactttataa
     5701 aggaatttta gacgaacatg cacaaggctc tttcttggga agaatacttg taagaaaaaa
     5761 tgctcaaaaa acagaagctt atcaaacaaa taataacttg tgccttactc ctcaaactaa
     5821 aatgagaaca agaccacagt tggaaattta tgcagatgat gtaaaatgtt cgcatggagc
     5881 tacagtcgga cagcttggac aagatgcact aatgtatttg cgacaaagag gaattgatgc
     5941 aagagaagca aaacttatgc tgatgtttgc ttttgctaat gatgtcttgc taaaagtaaa
     6001 tattccacct cttagagaaa gaattgcaaa tttacttgac tcaagacttc gtggagaatt
     6061 ttcattctgc gaccattgtt tacttaattg taaatcaaga agttagaagt ctggcttttc
     6121 aaaaccataa ataaacaaat tttggttgaa aacatttttt agtttttttt gtcttttatt
     6181 tagattaaat taaatttgca aaatggtcgc tcaacaaaat atagagttaa acaaagaata
     6241 tactgtaagt aaaattacag gtaggtgggg catgaggcgg cgtattagca agcttggaat
     6301 tagtcctaat actgaattaa tttttactaa agaaagtaaa tcaggcttct ataaatttaa
     6361 ttttgttggt tgtaagcgaa aaaaacgact tccagcgaat tatttgagat ttataaaatt
     6421 agttgaatca gataatcctc aacttcaaga aattttagct gaagatcatg ctgaaatagt
     6481 gatagatgta ttttctaatg aagaaacctc tgatacaaat atttttgaaa atcaaacaga
     6541 atataaattt aaaggatata atttttctat caactatttg cctgaaattt caactattta
     6601 tggtgatgat tatccgcgta aaagacttag aaataaagtg ttaacagatt ttcctgatgt
     6661 ttttttaggg atagttgatt ttgaaaactt agaagcagat ttgaatttag taactcagtt
     6721 tatagatatg gatgctaaaa tagttttggc attgcgtaat tataatcctg agtctggctc
     6781 ttataaagct gattttgatt tattaagtaa acttcttggc ttacctatta ttaattactc
     6841 tgaattagag gaagaaaatt cagcacgaga aaagatttta gaaactctaa tcaaaactca
     6901 tcaaaataca gagccttatg tacgacatat ttacataaac tatggaaggc aagttgagca
     6961 atctatttct aaattaaaaa aacttttaaa gcgcagttct aaggctgatt atgtggcatc
     7021 gcctcgttat ttagcaatag gtttattaga aaaagataat ttaatatata gattattcga
     7081 tggtttgcct tgccaaaata aaattaggcg aaaagctaat tatgaaataa aaagattaga
     7141 aaatcttcac aataatcatg tttttaatat agttaaaaac gctcgtaaag cctatgtaaa
     7201 tggtgcaata acagaaatta catctggcaa aaaaactgat acaagatctg aaaaacttga
     7261 taaaatttta actcataaat tttggggaat tccaatcttt ttagctttta tgggattaac
     7321 attttttgct actttcgagc ttggaaaata tccaatggaa tggttggaaa atggaataga
     7381 ttctttatcg aaatatttgg ctaatgtaat gaaacccggc ttctttaagg atttggtgct
     7441 tgatggggca ataggtggag taggaggcgt gttagtgttt ttaccaaata ttttcattct
     7501 atttttcttt ataggtattt tggaaaatac aggatatatg tcgcgggcag cctttttgat
     7561 ggataaatat atgcacaaaa taggcttgca tggaaaatca tttattccaa tgattatggg
     7621 atttggttgt actgtgccag caattatgag tactaaaatc ttagaaaata gacgggatag
     7681 aattttgaca atgatgattt tgccttttat gtcgtgttcg gctaagctac ctgtttatat
     7741 tctaatgatt tctgcatttt tccctgaaca tcctgtttta attttatttg gaatttattt
     7801 gtttggaata attttagctc ttgttgtttc taaatttttt agtaaaacaa ttttaaaaca
     7861 aaaagaagct ccttttgtta tggagctttt gccatatcgt aaacctacag caaaattact
     7921 tttagtttac acatgggata gaggcaaaga atatttgaaa aaaattgcag gagtaatatt
     7981 aataggttct attattattt ggcttttggg atattttcca aaaaatattg attattcaaa
     8041 agattatgaa tcattaattg caaaagaaca gaaacaagaa caaccaaatg cggaaaaaat
     8101 tcatgagtta gagttccaaa aacgtagcga agaacacgaa aaatcttata taggaaaaat
     8161 aggtcatttt gtagcacctg ttatggaacc acttggcttt gattggaaaa tgagcgtttc
     8221 tattcttgct ggcattgcag gtaaagaaat tacagtaagc acaatgggag tcctctatca
     8281 agctgatgaa gacttagaat caggctcttt gcaaaataaa cttaaagccg agacttatgc
     8341 atttggtgaa aaagcaggcg aaaaagttta taataaagct gtagctttag catttatatt
     8401 gtttatttta atttattttc cttgtatagc aacaatctca gctatttgga aagaatcaaa
     8461 acttaaatgg gcaatttttt caggaattta cacaactgca attgcttggg ttgttggttt
     8521 tgcagcttat cagattgcaa aatattttat ataaatttaa aaatataaaa atgaaaaaac
     8581 taatatttta tgtttttttg ggaatttcga ttaatgcgtt ttctcaaaat tcaaatttaa
     8641 catatttaca aggaaatatt attgcaaatc aagattcaat cgaaattata atttctattg
     8701 atgaaaataa ttcagcctat ttgtttgttc ctgagcaatt tctttacaaa atggaatcta
     8761 acgatacttt ttatggtgga gatagtttgt tttgtaattt taagaaaata cctgcgagtt
     8821 tttgtggaaa attaattgat gaaacacaaa ctttttcagg agtttggaag caaggaaaat
     8881 cagaattaaa aactgagttg aaaattactg ataattctga atttgcattt ttaaatcgtc
     8941 ctcaaacacc agaaccacca ttcccttatt tagaaaaaaa tatttgtgtt gaaaatttaa
     9001 aaggaaattc tgttttatgc ggaagtttaa ctattccaga cactttaaaa tcatatcctt
     9061 tggtaatttt agtaagcggt tcgggagctc aagatagaaa cgaagaaatt gcaggacata
     9121 aaacttttat ggtaattgca gactacttaa cacaaaatgg aatagctgtg tttagatatg
     9181 atgatagagg catagctgaa tcaaagggcg attttgcaaa ttctacaact tatgatttta
     9241 tgacagatac tgaggctgta ttaaattatt tttatgattt cccaaatatc gacaaaacaa
     9301 atattggagt tttaggacat agcgaaggtg gactgattgc ttttatgtta gcagcaaaag
     9361 aaacaaaaaa gttaaatttt ataattagtc ttgcaggtcc tggagtgcca attaaagagc
     9421 ttttggttca gcaattgaaa gatgtaaata aaagttttaa ttttcctgat gagcatattg
     9481 aagttatgtg cgaaatgcaa tccgaaattc ttgatatagc aacaaaaaat gtggatatgt
     9541 atgagttgcg aaaaaatatt aaaattattt acgataaata tgctcagcat tttaccgaag
     9601 aagaaagaca aaaatatcaa attggagatg ctgcaataaa tgttgctgtt atgcaattat
     9661 catcgccttg gatgaaatat tttataaaaa ttgacccagg caaatatatt aagaaagtaa
     9721 aatgcagggt tttatctata aatggttcta ctgacttgca agtaaaaatg gaagataact
     9781 taaatagtat tgaaaagaat ttaaaaacta aaaaagaagt acaaattgtt gctattgaag
     9841 gtcttaacca tttattccaa aaatcagaaa ctggtgcaat tagtgaatat tatacaattc
     9901 aaaacactat agaacaactt gttttagata aaattgttga gtttataaag taaaaagttt
     9961 ttaaagatca taaaggcgtt aatttttatt actatctccg ctcgctcgga tttgtaatcc
    10021 gagtgtaaca ttaccaagca ctcggattgc aaatccgagc gagcgtcatg gttgttggag
    10081 cgagctaatc gtagcttaat tcgagcatgc gaagaagtgt gtacgattca ataaactcgt
    10141 ttaaaagttt ttaagcttta aaaatttaaa aaaaaatagt aatagattga tttttccatt
    10201 aaaaataatt atatttgttt tattaattca ttgacagatt aataccaagt tgcgttctat
    10261 tataaaacat taagataagg aaaaagtgtc at
//
LOCUS       DAOPNX010000091        10213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_7472_length_10213_cov_6.702993, whole genome shotgun sequence.
ACCESSION   DAOPNX010000091 DAOPNX010000000
VERSION     DAOPNX010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10213
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7472_length_10213_cov_6.702993"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..1926)
                     /locus_tag="PKN53_09185"
     CDS             complement(214..1926)
                     /locus_tag="PKN53_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD89162.1"
                     /translation="MKLDYRQRLFLYFGLLFTLFTVGIIIFEQFREKNYKTQGLKEKL
                     ETYTDIIQVAIEHSHDSVAPVINQLQNLLPQDLRITIISEEGKVLFDNSINDYISLDN
                     HKDRPEILEALQSGKGTDIRVSESNKQKYLYFAKKADDKFIRVALLYNIKLQNFLKPD
                     NAFLYFIILFFAVFIFFIHVTTKRLGKTIKQLRDYVLNSDKHDLDKLNFPNNEIGEIG
                     SKIADNYFKLKESQKTVLMERQKLLQHIQVLEEGICFLSPKHEVEYHNGLFIQYLNII
                     ADEASSDASIILTDENFSEIQDFLSQDEESYFEKIISKQGKIFAVRANLFKDGSFEVI
                     LSDITKEEKTRRLKQEMTGNIAHELRTPITSIRGYLETVLDQPLSDEKKQYFIERAFH
                     QTIALSEIIQDMSLITKMEEAPNLFNLEKTNIVQLLERVKEDLSIRLVEKHIEMKWQV
                     PEQLEITGNENLLYSLFRNLTDNAIRYGGENMCINVSIYNEDADFYYFSFYDTGIGVK
                     DETYLNRIFERFFRISEGRTRDNGGTGLGLSIVKNAVLFHKGNISVKNRKEGGLEFLF
                     TLKK"
     gene            complement(1986..2674)
                     /locus_tag="PKN53_09190"
                     /pseudo
     CDS             complement(1986..2674)
                     /locus_tag="PKN53_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841561.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(2802..4073)
                     /locus_tag="PKN53_09195"
     CDS             complement(2802..4073)
                     /locus_tag="PKN53_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89163.1"
                     /translation="MIKLKQLAIKLLFIWGLGLLGITQSYSQVVDLETLMQLDEDTSK
                     LEILTGLKVSGYFQGQFQYGQKDADLGVGAPNTDVTENFNRIGIRRGRLKFTYEKRIA
                     LAVLQFDMNETNIRVKDAYMQFNDLKYSQSSLKVGAFYRPFGYEVSHSSSLRESPERA
                     HVITTLFPEERDLGVMLTLQAKKSSAWSIFKIDAGLFAGNGVKADIKNKKDFIGRFSI
                     NKSFKDYLGISGGVSYYNGHVYQGTTDVYSMYDKIFIPNSSMANIGKYAKREYIGVNA
                     KIVAITKLGMSSITGEFIAGVQPGRKDGSKSPNSSTVGSTDTYIRDFYGAYACLVQDF
                     GKLPLSAVVKYEWYDPNNKVSKDEIGLFGTGIGDVSYNTLGFGLIWKVNPDIRATVYY
                     EMAKNEISQNLSNFENDIENNIFTLRLQYKF"
     gene            complement(4207..4680)
                     /locus_tag="PKN53_09200"
     CDS             complement(4207..4680)
                     /locus_tag="PKN53_09200"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOD89164.1"
                     /translation="MKKIKKIALVAHDNCKQELIEWVEFNWRLLIQNDLTCTGTTGKL
                     IEKTLKKCIEQNSEEKKIDITKLKSGPLGGDQQLGALICNGEIDVLIFFWDALQPQPH
                     DVDIKALLRLATLYNIVYACSRSTADFVISSPLFKENYKPTINEQIDDYKNRKLG"
     gene            complement(4686..5495)
                     /locus_tag="PKN53_09205"
     CDS             complement(4686..5495)
                     /locus_tag="PKN53_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOD89165.1"
                     /translation="MKDKVVIITGASSGIGKALAIEFAKKGSKVVLAARNPENLNSVL
                     DEVKQYNTEAIAVVADVSLVDDCENLIKKTIETFGKINILINNAGISMRSMFLDTDLL
                     VMHKLMDTNYWGTVNCTKFALPYLVEQKGSLVGIISIAGYLGLPARSAYSSSKYAIRG
                     FLDTIRVEHLKSGLHVLVAAPGFTASNIRKVALLGDGSLQGATPRQEEKMMTAEQVAK
                     KIYRAIVKRKRTLILTFVEGKLAVFLSKWIPGLLDKLNYNHMAKEENSPLK"
     gene            5817..6533
                     /gene="dapB"
                     /locus_tag="PKN53_09210"
     CDS             5817..6533
                     /gene="dapB"
                     /locus_tag="PKN53_09210"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOD89166.1"
                     /translation="MKIVLIGYGKMGKTIESIAIQRGHVITEIIDIDNKNKINQDLKN
                     KCDVAIEFSNPESSKTNILNCLNNGISVVSGTTGWKLDESEFIELTKKTSTAFFYASN
                     YSIGMNVFMEINKRLATLLNNYSDYEAKIEETHHIHKLDKPSGTAISLAKQILENNSK
                     YSNWELSPTQNKQSLEVEAFRIDEVFGDHKVIWKNNIDTIEISHSASSRLGFATGAIL
                     AAEYIFGKTGYFTMKNLLNF"
     gene            6536..8155
                     /gene="lepB"
                     /locus_tag="PKN53_09215"
     CDS             6536..8155
                     /gene="lepB"
                     /locus_tag="PKN53_09215"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOD89167.1"
                     /translation="MAKKTGFKSKIKNNPIIQKLKNKWIWFSVTTLLYVLWVIWLGNY
                     WWLLGELIIIDIYITKFVRWAFWKPRKDKTYSKAKRKTLEWIDAIIFAVIAASFIRIF
                     FFEAFTIPTSSMEKTLLVGDYLFVSKVAYGPRVPMTPISFPFVHHTLPLTKSTKSYVT
                     WIQTEYRRMAGWGDVKRDDMVVFNYPTGDTVVVQNQAQDYYDIIRICSFIMEQFDLEI
                     LEATKDTNLMMGVKDTHYYNSLAYNLFDPNINEYHNTEMINNASLKLSELDKEFYKSG
                     NRLKTQKDYDKLARELVREVYDIIVRPIDKRENYIKRCVAIAGDTLEIKEGEVYINGT
                     KQKNIPTKQFNYIVNIENGNISERFLRENQINRSDIRRFNNSSLIIPLNELVLQKVQK
                     LAYVKSVKPDFRPKGERDFRIYPHNENFDWNADWFGPLYIPKAGATIELNEKNLILYE
                     RLIGYYEDNTLEIKDGKAFINGKQTNTYTFRMNYYWLMGDNRHNSADSRFWGFVPENH
                     VVGKAWLIWMSSDKEYGGIRWSRIGKIVHNE"
     gene            8148..9026
                     /locus_tag="PKN53_09220"
     CDS             8148..9026
                     /locus_tag="PKN53_09220"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOD89168.1"
                     /translation="MNKKLKRKLIIISSIVLLLIILFFTYTGFYKAKSCEGKKQTTFI
                     LKVKNPNMNLNFLKPSADFLLDYSDKIVYKSPELSDPPFSKKENIIGRVIGKPGDIVE
                     IRDTKILINNKELNEDYDLMFKFRVNTVEETDFAELLKNHDVKILDTLLNNKACDILT
                     YKHIADEISKLEGVMVVRRITEPEAVLNYNTFPFQIFPWNQDFFGPTVVPCKDMTLSL
                     NRRNIHLYKKIIDFYEGHALYYFGENIEIDENLVDKYIVEKNYYFVLSDNRLNGKDSR
                     RWGFIPEEYIIGKIAF"
     gene            9026..9640
                     /locus_tag="PKN53_09225"
     CDS             9026..9640
                     /locus_tag="PKN53_09225"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOD89169.1"
                     /translation="MEAVLFSSYFPSIQYISKFFIYSNIYIDIFENYQRQTYRNRCEI
                     MTANGKANLIVPVIKSYRQYVKDTKIDYSTDWQKNHYMALVSAYKNSAFFDFYIDSFN
                     FVFKNKEKFLFDLNHKILENCFKILGIKPKFEYSKDFINQKDIINLRELISPKSKLVD
                     KNFALKEYYQVFNEKHGFTPNLSIIDLLFNEGNLASLIIKEAVK"
     gene            complement(9688..>10213)
                     /locus_tag="PKN53_09230"
     CDS             complement(9688..>10213)
                     /locus_tag="PKN53_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89170.1"
                     /translation="TLKVKTKSAVFYEMTREYAIIDGKKELVYIEPFSYYYYYHISTT
                     IKNNTKGNVFIDLNTCKLKIGDKCLSMRTCVNGINTANKIDTLIIKPKEKILFNLYDE
                     NGDFFFSDLDFSLYIDFYDNFSKYLKKLNENTILEFECSGEINAKISIPITTFITYNE
                     ESEISKFRNKHRCE"
BASE COUNT         3528 a         1587 c         1442 g         3656 t
ORIGIN      
        1 cgctgattaa caacggatta gctttggtgt cccaaaaaag tgcggaactc aggaaaaata
       61 tttttttatc tgccacaaaa acaatttttt agttttattt tcgctgcaaa attggtgtct
      121 gaatattaca aagttgttac gcctaagtta attggcagtt aaaagttgaa agttaaaaac
      181 taaaagtaga tgattgattt tcaggcagtt ctatcacttt ttcaaagtaa ataaaaattc
      241 taaaccgcct tcttttctat ttttcacgct tatatttcct ttatgaaata agactgcatt
      301 ttttactata gacagaccta atcctgtacc gccgttgtcg cgtgttcgtc cttcgcttat
      361 gcggaaaaaa cgttcaaaaa tacgatttaa ataggtttcg tcttttacac caattccagt
      421 atcgtaaaat gaaaaataat aaaagtcagc atcttcgttg taaatgctca cattaatgca
      481 catattttct ccgccatatc tgattgcatt atctgtaaga tttctaaata aggaataaag
      541 caaattttca tttcctgtaa tttctaattg ctctggcact tgccatttca tctcaatatg
      601 cttttcaact agtctaatgc taaggtcttc cttaactctt tcgagcagtt gtacaatatt
      661 tgttttttct aaattaaaca aatttggagc ttcctccatt tttgtaatta aactcatatc
      721 ctgtataatt tctgaaagag caatagtttg gtgaaaagca cgctctataa aatactgttt
      781 cttttcatca ctcaatggct ggtctaagac agtttctaaa tatcctctaa tgcttgtaat
      841 tggtgtacga agttcgtgag caatatttcc tgtcatttct tgtttcaaac gtcttgtttt
      901 ttcttctttt gtaatatcac tcaaaataac ttcaaaactt ccatctttga aaagattggc
      961 tcttactgca aatattttac cttgtttgct aattattttt tcaaaataag attcctcatc
     1021 ttgagataaa aaatcttgta tctcactaaa gttttcgtct gttaaaatta tagaagcatc
     1081 gctagaagcc tcatctgcta ttatattaag atattgaata aacaagccat tgtgatattc
     1141 cacttcatgc ttaggcgaca aaaagcaaat tccttcttct aaaacttgaa tatgttgcaa
     1201 aagtttttgt ctttccatta aaacagtttt ttggctctct tttagcttga aataattatc
     1261 tgctatcttg cttccaattt cgccgatttc attattagga aagtttaatt tatcaagatc
     1321 atgtttgtca gaatttaaaa cataatctcg caattgcttg atagtttttc caagcctttt
     1381 agtagttaca tgaataaaga aaataaaaac tgcaaagaac aaaataataa aatacagaaa
     1441 agcattatcg ggttttaaaa agttttgaag tttaatatta taaagtaaag ctacgcgtat
     1501 aaacttgtcg tctgcttttt ttgcaaaata taaatacttt tgcttgttag attctgacac
     1561 gcgtatatct gtgccttttc ctgattgcag tgcttctaga atttctggtc tatctttatg
     1621 attatcaagg cttatgtagt cgttgatgga gttgtcaaac agcacttttc cttcttcgct
     1681 gataattgtt attctaaggt cttgtggcaa tagattttgc aactgattaa ttactggagc
     1741 cacactgtcg tgagaatgtt ctatagcaac ttgaataata tctgtatagg tttctaattt
     1801 ttcttttaga ccttgagttt tgtagttttt ttctctaaat tgttcaaata taataatacc
     1861 aaccgtaaag agtgtaaaca acaaaccaaa atatagaaat agcctttgtc gataatctaa
     1921 tttcatttat tcaaaatttt cacgccaatt ataattagtt aattttcaat catttaggct
     1981 aatgcttata attcatttac atcaaatctg tatccgtatc ctattttatt tgaaattact
     2041 gatgcgtaat ttcctagttt ttttctcatt cgtgttatat gcacatcaac agttctttct
     2101 gttacataag gtgtatctgt ccataattta cttattatcc tttctctaga taataattcg
     2161 tctggatgtt gggcaagtag agacaataat tcaaattctg tttttgtcaa tggaattttt
     2221 ttgtcatcaa tgctgagttc tttcatatta aagtctatga caaaatttcc ataaaccaac
     2281 ttattctcac tactatctgt tttatcttgt ttttctgttc gttttaaaac tgctcttact
     2341 cttgcaataa cttctttgat agaaaatggt tttgcaatat aatcgtctcc acctacagaa
     2401 aaacctgtaa gcatgtcatt ttcagtgtct tttgctgtta aaaatataac tggtgttgaa
     2461 atgccatctt ctcgcatttt ttctgccatt ttaaatccag acattccacc cacatcacat
     2521 ctagtagtat taaatgaaat tttttaagat ttttacttaa agcttcttct gccgaaacag
     2581 cagtttctac atcatagcct tcacttttga gattaaactc taaaatttca gcaatgctaa
     2641 gatcatcatc aactattaaa atatttttac tcataaaatt ttgtttcaca ctacaaatgt
     2701 agttatttta aatcaatata ataattattt tcctagttta aacaaaagta tattcagtat
     2761 gttacataag actttattta ttgttacaat cctgttacag tttagaattt atattgaagt
     2821 ctaagggtaa aaatattatt ttcaatatcg ttttcgaaat tgctaagatt ttgcgatatt
     2881 tcatttttag ccatttcata atagacagta gctcttatat ctggattaac tttccatatt
     2941 aaaccaaaac ctaaagtgtt gtaagaaaca tctcctatgc cagttccaaa caagcctatt
     3001 tcgtcttttg agactttatt atttgggtca taccattcat atttcactac tgctgacaag
     3061 ggtaattttc caaaatcttg aactaaacaa gcataagcac cataaaaatc tcggatataa
     3121 gtatctgtac tgccgacagt tgaactattt ggacttttac tgccatcttt tcttccaggc
     3181 tgtactcctg caataaattc tccagtaata gaagacattc ccaacttggt gattgctaca
     3241 attttagcat ttacacctat atattctctt tttgcatatt ttccaatatt agccatagaa
     3301 ctattaggta taaatatttt atcatacata gaataaacat ctgttgtgcc ttgatatacg
     3361 tgtccattat agtaagaaac accgccactt attcctaaat aatctttaaa gcttttattt
     3421 atagaaaaac gtcctataaa gtctttttta tttttaatat ctgctttaac gccattgcct
     3481 gcaaacaagc cagcatctat tttaaaaata ctccatgccg agcttttctt tgcttgtaaa
     3541 gttagcatta ctcccaaatc tctttcttcg gggaataaag tagtaataac atgtgctcgt
     3601 tctggcgatt ctcttaacga agaagaatgt gatacctcat aaccaaaagg gcgataaaaa
     3661 gctcccactt taagagaact ttgagaatat tttaaatcat taaattgcat atacgcatct
     3721 tttactctta tgttagtttc attcatatca aattgtaaaa ctgctaaagc aatgcgtttt
     3781 tcgtatgtaa attttaagcg acctctgcga ataccgatac gattaaaatt ttctgttaca
     3841 tcagtattag gtgctcctac tcccaaatct gcatcttttt gtccgtactg aaactgccct
     3901 tgaaaataac ccgagacttt tagcccagtt aaaatttcaa gtttgcttgt gtcttcgtct
     3961 aattgcatca gagtctctaa atccacaact tgtgagtaag attgagttat tcctaataaa
     4021 cctaagcccc aaataaatag caatttgatt gctaattgtt ttagtttaat catgtcctat
     4081 ttagtcaagc aataaagcta caaacataag aaaaagatat taatttcttg ctttaaaaca
     4141 taaaaaatta ttaagaattg attgtttaga ataaattgct ctggctaatg ctttaaagct
     4201 tagattttat cccaactttc tgttcttata gtcatcgatt tgttcgttta ttgtaggctt
     4261 gtaattttct ttaaacaatg gtgaagaaat aacaaaatcg gctgttgacc tagagcaagc
     4321 ataaactata ttatataaag ttgctaatcg cagtaatgcc ttaatgtcca catcgtgtgg
     4381 ttgtggttgt aaagcgtccc aaaagaaaat tagcacatct atttctccat tacaaattaa
     4441 agcacccaac tgctgatcgc cacctaaagg tcctgatttt aattttgtaa tgtctatttt
     4501 cttttcttct gagttttgct ctatacattt ttttaaggtt ttctctatca acttccctgt
     4561 tgttcctgtg caggttaaat cgttttgaat aagaagccgc caattaaact ctacccattc
     4621 tatgagttct tgtttgcaat tatcgtgtgc tacgagtgct atttttttaa tttttttcat
     4681 cttttttact ttaatggtga attttcttcc ttagccatgt gattataatt taacttatcc
     4741 aacaaaccag gtatccattt tgataaaaac actgctaatt taccttccac aaaagttaat
     4801 attaaagtcc gttttcgttt tacaatagct ctatatattt tttttgctac ctgttcggca
     4861 gtcatcattt tttcttcttg tcgtggtgtc gctccttgta aacttccgtc tccgagaagt
     4921 gccacttttc ttatgttaga agctgtaaag ccaggtgctg caactaaaac atgcaatcca
     4981 gattttaaat gctcaactct aatagtatct aaaaagcctc tgatagcata ttttgatgac
     5041 gaataagccg agcgtgcggg caagcctaaa tatccagcta ttgatattat accaactaaa
     5101 cttccttttt gttcgaccaa atatggcaaa gcaaattttg tgcaattaac tgttccccaa
     5161 tagtttgtgt ccataagttt gtgcataacc aacaagtcag tatctaaaaa cattgaacgc
     5221 atactaatac ctgcattgtt tattaaaata tttattttgc caaaagtttc tattgttttt
     5281 tttattaaat tctcacaatc atcaacaaga cttacatctg ccactacagc gattgcctca
     5341 gtattgtatt gtttaacttc gtctaaaact gagtttaaat tttcaggatt gcgagcagct
     5401 aaaacaactt tacttccttt ttttgcaaat tctattgcca aagctttgcc aattccagac
     5461 gatgctcctg ttattattac aaccttatct ttcatttttt ttgttttttg caaagttaaa
     5521 aaaatcagtt aaaagttaaa agagttttta gtgttgagtt tttagttttt agtattaaaa
     5581 gaatgaagaa ttaagtttat gagcttttca ctttttaaac ttttaattaa gatgattatc
     5641 aaagagttaa tgtatattac aaaaaagtgc aaaaaacaaa agattaagat taaaatttta
     5701 aaaaacagtt tgcatcttaa aggtttttaa gtttttatta aatattaata ttatttgatt
     5761 ttatgaaaac ataaaatttg ttgtatcttt acaacgcttt aaaaattaat attcttatga
     5821 aaattgtttt aataggttat ggtaaaatgg gtaaaactat tgaaagtata gctatccaac
     5881 gtggacatgt aattaccgaa attattgata ttgacaataa aaacaaaata aaccaagact
     5941 taaaaaacaa atgtgatgtc gcaatagagt ttagtaatcc tgaatcttca aaaacaaata
     6001 ttttaaactg ccttaataat gggatttctg ttgttagcgg aactactggc tggaaacttg
     6061 acgaatcaga atttatagaa cttaccaaaa aaacatcaac agcttttttc tacgcttcaa
     6121 attatagtat tggaatgaat gtttttatgg aaataaacaa aagacttgct actttattaa
     6181 ataattattc cgactacgaa gcaaaaattg aagaaacaca tcacatacac aaactcgaca
     6241 aacctagtgg tacagcaatt agcttagcaa aacaaatttt agaaaacaat tctaaatatt
     6301 caaattggga attatcgcca acacaaaaca agcaaagtct tgaagttgag gcttttagaa
     6361 tagatgaagt ttttggcgac cataaagtta tttggaaaaa caatattgat acaatagaaa
     6421 taagccattc tgcctcaagt cgtttaggat ttgcaacagg agcaatactt gcagcagaat
     6481 atatctttgg caaaacaggt tactttacaa tgaaaaattt attaaatttt taactatggc
     6541 aaaaaaaaca gggtttaaat ctaaaataaa aaacaaccca attatacaaa aattaaaaaa
     6601 taaatggata tggttttcgg taaccacttt gctttatgtg ttgtgggtaa tttggttagg
     6661 aaactattgg tggttgcttg gcgaacttat tattatagat atttatataa caaaatttgt
     6721 tagatgggct ttttggaaac caagaaaaga taaaacttat tcaaaagcta aaagaaaaac
     6781 tttagaatgg attgatgcaa ttatatttgc agtaattgcg gcttcattta ttcgcatttt
     6841 ctttttcgaa gcttttacaa taccaacttc ctcaatggaa aaaacccttt tagttggtga
     6901 ttatttgttt gtaagtaaag tagcctacgg acccagagta ccaatgacac caatttcttt
     6961 tccttttgtt catcacactt tgccattaac taaaagcact aaatcttacg taacttggat
     7021 tcaaactgaa tatagacgaa tggctggctg gggcgatgtc aaaagagatg acatggtagt
     7081 ctttaactat ccgacaggag atacggttgt ggtacaaaat caagctcaag attattacga
     7141 cattataaga atttgttctt ttattatgga acaatttgac ttagaaattt tagaagctac
     7201 aaaagacaca aatcttatga tgggagttaa agacacacat tattataatt ctcttgctta
     7261 taatttattt gacccaaaca taaacgaata tcataatact gaaatgataa acaatgcaag
     7321 tttaaagttg agcgaattag acaaagaatt ttacaaatct ggcaacagac taaaaacaca
     7381 aaaagattat gacaaacttg caagagaatt agttcgtgaa gtttatgaca ttattgttcg
     7441 cccaatagat aaaagagaaa attatattaa aagatgtgtt gctattgctg gtgacacttt
     7501 agaaataaaa gaaggcgaag tatatattaa tggtacaaaa caaaaaaaca ttcctacaaa
     7561 acaatttaat tatatcgtta atattgaaaa tggaaatatt tctgaaagat ttttaagaga
     7621 aaatcaaata aatagaagtg atattagacg atttaataat tcatctttaa taattccatt
     7681 aaacgagctt gttttgcaga aagttcaaaa attagcttat gtaaaaagtg ttaagccaga
     7741 ttttagacca aaaggcgaaa gagattttag aatttatcct cataatgaaa actttgattg
     7801 gaacgccgat tggtttggtc ctttgtatat tccaaaagct ggggctacta tagagcttaa
     7861 cgaaaaaaat ctaattttat atgaaaggct aataggctat tacgaagaca atactttaga
     7921 aataaaagac ggcaaagcat ttataaatgg aaaacaaacc aacacatata ctttcaggat
     7981 gaattattat tggcttatgg gtgataatag acacaactcg gcagattcga gattttgggg
     8041 atttgtacca gaaaatcacg ttgttggtaa agcatggcta atatggatgt cgtctgataa
     8101 agaatatggt ggaataagat ggtcaagaat tggaaaaatt gtacacaatg aataaaaaat
     8161 tgaaaagaaa acttataatt ataagttcta tagttttatt gcttattatt ttatttttca
     8221 cttacacagg tttttacaaa gctaaatctt gtgaaggtaa gaaacaaact acgtttatat
     8281 taaaagtaaa aaatccaaat atgaatttga actttttaaa accatcagcg gattttttat
     8341 tggattatag cgataaaatt gtttataaat caccagaact aagcgaccct cctttttcaa
     8401 aaaaagaaaa tataatagga agagtaattg gaaaacctgg agatattgtt gaaattagag
     8461 atacaaaaat attgataaac aataaagaat taaacgaaga ttatgatttg atgtttaagt
     8521 ttagagttaa cacagtagaa gaaacagact tcgcggaact tcttaaaaat cacgacgtta
     8581 aaatattaga cacattactt aataacaagg cttgcgatat tttaacttat aaacatattg
     8641 ctgatgaaat ttcaaaactc gaaggtgtaa tggtagttag aagaattaca gaacctgaag
     8701 cagttttgaa ttataacact tttccatttc aaattttccc atggaatcaa gatttttttg
     8761 ggcctacagt agtgccttgc aaggacatga ctttaagtct aaacagacgc aatatacatt
     8821 tgtacaaaaa aataattgac ttttacgaag gacatgctct ttactatttt ggcgaaaaca
     8881 tcgaaattga cgaaaattta gtagataaat atatagtcga aaaaaattat tactttgttt
     8941 taagtgataa tagattaaat ggaaaagatt ccagaagatg gggatttatt ccagaagaat
     9001 atattattgg caagattgca ttttaatgga agcagtatta ttttcttctt actttccttc
     9061 tattcagtat atttctaaat tttttattta cagcaatatt tatattgata tttttgaaaa
     9121 ctaccaaagg caaacttaca gaaaccgttg cgaaattatg actgccaacg gaaaagccaa
     9181 cttaattgtg ccagtaatta aatcttacag acaatatgta aaagatacta aaatagatta
     9241 ttctacagat tggcaaaaaa atcattatat ggctttagtt tctgcttata aaaactcagc
     9301 tttttttgat ttttatatcg acagttttaa ttttgttttt aaaaataaag aaaaattttt
     9361 gtttgacctt aatcataaaa ttcttgaaaa ttgctttaaa attttaggta taaaaccaaa
     9421 atttgagtac agtaaggact ttatcaatca aaaagatata ataaatttaa gagaacttat
     9481 ttcgccaaaa tcaaaacttg ttgacaaaaa ttttgcactt aaagaatatt accaagtctt
     9541 taacgaaaag catggattta caccaaattt aagcataatt gatttattat ttaacgaagg
     9601 aaacctagca agtttgataa taaaggaagc ggtgaaataa aaaataaaaa ctctgctaat
     9661 gcacttttta atagcttcta atctacttta ctcacatcta tgtttattgc gaaattttga
     9721 aatctcagat tcttcattgt atgttataaa agttgttatt gggatactaa ttttggcatt
     9781 tatttcacct gaacattcaa actcaagtat agtattttca tttaattttt tcaaatactt
     9841 actaaaatta tcatagaaat ctatatataa agaaaaatct aggtcgctaa agaaaaaatc
     9901 tccattttca tcatacaaat taaataagat tttttctttt ggtttaatta tcaatgtgtc
     9961 aattttattg gctgtattta ttccatttac acaggtcctc attgagagac atttatctcc
    10021 aatctttaat ttacatgtgt ttaaatcaat aaaaacatta ccttttgtat tatttttaat
    10081 ggtggtgcta atatgataat aatagtaata actaaaaggt tcgatataaa ctaattcttt
    10141 ttttccatca attattgcat attctctagt catttcataa aaaacagcag atttagtctt
    10201 aactttaaga gtt
//
LOCUS       DAOPNX010000092         9958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_7712_length_9958_cov_6.099061, whole genome shotgun sequence.
ACCESSION   DAOPNX010000092 DAOPNX010000000
VERSION     DAOPNX010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9958
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7712_length_9958_cov_6.099061"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..517)
                     /locus_tag="PKN53_09235"
     CDS             complement(47..517)
                     /locus_tag="PKN53_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89171.1"
                     /translation="MEKKITPITICIDFDGTCVTHEFPKVGKDIGAVPVLKKLIESGH
                     QLVLFTMRSDIVNPTGEDNELHLESGNYLTDAVNWFKENEIPLYGIQTNPTQHTWTTS
                     PKAYAQLYIDDAALGCPLLYGNFGENGEGFYFDKPQVDWEKVKDILIKNGIINV"
     gene            complement(502..1203)
                     /locus_tag="PKN53_09240"
     CDS             complement(502..1203)
                     /locus_tag="PKN53_09240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOD89172.1"
                     /translation="MKVIYSIQCKITNKQYIGSALNHEKRKREHFYNLKNNKHPNKKL
                     QNAYNKYGADCFEFKIIEVVNDEKKLIEREQHYIDNLKPIYNICRVAGSSLGLKRTND
                     TRDKIRAANLGIKHPQWRNEIKSKAQGGENHWTKRKGFSNKSKKQMSATHKEKYRSGY
                     RNPLKGTRYTAERIEAMRIMSSQPIIQYDLNLNPVKEWNSAKEAKQCGFIPSNISACI
                     NGLRKTHKKFIWKRK"
     gene            1387..2529
                     /locus_tag="PKN53_09245"
     CDS             1387..2529
                     /locus_tag="PKN53_09245"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HOD89173.1"
                     /translation="MFRAYKYRLYPSELQKELIAKHIGSSRFVYNLALETKTTAYLGS
                     KHNFSPFDLIKQLPELKKECEWLKEVNSQSLQQSIQNMDIAFKKFFKGAGFPKFKSKR
                     DRQSFSIPQNVIVENNLLIIPKFKEGIDIVLHRPIKGTIKSATISVTPTGKYFVSILC
                     DTKKDIPIKAPIEENTTIGIDLGIKDFAITSDGEVFENPKYLRKAQSKLKYAQRKYSK
                     HKGKRTKKKLAKLYEDVVNKRKDFLHKTSTKLIRENQTICLETLAVKNMVKNHNLAQA
                     INDVSWSTFVSMLEYKADWYGKNILRIGRFAPSSKTCSNCGNINKELTLKDREWTCGS
                     CSTVLDRDVNAAINIKNFALKNILSGTDRKNQGKLPTLVGALTLEA"
     gene            complement(2593..2748)
                     /locus_tag="PKN53_09250"
     CDS             complement(2593..2748)
                     /locus_tag="PKN53_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89174.1"
                     /translation="MLRRDHATIIYAVKTTAKWYECVGYDYEIGIYEDVNASVTGWIK
                     SFMYGTI"
     assembly_gap    3143..3242
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3530..5260)
                     /gene="dnaG"
                     /locus_tag="PKN53_09255"
     CDS             complement(3530..5260)
                     /gene="dnaG"
                     /locus_tag="PKN53_09255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOD89175.1"
                     /translation="MGKISQNTIKSVSSINIVDVIGSYIHLKKQGGIYRACCPFHNEK
                     TPSFIVNQNNNFYKCFGCGEGGDVISFVMKYEALSFYDTITTLCDKYKIELKEDGGNE
                     LDATQLETNKILEINKVTTEFFTYKLPNSLSHRLIKERGLSDEVISLFQIGHTSSLYS
                     QLYKYLIEKGFTNEEVSKYGLCRIKADNTYYDTFRDRTIFPIHNSIGKVVGFSARRNI
                     ETMQPKYINTSENRVFKKSKELFGLYFAKKHITEKDEAIVVEGATDVISLFDNGVKNT
                     VGTLGTAFTTEHATTLKRYTQNIVILYDGDNAGLKATFNACEELLKAGMNIYICPLPK
                     DKDPDNIARELKNKTNGYIQSNKIEFIKHRISLLKDFNIIDKVKLAKELIRMINLHPD
                     KITRDILLQTYNKEFGFNNTTKPKKHHTVLEIEKETNIELHLIRIILIYLNEWNILNY
                     FNNIDKNFYPDMFENKRCLLFFEYINSEDCESFELSDFIHNKDVAVREISNLALGLDY
                     NIENTEQYDIESTLYSFELKVAERLKNKILLNPTLDYKHKLKVITKHENIIQQNIIKL
                     SENKNQNILA"
     gene            complement(5260..5481)
                     /locus_tag="PKN53_09260"
     CDS             complement(5260..5481)
                     /locus_tag="PKN53_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89176.1"
                     /translation="MKDNNTAEGLNVKTINGKHGSFQVLRVYIPKFSKWIEENKDKND
                     CITLALYPRREADEYNNTHTPKKFTSNNQ"
     gene            complement(5471..5968)
                     /locus_tag="PKN53_09265"
     CDS             complement(5471..5968)
                     /locus_tag="PKN53_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89177.1"
                     /translation="MEFNLENKEQVYIGIDVGGSGAIAITNGREILNVITNDNTDADL
                     SEFIQEAKDRYDIVKCMIENVHSMPKQGVSSSFKFGESKGFLRGLIVAHKIPFEEASP
                     QKWMKHYGLSGKGFDSKTEHKNNLKNYAQRLYPNMKITLKTADAILLAHYSFLINNNL
                     YNNER"
     gene            complement(5974..7164)
                     /locus_tag="PKN53_09270"
     CDS             complement(5974..7164)
                     /locus_tag="PKN53_09270"
                     /inference="COORDINATES: protein motif:HMM:NF021128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HOD89178.1"
                     /translation="MNQTQTTQTLHSKEYYNKERGVYIERIPEKDEEWHKRRSNGIGA
                     SDIGRILGLNDYEGGSCIEIFYEKIGLKPAFRESNKYTFWGHQQEELIANAWKYYDGT
                     KDSYIFNYEKNNKVRDCRNVHGVLSNEKYPHLFTNIDRLITKGCFKLTDGTMLEDNGI
                     LECKTGSSWVAKKWQEGIDPMYVMQVQQQLMIMGLEYAEIAFLELDNRELWVYPVEAN
                     KSLISQIEEQSYVFWYKMVLPAKKLVAERELELNRGNMDAVRKIEYEIQKLEPSADGS
                     ERYKDFMNQRWLSEPDIIKADESQLINALELKYYKELIKELERDVTLKENIIRQFMGD
                     NINISTNKTPDTLDFDEYGKITWKTEGKNLDRKVLRTISFKGDVSDSILKSKEQINNN
                     KPKI"
     gene            complement(7127..8578)
                     /locus_tag="PKN53_09275"
     CDS             complement(7127..8578)
                     /locus_tag="PKN53_09275"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HOD89179.1"
                     /translation="MSDLKLEFSILGAIINDEPNKVIAIQKINKKEFFEYKISQYVFS
                     AIYDLFINQSEVDFFTVKDRLIKNNHYQQIGDDVYSEITSNTLSLPTLELRIEVLKKD
                     YLYRVQVDVAKDILNKAQKGEIPPDELILEAINSLESSLYGENIDTSKPVGILLKDMM
                     LGLLNESEKELGYTTGYKEIDDYFTLLPGTLVTFAARSSAGKTAFLLQVLRRLSFLNN
                     IPCAVFSLEMRASAYVTRITSAHLKINSYAFRNNLLGDKDKDRFHNFVTTFSASPLFI
                     DDNPEYDVHSILAKIIVLAITKHIKVFAIDYLTLVKLHYETKQTTKSDAIGYFTFALK
                     KIARKLNVTIIILQQLNKELDKRPNPRPIIGDLKDSGSIENDSDVVVLFYRPDMYTQN
                     IFEARSKDERDTYGHLEGKYFLLHKNGRNITPIEMSNKCEVIVAKNREGKTGSLFFDF
                     IKGYGGFDYNNELSSILNNKKNESNTNNTDIAF"
     gene            complement(8584..9570)
                     /locus_tag="PKN53_09280"
     CDS             complement(8584..9570)
                     /locus_tag="PKN53_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOD89180.1"
                     /translation="MNLDFKYQEEKTQPKECRIIPLHPLQVKAINLEKHVEEFIAWRQ
                     VAKNTAMNYRSVLNKMLKECGEPLNWNCIQDYINSLHYNYKESHVFRVSNTLKKFFEY
                     LDSHTEFENKVYNRLFIKKYRYEGGNKDVISDKKFLEIYKLFDFRKSADIRDYAILKL
                     LQSTGMRVGEPFRIKISDIKTISDAFGNNYTIIDSTQKGGRRLVCRVHDGTLKAINTY
                     LRTRGFYDKNEPLFIQHKFHFNKNERYKPLNVTVFCKRLKQLFEAVGLEDYSSHSIRY
                     TAAFKTLNATNDETKASAKLGHISPKTIQYYTHNYRLELNAINDTNIELNTK"
     gene            9639..9884
                     /locus_tag="PKN53_09285"
     CDS             9639..9884
                     /locus_tag="PKN53_09285"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOD89181.1"
                     /translation="MKKNKHTDLIPILESNKVQIINDLVMIMKHKKIKRRVLCSEIGI
                     SYTFLSDVLNMKKNPSYDTLILIAKGIGASVRIIVEY"
BASE COUNT         2976 a         1674 c         1393 g         3815 t
ORIGIN      
        1 ccgttaggcg caataaaact tttaataatt atttccccca gagccgttac acattgataa
       61 ttccgttttt aattagaata tcttttacct tttcccaatc aacttgcggt ttatcaaaat
      121 aaaatccttc tccattttcg ccaaagtttc catacaaaag tgggcaacct aaagcagcat
      181 catcaatgta aagttgtgcg tatgctttcg ggcttgtagt ccaagtgtgt tgcgttgggt
      241 tggtttgtat accatacagc ggtatttcat tttctttaaa ccaattcaca gcatcagtta
      301 aatagttgcc actttctaaa tgaagctcat tatcttctcc agttgggtta actatatcac
      361 ttcgcattgt gaataacact aattgatgtc cgctttcaat aagcttcttt aagacaggca
      421 cagcaccaat gtctttgcca acttttggaa attcgtgcgt tacacatgtt ccgtcaaagt
      481 caatgcagat ggtaataggt gttattttct tttccatata aattttttat gtgtttttct
      541 taatccgttt atgcaggcac taatatttga cggaataaat ccgcattgtt ttgcttcttt
      601 cgcactattc cattctttta ctgggtttaa atttaggtca tattggataa ttggttggct
      661 tgacataatc ctcattgctt ctatcctttc tgctgtatat cttgttccct tcaatggatt
      721 tctatatccg cttctatatt tttctttatg ggttgcactc atttgttttt tgcttttgtt
      781 gctgaaccct tttcttttag tccaatgatt ttctccgcct tgtgcttttg actttatttc
      841 atttctccat tgcggatgtt taatgcctaa atttgctgct ctgattttat cccttgtgtc
      901 atttgttctt ttcagaccta atgaactacc agcaacccta caaatattgt aaataggctt
      961 taggttatca atgtagtgtt gctctctttc aataagtttt ttttcatcat ttacaacttc
     1021 tattatttta aactcaaagc aatctgcccc gtatttgttg taagcatttt gtaatttttt
     1081 attcgggtgt ttattatttt ttaaattata gaaatgttct ctttttcttt tttcatggtt
     1141 caacgcactg ccaatgtatt gcttattagt tattttacac tgtattgagt atattacttt
     1201 catagttgca aatatacaaa aaagttccat caaaatctat tgctattatc atattttata
     1261 taacggagcg taaaacacat tcatcacgaa gtgtggatgt gatgtaagcg gctcctttta
     1321 cacttacatt ttgtaaagtt aaaataatat tttgacaatt caataatttt gtataaattt
     1381 gcattaatgt ttcgggcata caaatatcgt ctatatccaa gtgaactaca aaaagagttg
     1441 attgccaagc atattggcag tagtcggttt gtgtataatt tagccttaga aacaaaaaca
     1501 actgcatatt taggctctaa acataatttt tccccttttg acttaattaa gcaactaccc
     1561 gaacttaaaa aagaatgcga atggttaaaa gaagttaata gccaatcctt acaacaatcc
     1621 atacaaaata tggatatcgc ttttaaaaag ttttttaaag gtgctggttt ccctaagttc
     1681 aaatctaaaa gagataggca atctttttca attccacaaa atgtaattgt agagaataac
     1741 ttattaataa tacctaaatt caaagagggc attgatattg ttttgcacag accaatcaaa
     1801 gggactataa aaagtgcaac aataagtgtt actccaacgg ggaaatactt tgtttctata
     1861 ctatgtgata ctaaaaaaga tataccaatt aaagcaccta ttgaagaaaa tacaacaata
     1921 ggaatagact taggaattaa agactttgct atcacttctg acggagaagt ttttgaaaat
     1981 cctaaatacc tacgcaaagc acaaagtaag ctgaaatatg cacaacgtaa atattcaaaa
     2041 cacaaaggta agcgtactaa aaagaaactt gccaaactat acgaagatgt agtaaacaaa
     2101 cgcaaagatt ttctgcataa aacatcaact aaactaattc gtgagaacca aacgatttgt
     2161 ttagaaacgt tagctgtgaa aaatatggtt aaaaaccaca atttagcaca agcaataaat
     2221 gatgtaagtt ggtcaacttt tgtctctatg ttagagtaca aggctgattg gtatggtaaa
     2281 aacatcctac gaataggacg tttcgctcca tcatctaaaa cttgttctaa ttgtggtaat
     2341 attaacaaag aacttacact taaagaccgt gaatggactt gtggtagttg ttctactgtg
     2401 ttagaccgag atgtaaatgc tgccataaat attaaaaatt ttgcattaaa aaatatctta
     2461 tctggaacgg ataggaaaaa tcaaggtaag ttgccaacac tagttggagc gttgaccctt
     2521 gaagcctaac ccatcgcctt tggcgtgggt gggtagttca cttggttcaa aatatgcctt
     2581 acaacttcta cattaaatcg ttccatacat aaatgattta atccaaccag taacacttgc
     2641 atttacatct tcatagattc ctatctcata atcataacct acacactcgt accatttagc
     2701 agtagttttt accgcataaa ttatagtggc atggtcacgt cttagcatcc tgcctatgtc
     2761 actatatcct aatcctataa cagatttaag gttgtaaagg acagaagcaa tggagaaacg
     2821 atacgctacg atgtcacgtg tttttcgtaa cgtaaatatc attggatacg cttcttttaa
     2881 aaataacgac aatttaacta tatctatctt atccggtgga ggattttgtc tgttccgttc
     2941 aagaagcatt aggctctcca aatactccat ctttccgata ggattaagcg ttacgaatgt
     3001 tgccggatgg attgtcattg atttctttta ttaatttaaa aataaatgtg tttgagttcg
     3061 gctctttgac cacttcatac ttttctgctt cttttatcaa cgccataaac tcgtgttcca
     3121 atggcgtatc aggagtgatg ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nntttttagt gttactttca ttgtgcatct aaattgtaat tgtttttaat ttaatttttt
     3301 caatcaactc ccaacgctac gcacattttg aaataactct ccagcgttat gttgatgttt
     3361 gtctttgtga caaacctcct aactgtcgga tatgtcaacc ctgatatggg tgccaatttg
     3421 taggcactca tatttttttc tttcattgca tctacaatat gcttgtgtgc aacgttcatt
     3481 ttttttgtaa ctttattcat agtgtgcaaa tataagtata atgttttaat tatgcaagta
     3541 tattttggtt tttattttca cttagtttaa ttatgttttg ttgtataata ttttcgtgtt
     3601 tagttattac ctttaatttg tgtttatagt ccaatgtggg gtttagtagt attttgtttt
     3661 taagcctttc tgcaacttta agttcaaaag aatacaatgt gctttctatg tcgtattgtt
     3721 ctgtattttc tatattgtaa tctaatccta atgcaagatt agatatttca cgcacagcaa
     3781 catctttatt gtgtatgaaa tcagatagtt caaaactttc gcaatcttca ctatttatat
     3841 actcaaaaaa caatagacat cttttgtttt caaacatatc aggataaaaa tttttatcta
     3901 tattattaaa gtagtttaat atattccact catttaggta aatcaaaata atacgaatta
     3961 aatggagttc tatatttgtt tctttctcta tttctaatac agtgtgatgt ttttttggtt
     4021 ttgttgtatt gttaaagcca aactctttat tgtacgtttg aagtaatatg tctcttgtaa
     4081 ttttatcggg atgtaaatta atcatcctaa ttaattcttt tgccaactta actttgtcta
     4141 ttatattaaa gtcttttaat aaagatattc tatgttttat aaattctatt ttattggact
     4201 gtatataacc attggttttg ttttttaatt cccttgcaat attatcaggg tctttatctt
     4261 taggtagagg acatatataa atattcatac ctgcttttaa taattcttca caagcattaa
     4321 atgttgcctt taatccagcg ttatctccat catataagat aacaatattt tgtgtgtatc
     4381 tctttagtgt tgtagcgtgt tctgtggtaa aagcagttcc aagtgttcct actgtgtttt
     4441 ttacaccatt gtcaaacaaa gaaataacat ctgttgcgcc ctctactact atggcttcgt
     4501 ctttttctgt aatgtgtttt tttgcaaaat acaacccaaa aagctctttt gattttttaa
     4561 atactctatt ctctgatgta ttaatatatt taggttgcat tgtttcaata tttcttcttg
     4621 cagaaaaacc aacaaccttt cctatgctat tgtgtatagg aaatattgtc ctatccctaa
     4681 acgtatcata ataagtgtta tcggctttaa ttctacataa tccatattta gatacttctt
     4741 cattggtaaa acctttttct ataaggtact tataaagttg tgagtataaa gaagatgtat
     4801 gtcctatttg aaataaacta atcacttcat cgcttaaccc tctttctttt atcagtctat
     4861 gagacaaact gtttggtagt ttatatgtaa aaaattcagt agtaacttta tttatttcta
     4921 atatcttatt ggtttcaagt tgggttgcat ctaactcatt acctccatct tcttttagtt
     4981 cgattttgta tttatcacaa agtgtggtta ttgtgtcgta aaaagataat gcttcatatt
     5041 tcattacaaa agaaataaca tcccctccct cgccacaccc aaaacactta tagaagttgt
     5101 tgttttgatt tactataaaa gatggcgttt tctcattgtg aaaaggacaa caggctctat
     5161 atataccacc ttgtttcttt aggtggatat acgaacctat cacatctaca atatttatag
     5221 acgaaactga tttaatagtg ttttgtgata ttttacccat tattggttat tagaagtgaa
     5281 tttctttgga gtatgagtat tgttgtattc atcggcttcc cttcttgggt ataacgccaa
     5341 agtaatacaa tcgtttttat ctttgttttc ttctatccat ttactgaact ttggtatgta
     5401 aacccttaat acctgaaatg acccatgctt accatttatg gtttttacat tcaatccttc
     5461 tgctgtattg ttatctttca ttattgtata ggttgttgtt aattaaaaaa gagtaatgtg
     5521 ctaaaagaat tgcatcggca gttttaagcg ttattttcat atttgggtac agccgttgag
     5581 cataattttt taagttgttc ttatgttctg ttttgctatc aaaacccttt cctgataatc
     5641 cataatgttt catccatttt tgaggactgg cttcttcaaa tggtattttg tgtgccacta
     5701 taagtcctct taaaaaaccc tttgattctc caaacttaaa agaagatgaa acaccttgtt
     5761 ttggcattga gtgtacattc tcaatcatac atttaactat atcataccta tctttagctt
     5821 cttgtataaa ttcagataaa tctgcatctg tattatcatt agttataaca ttaagtattt
     5881 ctctaccatt tgttatcgca atagcacccg aacctcctac atctattcca atatagactt
     5941 gttccttgtt ttctaagtta aactccataa taattaaatt tttggtttgt tattgttaat
     6001 ttgttctttt gacttcaaaa tactatcaga aacatcgcct ttaaacgaaa tagttcttaa
     6061 tacctttctg tctaaatttt taccctctgt tttccatgtt attttaccat attcatcaaa
     6121 atccaatgta tcaggcgttt tgtttgtaga tatattgata ttgtcgccca taaactgcct
     6181 tataatgttc tcttttagtg ttacatctct ttctaactct tttattaatt ctttgtagta
     6241 tttcaactct aatgcgttta taagttgact ttcatccgct tttataatat caggttcaga
     6301 taaccacctt tggttcataa aatctttata tcgttcagaa ccatcggcag aaggctctaa
     6361 tttttgtatt tcgtactcta tcttccttac cgcatccatg tttcctctgt tgagttctaa
     6421 ctctcgttcc gctactaatt ttttggcagg taaaaccatt ttataccaaa acacataaga
     6481 ttgctcttct atttgagata taagcgactt attggcttcg actgggtaaa cccacaactc
     6541 cctattgtca agctctaaaa aggctatttc tgcgtattct aaacccatta tcattaattg
     6601 ttgctggacc tgcattacat acatggggtc tataccctct tgccattttt tggcaaccca
     6661 agatgaccct gttttacact ctaaaattcc attatcttcc aacattgtac catcggttaa
     6721 tttaaaacag ccttttgtta ttaacctgtc tatgttcgta aataaatggg gatatttttc
     6781 attagataaa actccgtgta catttctaca atctctaact ttattgtttt tttcatagtt
     6841 gaaaatgtaa gaatctttag tgccgtcata atacttccaa gcgtttgcaa ttaactcttc
     6901 ttgttgatgc ccccaaaaag tgtatttgtt agattcccta aaggcaggtt tcaaccctat
     6961 tttctcataa aaaatctcta tacaactacc accctcgtaa tcattaagac caagtattct
     7021 gcctatatca gaagctccaa taccattact tctcctcttg tgccattctt catctttctc
     7081 tggtatgcgc tctatataca cacctctttc tttgttgtaa tattccttag aatgcaatgt
     7141 ctgtgttgtt tgtgtttgat tcattttttt tattatttaa aattgacgat aattcattgt
     7201 tataatcaaa acccccatac ccttttataa aatcaaaaaa taaacttccc gtttttccct
     7261 ccctgttttt tgctacaata acttcacatt tatttgacat ttcaattgga gttatatttc
     7321 ttccgttttt atgtaacaaa aaatatttac cctctaaatg tccgtatgta tctctttcat
     7381 ctttacttct tgcttcaaaa atattttgag tgtacatatc gggtctgtaa aacaaaacaa
     7441 ctacatctga atcattctca atagaacctg aatcttttaa atcgccaatt ataggtcgag
     7501 gattgggtct tttgtctaat tctttattta attgttgtaa tataattatt gtaacattta
     7561 gttttcttgc tatttttttt aaagcaaacg taaaataacc aatagcatcg cttttagtag
     7621 tttgctttgt ttcataatgt aattttacta aagtaagata atctattgca aagaccttaa
     7681 tgtgttttgt tatagccaaa acaataatct ttgctaaaat agagtgtaca tcgtattcgg
     7741 gattatcgtc tataaatagc ggtgatgcag aaaaagtggt aacaaaatta tgaaacctgt
     7801 ctttatcttt gtcgcctaat aaattgtttc taaaagcata cgaatttatt ttaagatgag
     7861 cagatgttat tcttgtaaca tacgcacttg ctctcatttc taatgaaaaa accgcacaag
     7921 gtatgttgtt taaaaaagat aatcttctta acacttgaag taaaaaagcc gttttccctg
     7981 ccgatgaacg tgccgcaaac gtaaccagtg ttcctggtaa taaagtaaag taatcgtcta
     8041 tttctttgta ccctgttgtg tatcctaact ctttttcgga ttcatttaac aatccaagca
     8101 tcatatcttt taataatatt ccaacaggct tagatgtatc aatattttct ccatacaaag
     8161 aactttctaa tgaatttatg gcttctaaaa ttaattcatc tggaggtatt tcgccttttt
     8221 gtgctttgtt taaaatgtct ttggctacat ctacttgaac cctatataaa tagtcttttt
     8281 ttaatacttc tatccttaat tcaagtgttg gaagactaag tgtattgctt gttatttcac
     8341 tatatacatc atcacctatt tgttgataat gattattctt tattaaccta tcttttactg
     8401 taaaaaaatc aacctctgat tggttaataa ataagtcata aatcgcagaa aaaacgtatt
     8461 gagatatttt atactcaaaa aactctttct tgtttatttt ttgaattgca ataaccttat
     8521 tgggttcatc gttgattatc gcacctaata ttgaaaattc taactttaaa tctgacatag
     8581 gtattatttt gtatttaact caatattggt atcattaata gcattaagtt ctaatctata
     8641 attatgtgtg tagtattgga ttgtttttgg gcttatgtgt cctaattttg ccgatgcttt
     8701 agtttcatca tttgtagcat taagtgtttt aaaagcggct gtataacgta tgctatgact
     8761 tgaataatcc tctaatccaa ctgcttcaaa taattgtttt agtctcttgc aaaatactgt
     8821 aacatttaaa ggtttatatc tttcgttttt attaaaatga aatttatgct gtataaataa
     8881 aggctcgttt ttatcataaa aacctcttgt tctaaggtag gtgtttatgg cttttaatgt
     8941 gccatcgtgt actctacaca ctaatcttct gccccccttt tgtgtactgt ctattattgt
     9001 gtagttattg ccaaaagcat cactaatagt cttaatgtcg cttatcttaa tacgaaatgg
     9061 ctctcctacc ctcattcctg ttgattgtag tagttttaat atggcataat ctcttatatc
     9121 tgccgatttt ctaaagtcga ataatttgta tatttctaaa aacttcttat ctgaaataac
     9181 atctttattt ccaccctcat atctatattt tttaataaac agcctattgt aaactttatt
     9241 ctcaaattca gtatgagaat ctaaatattc aaagaatttc tttaaagtgt tagacactct
     9301 gaaaacgtga gattctttgt aattataatg caaagaatta atataatctt ggatgcagtt
     9361 ccagtttaat ggctcaccac actcttttaa cattttgttt aaaacagaac gataattcat
     9421 tgcagtattt ttggctacct gtctccatgc aataaattct tctacatgtt tttctaaatt
     9481 tattgctttt acttgcaatg ggtgtagtgg tattattcta cactctttag gttgggtttt
     9541 ttcttcttgg tatttaaaat cgagattcat aatgaaagta tttaaaatag tattacattt
     9601 gcaaagataa taaaatttaa acaagtagta ttaaaaaaat gaaaaaaaat aaacacacag
     9661 atttaattcc tattttagaa agtaataagg ttcagataat taacgactta gttatgataa
     9721 tgaagcataa aaaaattaaa agacgagttt tgtgttcgga aataggtatt agttatacgt
     9781 ttttaagtga tgtattaaat atgaaaaaga atccgtctta tgatacattg attttaatag
     9841 ccaaaggtat aggtgctagt gttagaataa ttgtagaata ttaaatatgt ttatgaggaa
     9901 ataaaaataa ttatctatct ttgtaaagga aaagatacga ttgccgattt gtatcaat
//
LOCUS       DAOPNX010000093         9695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_7950_length_9695_cov_6.583921, whole genome shotgun sequence.
ACCESSION   DAOPNX010000093 DAOPNX010000000
VERSION     DAOPNX010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9695
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7950_length_9695_cov_6.583921"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..654)
                     /locus_tag="PKN53_09290"
     CDS             complement(103..654)
                     /locus_tag="PKN53_09290"
                     /inference="COORDINATES: protein motif:HMM:NF024751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HOD89182.1"
                     /translation="MKTKEDRYEKVNLFFQDESRFGLFTRNGKALTAKGIKPICPFHQ
                     VFKSLYLFGAFSPINGDNFLLEMPVCNSDTFQIFLDEFSKINANELKIMVLDNGAFHK
                     AKKLVIPDNIILIFLPPYSPELNPAEKVWAKFKRDFSNRLFLTLDALKEYITQITSLL
                     SPTEVISICAYDYIFLGTCWTNI"
     gene            complement(621..1169)
                     /locus_tag="PKN53_09295"
     CDS             complement(621..1169)
                     /locus_tag="PKN53_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89183.1"
                     /translation="MAAPKIIIVKESFEYLRKLLKSSTLFMSPRIKMLIELKKHEDTG
                     ISKRELADLIGVNHNSIQAWRTQYLKGGIDLLLSHKRIGYKPSLITAEERDKIEALLS
                     NPSNGIRGYTELHAWVEKELNKKINYNTLYKYCVREFGSGIKVARKSHIRKDMEAVEA
                     LKKTSVKSASKQSKLKKTDTKK"
     gene            complement(1373..2392)
                     /locus_tag="PKN53_09300"
     CDS             complement(1373..2392)
                     /locus_tag="PKN53_09300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD89184.1"
                     /translation="MKKVAVVILNWNGKKFLEQFLQCLVDNTPSALADIVIIDNASTD
                     DSLEYLKSNFPDIKTVVLDKNYGFAGGYNRGLKEVEAEYYLLLNSDIEVTEGWLEPLV
                     EMLDNDKSIAVCGPKLLSYYEKDKFEYAGAAGGFIDRYAYPFCRGRIFDTCETDSGQY
                     DTNIECFWISGAAFMVRAEIFNMLNGFDDEFFAHQEEIDLCWRIQNSGYKIVIQTKST
                     VYHVGAGTLPKSSPFKTYLNFRNNLYIIEKNIPKYKRNKVKFVRLFLDQVAFFKILLQ
                     GKPVEAFSICKAYFHFWKNAKKMSKHRKIVKPKALKYIKTYYNRSIVFDYYLKRIKSF
                     DKLKF"
     gene            complement(2398..3747)
                     /locus_tag="PKN53_09305"
     CDS             complement(2398..3747)
                     /locus_tag="PKN53_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOD89185.1"
                     /translation="MAKQKTGVKKQKKLFILDTNVLLHDFKCIFKFKEHDVLIPIVVL
                     EELDKFKRGNDLINFHAREFTRIIDDLAGDDFFKEGVSLGEGLGKLFVETGKPFSENL
                     QNNFPEHRPDHIILAITEYYHTNFPKQQNILVTKDINLRVKAKALGLISEDYKNDHVE
                     NLEGLNKEVITVEHVDKALISSLYEENEGVAFENFPLDIEPYPNQYLILKNGSSSALA
                     HYDSASETLNRVFKTNVYGIEPRNAEQAFAIDALLRPEILLVSLTGKAGTGKTLLALA
                     AALHQRRNFQQIFLARPIVALSNKDLGFLPGDANEKITPYMQPLFDNLSVIKHQFKPQ
                     SPDFTKIDEMLQSNKLLIEPLAYIRGRSLSKVFFIIDEAQNLTPHEIKTIITRAGEGT
                     KIVFTGDIGQIDSPYLDARSNGLSYLTTKMKGQEIFAHVNLVKGERSYLAELASDLL"
     gene            complement(3848..4684)
                     /locus_tag="PKN53_09310"
     CDS             complement(3848..4684)
                     /locus_tag="PKN53_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOD89186.1"
                     /translation="MGTYNLLKGKRGIIFGALDENSIAWKVAIRAKEEGANFVLTNSP
                     LALRRKEIFELAKKTDTQVIAADITEVSELEDLFEESMKILGGKIDFVLHSVGMSPNV
                     RKNIPYIELDYDYMHKTLDVSAISLHKVLAVAKKLDAINEGGSVVALTYIAAHRTFSS
                     YSDMANAKSMLESIVRQFGYHYGVAKNVRINSVSQSPTRTTAGSGVSGFNAFFNYANS
                     MSPLGNADADSCANTCMFLFSDLSKMITMQTIYNDGGFSSMGISNKLINQYIDAGCGE
                     DK"
     gene            complement(4753..4826)
                     /locus_tag="PKN53_09315"
     tRNA            complement(4753..4826)
                     /locus_tag="PKN53_09315"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4790..4792),aa:Ile,seq:cat)
     gene            complement(4978..7122)
                     /locus_tag="PKN53_09320"
     CDS             complement(4978..7122)
                     /locus_tag="PKN53_09320"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOD89187.1"
                     /translation="MKNILVLSFIFIFSFSAFAGFVPRKDAEKVAKSHVFQAIASFEP
                     LKWEEIRLNCLFDPSQNDIYKFYVFNINGDKGYVIVSSDDQIIPILAYSFEGGFNFDN
                     MSPGQADFLNYFNESIEFVKNNEMNVNEKAVSQWQELLYFNHNKDFQLKSTSPILLQG
                     INWNQSWPYNSQCPEDANAGYGLNGRVPVGCVATAMLQVMKYYNWPATGTGSKYHSSW
                     YNGGYGNITINFAQQTYDWASIPDQASSYVNPELGKINYHAGVAVSMGWGPSGSGTST
                     NKVSEALKNYFKYSSSAQYVAKMNYTETNWKNLIKQQVDAKKPMVYAGSPSSGAGHAW
                     NCDGYQDDSFHMNWGWGGAGNGYYTLDNLTSTATVGGPENNFNQFNEMIINIYPDTGF
                     PTYCQSEKLITGNEGSFDDGSGPQDYLANQSCVYEIKPACGSVVNLNFTKFDLADGDV
                     VELYDGDKTSSKLIAALNKDSNHAQLFSAFTGGMTIKFNTNASSQADGWKVKYTVDNC
                     KFNMLFTEAQGTLSDGSKSCDYNNSTFCSWIIEPEGANYINIDFVKCKIADNYDYLKI
                     YKDAIGADNELYKFNNTTTPSGTISIPSGKVVLQFYTNSNGVDEGWELNYTSGHSDVK
                     NNILLSEFSVIPNPGNLDSRLILTLSDYSNTKIFVTNLLGEIIAVKEYKLHSGYHEIS
                     LKEIFKDKPNSGIYFINVDSGKEIKTQKFVVF"
     gene            complement(7231..8790)
                     /gene="nadB"
                     /locus_tag="PKN53_09325"
     CDS             complement(7231..8790)
                     /gene="nadB"
                     /locus_tag="PKN53_09325"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009040691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOD89188.1"
                     /translation="MKKQFDFLVIGSGIAGLFYALKVSKFGKVAIIAKEDISNTTTVK
                     AQGGIASVMHTPDSYDKHIEDTINAGAGLNDREVVEIVVKEGPTRVFDLINIGTHFDK
                     DENGSYELGKEGGHSENRVLHFKDKTGQEIQRALINEVIRDPNIFIFENHFAIEIITQ
                     HHLGQTVKKSTPDITCYGAYIFDTKSKKVFTFLAKTTMIATGGCGNAYQNTTNPPGIT
                     GDGIAMVYRAKGVVENMEFVQFHPTALYNPNERPSFLITEALRGEGAILKNHKNEDFC
                     IKYDARGSLAPRDIVARAIDTEMKLSGEDHVFLDARHIDKEKLITNFPTIYAKCMEIG
                     INISKDLIPVTPAAHYLCGGIKTDIYARTSIINLFATGEAACTGLHGANRLASNSLLE
                     AVVFAHRAAEVAGDAKWYPSEIINKIPDWNDEGTINNEELVLISYSRKEVQQIMSNYV
                     GIVRSDLRLQRAMDRLNVIFQETEELYKVSKVRVDICELRNIINIGYLIIKQAKERRQ
                     SIGLHYNSDLP"
     gene            8911..9165
                     /locus_tag="PKN53_09330"
     CDS             8911..9165
                     /locus_tag="PKN53_09330"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOD89189.1"
                     /translation="MKDKLVNLVTYRYNHRAEILKDKLEEESIECFVTNASAFGQVIG
                     FRVMVFEKDLEEAMKVYKHIQSLYEDHSTDVLEGPEEEEE"
     gene            complement(9167..>9695)
                     /locus_tag="PKN53_09335"
     CDS             complement(9167..>9695)
                     /locus_tag="PKN53_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOD89190.1"
                     /translation="NLPSNLYYHDLKHTIDFLYVAEEIGRKEGINEEEMLLLKCAALF
                     HDAGFIVSYDNNEEIGAKMAAETLEKNGFSSTQIETVKRLIMATKMPPRPKDLLEKIM
                     CDADLDYLGRPDFIPISQNLFRELFERGKINTIDQWNKMQYKFIQNHSYFTSTAREKR
                     DAGKQLVLKELEEMV"
BASE COUNT         3161 a         1693 c         1314 g         3527 t
ORIGIN      
        1 ggcattccgg agcacttcac tatcaacctg cgataaagtt tattagatgc aataccttat
       61 aaattaccac tatccgcccg cttgatttta taccaatttg aattatatgt tagtccaaca
      121 tgtgcctaaa aaaatataat catatgcgca aatagatata acttctgtag gagaaagtag
      181 agatgttatt tgtgttatat attcttttag tgcatctaat gtcaaaaata acctgttact
      241 aaaatctcgt ttgaatttag cccaaacctt ctcagctgga tttagttctg gcgagtaagg
      301 tggcagaaaa ataagaatta tattatcagg tataactaac tttttagctt tatgaaatgc
      361 cccattgtca agaaccatta tttttagttc gttagcatta attttagaaa attcatctaa
      421 gaaaatctga aatgtatcac tattacaaac aggcatttca agaagaaagt tatctccgtt
      481 aattggtgaa aaagctccaa aaaggtaaag tgatttaaac acctgatgga atggacagat
      541 aggttttatt cctttagcag tcaaagcttt cccattccta gtaaaaagtc caaatcgact
      601 ttcgtcttga aaaaaaagat ttactttttc gtatctgtct tctttagttt tgattgcttc
      661 gatgcagatt tgactgaagt tttttttaaa gcctcaactg cttccatatc ttttctgata
      721 tgactttttc tagcaacttt tattccagag ccaaactctc taacacagta tttataaagt
      781 gtattgtagt ttattttttt attaagttct ttttcaaccc acgcatgtaa ttctgtatag
      841 cctcgaatac catttgaagg attgcttaat aaagcttcga ttttatctct ttcctcggcg
      901 gtaatcagcg aaggtttata acctattctt ttgtgggaaa gcagtaagtc tattccgcct
      961 ttcaaatatt gagtacgcca tgcttggata ctattatgat ttactcctat aaggtctgct
     1021 aactcacgtt ttgagatacc tgtgtcttca tgtttcttta attctattaa catttttatt
     1081 cgtggcgaca tgaataaggt actagacttt agtaatttac gcaaatactc aaaactttct
     1141 tttactatta taattttagg tgctgccata ttgatttata ttttagttca aaattacaaa
     1201 ataaaaatta aattacaaaa ttttttggac tattttttta atcaaaatgg tattgttaaa
     1261 ataatgtgca aaattaattt aaaacacaat caaagattta ttaattatcg tgtttgaaaa
     1321 catgcgagac ttaacctcat gagtatcaac taaatttaag aggttacaac ttttaaaatt
     1381 ttaacttatc aaaacttttt attcttttta gataataatc aaaaacaatg ctacgattgt
     1441 aataagtttt tatgtatttc agagcttttg gtttaacaat ttttcggtgc ttgctcattt
     1501 tttttgcatt tttccaaaaa tgaaaatatg ctttacaaat agaaaaagct tctacaggtt
     1561 ttccttgcaa caaaatttta aaaaacgcta cttggtcaag gaatagtcta acaaatttaa
     1621 ctttattgcg tttgtattta ggaatatttt tttcaataat atataagtta tttctaaagt
     1681 ttagataagt tttaaacgga ctgctttttg gcaatgttcc tgctcccaca tgataaactg
     1741 tgctttttgt ttgaataaca attttatatc ctgaattttg tattctccaa cacaaatcaa
     1801 tttcttcttg gtgagcaaaa aactcatcat caaaaccatt tagcatatta aaaatttctg
     1861 ctcttaccat aaaagctgct cccgaaatcc agaaacattc aatatttgta tcatattgtc
     1921 cagagtctgt ttcgcaagta tcaaaaattc ttcctctaca aaacggataa gcatacctgt
     1981 ctataaagcc tcctgctgct cctgcgtatt caaatttatc tttttcgtaa tacgacaaga
     2041 gttttggtcc gcaaactgca atagatttat cattatctaa catttctacc aaaggttcta
     2101 gccagccttc agtaacttca atatctgaat ttaaaagcaa gtaatattct gcttctactt
     2161 cttttaatcc acgattatag ccacctgcaa aaccataatt tttatctaaa acaacagttt
     2221 ttatatcagg aaaatttgat tttaaatatt ccaaagaatc atctgtagaa gcattgtcga
     2281 ttatcacaat atcagcaagt gcgcttggcg tattgtccac taagcattgc agaaattgtt
     2341 ctaaaaactt cttaccattc caatttaata tgactactgc tacttttttc aaaactttta
     2401 taataaatcg cttgctaatt cggctaaata agatctttcg ccttttacca aatttacatg
     2461 agcaaaaatt tcctgtcctt tcattttagt tgttagatat gaaagtccgt tagaccttgc
     2521 atcaagataa ggagagtcga tttgtcctat atcgccagta aaaactattt tggttccttc
     2581 gcctgcacgt gtaataatgg tttttatttc gtgtggtgtt aaattttgtg cctcatcgat
     2641 aataaaaaac actttcgaca agcttcttcc tcttatatat gctagtggct ctatcaaaag
     2701 tttattgctt tgcaacatct catcaatttt cgtaaaatca ggactttgtg gtttaaattg
     2761 atgttttatt actgataaat tatcaaaaag aggttgcata taaggagtga ttttttcatt
     2821 agcatctccc ggtaaaaatc ccaaatcttt atttgataaa gctacaatag gtcgagctaa
     2881 aaatatttgc tgaaaatttc gtctttgatg tagtgctgct gccaaagcta agagagtttt
     2941 tcctgttcct gctttaccag ttagcgacac taacaaaatt tcgggacgca acagagcatc
     3001 aattgcaaag gcttgttctg cattacgtgg ctctatgcca tatacatttg ttttaaaaac
     3061 tctgtttaaa gtttcagaag cagaatcata atgagctaaa gcacttgatg acccattttt
     3121 taaaattaaa tattgatttg gatatggctc aatatctaaa ggaaaattct caaaagcaac
     3181 tccttcattt tcctcataca aactactaat caaagcttta tcaacatgtt cgactgttat
     3241 aacttcttta tttaaacctt ctaaattttc tacatggtca tttttataat cttcggaaat
     3301 aagtcctaga gcttttgcct taactctaag gtttatgtct ttggtaacta aaatgttttg
     3361 ttgtttagga aaatttgtat gataatattc ggttattgct aagattatat ggtcgggacg
     3421 atgttctgga aaattatttt gcaagttttc agaaaaaggt tttcctgttt caacaaataa
     3481 tttgcccaat ccttcgccta agctaacgcc ttctttaaaa aaatcatcac ctgccaaatc
     3541 gtcaataatg cgagtaaact cacgtgcatg aaaatttatt aaatcatttc ctcttttaaa
     3601 cttatctaat tcttctaata caacaattgg tatcaaaaca tcatgctctt taaatttaaa
     3661 aatgcactta aagtcatgca aaagcacatt tgtatcaaga atgaaaagtt ttttttgttt
     3721 tttcacacct gttttttgct ttgccataaa aaatattttt atcgtgattt gtttacaaag
     3781 ataacatttt atttaaaaaa taaaacaata atgattttta aaaatttgtt ttatgacagt
     3841 ttagatatta cttatcttcg ccacaacctg catcaatata ttgatttatc agtttatttg
     3901 aaattcccat tgaagaaaat ccgccgtcgt tataaatagt ttgcattgtt atcattttgc
     3961 tcaaatcaga gaacaaaaac atgcaagtat ttgcacaaga gtcggcatct gcatttccta
     4021 atggtgacat agagttagca taattaaaaa acgcattaaa tcctgatacg ccacttcctg
     4081 cagttgttcg tgttggcgat tgagaaacag aatttattct aacattttta gctacaccat
     4141 aatgataacc aaattgacga acaatagatt ctaacataga tttagcattt gccatatcgc
     4201 tatagcttga gaaagttcta tgagctgcta tataggttaa agcaacaaca gaaccgcctt
     4261 cgttaattgc gtctaatttt ttagcaactg ctaaaacttt atgcaaagaa atagctgaaa
     4321 cgtccaatgt tttgtgcata taatcataat ctaattctat ataaggtata tttttacgaa
     4381 catttggcga cattccaaca gagtgtaaaa caaaatctat tttccctcct aatattttca
     4441 tagattcttc aaacaaatct tctaattctg atacttcggt aatgtcggca gcaataactt
     4501 gagtgtcagt ttttttcgcc aattcaaaaa tttcttttct gcgtaatgct aaaggagagt
     4561 ttgttaaaac aaaattagca ccttcttctt ttgctcttat agcaactttc catgcgatag
     4621 aattttcgtc taaagcacca aaaataattc cacgttttcc ttttaataaa ttgtatgttc
     4681 ccatattatt tttattattt attatttgaa ttttcagtta taataaaaaa gcctccaaaa
     4741 aagaggcttt tgtggtccca ctagggcatg atcctaggac cccctgatta tgagtcaggt
     4801 gctctaacca actgagctat gggaccgata tagataaatc tataatattt caatttatat
     4861 tcttgtttct cattaaaaac aagactgcaa agatactgct tttattttat ttttcaaaaa
     4921 aaatgctgtt ttttttctct gaatactatt tttttgcaat tttatcaatt caagatatta
     4981 aaacaccaca aatttttgtg tttttatttc ttttcctgaa tcaacattta taaaataaat
     5041 tccactatta ggtttgtctt taaaaatttc tttaagagaa atttcgtgat aacctgaatg
     5101 caatttatat tcttttacag ctataatttc accaagtaga tttgtaacaa atattttagt
     5161 gtttgaataa tcgctaagag taagtattaa gcgagagtct aagtttcctg gattagggat
     5221 aacgctaaat tctgacaata aaatattgtt tttaacatca gaatgacctg aagtgtagtt
     5281 taattcccaa ccttcatcta cgccattaga gtttgtgtaa aattgcaata ctacttttcc
     5341 gcttggtatt gaaatagttc cacttggggt tgttgtatta ttaaatttat acaactcatt
     5401 atcagcacca atagcatctt tataaatttt gagataatca taattatctg caattttaca
     5461 ttttacaaaa tctatattaa tatagtttgc tccttctggt tctataatcc aagaacaaaa
     5521 tgttgaatta ttataatcac atgatttact tccatcgctt aaagttcctt gggcttctgt
     5581 aaacagcata ttaaacttac aattgtcaac agtatatttc actttccaac cgtcagcttg
     5641 agaacttgca ttagtgttaa atttaattgt cattcctcca gtaaaagcag aaaacaactg
     5701 tgcatggtta ctatctttat ttaaagcagc aattagtttt gaactagttt tgtctccgtc
     5761 gtataattct actacatcac catcagctaa atcaaattta gtaaaattca aattaactac
     5821 agaaccacaa gctggtttaa tttcataaac acaagactga ttggctagat aatcttgtgg
     5881 tccactacca tcgtcaaaag agccttcatt gccagttatt agtttttcgc tttgacaata
     5941 tgtaggaaaa ccagtatctg gatatatatt aataatcatt tcattaaatt gattaaaatt
     6001 attttctggt cctccaactg tagctgtaga agttaaatta tctaaagtat aataaccatt
     6061 accagcacca ccccagcccc agttcatatg aaagctatca tcttgatatc cgtcacaatt
     6121 ccatgcatga ccggcacctg agctaggcga gccagcataa accataggtt ttttagcatc
     6181 aacttgttgt ttaattaaat ttttccaatt agtttctgta taattcatct ttgctacata
     6241 ttgtgctgat gagctatatt taaaataatt tttcaatgct tctgaaacct tattagttga
     6301 agtgcctgag cctgaaggtc cccatcccat actaaccgcc acgccagcat gatagttaat
     6361 ttttcccaac tctggattta cgtaacttga agcctgatct ggaatagatg cccaatcata
     6421 agtttgttga gcaaaattga tagtaatatt accatatcct ccattgtacc aacttgagtg
     6481 atattttgag ccagtacctg ttgcgggcca attataatac ttcataactt gcagcattgc
     6541 tgtagcaaca caaccaacag gcacacgtcc attcaaacca taaccagcat tagcatcttc
     6601 tggacattga gagttatagg gccaactttg attccaatta attccttgta ataatattgg
     6661 cgatgttgat tttaattgaa aatctttatt atgattaaaa tacaataatt cttgccattg
     6721 acttacagct ttttcattta cattcatttc gttattttta acaaactcta tgctttcgtt
     6781 aaaataattc aaaaaatctg cttgtcctgg cgacatatta tcaaaattaa acccaccttc
     6841 aaaagaataa gccaaaattg gaattatctg gtcatcagat gaaactataa cataaccttt
     6901 atcgccattt atattaaaaa cataaaactt ataaatgtca ttttgcgatg gatcgaaaag
     6961 acaatttaat cttatctcct cccatttaag tggttcgaaa cttgcgatag cttgaaacac
     7021 atgacttttt gctacttttt ctgcatcttt tcgaggtaca aaacctgcaa atgcactaaa
     7081 agaaaaaata aaaatgaaac ttaaaactaa tatatttttc atactttaat aaattatatt
     7141 tttgttggat acaaataaaa caatttttat tgtcataaca acaaaaaaat cataaaaaaa
     7201 tcataaaaat tacacattca attatttaac tcatggtaaa tcagaattat aatgtagtcc
     7261 tatagactgt cttctttctt ttgcctgttt aattattagg tatcctatat taataatatt
     7321 tcttagttca caaatatcca cacgtacttt tgaaaccttg tacaattctt cagtttcttg
     7381 aaaaataaca tttaatctgt ccattgctcg ttgtaatcta aggtcgcttc tcactattcc
     7441 cacataatta ctcataattt gttgtacttc ttttcgagaa tatgaaataa gcactaattc
     7501 ttcattattt atagtacctt catcattcca gtcggggatt ttattgatta tttcagaagg
     7561 ataccatttt gcatcaccag ccacttcggc agctctatgg gcaaatacca cagcttcaag
     7621 caatgagttt gatgctaagc ggttagctcc atgcaaacct gtgcaagcag cttcgcctgt
     7681 tgcaaataaa tttataatac tagtccttgc ataaatatct gtttttattc cgccacacaa
     7741 ataatgagct gcgggagtaa caggaattaa atccttgctg atatttatac ctatttccat
     7801 acattttgca taaatagttg gaaaattagt tattaatttt tctttatcaa tatgtctagc
     7861 atctaaaaaa acatggtctt cacccgacag tttcatttct gtatctattg cacgagcaac
     7921 tatgtcgcgt ggagcaagcg aacctctagc gtcatatttt atgcaaaaat cttcgttttt
     7981 atgatttttt aaaattgctc cttctcctct taaagcctct gtaatcaaaa acgatggtcg
     8041 ttcattaggg ttatacaatg ccgttggatg aaattgtaca aattccatat tttcgactac
     8101 tccttttgct ctataaacca ttgctattcc atcacctgtt attccgggtg gatttgtagt
     8161 attttgataa gcattaccac agcctcctgt tgctatcatt gtggtttttg ccaaaaatgt
     8221 aaaaactttt ttcgacttag tatcaaaaat ataagcccca taacaagtaa tgtctggagt
     8281 agattttttt actgtttgtc ctaaatgatg ctgggttata atttctattg caaaatgatt
     8341 ttcaaaaata aaaatattag gatcgcgtat tacctcatta attaatgctc tttgtatttc
     8401 ttgtccagtt ttgtctttaa agtgtaaaac acgattttca gaatgtcctc cctcttttcc
     8461 taattcataa cttccgtttt catctttatc aaaatgagtt cctatattta ttaaatcaaa
     8521 aactctagtt ggtccttctt ttacaacaat ttcaacaact tctctatcgt ttaatccagc
     8581 acccgcattt attgtatctt caatatgttt atcgtaagaa tcgggagtgt gcattacaga
     8641 agctatccct ccttgagctt taacagtagt tgtattagaa atatcttctt ttgctataat
     8701 agcaactttt ccaaatttgc taaccttcaa agcataaaat aagcctgcta ttccactacc
     8761 gataactaaa aaatcaaatt gttttttcat ataaacacaa aattccaatt aaacattttt
     8821 gcaaagataa gttaaaaacg gttttattta atattttaaa acaattttaa aaaaactacg
     8881 tataataaat taaatataaa cttaaatatt atgaaagaca agttagttaa tttagttact
     8941 tataggtata atcatagagc agaaatttta aaagacaaat tagaggaaga aagtattgaa
     9001 tgttttgtaa caaatgccag tgcatttggt caagtaattg gctttagagt tatggttttt
     9061 gaaaaagacc tagaagaagc tatgaaagtt tataaacata ttcaaagcct atacgaagac
     9121 cactcaactg atgttttaga gggacccgaa gaagaggaag aataaactaa accatttctt
     9181 caagttcttt taaaaccaat tgtttaccgg catctctttt ttctcttgca gttgaagtaa
     9241 aataactgtg attttgaata aatttatact gcattttgtt ccattgatct atagtgttta
     9301 tttttcctct ttcaaaaagt tctctaaata agttttgtga aattgggata aaatctggac
     9361 gacctaaata atctaaatct gcatcacaca ttattttttc taataaatct ttaggtcgtg
     9421 gtggcatttt agttgccata attaaacgtt ttactgtttc tatttgtgtt gaactaaacc
     9481 catttttctc taaagtttcg gctgccattt tagctccaat ttcttcgtta ttatcataac
     9541 taacaataaa tccagcatcg tgaaataaag cggcacattt caacagcaac atttcttcct
     9601 catttatacc ttcttttcta ccaatttctt cggcaacata taaaaaatca atagtatgtt
     9661 tcaaatcatg ataatacaaa tttgatggca ggttt
//
LOCUS       DAOPNX010000094         9661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_7984_length_9661_cov_7.165417, whole genome shotgun sequence.
ACCESSION   DAOPNX010000094 DAOPNX010000000
VERSION     DAOPNX010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9661
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7984_length_9661_cov_7.165417"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PKN53_09340"
     CDS             complement(<1..720)
                     /locus_tag="PKN53_09340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD89191.1"
                     /translation="MTDALGQSQVIPYLKELSNKGFEIHILSAEKQVNFQKKNELISK
                     ILTESNIFWHHINYTKNPPIVSTLKDIHALKKLAKKLHKKYNFKIVHCRSYISAFVGL
                     SLKKKFGTKFLFDMRGFYADERVDGNLWNIKNPIFKLVYDFFKRKEKIFLQNADYTIS
                     LTNAGKKIIHQKNDFENIPIEVIPCCADIEHFDYNKISTEKTNELRTKLNISKDDFVL
                     SYLGSIGTWYMLDEMLDFFKVL"
     gene            complement(752..2716)
                     /locus_tag="PKN53_09345"
     CDS             complement(752..2716)
                     /locus_tag="PKN53_09345"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOD89192.1"
                     /translation="MAVLQKIRNRAGVAIIIFVGVALFLFVIDQDTIIRWFNKSDMSL
                     AKINGKKVEFPEYNDIEYKWNNFFAMQGQRIEQDQLRNLVWEEILQKHLMSKYFEEIG
                     LALSVDEFYELYNGESAPPEYFQLITPQILKLKYANMVAKGFYQPSKFAELEYLENNN
                     SYDIEFIMLAYKNIDNESVKFSEEDLKAYYNEHQHMFMNKKRYREVDYVVYNIIPSAE
                     DSLQAVEKIKSKYDRHISGGLVPTVSEKNLSEMDLLQMGLPENFFNEEVGYLSQILFN
                     NGSYFFSELSEINYVPDSVRASHILIRPNENFTWEDCQAKADSLLALIKAGTEFELIA
                     MTNSEDDTAERGGDLDWFTYGMMVPEFNDACFYGKTGDLLTVKTDFGVHIIKITDQTS
                     PKKRVSLTSVYEEVKFSELTVNNYFTEASHFASENTTGKQFDEAIRKEQLVNYTARLR
                     DLDDKIGQTEGARELVRWAFDDKTKEGDVSVVYNFTDKFIIAKLKGIYEVGPMPFDLV
                     KERIEPKVIDNKKAEKYLADLEKDLSNKMDLNSIAEKYGIEVASANAVSFGAGYIPSL
                     GFEPNVNAVASVLDTNVLSKPFKGNNGVFVVKVVNKTTAPSKTDFSDEQNQAMINFMS
                     RLYSQDQQTDYILEALIQKAKIEDYRYRYF"
     gene            complement(2970..5837)
                     /gene="gcvP"
                     /locus_tag="PKN53_09350"
     CDS             complement(2970..5837)
                     /gene="gcvP"
                     /locus_tag="PKN53_09350"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOD89193.1"
                     /translation="MIKEKFVSRHLGPRESEYQEMLKKVGVSSLDQLIDEVVPKDIRL
                     PKKLVLDPALTEYEYLNKIKEIASKNKIYRSFIGMGYYGTATPSVILRNIFENPGWYT
                     SYTPYQAEISQGRLEALLNFQTVLTELTGLEVTNSSLLDEATAAAEAMSMMLENRSRQ
                     AVKDNKNVLFVDENIFPQTLDVIKLRAEPIGVEVVVGNFKEANLCNKTFGAIVQYPAS
                     DGKINDYTEFTNKAHESEILVCAAVDVMALVLLKSPASWGADIAVGSTQRFGIPMGYG
                     GPHAGFLSTREKYKRSMPGRIIGISIDKHGNQALRMALQTREQHIKREKATSNICTAQ
                     ALLATMAGMYVVYHGAEGMKRIANNINKAARFLAIELEKLGYKLKHKEFFDTIFVEIP
                     EGLKSKDIQAIALKNKMNFRYCCEKYVGISTDELISVEEINTILRIFATANKKDYRPV
                     EKIEDFKGFELKFKREDNIFQHKIYNSYNSESEMMRYIKRLENRDIALNRSMIALGSC
                     TMKLNSASSMLPLSWSELSNIHPFVPEDQALGYKELVAEMEKDLSIITGFAATSVQPN
                     SGASGEHSGLMVIREYHKSRNEGHRNICLIPSSAHGTNPASAVMAGMQVVVVKCDEKG
                     NVDVDDLRAKAVEHKDNLSAVMITYPSTHGVYESKIKEMINIIHENGGQVYMDGANMN
                     AQVGITNPGFIGADVCHLNLHKTFASPHGGGGPGIGSISVAKHLAQFLPGHTMVKTGG
                     ENAIKAVAAAPYGSVYVLPITFAYVKMMGEEGLTEATKIAILNANYIANELKDYYDVV
                     YTGETGRCAHEMILDLRKFRENYGVEAGDIARRLMDYGFHAPTLSFPVHETLMVEPTE
                     SESKQELDRFIEAMKAIKMECEAIKNGEFDKDDNPLKNAPHTQVECTASEWNHKYSRE
                     QAAFPLAWIKENKFWPYVGKIDSGYGDRNLVCTCEPLESYM"
     gene            6002..6631
                     /locus_tag="PKN53_09355"
     CDS             6002..6631
                     /locus_tag="PKN53_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89194.1"
                     /translation="MRLIVLNVCMIILSLNLYSQINLSLLNGQQKTLDSYVFRSDDGV
                     EYVEFTFTKPNGKVKTSYIDCESIYSLSIDGKDSVFYYPMDNEEFSLEQMSRVVNARQ
                     LAIADYKPWLATVSGLIVGSASLFIPLDPFTRLLIPVGYAITMGFVKPPKSYVEKHYP
                     QAVNDEWFVYGYQNVGRKKIFKNTLVGTLGGVFVSGVIVGTLYLVNQNN"
     gene            6764..8734
                     /locus_tag="PKN53_09360"
     CDS             6764..8734
                     /locus_tag="PKN53_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOD89195.1"
                     /translation="MLFILGLLCVSIIFAQDKRLKRADEIYEAGEYFRAITEYTKILR
                     KEKNKSAKNELYFKIGDCYFSIGDYKKARSNYRRTVKVRELEIDSKIRLAEIQIQEGT
                     YDEAIAIYQDVLEMDPNDTVAQKGIESANLAKMWSEQATRFKVEKMKEFNSKRNDFSP
                     TVDEKGGYDHVYFSSNRDEAKGKKTSFITGEKFSDLFVSKFDRKQKWSDPVPLDSLNT
                     PFDEGTPTLAEGGRKFYYTACKAEKGKKVGCQIYEAQKVDGEWMNPTNLGLVADSLSI
                     GHPTVSPDGNTIYFSGRLQGGFGGADIWYCENEGGTWSQPKNMGPAINSSGDELFPFM
                     RDDSTFYYSSTKHPTMGGLDIFVSKKNERGRWMPSENMKPPFNSNGNDFGIYYYSNED
                     KGFFTSDRKGSKQEDIYWFEKPPLVFSLSGITKDKDTEQILDSCVITLFGSDGSIFRD
                     TTSLADKERKGKFSFRLKQKTDYAFMVQKDGYFNGKARFTTDSLMFDKVFEYEILLES
                     LNKTFEIPNIEFEFGRWDLTESSKHTLDSIARILVDNPYLVIELSAHTDMIGSDASNM
                     ELSQKRANSVVEYFTNKGIPKGRLVAKGYGESRPKVIEKPDIKYPFLPKGAVLNEDYI
                     LSLSPEQQVVANQQNRRIEMRVISNDYIPSLD"
     gene            9104..>9661
                     /locus_tag="PKN53_09365"
     CDS             9104..>9661
                     /locus_tag="PKN53_09365"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOD89196.1"
                     /translation="MTEKKILIDVEDLAKFFGLREVNLSEITKAFPRLKIVVRGDILK
                     VSGEKDEIELFETKFQAILEHYHNFNRLTVDDIKQIISSDEHVAEQKMETDVLLYSVN
                     GKPIKARTKNQKKLVEEFKENDLLFAVGPAGTGKTYTAIALAVQSLKELRVKRIILSR
                     PAVEAGENLGFLPGDLREKLDPYLQP"
BASE COUNT         3243 a         1523 c         1503 g         3392 t
ORIGIN      
        1 taaaactttg aaaaaatcca acatttcatc tagcatatac caagttccga tagagcctaa
       61 gtaggataaa acaaaatcat ctttagaaat atttagttta gttcttaatt catttgtttt
      121 ttctgtcgaa attttgttgt aatcaaaatg ttctatatct gcacagcaag gaataacttc
      181 tataggtata ttttcaaaat catttttttg atgaataatt tttttgccag catttgttaa
      241 actaattgta taatcagcat tttgtaaaaa tattttttct tttcttttaa aaaagtcata
      301 tacaagttta aaaattggat ttttaatatt ccacaaattg ccgtcaactc tttcgtcagc
      361 ataaaagcca cgcatatcaa ataaaaactt agttccgaat tttttcttta ggcttaaacc
      421 gacaaaagca gaaatataag aacgacaatg aactattttg aaattatatt ttttatgaag
      481 ttttttagca agttttttta atgcatgaat atcttttaaa gtcgaaacaa ttggtggatt
      541 ttttgtataa ttaatatggt gccaaaaaat attagactca gtaagtattt ttgaaattaa
      601 ttcgtttttc ttttgaaaat ttacttgttt ttcagccgac aatatatgta tttcaaatcc
      661 tttgtttgaa agttctttta ggtatggaat aacttgagat tgccccaagg catctgtcat
      721 accgtcataa gaaatatata aaaccttatt attagaaata tctatatcta taatcttcta
      781 ttttagcttt ttgaattaat gcttcaagta tgtaatcagt ttgttgatct tgactgtata
      841 atcttgacat gaaattaatc atagcttggt tttgttcatc agaaaaatct gtttttgaag
      901 gtgcagttgt tttgtttact actttaacaa caaacactcc attatttcct ttaaatggtt
      961 tagaaagaac atttgtgtct aatactgaag caacagcatt tacatttggt tcaaatccaa
     1021 gacttgggat ataaccagca ccaaaactta ctgcattagc agaagctact tctattccgt
     1081 atttttctgc tatgctattc aaatccattt tgttagataa atctttttct aagtcagcca
     1141 aatatttttc agctttttta ttatcaataa cttttggttc aattctttct ttaactaaat
     1201 caaatggcat aggtcctact tcgtaaatac ctttaagttt tgcaataata aatttatctg
     1261 taaaattgta aactacagaa acatcacctt ctttagtttt gtcgtcaaac gcccagcgaa
     1321 caagttctct agcaccttct gtttgtccaa ttttatcatc taaatctcta agtctagctg
     1381 tgtaatttac tagttgttct tttctaatag cttcgtcaaa ttgtttgcct gtagtgtttt
     1441 cagaagcaaa gtgagaagct tcagtaaagt aattatttac agtaagttca gaaaatttaa
     1501 cttcttcata aacagaagtt agagaaacac gtttcttagg acttgtttgg tctgttattt
     1561 taataatatg aacaccaaag tctgttttta cagttaacaa atctccagtt tttccataaa
     1621 aacaagcatc gttaaattct ggaaccatca taccataagt aaaccaatct aaatctccgc
     1681 ctctttctgc tgtgtcatct tcagagtttg tcatagcaat aagctcaaat tctgttcctg
     1741 ctttgataag agctaaaaga gaatctgctt ttgcttgaca atcttcccat gtaaagtttt
     1801 cgttaggtct aattaaaata tgactagctc taacagaatc gggaacataa ttgatttctg
     1861 ataattcaga gaaaaaatat gatccattgt tgaaaagtat ttgagatagg tatcctactt
     1921 cttcattaaa aaagttttct ggtaatccca tttgtaacaa atccatttct gacaagtttt
     1981 tttcagaaac tgttggaact aaaccaccac taatatgtct gtcatatttt gatttaattt
     2041 tttcaacagc ttgtaaacta tcttctgctg atggaattat attataaaca acatagtcaa
     2101 cttctctata tcttttttta ttcatgaaca tatgttggtg ttcgttataa taagctttta
     2161 agtcttcttc agaaaattta acactctcat tatcaatgtt tttgtaagct agcataataa
     2221 attctatatc ataagaatta ttattttcca aatattctaa ttctgcgaat ttagatggtt
     2281 gataaaagcc cttagcaacc atgttagcat attttagttt caatatttga ggtgttatta
     2341 attgaaaata ttctggtgga gcactttcgc cattatacaa ttcgtaaaac tcatcaacac
     2401 ttaatgctaa tccaatttct tcaaaatatt tgctcatcaa atgtttttgc aaaatttctt
     2461 cccacactaa atttctaagt tggtcttgtt ctatacgttg tccttgcata gcaaaaaagt
     2521 tattccactt gtattcaata tcattatact ctggaaattc aacttttttc ccatttattt
     2581 ttgctaaaga catgtcgctt ttgttaaacc atcttattat tgtatcttga tcaataacaa
     2641 ataggaataa ggcaacgcca acgaaaatta ttattgctac tcctgctcta tttctaatct
     2701 tttgtaatac tgccattact ttaaatttta ataaatgtta taaactataa tcttaaaata
     2761 ttttcggttg cgaatataat aaaatattat tgtataaaaa caatgaaata aaaataatta
     2821 cgcacaattg tctaaaattt taataaaact gacattctga aatataaaaa aacgaaacag
     2881 tctcattgtg tttaaggctt ttgacctgaa aaactaataa gactgtttca aatcattaaa
     2941 aacaataaat aaaatagaaa attattttct tacatataag attctaatgg ttcgcatgtg
     3001 caaacaagat ttctatctcc atatccgcta tcaatttttc cgacataggg ccagaattta
     3061 ttttctttaa tccaagctaa tgggaaagca gcttgttcgc gtgaatattt gtgattccat
     3121 tcgcttgcag tacattcaac ttgagtatgt ggtgcatttt tcaatggatt atcatctttg
     3181 tcaaattcgc cgtttttaat tgcttcgcat tccattttaa tagctttcat agcttcgata
     3241 aatctatcta attcttgttt tgattcactt tcggttggtt caaccattaa ggtttcgtga
     3301 acagggaaag atagagtagg agcatggaag ccataatcca ttagtctgcg agcaatatct
     3361 ccagcttcaa cgccataatt ttcgcggaat tttcttaaat ctaaaatcat ttcgtgagcg
     3421 caacgaccgg tttctccagt gtaaacaaca tcgtaataat cttttagctc gttagcgata
     3481 taatttgcat ttaagattgc gatttttgta gcttcagtta atccttcttc gcccatcatt
     3541 tttacataag caaaagtaat tggcagaaca taaaccgaac cataaggtgc agcagcaact
     3601 gctttaattg cattttctcc tccagttttt accatagtat gaccaggtaa gaattgtgct
     3661 aaatgttttg ctacagaaat acttccaatt ccaggtccgc caccaccgtg aggactagca
     3721 aaagttttgt gtaaatttaa gtggcaaacg tcagctccga taaatcctgg gttggttatg
     3781 cctacttgag cattcatatt tgcaccgtcc atgtaaactt gaccaccatt ttcgtgaatg
     3841 atatttatca tttcttttat ttttgactca taaacaccat gagttgatgg gtaagtaatc
     3901 atcacggcac ttaaattgtc tttatgttct acagcttttg cacgtaaatc atcaacatca
     3961 acattacctt tttcgtcgca ttttacaaca acaacttgca ttcctgccat aactgccgat
     4021 gctgggtttg tcccgtgtgc tgaagaagga attaagcaaa tatttctatg tccttcgttt
     4081 cttgatttat gatattcacg tattaccatt aaaccagaat gttcgccact tgcaccagaa
     4141 tttggttgaa ccgaagtagc cgcaaaacct gttattatgc ttaaatcttt ttccatttca
     4201 gcaactaatt ctttgtagcc aagtgcttga tcttctggca caaaaggatg aatgttgctt
     4261 aattctgacc aactcaaagg aagcatagaa ctagcagaat ttaatttcat tgtacaagaa
     4321 cccaaagcaa tcattgaacg gttaagtgct atatcacgat tttctagtct tttgatataa
     4381 cgcatcattt cgctttcgct gttgtatgag ttgtagattt tgtgttggaa aatattgtct
     4441 tcacgtttga attttaattc aaaaccttta aaatcttcaa ttttttcaac tgggcggtaa
     4501 tcttttttat tagctgttgc aaaaattctt agaattgtat tgatttcttc aacagaaatt
     4561 aattcgtcag tagaaattcc aacatatttt tcgcaacaat atctaaagtt cattttgttt
     4621 tttaaagcaa tagcttgaat atctttagat tttaaacctt caggaatttc aacaaaaatg
     4681 gtgtcgaaaa attctttatg ttttaactta taacctaatt tttcgagttc aatagctaaa
     4741 aatctagcag ctttatttat gttgttagca attcttttca ttccttctgc cccatgataa
     4801 acaacataca ttccagccat tgtagctaat agagcttgag cagtacaaat atttgaagtt
     4861 gctttttcgc gttttatatg ttgttcacga gtttgtaaag ccattcttaa tgcttgattt
     4921 ccgtgtttat caatgctaat tccaataatt ctacctggca tgctacgttt gtatttttcg
     4981 cgagtagaaa ggaaacctgc atgaggtccg ccatatccca taggaatacc gaagcgttga
     5041 gttgaaccaa cagcaatatc agctccccaa gaagcaggcg attttaaaag taccaaagcc
     5101 attacgtcaa cagctgcaca aactagtatt tctgattcgt gagctttgtt agtaaattct
     5161 gtataatcat taatttttcc atctgatgca gggtattgaa ctattgcacc aaaagttttg
     5221 ttacaaagat tagcttcttt gaaatttcca acaacaactt ctacgccaat aggttcggct
     5281 cttaatttaa taacatctaa agtttgtgga aaaatatttt cgtcaacaaa taaaacattt
     5341 ttattgtctt ttactgcttg acgtgagcga ttttctaaca tcatgctcat tgcttctgca
     5401 gcagcagtag cttcgtctaa aagcgaagag tttgtaactt ctaaacctgt aagttctgtc
     5461 aaaacagttt ggaaatttaa taatgcctct aaacgacctt gagaaatttc tgcttgataa
     5521 ggagtgtaag aagtgtacca tcctggattt tcgaaaatat ttcttaaaat tacagaagga
     5581 gtagcagttc cataataacc cataccaata aatgatctgt aaatcttgtt tttagaagca
     5641 atttctttaa ttttattcaa atattcatac tcggttaaag ctggatctaa gacaagtttt
     5701 ttaggcaaac gaatatcttt tggaacaact tcatcaatta attggtctaa agatgaaaca
     5761 ccaacttttt tcaacatctc ttgatattcg ctttcgcgtg gtcctagatg gcgagaaaca
     5821 aatttttctt ttatcatttt attataattt tatattgtta ctaagtttaa attttaatta
     5881 aggcaaaatt aaaaataatt tccgaaaaaa ctaaatcaac ttgcgtgttt tataatataa
     5941 attgcttagt atttttttaa attacaaaaa aattataatt ttgattttta aaatcaaaat
     6001 tatgcgacta attgtattaa atgtttgtat gattattttg agtttaaatc tttattccca
     6061 aattaatctt agtttattga atgggcaaca aaaaacatta gattcatatg tattccgttc
     6121 agatgatggt gttgaatatg ttgaatttac ctttacaaaa ccaaatggaa aagtaaaaac
     6181 atcatatatt gattgtgagt ctatttattc gttgagtata gatggaaaag atagtgtttt
     6241 ttactatcca atggataatg aagagttttc tcttgagcaa atgtcgagag tggtaaatgc
     6301 tcgtcaactt gcaattgctg attacaaacc ttggcttgct actgttagtg gtttgatcgt
     6361 aggaagtgcc tctttattta ttccgttaga tccgtttaca cgtttgttaa ttcctgttgg
     6421 ttatgctata acaatggggt ttgtgaaacc accaaagtct tatgtagaaa aacattatcc
     6481 tcaagctgta aacgatgagt ggtttgttta cggttatcaa aatgtaggac gtaaaaaaat
     6541 atttaaaaat actcttgtcg gcacacttgg cggagtattt gtctctggag taattgttgg
     6601 aactctttat ttagttaatc aaaataacta aaatcaacac atataaaaat tatgcaaata
     6661 ttgatttaat taattatttt tgcactcaca aaataaaaat gcttttttaa cgtttaatgt
     6721 attttaattt gataatatta taatgagttt ttttcgaaaa atattgcttt ttattctagg
     6781 cttactttgt gtttctatta tttttgctca agataaaaga ctaaaaagag cagacgaaat
     6841 ttacgaagct ggagaatatt tcagagcaat aaccgaatac accaaaattt taaggaaaga
     6901 aaaaaataaa agtgcaaaaa atgaattata ttttaaaata ggtgattgtt attttagtat
     6961 tggcgattat aaaaaagcaa gaagcaatta tagacgaaca gttaaagtta gagaacttga
     7021 aatagattct aaaattagat tggctgaaat tcaaattcaa gaaggtactt acgatgaggc
     7081 aatagctatt tatcaagatg tgttagaaat ggatccaaac gatactgttg cacaaaaagg
     7141 tatagagtcg gcaaatcttg caaaaatgtg gagcgaacaa gctacgcgtt ttaaggtgga
     7201 aaaaatgaaa gaatttaatt ctaaacgaaa tgatttttca cctacagttg atgaaaaagg
     7261 aggttacgac catgtttatt ttagttctaa tagagatgag gcaaaaggga aaaagacaag
     7321 ctttattaca ggtgaaaaat tttcagattt atttgtttca aaatttgata gaaaacaaaa
     7381 atggtcagac ccagtaccat tagattcttt aaatacgcct tttgatgaag gaacaccaac
     7441 acttgcagaa ggaggacgaa aattttacta tactgcatgt aaagcagaaa aaggaaaaaa
     7501 agttggctgt caaatttatg aagcacaaaa agttgacgga gaatggatga atccaacaaa
     7561 tttgggatta gttgccgatt cactttctat agggcaccca actgtttcgc ctgatggaaa
     7621 tacaatatat ttctcgggaa ggttgcaagg aggctttgga ggagctgata tttggtattg
     7681 tgaaaatgaa ggcggaacat ggtctcaacc taaaaatatg ggaccagcaa taaattcatc
     7741 gggagacgaa cttttcccat ttatgagaga cgattcaaca ttttactatt caagtacaaa
     7801 acatccaaca atgggtggac tagatatttt tgtctctaaa aagaacgaaa gaggaagatg
     7861 gatgccatca gaaaatatga aaccaccttt taatagtaat ggaaatgact ttggaattta
     7921 ttactattca aatgaagata aaggcttttt tacttcggat agaaaaggtt cgaaacaaga
     7981 agatatttac tggtttgaaa aaccaccttt agtatttagc ctatcgggaa ttactaaaga
     8041 taaagataca gaacaaatac ttgattcttg cgtgattaca cttttcggaa gcgacggttc
     8101 tatttttaga gacactactt cacttgcaga taaagaaaga aaaggaaagt ttagttttag
     8161 attaaaacaa aaaaccgact atgcctttat ggttcaaaaa gatgggtatt ttaatggaaa
     8221 agcacgcttt acaacagact cgttaatgtt tgataaggtt tttgaatatg aaattttact
     8281 tgaatctcta aataaaacct ttgaaatacc aaatatagaa tttgaatttg gacgctggga
     8341 tttaacagaa tcttcaaaac atactttaga tagtattgca agaattttgg ttgataaccc
     8401 atatctagtt atagaacttt ctgctcatac agatatgatt ggttcagatg cttctaatat
     8461 ggaattaagt caaaaacgtg caaattccgt tgtggaatat ttcacaaata aaggtatccc
     8521 aaaaggaaga cttgtcgcta aaggatatgg agaatcacgt cctaaagtaa tcgaaaaacc
     8581 tgatataaaa tatccatttt tgccaaaagg agcagtttta aacgaagatt atattttaag
     8641 cttatcgccc gaacaacaag tggttgcaaa tcagcaaaat agaaggatag agatgagggt
     8701 gatttctaat gattatatcc caagcctcga ctagtcggtg ttttataagc gtccaactgc
     8761 tgcgttgctt tcgatattca aactcagtca cgtacacttc ggctttgctc agtgcaccgc
     8821 ttctggtacg ctccttcgtt ttcaatctca gatgccttgc atttgaacac ttctaaaaca
     8881 ccttgagttt ctgttcattg aaatattgtt ttgttatatt ctaagcctcg actgaagtca
     8941 gttaaaagtt cataaagttt ttaaagtttt taaagttatt taactaaaaa actaaacact
     9001 aaaaactaaa cactaaaaac ttttaactgg tttaaaaaaa tctttaaatt tccttatgtt
     9061 taacagaata tttgtgttat tttgcacaat aaataattta tgaatgactg agaaaaaaat
     9121 tttaatagat gttgaagatt tagcaaagtt ttttggactt agagaagtaa atttatctga
     9181 aataaccaaa gcttttcctc gcttaaaaat tgtggttcgt ggtgatattt tgaaagtttc
     9241 tggcgaaaaa gatgaaattg aactttttga gacaaaattt caagcaatac ttgagcatta
     9301 tcataatttt aatcgtttaa cagtagatga tattaagcaa ataatttcta gcgatgagca
     9361 tgtggcagaa caaaaaatgg aaactgatgt tttgctttat agcgtaaatg gaaagccaat
     9421 taaagctcgt acaaaaaatc agaaaaaact agttgaagag tttaaagaaa atgatttgct
     9481 ttttgccgtt ggtcctgcag gaacaggaaa aacttatact gctattgctc ttgcagtgca
     9541 atctttaaag gaattgcgag taaaaagaat tattctttct cgaccagctg tagaagcagg
     9601 cgaaaatctt ggttttttac ctggcgacct tagagaaaaa cttgacccat atcttcaacc
     9661 t
//
LOCUS       DAOPNX010000095         9406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_8259_length_9406_cov_8.400171, whole genome shotgun sequence.
ACCESSION   DAOPNX010000095 DAOPNX010000000
VERSION     DAOPNX010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9406
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8259_length_9406_cov_8.400171"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..1708
                     /gene="gltX"
                     /locus_tag="PKN53_09370"
     CDS             191..1708
                     /gene="gltX"
                     /locus_tag="PKN53_09370"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOD89197.1"
                     /translation="MKKMVRVRFAPSPTGPLHIGGVRTALFNYLFAKRNGGKMILRIE
                     DTDQTRFVEGAEEYIMESLEWCGIKFDEGIKEGGNYGPYRQSDRREIYNKYAKILIEN
                     QSVYMAFDSPEELDKLRSEAEAKGETFVYNHESRKNLKNSLALSKEQVAELLENKTPY
                     VLRFKMPENQLVELYDEIRGEIKFNTQELDDKVLVKTDGLPTYHLANIVDDHLMEITH
                     VIRGEEWLPSLPLHDLLYRAFGWTAPKFAHLPLILKPSGKGKLSKRDGDQLGFPVFPT
                     QWDSGEEIFKGYREEGYFPEAFINMLALLGWNPGTEQEIFSMDELIQVFSLDRVGKSG
                     SRFDPDKTKWFNHQYLIKKSGEELAEEFKVILDKKNINYSGFPLSRICEMLKERVNFI
                     HELWEESYFFFQAPETYDEKFLKKSYKEDSTQLLQKLIEILKNQQNFSSEEIENSVKT
                     WIESAEIGFGRVMSPFRLALVGAGKGPHVFDIVEIIGKDESIRRISALIEFVDGK"
     gene            1772..2683
                     /locus_tag="PKN53_09375"
     CDS             1772..2683
                     /locus_tag="PKN53_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89198.1"
                     /translation="MKNLLIILLIILIANACKDAAKTDSEQTVAIEENTEIQIGGTYE
                     FGDFKNKKDVFGQLLIYPDSDTSMVFHLSKNTGEPDFQIYEVMGRTLVIDNKASYQTN
                     SICENAMYSLYFEFEENFVKVVQDDEGNSDCSFSYEFRADGNFEKTNSDIPTTYTLRG
                     TTTKEFKEWVSLNVGIDFANLEKRGDVYYYNNKPYTGTAINEPKVEHYWQSIREVFQM
                     KDGKPHGKYIETSWESGSSGNYVNGLKEGRWEVYESATLIIQNYKNGLKDGEWKYYFE
                     TEENEPYRIEVYKEGVKISEQDFDVEY"
     gene            2765..3646
                     /locus_tag="PKN53_09380"
     CDS             2765..3646
                     /locus_tag="PKN53_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89199.1"
                     /translation="MSRCFKIFYLVAFIFLISLSACDIINPSEQTPSFIAVDTLKLKT
                     NGFEQGSKKHAITDCWVYVNNKLIGVFEVPFKVPVLESGMQEIQIEPGIKNSGSDSKR
                     EIYPLMKGWYVTKNLSEGAVLTLEPEFEYRNVIFDLIEDFEDIGIEFETSLNSDTTIQ
                     IISGNEAQEGKSMYFALDNERRKFECLTSKLYEIPKTGRVFLEISFKGNGHFNFGVFS
                     VENVGTGLSEVRKNVYTFKPRDTWTTIYIDLNYHVINAKGKQFRLFFTCVRPEDASTE
                     KTEIFIDNIKLTYLLTQ"
     gene            3646..5043
                     /locus_tag="PKN53_09385"
     CDS             3646..5043
                     /locus_tag="PKN53_09385"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOD89200.1"
                     /translation="MQKSKHNLKYIIFDFFAATIAWTLFYIFRKIRIENVPINLDYKF
                     YFALATIPFFWLFLHLLSGYYKVPYRKSRLHELGQTLFTTLLGVTILFFLFMLDDWVV
                     DYKQYYKLYFTLFVLQFTLTYLPRLIMTTIVNKKVHNRKLLFNTLIIGSNQKAVKLLK
                     DFESQKLSSGLKFVGFVFVEDRPSYLLEEYLPKLGGVNDIKDIISEHKIEEVVIAIES
                     SEHAKIKNIINKLHGEKVIIKVIPDMYDILIGKVRMSAIFGLPLIEINHNIMPVWQQH
                     TKRFLDIFLSLIAIILLMPVYIALSIGVKLSSPGPILYSHERIGKNGKPFKIYKFRSM
                     YVGAEKDGPALSSKNDSRVTKFGKFMRKTRLDEFPQFFNVLKGDMSLVGPRPERQFYI
                     DQITQKAPHYIHLLSVRPGITSWGQVKYGYAENVDQMIERLQYDIIYIENRSLFIDFK
                     IMIYTIKIIFQGKGI"
     gene            5201..5488
                     /locus_tag="PKN53_09390"
     CDS             5201..5488
                     /locus_tag="PKN53_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOD89201.1"
                     /translation="MTKADIVNEISKNTGIEKIVVQKTIESFMDVVKDSLIKDENVYL
                     RGFGSFIVKTRAKKVARDISKNTTITIPAHKIPAFKPAKSFVTSVKEHNKI"
     gene            5670..7247
                     /locus_tag="PKN53_09395"
     CDS             5670..7247
                     /locus_tag="PKN53_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOD89202.1"
                     /translation="MDLQTEDKQNELFIDVRSSEINIALLQNKKLIELDCEYANIEFS
                     VGDIYLGKVKRLMPGLNAAFVDIGYKKDAFLHYLDLGAGFQTLNKFLHLGLQTKQQSV
                     SKLRKIKLEPDIDKNGKIADILNVGQYVLVQIAKEPISSKGPRLSAEISLAGRNLVLM
                     PFCDKVSISHKIKSVEERTRLKKLIESIRPDNFGVIVRTVAEKKMVAELDKELSTLIE
                     KWEGIYKKLNSAKPPQLLISEVNRTTAILRDVLNSSFNQIVVNDQATYEEVKYFIEKI
                     APEKKKIVKLYTSKEPIFDFYGIEKQIKTSFGKTVPMKNGAYLIIEHTEAAHVIDVNS
                     GNRSNSEQGQEVNAFEVNMIAAEEIARQLRLRDMGGIIIVDFIDMQDEKNKKKVYECV
                     KNSMEGDRAKFHIMPLSKFCIMEITRQRVRPEMEIKTGEACPTCQGSGKITASVNLID
                     EIENNIKFILKKEKRKYLCIKTHPFVSAFLNKGWLKSIRKRWQKNYKCEIEIFPMVAY
                     TYMEYHFFDSKDEEIFY"
     gene            7267..9279
                     /locus_tag="PKN53_09400"
     CDS             7267..9279
                     /locus_tag="PKN53_09400"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOD89203.1"
                     /translation="MRLNKILKASILVICFSVFSLSNLFAQVEDPVRFKMNIEKLENS
                     TYELIFEAEIDDKWHLYGQYFDLGGPRPLYFSFDENENYRLIDSVAELGDVIHEYDDV
                     FEVNVAYFAKQAKFVQKIEVLTDKGFNITGEISGQACYDDGACVTIGDDILFEINGGA
                     SIENQETIEAKTPVIVKENKFASSTEEKSSLLGFFFLSFLFGIIGILTPCVFPMIPMT
                     ISVFMNKSEKRSRGILKAIIFGLSITLLYTLVGVIVSLTSAGANFTTVLSTHWIPNLI
                     FFVLFLVFAASLFGLFEMVLPSGLANKADAQVDKGGMLSAFFMALTTVIVSFSCTGPI
                     IGALLVEAASGNVLEPLIGMFGFGLGFALPFTFLALVPRLISKLPKSGGWLNAVKVVM
                     GFILLAFSLKYFSNIDQNYHLGILSRDVYLAIWIVIFVLLGIYLLGKIKFSHDSDVQH
                     IGFFRMLLAMVSFVFVIYLVPGLFGANLTPIAGLLPPKTSQKFDLTAQKSSSSESVGY
                     YCESPDYADVLHMAYNVNGYFDYEQAFECAKSQNLPVLLYFTGHSCSNCKKMQAEIWS
                     DPTINDYFNNKLVVAALYVDEKSIELPKEKWFSSSKDNKLKKKLGDANADIQIVNFNT
                     NTQPYYVLISPDGKVLAEPMTYELNPEKFLNFLNKGIEEFENPAEE"
BASE COUNT         3488 a         1199 c         1624 g         3095 t
ORIGIN      
        1 cttttaactt ttaactgacg tgcccttgcg ggcgagacgt aattgttcga catgggtgct
       61 gcaaagctga aaacaggagc caagcgaatt ttgcgaagtt aacccaaacg agcaaagcga
      121 gcatataatt cttaattatc attcttcatt cttaattatt ttttatcttt gcgcttctaa
      181 atattaaaat attaaaaaaa tggtaagagt acgttttgca cctagtccaa caggtccttt
      241 gcatataggc ggagttagaa cagctttgtt taattattta tttgccaaac gcaatggtgg
      301 aaaaatgatt ttaagaatag aagacacaga tcaaacgcgt tttgtcgaag gagccgaaga
      361 atatattatg gaatctttgg aatggtgcgg aataaaattt gacgaaggca ttaaagaagg
      421 tggaaattat ggtccttatc gtcaaagcga taggcgtgaa atttataata aatacgctaa
      481 aattctaatt gaaaaccaaa gtgtttatat ggcttttgat agtccagaag agttagataa
      541 attgcgttcc gaagccgaag caaaaggcga aacttttgta tataatcatg aaagcagaaa
      601 gaacttaaaa aacagtttag ctttgagtaa agaacaagtt gctgaacttc tagaaaataa
      661 aacaccttat gttttgcggt ttaaaatgcc tgaaaatcaa ttagttgaac tgtatgatga
      721 aattcgtggc gaaattaaat ttaataccca agaattagac gataaagttt tagtaaaaac
      781 agacggacta ccaacttatc atcttgcgaa tattgttgac gaccatttaa tggaaattac
      841 tcatgttatt cgtggagaag aatggttgcc ttctttgcct ttacatgatt tgctttacag
      901 agcttttggt tggacagcac caaaatttgc acatcttcct ttaattctta aaccaagtgg
      961 aaaaggaaaa ctaagcaaaa gagatggcga ccaattaggt tttcccgttt tcccaactca
     1021 atgggattct ggagaagaaa tttttaaagg ttatcgcgaa gagggatatt ttccagaggc
     1081 atttattaat atgttagctc ttttaggttg gaatccagga actgaacaag aaatttttag
     1141 catggacgaa cttattcagg ttttttcgct tgatagagtt ggaaaatctg gtagccgttt
     1201 cgacccagac aaaacaaaat ggtttaatca tcagtattta attaaaaaat cgggagaaga
     1261 acttgctgaa gagttcaaag taattcttga caaaaaaaat ataaattatt caggttttcc
     1321 tctttcaaga atttgcgaaa tgctgaaaga aagagtaaat tttattcatg aattatggga
     1381 agaatcatat ttctttttcc aagcacccga aacttatgat gagaaatttt tgaaaaaatc
     1441 atataaagaa gactcaacac aattattaca aaaacttatt gaaatcctta aaaatcaaca
     1501 aaattttagt tctgaagaaa tagaaaattc tgtaaaaact tggatagaat cagctgaaat
     1561 aggttttggt agagttatgt cgccgtttag attagctttg gttggtgcag gaaaaggacc
     1621 tcatgttttc gatatagtag aaattatcgg taaggatgaa agcataagaa gaatttctgc
     1681 cttgatagaa tttgttgacg gtaaataatt tttattttta ctataaaatt ttgtgttttt
     1741 atatatttgt cgttttataa aaatttaaaa aatgaaaaac ttattaatta ttttactaat
     1801 tattttaatt gcaaatgctt gcaaagatgc tgcaaaaaca gattctgaac aaactgttgc
     1861 tatagaagaa aatacagaaa ttcaaattgg tggcacttac gaatttggag attttaaaaa
     1921 taaaaaagat gtttttggtc aattattaat atatcctgat agcgatacaa gcatggtttt
     1981 tcatctttca aaaaatacag gagaacccga ttttcaaatt tatgaagtta tgggtagaac
     2041 tttagttatt gataataaag cgagttatca aacaaattct atatgcgaaa atgctatgta
     2101 tagtttatat tttgaatttg aagaaaattt cgtgaaagtt gttcaagacg atgaaggcaa
     2161 ctcagattgt agtttttctt atgaatttag agcagatgga aattttgaaa aaacaaactc
     2221 agatatacct accacatata cacttagagg aactacaaca aaggagttta aagaatgggt
     2281 cagtttaaat gttgggattg attttgcaaa tcttgaaaaa agaggagatg tttattatta
     2341 caacaataaa ccttatacag gtactgctat taacgaacca aaagttgagc attattggca
     2401 atcgataaga gaagtttttc aaatgaaaga cggaaaacca catggaaaat atattgaaac
     2461 tagttgggaa tcagggtctt ctggcaacta tgtgaatgga ctaaaagaag gtagatggga
     2521 ggtttatgaa agtgcaactc taataatcca gaattataaa aatggattga aagatggcga
     2581 atggaaatat tattttgaaa ctgaagaaaa tgaaccatat agaatagaag tttataaaga
     2641 gggagtaaaa ataagtgaac aagattttga tgttgaatat tagtggtaat taaaataaag
     2701 aaatttttta tacaatattg agaatgtttt tgcgttctta gttttacaga aaaataaaga
     2761 tatagtgtcg agatgtttta aaatatttta tttagttgcg tttatatttt taataagttt
     2821 atcagcttgt gatattatta atccttccga acaaactccg tcttttattg cggttgatac
     2881 gctaaaatta aaaactaatg gttttgaaca aggaagtaaa aaacatgcta ttaccgattg
     2941 ttgggtttat gtaaataata aattaattgg agtttttgaa gttccgttta aagttcctgt
     3001 tttagaatct ggtatgcaag aaatacaaat agaaccagga attaaaaatt ctggaagcga
     3061 ctcaaaaaga gaaatttacc cactaatgaa aggttggtat gttacaaaaa atttaagcga
     3121 aggagcagtt ttgacattag aaccagaatt tgaatacaga aacgtaattt ttgatttaat
     3181 agaagacttt gaagatattg ggatagaatt tgaaacaagc ttaaatagcg acacaaccat
     3241 acaaataatt tctggaaacg aagctcaaga aggcaaatca atgtattttg ctcttgataa
     3301 cgaaaggcga aaatttgaat gtttaacaag caaattatat gaaatcccaa aaacaggaag
     3361 agtgttttta gaaataagtt ttaaaggaaa cggacatttt aactttggag tattttctgt
     3421 ggagaatgtg gggacgggct tatctgaagt tagaaaaaat gtttatacat ttaaaccaag
     3481 agacacatgg acaaccattt atatagattt gaattatcat gtaataaatg caaaaggaaa
     3541 acaatttaga ttgtttttta catgtgtaag accagaagat gcaagtacag agaaaaccga
     3601 aatttttata gacaatatta aattaactta tttattaaca caataatgca aaaatctaaa
     3661 cataatttaa aatatataat atttgacttc tttgctgcta caatagcatg gacattgttt
     3721 tatatttttc gcaaaatacg tatagaaaac gttccaatta atttggatta taagttttat
     3781 tttgctttag caacaattcc atttttttgg ttgtttcttc atttgcttag tggctactat
     3841 aaagttccat atagaaaatc aagattacat gaattgggac aaactttatt tacaactctt
     3901 ttgggtgtta ctattttgtt ttttctattt atgttagacg attgggttgt ggattataaa
     3961 caatattaca aattatattt tactttattt gtcttacagt ttactctaac ttatcttcct
     4021 cgactaataa tgacaacaat agtgaacaaa aaagttcaca atagaaaatt gctatttaat
     4081 acattaatta taggaagcaa tcaaaaggct gttaaacttt taaaagattt tgaatctcaa
     4141 aaacttagtt cgggtttaaa atttgttggt tttgtatttg ttgaagatcg tccaagttat
     4201 ttgctagaag aatatttgcc aaaattgggt ggagtaaacg atattaaaga tataatttct
     4261 gaacataaaa tcgaagaagt tgtaatagca atagaatcta gcgagcatgc aaagattaaa
     4321 aacattataa ataaattaca tggcgaaaaa gttattatta aagtaattcc agatatgtat
     4381 gatatattga taggaaaagt aagaatgtcg gctatttttg gattgccttt aattgaaata
     4441 aatcacaaca taatgccagt gtggcaacag cacacaaaaa gatttttaga tatattttta
     4501 agtttaatag ctataatatt attaatgcct gtttatattg ctttaagcat aggtgttaaa
     4561 ctaagttcgc cgggtccaat tttatatagc cacgaaagaa ttggaaaaaa tggaaagcct
     4621 tttaaaattt ataaatttcg ctcaatgtat gttggtgcag aaaaagatgg tccggcttta
     4681 agtagtaaaa atgacagtag agttactaag tttggaaaat ttatgcgaaa aactcgactt
     4741 gatgaattcc cacaattttt taatgtactt aaaggcgata tgagtttagt tggtcctcgc
     4801 cccgaacgtc agttttatat cgaccaaata acacaaaaag cacctcatta tattcacttg
     4861 ttatctgttc gtcctggaat tacttcgtgg ggacaagtaa aatatggcta tgccgaaaac
     4921 gttgaccaaa tgattgaaag attacaatat gatataattt atattgaaaa tcgctcttta
     4981 ttcattgatt ttaaaataat gatttatact attaaaatta ttttccaagg aaaaggaatt
     5041 tgatttttgg tttttaaacg tttattttat caaagttttt gatacttagt tcaataatat
     5101 taaaaaaatc aaatattatt atttactttt gaaaaaagtt tatatctttg cacttgcaaa
     5161 aatgaaaata gtattaattt atttataaaa atatttagac atgacaaaag cagatattgt
     5221 aaacgaaatt tcaaaaaata ctggaattga aaaaatagtt gttcaaaaaa ctatagagtc
     5281 gtttatggat gttgtaaaag actctttaat aaaagatgaa aatgtatatt taagaggttt
     5341 tggtagtttt atagtaaaaa caagagctaa aaaagttgct cgtgatattt ctaaaaacac
     5401 tactattact atacccgctc ataaaatacc tgcttttaaa ccagcaaaat catttgtaac
     5461 atcagttaaa gaacacaata aaatttagaa attatgccaa gcggaaagaa aagaaagaga
     5521 cacaagatgt caacccacaa aagaaaaaaa cgtcttcgta agaatagaca caaaaagaaa
     5581 aaataaacct ttttcttgtt tttttgttat aatcaaacct ttgttctaca gaggtttgat
     5641 ttattaattt taaaatactg tgttatatcg tggatttgca aacagaagat aaacagaacg
     5701 aactttttat agatgtacgc agttctgaaa ttaacatagc tttactacaa aataaaaaac
     5761 taatcgagtt agattgcgaa tatgcaaaca ttgagttctc ggttggagat atttatttgg
     5821 gtaaggtcaa acgtttaatg cccggactta acgcagcttt tgttgatatt ggttataaaa
     5881 aagacgcttt tctacattac ttagatttag gagctggatt tcaaacctta aataaatttt
     5941 tacatttagg cttacaaact aaacagcaaa gtgtttctaa attaagaaaa ataaaattag
     6001 aaccagacat tgataaaaat ggcaaaattg ctgatatttt aaatgtcggg caatatgttc
     6061 ttgttcaaat agccaaagaa ccaatttcat caaaaggacc tcgactatct gccgaaattt
     6121 cattagctgg tcgtaattta gttttaatgc ctttttgcga taaagtatct attagtcata
     6181 aaataaaatc cgtagaagaa agaacaagac taaaaaagtt aatagaaagt ataagaccag
     6241 ataattttgg agttattgtt cgtactgtcg ctgagaaaaa aatggtggca gaacttgata
     6301 aagaacttag tactttaatc gaaaagtggg aaggaatata taaaaaacta aattcagcaa
     6361 aaccacctca attattgata agcgaagtta atagaactac agcaatattg agagatgtgc
     6421 ttaattcttc ttttaatcaa attgttgtaa acgaccaagc aacctatgaa gaagtaaaat
     6481 attttataga aaaaattgct cccgaaaaaa agaaaatagt aaaactttat actagtaaag
     6541 aacctatttt cgatttttat ggaatagaaa aacagattaa aacatctttc gggaaaactg
     6601 ttccaatgaa aaacggagca tatctaataa ttgaacacac cgaagctgct cacgttattg
     6661 acgtaaatag tggaaatcgt agtaattcag aacaaggaca agaagtaaat gcttttgaag
     6721 taaacatgat tgctgccgaa gaaattgctc gacaactaag actcagagat atgggcggaa
     6781 ttattattgt tgactttatc gatatgcaag acgaaaaaaa caagaaaaaa gtttacgagt
     6841 gtgtgaaaaa tagcatggaa ggcgatagag caaaatttca tataatgcct cttagcaagt
     6901 tttgcattat ggaaataact cgacaaagag taagaccaga aatggaaatc aaaactggcg
     6961 aagcttgtcc tacatgtcaa gggtcgggaa aaattacagc aagtgtaaat cttatcgacg
     7021 aaattgaaaa caatataaaa tttatcctca aaaaagaaaa acgtaaatat ttgtgtatta
     7081 aaacacatcc ctttgtttct gcatttttaa ataaaggatg gcttaaatca atacgcaaaa
     7141 gatggcaaaa aaactataaa tgtgaaatcg aaatattccc tatggtagcc tatacatata
     7201 tggaatacca ttttttcgat tcaaaagatg aagaaatatt ttattaataa ttaaataaga
     7261 gaacttatga gactaaataa aattttgaaa gctagtatat tagtaatttg cttttccgtt
     7321 tttagtttat ccaatttgtt tgcacaagtt gaagatcctg ttaggtttaa aatgaatata
     7381 gaaaagctag aaaactctac ttacgaattg atttttgaag ccgaaattga cgataaatgg
     7441 catttgtatg gtcaatattt tgatttaggt ggaccaagac ctttgtattt tagttttgat
     7501 gaaaatgaaa actatagact aatagattct gtggctgaat taggcgatgt aattcatgaa
     7561 tatgacgatg tttttgaagt aaatgttgca tattttgcta agcaagcaaa atttgttcaa
     7621 aaaattgaag ttttaacaga caaaggtttt aatataactg gcgaaattag cggtcaagct
     7681 tgctatgacg atggagcttg tgttactatt ggcgacgata ttttgtttga aataaatggt
     7741 ggagctagta tagaaaatca agaaacaata gaagcaaaaa ctcctgttat tgtaaaagaa
     7801 aataaatttg ctagttcaac agaggagaaa tcgtctttat taggattttt ctttttgtcg
     7861 tttttatttg gaataattgg aattttaact ccttgcgttt ttcctatgat accaatgaca
     7921 ataagtgtct ttatgaataa atctgaaaaa cgctcaagag gaattttaaa agcaataatt
     7981 tttggattat caataacatt attatatact ttggttggag ttatagtttc gctaacaagt
     8041 gcaggagcta attttacaac agttttaagt acacattgga taccaaattt aatttttttt
     8101 gttttattct tggtttttgc agcttcgctt tttggactct tcgaaatggt attaccttca
     8161 ggattagcaa acaaagcaga tgcgcaagtt gataaaggcg gaatgttatc tgcatttttt
     8221 atggctttaa caactgtaat tgtaagtttt agttgcacag gtccaattat tggagctttg
     8281 ttagtagaag ctgccagtgg aaacgtatta gaacctttga ttggaatgtt tggctttggt
     8341 ttaggttttg cacttccatt tactttcttg gctttagtgc cccgattgat aagcaaattg
     8401 ccaaaatctg gtggttggct aaatgctgtt aaagttgtta tgggttttat acttttagct
     8461 ttctctttaa aatatttctc aaatatagac caaaactatc atttaggaat tcttagtaga
     8521 gatgtatatc ttgctatttg gatagtaatt tttgtgctat taggaattta tttattagga
     8581 aaaatcaaat tttctcacga ctcagatgtt cagcatattg gtttctttag aatgttgtta
     8641 gcaatggttt cttttgtatt tgtaatttat ttagtgcctg gacttttcgg agctaactta
     8701 acaccaattg caggactatt gcctccaaaa acttcccaaa aatttgattt aacagcacaa
     8761 aaatcctcaa gtagtgaatc agtgggttat tattgcgaat ctcctgatta tgctgatgtt
     8821 ttgcatatgg cttataatgt aaatggatat tttgattacg aacaagcttt tgaatgtgca
     8881 aaatcacaaa acctgcctgt tttactttat tttacaggac attcttgttc taattgtaaa
     8941 aaaatgcaag cagaaatttg gtctgaccct acaataaacg attattttaa taataaactt
     9001 gttgtagcag ctttgtatgt tgacgaaaaa tcaatagaac ttccaaagga aaaatggttt
     9061 agctcgtcaa aagataataa actaaagaaa aaattaggcg atgctaatgc tgatattcaa
     9121 attgtcaact ttaatacaaa tactcagcct tattatgtgt tgataagtcc agatggaaaa
     9181 gttttagcag aaccaatgac ttatgaatta aatccagaaa aattcttaaa ctttttaaat
     9241 aaaggaattg aagaatttga aaatcctgct gaagagtaga gagttattaa ttttctaata
     9301 atatttgccc actcggttca actttacgaa ctttatgaac tttacaaact tttaactcat
     9361 ttggcttcac ctacggttaa tcacatgttg tcctacggac aaataa
//
LOCUS       DAOPNX010000096         9257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_8429_length_9257_cov_6.167790, whole genome shotgun sequence.
ACCESSION   DAOPNX010000096 DAOPNX010000000
VERSION     DAOPNX010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9257
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8429_length_9257_cov_6.167790"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..806
                     /gene="ablB"
                     /locus_tag="PKN53_09405"
                     /pseudo
     CDS             75..806
                     /gene="ablB"
                     /locus_tag="PKN53_09405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03827.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative beta-lysine N-acetyltransferase"
     gene            complement(815..2836)
                     /gene="uvrB"
                     /locus_tag="PKN53_09410"
     CDS             complement(815..2836)
                     /gene="uvrB"
                     /locus_tag="PKN53_09410"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOD89204.1"
                     /translation="MAYKLISEYKPTGDQPEAIEQLSKGIASGLKHQTLLGVTGSGKT
                     FTIANVIANFDRQVLVLSHNKTLAAQLYLEFKEFFPENAVEYFVSYYDYYQPEAYMPI
                     TDTYIEKDLSINDEIEKLRLSTSSALLSGRKDVIVVSSVSCIYGIGNPDDFHANTLQI
                     NIGQKYGRDILLRHLVDALYFRSDIDFKRGSFRVKGDTVDVFLAYHDIAIRIIFWDEE
                     VEEIYSFEPYSGEKIDNIETAKIYPANIFVTTKERINQAIIQIQDDMMAQVEYFKNMG
                     KTLEAKRLKERVSYDIEMIRELGHCSGIENYSRYFDGRKPGTRPFCLIDYFSKDFITV
                     IDESHVTIPQIGAMYGGDRSRKENLVNYGFRLPAAIDNRPLKFEEFESLMNQTIYVSA
                     TPANYELEKSEGVIVEQVIRPTGLLDPIIEVRPSLNQIDDLMNEINIRIEKNERTLVT
                     TLTKRMAEELNKYFLRFNLNCKYIHSDVETIERVEILAELRSGKIDVLIGVNLLREGL
                     DLPEVSLVAIIDADKEGFLRSERSLTQTAGRAARHLNGKVIMYADKITNSMQKTIDET
                     NRRREKQLKYNEKHNITPSQIIKAQREIIGKKAEYYSPDESKDIETLIAADPIVQYMT
                     VPELEKTIKNTKKLMQEASSNLDFVEAARLRDEMYAYEKLLEKLKQKNY"
     gene            2993..5077
                     /locus_tag="PKN53_09415"
     CDS             2993..5077
                     /locus_tag="PKN53_09415"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HOD89205.1"
                     /translation="MKYFLTLLIVLGLFTNLFSQEKEEETWHCLTDQIYWKLVDENPN
                     LLYQRQYLNEFVKEYIKNNPKSDREEVYVIPVVFHVVHNYGEENISYEQILGAVEQMN
                     KDYRKMRSDTASIHPAFKGIAADVKIEFRLARIDPWGNCTIGVVRTASEATSAGYEAA
                     KEASPTWPPDKYLNIWTVKVLGHGAAGWSYYPGTAPDGSDGIILLHNYVGVSGTSNNG
                     HGSVLTHEAGHYLNLPHTWGNSNEPGLADNCYDDDGIDDTPNTIGHTSCNLEAVTCGS
                     LDNVQNFMEYSYCYKMFTKGQADVMRATLNSWVGDRNNLKTQSNLIATGTNDGYVPQI
                     CEPISDFMTQTTMTCTGTTVVYNDLSHNTDFIGTYSWNMENASPSFSNEANPHVVYNE
                     PGVFDVSLTVENESGSDTKLRENYIKVYKPEHGKTLPYTESFESSVFPLVEGEYFNDF
                     IFENAGNSHWERANYSFSGNKSLRISNRNNDSDTRNRVHLPNIYIEDTSENLRVSFKC
                     AYGRINGNNPDKLYFYTSTHCGDSKQLVYLFTGSTLTSTYVSSYGGYVPAEEDWKTHS
                     FLINKNRLKTNNLRLIIESVAGDGKTMYIDDVKIEQAPVLSPEISSNLNVQVYPNPFV
                     NELYIQNSDYNNLLKIEISDYTGRLIVGKQYSSDFIDISSLVKDIPAGIYFLKIESEN
                     ENKVIKLVKSER"
     gene            complement(5091..5810)
                     /locus_tag="PKN53_09420"
     CDS             complement(5091..5810)
                     /locus_tag="PKN53_09420"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOD89206.1"
                     /translation="MFKYIGGFLGWLFGGCIGGVIGFFIGFFIDSISVNKSHFQEMPQ
                     SSYRNDFILSLLTLTAAIMKADGVVKKSELEYVKQFLRANFGVQQTKEALQTLKELLD
                     SNIDLDNVCTKIQYSMQHATKLQILHYLFGIANADGILHKNEIYLLEQIALKIGLSTS
                     DFNSIKSMFIEDTDSAYSILGVSKSASDEELKKAYRAMAIQHHPDKVANLGEDVQNAA
                     KQKFQKINEAWEKIKKERGIK"
     gene            complement(5820..6416)
                     /gene="coaE"
                     /locus_tag="PKN53_09425"
     CDS             complement(5820..6416)
                     /gene="coaE"
                     /locus_tag="PKN53_09425"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOD89207.1"
                     /translation="MIKIGITGLIGSGKTTISQIFEILEIPIYNTDLRAKELMHEDYE
                     IKEKIIQNFGSNSYIDNSLNKEYISKIIFSDNNARNIINSIVHPKVIDDFKNWLIEKN
                     NENYKIAGIESALLYQANIHKILDYIILVSSNDDTLSNRIMKRDNISQKQALLKIKTQ
                     KAELVQNYKVDYEIKNDKTESILLQTLNILNKLDLATK"
     gene            complement(6441..6926)
                     /locus_tag="PKN53_09430"
     CDS             complement(6441..6926)
                     /locus_tag="PKN53_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89208.1"
                     /translation="MEEVADKLKNKGGFISKKFKNPNVFVFLLFVLISTALWFLNYIN
                     KEHSTTIKVAYQFNNLPNNIVLSQENPKELNIKIKGYGYNLIKENFKRKQVAVKIDFA
                     QDNKQLKLNKLPKNKNKKFILSSELSSFFSNKFDESLTIIDISPDTIFFENVPTQSRS
                     K"
     gene            complement(6936..7247)
                     /gene="yajC"
                     /locus_tag="PKN53_09435"
     CDS             complement(6936..7247)
                     /gene="yajC"
                     /locus_tag="PKN53_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOD89209.1"
                     /translation="MNTIFLQASQQQNPYMTPIFLVLLIVVFYFFMIRPQMKRQKEMR
                     KFREGLQKGDKVITAGGIYGKVVEVQDNYLFVEIDTNVKIKVDKGSVVASPEDIQQQQ
                     K"
     gene            complement(7276..7728)
                     /locus_tag="PKN53_09440"
     CDS             complement(7276..7728)
                     /locus_tag="PKN53_09440"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOD89210.1"
                     /translation="MKANILIILSLVVLSLVACNNKNSKKTGGEYNIINTESADGNTN
                     SNLLPIIKFDELEFNFGTVIQGEVVSHTFYFTNTGKGNLIISNVKASCGCTVPKWSKE
                     PIKPNAEGSVELVFDTANREGKQIKSAKVYSNTEPNTTELIIRCEIIK"
     gene            complement(7810..8757)
                     /gene="nusB"
                     /locus_tag="PKN53_09445"
     CDS             complement(7810..8757)
                     /gene="nusB"
                     /locus_tag="PKN53_09445"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOD89211.1"
                     /translation="MISRRLIRIKAMQTYYSFLQDSDSQNLNSLQRELEYNIKQSYSL
                     FIQLHCLLINIADYATERIEIAKNKHIKSEADLNPNTKFINNIIINNLHKADQVEKTL
                     RDKTFSWNENPHFIKNLYAKIISSQYYIDYMNNDERSYKEDLNIINKIITKIIIVNPL
                     INEIIEEHSIYWNDDLEFLASNLIANLKKHDIKTIENYKLPPIFKNQEDEDFANKLIK
                     TTALHSSNYDEIIQQALEKWELERIALLDRVLLHLALAEIMEFPNLPAKVSINEYIEI
                     SKFYSTSKSSVFINGVLDKIYLELKSSGKLNKSGLGLVE"
     gene            8895..9194
                     /locus_tag="PKN53_09450"
     CDS             8895..9194
                     /locus_tag="PKN53_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOD89212.1"
                     /translation="MESNEKKRDEIYSVSVRAGRRTYFFDIKSTRQDDLYLTITESKK
                     VFGNDGNSHFKKHKIFLYKEDFGKFIEGYEQALSYIKEQKPEYFEEQDLETEEEN"
BASE COUNT         3085 a         1279 c         1392 g         3501 t
ORIGIN      
        1 tttatcaagt tttttttaaa ttaagcgtga aattttggcg gagttagggt tttaatcctg
       61 aagatgacaa ttatgaaata atagctgaaa tagaaaattt agctgaagaa aattcttata
      121 gtaaaattat ttgtctaatt cgcacagatg atttaccaga ttttatatta agtggttata
      181 aagttgaagc ttatgttcct aaattttttg atggtaaaac tgattgtatt atggtttcga
      241 aatttctcac agaacaacgt gctaaaactc caagaaagca attaggatta ttttccgatt
      301 tattggaaat tactgaagtt gaagataatt atgagaaatt gcaaaatttt agaatggaat
      361 cattaaacga atctcatgca gatgaaatag caaaaatatt acaacaaaca tttgcgtctt
      421 atccttttcc tatagaaact gaaaatatac ttaaaggaat acaagaaaac cggttgttct
      481 atttcggaat ttgggacgga gatagaatag ttggtttgtc gggagcagaa attaaccata
      541 aagaaaaaca tgcagaagta accgattttg cagtgcttcc agaatataga ggtaaaagat
      601 ttgcatttca tttattgaag tttacagaag aaaatttaaa gcaaaaaaat ataaaaacag
      661 cttatgcaat tgcaagatta tcagagttag gaatgaataa aactctttta aatgcttcgt
      721 ataaatatag tggaacttta gtaaacaata cacatatagc agggcaaatc gaaagcatga
      781 atatttatta taagcatttg aactaattat tgacttagta gtttttttgt tttaattttt
      841 ctaaaagctt ttcataagca tacatttcgt ctctaagtct tgctgcttca acaaaatcta
      901 agtttgaaga ggcttcttgc attagttttt ttgtattttt aatagttttt tcaagctctg
      961 gtactgtcat gtattgtact attgggtcag cagcaattag tgtttcaata tctttgcttt
     1021 cgtcaggaga ataatattca gcttttttac caataatttc tctttgtgct tttataattt
     1081 gactaggtgt gatattatgt ttttcattgt attttaattg cttttctcta cgtctgtttg
     1141 tttcgtcaat tgttttttgc attgaatttg taattttatc agcatacata ataacttttc
     1201 cattcaaatg tcttgctgct cttccggcag tttgagttaa tgacctttct gaacgtaaaa
     1261 aaccttcttt atcagcatca attattgcaa ctaatgaaac ttcaggcaag tctaaacctt
     1321 ctctaagcaa attcacacct attaaaacat caattttccc agagcgtaat tcagctaaaa
     1381 tttcaactct ttctatagtc tcaacatcag aatgaatgta tttacaattc aaattaaatc
     1441 ttaaaaaata tttgttcaat tcttctgcca ttctttttgt tagagttgta acaagagttc
     1501 tttcgttttt ttctattcta atattaattt cgttcattaa atcatcaatt tgatttaatg
     1561 agggtcttac ttctataatt ggatctaata aacctgtagg gcgaataact tgttcaacta
     1621 taactccttc tgatttttcc aattcataat ttgcaggagt agcacttaca taaatggttt
     1681 ggttcattaa agattcaaat tcttcaaatt taagtggtcg gttatctatt gcagcaggaa
     1741 gtctaaagcc ataatttact aaattttctt ttcttgacct gtcgccacca tacatagcac
     1801 caatttgagg aatggtaaca tggctttcat caattacagt aataaaatct tttgaaaaat
     1861 aatcaatcag gcagaagggg cgagtacctg gttttcttcc atcaaaataa cgcgaataat
     1921 tttctattcc actgcaatga ccaagctctc taatcatttc aatatcatag ctaactcttt
     1981 cttttaagcg ttttgcctcc aaagtttttc ccatgttttt gaagtactca acttgagcca
     2041 tcatatcgtc ctgaatttga attatagctt ggtttattct ttcttttgtt gtaacaaaaa
     2101 tatttgcagg ataaattttc gcagtttcta tattatctat tttttcccca gaataaggtt
     2161 cgaaagaata tatttcttca acttcttcgt cccaaaaaat tattcttata gcaatatcgt
     2221 gataggctaa aaaaacatca actgtatcgc cttttactct aaaacttcct cttttaaaat
     2281 caatatccga tctaaaatac agagcatcaa ccaaatgcct taataaaata tctcgaccat
     2341 atttttgccc aatatttatt tgtaaagtat ttgcatgaaa atcatcagga tttccaattc
     2401 cataaataca cgaaacagag ctaacgacaa taacatcttt tcttcccgaa agtaaagccg
     2461 atgaagtact taatcttagt ttttcaattt catcatttat tgaaagatct ttttcaatat
     2521 aagtatctgt aattggcatg tatgcttcgg gttgataata atcgtagtat gacacaaaat
     2581 attcaactgc attttcaggg aaaaactctt taaattctaa ataaagttga gctgcaagag
     2641 ttttgttatg acttaaaact aaaacttgac gatcaaaatt agcaataaca ttggctatag
     2701 taaaagtctt accacttccc gtaacaccta ataaagtttg atgttttaaa ccagaagcaa
     2761 tgcctttaga taattgttct atagcttcgg gctggtcgcc agttggtttg tattctgata
     2821 ttaatttata agccacagtt tttaaaataa ttgcaaagtt attaaaatta gtataaaacc
     2881 ttaaactaaa atgtttgcaa aagttaaata tttactaaaa taagtagaaa cttaaattat
     2941 atttattttt ttgtagtaaa tttgtaatat gtaaatttta ataattttta aaatgaaata
     3001 ttttttaaca ctacttatag ttttaggact ttttaccaat ttattttcac aagaaaaaga
     3061 agaagagact tggcattgtt taactgatca aatatattgg aaacttgttg atgaaaatcc
     3121 aaacttgttg tatcagcgtc agtatcttaa tgaatttgtg aaagagtata ttaaaaataa
     3181 tcctaaatct gatagagaag aagtttatgt tattcctgtg gtttttcatg tagttcataa
     3241 ttatggggaa gaaaatattt cttacgaaca aattcttgga gctgttgagc aaatgaataa
     3301 agattataga aaaatgcgtt cagataccgc ttctattcat cctgctttta aaggaattgc
     3361 tgctgatgtt aaaatagaat ttcgtttggc aagaattgac ccttggggaa attgtactat
     3421 tggtgtggtt agaactgctt ctgaagctac tagtgctggt tatgaagcag ctaaggaagc
     3481 ttcgccaaca tggcctcccg acaaatattt gaatatatgg acagtaaaag ttcttggaca
     3541 tggagctgct gggtggtctt attatccagg aacagctccc gatggaagtg atggaataat
     3601 tttattacat aattatgtgg gggtaagtgg tacaagtaat aatgggcatg gaagtgtttt
     3661 gactcacgaa gcaggacatt atttaaacct gcctcatact tggggaaatt caaatgaacc
     3721 aggtttagca gataattgtt atgatgacga tggaattgac gataccccaa atactatagg
     3781 gcacacttct tgtaatctcg aagccgtaac ttgcggtagt ttagacaatg tgcaaaattt
     3841 tatggaatat tcttattgct ataaaatgtt tacaaaagga caagctgatg taatgcgtgc
     3901 aactttaaat tcttgggtgg gagatagaaa taatctaaaa actcagtcaa atttaattgc
     3961 aacaggaact aacgatggtt atgttccaca aatttgtgaa ccaatttctg attttatgac
     4021 acaaacaact atgacatgta caggcacaac agttgtatat aacgatttgt cccataatac
     4081 tgattttata ggaacttata gctggaatat ggaaaatgca agcccaagtt tttcaaatga
     4141 agcaaatcct catgttgttt ataacgaacc tggagttttt gacgtttcgt taactgttga
     4201 aaatgaaagt ggtagcgata ctaaactaag agaaaactac attaaagtat ataaacccga
     4261 acatggtaaa actttgcctt ataccgaaag ttttgaatct tctgtttttc cattagtaga
     4321 aggtgagtat tttaatgact tcatatttga aaatgcagga aattcccatt gggaaagagc
     4381 aaactatagc ttttctggaa ataaatcatt gagaatttct aatagaaata atgactcaga
     4441 tactagaaat agagtgcatc ttccaaatat atatatagaa gatacaagtg aaaatttaag
     4501 agttagtttt aaatgtgctt atggacgaat taatggaaat aatcccgata aactttactt
     4561 ttatactagt acgcattgtg gagattctaa acaacttgtt tatcttttta caggatctac
     4621 attaacatct acttatgttt cttcgtatgg tggatatgtg cctgctgaag aagattggaa
     4681 aactcacagt tttttaataa ataaaaacag attgaaaact aataatttgc gtttaataat
     4741 tgaatcagtt gctggagatg gaaaaactat gtatattgat gatgtgaaaa ttgaacaggc
     4801 accggtgtta agtcctgaaa tttcgagtaa tttgaacgtt caagtttatc caaatccttt
     4861 tgtaaatgaa ctttatattc aaaattccga ttataataat ttgttaaaaa ttgaaattag
     4921 tgactatact ggtaggttaa tagttggaaa acaatatagt tctgatttta tagatatttc
     4981 atcacttgta aaagatattc ctgcgggtat ttacttttta aaaatagaat ctgaaaatga
     5041 aaataaggtt ataaaacttg ttaaatcaga gagataaaat ttgtaaaatt ctattttatt
     5101 cctctttctt tttttatttt ttcccaggct tcatttattt tttggaattt ttgtttagct
     5161 gcattttgca catcttcgcc taaatttgca actttgtcgg ggtgatgttg tatagccatt
     5221 gctctgtatg cttttttcaa ttcttcgtcg ctagcagatt tagaaacccc taaaatacta
     5281 taagccgaat cagtgtcttc aataaacatt gattttattg aattgaaatc gctagttgaa
     5341 agtccaattt ttaaagcaat ttgttctaat aagtatattt catttttatg aagtatgcca
     5401 tcagcattag caatgccaaa aaggtagtga agtatttgca atttagtagc atgttgcata
     5461 ctgtattgta tcttggtgca aacattatct aaatcaatgt tggaatctaa taattctttt
     5521 aatgtttgta aagcttcttt agtttgttga actccaaaat ttgctcttaa aaattgtttt
     5581 acatattcta attctgactt tttaactaca ccatctgctt tcataattgc agcagttaag
     5641 gttaaaagac ttaatatgaa atcatttctg tatgagcttt ggggcatctc ttggaagtga
     5701 gatttgttga cacttattga gtcaataaaa aaaccaataa aaaaaccgat tactccacca
     5761 atacatccgc caaataacca gcccaaaaat ccacctatat atttaaacat tccactcatt
     5821 tatttagtag ctaaatctaa tttgtttaaa atatttaaag tctgtaataa aatagactca
     5881 gttttgtcgt tttttatttc ataatcaact ttatagtttt gtactagttc agctttttgt
     5941 gttttaattt ttaataatgc ttgtttttga gaaatattat cacgtttcat tattctgtta
     6001 cttaaagtat cgtcattact gcttactaaa atgatgtaat ctaaaatttt gtgaatatta
     6061 gcttggtaaa gtaaagctga ctcaatacca gctattttgt aattttcatt atttttttca
     6121 attaaccaat ttttaaaatc atcaataact tttgggtgga ctatagaatt aattatgttt
     6181 ctagcattgt tgtctgagaa aattatttta gaaatatatt ctttgtttaa actattatca
     6241 atataggagt ttgagccaaa attttgaata attttttctt taatttcgta gtcttcgtgc
     6301 attaattctt ttgctcgcaa atcggtgtta taaattggta tttcaagtat ttcaaaaatt
     6361 tgtgaaatag ttgttttgcc agaacctatt aaacctgtta tgcctatttt aatcatgcct
     6421 tgcgtaagaa tacaaaagtt ttatttagat cttgattgtg ttgggacgtt ttcgaaaaat
     6481 atggtgtcag gagaaatgtc tataatggtt aagctttcgt caaatttatt actgaaaaat
     6541 gaactcaatt cagagctaag gataaatttt ttgtttttat tctttggtag cttatttagc
     6601 ttgagttgtt tattgtcttg cgcaaaatct attttaacag ctacttgttt tcttttaaaa
     6661 ttctctttta taagattata gccgtatccc tttattttaa tatttaattc tttcggattt
     6721 tcttgactta aaacaatatt gtttggaagg ttgttaaatt gatatgctac tttaattgta
     6781 gtagaatgtt ctttgtttat gtagtttaga aaccataaag ctgttgaaat aagtacaaat
     6841 aataaaaaaa caaaaacatt tggattttta aattttttag aaataaatcc acctttattt
     6901 ttgagtttat cagctacttc ttccatcaag tacaattatt tttgctgttg ttgaatgtct
     6961 tctggagaag caactacaga acctttgtca actttaattt taacgttagt atcaatttct
     7021 acaaataagt aattgtcttg aacttcaacg acttttccat aaattccacc tgcggtaatt
     7081 actttatcac ctttttgtaa tccttctcta aattttctca tttctttttg tcttttcatt
     7141 tgtgggcgta tcataaaaaa gtaaaagaca acaattaaaa gaacgagaaa tataggagtc
     7201 atatatggat tttgttgttg gctcgcttgt aaaaaaatag tattcatagt tttaattttt
     7261 taagttatta attatttatt ttattatttc acatctaatt attaattctg ttgtatttgg
     7321 ctcagtatta gaatatactt ttgccgattt aatttgtttt ccttctctgt ttgcagtgtc
     7381 aaaaacaagt tcaacacttc cttcagcatt aggtttaata ggctcttttg accactttgg
     7441 gacagtgcaa ccacaagaag cttttacatt tgatattatt aaattacctt ttcctgtatt
     7501 ggtaaaataa aaagtgtgcg aaacaacttc tccttgaatt actgtaccaa aattaaattc
     7561 caattcatca aattttatta taggtagtaa gttgctattg gtattgccat cagcagattc
     7621 agtgtttata atattatatt caccaccagt ttttttgctg tttttattat tgcatgcaac
     7681 taaactcagt acaactaaac ttaatattat taatatattg gctttcataa gtctaaaata
     7741 ataatgtgca aagttaataa aataaattaa ctttcattta ttttaactat atttttggaa
     7801 tttaaaatct tattcaacta aacctaaacc acttttattt agttttcccg aactttttag
     7861 ttctaaatat attttatcta aaactccatt aataaaaaca ctacttttac tagtgctata
     7921 aaatttagaa atttcaatat attcgttaat gcttacttta gctggtaagt ttggaaattc
     7981 cataatttcg gctaatgcta aatgtaaaag aactctgtct aaaagtgcaa ttctttctaa
     8041 ttcccatttt tctaaagctt gttgaattat ttcatcatag ttagagctat gtagtgctgt
     8101 tgttttaatg agtttattag caaaatcttc atcttcttgg tttttgaata ttggtggaag
     8161 tttataattt tctatggttt ttatatcatg tttttttaaa tttgcaatga gattactagc
     8221 taaaaattca agatcatcgt tccaatatat gctatgctct tcaataattt cattaattaa
     8281 tggattaact attattattt ttgtgataat tttattaata atattaagat cttctttata
     8341 acttctttcg tcattattca tgtagtctat gtaatattgg gaagaaatta tttttgcgta
     8401 caggttttta atgaaatgtg gattttcatt ccatgaaaaa gttttatctc taagagtttt
     8461 ttctacttga tcagctttgt gtaaattatt tataattata ttatttataa attttgtatt
     8521 gggattcaaa tcggcttctg acttaatatg tttgttttta gcaatctcaa tcctttcagt
     8581 agcatagtca gcaatgttta ttagcaagca gtgtagttgg ataaataagg agtaagattg
     8641 tttgatgttg tattctaatt ctctttgtaa tgagtttaaa ttttgggaat ctgaatcttg
     8701 caaaaacgaa taataagttt gcattgcttt tattctaatc aatctgcgac taatcatttt
     8761 aaagtacttt ttaaatttta ggcaaaaata ataaatatta gggataaaac tttttaactg
     8821 gaaaaaaaat attcataatt tcttgttttt ttaaaaaatt taatatattt gtataaataa
     8881 taaaactgat ttgtatggaa agtaatgaga aaaaaagaga tgaaatttat tctgtttcgg
     8941 ttcgtgcagg gaggagaaca tatttttttg acataaaatc aacacgtcaa gatgatttgt
     9001 atcttacaat tacggagagt aaaaaagtgt ttggtaatga cgggaactct cattttaaga
     9061 aacataaaat ctttttatat aaggaagatt ttggtaaatt tatagaagga tatgagcagg
     9121 ctttgagtta tattaaagaa cagaaacccg aatattttga agaacaagat ttagaaacag
     9181 aggaagaaaa ttaagtgtta aattcctgag ttccgcactt ttttgggaca ccaaagctaa
     9241 tccgttgtta atcagcg
//
LOCUS       DAOPNX010000097         9072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_8610_length_9072_cov_7.311079, whole genome shotgun sequence.
ACCESSION   DAOPNX010000097 DAOPNX010000000
VERSION     DAOPNX010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8610_length_9072_cov_7.311079"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(286..3252)
                     /locus_tag="PKN53_09455"
     CDS             complement(286..3252)
                     /locus_tag="PKN53_09455"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017142418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HOD89213.1"
                     /translation="MTTEYQIEEKLIHQLRELKYSYNPDIVDRKTLEQNFKKKFEALN
                     RVNLSDNEFLRLRDEIITPDVFLASKLLREKQYFQREDGTPLHYTLVNINDWCKNDYE
                     VVNQLRINTENSHHRYDVILLVNGLPLVQIELKKYDVSPRKAMQQIVDYKSDAGNGYT
                     NSLMCFMQLFIVSNSTKTYYFANNNKNHFAFNADEQFLPVYELADVNNKKINRLEDFA
                     EKFLKKCTLGEMISKYMILVESEQKLLIMRPYQIYAVKAIDECVKQNRGNGYIWHTTG
                     SGKTLTSFKASTLLKSNNDIEKCLFVVDRKDLDRQTREEFNKFQEGSVEENTNTETLV
                     RRLLSTDYADKVIVTTIQKLGLALDDTYKRNYKERLEPLRNKRIVFIFDECHRSQFGE
                     NHKAIREFFPNAQLFGFTGTPIFDENATYSIREGEYASYKTTQDIFEKQLHAYTITHA
                     IDDKNVLRFHIDYFKGKGNDNPKPGEPIAQQAIVEAIVEKHDAATNSRRFNALMATAS
                     INNAIEFYQLFKEVQKKKQNENPDYIPLNIACVFSPPAEGNKDIQQIQEDLAQEKEDN
                     KHEPEAKKAALKTIIEDYNVQFGTNHNINDFDLYYQDIQKRIKDHKYSNKDYPRENKI
                     DITIVVDMLLTGFDSKYLNTLYVDKNLKYHGLIQAFSRTNRILNDTKPYGNILDFRSQ
                     QEAVNQAIALFSGEESEKARKIWLVEPAPVVIDKYKTAVEKLGFFMQENNLLNEPQEV
                     YNLKGDAARIAFVRNFKEVQRLKTQLDQYTDIDEVQKAKIEAILPEEKLLEFRSSYIE
                     TAKQLRTIQEKEGDQAPPEIQELDFEFVLFASAIIDYDYIMNLIADNTQKKPAKQQMT
                     KAQIISLLSSSSNLMDEQEDLTEYINSLDWNSGQDVDNLRKGYDKFKDDKYNKELAEI
                     ANKNGLETKDLKVFVEQIINRMIFDGEKLTDLFEPLELGWRERKEKELALMADLIPLL
                     KKLAQGRDISGLGAYE"
     gene            complement(3370..4617)
                     /locus_tag="PKN53_09460"
     CDS             complement(3370..4617)
                     /locus_tag="PKN53_09460"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOD89214.1"
                     /translation="MKNPDVIVVGGGAAGLLASIRAAMGGHKVLVLEKMQTAGKKMLI
                     TGKGRCNITNSDSIKDFITQVFPDGRFLYSAFNQFFNSDILALLEDCGVETVLERGGR
                     YFPKSEKASDVVDALYKRAKDLGVEFIFNAKVLSIEQLEGKINSVTYLNNNLEEIKIP
                     VQNVIVCTGGKSYPGTGSTGDGYVFAKKLGHKVEKLLPTLVPVETFEDTASEMQGLSL
                     KNINASLIIDGIEEDSEFGELLFTHYGLSGPVILTLSRKIVVALEEKKEVKISIDFKP
                     ALDFEKLDARILRDLNEHGKMKFANLFKLWLPQKAISVFQKLSNIDIEKQASKISGAE
                     RKKIVSLLKNLEFTVKAHRGYNEAVVTAGGVCTDEVNPKTMESKLVQGLYFAGEVLNL
                     DANTGGYNLQIAFSTGWLAGGLG"
     gene            4727..5752
                     /gene="tdh"
                     /locus_tag="PKN53_09465"
     CDS             4727..5752
                     /gene="tdh"
                     /locus_tag="PKN53_09465"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOD89215.1"
                     /translation="MKALVKAKPERGLWMQDMPIPSIGPDDVLIKIRKTAICGTDLHI
                     YRWDEWSQKTIKTPMIIGHEYVGEIADAGANVKHKFTIGERVTGEGHIACGECRNCHR
                     GWDHICANNYGVGVHRDGCFAEYLSIPATNVVRISEKIEDDLCSIMDPFGNATHTALS
                     FNLLGEDVIITGAGGPIGAMCVAIAKFAGAKSVFAVEPNDYRRELAKKMGADVCVNPL
                     KEDLQEVLKKHNLIGFDIGLECSGNGGAFNQMVTAMYNGGSIALLGILPSTSPVNWNE
                     IIFKGLKLKGIYGREMFETWYKMEAMLLAGLDIKPVITHKFNVDDYLKGFEVMDSGNC
                     GKVILEW"
     gene            complement(5758..7809)
                     /locus_tag="PKN53_09470"
     CDS             complement(5758..7809)
                     /locus_tag="PKN53_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD89216.1"
                     /translation="MKIRKLIQPIVIAVFAFLLYSNTLGHKYALDDKAVYWKNEFVQK
                     GISGIKDILAYDSMAGMFGKDSKELEGGRYRPLSLITFALEVEFFGRETTDVAAKQPF
                     LGNPGISHFLNILLYIISLLILYKVLNRLFRDYKPKYEFLSIPFLATMLFAAHPLHTE
                     IVANIKGRDEILAFLFSIAALNSTLNYVDNKKFTGLISAFIYIFLGSMSKEISITFLA
                     VIPLSIYFFREEKFSKLVVLTIPLIAGAGLYMILRSLAIGDQTAIEASMLMNNPFLGT
                     TFAERYATVFLTLLLYLKLLVFPHPLTWDYYPYHIPIVGWTDWRAILSLILYLALFVI
                     AIYGLKKKTIYSYGILIYLATLSITSNLFFNIGAFMSERFVYVSLLGFCIIFAYLISE
                     KLAQKITHIKKYKNLSIGIVCVVLLAFSIKTYSRNFVWKDNLTLFGHDVHVSVNSAKG
                     NSTYASELYKLAEDAEQAQDTLLRDKYLREAIPYFEKALEIYPDYSEALVRLGNIYYK
                     LDGDYKKMLEYYLKTLETQPLEADVWANTKGVLNFNVDEPEYEKYIWKRFSELAPDYY
                     ESYFQLGSLYYFGGDNQADSAIYWLEKSKTLHPNHFENLFYLGVSYGNINDFQKAREN
                     LLLAVEISPNIEAYRYLGISYGMEGDDKTALSYFEKALALDPNNEVMKAYVELAKSRL
                     N"
     gene            7957..8679
                     /locus_tag="PKN53_09475"
     CDS             7957..8679
                     /locus_tag="PKN53_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89217.1"
                     /translation="MKTIFVKTHFLILTVVFLFIKIGNTYSQSDYTDDYQRFTISANF
                     AVQLAEYGELNNKLAEKGFSKFNQINFLTSGDFCIYYPKPKLSVHANVSGYSQVINQQ
                     NNINTSLKNTGIGVLLGYTFFSDKKIQLVPQIGTQFSWLNLDITSDIADNSTFTNYFS
                     GDANQYGMTASNLLANVGILAKTSFLINENFSNKLVVGIKTGYSMPVLKTIWTVNEKT
                     LNDGPTINPGGFYAGVVVGVEF"
     gene            8685..9038
                     /locus_tag="PKN53_09480"
     CDS             8685..9038
                     /locus_tag="PKN53_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89218.1"
                     /translation="MRKYVLILFLSTLFNCLHAQELGIKLGSSFIKNNSTINKPANIG
                     IYYNEKISDKINISTTYLHSIKKQENAEIDFQTNFKGHNIFIDFLYDVNIKNNFYIKF
                     GLGSGYSAYNNVSSM"
BASE COUNT         3050 a         1490 c         1305 g         3227 t
ORIGIN      
        1 tattttaatt attggttgta gtttgtactt tttttataga aatagacttg actgctgttt
       61 tactttttag cactcctgat aaactagaaa ccctttgttt tgtgaaagta aaaggaagag
      121 ttttgcgtcc tccatattga cttgatatca caaaatgtga cctcaagttt ctcattatta
      181 cattatgtgt agtggttgtc gcaaattttg cgacagttga aattttttct gattcgtaat
      241 tcgttatttt taatttatca tttgtcattt ctaactcgta atttatcatt catacgctcc
      301 taatcctgaa atatcacgtc cttgtgctaa tttttttaat agagggatta aatctgccat
      361 taaagccaat tctttctctt tacgttctct ccagccaagt tctaatggtt caaacaaatc
      421 tgtaagtttt tcgccgtcaa aaatcatacg gtttataatt tgttctacaa aaacttttaa
      481 atctttagtt tctaatccgt ttttatttgc aatttcagcc agttctttat tgtatttatc
      541 atctttaaat ttgtcgtaac cctttcgcaa attatctaca tcttgtccgc tgttccaatc
      601 caaactgttg atatattcgg ttaaatcttc ctgttcgtcc atcaggttgg aactagagct
      661 tagtaagcta ataatctgtg ctttagtcat ctgctgttta gccggtttct tttgcgtatt
      721 atcggcaatc aagttcataa tatagtcgta atcaataatt gccgatgcaa aaagtacaaa
      781 ttcaaaatcc aattcttgta tttcaggcgg tgcttgatct ccctcttttt cctgtattgt
      841 tcttaactgc ttagccgttt ctatatatga actgcgaaat tctagtaatt tttcttccgg
      901 caaaattgcc tcaattttag ctttttgcac ttcatctata tcggtatatt ggtctaattg
      961 agtttttaat ctttgtactt ctttaaagtt tctcacaaac gcaattctag cagcatcacc
     1021 tttcaaatta taaacttctt gcggttcgtt taataaatta ttctcctgca taaagaagcc
     1081 taatttttca acggcagttt tatatttatc aatcacaacc ggtgcaggtt caactaacca
     1141 aatctttctt gctttttcgc tctcttctcc tgaaaataaa gcaatagctt gatttacggc
     1201 ttcctgctga gaacgaaaat ctaaaatatt tccgtaaggt ttggtgtcgt ttaatattct
     1261 attagtgcga gagaatgcct gaatcaatcc atgatatttt aaatttttat caacatacaa
     1321 agtgttcaaa tattttgaat cgaaacctgt tagcaacata tccaccacta tggtaatatc
     1381 tattttattt tcacgaggat aatctttgtt gctgtatttg tggtctttta ttcgcttttg
     1441 aatatcctga taatataaat caaaatcgtt tatattgtga tttgtaccaa attgcacatt
     1501 ataatcttca ataatagttt ttagtgcagc tttttttgct tcgggttcat gtttattatc
     1561 ctctttctct tgtgctaaat cttcttgtat ttgctgtatg tctttatttc cttctgccgg
     1621 aggagaaaac acacaagcaa tattcaaagg aatatagtca ggattttcgt tttgcttttt
     1681 tttctgaact tctttgaata gctgataaaa ctcaatagca ttattaatcg atgctgttgc
     1741 cattaaagca ttgaatcgtc ttgaatttgt tgctgcatcg tgtttttcta caatagcttc
     1801 aacaatcgct tgttgagcaa tgggttctcc cggcttggga ttatcattgc ctttgccctt
     1861 aaaataatca atatgaaaac gcaatacatt tttatcatct atagcatgtg ttattgtata
     1921 ggcgtgtaat tgtttttcaa aaatatcttg tgtggttttg tatgaggcat attctccttc
     1981 tctgatgcta taagtagcgt tttcatcaaa aataggtgta ccagtaaaac caaacaattg
     2041 agcgtttggg aagaattctc ttattgcttt gtgattttct ccaaattgtg aacgatggca
     2101 ctcatcaaat atgaatacaa tacgtttatt gcgaagtggc tctaaacgtt ctttgtaatt
     2161 ccttttgtat gtgtcgtcta aagctaatcc taatttttgt atggtagtaa caattacttt
     2221 atcagcataa tcggtggaaa gcaatcgtct aactaatgtt tcggtattgg tattttcttc
     2281 tacacttcct tcttgaaatt tattaaattc ttctcgtgtt tgtcggtcaa ggtctttacg
     2341 gtcaactaca aacaaacact tttcaatatc attattactt ttcaacaaag ttgatgcttt
     2401 aaaagaagtt aaagttttgc cacttcctgt ggtgtgccat atatagccgt ttcctctatt
     2461 ttgcttaacg cattcatcaa ttgcttttac tgcataaatt tgatatggtc tcattataag
     2521 caatttttgt tcgctttcga ccaaaatcat atatttacta atcatttcgc caagtgtgca
     2581 tttcttgaga aatttttcgg caaaatcttc taaacgatta attttcttat tatttacatc
     2641 tgctaattca taaaccggaa gaaattgctc atctgcatta aaagcaaagt gatttttgtt
     2701 gttgtttgcg aaataatatg tttttgtact gttacttaca ataaacaatt gcataaaaca
     2761 cattaaagag ttagtatatc catttcctgc atcactttta taatccacaa tttgttgcat
     2821 tgcttttcgt ggcgaaacat catatttttt taattcaatt tgcactaaag gcaagccatt
     2881 aaccaacaat ataacatcat aacgatgatg actattttct gtgtttatac gtaattgatt
     2941 aacaacctca tagtcatttt tacaccaatc attaatattt accaaagtat aatgtaaagg
     3001 cgttccatct tcacgttgaa aatattgttt ttcgcgtaag agttttgaag caagaaaaac
     3061 atcaggagta ataatttcat ctcgcaagcg taaaaattca ttatcggaaa gatttacacg
     3121 attaagtgct tcaaacttct ttttaaaatt ttgctcaaga gttttacggt caactatatc
     3181 agggttatag ctatatttta actctctaag ttgatgaatt aacttttctt ctatttgata
     3241 ttccgtagtc attaatcttt ttttaatttg atttatttct atcaaattat ttttggcaaa
     3301 aaacaattta tctcgcaaat atataacatt ttgttatttt ttaaaacatc tttatgtttt
     3361 tccccctcat caccccaatc cccccgctaa ccagcctgta gaaaatgcta tttgtaaatt
     3421 atagcctcca gtatttgcat ctaaatttaa aacttcgcca gcaaaatata aaccctgtac
     3481 taattttgat tccatagttt taggattaac ttcgtctgtg caaacacctc cagctgttac
     3541 aactgcttcg ttatagcctc tgtgtgcttt tacagtaaat tctaagtttt taagcaacga
     3601 aactattttt ttacgttctg ctcccgaaat tttactggct tgtttttcta tgtcaatatt
     3661 cgataatttt tgaaaaacag aaattgcctt ttgtggtaac cacaatttga ataaattagc
     3721 aaatttcatt tttccatgtt cattcaaatc acgtaaaatt ctggcatcta atttttcaaa
     3781 atccaaagca ggcttaaaat ctatagaaat ttttacttct tttttttctt ccaatgcaac
     3841 aacaatttta cgactcaaag taagaataac tggtcccgac aagccataat gtgtaaacaa
     3901 aagttcacca aattcagaat cttcctcaat accgtcaata attaaacttg catttatgtt
     3961 ttttaaactc aagccctgca tttcacttgc tgtatcttca aaagtttcga caggaaccaa
     4021 agtaggcaat aatttttcaa ctttatgacc taatttttta gcaaacacat aaccatctcc
     4081 tgttgagcca gttccaggat aagactttcc tccagtacaa acaataacat tttgcacagg
     4141 aattttaatt tcttctaaat tattattcaa ataagttaca gaattaattt tcccttctaa
     4201 ttgctcaata gagagaactt tagcattaaa aataaattca actcccaagt cttttgctct
     4261 tttgtacaat gcatcaacaa catcagaagc tttttcggat tttggaaaat aacgtccacc
     4321 tctttccaac acagtttcaa cgccacaatc ttctaacaaa gctaaaatat cagaattgaa
     4381 aaattgatta aatgctgaat ataaaaaacg tccatcagga aaaacttgag ttataaaatc
     4441 ttttatagaa tcggaattag taatattgca acgacctttt cctgtaatta gcattttttt
     4501 tcctgccgtt tgcatttttt ccaaaaccaa aactttgtgt ccgcccatag cagcacgaat
     4561 agaagccaac aagcctgcag ctccgccacc aacaactatt acatcagggt ttttcatgtc
     4621 aaattttttg taaagatatg aattttaaaa aatttaattt cagaaatatt ttaaaaacat
     4681 aaaaaaagca aaatataatt tatatttttg gggaaaattt tatgaaatga aagcactagt
     4741 aaaagcaaaa cctgaaagag gcttgtggat gcaagacatg cctattccaa gtataggacc
     4801 agacgatgtt ttaataaaaa ttagaaaaac cgcaatttgc ggaaccgacc ttcatattta
     4861 tagatgggat gagtggtcgc aaaagacaat aaaaacacct atgattattg gacacgaata
     4921 tgttggcgaa attgcagacg ctggagcaaa tgtaaaacat aaatttacta taggcgaaag
     4981 agttactggc gaaggacata ttgcttgtgg agaatgtaga aattgtcata gaggctggga
     5041 tcatatttgt gcaaataatt atggtgtagg cgttcatcgt gacggttgct ttgcagaata
     5101 tttatcaatt cctgcaacta atgttgttag aattagcgaa aaaatcgaag acgacctttg
     5161 ctctattatg gatccatttg gaaatgcaac tcacaccgca ttgtcgttta atcttcttgg
     5221 cgaagatgta attatcacag gtgctggcgg accaattgga gcaatgtgtg ttgctattgc
     5281 taaatttgct ggagcaaaaa gtgtatttgc tgttgagcct aacgattatc gcagagaatt
     5341 agcaaagaaa atgggtgccg atgtttgcgt aaatccacta aaagaagatt tacaagaagt
     5401 tcttaaaaaa cataatttaa taggatttga tattggttta gaatgctctg gaaatggtgg
     5461 tgcttttaac caaatggtta ctgctatgta taatggaggc tctattgctt tgttgggaat
     5521 tttgccttca acttcaccag ttaattggaa cgaaataatt tttaaaggac taaaactaaa
     5581 aggtatttac ggcagagaaa tgtttgaaac ttggtacaaa atggaagcaa tgcttttggc
     5641 aggattagat ataaaaccag ttatcacaca taaatttaat gttgacgact atttaaaagg
     5701 ctttgaagta atggacagcg gaaattgcgg aaaagttatt ttagaatggt aaaattatta
     5761 attcaatctg cttttcgcta attctacata agctttcatt acttcattat taggatccaa
     5821 agcaagagct ttttcaaaat aagaaagtgc ggttttatca tcaccttcca ttccataact
     5881 aatacctaaa tagcggtatg cttcaatatt tggagaaatt tcaactgcta ataaaagatt
     5941 ttcgcgagct ttttgaaaat cattgatatt accataagaa acacccaagt aaaataaatt
     6001 ttcaaaatga tttggatgta aagttttaga tttttctaac caatatattg cagaatcggc
     6061 ttgattatct cctccaaaat aatataatga gccaagttga aaatatgatt cataataatc
     6121 tggagctaat tctgaaaatc ttttccaaat atatttttca tattcaggtt catcaacatt
     6181 aaaatttaaa acaccttttg tatttgccca cacatcagct tctaaaggtt gggtttcgag
     6241 tgttttaaga taatattcca acattttttt ataatcacca tctaatttat agtatatatt
     6301 tccaagtcta accaaagctt ctgaataatc aggataaatt tctaacgcct tttcaaaata
     6361 tggaatagct tctcgcaaat atttgtctcg caacaaagta tcttgtgctt gctctgcatc
     6421 ttctgctaat ttatacaatt cactagcata agtgctattg ccttttgcag aatttacact
     6481 tacatgcaca tcgtgtccaa ataaagttaa attatctttc cacacaaaat ttcgagaata
     6541 tgtttttatg gaaaatgcaa gcaaaactac acaaacaatt ccaatagata aatttttata
     6601 ttttttaata tgagtaattt tttgagctaa tttttcagaa attaaatatg cgaaaattat
     6661 gcaaaaacct aataatgaaa catatacaaa acgctcactc ataaatgctc ctatattaaa
     6721 aaacaagtta gaagttattg aaagtgtggc taaataaatt aaaattccat aagagtaaat
     6781 ggtttttttc tttaatccgt aaattgcaat tacaaacaaa gctaaatata aaattagtga
     6841 caaaattgct ctccaatctg tccaaccaac aattggaata tgatatggat agtaatccca
     6901 tgttaaagga tgtggaaaaa ccaatagttt taaatacaac aataaagtta aaaatactgt
     6961 tgcgtatctt tcggcaaacg tagttcccaa aaatggattg ttcattaaca ttgaagcctc
     7021 aatagctgtt tggtctccaa tagcaagaga acgcaaaatc atatataaac ctgctcctgc
     7081 aattaaagga atggttaaaa caacgagttt tgaaaatttt tcttctctaa agaaataaat
     7141 acttagagga attacagcta aaaaagtaat gcttatttct tttgacattg agccaagaaa
     7201 aatataaata aaagcagaaa tcaatcctgt aaacttctta ttatcaacat aattcaaagt
     7261 tgaatttaaa gctgctattg aaaataaaaa agctaaaatt tcgtctcttc ctttaatgtt
     7321 tgcaacaatt tcggtatgca aaggatgagc agcaaaaagc attgtcgcca aaaaaggaat
     7381 acttaaaaac tcatatttgg gcttataatc tctaaacaaa cgatttagca ctttgtaaag
     7441 gattaataaa gaaataatgt aaagcaaaat atttaaaaaa tggctaatcc caggattgcc
     7501 caaaaatggt tgttttgctg caacatcagt agtttcacgt ccaaaaaatt caacttccaa
     7561 agcaaaagta attaatgata aaggacgata acgaccacct tctaattctt tagaatcttt
     7621 accaaacatt cctgccatag aatcataagc caaaatatct tttataccag aaattccttt
     7681 ttgtacaaat tcatttttcc aatatactgc tttgtcgtct aatgcatatt tatgtcccaa
     7741 ggtatttgag taaagcaaaa atgcaaaaac ggcaataaca ataggctgaa taagttttct
     7801 aattttcatt caaaaatatt tatcaattaa tttaactttt tgcaataata gtttctaagc
     7861 aaagctataa aatttttaat aatttcggta ataataataa taattattat tattttccgt
     7921 ttaagttagt agtaataact taaatttata aaaacaatga aaacaatatt tgtaaaaacg
     7981 cattttttaa ttttaacagt ggttttttta tttattaaaa ttgggaacac ttactcacaa
     8041 agcgactata ctgatgatta tcaaagattt acaatatctg ctaattttgc tgttcagctt
     8101 gcagaatatg gtgaattaaa caacaaactt gctgaaaaag gattttcaaa atttaatcaa
     8161 ataaattttt taacaagtgg tgatttttgc atatactatc caaagccaaa attatcagta
     8221 catgcaaatg tttctggtta cagtcaagta ataaatcagc agaacaatat taacacaagt
     8281 ttaaaaaaca caggaatagg agttttgtta ggctatacat tttttagtga taaaaaaata
     8341 cagttagttc cacagatagg gacacaattt tcgtggttaa atcttgatat cacaagtgat
     8401 attgcagata attcgacatt tactaactat ttctctggag atgctaatca atatggcatg
     8461 actgcttcaa atttattggc taatgttgga atacttgcaa aaacttcttt cctcatcaac
     8521 gaaaattttt ctaataaact tgttgttgga ataaaaacag gatattctat gcctgttctc
     8581 aaaacaattt ggacagttaa tgagaaaacc ttaaatgatg gtcctactat aaatcctggt
     8641 ggtttttatg caggcgttgt tgttggagtt gagttttagg gagtttgaga aaatatgttc
     8701 taatattatt cttgtccaca ctatttaatt gcctgcacgc tcaagaatta gggattaaac
     8761 ttggaagtag ttttattaaa aacaattcta caataaacaa acctgctaat attggcattt
     8821 attataacga aaaaatatct gacaaaataa atatttctac aacttacttg cattcaataa
     8881 aaaaacaaga aaatgcagaa atagattttc aaacaaattt taaaggacat aatatcttta
     8941 tagatttttt atatgacgta aatattaaaa ataactttta tataaaattt ggtttaggtt
     9001 caggatattc agcatataat aacgtgagtt cgatgtaaga aactaaaaaa aacattaaaa
     9061 tattttgaaa at
//
LOCUS       DAOPNX010000098         8985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_8727_length_8985_cov_5.261030, whole genome shotgun sequence.
ACCESSION   DAOPNX010000098 DAOPNX010000000
VERSION     DAOPNX010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8985
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8727_length_8985_cov_5.261030"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..2002)
                     /locus_tag="PKN53_09485"
     CDS             complement(83..2002)
                     /locus_tag="PKN53_09485"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HOD89219.1"
                     /translation="MFAVLVFSTANLFSENYIIKSIDIQTDFAIVEGINSKISLVAKD
                     SLGNIADINCVREIKLNNKVFNIVFVDGIAEFEYIFNEKTDLNIQCEGIEASKTIKPI
                     PLWMSIIPPLIAILFALIFKEVFSALFIGLFSGTFIIWIYSGTNVILSIFKAILTIPD
                     TYVLRALFKTEHLSIIIFSMLIGATVQIITKNGGMKAVINILSRRANNPRSGQFVTWL
                     LGLVIFFDDYANTLVVGNTMRPVTDKLKISREKLAYIVDSTAAPVASIAFVTTWIGAE
                     LSYIQNSITQLGLNESAYNIFFSSLKYSFYPILTLIFILILIYAGRDYGPMLTAERRA
                     REKGVTAADMEQKLQTDLKEMEVSDHIKPNIYNAIIPIAIIIFGTIAGLFYTGWNPAV
                     WENKSIGFITKISETIGASDSYKALIWGSLGGLISAMFLSLSKKKLGLQNTMDSMING
                     FKAMLSAIVILILAWSLAALISDLHTAEFITSAIQSANMSPYLLPVLSFVISALMSFS
                     TGTSWGTMAIMYPLVLPATWFLCQETGMDYNASLMLFSHVVSTVIAGSVFGDHCSPIS
                     DTTILSSLASSCNHIQHVRTQLPYALTVAVVGLVFGTLPVAYGLSPWIAYPILIGVCF
                     LIIRFFGKKVEPVKQ"
     gene            complement(2029..3759)
                     /gene="lysS"
                     /locus_tag="PKN53_09490"
     CDS             complement(2029..3759)
                     /gene="lysS"
                     /locus_tag="PKN53_09490"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOD89220.1"
                     /translation="MDYIGLSDLEIQRRESLKELRNLGIDPYPAAEYHVDIYSTDILE
                     TFSDENMPENLKSVSLAGRIMSRRIMGNASFAELQDSKGRIQLYFKRDDVCPGEDKTM
                     YNVVFKRLLDIGDIIGVKGYAFRTQMGEISVHVKEFTVLTKSLRPLPIVKEKDGHVFD
                     AVTDLEMRYRQRYVDLIINPHVKDVFVKRTKIVNTMREFFNSKGYLEVETPILQPIPG
                     GAAARPFVTHHNALNIPLYLRIANELYLKKLIVGGFDGVYEFAKDFRNEGMDRTHNPE
                     FTVLEIYVAYKDYKWMMEFTEEMVERVAIALHGTTKVQLGDRIIDYKTPFARVSMYDA
                     IKKYTGYDIAGKSEDELRQICKELDIEIDDTMGKGKLIDAIFGEKCEDHFIQPTFITD
                     YPIEMSPLTKKHRSIPGLTERFELIVNGKELANSYSELNDPIDQKERFEDQLKLSEKG
                     DDEAMFIDRDFLRALEYGMPPTAGMGIGIDRLTMFMTNSPSIQDVLFFPQMKPEKKAE
                     IFNESVFVEKGIPEEWAKILYKSGFTSLAQLKEANVGKLHQILCGWNKKHKLGLQNPT
                     PEQVKEWTNF"
     gene            complement(4102..5034)
                     /locus_tag="PKN53_09495"
     CDS             complement(4102..5034)
                     /locus_tag="PKN53_09495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD89221.1"
                     /translation="MEISVVVPIYNEEGNIPQMYERLTKVLKEICSSYEIIFVNDFSK
                     DNSLSIIKDLSKKDNSVQYISFSRNFGHQVAVSAGLDYSKGDAVAIIDGDLQDPPELI
                     KEMYAKYKEGYKVVYAKRSARKGVNIFKKAAYKLFYRILDKLTEVKIPLDTGDFRLID
                     RVIINHLKQMPERNKFLRGQIAWIGYKQTCVEYVRDPRFSGKTGYSFSKLMKLAIDGI
                     TGFSSRPLKFASNMGIVVSVFSFCIIVYALISHYLLHTPPLRGWTSVIISVSFIGGVQ
                     LITIGIIGEYISRINNDVRKRPLYIVEESSEEEK"
     gene            complement(5040..6527)
                     /locus_tag="PKN53_09500"
     CDS             complement(5040..6527)
                     /locus_tag="PKN53_09500"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOD89222.1"
                     /translation="MEKKISNKKLTWILVVIFVLISLYPLFAFLGTQPLRTWDESRVA
                     ASSYEMTKTKNPIVVTYDYKPDHWSVKPPLAIWIQALSIKVFGANEVAVRLPSALAIL
                     LMGVVIILLTGVIKKPFLGFYAASVIICSRGFFIYHSGRSADYDALLCFFMIAYLLLF
                     FIYTETKEKKYYILFFVSLIFATLTKGIQALMPLPFIPIYLIFRKQLLPILKDKTTYI
                     GIGLFILFVGGYYIGRELSYGGYLKAVYENELGGRFMTVLENNSGGKCYYINNLKTNY
                     FTYFFYILPLAFIFTLFVKDKQVKRLSIFSMSIAIMQYVFLLIAKTKLHWYLLPLMPL
                     FALVIGSAFYVIHNWIINLKYKTSKIYILIIVAYIAVFSIPYKRIVKHIYRPKEDPLY
                     QAYYSRSELMKSLLKGNIVVNDTINYINGDYDQSSMFYFYAMNHYDIHNQRKNIEDLK
                     LGDLVQINEIETDEKIMERFEYEVLHFKIQSKIVRLTKLLETQNM"
     gene            7107..>8985
                     /locus_tag="PKN53_09505"
     CDS             7107..>8985
                     /locus_tag="PKN53_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HOD89223.1"
                     /translation="MKKILGLDLGTNSIGWALVNESETENEQSSIVKLGVRVNPLTVD
                     EISNFEKGKSITTNAERTLKRGARRNLQRYKLRRENLIEILRENKIIADKTILSEDGK
                     DTTHQTLKLRAKAAKEKVSLEEFAKILLLINKKRGYKSNRKAQSSDEGQLIDGMAVAK
                     ELYEKDITPGEFSYNLLKNGKNNLPDFYRSDLQMEFDRIWKFQHKFYPEILTDDLKNN
                     LKNKAKKDTWAICQKPFNIVGIKRNTKGAEQKLENYKWRKDALTKQLELEELAIVLQE
                     INGQINSSSNYLGDISDRSKELYFNNQTVGEYQMAQLVANRHQSLKNQVFYRQDYLDE
                     FEKIWETQAIHHKELTPELKKEIRDVVIFYQRPLKSQKGLLSFCEFESKEIEILINGV
                     KKKKTTGLRVCPKSSPLFQEFRIWQVLNNIEVKGVTKKNKNNIEPTLFEDNFNEKKLI
                     QKVQRPLEQEEKELLAERLMLCDKMTKNQMLTALGLNNYDMNFKEIKGNTTFAKLFQA
                     YKEIAEIVGYDKDKIKNLEDINIIFKDLGIKTDFLSFNSELDRKELQQQSLYKLWHLI
                     YSYESDNSATGNESLIRKISELCGMPEDYAKILANIVFESDYSSLSAKAIGKILPHLK
                     DG"
BASE COUNT         3288 a         1496 c         1299 g         2902 t
ORIGIN      
        1 attattaaag cagatgaaaa tatctattac ctaaaaattt agggtttgag tttaagtttt
       61 ttactaaaaa taagacaaat tattattgct taactggctc aacttttttg ccaaaaaatc
      121 taataattaa aaagcaaact ccaattaaaa tcggatatgc gatccaagga cttagtccat
      181 atgccactgg tagagtacca aaaactaaac caactacagc aactgtcaat gcataaggca
      241 attgagttct aacatgttga atatgattac aagaagaagc caaagagctt aaaattgttg
      301 tatctgaaat tggtgagcaa tggtcgccaa ataccgaacc tgcaataacg gttgatacaa
      361 catgagaaaa aagcattaaa cttgcattgt aatccattcc tgtttcttgg cataaaaacc
      421 atgttgctgg taaaaccaaa ggatacatta ttgccattgt tccccacgat gtgcctgtgc
      481 taaacgacat tagagcagaa ataacaaaac ttaaaacagg taaaagatat ggactcatat
      541 ttgcagattg tattgctgat gtaataaatt cagcagtatg taaatctgaa attagggcag
      601 caagcgacca tgccaaaatt aaaattacaa ttgccgacaa catagcttta aagccattaa
      661 tcatgctatc cattgtgttt tgcaagccaa gttttttctt acttaaactc aagaacattg
      721 cagaaatcaa tcctcctaaa gaaccccaaa tcagagcttt ataagaatct gaagctccta
      781 ttgtttccga aatttttgtt ataaatccta tagatttatt ttcccaaaca gcaggattcc
      841 aacctgtgta aaaaagacct gcaatagttc caaatataat aattgcaata ggaataatag
      901 cgttataaat gtttggctta atatggtcag aaacttccat ttcttttaaa tcggtttgca
      961 gtttttgttc catatctgct gctgtaacac ctttttctct tgctcgtctt tctgctgtta
     1021 acattggacc ataatctctg cctgcataaa ttaaaatcag tataaaaatt agagttaaga
     1081 ttggataaaa agaatattta agacttgaga aaaagatatt ataagcactc tcatttaatc
     1141 caagttgagt gatagaattt tgaatatatg aaagttctgc tcctatccat gttgtaacaa
     1201 aagcaataga agccacaggt gctgcggtgc tatcaacaat ataagctaat ttttctcgcg
     1261 aaattttaag tttatccgtt actggacgca ttgtatttcc tactactaaa gtatttgcgt
     1321 agtcatcaaa aaatattact aaacctaaca accaagtaac aaactgccct gagcgaggat
     1381 tattagcacg ccgagataaa atatttatca ctgctttcat tccaccattt tttgtaataa
     1441 tctgcacagt agcaccaatt aacatagaaa aaatgattat agacaaatgc tcggttttaa
     1501 aaagagcacg caagacataa gtatcaggaa ttgtaagtat tgctttaaaa attgaaagga
     1561 taacatttgt gccagaatat atccaaatta taaaagtccc actaaacaaa cctatgaata
     1621 aagcagaaaa aacttcttta aaaattaaag caaacaatat tgcaatcaat ggtgggataa
     1681 ttgacatcca aagaggaatt ggctttatag ttttgcttgc ttctatcccc tcgcactgaa
     1741 tgtttaaatc tgttttctca ttaaaaatat actcaaattc tgcaattcca tcaacaaaaa
     1801 ctatgttaaa tactttatta tttaatttga tttcacgtac acagttgata tctgcaatat
     1861 ttccaaggct atctttagct accaaactaa tttttgaatt aatgccttca acgattgcaa
     1921 aatcggtttg tatatctatt gattttatta tatagttttc tgaaaacaaa tttgcagtac
     1981 taaaaacaag tactgcaaac aaaactagta tatttttgag tttaaacatt agaagttagt
     2041 ccattcctta acttgctcag gtgttggatt ttgcaagcca agtttatgtt ttttattcca
     2101 accgcacagt atttgatgca attttcctac attagcttct tttagctgag ccaaagaagt
     2161 aaagcctgat ttgtataata tttttgccca ttcttctggt atgccttttt caacaaaaac
     2221 agattcatta aaaatttctg cctttttctc aggtttcatt tgtgggaaaa acaaaacatc
     2281 ttgaattgat ggactattag tcataaacat tgttaatcta tcaattccaa ttcccattcc
     2341 tgcggttggt ggcattccat actctaaagc acgtaaaaaa tctctatcaa taaacattgc
     2401 ttcatcatcg cctttttcgc ttagtttaag ctggtcctca aatctttctt tttggtctat
     2461 tgggtcattt aactcagaat aagaatttgc aagttcttta ccgtttacaa taagttcaaa
     2521 tctttcggtt aagccaggaa ttgaacgatg tttttttgtt agtggcgaca tttctattgg
     2581 ataatctgtg ataaaagtag gttgaataaa atggtcttca catttttctc caaaaatagc
     2641 atcaatcaat ttgcctttac ccattgtgtc gtcaatttct atatctaatt ctttgcaaat
     2701 ttgtcttaat tcatcttcag attttcctgc aatgtcgtag cctgtatatt ttttaatggc
     2761 atcatacatg ctaactctcg caaatggagt tttatagtcg attattctat ctcctaattg
     2821 tactttagta gttccatgca gtgctatagc aactctttcg accatttctt cagtaaattc
     2881 catcatccat ttatagtctt tataagccac gtaaatttct aatactgtaa attctggatt
     2941 atgggttctg tccattcctt cgttacggaa atcctttgca aattcataaa ctccatcaaa
     3001 accacctact ataagttttt tcaaataaag ttcattagca attcttaggt aaagaggaat
     3061 atttaaagca ttatgatgag taacaaaagg acgagcagct gctccgccgg gaattggctg
     3121 taatattggt gtttcaactt ctaaatatcc ttttgaatta aaaaactcac gcattgtgtt
     3181 tactattttt gttcgtttta caaacacatc tttcacatga ggatttataa tcaaatcaac
     3241 ataacgttga cgataacgca tttctaaatc tgttacggca tcgaaaacat gtccgtcttt
     3301 ttctttcact ataggaagtg gacgtaaaga cttagttaaa acagtaaatt ctttaacatg
     3361 aacagaaatt tcgcccattt gagttctaaa agcatagcct ttaacgccga taatatcgcc
     3421 aatgtcaagc aagcgtttaa acacaacatt atacattgtt ttatcttcac cagggcaaac
     3481 atcatcacgt ttaaaataaa gctgtattct tcctttacta tcttgtaatt cagcaaaaga
     3541 agcatttccc ataatacgac gagacataat tcttcctgca agagaaacag attttaaatt
     3601 ttctggcata ttttcatcag aaaatgtttc taaaatatct gttgagtaaa tatctacatg
     3661 atattctgct gctgggtatg ggtcaattcc taaattacga agttctttta acgattctct
     3721 tctctgaatt tctaagtcac ttaatccaat ataatccata taattttaaa attttagaat
     3781 ttttattgca aaaatacatt aaattttcga ttattagcaa atagatagaa ttaaattaag
     3841 aattaagaat tgtgtctcga cttcgctcga cacccgataa ttaagaatta cttccattaa
     3901 aagttaaaag ttgtgttgcc cttgcgggca aacctttgag agtcttgttt tgtactcacg
     3961 tacaacccat accaacacgc tccaacgccc atgatgctcg ctcggattta gtgaatttta
     4021 gaaattgtaa taaaaataaa agtttttaaa gttcaactca acaccataaa ctaaaaactc
     4081 aacactaaaa actaaaaact tttatttttc ttcttctgag ctttcttcta ctatataaag
     4141 aggacgtttc ctcacatcat tatttattct actaatatat tcgccaatta ttccgattgt
     4201 tattaattgc actcctccaa taaacgaaac agaaataatc actgaagtcc agcctctaag
     4261 tggtggagta tgcaaaagat aatgagatat taatgcgtaa acaataatac agaaagaaaa
     4321 tacagaaaca acaattccca tattgcttgc aaatttcaaa ggtcgtgaag aaaaacctgt
     4381 gatgccatca attgcaagtt tcattagttt actgaaagaa tagcctgttt ttccactaaa
     4441 gcgagggtct ctaacatatt ctacacatgt ttgtttgtag cctatccaag ctatttgacc
     4501 tctaagaaac ttatttcttt ctggcatttg tttaagatga ttaataatca ctctatctat
     4561 taaacggaaa tcgccagtat ctaaaggtat ttttacttct gtgagtttgt ccaatattct
     4621 ataaaataat ttatatgctg cttttttaaa tatattcact ccttttcttg cacttctttt
     4681 agcataaact accttataac cttctttgta ttttgcatac atttctttaa ttaattcagg
     4741 aggatcttga aggtctccat caataattgc tactgcatcg cctttggaat agtctaatcc
     4801 tgccgaaaca gctacttgat gtccaaaatt acgactaaaa ctaatatact gaactgaatt
     4861 atcctttttc gataaatctt taattattga cagagaatta tcttttgaaa aatcattaac
     4921 aaaaataatc tcatatgatg agcaaatttc tttcaaaact ttagttagtc tttcatacat
     4981 ttgaggaata tttccttctt cattgtaaat tgggacaaca acagatattt ccataataat
     5041 tacatgtttt gagtttctaa taatttagtt aagcgaacta ttttactttg aattttaaag
     5101 tgaagcactt cgtattcaaa tctttccata attttttcgt ctgtttcaat ttcatttatc
     5161 tgaacaagat ctccaagttt taaatcttca atgttttttc tctgattatg aatatcataa
     5221 tgattcattg cataaaagta aaacatagaa ctttggtcgt agtcgccatt tatatagttt
     5281 atagtatcat ttacaacaat atttcctttc aataaacttt tcattagttc agaacgagaa
     5341 taatatgcct gatacagagg atcttccttt ggacgataga tatgctttac aatacgcttg
     5401 tatgggatag agaaaactgc aatataagct acaataatta aaatataaat tttagaagtc
     5461 ttatatttta aatttataat ccaattgtga ataacataaa atgcagatcc aattactaaa
     5521 gcaaaaagag gcattaaagg aagtaggtac cagtgcaatt tagtcttggc aataagcaaa
     5581 aacacatact gcataattgc aatagacata gaaaaaatac taagtcgttt tacttgttta
     5641 tctttaacaa ataacgtaaa aataaaagcc aaaggaagta tataaaagaa ataagtaaaa
     5701 taatttgttt ttaaattgtt gatgtaataa catttacctc cagaattatt ttcaagaact
     5761 gtcataaaac gacctcccaa ttcattttca taaacagcct taaggtatcc gccataagat
     5821 agttctctcc ctatataata tcctccaaca aaaagaataa aaagtccaat tccaatatag
     5881 gttgttttgt cttttaagat aggcaataat tgttttctaa aaataaggta aattggaata
     5941 aaaggaagag gcatcaaagc ttgaattccc tttgttagag tggcaaaaat aagagaaaca
     6001 aagaaaagta tatagtattt tttttctttg gtttctgtat aaataaagaa caaaagaaga
     6061 taagcaatca taaaaaaaca aagcaaggca tcataatcgg cacttcttcc actatgataa
     6121 ataaaaaatc cccttgagca aattatcact gaagcagcat agaaacctaa gaatggtttt
     6181 tttataactc ctgtaagtaa aataataaca actcccatga gcaatattgc aagagcagat
     6241 ggcaatctta ctgcaacttc attagctcca aaaaccttga tactcaaagc ttgaatccaa
     6301 atagccaaag gaggttttac gctccaatga tctggcttat aatcgtaagt tacaacaata
     6361 ggatttttgg tttttgtcat ttcataagaa gaagcagcta ctctactttc gtcccaggtt
     6421 cttaaaggtt gagttcccaa aaatgcaaat aaaggatata agcttataag aacaaatatc
     6481 acaacaagta tccacgtaag ttttttgtta gagattttct tttccattca cttttgttct
     6541 aaatgaattt acaaaagtag taaaataata tttataacaa aatgtgtcaa cttttcttag
     6601 aacgcgacag gctgacgcgt ctattgcttc gcagagttcg caagctcact ctacttgcat
     6661 gggttagttt taaaccacaa aagacacaaa agagcacaaa agttaaaagc gatgcactga
     6721 acgtagttga attgtcgaaa gaatttcggt gtttcgactt cgctcaacac ccgatattgg
     6781 aatcgctcga cacccgatat tcaaataact ttatgaactt taaaaacttt taactaattg
     6841 ttttttaatt aataatattt tttactgctc taatataatt ttaaacaaca aagataaaaa
     6901 atttttcagt taacagtcag taaatttgat taaaattaat aaaaaatgcc cccgataaag
     6961 gacgagggca accttaatgt tttcacaacg gaataatcct tattgtgaat tggtttcaaa
     7021 ttttgtacga caaagataat gctttttttt gaaaataaaa caaatattaa taaatttgta
     7081 aaataatttt aatatttgtt aatatcatga aaaaaattct cggattagac ttaggaacaa
     7141 atagtattgg ttgggcgttg gttaatgaat cagaaacaga aaacgaacaa tcatctattg
     7201 taaaacttgg agttagagta aatcctctaa ctgttgatga aataagcaat tttgaaaaag
     7261 gaaaaagcat tactacaaat gcagaaagaa ctttaaagcg tggagcaaga cgtaatttgc
     7321 aacgttataa acttcgtaga gaaaatctaa tagaaattct tagagagaat aaaataatag
     7381 cagacaaaac tattctttcc gaagatggca aagatacgac tcaccaaact cttaaactgc
     7441 gagcaaaagc agcaaaagaa aaagtttctt tagaagaatt tgctaaaata ttgctgttga
     7501 ttaataaaaa acgtggctac aaaagtaatc gaaaagctca atcaagcgac gaaggacaac
     7561 ttattgacgg aatggctgtt gcaaaagaac tttatgaaaa agacattaca cctggagaat
     7621 tttcatataa tttgcttaaa aatggtaaaa ataatctacc tgacttctat cgctctgatt
     7681 tacaaatgga gtttgataga atatggaaat tccaacataa gttttatcca gaaattttga
     7741 ctgatgattt aaaaaacaat ttaaaaaaca aagccaaaaa agacacttgg gctatttgtc
     7801 aaaaaccttt taacatcgtt ggtattaaac gtaacactaa aggtgctgaa caaaaattag
     7861 aaaactataa atggcgaaaa gatgctctta caaaacaatt agaactcgaa gaattggcaa
     7921 ttgtcctaca agaaatcaat ggacaaataa atagttcaag taactatctt ggcgatatta
     7981 gcgaccgaag caaagaattg tatttcaaca accaaaccgt aggtgaatat caaatggcac
     8041 agttggtagc aaatcgacac caaagcctta aaaatcaagt tttttatcgc caagattatt
     8101 tagatgaatt tgaaaaaatt tgggaaacac aagcaattca tcacaaagag cttactcctg
     8161 aactaaaaaa agagattaga gatgttgtaa tattctatca acgtccacta aaatcacaaa
     8221 aaggcttact ttcattttgc gaatttgaaa gtaaagaaat tgaaatatta attaatggag
     8281 taaagaaaaa gaaaactaca ggactacgtg tttgcccaaa atcttcgcct ctttttcaag
     8341 aatttagaat ttggcaagta ttaaacaaca ttgaagtaaa aggcgttaca aagaaaaaca
     8401 aaaataatat agagccaact cttttcgaag ataacttcaa tgaaaaaaaa cttatccaga
     8461 aagtgcaacg tcctctagag caagaagaaa aagaattatt agcagaaaga ctcatgctct
     8521 gcgataaaat gactaaaaat caaatgctaa cagcactagg cttaaataat tacgacatga
     8581 acttcaaaga aataaaaggc aatacaactt ttgcaaaatt atttcaagcc tataaagaaa
     8641 ttgctgaaat agttggatat gacaaagata aaatcaaaaa cttagaagat ataaatatta
     8701 ttttcaaaga tttaggaata aagactgatt ttctttcttt taactctgaa ttagatagaa
     8761 aagagttgca acaacaatct ctttataaac tttggcattt aatatattct tacgaaagcg
     8821 acaattctgc aacaggaaac gaaagtctta taagaaaaat ttcggaacta tgtggaatgc
     8881 ctgaagatta tgctaaaatt ctggcaaata tagttttcga aagtgattat tctagccttt
     8941 ctgctaaagc tataggcaaa attcttccac atttaaaaga tggac
//
LOCUS       DAOPNX010000099         8725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_9026_length_8725_cov_7.749250, whole genome shotgun sequence.
ACCESSION   DAOPNX010000099 DAOPNX010000000
VERSION     DAOPNX010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8725
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9026_length_8725_cov_7.749250"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..473)
                     /gene="raiA"
                     /locus_tag="PKN53_09510"
     CDS             complement(177..473)
                     /gene="raiA"
                     /locus_tag="PKN53_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOD89224.1"
                     /translation="MDIKIQSVRFDADKKLIAYIENKVGKLSQVADNIIDAEVVLRLE
                     NSETKDNKVAEIKINVPYAKDIFAKRQKSTFEESVDLATVALRRQLKKYKEKLR"
     gene            complement(492..1379)
                     /locus_tag="PKN53_09515"
     CDS             complement(492..1379)
                     /locus_tag="PKN53_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOD89225.1"
                     /translation="MNINNFIKYIRYEKRLSEHTINSYETDLIQFRNFVFVETNDDEL
                     NPEYQKIRAWIASLSEQGLSAVSINRKVSSLKSYVKYLMKFGHISKNPMLKIVLPKAK
                     KRLPEFVQENQMNNLDNASLFSDDFFGIRDRFMIELFYNTGMRLSELINLKHIDIDVS
                     RGNIKILGKGNKERIVPFNTYINDMYKLYVSQKSDFGFSCEAKDFIFVTDKGEKMYPN
                     FVYRKIQHYLGLVSQLEKRSPHVLRHSFATHMLNNGADLNSIKELLGHTSLAATQVYT
                     HNTFSKIKTIYKQAHPRAQ"
     gene            complement(1458..1655)
                     /gene="rpsU"
                     /locus_tag="PKN53_09520"
     CDS             complement(1458..1655)
                     /gene="rpsU"
                     /locus_tag="PKN53_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOD89226.1"
                     /translation="MIIVPVKDGENIDRALKRFKRKYEKTGVVKELRRRQAFEKPSVT
                     KRQQMIRARYVQRLLTMQENN"
     gene            1852..3240
                     /gene="glmM"
                     /locus_tag="PKN53_09525"
     CDS             1852..3240
                     /gene="glmM"
                     /locus_tag="PKN53_09525"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOD89227.1"
                     /translation="MTLIKSISGIRGTIGGFAGENLTPIDIVKFITAYSLWQKETNPK
                     AKTIVVGRDARPSGKMVENIVVSVFNSLGFDVINIGLATTPTTEFAVINEKADGGIII
                     TASHNPKNWNALKLLNNKGEFLSASEGQRILELAETANAEFAQVDDLGKTTKLRNYEN
                     LHIDSILKLKLVNKELIKATDFTVAIDCVNSVGGFAIPKLLKSLGVKNIIELYCTPNG
                     QFPHNPEPLPEHLTEISNLIKEKNADVGFVVDPDVDRLAIICEDGSMFGEEYTLVAVA
                     DYVLSKTPGNTVSNLSSTRALRDVSEKYGVEYNSAAVGEVNVVAKMKETNAVIGGEGN
                     GGVIYPESHYGRDALVGIALFLSFLAEKQLKVSELKKTYPNYFISKNKIELTKDINLK
                     NIFEKLQEKYKDNPLHTIDGLKIEFPETKEWVHLRSSNTEPIIRIYCESLNQKNAEAL
                     AKKFIEEIKNLM"
     gene            3308..5116
                     /gene="uvrC"
                     /locus_tag="PKN53_09530"
     CDS             3308..5116
                     /gene="uvrC"
                     /locus_tag="PKN53_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOD89228.1"
                     /translation="MISKDDLDIKVKALPQNPGVYKYLDKAGNIIYVGKAKNLKKRVS
                     SYFAKNHQSFKTNVLVKRIKDIEYIVTETENDALLLENVLIKSLQPRYNVMLKDDKTY
                     PWICIKNETFPRVFYTRQKTKGNEYFGPFTSVYTIKTLLNLITQLYPIRNCNLNLSKE
                     KVKEGKYKVCLEYHIGNCKAPCIGEIDEKTYLQLISDIRKILKGNLHQVQTYFTKEMK
                     NHADKLEFEKAAEIKSKLSIIENYKSKSTIVNTNISDTEAFGFAKDINSAYVSYIRIQ
                     SGTIISAHSVEIRKKIDESDEDMLLFAVLELRESLNSDAKTTLLPFEVDFEVENTKIE
                     VPKRGDKLSVIELANRNAKFFMLEKHKQIENKNPEKHIQRKLETLKKDLNLSELPRHI
                     ECFDNSNIQGTNSVAACVVFKNARPSKADYRHYNIKTVEGADDYASMSEIVYRRYKRL
                     IDEKKELPQLIIIDGGKGQLNAALKSLKKLNLENISIISIAEKLEEIYFPNDSVPLFL
                     DKNSESLKLIQHARDEAHRFGLSFHRNKRSANFIVSELENIAGIGDKTIDVLLTKYKS
                     VNQIKKAKIEDLETSIGKAKAKLLIDYFKIKQNSNE"
     gene            5109..5543
                     /locus_tag="PKN53_09535"
     CDS             5109..5543
                     /locus_tag="PKN53_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89229.1"
                     /translation="MNKIKYFFHLLSYLQYPLLIVGLFFIVKPFFKGFDYLSSNSEYV
                     FQNYNYALIFLGLTLGFASLQDSTKTTLKYEKNIWRNPKKAKRIMIFTIATMIIFLFA
                     GVFGFIMKDSFVKDFAYGSIVLAVGLLGYLKLQMEMYENHKP"
     gene            5649..6386
                     /locus_tag="PKN53_09540"
     CDS             5649..6386
                     /locus_tag="PKN53_09540"
                     /inference="COORDINATES: protein motif:HMM:NF016915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89230.1"
                     /translation="MKRLTNIFLIIVLISFSFEVIASHKIYILHGYASSKSIMNRIKK
                     DLKKSGFLVENYDYPALYVDLDSLGKKLYLDVLEDNYDSVSFVTHSMGGLVVRNMVKY
                     SAADLNFPKIYRIVMIAPPNQGADIADFFKKNKCNRKILGPNVEKMRTDSMSYANLLP
                     VPINTEIGIIVGKSKHKAGFNWFIGKKNDGLLIPERVHLGNEKDIATFNKSHIGVVKR
                     KKPREAVVKFMKFGKFNVENNTDSISP"
     gene            complement(6373..6750)
                     /locus_tag="PKN53_09545"
     CDS             complement(6373..6750)
                     /locus_tag="PKN53_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOD89231.1"
                     /translation="MEFNLKIGTQGNQEQIVKFEDTAANYGSGLVEVFATPAMIALME
                     NTCYKSVMEFLPEGYGTVGTKVDIVHIKATAVGKKVSCKSELIEIDRKRLVFSVKAFD
                     EDGEIGSGIHERFIINVEKFMGK"
     gene            complement(6789..7418)
                     /locus_tag="PKN53_09550"
     CDS             complement(6789..7418)
                     /locus_tag="PKN53_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOD89232.1"
                     /translation="MIKIFYKNKIVILTEKEFDNIYSLKIDEFKKKALKKQIIKFLDD
                     ENLDKINIYGKEPEKMLRKLRKIFVFVLAAGGLVKNSNDEILYIRRLGYLDLPKGKVE
                     ADETTEQAAIREVSEECGILEKDLKLSFQLPNTYHIYSQKGEYALKETQWFAMQFEEN
                     YKLKPQKKEKIVEVGWENFNELESKFKIESYPSLINFLDEIVAQNKLKA"
     gene            7425..8066
                     /gene="pyrE"
                     /locus_tag="PKN53_09555"
     CDS             7425..8066
                     /gene="pyrE"
                     /locus_tag="PKN53_09555"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOD89233.1"
                     /translation="MNQIDRQIANKLLQIKAIKLQPTKHFTWASGWFSPIYCDNRKTL
                     SFPEVRTFIKEAFVKTISENFKDFDVIAGVATGAIAQGVLVAEAMNKPFVYVRSQAKE
                     HGMGNQIEGQLDENKKVVVIEDLISTGGSSLSAVRAIREANCEVVGMIAIFTYNFQKA
                     IDNFEKENVKLITLSNYDVLIEEAIAMGYVSNSDLNLLKEWRLDPENWKKNQI"
     gene            8068..8478
                     /locus_tag="PKN53_09560"
     CDS             8068..8478
                     /locus_tag="PKN53_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HOD89234.1"
                     /translation="MKELEIISKTGKINNSAERIFNFFSDFRNISKLIPPEAGVENWQ
                     AEKDWCSFVVKGQNIQLNIVEREEFKTIKISGDDNSPYIFNFWMQLVQLSAYDTAVRL
                     VVRAQLSFFMRQAVKKPMQKGLDQIIDYMKMIPY"
BASE COUNT         3144 a         1295 c         1240 g         3046 t
ORIGIN      
        1 accgccgacc ggctgattac aaatcagctg ctctaccaac tgagctacat cggccaaaat
       61 ataaattaat tcatactttt taaagaacat ctttttactt acgaaaaagg tctgcaaatt
      121 tatgaattat ttatttcttt ccaaaatttt ttaattattt ttttaattat ttttaattat
      181 cttaattttt ctttatactt tttcaactgg cgacgtaacg caaccgttgc taaatctaca
      241 gattcttcaa aagtactttt ttgacgtttt gcaaaaatat ctttagcata tgggacattt
      301 attttgattt ctgccacttt attgtccttt gtttccgaat tttctaagcg taaaacgact
      361 tctgcatcga taatattgtc tgcaacttga cttaatttgc caactttgtt ctcaatataa
      421 gcgattaatt ttttatccgc atcaaaacga actgattgaa ttttaatatc catattgtag
      481 tcctttcttt tttattgtgc tctcggatga gcttgtttat aaatagtttt aatttttgaa
      541 aatgtattat gtgtataaac ttgtgttgcc gctaaacttg tatgtcctaa taattctttt
      601 attgaattca aatctgcacc attgttcaac atgtgagtag caaaactatg tcgcaacacg
      661 tgaggagaac gtttctctaa ttgacttact aatcctaaat aatgttgaat ttttctgtaa
      721 acgaaatttg gatacatttt ttcgccttta tcggtaacaa atataaaatc ttttgcttca
      781 catgaaaaac caaaatcact tttttgagaa acgtacaatt tatacatatc attaatatag
      841 gtattaaatg gcactattct ttccttattt ccttttccta aaatttttat attgccccta
      901 gaaacatcaa tatctatatg ctttaaattt atcaattctg aaagacgcat tccagtatta
      961 taaaaaagct caatcataaa cctatctcta ataccaaaaa agtcatctga aaacaaacta
     1021 gcattatcta aattgttcat ttgattttct tgaacaaact ctggcaatct ttttttagcc
     1081 tttggtaaca ctatttttag catagggttt ttagaaatat gtccaaattt cattaaatat
     1141 ttcacgtaag attttaagct cgacactttc ctattaatgc taactgctga taatccttgt
     1201 tctgacaaag atgctatcca agctctaatt ttttgatatt cgggatttaa ttcatcatca
     1261 tttgtttcta caaatacaaa attacgaaac tgaattaaat ctgtttcgta agaatttatt
     1321 gtatgttcag aaagccgttt ctcgtaacgg atatatttaa tgaaattatt aatattcata
     1381 ttaatttaat catcactacc aagaaatcta ttaattaata cgaatgtttt aattaaaagt
     1441 tacattaaat gtgaatttta attattttct tgcattgtta acaaacgttg aacatagcgt
     1501 gcgcgaatca tttgttgacg ctttgttact gaaggttttt caaaagcttg acgtcttctt
     1561 aattctttta caacgcctgt tttttcatat tttcttttaa aacgtttcaa ggctctgtca
     1621 atgttttcgc cgtcttttac tggtacaata atcattgtta ttatttttta tttaaactta
     1681 tttttatttc ttttaaattt tggactgcaa aaatatacaa tttatattat ttaacaaact
     1741 ttttcaacta atatttttgt aattttcata ttaggacttt ttgcattaaa aatcattttt
     1801 ttgcttcatt cttatcataa ctaatgttat ttttgcataa aattaaaaag aatgacttta
     1861 ataaaatcta tttctggaat tagaggtact ataggaggtt tcgctggcga aaacttaact
     1921 ccaattgata ttgtaaaatt tataactgcc tattcgttgt ggcaaaaaga aacaaaccca
     1981 aaagctaaaa caatagttgt tggtcgtgat gctcgtcctt ctggcaaaat ggttgaaaat
     2041 attgtagttt cagtttttaa ttctttgggt tttgatgtta ttaatattgg cttagccaca
     2101 actcccacaa cagaatttgc tgtaataaac gaaaaagctg atggcggaat aattataact
     2161 gccagccaca atcctaaaaa ttggaacgca cttaaacttt tgaataataa aggcgagttt
     2221 ttaagtgcta gcgaaggaca aagaatttta gaacttgcag aaactgcaaa tgcggaattt
     2281 gctcaagttg atgatttagg aaaaactaca aaacttagaa attacgaaaa tctacatatt
     2341 gattctattc taaagctaaa attagtaaac aaagaattaa taaaagctac tgattttaca
     2401 gttgctatag attgtgtaaa ctctgttgga ggctttgcta ttccaaaatt attaaaaagt
     2461 ttaggtgtaa aaaatattat tgagctttat tgtaccccaa atggtcagtt tccacataat
     2521 ccagagccgt taccagaaca tcttacagaa attagcaatc ttataaaaga aaaaaatgca
     2581 gatgttggtt ttgttgttga ccctgacgtg gacagacttg caataatttg tgaagatggt
     2641 tctatgtttg gcgaagaata tactttagta gctgttgcag actatgtttt atcaaaaact
     2701 cctggcaata ctgtgtcaaa tctttcatca accagagctt tgagagatgt ttctgaaaaa
     2761 tacggagttg aatacaattc tgctgctgta ggcgaagtaa atgttgtagc aaaaatgaaa
     2821 gaaacaaatg ctgtaattgg tggcgaaggg aatggtggtg taatctatcc agaatctcac
     2881 tacggaagag atgccttggt aggaattgct ttgtttttaa gtttcttagc tgaaaaacaa
     2941 cttaaagttt ctgaattaaa aaaaacatac ccaaattatt ttatttcaaa aaacaaaatt
     3001 gaacttacaa aagatattaa tttaaagaat atttttgaaa aacttcaaga aaaatataaa
     3061 gacaaccctc tgcacacaat agatggctta aaaatcgaat ttccagaaac caaagaatgg
     3121 gtgcatttac gctcatcaaa tacagaacct attattagaa tttattgcga aagtttaaac
     3181 caaaaaaatg ctgaagcttt agcaaaaaaa tttatcgaag aaataaaaaa tttaatgtaa
     3241 actatacaaa acaaattgat ataaacacaa atacagattt gttattttta ttttgtaatt
     3301 ttacatcatg atttctaaag atgatttaga cattaaagta aaggctttgc cacaaaatcc
     3361 tggcgtttac aaatatcttg ataaagctgg gaatattatt tatgtaggaa aagctaaaaa
     3421 cttaaaaaaa cgagtgtctt catattttgc aaaaaatcac caatcattca aaaccaatgt
     3481 tttagtaaaa agaataaaag acattgaata cattgttaca gaaactgaaa acgatgctct
     3541 tcttttagaa aatgttttga taaaatctct tcaaccacga tataatgtaa tgttgaaaga
     3601 tgataaaaca tatccgtgga tttgcattaa aaacgaaacc tttccacgag tattttacac
     3661 tcgacaaaaa acaaaaggta atgaatattt tggaccattt acttcggttt acacaataaa
     3721 aactttgtta aatttaatta ctcagctcta ccctattcgt aactgtaatt tgaatcttag
     3781 taaagaaaaa gtaaaagaag gaaaatataa agtttgttta gaatatcata ttggaaattg
     3841 caaggctcct tgcattggtg aaattgacga aaaaacttac ctacaattga tttcggatat
     3901 tagaaaaatt ttaaagggca atttgcacca agttcaaaca tactttacaa aagaaatgaa
     3961 aaatcatgca gataaacttg agtttgaaaa agcagctgaa ataaaatcaa aattaagtat
     4021 tatcgaaaac tacaaaagca aatcaactat tgtaaacaca aatatttcag ataccgaagc
     4081 ttttggtttt gcaaaagata ttaattctgc ttatgtatca tatatacgaa tacaatctgg
     4141 cacaataatt tcggctcatt ctgttgaaat ccgcaaaaaa attgatgaat ctgatgaaga
     4201 tatgcttttg tttgctgttt tagaacttcg cgaaagttta aactctgatg caaaaaccac
     4261 tttactacca tttgaggttg attttgaggt agaaaacact aaaatagaag ttccaaagcg
     4321 tggcgacaag cttagtgtaa tagaacttgc caatagaaat gcaaaatttt tcatgttgga
     4381 aaaacacaaa caaatcgaaa ataaaaatcc cgaaaagcat attcaaagaa aattagaaac
     4441 tttaaaaaaa gatttgaatt tgtcggaact gccacgccat attgaatgtt ttgacaattc
     4501 aaatattcaa ggaacaaatt ctgttgctgc ttgcgtagtt tttaaaaatg cacgcccttc
     4561 aaaagcagac tatcgccatt ataatataaa aactgttgaa ggtgctgatg attatgcttc
     4621 tatgagcgaa attgtttatc gccgctataa aagattgatt gacgaaaaaa aagaactacc
     4681 tcaactaatt attattgatg gcggaaaagg tcaactaaat gcagctttaa aaagtttaaa
     4741 aaaactaaat ttagaaaaca taagcattat aagcattgcc gaaaagttag aagaaattta
     4801 ttttcctaac gattctgtac ctttgtttct tgataaaaat tcagaatctt taaaattaat
     4861 tcagcacgct agagacgaag cccacagatt tggattaagt tttcacagaa ataaaagatc
     4921 tgctaatttt atagtgtctg aactcgaaaa tattgcggga attggagata aaacaataga
     4981 tgtattatta acaaaataca aaagcgtaaa ccaaattaaa aaagctaaaa tcgaagactt
     5041 agaaacatct attggcaaag ctaaagcaaa acttttaatt gattatttta aaattaaaca
     5101 aaattcaaat gaataagata aaatattttt ttcacctttt aagctatttg caatatccac
     5161 tattgattgt aggattgttt tttattgtaa aacccttctt taaaggattt gattacttgt
     5221 cttcaaattc tgaatatgta tttcagaatt ataattatgc attaattttt ttaggattaa
     5281 cactaggctt tgcttctctt caagattcaa caaaaactac tcttaaatac gagaaaaata
     5341 tttggcgaaa tccaaaaaaa gcaaaacgaa tcatgatttt cacaattgct acaatgatta
     5401 tttttctttt tgcaggcgtg tttggattta taatgaaaga cagttttgtc aaggattttg
     5461 cttatggttc tattgttttg gcagttgggc ttcttggata tttaaaatta caaatggaaa
     5521 tgtatgaaaa tcataaacca taaaaaaaag tgtttgctta aattaggaat agtaattaaa
     5581 tttgcaatca tataaaatat ccaaacccaa gttttagaaa gcataattta ttattgatta
     5641 ttttaaaaat gaaaagacta actaatattt ttttgattat tgtattaata agtttttcgt
     5701 ttgaagttat tgcttcacat aaaatttata ttttacacgg atatgcaagc agcaaatcaa
     5761 taatgaatag aataaaaaaa gatttaaaaa aatctggttt tttagtagaa aactatgact
     5821 atcctgctct ttatgttgac ttggacagtt taggtaaaaa attatactta gatgttttag
     5881 aagataatta tgactctgtt tcctttgtaa ctcactccat gggtggatta gttgtgagga
     5941 atatggtaaa atattctgct gcagacttaa attttccaaa aatttataga attgtaatga
     6001 tagcaccacc aaatcaaggt gcggacattg ctgatttttt caaaaaaaat aaatgtaata
     6061 gaaaaatatt aggtcctaat gttgaaaaaa tgagaacaga ctctatgtca tatgcaaatc
     6121 tactgcctgt tccaataaac acagaaattg gaataattgt tggaaaatca aaacataaag
     6181 ctggatttaa ttggtttatt ggcaaaaaaa atgatggact tttaattcca gaaagagttc
     6241 atttaggaaa tgaaaaagat attgctactt tcaataaaag tcatattgga gttgtaaaaa
     6301 ggaaaaagcc aagagaagca gttgttaaat ttatgaagtt tggaaaattt aatgttgaga
     6361 acaatacaga ttctatttcc ccataaattt ttctacattt ataataaatc tttcgtgaat
     6421 accacttcct atttcgccat cttcatcaaa agctttaacc gaaaaaacta atcgttttct
     6481 atcaatttct attagttctg atttgcaaga aacttttttc ccaactgccg ttgcttttat
     6541 gtgaacaata tccactttag ttcccacagt tccataacct tctggcaaaa attccataac
     6601 agatttatag caagtatttt ccattaaagc aatcatagca ggagtagcaa aaacttcaac
     6661 taatccacta ccatagtttg ctgccgtatc ttcaaacttc actatctgct cctgattacc
     6721 ttgtgttcct atttttaaat taaactccat aaaatgacta ttttgtttgt acaaaaataa
     6781 aaaataattt atgctttaag tttgttttga gcaacaatct catctaaaaa attaatcaaa
     6841 cttggataag attctatttt aaatttgctt tctaattcat taaaattttc ccaaccaact
     6901 tctacaattt tctctttttt ttgaggtttt agtttataat tttcctcaaa ctgcatagca
     6961 aaccactgtg tttcttttaa tgcatattct cctttttgcg aataaatgtg ataagtattt
     7021 ggtaattgaa aactaagttt taaatctttt tctaaaatgc cacactcttc tgaaacttcg
     7081 cgaatagcag cttgttctgt agtttcatct gcttcaactt ttccttttgg caaatccaaa
     7141 tatcccaatc ttcgaatata taaaatttcg tcatttgaat tttttaccaa acctcctgca
     7201 gctaaaacaa aaacaaaaat ttttctcaat tttctcaaca ttttttctgg ttctttccca
     7261 tatatattga ttttatctaa attttcatca tctaaaaatt ttataatttg tttttttagt
     7321 gcctttttct taaattcatc tatttttaat gaataaatgt tgtcaaactc tttttctgta
     7381 agaattacta tcttattttt gtaaaaaatt ttaatcataa ttttatgaat caaattgata
     7441 gacaaattgc caataaactt ttgcaaatta aagcaataaa attacaacct acaaaacatt
     7501 ttacatgggc atcaggttgg ttttctccaa tttattgcga caaccgtaaa accttgtctt
     7561 ttccagaagt tagaactttt ataaaagaag cttttgtaaa aacaattagt gaaaacttta
     7621 aagattttga tgtaattgca ggcgtagcaa caggagctat tgctcaagga gtcttagttg
     7681 cagaagcaat gaacaaacct tttgtttatg ttcgttcaca agcgaaagaa cacggaatgg
     7741 gaaatcaaat tgaaggacaa cttgacgaaa acaaaaaagt tgtagtaata gaagatttaa
     7801 tttctactgg tggaagtagt ttgagtgcag taagagcaat tcgtgaagca aattgcgaag
     7861 ttgttggaat gattgctata tttacttaca attttcaaaa agcaattgat aattttgaaa
     7921 aagaaaatgt aaaattaatt acactaagta attacgatgt tttaattgaa gaagctattg
     7981 caatgggtta tgtttcaaat tctgacttaa atttattaaa agagtggcga cttgaccctg
     8041 aaaattggaa aaaaaatcaa atttaaaatg aaagagttag aaattattag taaaacaggt
     8101 aagattaata attctgctga aagaattttt aatttttttt ctgattttag aaatatttca
     8161 aaattaattc caccagaagc tggagttgag aattggcaag cagagaaaga ttggtgttct
     8221 tttgttgtta aaggacaaaa catacaacta aatattgttg aaagagaaga gtttaaaacg
     8281 ataaaaatct ctggagacga caattcgccc tatatattta atttttggat gcaattagta
     8341 caactctcgg catacgatac tgctgtaaga cttgttgtgc gagcacaact aagtttcttt
     8401 atgcgacaag ctgtaaaaaa acccatgcaa aaaggattag accaaatcat tgattatatg
     8461 aagatgatac cttactaagg tattcaaatt ttctattatt ttcttcttat acataaaaag
     8521 gcggtgtctc ataagtgtca aactgcttta cttcaacttc gttcagcaac cacgttgctt
     8581 tcaatattcg gactcagtca cgtacacttc ggctttgctc agtgcaacgc ttcatgtacg
     8641 ctccttcgtc ctcaatttca gcgccttgca ttttaaccct aactccgtcc ttttaggtag
     8701 tctatattta ttttttcaaa taatt
//
LOCUS       DAOPNX010000100         8548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_9253_length_8548_cov_5.689274, whole genome shotgun sequence.
ACCESSION   DAOPNX010000100 DAOPNX010000000
VERSION     DAOPNX010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8548
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9253_length_8548_cov_5.689274"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="PKN53_09565"
     CDS             complement(<1..1537)
                     /locus_tag="PKN53_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOD89235.1"
                     /translation="MKNFFFLTFMVCGLLLANGTFGQTPVINITGLESEYTVSQDIAI
                     TATISSNGAIDSLCSMGYKIYKDDAVTPIENISDFGSLNYKFRLQDEEFYNGTITQGS
                     GMIEIQVGEDTFGAFTLGIFDTYCIDRNRPINVTANFNETGTYKFNLTLYYCENDGVA
                     TGTSFTADATCGGGEHNDATAQTCESPTIFATKNFEINVVAADEITIISQSESTSICE
                     GSSTTISVDATANNSLTINYKWYKDGTEFGNTTNAITVDAEGDYYCELTAGTASATTE
                     TISITYHPTPSISLNPTTPMCSGEAVVLNPGEFASYEWSDNSTEPTLSVTEAGNYSVT
                     VTDEFGCTATASTVVTAGGEMPEEFSFTDTTICYGITHALEGPFNETYTYLWNTEDTT
                     YSIIVEEAGTYSLTVTNTDGCKREGSVNIDFAYTVNAQIAGGDDEVHSCSDAPNVRLG
                     PIPEGSSWLWSNESTSRTIRVTTPGIYWVVATDTATGCIGTDTVELFIHQIPEVNLGD
                     DQAY"
     gene            1695..3350
                     /locus_tag="PKN53_09570"
     CDS             1695..3350
                     /locus_tag="PKN53_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD89236.1"
                     /translation="MVEKTKTLLRDKASARWTALILLSFAMFCAYIFMDILSPIKDLL
                     ESTRGWDSTAFGTYAGSETFLNVFILFLIFAGIILDKMGVRFTAILSGIVMLIGATIN
                     WYAVTEAFMGSSLETWFTENLNYIPIFDELGVSPFYRGMPASAKFSAVGFMIFGCGVE
                     MAGITVSRGIVKWFRGKEMALAMGSEMALARLGVATCMIFSPVIANAFGRPDVSRSVA
                     FGVVLLMIALIAFIAYAFMDKKLDAQTGEAEEKDDPFRVRDLGKIFSSLGFWLVALLC
                     VLYYSAIFPFQKYAVNMLQCNLDFVKPEEGSFWASNLVAVVQYIVMLVVAATAFASNF
                     VKKRQVQFSLLAVSIISLLVFCYMAYQRQSAESIFAVFPLLAVGITPILGKYVDNKGK
                     AASMLMLGSTLLIVCHLTFAFILPEFKGNNIGGVIVAFLTILVLGASFSLVPAALWPS
                     VPKLVDSKIIGSAYALIFLIQNIGLWLFPLLIGKILDWSNPEIAQAVADGTMTSTEAS
                     SYDYTNPLIMLAMLGVAALILGFVLKSVDKKRGYGLELPNIQS"
     gene            3439..4299
                     /locus_tag="PKN53_09575"
     CDS             3439..4299
                     /locus_tag="PKN53_09575"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HOD89237.1"
                     /translation="MKKISFILLLFVSSSLFSQSLLWKISGKKIKNPSYIYGTIHIQD
                     KRAFSFDESVLEAFESSDAFAMEILMDEIPMQEMMDAMLMKNNTLDKLYTKEEYRVLD
                     SIVKVKTGAGVLLYNKMKPFFLSSQLMQLEMPKDMDLALDLFFLTEARKAGKPCYGVE
                     KFMDQISAIDAISLEEQADMLYKSLTDTAENDTMSKLNEMLETYMNFDLDKMLELSTD
                     STMPADFNENFLIKRNVVMVKNFLKISKKQSLFCTVGAAHLAGEDGVLELLRKKGYTV
                     EPVFFKWTEN"
     gene            complement(4365..7886)
                     /gene="nifJ"
                     /locus_tag="PKN53_09580"
     CDS             complement(4365..7886)
                     /gene="nifJ"
                     /locus_tag="PKN53_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOD89238.1"
                     /translation="MSKEKKFITCDGNHAASHVAYMFSDMACIYPITPSSNMAENVDE
                     WAAHGRKNIFGQTVKVEEMQSEAGAAGAVHGALQAGALTTTYTASQGLLLMIPNMYKM
                     SGELLPAVFHVSARSLAAQALSIFGDHSDVMSTRQTGFAMLATSSVQQIMDLAPIAHL
                     AAIKSRVPFMHFFDGFRTSHEIQKVEYFDNEEMAALLDWEALDNFRKSAQNPDNPVTK
                     GTAQNPDIYFQSREAANKFYTNIPDLVEEYMQEITKLTGREYHPFTYHGAADAENIIV
                     AMGSITDLLIETVDYLNNSGKKVGVITVHLYRPFSEKYFMKVLPKTVKRIAVLDRTKE
                     IGANGEPLFLDVKEMFYNKENRPMIIGGRYGLSSKDTTPAMMISVFENLASDKPKDRF
                     TVGIVDDVTNLSLPLKENISILDKGTFEAKFYGLGSDGTVGANKNSIKIIGDSTDKYC
                     QAYFAYDSKKSGGFTASHLRFGDKEIRAPYLVTTPDFVACHVPKYVELYDVLEGIKPN
                     GSFLLNSLHDAEETKKNLPDHMKKVLAEKNIKFYIINATQIAEEIGLGNRTNTILQSA
                     FFKIANVIPYEKAVEQMKKMIDISYGLKGEKIVQMNYKAVDAGASVVQVDVPAEWANI
                     KTEGFKFKRDREVPEFVSKVADVINSQKGDTLPVSTFNGRENGSFPAGLTKYEKRGVA
                     VNVPEWIPENCIQCNQCSYICPHAAIRPFLLTDEEVKKAPAGTKTIQGAGPTKEYKFK
                     MQVSVLDCTGCGNCADICPAKTKALVMKPLETQMDEVDRWYYMDETVGYKENVVDKHS
                     NVKNSQFAQPLFEFSGACAGCGETPYIKLITQLFGERMIIANATGCSSIYGGSAPSTP
                     YCKNYNSGKGPAWANSLFEDNAEYGFGMASAIEKMREGIAMRLNEAKWTGEAAEAAKD
                     WLANMDNAEASEKASERLVKAIEKLNDPIAKEVMNKKQYLVKKSVWIFGGDGWAYDIG
                     YGGLDHVIASGKDVNILVMDTEVYSNTGGQASKSTPTAAIAKFAASGMKTRKKDLGAM
                     AMTYGYVYVAQVAVGANHAQYYKTIKEAEAYPGPSLIIAYAPCINHGLKAGMGKTQDE
                     ERRAVECGYWVNWRYNPQLEAEGKNPFQLDSKEPDWSKFQTFLDGEVRYNSLKKLFPE
                     TAEALLQEAERNAKWRYNFYKRLAEQK"
BASE COUNT         2627 a         1366 c         1445 g         3110 t
ORIGIN      
        1 agtatgcttg atcatcacca agattaactt cagggatttg gtgtataaat agttcaacgg
       61 tatcagttcc aatacatcct gtagctgtat ctgttgcaac cacccaatat atgccaggtg
      121 tagtaaccct tatagttctt gaggttgatt cgttactcca tagccaactt gagccttcag
      181 gtataggacc aagtcttaca tttggtgcat ctgaacaact atgtacttca tcgtcgcctc
      241 ctgcaatttg tgcattaacg gtatatgcaa aatcaatatt tacagaacct tcgcgtttac
      301 agccatcagt attagttaca gttaaagaat aagttccggc ttcttctaca attatggagt
      361 atgtagtatc ttcagtgttc cataaatatg tataagtttc attaaaaggt ccttctaatg
      421 catgtgttat accataacaa atagtagtat ctgtaaaaga aaattcttca ggcatttcac
      481 ctcctgctgt aactactgtg cttgcagttg cagtacagcc aaattcgtcg gttactgtaa
      541 cgctatagtt gccagcttct gttacgctta aagttggctc tgtgctatta tcgctccatt
      601 cataagacgc aaactctcca ggatttaaaa ccacagcctc tccagaacac ataggagttg
      661 ttgggtttaa agaaattgat ggtgttggat gatatgtaat gcttattgtc tcggtcgttg
      721 cagaagcagt acctgcggtt agttcacagt aataatcgcc ttctgcatca acggttattg
      781 cattagtagt attaccaaat tctgttccat ctttgtacca tttataattt attgttagtg
      841 aattgtttgc tgtagcatca acagaaatag ttgtacttga gccttcacaa atgcttgttg
      901 attcagattg gctaatgatt gtaatttcgt ctgctgcaac aacgtttatt tcaaaatttt
      961 tagtagcaaa aattgtaggg ctttcgcaag tttgcgcagt tgcatcatta tgttctccac
     1021 caccgcaagt agcatctgct gtaaaacttg tgccagtcgc aactccatcg ttttcacaat
     1081 aatataaagt gagattaaat ttgtaagttc cagtttcatt aaaattagct gttacattaa
     1141 taggtctatt tctgtctata caataagtgt caaatattcc taaagtaaaa gcaccaaaag
     1201 tatcttctcc tacttgaatt tctatcattc ctgatccttg cgtaattgtg ccattataaa
     1261 attcttcatc ttgaagtctg aatttgtaat ttaaacttcc aaaatcacta atgttctcaa
     1321 tgggtgtaac agcatcatct ttataaattt tatatcccat actacaaagt gagtcaatag
     1381 ctccatttga actaatagta gcagttattg ctatgtcttg tgaaaccgta tattcacttt
     1441 caagacctgt gatattaatt acaggtgttt gtccaaaagt gccgtttgct aataataatc
     1501 cgcaaaccat aaatgttaag aagaaaaaat ttttcatatt aaattaatta ataaataata
     1561 gtttataatt cttttaaaag gcaaaggtaa taataatttt ttgatatgaa aaaatattct
     1621 ttgttaaaaa atcacttttt tatttaaaaa ataatacatt tgcaaaaaat aaataaaatc
     1681 taaaaattac tattatggtt gaaaagacaa aaactttatt gcgagacaaa gcttctgctc
     1741 gatggacagc tttgattttg ctatcatttg cgatgttttg tgcttatatc tttatggata
     1801 ttttatctcc aataaaagat ttgctagaat ctactcgcgg ttgggactct acagctttcg
     1861 gtacttatgc tggttctgag accttcttga atgttttcat tctattttta atttttgcag
     1921 gtataatttt ggataaaatg ggtgttaggt ttacagctat tttatcagga attgtaatgt
     1981 tgattggggc tactattaat tggtatgcag taacggaggc atttatgggt tcaagtttag
     2041 aaacttggtt tactgaaaat ttaaactata tacctatttt tgatgaatta ggagtttctc
     2101 cattttatcg cggtatgcct gcgtctgcaa aattttcagc agttggtttt atgatttttg
     2161 gttgtggtgt tgaaatggct ggcattactg tttctagagg tattgtaaaa tggtttagag
     2221 gaaaagaaat ggcattggca atgggttctg aaatggcatt agcgcgtttg ggtgttgcaa
     2281 cctgcatgat tttctcgcct gtaattgcaa atgcttttgg aagacctgac gtttctcgtt
     2341 ctgttgcttt cggcgtagta ttattaatga ttgctttgat tgcttttatt gcttatgctt
     2401 tcatggataa aaaattagat gcacaaacag gtgaagcaga agaaaaagac gatccattca
     2461 gagtacgtga tttaggtaag attttctcta gcctaggttt ctggttggta gctttattat
     2521 gtgttctata ttattcagct attttcccat tccaaaaata tgctgtaaat atgttgcaat
     2581 gtaatttaga ttttgtaaaa cctgaagaag gatctttctg ggcttctaat cttgttgcag
     2641 ttgtacaata tatagttatg cttgtggtgg cagctactgc tttcgctagt aattttgtaa
     2701 aaaagagaca agtacaattt tcattactag cagtatcaat aatttcgctg ttagtattct
     2761 gctatatggc ttatcaacgt caatccgcag agtctatttt cgcagtattc ccattattag
     2821 ctgttggtat tacaccaatt ttaggaaaat atgttgataa taaaggtaag gcagcttcaa
     2881 tgttaatgtt gggctcaact ttactaattg tttgccattt aacttttgca tttattttgc
     2941 cagaatttaa aggtaataat attggaggtg taatagttgc attcttaaca attttagtac
     3001 ttggagcatc attctctttg gtaccagctg ctctatggcc aagtgttcca aaacttgttg
     3061 atagtaagat tattggttcg gcttatgctt tgattttttt aattcaaaat ataggtttgt
     3121 ggttgttccc attattaata ggtaagatat tagattggtc taatccagaa attgcacaag
     3181 ctgttgcaga tggaactatg acttctactg aagcgtcttc ttatgattat acaaatcccc
     3241 taattatgtt agcaatgctt ggtgttgcgg ctcttatttt agggtttgta cttaaatcag
     3301 ttgataagaa aagaggttat ggacttgaat tgccaaatat tcaatcataa catattttta
     3361 taagtttgat aaaattttaa aatatcctca aaatgtttta tttttgagga tattttttat
     3421 taataaattt aatgtattat gaagaaaatc agttttattt tattgttatt tgtaagttcg
     3481 tcattgtttt ctcagtcttt gttatggaaa ataagcggta agaaaattaa aaatccgtcc
     3541 tatatttatg gcacaattca tattcaggat aaacgagctt ttagttttga cgagtctgtt
     3601 ttagaagctt ttgaaagtag cgatgctttt gctatggaaa ttcttatgga cgaaattcct
     3661 atgcaagaaa tgatggatgc aatgttgatg aaaaacaaca cacttgataa attatataca
     3721 aaagaagaat atagagtttt agattcaata gttaaggtta agactggagc aggagtatta
     3781 ctatataata aaatgaagcc ttttttcttg tctagtcagt taatgcaatt ggaaatgcca
     3841 aaagatatgg acttggcttt agatttattt ttcttgacag aggcgagaaa agctggaaag
     3901 ccttgttatg gagtagagaa atttatggat caaattagtg ctattgatgc tattagttta
     3961 gaagaacagg cagatatgtt gtataaatct ttaacagata ctgctgaaaa tgatactatg
     4021 tctaaattaa atgaaatgtt ggaaacttat atgaattttg atttagataa aatgcttgaa
     4081 ttatcaacag attctactat gcccgcagat tttaatgaga attttttaat caaacgaaat
     4141 gttgttatgg ttaagaattt tcttaaaatt tctaaaaaac aatctttatt ttgtactgtt
     4201 ggtgcagcac acttagctgg tgaagatggt gttttggaat tattgagaaa gaaaggttat
     4261 actgtagaac cagtattttt caagtggact gaaaattaaa aaaaacattt aaaaaaaatg
     4321 gctactcaat cggtagccat tttttattta cagtattata tagcttattt ttgttcagct
     4381 aatcgtttgt agaaattgta tctccatttt gcatttcttt cagcttcttg taataaagct
     4441 tctgcagttt ctgggaacaa tttttttagt gagttgtaac gaacttcacc atcaaggaat
     4501 gtttggaatt tgctccaatc tggttctttg ctatctaatt ggaatggatt ttttccttca
     4561 gcttcaagtt gtggattgta tctccaatta acccaatatc cacattcaac agcacgtctc
     4621 tcttcgtctt gagttttgcc cattcctgct tttaagccat gattaataca tggtgcataa
     4681 gcaataatta gagatggtcc aggatatgct tcagcttctt taattgtttt ataatactga
     4741 gcatgattag cacctacagc aacttgtgca acataaacat aaccataagt cattgccatt
     4801 gcaccaaggt cttttttgcg agttttcatt cctgatgcag caaatttagc aattgcagct
     4861 gtaggagttg atttagaagc ttgtccacct gtgtttgagt aaacttctgt atccattact
     4921 aaaatattaa catctttgcc agaagcaata acgtgatcta gaccgccata acctatatca
     4981 taagcccaac cgtctccacc aaatatccat actgattttt taacaagata ttgttttttg
     5041 ttcattactt cttttgcaat tgggtcgttt agcttttcaa ttgctttaac tagtctttca
     5101 gatgctttct cagatgcttc tgcgttgtcc atatttgcta gccaatcttt agccgcttct
     5161 gctgcttcgc ctgtccattt agcttcgttt aaacgcattg caatgccttc tctcattttt
     5221 tctatagccg atgccatacc aaatccgtat tcagcattgt cttcgaatag tgagttcgcc
     5281 caagcaggac ctttacctga attgtagttt ttgcaatatg gagttgaagg agccgaacca
     5341 ccataaatag aagaacaacc tgtagcattt gctataatca ttctttcgcc aaataattga
     5401 gtaattaatt taatatatgg agtttcgcca catcctgcac aagcaccaga gaactcaaat
     5461 aatggttgag cgaattgtga gtttttaaca tttgagtgtt tgtcaactac attttctttg
     5521 taaccaacag tttcgtccat ataataccag cgatctactt cgtccatttg agtttcaagt
     5581 ggtttcatca ctaaagcttt agtttttgca ggacaaatat cagcacagtt tccacaacca
     5641 gtacagtcta aaacagaaac ttgcattttg aacttatatt cttttgttgg tccagcacct
     5701 tgaatagttt ttgttccagc aggagctttt ttaacttctt cgtctgttag taagaaagga
     5761 cgaatagctg cgtgaggaca aatataagaa cattggttac attggataca attttcagga
     5821 atccattctg gaacgttaac agcaacgcca cgtttttcat atttggttaa tccagctggg
     5881 aaactaccat tttcgcgacc gttaaatgtt gaaactggta aagtgtctcc tttttgagaa
     5941 ttgataacgt cagcaacttt tgaaacaaat tcaggaactt ctctatctct tttaaacttg
     6001 aaaccttcag ttttaatatt agcccattct gctggtacat caacttgaac aacgcttgct
     6061 ccagcatcaa cagctttata gttcatttga actatttttt caccttttaa accataagaa
     6121 atatcaatca ttttcttcat ttgttcaaca gctttttcgt aagggattac attagcaatt
     6181 ttgaagaatg ctgattgtaa aatggtatta gttctatttc ctaaaccaat ttcttctgca
     6241 atttgtgtag catttataat atagaattta atatttttct ctgctagaac ttttttcatg
     6301 tggtcaggaa gattcttttt agtttcttcc gcgtcatgaa gtgagttcaa taagaaactt
     6361 ccattaggtt taatgccttc tagtacatca taaagttcta catatttagg aacgtggcaa
     6421 gcaacaaaat caggtgtagt tactaaataa ggagctctaa tttctttgtc gccaaagcgt
     6481 aagtgagaag ctgtgaaacc tccagatttt ttagagtcat aagcaaaata tgcttgacaa
     6541 tatttatctg tgctatcacc aataatttta attgagtttt tgttagcacc aacagtacca
     6601 tcagaaccta agccgtaaaa ttttgcttcg aaagttcctt tatcaagaat agaaatgttt
     6661 tcttttaatg gtaatgataa atttgtaaca tcatcaacaa ttcctacagt aaatctatct
     6721 tttggtttgt ctgatgctaa attttcgaaa acagaaatca tcattgcagg agtagtgtct
     6781 tttgaagaaa gtccatatct accacctata atcattggtc tgttttcttt attatagaac
     6841 atttctttaa cgtctaagaa taaaggttcg ccgttagcac caatttcttt agttctgtct
     6901 aaaacagcaa ttctttttac agtttttgga agaactttca taaaatattt ttcagaaaat
     6961 ggtctgtata agtgtactgt aataacgcca acttttttac ctgaattgtt aaggtaatct
     7021 acagtttcga ttagcaggtc tgtaatagaa cccatagcta caataatgtt ttcagcatca
     7081 gctgcaccat gatatgtaaa tgggtgatat tctcttcctg ttaattttgt gatttcttgc
     7141 atatactcct caacaaggtc aggaatgttg gtgtaaaatt tatttgcagc ttcgcgagat
     7201 tggaaataaa tatctgggtt ttgagcagta cctttagtta caggattgtc tggattttga
     7261 gcagattttc taaaattatc caaagcttcc caatcaagta gtgcagccat ttcttcgttg
     7321 tcgaaatatt caactttttg aatttcgtga gaagttctaa aaccatcgaa gaagtgcata
     7381 aaaggaactc ttgatttaat tgctgctaag tgagcaatag gagctaagtc cataatttgt
     7441 tgaacagagc tagttgctaa cattgcaaaa cctgtttgtc tagtactcat aacatcgcta
     7501 tggtcgccaa aaattgacag tgcttgagca gctaaacttc ttgcactaac atgaaaaaca
     7561 gcaggtaata attcgcctga cattttgtac atattaggaa tcattaataa caagccttga
     7621 gaagcagtgt aagtggtggt taaagcacca gcttgtaacg caccgtgaac agctcctgca
     7681 gcaccggctt cagattgcat ttcttcaact tttactgttt gaccaaaaat gttttttctg
     7741 ccgtgagcag cccattcgtc aacgttctct gccatgtttg aagatggagt gataggataa
     7801 atacaagcca tatcactaaa catgtatgct acgtgtgaag cagcatggtt tccatcacat
     7861 gtgataaatt ttttttcttt tgacatattt gttttattaa ttaaatattt aaataatttt
     7921 attactatta atttcaattt taaaagtgtc gctaagttaa tatttttaat tgtcttaaaa
     7981 acagattttt tatgttttat attataaatt cctaatatag aaattgtcta aataagccac
     8041 aggaaaatat ttagaaaaat cgtaaaaata aaattttatt atattaatat tatatggcaa
     8101 aattgcttgt ttaaaggttc ttactgtatg gttttttgga aatatgtaaa aatttattat
     8161 atttgcacaa aaagtaacaa aagtgttatg gtttatattt taaatagctt agtgaaatta
     8221 ttgttattcc cattatttta tttttacact aacgctctat tctgtatttt ttcaatatat
     8281 ttctttaaaa tagacataaa catgttttta cagtttatga gcaatgcttt tacctaaagt
     8341 aagtgctttt tatttaatcc attaatttaa tacaaataaa acaaatatgt caaaagaaaa
     8401 aattataaaa aaaagaaaaa gtgataaaat tgaaaaactt ggaaaatcaa ttatacagca
     8461 tggaaaacta aatgatagag ttatcttatt gagttttaac cctaactccg tccttttagg
     8521 tagtctatat ttattttttc aaataatt
//
LOCUS       DAOPNX010000101         8487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_9332_length_8487_cov_7.850925, whole genome shotgun sequence.
ACCESSION   DAOPNX010000101 DAOPNX010000000
VERSION     DAOPNX010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8487
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9332_length_8487_cov_7.850925"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PKN53_09585"
     CDS             <1..723
                     /locus_tag="PKN53_09585"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89239.1"
                     /translation="FKVDTQLYYFASLAALKAGKFEEAIGYNEMLIQLGFGANDDEKV
                     AIYQSQAVAYRETGDTAKMLKVLEKGIDAFPQANFPLVIETFNYYVNIGKSDKAFEYI
                     NLAIEKNPSDAQFYVIRGTLLEEMKRKDEAKLDYVKAVELQSDNFDANYSLGAFYYNS
                     GVDTLDWADKNIPINKFSELGKYQEISNQLFEEALPYLEKAYAIQEKNVAVLSTLKTI
                     YYRLGKQDKYTEVDEKLKSLVE"
     gene            803..2308
                     /locus_tag="PKN53_09590"
     CDS             803..2308
                     /locus_tag="PKN53_09590"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOD89240.1"
                     /translation="MKKASLFLGLIFLGFFANSQNSIYLDFEILQNGTQLVLDSVLVE
                     NLDNGSDTTIYNFQSGLSFEMPIGVFPVFAENNELMIKQNYPNPFANETIIEINSPNE
                     NVDVAVYDISGKLVLSERLNVGKGKQIFYLNPGAAGKFIVAFKSEKQEKAIAVTSSAT
                     EQTKASIRWQGSTLNFMKKKEQNAFFIYNEGDVLKFTGFVTACEQAESISIVDSPTVS
                     QTYTFDFTYLQNIQPNAPIIENVTIAETSILWEFNSDENTIGYKYNFEPNYNTAIDIG
                     EATSLSKEELLPGTYYDFWLWAYNDCGASMPTKLSNATKALLFSADENSLILSGATTE
                     KMNILSICDENDTLILRNISTNVDLADENLAHLIERMRITVLNKGVGIAAPQVGLNRN
                     IVWIQRYDKGSAFNKPWECYINPRITAYSTEYFLRNDGCLSVGEECVNEYGIAGNSWR
                     ANWVDVEYYLPDGTFVQETITHVYTAHIFQHELDHLNGIMYFDRQTEPGAK"
     gene            complement(2311..3327)
                     /locus_tag="PKN53_09595"
     CDS             complement(2311..3327)
                     /locus_tag="PKN53_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOD89241.1"
                     /translation="MQRILIIYTGGTIGMIKNEKGFLQPFSFEHLINYIPLLKSFPAS
                     ISSYSFDELIDSSNADPDFWIKLSKIIIKNYDDYDGFLILHGTDTMAFTASALSFMLQ
                     NLSKPIILTGSQLPIDLIRTDGRENILAALELACMNENGTPLIKEVCVYFENTLYRGN
                     RTTKFSAENFDAFISPNFPSIAEVGIDIKLHKQNLLKTNSDKLIVENNFNNNIAILKF
                     FPGIKAETVEAICNIKNLRGLIIETYGSGNAPTDDKILKEIEKVINKGLVVLNISQCT
                     VGKVQQGKYETSVKLNDIGVVSGKDMTIEAATTKLMFVLGKNLLQSEMAKLLENSIAG
                     EISE"
     gene            3475..5601
                     /locus_tag="PKN53_09600"
     CDS             3475..5601
                     /locus_tag="PKN53_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008647187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOD89242.1"
                     /translation="MEIILDLVAQTTGISIKQVKATSLLLEEGATIPFISRYRKERTG
                     GLNEEDVEKIQKQKAYFQEIAERKETVLNSIEKQGKLTDELKQAIENTWDINVIEDLY
                     LPYKQKRKTRASIARENGLEPLSEIIFKQQNIDINKIASSFLNDNVPSVEDAIQGAKD
                     IIAEQISENAQARERVRKIFSSEAIITSKVVKSKIEEAEKYQDYFDFSQELSKCPSHR
                     ILAMRRGEREGFLRIDISPDKNIAFKSLKYLFVKGKTNTSALVEEAMVDAYDRLLKPS
                     IENEFANLSKESADILAIEVFADNLRQLLLAPPLGKKRILGIDPGYRTGCKLVCIDEQ
                     GNLLHNETIYPHKPQEERAIAMKKLSSLVNSYKIDAIAIGNGTASRETESLVKHISFD
                     REVRVYVVSEDGASIYSASSLAREEFPQYDVTVRGAVSIARRLMDPLAELVKIDPKSI
                     GVGQYQHDVDQKLLKDSLDRVVESAVNKVGVNLNTASKQLLTYVSGLGPVLAQNIVDY
                     RKENGAFKNRKELTKVPRLGAKAFEQAAGFLRIPDSTHALDNTAVHPESYHIVEKISK
                     ELNKSIEELIEEPNLLKTVNIDKYLTEEFGLPSFELIISELEKHGRDPRSTIKVMEFD
                     KTIYKIEDLKEGMVLPGIVTNITNFGAFVDIGIKENGLVHISQICNEFISSPNEVLKL
                     HQHVKVKVTNIDIPRKRIQLSMKNIE"
     gene            5628..7457
                     /locus_tag="PKN53_09605"
     CDS             5628..7457
                     /locus_tag="PKN53_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOD89243.1"
                     /translation="MSKKIIDLENVVIKFAGDSGDGIQLAGAQLTNTTAQLGEEVATF
                     PDYPAEIRAPQGTVGGVSGFQLHFGRHVNTPGDKCDVLIAFNPAAIMANINSIKWGGI
                     IVADIDAFTEANMEKAGFKDDTMDHIRQNFDLIEAPISSMSKESLKGIEDLDQKTILK
                     SKNMFALGLVYKLFGFPLSYSEEFFDKKFKNKPKLAEVNKKVLQGGASYASAIQAIPS
                     FRLCGEKKLKGRYRSISGNVATAWGLLAAAEKANLPFFLGSYPITPATDILQEIANRK
                     DVGAKVFQAEDEIGGIVTAIGASYAGNLAATSTSGPGLALKSEALGLAVITELPLVVV
                     DVQRGGPSTGLPTKTEQADLFQALTGRNGESPVPVIAASTPSDCLHYAFMASKFALEH
                     MTPVILLTDSFLANGTEPMSIPEMKKFPEIKTKLVPKDQEKYLPYERDENLVRGWAIP
                     GMKGFEHRVGGLEKMDITGAVSYVPANHERMVYLRKQKVENMAKFYPEQKVEGSEDAD
                     ILVIGWGSTYGHIKTTVEELRKEGKKVAHAHFNYINPLPRNTDDILKKYKKILVCELN
                     SGQFANYLRMKFPKYKYLQYNKVMGLPFAVAELKEEITKVMEA"
     gene            7462..8472
                     /locus_tag="PKN53_09610"
     CDS             7462..8472
                     /locus_tag="PKN53_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOD89244.1"
                     /translation="MAENKYTVQDFKSSVQVRWCPGCGDFSVLASVQKAMSELNYKKE
                     DYAVISGIGCSSRFPYYMNTYGFHGIHGRAGVLASGVKVANPKLSVWQITGDGDALAI
                     GGNHFIHEIRRNIDLNVILFNNEIYGLTKGQYSPTSKFGQITNSSPFGTIEKPFNAGR
                     LTLGAGGNFFARTIDANVKETVEILKAADQHKGTAIVEVLQNCVIFNNGAHQRITDKE
                     ERENNQLWLEHGKPMIFGKDRNKGLVLDGLKLKVVTIGENGITEKDILVHDMYEEDLT
                     LHTMLVNLNLPDFPVVFGIVRAVKAATYNDLLYEQIAEVQSKSSIKGMDDVFNSGSTW
                     EV"
BASE COUNT         3134 a         1153 c         1520 g         2680 t
ORIGIN      
        1 tttaaagtag atactcaatt atactatttt gcttcgctag ctgcactaaa agctggtaaa
       61 ttcgaagaag ctataggata taacgaaatg cttattcaat taggatttgg agctaacgat
      121 gatgaaaaag tagcaattta ccaaagtcaa gctgttgctt atagagaaac aggcgatact
      181 gcaaaaatgc ttaaagtttt agaaaaaggt atagatgctt ttccacaagc taactttcca
      241 ttagtaatag aaacttttaa ctattatgtt aatattggaa aaagtgataa agcatttgaa
      301 tatataaatt tggctataga aaaaaatcca agcgatgctc aattttatgt tatcagagga
      361 actctattag aagaaatgaa acgtaaagac gaagctaaac ttgactatgt taaagctgta
      421 gaattacaat cagataattt tgatgctaat tattcactag gagcatttta ctataactct
      481 ggtgttgata ctttagattg ggcagataaa aatattccaa taaataagtt ctctgaatta
      541 ggtaaatatc aagaaatttc aaatcaacta tttgaagaag ctttgccata cctagaaaaa
      601 gcttatgcta tacaagaaaa aaatgttgct gtattaagta cattgaaaac tatttactat
      661 agacttggaa aacaagataa atataccgaa gttgatgaaa aattaaaatc gctagtagaa
      721 tagatttaaa ataatattat aaaaaaaggc tggttttcag ccttttttta ttaattttgt
      781 attgtaatta aattttttta aaatgaaaaa agcaagttta tttttaggat taatctttct
      841 tggatttttc gcaaactccc aaaactctat atatttagat tttgagattt tacaaaatgg
      901 tacgcaacta gttctagata gcgttttagt tgaaaattta gataatggct ccgatactac
      961 tatttataat tttcagtcag ggctaagttt tgaaatgcct ataggagtgt ttcctgtttt
     1021 tgccgaaaat aatgagttga tgataaaaca aaattatcca aatccttttg ctaacgaaac
     1081 aataattgaa ataaattctc caaacgaaaa tgttgatgtt gctgtttacg atataagtgg
     1141 taaacttgtt ttatcagaaa ggttaaatgt tggaaaaggt aaacaaatat tttacttaaa
     1201 tccaggagct gctggtaaat ttattgttgc atttaaaagc gaaaaacaag aaaaagcaat
     1261 tgctgttaca agtagtgcaa cagaacaaac taaagcttca attcgttggc agggaagtac
     1321 gctaaatttt atgaagaaaa aggagcaaaa tgcttttttc atatataacg aaggtgatgt
     1381 attaaaattt acaggtttcg ttactgcttg cgaacaagct gaatcaatat caattgttga
     1441 cagcccaacg gtttctcaga cttacacatt tgattttact tatttgcaaa atattcaacc
     1501 aaatgcacct ataattgaaa atgttacaat tgctgaaact tcaattttgt gggaatttaa
     1561 ttcagatgaa aatacaattg gttataaata taattttgaa cctaactata acaccgcaat
     1621 agatatagga gaagcaacta gcttgagtaa agaagaatta ttaccaggca cttattatga
     1681 tttttggtta tgggcttaca atgattgtgg tgcctcgatg ccaacaaaat tgtcaaatgc
     1741 taccaaggct ttgttattct ctgctgatga aaattctttg attttaagtg gagcgacaac
     1801 agaaaaaatg aatatattaa gcatttgtga tgaaaatgat actttaatcc tacgcaatat
     1861 ttcaactaat gttgatcttg cagatgaaaa cttagcacac ttaatagaga gaatgcgcat
     1921 aactgtctta aataaaggtg ttggtatagc agctcctcaa gttggtttaa atagaaatat
     1981 cgtatggata caacgttatg ataaaggtag tgcttttaat aaaccttggg aatgttacat
     2041 aaatccaaga attacagctt attctacaga atatttttta agaaatgatg gctgtttgtc
     2101 agtcggcgaa gaatgtgtta atgaatatgg aatcgcagga aactcatggc gtgcaaattg
     2161 ggttgatgtt gaatattatt taccagacgg aacctttgtg caagaaacaa ttactcatgt
     2221 atataccgca catatttttc aacacgaatt agaccatttg aatggaataa tgtattttga
     2281 tagacaaaca gagcctgggg caaaataata ctactcacta atctcccctg caatagagtt
     2341 ttctaaaagt tttgccattt cactttgcag aagatttttt cctaatacaa acattagttt
     2401 tgttgtagct gcttcaatag tcatatcttt gccactgaca acgcctatat cgtttagctt
     2461 aacgctagtt tcatattttc cttgttgaac ttttcctact gtgcattgag aaatatttaa
     2521 aacaactaat cctttattaa ttactttttc aatttcttta agaattttat cgtctgtagg
     2581 agcattgcct gagccataag tttcaataat taaacctcta aggtttttta tattgcaaat
     2641 tgcttcaact gtttcggctt taattccagg gaaaaatttt aaaattgcaa tattgttatt
     2701 aaaattattt tcaacaatta atttatctga atttgttttt agcaagtttt gtttatgtag
     2761 ctttatatct attccaactt cagcaatact tgggaaattt ggagaaataa aagcatcaaa
     2821 attttcggca ctaaacttag tagttctatt tcctctgtat agagtgtttt cgaaataaac
     2881 acaaacttct ttaattaatg gtgtgccatt ttcattcatg caagctaatt ctaatgctgc
     2941 taaaatattt tcacgtccat ctgttctaat taaatctatt ggtaattgag aacctgttag
     3001 aattattggt tttgataaat tttgtagcat aaacgataaa gcagaagccg taaacgccat
     3061 cgtgtctgta ccatgcagaa ttaaaaaacc atcataatca tcgtagtttt taatgataat
     3121 tttgcttaat tttatccaaa aatctgggtc tgcatttgag gaatctatca attcatcaaa
     3181 actatatgaa cttatactgg ctggaaaaga ttttaataaa ggaatataat taattaaatg
     3241 ctcaaaacta aatggttgta aaaagccttt ttcgtttttg atcattccaa ttgtgccacc
     3301 tgtgtaaatt attaaaattc tttgcatgaa ttagatgttt ttttgcaaag ctaaaatttt
     3361 taaacgtaat cttaaaataa aactgaaatt aaattttata atttaaagca aaaagaactt
     3421 ataaaacata tatacttaat aataaaatga ttatgtttgc acaaaaataa ttaaatggaa
     3481 atcatattgg atttggtagc ccaaactacc ggaatttcaa ttaaacaagt aaaagccaca
     3541 tctcttctat tagaagaagg tgcgactatt ccatttattt ctcgatatcg taaagaaaga
     3601 acaggtggtt taaatgagga agatgtcgaa aaaattcaaa aacaaaaagc ttattttcaa
     3661 gaaattgctg aaagaaaaga aactgtttta aattcaatag aaaaacaagg aaaattaact
     3721 gacgaattaa agcaggcgat agaaaatact tgggacataa atgttatcga agatttgtat
     3781 ttgccatata agcaaaaaag aaaaactagg gcttcaattg ctagggaaaa tggcttagaa
     3841 cctctgtcag aaataatttt caaacaacaa aatatagata taaataaaat cgcttctagt
     3901 tttttaaatg ataatgtgcc tagtgtagaa gatgcaattc agggagcaaa agacattata
     3961 gccgaacaaa ttagtgaaaa tgctcaagca agggaaagag ttagaaagat tttcagctca
     4021 gaagcaatta ttaccagcaa agttgtaaaa tcaaaaattg aagaagcaga aaaatatcaa
     4081 gattattttg atttttcaca agaattgtca aaatgccctt ctcatagaat acttgcaatg
     4141 cgaagaggag agagagaagg ttttttgaga atagatattt cgccagataa aaatattgct
     4201 tttaaaagtt taaaatatct ttttgttaaa ggaaaaacaa atacttcggc tttggttgaa
     4261 gaagctatgg ttgatgccta cgatagattg ttaaaacctt ctattgaaaa cgaatttgct
     4321 aatttaagta aagaaagtgc agatattttg gcaattgaag tatttgcaga taatttacgt
     4381 caattattac tagcaccgcc tttaggaaaa aaacgtattt taggaataga cccaggatat
     4441 agaacaggtt gtaagcttgt ttgtattgat gagcaaggaa atttgttgca taacgaaact
     4501 atttatccac ataaacctca agaagaaaga gctatagcaa tgaaaaaact tagttctttg
     4561 gtaaatagtt acaaaattga tgcaattgca ataggaaacg gaactgctag tcgcgaaacc
     4621 gaaagccttg ttaaacatat ttcgtttgat agagaagtta gagtttatgt ggtaagcgaa
     4681 gatggagctt caatttattc tgcttctagt cttgctcgtg aagaatttcc acaatatgat
     4741 gtaactgttc gtggagcggt gtctattgct cgtcgtttga tggatccttt agctgagcta
     4801 gtaaaaatcg acccaaaatc aataggagta gggcagtatc agcatgatgt tgaccaaaaa
     4861 cttttaaaag atagtttgga tagagtagta gaatcggcgg ttaataaagt tggagtaaat
     4921 ttaaatactg ctagcaagca actgcttact tatgtttcgg gtctaggacc agttttagct
     4981 caaaatattg tagattatag aaaagaaaat ggagctttta aaaatcgaaa agaacttact
     5041 aaagtgccaa gattaggagc taaagctttt gaacaggcag caggtttttt aagaatacca
     5101 gattctacac atgctcttga caacacggcg gttcatccag agtcatatca tatagttgaa
     5161 aaaatttcta aagaattaaa taaatcaatt gaagaattga ttgaagaacc aaatttatta
     5221 aagacagtaa atattgataa atatttaaca gaagaatttg gtttaccaag ttttgaactt
     5281 ataatttcgg aattagaaaa acatggtcgc gatccgcgaa gcaccattaa agtaatggaa
     5341 tttgacaaaa ccatttataa aatcgaagat ttgaaagaag gaatggtatt gccgggaatt
     5401 gttacaaata ttacaaattt tggtgcattt gttgatatag gaattaaaga aaatggttta
     5461 gttcatattt cgcaaatttg taacgaattt ataagttcac caaatgaagt tttaaaactt
     5521 catcaacatg ttaaggttaa agttactaat attgatattc cgcgaaaaag gattcaattg
     5581 agtatgaaaa atattgaata acaaaattaa taaaataaat aataattatg agtaaaaaga
     5641 tcattgacct cgaaaatgtg gtaattaaat ttgcgggtga ttcaggagac ggcattcaac
     5701 ttgctggggc tcagttgact aacactacag ctcaactcgg agaagaagtt gctacttttc
     5761 ctgactatcc tgcagaaatt cgagcacctc aaggaactgt tggaggagtt tctggtttcc
     5821 aattgcattt cggaagacat gttaacacac caggcgacaa gtgtgatgtt ttaattgcgt
     5881 ttaatcctgc tgctattatg gcaaatatta attccataaa atggggtgga attatagttg
     5941 cggatattga tgcttttact gaagcaaata tggaaaaagc tggatttaaa gatgatacaa
     6001 tggatcatat tagacaaaac ttcgatttaa tcgaagctcc aattagttca atgtcgaaag
     6061 aaagcttgaa aggtatagaa gatttagatc aaaaaactat tttgaaaagt aaaaatatgt
     6121 ttgctttagg tctagtgtat aaattatttg gtttcccatt gagttattca gaagaatttt
     6181 ttgataaaaa atttaaaaat aaacctaaac tagccgaagt aaataaaaag gttttacaag
     6241 gtggagcaag ctatgcatct gcaattcaag caataccatc gtttagactt tgtggagaaa
     6301 agaaactaaa aggaagatat agaagcatta gcggaaacgt tgctactgca tggggcttgt
     6361 tggcagcagc agaaaaagca aacttgccat tctttttagg ttcttatcct atcacaccag
     6421 caacagatat tttacaagaa atagcaaatc gcaaagatgt tggtgcaaaa gtatttcaag
     6481 ccgaagacga aattggtggt atagtaactg caattggagc ttcttatgct ggtaacttag
     6541 cagctacttc aacatcagga cctggtttag cattaaaatc agaagcatta ggtttggcag
     6601 ttattaccga attaccattg gttgttgttg acgttcaacg tggaggacca tcaacaggtt
     6661 taccaacaaa aacagaacaa gccgatttgt ttcaagcttt aacaggaaga aatggagaaa
     6721 gtccagttcc tgtaattgct gcaagtacac catctgattg tttgcattat gcatttatgg
     6781 cttctaaatt tgcattagaa catatgacac cagtaatttt gttgacagat agtttcttag
     6841 caaatggaac agaacctatg agtattccag aaatgaaaaa attcccagaa attaaaacaa
     6901 aacttgttcc aaaagatcaa gaaaaatact tgccttacga aagagatgaa aatcttgtaa
     6961 gaggatgggc tatacctgga atgaaaggtt ttgaacatag agttggtggt ttggaaaaaa
     7021 tggatataac tggtgcggtt tcttacgttc cagcaaatca cgaaagaatg gtttatttga
     7081 gaaaacaaaa agttgaaaat atggctaaat tctatcctga acaaaaagtt gaaggaagtg
     7141 aagatgccga tattttagta attggctggg ggtcaactta tgggcatata aaaactacag
     7201 tcgaagaatt aagaaaagaa ggaaagaaag ttgcacatgc tcattttaat tatattaatc
     7261 ctttgccacg caatactgat gatatattga aaaaatataa gaaaattttg gtttgcgaac
     7321 taaactcagg acaatttgca aattatttga gaatgaaatt cccaaaatat aaatatttgc
     7381 aatataataa agtgatgggc ttaccttttg cggtagctga attaaaagaa gaaataacaa
     7441 aagtaatgga ggcttaatat tatggcagaa aataaatata cagtacagga ttttaaaagt
     7501 tcagtgcaag ttagatggtg tccaggttgt ggcgactttt ctgttttggc atcagtacaa
     7561 aaagcaatgt cggaactaaa ttataaaaaa gaagattatg cagtaatttc tggaattggt
     7621 tgctcttctc gtttcccata ttatatgaat acttacggtt ttcatggaat tcatggacgt
     7681 gcaggagtat tggcttcggg agttaaagtt gctaatccaa aacttagcgt ttggcaaatt
     7741 acaggagacg gtgatgccct agctattgga ggaaatcact ttattcacga aattagaaga
     7801 aatatcgatc ttaatgtaat tttatttaat aatgaaattt atggattaac taaaggtcaa
     7861 tattcaccaa cttctaagtt tggacaaata acaaattctt ctccttttgg aacaattgaa
     7921 aaacctttta atgctggtcg attaactttg ggtgctggag gaaacttctt tgctcgcact
     7981 atagatgcta atgtaaaaga aacagtagaa attttaaaag cagctgatca acataaggga
     8041 acagctattg ttgaggtttt acaaaattgt gtaattttta ataacggtgc acaccaaaga
     8101 attacagaca aagaagaaag agaaaataat caactatggc ttgaacatgg caaaccaatg
     8161 atttttggta aagatagaaa taaaggatta gttttagatg gtttaaaact aaaagttgtt
     8221 actattggcg aaaacggaat tactgaaaaa gatattttgg ttcacgatat gtatgaagaa
     8281 gatttaactt tacatacaat gttagttaat cttaacttac cagattttcc agttgttttc
     8341 ggaatagttc gtgcagtaaa agcagcaaca tataacgatt tactttatga acaaattgca
     8401 gaagtgcaat caaaatcatc tataaaagga atggatgacg tgtttaatag cggtagcact
     8461 tgggaagtgt aataacaaaa ttttaaa
//
LOCUS       DAOPNX010000102         8462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_9357_length_8462_cov_8.319020, whole genome shotgun sequence.
ACCESSION   DAOPNX010000102 DAOPNX010000000
VERSION     DAOPNX010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8462
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9357_length_8462_cov_8.319020"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(280..1938)
                     /gene="recN"
                     /locus_tag="PKN53_09615"
     CDS             complement(280..1938)
                     /gene="recN"
                     /locus_tag="PKN53_09615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOD89245.1"
                     /translation="MLKSLQINNYLLIDNLEINFNSGFSVITGETGAGKSIMLDAISL
                     LLGKRADTDVLSDKSRKCVIEANFILNEDKFKNDFSKNDLDFEKSTIIRREILPEGKS
                     RAFINDTPVTLAVLKSIGDNIIDIHSQHENLNLGFMQFRTQIIDIAAGTKEMAEQFVE
                     KHNNLNKLETEIAKLKTQIANNTNEKEFTKFQIEQIEQAKINDENELEELEEKASFLE
                     NAVEIKENLYASINLARDVEFSAEERLKEIKNLLSKLSQKHKPAESLVDRLDSILIEF
                     KDIYSQIESDLYKAEINPELLEEINARIDLINGLLQKHKLANISELKNKYLELTNFIN
                     SSDTLEQELEEKEKEFKKLKAETIEFAEKLSAKRQKVFSKVEKEITELLVNLGIKHAN
                     FIIENIKDNKLSLHGFDNINFLFSANKAVAPQAIEKIASGGEFSRLMLALKSQTAHAA
                     AVPTIIFDEIDTGVSGEIANRMGKIMLDLGNSFQVIGITHLPQVAALGTTHYKIYKQS
                     DDKRTYTKVKMLNHEERITEIASIMSGEKLTSQAIDNARILLEN"
     gene            complement(1940..2848)
                     /locus_tag="PKN53_09620"
     CDS             complement(1940..2848)
                     /locus_tag="PKN53_09620"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HOD89246.1"
                     /translation="MKKIILIIILSVFVLLSKAQELDCRINLDHSRIPGTVNEKLFQT
                     LRQSLFELINNTNWTNHVFSRDERIECNIFITVEEQISSDEFNGRIQITSSRPVYGTS
                     YMTPVFNHIDDKFQFKYVENEPLEFNINSFTSNITSVIAYYCYIIIGMDYDTFGNSSG
                     TPYFQLAEKIVQNAQSAQEPGWKAYESLKNRYWLVENLLSSQYSEMREFMYDYHRRGM
                     DKLAEKPAEGRNSIEEAINKLKNIHRKKPGSFLMSIYTEAKRDEIINVFSEGFPDEKA
                     RVYNIMKEADPANATKYEKIMKEPNY"
     gene            complement(2845..4053)
                     /gene="coaBC"
                     /locus_tag="PKN53_09625"
     CDS             complement(2845..4053)
                     /gene="coaBC"
                     /locus_tag="PKN53_09625"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOD89247.1"
                     /translation="MLKGKKILLGITGSIAAYKSASIVRLLVKNGAEVKVVMTPISKE
                     FIKPLTLATLSKNPILVDFFDPENGSWNSHVDLGLWADAFLIAPATANTMGKMANGIA
                     DNLLLTCYLSAKCPVFIAPAMDLDMFLHPANQKNIEVLKSFGNFFIEPKTGELASGLI
                     GKGRMEEPEAIVQFLVEFFEKKKTLKNKKVLITAGPTQEKIDAVRFISNYSSGKMGYA
                     LAEECANRGAEVTLISGPVHIQAKHKNINKISVVSADEMFEATMQNLKHVDIIILAAA
                     VSDYKAQNISSNKIKREQNNIEIKLEANKDIAAEIGKLKTKNQISIGFALENQNEVEN
                     AQKKLKKKNFDFIVLNSMNDKGAGFNFDTNKIMIINNNNEVLKFNLKTKDLVAKDIID
                     NLEKILNSKS"
     gene            complement(4059..4406)
                     /locus_tag="PKN53_09630"
     CDS             complement(4059..4406)
                     /locus_tag="PKN53_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004349573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOD89248.1"
                     /translation="MDIKKIDAPVSTSTKNIVEIAEKTGNVYEAVVIIAKFADQINAD
                     LRKELYEKLEEFATYTDTLEEIHENREQIEVSKYYERLPKPSLIALEEFLADNIHYYY
                     AKETDSDNSEERL"
     gene            complement(4396..5208)
                     /gene="bamD"
                     /locus_tag="PKN53_09635"
     CDS             complement(4396..5208)
                     /gene="bamD"
                     /locus_tag="PKN53_09635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOD89249.1"
                     /translation="MFKLNVRNILILLVIVALANSCGEYSKLVKSSDYELKYTKAIEY
                     YEAGEYYKAQALFEDLKGIYRATDKAQKVAYYNAYCSYGLGEYSLAAYLFKDFVRVYP
                     ASEHAEEVSYIAAYCYYLNSPEVSLDQTFTKAAIAELQFFVEKYPNSEKRAEAEKHID
                     ELQFKLETKAFDNAMLFYNIMDYKAATTALRNVLKDFPDTKYREEIMFTIMKANYLLA
                     ENSIVAKQYERYQETIEDYYAFVDKFPNSKKIKEAEKIFTNSVKFIESKNGH"
     gene            complement(5301..5996)
                     /locus_tag="PKN53_09640"
     CDS             complement(5301..5996)
                     /locus_tag="PKN53_09640"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOD89250.1"
                     /translation="MLLSDYVKKIQEFFEKYRNEEEAEPMSKYMRYKFPFIGIPASKR
                     NKLFNLFFNTFPIPDYDETKAIVRELFNLPEREYHYFAIHLLIKYKKKWVPTDIVFFE
                     SLILNKSWWDSVDLISSKLVNPFFLKYRNLEEAMTYSWSRSNNIWLKRVSIIYQNGYK
                     KETNLDILTRHILENADSNEFFIQKAIGWALRDYSKVDYKWVIDFVVNNTKLKPLSKR
                     EAIKWIDNKGLIQ"
     gene            6145..7263
                     /gene="lpxK"
                     /locus_tag="PKN53_09645"
     CDS             6145..7263
                     /gene="lpxK"
                     /locus_tag="PKN53_09645"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOD89251.1"
                     /translation="MREFVKILKILCLFPLMLIYRSVVTFRNFLYDSKILKSHEFDMP
                     IISVGNISVGGSGKTPHTEFLIEMLQDEYQVAVLSRGYKRKTRGFRLVEADDSFLLSG
                     DEPLQIKQKFPNAIVAVCENRVKGISQLMSKFPDLKLIILDDAFQHRRVKPGLSILLN
                     NFNHPINKDFLLPLGYLREPRSSAYRAHIVVFTKCPNKLKPIEKRILIKESEVYPYQY
                     LFFTTYNYLDLKPVFNEDKKTIPISDLKKYNVVLFSGLAKHTSLYNFFKENSKSVKHI
                     KFPDHHNFKKRDIIKIEKEFKNTKEPSIIVTTEKDAVKLKDNKYFAEETKKMLYCAPI
                     KVEQLGSEEDIKQFNNQIFSYVRNNKRYCKLYKNAYSG"
     gene            7208..8017
                     /locus_tag="PKN53_09650"
     CDS             7208..8017
                     /locus_tag="PKN53_09650"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOD89252.1"
                     /translation="MLEIIKDTVSYIKTHIQVEPEIGIVLGTGLGGLVKEIDIHTSLS
                     YEKIPNFPISTVEGHSGKLIFGKLGGKNVVAMQGRFHYYEGYDMNQVVFPIRVMKYLG
                     IKTLFLSNASGGVNPDFEIGDLMIITDHICLVPNPLIGKNEPEFGTRFPDMSEAYDKN
                     LIAKAKQVAQNLGIKVTQGVYTATSGPTFETPSEYKYFRIIGSDTVGMSTAPEVITAR
                     HLGLPCFAMSIITDLGVPGKIVEVTHEEVQKVAAAAESKMTQIFKQMIAEI"
     gene            8083..>8462
                     /locus_tag="PKN53_09655"
     CDS             8083..>8462
                     /locus_tag="PKN53_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89253.1"
                     /translation="MENDILHINSKFYWASLILIILVLSFSCSSTKYVAEGDYLLAST
                     KINIEGSSDIDKEDLESSLRQKPNKKVFGLRFYLMVYNSINQDKEKLREEKRKVKDAE
                     RNAKRKAKGKKPKDRFYITRWLSNL"
BASE COUNT         2786 a         1253 c         1249 g         3174 t
ORIGIN      
        1 ataagcccgc tctctcggat tttcccgcac aaccccgctc gctcggattt gcaatccgag
       61 tgtaacactt ccaaacactc ggattacaaa tccgagcgag cgtcagaggt gttagagcga
      121 gcgtcagagg tgttagagcg agcgtcagag gtgttagagc gagcgtcaga ggtgttagag
      181 cgagcatcag tggcgttgaa aacgagcaaa ttgtaactaa atccgagtga gcagaatgaa
      241 tagatgaatt taaaaatctt gatgaggagt agtaagttat taattttcta acaatattct
      301 agcattatca atagcttggc tagtgagctt ttcgccactc attatgctag ctatttctgt
      361 aattctttct tcgtgattta acattttaac tttggtgtaa gttcttttgt catcactttg
      421 tttataaatt ttataatgag ttgttcccaa agcggctact tgtggcaaat gtgtaatacc
      481 aattacttgg aaactatttc ctaagtcgag cattattttt cccattctgt ttgcaatttc
      541 gccagaaact cctgtgtcaa tttcatcaaa aataattgtg ggaacagcag ctgcatgtgc
      601 tgtttgggat tttaatgcta acatcaaacg agaaaattct ccacccgaag ctattttctc
      661 aattgcttgc ggagcaacag ctttgtttgc tgaaaataaa aaattaatgt tgtcaaaacc
      721 atgtaaacta agtttgttgt ctttaatatt ttcaataata aaattagcat gttttattcc
      781 taagtttaca agtagttcag taatttcttt ttcaactttt gaaaatactt tttgtctttt
      841 tgcacttaat ttttcagcaa actcaattgt ttcggctttt aattttttaa attctttttc
      901 tttttcttct aattcttgct ctaaggtatc actagaattt atgaaattag ttaattctaa
      961 atatttattt tttaactctg aaatattagc aagtttatgt ttttgcaaaa gtccatttat
     1021 caagtctatt ctagcgttga tttcttctaa aagttcagga tttatttcag ctttgtataa
     1081 atcgctttct atttgagaat aaatatcttt aaattctatt aaaatagaat caagtctatc
     1141 aacaagactt tcggcaggtt tgtgtttttg agagagttta gaaagtaaat tttttatttc
     1201 ttttaatctt tcttcggcag aaaactcaac atctctagca agatttatag aagcgtaaag
     1261 attttctttt atttctacag cattttctaa aaacgaagct ttttcttcaa gttcttcaag
     1321 ttcgttctca tcatttattt ttgcttgttc aatttgttca atctgaaatt ttgtaaactc
     1381 tttttcgttt gtattatttg caatttgtgt ttttagttta gcaatttcag tttcaagctt
     1441 attcaaatta ttgtgttttt cgacaaactg ttcagccatt tctttagttc ctgctgctat
     1501 atcaataatt tgtgttctaa attgcataaa acctaaattc aagttttcgt gctgagaatg
     1561 aatatctata atattgtcgc ctattgattt taaaacagca agcgttacag gcgtatcgtt
     1621 tataaaagct ctggattttc cttcgggtaa aatttctcgc cttattattg tagatttttc
     1681 aaaatctaaa tcatttttac taaaatcgtt tttgaattta tcttcattta aaataaaatt
     1741 tgcttcaata acacattttc gagatttgtc gcttaagaca tcagtgtcgg ctctttttcc
     1801 aagcaataat gaaattgcat ctagcataat tgattttcct gcacctgttt cgccagtaat
     1861 tacagaaaaa ccagagttaa aattaatttc taaattgtca attaataagt aattgtttat
     1921 ttgcaacgat tttaacatac taataattag gctctttcat tattttttcg tactttgttg
     1981 catttgcagg gtcggcttct ttcataatat tatatacgcg tgctttttca tcaggaaatc
     2041 cttctgaaaa aacattaata atttcgtctc ttttagcttc tgtatatata ctcattaaaa
     2101 aagaaccggg tttttttcta tgaatatttt ttaatttatt tattgcttct tcgatgctat
     2161 ttcgtccttc tgcaggtttt tcggctagtt tgtccattcc tcttctatga taatcataca
     2221 taaactctct catttctgaa tattggctgc tcaaaaggtt ttcaactaac caatatctat
     2281 tttttaggct ttcgtatgct ttccaacctg gttcttgtgc cgattgagcg ttttgaacaa
     2341 ttttttcggc taattgaaaa tatggagttc ctgaactatt accaaaagtg tcataatcca
     2401 ttccaattat tatatagcaa taatatgcaa ttacagaagt tatgtttgag gtaaaggaat
     2461 taatattaaa ttctagaggt tcgttttcaa catatttaaa ctgaaattta tcatctatat
     2521 ggttaaaaac tggtgtcata tacgaagttc cataaacagg gcgagatgag gttatttgaa
     2581 ttcttccatt aaactcatct gaagaaattt gttcttctac agtaataaat atattacatt
     2641 ctattctttc gtcacgcgaa aaaacgtggt ttgtccagtt agtgttgtta ataagctcaa
     2701 acaatgattg tcgcaaagtt tgaaatagtt tttcgtttac agttcctgga attctagaat
     2761 ggtctaagtt aattctgcaa tcaagttctt gtgctttcga aagcaaaaca aaaacagata
     2821 atattattat taaaataatt ttcttcatga tttagagttt aaaatttttt ctaaattatc
     2881 aataatatct ttagcaacca aatcttttgt ttttaaattg aactttaaaa cttcattatt
     2941 attgttgata atcataattt tatttgtatc aaaattaaaa ccagcacctt tgtcattcat
     3001 agaatttagc acaataaaat caaaattttt cttttttaat tttttttgtg cattttctac
     3061 ttcattttga ttttctaaag caaaacctat gctaatttga tttttagttt ttaattttcc
     3121 tatttcagca gcaatgtctt tattagcttc aagtttaatt tcaatattgt tttgctctct
     3181 tttaattttg ttgctagata tattttgtgc tttgtaatca cttacagcag cagcaagaat
     3241 tattatgtca acatgtttta agttttgcat tgtggcttca aacatttcat ctgccgaaac
     3301 aacgcttatt ttatttatat ttttgtgttt tgcttgaata tgaactggtc ctgagataag
     3361 agtaacttca gcacctctat tagcacattc ttcagcaaga gcatatccca tttttccaga
     3421 agaataatta ctaataaatc ttactgcatc aattttttct tgtgttggtc cggcagtgat
     3481 taaaactttt ttatttttta aggtcttttt tttttcaaag aactcaacaa ggaattgtac
     3541 aattgcttcg ggttcttcca ttcttccttt tccaatcaag ccgcttgcta gttctccagt
     3601 ttttggttca ataaaaaagt ttccgaaaga ttttaaaact tctatatttt tttgatttgc
     3661 aggatgcaga aacatatcta agtccattgc tggagcaata aacacagggc attttgctga
     3721 taaatagcaa gttagcaaaa gattgtctgc tattccgttt gccattttgc ccatggtatt
     3781 tgctgttgct ggagcaatta aaaatgcgtc tgcccacaaa cctaaatcaa catgagagtt
     3841 ccaacttcca ttttcgggat caaaaaaatc aactaatatt gggtttttcg atagagtagc
     3901 caaagtaaga ggctttataa actctttaga tataggagtc ataaccactt ttacttctgc
     3961 accatttttt actaaaagcc ttacaatgct tgcagattta taagcggcta tactaccagt
     4021 aatgcccaat aagatttttt taccctttaa catttatttt aaagtctttc ttctgaatta
     4081 tcgctatcag tttcttttgc gtaataatag tgaatattat cagccaaaaa ctcttctaaa
     4141 gctataagtg atggcttagg aagtctttcg taatacttag aaacttcaat ttgttcacga
     4201 ttttcatgta tttcttcaag agtatctgta taagttgcga actcttcaag tttttcgtat
     4261 aattcttttc ttaaatctgc attaatttga tcggcaaatt ttgcaataat aacaacagct
     4321 tcgtaaacat tacctgtttt ttcggctatt tccacaatat tttttgtaga ggtagaaacc
     4381 ggagcgtcaa tttttttaat gtccattttt agattctata aatttaactg agtttgtaaa
     4441 tattttttct gcttctttaa ttttttttga atttggaaat ttgtcaacaa aagcataata
     4501 atcttctatt gtttcttggt atctttcgta ttgttttgca actatactat tttctgctaa
     4561 aagataattt gctttcataa tagtaaacat aatttcttca cgatattttg tgtctggaaa
     4621 atctttcaac acatttctaa gagctgtagt agcagctttg taatccatta tattgtaaaa
     4681 caacattgca ttatcaaaag cttttgtttc taatttaaac tgtaattcgt caatgtgttt
     4741 ttctgcttct gcacgttttt cagaatttgg atatttttct acaaaaaatt gtaattctgc
     4801 aatagctgct tttgtaaaag tttgatctaa cgaaacttct ggcgagttta aataataaca
     4861 atatgcagca atataactaa cctcttcggc atgttcgctg gctgggtaaa ctcttacaaa
     4921 atctttaaac aaataagcag ctaaagaata ttcgcctaag ccatacgaac aataagcatt
     4981 ataatatgca actttttgag ctttgtcggt agctctatag attcctttta aatcttcaaa
     5041 tagagcttga gccttataat attctccagc ttcgtaatat tctatagctt tagtatattt
     5101 taattcataa tctgaacttt taactaattt tgagtattcg ccacaagaat tagcaagtgc
     5161 aactattaca agtaaaatta aaatatttct aacgttcaat ttaaacattt tgcaaatata
     5221 taccaaatta atgaaatatt gtttaaaaaa agcgatttct gattaaaaaa attattaaga
     5281 taacttaaaa gtataaatta ttattgtatt aatcctttat tatctatcca cttaatagct
     5341 tcgcgtttac tcagaggttt taattttgta ttatttacaa caaaatctat tacccattta
     5401 taatcaactt ttgaataatc tcttaaagcc cagcctatgg ctttttgaat aaaaaattca
     5461 tttgagtctg cattttcaag aatatgtctt gttagaatat ctaaattggt ttctttttta
     5521 tatccatttt ggtaaataat agatactctt tttagccaaa tattgttgga gcgagaccaa
     5581 gaatatgtca ttgcttcttc aaggtttcta tattttaaga aaaaaggatt tacaagttta
     5641 ctacttatta aatcaacact atcccaccac gatttattta aaattaaact ttcaaaaaac
     5701 acaatgtctg ttggcaccca tttttttttg tattttatta acaaatgtat ggcaaaataa
     5761 tgatattccc tttctggcaa gttaaaaagt tctctaacaa tagcttttgt ttcatcatag
     5821 tcgggaatag gaaaagtgtt gaaaaataaa ttaaaaagtt tatttcgttt agaagccggt
     5881 attccaataa atggaaattt atacctcatg tatttagaca ttggttcagc ctcttcttcg
     5941 tttctgtact tttcaaaaaa ttcttgaatt tttttcacat aatccgataa taacatattg
     6001 atataacttt ttatgttgtg caaagttaag aaaaaacagg ttaatatcaa aacgcttttg
     6061 ttaaattagc taataatttt taaataaatt atatcaacat tttgttttta tacttaaatg
     6121 ttatattttt atcattttaa actcatgaga gaatttgtga aaatattgaa aattttatgt
     6181 ttatttcctt taatgttgat ttataggagt gtggtaacat ttagaaattt tttatatgat
     6241 tctaaaatat taaaatcaca tgagtttgat atgccaataa tttctgttgg aaatatttct
     6301 gttggcggtt ctggaaaaac accacacaca gagtttttga tagaaatgtt gcaagacgaa
     6361 tatcaagtag ctgttttaag taggggttat aaacgaaaaa ctcgaggttt tagattagta
     6421 gaagcagacg actcattttt attaagtggt gatgagcctt tacaaataaa gcaaaaattt
     6481 ccaaatgcta ttgttgctgt ttgtgaaaat agggttaagg ggatttcgca attaatgtca
     6541 aaatttcctg acttaaaatt aataatttta gacgatgctt ttcaacatag aagagtaaag
     6601 cctggtttgt caattttgct aaataatttt aatcacccta taaataaaga ctttttgttg
     6661 cctttaggtt atttgagaga gccacgaagc tctgcatatc gagcacatat agtagttttt
     6721 acaaaatgtc ctaataaatt aaaaccaatt gaaaagcgta tattaattaa agaatcagag
     6781 gtttatcctt atcaatattt gttttttact acatacaatt atttagattt gaaacctgtt
     6841 tttaacgaag ataaaaaaac tatacccatt tctgatttga aaaaatataa tgtggtttta
     6901 ttttctggac ttgctaagca cacaagttta tataattttt ttaaagaaaa ctcaaaaagt
     6961 gtaaaacata ttaagtttcc cgaccatcat aattttaaaa aacgagatat aataaaaatt
     7021 gagaaggaat ttaaaaacac aaaagaacca agcataattg taacaacaga aaaagatgcg
     7081 gtaaaactta aagataataa atattttgca gaagaaacaa aaaaaatgtt atattgcgcc
     7141 ccaataaaag tggaacaatt gggaagcgaa gaagatatta aacaatttaa taatcaaatt
     7201 tttagttatg ttagaaataa taaaagatac tgtaagttat ataaaaacgc atattcaggt
     7261 tgagcctgaa ataggaatag ttttaggaac tggcttaggc ggattagtaa aagaaataga
     7321 tattcatacg agtttgagtt atgaaaaaat accaaacttt cctatttcta ccgttgaagg
     7381 acatagtgga aaattaatat ttggaaaatt aggtggcaaa aatgttgttg ctatgcaagg
     7441 acgtttccat tattacgaag gatacgatat gaaccaagtg gttttcccta ttagagtgat
     7501 gaaatatttg ggaataaaaa ctttgttttt atcgaatgct agtggtggag taaatcctga
     7561 ttttgaaata ggagatttaa tgataattac cgaccatatt tgtttggtgc caaatccact
     7621 aattggtaaa aatgagccag agtttggaac tagatttcca gatatgagcg aagcttatga
     7681 caaaaactta attgcaaaag caaaacaagt agctcaaaat ttagggataa aagtaactca
     7741 aggagtttat acagcaactt ctggtccaac ttttgaaact ccatcagaat ataaatattt
     7801 tagaataata gggtctgata cagttggaat gtcaacagca ccagaagtta ttaccgctcg
     7861 tcatttagga ttgccatgtt ttgcaatgtc gataattaca gatttgggtg ttcccggaaa
     7921 aattgttgaa gttacccacg aagaagtgca aaaagtagca gctgctgcag aatcgaagat
     7981 gacacaaata tttaaacaaa tgatagcaga aatttaattg aaaaattata ttcaatcatt
     8041 tttttctgac ttctaaaaaa ctttgtttta tatttgcata aaatggaaaa tgatatttta
     8101 catataaatt caaagtttta ttgggcttct ctgatattga taattcttgt tttatcattt
     8161 tcctgctcgt caacaaaata cgtagctgaa ggagattatt tgctagctag tacaaaaatt
     8221 aacatcgagg ggtcttcaga tattgataaa gaagatttag aaagttcgtt acgacaaaaa
     8281 ccaaacaaaa aggtttttgg tctcagattt tatttaatgg tttataatag tataaatcaa
     8341 gataaagaaa aacttagaga agaaaaacgt aaagtaaaag atgctgaaag aaatgcaaaa
     8401 agaaaagcaa agggcaaaaa accaaaagat agattttata tcactcgttg gctttctaat
     8461 ct
//
LOCUS       DAOPNX010000103         8314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_9582_length_8314_cov_8.185858, whole genome shotgun sequence.
ACCESSION   DAOPNX010000103 DAOPNX010000000
VERSION     DAOPNX010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8314
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9582_length_8314_cov_8.185858"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(593..4006)
                     /locus_tag="PKN53_09660"
     CDS             complement(593..4006)
                     /locus_tag="PKN53_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89254.1"
                     /translation="MFSQSLINLRNKEISLDQDTIKLDSLSIVSQSLNLTDCDGNKIP
                     SDSFDFDFKNSSIIFKQKPNCDKINISYKTFPVNYFEPKTIFKKEDFLISAKDDGFNN
                     YDYVFSFEDFKSANQKYNLSRKGSISRGVGFGNNQDVIVNSAMNLQLDGQISDNLFIQ
                     AAISDNNIPIQPDGNTQQLQEFDKIFIKIFNDKFSLTAGDFELSRPKGYFLNYNKKAQ
                     GAKINFNTDIETKSGKKISFNNQISGAASKGKFNRQEIIGIEGNQGPYKLVGANNEQY
                     IVVLAGTERIYIDGKLLNRGFENDYTIDYNLGEITFTTKQPINKDKRIIAEFEYSDRN
                     YSRFLITGSTEITYEKSKFWLNFYDESDNKNQPFDQNITQQDRFLLSQIGDNLDMALI
                     PGFDSLEFVPSEVRYKLCDTIVEGFVYDSIFVYSSDPELAHYRVSFAFVGENNGFYKK
                     GVSAANGRVYEWVAPENGKPQGDYMPYRLIVTPKKSQLINAGGELNLGEKTQINFEAA
                     FSKNDVNTFSKLDSHDDNGLALKTGLKQAFYKTEKTWFGAFADYMFLQKHFRGIENFR
                     STEFTRDWNLNNMYSYNNENNISGGILYSDKKLGTANFTSTYLDRGVIYRGLQNLLNT
                     NLKLKSWSIIGNASLLQNKDSLTNAEFLRHNLRIKKDFKFLSLGLGEFAENNTRNMLT
                     DNSLALNSFKFNSYEAFLNSLDSAKVQFNLNYKYREDFLPKNNELNLSSNSHDFNLGT
                     SLEQNPNHKFKLELNYRILDVNDTSLYRGNPENNTTGRAEYNFRLWKGAISSGNFYEI
                     GSGLERKTEFAYVEVAPGQGVYTWTDYNNNGIQELDEFEIANFIDQANFIRIINPTAE
                     YIKTYSNQISQNVNFNPSVLLRNKKGFLKFLSKFSNQTAYMISQKNTSSEFLEYANPF
                     AQNIDDINLVNQTSSFRNSLSFNRGNPKIGADYIINSNNNKLLLVSGFDTRKTFSHTL
                     QMRYNINSKLGFQNKVSQTDKAYNSEYFSSKNYEINILNNEFQFSFQPNLSNRVSIKH
                     IFKQKDNIEGEKLLSNDFGLEYRLSSVKKGSLTANFNYIHYKYNASTNGALAYEMLEG
                     FMPGSNLTWGLAFQRQLANGLQININYNARKSPDSKIIHNANVQVRAFF"
     gene            complement(4330..6489)
                     /gene="pnp"
                     /locus_tag="PKN53_09665"
     CDS             complement(4330..6489)
                     /gene="pnp"
                     /locus_tag="PKN53_09665"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017141361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOD89255.1"
                     /translation="MYNEIKQVVKLDDGREIEISTGKLAKQASGSVVVKMGKTMLLAT
                     VVVADDAKEDVDFMPLSVEYKEKYAAVGRFPGGFNKREGRASDYEILVSRLVDRALRP
                     LFPDDFHAEVFVNVMLISADKNDLPDALAGLAASAAMSVTDIPFNGPISEVRVARIDG
                     NFVINPTAEDNKRADIDLIVAATIDNIMMVEGEMNEVSEKEMLEAIKFAHEAIKVQCQ
                     AQIELRKACGIEGFAEYCHENNDEDLRKKVWDETYDKAYAAAMTQTADKHERLRLFSE
                     VKENFCAQYDEESLEKIQTLIDRYYHDVEKEAVRQMVLNEKVRLDGRKTNEIRPIWSE
                     VDYLPAAHGSAIFTRGETQSLTTVTLGNKIDEQQIDGAMIAGYEAFILHYNFPPFSTG
                     EARPYRGVGRREVGHGNLAHRALAKVVPEKGEKFPYTVRVVSDILESNGSSSMATVCA
                     GCLALMDAGVPISKPVSGIAMGLIADPKTGKFAILSDILGDEDHLGDMDFKVCGTKDG
                     ITATQMDIKVDGLSYEVLEQALEQARQGREHILNEMSKTLAQPRPEYKEHAPRIETIV
                     IPKDMIGAVIGPGGKIIQEIQALSNTVIGIEEKDDKGYVEISANNQTDMNLALSKVKA
                     IVAVPELNEIYTGKVKGITTFGAFVEILPNHQGLLHISEIDWKRIKSVEEVLKEGQEI
                     EVKLIEIDQKTGKFKLSRKALLPRPPKEERPEQKSEK"
     gene            complement(6557..6832)
                     /gene="rpsO"
                     /locus_tag="PKN53_09670"
     CDS             complement(6557..6832)
                     /gene="rpsO"
                     /locus_tag="PKN53_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOD89256.1"
                     /translation="MKNYLDSDKKKEFVEKHGKSNKDTGSPEVQIALFSYRISHLTEH
                     LKIHKKDFSTQRALISLVGKRRALLDYLKDKDIERYRAIVKELGLRR"
     gene            7006..7368
                     /locus_tag="PKN53_09675"
     CDS             7006..7368
                     /locus_tag="PKN53_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89257.1"
                     /translation="MKYLRHKIIRIFLLSFFGLYIVGNSLFTHSHEINGVKFTHSHFF
                     NLLGTEGQTTKTHEHSEKEILTIQILNHAFLACAFVFAIGFVNKLFYQKIKLGFFTPT
                     YYHLFLLDTKNLRAPPIL"
     gene            7469..>8314
                     /locus_tag="PKN53_09680"
     CDS             7469..>8314
                     /locus_tag="PKN53_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOD89258.1"
                     /translation="MSFLSAFSQNKTDINIFGHVTDSFTGEHVPYINIQLKGTTIGTA
                     TDATGHFYLKNLPKGEFSIVASGLGYKNSEQTVKLELGKSIEINFVIEEDKVLLETVV
                     VSANRNEVNRKEAPNIVNVISPVSLENTSSNNLAQALVFQPGLRVENNCQNCGFQQVR
                     INGLDGPYSQILVDSRPIFSSLAGVYGLEQIPANMIDRVEIVRGGGSAIFGSNAIAGT
                     INIITKEAINNSVTVANTANLIGGRSLDNFTSLNASLVSDSYKTGIVIFGSNRQQSPY
                     DHDGDG"
BASE COUNT         2705 a         1343 c         1263 g         3003 t
ORIGIN      
        1 attaaattaa atccaaaatt cttaattccc aataattgtc atactaaaaa ctaaatcatt
       61 cataatcaac ttgcaaagat attatgtttt tttttaatgg agtgcttttt ttgtgttttt
      121 tttatataaa aatgacaatt ttttttaacg gactttttta tgctcaattt tagatttgca
      181 tagataagat acaaaacagt taaaaggtaa aagcgatgca ctgaacgtag ttgaagtgtt
      241 aaaagctgtt cggcttttct ttatgaactt ttaactgata aaccttgctc gctcggattt
      301 ttaatccgag tgtaaactac caacactcgg attaaaaatc cgagcgagca tcatgggtat
      361 ttggagcgag catcagggtg ttaaaagcga atactagagt gttaagattg tgagcagagt
      421 gagcttgcga actctgcgaa gcaatagacg cgtcagccct ttcaactttt aattcattct
      481 taattatcgg gtgttgagcg aagccgagac gggtgttgag cgaagtcgaa cgggtgttga
      541 gcgaagtcga acgggtgttg agcgaagtcg aaacacaatt cttaattctt aattagaaaa
      601 aagccctcac ttgcacatta gcgttatgaa taatttttga gtcgggagat tttctggcgt
      661 tgtagttaat atttatttgt aatccatttg ctaattgcct ttgaaaagct aagccccaag
      721 ttaagtttga accaggcata aatccttcta acatttcata ggctaaagct ccgttggtgg
      781 aagcattgta tttgtagtgt atataattaa aattggctgt taaacttcct tttttcacag
      841 aacttagtct atattctaaa ccaaaatcgt ttgataaaag tttttcgcct tcaatattat
      901 ctttttgttt gaaaatatgt ttaatactta ctctattact taaatttggc tgaaaactaa
      961 actgaaattc attgtttaaa atattaattt catagttttt tgaagaaaaa tattctgaat
     1021 tataagcctt atcggtttga gaaactttat tttgaaatcc aagttttgaa ttaatattat
     1081 aacgcatttg taaagtatga gaaaatgtct ttctagtatc aaagcccgaa actaacaaaa
     1141 gtttattatt gtttgaattt ataatataat cagcacctat ttttgggtta cctctgttaa
     1201 aagacaaaga atttctaaac gatgaggttt gattaacaag gtttatatca tcaatatttt
     1261 gagcaaaagg attagcatat tctaaaaatt cggaagaagt atttttttgt gaaatcatat
     1321 aagcagtttg gttagaaaat ttagacaaaa actttaaaaa accttttttg ttccttaaca
     1381 aaaccgaagg attaaaattt acattttgac tgatctgatt agagtaggtt ttaatatatt
     1441 ctgccgttgg attaataatt ctaataaaat ttgcttggtc aataaagttt gctatttcaa
     1501 actcatcaag ttcttgaatg ccgttgttgt tataatctgt ccaagtataa actccttgtc
     1561 ctggagctac ttccacatag gcaaattcgg tttttctttc taaacccgaa ccaatttcat
     1621 aaaaatttcc tgaagatatt gcacctttcc acaatctgaa attatattct gctcttcctg
     1681 ttgtattatt ttcaggattt cctctgtaaa gcgaagtatc gttaacgtct aaaattctgt
     1741 aattcaactc taatttgaat ttatgatttg gattttgctc taaagaagta ccaaggttaa
     1801 aatcgtgaga attgctcgac aaatttaatt cattattttt cggcaaaaaa tcttctctat
     1861 atttgtaatt aagattgaac tgaacttttg cactatctaa actatttaaa aatgcttcgt
     1921 aagagttaaa tttaaaacta ttcaaagcca agctattgtc tgtgagcatg ttgcgagtgt
     1981 tgttttctgc aaattcacct aaaccaaggc ttaaaaattt aaaatctttt ttaattctta
     2041 aattatgtct taaaaactca gcgtttgtaa gcgaatcttt attttgtagc aaagaagcat
     2101 ttccaattat tgaccaagat tttaatttta aatttgtatt gagcaagttt tgtaaacctc
     2161 tgtaaatcac gcctctatct aaataagttg atgtaaaatt agcagttcct aattttttgt
     2221 ctgaatataa aattcctcct gaaatattat tttcattatt atatgaatac atattattca
     2281 aattccaatc gcgagtaaat tctgtgcttc taaaattttc tattcctcta aaatgctttt
     2341 gtaaaaacat ataatcagca aaagctccaa accaagtttt ttcagttttg taaaaagctt
     2401 gttttagtcc tgtttttaga gcaagtccat tatcatcatg tgagtctaat tttgaaaaag
     2461 tgttcacatc gtttttgctg aaagctgctt caaaatttat ttgcgttttt tcgcctaaat
     2521 taagttctcc accagcattg attagttgag atttttttgg tgtaacaatc aaacgataag
     2581 gcatgtagtc tccttgtggc ttgccatttt ctggagcaac ccattcataa actctaccgt
     2641 tagcagccga aacgcctttt ttataaaaac cattattttc gccaacaaaa gcaaaactta
     2701 ctctatagtg agccagttca gggtctgagc tatatacaaa aatggaatca taaacaaatc
     2761 cttcaacaat ggtgtcgcag agtttgtagc gaacttcaga aggaacaaat tccaaagaat
     2821 caaatcctgg aattaaagcc atatcaagat tatcgccaat ttgtgaaagc agaaatctat
     2881 cttgctgtgt aatattttgg tcgaaaggtt gatttttatt atcgctttcg tcgtaaaaat
     2941 ttaaccaaaa ttttgatttt tcgtaagtga tttctgttga acctgttatc aaaaatcttg
     3001 aataatttct atctgaatat tcaaactctg caataattct tttgtctttg ttgataggct
     3061 gttttgttgt aaaagtaatt tctccaagat tataatctat agtataatca ttttcaaatc
     3121 cacgatttaa aagcttgcca tcaatgtaaa ttcgctctgt gccagccaat acaacaatat
     3181 attgttcgtt gtttgcacca acaagtttat aaggtccttg attgccttct attccaataa
     3241 tttcttgacg attaaatttt cctttgcttg cagcaccact aatttgattg ttaaaactta
     3301 ttttttttcc agattttgtt tctatatcgg tattgaaatt aatttttgct ccttgtgctt
     3361 ttttattata atttaaaaaa taaccttttg gtctgctcaa ctcaaaatca ccagcagtaa
     3421 gtgaaaattt atcattgaaa atttttataa aaattttatc aaactcttga agttgctgag
     3481 tgttaccatc aggttgaata ggaatattat tatcgctaat tgctgcttga ataaataaat
     3541 tatcgctaat ttgtccgtct aattgcaagt tcatggcaga atttacaata acatcttgat
     3601 tgtttccaaa cccaacacca cgactaatac tgcctttgcg tgacaaatta tacttttgat
     3661 ttgctgattt aaaatcttca aaactaaaaa cataatcata gttattaaag ccgtcatctt
     3721 ttgcactaat cagaaaatct tcttttttaa aaattgtttt tggctcaaaa tagttaacag
     3781 gaaaagtttt gtatgaaata ttaattttat cgcagttagg tttctgtttg aaaattatgg
     3841 aagaattttt aaaatcaaaa tcaaaagaat ctgaaggaat tttattgcca tcgcaatctg
     3901 ttaaatttaa actttgtgaa actatactaa ggctgtctaa cttaatagtg tcttggtcta
     3961 acgaaatttc tttatttcgt aaattgatta atgattgtga aaacataaat aaatttatgc
     4021 acaaaaataa tattatgagg gcatactttt tcacaatttt tttttacaaa aataagtata
     4081 gatgtttata acgacaaaaa aactaaaaaa ttggaaattt tgtgaaaaaa taaatataca
     4141 gttaaaagtt aaaagtcgaa agtagtctgc gggtttggca tttcttagca aactttaaac
     4201 ttgataaact tttaactgaa ttagggtggt agaataaatt aaaatttgta tagataagag
     4261 acggcgcatg caccgtctct acactatact gcataattta ttcaaaattc gacttttaac
     4321 ggataaaatt tatttttcag atttttgctc aggtctttct tcctttggag gacgaggcaa
     4381 caaagcctta cgacttaatt tgaattttcc agttttttgg tcaatttcaa ttaattttac
     4441 ctcaatttct tgtccttctt ttagtacttc ttcaacagat ttaattcgtt tccaatcaat
     4501 ttcggaaata tgtaataaac cttgatgatt tggtaaaatt tcgacaaaag ctccaaaagt
     4561 agtaatacct tttactttac cagtataaat ttcgtttaat tctggaacag caactatagc
     4621 ttttacttta ctcaaagcta agttcatgtc tgtttggtta ttagcagaaa tttcaacata
     4681 acctttgtcg tctttttctt caattccaat tacagtattt gataaggctt gaatttcttg
     4741 aataattttt ccaccaggac ctataacagc accaatcata tctttaggaa ttactatagt
     4801 ttcaattctt ggagcatgtt ccttatattc aggacgaggt tgtgccaagg tcttagacat
     4861 ttcgtttaaa atgtgttctc tgccttgtct tgcttgttct aaagcttgtt ctaaaacttc
     4921 ataagaaagt ccatcaactt taatatccat ttgagtagcg gtaattccgt ctttagttcc
     4981 gcaaacttta aagtccatat caccaagatg gtcttcgtcg ccaagaatat cacttaaaat
     5041 agcaaatttg ccagtttttg gatcagcaat taatcccatt gcaattccgc ttacaggttt
     5101 agaaattgga acaccagcat ccataagagc aagacaacca gcacaaacag tagccattga
     5161 tgaagaacca tttgattcta aaatatcaga aactacgcgt actgtataag ggaatttttc
     5221 gcctttttca ggaacaactt ttgccaaagc tctgtgagca aggttaccat gaccaacttc
     5281 tctacgacca acacctctgt aaggtctggc ttcaccagta gaaaatggag ggaaattata
     5341 atgtaatata aaagcttcgt atcctgctat cattgcacca tcaatttgtt gctcgtctat
     5401 tttatttcct aaagttactg tagttagaga ttgagtttcg ccacgagtaa aaatagcaga
     5461 accatgagct gcaggtaaat aatcaacttc gctccaaata ggacgaattt cgttagtttt
     5521 acgaccatca agtcttactt tttcgttgag taccatttgg cgaacagctt ctttctcaac
     5581 atcatggtaa tagcggtcga taagtgtttg aattttttct aaactttctt catcatattg
     5641 agcacagaaa ttttctttta cttcagaaaa taatcttaaa cgctcgtgtt tgtcagcagt
     5701 ttgagtcatc gcagcagcat aagctttgtc ataagtttcg tcccaaactt tttttcttaa
     5761 atcttcatca ttgttttcat gacaatattc agcaaaacct tctattccac aagctttgcg
     5821 aagctcaatt tgtgcttgac actgaacttt aatagcttcg tgagcaaatt ttatagcttc
     5881 caacatttct ttttcagaaa cttcgttcat ttcgccttca accatcataa tattgtcgat
     5941 agttgcagca acaatcaagt caatatcagc tcgtttgtta tcttccgcag ttgggttgat
     6001 tacaaaattg ccatcaattc ttgcaactct tacttcagaa ataggaccat taaaaggtat
     6061 gtctgttacc gacatagcag ccgatgcagc caagccagct aatgcatcag gtaggtcgtt
     6121 tttatcagcc gaaataagca ttacatttac aaaaacttcg gcatgaaaat catcagggaa
     6181 aagtggacgc aaagctctgt caactaatct tgatacaaga atttcgtagt cagaagctcg
     6241 cccttctctt ttgttgaagc ctccaggaaa acgaccaaca gcggcatatt tttccttgta
     6301 ctcaacagaa agaggcataa aatcaacatc ttctttcgca tcatcagcaa caacaacagt
     6361 tgccaagagc atggttttac ccatcttcac cactaccgaa ccacttgctt gcttcgctag
     6421 tttaccggtg gaaatttcga tttctcgacc gtcgtctaat ttaacgactt gtttaatttc
     6481 attatacata tatttcaatt caaatttatt ttaaaaacaa aaaaagcaaa acttagtgtt
     6541 ttgctttctt agcggtttaa cgacgtaaac ctaattcttt tactattgct ctatatcttt
     6601 cgatatcctt gtcttttaaa taatcaagca aggctctacg ttttccaacc aatgatatca
     6661 aggcacgttg agtactaaaa tcttttttgt gaatttttaa gtgctcggtt aagtgactga
     6721 tacggtagga aaataaagca atctgaactt ctggagaacc agtgtcttta ttagactttc
     6781 cgtgtttttc gacaaattct ttctttttgt ctgaatctaa ataattcttc atcacttttt
     6841 atttttgttt ataaattaag ggtgcaaaaa tactacttct tttgatatgt gcaaaattat
     6901 tttatagaaa attctattcc tgaaaacaaa aaatctgcta aaaaacactt aactattatt
     6961 tggaattatt ttaaataaga ttacttttgc cacaaaattt taaaagtgaa atatttaaga
     7021 cataaaatta taagaatatt tttactaagt ttttttgggt tatatattgt aggaaactcc
     7081 ttatttactc atagtcacga aattaatgga gttaaattta ctcactctca cttttttaat
     7141 ttattaggta cagaaggtca aacaactaaa actcacgaac atagcgagaa agaaatttta
     7201 actattcaaa ttttaaatca tgcttttctt gcttgtgctt tcgtatttgc aataggtttt
     7261 gtaaataaat tattttatca aaaaataaaa cttgggtttt tcactccaac ttattatcat
     7321 ttatttttac ttgatacaaa aaacttaaga gccccaccta ttttataatt aatagaggat
     7381 tttgtggtgt taaactgaaa ttcagtaagg gtttaatata aaatttattt ttatgagaag
     7441 ttttttgtta attacaataa tatttttgat gagtttttta tctgcttttt ctcaaaataa
     7501 aacagatata aatatttttg gtcatgttac agatagtttt actggcgaac atgttcctta
     7561 tataaatatt caacttaaag gtacaacaat cggtacagca acagatgcaa caggtcattt
     7621 ttatttaaaa aatttgccta aaggagaatt ttcaatagtt gcttcaggac ttggttataa
     7681 aaattcggaa caaacagtta agttagaatt aggaaaatca atagaaataa actttgttat
     7741 agaagaagat aaggttttat tagaaactgt tgttgtttca gcaaatcgta atgaagtaaa
     7801 tcgaaaagaa gcacctaata tagtaaatgt tattagtcct gtttcattgg aaaatacaag
     7861 ttctaataat ttggctcaag ctttggtttt tcagccagga ttaagagttg aaaataattg
     7921 tcagaattgt ggttttcagc aagttagaat aaatggactt gatggacctt attctcaaat
     7981 tttagttgat agtcgtccaa tttttagctc tttagctggc gtttatggtt tagagcaaat
     8041 tcctgcaaat atgatagata gagttgaaat tgttagggga ggaggttctg caatttttgg
     8101 ctcaaatgct attgctggta ctattaatat aattactaaa gaagctataa acaattctgt
     8161 tacagttgca aatacagcca atttaattgg tggacgttca cttgataatt ttacaagttt
     8221 aaatgcttct ttggtttcgg attcttacaa aacaggaata gtgatttttg gttcaaatcg
     8281 tcagcaaagt ccatatgacc acgatggaga tggc
//
LOCUS       DAOPNX010000104         8170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_9804_length_8170_cov_6.842637, whole genome shotgun sequence.
ACCESSION   DAOPNX010000104 DAOPNX010000000
VERSION     DAOPNX010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8170
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9804_length_8170_cov_6.842637"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(412..6489)
                     /locus_tag="PKN53_09685"
     CDS             complement(412..6489)
                     /locus_tag="PKN53_09685"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOD89259.1"
                     /translation="MKSLIQKFAFFLSITLIAVIFSIAQNNINKKDKYESLWKEVEEF
                     QKNSLPKSALEKVEKIYALALSENEDVQIIKSLIYITQYTAILESEGNDYEKAISKLE
                     IETAKSSGAVKPMLHLLLAKMYSQYYSNNSYLINQRSVTYNFDLNDMQTWDKTRFQDK
                     IIKNYLLAIDKSLNDIPIKDYKEIIINAENSMPKFETLLDFVAHSAIKGLTSTGQYRR
                     YYYYDNSAQNEDKFTNNSFFNDAEDFTKIKFDYDSLSFNYSVLKIYQDWLEVKLNKND
                     NLASLLMVDIDRLDFVLRNFNSIEKEKLWLTTVENLHKKYSKNPESTLTAYKIANYYS
                     NLASQYDFKNPTTAKYKDYNKTAIALLDEALKLYPDALYSAECENLKNTIEKFNLSFT
                     SEKIVSSEDNFPLRISYKNTDKIYLTILSLDYNTYLTLERKYYNKELYEKLIKSSKTV
                     VNSKEIDLPKTDDYNSHYTDYLVDGLSKGFYYIILHAEPELNFEKNYISTNSIFVSDL
                     ALTELDDYSTSKIYVLNRKTGSPVKGAKVEMYKNEYSHIKRDYVDKLVSTKFSDENGE
                     ITLNISEKNSYSNLRFDVSKNDDYLSESFYVYSRGKHEEKSQIEIKFFIDRGIYRPGQ
                     TVYYKGICIEKKGEEAKILPNYSTSLFVRDVNHQNIHQTTVKTNEYGSFNGSFTIPLD
                     VLAGNFSIQTNDGSISFKVEEYKRPNFEVKMLPVEGEYRVNDSIKAQGKATTYAGTAL
                     TDAKVTYSVVRNPIWRRYSPYSFSSKEIAFGETKLDDNGKFEINFKAIPEDESTINEY
                     IYYNYTIKVFVTDINGETQQTSGYVYVSNKALSISHNLSGIIEKANLDSINIKTTNIQ
                     GVSVPAKVDIEIFKLKDPKLLLAKRMLDEVDNKLYSKEEWYSKNPGYEYEAETKFENW
                     ETEKSVFKSNINTENTNKIQLNGSDKWEAGVYRIVLKSKDKWNNEISDVQNIVVYSSS
                     SKQMPYTATDFFQVDKNTAFVGETVNIYLGSSYKNVEVFYTLTKKGENQQVQRLNINS
                     EVKKIEIPIDESYLGGLGINLMFIKNNRLYSFSENISVPWENKKLSIKFTSFRDKTLP
                     GSNEEWTLKITDKSDKPAEAEFLASMYDASLDAFRSNSWYWSIFPSYHYYSNWSNNCF
                     SSSNSSNNDYNYFKQYKYTYWYEPELNLFNVNRYSYYSGGGRRDAKYSSPAIMADANQ
                     VMEESALGDVEVVQDVVIKSLPQDSEAPIADQAEKKVQIRSNFNETAFFYPNLVTDAD
                     GNINLKFTVPESLTRWNFLGFAHTKDLKLGSITEKMVCQKELMIMPNYPRFFREGDKI
                     ELNAKINNVSEKDINGKAKIEIYDPVSLKVLNSEFLKNNNNELDFSVKAGGNTTVLWN
                     LAISDKYSMIGVRIIAEGLEHSDGEERIFPVLSNRMLVTETKPLPVRKAGITKFSFAS
                     MKNNNSQTLKHHSYTLEFTSNPAWYAVQALPYLMEYPRECSEQVFSRFYANSLASHIA
                     NSNPKIKRVFDTWRNTKDSNMLYSNLEKNQELKALLLEETPWVLQAQDESTRKKQVAL
                     LFDLNKMSYEKDAALKKLLKQQKYNGGWSWFDGMPESWYITQHIVAGLGHLDKLGVND
                     VKNDNKTWGMTKKAIDFIDGELARSYKEMKKYCDKECMKNDHLGYMDIHYLYARSYFI
                     NEIPIPQATKEAFNYYIEQSEKYWTNKNFYMQGMIALALNRYEKTATPKKIIASLKEH
                     AQHDEEMGMYWKYGRAYYWYQAPIETQALLIEVFDEVANDLESVDEMKVWLLKQKQTQ
                     DWKTSKATVEAIYALLLRGTDILADDEIAKITIGSMNIDAANDPEIKTDAGTGYFKKA
                     WDGSQIQKDWADISVEKSNNTVSWGAVYWQYFEQLDNIKSFDETALKIDKKLFVERRL
                     GGKSVIVPIEKDNKLNLGDKIKVRIEIRVDRDMEYVHLKDMRASCFEPVDYISGYRYK
                     AGLGYYQEIKDAAMNFFIDYLRKGTYVFEYDLIVAQKGDFSNGITSIQSMYAPEFSSH
                     SEGVRVVVK"
     gene            6609..7778
                     /locus_tag="PKN53_09690"
     CDS             6609..7778
                     /locus_tag="PKN53_09690"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD89260.1"
                     /translation="MKNFYSILFLCLVSTFIFGQTQTTRELVWDSQTRQYIEYIPTAY
                     NSENPTPVMFCLHGLGDDMQNFHNSLELNQWGETANWIIITPQALDAQIPIMGSLGNA
                     WNSGAGAEGIPILGTVILNDNVDDAGFLMAILDSLENNLNINTDSVFFMGFSLGGFMS
                     NRMAIEHGDRITAIASVSGTIGMAVKDQIPVAKVNTLHFHGTNDETIAYEDAGFPIGG
                     TNYSTGLGAEQTVDYWKTHNQCGNEATHTFFPDEKEDGLTFERFLYKNESENIQTALI
                     KANNGIHDWYYTPVNDIHYSIEIYKFFTNTMDFPTDIKNTTIESLNIYPNPASDILFV
                     SQDVDEIAIFDVTGKLVLKEFNNNSINISALNSGLYIVKTISEGKNYEHKIQIIK"
BASE COUNT         2582 a         1259 c         1208 g         3121 t
ORIGIN      
        1 aagtggcgca actttattaa ttaaaagagt ttttagtgtt gagtttttag ttttttagta
       61 tgaagataat taagaattaa gaatgaataa ttaagaatta tatgctcgct ttgctcgctt
      121 gggtttgctt cgcaggttca caggctcact cttattagtt tttagtgttg agtttttagt
      181 ttttagtagt gagtttttaa aaacttcaca ctgataaaat tcctttagtg catagtttgt
      241 atatacttgc ttcgcagagt tcgcaggctc actctgctcg caattacatt acccatgacg
      301 ctcgctcaaa cacccatgac gctcgctcgg atttaacttc gttgagctcg tcctgcggac
      361 ttcggttgta atccgagtgc atggaattgt ttctccgcgc gagcgggaaa tttatttcac
      421 caccaccctc acaccttcgg agtggcttga aaattcaggt gcgtacatgg attgtatgct
      481 ggtaattcca tttgagaagt cgcctttttg agcaacaatt aaatcgtact caaatacata
      541 agttcctttt cttaggtagt ctataaaaaa gttcatagca gcatctttaa tttcttgata
      601 ataacccaag ccagctttgt agcgatagcc cgatatataa tcaaccggct caaaacaaga
      661 tgctcgcata tcttttaaat gaacgtattc catatctcta tcaacgcgaa tttctatacg
      721 tacttttatt ttgtcgccga gatttagctt attgtctttt tcaattggaa ctataacaga
      781 tttgccacca agtctgcgtt caacaaaaag ttttttatcg attttcaatg ctgtttcatc
      841 aaaactttta atattatcaa gttgttcaaa atattgccaa taaaccgctc cccacgaaac
      901 agtattattg cttttttcaa cgctaatatc agcccaatct ttttgaattt gcgaaccgtc
      961 ccatgctttt ttgaaatagc cagttccagc atcggtttta atttctgggt cattagcagc
     1021 atcaatattc atagagccaa tagtaatttt tgcaatttca tcatcagcta aaatatctgt
     1081 tccgcgtagt aatagtgcat aaattgcttc aacagtagct tttgatgttt tccaatcttg
     1141 agtttgcttt tgttttaaca accaaacttt catttcgtca acagactcta aatcgttagc
     1201 aacttcatca aaaacttcga ttaaaagtgc ttgagtttct attggtgctt gataccagta
     1261 ataagctcta ccgtatttcc aatacattcc catttcttcg tcgtgttgag catgttcttt
     1321 aagagaagct ataatttttt taggagtagc tgttttttca taacgattta gtgcaagagc
     1381 aatcattcct tgcatataaa agtttttgtt agtccaatat ttttctgatt gttcaatata
     1441 ataattaaaa gcttcttttg ttgcttgtgg aataggaatt tcgttaataa aatatgatct
     1501 tgcatataaa taatgaatgt ccatataacc taaatggtca tttttcatac attctttgtc
     1561 gcaatatttt ttcatttctt tgtatgacct agcaagttcg ccgtctataa aatctatggc
     1621 ttttttggtc attccccaag ttttgttatc atttttcaca tcgttaactc ctaatttatc
     1681 taaatgacct aatccagcaa caatatgttg agttatgtac caactttcgg gcattccatc
     1741 aaaccaagac cagcctccat tgtatttttg ttgtttaagt agttttttta gagcagcatc
     1801 tttttcataa ctcattttgt ttaaatcgaa aagcagagca acttgttttt ttcgagtgct
     1861 ttcgtcttgt gcttgtaaaa cccaaggagt ttcttcaagt aacaaggctt ttaattcttg
     1921 atttttttct aaattagaat aaagcatatt gctatcttta gtatttctcc atgtgtcgaa
     1981 aactctttta atttttggat tgctattagc tatgtgagaa gctaaagaat ttgcataaaa
     2041 tcttgaaaaa acttgctctg aacattcgcg aggatattcc attaagtacg gtaaagcctg
     2101 aactgcatac caagcaggat ttgaagtaaa ctcaagagta tatgaatgat gttttaaagt
     2161 ttgtgaatta ttatttttca tgctagcaaa gctaaacttt gtaatgccag cttttcgtac
     2221 tggtaaaggc ttggtttctg taacaagcat tctgttgcta agaacaggga aaattctttc
     2281 ttcaccgtca gaatgctcaa gtccttcggc aataatgcga acgccaatca tgctgtactt
     2341 gtcgcttata gcaagattcc acaaaacagt ggtatttcca ccagctttta cagaaaaatc
     2401 aagttcatta ttattgtttt tcaaaaattc tgaatttaaa acttttaaac ttacagggtc
     2461 gtaaatttct attttagctt ttccatttat atctttttct gagacattat taatttttgc
     2521 gtttaattca attttgtcgc cttctctaaa aaagcgagga tagtttggca ttatcattaa
     2581 ttctttttgg caaaccattt tttctgtaat gcttccaagt tttaagtctt ttgtatgtgc
     2641 aaaaccaaga aaattccatc ttgtaagact ttcgggaaca gtaaatttaa ggtttatatt
     2701 tccatctgca tcagtaacta aatttggata gaaaaatgca gtttcgttaa aattacttcg
     2761 tatttgaacc tttttttctg cttggtctgc aattggagct tcgctatctt gtggaagtga
     2821 tttaattacc acatcttgta caacttcaac atctcctaat gcagattctt ccattacttg
     2881 gtttgcatct gccataattg ctggtgaact atattttgca tctcttcttc ctccaccaga
     2941 ataataagaa tagcgattta cattaaataa attaagttct ggctcatacc aatatgtata
     3001 tttatattgt ttgaaataat tataatcgtt gtttgaagaa tttgaagaac taaagcagtt
     3061 attagaccaa tttgagtaat aatgataact tggaaaaatt gaccaatacc aagaattaga
     3121 tctaaaagca tctaaagaag cgtcgtacat tgaggctaaa aattctgctt cggcaggctt
     3181 gtcgcttttg tcagtaattt ttaaagtcca ttcctcattt gagccgggta aagttttatc
     3241 tctaaaactt gtgaatttaa tcgacaattt tttattttcc caaggcacac taatattttc
     3301 agaaaagcta taaagtctat tgttttttat aaacataaga tttattccca atcctcctaa
     3361 ataagactcg tcaataggaa tttctatttt tttaacttct gaattaatgt ttaatctttg
     3421 tacttgttga ttttctcctt ttttggttag tgtgtaaaat acttctacat ttttatatga
     3481 agaacccaaa taaatattta cggtttcgcc cacaaaagcg gtatttttat caacttgaaa
     3541 gaaatctgtt gcagtatatg gcatttgttt agagctacta gaataaacaa caatattttg
     3601 cacatcgctt atttcattat tccatttatc ttttgatttt aaaacaatac gataaactcc
     3661 tgcttcccat ttgtcagatc catttagttg tattttatta gtgttttcag tatttatatt
     3721 tgatttgaat acagactttt cagtttccca attttcaaat tttgtttctg cttcatattc
     3781 atatcccgga ttttttgaat accactcttc ttttgaatat aatttattgt caacttcatc
     3841 taacatgcgt tttgctaaca ataattttgg gtcttttagt ttaaaaattt ctatatcaac
     3901 ttttgctggc acagaaactc cttgaatatt ggtagtcttg atatttatac tgtctaaatt
     3961 tgctttttca ataattccag aaagattatg agaaatagat aaagctttgt ttgaaacata
     4021 tacatagccc gaagtttgct gagtttctcc gtttatgtca gtaacaaaaa ccttgattgt
     4081 ataattataa taaatatact catttattgt gctttcatct tcgggtatag ctttaaaatt
     4141 aatttcaaat tttccattat catcaagttt tgtttcacca aaagcaattt cctttgaaga
     4201 aaaagaatat ggcgaatatc ttctccaaat tggattacgc acaacagaat atgtaacttt
     4261 agcatctgtt aaggcagttc ctgcataagt tgtggcttta ccttgagctt taatactgtc
     4321 gtttactcta tattcgcctt caacaggaag catttttact tcaaaatttg gtcgtttata
     4381 ttcttctact ttaaagctaa tgcttccatc gttagtttga attgaaaaat ttccagcaag
     4441 aacatctaaa ggaattgtaa acgagccatt aaaacttcca tattcattag tttttacagt
     4501 agtttgatga atattttggt gatttacatc gcgtacaaat aaacttgttg aataatttgg
     4561 taaaatttta gcttcttcgc cttttttttc aatacaaatt cctttgtaat aaacagtttg
     4621 tccaggtcga taaattcctc tatcaataaa aaatttaatt tctatttgac ttttttcttc
     4681 atgttttcct cgtgaataaa cataaaaact ttcagataaa taatcatcat ttttacttac
     4741 atcaaaacgc aaatttgagt aagaattttt ttccgagata tttaaagtta tttctccatt
     4801 ttcatcagaa aattttgtac taaccaattt gtcaacataa tctcgcttaa tgtgcgaata
     4861 ttcatttttg tacatttcaa cttttgctcc ttttactggt gagcctgttt ttcgatttaa
     4921 aacataaatt ttagaagttg aataatcatc taattccgtt agtgctaagt cagatacaaa
     4981 aatagaatta gtagaaatat agtttttttc aaaatttaat tctggttcag catggagaat
     5041 tatatagtaa aaaccttttg acaagccatc aactaaataa tcagtgtaat gagaattgta
     5101 gtcgtcagtt tttggcaagt ctatttcttt cgaatttaca acagttttag aagattttat
     5161 tagtttttca tataattctt tattgtaata ttttctttct aatgtaagat atgtattgta
     5221 atcaagcgac aagattgtta aataaatttt atcagtattt ttataagaaa ttcttaatgg
     5281 aaaattatcc tcactactta caattttttc agatgtaaac gacaagttaa atttttctat
     5341 agtgtttttt aagttctcac attcagcact atacaaagcg tctggatata atttaagagc
     5401 ttcatcaagt aaggctattg ctgttttgtt ataatcttta tatttggcag ttgtgggatt
     5461 tttaaagtca tattgcgaag ccaaattaga ataataattg gctattttat aagctgtaag
     5521 tgtagattct ggatttttag aatatttttt atgtaaattt tctactgttg taagccatag
     5581 tttttctttt tctattgaat taaaatttct taatacaaaa tctaatctgt caatatcaac
     5641 cattaaaagt gatgccaggt tgtcgttttt atttaatttt acttctagcc aatcttgata
     5701 tatttttaat accgaataat taaaagataa agaatcataa tcaaacttaa tttttgtaaa
     5761 atcttcagca tcattaaaaa atgaattgtt tgtaaatttg tcttcatttt gagcagaatt
     5821 atcatagtaa taatatcttc tatactgacc cgtactcgtt aatcctttta ttgcagaatg
     5881 agcaacaaaa tcaagcaagg tttcgaattt tggcattgag ttttctgcat taattattat
     5941 ctctttatag tcttttattg gtatatcgtt taaagattta tctatagcta gcaaatagtt
     6001 ttttataatc ttatcttgaa atcttgtttt gtcccaagtt tgcatatcgt ttaaatcgaa
     6061 attataggta acagaacgtt gatttattaa ataagaatta ttggaataat attgagaata
     6121 catttttgct aataataaat gcaacatggg ttttactgca cccgaagatt ttgccgtttc
     6181 tatttcaagc tttgaaatag ctttttcata atcattacct tccgattcta aaatagcagt
     6241 atattgtgtt atataaataa gtgacttaat tatttgtaca tcttcattct cgcttaatgc
     6301 taaagcgtaa attttttcta ctttttctaa agcagatttt ggtaaagaat ttttttgaaa
     6361 ttcttctact tctttccata aagattcata tttgtctttt ttattaatat tgttctgtgc
     6421 tattgaaaaa atcaccgcaa ttagcgtaat agacagaaaa aaagcgaatt tttgaattaa
     6481 agatttcata tttttaatct aatttattta gttttaagat gaaaaattat taattttcca
     6541 taaaaatata atatttattt gaaaaaaata tataactttg gaaattataa tctttaatgc
     6601 attaaattat gaaaaatttt tacagtattt tatttttgtg tttggtttca acctttattt
     6661 ttggacaaac tcaaaccaca cgtgaattag tttgggattc tcaaacaaga cagtatattg
     6721 agtatattcc cactgcttac aattcagaaa atcctacgcc tgtgatgttt tgcttgcatg
     6781 gacttggaga tgatatgcag aattttcaca attcactaga actaaatcaa tggggcgaaa
     6841 cagccaactg gattattatt acacctcaag ctcttgatgc acaaattcct ataatgggtt
     6901 ctcttggcaa tgcttggaat tctggtgctg gtgctgaagg aataccaata ctaggtactg
     6961 ttattttaaa cgataatgtt gatgatgctg gtttcttaat ggcaatttta gactctttag
     7021 aaaataatct aaatattaat accgactctg tgttttttat gggattttct ttaggaggat
     7081 ttatgagtaa tagaatggct atcgaacatg gagatagaat tacagcaatt gcttctgtaa
     7141 gtggaacaat aggcatggca gtaaaagacc aaattccagt ggctaaagtt aatacattac
     7201 attttcatgg aacaaatgat gaaactattg cttatgaaga tgcaggattc cctataggtg
     7261 gaacaaatta ctctactggt ctgggagcag aacaaactgt tgactattgg aaaacacata
     7321 accaatgtgg aaacgaagca actcacactt ttttccctga tgaaaaagaa gatggtttaa
     7381 cttttgaaag atttttgtat aaaaacgaaa gtgaaaatat tcagactgct ttgataaaag
     7441 caaataatgg aatacatgat tggtattaca cgccagtaaa tgatattcat tattcaatag
     7501 aaatttataa gttttttaca aatacaatgg attttcctac agacattaaa aatacaacaa
     7561 ttgaaagttt aaatatttat ccaaatccag cttcagacat tctttttgta agtcaagatg
     7621 ttgatgagat tgcaatattt gatgttacag gaaaattagt tttaaaagaa tttaataata
     7681 atagtattaa tatttctgct ttaaatagtg gtttgtatat tgtaaaaact atttctgaag
     7741 gaaaaaatta cgagcacaaa atacagatta taaaataagt cagttaaaag tttataaagt
     7801 tcgtaaagtt cataaagttg gaccgagctt gggagctacc gaagataaag agtttttagt
     7861 gttgagtttt tagtttttag tagtgatttt ttaaaaactt cacactgata aaatttcttt
     7921 aatgcaaggg gtgtctatac ttgcttcgca gagttcgcat ggctcactct gcttgctagg
     7981 tttacacaat ttgttaaaat ctgttaacaa acagttgata agttgttaat aagttttatt
     8041 aaatttaatt gcattaatat ggttttgcat ctatatttga aacgataatt aagtagaaat
     8101 ttaaaataaa ggagtcatga aagcaaaact aattttattt gcaatatccc taacaattag
     8161 tggatttatt
//
LOCUS       DAOPNX010000105         8114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_9890_length_8114_cov_7.041196, whole genome shotgun sequence.
ACCESSION   DAOPNX010000105 DAOPNX010000000
VERSION     DAOPNX010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8114
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9890_length_8114_cov_7.041196"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="PKN53_09695"
     CDS             <1..858
                     /locus_tag="PKN53_09695"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOD89261.1"
                     /translation="KIAIVSPAGAINPDFISSASNLMKNYGFQPIIFPSCIKKHFQFA
                     AKDEERLEDLQAALDDKDIKAILCSRGGYGLIRILDKLNFDKFLKNPKWIIGFSDITN
                     LHIALNNHFVMSIHGLMAKAMHEEPEAEAVKNIFKILNGEKPKIICENHEFNKNGFAQ
                     AEIIGGNLSIIYSLQGTKFEINTDGKILFIEDLNEYLYHLDRMMLSLKMAKKFDNLKA
                     LIVGNFTDMKDNPNPFGKTAYEIIAEHVAEFDFPVCFNFPIGHIDNNMPIICGQEYKL
                     EISEKVILS"
     gene            882..1826
                     /locus_tag="PKN53_09700"
     CDS             882..1826
                     /locus_tag="PKN53_09700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD89262.1"
                     /translation="MDYKSNIHCEVCGNKDVDKFSLKYTRADFQVTDCDVCGFSFIPQ
                     FYRKQIPYENYRDDEVLKSVRAGNNYIKFRRHKLRIKLIKKFQKTGKLYDVGVGWGHF
                     LHTAQVLGFEASGVEISELMHHYATTDLKLNVVHDDFYNLELPENNWDVVTMWDVLEH
                     ISEPQKAVKKINKMLKKDGFFVLQVPQIDSKVAKKQKQNWSMMSIEHINYFSKKTIKT
                     LLEDNGFEIVKIKSSYELKLFLMFTVLPWLKRKKTKKQDEKITITNAERQNFFNKFTK
                     LPKFVIWLGLLAHDIVYKFMSAINYGEEMIVVAQKKTE"
     gene            1964..4474
                     /locus_tag="PKN53_09705"
     CDS             1964..4474
                     /locus_tag="PKN53_09705"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOD89263.1"
                     /translation="MKRNILILFFILSFCSAHAQEKDSIPTGQQMDIPTITLTENELE
                     SDMQNQNISGLLQSSQDVFMNTAGYTFGQARFRIRGYGNENFLVLLNGLTLNDAETGR
                     PYYYYWGGLNDATRNKVVTSGLHFYDYSYGALNGVTNILTRASEYRPGTSISYSLANK
                     SYRNRVMVTHATGLMSNNWAFTFSASSRWANEGYVKGTFYNAYAYFFSAEKKINDKHS
                     INLTAFGSPSRTGRAGVATLEAYELSGDNFYNPNWGYQNGVMRNSRVGNTHQPRVLLS
                     HYWKIKPDMQLTTSASYLFGRNGVTALNWYDAADPRPDYYRNLPSYYEEGEYMFNYLT
                     DAWLNQENYGQLDWDYFYFANRKNLYTIVDESGIEGNNVTGNRAIFMVEERRNDIKRF
                     DFNSNLSKQLGENGKLVAGVNLMMNKTHNFKTVSDLLGADWWVDIDQFAERDNADDNY
                     YVQTNLETPNKAVKEGDIFGYYYISNINKADAYGQVEYKFNKIDFYVALNLSYTDFWR
                     TGKMKNGKFPLNSYGDSPNKTFFNYATKAGMTYKITGRNFILANATYMTRAPYFRNAY
                     VSPRTRDNVVSNLSSENIFSGDLSYLFRSPYLVAKITGYYTEFRNSIESRNFYHDELR
                     TFVNYIMTDLNKVHYGGELGVEAKVSPTVSLNTALGYGKYLYNNRPNITIVADNSAEV
                     LVEEKVAYIKNYHLGGSPEMAASLGGKYSAPKYWFISINANYFANSYVTINPERRTEE
                     IFSMFVESDPQIESILEQEKLKPVYTFDIWGGKSWRIKRKYTIGFNLSINNILNNTNF
                     LTNGFEQLRFDRTKIDKFPNKYYYMYGRSYFLNVYFRF"
     gene            4493..5284
                     /locus_tag="PKN53_09710"
     CDS             4493..5284
                     /locus_tag="PKN53_09710"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HOD89264.1"
                     /translation="MKNKLSILISLSLIIAAFVFYSCEKELDRPPIKVLDESQILTIK
                     DLYDIHAEKGDDYVFTEDYMLFATVAMDDSSGNIYKEAYLQDSTGGINLYKLSHAGVL
                     KVGQYVRINLNGSSIKNYNGKMEIIFAKVLDFRKSMVVQSYGQFIEPVEIDNLNQIYE
                     GTWDCQLVKIKNVEFIPGDTSKTYANLGGSSAQNRTVQDCNGKTLVIRTSDYAKFAGT
                     ELPKGNGDIIGIITKYVNSYTNTVTWQLLVRDPKEVNLNNPRCGS"
     gene            5296..6570
                     /locus_tag="PKN53_09715"
     CDS             5296..6570
                     /locus_tag="PKN53_09715"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HOD89265.1"
                     /translation="MKNFKILSKIALAIFIALFSLSLESCIHDNFEEPPTIDITEGTT
                     LTLDQIYQIYNDSVLAKGKQYYKFVDDYSVCAVVTMDDKSGNIYKSAYVQDVSRGINL
                     HLMSSGGLYVGDSIRIKLKGLVLSEYAGMMQIDSVKVVDNIVKLATLKNIQPETVTID
                     QILTGQFLAKLVKIENVQFIESDLNTKFADGENLITLNKTLEDEQGRTIVVRTSGYAS
                     FAKMDIPSGRGDLIAIVGKYNDTWQLLLRSIYEVNFTKRRFGDFDIIFSEDFAGITNG
                     TEVNLAGWYNIAKVGSLKWMGFNNTQGAEYVKIDGNSTAAETYLILPEQQLNNNKMSF
                     RTRAGNLIGAKLELVISNDFDGSNIETATWTKIPASFATAPTSGFGSWLESGEIDLSS
                     YSGNYYLAFRYVAETGQKATFMIDDIIIYTNE"
     gene            6601..7851
                     /locus_tag="PKN53_09720"
     CDS             6601..7851
                     /locus_tag="PKN53_09720"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HOD89266.1"
                     /translation="MKKIIICLFFFNLYFSSFSQNHEQDILNLEFRLIELNAEIDSVL
                     NLLEDLKLKALKADLLENGLPEILEDEELICHSAFCLVYDDNHKMAKWTAHIISREIV
                     NGVVSRTNDFRVDSLIKNGTAEEEDYFLTKKDESGNTIYDGFGYDRGHLAPSADFRWS
                     EKALSESYYYSNMTPQLPEFNREIWAKIEDFLRQYIYNNPTKDIYIVTAPVIKDDLKK
                     QERSKNKISIPEYHYKIAVDLEDKKGIAFLVPQKNLGNPIEYYVVTIDSIENITNINF
                     YPKLSEQDEKLIESESDIGKWRSGRSKNDQTPLDIKTLPKNSYNTVNARQFNEYPKEV
                     NICGTVVSTHKSRNGHIFLNLDKSFPNQVFTATIWKSNTPNFSYEPEKFLLNKKVCIK
                     GKVKDYKGTPSTYPENEKAIKILE"
BASE COUNT         3030 a         1139 c         1327 g         2618 t
ORIGIN      
        1 aaaattgcaa ttgtcagtcc tgctggtgct ataaaccccg attttatttc atctgcttct
       61 aatttgatga aaaattatgg ctttcaacca ataatttttc cttcttgcat aaaaaaacat
      121 tttcagtttg ctgcaaaaga tgaagaaaga ttagaagatt tacaagcagc tttagacgac
      181 aaagatatta aagcaatttt gtgttcgcgt ggcggttatg gacttattag aattttagat
      241 aaattgaatt ttgataagtt tttaaaaaat ccaaaatgga taataggatt tagcgatata
      301 acaaatttgc acatagccct aaataatcat tttgtaatga gtattcatgg tttaatggct
      361 aaagctatgc acgaagagcc agaagcagaa gctgtaaaaa atatttttaa aattctcaat
      421 ggtgaaaaac caaaaattat ttgcgaaaat cacgaattta ataaaaatgg atttgcacaa
      481 gccgaaatta ttggtggaaa tttatccata atttatagcc tgcaaggaac aaaatttgaa
      541 attaatacag atggtaaaat tttgtttata gaagatttaa acgaatactt atatcactta
      601 gacagaatga tgttaagttt aaaaatggct aaaaagtttg ataatttaaa agctcttatt
      661 gttggaaatt ttaccgacat gaaagataat ccaaaccctt ttggaaaaac agcttacgaa
      721 attattgcag aacacgtagc tgaatttgat ttccctgttt gctttaattt tccaataggt
      781 catatcgaca acaatatgcc aataatttgc gggcaagaat ataaattaga aatttcagag
      841 aaagttattt tgtcttgatt tttctttatt tttgcaaaat tatggactat aaatcaaata
      901 tacattgcga agtttgtgga aataaagatg ttgacaaatt ttcactaaaa tatactagag
      961 ccgattttca agtaacagac tgcgatgttt gcggattttc ctttattcca caattttata
     1021 gaaaacaaat tccttacgaa aattaccgcg atgatgaggt tttaaaatct gttcgtgcag
     1081 gaaataatta tattaaattc cgtaggcata aactcagaat taaactaata aaaaaatttc
     1141 aaaaaacagg aaaactttac gatgttggcg ttggttgggg acatttttta catacggctc
     1201 aagttttagg tttcgaagca tcaggagtag aaatttctga actaatgcat cattatgcta
     1261 caacagattt aaaattaaat gttgtacacg acgattttta caatttagaa ttacctgaaa
     1321 acaactggga cgtagtaaca atgtgggacg tgctagaaca tatttcagaa ccacaaaaag
     1381 cggtgaaaaa aattaataaa atgcttaaaa aagacggttt ttttgtttta caagttccgc
     1441 aaatagattc taaagttgcg aaaaaacaaa agcaaaactg gtcaatgatg tcaattgaac
     1501 atattaatta tttttctaaa aaaactataa aaactctttt agaagacaat ggatttgaaa
     1561 ttgtaaaaat taaatcttca tacgaattaa aattattttt aatgtttaca gttttgcctt
     1621 ggctaaaacg taaaaaaaca aaaaaacaag acgaaaaaat tacaatcaca aatgctgaaa
     1681 gacaaaattt ctttaataaa tttaccaaac taccaaaatt tgttatttgg cttggattac
     1741 ttgcccatga tatagtttac aagtttatgt ctgctataaa ttatggcgaa gaaatgattg
     1801 ttgttgctca aaaaaagaca gaataaaaaa tgctgtaaac tataagtttt caactttatc
     1861 aacttcgata ttaacaaatt gttaaaaaaa acaagtaaaa aaattatttt gtgtgttttt
     1921 taagactaaa tttgtcgaaa taaattactt aacatttaat aagatgaaga gaaatattct
     1981 tattttgttt tttatacttt ctttttgtag tgctcatgcc caagaaaaag atagtattcc
     2041 aacgggacaa caaatggata tcccgacaat cacacttacc gaaaacgaat tagaaagtga
     2101 tatgcaaaat caaaacattt caggattact acaatcctca caagatgttt ttatgaatac
     2161 tgcaggttat acttttggac aagcacgttt tagaattaga ggttacggca acgaaaactt
     2221 tttggtttta ttaaacggtt taacattaaa cgatgccgaa actggtcgtc cttattatta
     2281 ttattggggc ggtttaaatg atgctactag aaacaaagtt gttacttcag gtttgcattt
     2341 ttatgattat agttatggag ctttaaatgg agttacaaat attttaacta gagcttctga
     2401 atatcgtcct ggtacaagca ttagttattc gttagctaat aaaagttata gaaacagagt
     2461 aatggtaact catgcaacag gtttaatgag taataattgg gcttttacgt tttcagcctc
     2521 atcacgttgg gctaacgaag gatatgtgaa aggaactttt tataatgctt atgcatattt
     2581 tttctctgca gaaaaaaaga taaacgacaa acatagcatt aacttaactg cttttggttc
     2641 gccttcacgt acaggaagag ctggcgttgc aactcttgaa gcttacgaat tgagtggtga
     2701 taatttttat aatccaaatt ggggttatca aaatggagtt atgagaaatt caagagtagg
     2761 aaatactcat caaccaagag ttttattatc acattattgg aaaattaaac cagatatgca
     2821 attaacaact tcggcttcgt atttgtttgg acgaaacgga gttactgctc taaactggta
     2881 tgatgctgct gacccacgtc cagattatta cagaaatctc ccaagctatt acgaagaagg
     2941 agaatatatg tttaactacc ttactgacgc ttggttaaat caagaaaatt atggacagtt
     3001 agactgggat tatttttatt tcgcaaatag aaaaaactta tacactattg ttgacgaatc
     3061 tggaatcgaa ggaaataatg ttacaggcaa tagagctata tttatggtag aagaaagaag
     3121 aaacgatata aaaagatttg acttcaactc taatttaagc aaacaactcg gcgaaaatgg
     3181 aaaattagtt gcaggagtaa atttgatgat gaacaaaacc cataatttta aaacagtcag
     3241 cgacttgctt ggagctgatt ggtgggttga tatagaccaa tttgccgaaa gagacaatgc
     3301 tgatgataat tattatgttc aaaccaactt agaaacacct aataaagctg ttaaagaagg
     3361 cgatattttt ggatattatt acatttcaaa tattaacaaa gctgatgctt atggacaagt
     3421 tgaatataaa ttcaataaaa tagatttcta cgttgcatta aatctttcat ataccgattt
     3481 ttggagaaca ggaaaaatga aaaatggaaa attccctctt aattcttatg gcgattctcc
     3541 taacaaaaca tttttcaact acgctaccaa agctggtatg acttataaaa ttactggacg
     3601 caactttatt cttgccaatg caacatatat gacacgtgct ccatatttta gaaatgctta
     3661 tgtttctcca agaactcgtg ataatgtagt ttcaaatctt tctagcgaaa atattttttc
     3721 aggagattta agttatttat tccgttctcc ttatcttgtt gcaaaaatta ctggttatta
     3781 cacagagttt cgcaattcta tagaaagcag aaatttctac cacgacgaat tgcgtacttt
     3841 tgttaactat attatgacag accttaataa agtacattat ggtggagaat taggtgttga
     3901 agcaaaagtt tcgccaacag ttagtttaaa tactgctctt ggttatggaa aatatttgta
     3961 caacaataga ccaaacatta caattgttgc cgacaactct gccgaagttc ttgttgaaga
     4021 aaaagttgct tatatcaaaa attatcatct tggaggttct ccagaaatgg cagcttcatt
     4081 aggtggaaaa tatagtgctc ctaaatattg gtttataagt attaatgcaa actattttgc
     4141 aaattcttat gttaccataa accctgaaag acgcacagaa gaaattttta gcatgtttgt
     4201 tgaatcagac ccacaaattg aatcaatttt agaacaagaa aaacttaaac ctgtatatac
     4261 ttttgatatt tggggtggta aatcttggag aattaaaaga aaatatacca taggatttaa
     4321 tttgagcata aataatattt taaacaacac aaatttctta accaatggct ttgaacaact
     4381 aagatttgac agaacaaaaa ttgacaagtt cccaaataaa tattattata tgtatggaag
     4441 atcgtatttc ctaaatgttt attttagatt ttaattaaaa aaatatagca aaatgaaaaa
     4501 taaattatca atactaatat cgcttagctt aataattgcg gcttttgtgt tttattcttg
     4561 tgaaaaagaa ctagatagac caccaataaa agtattagat gagtctcaaa ttttaaccat
     4621 taaggatttg tatgacattc atgctgaaaa aggagacgac tatgttttta ctgaggatta
     4681 tatgctattt gctactgttg caatggacga ttcgagtgga aacatataca aagaagcata
     4741 tttacaagat tctactggtg gaatcaatct atataaactt agtcatgctg gagttttgaa
     4801 agttgggcaa tatgtaagaa ttaatcttaa tggaagtagt ataaaaaact ataatggtaa
     4861 aatggaaata atttttgcca aggttcttga ttttcgcaaa tctatggtag tacaaagtta
     4921 cggacaattt attgaacctg ttgaaattga caatcttaac caaatttatg aaggcacttg
     4981 ggattgccaa ctagtaaaaa taaaaaatgt agagtttatt cctggtgata caagcaaaac
     5041 ttatgcaaat ttaggaggta gttccgcaca aaatcgcaca gttcaagatt gtaatggaaa
     5101 aacattagtg attagaacta gtgattatgc aaagtttgca ggaacagaac taccaaaagg
     5161 aaatggtgat ataattggaa taattaccaa atatgtaaac agttatacca atactgtaac
     5221 atggcagtta ttagtccgcg acccaaaaga agttaattta aacaatccac gttgtggttc
     5281 ttaaaaattt agattatgaa aaattttaaa atattatcaa aaatcgcttt agcaatattt
     5341 attgctttgt tttctttgag tttagaatct tgtattcacg acaacttcga agaaccacct
     5401 actattgata ttactgaagg cactacgcta actttagacc aaatttatca aatttataat
     5461 gatagtgttt tagctaaagg taaacagtac tacaaatttg ttgacgacta ctcggtttgt
     5521 gctgttgtaa caatggacga taaaagtgga aatatttaca aatctgctta tgttcaagat
     5581 gttagcagag gaataaacct gcatttaatg tcttcaggcg gactttatgt tggagattca
     5641 attagaatta aattaaaagg tttggtttta agcgaatatg ctggaatgat gcaaatagac
     5701 tctgtaaaag ttgttgataa tattgtgaaa ttagcaactc taaaaaacat acaacctgaa
     5761 accgtaacaa tagaccaaat tcttactggt caatttttag caaaacttgt taaaatagaa
     5821 aacgtacagt tcattgagtc tgatttgaat accaaatttg ctgatgggga aaatttaatt
     5881 acactaaata aaacattaga agacgaacaa ggaagaacta ttgttgttag aactagtggc
     5941 tatgcaagtt ttgcaaaaat ggacattcct tctggcagag gcgacttaat tgctattgta
     6001 ggcaaatata atgacacttg gcaattattg cttcgttcga tttatgaagt taattttact
     6061 aaaagacgtt ttggcgattt tgacattata tttagtgaag actttgcggg tattacaaat
     6121 ggtacagaag taaatttagc tggctggtat aatattgcta aagttggttc tttaaaatgg
     6181 atgggattta ataatacaca aggtgctgaa tatgtaaaaa ttgatggaaa tagcacagca
     6241 gcagaaactt atttaattct accagaacaa caacttaata ataataaaat gtctttccgc
     6301 acgcgtgcag gtaatttaat aggagcaaaa ttagaattag taatttccaa tgattttgac
     6361 ggttcaaata ttgaaactgc aacttggaca aaaattcctg caagttttgc aacagcacca
     6421 acaagtggtt ttggaagctg gcttgagtct ggcgaaatcg accttagttc atattctggc
     6481 aattattatt tagcatttag atatgtagca gaaactggtc aaaaagctac ttttatgatt
     6541 gatgatatta ttatttacac caacgaatag agctttaagt tttaaataaa atatgtctta
     6601 atgaaaaaaa ttattatttg cttatttttt ttcaatttat atttttcaag tttctcacaa
     6661 aatcatgaac aagacatatt aaatttagaa ttccgtttaa ttgagcttaa tgctgaaata
     6721 gacagtgttc ttaatctttt agaagactta aaattaaaag ctctaaaagc tgatttgtta
     6781 gaaaatggtt tgccagaaat attagaagat gaagaattaa tatgccattc ggctttttgt
     6841 ttagtatatg acgataatca caaaatggct aaatggacag cacatattat ttcacgcgaa
     6901 attgtaaatg gagttgtttc tagaaccaat gactttagag tggacagctt aataaaaaat
     6961 ggaactgccg aagaagaaga ttattttcta acaaaaaaag atgaaagtgg aaatactatc
     7021 tatgacggtt ttggatacga cagaggacat ttagctcctt ctgccgattt tagatggtca
     7081 gaaaaagctc tttcagaatc atactattat tccaatatga caccacaact tccagaattt
     7141 aatcgtgaaa tttgggcaaa aatcgaagat tttttaagac aatacatcta caacaaccca
     7201 acaaaagata tttacattgt tacagctcca gtaattaaag atgatttaaa aaaacaagaa
     7261 agaagcaaaa acaaaatttc tattcctgaa tatcactaca aaattgcagt tgacttagaa
     7321 gataaaaaag gaattgcttt tttagtacca caaaaaaact taggcaatcc tattgagtat
     7381 tatgttgtta caattgattc tatagaaaat atcacaaata ttaattttta tccaaaactt
     7441 tctgaacaag atgaaaaatt aatagaatct gagtcagata ttggcaaatg gcgttcgggc
     7501 agaagtaaaa acgaccaaac tcctttagat attaaaaccc tacctaaaaa ttcttacaac
     7561 actgtaaacg caaggcaatt taatgaatat ccaaaagaag ttaatatttg tggaactgtt
     7621 gttagcacac acaaatctag aaatggacat attttcttaa acttagataa aagttttcca
     7681 aaccaagtat ttactgcaac aatctggaaa tcaaacactc ctaacttttc ttacgaacca
     7741 gaaaaatttt tacttaacaa aaaagtttgc ataaaaggaa aagttaaaga ttataaagga
     7801 actccatcaa cttatccaga aaatgaaaaa gcaataaaaa ttttggagta atttatttta
     7861 actagaattt tttattattt aatcacacaa tatttcctgt cttccgcact ttttctacag
     7921 tacccgttaa gtctatgata atcaggcaat ttattgtgtt taggtgctgc aacattttaa
     7981 acagcatata catatataat tgtccgtaag gacaatataa gactatcaag ggtttgtccg
     8041 taggacaaca tgtgattaag aggctgtctc aaaagtaaaa acagcctctt ttttttcacc
     8101 ttttttcttt gcta
//
LOCUS       DAOPNX010000106         7877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_10300_length_7877_cov_6.342751, whole genome shotgun sequence.
ACCESSION   DAOPNX010000106 DAOPNX010000000
VERSION     DAOPNX010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7877
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10300_length_7877_cov_6.342751"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3170
                     /locus_tag="PKN53_09725"
     CDS             <1..3170
                     /locus_tag="PKN53_09725"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOD89267.1"
                     /translation="MTEKLFKRLDYAVGWFTFLVAATVYLLTIEPTVSFWDCGEFISS
                     SNKLQVGHPPGAPFFMLIARTFAMLASGPDKVALMVNSFTAIVSAFTIMFLFWSIVYF
                     AKRMIAPNEDYTVGKTIAILGAGLVGALVYTFSDTFWFSAVEAEVYGFSSFFTAIVFW
                     AILKWSDEKDPIKENRWIVLIALLVGMSIGVHLLNLLTLPAIAFVYYYKKFKPSTLGF
                     IITTIISLFIIAMLMWGIIPGVAIVASKFELFFVNSLGFGYNTGLIIWAILAFASLGI
                     SIYTSQYSKNKTLNYIFPSLAIILIGAPFISDSTILNILILGACIAGIVYLASRKREI
                     LNLIMLSFTMVMIGYSSYAIIVIRSNANPPMDQNSPDDVFNLLYYLNREQYGDRPLMY
                     GEYYNARFEKYEKTTPVYVKRNGEYQIVSYKQKPTYKKSDCTVFPRMYSPEPKHIEYY
                     KEFGGIKNTQRRPTFLNNIKFFVNYQINWMYWRYFMWNFAGRQNDIQGHGNVIHGNWI
                     SGIKFIDNNRLGDQDKLPDYLKENKANNKYYMLPLLLGLIGLGYQLFKHIKDWWIVTL
                     LFILTGIAIVVYLNQTPEQPRERDYAYAGSFYAFAIWVGLGVLGIYELLKKVSPGVVA
                     GSVATLITLPVPYIMAKENWDDHDRSNRYIARDFAYNYLISCAPNSILFTFGDNDTFP
                     IWYSQEVEGVRPDVKVCCLPYFASDWYIDQMKAASYDAAPMPLTFERPKYEPSVRDVV
                     QYAYRDIRKDEVPISIDSLLNFIANDNVATYKYNDDTYYIYPSNKYYMIVDSAKVVDN
                     KTVKPEDAGLISTRIDADLNRRYLVKNEMMTLDFLRTNDWDRPIYFTSIGSSNAVGLD
                     NYFQNEGFAFRLVPIKSSPRGRIDKDIMYDNLMNKYRWGNMNDPNIYIDHTINRTTTV
                     VRIRENFSSLVLEFAKVGDTVKAKNLLAKCEEIMPTSIFRPNLYDVDFANAYYSIGEN
                     ERGDKFLKEIIEISSQELNFFFGLDNNKRNSCLFEIQVSMETLNRVWRVAQRNKRDEL
                     LSELEVSLTDFMQKYQTYYPEYFRQ"
     gene            3418..4761
                     /gene="purB"
                     /locus_tag="PKN53_09730"
     CDS             3418..4761
                     /gene="purB"
                     /locus_tag="PKN53_09730"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006045444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOD89268.1"
                     /translation="MNKESLNALSPVDGRYKAKTEILRDYFSEFALIKYRIEVEVEYF
                     IALCEIPLDELKDFFRPKFEKLREIYNNFSIEDAQRVKEIEKTTNHDVKAVEYFLKEK
                     FDSLQLNQYKEFIHFGLTSQDINNTAVPLSVKDCMNEVYSPSLLNIIKLLDDLTDEWK
                     DVTILARTHGQPASPTRLGKEIQVFSTRLKEQLTQMEQIPFSSKFGGATGNFNAHLVS
                     YPNIDWNEFGFHFVNNYLGLHRSFPTTQIEHYDNLAALLQCIGRINTILIDFCRDMWT
                     YISMDYFKQKIKAGEVGSSAMPHKVNPIDFENAEGNLGIANAIAHHLAEKLPISRLQR
                     DLTDSTVLRNIGMPFGHTIIAFESIKKGIGKLLLNQEQINKDLENNWIVIAEAIQTVL
                     RKENYPNPYEALKELTRTNTKITQQTFIDFINNLDIKEEIKEQLRKITPYNYTGI"
     gene            4768..6591
                     /locus_tag="PKN53_09735"
     CDS             4768..6591
                     /locus_tag="PKN53_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD89269.1"
                     /translation="MKDFYKIIKYIFPYKVQAILNIFFNVLSTIFSVFSFAIVIPFLG
                     ILFKTQALVTEKPALDITSVSSLQHNFNYWLSNIIIEHGEIKALISMGVLVIIATFFK
                     TLCFYLANHFMVPLRNGVVKDIRNQLFDKVISLPIGYFSEEKKGNIIARMTNDVQEIE
                     WGIMSSLEMIFRDPIKILIYLGTMFIISWQMTSFVFILLPIVGLIIGRTGRSLRKVSM
                     TGQQQMGSLMAQIEEMIGGLRIIKAFNAEKHISDKFKDTNKDYTGLMNFVNRKRFLAS
                     PLSEFLATIAVVVIMWYGGTLILGDNALLSPAALIGYLVIFSQIISPAKTLSSSWFNI
                     KKAMASVDRVNDILDQTNPIVEKQGATEITEFKNSIEFKNVNFAYTEDIPVLKNINLK
                     IQKGQTVAIVGQSGSGKSTMVDLIPRFYDILEGEILIDGINIKDYKLSNLRELIGYVH
                     QTPILFNDSIANNIAFGKQNVSQKNIEEAAKVANAHDFILESENQYDTNIGDGGCKLS
                     GGQRQRLSIARAVLKNPPILILDEATSALDTESENLVQEALDNLMQNRTSIVIAHRLS
                     TIKRADLICVLHEGQIVETGKHEELIAKKGMYYKLNNIQNG"
     gene            6636..7163
                     /locus_tag="PKN53_09740"
     CDS             6636..7163
                     /locus_tag="PKN53_09740"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOD89270.1"
                     /translation="MKRKLIYFSIICTLLVACGSNDKPKRPDGMAKNTGELKFQDYNF
                     ENPPQFRKDGDLRFYDSNTQEELFHIETEVASTDESRARGLMFRKEMLENQGMFFLFP
                     KDEIQAFYMRNTLIPLDILYVNSKMEIVDIYKNTKPKDETSLPSKEPAKYVIEINAGL
                     CDKYNIEIGDYIVFD"
     gene            complement(7160..7744)
                     /locus_tag="PKN53_09745"
     CDS             complement(7160..7744)
                     /locus_tag="PKN53_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89271.1"
                     /translation="MKFKTTILLFAIVLALFSCSQSTKKNEAEEEIKPCDYENDIELT
                     IADYYYSMSDTLVVENYKFEVKQSEMEWINDTTISLKLKNYSEEEFNGRLAENQYDIL
                     IGINARQGKKISDVYYAYLDYQSGLWCQVNIVTCFGEVYFNWLSGMPAQGGITIDYVD
                     KDNICGVFNLNVENLDSPLIGIVKLNGKFTHTKD"
BASE COUNT         2883 a         1172 c         1282 g         2540 t
ORIGIN      
        1 ctatgactga aaaattattt aaacgactag attatgcagt cggctggttc actttcctag
       61 ttgctgcaac agtatattta ttaactattg agcctacagt tagcttttgg gattgtggtg
      121 aatttatttc ttcatcaaat aaactacaag taggacaccc tcctggagcg ccatttttta
      181 tgttaattgc gagaactttt gcaatgttag ccagcggacc cgataaagtt gcacttatgg
      241 tcaactcgtt tactgcaata gtttcagcat ttacaataat gttcttattt tggtctatag
      301 tttattttgc aaaaagaatg attgctccta acgaagatta tacagttgga aaaactattg
      361 ctattcttgg agctggttta gtaggtgctt tagtatatac tttttcagat actttctggt
      421 tttcggcagt tgaagctgaa gtttacggct tctcatcttt ctttactgct atagtatttt
      481 gggcaatttt aaaatggagc gacgaaaaag accccataaa agaaaataga tggatagtat
      541 taattgcact tttagtggga atgtcgattg gcgttcactt attaaacctt ctaactcttc
      601 ctgcaattgc ttttgtttat tactataaaa aattcaaacc atctactctt ggatttataa
      661 taaccacaat aatttcactt tttataattg caatgttaat gtggggaata atccctggag
      721 ttgcaattgt agcttcaaaa tttgaattgt tttttgtaaa tagtttagga tttggatata
      781 atacaggctt aattatttgg gctattttag cttttgcaag tttgggaata agtatttaca
      841 cgtcacaata ctcaaagaat aaaactttaa attatatttt tcctagttta gctattattt
      901 taataggagc accatttata tccgacagca ctatacttaa catcttaata cttggagcct
      961 gcatagcagg aattgtatat ttagcatcaa gaaaacgtga aattctcaac ttaatcatgt
     1021 tgtcttttac tatggttatg attggttatt ctagctatgc tattattgta attcgctcaa
     1081 atgcaaatcc gccaatggat caaaacagtc ctgatgatgt ttttaacttg ctatattatc
     1141 taaatcgcga acaatatggt gaccgtccac taatgtatgg tgaatattac aatgctagat
     1201 tcgaaaaata tgaaaaaact acaccagttt atgtaaaacg taatggtgaa tatcaaattg
     1261 ttagttacaa acaaaaacct acttacaaaa aaagcgattg tactgttttc ccaagaatgt
     1321 atagtcctga accaaaacat attgaatatt acaaagaatt tggaggaata aaaaacactc
     1381 aacgaagacc aacattctta aataacataa aattctttgt aaattatcaa ataaattgga
     1441 tgtattggcg atattttatg tggaattttg caggaagaca aaacgacatt caaggacatg
     1501 gaaacgtaat tcatgggaac tggatttctg gaattaagtt tatagataat aataggctcg
     1561 gcgatcaaga taaattgccc gactatctaa aagaaaacaa agcaaataat aaatattata
     1621 tgcttccact acttttagga ttaattggtt taggctatca actctttaaa cacataaaag
     1681 actggtggat agttacttta ttgtttattt taacaggaat agctattgta gtttacctaa
     1741 accaaactcc agaacagccg cgtgaacgag attatgctta tgctggttcg ttttatgctt
     1801 ttgccatttg ggttggactt ggagttttag gaatttatga actattaaaa aaagttagtc
     1861 ctggagttgt tgcaggctct gtagccactt taattactct acctgttcct tatataatgg
     1921 ctaaagaaaa ttgggacgac cacgacagaa gcaacagata tattgcaaga gattttgcat
     1981 ataattattt gatttcttgc gcacctaact ctatactatt cacttttgga gacaatgaca
     2041 ctttcccaat ttggtattct caagaagtag aaggagttag accagatgtt aaagtctgtt
     2101 gtttgccata tttcgcatca gattggtata tagatcaaat gaaagcagct agttacgatg
     2161 cagcacctat gccactaact tttgaaagac caaaatatga accttctgtt agagatgtag
     2221 ttcaatatgc atatagagac attcgtaaag atgaagtacc tatatctatt gatagcttac
     2281 taaacttcat cgctaatgat aatgttgcca catacaaata taatgatgat acatattata
     2341 tttaccctag caataaatat tatatgatag ttgacagtgc gaaagttgtt gataataaaa
     2401 ctgtaaaacc cgaagatgct ggtttaataa gcacaagaat agacgctgat ttgaacagaa
     2461 gatatcttgt caaaaacgaa atgatgacat tagacttttt acgtactaac gattgggata
     2521 gacctatata tttcacatct ataggtagct ctaatgctgt tggacttgat aattatttcc
     2581 aaaatgaagg ttttgcattt agattagtgc ctatcaaatc aagtccaaga ggaagaattg
     2641 ataaagacat aatgtatgac aacttaatga ataaatatag atggggaaat atgaatgacc
     2701 caaatatcta tattgaccat acaataaaca ggactactac tgttgttaga attagagaga
     2761 acttttcaag cttagtatta gaatttgcaa aagttggcga tactgttaaa gcaaaaaatt
     2821 tactagctaa atgcgaagaa ataatgccaa caagtatttt tagacctaat ttgtacgacg
     2881 tagatttcgc aaatgcttat tattctattg gtgaaaacga aagaggagat aagtttttaa
     2941 aagaaattat tgaaatttct tcgcaagagt taaacttttt ctttggatta gacaataaca
     3001 aaagaaattc gtgcttattt gaaattcaag tctcaatgga aactcttaac agagtatggc
     3061 gagtggcaca acgtaataaa agagatgagt tattaagtga acttgaagtt agtttaacag
     3121 actttatgca aaagtaccaa acttattacc cagagtattt tagacaatag tttcgaaatg
     3181 aggctgtctc aaaacatatt ttaaaacaat gaattttcaa ggtgtttcag aagtgttaaa
     3241 atgcaaggcg tctgaaattg aggacgaagg agcgtaccag aagcgatgca ctgagcaaag
     3301 ccgaagtgta cgtgactgag tccgaatatt gaaagcaacg tggttgctga acgaagttga
     3361 agtaaagcag tttgacactt atgagacacc gcctaatttt ataaaattaa tattaaaatg
     3421 aataaagaaa gtttaaacgc tcttagtcca gttgacggaa gatataaagc taaaactgaa
     3481 attcttagag attatttttc tgagtttgct ttaataaaat atagaataga agttgaagtt
     3541 gaatatttta ttgctctttg cgaaattcca ttagatgaac taaaagattt ttttagacca
     3601 aagtttgaaa aactaagaga aatttacaac aatttttcca ttgaagatgc acaaagagtt
     3661 aaagagattg aaaaaactac taaccacgat gtcaaagctg tagagtattt tttaaaagaa
     3721 aaatttgata gtttacagct aaatcaatac aaagaattta ttcattttgg cttaacttca
     3781 caagatataa acaatactgc tgttccttta agtgttaaag attgcatgaa cgaagtttat
     3841 tcgccttctc tattaaatat tataaaatta ttagacgatt taactgatga atggaaagat
     3901 gttacaatct tggcaagaac acatggacag ccagcctctc ctactcgatt aggaaaagaa
     3961 atccaagttt tttcaactag attaaaagag caactaaccc aaatggaaca aattcctttt
     4021 agttcaaaat ttggtggtgc tacaggaaat ttcaacgcac atcttgtgag ctatccaaat
     4081 attgattgga acgagtttgg attccatttt gtaaataatt acttaggttt gcatagaagt
     4141 tttccaacaa cacaaatcga gcattacgac aacctagcag ctttacttca atgtattgga
     4201 agaataaata caatattaat tgacttctgt cgcgatatgt ggacatatat ttctatggat
     4261 tactttaagc aaaaaattaa agctggtgaa gttggttcta gtgcaatgcc acataaagta
     4321 aatcctatag attttgaaaa tgcagaagga aatcttggaa tagcaaatgc aatagctcat
     4381 catttagctg aaaaacttcc tatcagccgc ttgcaacgtg acctaaccga ctcaacagtt
     4441 ttgcgaaata ttggaatgcc tttcggacat actataatag cttttgaatc aataaaaaaa
     4501 ggaattggaa aacttctgct aaatcaagaa caaattaata aagatttaga aaataattgg
     4561 atagtaattg ctgaagctat acaaactgtg cttagaaaag aaaattatcc aaatccttac
     4621 gaagccctca aagaacttac cagaacaaac actaaaatta ctcagcaaac tttcattgat
     4681 tttataaaca acttagatat aaaagaagaa attaaagaac aattgagaaa aataacacct
     4741 tataattata caggtatata aaaaataatg aaagattttt ataaaataat aaaatacatt
     4801 ttcccgtata aggtacaggc tattttaaac atttttttca atgttctttc aacaatattc
     4861 tctgtttttt catttgctat tgtaattcct tttttaggaa ttttgttcaa aactcaagca
     4921 ttagttactg aaaaacctgc attagacatt actagcgttt ctagtttaca acataacttc
     4981 aattattggt taagcaatat tattattgaa catggcgaaa taaaagctct gataagcatg
     5041 ggtgtattag ttattattgc tacttttttt aaaactctat gcttttattt ggcaaatcac
     5101 tttatggttc cacttcgcaa tggagttgta aaagacattc gcaatcagct attcgacaaa
     5161 gttataagtt taccaatagg atatttttca gaagaaaaaa agggaaatat tattgctaga
     5221 atgacaaacg atgtgcaaga aattgaatgg ggaataatga gctctttaga aatgattttc
     5281 agagacccaa ttaaaatctt aatctatctt ggcacaatgt ttatcattag ttggcaaatg
     5341 acaagctttg tgtttatttt acttcctatt gttggtctaa ttattggacg aacaggacgt
     5401 agcttacgaa aagtttcaat gacagggcaa caacaaatgg gaagtttgat ggctcaaatc
     5461 gaagaaatga ttggtggctt gagaattatc aaagctttta atgcagagaa acatatttcc
     5521 gacaaattta aagacacaaa taaagactac actggcttga tgaactttgt gaatagaaaa
     5581 agatttttag caagtccatt aagtgaattt ttagctacta ttgctgtggt tgttataatg
     5641 tggtatggcg gaacattaat tttaggcgac aatgcattgc tctcgcctgc tgcattaatt
     5701 ggttatttag ttatttttag tcaaataatt tcgcctgcta aaaccttgtc atcttcgtgg
     5761 tttaatatta aaaaagccat ggcttctgtt gatagagtta atgatatttt agaccaaaca
     5821 aatcctatag ttgaaaaaca aggtgctaca gaaattaccg aatttaaaaa cagtatagaa
     5881 tttaaaaatg taaacttcgc atacaccgaa gatattcctg ttcttaaaaa tataaattta
     5941 aaaatacaga aaggacaaac cgttgcaata gttggtcaaa gcggttctgg aaaatcgaca
     6001 atggttgatt taattccaag attttatgat attttagaag gcgaaatttt aatagacgga
     6061 attaatatta aagattataa actttcaaat ttgcgagaac taataggcta tgtacaccag
     6121 actccaatac tttttaatga tagtattgca aataatattg cttttggaaa acaaaatgta
     6181 tcacaaaaaa atatagaaga agccgccaaa gtagcaaatg ctcatgattt tattttagaa
     6241 agcgaaaatc aatatgatac aaatataggc gatggtggtt gtaaactatc tggtggtcaa
     6301 cgtcaaagat tgagtattgc gagagcagtt ttaaaaaatc ctcctatttt aattttagac
     6361 gaagctacat ctgctttaga cacagaatcg gaaaatttgg tgcaagaagc tttggataat
     6421 ttaatgcaaa atagaacttc aatagttatt gcccacagat tgtccacaat aaaacgtgct
     6481 gatttaattt gcgttcttca cgaaggacaa attgtagaaa caggaaaaca cgaagaactt
     6541 attgcaaaaa aaggaatgta ttataaactt aacaatattc aaaacggata atctttcaag
     6601 aaaaacaatt atatttgcac taaaataaaa cttctatgaa aagaaaatta atatattttt
     6661 caataatctg cacactttta gttgcatgtg gttctaatga taagccaaaa agacccgatg
     6721 gaatggcaaa aaacactgga gaattaaaat ttcaagatta taattttgaa aatcctccac
     6781 aatttagaaa agacggagat ttaagatttt atgacagcaa tactcaagaa gaattatttc
     6841 atatagaaac agaagtagca agcactgatg agtcaagagc tagaggttta atgtttagga
     6901 aagaaatgct cgaaaatcaa ggaatgtttt tcttatttcc aaaagatgaa attcaagctt
     6961 tttacatgag aaacacttta attcctttag atatacttta tgtaaactca aaaatggaaa
     7021 ttgttgatat ttataaaaac acaaaaccta aagacgagac atcgctacct tctaaagagc
     7081 ctgctaaata tgtaattgaa ataaatgccg ggctttgcga caaatataat atcgaaatag
     7141 gcgattatat tgtatttgat taatcctttg tgtgagtaaa tttcccgttt agcttaacta
     7201 ttccaataag tggcgaatct aaattttcta cattcaaatt aaacactccg caaatattat
     7261 ctttgtcaac ataatcaata gtaatacctc cttgagcagg cattccagac aaccaattaa
     7321 aataaacttc gccaaaacat gtaacaatat ttacctgaca ccacaagcca gattgataat
     7381 ctaaataagc ataataaaca tcgctaattt ttttaccttg tctagcattt atgccaataa
     7441 gtatatcgta ttgattttca gcaagtcgcc cattaaattc ctcttcagaa taattcttta
     7501 atttcaaact gatagttgta tcatttatcc attccatttc agattgtttt acctcaaact
     7561 tatagttttc aacaactaaa gtatccgaca tagaataata ataatcagca attgtcaatt
     7621 cgatatcatt ctcgtaatca caaggcttaa tttcttcctc agcttcattt ttttttgttg
     7681 actgcgaaca tgaaaatagt gctaacacaa tagcaaatag taaaatagta gttttgaatt
     7741 tcattagttt aagtttatag ttaaaagatt atgcaagata atacattttt aaaaatctcg
     7801 tctaacaaag tgaatatatt ttacaaaaca caaataatca aagacttaca aacacaaaaa
     7861 ttatacaaac aactgtg
//
LOCUS       DAOPNX010000107         7861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_10338_length_7861_cov_3.602229, whole genome shotgun sequence.
ACCESSION   DAOPNX010000107 DAOPNX010000000
VERSION     DAOPNX010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7861
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10338_length_7861_cov_3.602229"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PKN53_09750"
     CDS             <1..695
                     /locus_tag="PKN53_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89272.1"
                     /translation="ATKPIKELLKTIIHLENSELDRDKRKLLSDLYKEQYYILDNQRN
                     TINSRYDVQAKNFHDIIISEVDDFGLEVYPSMYTPAFKTFDILIGEKTDKMIDLTNIA
                     HLSELYKNWHEYPMLHETLEFFEGLLYLSKKKQLVLKTRKTGMPQISAAKYIERELGS
                     SINPNYLSTILYKQILPAFAEKVQDFINSQGDELFQCVECGKNLPDNSKFYHYSNGKR
                     KRKKCKKCYLGD"
     gene            697..1644
                     /locus_tag="PKN53_09755"
     CDS             697..1644
                     /locus_tag="PKN53_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89273.1"
                     /translation="MKKKCTYCKKEKTLDVFIDTENIFFVDKKFPICLECLNDFFTQK
                     VPSGKEWDTFNKFCQTIDIYFDPQLYQELRDIDAENALILYMKKAPKADTVDWNTLNR
                     AYLKLVTLGEIHQVHPVLEEGELLRLKDAWGEEYLQEECLELEKMLKSWRQTQAISNH
                     NQLEDTKRLCKIALQMDKKVRAGEDIDKVLKTYVALKDSLGFSNKSKGNSDSIETVGE
                     MFSYLEKSGWLNTYYQGEENDELDKTILEMQAWIKRVVIDNSEIGDQLEATLKQVVTL
                     LNKSNKYEELPSDEDIDEAMEQEDKELVNFFNEGEISDD"
     gene            1637..1822
                     /locus_tag="PKN53_09760"
     CDS             1637..1822
                     /locus_tag="PKN53_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89274.1"
                     /translation="MIDYKRILAKDTSLTCAKKNGVVFEKGYSIDKEWLERNIEKVRH
                     TFNSSTWEGTSNTINKR"
     gene            1938..3188
                     /locus_tag="PKN53_09765"
     CDS             1938..3188
                     /locus_tag="PKN53_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89275.1"
                     /translation="MVAALDSTRGRRKHGGIIDEVRDHDGDKLNNIILPLFNVSRRTL
                     LGKLNPYEKKQRIIYITSAGDKFSFAYDKMIETLIQSIISPQDAWVFGCSYKVPLMHN
                     LLDENYLNQIKTSTTYSAESFAKEYLGRWLAGGSDSWFDGELLSKRRIVKTAEHYYHQ
                     SQNKKAFYLIAVDVAKYSCQTVASVFKVLPKDGKYYSKLVNIKILGLTEDRKHFEKQA
                     IDLKKMIADYRVKEIVIDGNGIGDGLIDFMIIENSEDGVIYPAYGVKNDTKGHYKTAN
                     KYAGEKIIYNIKGNDELNSKIYSNALSRIQSGQVAFLISETLEKARIQNSSSYKKIEK
                     RIEHLLPYELTTRLFAEMLNLKLSPRTRATNLIVLEQKSKSTLKDKFSSASYGLWRIK
                     ELEDEYLNNLKKSRNSARNLIFVD"
     gene            3224..4660
                     /locus_tag="PKN53_09770"
     CDS             3224..4660
                     /locus_tag="PKN53_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89276.1"
                     /translation="MNTNYEKFSKELSGFLSGMENAVISTYGNERYAPEETYEYTKEE
                     IIRIIKEGDSLERSKLSLTFYRVNGLYSALIHHYSNFLLYLYVLSVKPSAKKMPFSKY
                     EKEYYQALNYLEDSDMYNLSMKIFNEVLVKGGYYVLATKTNTSVVLQDLPFEYCRCRF
                     LDLNGYDVVELNLEYFNSVRNQIDRELLLNTYPKEIVKEYNKYVRGKRDRWLPLSTKY
                     AFYLNIFEEKPFFLNTLLSLIGIEKKKEYQDQFDKKQLGTLITLEPELLKDGDFAIEP
                     NEMKVLHKGAVANVGKPLDAKILSTYAKVNVHKLANDNSAKITNEDILDSFYADAGIS
                     YKLFNADTDKTAEFILQKDLAFCVKFGEKIAKFFERILLLLLNTKKVEFSVNILPVAQ
                     FNKEEEFKKSKELLAFGYSFLIPHILAGGKQSNLLSLKQLENDGLDLDSVLKPLMSSY
                     TTSGKNLPQDKEKVKEEEKPVEQEENQL"
     gene            4635..5873
                     /locus_tag="PKN53_09775"
     CDS             4635..5873
                     /locus_tag="PKN53_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89277.1"
                     /translation="MNKKKINYNLPIEFSKNLEKVSSNLSKCRARICYGDRNRNYTCF
                     EDVLPQMEKSLIYTPIKAIWSDEKRDFEGHGWNSTQGRIYGIVPADQTITYEENSDED
                     GTIKKYMACDVYLYTGLYTEAGYIAGKGLSLELNPNTAKGKWIEDENGDSYFKFSQAE
                     FQALQVLGNDVEPCFGGAAFYEFLMTQKKEDNDKMEIKKIAENLYELVDGEVKTLLYT
                     VTKEEADKLGVATVSSVYEQMDELQAKYSTLETEKAEVAEKYSTLETEKVEIETKFSA
                     LEAEKIVLDEKYSVLETEKVEIENKYSTLQVEKVEIETKFSALEAERLEKETLEKNSL
                     IEKYSKIIGEEKAVELEDIKSKVTQYSVQEIEKELAFLYTKSNEGVLFEGQRIYSHGG
                     SHKYENPDKTLELIKKVGGK"
     gene            5876..6490
                     /locus_tag="PKN53_09780"
     CDS             5876..6490
                     /locus_tag="PKN53_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89278.1"
                     /translation="MALKRLTIEGYGVAEWNKIAAPFTAKMESQLPLDPDVFKANDSD
                     TSGIFCEVGQFLALDKTNMVATVPTTATDADYLPIGYNFSSEVLYDERKRGLKNFKQT
                     CEDFLPNIGIPEVSDVITINAVLYDDTEFAAASPKTAEDVFIEALENVATTPLYGILT
                     NASDGFLVITGTVANNLGGIVYKVVEKTSMPDGSTGIKLQAVKA"
     gene            6604..7551
                     /locus_tag="PKN53_09785"
     CDS             6604..7551
                     /locus_tag="PKN53_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89279.1"
                     /translation="MVLEDVNKTLAAEIAKYTSDYYDLQDNKNLIYRLIGETFDIAVP
                     KRVLPYFENFATTKVVKANDRKYFKIIKGKLRAKQFVTAVGLSGRYETFRLDTDEFEV
                     KTTAIGGACRVNFERWANGDESLSDYTEVLMDGIIDRMHGEVQKALQTAINATNRPSA
                     NKISATGFSADNMLKLCNVAKSYGDSAVIYATPEFIAAMGPDEIAQNVYSQKQVDDYV
                     ATGKIKSFKGFPIVEIPQSFIDENNAVTVVNPQFAYIFPIGKTKPVASVIEGKTIVKE
                     FQGHDSSTEVEAYLRFGVSVLTNHDWCIYQNTGITDTSR"
     gene            7611..>7861
                     /locus_tag="PKN53_09790"
     CDS             7611..>7861
                     /locus_tag="PKN53_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89280.1"
                     /translation="MDKVFITSQVSGTILLIDSEAKVNRTFKKKGQKHFVTADQWDII
                     QFNEGFNYMLNQGMLYVETPEAKKDTPIEEFEKIDENLI"
BASE COUNT         3171 a         1005 c         1420 g         2265 t
ORIGIN      
        1 aggcaacaaa acctataaaa gaattgttaa aaacaattat acatttggaa aatagtgaat
       61 tagataggga taaaagaaaa ttgttaagtg atttatataa agaacaatat tatatattgg
      121 ataatcaaag aaatactata aatagtagat atgatgtgca agcaaaaaac tttcatgata
      181 ttattatatc tgaagtagat gattttgggt tagaggtata tccaagtatg tacacacctg
      241 catttaaaac ctttgatatt cttataggtg aaaaaaccga taaaatgata gacttaacaa
      301 acattgcaca tttaagtgaa ttgtataaaa attggcatga atatcctatg ttgcatgaaa
      361 cattagaatt ttttgaaggt ctattgtatc tatcaaaaaa gaaacaattg gttttaaaaa
      421 caaggaaaac gggaatgccg caaatttctg ctgctaaata tatagaaaga gaattaggtt
      481 cttcaataaa cccaaattat ttatcaacta ttctatataa acaaatactt cctgcatttg
      541 cagaaaaagt tcaagatttt attaattcac aaggtgatga acttttccaa tgtgttgaat
      601 gcggcaaaaa tttgcccgat aattctaaat tctatcacta ttcaaatggc aaaagaaaaa
      661 gaaaaaaatg taaaaaatgt tatttaggag attaatatga agaaaaaatg tacttattgc
      721 aaaaaggaaa aaacactaga tgtttttata gataccgaaa atatattttt tgtagacaaa
      781 aaatttccca tatgtttaga gtgtttaaac gatttcttta ctcaaaaagt accaagtggc
      841 aaagagtggg atacttttaa taaattttgt caaactatag atatatattt tgacccacaa
      901 ctttaccaag aacttagaga tatagatgct gaaaatgcac ttatattata tatgaaaaaa
      961 gccccaaaag ctgatacagt tgattggaat acattaaata gggcatattt aaaattagtt
     1021 acacttggcg aaattcatca agtacatcct gtattagaag agggcgaatt attaagactt
     1081 aaagacgctt ggggcgaaga atatttacaa gaagaatgtt tagaattaga aaaaatgcta
     1141 aaatcttggc gacaaactca agcaatatcc aatcataatc aattagaaga tacaaaaaga
     1201 ttatgtaaga ttgctttaca aatggacaaa aaggttcgag caggtgagga tattgataaa
     1261 gtactaaaaa catatgtagc tttaaaagat agtttgggct tttccaacaa atcaaaagga
     1321 aactctgata gtattgagac agttggcgaa atgtttagtt atttagaaaa atcaggttgg
     1381 ttaaatacct attatcaagg cgaagaaaat gatgaattag ataaaacgat attagaaatg
     1441 caagcttgga taaaaagggt tgtaattgat aattctgaaa ttggcgatca actcgaagca
     1501 acattaaaac aagttgtaac actattaaat aaatcgaata aatatgagga attaccatct
     1561 gatgaagata ttgatgaggc aatggaacaa gaggataaag agctggtaaa cttctttaac
     1621 gaaggtgaaa taagcgatga ttgattataa aagaatattg gcaaaagata cttcattaac
     1681 ttgtgcaaaa aagaatggtg ttgtttttga aaaaggctac tctattgata aagaatggtt
     1741 agaaagaaat atagaaaagg tgcgacatac tttcaatagt agcacctggg aaggaacaag
     1801 caacacaatt aacaaaagat aaatttaatg agatatttgg attatttcca ctactaaaaa
     1861 aagaattagt aggcggagat tttttttcag gtaaagatta tatcaatgct atattagaaa
     1921 acggctcaac tttagaagtg gttgccgctc tcgactctac tcgtggacgt agaaaacatg
     1981 gtggaattat agatgaggtt agagaccatg atggtgacaa attaaataac ataattttgc
     2041 cgctttttaa tgtaagtaga agaacactac ttggtaaatt aaatccttat gaaaaaaagc
     2101 aaagaattat atacataact tctgcaggtg ataagttttc atttgcatat gataaaatga
     2161 tagaaacact tatacaatct attatatcgc cacaagatgc ttgggtattt ggatgtagtt
     2221 acaaagtgcc acttatgcac aaccttttag atgaaaatta tctaaaccag attaaaacat
     2281 caactactta ctctgctgag tcttttgcca aagaatattt aggtagatgg ttggcaggtg
     2341 gaagtgatag ctggtttgac ggtgaactat taagtaaaag aagaatagtt aaaacggctg
     2401 aacattatta tcaccagtca caaaataaaa aggcttttta tttaatagca gttgacgttg
     2461 caaaatatag ctgtcaaaca gtagcctcag tttttaaggt attaccgaaa gacggcaaat
     2521 attatagtaa gttagtaaat ataaaaatat tgggcttaac tgaagataga aagcattttg
     2581 aaaaacaagc aatagactta aaaaagatga tagctgatta tagagtaaaa gaaattgtta
     2641 tagatggaaa tggtataggt gacggactta tagattttat gataatagaa aattctgaag
     2701 atggtgttat ttatccagca tatggcgtaa aaaatgacac aaaaggacat tacaaaacag
     2761 ctaacaaata tgcaggggaa aaaattattt ataatataaa aggtaatgac gaactaaatt
     2821 caaaaattta ttctaatgct ctatcaagaa tacaaagcgg acaagtagct tttttaatta
     2881 gtgagacttt agaaaaagct agaatacaaa attcttcttc atataaaaag atagagaaaa
     2941 gaatagaaca tcttttacct tatgaattaa cgacaagact atttgccgaa atgttgaacc
     3001 taaaactttc gccaagaaca agagcaacca atcttattgt gctagaacaa aaaagtaagt
     3061 caacattgaa agataaattt tcgtcagcat cttacggatt gtggcgaata aaagaactag
     3121 aagatgaata tttgaacaat ttgaaaaaga gcagaaattc tgcacgaaat cttatatttg
     3181 ttgattgata aacgtacttt atatataagg ggtgataaaa acaatgaaca ctaactatga
     3241 aaaatttagc aaagagctaa gtggctttct atctggaatg gaaaatgctg ttatttcaac
     3301 atatggaaat gaacgatatg cacctgaaga aacgtatgaa tatacaaaag aagaaattat
     3361 tagaattatc aaagaaggcg atagtttaga aagaagtaaa ttatcactca ctttttatag
     3421 agtaaatgga ttgtatagtg cattaataca tcattattca aactttttac tttatttgta
     3481 tgttttaagt gttaagccta gtgccaaaaa aatgcctttc agtaagtatg aaaaagagta
     3541 ttatcaagca ttaaattatt tagaagatag cgatatgtat aatctatcta tgaagatatt
     3601 taatgaagta ctagtaaaag gtggctatta tgtattggca acaaaaacaa atacatccgt
     3661 tgtattacaa gacttacctt ttgaatattg tcgttgccgt ttcttagatt taaatggtta
     3721 tgatgtggta gaacttaatt tagaatactt caattcggtg agaaaccaaa ttgatagaga
     3781 actcttatta aatacatacc ctaaagagat agtaaaagaa tataataaat atgtaagagg
     3841 caaaagagat agatggttac cactatctac aaaatacgct ttttatttaa atatatttga
     3901 agaaaagcct ttctttctta atacactctt atctttgata ggtattgaga aaaagaaaga
     3961 atatcaagac caatttgata aaaaacaatt aggaactcta ataacattag agcctgaatt
     4021 gttaaaagac ggtgattttg ctattgaacc aaatgaaatg aaagtattac ataaaggagc
     4081 agtagccaat gtaggaaaac cacttgatgc aaaaatacta tccacttatg caaaagtaaa
     4141 tgtacacaaa ttggcaaatg ataattctgc aaaaataaca aatgaagata ttttagatag
     4201 tttttacgcc gatgctggaa tatcttataa attgtttaat gcagatacag ataagacagc
     4261 tgagtttatt ttacagaaag atttagcctt ttgtgttaaa tttggcgaaa aaattgccaa
     4321 attctttgaa agaatactct tgttattgtt aaatactaag aaagtagaat tttcagtaaa
     4381 tattcttcct gttgcacagt ttaacaaaga ggaagaattt aaaaaatcaa aagaattgtt
     4441 ggcatttggc tatagctttt taattccaca tattttggca ggtggaaagc aaagcaatct
     4501 attatcatta aaacaattag aaaatgatgg tcttgattta gacagtgtgt taaaaccact
     4561 aatgtcatct tatacaacaa gtggcaaaaa tttaccacaa gataaagaaa aagttaaaga
     4621 ggaggaaaag ccagttgaac aagaagaaaa tcaattataa tttacctata gaattttcta
     4681 aaaatttaga aaaggtaagt tctaatttaa gcaagtgccg agcaagaatt tgttatggcg
     4741 acagaaatcg taactatacc tgttttgagg atgttttgcc tcaaatggaa aaaagtttaa
     4801 tatatactcc aattaaggca atttggtcag atgaaaaaag agattttgag ggtcatggct
     4861 ggaactcaac tcaagggcga atttatggta ttgtgcccgc agaccaaact atcacatatg
     4921 aagaaaattc agatgaggat ggtacaataa aaaaatatat ggcttgtgat gtatacttat
     4981 acacaggttt atatacagag gcaggctata tagctggcaa aggcttgtct ttagaactta
     5041 atccaaatac tgcaaaaggt aaatggattg aggatgaaaa tggtgatagc tattttaaat
     5101 ttagccaagc agaatttcaa gcactacagg ttttaggtaa tgatgtagaa ccttgctttg
     5161 gcggggcggc gttctatgag ttcttaatga cacaaaaaaa ggaggataat gataaaatgg
     5221 aaattaaaaa gattgccgaa aatctttatg aattagttga tggcgaagta aaaactttgt
     5281 tatatactgt tacaaaagaa gaggcagaca aattgggtgt ggctactgta agttcagttt
     5341 atgaacaaat ggacgaacta caagcaaaat attcaacttt agaaacagaa aaagctgaag
     5401 tagcagaaaa atattccaca ttagaaactg aaaaagtaga aatagaaact aaattttcag
     5461 cacttgaggc agaaaaaatt gttcttgatg aaaaatattc agttttagag acagaaaaag
     5521 tagagattga aaacaaatat tctactttac aagtagaaaa ggtagaaatt gaaactaaat
     5581 tttcagcact agaggcagaa agattagaaa aagaaacctt ggaaaaaaat tcactaattg
     5641 aaaaatactc taaaattata ggtgaagaaa aagccgtaga attagaggat ataaagtcaa
     5701 aagtgactca atattcagta caagaaattg agaaagagtt ggctttctta tacactaagt
     5761 caaatgaggg ggtattgttt gaaggtcaaa gaatatactc acatggtggt tctcataaat
     5821 atgaaaaccc agataaaaca ttagaattaa ttaaaaaagt aggaggtaaa taataatggc
     5881 attaaaaaga ctaacaatag aaggctatgg cgtagctgaa tggaacaaaa ttgcagctcc
     5941 atttacagct aagatggaat ctcaacttcc attagaccca gacgttttca aagcaaatga
     6001 ctcagatact tcaggtatat tttgtgaagt aggacaattc ctagcattag acaaaacaaa
     6061 catggtggca actgttccaa caactgctac tgacgcagat tatcttccaa taggatataa
     6121 cttctcaagt gaagtattat atgacgaaag aaaaagaggt ttaaagaatt tcaaacaaac
     6181 ttgtgaagat ttcttaccta acataggtat accagaagtt tcagatgtta taacaataaa
     6241 cgctgtatta tatgacgaca cagagttcgc tgcagcttca cctaaaacag ctgaagatgt
     6301 atttattgaa gctttggaga atgtagcaac tactccacta tatggtatat taacaaacgc
     6361 atccgatggt tttctagtaa ttactggtac agttgctaat aatttaggtg gaatagttta
     6421 caaagttgta gaaaaaacat caatgccaga tgggtctact ggtataaaat tgcaagccgt
     6481 gaaggcctaa ggaggggtat aataatgaaa ttaaaacaag gaacaactga gtatcaaaca
     6541 ctaaaagatt taatgttata ctcagctaaa aatgaagtac caactaacta tactgtagaa
     6601 ggtgtggtat tagaggatgt aaataagaca cttgccgctg aaatagctaa atatacaagt
     6661 gactactatg atttacaaga caataaaaat ctaatctatc gtttgatagg tgaaacattt
     6721 gatatagcag taccaaaaag agtattacct tattttgaaa attttgctac aactaaagta
     6781 gtaaaagcaa atgacagaaa atactttaaa ataatcaaag gaaaattaag agcgaaacaa
     6841 ttcgttacag cagtaggttt gtcaggcaga tatgaaacat tcagactaga taccgacgaa
     6901 tttgaagtaa agacaactgc tatcggcggt gcttgtagag ttaacttcga gagatgggca
     6961 aatggtgatg aaagtctatc agattacact gaagttttaa tggatggtat tattgataga
     7021 atgcacggtg aagtacaaaa agcacttcaa actgctatta atgctacaaa tagaccatca
     7081 gctaataaaa tttcagcgac tgggttctca gcagataata tgttgaaact ttgtaatgtt
     7141 gcaaaaagct atggcgactc agctgtaatt tatgctacac cagaatttat cgcagcaatg
     7201 ggtcctgatg aaattgcgca aaacgtttac tcacaaaagc aagttgatga ctatgtagct
     7261 acaggaaaaa ttaaatcatt taaaggtttc ccaattgtag aaatacctca atcatttata
     7321 gatgaaaata atgcagttac tgtagtaaat ccacagttcg cttacatttt cccaattggc
     7381 aaaacaaagc cagttgcttc agttattgaa gggaaaacaa ttgttaaaga gttccaagga
     7441 catgatagtt ctactgaggt agaagcttac ttacgttttg gcgtttctgt tctaacaaac
     7501 catgactggt gtatatatca aaatactggc atcacagaca catctagata attaaaataa
     7561 aatatatgag ggggtaacac ccccttgagt taaaaaagga gaataaaaaa atggataaag
     7621 tttttataac ctcacaggta agtggaacaa ttttgttaat agactctgag gctaaggtta
     7681 atagaacctt taaaaagaaa ggacaaaaac attttgtaac agcagaccaa tgggatatta
     7741 tacagtttaa tgaagggttt aattatatgt taaatcaagg aatgttatat gtagaaacac
     7801 ctgaggcaaa gaaagatact ccgattgagg agtttgaaaa aattgacgaa aatcttataa
     7861 a
//
LOCUS       DAOPNX010000108         7793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_10447_length_7793_cov_6.074696, whole genome shotgun sequence.
ACCESSION   DAOPNX010000108 DAOPNX010000000
VERSION     DAOPNX010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7793
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10447_length_7793_cov_6.074696"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1342)
                     /locus_tag="PKN53_09795"
     CDS             complement(80..1342)
                     /locus_tag="PKN53_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOD89281.1"
                     /translation="MEWIIVGIMIVLSAFFSGSEIAFVSSNKLRLELASKESSLSSRI
                     LTIFNSYPSRYITTMLVGNNIVNTIYGIFMARILYEPIIQHVNSEFYVLLIQTIVSTA
                     VILVFAEFIPKAFFRIINYTAVKAFSIPLIICYYLLYPIGSLTIWISSLLIRIFTGKK
                     IDFSTKQLAFSKIDVEEMLEAGNFHEADNQNQQELKFFKKAIDFSSIKLRECIIPRNE
                     IVAVPSSIGMTDLRDKFIESGHSNILVYKQSIDEIESYINVKDIFKNPKSIEEISRDI
                     LIVPETMSAKKLFDKLIQSNKSLAVVVDEFGATSGMVTLEDILEEIFGEFEDEHDFPE
                     PDVIINSDGNYIMDGRQEVDLFNHEYGFDLSESDEYETIAGYILHHTGNFPKQGTVVT
                     VNEDKKEYKFKILKVQDTKIEKVMLINK"
     gene            complement(1345..1557)
                     /locus_tag="PKN53_09800"
     CDS             complement(1345..1557)
                     /locus_tag="PKN53_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89282.1"
                     /translation="MNKNRRIVMIVIEVLYIITAMFCVGVGIYYHIKHGFDKSWLFYG
                     IGIISLGMFGVRRIQRRNLERRESRK"
     gene            complement(1550..2116)
                     /gene="lptC"
                     /locus_tag="PKN53_09805"
     CDS             complement(1550..2116)
                     /gene="lptC"
                     /locus_tag="PKN53_09805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOD89283.1"
                     /translation="MNSKFIPLTIIIVSILVFSTVFTACKNDIDTINKITEVQDLPTF
                     DLTGLETVYSDSGIIKLKIYAPKLVRYEKREIPCDIYEKGLFVEFYNRSMLVSGTLRC
                     NYAKYSIREEIWEAKSNVEINNSDNKEKINTEQLFWDMKAEKIYSDKFVRITTEDEIL
                     YGEGFESNQEFTNWKIIKPTGSIKIKDE"
     gene            complement(2109..3440)
                     /locus_tag="PKN53_09810"
     CDS             complement(2109..3440)
                     /locus_tag="PKN53_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD89284.1"
                     /translation="MKTFVFFIGILLAVMFSNSAVAQEGDMGSDPDKCMIMLSTYNEF
                     VIQQNYKDAMTAWRWCFINCPEATRNIYIHGTTIVEYFIEQQTEDAAREAYIDTLMMV
                     FDNRIKYYNQEALVLGRKGISMLKYRNEQVKEANEVLRRSFELGRESSEYFVLQYLMQ
                     TTAILYSNELTTQEDVVNTYSDLSDVINTQIDNEASESKRARLEETAKNIEEIFVNTG
                     VADCPTVIRLFGPKFEANPEDISLALKILALLDRGQTDDCKLSDLYMKCAIAAYNAEK
                     TSTSAHSIAQSYFKRGDATNAEKFYEEAISLEENSLKKADMYYELALLYYSNFKNYVK
                     ARQAARNALVNNPNYGKAYILIGRLYAAGGSCGETVVEKKSLNCLIVDQFIKAKNVDP
                     SVSSEANELIYRYSAAFPTGDELFWENYKAGQTITIGCWIGETTTLRAKSE"
     gene            complement(3462..4739)
                     /locus_tag="PKN53_09815"
     CDS             complement(3462..4739)
                     /locus_tag="PKN53_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89285.1"
                     /translation="MHKRIILILALTISSIICLAQGRVGSPYTRYGLGDVNQNSLIRN
                     NAMGRTSYTLPYNNGINFINPALVSQIDTMTFIFDFGFNGGIRTYKTETQGSMSNYDF
                     QISHLIFGFPITKWWKSCVGFMPYSNVAYSIVDRDSSLNVAKDYIYSGNGGLNQVAWS
                     NGFTPFKNFSIGINAFYYFGKIYHSNSIDFDDKTGAFLNVIEQNTVRINDFSFDLGMN
                     YNINLNPENKLSISGVYAYNSQLNAVRNTMVFNTLSHSSAITDTIFQSINEKGKISLA
                     QKIGVGIGYNHDDKLYVGVDYTIQDWSKAKYFELSDQLSNSTFISVGTEYTPAGRGKL
                     YYKYWQGVSYRAGFYSNQNFINLNNSETKISDFGISFGLGLPTKRSKTSYNLSLQIGQ
                     RGSAKNNFITENYFIIGFSLNLADMWFIKSKFD"
     gene            complement(4732..5463)
                     /locus_tag="PKN53_09820"
     CDS             complement(4732..5463)
                     /locus_tag="PKN53_09820"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOD89286.1"
                     /translation="MFNLIIDAGNTIIKLRVLMQKEIVSTENCKDWDSALNSIKLLQK
                     NFKISACILSNVRESNALFENYLTKNFKTIFFSHTNKLPVIINYKSLETLGIDRLAAV
                     CGASELFPNKNSLIIDAGTAITYDILKDGKIYEGGNISPGIKIRFKALNTFTKKLPLI
                     DIDAEDNNLFGQNTEQAIRYGVQNGILHEVKGVIEDFKLKYSDLNIIFTGGDSFFFEK
                     LLNFRIFAEPNLTTLGLNKILELNA"
     gene            5691..6644
                     /gene="arcC"
                     /locus_tag="PKN53_09825"
     CDS             5691..6644
                     /gene="arcC"
                     /locus_tag="PKN53_09825"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOD89287.1"
                     /translation="MENTKLAVVAFGGNALLRAGQKGRVEEQAANVYTTCESLLSLVK
                     NGYNLVVSHGNGPQVGNILLQVDAGEKLYNIPEMPLDICGAYSQGFIGYLIEQQFKNV
                     LVKNGLDKNIITLVTQVLVDENDKAFQNPTKPIGPYYTEEEKDALKLKNPQDCFAKDP
                     KGKGWRKIVASPKPIDISNKEIVERLSREGNIVVAVGGGGIPVFYPEKGRLQGIDAVI
                     DKDLASSMLAVYINADEFYILTDVPKVCLNFNTASEKRLDTITVSEAKQYLEQGHFTE
                     GSMAPKIRAALNFVENGGKVCIITEASQLGQPMSGTRVVRG"
     gene            6789..7142
                     /locus_tag="PKN53_09830"
     CDS             6789..7142
                     /locus_tag="PKN53_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89288.1"
                     /translation="MRSVLFLLFVLTISCRAISQTNNYTLVKVIDSVLNNYHYIEIKQ
                     DTNIYQAICRFDIITLDSLCIGLQYIIEPKQHAFVEDIDSLTPFEKVNSHYIDNKLIF
                     KLEDIIVIKSMKRFE"
     gene            7267..7551
                     /locus_tag="PKN53_09835"
     CDS             7267..7551
                     /locus_tag="PKN53_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89289.1"
                     /translation="MRFYKIFIFGLLILLFFESNSQNIYDNLGVLDTVEGVCKIRKIK
                     KSKNKTGYAIYAEAEIKGGKARFVIVSLMANGKIKNKDIKKIKRNKHYYF"
BASE COUNT         2626 a         1170 c         1131 g         2866 t
ORIGIN      
        1 ttaaacaaaa taaataaatg acaaaattaa aattttgaat ttaacgaata tcccttcttt
       61 attatttggg aattgataac tatttattta tcaacattac tttttcaatt ttggtgtctt
      121 gaactttaag gattttaaat ttatattctt ttttatcctc atttacagta acaacagttc
      181 cttgtttagg aaaattacca gtatgatgta aaatatatcc agcaatggtt tcatattcgt
      241 ctgattcgct taaatcaaaa ccatattcat gattaaataa atcaacttct tgtcttccgt
      301 ccataatata attaccatca gaatttatta ttacatcagg ttctgggaag tcgtgttcgt
      361 cttcaaattc gccaaaaatt tcttctaaaa tatcttctaa agttaccatt ccgctggtag
      421 caccaaactc atcaacaaca acagctaaag atttattgct ttgaataagt ttgtcgaaaa
      481 gtttttttgc cgacattgtt tctggaacaa ttaaaatatc tcttgaaatt tcttctatgc
      541 ttttaggatt tttaaaaatg tctttaacat taatatatga ttcaatttca tcaatacttt
      601 gtttatagac taagatattt gaatgtccag actctataaa tttgtctcgc aaatctgtca
      661 ttccaatact tgaaggaacg gcaacaattt catttcttgg aattatacat tctcgaagtt
      721 ttattgatga aaaatcaata gcttttttga aaaattttaa ttcttgttgg ttttggttat
      781 ctgcttcgtg aaaattaccg gcttctaaca tttcttctac atcaattttt gaaaaagcta
      841 attgttttgt ggaaaaatca atttttttgc ctgtaaatat tcttattaaa agcgaagaaa
      901 tccaaattgt taaactgcct ataggatata ataagtaata acaaataatc aaaggtatag
      961 aaaaagcttt tacagccgta taatttatta tcctaaagaa tgcttttgga ataaattcgg
     1021 caaaaactaa aatcacagct gttgacacaa ttgtttgtat caatagaaca taaaattcag
     1081 aatttacatg ttgtatgatg ggttcatata gaattctagc cataaaaata ccataaatag
     1141 tatttactat attgttcccg accaacattg ttgtgatata acgcgaagga tatgagttaa
     1201 aaatagttag tattctagaa cttagactag attcttttga tgcaagttct aatcttaact
     1261 tatttgaaga tacaaacgca atttctgaac ctgaaaaaaa ggcagacaaa actatcatta
     1321 tgcctacaat aatccactcc ataattattt tctactttct cttctttcta agtttcttct
     1381 ttgtatgcgg cgaaccccaa acattcctaa tgaaataatt cctattccat aaaataacca
     1441 tgatttgtca aaaccgtgtt ttatgtggta gtaaataccc acgccaacac aaaacattgc
     1501 tgtaattatg taaagaactt caattacaat cattacaatt cttctatttt tattcatctt
     1561 taatttttat tgatcctgta ggttttataa ttttccaatt tgtgaattct tgatttgact
     1621 caaaaccttc accatataaa atttcatctt cagttgttat tctaacaaat ttatcagaat
     1681 atattttttc ggctttcatg tcccaaaaca actgttcagt atttattttt tccttattat
     1741 cgctattgtt aatttctaca ttgcttttgg cttcccatat ttcttctctt atggagtatt
     1801 tagcgtaatt gcatctaaga gttccgctaa ctagcataga gcgattgtaa aactcaacaa
     1861 ataatccttt ttcataaata tcgcaaggaa tttctcgttt ttcgtaccta actaattttg
     1921 gagcataaat ttttagtttt ataatacccg aatcgctata tactgtttct aagccagtga
     1981 ggtcaaacgt aggaaggtct tgaacttctg ttattttatt tatagtgtct atatcgtttt
     2041 tgcaagctgt aaatactgtg ctaaatacta atatagacac tataattatt gttaaaggta
     2101 taaatttact attcactttt agctcttaat gttgtagttt cacctatcca acagcctata
     2161 gtgatagttt gacctgcttt ataattttcc cagaaaagct catcacctgt tgggaaagct
     2221 gctgaatatc tgtaaattag ttcattcgct tcagaagaaa ctgatgggtc aacattttta
     2281 gcttttataa attgatcaac tattaagcaa tttaaacttt tcttttcaac aacagtttca
     2341 ccacaagaac cacctgctgc atatagtctt ccaattaaga tatatgcttt tccgtagtta
     2401 ggattgttta ctaaagcatt gcgtgcagct tgtctagctt taacgtaatt tttaaaatta
     2461 ctgtaatata aaagagctag ctcataatac atatctgctt ttttcaaaga gttttcttct
     2521 aaagaaattg cttcttcgta aaacttttca gcatttgttg catcgcctct tttaaaatag
     2581 ctttgagcaa tagagtgggc tgaagtagaa gttttttctg cattataagc agcgatagca
     2641 catttcatgt ataaatctga aagtttgcaa tcatcagttt gaccacgatc tagcaacgca
     2701 agaattttta acgcaagaga tatatcttca gggtttgctt caaattttgg accaaaaagt
     2761 cttattacag ttggacaatc tgcaacacca gtgtttacaa atatttcttc aatatttttt
     2821 gcagtttctt ctaatcttgc tctttttgat tcgctagcct cattatctat ttgagtattt
     2881 atcacatcag ataagtcaga ataagtatta acaacatctt cttgtgttgt aagttcgttg
     2941 ctatataata ttgcagttgt ttgcattaaa tattgaagaa caaaatattc agaactttct
     3001 cttcctagtt cgaaagatct tcttaatact tcgttagctt cttttacttg ttcgtttcta
     3061 tattttagca tagaaatacc tttacgacct aaaactaaag cttcttggtt gtaatatttt
     3121 attctattat cgaataccat cattaaagta tcgatataag cttctcgagc agcatcttcg
     3181 gtttgttgtt cgataaaata ttcaactatt gttgtgccat gaatataaat gtttcttgtt
     3241 gcttctgggc aatttataaa acaccatctc catgctgtca ttgcatcttt ataattttgt
     3301 tggataacaa attcattata agttgataac ataatcatgc acttgtctgg gtcgcttccc
     3361 atgtcacctt cttgtgccac tgcgctgttg ctaaacataa cagctaataa aattccgata
     3421 aaaaatacaa atgttttcat aataaaaact tttaaaattg tttaatcaaa tttactttta
     3481 ataaaccaca tatctgctaa attaaggctg aatccgatga taaaataatt ttctgtaata
     3541 aaattatttt ttgcacttcc tctttgccca atttgtagcg ataagttata tgatgtctta
     3601 gaacgttttg ttggtaatcc taatccaaaa cttatgccaa agtcagaaat cttagtttct
     3661 gagttgttta ggttgataaa gttttggtta gaataaaagc ccgctctata gcttacgcct
     3721 tgccaatatt tataatataa tttacctctt ccggctggtg tgtattcagt tccaacagag
     3781 ataaatgtac tgtttgataa ttggtcgctt agttcaaaat attttgcttt tgaccaatct
     3841 tgtatagtat aatcaactcc cacataaagt ttgtcatcgt ggttatagcc tattcccaca
     3901 cctatttttt gagctaagct aattttgcct ttttcgttaa tagattgaaa aattgtatct
     3961 gttattgcag aactatgact aagtgtatta aaaaccattg tgtttcgaac agcatttaat
     4021 tgtgagttat aagcatatac tccagaaata ctaagtttat tttcgggatt caaatttatg
     4081 ttgtaattca tacctaagtc aaagctaaaa tcgtttattc ttacagtgtt ttgctcaata
     4141 acatttaaaa aagctcctgt tttgtcatca aagtcaatag agttggaatg gtatatttta
     4201 ccaaaataat aaaacgcatt aattcctata ctaaagtttt tgaaaggggt aaatccattg
     4261 ctccacgcca cttgatttag tccgccattg ccactgtata tataatcttt agctacgtta
     4321 agcgacgaat ctctatcaac aattgaatag gcgacattac tatatggcat aaaacctaca
     4381 catgatttcc accattttgt tattgggaag ccaaatatta aatgtgaaat ttgaaaatcg
     4441 tagtttgaca tacttccttg agtttcagtt ttgtaggtgc gaataccgcc attaaaacca
     4501 aaatcaaaga taaaagtcat agtatctatt tggctaacta aagcgggatt tataaaatta
     4561 atgccattat tatatggtag agtgtagctt gttctaccca ttgcattgtt tctaatcaag
     4621 gaattttggt ttacgtctcc aagtccgtac cttgtataag gcgagcctac tcttccttgt
     4681 gcgagacata ttattgagct tattgttaaa gcaagaataa gaataattct tttatgcatt
     4741 tagttctaaa attttattta atcccaaagt tgttaaattt ggctctgcaa aaatgcgaaa
     4801 atttaataac ttttcaaaga aaaaactatc tccacctgta aaaattatat tcaaatcaga
     4861 atatttcaat ttaaaatctt cgataacgcc tttaacttca tgtaaaattc cgttctgaac
     4921 gccatatcgt atagcttgtt cagtgttttg tccaaataaa ttgttatctt cagcatcaat
     4981 atcaattaac ggtaattttt ttgtaaaagt atttagggct ttaaatctaa ttttaattcc
     5041 aggactaata tttcctcctt cataaatttt accgtctttt aaaatgtcat aagtaatggc
     5101 tgttccagca tctattatta aagaattttt gttaggaaat aattctgaag ctccacaaac
     5161 agcagccaaa cggtctattc caagtgtttc taaactttta taatttatta taacaggcag
     5221 tttatttgtg tgtgaaaaaa atatagtctt aaaattttta gttaaataat tttcaaataa
     5281 agcattgctt tctctaacat ttgataaaat acaggcagaa attttaaagt ttttttgtaa
     5341 aagttttatg ctgtttaatg cagaatccca gtctttacag ttttcggtag aaacaatttc
     5401 tttttgcatt aaaactctaa gttttataat tgtatttcca gcatctatta ttaaattaaa
     5461 catgtctgta tttttattta acaaaaatag aaatttaatt ctcacattta ttagaaagtg
     5521 aagttaaatt atttaaaaaa actttaaaat tggcaaatta gtttagtttt ttgtgtttta
     5581 gcattagttt ttatcaaatt tttgggtgtt gtgaataaaa aagtgaaaaa agttaattta
     5641 tagagaacaa tttttttctt atgtattatt tttgtgagca aaaattagtt atggaaaaca
     5701 caaaattagc agttgtggct tttggcggga atgctctatt gcgagctggt caaaaaggca
     5761 gagttgagga acaagcagca aatgtttata ctacttgcga aagtttgttg agtttagtaa
     5821 aaaatggtta caatcttgtt gtaagccatg gtaatggtcc gcaagttggg aatattttgt
     5881 tgcaagtaga tgctggggaa aaactttata atattcccga aatgcctctt gatatttgtg
     5941 gtgcttattc tcaaggtttt attggttatt tgattgagca gcagtttaaa aatgttttag
     6001 taaaaaatgg attagacaaa aatattatta ctcttgtaac tcaagtttta gttgacgaaa
     6061 acgacaaagc ctttcaaaat ccaacaaaac ctataggtcc ttattatacc gaagaagaaa
     6121 aagatgcttt aaaattaaaa aatcctcaag attgttttgc aaaagaccca aaaggtaagg
     6181 gttggcgtaa aattgtagct tctcccaaac ctattgatat tagcaataaa gaaattgttg
     6241 aaaggctttc tcgcgaagga aatattgtag ttgctgttgg tggtggtgga ataccagttt
     6301 tttatcccga aaaagggcgt ttacaaggaa ttgatgcagt aatagataag gatttagctt
     6361 catctatgtt ggctgtttat ataaatgctg atgagtttta cattttgaca gatgttccaa
     6421 aagtttgctt aaactttaat acagcttctg aaaaaagatt ggatactatt actgttagcg
     6481 aggcaaagca atatttagaa caaggtcatt ttaccgaagg aagcatggca ccaaaaatta
     6541 gagcagcttt aaactttgta gaaaatggcg gtaaagtttg tattataacc gaagcctccc
     6601 aactcggaca gccaatgtcg gggacaagag ttgttagggg gtaggggaag ttctaaacat
     6661 taaatctatc tttcaacaat tttgcttaaa tttgtaaaat aataaggata atttggaatg
     6721 ataacaaaaa ataaaaatcg ttaacatgca aatacaaatt aaaaactatt caaaaaatca
     6781 acaataatat gcgaagtgtt ttatttctat tgtttgtgct tacaataagt tgtcgagcta
     6841 tatctcagac taacaattac actttagtaa aagtgataga ttctgtatta aacaattacc
     6901 attatattga aatcaaacaa gatactaata tttatcaagc gatttgtaga tttgacataa
     6961 tcacattgga tagcctatgc attggtttac aatatataat tgaaccaaaa cagcatgctt
     7021 ttgttgagga tattgactca cttacaccgt ttgagaaagt aaattcacat tatattgaca
     7081 ataaactaat atttaaactt gaagatatta ttgtcattaa atctatgaaa agatttgaat
     7141 aaattatatg gatagcggta gtgtaccata tggagctttt taaaacacaa atttgtaata
     7201 ttttgatagt aaaatgttta tgttagtaaa tctttttata aatttgtaaa aaagaacaac
     7261 gaaataatga ggttttataa aatatttata tttggcttac ttatattatt gttttttgaa
     7321 tcaaattctc aaaatatata tgataatttg ggagttttag acacagtaga aggagtgtgt
     7381 aaaataagaa aaatcaaaaa atcaaaaaac aaaacaggtt atgctattta tgcagaagca
     7441 gaaataaaag gaggtaaagc acggtttgta atagtttcat taatggctaa tggtaaaata
     7501 aaaaataaag atattaaaaa aataaaaaga aataaacatt actattttta gcttttttca
     7561 tatcgtcctc ataggggtag ttctggtttt ttatattgtc catatctgat agattatcaa
     7621 gaagttttta ttgatgggaa aaatataatt atagataaaa ctcattattt acgagcagtt
     7681 tatactaccc caaacctaaa aggtttatat tatttaaaac cttaagatta cttccttttc
     7741 ttcctttttt gcttagtatt aatatttctt ttttcgtgga aagcaccgcc tcc
//
LOCUS       DAOPNX010000109         7412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_11103_length_7412_cov_6.796248, whole genome shotgun sequence.
ACCESSION   DAOPNX010000109 DAOPNX010000000
VERSION     DAOPNX010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7412
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11103_length_7412_cov_6.796248"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..878
                     /locus_tag="PKN53_09840"
     CDS             108..878
                     /locus_tag="PKN53_09840"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOD89290.1"
                     /translation="MRTKEEIVNNWLPRYTGKSLDSFGEYILLTNFQLYVDLFAEYNN
                     VEVEGRDRNMTSATSENITIINFGMGSPNAATIIDLLSAISPKALLFLGKCGGLKAKN
                     KLGDLILPIAAIRSEGTSDDYLPVEVPALPAFQLQRAVSTSIRDHGRDYWTGTVFTTN
                     KRVWEYDERFKKYLRKTRAMAIDMETATIFIVGFANEIPSGALLLVSDQPMISTGIKT
                     DDSDRQVTENFVEDHIKIGTEALRLIARGGSSVKHLRF"
     gene            1009..4509
                     /locus_tag="PKN53_09845"
     CDS             1009..4509
                     /locus_tag="PKN53_09845"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HOD89291.1"
                     /translation="MKKVQLLFVFLLFSCFVFSQAYNGREADMRVKGADIVRFKDFSE
                     LPNFIHFGDAEHLDKAKAIDLTNSFFEDNQLSLIEKNIQKNKSGDETHRFQQTYKNVP
                     IEFTAWNLQIKNGEVYALNGELFNNVNIVSNFSITPDEALNLALQYIGAEKYMWEDGS
                     EEKLLKTFLNDEDATYFPKAEKVLVPAQAKFENSNLHSAYKFDIYSKNPHQRKDVYVD
                     ATSGKILFSLSKLYESNAIGTAHTQYSGIQQINTTLDSESGQYILNDNTRGAGIRTLN
                     CQDTDDYEAAIEFYDSDNVWNNVNPQLDEYATDAHFATASTYDYFYNIHGRNSIDGNG
                     HALWSFVHFSLIDYGYGSNVNAFWNGYYMTYGDGNENITPLTTVDICAHEITHGLTSY
                     TANLIYQDESGALNEAFSDIFGTAVEFYAKPGNANYLIGSEIGYVMRSLADPNSTGKP
                     DTYQGSYWVFDESDNGGVHTNGIPLCYGFYLLSEGGSGTNDLGNFYDIASIGMAKAEK
                     IFFRLLTVYLTPSSNYYDAWFFAMQAAADLYGACSSEVQSVGEMFYAIGVADEPYVNT
                     AYANFTASYTQSCEAPFTVSFINQSYNGNSFLWNFGDGNTSAEINPTHTYNSEGYYNV
                     TLTVSGGGCGSAFKNKENFVQIDPSLPCVTFFPTYGNETITKCNGIIYDSGGPDGKYF
                     TDTHSSLTIYVAGSESIVLDILEFDIEEGSGIECNFDYIAFYDGPNTSSPLINETKYC
                     NTVGNPGIISSTGEYITIEFYSDSGLALEGFKIQYNCINSANPPTPIFSANNTYSCDG
                     YVEFVDNSINNPQTWEWNFGDGEISYEQSPTHKYLSSGIYTVQLKVGNENGSQTSIKT
                     DYIIIDLPEASQLVPLDNVCIDQAFVINQNAEGELKWYDTFESTIPVFIGNTWQHDAI
                     SEPVTYWVEEFYGSETYYVGSDNYNTDGGYFGHPSYIHYLIFDAYKPFVLKTVKVNAQ
                     GNGNRTIALRTSTQEIIAEKSVFCIDGTQIITLDFDVPVGNNLQLVGLGAPDLFRNSS
                     NVNYPYKIEDVVSIKASSASSEPYNYYYYFYNWKIQTYECTSARTEFEIIPHTCSNVE
                     DISLQNLSIFPNPSRGVFMFNNLDNLYFEFKISDVFGKVLLKGNSEQKEIDISYFANG
                     VYFVEINSESGNKFVKLVKQ"
     gene            complement(4603..5685)
                     /locus_tag="PKN53_09850"
     CDS             complement(4603..5685)
                     /locus_tag="PKN53_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOD89292.1"
                     /translation="MKTQFLSIIFCFIICFSANNIFAQSDSLNAESQNKHHTTQTLDK
                     DSIKIIREQLIDSIIDYAKTFVGLKYRWAGITPAGFDCSGFIYHIHRHFNLPMPRMSK
                     YSSMMGDRVHIDSLQPGDLVYFKTRSAINNTIGHVAMVIEKVPDSFIMIHSASHAGLV
                     IENFIEYSYYVSRYLYATRLPDEFYLRQWNDSLMEVYKHQNFDIYNDHKQAAVLAQQP
                     EGTTELIYTVKSGDVLGRIASWYKVSVNELMAWNGLGSTRLSIGQKLKVYVKDALVST
                     YKGVDQMSYDDKQRLAGVSTTATSTNTEVKKDPAYEYYTVQAGDNPYSISKKFPGTTA
                     DGIMQLNRITDPSKLRIGQNLKIRKK"
     gene            5801..6685
                     /locus_tag="PKN53_09855"
     CDS             5801..6685
                     /locus_tag="PKN53_09855"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOD89293.1"
                     /translation="MKFLEFIAFRILVAIFWLLPFWVIYLLSDLLFFVLYYVVKYRRA
                     VAYDNLSKCFPDKSDQEINKMNKEFFHHLMDITLESVKGMSISQKKIEKRYILKNTEI
                     INSYYYENKSVMCLTSHYGNWEYGILGTDLGIKHQAVALYLPLSNKFSERYGLKRRRR
                     FGMQMVEVSKTREIFANIPPKPVAIIMAADQSPANVEKAFKIKFLNRPTACLYGPEAY
                     AKKVGLPILYLKISKLKRGYYSLEFERLIESPKDLEAGEVTKIYFNRLEKDILEKPEY
                     WLWSHRRWKHNLDNYKIE"
     gene            6687..7322
                     /locus_tag="PKN53_09860"
     CDS             6687..7322
                     /locus_tag="PKN53_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD89294.1"
                     /translation="MAKIKTFVVNDFQTNAYLLYDETNEAVIIDGAANSNYEFERLNN
                     FITTNNLKLKYILNTHGHLDHICGNFHLKNTYNVPILMNFEDNYLVDNSLNFAYAYGF
                     VMEKPPQADLDIKDKSIITFGNTNIEVITCPGHTPGGVVFHVEKDNILIVGDSLFQQS
                     IGRTDLPGGNYETLIENIKNKIFTLNPQTIVFPGHGPETTIEDEIKYNPFF"
BASE COUNT         2583 a          999 c         1269 g         2561 t
ORIGIN      
        1 acttctgaaa caccttgaga ggttgttggt tcaacacttg ttttgagaca acctcttttt
       61 tttgttatta tattttataa tttattattt ttgtaaaaaa atagtttatg agaacaaaag
      121 aagaaatagt taataattgg ttgccacgtt atacaggaaa atctcttgat tcatttggag
      181 aatatatttt gttaacaaat ttccaacttt acgtagattt gttcgccgaa tataataatg
      241 tggaagttga aggacgtgat agaaatatga caagtgcaac aagtgaaaat attactatca
      301 taaattttgg aatgggtagc ccaaatgccg ctactataat tgatttatta agtgcaattt
      361 cgccaaaagc attacttttt ttaggtaaat gtggtggatt aaaagctaaa aataaattgg
      421 gagatttaat attaccaatt gcagcaatta ggagcgaagg aacatctgac gactatttac
      481 cagtagaagt accagcttta cctgctttcc aattacagag agcagtttca acttctattc
      541 gtgatcacgg tagagattat tggactggaa ctgtatttac aaccaataaa cgtgtttggg
      601 agtatgacga aagatttaaa aagtatttac gaaaaacacg tgcaatggct attgatatgg
      661 aaacagcaac tatatttatt gttggatttg caaacgaaat cccttcaggt gctttgcttt
      721 tagtaagcga ccaaccaatg atttcaacag gtataaaaac tgacgatagc gatagacaag
      781 ttactgaaaa ctttgttgaa gaccatatta aaataggaac agaagcatta cgattaattg
      841 caagaggagg ttcttctgtg aaacacttgc gtttttagtg gttttggcgg aatttttgta
      901 aattttacaa aaattatgac aaaacacatt catattgttt tttttaacat aaaattctgt
      961 cttaatatct gttttttttc ataactttac aaaataaaat tattgaatat gaaaaaagta
     1021 cagctattat ttgtttttct gttattttct tgttttgttt tttctcaagc atataacggt
     1081 agagaagcag acatgagagt gaaaggtgct gatatagtgc gttttaaaga tttttcggaa
     1141 ttacctaact ttatacattt tggcgatgct gaacatttag ataaggcaaa agctattgat
     1201 ttaacaaatt ctttctttga ggataatcag ttaagcttaa tcgaaaaaaa tattcaaaaa
     1261 aataaaagtg gagacgaaac tcatagattt caacaaactt ataaaaatgt cccaatagag
     1321 tttacagctt ggaatttaca aattaaaaat ggagaagttt atgctttaaa tggagaactg
     1381 tttaataatg taaatattgt ttcaaatttc tctattactc cagacgaagc tttgaatttg
     1441 gctttacaat atataggtgc agaaaaatat atgtgggaag atggtagcga agaaaaattg
     1501 ttaaaaacat ttttaaatga tgaagatgca acatactttc caaaagcaga aaaagtatta
     1561 gtacctgctc aagcaaaatt tgaaaattca aacttacatt ctgcatataa atttgatatt
     1621 tattcaaaaa atccacatca aagaaaagat gtttatgttg atgctacaag tggcaaaata
     1681 cttttttctt tatcaaaact ttatgaaagt aatgcgattg gaacggctca tactcaatat
     1741 agcggaatac aacaaatcaa tactacactt gactccgaaa gcggacaata tattttaaac
     1801 gacaatacgc gtggagccgg aattagaact ttaaattgtc aagatactga tgattacgaa
     1861 gctgccattg aattttatga ttctgataat gtttggaata atgtaaatcc tcaacttgat
     1921 gaatatgcta cagatgctca ttttgcgact gcctcaactt atgactattt ttataatatt
     1981 catggacgta atagtattga tggtaacgga catgcacttt ggtcttttgt acattttagt
     2041 ttgatcgact atggctatgg aagtaatgta aacgcttttt ggaatggata ttatatgact
     2101 tatggcgatg gaaacgaaaa tataacacca ctcacaactg ttgatatttg cgctcatgaa
     2161 attactcatg gcttaacttc atatacggca aatttaatct atcaagatga atcaggggct
     2221 ttgaacgaag catttagtga tatttttggt actgctgttg agttttatgc aaagcctggt
     2281 aatgcaaatt atttaatagg ttctgaaata ggttatgtta tgcgttcact agccgatcct
     2341 aattcaactg gtaaacccga tacttaccaa ggcagttatt gggtttttga tgaaagcgat
     2401 aatggaggtg tacatacaaa tggaatccct ttatgttatg gtttttattt attaagcgaa
     2461 ggtggtagcg gcacaaacga tttgggaaac ttttacgata ttgcttcaat tggaatggca
     2521 aaagcagaaa aaatattttt tcgtttgtta acggtttatt taacacctag ctcaaattat
     2581 tatgatgctt ggttttttgc aatgcaagca gcagccgatt tgtatggagc ttgctcaagc
     2641 gaagtgcaaa gtgtgggtga aatgttttat gcaattggag tagctgatga accctatgtt
     2701 aatacagctt atgcgaattt tacagctagt tatacgcaaa gttgtgaagc accttttact
     2761 gtgagtttta taaatcaatc ctataatggg aacagttttc tttggaattt tggagacgga
     2821 aatacttcag ctgaaataaa tcccacacat acttataata gtgaaggtta ttataatgtt
     2881 acattaactg tttctggagg aggttgcggt tcagcattta aaaataaaga aaattttgta
     2941 caaatagacc caagtttgcc ttgtgtaaca ttttttccaa cttatggtaa tgaaacaatt
     3001 actaagtgta atggaataat ttatgattct ggaggacctg atggaaaata ttttacagat
     3061 acacattcaa gtttaactat ttatgtagct ggttcagaaa gcattgttct agatatttta
     3121 gaatttgata ttgaagaagg tagtggaatt gaatgtaatt ttgactacat agcattttat
     3181 gatggaccaa acacttcttc tccattaata aatgaaacta agtattgcaa taccgtaggg
     3241 aatccaggaa taatttcttc gacaggagaa tatattacaa tagaatttta ttctgattca
     3301 ggtttagcac ttgaaggttt taaaattcaa tataattgta taaattctgc caatccgcca
     3361 actccgattt tttcagctaa taatacttac tcatgtgatg gatatgttga atttgtagat
     3421 aattcaatta ataatccaca aacatgggaa tggaattttg gtgatggaga aatttcctat
     3481 gaacaaagtc ctactcataa atatttaagt tctggcattt atacagtaca attaaaagtt
     3541 ggaaatgaaa atggctctca aacttcaata aaaactgatt atataataat tgatttacca
     3601 gaagcatcgc aattagttcc gcttgacaat gtttgtattg accaagcttt tgtaataaat
     3661 caaaatgccg aaggtgaatt gaaatggtat gatacttttg aaagcacaat tcctgttttt
     3721 attggaaata cttggcagca tgatgcaatt tcagaacctg taacctattg ggtagaagaa
     3781 ttttatggaa gtgaaaccta ttatgtaggt tcagataatt ataatactga tggaggatat
     3841 tttggtcatc cttcttatat tcactatttg atttttgatg cttataaacc atttgtgcta
     3901 aaaacagtaa aagtaaacgc acaaggaaat ggaaatcgaa caattgctct aagaacatcg
     3961 actcaagaaa ttattgctga aaaatctgtc ttttgtattg atggaaccca aattattact
     4021 ttagattttg atgtgcctgt gggaaataat ttacaattag ttggtttagg cgctccagat
     4081 ttatttagaa atagtagtaa tgtaaattat ccttataaaa ttgaagatgt ggtaagtata
     4141 aaagcaagct ctgcaagctc agaaccatat aattattatt actactttta taattggaaa
     4201 attcaaactt atgaatgcac ttcagcaaga acggaatttg aaattattcc tcacacatgt
     4261 tcaaatgtag aagatattag tttgcaaaat ctttcaattt ttccaaatcc aagtagagga
     4321 gtgtttatgt tcaataattt agataattta tattttgaat ttaaaataag cgatgttttt
     4381 ggtaaggttt tattgaaagg aaattctgaa caaaaagaaa tagatatttc atattttgca
     4441 aatggagtgt attttgttga aattaattca gaaagtggaa ataagtttgt gaaattggta
     4501 aaacaataaa aactcagtta aaagtttata aagtaaaaag ttcataaagg aatgccaagc
     4561 aaaaaacaac tttcaacttt caacttttaa cttttaactg acttactttt tcctaatttt
     4621 taaattttgc ccaattcgta attttgaggg gtcggtaatt ctgtttagtt gcattatgcc
     4681 atcggcggta gtgccaggga attttttaga aatagaatat ggattatctc cagcttgtac
     4741 tgtgtaatat tcgtaagcag ggtctttttt cacttctgtg tttgttgagg tggcggttgt
     4801 gctaactcct gctaatcgtt gtttgtcatc ataactcatt tggtcaacac ctttgtaagt
     4861 tgaaacaaga gcatctttta cataaacttt tagtttttgt cctatagaaa gtctggtaga
     4921 gccaagtcca ttccatgcca tgagttcatt aaccgaaact ttataccacg aagcaattct
     4981 gccaagcaca tctcctgatt ttactgtgta aataagctct gttgttcctt ctggttgttg
     5041 ggctaatacc gcagcttgtt tgtggtcgtt ataaatatcg aaattttggt gtttatatac
     5101 ttccattaag ctgtcgttcc attgtcgtaa ataaaactcg tctggtaatc tggtggcgta
     5161 taaataacga ctaacatagt atgaatattc aataaaattt tcaattacca aacctgcatg
     5221 acttgcagag tgaatcataa taaaagaatc aggaactttt tcaattacca ttgcaacatg
     5281 acctattgta ttatttattg cagagcgagt tttaaaataa accaaatcac cgggttgcag
     5341 gctgtctata tgaactctat cgcccatcat gcttgaatat ttcgacattc gtggcattgg
     5401 taaattaaaa tgtctgtgaa tatgatatat aaaaccgcta caatcaaaac cagcaggagt
     5461 aattccagcc caacgatatt ttaaacctac aaaagtcttt gcatagtcaa taattgaatc
     5521 tataagttgc tctctaatta ttttaataga gtctttatct aatgtttgtg tagtatgatg
     5581 cttattttga ctctctgcat ttaaactatc gctttgtgca aaaatattgt tagctgaaaa
     5641 acaaataata aaacaaaata ttatgctaag aaactgtgtt ttcattttta aaatttatgt
     5701 atattaaatt aatataattc aaattaagtt tcacaactgc aaatatagaa aaattttaaa
     5761 aattagtaat tttatttaat tttgctccgg ttaaatatta atgaaatttt tagaatttat
     5821 agcatttaga attttggtag ccatattttg gcttttacca ttttgggtaa tttatctttt
     5881 atccgattta ctattttttg ttttatatta tgttgtaaaa tatcgtagag ctgtagctta
     5941 tgataatttg agcaaatgtt tcccagacaa gtcagaccaa gaaataaata agatgaataa
     6001 ggaatttttt catcatttaa tggatattac tttggagtcg gtaaagggaa tgagcattag
     6061 tcaaaaaaag atagaaaaaa gatatatact taaaaataca gaaattatta attcgtatta
     6121 ttatgaaaat aaatctgtaa tgtgtcttac cagccattat ggaaactggg aatatggaat
     6181 tttaggtaca gatttgggaa taaagcatca ggctgtggcg ttatatttac ctttaagtaa
     6241 taaatttagc gaaagatatg gtttaaaacg ccgtcgtaga tttggaatgc aaatggtcga
     6301 agttagtaaa acaagagaaa tttttgcaaa cattccacca aaacctgttg ctataattat
     6361 ggctgccgac caaagtcctg caaatgttga aaaagctttt aaaattaaat ttttaaatcg
     6421 tcctactgct tgtttatatg gacccgaagc ttatgcgaaa aaggtaggat taccaatttt
     6481 gtatttaaaa atttcaaaac ttaaaagagg atattattca ttagaatttg aaagattgat
     6541 tgaatcgcca aaagatttag aagcagggga ggtaacaaaa atttacttta atagattaga
     6601 aaaagatata cttgaaaagc ctgaatattg gctttggtcg cacaggcgtt ggaaacataa
     6661 tttagataac tataaaattg aataatatgg caaaaattaa aacttttgtt gttaatgatt
     6721 ttcaaactaa tgcttatttg ctttatgacg aaacaaatga agctgtaata attgatggag
     6781 ctgcaaattc taattatgaa tttgaaagat tgaataattt tataacaaca aataatttaa
     6841 aactcaaata tattcttaat actcatggac atttagacca tatttgtgga aattttcatt
     6901 taaaaaatac atataatgtc ccaattttaa tgaattttga agataattac ttggttgata
     6961 attctttaaa ctttgcttat gcctatggtt ttgttatgga aaaaccaccg caagcagatt
     7021 tagatataaa agacaagtct atcattacat ttggtaatac aaatattgaa gtaattactt
     7081 gtcctggaca tactccaggt ggtgtggttt ttcatgtcga gaaagataat attttaattg
     7141 taggcgattc tttatttcag caaagcattg gtagaactga tttgcccggc ggaaattacg
     7201 agacgctgat agaaaatatt aaaaacaaaa tttttactct caatccacaa actattgttt
     7261 tccccggtca tggacctgaa actactatcg aagatgaaat taagtataat cctttttttt
     7321 gaattaagaa tttataattg agagttaaga atttctcaat tataattatt caaaaaaatg
     7381 tagatatatt gttgattttt cttggacgtt tc
//
LOCUS       DAOPNX010000110         6965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_11978_length_6965_cov_6.864834, whole genome shotgun sequence.
ACCESSION   DAOPNX010000110 DAOPNX010000000
VERSION     DAOPNX010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11978_length_6965_cov_6.864834"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1357)
                     /gene="glyA"
                     /locus_tag="PKN53_09865"
     CDS             complement(77..1357)
                     /gene="glyA"
                     /locus_tag="PKN53_09865"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOD89295.1"
                     /translation="MKRDKKVFEIIEKERARQLHGIELIASENFVSEQVLEAMGSVLT
                     NKYAEGYPGKRYYGGCQFVDQTEQLAIDRLKELFDAEYANVQPHSGAQANAAVFLACL
                     KPGDTFMGFDLAHGGHLSHGSPVNLSGILYNAISYKVKEETGTVDYDEMEKLALEQKP
                     KMIIGGASAYSREWDYKRMREIADKIGAIFMVDMAHPAGLIAAGLLNNPAKYAHIVTS
                     TTHKTLRGPRGGIIIIGKDFENPWGIKSPKGEIRMMSELINSAVFPGTQGGPLEHVIA
                     AKAVAFGEALSPEFKEYQKQVKKNAATMAKAFIDAGYKIISGGTDNHSMLIDLRTKYP
                     DVTGRQVENALVQADITINKNMVPFDTRSPFQTSGIRIGTPAVTTRGLKENHMPIIVD
                     LIDRVIADYTNEKVIEKVRAEVNEMMKPFPLFSY"
     gene            1479..2585
                     /locus_tag="PKN53_09870"
     CDS             1479..2585
                     /locus_tag="PKN53_09870"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOD89296.1"
                     /translation="MYYLNFETLEKISVQALVAAGVPENDAKIVADVLLEADKLGIDS
                     HGINRLKPIYLDRIKDGILNPVTKIDTIRETFTTAVLDANNGMGHVVSKKAMEICIEK
                     AKTYGMGMVAVRNSSHYGIAGYYGLMAAKEGMIGITGTNARPSIAPTFGVENMLGTNP
                     LTFVMPSDEEFPFFLDCATSVSQRGKFEYYAKIGKDLPKGWAIDKNGESKTDSKQVLQ
                     DLVDGNAALTPLGGIGEETAGYKGYGYATVVEILSAALQGGGFMKALLGFENGKKVPY
                     RLGHFFIAINIEAFIELDEFKKATGNILRDLRNSEKMPGKDRIYTAGEKEYEAFLERS
                     KTGVPINQSLWEEIKNIILEYKLKDFYKIFKIND"
     gene            2610..3530
                     /locus_tag="PKN53_09875"
     CDS             2610..3530
                     /locus_tag="PKN53_09875"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOD89297.1"
                     /translation="MKVIENWFRASDGKDIFYNEFLPDNIDDVKFVVQIFHGMAEHSE
                     RYTHFAEFLTSKGAAVYASDHRGHGRNCLRPGEYGVWAKKGAWFTIVDDLKVLNDITA
                     KNFPDKPNFILGHSMGSFLARSFITKYSRGLNGVILSGTGTNSNLVLNTAKAIAWKQC
                     NFRSPAKPAKLLDKLSFSSFNKSYDKPYQWLTRDQKIIDEYIDDPYCGGVFSSSFYYN
                     FFSGLLHVNQLNKAKKIAKDLPLFFISGDKDPVGNYGKGVEQAVEFYKKAKLTNIDVK
                     LYHEARHEILNEINKDEVYNDILNWITKHL"
     gene            3534..5015
                     /gene="glpK"
                     /locus_tag="PKN53_09880"
     CDS             3534..5015
                     /gene="glpK"
                     /locus_tag="PKN53_09880"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOD89298.1"
                     /translation="MSKKYILALDQGTTSSRAILFDDNFDIVGIEQSETKQIFPKPGW
                     VEQNAIEIYETQVYVARKLLAKLSINNNDIIGIGITNQRETTIVWDKNTGVPVYNAIV
                     WQDKRTAECCEKLRNSEFGNYVNDKTGLVVDAYFSASKIQWILDNVDNARQKAENGEL
                     LFGTVDTWLVWNLSGKKLHVTDYSNASRTMLFDIHNLDWDEKILEYFNIPKQILPKVL
                     NSSKIYGKTAKQIFGNNQIDIAAILGDQQASMFGHNCFNLGDVKNTYGTGCFMLMNIG
                     EKFAKSKSGLVTTIAWGIENKITYALEGSVFVAGAVIQWLRDKLKIIETAQETEEIAL
                     SVKSSEGVYFVPAFAGLGAPYWDMNAKAAIHGITGATNYKHIVRAAVESMALQTKDVL
                     EAMQKDANAKINVLNVDGGAAANNFLLQFQADILNIEVLKPKILESTALGVAFMVAIS
                     TKFKTLDEILKIRKIEKKFTPKMKETERNFVYSQWLKAVDRVR"
     gene            5025..6128
                     /gene="mqnE"
                     /locus_tag="PKN53_09885"
     CDS             5025..6128
                     /gene="mqnE"
                     /locus_tag="PKN53_09885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HOD89299.1"
                     /translation="MQKFLSKYSSEHQEIAKSVFENKRLSFDQALFLYENADPIFCGL
                     LADYKRTKLYQNKIFFNKNIHIEITNICVNKCKFCSFFREKGQDGSWDLEAEDVLEIV
                     KEKVSTRITELHFTGGLHSKKTTDFYSNLFKKIKKTYPHLHIKAFTSVEIEHFSKVDG
                     INLFELLKLLKESGLNSLAGGGAEILNDDIRKKICPEKTNSETWLQIHKYAHQLGLKT
                     NCTMLYGHIETNKDRVKHLELLRNLQDETNGFNSFIPLKYKIQGNKLALKQEASLIDE
                     LKNYSISRLFLDNIPHLKAYWPMCGKETALLAMSFGADDFDGTISNTTKIYSMAGAEQ
                     KPELGCDEIKELAENAGFIAVERDSEYRELIFF"
     gene            complement(6170..>6965)
                     /locus_tag="PKN53_09890"
     CDS             complement(6170..>6965)
                     /locus_tag="PKN53_09890"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HOD89300.1"
                     /translation="MKKFPISAFLSILLIVILVASCTIQKRRYMPGYHIDRIGSRPVA
                     ETKSENKNFNKTADFVEVQEQLNATNATETNKTVEEPLLYASDDDLNIIPQESENSTL
                     VSKAKNDSKKLVTTSDIECDIIILRNGDEIAAKVLEVGQNEIKYLMCDNIYGPTFTVN
                     KSEIFKINFSNGTSTVFTQPNSNRSNDNDSIIPKSNNPKDKSLVVAIVLWFFLGLLGI
                     HRFYLGHIGMGILYLLTGALCGIGWIIDIILLVTGGLQPKGGKYID"
BASE COUNT         2443 a         1019 c         1192 g         2311 t
ORIGIN      
        1 ctgaaacacc ttaaaaattc attgtttcaa aatttgtttt gagacagcct cttttattta
       61 tacagttatg tgttttttaa taagaaaaca aaggaaatgg tttcatcatt tcgttcactt
      121 ctgctcttac tttttctatt actttttcgt ttgtatagtc ggcaataact ctgtcgatta
      181 agtcaactat aattggcatg tgattttctt ttaacccgcg tgttgtaaca gctggcgttc
      241 caattctaat acctgaggtt tggaaaggag aacgagtgtc gaaaggaacc atgtttttgt
      301 taattgtaat atctgcttgc accaaagcat tttcaacttg tcttcctgta acatcaggat
      361 atttagttct taaatcaatt aacatagagt ggttatctgt tccgccactt ataattttat
      421 agccagcgtc tataaatgct tttgccattg tagctgcatt tttcttaact tgcttttggt
      481 attctttaaa ttctggactt agagcttctc caaaagctac tgctttagct gcaataacgt
      541 gttcgagtgg accaccttgc gtgcctggga aaactgcgga attgataagt tctgacatca
      601 ttcttatttc accttttggt gattttattc cccaaggatt ttcaaaatct ttacctataa
      661 taataatacc accacgtgga cctcttaaag ttttgtgagt tgtagaagtt acaatatgtg
      721 catactttgc tggattgttc aacaatccag cagcaattaa acctgctggg tgagccatgt
      781 caaccataaa aatagcacct attttatctg caatttcgcg catgcgttta taatcccact
      841 cacggctata agcagatgct ccaccaataa tcatttttgg cttttgttct aaagcaagtt
      901 tttccatttc gtcgtaatca acagtaccag tttcttcttt cactttgtac gaaatagcat
      961 tatacaaaat tccacttaaa tttactggag aaccatgtga taaatgacca ccatgagcca
     1021 aatcaaagcc cataaaagtg tcgccaggtt ttaaacatgc taaaaataca gcagcattag
     1081 cttgagcacc agagtgtggc tgaacatttg catattctgc atcaaacaat tcttttaatc
     1141 tgtcaattgc taactgttcg gtttggtcaa caaattgaca accaccataa tatctcttgc
     1201 caggataacc ttcagcatat ttgtttgtca atactgaacc catagcttct aaaacttgtt
     1261 cacttacaaa attttctgaa gcgattaatt ctatgccatg caattgtctg gctctttctt
     1321 tttcaataat ttcaaatact tttttgtcgc gtttcataat tattattttt ttttgtaaaa
     1381 ataagaaatt taatttcaat aaaaaaatag attaatctgt tttataaaat gagtttaaaa
     1441 ttataatttt attttttata tttgtgaaaa attacaagat gtattatttg aattttgaaa
     1501 ctttagaaaa aatttctgtt caagcattag tcgcagcagg agtgcctgaa aatgacgcta
     1561 aaattgttgc tgatgttttg ttagaagctg ataaattggg aatagattct catggaatta
     1621 ataggttgaa accaatttat ttagatagaa ttaaagatgg aattttaaac cctgtaacaa
     1681 aaattgatac tattcgcgaa acttttacaa ccgctgtttt agatgcaaat aacggaatgg
     1741 gacatgtggt ttctaaaaaa gcaatggaaa tttgcataga aaaagctaaa acctatggca
     1801 tgggaatggt agcagtaaga aatagctctc attacggaat tgccggatat tatggtctta
     1861 tggctgcaaa agagggtatg atagggataa caggaaccaa tgcacgacca tctattgcac
     1921 ctacttttgg tgtggaaaat atgttgggaa caaatccact tacttttgtg atgccaagcg
     1981 acgaagaatt tcctttcttt ttagattgtg caacctcggt aagccaaaga ggtaagtttg
     2041 agtattatgc aaaaattgga aaagatttac caaaaggctg ggctattgac aaaaatggag
     2101 aatcaaaaac agattctaag caagttttac aagatttggt tgatggaaat gcagcactta
     2161 cacctcttgg aggaatagga gaagaaacag caggatataa aggttatggc tatgctactg
     2221 ttgtagaaat tttatctgct gctttgcaag gaggaggctt tatgaaagca cttcttggat
     2281 ttgaaaatgg taaaaaagtg ccatacagat taggacattt ctttatcgca ataaatatcg
     2341 aagcttttat tgaactagat gaatttaaaa aagcaacagg aaatatttta agggatttaa
     2401 gaaattctga aaaaatgcca gggaaagata gaatttatac tgcaggcgaa aaagagtatg
     2461 aagctttttt agaaaggtcg aaaacaggag tgccgataaa tcaatctttg tgggaagaaa
     2521 ttaaaaatat aattttagaa tataaactta aagattttta taaaattttt aaaattaatg
     2581 attaagtttg tgattattta gtgtgaaaaa tgaaagtaat tgaaaattgg ttcagagcat
     2641 ctgatggaaa agatattttt tataatgagt ttttgcctga taatatagac gatgtaaaat
     2701 ttgttgttca aatttttcat ggaatggcag aacactcgga aagatatact cattttgcag
     2761 aattcttaac tagtaaagga gcagctgttt atgcatctga ccatagagga catggaagaa
     2821 attgtttaag acctggagaa tatggagtat gggcaaaaaa aggagcttgg tttacaatag
     2881 ttgacgattt aaaagttctt aacgatatta cagcaaaaaa ctttccagat aaaccaaact
     2941 ttatattagg gcatagcatg ggttcttttt tagctcgttc ttttataaca aaatatagca
     3001 gaggattaaa cggggtaata ctttcgggaa caggaacaaa ctcaaattta gttttaaata
     3061 ctgcaaaagc tattgcttgg aagcaatgta attttagaag cccagcaaaa ccagcaaaac
     3121 tgttagataa attgtcgttt tcaagcttta ataaatctta cgacaaacca tatcaatggc
     3181 taactcgaga tcaaaaaatt attgacgaat acattgacga tccttattgt ggaggagttt
     3241 tttcttcttc tttttattat aattttttct ctggcttatt gcatgtaaac caattgaata
     3301 aggcaaaaaa aattgcgaaa gatttacctt tattttttat ttcgggagat aaagacccag
     3361 ttggaaatta tggaaaagga gtggagcaag ctgtagaatt ttataaaaaa gcaaaactta
     3421 caaatattga cgtaaaacta tatcacgaag ctcgacacga aatccttaac gaaataaata
     3481 aagatgaggt ttataatgat attctaaatt ggataacaaa gcatttataa aaaatgtcaa
     3541 aaaaatatat tttagcactc gaccaaggta caacatcttc aagagcgatt ttgtttgatg
     3601 ataattttga tattgtagga attgaacaat ctgaaactaa acaaattttt cctaaacctg
     3661 gctgggtaga acaaaacgca atagaaattt atgaaactca agtttatgtt gccagaaaat
     3721 tacttgcaaa attatctata aacaataatg atattattgg aataggaata actaatcaac
     3781 gcgagacgac aattgtttgg gacaaaaaca caggcgtacc tgtttataat gcaatagtgt
     3841 ggcaagataa aagaactgct gaatgttgtg aaaaattacg taattctgag tttggaaact
     3901 atgttaatga taaaaccgga cttgtagtcg atgcttattt ttctgcttct aaaattcagt
     3961 ggatattaga taatgtggac aatgctcgac aaaaagcaga aaatggagaa cttctttttg
     4021 gaacagttga cacttggttg gtttggaatt taagcggtaa aaaacttcat gttacagatt
     4081 attcaaatgc ttctcgaact atgttgtttg atattcacaa cttggattgg gatgaaaaaa
     4141 ttcttgaata ttttaatatt ccaaaacaaa ttttaccaaa agttttaaat tcttctaaaa
     4201 tttatgggaa aacagcaaaa caaatttttg ggaataatca aattgatata gccgcaattc
     4261 ttggcgacca acaggcatca atgttcggac ataattgctt taaccttgga gatgtaaaaa
     4321 atacttatgg tacaggctgt tttatgttga tgaatattgg cgaaaaattt gcaaaaagta
     4381 aatctggact tgttacaaca atagcttggg gaattgaaaa caaaattact tatgccttag
     4441 aaggaagtgt gtttgttgca ggagctgtaa tccaatggct tagagataaa cttaaaatta
     4501 ttgaaaccgc tcaagaaacc gaagaaattg ctttgtctgt aaaatcaagt gaaggagttt
     4561 attttgtgcc tgcttttgct ggtttgggcg cgccatattg ggatatgaat gctaaggctg
     4621 ctattcacgg aattacagga gcaacaaact ataaacatat tgttagagca gctgtagaat
     4681 ccatggcttt gcaaactaaa gatgtgctag aagcaatgca aaaagacgca aatgcgaaaa
     4741 ttaatgtttt aaatgtagat ggaggtgctg ctgcaaataa ttttttatta caatttcaag
     4801 cagatatttt aaatattgaa gttttaaagc caaaaatcct tgagtcaaca gctcttggtg
     4861 tggcttttat ggttgcaatt tcaacaaaat ttaaaacttt agacgaaatc ttgaaaattc
     4921 gcaaaataga aaagaagttt acacccaaaa tgaaggaaac agaaagaaat tttgtttatt
     4981 ctcaatggct taaagcagtt gaccgagtaa gatgaaatta aataatgcaa aaattcttat
     5041 caaaatattc ttcagaacat caagaaattg caaaatctgt atttgaaaat aaacgactga
     5101 gttttgacca agctcttttt ctttatgaaa atgcagaccc aatattttgt ggcttacttg
     5161 ctgattataa aagaactaag ctatatcaaa ataaaatatt ttttaataaa aatattcata
     5221 tagaaattac aaatatttgt gtaaacaagt gtaaattttg ctcatttttc agagaaaaag
     5281 gtcaagatgg atcttgggat ttggaagctg aagatgtttt agaaattgta aaagaaaaag
     5341 tatcaacaag aattacagaa ttacatttta caggcggttt acattctaaa aaaacaacag
     5401 atttttattc aaatttattt aaaaaaataa aaaaaactta tcctcattta catattaaag
     5461 catttacatc tgttgagata gagcattttt caaaagttga tggaataaat ctttttgagc
     5521 ttttaaaact gttaaaagaa tctggtttaa actcgcttgc aggtggaggt gcagaaattt
     5581 taaatgatga tattcgtaaa aaaatatgtc ccgaaaaaac aaattctgaa acttggttgc
     5641 aaattcataa atacgctcac caacttggct taaaaaccaa ttgcacaatg ctttacggac
     5701 atattgaaac gaacaaagac agagtaaaac atcttgaact tttacgcaat ttgcaggacg
     5761 aaacaaatgg atttaatagt tttattcctt taaagtacaa aattcaagga aataaacttg
     5821 ctttaaagca agaagcctct ttgattgatg aattgaaaaa ttattctatt tctcgtttgt
     5881 ttttagataa tataccacat ttaaaagcat attggcccat gtgtggcaag gaaacagctc
     5941 ttttggcaat gtcgtttgga gcagatgatt ttgatggcac aatttcaaat actacaaaaa
     6001 tttattctat ggctggtgcc gaacaaaaac ctgaactcgg ttgtgatgaa attaaagagc
     6061 tcgccgaaaa tgcaggattt attgctgtag aaagagattc tgaatatagg gagttgattt
     6121 ttttctaaaa agttgagttt tagttcttta tttcaaaaat tataggttac taatcaatat
     6181 attttcctcc cttgggctgc aaccctcctg ttacaagcaa tattatatca ataatccaac
     6241 caattccaca caatgcccct gttagtaaat ataatattcc catgcctata tgtcctaaat
     6301 aaaatctatg tattcctaaa agtcctaaga aaaaccataa aactatagca acaactaaac
     6361 ttttatcttt tggattgtta ctttttggta ttatagaatc attgtcatta ctgcgatttg
     6421 agttaggttg tgtgaatact gttgatgtgc cattagaaaa attaattttg aatatttcac
     6481 ttttatttac tgtaaaagtt ggtccataaa tattatcaca catcagatat ttaatttcat
     6541 tttgtccaac ctctaaaact tttgctgcaa tttcatctcc gtttcttaaa ataattatat
     6601 cacattcaat atctgaagtg gtaactaact tttttgaatc attttttgct tttgaaacta
     6661 gagttgaatt ttctgattct tgtggtatta tgtttaagtc atcatctgaa gcatataaaa
     6721 gaggctcttc gacagttttg tttgtttcag tagcattagt agcattaagt tgctcttgta
     6781 cttcaacaaa atcagctgtt ttattaaaat ttttgttttc actttttgtt tctgctacag
     6841 gtctgctacc gatacgatca atgtgataac caggcatata acgtctcttt tgaattgtac
     6901 aagatgcaac aagaattaca atcaaaagta ttgataaaaa cgcactaata ggaaattttt
     6961 tcata
//
LOCUS       DAOPNX010000111         6852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_12226_length_6852_cov_5.752244, whole genome shotgun sequence.
ACCESSION   DAOPNX010000111 DAOPNX010000000
VERSION     DAOPNX010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6852
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12226_length_6852_cov_5.752244"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..382)
                     /locus_tag="PKN53_09895"
     CDS             complement(179..382)
                     /locus_tag="PKN53_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89301.1"
                     /translation="MRNKDSGFFFYVGDTFYLNEALLYNLITNGIFYDHKVNNLKLYY
                     SSEEAKKTLENNAKKWAKEQSEL"
     gene            complement(452..1312)
                     /locus_tag="PKN53_09900"
     CDS             complement(452..1312)
                     /locus_tag="PKN53_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89302.1"
                     /translation="MKKVITFIIFCTAIFNFSCSQNNYTQLDSIILFYNSNDSIGKYY
                     KYVYKAEDYIMMENFEKAAETYEKAFTYIDIPFLQDLGNAKECEMNSRKNEECIKKYI
                     YFLIRKNGTKDGYLKNEELQKLETWSDIKAMIDTLSCLNDTAVVNELERIIFNDQDYR
                     NQCHKKYNGNTYNEFTRDSIAKIDSINYYKIYRMFENIDMSEEIFGYRGWGIIDVILM
                     HNRQRKEIYELLIKSVFDGKLNARDVFSSLDDSNYKFENKYLFGGRSFGFNVNDDCYM
                     YIKPNFKQNL"
     gene            complement(1479..1940)
                     /locus_tag="PKN53_09905"
     CDS             complement(1479..1940)
                     /locus_tag="PKN53_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOD89303.1"
                     /translation="MQYAFLYGTSAGGYTVAHLANEYPQFFKGIILNVPEVTILQGLQ
                     KKYDPFYLLNADLHGNIEDEKVYDFIKKSDPYINTRIGQYPNMLIFSAYNDENVPVEN
                     VAKYVARLRECNIGNNLVLFRTHMNEDHDGGQYGQLQRTAIIYFFLLNLAY"
     gene            complement(1892..4840)
                     /locus_tag="PKN53_09910"
     CDS             complement(1892..4840)
                     /locus_tag="PKN53_09910"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOD89304.1"
                     /translation="MCNRVITFVLLFLLNAITVVSQNSLTGTIQDFDTGERLQYANIS
                     LLNSPVGTMTNENGEFVLYLNKTNLKDTLLISFLGYSNLKIPVDSAFFKQNVFRLKKS
                     IEFINEVTVKPIDPFELLQNALLKKSYLYNVNAYNSEGFYREIMLENNEPVKLAEAAC
                     LFYISPYASKFNRNDAIWNYLDFSAYNSNYRYDQALNYSTNATYPDDAVQIISARSSD
                     DFTGSISKFCIAGGPLNITATDFFRNETFYFSSSGLKTLKNKKKFTYHIDFSIYDGKN
                     VYDLNFVSKQNSDWLRVIVERESGAIISYSGKYTLNNSSQMERTGQIKGQKKTVNDIV
                     TEKELNFRVDFKNIKGLWYLNHIKSINTYEYKYLLEDITVQMAYDRELVFNNYNTEGV
                     KKIADNLAYKNIISNSLYDYPTSYDKDFWNNYNMLYPTELQTEILNKIEATKSLEDQY
                     QSKFISFDEMEKPFALNVIDTSYIHNDTIVDTYAWTKNNSELLDYIEKENKYVLSYLY
                     ENNSLIKGIYNELKSYELKHKYEFSFDTINSYYYFFSLEGNFCRSSSPDTSANEVVIN
                     LVEKTIQNSQYALYDYSFNEDAGVLTYIESPDGGRNLNLICINIENLGKICELKNVMQ
                     LEWIDNNRFLYTSLDDNDFFPTQVYLHDIKSNVDSLIYKEVDERKKLSLKRSASNKFI
                     FIESFSFSDNYSTLAINTENENCSIVAFKNIPDSVYAKFDHYNNKFYIRTNYSKPNFS
                     IMVLPENEHDIKKAENIFSFLSDTTITELNFNAGKLLLSFQHGITTDFGIVDEATLSF
                     KHLKWKENNYTVSFCKNISETKTELSFESYLEPPQKWIYDIPNDIISQTKHTEIRGYN
                     KNKYEVKIANAKHSDGITIPIVMVGMKKGKKTRPMLMEVYAAGMGKSLLPGFRSDIFP
                     LLDRGFIYAYPLVRGSGELGYEWYKKGAKQNKTKTFEDVAVCAKWVQENKLTTYNTLF
                     YTAPVLEDIQ"
     gene            complement(4932..5525)
                     /locus_tag="PKN53_09915"
     CDS             complement(4932..5525)
                     /locus_tag="PKN53_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89305.1"
                     /translation="MKNYIQKSLEMGKSYKRVKNKYSNFYKNETFWKEMKAVSIKACE
                     RIEMESDSIYLYLQEMFLNDQKARKSVFNKKAKMKVADSVNTIKLNRIVSENYYPGFL
                     TIGLHNAYNEIIITDVLMLHLSVDNFEKIIVNAMTRGEVTPYMACYIFIRQYKANYKT
                     TQEPEAIPDLIKKCLKYYKGEKYEQLQKKGFDFHAII"
     gene            complement(5883..>6852)
                     /locus_tag="PKN53_09920"
     CDS             complement(5883..>6852)
                     /locus_tag="PKN53_09920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOD89306.1"
                     /translation="GDIVDFEVIISGGGIDPNQVWVGDSLWNLPVSIAINQDTTIIFE
                     VFDACKFNHITKPISISTYPLPLMNIAAEPTNGCSPLEVQFYENSPDVGQRYVWNFDD
                     GDFENLSFDKYPKHTFYNARTFNVGLEITSTDGCKRDSTIGIVVFPNPEVEFRAHTTN
                     VTMASPLIYFTNYTHGGFWHKWSFGDGNFSDDTNPSHTYSMPGTFHVTLVSESLYGCV
                     DSAGVDIYVSNELAVYAPTAFTPNYDDLNETFKVIVDGIDEKTYSLIVYNRWGEIVHK
                     SEGYEKEWNGRYGDMECPPDVYVWVVKFVDNYGNEHTYTGNVTLIR"
BASE COUNT         2320 a         1141 c          836 g         2555 t
ORIGIN      
        1 ttgccctaat gcaaatttat tttgtaaaca gaaacaaaaa atcaaaataa taaaaacaat
       61 agatttattc ataaaatatt gcttaaattc tgtttttttg aacttttcgc gatttaaact
      121 ttttaaattc ataatatgta attttttaaa ttaataattc aactgtgaac aatctttatt
      181 acaactcact ttgctctttt gcccactttt tagcattatt ctcaagagtt tttttagcct
      241 cttctgatga ataataaagt tttaaattgt taactttatg atcataaaat attccattag
      301 taattaaatt gtataaaaga gcttcgttca aataaaaggt atctcccaca taaaagaaaa
      361 agccactgtc cttatttctc atttgccaaa ttctcttttt atgaaaatct gatatattct
      421 caagataaat tttttgtcga tatttattta tttataaatt ctgtttaaaa ttgggtttta
      481 tatacatata acaatcatcg ttaacattaa atccaaatga cctaccgccg aataaatatt
      541 tattttcaaa tttataattg gaatcatcta agcttgagaa aacatctctt gcatttaatt
      601 taccatcaaa aaccgatttt atcaaaagtt catatatctc ttttcgttgc ctattatgca
      661 taagtattac atcaataatt ccccaccctc tataaccaaa aatctcctca gacatatcaa
      721 tattttcaaa cattctgtag attttatagt aatttatgga gtcgatttta gctatagaat
      781 ctctcgtaaa ctcattatat gtgttgccat tatacttttt atgacactgg ttacgataat
      841 cttgatcatt aaaaataatt ctttccaatt cgtttacaac agcagtatca tttaaacaag
      901 acaaagtatc tatcatagct tttatatcag accatgtttc aagtttttgc aattcttcat
      961 ttttgaggta tccatctttt gttccatttt ttctaattaa aaagtaaata tattttttta
     1021 tgcattcctc attttttcga ctattcattt cgcattcttt tgcatttcca agatcctgca
     1081 aaaaaggaat atcaatataa gtaaaagctt tttcgtaagt ttcggcagct ttttcgaaat
     1141 tctccatcat tatataatct tcggctttat aaacatactt gtaatactta ccaatactat
     1201 cattagagtt ataaaacaag attattgaat ctaattgtgt ataattattc tgagaacaag
     1261 aaaaattaaa aattgctgtg caaaaaataa taaatgttat aacttttttc ataatgtatc
     1321 tgtttttaat aatccactta aaatcagcgt cttatatgtc attgtttttc tgtaacaaaa
     1381 gtaacaagtt ttataataat aacaaaaagg cattataaaa aattgtgcaa atttaaaaca
     1441 catctcaatt ctttgaatta agttggctta tttcatagct aataagcaag attcaacaaa
     1501 aagaaataaa ttattgctgt tctttgcaat tgtccatatt gacctccatc gtgatcttcg
     1561 ttcatatgag ttcgaaataa tactaaatta ttaccaatat tacattctct caatcgcgca
     1621 acatattttg caacattttc gacaggaaca ttctcatcat tatatgcact aaaaataagc
     1681 atgttaggat attgccctat tcgtgtatta atgtaggggt cgcttttttt aataaaatca
     1741 taaacttttt cgtcttcaat atttccatgt aaatcggcat ttagcaaata aaaagggtca
     1801 tatttctttt gtaaaccttg caaaatagta acttcgggaa catttaaaat tattccttta
     1861 aaaaattgtg gatactcgtt tgctaagtgt gctactgtat atcctccagc actggtgccg
     1921 tataaaaaag cgtattgtat gtcgtaagct tattctcttg tacccatttg gcacacacag
     1981 caacatcttc aaaagttttt gttttatttt gcttagctcc tttcttatac cattcatatc
     2041 ctaactctcc acttcctcta accagtggat aagcataaat aaaacctctg tcaagtaatg
     2101 gaaaaatatc gctacgaaaa cccggaagca aagatttacc catgcccgca gcataaacct
     2161 ccattaacat aggtcgagtt tttttgccct ttttcatgcc taccattact attggaattg
     2221 ttattccatc ggaatgtttc gcatttgcaa tttttacttc atatttattt ttattatagc
     2281 ctcttatttc tgtgtgctta gtttgactta taatatcatt aggaatgtca taaatccact
     2341 tttgaggagg ttctaaataa gattcgaaac taagttctgt ttttgtttca cttatatttt
     2401 tgcaaaaaga aactgtatag ttattttcct tccattttaa atgtttaaaa ctaagtgttg
     2461 cctcatcaac aataccaaaa tctgttgtga ttccatgctg aaacgacaaa agtaattttc
     2521 ctgcattaaa attaagttca gttattgttg tatcgctcaa aaatgaaaaa atattttctg
     2581 ctttttttat atcatgctca ttttcaggca aaaccataat cgaaaaattt ggtttgctat
     2641 aatttgttct tatataaaac ttattgttat aatgatcaaa cttagcataa acggaatctg
     2701 gaatattttt gaaagctact atactacaat tctcattttc agtgttaatt gccaaagttg
     2761 aataattatc tgaaaaagaa aaactttcga taaaaataaa tttgttagaa gccgatcttt
     2821 taagagatag tttttttctt tcatcaactt ctttataaat tagagagtca acattacttt
     2881 taatatcatg caaataaact tgtgttggaa aaaagtcatt atcatcaaga gaagtatata
     2941 aaaacctgtt attgtcaatc cattccaatt gcatcacatt ttttaactca caaattttcc
     3001 ccaaattttc tatgtttatg caaattaaat tcaaattcct cccaccatca ggagattcaa
     3061 tataagttaa cacaccagca tcttcgttaa atgaataatc gtaaagtgca tattgactgt
     3121 tttgtatggt tttttcaaca agattaatta cgacttcatt ggcagaagtg tcgggagaag
     3181 aacttcgaca gaaattacct tcaagtgaga aaaaataata ataagaatta atagtatcaa
     3241 aactaaattc gtatttatgc ttcaactcat atgatttaag ttcgttgtaa atacctttta
     3301 tcaagctgtt gttctcatac aaatatgata agacgtattt gttttccttt tcaatataat
     3361 ccaatagctc agaattattc tttgtccacg catacgtgtc aacaatggtg tcgttgtgaa
     3421 tatatgatgt gtcaataaca tttaatgcaa agggtttttc catttcgtca aatgagataa
     3481 atttgctttg atactgatct tccaatgatt ttgttgcttc tattttattt aaaatttcgg
     3541 tttgtaattc tgttgggtac aacatattgt aattattcca aaaatctttg tcataagaag
     3601 ttggataatc ataaagtgaa ttcgaaataa tgtttttata agctaaatta tctgctattt
     3661 tttttacacc ttcagtatta taattattaa aaaccagctc cctatcataa gccatctgaa
     3721 cagtaatatc ttctaacaaa tacttgtatt cataagtatt tattgatttt atatggttca
     3781 aataccaaag ccctttaata tttttaaaat cgaccctaaa attcaactct ttttctgtta
     3841 caatatcatt aacagtcttt ttttgtcctt ttatttgccc tgttctctcc atttgagaag
     3901 aattgttaag agtatatttt ccactataac taattattgc tccactttct ctttcaacaa
     3961 ttactcttaa ccaatcggaa ttctgtttac ttacaaaatt caaatcataa acatttttgc
     4021 catcgtatat actaaaatca atatgatatg tgaatttctt tttatttttc aaagttttaa
     4081 gccctgatga actaaaataa aatgtttcat ttctaaaaaa gtcagtagct gtaatgttta
     4141 acggtccacc agcaatacaa aatttagaaa tactacctgt aaaatcatca ctcgaacgtg
     4201 cgctaattat ttgtactgca tcatctggat acgttgcatt tgtggaataa ttaagtgcct
     4261 gatcgtatcg gtaattacta ttataagcac taaaatcaag ataattccat atagcatcat
     4321 ttctgttaaa ttttgatgca taaggagaaa tataaaaaag acatgctgct tctgcaagtt
     4381 taacaggttc attgttttct aacattattt cacgataaaa tccttcggaa ttataagcat
     4441 taacattgta taaataggac tttttcaata gcgcattttg cagtaactca aatgggtcta
     4501 taggctttac agttacctcg ttaatgaact caatagattt cttcaaacga aaaacatttt
     4561 gtttaaaaaa agctgagtca acaggaattt ttaaattaga atagccaaga aaagaaatca
     4621 acaaagtatc ctttaaatta gttttgttaa ggtaaagtac aaattcacca ttttcatttg
     4681 tcatagttcc aacgggtgaa tttaacaaac taatatttgc atattgtaat ctttcgccag
     4741 tatcaaaatc ttgaatagtt ccagtcaaac tattttgaga gactactgtt attgcattta
     4801 aaagaaacaa taaaacaaat gttattactc tattgcacat aaatttattt aattcaacaa
     4861 taattaaacc tggtttacct ccatattatt ctgataacct atcaaccaca ccacaaactt
     4921 agcgaaaaaa atcaaataat agcatgaaaa tcaaaacctt ttttttgcaa ttgctcatat
     4981 ttttcacctt tataatattt taagcatttt tttatcaaat ctggaatagc ttcaggttct
     5041 tgagtagttt tataatttgc tttatattgt cttatgaaaa tataacatgc catataagga
     5101 gttacttctc cgcgtgtcat agcatttaca attatttttt caaaattatc aacagaaaga
     5161 tgaagcatca acacatctgt aataataatt tcgttataag cattgtgcag ccctattgtt
     5221 aaaaaacctg gataatagtt ttcgctaact atgcgattaa gttttattgt atttacacta
     5281 tccgcaactt tcattttagc ctttttatta aaaacagact ttctagcttt ctgatcgttc
     5341 aaaaacattt cttgtaagta taagtagata gaatcacttt ccatctcaat tctttcacaa
     5401 gcctttatgg aaaccgcttt catttctttc caaaaagttt catttttata aaagttgcta
     5461 tatttatttt taactcgctt atacgacttt cccatttcaa gagatttctg aatgtaattt
     5521 ttcatcaaat caatactatc taaataatat gcaactaaag tggcattatc tatgtcttta
     5581 ggaaaaggtt tatctacagt gttaaatgcg tctttataaa ctactaatgc ggaatcaaat
     5641 tttgtgtcta ataagtaggt ttgtgctttt agaattttaa tattataatc gatataattt
     5701 tttgcaaaaa cattgcaagc acatactatt aaacaacaaa aactattata tttttattca
     5761 aaataattat ttatttttct tattaaccaa caaaatttta aaaacactta tacaattata
     5821 tctataaaac aaaaacgcta aaacctgaat tttagcgttt ttacttacac attattttaa
     5881 ttttatctaa tcagcgtaac attaccagta tatgtatgtt cattaccata attatccacg
     5941 aacttcacta cccatacata cacatctggt gggcattcca tatctccgta tcttccgttc
     6001 cattcttttt cgtagccttc agatttgtgt actatttctc cccaacggtt atatactatt
     6061 agcgagtaag tcttctcgtc aattccatca acaattactt taaatgtttc gttaaggtcg
     6121 tcgtagtttg gtgtaaatgc agtaggagca taaacagcca attcgtttga gacatatata
     6181 tcaacaccag ctgaatcaac gcaaccatat agtgattctg acactaaagt aacatggaat
     6241 gttccaggca tagaataagt gtgagaagga tttgtatcat cactaaagtt tccatctcca
     6301 aagctccatt tgtgccagaa gccgccgtga gtatagtttg tgaaatatat caacggactt
     6361 gccattgtaa catttgttgt atgagctcta aattctactt ctggatttgg gaataccact
     6421 atgcctattg tagagtcgcg tttacaacca tcagtagatg taatttctaa gcctacatta
     6481 aatgttcttg cattatagaa agtatgtttt ggatatttat caaaagataa gttttcaaaa
     6541 tctccgtcat caaaattcca aacatatctt tgtccaacat cagggctatt ttcatagaat
     6601 tgaacttcaa gtggtgaaca tccatttgta ggctctgctg ctatattcat caatggtaaa
     6661 ggatatgttg atattgaaat aggcttagtt atatggttaa atttacaagc atcaaaaact
     6721 tcgaaaataa ttgttgtatc ttggtttatt gcaatgctaa caggtagatt ccataagcta
     6781 tctcctaccc aaacttggtt tggatcaatt ccgccaccac ttataataac ttcaaaatca
     6841 actatatcgc ca
//
LOCUS       DAOPNX010000112         6806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_12323_length_6806_cov_5.645682, whole genome shotgun sequence.
ACCESSION   DAOPNX010000112 DAOPNX010000000
VERSION     DAOPNX010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12323_length_6806_cov_5.645682"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2545)
                     /locus_tag="PKN53_09925"
     CDS             complement(<1..2545)
                     /locus_tag="PKN53_09925"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89307.1"
                     /translation="MKQKLLYITISLVLLVSFSNKTFAICNPTTAGGALTPTTNWQTQ
                     AVNTNTYYTFVANYPGETFIFSFCQGGGSHSLDTQIEIYTSAGVAIPGAYNDDHCGLG
                     SELVFSAPSAGTYRIGIFRYYCNSTVQSAGTLAFKRLPEPTSADCLGALPLCNANNSH
                     PISASGTGNYYDLFSFYDHLGNPNYNNNCPNCLNNGEVHPMWYTFTAQSSGSVNFTIN
                     PVNSADDYDWAIYSLNNGVTCLDLVNYHVAAHRPVRCNFCGDATGATGLSTSGTNTCE
                     GPNTCSNWNLPLNVAAGETYAVIITNFSQSTNGYSINFSGSATIIDHTGPYIDDIVYD
                     PVCGSSSLTLQFSERIWCESMQPADFILTGPGGTYNISDTWSAVCEAAAATSTFAGGT
                     YYDDLWTLELSDYLTQDGNYTLTLLNGGVNDICNNTNSQQTINFTIIGITATVSPTHV
                     ACYGESTGRIDVSTPSGGVPPYTLTWSGPVSIANNNYHPTGLPAGNYTLTITDSNGIC
                     EYVETVTIQQAPLLSYNTNITHPSCAGGSNSGSVLVTGTGGTSPYNIQLGSSTQNGVT
                     SYNFTGLAGGNYNISITDQYGCTASGSVSLNVVEVPDPTFTYNGNQCFVGHSFNFTHT
                     GTPIAGEQYLWTFTGGTPATSTVENPTGVTWASPGTYSVNLRITGGGCVENYSQNITI
                     YPSPTPTLNSTQPSCGLCNGSITVNPAFNSYAWSNSASTQTISNLCPGAFIVTVTDNN
                     SCTATASTTLTNSGNNPTANVVVTDPTCNGLCNGTATVNASGASSFTYHYSSGSTPNN
                     QTTGGLCDGTFDVTVADGSNPACYIVVPFTVTEPSLLQASSSATAIACYG"
     gene            complement(2574..3134)
                     /locus_tag="PKN53_09930"
     CDS             complement(2574..3134)
                     /locus_tag="PKN53_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89308.1"
                     /translation="MKKLVLLFLGILTFSYLFSQEKKLEFVEYKNINDGYTVYFNVAN
                     IENEEQAQLILKDLLAENGISWGRYFKSGDNRDRFQLNIDSKITANYIQNILLTHNVD
                     YDYSNVSVNGVILNPPDKPVAIEAMGSPERKLADDFPVFERTENTEQDIENYRAKKDK
                     WIEENPEKYNQELKELEEKNKWRENN"
     gene            complement(3147..4475)
                     /locus_tag="PKN53_09935"
     CDS             complement(3147..4475)
                     /locus_tag="PKN53_09935"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOD89309.1"
                     /translation="MENTNNNMKNIKKILQNYEAPYDHKDWLRLKKDLPGASKSGGAT
                     KIILVSSVIVATIASIILFINSFDSNKKEITKLETPVVVKETESNIINQKIDEPIIVE
                     QTIPEEPTPHYNYNSFTNTNNENTIKTKQPDTKILVDEDKTKTHINSEQTQAKQTKAE
                     NTYSEKTLITQNKDINNESETVDVNTNTDIDKISFSIEVLDNCVPAKVKFYAENCPKN
                     YEILWNTGENTKISGNKIEYTYLKEGNYQPEVFVILDNFIMKSERLNDIVINKSTNIE
                     INFDNSENSYYFTCDKIDDLDLVWTIENQHFNSKELSYRFDKEGVYMINLTGTNKFGC
                     KSEVSKNIIVAKEQIFFVPNAFTPNSNDINSHFGPIGENLSFASYLLIILDSNGTKVF
                     ESDKPEIMWNGKINNIGNDAKSGIYLWEIRTVDNFGNYQSKKGRVNLIRN"
     gene            complement(4459..5034)
                     /locus_tag="PKN53_09940"
     CDS             complement(4459..5034)
                     /locus_tag="PKN53_09940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD89310.1"
                     /translation="MDLSNADDAKKIVDGCIRGDVKYQQILYKATYGKMLGLCLRYAK
                     DKEEAKDFLHDGFIKVFEKIKFFKHSGSVEGWIRRIIINNTIDIIRRKNRMAFVSDSE
                     NDLMRLTAESDDNLENISNTQANAEIIIGLIQELSPAYKTVFNLYVIEEYTHKEIAEM
                     LNISIGTSKSNLAKAKEKLKDMFIEKYGEYK"
     gene            5398..5826
                     /locus_tag="PKN53_09945"
     CDS             5398..5826
                     /locus_tag="PKN53_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOD89311.1"
                     /translation="MKKYVLKITDKVYNVDVDVNEDVADVSVNGNKYNVLIESVVDDN
                     NTPIIKKATAEQPKSEPVVETKSSSGAKEMITSPLPGNVLKIEVEPNAQVKRGDILII
                     IEAMKMENNILAPHDGIVEKVFVKAGDVVMLGDELISMGK"
     gene            5848..>6806
                     /locus_tag="PKN53_09950"
     CDS             5848..>6806
                     /locus_tag="PKN53_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOD89312.1"
                     /translation="MKSIKKVFLLIFVLAFLIILSPLINAKSVNSADNKQINTTEQTV
                     VPTDNSANSAQDKGFDVKENLIKFTKDSGIYSLFTTNWKALIMIIVSCFLLYLAIVKQ
                     YEPLLLLPIAFGMLLSNMPGGGMFTPELFAEGKIQWADFFAKAGLVDYLYLGVKFGIY
                     PCLIFIGIGAMTDFGPLIANPKSLMLGAAAQLGIFITYFIAIALGFLPQEAGSIGIIG
                     GADGPTAIYLTSKLAPHLLGAIAIAAYSYMALIPVIQPPIMRALTTSKERKIVMKQLR
                     TVSKKEKIIFPIVIAVVTSLIVPSATTLIGCLMLGNLLREAGVV"
BASE COUNT         2191 a         1067 c         1069 g         2479 t
ORIGIN      
        1 cgccataaca tgcaatagct gttgcacttg aactggcttg taataagcta ggttcagtta
       61 cagtaaaagg tactactata taacaagcag gatttgaacc gtctgctaca gtaacatcaa
      121 aagttccatc gcataaccct cctgtagttt gattatttgg agttgaacca ctagaataat
      181 gataagtaaa tgagctagct cctgatgcat ttactgttgc agttccattg cagagtccat
      241 tacatgttgg atcagttaca acaacattag cagtaggatt attgccagaa ttagttaatg
      301 ttgtagaagc agttgctgta caggagttat tatctgttac agtaacgata aatgctcctg
      361 gacataaatt acttatagtt tgagtacttg cactatttga ccaagcatat gaattaaaag
      421 caggatttac tgttattgag ccattgcata aaccacaact tggttgagtt gaattaagag
      481 taggggtggg gcttggatag attgtaatat tttgagaata attttcaaca caaccaccgc
      541 cagttattct taagttaaca gaatatgttc ctggacttgc ccaagtaacg cctgtaggat
      601 tttcaactgt tgatgtagct ggagtaccgc cagtaaaagt ccataaatac tgttcgccag
      661 ctattggtgt tcctgtatgt gtaaaattaa aggaatgtcc aacaaaacat tgattcccat
      721 tataggtgaa tgttggatct ggaacttcta caacatttaa tgaaacacta ccacttgcag
      781 tacaaccata ttggtcggtg atgcttatat tgtaatttcc acctgctaag cctgtgaaat
      841 tatatgatgt aactccattt tgagtagatg agcctagttg aatattatat ggtgatgttc
      901 cgcctgttcc agttactaaa acagaaccac tattactacc acctgcgcac gaaggatggg
      961 ttatatttgt attataactt aaaagaggtg cttgttgtat agttacagtt tctacatact
     1021 cacaaatacc attggaatct gtaatagtga gagtgtaatt tcctgccggc agtcctgtag
     1081 gatgataatt attattggca atagatactg gtcctgacca agttaaagta tatggaggta
     1141 ctcctcccga aggtgtacta acatcaattc gccctgttga ttctccgtag caggctacat
     1201 gagtaggtga aacagtcgct gttattccaa tgattgtaaa attaattgtt tgttgagaat
     1261 ttgtattgtt acaaatatca tttacaccac catttagaag agttaaagtg tagtttccat
     1321 cttgtgttag ataatcagat agttctaaag tccataaatc atcataataa gtaccacctg
     1381 caaatgttga tgtggcagct gccgcttcgc atacagcact ccaagtgtct gaaatgttgt
     1441 aagtaccacc aggacctgtt aatataaaat cagcaggttg catcgattca caccatatgc
     1501 gttcagaaaa ttgtaaagtt aaagatgaac taccacaaac aggatcatat actatatcat
     1561 caatatatgg tcctgtatga tcaataattg ttgcactacc actaaaatta attgagtagc
     1621 cgtttgttga ttgactaaag ttagtaatga ttacagcata agtttctcct gcggctacat
     1681 ttaatgggag gttccaatta gagcaagtat ttggtccttc acatgtgttt gttccactag
     1741 tgcttaaacc tgtcgctcca gtagcatctc cgcaaaaatt acatctaaca ggtctgtggg
     1801 cagcaacatg gtaattaaca aggtctaaac aagtaactcc attatttagg ctataaatag
     1861 cccaatcgta gtcatctgca gaattaacag gattaattgt aaaattaaca ctccctgatg
     1921 attgtgctgt aaatgtgtac cacataggat gtacttcgcc attatttaaa cagtttgggc
     1981 aattattgtt atagtttggg tttcctaaat ggtcataaaa actaaaaaga tcatagtagt
     2041 tgcctgtacc agatgcagaa attggatgtg aattatttgc attacataat ggtaaagcac
     2101 ctaaacagtc ggcagaggtt ggttctggaa gacgtttgaa agctaaagtt ccagcagatt
     2161 gtacagtaga attacaataa tacctaaaaa tacctattct atatgtccct gcactaggtg
     2221 cagaaaaaac aagttcagat ccaagtccac agtggtcatc attataagct ccaggtattg
     2281 ctacaccagc acttgtgtaa atttcaattt gagtatctaa tgaatgagag ccgccacctt
     2341 gacaaaatga aaatataaat gtctctccag gataattggc tacaaatgtg taataagtgt
     2401 tagtattaac tgcttgtgtc tgccagttag tagttggagt taaagcacca ccagctgttg
     2461 tagggttaca aatagcaaaa gttttattgc taaaactcac taataaaact aaacttattg
     2521 taatatataa aagtttttgt ttcatgatta ttatattagg cttaaattta tacttaattg
     2581 ttttctctcc acttattttt ttcttctaat tcttttaact cttgattata tttttcagga
     2641 ttttcttcta tccatttgtc ttttttagct ctgtaatttt caatatcttg ctcggtattt
     2701 tcggttcttt caaaaacagg aaaatcatca gctaatttcc tttctggact ccccatagct
     2761 tcaatagcca caggcttgtc gggtggattt aatattacac cattcacact tacgtttgag
     2821 tagtcataat caacattatg tgttaataaa atattttgaa tgtagttagc ggttattttg
     2881 ctgtcaatat ttaattgaaa tctatctcta ttgtctccag atttaaaata tcttccccaa
     2941 cttattccat tttcagcaag caagtctttt aaaattaatt gagcttgttc ttcattttct
     3001 atattagcta cattaaaata gacagtatat ccatcattta tgtttttata ttcaacaaat
     3061 tccaactttt tttcttgtga aaataaatac gaaaatgtta aaatccctaa aaataataaa
     3121 actaattttt tcatattgtt tgttttttaa tttctaatca aatttactct ccctttttta
     3181 ctttgataat ttccaaaatt atctacagtt cttatttccc ataaataaat tccagacttt
     3241 gcatcatttc ctatattatt tattttccca ttccacataa tttcaggttt atctgattca
     3301 aaaactttag tcccatttga atctaatatt attaataggt atgaagcaaa tgataaattt
     3361 tcaccaatag gtccaaagtg cgagttaatg tcattgctgt ttggtgtaaa ggcattagga
     3421 acaaaaaata tttgttcttt tgcaacaatg atgtttttag aaacttcaga tttgcaacca
     3481 aatttattag ttccagttaa atttatcata taaacacctt ctttatcaaa acgataactt
     3541 agctctttgc tgttaaaatg ttgattttca atagtccata ctaaatccaa atcatctatt
     3601 ttatcacatg taaagtaata tgaattttct gaattatcaa aattaatttc aatatttgtt
     3661 gatttattta ttacaatatc attaagtctc tctgatttca ttataaaatt atccaaaatc
     3721 acaaacactt caggttgata attaccttct tttaaataag tatattctat tttattgcca
     3781 gaaatttttg tattttctcc agtattccat aaaatttcat aattcttagg acaattttct
     3841 gcataaaatt tcacttttgc aggaacacaa ttatcaagta cctcaataga aaatgatatt
     3901 ttatctatat ctgtgtttgt gttcacatct acagtttctg attcgttatt tatatctttg
     3961 ttttgagtta ttagtgtttt ttcagaatat gtattttctg ctttagtttg tttagcttgt
     4021 gtttgttcag aattaatatg agttttggtt ttatcttcgt caactaaaat ttttgtgtca
     4081 ggttgttttg ttttaatggt attctcgtta ttagtattag taaaggaatt gtaattataa
     4141 tggggtgtag gttcttccgg aatagtttgt tctacaataa tcggttcgtc aattttttga
     4201 tttattatgt tgctttcagt ttcttttact actacaggag tctcaagttt ggtaatttct
     4261 tttttattcg aatcaaaaga attaataaac aaaattattg aagcaatagt tgcaacaatg
     4321 acgctactta ctaatattat tttagtagca ccacctgatt tagaagctcc tggcaaatct
     4381 ttttttaatc ttagccaatc tttgtgatcg taaggtgctt catagttttg taatattttc
     4441 tttatatttt tcatattatt atttgtattc tccatatttt tcaataaaca tatcttttaa
     4501 cttctctttt gctttggcaa ggttggattt tgatgttcct atgctaatat taagcatttc
     4561 cgcaatttct ttatgcgtgt attcttctat aacatatagg ttaaaaacag ttttgtatgc
     4621 tggcgacaat tcttgaatta gtccgataat tatttctgca ttagcttgtg tattactaat
     4681 gttttcaagg ttatcatcag attcagcagt caatctcatt aaatcatttt cactatcgct
     4741 aacaaatgcc attctgtttt ttctccgaat aatatcaata gtattattaa taattattct
     4801 tctaatccat ccctcaacag agcctgaatg tttaaaaaat ttaatttttt caaagacctt
     4861 tataaatccg tcgtgcaaaa aatcttttgc ttcttcttta tcctttgcat atcttaaaca
     4921 tagaccaagc attttgccat aagtagcttt ataaagtatt tgctgatact ttacatcacc
     4981 acgaatacat ccatctacta ttttctttgc atcatcagca ttacttaaat ccatacctta
     5041 agatttcttt taattgacgt attaatatta atttagttgt cttttttact aatttttcct
     5101 atttattttt tctgtataaa aataatattt ttttttgaaa aatatatgaa aaatgtgttt
     5161 taaaaaagtt tgtgtgtaaa ttgtttttta ttatgtttgc aacacttaaa aattattgat
     5221 tatagaacta gttcataaaa atatcgagta tgttataatt gccgaaaaat atatgctcaa
     5281 ggcaagaaaa tatcatgaaa gaattttaga tttaaaattc tttgaaaata agtccttact
     5341 gctaacaata aaaaatacag aaaactcaaa atggaataat tttaactact atacacaatg
     5401 aaaaagtatg ttttaaaaat tactgataaa gtctataacg tggacgttga tgtaaatgaa
     5461 gatgttgctg atgtgtcggt caatggtaat aaatataatg tgttgattga aagtgttgtt
     5521 gatgataata atacaccaat tataaaaaaa gctactgccg aacaaccaaa aagcgagcct
     5581 gtagttgaaa ctaagagttc tagtggtgct aaagaaatga taacatcacc attgccaggc
     5641 aatgttctta aaatagaagt agaaccaaac gctcaagtga aacgtggcga tattttaata
     5701 attattgaag caatgaagat ggaaaacaat atacttgcac ctcatgatgg aattgtagaa
     5761 aaagtttttg ttaaagctgg tgatgttgta atgttgggcg atgaactcat aagtatggga
     5821 aaataaaaat ataaattaat gattgatatg aaatcgataa aaaaagtttt tcttttaata
     5881 tttgtacttg cttttttaat aattttaagt ccgctaataa atgccaagtc tgttaattct
     5941 gctgataata aacaaataaa cacaaccgag caaacagtgg taccaactga taattcagca
     6001 aattcagctc aagacaaagg ttttgatgtt aaagaaaatc tgataaagtt tactaaagat
     6061 tcaggtattt attctttatt cacaacaaat tggaaagctc tgataatgat tattgttagc
     6121 tgttttcttt tatatttggc gattgtaaaa caatatgaac ctcttttatt attgcctatt
     6181 gcatttggaa tgttattgtc gaatatgcca ggtggtggaa tgtttactcc tgaactattt
     6241 gctgaaggca aaattcaatg ggctgatttt tttgcaaagg caggtttggt tgactattta
     6301 tatctcggtg taaaatttgg aatttatcca tgtttaattt ttatcggtat tggtgcaatg
     6361 actgattttg gaccacttat tgcaaatcca aaaagtttaa tgttaggtgc tgctgctcaa
     6421 ctaggtattt ttattactta ttttattgca atagcacttg gatttttacc tcaagaagct
     6481 ggttcaattg gaattattgg aggagccgac ggaccaacag ctatttatct aacaagtaag
     6541 ttagcaccgc atcttttggg agcaattgca attgctgctt attcatatat ggctttaata
     6601 cctgttattc aaccaccaat tatgcgagct ttaacaacaa gcaaagaaag aaaaatagta
     6661 atgaaacaac taagaactgt atctaagaaa gaaaaaatta ttttcccaat tgtgatagca
     6721 gttgtaactt cattaatcgt accctcagct actacattaa ttggttgttt gatgctcgga
     6781 aacttattaa gagaagcagg tgttgt
//
LOCUS       DAOPNX010000113         6551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_12899_length_6551_cov_6.254156, whole genome shotgun sequence.
ACCESSION   DAOPNX010000113 DAOPNX010000000
VERSION     DAOPNX010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6551
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12899_length_6551_cov_6.254156"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..596)
                     /locus_tag="PKN53_09955"
     CDS             complement(93..596)
                     /locus_tag="PKN53_09955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD89313.1"
                     /translation="MESKFLKSILVFGIFALYVLPISAQNLYVRNANETQEIYQLSSI
                     SKLSFSASDVIVHKTDNSIATHAISAIRYMSFNNFMGIYLPREQENMLTVYPNPASNL
                     LNINLPSDKLGGDLLVLSLDGKTLMSYKLVDSQTAIDLNTLPQGVYFCRYIYGTHSET
                     VKIVKQN"
     gene            complement(837..2495)
                     /locus_tag="PKN53_09960"
     CDS             complement(837..2495)
                     /locus_tag="PKN53_09960"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOD89314.1"
                     /translation="MVMNEKLAARIEEIAVRLTNQLSVVETAGELNAVQEVHNIFMEM
                     DYYKKNPQDLQWVHVPNDKLGRKSLIARMRGGKGNNKKTIVMIGHIDTVGISDYGVLK
                     DFAHRPYELTEEFKKVASTLPPEARKDLESGDYLFGRGLFDMKTGDAIIMAVMEEIQK
                     DIENFEGNLIYAAVCDEESNSFGMLSVVPELVKIRDAEGLDYLALLDTDYMTSEYEGD
                     ENKYVYIGTVGKLMPSFYVVGKETHVGESFKGIDANQIASAITCRINLNADFCDVAEG
                     EVSLPPITLKQRDLKPEYSVQTVQYANLFFNYATHTSTPDEVLEKMINAADEAFQNSI
                     DNLNTQYERFCKLANREYKKLPWKSRVISYEELYNTVKAELGDELDAKIKELSDKMLK
                     DESIDQRDFALKIVELTHHLWSDREPIVIVYFTPPYYPHIYVEGKRPKEKALLEAVAD
                     AVNTTKSDYKLVYKKFFPYISDLSYAAAPKDPKIIENFKNNMPGFGTKYDLPLEDMQK
                     LDLPVLDIGAFGKDAHRFTERIEKKYSFEVAPVLVYKTIMNLLK"
     gene            complement(2635..3963)
                     /gene="rmuC"
                     /locus_tag="PKN53_09965"
     CDS             complement(2635..3963)
                     /gene="rmuC"
                     /locus_tag="PKN53_09965"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOD89315.1"
                     /translation="MEALFLVLGIAKGFLIAWLIFRRKLKLAKQSEISMQELIQAKSD
                     LTKYETENNFLIKQLEETKTSFSNKDSENKELIAKLSIAETNLSNLNEKLETNKAELE
                     GLHKKFTLEFENLANKVLEEKSQKFSELNKKQIDAIINPFNENLKEFKQTVKDTYEKG
                     MKERTELGAELKHIQELNQKLSVEANNLTKALKGDVQKQGRWGEMILEKILESSGLEK
                     DKHFRTQDSFTLSDGRRQRPDAVVYLPENKHIIIDSKVSLVAYEKYVNSDNEEEKKQF
                     LKEHITSVRAHIKDLADKDYINSLGLENPEYILMFVPIEASLGLTVSNDNQIFTEAWD
                     KRVVIVSPSTLIATLMTVNAIWKQTMQTENALLIADRGGKLYEKFIGFVDDLKAVGVS
                     IEKANKEYEKAMSKLSEGSGNLVRQAEMLKELGAKTTKSIDKEILELGKE"
     gene            complement(4026..6140)
                     /locus_tag="PKN53_09970"
     CDS             complement(4026..6140)
                     /locus_tag="PKN53_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89316.1"
                     /translation="MRKLVFVVVMSLVVNCLYSQNRDLLIEHVELLNTTGFFTSKKDT
                     KLVNGEKQLIFEFTDVTEEYIVRVFPAKKFSDLRFDFFSTSDYEVIDSVFFIDDSFYQ
                     TKIRFKKIIDNPQLSLKVRSFTKEGRAMITEVKLFPVAVMDYGFVQKPEDMFIGEENF
                     IEIATNLPKNIVLQANWDTDYPLHTRIVNHKGNVAININATKTGQMQVPVFLKLKRPV
                     RDSLGKFSYEIGPLNVEFNVKAGRLAFLNTDIKDISVDEKNRYEGVEIQIDNNPNLQI
                     QKTYRLVESEEPGAALVGELFTKARLTNNRVLCVLRAFNYHNQTDGVLYINDGEIPKF
                     ITNFSIIPLAKIENFEIMRTGGEWTKSSVVYPGEEIIVRLEGQSLSKAKFAIEDLIIS
                     KEDTLVNRNEVVELRAKVPINIRKKNLNILINNSLSGKSLNVAEYEEFRPLDYIQIDY
                     GNGPKNLFDFTGPEFSTKHLKDLVISFDNDKIDENEKFYGNQYFDLEVKITGSKGEIL
                     EILTLNSNKVSPGSASPRHQYYNKKNAINEIRLNQFLSRKTFDLDTWHRIQLVFKPTK
                     ATGNRSDTKIVDIIVQKSMRFDIDVSFPAGLITKKVGEPGYGDLGGISLAMIAQFSFY
                     QKDKIARFKPYKIGAGFIAINALNFADSNITRDVGVVLIGSLYPTTKETRMTFPLYLG
                     GGYLLSTEKWFFLLGPGIRVRL"
     gene            6337..>6551
                     /locus_tag="PKN53_09975"
     CDS             6337..>6551
                     /locus_tag="PKN53_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89317.1"
                     /translation="MRKHFWSIWLILLFTSGSLLAQNSTFNKDSVFTWGMNEFANGNV
                     EVALQHFETYLENVTDSTTAQYADVLN"
BASE COUNT         2108 a         1093 c          804 g         2546 t
ORIGIN      
        1 aaacaaaata aataaatgac aaaattaaaa ttttgaattt aacgaatatc cctaattaat
       61 ataagttttt tcatttttat ttcctcctat ttttagtttt gcttaactat ttttactgtt
      121 tcactgtgtg tgccataaat ataacggcaa aaataaacac cttgtggcaa agtatttaag
      181 tcaatagctg tttggctatc aacaagtttg tagctcatta aagttttacc gtctaaacta
      241 agcacaagta aatcaccacc aagtttatcg cttggcaaat taatatttag caagttactt
      301 gcaggatttg gataaactgt taacatattt tcctgctctc ttggcaaata aatgcccata
      361 aagttattga aactcatata tcttatagca ctaatagcgt gtgttgctat actgttgtcg
      421 gttttatgta caataacatc gctagccgaa aagcttagtt tgctaatgct actaagttgg
      481 taaatctctt gagtttcatt ggcattacgc acatatagat tctgtgccga aatgggcaaa
      541 acatataatg caaaaatacc aaatactaaa attgatttta aaaatttact ttccataata
      601 aagaattaat tgttaaacaa aattatttta ctaaaataaa cctttttttt tacaagtatc
      661 tatttcataa aacaaatata caatattttt aatataaaaa taaaaaaatg attttttaaa
      721 ttattaataa acaattttca gagcgagcga gattatgttt tgaattagca taaaaaaagt
      781 agagacgcta aatattacaa cgtctctacc ctatattatt tattttctta taatttttac
      841 tttaacaaat tcattatagt tttatagacc aaaacaggtg ctacttcaaa agaatatttc
      901 ttttcaattc tttcagtaaa tctgtgagca tctttaccaa aagcgccaat atcaagcact
      961 gggaggtcta atttttgcat atcttcaaga ggcaagtcgt attttgttcc aaatccaggc
     1021 atattatttt taaagttttc aataattttt ggatcttttg gagctgctgc ataacttaaa
     1081 tctgaaatat atgggaagaa ttttttgtaa accaatttat aatctgattt tgttgtattt
     1141 acagcatctg caactgcttc taaaagagct ttttcttttg gtcgttttcc ttctacataa
     1201 atatgaggat aataaggagg agtgaaatac actataacta taggctctct atcagaccat
     1261 aaatgatgtg taagttcaac aatttttagt gcaaaatctc tttggtcaat gctttcgtct
     1321 ttaagcattt tgtctgaaag ttctttaatt tttgcatcaa gctcatctcc aagttctgct
     1381 ttaactgtat tataaagctc ttcataagat attactctcg atttccaagg taattttttg
     1441 tattctctat ttgcaagttt acaaaatctt tcatattgag tattcaaatt atcgatagag
     1501 ttttggaaag cttcgtcagc tgcattaatc attttttcta aaacttcgtc tggcgtgctt
     1561 gtatgagttg catagttaaa gaataaatta gcatactgaa cagtttgaac agaatattct
     1621 ggttttaaat ctctttgttt aagtgtaatt ggtggaagag aaacttcgcc ttctgctaca
     1681 tcgcaaaaat ctgcattcaa atttattcta caagtaattg cagaagcaat ttggtttgca
     1741 tctatacctt taaacgactc tcccacgtga gtttctttac ccacaacata gaatgaaggc
     1801 attagttttc ctactgttcc gatatataca tatttatttt catcgccttc atactctgaa
     1861 gtcatataat ctgtatctag aagtgctaaa taatctaatc cttcagcatc tctaattttt
     1921 actaattcag gaactaccga aagcattcca aaagagttac tttcttcgtc gcaaacggct
     1981 gcataaatta agtttccttc aaaattttca atatcttttt gtatttcttc cattacagcc
     2041 ataattatag cgtcaccagt tttcatatca aacagaccac gtccaaataa gtagtcgcca
     2101 gattctaaat cttttcttgc ttctggtggt aaagttgatg ctactttttt aaattcttcg
     2161 gttaattcat aaggtctatg agcaaaatct tttaaaactc cataatcaga aatacctaca
     2221 gtatcaatat gtccaatcat tacaatagtt tttttattat ttccttttcc gcctctcatt
     2281 ctggctatta aagattttct gccgagttta tcgtttggaa catgtaccca ttgtaaatct
     2341 tgtggatttt ttttgtaata atccatttcc ataaaaatat tatgcacttc ttgcacagca
     2401 tttaattctc ctgcagtttc tacaacacta agttgatttg taagtcgaac agcaatttct
     2461 tcaattcttg ctgctaattt ttcgttcatt accatacctt aaataagttt tttatttagt
     2521 tattattcat tattttaaaa cactttgagc aagtgcaaat ataatattta aaaggagaat
     2581 ttaaaattta ttgggaaata tttcataaca tgtattttgc tttagcttat atttttattc
     2641 tttaccaagt tctaaaattt ctttatctat agattttgtt gttttagcgc ctaactcttt
     2701 tagcatttct gcttgtctaa caagatttcc cgaaccctct gaaagtttag acattgcttt
     2761 ttcatattct ttatttgctt tttctattga tactcctacg gcttttaaat catcaacaaa
     2821 acctataaat ttctcatata attttccacc tctatctgca attaaaagag cattttcggt
     2881 ttgcatagtt tgtttccaaa ttgcatttac tgtcatcagc gttgcaatta atgttgacgg
     2941 acttactatt acgacacgtt tatcccaggc ttcagtaaaa atttgattat cattagaaac
     3001 tgttaaacct aaagatgctt ctataggaac aaacatcaaa atatattctg gattttctaa
     3061 tcccaaacta tttatataat ctttatctgc caaatcttta atatgcgctc ttacagaggt
     3121 aatatgttct tttaaaaatt gttttttttc ttcttcgtta tcgctattca catatttttc
     3181 ataagccaca agcgaaacct tagaatctat aataatatgc ttattttcag gcaaatacac
     3241 tacagcatca ggtctttgtc ttctgccatc ggaaagagta aacgaatctt gagttctaaa
     3301 atgtttatcc ttttcaagtc ctgatgattc tagtattttt tctaaaatca tttcgcccca
     3361 acgcccctgc ttttgcacat cgccttttaa agctttggtt aaattatttg cttctacact
     3421 aagtttttga tttaattcct gaatatgttt tagctctgct cccaattcag ttctttcttt
     3481 catgcctttt tcataagtat cttttacggt ttgcttaaac tcttttaaat tttcattaaa
     3541 aggatttatt atagcatcaa tttgtttttt atttaattct gaaaattttt gggatttttc
     3601 ttctaaaact ttattagcaa gattttcaaa ttctaatgta aattttttgt gcaatccttc
     3661 taactctgct ttattagttt ctaatttttc gtttaaattg cttaaattag tttccgcaat
     3721 acttaattta gctataagtt ctttattttc actatcttta ttagaaaaag aagttttagt
     3781 ttcttctaat tgttttatta aaaaattatt ttctgtttcg tattttgtta aatcactttt
     3841 agcttggata agttcttgca tactaatttc gctttgtttt gctagtttta gttttcgtct
     3901 aaaaattagc catgcgatta aaaatccttt tgcaataccg agtactaaga ataaggcttc
     3961 cattttttta attttttaaa atgtaaacat aatattttaa agctaattaa tcaagttttc
     4021 aaattttaaa gtctaactct aattcctggt cctaacaaaa agaaccattt ttctgttgat
     4081 aataaatatc cacctcccaa atacagtgga aatgtcattc ttgtttcttt tgttgttgga
     4141 tacaaacttc caataaggac tactcccaca tctcttgtaa tattagaatc ggcaaaatta
     4201 agagcattaa ttgcgataaa tcctgctcct attttatatg gtttaaatct tgctatttta
     4261 tctttttgat aaaagctaaa ctgtgctatc atagctaaag atatacctcc caagtcgcca
     4321 tagccaggtt ctccaacttt ttttgtaatc aatcctgctg ggaaagaaac atcaatatcg
     4381 aaacgcatag atttttgaac aataatatcc acaatttttg tatcacttct atttcctgtg
     4441 gctttcgttg gcttaaacac aagttgaatt ctatgccaag tatctaaatc aaaagtttta
     4501 cgacttaaaa attggtttaa tctaatttcg ttaatagcat ttttcttatt ataatattga
     4561 tgtcgtggcg aagctgaacc tggactaacc ttatttgaat ttagtgttaa tatttctaat
     4621 atttcgcctt tactacctgt gattttaact tctaaatcaa aatattgatt tccataaaac
     4681 ttttcgtttt catctatttt atcattatca aaacttataa ctaaatcttt taaatgttta
     4741 gttgaaaatt ctggacctgt aaaatcaaac aagttttttg gaccattacc ataatctatt
     4801 tgtatatagt ctaaaggtct aaattcttca tattcagcaa catttaaaga ttttcccgac
     4861 aacgagttat tgattagtat attcaaattt ttcttacgaa tattaattgg tactttagct
     4921 ctcaattcta ctacttcatt tctatttacg agagtatctt cttttgaaat tattaaatct
     4981 tcgatagcaa atttagcttt tgacaatgat tgtccttcta atctcactat aatttcttcg
     5041 ccaggataaa ctacagaaga ttttgtccat tcgccacctg ttctcattat ttcaaaattt
     5101 tctattttag ctaacgggat aatagaaaaa ttagtgataa attttggaat ttctccatca
     5161 ttaatataca aaactccatc ggtttgattg tgatagttga aagctctcaa aacgcacaat
     5221 actctattgt ttgtaagtct tgcttttgta aacaactctc ctactaaagc agctcctggt
     5281 tcttctgatt caaccaacct ataagttttt tgaatttgta aatttggatt attatcaatt
     5341 tgaatttcaa caccttcgta acgatttttt tcatctacac taatatcttt aatatcagta
     5401 tttaaaaaag cgagacgacc agcttttacg ttaaactcca cattcagcgg acctatttca
     5461 tagctaaact ttcctaaact atcgcgaaca ggacgcttta atttcaaaaa aacaggaact
     5521 tgcatttgcc cagttttagt tgcatttata ttaattgcca catttccttt atgatttaca
     5581 attcgggtgt gtaaaggata atcagtatcc caatttgctt gtaaaacaat attttttggt
     5641 aaatttgtag ctatttcaat aaagttttct tctcctataa acatatcttc tggtttttga
     5701 acaaaaccat aatccataac agccacgggg aataatttaa cctctgtaat cattgctctt
     5761 ccctctttag taaatgacct tacttttaag cttagttgcg gattgtcaat tattttttta
     5821 aatcttattt ttgtttgata aaaactatca tcaataaaaa atacagaatc aattacttca
     5881 taatcgctag ttgaaaaaaa atcgaatctt aaatcagaaa acttttttgc aggaaaaact
     5941 cttacaatat attcttctgt aacatctgta aattcaaata ttaattgttt ttcgccattt
     6001 acaagttttg tatctttttt tgaagtaaaa aaacctgttg tatttaatag ttcgacatgt
     6061 tcaattaata aatctctatt ctgagaataa agacaattaa ccactaaaga cattacaacc
     6121 acaaaaacaa gttttctcat aatttcaatt ttaaattttt gcaaatttaa ttaaaaaaac
     6181 aacaaaatgg gctatcacaa aacaaacctt cattcaaaaa acaattcttt tctttctttt
     6241 tcaaataaat taatttagtt ttacatttat aaaaaaaata catattttga atatgaaata
     6301 aaaaagttat aacacaaatt ttaacttaaa tcaaatatga gaaagcattt ttggagcatt
     6361 tggctgatat tactatttac ctcagggagt ttgctagcac aaaatagtac atttaacaaa
     6421 gattctgttt ttacatgggg catgaatgag tttgcaaatg gcaatgtgga agtcgcgttg
     6481 caacattttg aaacttattt agaaaatgtt acagatagta caacagcaca atacgcagat
     6541 gttcttaata a
//
LOCUS       DAOPNX010000114         6525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_12964_length_6525_cov_6.929830, whole genome shotgun sequence.
ACCESSION   DAOPNX010000114 DAOPNX010000000
VERSION     DAOPNX010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6525
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12964_length_6525_cov_6.929830"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1142)
                     /locus_tag="PKN53_09980"
     CDS             complement(57..1142)
                     /locus_tag="PKN53_09980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD89318.1"
                     /translation="MKPKRIIVSVSNDLSIDQRVDRICNSLCEWDYEVLLVGRKLKLS
                     KNLDERNYKTKRFKLLFEKGVLFYTCLNIRLFFYLLFSKADIFLSNDLDTLPANALAS
                     FFRNKKLVYDSHELFTEVPELENKKFKKKIWGFLEKISFKRIDKAYTVCESIADFYFK
                     KYGIKMSVVRNLPTLKNITNKFENRENIIIYQGALNKERGINIMIKAMQHIEKAKLII
                     AGSGDLEQELHLLSKDLNLESKVEFTGKLNFNDLFEQTQRAKLGLSLEQGKSLNYYYA
                     LPNKIFDYIQAGVPVICSDFPEMKIIVDNYKVGISSKITDEKTLAKTIIELLDNEQVL
                     NNFHQNCIKAREELNWENEKEVLKDLF"
     gene            1226..2239
                     /gene="murB"
                     /locus_tag="PKN53_09985"
     CDS             1226..2239
                     /gene="murB"
                     /locus_tag="PKN53_09985"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOD89319.1"
                     /translation="MQIFKNCSIKKYNTFGIDVKASVLVEYDHDDEIIDFLKSNQDKI
                     LKLLVVGGGSNLLFQNDFDGLIIHPVGQRMEVIKEDENFIFIKASAGYVWDKFVEDTL
                     NSKAYGLENLSLIPGTVGASVVQNIGAYGVEAKDFVDEVECLDLNSYEIKTIKKFDCK
                     FAYRNSIFKNQLKDKVLVLNVIYKLFKQPKLNCEYTDIQKYFNGKTDVSAKDVREAVI
                     NIRNFKLPDPEQIGNAGSFFKNPIINQEKFDILIKKYEDLRFYNLPNGNYKLAAAWMI
                     DKLGFKGFKHKGAAVHKNQALVIINETGEATGSDICELAEIIKEKVFQNFYVLLEPEV
                     IYV"
     gene            2345..2417
                     /locus_tag="PKN53_09990"
     tRNA            2345..2417
                     /locus_tag="PKN53_09990"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2378..2380,aa:Gly,seq:gcc)
     gene            2447..2532
                     /locus_tag="PKN53_09995"
     tRNA            2447..2532
                     /locus_tag="PKN53_09995"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2481..2483,aa:Leu,seq:taa)
     gene            2671..3570
                     /locus_tag="PKN53_10000"
     CDS             2671..3570
                     /locus_tag="PKN53_10000"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOD89320.1"
                     /translation="MKKTLILTLILSFACINVFSQEEAEQKKGEWKKGGMMSLNASQV
                     SFTNWAAGGQNSIAANSFVNLFANFKKNRMTWDNTLDLGYGKLFQGIKEHKTVYKTDD
                     KIDFSSKYGRYAFEHWYYAALIGFKTQFDQGFKNPTDTVKISDFFSPAYLNLSIGMDY
                     KPCDNFSLLISPLTGKVTFVIDEYLSSIGAFGVDPGKTFRPEFGGYIKAQYKVNFLKN
                     MTYSTKIDLFSNYLEKPQNIDVNWENLVMMKINNWFSASIHYTMIYDDDVNVKWIDAD
                     NIEHFSPKLQIKQMIGVGLTYKF"
     gene            3589..3999
                     /gene="mscL"
                     /locus_tag="PKN53_10005"
     CDS             3589..3999
                     /gene="mscL"
                     /locus_tag="PKN53_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOD89321.1"
                     /translation="MLKEFRDFAIKGNLIDMAIGIIIGAAFGKVVASLVADVIMPPIG
                     LLIGGVNFTDLTIVLKEAVGDNPAVTLNYGNFIQILIDFLIVAMAIFIVIKGINALKR
                     KEEKEPEPEPEPEPTKEEILLTEIRDLLKEGRKE"
     gene            4157..4990
                     /gene="lgt"
                     /locus_tag="PKN53_10010"
     CDS             4157..4990
                     /gene="lgt"
                     /locus_tag="PKN53_10010"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOD89322.1"
                     /translation="MFNYFNYIIWDVGPILVDLGILQIRAYSLFFALGFVVSYFIIKR
                     YYKQEGVPIRELDTLTVWVVVGGLLGARLGHCIFYDWEYYSKNLLEILLPVSFSPKFE
                     FIGYQGLASHGGAIGIIIAILIYYRKSTKKSFFWLIDRIAVPTGFAGALIRLGNLFNS
                     EIYGHQTDLPWGFVFIRNGDTFPSHPTQLYEAICYIITSIVLMRLYKVERFRNAGGFL
                     LGVFFIMVFTARFFIEFVKENQVAFEDSMALNMGQILSIPVVVAGIFLVVRALKLSKA
                     K"
     gene            5001..>6525
                     /locus_tag="PKN53_10015"
     CDS             5001..>6525
                     /locus_tag="PKN53_10015"
                     /inference="COORDINATES: protein motif:HMM:NF020677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PNGase F N-terminal domain-containing protein"
                     /protein_id="HOD89323.1"
                     /translation="MLKQISLLFLLLLPWLSFSQTYKITYKSYSNGKYQEDGDFSIYF
                     KENIVYLSSQNAKVQQFTDFNKKENSKIMEFENNLYRNSTAFSELLKPENIENSTETI
                     LGYKCKLAVFYSFSNKIEVWFTDKAPAYGNPYSAFIPEEKSLALKVVVNGNREIIASE
                     IQKVKNIELPEYQYDKAVKISDSEFEEKVILSRYSRLSIFENAQINFDTDIEQINLEN
                     TEPDVVYHLSNGGIVLKKVRIDESFKNSYFYVELDVKSNGDAYDRTGSVFMLPDNDDF
                     RPMLKAIFGDKSEMPVFEDKYGEKYQGMIATSEFLPQIELMRFFTSFGAGHFNTLRPI
                     NNYPWAEKITYKQDVSQLLPDDEYVWFGVFIGNYDKGGHIINLKLSFYPSFGSADENV
                     QKFILPVFNTVNILEMSGQNYGKFFKSDTLVSEFTVPENLENPVLLYTSTGHGGWGSG
                     DEFVPKMNKIFIDDKEIYSFIPWRYDCATYRFSNPASGNFSNGLSSSDLSRSNWCPGT
                     "
BASE COUNT         2268 a          790 c         1093 g         2374 t
ORIGIN      
        1 aagtatttat atgttttttt ttcaatttaa attacctaat tttttagggt ttgggtttaa
       61 aataaatctt ttaaaacttc cttctcattt tcccaattta attcttctct tgctttaatg
      121 cagttttggt ggaaattgtt taaaacttgc tcattatcaa gtagttcgat tattgttttt
      181 gccaatgtct tttcatctgt aatttttgag gaaatgccaa ctttataatt atcaacaata
      241 attttcattt caggaaaatc tgaacaaatc acaggaacac cagcttgtat ataatcaaaa
      301 attttatttg gaagagcata ataataattc aaacttttac cttgttctaa tgaaagtcca
      361 agttttgctc tttgtgtttg ttcaaataag tcgttgaagt ttagtttgcc agtaaattca
      421 actttagatt ctaagtttaa gtctttgctt agcaaatgta attcttgttc taaatcgcca
      481 gaaccagcaa ttattagttt tgctttttct atgtgttgca tggcttttat cataatgtta
      541 attcctcttt ctttatttaa agcaccttga taaatgataa tgttttcgcg attttcaaat
      601 ttgttagtaa tatttttaag cgttggtaaa tttcttacta cagacatttt aattccgtat
      661 tttttaaaat aaaaatctgc aatagattca caaacagtat aggctttgtc aattctttta
      721 aaagaaattt tttctaaaaa cccccatatt tttttcttaa attttttatt ttctaattca
      781 ggaacttcgg taaaaagctc gtggctatcg taaactaatt ttttatttct aaaaaaactt
      841 gcaagagcat tagcaggcaa tgtgtctaag tcatttgaaa ggaaaatatc agcttttgaa
      901 aataataaat agaaaaacaa tctaatattg agacaggtgt aaaataaaac gcctttttca
      961 aataaaagtt taaatctttt tgttttgtaa tttctttcat caagattttt gctgagtttg
     1021 agctttcttc caactaataa aacttcataa tcccattcgc aaagactatt acaaattcta
     1081 tcaacccttt ggtctatgct caaatcgttg cttacagata ctattattcg ttttggtttc
     1141 ataatatttt tcaaaactac aaaatatatt tatagatttt atattttgta aataatttct
     1201 aaatttgtta caaaattatt taacgatgca gatatttaaa aattgttcaa ttaaaaagta
     1261 caatactttt gggatagatg taaaagcctc tgttttagtt gagtacgatc acgatgatga
     1321 aattattgat tttctaaaat ctaatcaaga taaaatactt aaacttttgg ttgttggtgg
     1381 cggaagtaat ttgctatttc aaaatgattt tgatggttta ataattcatc ctgttgggca
     1441 aagaatggag gtgattaaag aagatgaaaa ctttattttt ataaaagctt cagctggata
     1501 tgtgtgggat aagtttgttg aagatacttt aaatagcaag gcttatggtt tagaaaatct
     1561 ttctttgata ccaggaactg taggagcgtc tgtagttcaa aatattggag cttatggggt
     1621 agaagcaaaa gattttgttg atgaagtaga atgtctagat ttgaattctt acgaaattaa
     1681 aacaataaaa aaatttgact gtaaatttgc atatagaaat agcattttta aaaatcaact
     1741 aaaagataaa gttttggttt taaatgttat ttacaaacta tttaaacaac ctaaattaaa
     1801 ctgcgaatac actgatattc agaaatattt taatgggaaa actgatgtta gtgcaaaaga
     1861 tgttagggaa gctgttataa atattagaaa ttttaaattg cctgacccag aacaaattgg
     1921 aaatgcagga agttttttta aaaatccaat tattaatcaa gaaaagtttg atattttaat
     1981 aaaaaaatac gaagacttac ggttttacaa cttgccaaat gggaattaca aacttgctgc
     2041 tgcttggatg attgataaat taggttttaa aggctttaaa cacaaaggag ctgctgtgca
     2101 taaaaatcaa gctttggtaa taataaatga aactggcgaa gcaactggca gtgatatttg
     2161 cgaactagca gaaataatta aagaaaaagt atttcaaaat ttttatgttt tattagaacc
     2221 agaagttatt tatgtttaat agtctgattt ataataaatt gcatacaata taaaatcttt
     2281 tgaaaaaata attaaaaaaa atttggtaga aagaaaaaaa atgttttatc tttgcaccgc
     2341 atttgcgaga atagctcagt tggtagagca cgaccttgcc aaggtcgggg tcgcgagttc
     2401 gagtctcgtt tctcgctctt tttttttaaa aagggcatat atgtgtgccc ggatggcgga
     2461 ataggtagac gcgctggact taaaatccag tgatttttaa aaatcgtgcc ggttcgattc
     2521 cggctccggg tactaaggaa agacagagtt gaaaaattct gttttttttt gttctaaaca
     2581 tttttaatag atttctgttg taatacagat ttaatacaat tttagtgtaa ttttagtaaa
     2641 atgtataata aacgaataaa aattataata atgaaaaaaa ctttaatttt aactttgatt
     2701 ttaagctttg cttgtataaa tgtgttttca caagaggaag cagaacaaaa aaaaggagaa
     2761 tggaaaaaag gtggaatgat gtctttaaat gcttctcaag tttcttttac taactgggct
     2821 gctgggggac aaaactctat tgcagcaaac tcatttgtga atttatttgc taattttaag
     2881 aaaaatcgaa tgacttggga caatactttg gatttaggtt atggtaaact gtttcaaggg
     2941 ataaaagaac ataaaacagt ttataaaaca gatgataaaa tagatttttc ttcaaaatac
     3001 ggacgttatg ctttcgaaca ctggtattat gctgctttga taggctttaa aactcaattt
     3061 gaccaaggtt ttaaaaatcc aacagataca gttaaaatat cagacttttt ctctccagct
     3121 tatctaaact tatctatagg tatggactat aaaccatgtg ataatttttc gctattaatt
     3181 tctccattaa caggtaaagt aacttttgtt attgacgagt atttatctag cataggagct
     3241 tttggtgttg acccaggaaa aacttttaga cctgagtttg ggggatatat taaagctcaa
     3301 tataaagtta attttttaaa aaatatgact tattcaacaa aaatcgacct tttctctaat
     3361 tacttggaaa aaccacaaaa tattgacgtt aactgggaaa atcttgtaat gatgaaaatt
     3421 aataattggt ttagtgcaag cattcactat acaatgattt atgatgatga tgttaatgtt
     3481 aaatggattg atgcagataa tatagaacat tttagtccaa aacttcaaat taaacaaatg
     3541 ataggtgttg gacttacata taaattttaa tttaaaaaaa ctacaattat gttaaaagaa
     3601 tttagagatt ttgcaattaa gggcaacctt atagacatgg ctataggtat tataattggt
     3661 gctgcttttg gaaaagttgt agcatcatta gttgccgatg ttattatgcc acctatagga
     3721 ttattaatcg gtggtgttaa ttttaccgat ttgactatag ttttaaaaga agcagttggt
     3781 gataatcctg ctgtaacttt aaattatgga aattttattc aaatccttat tgatttcttg
     3841 attgttgcaa tggcaatatt tattgttatt aaaggcatta acgctttgaa aagaaaagaa
     3901 gaaaaagaac cagaaccaga gccagaacca gaaccaacaa aagaagaaat tcttcttaca
     3961 gaaattagag atttattgaa agaaggcaga aaagaataaa acctttttac ttgagcttgt
     4021 tttaaaacaa attttagaac aaaaataata aaggtgtttt acatatgtaa ggcatctttt
     4081 tttctgaaaa ataaaaacat aaaatatctt tcagttttta agattaacat attatttttg
     4141 tcaaaaatat tgaaaaatgt ttaattattt taattatatt atatgggacg ttggtcctat
     4201 tcttgttgat cttggaatat tgcaaataag agcttatagc ttgttttttg ctttgggatt
     4261 tgttgttagc tattttatta taaaaagata ttacaagcaa gaaggagtac ctattcgcga
     4321 attagacact cttacagtat gggttgtggt tggtggcttg ttgggtgcca gattaggaca
     4381 ttgcattttt tatgattggg aatattattc taaaaattta ttggaaattc ttttgcctgt
     4441 aagtttttct ccaaaatttg aatttattgg ctatcaggga cttgcaagtc atggtggagc
     4501 aataggaatt ataattgcaa tattgattta ttatagaaaa tctacaaaaa aatctttctt
     4561 ttggcttatt gatagaatag ctgtgccaac aggttttgca ggtgctttaa tacgcttggg
     4621 aaatttattc aattccgaaa tttatggtca tcaaacagat ttgccatggg gatttgtttt
     4681 tatacgcaac ggagatacat ttccaagcca tccaacacag ttgtatgaag caatttgcta
     4741 tattattacc tctatagttt tgatgagatt gtataaagtt gaaagattta gaaatgctgg
     4801 ggggttttta cttggagttt tctttatcat ggtgtttact gcaagatttt ttatagaatt
     4861 tgtgaaggaa aatcaagtgg catttgaaga ttctatggca ttaaatatgg gacaaatttt
     4921 aagtattcct gttgttgtgg ctggaatatt tttagttgta agagctttga aactaagtaa
     4981 agcaaaataa aatatataat atgttaaagc aaatttcatt actattttta ctcttgttgc
     5041 cttggttaag tttttctcaa acttataaaa ttacttataa aagctattct aacggaaaat
     5101 atcaagaaga cggagatttt tcaatttatt ttaaagaaaa tattgtttat ttgtcttcgc
     5161 aaaatgctaa agttcagcag tttacagatt ttaataaaaa agaaaactct aaaataatgg
     5221 agtttgaaaa taatttatat cgtaattcaa cagctttttc agaactttta aagccagaaa
     5281 acattgaaaa ttctactgag acaattcttg gatataaatg taaattagct gtgttttatt
     5341 cattttcaaa caaaatagaa gtttggttta ccgacaaagc accagcttat ggaaatcctt
     5401 attctgcctt tattcctgaa gaaaaatctt tagctttaaa agttgttgta aatggaaata
     5461 gagaaattat agcgtcagaa attcaaaaag taaaaaatat agaattgcct gaatatcaat
     5521 atgataaagc tgtaaaaatc tcggattctg aatttgaaga aaaagtgatt ttatcgcgtt
     5581 attctcgttt aagtattttt gaaaatgcac aaataaattt tgatacggat attgagcaaa
     5641 ttaatcttga aaacactgaa ccagatgtgg tttatcattt gtctaatggt ggaattgtct
     5701 tgaaaaaagt aagaatagat gaatctttta aaaattcgta tttttatgtt gaattagatg
     5761 tgaaatcaaa tggagatgct tacgacagaa caggttcggt ttttatgttg ccagacaatg
     5821 atgattttcg tccaatgtta aaagctatat ttggcgataa aagcgaaatg cctgtatttg
     5881 aagataaata tggggagaaa tatcaaggaa tgattgcaac ttcagagttt ttaccacaaa
     5941 ttgagttaat gcgttttttt acatcttttg gagctggaca ttttaatact ttgcgtccaa
     6001 taaataatta tccttgggct gaaaaaatta cctataaaca agatgtttca caattattgc
     6061 ctgatgatga atatgtttgg tttggcgtat ttatcggaaa ttacgataag ggcggacata
     6121 ttataaattt aaaacttagt ttttatccaa gttttggttc tgctgatgaa aatgtgcaaa
     6181 aatttattct tccagttttt aatacggtaa atattttaga aatgtcaggg caaaattacg
     6241 gtaagttttt caagtcagat actttagtaa gtgagtttac agttcccgaa aatttagaaa
     6301 atcctgtttt gctatatact tctacaggtc atggaggctg gggtagtgga gatgagtttg
     6361 tgccaaaaat gaataaaatt tttattgatg ataaagaaat ttatagtttt attccttggc
     6421 gttatgattg tgcaacttac agattttcta atccagcttc tggaaatttt agtaatggtt
     6481 tgtcgagcag tgatttaagt cgctctaatt ggtgtcctgg aactt
//
LOCUS       DAOPNX010000115         6390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_13312_length_6390_cov_8.052723, whole genome shotgun sequence.
ACCESSION   DAOPNX010000115 DAOPNX010000000
VERSION     DAOPNX010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6390
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13312_length_6390_cov_8.052723"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PKN53_10020"
     CDS             complement(<1..839)
                     /locus_tag="PKN53_10020"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89324.1"
                     /translation="MKFFNSLLTKIIIFILYIALFVLNSCNQNYTKKKLELNEGWEFY
                     FERTSKWYPATVPGNIHTDLFANNLIPDPFAENNADSLAWVSNQIWKYRKSFDISPDF
                     INRNLELVFEGLDTHAEVFLNDTKLGNANNMFRPWIFKIDKSLRKEKNNLIEVIFYPS
                     SEYNKLKEDEGAYSIPDNHGNTRKSPYQYGWDWAPNLETCGIYKDVYLNTWEKMRINN
                     VNIEQKTLNDTIAILQANVKLESEKFYNGKITISSPNNEFDSIIQRIDVNEGINLYQI
                     EFE"
     gene            841..2457
                     /locus_tag="PKN53_10025"
     CDS             841..2457
                     /locus_tag="PKN53_10025"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOD89325.1"
                     /translation="MQKFIFLSIFVLVFTLSCKDKKNYSFENNLIPKPLELNFNNGEL
                     EVKDNIYLVFTDNSVDAQRVQNFVKNFLGSVYNVSVVSEKQKDGTNIFFKIAGEGDNE
                     SYTLNIDKNGVELVSPAYNGLFLGFQTLRQLFPAELEAGEKPETVNLPYVSIKDSPRF
                     KYRGMHMDVCRHFFGVENVKTYIDMLVMHKFNTLHMHLTDDQGWRIEIKAFPNLSTIS
                     STRRGTVIKQNWGEYDEIPVSGMFTQDQIREIVKYAEDRFITIIPEIEMPGHALAALA
                     AYPELGCTGGPYEVSRTWGVFDDVFCAGNEKTFEFFEKVIDEVVELFPNSPYIHVGGD
                     ECPKVRWKECPKCQQRIKDENLANEEELQSYFITRMEKYINSKGKRIIGWDEILEGGL
                     APDATVMSWRGTEGAVAAAKLGHDAILCPGSHCYFDHYQSEDKENEPLAICCYTSTEK
                     VYSWEPIPDSIPVEQHKHFLGVQANLWAEYIKEFSHLQYMAFPRMTALSEVAWCTERD
                     PDYAQFKKRLQVMFKRYDKAGYNYAKHELE"
     gene            2469..3092
                     /locus_tag="PKN53_10030"
     CDS             2469..3092
                     /locus_tag="PKN53_10030"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOD89326.1"
                     /translation="MGNIELFNKLKTLMQNPLPGMCAQEKMIPNFPDNTPEYFNYDKN
                     LRDAAVLIVLFEEDTRLKTFFIERMPEPGPHSGQIAFPGGRVEKIDKDLFDTAIREAF
                     EETGVKVKRENILGSLTPVEIPISGFSVLPVVAFYDTIPKFIPCEKEVKSVFTVDLFN
                     LFESESIKNVSTRNIEVQAPCFISDDKIIWGATAMVLKEFKEMFKCS"
     gene            complement(3565..4197)
                     /locus_tag="PKN53_10035"
     CDS             complement(3565..4197)
                     /locus_tag="PKN53_10035"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MjaI family restriction endonuclease"
                     /protein_id="HOD89327.1"
                     /translation="MAKEWILNSAMNRFQLNFKRNVGPTSESIRQCEPKTLEEWREYY
                     FSNVRSKDHIIDLGKKLYIKITEVISAEVEEITEQDCIDYMLQLVIERTFDGYWTEIK
                     TIYGQLEQELGYKIEPAPDKWDRLFNVDFFIKIKERFIGLQIKPVNQGIQLSQIFKEK
                     GLQQKTHEKFENEFGGKVFYIFSSKANGKKVIMNPEVIEEIRTEIHRLEQ"
     gene            complement(4199..4852)
                     /locus_tag="PKN53_10040"
     CDS             complement(4199..4852)
                     /locus_tag="PKN53_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89328.1"
                     /translation="MANNYWLEQEKKFLKAIENVDVPNEKLDNVDFIDLKSHQDLGNI
                     GVGGGCYWIWTNEPVIHSLHKNPIPKPFDNGELIYNGIAKDDVKGRVFHHLFGFEDAG
                     WSGISIDIYTKASNSHRKKACSPNGKVPFINNSPIRTKEQLLKLHLTQEEKEFINNSE
                     QSVFHFRNGINLNDEKHKDFKFRVYFITGLTSLYLEHIEKEWRKNGLPKLCSYLTGR"
     gene            complement(4842..6092)
                     /locus_tag="PKN53_10045"
     CDS             complement(4842..6092)
                     /locus_tag="PKN53_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOD89329.1"
                     /translation="MNLVPDKSVHLVITSPPYWQLKDYGTENQIGYHEDYETYINNLN
                     LVWKECYRVLDNGCRLCVNIGDQFARAVYYGRYKVIPIRTEIIKFCESIGFDYMGAII
                     WQKQTTTNTTGGASLMGSFPTPRNGILSIDYEFILIFKKLGTSKSKVSKEIKEQSKMT
                     TEEWKEYFAGHWNFGGARQDGHIAMFPEELPKRLIKMFAFKGETVLDPFMGSGTTSLA
                     ARNLERNSIGYEINPEFIEIAKDKLNIRQSDITGTTYEFLKDEININIEDEFENLPYR
                     FVDFQNLDKKVDPKKLQFGSKIDKNSGQREDYYTVKEVISPELVKLNNDLIVRLIGVK
                     EKKSANGSAKQFLYDKTKGQKVFMKFDNQKYDDQNNLMCYLYLKNKTFLNAHLIKEGL
                     VEPDTEADFKYKNKFLSLNTSNGK"
BASE COUNT         1996 a         1054 c         1005 g         2335 t
ORIGIN      
        1 atttcaaatt ctatttgata taaatttatt ccttcgttta catctattct ttgtataatg
       61 ctgtcaaatt cattgtttgg tgaagaaatt gttattttcc cattataaaa tttttctgat
      121 tctaatttta catttgcttg gagaattgcg attgtgtcgt ttaaagtttt ttgttcaata
      181 ttcacattat taattcgcat tttttcccaa gtattcaaat atacatcttt ataaattccg
      241 caagtttcaa gatttggtgc ccaatcccaa ccatattgat atggtgattt tcttgtattt
      301 ccatgattgt cgggaataga ataagcgcct tcatcttctt tcaacttatt atattcggaa
      361 gatggataaa aaattacttc tattaaatta tttttttctt tccttaaaga tttatcaatt
      421 ttaaaaatcc aaggtctaaa catattgtta gcatttccaa gttttgtatc attcagaaaa
      481 acttcggcat gtgtgtctaa tccttcaaaa acaagttcta aatttctatt aataaaatca
      541 ggacttatgt caaaactctt tctatatttc caaatttgat tgctcaccca agctaaacta
      601 tctgcattat tttctgcaaa agggtctgga ataagattat tggcaaataa atctgtgtga
      661 atatttccag gtactgtagc aggataccat ttgcttgttc tttcaaaata aaactcccac
      721 ccttcattaa gttctagttt tttcttagta taattttggt tacaagaatt taacacgaat
      781 aaggctatat ataaaataaa aattattatt tttgtcagca aactattaaa aaacttcatt
      841 atgcaaaaat ttatttttct ttcaatattt gttttagtgt tcacactatc gtgcaaagat
      901 aaaaaaaact attcgtttga aaacaattta attccaaaac ctttagaatt aaatttcaac
      961 aatggagagc ttgaagttaa agacaatatt tatcttgttt ttaccgacaa tagtgttgat
     1021 gcacaaagag ttcaaaattt tgtaaaaaac tttttaggaa gcgtttataa tgttagtgtt
     1081 gtttctgaaa agcaaaaaga tggaacaaat attttcttta aaattgctgg agaaggcgat
     1141 aacgaatcat atactttaaa tattgacaaa aacggtgtag aattagtttc tcctgcttat
     1201 aatggtttgt ttttaggatt tcagacttta cgtcaattat ttccagcaga gcttgaagct
     1261 ggtgaaaaac ctgaaacagt aaacttgcct tatgttagca taaaagacag tccacgcttt
     1321 aaatacagag gaatgcacat ggacgtatgt cgtcattttt ttggtgttga aaatgtaaaa
     1381 acttatatcg atatgttggt aatgcataaa tttaacactt tgcacatgca tttaaccgac
     1441 gaccaaggtt ggagaattga aattaaagca tttccaaacc tatcaacaat ctcttcaact
     1501 cgcagaggaa ctgtaataaa acaaaattgg ggcgaatacg acgaaattcc tgtaagtgga
     1561 atgtttactc aagaccaaat tcgcgaaatt gttaaatatg ccgaagatag atttattact
     1621 attattccag aaattgaaat gcctggtcat gctcttgctg cgttggctgc ttacccagaa
     1681 ttaggttgca ctggtggtcc ttacgaagtt agcagaactt ggggagtttt tgatgatgta
     1741 ttttgtgcag gtaacgaaaa aacttttgaa ttttttgaaa aagtaattga tgaagttgtt
     1801 gagttgtttc ctaactctcc atatattcat gttggcggag atgaatgtcc taaagtaaga
     1861 tggaaagaat gtccaaaatg tcaacaaaga ataaaagacg aaaatcttgc taacgaagaa
     1921 gaattacaaa gctattttat tacaagaatg gaaaaataca taaacagcaa aggcaaacgc
     1981 attataggtt gggacgaaat tttagaaggt ggcttagctc cagatgcaac agttatgtcg
     2041 tggcgtggaa ctgaaggagc tgttgctgct gcaaaacttg gacacgatgc tattttatgt
     2101 cctggaagcc attgttattt cgaccattat caaagcgaag ataaagaaaa cgaaccttta
     2161 gcaatttgtt gctacacaag tacagaaaaa gtttattcat gggaaccaat tccagatagt
     2221 attcctgtag aacaacataa acattttctt ggtgttcaag caaatttatg ggctgaatat
     2281 attaaggaat tttctcactt acaatatatg gctttccctc gtatgactgc tttaagcgaa
     2341 gttgcatggt gtacagaacg cgacccagat tatgcacaat tcaaaaaacg cttacaagta
     2401 atgtttaaaa gatacgataa agctggctat aactatgcaa aacacgaact agaataatta
     2461 ttaaaactat gggaaatatt gaattattta acaaattaaa aactttgatg caaaatcctt
     2521 taccgggaat gtgcgctcaa gaaaaaatga ttcctaattt tcctgataat actccagaat
     2581 attttaatta cgataaaaat ttgagagatg cggcagtttt aattgttttg tttgaagaag
     2641 atacaagatt aaaaacattt tttatagaac gcatgcccga accaggacca cattctggtc
     2701 agattgcttt tccgggtgga cgtgttgaaa aaatagataa agatttattc gacactgcta
     2761 tacgcgaagc ttttgaagaa acaggcgtga aagtaaaacg agaaaatata ttaggcagtc
     2821 tcactcctgt tgaaattcct ataagcggtt tttctgttct tcctgtagtg gctttttacg
     2881 acacaatccc aaaatttatt ccttgcgaaa aagaagtgaa gtctgttttt actgttgatt
     2941 tgtttaattt gtttgaaagt gaaagcataa aaaatgtttc tacaagaaat atcgaagttc
     3001 aagctccatg ttttattagt gatgataaaa taatttgggg ggctacagct atggttctaa
     3061 aagagtttaa agagatgttt aaatgtagtt gatattcttt tattttttta agttgatttt
     3121 atacttcaca cctcccatta tccaaaatcc gggctgtgtt aaacctccga tatcaaaata
     3181 tggaacatta aatacgttat tgatatttaa gtgaaaatcc caatttctat atttatattg
     3241 aattaagtgt ccccttttat taggacagtt tttttaattt gttaatattt tatttttcaa
     3301 agaacttaaa tctttaactt tgcaaagtta aagatttttt tgaaactttc aagtgtttta
     3361 cacaattttt ttaaataatt tttcaggtgt ttgataatca agtgattggt gtttcctttc
     3421 gcaattataa cgttttgcgt gtatgtgcag tagcggatta gaagcacttt cctgtccgtt
     3481 tagcacaaag tttcttagaa atatagacct tcgatttacc acttcacccg ctattgccac
     3541 atacacgctg ttgggtgctg ggctttattg ttcaagtcgg tgaatttcag ttctgatttc
     3601 ttcaataact tcaggattca ttatcacctt tttcccgttt gctttggatg agaaaatgta
     3661 aaataccttt ccaccgaatt cattctcaaa tttttcgtgt gtcttttgtt gtaaaccttt
     3721 ttctttgaaa atttgagata attggattcc ttgattaaca ggctttatct gcaatccaat
     3781 aaatctttct ttaattttga tgaagaagtc aacattaaaa agtctgtccc acttgtctgg
     3841 tgccggctca attttgtaac caagttcctg ctcaagttga ccataaattg tttttatttc
     3901 agtccaatag ccatcaaagg ttctttcgat gacaagttga agcatatagt caatgcagtc
     3961 ttgttcggtg atttcttcaa cttctgctga aatgacttct gttatcttga tgtagagttt
     4021 tttaccaagg tcaattatgt ggtctttgga acgcacattt gagaaatagt attctctcca
     4081 ctcttccaaa gtttttggtt cacattgtct gatactttct gatgttggac caacatttct
     4141 tttgaagttc agttggaagc gattcatcgc actatttaat atccattctt tagccatatt
     4201 atctaccggt taaataggaa caaagtttag gtaatccatt tttacgccat tctttttcaa
     4261 tgtgttcaag ataaaggctt gtaagacctg ttatgaagta aaccctaaac ttgaaatcct
     4321 tatgtttctc gtcatttaag tttattccat ttctaaaatg aaagacgctt tgctcgctgt
     4381 tgttaataaa ttctttttct tcttgggtaa ggtgcaattt caaaagttgt tccttagttc
     4441 ttattgggga attattgatg aaaggtactt tgccatttgg tgaacaagcc ttttttctat
     4501 gactattaga tgctttcgtg tatatatcaa tacttatacc cgaccaaccc gcatcttcaa
     4561 atccgaaaag atgatgaaaa acccttcctt ttacatcgtc tttagctata ccattgtata
     4621 ttaattcacc attatcaaaa ggttttggga ttgggttctt atgcaaagaa tgaataactg
     4681 gctcattagt ccatatccaa taacatccac caccaacgcc aatatttccc aaatcttgat
     4741 gtgatttcaa atcaatgaaa tccacattgt ctaatttttc gtttggcaca tcaacatttt
     4801 cgatggcttt tagaaatttt ttctcttgct caagccaata attatttgcc atttgatgtg
     4861 tttaaactta aaaacttgtt tttgtattta aaatctgctt ccgtgtcagg ttcgactaac
     4921 ccttccttaa tgaggtgagc attcaaaaag gttttattct ttaaatacaa gtagcacatt
     4981 aaattattct ggtcatcgta tttttgatta tcaaatttca taaatacttt ttgtcctttt
     5041 gtcttgtcgt ataaaaactg cttggcagaa ccattggcac ttttcttttc tttaacgcca
     5101 attaatctga caataaggtc attattaagt tttaccaact ctggactaat aacttctttt
     5161 accgtatagt aatcttctcg ctgcccacta tttttatcga tttttgaacc aaattgaagt
     5221 ttctttgggt caaccttctt atcaaggttt tggaaatcaa caaagcgata aggtaagttc
     5281 tcaaactcat cttctatgtt gatgttgatt tcgtccttta gaaactcata agttgttcct
     5341 gtaatatcag attgcctaat atttaatttg tcctttgcaa tttcaatgaa ttcaggattt
     5401 atttcatatc caattgaatt cctttctaaa tttcttgcag ccaaagaggt tgttccacta
     5461 cccataaatg ggtctaaaac ggtttcgcct ttaaaagcaa acatttttat tagtctctta
     5521 ggtaactctt ctggaaacat tgcgatatgt ccatcttgtc ttgctccacc aaaattccaa
     5581 tgacctgcga aatattcttt ccattcttcg gtagtcattt tagattgttc ttttatttct
     5641 ttgcttacct ttgatttgga agtgcctaat tttttaaaaa ttaaaataaa ttcatagtca
     5701 atagagagaa ttccatttct tggtgtcgga aaacttccca ttaatgatgc tccacctgtt
     5761 gtatttgttg tggtttgctt ttgccaaata atagccccca tataatcaaa cccaatgctt
     5821 tcacagaatt tgattatttc agttcggatt ggaataactt tataacgtcc ataataaaca
     5881 gctcttgcaa attggtcgcc tatatttaca catagtctgc aaccgttgtc aagaacccga
     5941 taacattcct tccaaacaag attgaggttg ttaatatagg tttcataatc ttcgtgatag
     6001 cctatttgat tttctgttcc atagtccttt agctgccaat aaggtggcga ggttataact
     6061 aaatgaaccg acttgtcagg cacaagattc atctgtcggc tgtctccgtt tattatttta
     6121 tgtgtcgtct tcactctaaa tttcttttta attgatgttg tctgcaaatg tagtaataat
     6181 ataaggtttt ctgttcgatt tctggtttct tagccttgca cccaacatct aatttacgca
     6241 acaaatatac aacattcgta tatattaaac ttataacatt cgtatattat tttttgattt
     6301 aattcaaata aatattatat aatccaatat atcatataaa taactaaaat aaaaagaagt
     6361 aaaaatattt gtatttacga atgttttgtt
//
LOCUS       DAOPNX010000116         6380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_13344_length_6380_cov_6.120949, whole genome shotgun sequence.
ACCESSION   DAOPNX010000116 DAOPNX010000000
VERSION     DAOPNX010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13344_length_6380_cov_6.120949"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            317..1543
                     /locus_tag="PKN53_10050"
     CDS             317..1543
                     /locus_tag="PKN53_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOD89330.1"
                     /translation="MIKKQKIINDPVYGFINIPGGLIFKLIEHPYFQRLRRIKQLGLT
                     YLVYPGAAHTRFQHSIGSMHLVNLAIDVIRSKGHKITEDEAEATRIAILLHDIGHGPF
                     SHALENCIIPDLHHEKLSLWFMDELNKEFNSELSLAISIFKNQYHKKFLHQLVAGQLD
                     MDRLDYLRRDSFFTGVTEGVVGSQRIIKMLQVVDDNLVVEAKGIYSIEKFLIARRLMY
                     WQVYLHKTVLVGELLLSHFLKRLKYLYKEKGIKTGNVYLDFLFEDNNMSDKELIIKNF
                     SMLDDYDIMSLIKQNINSQDRVIAGLASSLINRKLPRIRLSKEEIDYDFLNKIKQQFM
                     KKYNFSAEETDYFVYSGEISNNAYDREDERINILFGEKLVDITEASDILNISVLGKIV
                     KKNYICAPKYIMNKIS"
     gene            1545..2573
                     /gene="lpxD"
                     /locus_tag="PKN53_10055"
     CDS             1545..2573
                     /gene="lpxD"
                     /locus_tag="PKN53_10055"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOD89331.1"
                     /translation="MEFTAQQIAELLNGKVEGNPNAVVSTFSKIEEGKPGSLCFLANP
                     KYTNYIYETQASVVLVNEDFEAQKPIKATLIRVEDAYRAIAILLDIYNKMTDKPKGIE
                     KQSFVHKSAVLGKNIYVGAFAYIGKDVKIGNNVQIYPQVYIGNNVTIGDNCIIYPGVK
                     IYYNTKIGNNCIIHSGTVIGADGFGFAPNDGEAFLKIAQIGNVVIEDNVELGALVTVD
                     RATMGSTIIREGVKLDNHNHVAHNVEIGKNTVIAAQTGIAGTSKIGQNCMFGGQVGIG
                     PHITIANGTKLSSKSGLSNNVLKEDSLLMGAPAFDIRAYHRAYIHFKNLDDIVKRIDS
                     IENKLNEK"
     gene            2596..4482
                     /locus_tag="PKN53_10060"
     CDS             2596..4482
                     /locus_tag="PKN53_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOD89332.1"
                     /translation="MKKLNINILRRITPRWVVFLIDVSICLVSLILAYYLRFNFIIPS
                     KERDSLAMVVVLVLSVRMLSFYIGKTYAGMVRYTGTKDIIRIFIVLFIGSAVLMVTNL
                     IYLELYDKYLIPTSIIIIDFFITTFILSFSRLLLKNIYLEFNSPRRESDNVLIYGSGE
                     LALITKKTVDNDSKSDYRVVGFIDHHNKLKGSKIDGVSVYHLKDLKKILDEKKVSVVI
                     ISEKGMPISRKNYVIDTCLDRDVEVKTIPYLTSLLSDSFSLSQLKNIRIEDLLERPEI
                     KMDKDGIRKNHLNKTVLITGAAGSIGSELVRQIIRFLPSKVLLLDIAESPLYDIELEI
                     RENFKFNNIEIIIGDVRNKDLINYIFEKYKPDFVYHAAAYKHVPMMENHPNEAVKTNV
                     KGTKVVAEAAIKYKTRKFVMVSTDKAVNPTNVMGASKRIAEIVVQSLSKTSETQFVTT
                     RFGNVLGSNGSVIPRFRKQIEAGGPVTVTDPEITRYFMTIPEACQLVLEAGNMGEGGE
                     VFIFDMGKPVKIINLAKKMISLSGLKPGKDIQISITGLRPGEKLYEELLANKENTLPT
                     YHPQIMIAKLKEYDADEVLPEINMLIENAIEKNDNFELVKKMKEIVPEFISKNSVYEV
                     LDTN"
     gene            complement(4496..5428)
                     /locus_tag="PKN53_10065"
     CDS             complement(4496..5428)
                     /locus_tag="PKN53_10065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOD89333.1"
                     /translation="MNKTYENKYFFLINPNAGNSEGEREWTNVLEVINKNNLDFDFAF
                     TEYIGHAEEIVSKQIKIGLRNFIILGGDGTLNEVTNAVLKQNEVNSQEISLGIIQMGT
                     GNDWSKYYKFDNDYQKAIDRFKSKNTKFQDVGVIKYKADNEDKKAYFINIAGFCFDSV
                     VVKATNQMKERGKRTNLAYMINLLKSLITYKPWELKIHINDEILEGKFLSISIGNGKF
                     SGGGMIQTPDAIIDDGWLNVTIYKNMSKFKIVANIRRLYNGNIGKVKGVRMFKTKKFF
                     IEADQDIFVETDGEIIDYAPYEISIIPQAIKVFV"
     gene            5488..6330
                     /locus_tag="PKN53_10070"
     CDS             5488..6330
                     /locus_tag="PKN53_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89334.1"
                     /translation="MAKKIRLIKSYQSEINKPETEYLAVVKEYNEKGMEIRLVEYTED
                     NEIEYEHTLECDSDFNPIREEIISKSDEYSEQKTFKYDENNKLIEEKIQYQNAWESIK
                     KYERDQEKRCVIITTFDEEGEVEESSEIYYNEKGEIITRIENDEYGKQKNKVKNSFDE
                     KGILISKEEYSNTKKPDKIYHYHYNENGDLYAVETQNSRNRLIDWTKIVFDDKNRPIE
                     QVNMAGTKLVIEYNDEESEITETYYLANGTIYNKTKYKKNSEGLTEYEETSDKITRYV
                     YEYF"
BASE COUNT         2401 a          803 c         1050 g         2126 t
ORIGIN      
        1 tgaaacacct tgaaaattca ttgttttaaa atttgttttg agacagcctc tttaaaaata
       61 gtaatgataa agtacgaaaa ataaattgta caagataaaa ttttcaaatc tgattttgac
      121 cgtgaaatga tgaaattttt aaatgaaaat aacgaataat cagcagtatt ctgttttcaa
      181 acaataaaat agttgactta ataagagaaa tagcaagtgt ggaataattc tttgttacct
      241 attttaatat taatttgcac attactattt tattttaaca atatttataa tacttttgta
      301 taattaaatt ttgctagtga ttaagaaaca gaaaataata aacgaccctg tttatggttt
      361 tataaatatt ccgggcggtt taatttttaa acttatagaa catccttact ttcagaggtt
      421 gaggcggata aagcaattgg gcttaactta tttagtttat ccaggtgctg ctcatactcg
      481 ttttcaacat tctataggct ctatgcattt agtaaattta gcaatagatg taattagaag
      541 taagggacac aaaatcactg aagacgaagc agaagcaact cgcatagcta ttttacttca
      601 cgatataggt catggacctt tttctcatgc tttagaaaat tgtattattc cagatttgca
      661 ccacgaaaaa ttaagtttgt ggtttatgga cgaattaaat aaagaattta attctgaact
      721 tagtttagca atttcaatat ttaaaaatca atatcataaa aagtttttgc accaacttgt
      781 tgctggtcaa ttagatatgg acaggttgga ctatttgcga agagatagtt tttttacagg
      841 agtaacagaa ggagtggtag gttcgcaaag aattattaaa atgctacaag tagttgacga
      901 taatttggtt gttgaagcaa aaggaattta ttctatagaa aaatttttaa tagctcgccg
      961 actaatgtat tggcaagttt atttacataa aactgtgctt gtaggcgagt tgcttttatc
     1021 gcacttttta aaaagattaa aatatttata taaagaaaaa ggaataaaaa caggaaatgt
     1081 ttatttagat tttttgtttg aagacaataa tatgtctgat aaagagttga taataaaaaa
     1141 cttctcaatg ctagacgatt atgatattat gagtttgata aagcaaaaca taaactctca
     1201 agatagagtt attgcaggat tagcaagctc attaataaat cgaaaactac caagaataag
     1261 attgtctaaa gaagaaatag actatgattt tttaaacaaa ataaaacaac agtttatgaa
     1321 aaaatataat ttttctgccg aagaaactga ttattttgtt tattctggag aaatatcaaa
     1381 caacgcttac gatagagaag atgaaaggat aaatatttta tttggagaaa aacttgttga
     1441 tattacagaa gcttcagata ttttgaatat ttcagtttta ggaaaaattg taaagaaaaa
     1501 ttatatttgt gcaccaaaat atattatgaa caaaataagt taatatggaa tttacagctc
     1561 aacaaattgc agaattattg aatggaaaag tagaaggcaa cccaaatgcg gttgtaagta
     1621 ctttttcaaa aattgaagaa ggaaaaccag gctctttatg ctttttggca aatcctaaat
     1681 atactaatta tatctacgaa actcaagcaa gtgttgtgtt agttaacgag gactttgaag
     1741 ctcaaaagcc aataaaagca accttaattc gtgttgaaga tgcttataga gccatagcaa
     1801 tattgcttga tatttataat aaaatgacag acaagcctaa aggaatagaa aagcaaagtt
     1861 ttgttcataa atctgcagta cttggaaaaa atatttatgt aggagcattt gcttatatag
     1921 gtaaagatgt gaaaattggt aataatgttc agatttatcc tcaagtttat ataggcaata
     1981 atgtaactat cggagataat tgtattattt atccaggtgt taaaatttat tataatacaa
     2041 aaattggaaa taactgtata atccattctg gtactgttat tggagcagat ggctttggtt
     2101 ttgctcctaa tgatggagaa gcatttttga aaattgcaca aattggaaat gttgttatcg
     2161 aagacaatgt agaacttgga gccttagtaa ctgttgatag agcaactatg ggttctacaa
     2221 tcattcgaga aggtgtaaaa ttagataatc acaaccacgt tgctcataat gtagaaatag
     2281 ggaaaaatac agttatagca gcacaaacag gaattgctgg aactagcaaa ataggtcaaa
     2341 actgtatgtt cggcggacaa gtgggaatag gacctcatat tacaatcgca aacgggacaa
     2401 aactttcgtc taaatctggg ctatcgaata atgttttaaa agaagatagc cttcttatgg
     2461 gagctccagc atttgatatt agagcatatc atagagctta tatacacttt aaaaacttag
     2521 atgatatagt gaagagaatt gacagtattg aaaataaact taatgaaaag taaaattatt
     2581 tgctaatttt gtactgtgaa gaaattgaac attaacatat taaggagaat tacacctcgt
     2641 tgggtagttt ttttaattga tgtaagcatt tgtttagtct cgttgatact agcttattat
     2701 ctaagattta actttattat tcctagcaag gaaagggata gccttgcaat ggttgtagtc
     2761 ttagttttga gtgttagaat gctttcattt tacattggta agacatatgc aggaatggta
     2821 agatataccg gtaccaaaga tattatccga atttttattg ttttgtttat cggttcagct
     2881 gtattgatgg ttactaatct tatttacctc gaattgtacg acaaatattt aattccaact
     2941 tccataataa taatagactt ttttataacc acttttattc tttctttttc tcgcctattg
     3001 ttaaaaaaca tataccttga atttaatagt cctcgtagag aaagtgataa tgtgcttatt
     3061 tatggctctg gcgaattagc tttaattact aaaaaaactg tggacaatga tagtaaatca
     3121 gattatagag tagtgggatt tattgatcat cataataagt tgaaaggaag taaaattgac
     3181 ggcgtaagtg tttatcattt aaaagatttg aaaaaaattt tagatgagaa aaaagtttct
     3241 gttgttataa tttcagaaaa aggaatgcca attagtcgta aaaattatgt tatagacact
     3301 tgcttagata gagatgttga agtaaaaacc ataccttatt taacatcatt gctgagcgat
     3361 agctttagtt tgagccaatt aaaaaatatt cgcatcgagg atttattaga gcgtcccgaa
     3421 attaaaatgg acaaagatgg aataagaaaa aatcatttga ataaaactgt tttaattact
     3481 ggtgctgctg gttcaatagg cagtgagctt gtaagacaga ttattagatt tttgccaagt
     3541 aaagtattat tattagatat agccgaatct cctttgtatg atattgagtt ggaaattaga
     3601 gaaaatttca aatttaataa tatagaaatc attattggcg atgttagaaa taaagacttg
     3661 ataaattata tttttgaaaa atataaacca gattttgttt atcatgctgc tgcgtataaa
     3721 catgttccaa tgatggaaaa tcatcctaac gaagctgtaa aaaccaatgt gaaaggaaca
     3781 aaagtagttg ctgaagcagc aataaaatat aaaactagaa aatttgtaat ggttagtacc
     3841 gataaggctg ttaaccctac aaatgttatg ggtgcaagca aacgaatagc agaaatagta
     3901 gttcaatctt tgtctaaaac ttcggaaaca caatttgtaa ctacacgttt tggaaatgtt
     3961 ttgggttcta atggttctgt tataccgcgt tttagaaaac aaatagaagc aggtggtcca
     4021 gtaacagtaa ccgaccccga aattactaga tactttatga caattccaga agcttgtcag
     4081 ttagttttag aagctggaaa catgggagaa ggcggagaag tatttatttt tgatatgggt
     4141 aaacctgtga aaattattaa tctagctaaa aaaatgattt cattatctgg cttaaaacca
     4201 gggaaagaca ttcaaattag tattactggt ttaagacctg gcgaaaaact ttacgaagaa
     4261 ttgttggcaa ataaagaaaa cacattacct acttatcatc ctcaaataat gattgctaag
     4321 ctaaaagaat atgatgctga tgaagtcttg cctgaaataa acatgcttat agaaaatgct
     4381 attgagaaaa atgataattt tgaactcgta aaaaaaatga aagaaattgt gcctgagttt
     4441 attagcaaaa attctgttta cgaagtttta gatacaaatt aaaaatcaat aaattttaaa
     4501 caaaaacttt tatagcttgt ggtatgatgc ttatctcata aggagcataa tcaataattt
     4561 caccatcagt ttcaacaaaa atatcttggt cagcttctat aaagaatttt ttggttttaa
     4621 acattcttac gcctttaact ttacctatgt ttccattgta taaccttctg atatttgcta
     4681 caattttaaa tttagacata tttttataaa tagtaacatt aagccaccca tcatcaatta
     4741 ttgcatcagg agtttgaatc attccaccac cagaaaattt gccatttcca atacttattg
     4801 ataaaaactt tccttcaaga atttcatcat ttatatgaat ttttaactcc caaggtttgt
     4861 aagtgatgag gctttttagc aaattaatca tatatgctaa gtttgttctt ttgcctcttt
     4921 ctttcatttg atttgttgct tttacaacaa ccgagtcaaa gcaaaaacct gcaatattta
     4981 taaaataagc ttttttgtct tcgttgtcag ccttatattt tattacacca acatcttgaa
     5041 atttagtgtt tttgctttta aatctatcta ttgctttttg ataatcgttg tcaaatttat
     5101 aatacttaga ccaatcattt cctgtaccca tttgaataat gcctaagcta atttcttgag
     5161 aattaacttc gttttgttta agaacagcgt ttgtaacttc gtttaaagtg ccgtcaccac
     5221 caagaattat aaaatttcta agtccaattt ttatttgttt ggaaacaatt tcttcggcat
     5281 gaccaatata ttcagtgaaa gcaaagtcaa aatctaaatt atttttattt ataacttcta
     5341 gcacattagt ccactcgcgt tcgccttcgg aattgccggc atttggattt atcaaaaaga
     5401 agtatttatt ttcgtaagtt ttattcaaaa tatttatttt tacagtttta tttttattat
     5461 ttttaaaatg caaaaataca aattattatg gcaaaaaaaa ttagactgat aaaatcttat
     5521 caaagcgaaa ttaacaaacc agaaacagaa tatcttgctg ttgtaaaaga atacaatgaa
     5581 aaaggcatgg aaattagact tgtagaatat acagaggata atgaaattga gtatgaacac
     5641 acccttgagt gcgatagtga ttttaatcca attagggaag aaataattag taaatctgat
     5701 gaatactcag agcagaaaac tttcaaatat gacgaaaata ataagttaat agaagaaaaa
     5761 attcaatatc aaaatgcttg ggaaagcata aaaaaatatg aaagagacca agaaaaacgt
     5821 tgtgttataa ttactacctt tgatgaagag ggcgaagttg aggaaagtag cgagatttat
     5881 tataatgaaa aaggcgaaat tattactcgt atcgaaaatg atgagtatgg aaaacaaaaa
     5941 aacaaggtta aaaacagttt tgacgaaaaa ggcatattaa tttcaaaaga agaatattca
     6001 aatactaaaa aacctgataa aatttatcat tatcattata atgaaaatgg cgatctttat
     6061 gctgttgaaa cccaaaactc acgaaatcgt ttgatagatt ggacaaaaat tgtttttgat
     6121 gataaaaatc gtcctattga acaagttaat atggcaggaa caaaacttgt tatcgaatat
     6181 aatgatgaag aaagtgaaat aacagaaaca tattatctag caaatggaac aatatataat
     6241 aaaacaaaat ataagaaaaa ttccgaagga cttacagaat atgaagaaac tagtgataaa
     6301 attactagat atgtttatga gtatttttag ggatattcgt taaattcaaa attttaattt
     6361 tgtcatttat ttattttgtt
//
LOCUS       DAOPNX010000117         6303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_13558_length_6303_cov_7.156050, whole genome shotgun sequence.
ACCESSION   DAOPNX010000117 DAOPNX010000000
VERSION     DAOPNX010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13558_length_6303_cov_7.156050"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="PKN53_10075"
     CDS             complement(<1..1261)
                     /locus_tag="PKN53_10075"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOD89335.1"
                     /translation="MNFIDLRVAKPLLNALEDLEYVYPTPIQQKCFRRITSGADVVGI
                     AKTGTGKTLAYLLPILSQLEYSNQKNPRVIILVPTRELVVQVCEEAEKISKYKSVRIK
                     GVFGGVNINTQKDYVFDDGADILVGTPGRLYDIAVTGVIRFSNVKKIVIDEVDEMLSL
                     GFRPQIEQIFEMLPNKRQHLMFSSSLSENVEEFIKSYFNSPEKIEVDLESTPVEKIKQ
                     TFFPVQNFNTKIEFLKVLLSQKDEFIKNLIFVADKRQADLVFDKLSSEFEDLGIIHSN
                     KSQNYRFNTIKNFEKRKIRSLIATDVVARGLDFSDISHVINFSVPENASDYIHRIGRT
                     ARAGKTGTSILMYAPYEEKYFEEIKKIAGENTKEESFPQGIPKIDSLLEFEKPAFTHK
                     QITKAPDISKGGGAFHEKRNINTKQKRK"
     gene            complement(1282..6279)
                     /locus_tag="PKN53_10080"
     CDS             complement(1282..6279)
                     /locus_tag="PKN53_10080"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOD89336.1"
                     /translation="MKSFYNILFSSLIFLAVYTNCVFSQNSQANWINYNQQYFKIQLA
                     QDGLHRIPYSSLLQAGIPVDNIDPRWIQIFFQGEEQYIYIHGEGTSGIFDPNGYIEFY
                     GQRNKSELDLEFYDNPNNCVNPDYSMYSDTSAYFLTWNFSTNNRRFTNVSDNNFEDYS
                     AYAQDYCYKNIRNNYTSRFYWGSTQNVFSKSEGWFDNAVINEETPRTKSINISNFYSG
                     NANTYFEIAVAGVPASAVTSLVPHHLKVEFLNETRINETYTGYNFIRKNIVLSSSLLA
                     NNISFKFTSNDATQPDIADRNAVSYINIKYPHTWDFENQNYFEFYLPKNNSSEKDYLE
                     ITNFNTSSSIILYDITNKQRISPVNNGGILKTLVNNTNSERFLVLTNSDGVKSVNKIS
                     KIGNNNKFIDYETQYQNANYIIITHSSLWAQAQQYANYRISTGYDVALIDIDQLYNQY
                     AWGVNKHPAAIRNFLKALFEISEMERHVFLLGKAIHTRLTRNAPEIYKECLVPSAGHP
                     ASDNIFTINFKDNNYYPIYPIGRLAAYDQEDVAEYLKKVMQYETAPTDEWMKTILHFG
                     GGANAAEQASFASYLNNYKNIIEDTLYGGKVSTFLKNSSEPIQITQSDSITNLINNGS
                     SIITFFGHAHAGGFDQNIDFPQNYNNTGKYPFIFANSCFAGDIHLRNQASVSEIWINE
                     KQKGAIAFLASIGDGIASYLNIYATELYKNIAYKNYYKTIGTQIKNTIKNILLNYPQN
                     TYVEVACHETALHGDPALVINSHEKPDLTISEDLIKFIPQEITTVADSFQVRTVIKNI
                     GRATADTFLLSINRTLPNGNSSEYNIPVYGCNYRDTVYFNLPVDRLNGPGLNKLHLFV
                     DAGNEIDELNENNNQANIDFLIKSADLFPIYPYKYSIIPSKNASLIASTGDPFLNSRN
                     YIFQIDSTDLFNSPILQTKTTNSTGGIVKWDLPFDLVENRVYFWRIAYEHNNPDSIFW
                     KESSFVYIQGEEGWSQAHFFQYKENDFMFLNYDRPARKLNYVNYPKKLHCHNQGNYGE
                     NNYTIVRWTLDNSYNNGLGAFGNCGTAAAMLVVVIDPETILAWASDYQNFGHRNYPKC
                     FSSSQENYYFSFSTDPVSLDSMNNMISNYVPDGHYLLIYSWGNGNFSNWPPQLKQTFY
                     DLGSLQIDNVPNGHPYIFFTKKGTISSTMETYGTSPTDKIDLYVDLYRDFDNGSMKSV
                     KVGPAAEWKSFNWEYFALENPHQDSINIKVFGIDNFGNETQVMSPITPENLEIFQLQD
                     SINYQTFPNLKLEFYSKDQANKTSAQLKKWQLKFKGVPETAIDPKSGFFFCCDTIQEG
                     DEVYFSIATKNISDLDMDSLDVIYWVKNSDNETTLTKRKRLRPHPAGDILKDTISFSS
                     MGMKGIYSIWVEFNPTNPQTGIYYQAEQYHFNNIANKYFFVQGDITNPLLDVSFDGRY
                     IMNGEIISAKPEILIKLKDENKYLALNDTSLFRIYLSDLTTGTEKRVYFGLQENPEET
                     IEWTPAALPDNSCKILYKPIFTKDGMYRLKVQAVDVSGNESGENDYSIDFEIINKATI
                     SRLLNYPNPFSTSTRFVFELTGSEIPDELRIDIFTVTGTLVKTIFLDEFGSIRIGKNI
                     SEYAWDGKDMYGDQLANGVYFYQVSIKINGKEIELRETEADKYFKKEIGKMYLLR"
BASE COUNT         1938 a          966 c          983 g         2416 t
ORIGIN      
        1 tcttcctttt ttgcttagta ttaatatttc ttttttcgtg gaaagcaccg cctcctttag
       61 aaatgtctgg agcttttgta atttgtttat gtgtaaaagc tggtttttcg aactccaata
      121 ggctatctat ttttggaatt ccttgaggaa aactttcttc tttagtgttt tcgcctgcaa
      181 tttttttaat ttcttcaaaa tatttttctt cgtaaggagc atacattaaa attgaagttc
      241 ccgttttgcc tgctcttgca gttcttccaa ttctatgaat atagtcagaa gcattttcag
      301 gaacagaaaa attaataaca tgacttatat ctgaaaaatc taatccgcga gcaacaacat
      361 ctgtagctat aagagaacgt atttttcttt tttcaaaatt tttaatagta ttaaaacgat
      421 aattttgtga tttgttagaa tgtattattc ctaaatcttc aaattcgcta cttagcttgt
      481 caaatactaa atctgcttgt cgtttatctg ctacaaaaat caaatttttt ataaactcat
      541 ctttttgaga aagtagcact tttaaaaatt caatttttgt attaaaattt tgaacaggga
      601 aaaatgtttg tttaattttt tctacaggcg ttgattctaa atcaacttct attttttctg
      661 gtgagttaaa gtatgatttt ataaattctt ctacattttc gcttaaactt gatgaaaaca
      721 ttaaatgttg gcgtttgttt ggaagcattt cgaaaatttg ctctatttgt ggtctaaagc
      781 ctaatgataa catttcgtca acttcgtcaa ttacaatttt ttttacattg ctaaatctaa
      841 taactccagt aacggcaatg tcgtaaagtc tgccaggtgt tccaactaaa atatctgcac
      901 catcgtcgaa aacataatct ttttgtgtat ttatatttac tccaccaaac actcctttta
      961 ttcttacgct tttatattta gagatttttt cggcttcttc gcaaacttgc acaaccaatt
     1021 ctcgagtggg aacaagaata ataactcttg gatttttttg attagaatat tctaattgac
     1081 ttaaaatagg caaaagataa gctaaagttt ttccagttcc tgtttttgct attcctacaa
     1141 catcagctcc agaggtaatt cgtctaaagc atttttgctg aataggagtg gggtaaacat
     1201 attctaaatc ttctaatgcg tttaataatg gtttggctac tcgtaggtct ataaaattca
     1261 ttgttcttta ttcttaatta tttatctcaa caaatacatt tttccaattt ctttcttaaa
     1321 atatttgtct gcttctgttt ctcttaactc aatttctttt ccatttattt taatgcttac
     1381 ttgataaaag taaactccat ttgcaagctg gtcgccatac atatcttttc cgtcccaagc
     1441 gtattcgcta atgtttttcc cgatacgaat gcttccaaat tcgtctaaaa atattgtttt
     1501 tacaagtgtg cctgtaactg taaaaatatc aattcttaat tcgtctggaa tttcgctacc
     1561 tgtaagttcg aacacaaaac gtgttgaagt agaaaatgga tttggataat ttagcaagcg
     1621 gcttattgtt gctttgttta ttatttcaaa atctatacta taatcatttt ctccagactc
     1681 gttacccgat acgtcaacag cttgaacttt aagcctatac attccatctt tagtgaaaat
     1741 tggtttgtat aatattttgc aagaattatc tggcaaagct gctggtgtcc attcaatagt
     1801 ttcttcagga ttttcttgta agccaaaata aactcgtttt tcagttcctg ttgttaaatc
     1861 gcttaggtaa attctaaata acgaagtgtc gtttagtgcc aaatatttat tttcatcttt
     1921 gagttttatc aatatttcag gttttgccga aataatttct ccgttcataa tatatctacc
     1981 atcgaaactt acgtctaaaa gaggatttgt aatatcgcct tgaacaaaga aatatttatt
     2041 tgcaatatta ttaaaatgat attgttctgc ttggtaataa attcctgttt gcggatttgt
     2101 aggattaaat tctacccaaa ttgagtatat tcctttcatt cccatgcttg aaaagctaat
     2161 agtatctttt aaaatatctc cagcagggtg gggtctaagt ctttttcttt ttgttagggt
     2221 tgtttcgtta tcagagtttt ttacccaata aattacatca agcgagtcca tatctaaatc
     2281 gctaatgttt tttgttgcaa tagaaaaata aacttcatct ccttcttgaa ttgtgtcgca
     2341 acaaaagaaa aatcctgatt ttgggtctat tgcagtttct ggaactcctt taaattttaa
     2401 ttgccatttt ttaagttgag ctgaagtttt atttgcttgg tctttactgt aaaattctaa
     2461 ttttaagttt ggaaaagttt gataatttat agaatcttga agttggaaaa tttctaaatt
     2521 ttcaggagta attggcgaca ttacttgagt ttcgttacca aaattatcaa ttccaaacac
     2581 ttttatattt atagaatctt gatgagggtt ttcgagagca aaatattccc aattaaaaga
     2641 tttccactct gctgctggac caacttttac gcttttcatg cttccgttat caaaatctct
     2701 gtataagtcc acatataaat caattttatc agtaggcgaa gttccataag tttccatggt
     2761 tgaggagata gtgccttttt ttgtaaaaaa tatgtatgga tgtccatttg gaacattgtc
     2821 gatttgtaaa gagcctaaat cataaaatgt ttgttttagt tgaggcggcc agttagagaa
     2881 atttccattt ccccatgaat atataagtaa ataatgtcca tctggaacat aattgcttat
     2941 catattattc atagaatcta agctaactgg gtcggtacta aaggaaaaat aataattttc
     3001 ttgagaactt gaaaaacatt taggataatt tctatgtcca aaattttgat agtctgaagc
     3061 ccatgctaaa attgtctcag ggtcgattac cacaactagc attgctgcag ctgtaccaca
     3121 atttccaaaa gctcctaatc cattattata agaattgtcg agtgtccatc taacaattgt
     3181 gtaattattt tcgccataat ttccttgatt gtggcaatgt agtttttttg gataatttac
     3241 ataattaagt tttcgtgcag gacggtcata atttaaaaac ataaagtcat tttctttgta
     3301 ttggaaaaaa tgagcttgcg accatccttc ttcgccttgt atataaacga aagaagattc
     3361 tttccagaaa atactatcag gattattatg ttcataagca attctccaaa agtaaactct
     3421 gttttcaaca agatcgaaag gcaaatccca ttttacaatt cctccagttg agttagtcgt
     3481 ttttgtttgt agaatagggc tgttaaacaa atctgtgcta tctatttgga aaatataatt
     3541 tcttgaattt aaaaatggat cgccagttga agcaattaaa ctagcatttt ttgaaggaat
     3601 aatagaatac ttgtaaggat aaatcggaaa taaatcagca gattttatta gaaaatcaat
     3661 atttgcttga ttattatttt catttaattc atcaatttca tttccagcat caacaaaaag
     3721 atgaagtttg tttagtccag gtccatttaa tctatcaaca ggtaaattga aatatacagt
     3781 atctctgtaa ttgcagccat atacaggtat gttatactca ctactgttgc cgtttggcaa
     3841 agttctattt atagaaagta aaaatgtgtc ggcagtagct ctacctatgt ttttaattac
     3901 agttcttact tgaaaactat cagctacagt agtaatttct tggggaataa attttattaa
     3961 atcttcggaa attgttaaat ctggtttttc gtgagaatta attactaagg cagggtctcc
     4021 gtgtagggca gtttcgtggc aagcaacttc aacgtatgtg ttttgaggat agtttagtaa
     4081 aatatttttg attgtgtttt ttatttgagt tccaatagtt ttatagtaat ttttgtaagc
     4141 aatatttttg tataattctg tagcataaat attcaaatac gaagcaattc cgtcacctat
     4201 tgaggcaaga aatgctattg ctcctttttg tttttcgttt atccaaattt cgctaacgct
     4261 agcttgattt cttaggtgta tgtcgccagc aaagcaagag tttgcaaaaa taaacgggta
     4321 tttcccagta ttgttgtagt tttgtggaaa gtcaatgttt tggtcaaaac caccagcgtg
     4381 ggcgtgtcca aaaaaagtta taatgctaga accattattt attaaatttg taattgagtc
     4441 agattgagtt atttgtattg gttcagaaga attttttaaa aaagtactta cttttccacc
     4501 atataatgtg tcttcaataa tatttttata attattcaaa tatgatgcaa aacttgcttg
     4561 ttcagctgca tttgcacctc ctccaaaatg taatatggtt ttcatccatt cgtctgttgg
     4621 agcagtttcg tattgcatta cttttttcaa atattcagca acatcttctt ggtcgtatgc
     4681 tgcaagtctt cctataggat atattggata atagttgttg tctttaaaat ttatagtgaa
     4741 aatattatca ctagcaggat gtcctgctga aggaaccaag cattctttat aaatttcggg
     4801 agcatttcgc gttaatcgtg tgtgaattgc tttcccaagt aaaaatacat gtctttccat
     4861 ttcggaaatc tcaaacaaag ctttaagaaa gtttcttatt gcagcgggat gtttgttcac
     4921 tccccaagca tattgattgt ataattggtc aatgtcaatt aaagctacat catatcctgt
     4981 tgaaattctg tagtttgcgt attgttgtgc ttgtgcccat aatgacgagt gagtgattat
     5041 tatataattt gcattttgat attgtgtttc gtaatcaata aatttattgt tatttccaat
     5101 ttttgaaatt ttatttacag attttacacc atctgaattt gttaaaacta aaaatctttc
     5161 tgaattagta ttgtttacaa gtgtctttaa aataccacca ttgtttacag gactaattct
     5221 ttgtttgttt gtaatatcat ataatattat agatgatgaa gtgttaaaat ttgtaatttc
     5281 caaataatct ttttcagaag agttgttttt tggtaaataa aattcaaaat aattttggtt
     5341 ttcaaaatcc caagtatgtg gatattttat gttaatgtat gacacagcgt ttctgtcggc
     5401 tatgtcaggt tgtgtagcat catttgatgt aaatttaaaa ctgatattat ttgcaagtag
     5461 cgaagaactt agtacaatgt tttttctaat aaagttatat cctgtatatg tttcgtttat
     5521 tcttgtttcg tttaaaaatt ccacttttaa atgatgagga acaagagaag taaccgcact
     5581 tgccggcact cctgctactg caatttcaaa ataggtgttt gcatttcctg aataaaaatt
     5641 tgaaatgttt atgctttttg ttctaggagt ttcttcattg ataacagcat tgtcgaacca
     5701 gccttcgctt tttgaaaaaa cattttgagt agatccccag taaaatctcg aagtgtaatt
     5761 atttctaatg tttttatagc aataatcttg agcataagcc gagtaatctt caaaattatt
     5821 gtcagaaaca tttgtaaatc tacgattgtt tgtggaaaaa ttccaagtta aaaaatatgc
     5881 tgaagtgtcg ctatacatgc tgtaatctgg atttacacaa ttatttgggt tatcgtaaaa
     5941 ttccaagtct aattctgatt tatttctttg cccataaaat tctatatatc cgttagggtc
     6001 gaaaatgcca gatgttcctt ctccatgaat gtaaatatat tgttcctcgc cttgaaaaaa
     6061 tatttgtatc catcttgggt caatattatc aacaggtatt cctgcttgta ataaacttga
     6121 ataggggata cgatgtagtc catcttgagc aagttgaatt ttaaaatatt gttggttgta
     6181 gtttatccag tttgcttgag aattttggga aaatacgcag ttagtgtaaa ctgctagaaa
     6241 aattagactt gaaaataaaa tattataaaa agatttcatc ttatataatt cccaaaaata
     6301 aaa
//
LOCUS       DAOPNX010000118         6195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_13854_length_6195_cov_7.406189, whole genome shotgun sequence.
ACCESSION   DAOPNX010000118 DAOPNX010000000
VERSION     DAOPNX010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13854_length_6195_cov_7.406189"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            445..1116
                     /locus_tag="PKN53_10085"
     CDS             445..1116
                     /locus_tag="PKN53_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89337.1"
                     /translation="MKKTYFFILLLCVTKLSLSQSDSLVKCDFSFEFKPGLYLQFSDF
                     RSNSPIPFESVVYPDYSHKDFYYNLDTAKTIIFNDKHGLLIKKQISEVWGFSKNGKPY
                     IYWSNKANLIPFVGSISHFITTINTIRHISPEPYYGSYYYNPYYYSPPMSRTYQTEEL
                     KQFIIDMETGNILEYSVKNVESILKREPQIYDEFSKLRKRKKTKQLFYYVRLYNEKRV
                     LMMNE"
     gene            1137..1994
                     /locus_tag="PKN53_10090"
     CDS             1137..1994
                     /locus_tag="PKN53_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89338.1"
                     /translation="MLRLVIFIFLINSYLIGFAQINISRGSQAEINTFLNSKTYIVLN
                     NTLMSDYNMEMKEAILKHWTITDYEFITESEFKKHKSNPKNSFLMINQVYFEKDKTQT
                     LFDFLILCLGGNYKTVNDMPTLCAIPLCYHGALQEAYAYKLGLMVKFTQLHIKTCKNN
                     PTISEDKLAEYYMNKTGSPKTKTFYLLKEEVEPEIRTKNSFATAYPFNFEYADKEKIK
                     NLIDNNDEDAIILHILGPQTSNTLSYCIKIIIDTQEGNIYYYDMHKLSNKNADRLLKS
                     DLKKLASKK"
     gene            1991..3289
                     /locus_tag="PKN53_10095"
     CDS             1991..3289
                     /locus_tag="PKN53_10095"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOD89339.1"
                     /translation="MNYQEVIEFMFNSLPMYQRVGAAAYKANLDNTLAFDKITGSPHK
                     KYKTIHIAGTNGKGSVSHSLASILQEAGYKTGLYTSPHLTDYRERIRVNGKKIEESFI
                     TEFINSHKNFIKNVKPSFFEMSVALAFKYFELQQVEVAVIEVGMGGRLDSTNIILPEL
                     SVITNIGLDHTQFLGNSLAEIAGEKAGIIKQNIPVVIGERQKETEEVFLKKAKKKNSK
                     ISFASDNYACKILNSNLKYSSFEVEKKTSDKSYTLNFNLSGKYQEKNLATILETVDNL
                     RNQGFCISDENLKQGLENLIKNTGFHGRWETLNAEPLTICDTGHNKDGLSYTIEQLLS
                     LEFKKLRFVLGFVNDKNVDEVLKLFPKNAEYYFCKAQIPRALDEKTLLEKANLVSING
                     KSFENVSSAYHAAKKDADKNDVIFIGGSTFVVADLLQEFA"
     gene            3344..>6195
                     /locus_tag="PKN53_10100"
     CDS             3344..>6195
                     /locus_tag="PKN53_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOD89340.1"
                     /translation="MKVKYLLKGKKLWILISILLAVVITICVVLLTKEKPKSPETGKA
                     FFYNFPAKICDSITDLRTTDEQIASLFILANSQDSLIATKDFFSGFFFFEYNGQTLLN
                     QQNDSCTNKLIRGIHEKELRNLFADSIKTFSSELLNTISDTVVLLNYYNYQTDLLVKH
                     NINALLIDANIPEKLDSIAVKLISDRLKIKLDILHKNNILSILHLDKIDTKDEKSEQY
                     LQELYSNIINSGLCGIIISESEQLNYFSDFDGLVFKLTKLGETINENLICSTNAFILY
                     DEKDINSLKNDIKKHTKKKDIFLKSSKFLRASMWANQKTAQDSTKQLLDLNLLEARIT
                     EASITLLKNSNNLIPISDINQKIHILIASKSKYTDFINIISNYINNYTVSYFNTDSKK
                     PPVIPRATNTLIVLYDTNFSDSIPNLLKTSLLEFKQQKIFLNFGKLSSEICEIDADAI
                     LQTYDDSFFSQSFVAQAIFGGVAIKGSLANKLNDSINYGHGIKTTKTRLKYSVPEEVG
                     LNSEILLKIDSILNYAISSGVFPGCQIFVAKNGVVVHNKSYGYLDYSKTNSVKSSVLY
                     DVASLTKICATTLATMKMYETDKINLNDKLSKYFKNTSIDYGNIKPDTLIFIDTISIK
                     GKSLSEIEDLVQDKDTIHLNDTLVELTEIILTKTTPKSNIFQVPVRALLVHQSGISPS
                     LPILKYMFYLKSYKEFLQNEHLKTDSLDFLDLDDITQEEAFDFYYSKKWIKDSAELKI
                     AENMYLKKRWQDSLYEDVKRLGTFKREVYQYTDMNMILMQAIIDTTNEKSLDVYVKTE
                     FYKNLGMNNTTFLPLEQKIPKTRIAPTENEKFWRKQVIHGNVHDPSAAMLGGISGNAG
                     LFSTASDLGILGQMWLNGGTYGGKRYLKQNTIKMFSSTQPENHRGLGFDKVSIKNLNA
                     ASAPATTFGHTGFTGCAMWIDPENEIVYVFLSNRIHP"
BASE COUNT         2432 a          837 c          903 g         2023 t
ORIGIN      
        1 tggagattat ttgtaaaaat gagcaagaca acttagttga tttaaaaagt gaaaacgaca
       61 ttgttgtaac acttaaaaaa atagaaaaat aactatttat actgttaaaa gggaaatggt
      121 aaaagtcgaa ccgagcttgc gagctcaccg aaggtaaagt taaaagtggt ctgcgggttt
      181 ggcttttctt tatgaacttt ttactttata aactttcaac tgaatttatt gtattgcaaa
      241 taatgcgaaa aacaggattt tgctacataa aaatttcaaa atacgctttt aaatgcacaa
      301 ataaaaagag tgtttgttga tttgttttta aaacatttaa gcacataaac ataaatttta
      361 taatatttga ttttctaaaa tattaaaaat tacttcacaa aattctataa aaaattagaa
      421 aactacatta tctttgacta aaatatgaaa aaaacttact tctttatatt gcttttatgt
      481 gtaactaaac taagtctttc gcaatcagac agtttggtta agtgcgattt tagttttgaa
      541 tttaaacctg gtttgtattt gcagttttca gattttagaa gcaattcgcc aattcctttt
      601 gaatcagttg tttacccaga ttattctcat aaagattttt attataattt agatactgca
      661 aaaaccataa tctttaacga taaacacggt ttgcttatca aaaaacaaat atctgaagtt
      721 tggggatttt ctaaaaatgg aaaaccatat atttattggt ctaacaaagc aaatttaatt
      781 ccttttgtag gttctatttc tcattttatt acaacaatta atacaattcg ccatataagt
      841 ccagaacctt attatggttc ttattactac aatccttact attattcgcc accaatgtcg
      901 agaacctatc aaaccgaaga attaaaacaa tttataattg atatggaaac tggaaatatt
      961 cttgaatata gtgtgaaaaa tgtagaatca atattaaaac gagagccaca aatttatgat
     1021 gaattttcaa aattaaggaa aagaaaaaag actaaacaac tgttttatta cgttagactt
     1081 tataacgaaa aacgagtttt aatgatgaat gagtaaattt ttaaaaacca tgtataatgt
     1141 taagattagt aatttttatt tttttaatta actcctattt aataggtttt gcacaaatta
     1201 atatttctcg cggaagtcaa gcagaaatca atactttttt aaactcaaaa acttatattg
     1261 tactaaacaa cacattaatg tctgactaca atatggaaat gaaagaagcc atcttaaaac
     1321 attggacaat aacagattac gagtttatta ctgaatcaga gtttaaaaaa cacaaatcaa
     1381 atcctaaaaa ttcttttttg atgataaacc aagtttattt tgaaaaagac aaaacacaaa
     1441 cattgtttga ttttttaatt ttatgtttag gtggaaatta caaaaccgta aacgacatgc
     1501 caactctttg tgccataccg ctttgctatc atggtgcttt gcaagaagca tatgcttata
     1561 aattaggatt aatggtaaaa ttcactcaat tacatataaa aacttgcaaa aataatccaa
     1621 ctataagcga ggataaatta gcagaatatt atatgaataa aacaggtagt cctaaaacca
     1681 aaacttttta tttattaaaa gaagaagttg aacctgaaat tagaacaaaa aattcttttg
     1741 ctacagcata tccatttaat tttgaatatg cagacaaaga aaaaattaaa aacttaattg
     1801 acaacaatga tgaagatgca ataatcctac atattttagg accacaaaca agcaacacac
     1861 tttcttactg tataaaaata attattgaca ctcaggaagg caatatttat tattacgaca
     1921 tgcacaaact gagcaataaa aatgcagaca gattattaaa aagcgattta aagaaattag
     1981 caagtaaaaa atgaattacc aagaagtaat tgaatttatg ttcaactctt tacctatgta
     2041 tcaaagagtt ggggcagccg cttataaagc aaatttagac aatactctag catttgataa
     2101 aattactggc tcgccacata aaaaatacaa aaccattcat attgcaggta ctaatggcaa
     2161 aggttctgtt tcgcatagtt tagcatcaat tttacaagaa gcgggctaca aaactggtct
     2221 ttacacctcg cctcatctta ccgattatcg cgaaagaatt agagtaaatg gaaaaaaaat
     2281 agaggaaagt tttattacag agtttataaa tagccataaa aattttatca aaaatgtaaa
     2341 accttctttc tttgaaatgt cggtagcctt agcttttaaa tattttgagt tacaacaagt
     2401 tgaggtcgct gttattgaag ttggaatggg cggaagattg gattccacaa atattatttt
     2461 gccagaatta tcagtaatta caaatattgg attagaccac acacaatttt taggtaatag
     2521 tttggctgaa attgccggcg aaaaagctgg aataataaag caaaatattc ctgtagtaat
     2581 tggagaaaga cagaaagaaa cagaagaagt tttcttaaaa aaagccaaaa aaaagaacag
     2641 taaaataagt tttgcatcag ataattatgc ttgtaaaatt ttaaactcaa atcttaaata
     2701 ctcaagtttt gaggtggaga aaaaaacaag tgataaatct tacactttaa attttaactt
     2761 atcgggaaaa tatcaagaaa aaaacttagc tacaatactt gaaactgttg ataatttgag
     2821 aaatcaagga ttttgtattt ctgatgaaaa tttgaaacaa ggtttagaaa atcttataaa
     2881 aaacactggt tttcatggca gatgggaaac tttaaatgcc gaaccactaa caatctgtga
     2941 cacaggacat aacaaagatg gattaagcta tactatcgaa caattattaa gtcttgagtt
     3001 taaaaaacta agatttgtgc ttggttttgt taatgataaa aatgttgatg aagtattaaa
     3061 gctatttcca aaaaatgctg agtattattt ttgtaaagca caaattccac gagctttaga
     3121 tgaaaaaaca ctgttagaaa aagcaaattt ggttagtata aacggcaaat cttttgaaaa
     3181 tgtaagttcg gcttatcatg ctgctaaaaa agatgctgat aaaaacgatg tgatttttat
     3241 tggaggaagc acttttgttg tggctgattt gttgcaagaa tttgcataaa gttttacgaa
     3301 ttgatatata tttatatatt tttgcaaaaa taaaaaaaac gaagtgaaag ttaagtattt
     3361 gctgaaaggg aaaaaactat ggattttaat atcaatactt ttagcagtag tgataacgat
     3421 ttgtgtagtt ttattaacaa aagaaaaacc taaaagtcca gaaactggta aagcattttt
     3481 ttataatttt cctgcaaaaa tttgtgattc aattactgat ttgcgcacta ctgatgagca
     3541 aattgctagt ttgtttattt tagcaaattc acaagatagt ttaatagcaa caaaagactt
     3601 tttttctggc ttttttttct ttgaatataa tggtcaaact cttcttaatc aacaaaatga
     3661 cagctgtact aataaattaa ttagaggaat tcacgaaaaa gaacttagaa atttatttgc
     3721 agattccata aaaacttttt catctgaact tttaaataca attagcgata ctgttgttct
     3781 gcttaattat tataattacc aaacagactt gcttgttaaa cacaatataa acgcattgct
     3841 tattgatgca aatattcctg aaaaattgga ttctatagct gtgaaattaa tttcagatag
     3901 gctgaaaatt aaattagaca ttttacataa aaacaatatt ctgagcatct tgcatttaga
     3961 taaaattgac acaaaagatg aaaaatcaga acaatattta caagaacttt attcaaacat
     4021 aattaattcg ggtttatgtg gtataataat ttcagaaagt gagcagctta attatttttc
     4081 agattttgat ggacttgttt ttaagttaac taaactgggt gagacaataa atgaaaatct
     4141 tatttgctca acaaatgcat ttattcttta cgatgaaaaa gatattaata gtttgaaaaa
     4201 cgacattaaa aaacatacaa aaaagaagga tatatttcta aaaagttcta agtttttacg
     4261 tgctagcatg tgggcaaatc aaaaaacagc acaagacagc acaaaacaat tgcttgacct
     4321 aaatttatta gaagcaagaa ttacagaagc atctattaca ttgctaaaaa actctaataa
     4381 tttaattcct atttcagata taaatcaaaa aattcatatt ttaattgcca gtaaatcaaa
     4441 atacactgat tttattaata ttatttcaaa ttacattaat aattataccg tatcgtattt
     4501 taatactgat tctaaaaaac cgcctgttat tccaagagct acaaacactt taatagtttt
     4561 gtacgacact aacttttcgg attctattcc taatctttta aaaacaagtc tattagagtt
     4621 taagcaacaa aaaatatttt taaattttgg caagcttagt tctgaaattt gcgaaataga
     4681 tgcagatgca atcttacaaa cttatgacga cagctttttc tcacaatcat ttgttgcaca
     4741 agctattttt ggaggagttg ctattaaagg aagtcttgca aacaaattaa acgacagcat
     4801 aaactatgga catggaataa aaactacaaa aactagactt aaatattctg tacctgaaga
     4861 agtgggttta aattccgaaa tattattaaa aattgatagc attttaaatt atgctatatc
     4921 tagtggtgtg tttcctggat gtcaaatttt tgttgctaaa aacggagttg ttgttcataa
     4981 taaatcttat ggttatcttg attattcaaa aacaaattct gtaaaaagtt cggttttata
     5041 cgatgtggct tctttaacaa aaatttgtgc tacaactctt gctacaatga aaatgtatga
     5101 aactgacaaa attaatctaa atgacaaatt atctaaatat tttaaaaaca cttctattga
     5161 ttatggaaat ataaaacccg acactttaat ttttatcgac actatttcta ttaaaggaaa
     5221 atccttatct gaaatcgaag acttggtgca agataaagac actattcatt taaacgacac
     5281 attagtagag ctaacagaaa ttatccttac aaaaacaaca cctaaatcta atatttttca
     5341 agttcctgtt cgagctttac ttgttcatca aagtggaata agtccttcat tgcctatttt
     5401 aaaatacatg ttttatttaa agtcttataa agaattttta caaaacgaac atttaaaaac
     5461 cgatagttta gattttcttg atttagacga tattacacaa gaagaagctt ttgattttta
     5521 ttatagtaaa aaatggatta aagattctgc tgaactaaaa attgcagaaa acatgtattt
     5581 aaaaaagcgt tggcaagata gtttatatga agatgtaaaa agattgggaa cctttaagcg
     5641 agaagtttat caatacactg atatgaatat gattttaatg caagcaatta ttgatacaac
     5701 aaatgaaaaa agtttagatg tttatgtaaa aactgaattt tacaagaatt taggaatgaa
     5761 taatacaaca tttttacctt tagagcaaaa aattccaaaa acaagaattg caccaaccga
     5821 aaacgaaaaa ttttggcgaa aacaagtaat acatggcaat gtacatgatc cttcggcggc
     5881 aatgctaggt ggaatatcag gaaatgctgg cttattttct accgcctctg acttgggaat
     5941 acttggacaa atgtggctaa atggaggaac ttatggagga aaaagatatt taaaacaaaa
     6001 tactataaaa atgttttctt ctactcaacc cgaaaatcat agaggattgg gctttgataa
     6061 agtgtcgata aaaaatctaa atgcagcttc agcaccagca acaacatttg gtcatacagg
     6121 ttttactggc tgtgctatgt ggattgaccc agaaaatgaa attgtatatg tgtttttatc
     6181 aaaccgcata catcc
//
LOCUS       DAOPNX010000119         6170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_13944_length_6170_cov_6.807195, whole genome shotgun sequence.
ACCESSION   DAOPNX010000119 DAOPNX010000000
VERSION     DAOPNX010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13944_length_6170_cov_6.807195"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(391..2052)
                     /locus_tag="PKN53_10105"
     CDS             complement(391..2052)
                     /locus_tag="PKN53_10105"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOD89341.1"
                     /translation="MSTLNFKEIHNKVDANLKECKTLIVNADRKTLIGDAVKYHECIV
                     SKVGNLATWTAPDSTGRSPKDTLFVKRPQSQIHIDWNSPNNLPATESLFEEIFQDAME
                     KMRKYERIYINDRVIGADSEYALPVKLITPQALTSVFGDNMFRPVPKDLNNSCFAEKP
                     FYLISLPFDKLDEKKYEWKLRKLPNGKTTDKIVLIDFDNRIGIIIGSAYLGSVKKTMF
                     TVMNYYLPFQDVLPLHCSANEGKDGKTALILGLSGTGKTTLSADPRRALLGDDEHGWS
                     NKRVANFENGCYAKMININPAKEPDIFEAVMHKDDYLNHGVIVENAMIYPNGEFDFDD
                     ERLTPNSRASYPLTFLNNIKESSVGGHPSTILFLTADATGVLPPISRLDRNQAMLWFL
                     MGYTSKLAGTETGVVEPQTTFSRFFGAPFMPLLPKVYTKMLGDKMEEYDTSVYLINTG
                     WSGGKYGVGQRIDITATRAMVEAALNGDLRNVEYEYDSLFHLNIPKTCPDVDSSILFP
                     ENTWTNKEEYRKTAKALAKEFSDHFDKTYGKNDIAPEIIRNCPGK"
     gene            2231..2425
                     /locus_tag="PKN53_10110"
     CDS             2231..2425
                     /locus_tag="PKN53_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89342.1"
                     /translation="MNIQINNKSFQIFSGAKVIDAVRMYFTEIGKDFEIDKIVVEDKF
                     GNRLEFDGRLRNNKSLFIKF"
     gene            2430..3893
                     /locus_tag="PKN53_10115"
     CDS             2430..3893
                     /locus_tag="PKN53_10115"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOD89343.1"
                     /translation="MNIKKTYRLFFSIAIILAIFQTYSCKPKVEEENKKVVILAVNDM
                     HANIDNFPRFAFIVDSLREIYPDLLLVSAGDNQSGNPANDQYPQKGMPITELMNMIKF
                     DVCAVGNHEFDTKQDGFKYIIDNSKFDYISANIKPSSNLGLNIKPFKTIKMPNGLKIA
                     FTSVISLNENGIPDSHPNNLTDIKFTDPFTTAKDYLYLKDEHDLLIFVNHFGFEEDVE
                     LAEQFPKGKVDLIIGGHSHTVVEKEQIFNDILITQAGNKLKYCTLIELEVEKDYSVKK
                     SMKLIPINKNGEIIAEFQEKVDEYNDSPFLNEKIAEAEDDFTSYEQVGYLMGDALRLN
                     LNTDIAVVNPGGVRIDKLDKGDINVRDVYSMDPFGNEAVIFNLTGNEIKAMYLSAFTI
                     DEYLPIYPSGIKVKYILKDKKTIKDVIFYTESGEDLDMEKTYSVAMNNYMASVYNYEH
                     ADSGTGQFVTTAENMIDYLKNLETVPSYKNEKRVELK"
     gene            3984..6095
                     /locus_tag="PKN53_10120"
     CDS             3984..6095
                     /locus_tag="PKN53_10120"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOD89344.1"
                     /translation="MRKTTILLLSSIILFTMSCKQKTNEVSDNPLLQEWDTPYGVPPF
                     DKIKIEHYKPALEAAMQEQKDNIQKIIDNKEAPNFENTIAALEYSGLTLSKVAGVFFN
                     LLSSDTNEELQKIAQEISPKLSNHSDEIIMNPELFAKIEEVYKNKDNSNLNKEQLRVL
                     ELTYRDFVRSGAKLNDADKKRLMEINERLSLLALQFGDNLLAETNGYKLIIDKEEDLA
                     GLPEGLREAAAITAKENGEEGKWMFTLHNASVMPFLQYADNRELRKDIQQAYINRCNN
                     NNEYDNKKIILELVELRIERAKLLGYENHSQYILDDNMAKTPEAVYELLNQLWTPALE
                     LAKEEAELYREMMKRDGIQDDLQAWDWRYYTEKVRKEKYNLDEEELKPYFKLENVRDG
                     VFDVSNKLFGLSFVKSNEIPIYHPDVEAYEVKDENNEVVAVLYMDYFPRPSKSGGAWM
                     NDYRGQYVYEGKNVIPIITINCNFTKPTSTAPSLLTFDEAQTLFHEFGHGLHGMLSKC
                     TYPSISGTNVPRDFVELPSQLMEHWASEPEVMKSYARHYQTNEPIPDELIKKLEDAEH
                     FNQGFATTEFLAAALLDLYYHNLTEMNFSDVLVAEKEALEAIGLIPEIVVRYRSPYFS
                     HIFAGGYSSGYYSYIWSEVLDSDAFALFKEKGIFDPATAKSLKDNIYSNGHKDDPMTM
                     YVNFRGREPKIDALLEGRGLK"
BASE COUNT         2213 a          950 c          999 g         2008 t
ORIGIN      
        1 gcaatccgag tgcttggtaa tgttacactc ggattacaaa tccgagcgag cggggttgtg
       61 gagaaatccg agcgagcggg gttgtgggga aatccgagcg agcaagggaa tatttacatt
      121 tgttgaaatt agaatagata agtcagttaa aagttaaaag cggttttatg tttggctttt
      181 ctttacaaac tttttacttt ataaactttt aactgatttg gggtgcaata aattaaaatt
      241 tgtataaata agaaacggcg acatgcgccg tctctactat actgcataat ttattgaatt
      301 accaactttt aactaaaatc ttccagacac gtcagcccct tttaacactt caactacgtt
      361 cagtgcatcg cttttaactt ttaactgact ttattttcca ggacaattcc ttattatttc
      421 tggagcgatg tcatttttgc cataagtttt atcaaagtgg tcagagaatt cttttgcaag
      481 tgcttttgca gtttttctat attcttcttt atttgtccaa gtattttctg ggaataaaat
      541 gcttgaatcc acatctggac aagtttttgg aatattcaaa tggaaaaggc tatcatactc
      601 atattctaca tttctaagat cgccgttaag agcagcttca accatagctc ttgttgctgt
      661 aatatcaata cgttgtccaa caccatactt accacccgac catcctgtat ttatcaaata
      721 aacagaagtg tcgtattctt ccattttatc gcctaacatt tttgtgtaaa cttttggtaa
      781 taaaggcata aaaggagcgc caaagaatct tgagaaagta gtttgaggct ctacaactcc
      841 tgtttctgta cctgccaact tagatgtgta acccattaag aaccagagca ttgcttgatt
      901 tctatcaagt cgtgaaattg gaggtaaaac acctgtagca tcggctgtta agaataaaat
      961 tgtagatggg tgtcctccaa cacttgattc tttaatatta tttaaaaatg ttaaaggata
     1021 cgaagctctt gagtttggtg taagtctttc gtcatcaaaa tcaaattctc catttggata
     1081 tatcattgca ttttcaacta ttacaccatg gttcaagtaa tcgtctttat gcattactgc
     1141 ttcaaaaata tctggttctt ttgctggatt aatatttatc atttttgcat agcaaccatt
     1201 ttcgaagtta gcaactcttt tattactcca gccatgttcg tcatcgccta ataaagctct
     1261 gcgagggtca gccgacaaag tagtttttcc tgttcccgac agacctaaaa tcaaagctgt
     1321 ttttccgtct ttaccttcgt ttgcagagca atgcaatggc aacacatctt ggaaaggtaa
     1381 ataataattc ataactgtaa acatagtttt tttaaccgag cctaaataag ctgaacctat
     1441 gataattccg atacgattat caaaatctat taaaactatt ttatctgtag tttttccgtt
     1501 tggcaactta cgtaatttcc attcatattt tttctcatct aatttatcga atggcagtga
     1561 tattaaataa aaaggttttt ctgcaaaaca actgttattc aaatcttttg gaacaggacg
     1621 gaacatattg tcgccaaaaa cagaagttaa agcttgagga gtgataagtt taactggtaa
     1681 ggcgtactca gaatctgcac caataactct gtcgtttata taaatacgtt cgtattttcg
     1741 cattttttcc attgcatctt gaaaaatttc ttcaaacaaa gattctgttg ctggcaaatt
     1801 gtttggagaa ttccaatcaa tatgaatttg actttgtgga cgttttacga acaatgtgtc
     1861 tttagggcta cgtcctgtag aatcgggagc tgtccaagtt gcaagatttc cgacttttga
     1921 aactatacat tcgtgatatt ttaccgcatc accgattaaa gtttttctat ccgcatttac
     1981 tatcagcgtt ttacactctt taagatttgc atcaaccttg ttgtgtattt ctttaaaatt
     2041 taatgtactc ataaataact ttttttcttt gtgcaaaaat actacttata ttttaataaa
     2101 tggtataaaa cataaaataa aatgtattta tcataaaata tttaactata agtagtttta
     2161 aagacataaa acattttttc ttgtaaaatt tatttataac tttgttttcg attatcgcaa
     2221 gttaaatttt atgaatatac aaattaacaa taagtctttt caaatttttt caggagcgaa
     2281 agtaattgat gctgtaagaa tgtattttac agagattggt aaggactttg aaatagataa
     2341 aattgtggta gaagataaat ttggtaacag acttgaattt gatggtcgtt taagaaataa
     2401 caaatcatta tttattaaat tttaaaacta tgaatattaa aaaaacttac agattatttt
     2461 tcagcattgc aattatttta gcaatatttc aaacttattc gtgcaaacct aaggtagaag
     2521 aagaaaataa aaaagtggta attcttgcag taaatgacat gcatgctaat attgacaatt
     2581 tcccacgttt tgcgtttatt gttgatagct taagagaaat ttatcccgat ttattattag
     2641 tttctgctgg tgacaatcaa agtggaaatc ctgcaaacga ccaatatcca caaaaaggaa
     2701 tgccaataac agaacttatg aatatgatta agtttgatgt atgtgctgtc ggaaatcacg
     2761 agtttgacac aaaacaagat ggtttcaaat acattattga taattcaaaa tttgattata
     2821 ttagtgcaaa cataaaacct agctcaaatt taggactaaa tattaagcct tttaaaacta
     2881 taaaaatgcc taacggacta aaaattgctt ttacttcggt aatttccttg aacgaaaatg
     2941 gcattccaga ttctcatcca aataatttaa cagatataaa atttaccgat ccttttacaa
     3001 cagcgaaaga ttatctttat ttaaaagatg agcatgattt attaattttt gtaaaccatt
     3061 ttggttttga agaagatgtt gaattagcag aacaattccc aaaaggaaaa gttgatttaa
     3121 taattggcgg acattcccat actgttgttg aaaaagaaca aatttttaat gatattttaa
     3181 ttactcaagc tggaaataaa ttaaaatatt gcactcttat tgagcttgaa gtagaaaaag
     3241 attattctgt aaaaaaatcc atgaagctta ttcctataaa taaaaatgga gaaatcattg
     3301 cagagtttca agaaaaagtt gatgaatata acgacagtcc attcttaaac gaaaaaattg
     3361 ctgaagctga agatgatttt acctcatacg aacaagttgg atatctaatg ggagatgctt
     3421 taaggttaaa tctcaacaca gatatagctg ttgtaaatcc tggcggtgta agaatcgaca
     3481 aactcgacaa aggcgacatt aacgtcagag acgtatattc aatggatcct tttggaaatg
     3541 aagctgtgat ttttaatctg acaggaaacg aaataaaagc aatgtattta tcagccttta
     3601 caatagatga atacttacct atctaccctt ctggaataaa agtaaaatat attttgaaag
     3661 ataaaaaaac aattaaagac gtaatatttt acacagaatc aggcgaagat ttagatatgg
     3721 aaaaaacata ttctgttgct atgaataact atatggcatc tgtttataat tatgaacatg
     3781 ctgattcagg aacaggacaa tttgttacta ctgccgaaaa catgattgat tacttaaaaa
     3841 acttagaaac tgttccaagc tataaaaatg aaaaaagagt agaattaaaa taaacgttaa
     3901 tgttaaaaac tgttaaattg agcttactct caaataaatt ttttaatttt acgtcaaatt
     3961 ataaacttat aaatttttaa aatatgagaa aaacaacaat tttattatta tcaagtatta
     4021 ttttatttac tatgtcgtgt aaacaaaaaa ccaatgaagt aagcgataat cctttgcttc
     4081 aagaatggga tacgccttat ggtgttccac ctttcgataa aataaaaatc gaacattaca
     4141 aacctgctct cgaagctgca atgcaagagc aaaaggataa tatccaaaaa attattgaca
     4201 acaaagaagc tccaaatttt gaaaacacaa tcgctgcctt agaatatagt ggtttaactt
     4261 tatcaaaagt agctggagta tttttcaatt tattatcttc tgatacaaat gaagaacttc
     4321 aaaaaattgc tcaagaaatt tcgccaaaac tttcaaacca cagcgatgaa attataatga
     4381 atcctgagct ttttgctaaa attgaggaag tttataagaa taaagataat agcaacttaa
     4441 acaaagaaca gctaagagtt ttagaattaa cttacaggga ttttgttaga agtggtgcta
     4501 aacttaacga tgccgacaaa aaacgtttaa tggaaattaa cgaaagattg tctttattag
     4561 ctcttcaatt tggcgataat ttattagcag aaacaaacgg ctacaaactt attattgata
     4621 aagaagaaga cttagccggt ttgcctgaag gacttagaga agctgctgca attactgcaa
     4681 aagaaaatgg cgaagaagga aaatggatgt tcactttaca caatgctagt gttatgccat
     4741 ttttacaata tgccgacaat cgtgaattaa gaaaagacat tcaacaagct tatataaatc
     4801 gttgtaacaa caacaacgaa tatgacaaca aaaaaatcat tctcgaatta gttgagctaa
     4861 gaatagaaag agctaaactt ttgggatatg aaaatcattc tcaatatatt ttagatgaca
     4921 atatggcaaa aactccagaa gcagtttatg aacttttaaa ccaactttgg acaccagccc
     4981 ttgaacttgc taaagaagaa gctgaacttt acagagaaat gatgaaaaga gatggtattc
     5041 aagatgattt acaagcttgg gactggagat attatactga aaaagtaaga aaagaaaaat
     5101 ataatttaga cgaagaagaa ctaaaaccat attttaaatt agaaaatgtt agagatggtg
     5161 tttttgatgt ttcaaacaaa ttgtttggtc taagtttcgt aaaatctaat gaaattccta
     5221 tttatcatcc agacgttgaa gcttacgaag ttaaagatga aaataatgaa gtagtagctg
     5281 ttttatatat ggattatttc ccacgtccaa gtaaaagcgg tggtgcatgg atgaacgatt
     5341 atcgtggaca atatgtttat gaaggtaaaa atgtaattcc aatcattaca attaattgta
     5401 acttcacaaa accaacaagc actgctcctt ccctattaac ttttgacgaa gcacaaactc
     5461 ttttccacga atttggacat ggcttacatg gaatgctttc aaaatgtact tatccttcaa
     5521 taagtggaac aaacgtacct cgcgattttg ttgagcttcc ttcacaacta atggaacatt
     5581 gggcatcaga acctgaagta atgaaatctt atgctcgtca ttatcaaact aatgagccaa
     5641 ttccagatga attaatcaaa aaattagaag atgcagaaca tttcaatcaa ggatttgcta
     5701 ctactgagtt tttagctgct gctcttttag atttatatta tcacaatcta accgaaatga
     5761 attttagtga tgtattagtt gccgaaaaag aagctctaga agctattggt ctaatacctg
     5821 aaatagttgt cagatatcgt agtccatatt tctcacatat ttttgcaggc ggatattcaa
     5881 gcggatatta tagctatatc tggagcgaag ttcttgattc tgacgcattt gctttattta
     5941 aagaaaaagg aattttcgac cctgcaactg caaaatctct aaaagataat atctactcaa
     6001 atggtcataa agatgatcct atgacaatgt atgttaactt tagaggcaga gaaccaaaaa
     6061 ttgacgcttt gcttgaaggc agaggtttaa aataaaaata ttaaataaaa aaaacgaggc
     6121 tgtctcaaaa caaattttaa aacaatgaat tttcaaggtg tttcagaagt
//
LOCUS       DAOPNX010000120         6068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_14249_length_6068_cov_8.143855, whole genome shotgun sequence.
ACCESSION   DAOPNX010000120 DAOPNX010000000
VERSION     DAOPNX010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14249_length_6068_cov_8.143855"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..934
                     /locus_tag="PKN53_10125"
     CDS             89..934
                     /locus_tag="PKN53_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD89345.1"
                     /translation="MKIFILDTPEFKIDGGAYFGVVPKIIWQKKYPCDENNMCPAACR
                     SILVDRGDRVILFDTGIGEKPEEETNDLYFTDYSQNLKENLKKIGYLPEQITDVVLTH
                     LHFDHCGGNTIIDKKSGKRIIAFPNAKYYVSKDQFQNAIKPNYRERSSFLPENWDIIE
                     KENALIIVEKDFFLCPEIELKLFYGHTPGLMLPIIRTNNKTIFFTGDLIPARASIPLA
                     WISAYDLFPLTSIEEKQEILNQAADENWLLVFQHDYFCEACYVEKTERGVRAKEAIKF
                     SDIKL"
     gene            950..1324
                     /locus_tag="PKN53_10130"
     CDS             950..1324
                     /locus_tag="PKN53_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89346.1"
                     /translation="MKSIVKFVFITCLTAVLLTACEKDYKYERSIKLLTKNIWQIKTY
                     VNYAENQTSITRNAKFYFKENNELVKIYDNGEIATTKWELCQDADFLLIGNNMFKITV
                     LNRRVMSLRHGDAELFFVPIKE"
     gene            1326..2306
                     /locus_tag="PKN53_10135"
     CDS             1326..2306
                     /locus_tag="PKN53_10135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOD89347.1"
                     /translation="MEENQIKFLIIRFSSIGDIVLTTPVIRNLKNQVDGAVIHYLTKP
                     QYSSILENNPYVDKVHVLKSYKETIRELKDEGFDYIIDLHNNLRTWRFKNRLKILDFS
                     FPKLNWEKYQMVRFKKNKLPDIHIVDRYLKTVYLFDVDNDNKGLDYFIPESDKINISD
                     FLKTENTEFIAYAIGGQHFTKKLPVNKISELCSKINKPIILLGGKEDFENGEKIAQEN
                     SHCINLCGKLNLNQSASIVEQAYFVISHDTGLMHIAAAFKKNIASIWGNTIPEFGMYP
                     YQAGENCKIFEIKDLKCRPCSKIGFKKCPKKHFDCMLKQNLDEIALFLNA"
     gene            2377..3138
                     /locus_tag="PKN53_10140"
     CDS             2377..3138
                     /locus_tag="PKN53_10140"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE N-terminal
                     domain-containing protein"
                     /protein_id="HOD89348.1"
                     /translation="MKTINFFIGLCVIFSLVACNNKNADFIDENSAKNTIDWAGTYWG
                     IIPCADCPGIETKIKINNDESFVLTTNYLEVENSENIIEGKFTWTDNGNSIELSADSE
                     NENPTILKIEENQIKYLNLDGTEIEGELADNYILAKNGNPLVDNKKWKITEIYGQKLE
                     SNEDELFMNFNAEDSKVYAKVDCNLMNWEYEIHNNSKIEFSQLTTTLMFCPDSLEDQF
                     IEAIKQADNISTDGKTLSINNADMLNLLELELIKE"
     gene            3195..4211
                     /locus_tag="PKN53_10145"
     CDS             3195..4211
                     /locus_tag="PKN53_10145"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HOD89349.1"
                     /translation="MSFNIKNIAKKIKQIQAKTGLDSFVIGIIGVIILAYFWWQPGVY
                     EGKVTLNDIGNIGVSLIFFFYGLKLSPQELKKGLSNWKLHIVVQTATFIIFPIVVLVF
                     MAIFGSFGNKLLWLGIFYLAALPSTVSSSVVMTSLARGNITSAIFNASLSSIIGVFIT
                     PVWMGIFISTSGGDFDLTNVIIKLSLQVLLPVAIGVLLHPKFGKIALKNSNKLRIFDQ
                     SIILLIVYVSFCDSFANKMFSGYATWELIVLGLAMIALFFIIYGIVTFIGKLLKFSIE
                     DHITAVFCGSKKSLVHGTVMSKVLFPGIAGVGVILLPLMIFHALQLVLASMLAQKFAK
                     RPDK"
     gene            complement(4192..4830)
                     /locus_tag="PKN53_10150"
     CDS             complement(4192..4830)
                     /locus_tag="PKN53_10150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD89350.1"
                     /translation="MFCPLCNNVNTEVFQEVNSKLYYKCYNCDLIFLDKAHYLNQEEE
                     KKRYNFHQNSLENQGYIDFLSQLINPLKEFLKPTFQGLDFGCGPNSVLAQLMNQYGFS
                     CDFYDPYFFPKLVKNKTYNFITATECFEHFFEPKKEIELLISLLKPNSYLGIMTELLA
                     DESNFKNWYYIKDPSHVCFYSLKSFEFLCKKYGFKMLYTDKKRVVILIYQGV"
     gene            4936..5988
                     /gene="rfbB"
                     /locus_tag="PKN53_10155"
     CDS             4936..5988
                     /gene="rfbB"
                     /locus_tag="PKN53_10155"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOD89351.1"
                     /translation="MNKTIFITGGAGFIGSHVVRLFVTKYPNYNIVNIDSLTYAGNLE
                     NLRDIEKLPNYKFEKIDIVDEQAINAVFNKYNPDGVIHLAAESHVDRSISNPTEFIFT
                     NIIGTVNLLNASRACWKDNFSGKRFYHISTDEVYGSLGAEGFFTEETSYDPRSPYSAS
                     KASSDHLVRAYFHTFDLPVVLSNCSNNYGPNQFPEKLIPLAINNIKNMKPIPIYGKGE
                     NVRDWLYVHDHARAIDIIFHKGKLGDTYNIGGNNEWQNIDLIKVLCKVMDKKLGREEG
                     KSASLITFVKDRAGHDLRYAIDSSKLMKELGWEPSLQFEEGLEKTVDWYLQNEEWLNR
                     IVSGDYKSYYEKMYGK"
BASE COUNT         2228 a          829 c          944 g         2067 t
ORIGIN      
        1 tacaaacaat tttttaatta tatttgcttc ttattttatt taaaagaaaa agtattttca
       61 cttttaaata ttgtaaaaag tgtaaactat gaagattttt atattagata caccagagtt
      121 taaaattgac ggtggagcgt attttggcgt tgttccaaaa attatttggc aaaaaaaata
      181 tccctgcgac gaaaataata tgtgtcctgc tgcatgtcgc tcaatacttg tcgatagagg
      241 tgatagagtg attttattcg acactggcat tggcgaaaag cccgaagaag aaactaatga
      301 cttgtatttt actgattata gccaaaattt aaaagaaaat ttaaaaaaaa ttggctacct
      361 accagaacaa ataactgatg ttgtattaac tcatctacat tttgaccact gcggaggaaa
      421 tacaataatt gataaaaaaa gcggaaaaag aattatagct tttccaaacg ctaagtatta
      481 tgtttcaaaa gaccaatttc aaaatgcaat aaaaccaaat tatagagaac gctcttcatt
      541 tttgcccgaa aattgggata ttatcgaaaa agaaaatgct ttaattattg ttgaaaaaga
      601 tttttttcta tgtccagaaa ttgaactaaa attattttac ggacacactc caggacttat
      661 gcttccaatt ataagaacta ataacaaaac catatttttt acaggagatt taattccagc
      721 tagagcatca attccattgg cttggatttc ggcttatgat ttgtttccgc taacaagcat
      781 agaagaaaaa caagaaattt taaaccaagc tgcagacgaa aattggcttt tagtttttca
      841 gcacgattat ttttgcgagg cttgctatgt ggaaaaaacc gaacgcggag taagagcaaa
      901 agaagcaata aagttttcag atattaaatt ataagttttt aaaatattta tgaagtctat
      961 agtcaaattt gtttttatta cttgcttaac agcagtactg ctaacagctt gcgaaaaaga
     1021 ctataaatat gaacgtagta tcaaattgct aaccaaaaat atttggcaaa taaaaactta
     1081 tgtaaattac gccgaaaacc aaacctcaat tactagaaat gcaaaattct attttaagga
     1141 aaataatgag cttgtaaaaa tatacgacaa tggcgaaatt gcaacaacaa aatgggaact
     1201 ttgccaagat gctgattttt tattgattgg aaataatatg tttaaaatta cagtgttaaa
     1261 tagaagagta atgtcgttgc gacatggtga tgctgaactg ttttttgtcc ctataaaaga
     1321 ataatatgga agaaaatcaa ataaaatttt taataataag attttcaagt attggcgata
     1381 ttgtacttac aacacctgtt ataagaaatt taaaaaatca agtcgatggt gctgttattc
     1441 actaccttac aaaaccacaa tacagttcaa ttttagaaaa taatccttat gtcgataaag
     1501 ttcatgttct taaatcttat aaagaaacta ttcgtgaatt aaaagacgaa ggttttgatt
     1561 atattattga tttacacaac aatttaagaa cttggcgttt taaaaatcga ctaaaaattt
     1621 tagactttag ttttcctaaa ttaaattggg aaaaatatca aatggttagg ttcaaaaaga
     1681 acaaactccc tgatattcat atagttgaca gatatttaaa aactgtttat ttgtttgacg
     1741 tagataatga caacaaaggt ttagattatt ttattccaga aagcgataaa ataaatattt
     1801 cagatttttt aaaaactgaa aacacagaat ttattgctta tgccattgga ggacaacatt
     1861 ttacaaaaaa acttccagta aataaaattt ctgaattatg ttcaaaaatt aataaaccaa
     1921 ttattttact tggcggaaaa gaagattttg aaaatggaga aaaaattgct caagaaaact
     1981 ctcattgtat aaatttatgt ggcaaactaa atctaaatca atctgcttct attgttgagc
     2041 aagcatattt tgtaatttcg cacgacacag gtttaatgca cattgcagca gcttttaaga
     2101 aaaatatagc ctctatttgg ggcaatacaa ttccagaatt tggaatgtat ccttaccaag
     2161 caggagaaaa ttgtaaaata tttgaaatca aagacttaaa atgccgacct tgctcaaaaa
     2221 ttggatttaa aaaatgtcca aaaaaacatt ttgattgcat gttgaaacaa aatcttgacg
     2281 aaattgctct attccttaat gcttaacgat tttatgttaa taaacataaa acattttggc
     2341 tcgttttttg ttgttaatta accataaata ttagatatga aaaccataaa tttttttata
     2401 ggattatgcg ttatttttag tctagtcgca tgtaataaca aaaatgcaga ttttattgat
     2461 gaaaattctg caaaaaacac cattgattgg gctggcacat attggggaat tattccttgt
     2521 gctgattgtc ctggaattga aacaaaaatt aaaatcaata acgatgaaag ttttgttctg
     2581 acaacaaatt acttagaagt agaaaattca gaaaatatta ttgaaggaaa atttacttgg
     2641 acagacaatg gaaacagcat agagctaagt gctgattctg aaaacgaaaa cccaacaatt
     2701 ttaaaaattg aagaaaatca aatcaaatat ttaaacttag acggcacaga aatcgaaggt
     2761 gaacttgctg ataattacat tcttgcaaaa aatggaaacc ctttggttga taataaaaag
     2821 tggaagatta cagaaattta tggacaaaaa cttgaaagta atgaagatga attgtttatg
     2881 aatttcaatg ccgaagattc taaggtttat gctaaagttg attgcaactt aatgaattgg
     2941 gaatacgaaa tacataataa ctcaaaaatt gaattttcac aactaacaac aactttaatg
     3001 ttttgcccag acagtttaga agaccaattt atagaggcaa taaaacaagc cgataatatt
     3061 tctactgacg gaaaaacttt aagcataaac aatgccgata tgcttaattt gcttgagtta
     3121 gagctaataa aagaataatc attttcctgc taaacattca gttttttatt taagtttgca
     3181 caaatttatt tttaatgagt ttcaatatta aaaatattgc aaaaaaaata aagcaaattc
     3241 aagcaaaaac aggcttggat tcttttgtta ttggaattat tggagtaatt attctagcgt
     3301 atttttggtg gcaacctggc gtttatgaag gaaaagttac tttaaacgat attggaaata
     3361 ttggtgtttc tttaattttt ttcttctatg gcttaaaatt aagtccgcaa gaactgaaaa
     3421 aaggcttgtc gaactggaaa ttacatattg ttgtgcaaac tgctactttt ataatttttc
     3481 ctattgtagt tttagtgttt atggcaattt ttggctcttt tggaaataaa cttttatggt
     3541 tgggaatttt ctatttagca gcattaccgt caaccgtttc ttcgtctgtt gttatgacat
     3601 ctcttgctag aggaaatatt acttctgcaa tttttaatgc ttctctttca agtataattg
     3661 gagtttttat tactcctgtt tggatgggaa tttttataag cactagcggt ggtgattttg
     3721 accttactaa cgttattata aagctatctt tacaagtttt attacctgtg gctattggag
     3781 tgcttttaca cccaaaattt ggaaaaatag ccttaaaaaa cagcaataaa cttagaatat
     3841 ttgaccaaag cattattctt ttaattgttt atgtttcttt ttgcgattca tttgcaaata
     3901 aaatgttttc gggatatgca acttgggaat taattgtgtt aggattagca atgatagcct
     3961 tattttttat tatttatgga attgttactt ttatcggaaa acttttaaaa ttctcaatag
     4021 aagaccatat tacagctgtt ttttgtggtt ctaaaaaatc tttagttcac ggaacagtaa
     4081 tgtcaaaagt tcttttccca ggaattgctg gtgttggtgt tattttattg cctttaatga
     4141 tatttcatgc tttgcagtta gttttagcaa gtatgttggc tcaaaaattt gctaaacgcc
     4201 ctgataaata agaattacca cccttttttt gtctgtataa agcattttaa aaccatattt
     4261 tttacataaa aattcaaaac tttttaaact gtaaaaacaa acatgagagg gatctttaat
     4321 gtaataccag tttttgaaat tgctttcgtc tgcaagcaat tctgtcataa ttcctaaata
     4381 agaattaggt tttaataatg aaattaataa ttcaatttcc ttttttggtt caaaaaaatg
     4441 ctcaaaacat tcggtagctg ttataaaatt ataagttttg tttttaacta atttggggaa
     4501 aaaatagggg tcgtaaaaat cgcaactaaa gccatattga ttcattaatt gtgctaaaac
     4561 ggaatttggt ccacagccaa aatccaatcc ttgaaatgta ggttttaaaa attcttttaa
     4621 aggatttata agttgactta aaaagtcgat atatccttga ttttcaagtg agttttgatg
     4681 aaaattgtaa cgcttttttt cctcttcctg attcaaatag tgagctttgt ctaaaaatat
     4741 caaatcacaa ttataacatt tataataaag ttttgaattt acttcttgaa aaacttctgt
     4801 attaacatta ttgcaaagtg gacaaaacat agtaaagcct ttttaattta caaaattaag
     4861 attttttata aatttttagt aagcctcact aaatatttgt atcttagcga attaaatttt
     4921 aaaaaaattt ttactatgaa caaaactatt tttattactg gtggtgctgg ctttattggc
     4981 tctcatgttg ttagactttt tgtaacaaaa tatcctaact acaatattgt aaatattgat
     5041 agtttaactt atgctggaaa tttagaaaac cttagagata ttgaaaaatt accaaattat
     5101 aaatttgaaa aaatagatat agttgacgag caagctataa atgctgtttt taataaatac
     5161 aatcccgatg gtgtaattca cctagcagca gaatctcatg ttgaccgcag tattagtaat
     5221 ccaaccgaat ttattttcac aaatattata ggaactgtaa acttattaaa tgcttctcgt
     5281 gcttgttgga aggataattt ttctggaaaa cgtttttacc atatttctac cgatgaagtt
     5341 tatggttcgc ttggtgcaga aggttttttc acagaagaaa caagctatga tccacgttcg
     5401 ccatattctg cttctaaagc aagttcagac catttggtaa gagcatattt tcacactttt
     5461 gatttacctg ttgttttgtc taattgttca aacaattacg gtcctaatca atttcctgaa
     5521 aaactaattc ctttagcaat taacaatatt aaaaacatga aaccaattcc aatttatgga
     5581 aaaggcgaaa atgttcgcga ctggctttat gttcacgacc acgcaagagc tatagatata
     5641 attttccata aaggaaaact tggcgatact tacaacattg gcggaaataa tgagtggcaa
     5701 aatattgatt tgataaaagt actttgcaaa gttatggaca aaaaacttgg cagagaagaa
     5761 ggaaaatcag cttcgctaat cacttttgtt aaagaccgtg caggacatga tttgcgttat
     5821 gcaatcgata gctcaaaact aatgaaagaa cttggctggg aaccatcttt acaatttgaa
     5881 gaaggactag aaaaaactgt tgattggtat ttacaaaatg aagaatggct aaatcgcata
     5941 gtatcaggcg attacaaaag ctattacgaa aaaatgtacg ggaaataatt cagttaaaag
     6001 ttaaaagttg aaagtcgaag cgactttaca aactttacaa actttacgaa ctttataaac
     6061 ttttaact
//
LOCUS       DAOPNX010000121         6050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_14313_length_6050_cov_4.580317, whole genome shotgun sequence.
ACCESSION   DAOPNX010000121 DAOPNX010000000
VERSION     DAOPNX010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14313_length_6050_cov_4.580317"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1562
                     /locus_tag="PKN53_10160"
     CDS             <1..1562
                     /locus_tag="PKN53_10160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HOD89352.1"
                     /translation="QEQVEEKKPVEKPQDPFAPSAIKAKAKAKLKEKGIGEPMAVSFV
                     AALLSFPILGLGPVNVGNREYYMNLVRGKETKFNNLFDGFKKENVVRNLILGLFKTIY
                     LAFWGVLFLIPAIFKWYSYCLAPYLVIDRPELTGNGSINMSKAVMFGSYRLRLFKLHL
                     SFLGWLALSVLTGGILLLWVIPYIQTAEAYFYEEVSKIEFPERAKIIRHADSKDDREV
                     LLEVQDVDIYFKVGKFTVKACNHLNFKVYKGETFGLVGESGSGKTTITRAIIGINELT
                     HGRILFKGQDISRIKDKEGRQRLKKNIQMIFQDPTASLNERANIDYIVSEGLYNFHLF
                     ENEEDRHNKVIKALKDVGLLPEHLSRYPHEFSGGQKQRIGIARALIVEPELVLADEPI
                     SSLDVSIRAQVLNLLRDLQEQNNLTYIFIAHDLSIIKYISDRIAVMHQGYVVELGTAE
                     SIYSNPVHPYTKSLLTAIPQPDPASKDDRVKVIYNKGNMDYEKMDWIEVVPEHFVLGT
                     PELIKKWTKKK"
     gene            1698..1994
                     /locus_tag="PKN53_10165"
     CDS             1698..1994
                     /locus_tag="PKN53_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89353.1"
                     /translation="MVLVIMPLAIGIPLFLANRDRPEFLEIPLAIFGVFELLVLTVRI
                     QVRDNKKRKAGNLKEDKGSEEYQSHVNFRKIILISAFINLALSLVAFWIFGRGV"
     gene            2032..4035
                     /locus_tag="PKN53_10170"
     CDS             2032..4035
                     /locus_tag="PKN53_10170"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOD89354.1"
                     /translation="MHFAGYEQEPNFILYFINFNQEKIKEITLEFFGKQQTYPINSSE
                     SKITLVLDEDVNYIPKLFSVTTDKVNYLKDYPDYDTVTSPKAIHSLGPEKELYRRELK
                     KKFNYNNVKCVYEESVKYWHCVCGHTNFSIDDKCEACGKEKSKLAEVHPSYASQAATR
                     HQENQILSLFLALYITLYLIDIFVQMFTGDFLFTNYDKNTLFAVTNRFIVPVLIITVV
                     ALKMVFNNKHIKVATDILKILFFLLVLYLNILPIISFIGTAYNLTFLWSVDILIAILV
                     IRSLVTVGQKVYNFSLLGTIIVFFFMSIFLYGVYKDSKITIHPEGVSLTVQEEGDQKK
                     YEMPETLNNMKVFSVIFSYNKEYEMEELVVSNNASIVAFTSTATFKNLKVVEVKENNK
                     VYYADNNVLYKDEKIILVPLAITEVTLNSDVVNSKAFYNHYNLKSVTILENVKVIEKS
                     AFANATNLENINFAPNSSLKTIEEYAFKNTGIKKIEIPISVENIEIGILDGCYALESY
                     KTPFIGKKRENHDQFYSSSDIFVYHFGSVIHSQSEKIPQSLKHIEVYDIDRIHNTTFY
                     NVKSVETIVLPDELLYMGKSSFYGCESLLEFTIPNGVPDIKEECFKYCSSLKTIFIPA
                     SVTNIEKNAFQYCNELTTVIYLGDKTNLTIDPIGNQKLIDLLN"
     gene            4094..4531
                     /locus_tag="PKN53_10175"
     CDS             4094..4531
                     /locus_tag="PKN53_10175"
                     /inference="COORDINATES: protein motif:HMM:NF002459.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HOD89355.1"
                     /translation="MIQLYISPSCSSCRKVKKYFDNHGIPYMEKNILNAPISKEEIMR
                     MLTKSENGFEDIISTRSNVFKEKGNKIEQMKTSELVDFIIDNPSVLKRPIIVADYELQ
                     VGYNEEDITLFLPQDKREAICSVCLKNNPEDCDYIKHLRFSDK"
     gene            complement(4558..5061)
                     /locus_tag="PKN53_10180"
     CDS             complement(4558..5061)
                     /locus_tag="PKN53_10180"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOD89356.1"
                     /translation="MSNFSNPDFQTNHYFDTTRFSLKAEDCSLRVRERETLELVFKIK
                     KSYETVTFSDIITKEQLENMIETGDIPKGDVKPEVKKIIKDQKLVNFMSLSTIRLFMQ
                     CKNGVVYLDKSTYCGITDYELYYNPTTNYYQGRKEFVELIRDFNIVYKKCEKKIIRTY
                     EALKRFN"
     gene            5214..5897
                     /locus_tag="PKN53_10185"
     CDS             5214..5897
                     /locus_tag="PKN53_10185"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HOD89357.1"
                     /translation="MIFLNSNDWQSFLLPYELAVEGFIVKLESIRKQYILKGLYNPIE
                     IVTGRVKTVEAILQKATRLEIESSDIPEKIQDIAGVRIICKYIDDVYLVQDLITSRRD
                     LKIITIKDYVKKPKPSGYQSLHIIAQYVTETIEGPNTIYIEFQVRTHVMHFWASIEHS
                     LNYKYDYQIPGTIKFRLLKASQAAQKLDVEMTKIKKTIDKLEEKHELKKAKNPLEEKE
                     YKLNSVRKE"
BASE COUNT         2229 a          768 c          975 g         2078 t
ORIGIN      
        1 ttcaagaaca agttgaagag aaaaaaccag ttgaaaaacc tcaagatcct tttgcgcctt
       61 cagctataaa agccaaagct aaagcaaaac taaaagaaaa aggtattggc gaaccaatgg
      121 ctgtttcttt tgtagcagcg ttattaagtt ttccaatttt agggttaggc ccagttaatg
      181 ttgggaatcg tgaatattat atgaatttag ttcgaggtaa agaaactaaa ttcaataatt
      241 tatttgatgg ttttaaaaaa gaaaacgtag ttcgtaactt aatactaggt ttattcaaaa
      301 ctatttattt agcattctgg ggagttttat ttttaatccc agctatcttt aaatggtatt
      361 catactgttt agctccttat ttagtaattg atcgtcctga attaacaggc aacggatcta
      421 ttaatatgag taaagcagtt atgtttggct cttatcgcct acgtttattt aagcttcact
      481 tgagttttct tggttggctt gcgttaagcg ttttaactgg aggaatacta ctattatggg
      541 taattcctta tattcaaact gcagaggctt atttctatga agaagtttct aaaattgaat
      601 ttccagaaag agctaaaatt attagacacg cagattcaaa agatgatcgc gaagttttac
      661 tagaagttca agatgttgat atctatttca aagtgggaaa atttactgtg aaagcatgta
      721 accacttaaa tttcaaagtg tacaaagggg aaacatttgg cttagtaggg gaatcaggtt
      781 cgggtaaaac aacaattact agagcaatta taggtattaa cgaattaact catggtcgta
      841 tcttattcaa aggtcaagat atttcgcgta ttaaagataa agaaggaaga caaagattga
      901 agaaaaacat tcaaatgatt ttccaagatc caactgcttc tttaaacgaa agagcaaata
      961 ttgactatat cgtttctgaa ggtttatata acttccactt atttgaaaac gaagaagatc
     1021 gccataataa agtaattaag gcattaaaag atgtaggatt attaccagag cacttgtcac
     1081 gttatccaca tgagtttagt ggaggtcaaa aacaaagaat tggtattgct agagctttga
     1141 ttgttgaacc agaattagtt ttagctgatg aacctatttc ttcacttgac gtttcaatta
     1201 gagctcaagt attgaatcta ttaagagact tacaagaaca aaataactta acatatattt
     1261 tcattgctca tgacttatca attattaagt acatttctga tagaattgcg gtaatgcatc
     1321 aaggatatgt tgtggaactt ggaacagcgg aatctattta ttcaaaccca gttcacccat
     1381 atactaaatc attattaact gcaattcctc aaccagatcc agcaagtaaa gatgatcgtg
     1441 tgaaagttat ttataataag ggtaatatgg attacgaaaa aatggattgg attgaagtag
     1501 ttcctgaaca cttcgttttg ggtacaccag aactaattaa aaagtggact aagaaaaaat
     1561 aatcctaagt ttataaaaaa gcattcgttt tcgaatgctt ttttccttta atattttgat
     1621 gaaatgttgt ataatataaa acataagagg taaagaatat gaatacaaat aaaacaataa
     1681 cacaaattgg aattatattg gtacttgtaa taatgccatt agctataggt attcctttat
     1741 ttttagcaaa tagagataga ccggaatttc tggaaattcc tcttgctatt tttggagtat
     1801 ttgaactttt ggtacttact gtaagaatac aagtccgtga taataaaaaa agaaaggctg
     1861 gaaatcttaa agaagataaa ggtagtgagg aatatcaaag tcatgtaaac tttagaaaaa
     1921 ttattcttat ttcagcattt ataaacttag cattatcttt ggttgctttt tggatttttg
     1981 gtagaggcgt gtagtatgca ttatacaaaa agaatataca aaaattttcc tttgcatttt
     2041 gcaggttatg aacaagaacc taactttatt ttatatttta taaattttaa tcaagaaaaa
     2101 attaaagaaa ttactttaga gttttttggg aaacaacaaa cttatcctat taattctagt
     2161 gaatctaaga ttactttggt tttagatgaa gatgttaatt atattccaaa attattttct
     2221 gttaccactg acaaggttaa ttatttaaaa gattatccag actatgatac agttactagt
     2281 cctaaagcta ttcattcttt agggccagaa aaagaattgt atcgcagaga attaaaaaag
     2341 aaattcaatt ataataatgt taagtgtgtt tatgaagaaa gtgtgaaata ttggcattgt
     2401 gtttgtgggc ataccaattt tagtattgat gataaatgcg aagcatgtgg caaagaaaaa
     2461 agcaaattag cagaagtaca tccttcatat gctagtcaag ctgcaacccg tcaccaagaa
     2521 aatcaaattt tatcactttt tctagcactt tatattacac tatatttaat agatattttt
     2581 gttcagatgt ttacaggcga tttcttgttt acaaactatg acaagaatac attatttgct
     2641 gttacgaatc gctttatagt gccagtttta attataactg ttgtggcatt gaaaatggta
     2701 tttaataata aacacattaa agttgcaacc gacattttaa aaattttatt tttcctttta
     2761 gtgctctatt taaatatctt accaataatt agttttattg gaactgctta taatttgacg
     2821 tttttatggt cagtagatat cttaattgca attttagtta ttcgttcgct tgttacagta
     2881 ggacaaaaag tgtataattt ttctctttta ggcacaatta tagttttctt ttttatgagc
     2941 atcttccttt atggagttta taaagattcg aaaattacaa ttcatccaga aggagtgtca
     3001 ctaacagttc aagaagaggg tgatcaaaaa aaatatgaaa tgccagaaac actaaataat
     3061 atgaaagttt ttagtgtgat tttttcttat aataaagagt atgaaatgga agaattggta
     3121 gttagtaata atgctagtat agtagctttt acatctacag ctacatttaa aaacttgaaa
     3181 gttgtggaag ttaaagaaaa taataaagtt tattatgccg ataataatgt cttatataaa
     3241 gatgaaaaaa taattttagt tccacttgca attacagagg taaccttaaa tagcgatgtt
     3301 gtaaattcaa aggcatttta taatcactat aatttaaaat cagtaactat tttagaaaat
     3361 gtaaaagtaa ttgaaaaaag tgcatttgca aatgcgacta atttagagaa tataaatttt
     3421 gcacctaata gtagtttaaa gacgatcgaa gaatatgctt ttaaaaatac aggtattaaa
     3481 aaaattgaaa ttcctatttc tgtagaaaat attgaaattg gaattttaga tgggtgttat
     3541 gcgcttgagt cttataaaac accatttatt ggaaaaaaac gcgaaaacca tgatcaattt
     3601 tattcttcat cagatatctt tgtatatcat tttggttcag ttattcatag tcaatcagaa
     3661 aaaattcctc aaagtttgaa acatattgaa gtatatgata ttgaccgaat tcataataca
     3721 actttctata acgtaaagag tgtagaaaca atcgttttac ctgacgagct tttatatatg
     3781 ggaaaaagtt ctttctatgg ttgtgaaagt ttactagaat ttacaattcc aaatggagta
     3841 ccagatataa aggaagagtg ctttaaatat tgttctagtt taaaaacaat ctttattcca
     3901 gcttcggtta caaatattga gaagaatgct tttcaatatt gtaatgaatt aacaactgtc
     3961 atttatttag gtgataaaac taatttaact attgacccaa taggcaatca aaaattaatt
     4021 gatcttttaa attaaatatt tacattgaag atgatttatg ttaaaataag attgaagaaa
     4081 ggggtgttaa attatgatac aattgtatat ttctcctagt tgttcatctt gccgtaaagt
     4141 aaaaaagtat tttgacaacc atggaattcc ttatatggaa aaaaacattt tgaacgctcc
     4201 gatatctaaa gaagaaatca tgagaatgct aacaaaatct gaaaatggtt ttgaagatat
     4261 tatctcaacg cgttcaaacg tatttaaaga aaaaggtaat aaaattgagc aaatgaaaac
     4321 tagtgaatta gttgacttca ttatcgataa tcctagtgtc ttaaaacgcc ctattatcgt
     4381 agctgattat gaattacaag tagggtataa tgaagaagac attacgcttt tcttacctca
     4441 agataaaaga gaagctattt gtagtgtttg tttgaagaac aatccagagg attgtgatta
     4501 tattaaacat ttacgttttt ctgataaata aaaaaagcac attttttaat gtgcttttta
     4561 attaaatctt tttaatgctt cataagtacg aataattttc ttttcgcact ttttataaac
     4621 gatattaaaa tctctaatta actctacgaa ttctttacgg ccttggtagt agtttgtagt
     4681 tgggttatag taaagttcat agtctgttat cccgcaatat gtagatttat caagataaac
     4741 aactccgttt ttacattgca taaaaagacg gattgttgaa agactcataa agtttacaag
     4801 tttttgatct ttaattattt ttttaacttc aggttttaca tcgcctttag gaatatcgcc
     4861 agtttcaatc atattctcta attgctcttt tgtaattata tcactaaaag ttactgtttc
     4921 atagcttttc ttgatcttga aaactaattc taaagtttct cgttcacgga ctcttaacga
     4981 acagtcttca gcttttaatg aaaaacgtgt agtatcaaaa taatgatttg tttgaaagtc
     5041 aggattactg aaattagaca ttattcgctc atattcttcc tgagttaaaa gtgtacgcaa
     5101 atctctattt gtagtagcca tatatatcct ccttatagtg atatattgta ttatactaaa
     5161 aattttcaaa aatgcaagta atttataaaa aaatatataa aatttaaaaa ggagtgattt
     5221 ttttgaattc aaatgattgg caaagttttc ttttacctta tgaattagcg gtagaaggtt
     5281 ttattgtaaa actagaatca attcgtaaac agtacatttt aaaaggttta tacaatccaa
     5341 ttgagattgt aactggtcgt gttaaaacag ttgaggcaat cttacaaaaa gcaacaagac
     5401 ttgagataga gtcttcagat attccagaaa aaattcaaga tattgctgga gtaagaatta
     5461 tttgtaagta tattgatgat gtatacttag ttcaagactt aattacttca agaagagatt
     5521 tgaaaattat tacaattaaa gattatgtaa agaaaccaaa acctagtggc tatcagtctt
     5581 tacatattat tgctcagtat gttacagaaa caattgaagg acctaatact atttatattg
     5641 agtttcaggt aagaactcat gttatgcatt tttgggctag catcgagcac tcattaaact
     5701 ataagtatga ttaccaaatt ccaggtacaa taaaatttag attactaaaa gcaagtcaag
     5761 ctgcgcaaaa attagatgta gaaatgacta agattaaaaa gacaattgat aaattagaag
     5821 aaaaacacga acttaaaaaa gccaagaatc ctttagaaga aaaggaatat aaattaaatt
     5881 cagtgaggaa ggaataaaga tgaaatatag ttatgtaggt aaaatgccag ataaaataaa
     5941 aacctattta aaagaagata tctattcaaa agaaaatcca gaatatgttt tttcttttgg
     6001 tggtgatggt acaatgctta aagcagtaga agagtatctt catctagcag
//
LOCUS       DAOPNX010000122         5660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_15639_length_5660_cov_6.586084, whole genome shotgun sequence.
ACCESSION   DAOPNX010000122 DAOPNX010000000
VERSION     DAOPNX010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15639_length_5660_cov_6.586084"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1570
                     /locus_tag="PKN53_10190"
     CDS             146..1570
                     /locus_tag="PKN53_10190"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOD89358.1"
                     /translation="MKKYVLLLILVLFSASIFSQEKWTLQKCIQHALDNNIQIKSQEY
                     NSKIHDINLQKAKYGILPGLNVGASENLTFGRNVDPYSYQFSTDNFHSANFQISSSVT
                     LFSGLQQYNSIKKAEIDLEAGNASLEQTKNNVMLAVASAYLNVLYALDLVEIAEQQKE
                     ITELQLERTEKLVASGSLPIQSKYDVEAQLANEELNLVNYRNQYDFAVLNLAQLIEYE
                     DFSNMEIQKPDVDKLIGESVLLSPEQIFDEAVNNLPQVKYAELNYLSSQKALQISKGA
                     FYPRLTMSATYGSGYSSLTKFVEGIKLGNPMLSGFATDNLGNLLDVYQYTFDYSYVNK
                     PFFDQIKDNASTSVGFYLNIPIFNGLQTRSGVNSSKLYLEQAKLQVDQAKKDLFKEIQ
                     QAWSDAVSAMKKFDASEKAFEASNLSFEYTQKKYEQGLVNITDYNIAKNNVSRTQIEM
                     LKSKYDFIFKQKILDFYRGLSFNF"
     gene            1580..2944
                     /locus_tag="PKN53_10195"
     CDS             1580..2944
                     /locus_tag="PKN53_10195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD89359.1"
                     /translation="MRKNKSKSKKKRTRIIIFGIIGLIIVLIIAKKAGLFGSSDIYEV
                     STEIVEKRTITEIVNASGKIQPAMEIKLSPDVSGEIVELAVREGCEVKKGDFILKIKP
                     DLYESVLERTIAAGNSANANLENSKARLEQVKAQFIQTKLSHQRNETLYEQGVISQSE
                     FENSLAAYQMGLADVSAAEASVNAAKYSIMSSNASIKEATENLRKTSVFAPMDGTVSL
                     LNVEVGERVVGTEMMAGTEMMRIAKLEDMEVIVDVNENDIVKIKLNDSADIEVDAYLK
                     RKFAGVVTEIANSASSSGVSINQVTNFKVKVKILNESYADLVTENNKYPFRPGMSASV
                     NIHTNTVNDVISLPLAAVTTRVDSVNMKTPTNVEGNKDNQENKTIDKSELQEVVFVYK
                     DGKVEQRNVKTGIQDSYYIQIIEGVELKEEVVVAPYNLISKILKNGTSVKKLSKEELY
                     KPKK"
     gene            2993..3718
                     /gene="lptB"
                     /locus_tag="PKN53_10200"
     CDS             2993..3718
                     /gene="lptB"
                     /locus_tag="PKN53_10200"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOD89360.1"
                     /translation="MKLHTSELVKQYGKRTVVKGVSVEVKQGEIVGLLGPNGAGKTTT
                     FYMIVGLIKPKSGKIFLNDIEITNEPIYKRARLGIGYLAQEESVFQKLSVQDNIRAIL
                     EMTKLSRKEQQYKTNQLLEEFGLTHIKKSKGFQLSGGERRRTEIARALAIDPKFLLLD
                     EPFAGVDPIAVEDIQTIVRHLKDRNIGVLITDHNVHETLSITDRAYLLFEGDILKSGT
                     AQELADDEQVRRLYLGQNFELRQ"
     gene            3814..5478
                     /locus_tag="PKN53_10205"
     CDS             3814..5478
                     /locus_tag="PKN53_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOD89361.1"
                     /translation="MKNNIFRPKFFSILKQGYDKKQLSKDVFAGIIVGVVALPLAIAF
                     AVASGVSPEKGIVTAIIAGFIISFLGGSRVQIGGPTGAFIVIVFGIVTQYGIDGLLIS
                     TILAGIMLVIFGVLRLGALLKYIPHPLIVGFTSGIALIIFSTQMKDAFGLEIANVPAD
                     FLGKWEAYFTNYSTVNFVALAITVATILISTLFRRITNKIPGSFVAIILMTLVVYFFE
                     LPVTTIETLFGDIKGSFAIDIPDINWGNISYYFAPAFTIALLGAIETLLTAVVADGMI
                     GSNHRSNTELIAQGIANIVTPIFGGIPATGAIARTATNVKNGGRTPVAGMVHAVVLLL
                     ILLFFGKYAKLIPMSCLAGILIVVAYNMSEWRSFVSILRGSAFDIIILLTTFTLTVLV
                     DLTVAIQIGVVLSAFLFMKRMTDTGQAAILKENESEMIENYGHVPKGIEIYEISGPFF
                     FGAAKHYGEVLKGHASSTKVLIIRMRHVPFIDATGVHNFREAIKVLHSKNKRIILSGV
                     QPNVRKELEKSEIAQRIGEENIFDNFDAALAKATEVVAEKQEAVTK"
BASE COUNT         2098 a          759 c         1030 g         1773 t
ORIGIN      
        1 agagaaggtg gcttgcatga cactgcagga aaaatggaaa atttattaaa tgcttataaa
       61 taaaagatta aaaaatattt tttaatcttt tattaataat atggtattcg ttttttttat
      121 accttagcaa aatatttctt agctaatgaa aaaatatgtt ttgcttttaa tcttagtgct
      181 tttttctgcg agtatatttt ctcaggaaaa atggacttta caaaagtgta ttcaacatgc
      241 tttagataat aatattcaaa ttaaatctca agaatataat tccaaaatac acgacataaa
      301 tttacaaaaa gcaaaatatg gaattttacc tggattaaat gttggagcta gtgaaaattt
      361 aacttttgga cgtaatgttg atccatattc ctatcaattt tcaacagata attttcattc
      421 tgcaaatttt caaatatctt cttctgtaac tcttttcagt ggtttgcagc aatataattc
      481 aattaaaaaa gcagaaattg atttagaagc aggaaatgca agtttagagc aaacaaaaaa
      541 caatgtgatg ttggcagttg cttcggctta tttgaatgta ttgtatgctc ttgatttagt
      601 tgagattgca gaacagcaaa aagaaattac agaattacaa ttagaacgta ccgaaaaact
      661 tgtggcttct ggaagtttgc caatacaatc aaaatatgat gtcgaggctc agttagcaaa
      721 cgaagaattg aatttggtaa attacagaaa tcaatatgat tttgctgtgc ttaatttggc
      781 tcagttgata gaatatgaag atttttcaaa tatggaaatt caaaaaccag atgttgataa
      841 attgatagga gagtctgttt tgcttagtcc agaacaaatt tttgatgaag ctgtaaataa
      901 tttgccacaa gttaaatatg cagaactaaa ttatttaagt tctcaaaaag ctttacaaat
      961 ttcaaaagga gctttttatc ctcgtttaac aatgagtgct acttatggtt caggatattc
     1021 tagcttgaca aaatttgttg aaggaataaa acttggaaat ccaatgctct cgggttttgc
     1081 aacagataat cttggcaatt tattagatgt ttatcaatat acttttgatt attcttatgt
     1141 aaataaacct tttttcgacc aaattaaaga taatgcttca acttctgtag gtttttattt
     1201 gaatattcca atttttaacg gtttgcaaac gaggtctgga gtaaattctt caaaacttta
     1261 tttagaacaa gcaaaactac aagttgacca agcaaaaaaa gatttattta aagaaattca
     1321 acaagcttgg tctgatgctg tttcggcaat gaaaaaattt gatgctagcg aaaaagcttt
     1381 cgaagcaagt aatttgagct ttgaatatac tcaaaagaag tatgagcaag gattagtaaa
     1441 tattacagac tataatattg ctaaaaacaa tgttagtcgc acacagatag aaatgttaaa
     1501 atcaaaatat gattttattt ttaaacaaaa aatattagat ttttatagag gactaagttt
     1561 taatttttaa aaatcgaaaa tgagaaagaa caaatcaaaa tcaaagaaaa aaaggacaag
     1621 aattataatt ttcggaatta taggactaat aattgttctg ataattgcaa aaaaagcagg
     1681 tctttttggt tcatctgaca tatatgaggt aagtacagaa attgttgaaa aacgtacaat
     1741 cacagaaatt gtaaatgcta gcggaaaaat acagccagca atggaaataa aattaagccc
     1801 tgacgtttcg ggagaaattg ttgaacttgc tgttagagaa ggctgtgaag ttaaaaaagg
     1861 agattttatt ctaaaaataa aacccgacct atacgaatct gtacttgaaa gaactattgc
     1921 tgcaggtaat tcagcaaatg caaatttaga aaattcaaaa gcaagattag aacaagtaaa
     1981 agctcaattt atacaaacaa aattatcaca tcaacgtaac gaaactttgt atgaacaagg
     2041 agtaatttca caatcagagt ttgaaaattc tttggcagct tatcaaatgg gattagcaga
     2101 tgtaagtgca gctgaagcta gcgtaaatgc agcaaagtat tcaataatga gttcaaatgc
     2161 atcaattaaa gaagcaacag aaaatttaag aaaaacttca gtatttgcac caatggacgg
     2221 cacagtttct ttgctaaatg ttgaagtagg cgagagggtt gttggtacag aaatgatggc
     2281 aggaacagaa atgatgagaa ttgccaaact tgaagatatg gaagttattg ttgatgtaaa
     2341 tgaaaatgat attgtaaaaa ttaaacttaa cgactcagca gatatagaag ttgacgcata
     2401 tcttaaaaga aaatttgcag gggtagttac agaaattgca aactcggcaa gttcttcagg
     2461 tgtgtcaatt aatcaagtta caaactttaa agtaaaagta aaaattttaa acgaatctta
     2521 cgcagattta gttactgaaa ataataaata tccatttaga ccaggaatgt cggctagcgt
     2581 taatattcat acaaataccg tgaacgatgt gatttcacta ccattagcag cagttacaac
     2641 aagggtagat tctgtaaata tgaaaactcc taccaatgta gaaggaaaca aagacaatca
     2701 agaaaataaa actatagata aatctgagtt gcaagaagtg gtttttgtgt ataaagacgg
     2761 aaaagtagaa caaagaaatg tgaaaacagg aattcaagat tcatattata tccagattat
     2821 tgaaggagtt gagttaaaag aagaagttgt tgttgctcct tataatctta tatctaaaat
     2881 cttgaaaaat ggaacttcgg taaagaaact tagtaaagaa gaactttaca aaccaaagaa
     2941 ataatatatt tgaaaacaga tatttttata tatttttgta aaaaatatta aaatgaaact
     3001 tcatacttct gaattagtaa agcaatatgg aaaacgcact gttgtaaaag gtgtgagtgt
     3061 agaagtaaaa caaggcgaaa tagttggatt gttaggacct aacggagccg gaaaaacaac
     3121 tactttctat atgattgtag gattgataaa acctaaatca ggaaaaattt ttcttaacga
     3181 tatagaaatt actaatgaac caatttacaa gcgagctaga ctaggtatag gttatcttgc
     3241 acaagaagaa tctgttttcc aaaagctaag cgttcaggat aatataagag caattcttga
     3301 aatgacaaaa ttgtcgcgta aagaacagca atacaaaacc aatcaactgc ttgaggaatt
     3361 tggtcttaca catataaaga aaagtaaagg atttcaactt tcgggaggcg aacgccgaag
     3421 aacagaaatt gcaagagctt tagctataga cccaaaattc cttttacttg atgaaccctt
     3481 tgctggagtt gacccaattg cagtagaaga tattcaaacc attgtgagac acttgaaaga
     3541 tagaaatata ggtgttctaa ttaccgacca taatgtacac gaaactttaa gtattacaga
     3601 tagagcttat ttacttttcg agggagatat tttaaaaagt ggaactgcac aagagttggc
     3661 agacgatgaa caagtaagaa gactttattt gggacaaaac tttgaattac gccagtaatt
     3721 atgattatga aacatgttaa ataattagtt tctgacactt tttatgacta attttgcaca
     3781 ctaaaaaatt aaaatcttag aattacttag aatatgaaaa ataatatttt ccgtcctaaa
     3841 tttttctcga tattaaaaca aggatacgat aaaaagcaat tatcaaaaga tgtatttgca
     3901 ggaattattg ttggtgtagt ggctttgcct ttggcaattg cttttgctgt ggcttcgggt
     3961 gtgtcgcccg aaaaaggaat tgttactgct attattgcag ggtttataat ttctttctta
     4021 ggtggttcta gagtgcaaat tggcggtcct acaggtgctt tcatcgttat agtatttgga
     4081 attgttacac aatatggaat agatggattg ctgatttcaa caatattagc tgggattatg
     4141 cttgtcattt ttggagtgct gaggttagga gcattactaa aatatattcc acatccattg
     4201 attgtgggat ttacttccgg aattgcactt ataatttttt ccacccaaat gaaagacgct
     4261 tttggacttg aaatagcaaa tgtgccagca gattttttag gaaaatggga agcatatttt
     4321 acaaattata gtacagtgaa ttttgtagca cttgcaatta ctgtagcaac tatcttgatt
     4381 agtacattgt tccgtagaat tactaataaa ataccaggct cttttgttgc aataatacta
     4441 atgactttag ttgtatattt ctttgaattg cctgtaacta caattgaaac attgtttggc
     4501 gatattaaag gctcttttgc aattgatata ccggatatta attggggaaa tattagttat
     4561 tattttgcac cagcatttac cattgcctta cttggagcta tagaaacttt gttgacggca
     4621 gtagtggcag atggtatgat aggctctaat catagaagta acacagaact tattgctcaa
     4681 ggaattgcaa atattgtaac tccaattttt ggaggaatac cagcaacagg agctattgca
     4741 agaacagcaa caaatgttaa aaatggtgga agaactcctg tagcaggaat ggttcatgct
     4801 gttgttttat tgcttatttt attatttttc ggtaaatatg ctaaactgat accaatgtcc
     4861 tgtcttgcgg ggatattgat tgtagtagct tataatatga gtgaatggag atctttcgtt
     4921 tctatactta gaggttcagc ttttgatata attattctat taacaacatt tactttaaca
     4981 gttttagtgg atttaacagt agctattcaa attggtgttg tactttcagc attcttattt
     5041 atgaaacgta tgactgacac tggacaagca gcaatattga aagaaaacga aagcgaaatg
     5101 attgaaaact acggacatgt tccaaaagga attgaaatct atgaaattag tggacctttc
     5161 ttttttggag cagcaaaaca ttatggcgaa gttttaaaag gacatgcatc ttcaacaaag
     5221 gttttgatta ttcgaatgcg acatgtgcca tttattgacg ctacaggagt gcataatttt
     5281 agagaagcaa taaaagtttt acatagtaaa aataagagaa ttatattatc tggtgtgcaa
     5341 ccaaatgttc gtaaagaatt agaaaaatca gaaatagccc aaagaatagg ggaagaaaat
     5401 atttttgata acttcgatgc tgcactagct aaagcaacag aagtagttgc agaaaaacaa
     5461 gaagccgtga caaaataaat cagttaaaag tttataaagt tcataaagtt cataaagacg
     5521 ttatattttt gttactacaa atccgagcga gcgtcatggg taattggatt gcgagcagag
     5581 tgagcttgcg aactctgcga agcaatagac gcgtcagcct cttttaacac ttcaactacg
     5641 ttcagtgcat cgcttttaac
//
LOCUS       DAOPNX010000123         5513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_16175_length_5513_cov_7.120740, whole genome shotgun sequence.
ACCESSION   DAOPNX010000123 DAOPNX010000000
VERSION     DAOPNX010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16175_length_5513_cov_7.120740"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(285..2432)
                     /locus_tag="PKN53_10210"
     CDS             complement(285..2432)
                     /locus_tag="PKN53_10210"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOD89362.1"
                     /translation="MKKSIIFIILAVFFASEFAFSQTKNLTIDEAVVGVYRELYPEYI
                     RGLNIRPASKEFSKIVEKELILTSFDLKNESVLINLDNLNNALNKIEKDSLKSFPQYK
                     WLSKNTLRFFVRNNIFDYDVSKKQITFYFALSDDFENIDFCEENKKAAFTQDNNLSII
                     DSEGNILKISNEENKDIVFGQTVHRNEFGIEKGTFWSPKGNFLAFYRNDQTMVTDYPR
                     VNTNARIAEATPFKYCMAGMKSEEVTLGIYNVKTQKINYIETGEPKEQFLTNIAWSPD
                     EKHIYIAVLNRGQNHMKLNKYDIETGKFVKTLFEEKHEKYVEPLHPMVFLPNDPSKFI
                     WQTRKDGFSHLYLYNDNGQEISQITKGNFEVQQVYGFTPNGKDILINANKETPIDFDI
                     YRVNIASGAMIRISHNPGSHRAIIAEDYSYLIDNYSSTTAPNEYVIYDLKGKKINTIL
                     SSKNPLEDYNIGEMKISTLKANDGKTDLYYRIILPPDFDASKKYPAIIYVYGGPHAQL
                     VENRWLGGAAGWDYYMAQEGYIMFTLDNRGSANRGLEFENIIFRQLGSVETQDQITGY
                     EYLKSLGYVDTERIGVHGWSYGGFMTTTLMTEHADIFKVGVAGGPVINWELYEIMYGE
                     RYMDTPEENPEGYAQSNLLNKVDKLTGRLMLIHGAVDPVVVWQHSLNFLNKCIEKKVL
                     VDYFVYPSHEHNVRGYDRIHLMRTVTRYFKDNL"
     gene            2482..3294
                     /locus_tag="PKN53_10215"
     CDS             2482..3294
                     /locus_tag="PKN53_10215"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOD89363.1"
                     /translation="MFTKNNILLIVLGFLTIFYNCSPKADEHKTKAKVSESEIEVIDS
                     TELIIEEVEIINETENYISELGYIKITDSLPEILVDLKYSSDDNFLGFNFYGELQNAF
                     VRYECYLKLEKAYKILQNQKPGYTFIIYDALRSVESQQLMWDSVKVEPAKKHWYVANP
                     QKGSIHNFGMALDISIVNEFGEVLDMGTDFDYFGEKAYPSNTEKFYQNGELSEEQYKN
                     RNLLMSVMAEAGFYISKTEWWHYNASSLDYAKSKYEIFSYPININTNDCKEL"
     gene            3275..4297
                     /gene="aroB"
                     /locus_tag="PKN53_10220"
     CDS             3275..4297
                     /gene="aroB"
                     /locus_tag="PKN53_10220"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOD89364.1"
                     /translation="MIVKNYKVKTSSSTNIYVNADYNYYKELAQERELVVITDDVLYA
                     LYPDAFENQKCIVIKSGEENKTLKTVEKIIEQLVEFEADRRTLIIGFGGGMVCDITGF
                     VAGIFMRGASFGFVATTLLAQVDASIGGKNGVNFNNYKNYIGSIIQPEFVICDARVLK
                     TLPEDDYKSGLGEVIKYALISDMDLVEYLNENVENIFNQVPEVLSFIIKKCIGIKAKI
                     VEDDTNDFGLRHILNFGHTIGHSIEIIENIPHGIAVAKGIVEEIKISLKLEYADKSLI
                     EIAENLISKFISDYKCEIRQEHIDLLVKDKKKTGDMINFVFLTNIGEPLIVKTEFKDI
                     IKALKS"
     gene            4306..>5513
                     /locus_tag="PKN53_10225"
     CDS             4306..>5513
                     /locus_tag="PKN53_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89365.1"
                     /translation="MEIFIKPKSFNREITASASKSYEQRFLALSLLTGEKTVLKSFGN
                     SADVIAARNIIKTLGCDFFIKNNKLIIKKRKQFSDDYIPTLDCGESGLCARLFVPVAA
                     VLYKNFIVKASGSLLNRNIYEDYKSLSDFGFNFSSENNNFPFNFYDCELKAGNYKIDG
                     KNSSQLVSGLLMALSCLPEPSVLKVENAVSIKYMQMTVDALQRFGFQLIDEYGETCNF
                     KIYPKRLALDNYKFKIEGDWSGISNVLVAGALIQDIKVKGLNQYSLQADKAILEVFDL
                     AGVRYKWKNSVLKIFKSRVNSFEFDATNCPDLIPAIVILAVFAKGKSLIHGISRLKNK
                     ESSRAEVLQKELAKAGITLQIDGDAMIIQGEQKPNFAVLDSNGDHRMAMAFSILGLNS
                     ENGIKITNADV"
BASE COUNT         1869 a          758 c          934 g         1952 t
ORIGIN      
        1 gttaaaagtt tataaagttc ctaaagttcg taaagtcaga tttataacaa gatgtgtcaa
       61 ctttttttag tacgttacaa aaactttcaa ggtgtttcag aagtgttaaa atgcaaggca
      121 tctgagattg aggacgaagg agcgtaccag aagcggtgca ctgagcaaag ccgaagtgta
      181 cgtgactgag tccgaatatc gaaagcaacg tggttgctga acgaagttga agcaaagcag
      241 tttgatactt atgaaacacc gccttttaac ttttaactga ttttttataa attatcctta
      301 aaatatcttg taacagttct catcaaatgt attctatcat atcctcttac attgtgttcg
      361 tggcttggat atacaaaata gtcaactaaa acttttttct caatacattt attaaggaag
      421 tttaaggaat gttgccaaac aacaactggg tcaacagcac catgaattag cattagtctg
      481 ccagtaagtt tatcaacttt gtttaaaaga tttgattgag catagccttc aggattttct
      541 tcgggagtgt ccatatatct ttctccatac ataatttcgt ataattccca attaattact
      601 ggtccaccgg caacaccaac tttaaaaata tctgcatgtt cagtcattaa agtagttgtc
      661 ataaagccac cgtaactcca gccatgaacg ccaattcttt ctgtgtcaac atagccaagg
      721 ctttttaaat attcgtatcc tgtaatttgg tcttgagttt ccacgcttcc taattggcgg
      781 aaaataatat tttcaaactc taaaccacga tttgccgagc ctctattgtc gagagtaaac
      841 attatatatc cttcttgagc catatagtag tcccagccag ccgctccgcc aagccatctg
      901 ttttcaacaa gttgagcatg aggtccgccg taaacataaa ttattgcagg atatttttta
      961 cttgcatcaa agtcaggtgg taaaattatt ctgtaatata aatcagtttt tccatcattt
     1021 gctttaagtg tagaaatttt catttcgcca atattgtaat cttctaatgg atttttggaa
     1081 cttaatattg tatttatttt tttgcctttt aaatcataaa ttacatactc gtttggagca
     1141 gttgtacttg agtaattatc aatcaaatag gaataatctt cagcaataat tgctctatgc
     1201 gaaccaggat tgtgactaat tcttatcatt gcaccgctgg caatatttac tctataaata
     1261 tcaaaatcta taggagtctc tttatttgca tttattaaga tgtcttttcc gtttggtgta
     1321 aaaccataaa cttgctgaac ttcaaaattt ccttttgtaa tttgtgaaat ttcttgtcca
     1381 ttgtcattat ataaatatag gtgagaaaat ccatctttgc gagtttgcca aataaatttg
     1441 cttgggtcat taggtaagaa aaccattggg tgcaaaggct caacatattt ttcgtgtttt
     1501 tcttcgaaaa gagtttttac aaatttccca gtttcaatat cgtatttgtt gagtttcatg
     1561 tgattttgtc ctctgtttaa aacagcaatg taaatatgtt tttcgtctgg actccaagca
     1621 atatttgtta aaaactgctc ttttggttcg ccagtttcta tataatttat tttttgtgtt
     1681 tttacattgt aaattccaag tgtaacttct tcgcttttca ttccagccat gcaatattta
     1741 aaaggagtgg cttcggcaat tcttgcattt gtatttacac gtggataatc agttaccata
     1801 gtttggtcgt tgcgataaaa agctaagaaa tttccttttg gcgaccaaaa agttcctttt
     1861 tcaattccaa attcgttgcg atgaacagtt tgtccaaaaa ctatatcttt gttttcttca
     1921 ttgctaattt taagaatatt tccttctgaa tcaattatcg aaagattatt atcttgagtg
     1981 aaagctgctt ttttattttc ctcacaaaaa tcaatgtttt caaaatcatc gctgagagca
     2041 aaataaaaag taatttgctt tttagaaaca tcataatcaa aaatattatt tcttacgaaa
     2101 aatcttaaag tgtttttact cagccactta tattgaggaa atgattttaa actatctttt
     2161 tcaattttgt ttaaagcatt atttaagtta tctaaattta ttaaaacact ttcgtttttt
     2221 aaatcaaaac ttgttaaaat aagttctttt tctacgattt tactaaattc tttacttgca
     2281 ggtctgatgt ttaaacctct aatatattcg ggatataatt ctctgtaaac accaacaact
     2341 gcttcgtcta tagttaaatt ttttgtttga gaaaaagcaa attcactcgc aaaaaaaact
     2401 gcaagaataa taaagatgat agattttttc atttctttaa attttttatt aattttacat
     2461 ttacaaagat ataattttta gatgtttaca aaaaataata ttttactgat tgttttaggc
     2521 tttttaacaa ttttctataa ttgttctcct aaagcagatg agcacaaaac caaagccaaa
     2581 gtttcagaaa gtgaaattga agtaattgat tccacagaat tgattattga agaagttgaa
     2641 attataaatg aaaccgaaaa ttatatttca gaacttggat atattaaaat tacagattct
     2701 ttgcccgaaa ttcttgttga cttaaaatat tcaagcgatg ataattttct tggctttaat
     2761 ttttatggcg agttgcaaaa tgcttttgtt cgatacgaat gctatttaaa acttgaaaaa
     2821 gcttataaaa ttttacaaaa ccaaaaaccg ggctatacat ttataattta tgatgctttg
     2881 cgtagtgttg agtctcagca attgatgtgg gattctgtga aagttgaacc tgccaaaaag
     2941 cattggtatg ttgcaaatcc acaaaaaggc tcaattcata attttggaat ggctttagac
     3001 attagcattg taaacgagtt tggcgaagtt ttagatatgg gaacagattt cgattatttt
     3061 ggcgaaaaag catatccaag caataccgaa aaattttatc aaaatggtga gcttagcgaa
     3121 gaacaataca aaaatcgaaa tttgttaatg tctgtaatgg ctgaagcagg tttttatatt
     3181 tccaaaaccg agtggtggca ttacaacgct tcttctttag attacgcaaa atcaaaatat
     3241 gaaatttttt cttacccaat aaatataaat accaatgatt gtaaagaatt ataaagttaa
     3301 aacttcaagt tccactaata tatacgtaaa tgctgattac aattattaca aagaattagc
     3361 tcaagaacgt gaattggtcg taattacaga cgatgttttg tatgctcttt atcctgatgc
     3421 atttgaaaat caaaaatgta ttgtgataaa atctggagaa gaaaataaaa ctttgaaaac
     3481 tgtagaaaaa attattgagc aacttgttga gtttgaggca gataggagaa ctttaattat
     3541 tggttttggt ggtggaatgg tttgtgatat tactggattt gttgcaggaa tatttatgcg
     3601 aggagcaagt tttggctttg ttgcaacaac tttgttagct caagttgatg catctatagg
     3661 tgggaaaaat ggagtgaatt ttaataatta taaaaattat attggaagca ttattcaacc
     3721 agaatttgtg atttgtgatg caagagtttt aaaaactttg cccgaagacg attataaaag
     3781 tggcttggga gaagtgataa aatatgcctt gataagcgac atggatttgg tagaatattt
     3841 aaacgaaaat gttgaaaata tttttaatca agtgcctgaa gttctgagtt ttataattaa
     3901 aaaatgtata ggaattaaag ctaaaattgt tgaagatgat actaatgatt ttggattaag
     3961 gcatatttta aattttggac atacaatagg acatagcata gaaattattg aaaatattcc
     4021 tcatggaatt gctgtggcaa aaggaattgt tgaagaaata aaaatttcac taaaacttga
     4081 atatgccgac aaatctttaa ttgaaatagc agaaaatttg atttcaaaat ttatttcaga
     4141 ctataaatgt gaaattaggc aagaacatat tgatttgctt gtgaaagaca agaaaaaaac
     4201 gggagatatg ataaattttg tgtttttaac taatattggc gagcctttga ttgtaaaaac
     4261 tgaatttaag gatattatta aagccttaaa atcttaattt tgtgtatgga aatttttata
     4321 aagcctaaat cttttaatag agaaatcacc gcttcggcat caaaatctta tgagcaaaga
     4381 tttttagcat tatccttgct tacaggcgaa aaaactgttt taaaaagctt tggaaatagt
     4441 gctgatgtaa ttgctgcaag aaatattata aaaactttgg gctgtgattt ttttataaaa
     4501 aataataaat taattattaa aaaaagaaaa cagttttcag atgattatat ccctacttta
     4561 gattgtggtg aatcgggttt gtgtgcaagg ttgtttgtgc ctgttgcagc ggttttatac
     4621 aaaaatttta ttgtaaaagc atcgggaagt ttgctgaata gaaatattta tgaagattac
     4681 aaaagtttaa gcgattttgg ctttaatttt agttcagaaa acaataattt tccatttaat
     4741 ttttatgatt gtgagctaaa agctgggaat tataaaattg atggtaaaaa ttcctctcaa
     4801 ttagtttcgg gtttacttat ggctttgagt tgtttgccag agccttcggt tttgaaagtt
     4861 gaaaatgctg ttagtataaa atatatgcaa atgacagttg atgctttgca aaggtttggt
     4921 tttcagctta ttgacgaata tggcgagact tgtaatttta aaatttatcc aaaacgatta
     4981 gctttagaca actataaatt taaaattgaa ggcgattggt cgggaatttc aaatgttttg
     5041 gttgcaggag ctttaataca agatattaaa gtaaaaggtt taaatcaata ttctttgcag
     5101 gctgataagg caattttaga agttttcgac ttagcaggag tgaggtataa atggaaaaat
     5161 tctgtattaa aaatatttaa gtcgagagta aattcttttg agtttgatgc aacaaattgt
     5221 ccagatttaa ttcctgccat tgtgatttta gctgtttttg caaaaggaaa gtcattaatt
     5281 catggaatat ctcgattaaa aaataaagaa agctctcgtg cagaggtttt acaaaaagaa
     5341 ttggctaaag caggtataac tttacaaatt gatggcgatg caatgataat tcagggagag
     5401 cagaaaccaa attttgcagt cctagactca aacggcgacc acagaatggc tatggctttt
     5461 agtattttag gtttaaattc cgaaaatggc attaaaatta caaatgctga tgt
//
LOCUS       DAOPNX010000124         5505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_16208_length_5505_cov_6.907156, whole genome shotgun sequence.
ACCESSION   DAOPNX010000124 DAOPNX010000000
VERSION     DAOPNX010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16208_length_5505_cov_6.907156"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PKN53_10230"
     CDS             <1..551
                     /locus_tag="PKN53_10230"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89366.1"
                     /translation="VSTEPTIRKALAIGADDAFRIDCEPSDSYTIAAQIAEVAKNENY
                     DIIFAGIESSDFNAGAVGSMIAEFMDIASVSSVSSFEFNNGDITLKREIDGGKETVKI
                     QTPFVAIVQKGICINPRIPAMRGIMMAKTKPIKVSSPVSTEALSEALSFEVPPPKADC
                     KMIDAENTDELIELLHSEAKVL"
     gene            584..1552
                     /locus_tag="PKN53_10235"
     CDS             584..1552
                     /locus_tag="PKN53_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOD89367.1"
                     /translation="MSIIVFSENRSEKIKKSSLEAVAYATNISKNTQQAVVSVSIGNV
                     PNEELEILAKYGASKIISINAPEWQTHDSQAYAKLLNEITEKENASTIIFTNSNIGKA
                     VAPRLSVRLKAALVSGIVSVPNSYLPLTLKRKSYNGGAFQNVQVKSPKAVLTLNPNSC
                     EVVENPQAVNIENYTPNTPTNFQTKIEATDVTSGKIVLTEASIVVSGGRGLKSGDNWK
                     PLEELADALGAGLACSRPVSDEGWRSHEEHVGQTGKIIAPDLYLAVGISGAIQHIGGV
                     SASKVIVAIDRDKDAPIFKYADYGIVGDAFEVLPRLIESVKKFKNI"
     gene            1618..2901
                     /locus_tag="PKN53_10240"
     CDS             1618..2901
                     /locus_tag="PKN53_10240"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOD89368.1"
                     /translation="MSQIIFSVLLAITLGVFAYSIFKIYQLFKLTKPAYPVRDFGKRI
                     KRTIINGFLQQRIFRNPIAGFMHAMVFWGFCIIIFGSIEMVIDGVAGTERILSVGGIV
                     YDIIMAIGDIFAYIIALFVVIFIIRRAVLKIKRLNGKELKHKNHVDAYIALSLILILM
                     ITLAGLNIFYLAGNSDFVGRFPISELIAPMFAGMQPDTINCLHDIFWWSHILLIFLFA
                     NILPYSKHFHIFMSLPNVFFSRLTPLGKMANMDSITKEVKSMLDPETAFAASTDEGSV
                     PDRFGILDVEDITWKNYLDSLTCTQCGRCTSVCPANITGKELSPRKVIMDIRLRMKMK
                     GPHLVKDSSYSDGKTLIRDFISEEEIWACTTCNACAKECPLNIDHPTFILDLRRYLVM
                     EEAAAPAALNAIFSNIENNGAPWQYSNQDRLNWTE"
     gene            2922..3713
                     /locus_tag="PKN53_10245"
     CDS             2922..3713
                     /locus_tag="PKN53_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOD89369.1"
                     /translation="MNSTKIQVPVMAELFAQGKKPEYLFWVGCAGAFDARYMKVAKAF
                     AKILNHIGTDYAVLGTEESCTGDPARRAGNEMLFQMQALSNIEIFKMYEIKKIITICP
                     HCYNTLKNEYPDLGCECEVVNYTEFLENMIKQGKLKIDKTKLANKIVTFHDPCYLGRA
                     NNIYESPRFLLQSLGINLVEMERNRSFALCCGAGGAQMFKDAEKGDKEIFIERTEDAL
                     KTQAEIIASSCPFCMTMMTDGLKYKNKEEEIQNLDIIEMIAEAMI"
     gene            3789..>5505
                     /locus_tag="PKN53_10250"
     CDS             3789..>5505
                     /locus_tag="PKN53_10250"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOD89370.1"
                     /translation="MIKIILYVVASLLALLLFLLAFNSCRSKSKNIETQDYTQYVNPL
                     IGTGEMGHVFPGATAPFGMVQLSPQTHFEPMHDSLGNYNKDVYKYCSGYQYSDTTIIG
                     FAHTNFSGTGHSDLGDVLIMPTTKKISSSDFQSPTDFNTFASKFSHDSEFASPGYYEV
                     FLENDSILAQLTATERVGFHKYTFKDYNSASIVLDMVYNIYHHEDKNVWTFIRVENDT
                     LITGYRQTQGWARTRTVYFSIQTSKPIINYGHKKFDKTQYQGFYRRFDQENNFPEFAG
                     KDIKAFFEFDMQNDPILEIKVALSPVSTDGAMLNALSEAPNWNFDEIKNQTQKKWNAE
                     LSKIDAEFISHEDKLNFYTALYHTYLSPIIYQDVDKKYRGLDQNIHIAQDFDNYTIFS
                     LWDTYRALHPLFNIIQPERNVDMIKSMLVHYQQSVHKMLPIWSHYANENWCMIGYHAV
                     SVIADAIAKDNGNFDKKLALEASVSTANVRYFDGLGYYIDYNFVPDDKSHSSVSKTLE
                     FAYNDWCISEIAKSLNNKAVQEEYLLRSKNFINVFDTVSGFMRPRLSDLTFRENFDPS
                     NTSGQG"
BASE COUNT         1925 a          886 c          938 g         1756 t
ORIGIN      
        1 ctgtgagtac tgaaccaact ataagaaaag cattagctat aggtgctgat gatgctttcc
       61 gtattgactg cgaaccaagc gattcatata caattgcagc tcaaattgca gaagtagcta
      121 aaaatgaaaa ttacgatatt attttcgctg gtatagaatc ttccgatttt aatgctggtg
      181 cagttggcag tatgattgca gaatttatgg acatagcttc ggtttcgtct gtatcttcat
      241 ttgaatttaa taatggtgat ataacattaa aaagagaaat tgacggtggt aaagaaactg
      301 taaaaattca aactcctttt gttgctattg tacaaaaagg tatttgcata aatccacgta
      361 ttccagcaat gagaggaatt atgatggcaa aaacaaaacc tataaaagtt tcaagtccag
      421 tttctacaga agctctatca gaagctttaa gttttgaagt gccaccacca aaagctgatt
      481 gtaaaatgat tgatgctgaa aatacagatg agctgattga acttttacat agcgaagcta
      541 aagtgttata aattagagtt ttattaaatt taaaaattag attatgtcaa ttatagtttt
      601 ctcagaaaat agaagtgaaa aaattaaaaa aagtagctta gaagctgttg cttacgctac
      661 taatatttca aaaaacaccc aacaagctgt tgtttcagtt tctattggta atgtgccaaa
      721 cgaagaactt gaaattttag caaaatacgg tgcttcaaaa ataatttcta tcaatgctcc
      781 agaatggcaa acgcatgata gtcaagcata tgcaaaactt ttaaacgaaa ttactgaaaa
      841 agaaaatgca agcacaatta ttttcacaaa ttcaaacata ggtaaagctg ttgcaccaag
      901 actttcagtt agattaaaag ctgctttggt tagtggtata gttagcgtgc caaattcata
      961 tttaccatta actttaaaac gcaaatcata taatggcgga gcttttcaaa atgtacaagt
     1021 aaaatcacca aaagcagttt taacattaaa tccaaattct tgcgaagttg tagaaaatcc
     1081 tcaagctgta aatattgaaa attatacacc aaatactcct acaaattttc aaactaaaat
     1141 tgaggcaaca gatgttacaa gtggcaaaat tgttcttacc gaagcaagta ttgttgtttc
     1201 tggtggtcgt ggtttaaaat ctggcgacaa ttggaaacca cttgaagaat tagctgacgc
     1261 tttaggtgct ggcttagcat gttctcgccc agtttctgac gaaggctggc gttctcacga
     1321 agaacatgtt ggacaaactg gaaaaattat tgctccagac ctttatcttg cagttggaat
     1381 ttccggtgca attcaacata ttggcggtgt gtctgcttct aaagttattg tagcaataga
     1441 tagagataaa gatgctccta tttttaaata tgccgactac ggaattgtag gagatgcttt
     1501 tgaagtattg ccaagattga ttgagtctgt gaaaaaattt aaaaacatat aaaatcattt
     1561 ttttaattcg ctcaagatta tatttttgaa caagttttat tttaattaaa attatttatg
     1621 tcacaaatta ttttttctgt tctattagca ataactttgg gcgtttttgc ctattctatt
     1681 ttcaaaatat atcagttatt caaattaact aagcctgcct atcctgttcg cgattttggt
     1741 aaaagaatta aaagaacaat cataaacgga tttttacaac aaagaatttt ccgcaaccct
     1801 attgcaggtt ttatgcacgc aatggttttt tggggatttt gcataattat tttcggaagt
     1861 attgaaatgg taattgatgg cgtagctgga accgaaagaa ttttatcagt aggcggaatt
     1921 gtttacgata ttataatggc aattggcgat atttttgcct atataattgc attatttgtt
     1981 gtgattttca tcattagacg tgctgtatta aaaataaaaa gacttaatgg caaagagtta
     2041 aaacacaaaa accatgttga tgcttatata gctctaagcc ttattttaat tttaatgata
     2101 actcttgcag gactaaatat tttttacctc gcaggaaaca gtgattttgt tggcagattt
     2161 cctataagtg aactcattgc tccaatgttt gctggtatgc aaccagatac tattaactgt
     2221 ttacacgata ttttttggtg gtcgcatatt ttattgattt tcctatttgc aaatatactt
     2281 ccttattcaa aacacttcca catatttatg tcattaccta atgttttctt tagtcgtttg
     2341 acaccactag gaaaaatggc aaatatggat tctattacta aagaagtaaa atcaatgtta
     2401 gacccagaaa ctgcttttgc agctagtact gacgaaggaa gtgttcctga ccgttttgga
     2461 attttagatg ttgaagatat tacttggaaa aattatcttg actctttaac ctgtactcaa
     2521 tgtggacgtt gcacatcagt ttgtcctgct aatattacag gaaaagaatt gtcgccacga
     2581 aaagtcatta tggatattcg cttgagaatg aaaatgaaag gaccacattt ggtaaaagac
     2641 tcttcttact cagatggaaa aactttaatt cgtgatttta ttagcgaaga agaaatttgg
     2701 gcatgtacaa cttgcaatgc ttgtgcaaaa gaatgtcctc taaatatcga tcatcctact
     2761 tttatcttgg atttaagaag atatttagta atggaagagg ctgctgcacc tgctgctctt
     2821 aatgcgattt tctcgaatat tgaaaataac ggtgccccat ggcaatactc aaatcaagat
     2881 agattaaact ggacagaata aaaaatttaa aacttagaaa aatgaactca acaaagatac
     2941 aagtaccagt aatggcagaa ttgtttgcac aaggtaaaaa acctgaatat ttattttggg
     3001 ttggttgtgc cggcgctttt gatgctagat atatgaaagt tgcaaaagca tttgctaaaa
     3061 ttttaaacca tataggaacc gactatgctg ttttaggaac agaagaatca tgtactggcg
     3121 accctgctcg ccgtgctgga aacgaaatgc ttttccaaat gcaggcacta agtaatattg
     3181 aaattttcaa aatgtatgaa atcaaaaaaa tcattactat ctgtccacat tgttacaaca
     3241 ctcttaaaaa cgaatatcca gatttaggtt gcgaatgcga agttgtgaat tataccgagt
     3301 ttttagaaaa catgataaaa caaggcaaat taaaaattga caaaacaaaa cttgccaaca
     3361 aaatcgttac tttccacgac ccttgttatt taggacgtgc aaacaacatt tacgaatctc
     3421 cacgtttctt actacaaagt ttaggaatta atttagtaga aatggaaaga aacagaagtt
     3481 ttgctctttg ttgtggtgct ggcggtgcac aaatgtttaa agatgcagaa aaaggcgata
     3541 aagaaatttt tattgaaaga actgaagatg ctcttaaaac ccaagctgaa attattgcaa
     3601 gctcctgccc tttttgcatg acaatgatga ctgatggttt aaaatacaaa aacaaagaag
     3661 aagaaatcca aaatttagac atcattgaaa tgatagctga agcaatgatt taaagaaaaa
     3721 atactatccg aaaatcttta ttatattatc atttttttta cctttgtaac aatacaaaaa
     3781 tatctaaaat gataaaaatt atactatacg tagttgcttc attattagca ttattattgt
     3841 ttttgcttgc atttaattct tgtcgtagca aaagcaaaaa tattgaaact caagattata
     3901 cacaatatgt aaatcctctt ataggcacag gcgaaatggg acatgttttt cctggagcca
     3961 cagctccatt cggcatggta caactaagtc cacaaactca ttttgaacct atgcacgata
     4021 gcttgggaaa ttataacaaa gatgtttata aatattgctc tggatatcag tattccgaca
     4081 ctaccatcat aggttttgct catacaaact ttagtggcac aggtcattcc gatttaggag
     4141 acgtacttat tatgcctact acaaagaaaa ttagctcttc tgactttcaa tctccaaccg
     4201 atttcaatac atttgcttct aaattcagtc atgatagcga gtttgcttcg ccaggatatt
     4261 atgaagtttt tcttgaaaac gattcaattt tagctcaact aactgccacc gaaagagtag
     4321 gatttcataa atatactttt aaagattaca atagtgcaag tattgtgtta gatatggttt
     4381 ataatattta tcatcacgag gataaaaatg tttggacttt cattagagtt gaaaacgaca
     4441 cccttatcac tggctaccga caaacacagg gttgggcaag aactcgcaca gtatatttta
     4501 gcattcaaac atctaagcca attataaatt acggacataa aaaatttgac aaaacccaat
     4561 atcaaggttt ttacagacgt ttcgaccaag aaaacaactt tcctgaattt gctggaaaag
     4621 acataaaggc tttttttgaa tttgatatgc aaaacgaccc tattttggaa attaaagtgg
     4681 ctttatcgcc agttagtaca gacggagcca tgctaaatgc tcttagcgaa gcacctaatt
     4741 ggaatttcga tgaaataaaa aatcaaactc aaaaaaaatg gaatgcagaa ttatccaaaa
     4801 ttgatgcaga atttatttct catgaagaca aattaaattt ttatacagct ttgtaccaca
     4861 catatttgtc gcctattatt taccaagatg tagataaaaa atacagaggc ttagaccaaa
     4921 atattcatat agctcaagac tttgataatt acacaatttt ttctttgtgg gacacttatc
     4981 gagctttgca tcctttattt aatattatac aacccgaaag aaatgtggat atgataaaat
     5041 caatgttggt gcattatcaa caaagtgttc acaaaatgtt acctatttgg agtcattatg
     5101 caaacgaaaa ttggtgtatg attggttacc acgctgtttc ggttatagcc gatgctattg
     5161 caaaagacaa cggcaacttc gacaaaaaat tagcattaga agcttctgtt agtacagcaa
     5221 atgtacgata ctttgacggc ttgggttatt atatcgatta caatttcgtt ccagacgaca
     5281 aaagccattc ttcagtttca aaaactcttg aatttgcata caacgattgg tgtatttccg
     5341 aaatagctaa aagtcttaat aacaaagccg ttcaagaaga atatctacta agaagtaaaa
     5401 atttcataaa tgtatttgac acagtcagcg gttttatgcg acctcgctta tctgacttaa
     5461 ctttcagaga aaattttgac ccctcaaata cttctggaca aggat
//
LOCUS       DAOPNX010000125         5208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_17376_length_5208_cov_5.792742, whole genome shotgun sequence.
ACCESSION   DAOPNX010000125 DAOPNX010000000
VERSION     DAOPNX010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17376_length_5208_cov_5.792742"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..1547
                     /locus_tag="PKN53_10255"
     CDS             225..1547
                     /locus_tag="PKN53_10255"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOD89371.1"
                     /translation="MKKIVFYFAIFVLLNNISFSQEVGFFKSQVVFEPKNSGYFVFRI
                     PSFIITKNNSILAFAEGRKGGGGDWDPSNVVLKTSADGGNTWSDIRVIAEYGKFSCSN
                     IVPIMDYFENKIHLLYVVGYNTVYHTFSTDEGENWEAPINITKNIEDFRSKYNWRVVA
                     TGPGHGTQLTNGRLIVPVWFADASILDTISFIIPHYPSITSVLYSDDFGKSWLMGDIV
                     SPNSDTLVFPSEAVCVELQDGRTMFNMRSESINYRRIVSYSYDGVANWQKPYYSDNFF
                     EPICHASMIRYSIQPYQSKNRILFANPDSRMSPWKLGRATTINAGPKRQRVNLTLRIS
                     YDEGVTFPIYKILDEGRSGYSDLAVDETGIIHCLYEKGSKNNNHFLPENISMLSFDLN
                     WATNGQDHLSPDDMPLNSNMICAAEKYNNKDQVKKKKFFFKRKAQKSC"
     gene            complement(1544..2647)
                     /locus_tag="PKN53_10260"
     CDS             complement(1544..2647)
                     /locus_tag="PKN53_10260"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOD89372.1"
                     /translation="MKDNKHDILNNSDCFSEEILVKFVNDELNDEELAVVENHILNCE
                     ICADFVEGILRLEDPEDYFKVKQKLNIEISKIISTRNIYRKKRKISLQSYYKYAAMIA
                     ILFIASGIFLIISKINSHKTTKQTQIVQETSSPIIFSDVESEDSLVAEQIAQVVSEPK
                     AKDKNQTKSSLVQNEILMVVEDEVTVSLPSDDVIIDEIAINTACEEVAQDKFATLPLE
                     DLDIAESTGALSQYEEVAPKASFNKKKTASKVALGEKGKSDYEFADCEKVENIETEPV
                     FQGDLEEFIKNNLVYPQIYINDSIETIVSVEFTIDSLGNCIMPKILNGEDTYFNNEAI
                     KLINKMPKWEPATLNSQKVNFIYTLDIKFSLKD"
     gene            complement(2637..3218)
                     /locus_tag="PKN53_10265"
     CDS             complement(2637..3218)
                     /locus_tag="PKN53_10265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD89373.1"
                     /translation="MQKLPNNILLLSDLELIEKYKKSKNKEIVGELFKRYSGFCFSIC
                     LKYLKNKELAQEAVLIIFGDLIDKLLIYEVSNFKSWLHSVCRNHCLLYFRNLKQEEKL
                     FANYSSDLKIFMEKDEDFHLSSEKKEIEIEKLKNCISLLKQEQKLCVELFYLQSKSYV
                     EIAQITGFSLKEVKSFLQNGKRNLKICMEKNER"
     gene            complement(3288..4223)
                     /locus_tag="PKN53_10270"
     CDS             complement(3288..4223)
                     /locus_tag="PKN53_10270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD89374.1"
                     /translation="MKKIKILCLSLFITIANICSSQEYVPIVETGNYFSLGSSICGTN
                     IITSTHYEIIGEDTVIGEQTYKKLYESESHVEYHPLINKQLIGFLRETDDRKVYARNL
                     YGHEGLIYDYYVEKGDTLNFRNPFLMADCSLYDLYCGGMSEPCDTFMIVDSIYYAEIN
                     GESRKHYLLNVCYFDFPDLYIEGVGGLNGFHDCGREQIPITGGPNHLFCAYKNDNFYY
                     QNPHYNFCFFTNTEDFNITEIQIYPNPATSILNIVSEENIEYVEILNISGQVVNYKTV
                     SNNIAKVDISALSSGVYFAKIYLSDNILVSKFVVE"
     gene            complement(4246..5181)
                     /locus_tag="PKN53_10275"
     CDS             complement(4246..5181)
                     /locus_tag="PKN53_10275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD89375.1"
                     /translation="MKNLKILCLSLFVTIASICSSQDYVPIVETGNYFSLGSSICGTN
                     IITSTHYEIIGEDTVIGEQTYKKLYESESHVEYHPLINKQLIGFLRETDDRKVYARNL
                     YGHEGLIYDYYVEKGDTLNFRNPFLMADCSLYDLYCGGMSEPCDTFMIVDSIYYAEIN
                     GESRKHYLLNVCYFDFPDLYIEGVGGLNGFHDCGREQIPITGGPNHLFCAYKNDNFYY
                     QNPHYNFCFFTNTEDFNITEIQIYPNPATSILNIVSEENIEYVEILNISGQVVNYKTV
                     SNNIAKVDISALSSGVCFAKIYFLDNVLVRKFVVE"
BASE COUNT         1789 a          836 c          664 g         1919 t
ORIGIN      
        1 ttggaaggtc aaaaacactt ccttttttta tactaactgg tataacttca tgtttggctt
       61 ctaataatac ttcaaccgct ttgtacgaat accgtctagg atttgtactt gctcctaaaa
      121 ctactgttat catattttta tattttttac aaaattaata ttaaaattga ttgaaagtaa
      181 aaaaaacgtt tatatttacc tttttatttt attataaacc tattatgaag aaaattgttt
      241 tttatttcgc aatatttgtt ctgctgaata acataagttt ttcacaagaa gtcggttttt
      301 tcaagtctca agtagttttt gaaccaaaaa attctggata ttttgttttt agaattccat
      361 cttttataat aactaaaaac aacagtattt tagcttttgc cgaaggacgt aaaggtggtg
      421 gtggcgactg ggacccttcc aacgttgttt taaaaacgag tgcagatggc ggaaatactt
      481 ggtcagatat tagagtaatt gctgaatatg gcaaattttc gtgttccaac atagtaccaa
      541 ttatggatta ttttgagaat aaaattcatt tattatatgt agttggttat aatactgtat
      601 atcacacttt tagtactgat gaaggcgaaa actgggaagc acctattaat attactaaaa
      661 acattgaaga ttttcgaagt aaatataatt ggcgagttgt tgctacagga ccaggtcatg
      721 gcacccaact aacaaatggg cgactaattg ttcctgtttg gtttgctgac gcatcgattt
      781 tagacactat ttcctttatt atcccacatt atccgtcaat aacctcggtt ttatatagcg
      841 acgattttgg aaaatcttgg ttaatgggag atatagttag cccaaattca gacactttgg
      901 ttttcccaag tgaagctgtc tgtgttgaac tacaagatgg aagaaccatg tttaacatga
      961 gaagtgaatc cataaattat agaagaatag tttcttacag ctatgatgga gttgcaaact
     1021 ggcaaaagcc ttattattcc gacaactttt ttgaaccgat ttgtcatgct tctatgatta
     1081 gatattcaat acaaccatat caaagtaaaa acagaatttt atttgcaaac cctgatagca
     1141 gaatgtcgcc atggaaacta ggaagagcaa ctacaataaa tgctggacca aaaagacaaa
     1201 gagttaatct tactttaaga attagttatg acgaaggcgt tacattccct atttacaaaa
     1261 tacttgacga aggaagatct gggtattcag atttagcagt tgatgaaaca ggcattattc
     1321 attgccttta tgagaaaggt tctaagaaca acaatcattt tcttcccgaa aacatcagta
     1381 tgttaagctt tgatttgaat tgggcaacaa acggacaaga tcatttatct cctgatgaca
     1441 tgccgctaaa tagcaatatg atttgtgctg ccgaaaaata caataataaa gaccaagtta
     1501 aaaagaaaaa atttttcttt aaaagaaaag ctcagaaaag ttgctagtct tttaagctaa
     1561 attttatatc taaagtataa ataaaattta ctttttggga attgagtgtt gctggctccc
     1621 acttaggcat tttgtttatt aattttattg cttcattatt aaaataggta tcctcgccat
     1681 ttaatatttt tggcattatg caatttccta aactgtctat tgtaaactca acgcttacaa
     1741 tggtttcaat tgaatcattt atataaattt gaggatatac caaattgttc ttaataaatt
     1801 cttccagatc tccttgaaaa actggctctg tttcaatatt ttccaccttt tcacagtctg
     1861 caaattcata atctgattta cctttctctc caagagcgac ttttgatgca gtctttttct
     1921 tattaaagct agcttttggt gcaacttctt catattgaga taaagcacct gtagattctg
     1981 ctatatctaa atcttctaaa ggcaaagtag caaatttgtc ttgagcaact tcttcacaag
     2041 ctgtatttat tgcaatttca tctattataa catcatcact aggtaaggaa acagttactt
     2101 cgtcttctac aaccattaat atttcgttct gaactaaact agattttgtc tgatttttat
     2161 ctttagcttt tggttcgcta acaacttgtg ctatttgttc agcaactaag ctatcttcac
     2221 tttcaacatc tgaaaatata ataggacttg atgtttcttg aacaatttga gtttgttttg
     2281 tagttttatg cgaatttatt ttactaataa ttaaaaatat tcccgaagca ataaacaaaa
     2341 ttgctatcat agcagcatac ttataatatg attgtaaaga tatctttcgc ttttttctat
     2401 aaatatttct agtagaaatg attttactta tttcgatatt taatttttgt ttaactttaa
     2461 aataatcttc tgggtcttct aatcttaaaa ttccttctac aaaatctgca caaatttcac
     2521 agtttagaat atggttttct actactgcca attcctcatc gttcaattca tcattaacga
     2581 atttaactag aatttcttcc gaaaaacaat ctgaattatt taatatgtca tgtttattat
     2641 ctttcattct tttccatgca aatttttaaa tttctttttc cattttgcaa aaaactcttt
     2701 acttctttta aactaaagcc tgtaatttga gcaatttcaa catatgattt tgattgtaaa
     2761 taaaacaatt cgacacaaag tttttgttct tgtttaagta aagagatgca gtttttaagt
     2821 ttttcaattt ctatttcttt tttttcacta cttagatgaa aatcttcatc tttttccata
     2881 aaaattttca aatcacttga ataattcgca aataattttt cttcttgctt caaatttcta
     2941 aaatataaca aacaatgatt tcgacataca gaatgtaacc aagatttaaa atttgaaact
     3001 tcataaatta ataatttatc aattaagtct ccaaaaatta ttaaaactgc ctcttgagcc
     3061 aattctttgt tttttaaata ttttaaacaa atggaaaagc aaaaacccga atatctttta
     3121 aataactcgc caacaatttc tttgtttttt gactttttat atttttcaat taattctaag
     3181 tcgctaagta ataatatatt gtttggcaat ttttgcatca attctaaata gttcgactgt
     3241 caaaaataca ttaaattgta aataatacaa tataattttt aaagttatta ttcaacaaca
     3301 aatttactca ccaatatatt atcggacaaa tagatttttg cgaagtaaac tcctgaactc
     3361 aaagcactta tatcaacttt tgcaatatta ttagaaacag ttttataatt tacaacttgc
     3421 cctgaaatat ttaaaatttc aacatactca atattttctt ccgaaacaat atttaaaata
     3481 cttgttgcag gatttggata aatttgtatt tcagtaatat taaaatcttc tgtattggta
     3541 aaaaaacaaa aattataatg gggattttga taataaaaat tatcattttt ataagcacaa
     3601 aacaaatgat ttggtcctcc tgttatcgga atttgttcac gtccacaatc atgaaatcca
     3661 ttcaacccac caacaccttc aatataaagg tctggaaagt caaaataaca tacatttaaa
     3721 agatagtgct ttcggctttc accatttatt tcagcataat aaattgaatc tactatcata
     3781 aaagtatcgc aaggttctga catgccccca caatacaaat catataatga acaatcagcc
     3841 atcaaaaaag gatttctaaa atttaaggta tctccttttt cgacataata atcataaatt
     3901 aagccttcat gtccatataa atttctggca taaacttttc tgtcatctgt ttctcttaaa
     3961 aatccaataa gttgtttgtt aatcaaggga tgatattcta cgtgactttc tgattcatac
     4021 agttttttat atgtctgttc tccgataaca gtatcttctc ctatgatttc gtaatgagtg
     4081 ctcgtaatta tatttgtacc acatattgaa gatccaagac taaaataatt tcctgtttca
     4141 actattggaa catattcttg cgacgaacaa atgtttgcaa ttgttataaa taaagacaaa
     4201 caaagtattt ttattttttt catgatattt aatttttaaa aattattatt caacaacaaa
     4261 tttacgcact aatacattat ctaaaaaata aatttttgca aagcaaactc ctgaactcaa
     4321 agcacttata tcaacttttg caatattatt agaaacagtt ttataattta caacttgccc
     4381 tgaaatattt aaaatttcaa catactcaat attttcttcc gaaacaatat ttaaaatact
     4441 tgttgcagga tttggataaa tttgtatttc agtaatatta aaatcttctg tattggtaaa
     4501 aaaacaaaaa ttataatggg gattttgata ataaaaatta tcatttttat aagcacaaaa
     4561 caaatgattt ggtcctcctg ttatcggaat ttgttcacgt ccacaatcat gaaatccatt
     4621 caacccacca acaccttcaa tataaaggtc tggaaagtca aaataacata catttaaaag
     4681 atagtgcttt cggctttcac catttatttc agcataataa attgaatcta ctatcataaa
     4741 agtatcgcaa ggttctgaca tgcccccaca atacaaatca tataatgaac aatcagccat
     4801 caaaaaagga tttctaaaat ttaaggtatc tcctttttcg acataataat cataaattaa
     4861 gccttcatgt ccatataaat ttctggcata aacttttctg tcatctgttt ctcttaaaaa
     4921 tccaataagt tgtttgttaa tcaagggatg atattctacg tgactttctg attcatacag
     4981 ttttttatat gtctgttctc cgataacagt atcttctcct atgatttcgt aatgagtgct
     5041 cgtaattata tttgtaccac atattgaaga tccaagacta aaataatttc ctgtttccac
     5101 tattgggaca taatcttgtg acgaacaaat gcttgctatt gttacaaata aagacaagca
     5161 aagtattttt agatttttca tgtaagtaat ttttaaaatt taaccttt
//
LOCUS       DAOPNX010000126         4870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_18943_length_4870_cov_7.707373, whole genome shotgun sequence.
ACCESSION   DAOPNX010000126 DAOPNX010000000
VERSION     DAOPNX010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18943_length_4870_cov_7.707373"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PKN53_10280"
     CDS             complement(<1..692)
                     /locus_tag="PKN53_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89376.1"
                     /translation="MKNLKILCLSLFVTIASICLSQDYVPIVETGNYFSIGSAGGGNP
                     NVTSTYYEIIGEDTIIEEQTYKKLYKSESGVEYHPLVNKQLVGFIRETDDRKVYIRSL
                     AGQEGLIYDYYVEKGDTLDFRNPCVMLHQSLYDFFHMEDQEICDTVAIVDSVYYAEIN
                     GESRKHYILNAYGSGLNACTSVSNLYIEGIGSLNGLYNCGYEHLLLVGGRDYVLCAYK
                     NGDLYYDNPNYD"
     gene            992..1519
                     /locus_tag="PKN53_10285"
     CDS             992..1519
                     /locus_tag="PKN53_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89377.1"
                     /translation="MKTKTLIILIIAMFCFTAIQAQDVEKDVQRIRIYYNILKAEIES
                     ETNELICFYVEDNKFERQKEGEAYTNTKIYFYYTDTPDGVDLKMIIFESKRGIFSTYA
                     EYMFDEGSLSFGFKQHNEDASKAERLYQQNGRVVRYSVGNVDKDIEESEYKIAMFNKT
                     ASQMLTVFNSYMLRD"
     gene            1523..2083
                     /locus_tag="PKN53_10290"
     CDS             1523..2083
                     /locus_tag="PKN53_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89378.1"
                     /translation="MKTKRFILLVVLTLGLFSFPRIYSQDVEKCIQAIREDYKTVKSQ
                     IASLQKDGYAGKFYCLHTIDNKYGKSYSAVGEYKEEIWFYYDYNNEQEDDYKPKLRMV
                     VGTTKSADRSIYFETLFSESGEILFAFEQSDNMAKRLYYNKGQIIRYSENNVDQKIDE
                     ITDEIIWMSRTAEERKLRFEYFYAVE"
     gene            2085..2672
                     /locus_tag="PKN53_10295"
     CDS             2085..2672
                     /locus_tag="PKN53_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89379.1"
                     /translation="MKTKTIYLLAIVFLIASCAGKQQDKTTTEAEQVKTTENEVQTES
                     SEDLNSEDTETITTEVELQNSTELSIEDKKKFLGTWKAEGDNPYTVFTLEFSADNKGE
                     ISAKYYDGGPFEVNFTCNFISSNKAELYFDYVAGSISFNEITDANATNANKNNKVKII
                     ECKLINEKEIKVNTFDSDKITHVPQSKNLSLFKFE"
     gene            2683..2913
                     /locus_tag="PKN53_10300"
     CDS             2683..2913
                     /locus_tag="PKN53_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89380.1"
                     /translation="MGKTKKELEELEEIKRLKAETEALKKAYAELSFHHKCSQTVLEV
                     ADELFDLGLQEKYKEELAIAKEQKKKQDENKK"
     gene            complement(3116..4255)
                     /locus_tag="PKN53_10305"
     CDS             complement(3116..4255)
                     /locus_tag="PKN53_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009230862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOD89381.1"
                     /translation="MGIFNKLRNEFIDIIEWLDPSNDIIVHRFERYQNEIKMGAKLTV
                     RESQVAVFVNEGQIADVFQPGMYTLQTQNMPILSTLKGWKYGFNSPFKAEVYFVNTKK
                     FLDNKWGTPNPVMLRDAEFGPVRIRAFGVYEFRVVDAAKFIKDVVGTDGSFTSDQVTN
                     QLRNIIVTRFTDAVGEAKIPVLDMAANYNEFSEKVAGVIIDEYREYGLEMTKFLVSNI
                     SLPPNVEEALDKRSSMGIIGNMNQYTQFQAANAMEAAATNPGGGTASEGMGMGMGFAM
                     AGQMMNAMNQGHAQQQQPQQQAGGAVPPPMPQALTFYAVINGAQAGPYDMNVLQQMVA
                     NGSINKDTLVWKQGMANWIAAGQVPELTGLFGAVPPPPPPPPPVG"
     gene            complement(4259..4603)
                     /locus_tag="PKN53_10310"
     CDS             complement(4259..4603)
                     /locus_tag="PKN53_10310"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOD89382.1"
                     /translation="MIKTYINYEQLKNRTGDDKELIYDFLEVLKSELENYEQNFETAF
                     LNNDKEALLSAIHKFKGAIGIFGFSNIYDKLVFIENELKKNISINCFYNEKNHIFISV
                     KKHIDELEKNIT"
BASE COUNT         1733 a          831 c          722 g         1584 t
ORIGIN      
        1 aaatcataat ttggattatc ataatataaa tcaccatttt tataagcaca taatacataa
       61 tcacgccccc ctacaagcaa aagatgctca tacccgcaat tatataatcc attcaagctt
      121 cctataccct caatataaag atttgaaaca gacgtacatg cattaagacc actaccataa
      181 gcatttaaaa tataatgttt tcgactttcg ccatttattt cagcataata aacagagtca
      241 actattgcta cagtatcaca gatttcctgg tcctccatat gaaagaaatc atataatgat
      301 tgatgcaaca tcacacaagg atttctaaaa tctaaagtat ctcctttttc aacataataa
      361 tcataaatta aaccttcttg tccagccaaa cttctaatat acacttttct atcgtctgtt
      421 tctctaataa atcctactag ttgtttgttt actaaaggat gatattctac accactttct
      481 gatttgtaca gttttttata agtttgctcc tcaattatag tatcttctcc tataatttca
      541 taataagtag atgtaacatt cggattacct ccacctgcac ttccaatact aaagtaattt
      601 cctgtttcaa ctattggaac ataatcttgt gataaacaaa tgcttgctat tgttacaaac
      661 aaagacaagc aaagtatttt tagatttttc atgatattta atttttaatt aaataagtta
      721 ataatatttg atttttattg cctgtgtcct tttgcaaggc gtttaaggtt taacaaacca
      781 acttcacaaa gctttagctt aatctaattt ttataatagt acaaatataa gcaaaatatt
      841 tcacataata acatttttct taaaataaat atactcacaa ctttattaca caaacataca
      901 cgcacttttt acaaatacaa agttgtaaat tttactttaa ctcacacttt aaacttggtt
      961 tttatttata aacatataat aaaataaaac aatgaaaaca aaaacactta taatccttat
     1021 aattgcaatg ttttgcttta cagcaataca agcacaagat gttgaaaaag atgtacaaag
     1081 aattcgtatt tattacaaca ttctaaaagc agaaattgaa agcgaaacta atgaattaat
     1141 ttgcttttac gtggaagaca ataagtttga gcgacaaaaa gaaggcgagg cttatacaaa
     1201 cactaaaatt tatttttatt acacagatac tccagatggt gtagatttaa aaatgataat
     1261 ttttgaatct aaacgtggaa ttttttctac atatgcagaa tatatgtttg atgaaggctc
     1321 tttgtctttt ggttttaaac aacataacga agatgcaagc aaagcagaac gcctttatca
     1381 acaaaatggc agagttgtta gatattccgt aggaaatgtt gataaagata ttgaagaaag
     1441 tgaatataaa atagctatgt ttaataaaac agcttcgcaa atgcttacag tttttaactc
     1501 ttatatgtta cgcgattaaa ttatgaaaac aaaacgattt attttattag tagtattaac
     1561 acttggacta ttttcttttc ctcgaattta ctctcaagat gttgaaaaat gtattcaagc
     1621 aatacgagaa gattataaaa cagtgaaatc tcaaattgca agtttgcaaa aagatggata
     1681 tgctggtaaa ttttactgtc tgcataccat agacaacaag tacggaaagt catattcggc
     1741 agttggcgaa tacaaagaag aaatttggtt ttattacgat tacaataacg agcaagaaga
     1801 tgactacaag ccgaaattac gtatggttgt tggaacaaca aagtctgctg atagatctat
     1861 ttattttgaa actcttttta gtgaaagtgg agagatttta tttgcttttg aacaaagcga
     1921 taatatggca aaacgcttgt attacaacaa agggcaaatt attcgataca gcgaaaataa
     1981 tgttgatcaa aaaatagatg aaattacaga tgaaattatt tggatgtcgc gcactgcaga
     2041 agagcgtaaa cttagatttg aatattttta tgccgtagaa taatatgaaa acaaaaacaa
     2101 tttacttatt agcaatcgtg tttttaatag cttcatgtgc tggaaaacaa caagacaaaa
     2161 caactacaga agcagaacaa gttaaaacta ccgaaaacga agttcaaaca gaaagttctg
     2221 aagacttgaa cagcgaggac actgagacaa taactaccga agttgaattg caaaactcta
     2281 cagaattgag tattgaagac aaaaagaaat ttttaggcac atggaaagca gaaggcgaca
     2341 acccttatac tgtttttact ctcgaattta gtgcagacaa taaaggtgaa atttcagcaa
     2401 aatactatga cggcggtcct tttgaagtga attttacttg caattttatt tcaagcaaca
     2461 aagcagaact ttattttgat tatgttgcag gttcaatatc tttcaacgaa attacagatg
     2521 ccaatgctac aaatgcaaac aaaaacaata aggtaaaaat tatcgaatgt aaattgataa
     2581 acgaaaaaga aataaaagta aatacttttg atagcgataa aattacccat gttcctcaaa
     2641 gcaaaaattt gtctctgttt aagtttgaat aaaaaaatta ttatgggaaa aacaaaaaaa
     2701 gaattagaag aattagaaga aataaaacgt cttaaagcag aaaccgaagc cctcaaaaaa
     2761 gcttatgcag aactttcttt tcatcataaa tgttctcaaa cagttttaga agttgctgac
     2821 gaattatttg atttaggctt acaagaaaag tacaaagaag aattagccat tgccaaagaa
     2881 cagaagaaaa aacaagacga aaataaaaaa taaaatacat acgctcattt cggctacgct
     2941 caatgagcgt tattcaatca aggtgtttta gaagtgttca aatgcaaggc atctgagatt
     3001 gaaaacgaag gagcgtacca gaagcgatgc actgagcaaa gccgaagtgt acgtgactga
     3061 gtttgaatat cgaaagcaac gtagtagttg gacacttata aaacaccgtt catatttatc
     3121 caacaggagg aggtggtggt ggaggtggcg gcacagctcc aaacaaacca gtcaactctg
     3181 gaacttgtcc tgcggctatc caatttgcca ttccttgttt ccaaacgaga gtatctttat
     3241 tgatactacc atttgctacc atttgttgta atacgttcat atcgtatggt ccggcttgcg
     3301 caccattaat aactgcataa aaagtaagtg cttgaggcat tggtggtggt actgcacctc
     3361 cagcttgttg ttgtggttgc tgttgttgag catgaccttg attcatagca ttcatcattt
     3421 gtcctgccat tgcgaagccc attcccattc ccattccttc ggaagctgta cctcctccag
     3481 gattagttgc agctgcttcc atagcatttg ctgcttggaa ttgagtatat tggttcatat
     3541 tgccaataat tcccatactt gaacgtttgt caagagcttc ttcaacattt ggtggtaaac
     3601 taatgttaga aactaagaat ttagtcattt ctagaccata ttctctatac tcgtcaataa
     3661 ttacacctgc aactttttcg gaaaactcat tataatttgc agccatatcc aaaactggaa
     3721 tttttgcttc tcctactgca tcggtaaaac gagtaactat aatatttctc aactgattag
     3781 ttacttggtc agaagtaaaa ctaccgtctg ttccaacaac atctttaata aattttgctg
     3841 cgtcaacaac tctaaactca taaacaccaa aagctctaat tctaactggt ccaaattctg
     3901 catcacgaag catcacagga tttggagttc cccatttatt atctaaaaac tttttagtat
     3961 taacaaagta aacctctgct ttaaatggag aattaaaacc atatttccaa ccttttagag
     4021 ttgacaagat aggcatgttt tgggtttgca aagtgtacat tcctggctga aaaacgtctg
     4081 caatttgtcc ttcattaaca aaaactgcaa cttgcgattc tcttacagta agttttgcac
     4141 ccattttaat ttcgttttga taacgctcaa aacgatgcac aatgatatca ttacttgggt
     4201 ctaaccactc aataatatca ataaattcgt ttcttagttt attaaatatt cccataattt
     4261 aagttatatt tttttcgagt tcatcaatgt gtttttttac ggatataaag atatgatttt
     4321 tttcgttata aaaacaattt attgaaatat tttttttaag ctcattttct ataaaaacta
     4381 acttatcata aatattgcta aaaccaaata tcccaattgc gcctttaaat ttatgtatag
     4441 ccgacaacaa tgcttcttta tcattattta aaaatgcggt ttcaaaattt tgctcgtaat
     4501 tttctaattc cgattttaaa acctctaaaa aatcatagat taattcttta tcatcgcctg
     4561 ttctattttt taactgctcg tagttgatgt aagtcttaat cattttgata tttcatttac
     4621 caacaaaaaa gttttcaaag gcggtgtctc ataagtgtca aactgctgac ctgagttcat
     4681 aaagttaaag gttcataaag tttttaaagt cggttttata acaaaatgtg tcatttttct
     4741 tgaaggcgtc agttcctttt aacacttcaa ctacgttcag tgcatcgctt ttaactttta
     4801 acttttaact caagtgtacg ctccttcgtc ctcaatttca gcgccttgca ttttaacact
     4861 tctgaaacac
//
LOCUS       DAOPNX010000127         4718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_19749_length_4718_cov_5.545143, whole genome shotgun sequence.
ACCESSION   DAOPNX010000127 DAOPNX010000000
VERSION     DAOPNX010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19749_length_4718_cov_5.545143"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="PKN53_10315"
     CDS             complement(<1..647)
                     /locus_tag="PKN53_10315"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOD89383.1"
                     /translation="MAGHSKWSTIKRKKGALDAKRSKMFSKFSKEITIAVKEGGGHDP
                     DYNSRLRIAIQNAKGVNMPKDNIERAIKRGDQDDANFQEVSFEGYGSHGIAIFVECLT
                     DNNNRTVSSVRAAFNKFNGTLGTNGSLSFLFDRKGVFDFAKPEDRDFDEIQMELIDAG
                     AEEIEVEDGNVNVVCSIEDFGNMSKKLEELNIEVESAELQRIPNDTKTLSVEEAKS"
     gene            863..2128
                     /gene="metK"
                     /locus_tag="PKN53_10320"
     CDS             863..2128
                     /gene="metK"
                     /locus_tag="PKN53_10320"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006281187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOD89384.1"
                     /translation="MSYFFTSESVSEGHPDKVSDQISDAILDEFLAFDPNSKVACETL
                     VTTGLVVVAGEVKSEVYIDVQETARKVINKIGYTKSEYRFDGNSCGILSAIHEQSPDI
                     NQGVVKGAGLHKEDQGAGDQGMMFGYACRDTEELMPLPLDLSHKILQVLAEIRRENKQ
                     MTYLRPDSKSQVTIEYSDDNKPIRIDTIVVSTQHDDFDADDDKMLNKIKEDVKNILLP
                     RVIKGLDAKTQALFKDDYILHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGG
                     AFSGKDPSKVDRSAAYAARHIAKNLIAAGVADEILIQLAYAIGVAEPVGVFCNTYGTS
                     KVNIPDSEIAKKIKKIFSLKPADIEARFNLRTPIYLETASYGHFGRKPEKIKKVFESP
                     YLGTKTIEVELFSWEKLDYVDTIKKEFGL"
     gene            2220..3374
                     /locus_tag="PKN53_10325"
     CDS             2220..3374
                     /locus_tag="PKN53_10325"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOD89385.1"
                     /translation="MEKYKRFIVGAIGVVLLVLFVYYFSNVVMWILISAFIAMLGHPI
                     VEFIRKIHIKKLRPPRWLASLITIIFMYGVIYLVFRLISPLVMTQVQEFQSLDIESIS
                     EGLEKPIKNLDDYIQRTPILNQPEFSTEDYVVEKINSTINLIDVGKLINNLGSTIANL
                     FISLFVITFTSFFFLKDRDLFDRFVLMFVPAKFEEKVQGALKTLRRLISRYLLGLVLE
                     MVLMCLLITLGLYLIGINLNLAITIGVIIAALNVIPYIGPWIGVFIGTILLITANLHL
                     DFSAEVVPKILMLCGIVLVSKIIDDIIFQPVIYSKSVKAHPLEILFVIMIAGNLYGVL
                     GMALAIPGYTVLRVIAKVFFDEYKFIHKLTKGIDTQPEKTKPKIKEDEDI"
     gene            3361..4617
                     /locus_tag="PKN53_10330"
     CDS             3361..4617
                     /locus_tag="PKN53_10330"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOD89386.1"
                     /translation="MKIYKFGGASIKDSQALNNIVETILSKEKDLWIVVSAIGKTTNS
                     LEKLLNSWFNYEIDKFSNLEAVINFHKNYIKESFKENSENVEKLIEPIYISLEKLISQ
                     KPDKDYDILYDEIVSYGELMSSLIFSEYLKVKGIDNTLLDARELIITDSNYRDAKVSW
                     LESQKKINEIDKKSSKIFVTQGFIGADVNNITTTLGREGSDYTASALAYLLDAESVTI
                     WKDVPGIMNADPSYFDFAEKLKELSYNEAIELAFYGAKVIHPKTIKPIENKNIPLYVK
                     SFVNPENEGTVIKKLNYKLNLIPVFILKQNQILISISPKDFSFIVEENMSKIFGLFAK
                     YKTKLNISQNSAVSFSASIDDNNRNLKKLIKELQDEFFVKYNSGLELITIRYYTEESI
                     DKMLKGRKILMEQRSRKTARFLVKMV"
BASE COUNT         1723 a          649 c          742 g         1604 t
ORIGIN      
        1 gacttagctt cttcaacact taatgttttt gtatcgttag gtattctttg aagttcggca
       61 ctttctactt caatatttag ttcttcaagt tttttgctca tatttccaaa atcttcaata
      121 gagcaaacca cattaacgtt tccgtcttca acttcgattt cttcggcacc tgcatcaata
      181 agttccattt gaatttcgtc aaaatctcta tcttcaggtt tagcaaaatc gaaaactcct
      241 tttctgtcaa aaagaaaact taaagaacca tttgtaccaa gtgtcccatt aaatttatta
      301 aaagctgctc taaccgagct tacagttcta ttattattgt ctgttaagca ttcaacaaaa
      361 atagcaatac catgagatcc gtatccttca aaactaactt cttgaaaatt agcatcgtct
      421 tggtcgcctc ttttaatagc tctctcaata ttatctttag gcatattaac acctttggca
      481 ttttggatag caattcttaa tcttgagtta taatctggat cgtgtccgcc accttcttta
      541 acagcaatag taatttcttt tgaaaattta gaaaacattt tagacctttt tgcatctaaa
      601 gcaccttttt tgcgtttaat tgttgaccat ttactatgtc cagccatatg tgtatattaa
      661 ttaatagtta tattgtgcaa aaataaaaaa aatatttctt tttttgtaag atttacaaaa
      721 acttttttaa ttttgcattg ttttactagt ggacggattt ggatgcttgc aaccacttaa
      781 aaaaaattgc taaaaacatc agtttcaatc attcaaatcc tcactattat aattttagaa
      841 tataaattta aaatatataa ttatgagtta tttttttaca tccgaatcag tttcggaagg
      901 gcatccagac aaagtttcag atcaaatttc agatgcaatt ttagacgaat ttttagcttt
      961 tgacccaaat tcaaaagtgg cttgtgaaac tcttgtaaca acaggattag tagttgttgc
     1021 tggtgaagta aaatcagaag tttatattga tgtacaggaa actgcacgta aagtaattaa
     1081 caaaattggt tacaccaaaa gcgaatatcg ctttgatgga aattcttgtg gaatactatc
     1141 tgctattcac gaacaatcac cagatataaa ccaaggcgta gtaaaaggag caggcttaca
     1201 taaagaagat caaggagccg gcgaccaagg tatgatgttc ggatatgctt gtagagatac
     1261 agaagaactt atgcctttgc ctttagattt atcgcataag attttgcaag tgcttgccga
     1321 aattagaaga gagaacaaac aaatgacata tttgcgtcca gattcaaaat ctcaagttac
     1381 tatagaatat tctgacgaca ataaaccaat aagaattgat actattgttg tctcaactca
     1441 acacgacgat tttgatgcag acgatgacaa aatgcttaat aaaataaaag aagacgtaaa
     1501 aaatattttg ttgccaagag taattaaagg tttagatgca aaaacacaag cattatttaa
     1561 agacgattat attttacacg taaatccaac aggaaaattt gttattggtg gacctcatgg
     1621 agatactgga ttaacaggaa gaaaaataat tgttgacact tatggtggca aaggtgcgca
     1681 tggcggtgga gcttttagcg gaaaagatcc atcaaaagtt gaccgatctg ctgcttatgc
     1741 tgcaagacat atcgcaaaaa atttaatagc tgctggtgtt gctgacgaaa ttttaattca
     1801 acttgcttat gctattggag ttgctgaacc agtcggagta ttttgcaata catacggaac
     1861 ttctaaagta aatattccag atagtgaaat tgcaaaaaaa atcaaaaaaa tatttagcct
     1921 aaaacctgcc gacattgaag ctagatttaa tctaagaaca cctatttatt tagaaactgc
     1981 ttcgtatggg cattttggaa gaaaacctga aaaaattaaa aaagtattcg aatctccata
     2041 tttaggaaca aaaactattg aggtagaact ctttagttgg gaaaaactag attatgtcga
     2101 tacaatcaaa aaagaatttg gcttataaat cacaaaattg ttaaccgctt gtttaaaact
     2161 aaaaaaaaat aagcggtttt ttttgatttt attgattatt ttcgtaaaaa ttttttgaaa
     2221 tggaaaagta taaaagattt atagttggag caatcggtgt agtgttacta gtgcttttcg
     2281 tttactattt ttcaaacgtt gtaatgtgga tattaatttc tgcttttatt gcaatgttag
     2341 gacatccaat agtggaattt atacgaaaaa tccacataaa aaaacttaga ccaccacgct
     2401 ggttagcatc tttaataaca attattttta tgtatggtgt aatttatctc gtatttagac
     2461 taatttcacc attggtaatg acacaagttc aagaatttca atccttagat attgaaagta
     2521 tttcagaagg tttagaaaaa ccaattaaaa atcttgatga ttatatacaa agaacgccga
     2581 tattaaatca acctgaattt tctacagaag attatgtggt agaaaaaatt aactcaacaa
     2641 taaatttaat tgatgttgga aaattaatca ataatcttgg tagtactatc gcaaatcttt
     2701 ttatatctct atttgtaatt actttcacaa gttttttctt cttaaaagat agagatttat
     2761 tcgatagatt tgttttgatg tttgtaccag ctaaatttga agaaaaagta caaggagcat
     2821 taaaaacatt gcgaagacta atatctagat atttgttagg actagtttta gaaatggttt
     2881 taatgtgtct tcttattaca ttgggattat atcttattgg aataaacttg aatttggcta
     2941 ttacaatagg tgttattata gctgctctaa acgtgatacc ttatatcggt ccttggattg
     3001 gtgtttttat aggaacaatt ttgcttatta cagccaattt gcacttagat tttagtgccg
     3061 aagttgtacc aaaaatttta atgctatgtg gaattgtttt agtatcaaaa attattgacg
     3121 atattatttt tcaacccgtt atttattcaa aaagtgtgaa ggcgcatcca ttagaaatac
     3181 tttttgtaat tatgattgct ggaaatttat atggagtttt aggtatggcg ttagctatcc
     3241 caggttatac cgtattaaga gttattgcaa aagtattttt tgatgaatat aaatttatac
     3301 ataagttaac taaaggaata gatactcaac ctgaaaaaac taagccaaaa attaaagaag
     3361 atgaagatat ataaattcgg aggtgcttct attaaagatt cacaagcatt aaataatatt
     3421 gttgaaacaa tactttcaaa agaaaaagac ttgtggatag ttgtatcagc tattggtaaa
     3481 actaccaatt cactcgaaaa attgttaaat tcatggttta attatgaaat agataaattt
     3541 tcaaatttag aagccgttat taatttccat aaaaattata ttaaagaaag ttttaaagaa
     3601 aattctgaaa atgtagaaaa attaattgaa ccaatttata tttctttaga aaaattaata
     3661 tctcaaaagc cagataaaga ttatgatatt ttatatgacg aaatagttag ttatggagaa
     3721 ttgatgagtt cactaatttt tagcgaatat ttaaaagtaa aaggaattga taatacatta
     3781 cttgatgcaa gagaattaat tattacagat tcaaattatc gtgatgctaa agtaagctgg
     3841 ttagaatctc aaaaaaagat aaatgaaata gataaaaaat caagcaaaat atttgttaca
     3901 caaggtttta ttggtgctga tgtaaataat ataactacga cattaggtcg cgaaggttct
     3961 gactatactg cttctgcttt agcttattta ttagatgccg aaagtgtaac tatttggaaa
     4021 gacgtaccag gcattatgaa tgcagaccca agttattttg attttgccga aaaacttaaa
     4081 gaactatcat ataacgaagc tattgaactt gcattttacg gtgctaaagt tattcaccca
     4141 aaaactatta aacctatcga aaataaaaac ataccactat atgttaagtc ttttgtaaat
     4201 cccgaaaatg aaggaactgt cataaagaaa cttaattata aattaaacct gatacctgtt
     4261 tttatcttga agcaaaatca aattttaata tcaatttcgc ctaaagactt ttcgtttatt
     4321 gttgaagaaa atatgagtaa aatttttggt ttgtttgcaa aatataaaac taaattaaat
     4381 atttctcaaa actcggcagt gagcttttct gcttctattg atgataataa tcgaaattta
     4441 aaaaaactaa taaaagaatt acaagacgaa ttttttgtga aatataattc aggacttgaa
     4501 ctaattacaa taagatatta taccgaagaa tcgatagata agatgctgaa aggtagaaaa
     4561 attttaatgg aacaaagaag tagaaaaaca gcaaggtttt tagtgaaaat ggtttagtta
     4621 acatattttt attttataaa aatggaaata atttcgattt tgctaatatc tatagggctt
     4681 tcaatggata gccttgcgat ttcaatagcg acagcttg
//
LOCUS       DAOPNX010000128         4167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_23161_length_4167_cov_6.973979, whole genome shotgun sequence.
ACCESSION   DAOPNX010000128 DAOPNX010000000
VERSION     DAOPNX010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23161_length_4167_cov_6.973979"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..525)
                     /locus_tag="PKN53_10335"
     CDS             complement(166..525)
                     /locus_tag="PKN53_10335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOD89387.1"
                     /translation="MNLNNRVQLIGIIVNEPVIKTFGTGNKVARFTVETTDVYKQGEK
                     LVKETLRHNVVAWGSLAEMVSNSLSKSSEIALEGKLMRRSYTDHRGDTQYITEVVLTT
                     IVQSSFMKDLSIKTKIA"
     gene            complement(537..938)
                     /locus_tag="PKN53_10340"
     CDS             complement(537..938)
                     /locus_tag="PKN53_10340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOD89388.1"
                     /translation="MNFKFEKLVIWKSAMEYGELINKTVLSFPKDEAFYLINQFRKAA
                     DSIALNIAEGAIVQSNPDQIRLTSYSIRSVAEVVTCLYKAKNRNFIDDELFDTAYQDA
                     HKLLKMLVAFKSKLELINKNRMEKQNSENNQ"
     gene            complement(1013..1345)
                     /locus_tag="PKN53_10345"
     CDS             complement(1013..1345)
                     /locus_tag="PKN53_10345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOD89389.1"
                     /translation="MNLVNNVTLIGNLGADPKIREFENGNMVANFSIATKEYYREKDE
                     FKSKTYWHNIVAWGNAAKKVQDKCVKGSEVVLNGKLTNRSYEDSKGVKHWVYEVVVNE
                     IICRPKSA"
     gene            complement(1383..1757)
                     /locus_tag="PKN53_10350"
     CDS             complement(1383..1757)
                     /locus_tag="PKN53_10350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOD89390.1"
                     /translation="MNLKNRVQLIGNLGAAPKIKELKEGRKMARFSVATNSVEKRNGK
                     FEKTVNWYNVTAWGKTAEIVEQKLSKGSEVIIEGKLVSNSYTDLNGNVRKFYEVLAES
                     LLYRNIKTAIAKAENSENKTRA"
     gene            2044..2940
                     /locus_tag="PKN53_10355"
     CDS             2044..2940
                     /locus_tag="PKN53_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HOD89391.1"
                     /translation="MYYRRKILLALLSVFDGQLTAKQFQKYLFLFTRKQEIKSFDFVP
                     YRYGCFSFQANQDIATLAKYGYCTIAENRKVRFFELTKDDDFFNTLKPEDRKSLLEVK
                     AQFGNLSQSDLIRYTYRQYPYYAIKSVIAKDLLNSTELNSIELQKRHYPEKQLFSIGY
                     EGISLEAYINKLIINDVNVLCDVRKNAYSQKYGFSKKTLELACKSVDIEYVHIPDLGI
                     ISNKRKELSSQEDYDDLFREYEKTTLKENRASLLAIRSLLDKGKRVALTCFEKDPKQC
                     HRTCVANALMQLPETNYKFKNL"
     gene            3121..3777
                     /locus_tag="PKN53_10360"
     CDS             3121..3777
                     /locus_tag="PKN53_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89392.1"
                     /translation="MDIVRNTKDFRKESYRPTDLDKEIIIGKTVPTTKNWALRKEIAL
                     KNVYTDMSVLIEEAKDKNIGTSLAVLKPKKVIDFIWESCEREWNKKKLDAIIAKQAQG
                     SLFDTEETKRIFKVAKKLPYKFFYVFTTEDDKERTMMVEDWELGALYWNCLKNANDNE
                     ELACQKVREKYFDYMVNKCDLHFFLGTTQKFHNVGLNPFIIIGVFYPLKEDATQLSLF
                     "
     gene            complement(3857..4135)
                     /locus_tag="PKN53_10365"
     CDS             complement(3857..4135)
                     /locus_tag="PKN53_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89393.1"
                     /translation="MLRSNSRYNITNQGIISNWINFIEYHYIPARLILGFEYKNILNS
                     PISCEILATPSYMFNIYQKTDAIMLSNNTNDLKILNLILGIKYTFKQI"
BASE COUNT         1374 a          630 c          634 g         1529 t
ORIGIN      
        1 tcataagtgt caaactgctt cgttgctttc aatattcgga ctcagtcacg tacttatggt
       61 acgctctttc gtcctcaatt tcagcgcctt gcattttaac acttctgaaa caccttgaaa
      121 gtttattgtt ttaaaatttg ttttgagaca gcctcctttt taagtttaag caatttttgt
      181 ctttatcgac aaatctttca taaatgaact ttgcacaatt gttgtcaaga caacttctgt
      241 tatatattga gtatcgcctc tatggtcggt ataagaacgt ctcataagtt ttccttcaag
      301 ggcaatttcc gaagatttag acaaagaatt gctaaccatt tcggctaaac taccccaagc
      361 aacaacattg tgtcgtaaag tttctttcac tagtttttct ccttgtttat acacatctgt
      421 ggtttctact gtaaaccttg ctaccttatt gcctgtgcca aatgttttaa taacaggctc
      481 gtttacaatt attcctatta gttggactct attattcaaa ttcataatta aaaaatttat
      541 tggttatttt cagaattttg tttttccatt ctattcttat ttataagctc aagtttgctt
      601 ttaaaagcaa ctaacatttt taataattta tgagcatctt ggtacgctgt gtcaaaaagt
      661 tcatcgtcga taaagtttct gttttttgct ttgtataaac aagtaaccac ttcggcgaca
      721 gaacgaatag aataagaggt taatcttatt tgatcaggat ttgattgcac aatagctcct
      781 tcggcaatat ttaatgctat ggaatcagca gctttgcgaa attgatttat taaataaaat
      841 gcttcatctt ttggaaaact taaaacagtt ttatttatta attccccata ttccattgct
      901 gatttccaga taacgagttt ttcaaattta aaattcataa tgatttaatt taattaatgt
      961 taaaaattat ggtgtttcag aagcagagaa cttctgaaac accacaaaat tattatgcag
     1021 atttaggtct gcaaataatt tcattaacta ccacttcgta aacccaatgt tttacgcctt
     1081 ttgaatcttc gtagctacga ttggttaatt ttccgttaag cacaacttcg ctacctttca
     1141 cacatttgtc ttgcactttt ttagcagcat ttccccatgc tacaatattg tgccaataag
     1201 tttttgattt aaactcatct ttttctctgt aatactcttt tgtggcaata gaaaaatttg
     1261 ctaccatatt accattttca aattctctaa ttttaggatc tgcacctaag tttccgatta
     1321 aagttacatt gtttactaaa ttcataatac taaattttaa ctgttataca tttttaaatt
     1381 aattacgctc ttgttttatt ttcgctgttt tcagctttag caatagctgt ttttatgttt
     1441 ctatacaata aactttctgc taaaacttca taaaatttac gtacattacc gtttaggtct
     1501 gtgtaactat tacttactaa tttaccttcg ataataactt cgcttccttt tgatagtttt
     1561 tgttcaacta tttcggcagt tttgccccag gcagttacat tgtaccaatt tacagttttt
     1621 tcaaattttc cgtttctttt ttctacagaa tttgtggcaa ccgaaaaacg agccattttt
     1681 ctaccttctt ttagttcttt gattttaggt gctgcaccta agtttccaat taattgtaca
     1741 cgatttttta aattcataat attaaatttt aaaagttaaa cattaaattt ttgtcttgta
     1801 aaattttcaa aaactgagaa ttaaacttcg acgaatttgt cggtgcaaag atgtgggtat
     1861 aaaaatcccg tagtcggttt ttaaaggttt actttcgttt aatattcgtt tgtaagcgtt
     1921 tgtaagaaaa taatgagctg gtaatgagtg ggttagttgt cgagtgtttt tttagataaa
     1981 ttatagatat tttttgattt ttatttgtta tgaataattt tttgtatttt tgtaaagaga
     2041 aatatgtatt acagaagaaa aatattattg gctttgttaa gtgtttttga tgggcaactc
     2101 actgccaaac aatttcaaaa atacttattt ttgtttacac gtaagcagga gataaaatct
     2161 tttgattttg ttccatatcg ctatggttgt ttttcttttc aagccaacca agacattgct
     2221 acacttgcta aatacggcta ttgtacaata gcagagaata gaaaagttag gttttttgaa
     2281 ttaacaaaag atgatgattt ttttaatacg cttaaaccag aagatagaaa aagtcttctt
     2341 gaagtaaaag cgcagtttgg aaatttaagc caatcagact taattcgtta tacatatcgt
     2401 caatatccat attatgctat aaaaagtgtt attgccaaag atttgttaaa tagcacagag
     2461 ctgaactcta tagagttaca aaagagacat tatcctgaaa aacaactttt ttctattgga
     2521 tatgaaggta tttctcttga agcatatata aataaattaa taattaatga tgttaatgtg
     2581 ctttgtgatg ttagaaaaaa cgcttatagt caaaaatatg gtttttctaa aaaaactctc
     2641 gaattagctt gtaaaagtgt tgatattgaa tatgttcaca ttcccgattt gggaattatt
     2701 tctaacaaac ggaaagaatt aagtagtcaa gaagattatg atgatttatt tcgcgagtat
     2761 gaaaaaacta ctttgaaaga aaatagagct tcgctactcg ctattcgcag tttgttagat
     2821 aaaggtaaac gagtggctct tacctgtttt gaaaaggatc caaaacaatg tcacagaact
     2881 tgtgtagcaa atgcactaat gcaattaccc gaaacaaatt acaaatttaa aaacttgtaa
     2941 atttatggct cttactcgca ttcttatttc tgtaaaacat atcctacgct ttctgctact
     3001 tacgatgagt tagtttgcac agctggtttt aaagaagatg gttcgtgggt tagaatatat
     3061 cctgttccat ataggaaatt aaaatatgcc gaccgttatg aaaaatggca atggatagaa
     3121 atggatattg tccgcaatac aaaagatttc agaaaagaaa gctatcgtcc tacagattta
     3181 gataaggaaa ttattattgg aaaaactgta cctactacaa aaaattgggc acttcgaaaa
     3241 gaaattgctc ttaaaaatgt ttatacagat atgtctgttt tgattgaaga agcaaaagac
     3301 aaaaatattg ggacatcttt agctgtactt aaaccaaaaa aagttataga ctttatatgg
     3361 gaatcatgtg aacgagaatg gaataagaaa aaacttgacg caattattgc aaaacaagca
     3421 caaggcagtt tgtttgatac tgaagaaacc aaaagaatat tcaaagttgc aaagaaacta
     3481 ccctacaaat ttttttacgt ttttaccact gaagatgata aagaacgtac tatgatggtg
     3541 gaggattggg aattaggcgc tttatattgg aattgcttaa aaaatgcaaa tgataatgaa
     3601 gagttagctt gtcaaaaagt gagagaaaaa tactttgact acatggtaaa taaatgtgat
     3661 ttacattttt ttcttggcac aactcaaaaa tttcataatg taggacttaa tccatttata
     3721 attatcggtg tgttttatcc tctaaaagaa gatgcaacac aactttcgct attttaagtg
     3781 tattactaaa ataaataagc ctctcaggcg cgtctttggc agcatacttg aggggcgttc
     3841 ttcattaata agtagtttaa atttgtttaa aagtatattt tattcccaaa attaaattta
     3901 atattttcag gtcgttagta ttgtttgaca acattatagc atctgttttt tgataaatat
     3961 tgaacatata tgacggagtt gccaaaattt cacaactaat tggagaattt aaaatgtttt
     4021 tatattcaaa acctaaaatc agtcttgcag gaatgtaatg gtattctata aaattaatcc
     4081 aatttgaaat aatgccctga tttgtaatat tataacgtga gttcgaccta agcacaaatc
     4141 attttcagtc tttcttcatc aactata
//
LOCUS       DAOPNX010000129         3984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_24525_length_3984_cov_5.496055, whole genome shotgun sequence.
ACCESSION   DAOPNX010000129 DAOPNX010000000
VERSION     DAOPNX010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24525_length_3984_cov_5.496055"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PKN53_10370"
     CDS             complement(<1..206)
                     /locus_tag="PKN53_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase family protein"
                     /protein_id="HOD89394.1"
                     /translation="MSGIYLHIPFCKSKCSYCNFCSIALLKQKTDFLKALENELQANS
                     KNINEIEFSSIYFGGGTPSILNVIT"
     gene            complement(236..1705)
                     /locus_tag="PKN53_10375"
     CDS             complement(236..1705)
                     /locus_tag="PKN53_10375"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOD89395.1"
                     /translation="MRFSLNKYKIIAFYTIAFFFIIFNSWIIIKEENLLFNLFPIALI
                     VFLAMFFALDKVLLFSVFLVPFSVPLKTFVPGLEFNIDLPTEPIFVGILFIFILKQLQ
                     DRYFDTKLLKHPVSIALYIYLFWKFVTVFTSTMPIVSLKAFIASLWFIVPFYFLISQL
                     FKKDKKKIYNFLWLYIIPFIIIIIYTLIKHSARGFTQSTANFIVNPFYNDHTSYGAVL
                     AMFIPVLIGFVFSKDLKKYVRGWSFVVLVLFIVALIFSYTRAAWVSMLVGLGVYVILK
                     LKIKLKYILTVAVVMLGLFFAFQEQIFMSLSGNRQDSSTDFSEHIKSISNITTDASNL
                     ERINRWNCAVRMFEEKPIFGWGPGTYQFNYAPFQFSYEKTIVSTNAGDYGNAHSEYLG
                     ELSESGLLGMLSFTALIIIIVVTGVRSYQRAKSKLIKTLSMSLLVGLITYLAHAFLNN
                     FLDIDKVAVPFWGFVAALVIIDLYINKEEEKQCVENKEQ"
     gene            complement(1702..2631)
                     /locus_tag="PKN53_10380"
     CDS             complement(1702..2631)
                     /locus_tag="PKN53_10380"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOD89396.1"
                     /translation="MENYFNTKKIFSLIIKWKWHLMSFVIIVAVCSAIFSSPWFIKPK
                     YTSSAVVYPANVIPVSDESESEQMLEILKSEDIKFAIIEAFDLYKHYEVEKGQPKSNW
                     KILKYYDANINIRKTPNDAIVISASDIDPQIASNIVDSIIKFYDVLVLNLNASKSREI
                     VNIYKNQMQLKLKEIDSLGLILKTFRTDYNLLDMEAQVKKYTEAITNGRSLEESRKVL
                     DNWREYGSEFHKTDSLFYYALKDYRLNKEVYDNALRDANKVQTYSHVISKPFPADKKS
                     YPIRWVIILLSTLGAFFAGVIIISFIEASKKTK"
     gene            complement(2697..3704)
                     /locus_tag="PKN53_10385"
     CDS             complement(2697..3704)
                     /locus_tag="PKN53_10385"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOD89397.1"
                     /translation="MNLVSEENNSFDLKNLLNLIFRYKLPLFIVGGVALVMAIIVCLI
                     IQPRYKSTVILFPSSSGSISQAIITESQQKKDILRFGEEEEVEQLMQVLMSSEIRNRI
                     IEKYDLFRHYGIDPNSKHANSRLYRAYNSNIDISRTQFMSIKIEVSDISADTAALIAN
                     DIASLVDSIIFKIQRERAYKAFVIVEREFIAQKEKIKNIEDSLSALSVLGVIDVKSQT
                     EMYSEQHAIAIANGNERAKVEIEKKLDVLASYGSTHSILKEQMFEEIKQLAVLEAKYR
                     EAKIDLEQDLPNTYIVSPAEVADKKHFPICWLVVSISVLSALVFSLIIIILFDRYIKK
                     K"
     gene            complement(3775..>3984)
                     /locus_tag="PKN53_10390"
     CDS             complement(3775..>3984)
                     /locus_tag="PKN53_10390"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOD89398.1"
                     /translation="PKDVKIDTIAMYLGAENQKAHYDSILANPPRRIIFNPGTYNPEF
                     QQLLKEKGVDVVNDCVLIMNSRGIY"
BASE COUNT         1410 a          630 c          483 g         1461 t
ORIGIN      
        1 gtaatgacat ttaaaattga tggtgtacct ccaccaaaat aaatacttga gaactcaatt
       61 tcgttaatat tctttgaatt agcttgtaat tcattttcta aggctttgag aaaatctgtt
      121 ttttgtttta ataatgctat agaacaaaag ttacaatatg aacacttact tttacaaaac
      181 ggaatatgca aataaatgcc tgacatttaa gtcttaaaat atattttgtt ataatttact
      241 gttctttgtt ttctacacac tgcttttctt cttctttatt gatataaagg tctattataa
      301 caagagctgc tacaaacccc cagaaaggaa ctgcgacttt atcaatatct aaaaagttat
      361 ttaaaaaagc atgtgctaaa tatgttataa gccctaccaa caaagacatg ctaagtgttt
      421 ttataagttt agactttgct ctttggtaac ttctaactcc tgtaactaca ataatgatga
      481 ttaatgctgt aaaacttagc attcctagca atccagactc tgatagttct cctaaatatt
      541 cgctatgggc attaccgtaa tctccagcat ttgtgcttac aatagttttt tcgtaagaaa
      601 attgaaacgg tgcgtaatta aattgataag ttccaggtcc ccagccgaat ataggttttt
      661 cttcaaacat tcttactgca caattccaac gatttattct ttctaagttt gacgcatctg
      721 tagttatatt acttatagat tttatatgtt ctgaaaagtc tgttgatgag tcttgtctat
      781 ttccagaaag cgacataaaa atttgctctt ggaaagcaaa gaacaaacca agcataacaa
      841 cagcaacagt aagaatatat tttagtttaa tttttaattt caagattaca tacacgccta
      901 gaccaactaa catacttacc caagctgctc ttgtgtacga aaaaattagt gctacaataa
      961 aaagtactaa aacaacaaaa ctccagcctc taacatattt ttttaaatct ttagaaaaca
     1021 caaatcctat taaaactggg ataaacattg ccaacaccgc cccgtatgat gtatggtcgt
     1081 tgtagaatgg attcacaata aagtttgctg tactttgagt aaaacctcgt gcagaatgtt
     1141 taattaatgt ataaattata attattataa aagggattat gtacagccac aaaaaattat
     1201 aaatcttttt cttatctttt ttaaaaagtt gagaaattag aaaataaaaa ggaactataa
     1261 accacaagct agcaataaat gcttttaacg atactatagg catcgtgctt gtaaaaactg
     1321 taacaaattt ccagaacaga taaatataca aagcaataga tactggatgt tttaaaagtt
     1381 ttgtgtcaaa atatctatct tgcaattgtt taagaataaa aataaacaaa attcctacaa
     1441 atattggctc tgttggcaaa tcgatattaa actccaatcc tggtacaaat gtctttaaag
     1501 gaacagaaaa tggtactaaa aaaactgaaa aaagcaagac tttgtctaaa gcaaaaaaca
     1561 ttgctaagaa gacaattagt gctattggga ataaattaaa taataaattt tcttctttta
     1621 taattatcca agaattgaat attataaaaa aaaatgcaat agtataaaat gctattatct
     1681 tatatttatt tagagaaaac ctcatttagt ttttttagaa gcttcgataa atgaaattat
     1741 tataacacct gcaaaaaatg ctcctaaagt tgatagtaat ataattaccc atcttatagg
     1801 atatgatttt ttatcagctg gaaatggttt tgaaattaca tgagaatatg tttgtacttt
     1861 attagcatct cttaaggcat tgtcatatac ttctttattt aatctataat ctttaagtgc
     1921 gtaataaaac aatgaatctg ttttatgaaa ctctgaacca tattccctcc aattatctaa
     1981 aacctttctt gattcttcta aactacgccc attagttata gcttcggtat atttctttac
     2041 ctgagcttcc atgtctaata aattataatc tgttctaaat gtttttaaaa ttaatcctaa
     2101 gctatcaatt tcttttaatt ttaattgcat ttggttttta taaatattaa caatttctct
     2161 agattttgaa gcatttaagt ttaataccaa aacatcataa aatttaataa tagaatcaac
     2221 aatatttgaa gctatttgag ggtctatatc gcttgcagaa attacaatag catcatttgg
     2281 cgtttttctt atattgatat tagcatcgta atattttaaa attttccaat ttgattttgg
     2341 ctgtcctttt tcaacttcgt agtgtttgta taaatcaaat gcttcaatta tagcaaattt
     2401 tatatcttca ctttttaaga tttccaacat ttgttcactt tccgattcat ctgatacagg
     2461 tataacattt gctggataaa ctacagcgct agaagtatat ttaggtttta taaaccaagg
     2521 cgatgaaaat attgcactac aaacagctac tattataaca aatgacatca aatgccactt
     2581 ccattttata atcaatgaaa aaattttttt tgtgttaaag tagttttcca taacttcgta
     2641 ttttggcaat tcaaatttac taattatttc gcttaaatta aacgaaaaat taatttttat
     2701 ttttttttta tatatctatc aaataatata ataattataa gcgaaaaaac aagagcagac
     2761 aaaacagaaa tactcacaac taaccaacaa ataggaaagt gttttttgtc tgcaacttct
     2821 gctggactaa ctatatatgt atttggtaaa tcttgctcta aatcaatttt agcttctcta
     2881 tattttgctt ctaatacagc aagttgctta atttcttcaa acatttgctc ttttaagatt
     2941 gaatgtgtac ttccataact tgctaaaaca tctaattttt tttcaatttc aacttttgct
     3001 ctctcattcc catttgcgat tgctatagca tgttgctcac tatacatttc tgtctgagac
     3061 ttcacgtcta taactcctaa tacacttaga gcagatagag agtcttctat attttttatt
     3121 ttttcttttt gtgctataaa ttctctttca acaataacaa aagctttata agccctttcg
     3181 cgttgtattt taaaaattat actgtcaact aatgaagcta tatcatttgc aattagagct
     3241 gctgtatcgg cactaatatc tgaaacttca atttttattg acataaattg tgttctagaa
     3301 atatcaatat tagaattgta agctctgtat aaacgactgt ttgcatgctt tgagttagga
     3361 tcaataccat aatgtctaaa caagtcatat ttttcaataa ttctatttct tatttcagac
     3421 gacattaaaa cctgcattaa ttgttcaacc tcttcttctt caccaaatct tagaatatct
     3481 ttcttttgtt gactttctgt aataatagct tgagaaatac ttcccgaaga actaggaaat
     3541 aaaattactg tagatttata tctaggctga attattagac atactataat tgccattacc
     3601 aaagcaacac caccaactat aaacaatgga agtttatacc taaaaattaa gtttaacaaa
     3661 tttttcaagt caaaactgtt attttcttct gataccaaat tcactgacta aaattatttt
     3721 agtggacaaa aatataaaat attttaatat aaacagtatt tttagaaatt tttattaata
     3781 aattccccta ctgttcatta tcaacacaca atcatttact acatctacac ctttttcttt
     3841 taatagttgt tgaaattcag gattataagt acctggatta aaaattattc ttcgaggcgg
     3901 attagctaat attgaatcat aatgtgcttt ttgattttca gcacccaaat acatagcaat
     3961 agtatctatt ttaacatctt tagg
//
LOCUS       DAOPNX010000130         3949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_24814_length_3949_cov_5.585516, whole genome shotgun sequence.
ACCESSION   DAOPNX010000130 DAOPNX010000000
VERSION     DAOPNX010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24814_length_3949_cov_5.585516"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2415
                     /locus_tag="PKN53_10395"
     CDS             <1..2415
                     /locus_tag="PKN53_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease domain-containing protein"
                     /protein_id="HOD89399.1"
                     /translation="HLKDGLQYDVACEYAGYRHSKSSLTKEELENKKLKDRLEILPKN
                     SLRNPVVEKILNQMVNVVNEVIDTYGKPDEIRIEIARELKKSAKEREEMTQQINKATN
                     AHKAYKDILEKEFGLTNVSRNDIIRYKLYLELEDNGYKTLYSNTYIPREKLFSKEFDI
                     EHIIPQAKLFDDSFSNKTIEKRSVNIEKSNMTALDYVASKDANSLSEYKLRVEKLHKS
                     GQISKTKYNKLLMSEAEIPSGFINRDLRDTQYIAKKAREMLNELVKFVVPTTGTITAR
                     LREDWQLIDIMQELNWDKYDQQGLTMFYNDKDGRQIGRIIDWSKRNDHRHHAMDALTV
                     AFTKHSFIQYLNNLNARVQKGIDEYFDLDMVELSDLPKEERSATIYAIEKKYLHRDKN
                     GKLRFNPPIPLNEFRAEAKNHLENILISIKAKNKVVTQNTNITKKLSGKNKKTQLTPR
                     GQLHLDTVYGSYQKPIIKEEQINGSFDESKIKTVTNNSFREALLKRLQEFDNDPKKAF
                     TGKNSPSNNPIFVDKEKTLQIPNKVKTLTFETEYTIRKAIDPDLRIDKVVDTGIKEIL
                     EKRLAEFDNDPKKAFVNLDENPIYLNKEKGITIKRVTIKGISNAIALRDKRDKDGNFI
                     LNENGEKQAIDFVNTGNNHHVAIYKKPKLNRKGEVQLDDKGEQVYELEEVVVSFFEAV
                     TRVNLGQPIIDKEYKQDEGWQFLFTMKQNEYFVFPNEKTGFNPNDIDLLNPENYNIIS
                     PNLFRVQKISTKNYMFRHHLETNVNETKELRDITWKNIRSTQNLVNIIKVRVNHIGQI
                     VAVGEY"
     gene            2426..3376
                     /gene="cas1"
                     /locus_tag="PKN53_10400"
     CDS             2426..3376
                     /gene="cas1"
                     /locus_tag="PKN53_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HOD89400.1"
                     /translation="MIKKTLYFGNPAYLSLKNKQLVIKMPEVEKTELPDSIKESAIVT
                     VPIEDTGVVVLDNKQITITHGAMEALLENNVALITCDNNHLPVGLMLPLCGNTTQNER
                     FTKQIEASVPLKKQLWQQTVQQKILNQAAVLKSVTGCEVGNMMRWAKDVRSGDPDNLE
                     GRAAVYYWRNLFPDIPNFTREREGMSPNNLLNYGYAILRAVIARSLVSSGLLPTLGIH
                     HHNRYNAYCLADDIMEPYRPYVDKLVIELMSSGIDCSEITKEIKQELLQIPTLEVIIG
                     KKRSPLMIASTQTTASLYKCFTGELRRVAYPVLELNGKNG"
     gene            3369..3701
                     /gene="cas2"
                     /locus_tag="PKN53_10405"
     CDS             3369..3701
                     /gene="cas2"
                     /locus_tag="PKN53_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOD89401.1"
                     /translation="MDRFSEYRIMWILVLFDLPTETKKERAAASKFRKQLIEDGFNMF
                     QFSIYTRHCASRENAEVHIKRVRRFIPEQGTVSILCITDKQFGNMEIFYGKKEKEPPS
                     GWQQLKLF"
BASE COUNT         1571 a          638 c          636 g         1104 t
ORIGIN      
        1 catttaaaag atggacttca atatgatgta gcttgcgaat atgctggcta tcgccattct
       61 aaaagctcac taacaaaaga agaattagaa aacaaaaaac taaaagatag acttgaaatt
      121 ttgccgaaaa acagcttacg aaaccctgtt gttgaaaaga ttctaaatca aatggttaac
      181 gtagtaaatg aagtaattga tacttatggc aaacctgacg aaataagaat cgaaatagcg
      241 agagagttga aaaaatctgc caaagagcga gaagaaatga ctcaacaaat aaataaagcc
      301 acaaatgctc ataaagcata taaagatatt ttagaaaaag agtttggact aacaaacgta
      361 agtcgaaacg atattattag atacaaactt tatttagaac tcgaagacaa tggctacaaa
      421 acactatatt caaacacata tattcctcgc gaaaaacttt ttagcaaaga atttgatatt
      481 gaacacatta ttcctcaagc taaacttttt gatgactcat tttcaaataa aactattgaa
      541 aaacgtagcg taaatatcga aaaaagcaat atgactgcat tagattatgt agcctcaaaa
      601 gatgctaact cgctatctga atataagtta agagtagaaa aacttcataa atcaggtcaa
      661 atcagtaaga ctaaatacaa caaactctta atgtctgagg cagaaatccc aagcggtttt
      721 ataaatagag acttacgaga tactcaatac atagcaaaga aagcccgaga gatgttaaat
      781 gagctagtaa aatttgttgt accaactaca ggtacaatta cagctcgttt acgtgaagat
      841 tggcagttga ttgatataat gcaagaactc aactgggata aatatgatca acaaggactc
      901 acaatgtttt acaatgataa agatggaaga caaattggca gaattattga ctggtctaaa
      961 cgcaacgacc accgccacca cgcaatggat gccctcacag tagcatttac taaacatagc
     1021 tttattcaat atctaaacaa tttgaacgca agagttcaaa aaggaataga cgaatatttt
     1081 gacttagata tggtagaatt atctgacttg cctaaagaag aacgttctgc aacaatttat
     1141 gctattgaaa aaaaatattt gcatcgtgac aaaaatggaa aacttcgctt taacccacct
     1201 ataccattaa atgaatttcg agctgaagca aaaaaccatt tagaaaacat tttgatttct
     1261 attaaagcaa aaaacaaagt tgtaacacaa aatacgaata ttacaaaaaa attgagcgga
     1321 aaaaataaga aaacacaact tacaccacgc ggacaactcc atttagatac tgtttatggc
     1381 agttatcaaa aacccattat aaaagaagaa caaattaatg gtagtttcga tgaaagcaaa
     1441 ataaaaactg taacaaacaa tagttttaga gaagctttac taaaacgatt acaagagttt
     1501 gacaatgatc ctaaaaaagc atttactggt aaaaactctc catctaataa tcctatattt
     1561 gttgacaaag aaaaaacatt acaaatacca aataaagtaa aaactttgac ttttgaaacc
     1621 gaatacacca tacgtaaagc aattgatcct gatttacgta ttgataaagt tgtagataca
     1681 ggaataaaag agatattaga aaaacgtctt gctgaatttg ataacgaccc taaaaaggct
     1741 tttgtaaatt tagatgaaaa tcctatttat cttaataaag aaaaaggaat tacaattaaa
     1801 agagtcacga taaaaggtat tagtaacgcc attgctctac gtgacaagcg tgacaaagat
     1861 ggcaatttta tacttaacga aaatggtgag aaacaagcta tagattttgt aaatacgggg
     1921 aataatcatc atgttgcaat ttataaaaaa ccaaaattaa atagaaaagg agaagttcaa
     1981 ttagacgata aaggcgaaca agtttacgaa ttagaagaag ttgttgtatc tttctttgaa
     2041 gctgtaacac gtgtaaatct aggacaacca attatagata aagagtacaa acaagatgaa
     2101 ggctggcaat tcctatttac aatgaaacaa aacgaatatt ttgtatttcc aaacgaaaaa
     2161 actggtttta acccaaacga tatagatctg ctaaacccag aaaactataa tattataagt
     2221 cctaatttgt ttagagtaca gaaaatttca acaaaaaact atatgtttag acatcattta
     2281 gaaaccaatg tcaatgaaac taaagaactt agagatataa cttggaaaaa tatacgttca
     2341 actcaaaatc ttgtaaatat tataaaagtt agggtaaacc atattggtca aattgtagct
     2401 gtaggggaat attaaaaata aaaatatgat aaaaaaaact ctctattttg gcaatcctgc
     2461 atatttaagt ttgaaaaaca agcaattagt aatcaagatg ccagaggtag aaaaaactga
     2521 attacctgat agcattaaag aaagtgctat tgttactgtc ccaatagaag acactggagt
     2581 agtagtgctt gataataaac aaattactat tactcatggt gctatggaag ctttattaga
     2641 aaataatgtt gctttaataa cttgcgacaa taatcattta ccagttggtt taatgcttcc
     2701 tctctgtggc aacactacac aaaacgagcg ttttactaaa caaattgaag cttctgttcc
     2761 tctaaaaaaa caactttggc aacaaactgt tcagcaaaaa attttaaatc aagctgctgt
     2821 attaaagtca gttacaggct gtgaagttgg caatatgatg cgttgggcaa aagatgttcg
     2881 cagtggcgac cccgacaatc ttgaaggacg agctgctgtt tattattggc gtaatctttt
     2941 tcctgatatt ccaaatttca cacgtgaacg cgaaggcatg tcgcccaata atttacttaa
     3001 ctatggctat gctattttga gagccgttat tgctcgaagt cttgtttcaa gtggactttt
     3061 acccacactt ggcattcatc atcacaaccg ttacaatgct tattgccttg cagacgatat
     3121 aatggaacca tatcgccctt acgttgacaa acttgtgata gaacttatgt caagcggaat
     3181 agattgctcg gaaattacaa aagaaatcaa acaagaatta ttgcaaatcc caactcttga
     3241 agtaataatt ggtaaaaaac gtagtccctt aatgattgct tctacacaaa ctactgcatc
     3301 tctttataaa tgttttactg gtgaacttag acgagtggct tatccagttt tagaacttaa
     3361 tggcaaaaat ggatagattt agcgagtata gaattatgtg gattttagta ttattcgact
     3421 taccaactga aacaaaaaaa gaacgtgctg ctgcttcaaa gtttcgcaag caactaattg
     3481 aagatggttt taatatgttt cagttttcta tttatactcg tcattgtgca agtcgcgaaa
     3541 atgctgaagt tcatatcaaa cgcgtaagaa gatttatacc agagcaaggc acagtaagca
     3601 tactttgtat tactgacaaa caatttggaa atatggaaat tttctatggc aaaaaagaga
     3661 aagaacctcc atcgggctgg caacaattaa aactttttta gaaaagaaaa acttatatta
     3721 taaaaaacaa aatcccgatt tttggtcggg attttttatt tctaaaacag tctgtaagtt
     3781 tctgattttt agtacgatac ccatatcgta ctgtttcaga ccacaaagat acaaaatttt
     3841 gaaaccaatt cacaacactg tcctcatccg ccttaatata tagctcactg tttcagacca
     3901 caaagataca aaattttgaa accaattcac aacagtatag ccaaaaaac
//
LOCUS       DAOPNX010000131         3909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_25170_length_3909_cov_5.826414, whole genome shotgun sequence.
ACCESSION   DAOPNX010000131 DAOPNX010000000
VERSION     DAOPNX010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25170_length_3909_cov_5.826414"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PKN53_10410"
     CDS             <1..446
                     /locus_tag="PKN53_10410"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOD89402.1"
                     /translation="TLIGCLMLGNLLREAGVVERLSKMVQNELINIVTLLLGISVGAT
                     ASAEVFLTEQTIKIIVLGVVAFGFGTTGGVLIAKFMNLFSKEKINPLIGSAGISAVPM
                     AARVSQAEGQKANPSNFLLMHAMGPNVAGVIGSAIAAGLLLSFLG"
     gene            600..1010
                     /gene="mce"
                     /locus_tag="PKN53_10415"
     CDS             600..1010
                     /gene="mce"
                     /locus_tag="PKN53_10415"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOD89403.1"
                     /translation="MELTHIEHIGIAVKSIEEAKKYYEDVLGLKCYAIEEVKDQKVKT
                     AFFKIGQTKIELLESTDPEGPVGKFIEKKGEGIHHIAFATKNIEEVLKNVEAKGVRLI
                     DAVPRKGAEGLDIAFLHPKSTFGVLTELCEDKNK"
     gene            1044..2603
                     /locus_tag="PKN53_10420"
     CDS             1044..2603
                     /locus_tag="PKN53_10420"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOD89404.1"
                     /translation="MAIENNIKKLVDMRAEARMGGGAKRIESQHAKGKLTARERINLL
                     LDEGSFEELDMFVTHSITEFGLGDKQFLGDGVVTGFGTIDGRLVYVYSQDFTVLGGSL
                     SETNARKICKVMDMAMKNGAPVIGLNDSGGARIQEGVLSLAGYAEIFQRNIEASGVVP
                     QLSAILGPCAGGAVYSPALTDFILMVEDISYMFVTGPKVAKTVTGEDITVEKLGGPMV
                     HASKSGVAHFVSQNEEEAIATLRKIFSYIPQNFRENPPRIENTDPIDRLCDNLNTIIP
                     ESSKKPYNILDVITTLVDNGEFLEVHKHYAKNIVVGFARFNGQSVGVVANQPNFLAGV
                     LDIEASRKGARFVRFCDCFNIPIVTLVDVPGFLPGSTQEYGGIISNGAKLMFAYGEAT
                     VPKVTVTLRKSYGGAHDVMSCKQLKGDLNYAWPTAEIAVMGDSGAVEILNAKEINAVE
                     SEEERAKFIEQKEQEYRDTFANPYQAAKHGYIDDVIEPRNTRFRIIRALASLATKKSM
                     MPAKKHTNIPL"
     gene            2615..2965
                     /locus_tag="PKN53_10425"
     CDS             2615..2965
                     /locus_tag="PKN53_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89405.1"
                     /translation="MFTTKLFLAIQWGDVFALIGICFTIVLCIMALFVLILQLFAKVS
                     KKKTVGQPKDNEKQIIEKNEVKSSKDTKDGEIAAFALAISLYLNEVHDNESLVLTIKN
                     KENSNWNNLNYYLQ"
     gene            2962..3402
                     /locus_tag="PKN53_10430"
     CDS             2962..3402
                     /locus_tag="PKN53_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOD89406.1"
                     /translation="MKKYVLKIDDKVYNVDVDVNDDIADVSVNGNKYNVLIESIIDDN
                     NPTPVVKKKVVTTAEQPKSEPVVEAKSSDGAKEMITSPLPGNVLKVEVEPNSKVKRGD
                     ILIIIEAMKMENNILAPHDGVVEKVFVKAGDVVMLGDELVSLGK"
     gene            3414..>3909
                     /locus_tag="PKN53_10435"
     CDS             3414..>3909
                     /locus_tag="PKN53_10435"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOD89407.1"
                     /translation="MKSIKKVLLLIFVFAFSIIFSPILNAKSVNSTENNQKSTTEQTV
                     VTSENSANAVQDEGFDVKENLIKFAKDSGIYALFTTHWKALIMIVISCILLYLAIVKQ
                     YEPLLLLPIAFGMLLSNMPGGEMYHTELFADGKIHWANFFQTAGLIDYLYLGVKFGIY
                     PCLIF"
BASE COUNT         1384 a          522 c          767 g         1236 t
ORIGIN      
        1 ctacattaat tggttgtttg atgctcggaa acttattaag agaagcaggt gttgtagaaa
       61 gattaagtaa aatggttcaa aatgaattga ttaatattgt tactctttta cttggaattt
      121 ctgttggtgc aactgcaagt gcagaagtgt ttttaaccga acaaacaatt aaaataatcg
      181 tattgggagt tgttgccttt ggttttggaa ctactggagg cgttttaata gcaaaattta
      241 tgaacttatt ctctaaagag aaaataaacc cattaattgg atctgcagga atttcggcag
      301 tgcctatggc tgctcgtgtt tcgcaagccg aaggacaaaa agccaatcca agtaacttct
      361 tgttgatgca cgctatggga cctaatgtgg caggagtaat aggctctgct atagcagcag
      421 gtttattatt gagttttctt ggataaataa aaaaatgtaa atgccttgat ttagaggcat
      481 tttttttatg cttaacaaca gtgagttttt cctaaaaata tcttaaaaat gtgttttaaa
      541 atagtttgat tataaattgt ttttgctatg tttgcctaaa ttaaaaaatt tattgtttta
      601 tggaattaac acatatagag cacattggaa ttgccgtaaa atctatagaa gaggcaaaaa
      661 aatattacga agatgtttta ggcttaaaat gctatgcaat tgaagaggta aaagaccaaa
      721 aagtaaaaac agcttttttc aaaattggtc aaacaaaaat tgaactttta gaatcaaccg
      781 accctgaagg accagttggt aaatttattg agaaaaaagg agaaggaatt catcacatag
      841 catttgcaac aaaaaacatt gaagaagttt taaaaaatgt tgaagcaaaa ggagttaggt
      901 taattgatgc tgtccctcgt aaaggggctg aaggtttgga tatagcattt ttgcatccta
      961 aatcaacttt tggagttttg acagagttat gcgaagataa aaataaataa cacatatagt
     1021 ttcacattta agaaaaataa aaaatggcaa ttgaaaataa cattaaaaag ttagttgaca
     1081 tgcgtgctga agctcgcatg gggggaggag caaaaagaat tgaatctcaa catgcaaaag
     1141 gcaaacttac tgcaagagaa cgcataaatt tattgttgga cgaaggtagt tttgaagaac
     1201 tcgacatgtt tgtaacacat agtattaccg agtttggact tggcgataaa caattcttag
     1261 gagatggcgt agttacaggt tttggaacta ttgacggcag attagtttat gtttattcac
     1321 aagattttac agttttgggt ggttctttat ctgaaactaa tgctcgaaaa atctgtaaag
     1381 taatggacat ggctatgaaa aatggagcgc cagtcattgg actaaatgat agtggaggag
     1441 caagaatcca agaaggcgtt ttaagtttag caggttatgc cgaaatattc caacgcaata
     1501 tagaagcatc aggagtagta cctcaattgt cagctattct tggtccttgt gcaggtggag
     1561 cagtttattc tccagctctt accgatttta tcctaatggt tgaggatatt agctatatgt
     1621 ttgttactgg tccaaaagta gcaaaaactg ttacaggcga agatattact gttgaaaaat
     1681 taggcggtcc tatggttcat gcttctaaat ctggtgttgc tcactttgta tcacaaaacg
     1741 aagaagaagc tatagctaca ctaagaaaaa tcttctcata tattccacaa aactttagag
     1801 aaaatcctcc acgtatagaa aatactgacc caattgacag attatgtgat aatttgaata
     1861 ctattatccc agaaagttca aagaagccat ataacattct tgatgtaatt acaactcttg
     1921 ttgataatgg cgaattttta gaagttcata aacattatgc aaaaaatatt gttgtaggtt
     1981 ttgctagatt taatggacaa tctgttggtg ttgttgctaa tcaaccaaat ttcttggcag
     2041 gagtgttaga tattgaagca tcaagaaaag gagcaagatt tgttcgtttc tgcgactgct
     2101 ttaatattcc aatagttact ttagtagatg ttcctggatt tttacctgga agcacacaag
     2161 aatacggcgg aattatatct aatggtgcta aattaatgtt tgcttatggc gaagctactg
     2221 tgccaaaagt aactgtaaca cttagaaaat cgtatggtgg tgctcacgat gttatgagtt
     2281 gcaagcaatt aaaaggcgac cttaactatg cttggcccac agcagaaatc gcagtaatgg
     2341 gtgattctgg agcagtagaa attttaaatg ctaaagaaat aaatgcagta gaatcagaag
     2401 aggaaagagc aaaatttatt gaacagaaag aacaagaata tcgagatact tttgctaatc
     2461 cttatcaggc agcaaaacat ggttatatcg atgatgttat agaacctcgt aatactagat
     2521 ttagaataat tagagcactt gcttctcttg caactaagaa aagtatgatg ccagcaaaaa
     2581 aacatactaa tattccactt taatttttaa taacatgttt acaacgaaat tatttttagc
     2641 tattcaatgg ggcgatgttt ttgccttaat tggtatttgt tttacaatag tgttgtgcat
     2701 aatggcactt tttgttttaa tcttgcaatt atttgcaaaa gtaagtaaaa agaaaacagt
     2761 tggacaacct aaagataatg agaaacaaat aatagaaaag aacgaagtta agtctagtaa
     2821 ggacactaaa gatggagaaa ttgcagcatt tgctcttgca ataagtttgt acttaaacga
     2881 agttcatgat aatgaatctt tggttctaac aataaaaaat aaagaaaatt caaattggaa
     2941 taatttaaat tactatttac aatgaaaaag tatgttttaa aaatagacga caaagtttat
     3001 aatgtagatg tagatgtgaa tgatgacata gctgatgtgt cggtcaatgg taataaatat
     3061 aatgtgttga ttgaaagcat tattgatgat aataatccta caccagttgt gaaaaagaaa
     3121 gtagttacta ctgccgaaca accaaaaagc gagccagtag ttgaagctaa gagttcagat
     3181 ggtgctaaag aaatgataac atcaccattg ccaggtaatg ttcttaaagt tgaagtagaa
     3241 ccaaattcta aagtgaaacg tggcgatatt ttaataatta ttgaagctat gaagatggaa
     3301 aacaatatac ttgcacctca cgatggagtt gtagaaaaag tatttgttaa agctggtgat
     3361 gttgtaatgt tgggtgatga actcgtaagt ttgggaaaat aaattttatt actatgaaat
     3421 cgataaaaaa agttttgctt ttaatatttg tatttgcttt ttcaataatt tttagtccta
     3481 tactaaatgc aaagtctgtt aattctacag aaaataatca aaaaagcaca actgagcaaa
     3541 cagtagtaac aagtgaaaat tctgcaaatg cagttcaaga cgaaggtttt gatgttaaag
     3601 aaaatttgat aaagtttgca aaagattcgg gtatttatgc cttattcaca acacattgga
     3661 aagctctgat aatgattgtg attagctgta ttcttttgta tttagcaatt gtaaagcaat
     3721 atgaacctct gcttttgttg cctattgcat ttgggatgct gttatctaat atgccaggtg
     3781 gcgagatgta tcataccgaa ttgtttgctg acggcaaaat tcattgggct aactttttcc
     3841 aaacagctgg tttgattgac tatttatatc tcggtgtaaa atttggaatt tatccatgtt
     3901 taattttta
//
LOCUS       DAOPNX010000132         3781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_26333_length_3781_cov_5.442029, whole genome shotgun sequence.
ACCESSION   DAOPNX010000132 DAOPNX010000000
VERSION     DAOPNX010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26333_length_3781_cov_5.442029"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PKN53_10440"
     CDS             complement(<1..1120)
                     /locus_tag="PKN53_10440"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOD89408.1"
                     /translation="MKLIFLIAGIFLFSLGFAQHPGNIPSDGNLSGIVLEKETNQAVE
                     YANVVLYSKRDSSIVTGGITDKNGYFNIDNIRYGKYYVEIHFIGYGKHTIPDLSISPN
                     RKQVNLGKIYLTVDAHILNEVSVEAQVNQVEYKLDKKVVNVNQDILSAGATAVEALEN
                     VPSIETDIEGNVSLRGSGSFMVLIDGRPSPLEGSEALQQIPASSIENIEIITNPSAKY
                     DADGVSGIINVILKKDKRAGWNGQISANYGSFNTHGGDALINIRTNKLNFFVGGEYSK
                     RIFRSEGTVDQISFIDDTKDFYLNTLSENSRIRGSGSARTGLDYYITERDIVTISGKY
                     GYGSWGSTAISKTENGFGKNDVIFNPEYYVSDGESQSVY"
     gene            complement(1231..1473)
                     /gene="atpC"
                     /locus_tag="PKN53_10445"
     CDS             complement(1231..1473)
                     /gene="atpC"
                     /locus_tag="PKN53_10445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOD89409.1"
                     /translation="MIVEIITPEEKLFAGETSMVRVPGTLGSFQILNNHAPIVSTLEK
                     GIITIRQKENNEEINFNIVSGLIECKKNKIIILVEV"
     gene            complement(1476..2984)
                     /gene="atpD"
                     /locus_tag="PKN53_10450"
     CDS             complement(1476..2984)
                     /gene="atpD"
                     /locus_tag="PKN53_10450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOD89410.1"
                     /translation="MSQTVGKIVQVIGPVIDVSFEKSGGELPKIHDALEIKRDDGSTL
                     VVECQQHIGEFTVRTISMASTDGLYRGMDVYDTKNPIQIPIGDQVKGRLMNVVGENID
                     GLSSLSKENAYAIHRDPPKFEEFSTEVEVLYTGIKVIDLIEPYSKGGKIGLFGGAGVG
                     KTVLIQELINNIAVGYDGLSVFAGVGERTREGNDLLREMIEANIVRYGDKFKESMEKG
                     SWDLELVENDELAESKLSMVFGQMNEPPGARARVALSGLTIAESFRDGGSKTSGRDIL
                     FFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGEMQERITSTKTGSITSVQAV
                     YVPADDLTDPAPATTFSHLDATTVLSRKIAGLGIYPAVDPLDSSSRILAPEIVGKEHY
                     ETAQRVINILQRYTELQDIIAILGMDELSDEDKLVVHRARRVQRFLSQPFHVAEAFTG
                     LKGAFVKIEDTIKGFNMILDGEVDQYPEAAFNLVGTIEQAIAKGEKMLAETK"
     gene            complement(3157..>3781)
                     /locus_tag="PKN53_10455"
     CDS             complement(3157..>3781)
                     /locus_tag="PKN53_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOD89411.1"
                     /translation="KYIAKAKYLKESLELFSDTALKICEGQQLDMDYESMTDLNVDDY
                     LKMINLKTAVFLAASLKLGAITANTNQENKNNIYEFGRNLGIAFQLQDDFLDVYGDTQ
                     NFKKNLGGDIVSNKKTFLLLKALELAKGNELKELKHWISLKEFDEQEKINAVKNIYDS
                     LKIAKHSEEKNNFYMNQASQYFEKIEADKNRKDILMNYTKSLEKRKF"
BASE COUNT         1185 a          704 c          498 g         1394 t
ORIGIN      
        1 gatatacaga ttgcgattca ccatcagaca cataatattc aggattaaaa attacatcat
       61 ttttcccaaa gccattttct gtcttactga tagctgttga accccaagag ccataaccat
      121 attttcctga aattgtaaca atatctcttt ctgttatata ataatctaat cctgttctag
      181 cgctacccga acctctaatt ctagagtttt cgctcaatgt attcaaataa aaatctttag
      241 tatcatcaat aaaacttatt tgatcaacag ttccttcgct tctaaatatt cgctttgaat
      301 attcaccgcc tacaaaaaag tttaatttat tagttctaat atttatcaaa gcatctcctc
      361 catgtgtatt aaagctacca taatttgcgg atatttgccc attccaacct gcacgtttat
      421 ctttttttag aataacattt attattcctg aaactccatc ggcatcatat tttgcagaag
      481 ggtttgttat aatttcaata ttttctattg agcttgctgg aatttgttgt aaagcctcac
      541 ttccttctaa cggacttggt cgtccatcaa ttaaaaccat aaaacttcca gaaccacgta
      601 aagaaacatt tccttcaata tctgtttcaa tagaaggtac attttctaaa gcctctacag
      661 cagttgcacc agccgaaagt atatcttggt taacgttaac aactttttta tctaatttat
      721 attccacttg atttacttgt gcttcaacac taacctcatt taaaatatgt gcgtcaactg
      781 ttaggtaaat tttacctaaa ttaacttgtt ttctatttgg actaatgctt aaatctggaa
      841 ttgtatgttt tccgtaacca ataaaatgaa tttcaacata atattttcca tatctaatat
      901 tgtcgatatt aaaatatcca tttttatcag taattccacc ggttactata ctagaatctc
      961 gtttgctgta taaaaccaca tttgcatatt caacagcttg gttagtctct ttttcaagta
     1021 ctattccact taaatttcca tcagaaggta tatttcctgg gtgttgtgca aaaccaagac
     1081 taaaaagaaa aattcccgct attaaaaaaa tcaacttcat ataatatatt attaaattag
     1141 agttagacag ataaaaaaga aaaaggttta ataaatttta aacctttttc ccaaattatt
     1201 aaacaataaa taaaacgtaa aagtttaaat ttaaacttct accaaaatta taattttatt
     1261 ttttttacat tctattaatc cactgacaat attaaaatta atttcttcat tattttcttt
     1321 ttgtctaatg gtaataattc ctttttctag tgttgaaaca attggtgcgt ggttatttaa
     1381 aatctgaaaa gatcctaatg taccgggcac tctaaccatt gatgtttctc cggcaaaaag
     1441 tttttcttca ggtgttataa tttctactat cataattatt ttgtttcagc gagcattttt
     1501 tcgcctttag cgatagcttg ctctattgta ccaactagat taaacgcagc ttctggatat
     1561 tgatccactt caccatcaag tatcatatta aatcctttta ttgtgtcttc gattttcacg
     1621 aatgcacctt ttaagcctgt aaatgcttcg gctacgtgga atggttgaga caagaatctt
     1681 tgtactcttc ttgcacggtg aacaactaat ttatcttcgt cagaaagttc gtccataccg
     1741 aggattgcga taatatcttg aagctctgta tatctttgaa gaatgttaat aactctttga
     1801 gcagtttcgt agtgttcttt tccaacgatt tctggagcca aaattcttga agaagaatct
     1861 agagggtcaa cagcaggata aatacctagt ccggcaattt tacggcttaa tactgtagta
     1921 gcatcaaggt gcgagaatgt tgttgctgga gctgggtcgg taaggtcgtc agcaggaaca
     1981 taaactgctt gaacagatgt aatagaacct gtttttgttg aagtaatacg ttcttgcatc
     2041 tctcccattt ctgttgctag cgttggttgg tatccaactg ctgatggcat acgtcctaaa
     2101 agagcagaaa cttcagaacc agcttgggta aatctaaaga tattatccac gaagaataaa
     2161 atatctctac cactagtttt tgagccacca tctctaaaac tttcagctat tgttagccct
     2221 gataaagcca cacgtgcacg cgctcctgga ggttcgttca tttgtccaaa aaccattgaa
     2281 agcttagatt ctgctaattc atcattttca acaagttcta aatcccaaga acctttttcc
     2341 attgattctt taaatttatc accatatctt acaatgtttg cttctatcat ttcgcgaagt
     2401 aagtcgtttc cttcacgggt tctttctcct actccagcaa aaactgataa tccgtcataa
     2461 cctacagcaa tattgttgat taattcttga attaaaactg tttttccaac acctgcaccg
     2521 ccaaacaatc caattttacc acctttagaa taaggttcaa ttaagtcgat aactttaatt
     2581 ccagtataaa gaacttcaac ttctgtacta aattcttcga attttggtgg atcgcgatga
     2641 atagcataag cattttcttt acttagtgaa gataatccat cgatattttc accaacaaca
     2701 ttcatcaaac gaccttttac ttggtcgcca attggtattt ggataggatt tttagtgtca
     2761 taaacatcca ttcctctata aagaccatct gtagaagcca tagagatagt acgaactgta
     2821 aattctccaa tatgttgctg acactcaaca actaaagtag aaccatcgtc acgttttatt
     2881 tctaaagcat catgaatttt tggaagttct ccgccagatt tctcaaagct tacatcaatt
     2941 acaggaccaa taacctgaac aattttacct actgtttgac tcattgtata atatgtatta
     3001 aaattattgc aaacaaaatt ataaattata tttacaataa aaaataatag atgtttttat
     3061 ttactcacat ttattaactt ttatgcaaaa cgcaattagt ttgctacatt agaaggcttg
     3121 cttactacta actgcaaaaa tcgccaacat ttccctttaa aattttcttt tttctaacga
     3181 ctttgtataa ttcattagta tgtcttttcg atttttgtca gcttcaattt tttcaaagta
     3241 ttgacttgcc tgattcatgt agaaattgtt tttttcttca gaatgtttag caattttgag
     3301 tgaatcgtaa atatttttta ctgcatttat tttttcttgt tcatcaaatt cttttaaact
     3361 tatccaatgt tttagctctt ttaactcatt tccttttgca agttctaaag ctttaagcag
     3421 taaaaatgtt tttttatttg aaactatatc tccaccaaga tttttcttaa agttttgggt
     3481 atcaccataa acatctaaga aatcatcttg taattgaaat gctattccga gatttctacc
     3541 aaattcgtaa atattatttt tattttcttg attagtattt gccgtgattg ctcctaattt
     3601 taaagaagct gccaaaaaga ctgctgtttt tagattaatc atttttaaat aatcatctac
     3661 attcaaatca gtcatacttt cataatccat gtctaattgt tgaccttcgc aaatttttaa
     3721 tgcagtatca gaaaacaatt ctaaactttc ttttaaatat ttagctttag caatatattt
     3781 g
//
LOCUS       DAOPNX010000133         3736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_26740_length_3736_cov_4.406140, whole genome shotgun sequence.
ACCESSION   DAOPNX010000133 DAOPNX010000000
VERSION     DAOPNX010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26740_length_3736_cov_4.406140"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1727)
                     /locus_tag="PKN53_10460"
     CDS             complement(<1..1727)
                     /locus_tag="PKN53_10460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HOD89412.1"
                     /translation="MKKKVQQISLEGKFDVKDILKSLDLIKNALKGQDIKDGFAQKFR
                     REMERVERSAETIEKGISKGFKSSEDTARFNAEIKSLVNNFNNLTESIKRTKVNLEDI
                     EVSDIGKRAGLDGLTRQIEEANKEIDKLRNKDVLKSRIIDTGGIKLNVSKSMASELAR
                     SLFDPTVYAVKLKEMIKTIPVSKEFSDLGQRLNIDIDTPEGLLKAKQEFEKIKKLTEE
                     IAQIQNSGMAGWKNKAGKLIQQYERENTGALPSNILSGGDLTTRVGNLQSFVGSFTNM
                     EGQLEAFQRIVNLPNTITEEIQQQEVAINNNRQAFQDMADTMTGATHSQIVSSVDIAT
                     GSFDSLTRELNSQTKAADEHIKKTERMNAVFNDLGNRLKTFASVFTLWTFGRKQIEET
                     WETIKKLDEGFASIAMVTSRSLESMWQQYSEYAEMSKKYGASTLSVIESSALFYQQGL
                     DTNEVMEVTAETMKLATLSGLGFQQTTDLMTGAIRGFKLEMAEAGRVTDVYSELAAKA
                     AADVREISYAMSKTASIAKESGMEFETTSAFLTQMIETTRESPENLGSAMKAVIARFQ
                     EVSKDPSITV"
     gene            complement(1738..3312)
                     /locus_tag="PKN53_10465"
     CDS             complement(1738..3312)
                     /locus_tag="PKN53_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89413.1"
                     /translation="MSKELIIANSWDKNCGWREYRRFLFDKYPDGSYGVKKKLALPET
                     YVHRQFERHFEENDISYQSKLSNKFSKSGFMAIVQGQKQKEQRKIIPLAKKVAKILKT
                     EEPQTLEESCRFLSNHLKVGSTIDKEEETLFEQTLLKGIEDIFNQVFLNENFSFNSIS
                     LIAMLNILKTNPEDLELIIQGLTKVLSQIQSQVGSANAEIEQTLLKTYIKRLSGAITY
                     LAQTNKGQYNAKSKIEKEIKNSFNEIGIIFKERKLFKDKYLDSGLVNLFDFKASKEGL
                     SSKEKGDAYEQQLGDNLADNLSYELTKALEKGTSSSSLKIKGKTIGTKRIRGKQQKID
                     FVWNYSFGVGKNKVQKQINFSAKNYTTTNLEFHKGGFSALTNKDWLGKQSGSANFAKY
                     LQSPAFKYWYSNTVFQMISSNESFLGMDAFLDDLKRMSALFLGGRSKQDEVDFLFVQD
                     KIVPLSLILERYQNAIVINNRNNFSNNLLKANRTQQLNDRLFPEGINIGKTIYKKANF
                     SFRTAQAAMNKILSLV"
     gene            complement(3305..>3736)
                     /locus_tag="PKN53_10470"
     CDS             complement(3305..>3736)
                     /locus_tag="PKN53_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89414.1"
                     /translation="TKKIEKNFYQNVFCYDLDMNRLSPISQDGKVITFETANLDVIVY
                     YSFEKDVKKTLYTLSKGDTMFFKILGEIVYIDEQTKLKSSCVIEFPKAIIRPSLSYQL
                     GADNNFTSYDFEFTALADENDNKKYSNKLTFLVGDTIEPYV"
BASE COUNT         1093 a          665 c          467 g         1511 t
ORIGIN      
        1 acagttatag aagggtcttt tgaaacttct tgaaaacgtg ctataacggc tttcatagca
       61 gaacctaagt tttcaggcga ctctctagtt gtttctatca tttgtgttaa gaaagcagac
      121 gttgtttcaa attccatgcc actttcttta gcaattgatg ctgtttttga catagcataa
      181 gaaatttctc ttacgtcagc agctgctttt gcggcaagtt ctgaataaac gtctgttact
      241 cttcctgcct ccgccatttc tagcttaaat ccacgaattg cccctgtcat caagtcagtt
      301 gtttgttgga aacctaaacc agataatgtt gccaatttca tagtctcggc tgttacttcc
      361 ataacctcat tcgtgtctaa gccttgctga tagaataaag cagaactttc tattacagat
      421 agagtggatg ctccatattt tttagacatt tccgcatatt ctgaatactg ttgccacata
      481 ctttcaagac ttcttgaagt aaccattgct atacttgcaa aaccttcatc aagtttttta
      541 atagtttccc atgtttcttc aatttgtttt ctaccaaaag tccaaagtgt aaaaacagaa
      601 gcaaatgttt ttagcctgtt acctaaatca ttaaataccg cattcattct ttcagtcttt
      661 ttaatatgtt catccgccgc tttggtctgc gaatttaatt ctctagttaa agagtcaaaa
      721 gaaccagtag caatatcaac cgaactaact atttgactat gagtcgcccc agtcattgta
      781 tcagccatat cttggaaagc ttgtctattg ttatttatcg caacttcttg ttgctgaatt
      841 tcttctgtaa tggtatttgg caaattaact atcctttgaa aagcttctaa ttgtccttcc
      901 atgtttgtaa aagaaccaac gaaactttgt aaattaccaa ctcttgttgt taaatctcca
      961 ccacttaata tattagatgg taaagcacct gtattttccc tttcgtattg ctgtattaat
     1021 ttaccagctt tatttttcca gccagccatt ccagaatttt gaatttgagc aatttcttca
     1081 gttagttttt ttattttttc aaattcctgt ttagctttta atagcccttc tggggtatca
     1141 atatctatat tgagtctttg ccccaaatca ctaaattcct tagaaactgg aatagttttt
     1201 atcatttcct ttaattttac tgcataaact gtagggtcaa ataaagacct tgccaattct
     1261 gatgccattg atttagatac atttaatttt atcccacctg tatcaataat tctacttttt
     1321 aatacatctt tatttctaag tttatctatt tctttattag cttcttctat ttgtcttgtt
     1381 agtccgtcta aacctgctct tttgccaata tcagaaactt caatatcttc taaattaact
     1441 tttgttcttt ttatactttc agttaaatta ttaaaattat ttactaagct ttttatttcc
     1501 gcattaaatc ttgctgtatc ttctgaactt ttgaagcctt ttgagatacc tttttcaatg
     1561 gtttcagcac ttctttctac acgttccatt tcacgtctaa atttttgagc aaaaccatct
     1621 tttatatctt gtccttttag ggcgtttttt ataaggtcaa gactttttag tatgtctttg
     1681 acatcaaatt ttccttctaa acttatctgt tgtacttttt ttttcatatt tacctcccta
     1741 aactagactt aagattttat tcatcgccgc ctgagcggtt ctaaaagaaa agttagcctt
     1801 tttataaatt gttttaccaa tattgatacc ttctgggaat agtctatcat ttaactgttg
     1861 tgttcgattt gcctttaaaa gattattgga aaagttattt ctattattta ttacaatggc
     1921 attttgatat ctttctaata ttaatgataa aggaactatt ttatcttgta caaatagaaa
     1981 gtctacttca tcctgttttg aacgtccacc taaaaataat gccgacattc tttttaaatc
     2041 atctaaaaag gcgtccattc ctaagaaact ttcattagat gaaatcattt gaaaaacggt
     2101 atttgaatac caatatttaa atgcaggtga ttgtagatac ttagcaaaat ttgccgaacc
     2161 actttgtttt cctaaccagt ctttattggt tagtgcggaa aatccacctt tatgaaattc
     2221 taagtttgta gttgtataat tttttgccga aaagtttatt tgcttttgca ctttgttttt
     2281 tcctacgcca aaagaatagt tccaaacaaa gtcaatcttt tgttgcttac cccttattct
     2341 ttttgttcca atagttttac cttttatctt taaactacta cttgaagttc ctttctcaag
     2401 tgcttttgtt aattcataag ataaattatc tgctaaatta tcaccaagtt gttgttcgta
     2461 agcatcacct ttttcttttg atgataagcc ctccttagaa gctttgaagt caaataagtt
     2521 aaccaatcca ctatctaaat atttatcttt aaatagttta cgttctttaa aaattattcc
     2581 tatttcatta aaagaatttt ttatttcctt ttcaattttt gatttagcat tatattgacc
     2641 tttgttagtc tgtgctaagt aagttatagc acctgataaa cgttttatat atgtttttaa
     2701 taatgtttgt tcaatttctg cgtttgcaga acctacttga ctttgtattt gagataaaac
     2761 ttttgttaat ccttgtataa taagttctaa atcctcaggg ttcgttttta aaatatttaa
     2821 catagctatt aaagatatgc tattaaaaga aaaattctca tttaaaaaaa cttgattaaa
     2881 tatatcttct attcctttta aaagtgtttg ttcaaagaga gtttcttcct ctttatctat
     2941 tgtacttcca acttttaaat gatttgataa aaaacggcaa gactcttcaa gtgtttgtgg
     3001 ctcttctgtc tttaatattt tagcaacttt ttttgccaat ggaattattt ttctttgctc
     3061 tttttgcttt tgcccctgta ctattgccat aaatccagat tttgagaatt tatttgataa
     3121 tttgctttga tatgaaatat cattctcttc aaaatgcctt tcaaattgtc tatgtacata
     3181 agtttcaggt aaagccagtt ttttctttac tccataacta ccatcagggt atttatcaaa
     3241 tagaaatctt cggtattctc tccagccaca atttttatcc caactatttg ctattattaa
     3301 ctctttagac ataaggttca atagtatcac ctaccaaaaa agttaattta ttagaatatt
     3361 ttttattatc attttcatca gccaatgcag taaactcaaa atcataactt gtaaaattat
     3421 tatctgcacc aagttgataa ctcaaactag gacgaattat tgcttttgga aattcaataa
     3481 cacaagatga tttaagtttg gtttgttcgt ctatataaac aatttccccc aaaattttaa
     3541 aaaacatagt atcacctttt gatagcgtat aaagagtttt cttaacatct ttttcaaaag
     3601 aataataaac tattacatct aaatttgctg tttcaaatgt tataactttt ccatcttgac
     3661 taattggtga taatctattc atatctaaat catagcaaaa aacattttga taaaaattct
     3721 tttctatttt ttttgt
//
LOCUS       DAOPNX010000134         3586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_28191_length_3586_cov_6.186916, whole genome shotgun sequence.
ACCESSION   DAOPNX010000134 DAOPNX010000000
VERSION     DAOPNX010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28191_length_3586_cov_6.186916"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..2116
                     /locus_tag="PKN53_10475"
     CDS             116..2116
                     /locus_tag="PKN53_10475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD89415.1"
                     /translation="MIKMFTQKFTILSLVFVLLGFTSFAQKSKDSKGNLDAGFVASVY
                     TITAGECIDFTDMSTGGPTAWLWSFPGAQTTTSTAKNPTGICYHFPGTYDVMLEVQNS
                     TQVNTEIVEACIEVLPNTETPIADFVADYTTIPAGGVVQFTDISQNGPFESYAWVFEG
                     GIPSSSTFAEPTPVGYPTVGKYKVSLRVEDADGDQDSEVKEKYINVIPAATEPPIADF
                     MADRIFIAPGDFINFTDRSKGSPYIWKWFFEGAAQTSSALQNPNAILYLAPGTYDVEL
                     IVESNMGIDTIRKVDYIIVSETDPCVAIPEAKFKASTRLVKSGTKVYFEDKSLNNPTT
                     WNWYFQGGYPTYAATSNITRGIEYNVSGFFDVSLSVNNACGSHSEHKDDYILVFSGPV
                     NVYCDTISNIEPYEGICSPGVPGASWGEIGGHNGQKIRIYADKFEQHAFERVDALIVP
                     VVKSQAGKYDSKVTFYIWDGSTVYPEIVLAQKDYFIRDIPENFNYVIEFGQAIEVDGP
                     FFVGYKINYVDVDNDGISDDRFSVSIANNRDAYSPNTLYVESNSQWATATAKFGYRTS
                     SAIRPVTCIVDVNEFEIDNNIDIYPNPATDIVYISTGKVNFGTDLDVQVIDITGRVVK
                     VLNSVVNTQEIPVNISDLPQGLYVFSLNFDGNIVNKRVMLSK"
     gene            2120..2608
                     /gene="rnhA"
                     /locus_tag="PKN53_10480"
     CDS             2120..2608
                     /gene="rnhA"
                     /locus_tag="PKN53_10480"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOD89416.1"
                     /translation="MDKPNITIYTDGACSGNPGPGGYGIVLISGQHRKEMSQGFRLTT
                     NNRMELLAVIVALEQLKKENSNVTLYTDSKYVSEAINEKWIEDWVRRKFKKVKNPDLW
                     KRFLDVYKKHNVEVVWVKGHSETVENNRCDFLATQAIKSTNLLIDENYENPVETQGKL
                     LL"
     gene            2619..3257
                     /locus_tag="PKN53_10485"
     CDS             2619..3257
                     /locus_tag="PKN53_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89417.1"
                     /translation="MKKQCLIAIFTLFLINIVQGQQIDVSALYSSSSAKQFKNNWAYD
                     LKYNHFIKKNRIGLSFRHYFYNTKYDDIYLSTEDGVSKYIEIYEPQNRRIALNLIYSY
                     TLVENEKSNLYIGCNIGINYFHLQGEKTRIENGFINGGNFKYNYRINSRLGLGFLIEY
                     EISKIFSERISTSIMINPELTAYEKFGTLGGYDPWLIDWLNFSIGLKYRFTD"
     gene            3397..>3586
                     /locus_tag="PKN53_10490"
     CDS             3397..>3586
                     /locus_tag="PKN53_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89418.1"
                     /translation="MKTTLKILLALSIASTILTMSSCKKTYDEGPNFSLRSKMNRITG
                     KWRLVSMEGVERLNPEVEQ"
BASE COUNT         1320 a          485 c          627 g         1154 t
ORIGIN      
        1 acactatctt atgctaatgt ttttcacaaa gtgtaaaatt aatttaaaaa tttcacgttt
       61 attttattta taattgtatc tttgtaaatt agcacaaatt aaaaattgta acattatgat
      121 aaaaatgttt actcaaaaat ttacaatcct aagtcttgtt tttgttttat taggatttac
      181 aagctttgca caaaaatcaa aagattcaaa aggcaattta gatgctggtt ttgtggcttc
      241 ggtttataca attacagctg gcgaatgtat tgattttaca gatatgagta caggtggacc
      301 aacagcttgg ctatggagtt ttcctggagc acaaacaacc acttctacag caaaaaatcc
      361 aacaggaatt tgttaccatt tccctggaac ttatgatgta atgttggaag ttcaaaatag
      421 cactcaagta aatactgaaa ttgttgaagc atgtattgaa gtgttgccaa atacagaaac
      481 accaattgcc gattttgttg cagattacac aacaattcct gcaggtggag ttgtgcaatt
      541 tacagatatt tctcaaaatg gaccattcga atcttacgca tgggtttttg agggtggaat
      601 tccatcaagt tcaacttttg cagaaccaac tccagtaggt tacccaacag taggaaaata
      661 taaagttagt ttgagagtcg aagatgctga tggagaccaa gattctgaag taaaagaaaa
      721 atatataaat gttattccag cagcaacaga accaccaatt gcagacttta tggcagatag
      781 gatatttata gctccgggcg attttattaa ttttacagat agaagcaagg gcagccctta
      841 tatttggaaa tggttcttcg aaggagcagc acaaacatca tcagcgttgc aaaatccaaa
      901 tgcaatttta taccttgcac caggaactta tgatgtagag ctaattgtag aaagcaatat
      961 gggtatagat accatacgaa aagtagatta tattattgtc tccgaaacag acccatgtgt
     1021 ggcaataccc gaagctaaat ttaaagcatc tactcgatta gtaaaatctg gaacaaaagt
     1081 atatttcgaa gataaatcgt taaataatcc tacaacttgg aattggtatt ttcaaggagg
     1141 ttatccaact tatgcagcaa catcaaatat tacaagagga attgagtata atgtttcagg
     1201 cttttttgat gtgtcattat ctgtaaataa tgcttgcggt tcacattctg aacataaaga
     1261 tgattatatt ttagtctttt caggaccagt taatgtatat tgtgatacaa tttcaaatat
     1321 tgaaccttat gaaggcattt gtagtccagg agttccagga gcatcatggg gagaaattgg
     1381 tggacataat ggacaaaaaa ttagaattta tgcagataaa tttgaacaac atgcttttga
     1441 aagagttgat gctttaatag ttcctgtagt aaaatcgcaa gcaggaaaat atgactcaaa
     1501 agtaacattc tatatttggg acggaagtac agtatatccc gaaatagtgc tcgcacagaa
     1561 agattatttt attagagata tccccgaaaa ttttaattat gtaattgagt tcggacaagc
     1621 aatagaggtt gatggacctt ttttcgtagg atataaaata aattatgtag atgtagataa
     1681 tgacggaata agcgatgata gattttcggt aagtattgca aataacagag atgcttattc
     1741 tccaaacacc ttgtatgtag aatctaactc acaatgggca acagcaacag ccaaatttgg
     1801 atatagaaca tcatcggcta ttcgtcctgt tacttgtata gttgatgtta acgagttcga
     1861 aattgataat aatatagata tttatccaaa tccagctaca gatatagttt acatatctac
     1921 aggaaaagtg aattttggaa cagacttaga tgtacaagtg attgatatta ctggtagagt
     1981 agtgaaagta ttaaattcag tagtaaatac acaagaaatt cctgtaaata tatctgactt
     2041 gccacaagga ttatatgtgt tctctctaaa ttttgatggt aatattgtca acaaaagagt
     2101 tatgttgagt aaataattta tggataaacc aaatattact atttacaccg atggtgcttg
     2161 ttcgggtaat ccaggaccag gaggatatgg tatagtttta atatctggac aacatcgcaa
     2221 agaaatgtca caaggtttta ggcttaccac aaataataga atggaattgc tggcagtaat
     2281 tgtcgcttta gaacaactga aaaaagaaaa ttcaaatgtt acactttata cagattcaaa
     2341 atacgtttcc gaagctataa atgaaaaatg gattgaagat tgggtaagaa gaaaatttaa
     2401 aaaagttaaa aatcccgatt tgtggaaacg atttttagat gtttataaaa aacataatgt
     2461 tgaagttgtt tgggttaaag gacattcaga aactgtggaa aataatagat gtgatttttt
     2521 agcaactcaa gccataaaat ctactaattt attaattgac gaaaattatg aaaatcctgt
     2581 ggaaacacaa ggaaaactat tgctttaaaa ctttataaat gaaaaaacaa tgtttaattg
     2641 caatttttac tctattttta ataaatattg ttcagggaca acaaatagat gtttcagcct
     2701 tatattctag tagttcggca aaacagttta aaaacaactg ggcttatgat ttaaagtata
     2761 atcattttat taaaaaaaat agaataggct tatcgtttag gcattatttt tacaatacta
     2821 aatatgatga tatttactta tcaacagaag atggggtttc aaaatatatt gaaatatatg
     2881 agcctcaaaa tagaagaatt gcattaaatt taatttattc atacacatta gttgaaaacg
     2941 aaaaatctaa tttgtatata ggttgtaata ttggtataaa ttattttcat cttcaaggcg
     3001 aaaaaacgcg aattgaaaat ggatttatca atggtggaaa ttttaagtat aactatcgca
     3061 taaatagtag gttaggacta ggttttttaa ttgagtatga aataagcaaa attttttctg
     3121 aaagaatttc tacatcaata atgataaatc ctgaactaac tgcttatgaa aaatttggaa
     3181 cgcttggagg gtatgatcca tggcttattg attggttgaa tttttctatc ggtttaaaat
     3241 atagatttac agattgaaaa agtcataatt acaaaaaatt gtcgaatcca cccaataaac
     3301 ttttttgcat ttttatttgt tttcccgttt tttttactct aaatttgtag taactaaatt
     3361 taaaaatacg cagatgtaaa tcctaaacat ataattatga aaaccacatt aaaaatttta
     3421 cttgcactta gcatagctag cacaatttta acaatgagtt cttgcaaaaa aacttatgac
     3481 gaaggaccaa atttttcatt gcgtagcaaa atgaaccgca taactggtaa gtggcgttta
     3541 gtttctatgg aaggagtaga gagattaaat cctgaagttg aacaat
//
LOCUS       DAOPNX010000135         3545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_28649_length_3545_cov_4.152436, whole genome shotgun sequence.
ACCESSION   DAOPNX010000135 DAOPNX010000000
VERSION     DAOPNX010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28649_length_3545_cov_4.152436"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PKN53_10495"
     CDS             complement(<1..744)
                     /locus_tag="PKN53_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89419.1"
                     /translation="MDMKRVILTAIGCPGGPSIIQMLRDDADIYIIGTDIRNDMLSKY
                     LVDEFYQVPAGKSEDFIPKMKELVVKTNAEVILPLATFELLTLSKHISEFENLGCKVC
                     VSNYESLNIANNKSLLYEYFKTKNYTPEYRILENSKNLKNLANEIGFPANKVVVKPFI
                     SHGSIGLRIIDNDVDLFEQYRHHKPNNLIIPMDLACLIFNKKSFDDIMLSEYLPGKEY
                     GVDILIHPITREIIKIIARDNGDVFHSEIS"
     gene            complement(744..1211)
                     /locus_tag="PKN53_10500"
     CDS             complement(744..1211)
                     /locus_tag="PKN53_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD89420.1"
                     /translation="MEDKINIIKLSKQLLLNNIDFFIKLGKDIIPRELQYWDKEHYLL
                     EVDGKWDYSFCCILEEKLMGFLICTKKSEKNFVHINKMAISKEYRSLKIGSKMLATLK
                     NTCLNNNIDAISLFTNKKNEGALNFYLKHGFQIEDIIKDETTERYYMIHKIKV"
     gene            complement(1211..2422)
                     /locus_tag="PKN53_10505"
     CDS             complement(1211..2422)
                     /locus_tag="PKN53_10505"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOD89421.1"
                     /translation="MIPINRPDISPLDLETYFQLKFSKFESGKYIKNLESDFANLFGE
                     DYYALFTASAKLSLYLLLNVLNLKGNIITSPLTCYSALNPIIATNNTPHYIDINKNSF
                     LLDENLVEKYISKNDKAIMAISLGGCASDLEKLRIIADKHKIILIEDCAQALGSKYNN
                     KLLGSFGHFAFFSFTKHLSLVGGGMLISKNKSVINQIREIQNQLDEIPKELLEYRFER
                     DFVESKLGIKDVNEFYTKKFLQKKQDYKENNPVDIFNAKHVMCKPNDLQAGMILKQLE
                     RLNNIVENRRKNRDLIADKLSTNKKIIFQADLGKSSFAKLYITTPYENKEVITKLVNL
                     GIDAKQLTKSHGIYFQESIDKTKYLFDDSIHKCTNYLMLHDKIVELPLSSKMNTNEIQ
                     YITSSINKLIS"
     gene            complement(2425..3057)
                     /locus_tag="PKN53_10510"
     CDS             complement(2425..3057)
                     /locus_tag="PKN53_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOD89422.1"
                     /translation="MQTKKRILILAPHTDDGEFGCGGTISKFIEEGAEVFYVAFSCAE
                     ESVPKGFPKDILETEVKKATSVLEIKPENLFIYKYPVRKLSYHRQEILENMVHLNKKI
                     KPDLVFTPSKYDVHQDHYTVTQEAMRAFKFTSLLGYELPWNNYVFETTCFVHLEEKHI
                     EKKLESLKCYRSQIGRKYSSEDYIKGLALTRGIQVGGKFAETFNVLRWIM"
     gene            complement(3096..>3545)
                     /locus_tag="PKN53_10515"
     CDS             complement(3096..>3545)
                     /locus_tag="PKN53_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89423.1"
                     /translation="SVNLKEVVQIYPKLPYEQVLDALSKANLFLLFNAHSNIGVGTKI
                     YDYIALNRKVLFCFEENTQSSVFGKNFNEEITSHFKNNLIIDLLTNTNSGIIVKNGIH
                     LHQVLDILLNEFETKGYIDSNIKNIENYSRKIQTEKLVNFINEIYLY"
BASE COUNT         1147 a          514 c          462 g         1422 t
ORIGIN      
        1 agaaatttct gaatgaaata catctccatt atctcttgca ataattttaa tgatttcacg
       61 agtaataggg tgtattagta tgtcaacacc atattctttt ccagggagat attcagaaag
      121 cataatatca tcaaaacttt ttttgttgaa aataagacaa gctaaatcca tagggataat
      181 taagttgtta ggcttgtgat gtctatattg ttcaaacaaa tctacatcat tatctattat
      241 tcttaaccct atacttccgt gacttataaa tggtttaaca acaactttat ttgcaggaaa
      301 gccaatttca ttagctaaat tttttaaatt ttttgaattc tctaatattc tatattctgg
      361 ggtatagttt ttagttttaa agtattcgta taataaagat ttgttgtttg caatatttaa
      421 ggattcataa tttgaaacgc aaactttaca gcccaaattt tcaaactctg atatatgttt
      481 tgaaagtgtt agtaattcga aagtagcaag tggcaaaata acttcagcat tagttttaac
      541 aactagttct ttcattttag gaataaaatc ttcacttttg ccagcgggta cttgataaaa
      601 ctcgtcaact aaatatttac tcaacatgtc gttacgtata tcggttccta ttatataaat
      661 atccgcatca tctctaagca tttgtattat tgaaggacca cctggacatc ctattgctgt
      721 taaaataact cgtttcatat ccattagact ttaattttat gaatcatata atatctttct
      781 gtagtttcat cttttataat atcttctatc tgaaatccat gttttagata aaagtttaat
      841 gccccttcat tttttttatt tgtgaataat gatatagcat ctatattatt atttaagcaa
      901 gtatttttta gggtagctaa catttttgaa ccaattttaa gtgaacgata ttctttgctt
      961 attgccattt tatttatgtg tacaaaattt ttttcacttt tctttgtgca aataagaaaa
     1021 cccattaatt tttcttctag tatacagcaa aaactatagt cccattttcc gtcaacttca
     1081 agaagataat gctctttgtc ccagtattgt agttctcttg gaattatgtc ctttccaagt
     1141 ttaataaaga aatcaatatt gttcagtaat aattgttttg ataatttgat tatattaatt
     1201 ttatcttcca ttatgaaatt aatttattta tagaacttgt aatatattga atttcattag
     1261 tattcatttt tgacgacaat ggcaactcaa ctattttgtc gtgtaacatc agatagtttg
     1321 tgcatttatg tatgctatca tcgaacaaat attttgtttt atcaatactt tcttgaaaat
     1381 aaattccgtg cgattttgtt aattgttttg catcaatacc taaattgacc aattttgtaa
     1441 ttacttcctt gttttcatag ggtgtggtaa tatataattt tgcaaaacta gacttgccaa
     1501 gatctgcttg aaaaattatt tttttatttg tagataattt gtctgctatt aaatctctat
     1561 tttttcttct attttcaaca atattattca atctttctaa ttgttttagg atcattcctg
     1621 cttgtaagtc atttggtttg cacataacat gttttgcatt aaaaatatct acaggattat
     1681 tttctttata gtcttgtttt ttttgtaaaa acttttttgt gtaaaattcg tttacatctt
     1741 taattcctag tttactttct acaaaatctc tttcaaaacg atattctaac aattcttttg
     1801 gaatttcgtc aagctgattt tgaatttcac ggatttgatt tataacagat ttgttttttg
     1861 agatcaacat tcctcctcca actaagctta gatgttttgt gaagctaaaa aaggcaaaat
     1921 gtccaaagct tcctaataat ttattgttat attttgaacc taatgcttgt gcacaatctt
     1981 ctattaaaat aattttatgc ttatcggcaa ttatgcgtag tttttctaaa tctgaagcgc
     2041 atcctcctag agaaatagcc ataattgctt tatcattttt tgagatatac ttttcgacaa
     2101 gattttcgtc aagtaagaaa ctgtttttat taatatcaat ataatgtggt gtgttgttag
     2161 tagcaataat tggatttaat gcagaatagc atgttaaagg agaagtaatt atatttcctt
     2221 ttaagtttaa aacatttagc aataaatata aactcagttt agcagatgct gtaaaaagag
     2281 cataatagtc ttcaccgaac agatttgcaa aatcactttc taaattttta atatatttac
     2341 cagattcaaa cttagaaaac tttaattgaa aataagtttc taaatccagt gggctaatat
     2401 cgggtcgatt tataggtatc ataattacat tatccacctt aatacattaa atgtctctgc
     2461 aaatttaccg cctacttgaa tgccacgtgt taaggcgaga cctttgatat aatcttcact
     2521 ggaatatttt ctgcctattt gggatctata acattttaaa gattctaatt ttttttcaat
     2581 atgtttttct tctaaatgta cgaagcaggt agtttcaaac acatagttat tccaaggtag
     2641 ttcgtagcct aataaggaag taaatttgaa agctcgcata gcttcttgag taactgtgta
     2701 atgatcttgg tgtacatcat attttgaagg agtgaagact aaatcaggtt ttattttttt
     2761 gtttagatgc accatgtttt ccaatatttc ttgcctatga tatgaaagtt tgcgaacagg
     2821 atatttatag ataaataaat tttcaggttt tatttctaat actgaagttg cttttttcac
     2881 ttcagtttca agaatgtctt tagggaatcc tttagggaca gattcttcgg cacaagagaa
     2941 agctacataa aaaacttctg caccttcttc tataaattta cttattgttc cgccacaacc
     3001 aaattcacca tcgtcggtat gaggagctaa aattaaaatt cgtttttttg tttgcataat
     3061 gtacatttat gtgcaaaatt aaaaaacaaa ctggtttagt acaaataaat ttcattaata
     3121 aaattaacaa gtttttcagt ttgtatttta cgcgaataat tttcaatatt tttaatatta
     3181 ctatcaatat atccttttgt ttcaaattca ttaagcaata tgtctaaaac ttgatgtaaa
     3241 tgaataccgt ttttcacaat tataccagaa tttgtatttg tcaacagatc aataattaaa
     3301 ttgtttttaa aatgtgaagt tatttcctca ttaaaatttt ttccaaaaac actagactgc
     3361 gtattttctt caaaacaaaa taacactttc ctatttaatg ctatataatc ataaatttta
     3421 gttcctactc caatatttga atgtgcatta aataataaaa ataaatttgc tttagataat
     3481 gcgtcaagca cttgttcata aggtaatttt ggataaattt ggactacttc ttttaagttt
     3541 acaga
//
LOCUS       DAOPNX010000136         3472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_29439_length_3472_cov_3.426690, whole genome shotgun sequence.
ACCESSION   DAOPNX010000136 DAOPNX010000000
VERSION     DAOPNX010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29439_length_3472_cov_3.426690"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /gene="gldL"
                     /locus_tag="PKN53_10520"
     CDS             complement(<1..336)
                     /gene="gldL"
                     /locus_tag="PKN53_10520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HOD89424.1"
                     /translation="MGLLSPKAMNFAYGMGAAVVIVGALFKIAHFELGPLTGTVMLAV
                     GLLTEAAIFALSAFETPAKEIDWSLVYPELAGGEAKVKDKKKEDPAEAQGLLSQKLDS
                     MLKEAKIDGQ"
     gene            complement(389..1786)
                     /gene="gldK"
                     /locus_tag="PKN53_10525"
     CDS             complement(389..1786)
                     /gene="gldK"
                     /locus_tag="PKN53_10525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldK"
                     /protein_id="HOD89425.1"
                     /translation="MKKFIAFASVLALLTSCGGSSDKGELVGVKGKKWHPEKPYGMAL
                     VPGGAFIMGKSDDDLANVQDAPTKTVTVRSFYMDETEITNSEYRQFVEWVKDSITRVR
                     LAILADEVGQTAGSGGKGGKTGSIGDFAFNDTDPAKMTPYDKYMYENYYSVGTDDDPY
                     AGRKLNKKVKLIKETSKYPDEYYSEVMDTMYLPVAESFNGLRTIDVRKLKFRYSYMDI
                     QAAAKAKVGKRSSFIKTEQVQIYPDTTVWIKDFAYSYNEPMHNDYFWHQAYGDYPVVG
                     VSWKQSKAFCAWRTLNKNAYIKSKKKKKDLINTFRLPTEAEWEYAARGGLESGAFPWG
                     GPYAKNDRGCFLANFKPNRGDYAADQALYTVEAKSYEPNGYNLYNMAGNVAEWTDSSY
                     DAASYEYVSSMNPNVPDTKNMRKVVRGGSWKDVSYFLQVSTRDFEYADTARSYVGFRT
                     VQDYMGTQVTGNTKK"
     gene            complement(2038..3189)
                     /locus_tag="PKN53_10530"
     CDS             complement(2038..3189)
                     /locus_tag="PKN53_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HOD89426.1"
                     /translation="MEFDFLSPVDNEILEYINGLSSQHMGSKVAFHTDKDFPDLDKIK
                     IAIIGVPENRGDNSSFEEANLDFIRKEFYGLFPGNWQSAIADLGDILPGNSIDDTFFA
                     LQKVVSRLLKKEIIPIIIGGSQDLIYPLYRAYDTLEQMVNLVSIDSKFDFGKQDDRFS
                     TNSYMSKIIVDEPNNLFNYCNIGYQTYFNSQEEIDLIEKLYFDCYRLGEITNNIALAE
                     PVLRDADLVNIDLNSVKSSDSGNFTNFIPNGFNGKEICSLARYAGISDKVSCFSVFNH
                     NNSRQESVLIAQIIWYFIEGFNFRSNEYPFGVKELYSKYIVPLEEQELVFYKSNKTER
                     WWIEIPFFSTTRNKMKKNTLLPCSHDEYLIACNQEIPERWWKAQRKNIV"
     gene            complement(3193..>3472)
                     /locus_tag="PKN53_10535"
     CDS             complement(3193..>3472)
                     /locus_tag="PKN53_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89427.1"
                     /translation="SQSDVAELIEEKLQKDIDKVIHNWSEEGILVQKARWGRSEITKG
                     KIKIELSKDIDAAALTLEQVKEMIAKKTPEKKASAKKAPAKKAPAKKK"
BASE COUNT         1077 a          661 c          456 g         1278 t
ORIGIN      
        1 ttgaccatca atttttgctt ctttaagcat gctgtctaat ttttgagaca ataatccttg
       61 tgcctcagca ggatcttctt ttttcttgtc tttaacttta gcttcaccac cagctaattc
      121 aggatatact aaagaccaat ctatttcttt agctggcgtt tcaaatgctg ataaagcaaa
      181 aatagcagct tcagttaaaa gtccaacagc caacattaca gtaccagtta atggtccaag
      241 ttcaaagtga gcaattttaa ataatgctcc aacaattaca acagcagcgc ccataccata
      301 ggcaaagttc atagctttag gtgataataa tcccataata aaaaaattta gttagtttta
      361 agtttatata attaagttgg tttaaatact atttttttgt gttccctgtt acttgagttc
      421 ccatgtaatc ttgaacagtt ctaaatccta cgtaacttct agcagtatct gcatattcga
      481 agtctctcgt acttacttgc aagaagtaag acacatcttt ccatgaacca ccacgaacta
      541 ctttacgcat gttcttagta tcaggaacat tagggttcat agaagaaaca tattcgtaag
      601 aagcagcatc gtaagacgag tctgtccatt ccgcaacgtt acctgccatg ttgtatagat
      661 tatatccgtt tggctcatat gattttgcct caacagtgta caacgcttga tctgcagcat
      721 aatcacctct atttggttta aagttagcca agaaacaacc tctgtcgttt ttagcatatg
      781 gtccacccca aggaaaagct ccagattcta aacctccacg tgcagcatat tcccattcag
      841 cttcagttgg taatctaaat gtattgatta aatctttttt cttcttttta gatttgatgt
      901 aagcattttt gttcaatgtt ctccatgcac aaaaagcttt agactgcttc caacttactc
      961 caactacagg ataatctcca taagcttggt gccaaaaata atcattatgc atcggctcat
     1021 tgtacgagta tgcaaaatcc ttaatccaaa ctgttgtatc aggataaatt tgtacttgtt
     1081 ctgttttgat aaacgagctt ctttttccaa ctttagcttt tgctgcagct tggatatcca
     1141 tatatgaata tctgaatttt aattttctaa catcaatagt tcttaaaccg ttaaacgatt
     1201 ctgctactgg aagatacatt gtatccataa cttcagaata atattcatca ggatatttac
     1261 ttgtttcctt gattaatttc acttttttgt taagttttct accagcataa ggatcatcat
     1321 cagttcctac gctataataa ttctcgtaca tgtacttatc atacggagtc atttttgcag
     1381 gatcagtatc attgaaagca aaatccccaa tacttccagt tttaccacct tttcctcctg
     1441 aaccagccgt ttgaccaact tcgtcagcta gaattgctaa tctaactcta gtaattgaat
     1501 ctttaaccca ttcaacaaat tgacggtact cactattagt aatctcagtt tcatccatgt
     1561 aaaaagaacg aactgttact gttttggttg gcgcgtcttg tacattagct aaatcatcat
     1621 ctgatttacc catgataaat gctccacctg gaaccaatgc cattccgtat ggtttttcgg
     1681 gatgccattt ttttccttta actcctacta actctccttt gtcgctggaa cctccacaac
     1741 ttgttaataa agctaaaact gatgcaaatg cgatgaactt cttcatataa attggggtta
     1801 ttagaatact caatttttta gggcgtaaac ctatttataa tttttgaaaa aaacaattat
     1861 taacttaaaa attaattaac atgacaaaac taaaattaaa tttcgaaaca aaaaaatatt
     1921 ttatcgttaa aatgcaaaaa aatatcgata aagtacattc ttttatatat tttgtaaatt
     1981 aattactatt ttaatttaat tattaaattt ttatttaaac tttttcttat ttttctatta
     2041 aacaatattc tttcgctgtg ctttccacca tctttctggt atttcttgat tgcaagcaat
     2101 taaatattca tcatgtgaac atggtaataa cgtatttttt ttcattttat tgcgtgttgt
     2161 tgaaaaaaaa ggtatttcaa tccaccatct ttcggtttta ttgcttttat agaacactaa
     2221 ttcttgctct tctaaaggaa ctatatactt agaatacagt tctttaactc cgaaaggata
     2281 ttcatttgaa cgaaaattaa atccttcaat aaaataccaa ataatttgtg caattaatac
     2341 agactcttgt cttgagttgt tatgattaaa caccgaaaag caacttactt tatcgcttat
     2401 gcctgcatat cttgccaaac tacatatttc ttttccatta aatccgttcg gtataaagtt
     2461 agtaaaattt cctgaatctg aagactttac tgaatttaaa tctatattaa ctaaatctgc
     2521 atctcttaaa acaggttctg ccaatgcaat attattagtt atttcaccta atctatagca
     2581 atcaaaatat aatttttcaa tcaaatcaat ctcttcttgc gaattaaaat aggtttgata
     2641 accgatatta caatagttga aaagattatt tggttcatcg acaattatct tcgacatata
     2701 cgaatttgtt gaaaaacggt catcttgctt tccaaaatca aactttgaat caatggaaac
     2761 taagttgacc atttgctcta aagtatcgta tgctctatat aatggataaa ttaaatcttg
     2821 acttcctcct attataatag gtataatttc tttttttaat aatcttgaaa caaccttttg
     2881 caatgcaaaa aaggtatcat caattgaatt tccgggaaga atatctccta aatctgcaat
     2941 tgcactttgc caattacccg gaaacaaccc ataaaactct tttcgaatga aatctaaatt
     3001 ggcttcttca aaagaggaat tatctcctct attttctgga acaccaatta ttgcaatttt
     3061 aattttatct aaatcgggaa aatctttatc ggtatgaaaa gccactttgc ttcccatgtg
     3121 ctgtgaagac aatccgttta tatattctaa aatttcatta tcaactggtg ataaaaaatc
     3181 aaattccata gtttatttct tttttgctgg tgctttttta gctggtgctt tttttgccga
     3241 tgcctttttc tcaggtgttt ttttggcaat catttctttt acttgttcca atgtcaatgc
     3301 tgctgcatca atatctttac tcaattcgat tttgatttta cctttcgtga tttctgatct
     3361 tccccaacgt gctttttgaa ccaaaattcc ttcttcgctc cagttatgaa ttactttatc
     3421 gatgtctttt tgtaatttct cttcaatcaa ttcagcaaca tccgattgtg aa
//
LOCUS       DAOPNX010000137         3458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_29588_length_3458_cov_6.031443, whole genome shotgun sequence.
ACCESSION   DAOPNX010000137 DAOPNX010000000
VERSION     DAOPNX010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29588_length_3458_cov_6.031443"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="PKN53_10540"
     CDS             complement(<1..824)
                     /locus_tag="PKN53_10540"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOD89428.1"
                     /translation="MTQQDIHNIIVAGEGEKTEFKSSFTDEVVISLTAFANAKGGAVY
                     IGISNDGKVKGVVLGKETVQNWLNEIKNKTIPSIVPNVDVVDFEDKKIVVFSVVEYPV
                     KPVSVKGRHYQRRVNSNHLLTAVEISDLSLQSRQISWDSYPYHNASFNDLNENKIKEF
                     INKVNQTGRFVLPESPHLALEKLGMLVKENPTNAAMILFSNKNLHYNVHIGRFKTPSL
                     IIDDKMISGNLYDVIRDAMQVIVGHLKFSLDISIKDASTQRKEIPEYPLEAIRELL"
     gene            complement(875..1996)
                     /locus_tag="PKN53_10545"
     CDS             complement(875..1996)
                     /locus_tag="PKN53_10545"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOD89429.1"
                     /translation="MNKNDNKKLTVTSPLLPPLEDFIIYLKEIWDSKHITNVGQFHKK
                     LEEELQEYLGVKYISLFTNGTLPIMVALQALRITGEVITTPYSFVATTHSLWWNGIKP
                     VFVDVDLKTGNLDHKKIEAAITPKTTAIMPVHVYGNPCNMEHIKEIADIYGLKVIYDA
                     AHAFGVKINGKSILEAGDMATLSFHATKTYNTIEGGALVCRDAETKKRIDYLKNFGFA
                     GETTVIAPGINGKMDEVRAAYGLLQLKHVNKAISQRQRVAKKYRKELRSVKGISFMED
                     ISGVRHNYSYFPIFVDEIAYGMTRDELYFKLREKNIFGRRYFYPLISEFATYRGLPSA
                     RKENLPNAHKLADSVICLPMYHELQTEDIDRVIELIKTK"
     gene            2453..>3458
                     /locus_tag="PKN53_10550"
     CDS             2453..>3458
                     /locus_tag="PKN53_10550"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOD89430.1"
                     /translation="MGTSVKDSFFKGIFWNAIGKYSHQGISFVVSIILSRILFPAEFG
                     MIAMLTIFTEIAICFIDSGLSVALIQKKECTNKDFSTVFYFNITVSLIFYLILFFTAP
                     FIAKFYELPQLTNITRLVALVFLINSFGTIQSTILKKNLNFKRLNLISIIAVAASAIV
                     AITMALNDFGVYSLVGQHISYAIVSNLLYWFLSDWRPSFTFSKKSFRELFGFGYKLLF
                     STILDKIFTNLDSLLIGKIFSANSLGFYTRAKTTRSLPINSTTGIVNSILLPIFSKID
                     NDEDLATVGLKIYRLFFYIIAPIMVGLIVTAEPLIITLFSDRWLPSAPWTQIICISGV
                     T"
BASE COUNT         1059 a          572 c          505 g         1322 t
ORIGIN      
        1 atcaataatt cacgaattgc ttcaagtgga tattctggaa tttcttttcg ttgtgtactt
       61 gcatctttta ttgaaatatc aagtgaaaat ttcaaatgtc ctacaattac ctgcattgca
      121 tctctgatta catcgtacaa attaccacta atcatcttgt catcgataat taatgacggt
      181 gttttaaaac gcccaatatg tacattataa tgcaagtttt tgttagaaaa caatatcatt
      241 gctgcatttg tagggttttc ttttacaagc attcccaatt tttcaagcgc taaatgcgga
      301 ctttcaggta aaacaaaacg tcctgtttga tttaccttat ttataaattc cttgatttta
      361 ttttcgttta aatcattgaa acttgcatta tgatacgggt acgaatccca cgaaatttgt
      421 cgcgattgca aacttagatc tgaaatttca acagcagtca gcaaatgatt tgaattaact
      481 ctgcgttgat aatgtctccc tttcacagaa acaggcttta caggatattc aaccacgcta
      541 aaaactacaa tttttttgtc ttcaaaatct acaacatcaa cattaggaac aatagaagga
      601 atagttttat tttttatttc gtttaaccaa ttttgtacag tttccttgcc taagacaaca
      661 cctttgactt tcccatcatt agatatacca atatacacag ctcctccttt tgcatttgca
      721 aaagctgtta gagaaattac aacttcatcg gtaaaagatg atttgaattc agttttctct
      781 ccttcaccag caacaattat attatgtata tcttgttgtg tcattttata attacattta
      841 gtttaggtta tctgcattat tcatagtttc atcattattt tgttttaata agttcaataa
      901 ctctatctat atcttctgtt tgtaattcat gatacatagg caagcaaatc acactatcag
      961 ctagtttatg tgcatttggc aaattttctt tcctagcgga aggcaatcct cgatatgtgg
     1021 caaattcact aatgagtgga tagaaatatc tccttccaaa tatgtttttt tcacgtagtt
     1081 taaagtaaag ttcatcacga gtcatgccat aagcaatttc atctacaaat attggaaaat
     1141 aggagtagtt gtgcctaaca cctgatatat cttccataaa tgaaattcct tttacacttc
     1201 ttaattcttt gcgatatttt ttagctactc gttgacgttg cgaaattgct ttgttgacat
     1261 gctttaattg taacaaaccg taagcagcac gcacttcgtc catttttcca tttatgccgg
     1321 gagcaataac tgttgtctcg ccagcaaatc cgaagttttt taagtaatca attcgttttt
     1381 ttgtctctgc atcgcgacaa acaagagctc caccctctat ggtgttataa gtcttggtgg
     1441 catggaaact gagagttgcc atatctccag cttctaaaat tgattttcca tttattttca
     1501 caccaaaagc atgtgcagca tcgtaaataa cttttagtcc ataaatgtct gcaatttctt
     1561 ttatatgctc catattgcaa ggattgccat aaacatgcac aggcataatg gcagtagttt
     1621 ttggtgttat tgcagcttct atctttttgt ggtctaagtt cccagttttt aagtcaacat
     1681 ctacaaacac aggttttata ccattccacc agagtgaatg tgttgttgct acaaaactgt
     1741 atggtgtggt aataacctcg ccagtaatac gcaatgcctg cagagctacc ataataggta
     1801 gtgtaccatt tgtaaaaagg cttatatatt taactcctaa atattcttgc aattcctctt
     1861 ctaatttttt atgaaattgt ccaacatttg taatatgctt gctatcccat atttctttta
     1921 aatatataat aaaatcttcg agaggaggaa gaagcggcga agtaacagta agttttttat
     1981 tgtcgttttt attcataata tcagatgaca aaatcaaatg tcaaatataa tattttttgc
     2041 aaaggtatat atatttttaa aatttaattt tttaggtata tattgtctta ttagtttatt
     2101 tatccttaca ttactatcaa gagtttgacc gtaggacact tctgttaaaa gttaaaagag
     2161 tttttagttt ttagttttga gtgtttagtg cttagtgtag agtttttagt attaagtcta
     2221 ttgctcgaat agatttaact ttacgaactt taaaaacttt attaactttt aactgtattg
     2281 gctacgccta cgattaatta catgttgatc tagtggacaa ggtgtaattg ttagtcaaaa
     2341 gtataacttt atgcgttctg tatgaagcta atctcaatag ttttttttaa ttaaacaaaa
     2401 atttagcgaa gttagagatt taatattact tttgcatttc taattaaaaa ttatgggtac
     2461 aagcgtaaaa gattcttttt ttaaaggaat attttggaat gcgataggaa aatatagcca
     2521 tcaaggaata tcttttgttg tatctattat tctttcccga atacttttcc ctgctgaatt
     2581 tggaatgata gcaatgctaa caatttttac tgaaattgca atatgcttta tagactctgg
     2641 tttaagcgtt gctcttattc aaaaaaaaga atgtaccaac aaagattttt ctacagtttt
     2701 ttattttaat attactgtga gtttaatttt ctaccttata ctttttttta cagctccttt
     2761 tattgcaaaa ttttatgaat taccacagct tacaaatata actcgtcttg ttgcacttgt
     2821 ttttttgatt aattcatttg gtactattca gtcaacaata cttaaaaaaa accttaattt
     2881 taaacgactt aatcttatat ctattattgc agtagcagca tctgcaatag tcgctattac
     2941 tatggcttta aacgattttg gtgtttattc actagtaggt caacatatat cttatgctat
     3001 tgtatctaat ttattgtatt ggtttttatc agattggcga ccaagtttta cgttttcaaa
     3061 aaaatcattt cgcgaacttt ttggttttgg ctacaaactt ttattttcca caatactcga
     3121 caaaatattt acaaaccttg acagtttgct tattggtaaa atattttcag ctaattcatt
     3181 agggttttat acaagagcaa aaacaacaag aagtttgcct ataaatagta caacagggat
     3241 tgtaaattca atacttcttc ctattttttc aaaaatagat aatgatgaag accttgcaac
     3301 tgttggatta aaaatatatc gtttgttttt ttacattatt gccccaataa tggttggctt
     3361 aattgttact gccgaacctt tgattattac cctgttttct gatagatggc taccttcagc
     3421 tccttggacg caaataattt gtatatctgg tgttactg
//
LOCUS       DAOPNX010000138         3415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_30081_length_3415_cov_6.897619, whole genome shotgun sequence.
ACCESSION   DAOPNX010000138 DAOPNX010000000
VERSION     DAOPNX010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30081_length_3415_cov_6.897619"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2466
                     /locus_tag="PKN53_10555"
     CDS             <1..2466
                     /locus_tag="PKN53_10555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOD89431.1"
                     /translation="NKYSPPLSLGYDIRVPYGFCDTAISAYKPWFTSYLWSTGETDSV
                     IHVNKPGVYSCTVTDIFGFESSDDIRVFYPQVNNFADTIICYGNSVIWDAELTGDYSY
                     EWLGSPETTQSIEIFYEGDYALIITDSLGCKYYSDTINFSFDKYELTASIGPADTSLC
                     AGNRLSLVTNSDETVSYNWNTGETTPEITITESGTYSVTCTNWRACSAEDEIAVTIMG
                     TVPNPDFSVQGHCAKNLISFNDQSTSPEGNINFWQWFVNGEVFASSQNSEITLDEPGN
                     YNIRLQIKTDLDCGDYLDETIEVFPLPEPKFFPDYYCQWAEIDFISKTTILSGNILNN
                     YWQFNDFDLFGDTVSYVFENSGINTVKLISESEQACRDSIIKEIFVKQAVKPDFLSQN
                     ACVNSESSFINITEQSSYNLPVSWNWNFGDGNFGENSDPSHVYENSGIYDVSLSVVWQ
                     NTCETSITKQIEIYDNPELELLVENSCVNSEFTVAKNIESQSGEIVEYIWNFNGNEII
                     SNTDASPTFVCDTIGEYPISLEIKSEYGCEAFAQDTIKVYPLPQVDFAMSRDWGGFPF
                     TVDFTNLSQGASQYLWSFGDSESSIEENPTHIYISEGEFFVNLTAVSEYGCSDYIEKK
                     IISVNPIMDLILYDLRTKLNGNYLEISVYIINNGTLPVSKPELVLNLGEGKIYRESLD
                     SIPVSQVIDYTLKTEIWRAEKTLPEIICLEVFANPYEGHYENNTEDNLICSANVKNLL
                     VFQPYPNPTNQSLNIEYILAKPAECLITITNSMGKQVYVNKFENQTQYNKLELDVSEF
                     AVGVYYLQVQSGGERESRKVVVY"
     gene            2553..>3415
                     /locus_tag="PKN53_10560"
     CDS             2553..>3415
                     /locus_tag="PKN53_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89432.1"
                     /translation="MKSKILVLLSLIVFAFLAEAQEVSNGVMYLKELRKPTYRKIINI
                     PEVDGYQVLKCDFHTHTVFSDGYVWPTIRAQEAWEEGLDAIALTEHIEYHPYKNYVEV
                     NHNRSHELIEDVSKRNNVILIKGTEITRKTPPGHFNAIFIGDASSYIEDNASEKDREA
                     VMKAHEQDAFIFWNHPGWQPNIEGSYEWLPFLEDLYKNNALHGIEVINGFGFHLKALD
                     WCIDKGLTVMGTSDIHNLIGHDYDKSKDYVHRSMTLVMAKDRTPESIREALKAGRTVA
                     WASKYLAGKEE"
BASE COUNT         1193 a          467 c          623 g         1132 t
ORIGIN      
        1 aacaaatact ccccccctct ctccctcggt tacgatatcc gtgttcctta tggtttttgt
       61 gatactgcaa tttcagctta taagccttgg tttacttctt atttgtggag tactggcgaa
      121 acagattctg tgattcatgt aaataaacca ggagtttatt catgtacagt aacagatatt
      181 ttcggttttg agtctagtga tgatattaga gtgttttatc cacaagtgaa taattttgca
      241 gatacaatta tatgttatgg aaattctgta atttgggacg cagaacttac aggagattat
      301 tcctatgaat ggcttggctc gccagaaact actcaatcaa tcgaaatttt ttatgagggc
      361 gattatgctt tgattattac cgatagtttg ggctgtaaat attatagcga tacaataaac
      421 tttagttttg ataaatatga attaacagct tctataggtc ctgcagacac ttcgctttgt
      481 gctggaaata gattgagttt ggtaacaaat tctgacgaaa ctgtaagcta taattggaat
      541 actggagaaa caacaccaga aattactata accgagtcgg gaacttattc tgtaacttgc
      601 acaaattggc gtgcttgctc ggctgaagat gaaattgcag taacaattat gggaactgtg
      661 ccaaatcctg atttttctgt gcaaggacat tgtgcaaaaa atctaatttc gtttaacgat
      721 caatctacaa gcccagaagg aaatataaac ttttggcagt ggtttgtaaa tggtgaagtg
      781 tttgcaagtt ctcaaaatag cgaaattact cttgacgaac ctggtaatta caatattcgc
      841 ttacaaataa aaacagattt agattgcggc gattatttag atgaaactat tgaagttttc
      901 ccacttccag aaccaaagtt tttcccagat tattattgtc aatgggcaga aatagatttt
      961 atttctaaaa caacaatttt aagtggaaat attttaaata attattggca atttaatgat
     1021 tttgatcttt ttggagacac tgtatcttat gtttttgaaa attctggtat taatactgta
     1081 aagttaataa gcgagtcaga gcaagcatgt agggattcta ttattaaaga aatttttgta
     1141 aaacaagcag taaaaccaga ttttctttcc caaaacgcct gtgttaacag cgaatcgtca
     1201 tttataaata ttacagagca aagttcttat aatttacctg tttcgtggaa ttggaatttt
     1261 ggtgatggaa attttggtga aaattcagac ccatcacatg tttacgaaaa ttctggtatt
     1321 tatgatgtga gtttatctgt tgtttggcaa aacacttgtg aaacttcaat cactaagcaa
     1381 attgaaattt atgataatcc agaattagaa ttattggttg aaaactcatg cgtaaacagt
     1441 gaatttactg tagcgaaaaa tattgaatca caatcaggtg aaattgtgga atatatatgg
     1501 aattttaatg gaaatgaaat tatttcaaat acagatgctt cgccaacttt tgtttgcgat
     1561 acgataggag aatatccaat tagtttagaa ataaaatcag aatatggttg tgaggctttt
     1621 gctcaagata ctataaaagt ttatccatta ccacaagttg attttgcaat gtcaagagat
     1681 tggggcggat ttccttttac tgttgatttt acaaatttaa gccaaggagc aagccaatat
     1741 ttatggtctt ttggagattc tgaaagttca atagaagaaa acccaacgca tatttatatt
     1801 tctgaaggag agttttttgt aaacttaaca gctgtgtcag aatatggttg ctcagattat
     1861 atagagaaaa aaataatatc agtaaaccca ataatggact tgattttata tgatttacgt
     1921 acaaaattaa atggaaatta tttagaaatt tcggtttata taatcaataa tggaacttta
     1981 ccagtaagca aacctgaact agttttaaat ttgggtgagg gaaaaattta cagagaaagt
     2041 ttagactcaa ttccagttag tcaagtaatt gattatacat tgaaaacaga aatttggcga
     2101 gcagaaaaaa ctcttccaga aattatttgt ttagaagttt ttgctaatcc ttatgaagga
     2161 cattacgaaa acaatactga agataattta atttgttctg ctaatgttaa aaacttattg
     2221 gtattccaac catatcctaa cccaacaaat caaagtttaa atattgaata tattcttgca
     2281 aaacctgcag aatgtttaat tacaattaca aacagtatgg gcaagcaagt ttatgtaaat
     2341 aagtttgaaa atcaaacaca atacaataaa cttgaacttg atgtttcaga atttgcagta
     2401 ggagtatatt atttacaagt acagtcggga ggagaaagag agagtaggaa agtagtggtg
     2461 tattaaaata tagaaaaatt tcatggaata cttttctaat atgcaactaa atatgtttaa
     2521 aatcttaaat tgtttaacca aaatatatta aaatgaaatc gaaaatatta gttttattat
     2581 cacttattgt ttttgctttt ttggctgaag ctcaggaagt tagtaatggt gtaatgtatt
     2641 taaaagaatt gcgtaaacct acttacagaa aaataataaa tattcctgaa gtggatggtt
     2701 atcaagtgtt gaaatgcgat tttcatactc atacagtttt ttctgatggt tatgtgtggc
     2761 ccactattcg tgctcaagaa gcttgggaag aaggattaga tgcaattgct cttacagaac
     2821 acattgagta tcatccatat aaaaattatg ttgaagtaaa tcataatcgt tctcatgagc
     2881 ttatcgaaga tgtttctaaa agaaataatg ttattttgat aaaaggaaca gaaattacta
     2941 gaaaaactcc tcccggacat ttcaacgcta tttttattgg agatgcttcg tcttatatcg
     3001 aagataatgc ttccgaaaaa gatagagagg ctgttatgaa agctcatgag caagatgctt
     3061 ttattttttg gaatcatcca ggttggcaac caaatatcga aggctcgtat gaatggcttc
     3121 cttttttaga agatttatat aaaaataatg ctctgcatgg aattgaagta attaatggat
     3181 ttgggtttca tctaaaagct cttgattggt gtatagataa aggtttaact gtgatgggaa
     3241 cttctgatat tcacaatctt attggacacg attatgataa aagcaaagac tatgttcatc
     3301 gttcaatgac ccttgttatg gcaaaagaca gaacgccaga atctattcga gaagcattga
     3361 aagcaggaag aacagttgct tgggcaagca aatatttagc aggaaaagaa gaaaa
//
LOCUS       DAOPNX010000139         3361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_30715_length_3361_cov_6.726558, whole genome shotgun sequence.
ACCESSION   DAOPNX010000139 DAOPNX010000000
VERSION     DAOPNX010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30715_length_3361_cov_6.726558"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="PKN53_10565"
     CDS             <1..1070
                     /locus_tag="PKN53_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89433.1"
                     /translation="ISGRKKTDSANPVYVRDRKLSKIDNFAIWVRGNFFIPDARKFWI
                     KPSVRYLKKYLEENPVDAIFTDGPPHTNTVIGMKLSQATKIPWLMDFQDPWTQVDYYE
                     MLKIGKRADKIHHKLEQEAFRTAKKTTIVSPTWKKDLEEIGAKNVDVIYWGYDEDDFK
                     TEEPKLDSDFSIIHAGQLGYDRRPTTFIKLLGDLKNENPEFGKHLKLKFAGTVDYAIK
                     EMIIEAGLEDNYIAFGNVSRPQAIELTRKAQVLFLPLNIADNAKGRIPGKLFENLRAK
                     RPILCLGPKGSDVSNIIKTTNTGGSFEYDDYEGIKKFLLEIFDKFLQNKNILNVENIE
                     QYTVENQTKILAGYLDELLKD"
     gene            complement(1067..1843)
                     /locus_tag="PKN53_10570"
     CDS             complement(1067..1843)
                     /locus_tag="PKN53_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89434.1"
                     /translation="MNGLIYNKSDAERFLILKSFYEKQDQIIDNDTNAGEVLSARIEK
                     HYNIFEKLKAYESFVREQKEKEDQEFKDLFNKAKIFVVHYFQALYMAIERGELSSQTA
                     NYYGLDFPFEIPNPKRSENLLKIAEDLFNADAARIADGGKYFSNPSLGAVRAWVEKFK
                     EVWLFRNSKLKSNKAEVENIENIRKDSDKLIYELYELLDKHLEDLDIIEKAKIFAEYG
                     LIIKTGEENTDTPINNINEKKRSLKAEVNQLTFDFVFPEN"
     gene            1954..2661
                     /gene="pyrH"
                     /locus_tag="PKN53_10575"
     CDS             1954..2661
                     /gene="pyrH"
                     /locus_tag="PKN53_10575"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOD89435.1"
                     /translation="MEYKRILLKLSGESLAGEKKQGLDEKCLLDYSEQIKAISKKGIQ
                     IGIVIGGGNIFRGLSGAAKGFDRVKGDQMGMLATVINSLALQSALESLGVKTKLFTSI
                     SIASIGEKFAKPKVMEALNSNFICIFAGGTGNPYFSTDTASALRAIEIEADVFLKGTR
                     VDGVYTDDPEKNPKATKFDSISFDEVYDKNLKVMDLTSFTLCKENNMPVIVFNMDKKG
                     NLEKLISGEKIGTLIKK"
     gene            2671..3231
                     /gene="frr"
                     /locus_tag="PKN53_10580"
     CDS             2671..3231
                     /gene="frr"
                     /locus_tag="PKN53_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOD89436.1"
                     /translation="MEDLELYIETAEEGMAAAVEHLEKELSKIRAGKASPAMLDSVRV
                     DYYGVVSPISQVANVTCPDAKTIFIQPWDKTVIPVIEKAILAANLGFNPANNGEVIRI
                     MVPPLTEERRLQLVKQTKALGENAKIAIRNQRRDAIEEFKKMKKDGLSEDKAKMAEQE
                     VHKTHEDYIKKVDVVIDNKEKEIMTV"
BASE COUNT         1237 a          448 c          571 g         1105 t
ORIGIN      
        1 agatttcggg acgtaaaaaa actgactcag caaaccctgt gtatgttagg gatagaaaat
       61 taagcaagat tgataatttt gctatttggg tgcgtggaaa ttttttcatt cccgatgcac
      121 gtaaattctg gataaaaccc tcagtaagat atttgaaaaa atatttggaa gaaaatccag
      181 tagatgcaat ttttactgac ggtccgccac atacaaacac ggttattggc atgaaattgt
      241 cgcaagcaac caaaattcca tggctaatgg attttcaaga cccttggaca caagttgatt
      301 attacgaaat gctaaaaatc ggaaaaagag ccgacaaaat tcatcataaa ttagagcaag
      361 aagcttttag aacagctaaa aaaacaacaa ttgtaagtcc aacttggaaa aaggatttgg
      421 aagaaattgg tgcaaaaaat gttgacgtaa tttattgggg atatgatgaa gacgatttta
      481 aaacagaaga accaaaatta gattcggatt ttagcattat ccatgcagga caacttggat
      541 acgacagacg accaactact tttataaaac ttttgggtga tttaaaaaat gaaaatccag
      601 aatttggaaa acatttaaaa ttaaaatttg caggcactgt cgattatgca attaaagaaa
      661 tgattataga agcaggttta gaagataatt acatagcttt tggaaatgtt tctcgtcctc
      721 aagcaattga actaacaaga aaagctcaag ttttattttt acctctaaat attgccgata
      781 atgcaaaagg cagaatacct ggaaaacttt ttgaaaattt aagagctaaa cgaccaatat
      841 tgtgtttagg tcctaagggt agcgatgtat cgaatattat taaaacaaca aatacaggag
      901 gctcttttga atatgatgat tatgagggga ttaaaaagtt tttattggaa atttttgata
      961 aatttttgca aaataaaaac attttaaatg ttgaaaacat agaacaatat actgtagaaa
     1021 atcaaactaa gattttagca ggatatttag atgaactttt gaaagattaa ttttctggaa
     1081 aaacaaaatc aaaagttagt tggtttactt ctgcttttag agaccttttc ttttcgttta
     1141 tattattaat aggtgtatcg gtattttctt caccagtttt gattatcagt ccgtattcag
     1201 cgaatatttt agctttttca ataatatcaa ggtcttctaa atgcttgtct aagagttcgt
     1261 aaagttcgta aataagtttg tcgctatctt tacgaatatt ctcaatgttt tcaacttctg
     1321 ctttatttga ttttagttta ctatttctaa atagccatac ttctttaaat ttttctaccc
     1381 aagctcttac tgcaccaagt gatggatttg agaaatattt tccaccatca gcaattcttg
     1441 cagcatctgc attaaataaa tcttctgcaa ttttaagtag attttcagat cttttagggt
     1501 ttggaatctc gaatggaaaa tctaatccgt agtagtttgc agtttgagaa ctaagttctc
     1561 cacgttcaat agccatgtat aaagcctgaa aataatgaac aacaaaaatt ttagccttat
     1621 taaaaagatc tttaaactct tggtcttctt tttctttttg ctctctaaca aaactttcgt
     1681 aagcttttag tttttcaaaa atgttatagt gtttttcaat tcttgcactt agaacttcgc
     1741 ctgcatttgt atcattatca atgatttgat cctgtttctc gtagaaagat tttaatatta
     1801 aaaacctttc agcatcactt ttattataaa tcaacccgtt catatttaac atttgataca
     1861 aatatataat cttgttttca tataatttat atggattaaa tattttattt aacattaaat
     1921 tgtttatttt tgtaaacaaa ttcattttta atgatggaat ataaaaggat attactgaaa
     1981 cttagtggcg aatcacttgc aggagaaaag aagcaaggtt tagatgagaa atgtttgtta
     2041 gattattctg aacaaataaa agctatttct aaaaaaggaa ttcaaatagg aattgtaatt
     2101 ggtggaggaa atatttttag aggtttgtct ggtgctgcca aaggttttga tagggttaaa
     2161 ggcgaccaaa tgggaatgtt ggcaactgtt ataaattctc ttgcattaca gtcggcttta
     2221 gaatctttag gtgttaaaac taaacttttt acctcaataa gcatagcttc tattggcgaa
     2281 aagttcgcaa aacctaaggt aatggaagct ttaaattcaa attttatttg tatatttgct
     2341 ggaggtacag gaaatccata cttttctaca gatactgctt ctgctttacg tgcaattgaa
     2401 attgaagcag acgtttttct aaaaggtaca cgtgttgatg gagtttatac cgatgatccg
     2461 gagaaaaatc ctaaggcaac aaaatttgat tcaataagtt ttgatgaagt gtatgacaaa
     2521 aatttaaaag taatggattt aacatcattt actttatgta aagaaaataa tatgcctgtg
     2581 attgtattta acatggataa aaaaggaaat ttagaaaaac ttataagtgg agaaaaaata
     2641 ggaactttaa taaaaaaata aactaagaat atggaagatt tagaattata tattgaaact
     2701 gcagaagaag gaatggcagc tgcggttgaa catttagaaa aagaattatc aaaaattaga
     2761 gcaggaaaag caagtcctgc aatgttggat agtgttagag ttgattatta tggagttgtt
     2821 tcgccaattt cacaagtagc aaatgttaca tgtcctgatg cgaaaacaat ttttattcaa
     2881 ccttgggata aaaccgttat cccagttatt gaaaaagcaa ttttagcagc aaatcttggt
     2941 tttaatccag ctaataacgg agaagttatt cgtattatgg tgccaccact aaccgaagaa
     3001 agacgtttac aattagtaaa acaaaccaaa gcccttggcg aaaatgcgaa aattgctatc
     3061 agaaatcaaa gacgcgatgc tattgaagag tttaaaaaaa tgaaaaaaga cggtttgtca
     3121 gaagataaag ctaaaatggc agagcaagaa gtgcataaaa ctcacgaaga ttatattaaa
     3181 aaagtagatg tcgttatcga taacaaagaa aaagaaataa tgacggttta aatcagttaa
     3241 aagttcataa agtttttaaa gttcataaag tttttaaagt tcataaagtt tttaaagttc
     3301 ataaagttcc ttaaatccgc aactaaaaat atttattgca attttagata aaattatttt
     3361 g
//
LOCUS       DAOPNX010000140         3194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_32876_length_3194_cov_4.453329, whole genome shotgun sequence.
ACCESSION   DAOPNX010000140 DAOPNX010000000
VERSION     DAOPNX010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32876_length_3194_cov_4.453329"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PKN53_10585"
     CDS             <1..391
                     /locus_tag="PKN53_10585"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0031564 - transcription antitermination
                     [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOD89437.1"
                     /translation="GEYKEVEVILYPKYIFIDMIVTDDTWFMVRNTPLVTGFLGSSGG
                     GAKPVPVSSEEMQTIFDLMGIKEEDKIEFEGQVGDQVEIISGNFKGSEGVIESIDISK
                     KIAVVLISMFGRATPAELSFSDLKVKK"
     gene            complement(468..923)
                     /locus_tag="PKN53_10590"
     CDS             complement(468..923)
                     /locus_tag="PKN53_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89438.1"
                     /translation="MKQVVKFSNLIAAILGALAIVFLAITPGMKLNVPLETEGFSGFQ
                     AIFGYSETIMGHSVEVLAPNWVGIIAFVLLAVGVIAGVIGMVIGNKMANLVSAVVLLA
                     GGILLFLVPTIANGEHETFKLSTWLIISGIIAILGGLFGCFGIFVKSKK"
     gene            1041..2795
                     /locus_tag="PKN53_10595"
     CDS             1041..2795
                     /locus_tag="PKN53_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89439.1"
                     /translation="MDKEFIYKPVDEYKEYARLHHENISQYFDELTKTANTDVGANEL
                     TVKKIRTLEKENDGIHKQQSRYQMLKVFLWILVAIAIFVFFYAIYLTASDAAVETVSL
                     VLMFIISAIVIVGGLLLIYKVIKPKYLVLEAKSQELEREISNQYKVAYQQMGSLNALF
                     KVEVIAELFEKTIPLLDFDKMFSQKTLEFLKTTFGFDDSTSEEQSTLYVQSGHLNGNP
                     FALFKNLYHVLGTKTYVGTKTISWTETIGSGKDRRTVTRTQTLTATLEKPCPYYSEQT
                     HLVFASEVAPDLTFYRKQGYTHRLTPKQVERKVRSDMEDFREDTEDNFSKSITLLAND
                     EFEVLFRALNRNNEVQFRLLFTPLAQQNMIDLLLDKTEGFGDDFAFAKKNKLNFISPD
                     HMQGFKLDITPKHFVDYDYNRCKQNWINYNMEYFRKMYFTFAPILCIPLYQQHPPHEY
                     VYRDVVESYSSFFEDEMIVNNLGYAKFSHPDSKTRNILKTKLVNINGKVEDIQVTAYG
                     YNVEKRVTYIPKTGGDGRVHQVPVHWDEYLPVVQTTDIVMDFFDDLDKIQEMMRNQDF
                     QGVLDRNMCLVKFRGKEE"
     gene            2798..>3194
                     /locus_tag="PKN53_10600"
     CDS             2798..>3194
                     /locus_tag="PKN53_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOD89440.1"
                     /translation="MANELNELKDYVNEEGRDINVIEKQIPVKVGWGSTLFEVLLWVC
                     GIIPGLIFLFMKIKAQKYFDQLQQKIQHNASQIDNYLEQRVQVLQNVVGLVERSIDLD
                     KDVMKSVAAFRGGIHPTEENRSQVASQLES"
BASE COUNT         1194 a          429 c          551 g         1020 t
ORIGIN      
        1 cggagaatat aaagaagttg aagtaattct ttatcctaaa tatattttta tcgatatgat
       61 tgttactgat gatacttggt ttatggttag aaacacaccg ctagttacag gattcttagg
      121 atccagtggt ggaggtgcta aaccagttcc tgttagttca gaagagatgc aaacaatttt
      181 tgacttaatg ggtattaagg aagaagataa aattgaattt gaaggacaag ttggcgatca
      241 agttgaaatt atctcaggta attttaaagg ttctgaaggc gttattgaaa gtatagacat
      301 atcaaagaaa attgctgtag ttttaataag tatgtttgga agagctacac cagctgaact
      361 tagtttttca gatttaaaag taaaaaaata agcccctttg aaagggctta ttttttattt
      421 taagggcatt aaaaaagctc ctatatgagg agcttaggat gtatttatta ttttttgctc
      481 ttaacaaaaa taccaaaaca accaaataat ccgcctaaaa ttgcaatgat accactgatg
      541 attaaccaag tagatagttt gaaagtttca tgttcaccat ttgcaatagt tggaactaag
      601 aacaataaaa taccgccagc aagtaaaaca acagcagaca ctaaattagc cattttgtta
      661 ccgataacca tgccaataac accagcgata acaccaactg caagcaaaac gaaagcgata
      721 ataccaaccc agttaggtgc aagaacttca acagagtgtc ccataattgt ttcagaatat
      781 ccaaaaatag cttggaagcc actgaatcct tctgtttcaa gtggaacatt taatttcatt
      841 cctggagtaa ttgctaggaa tacgatagct aaagcgccta aaatagcagc aattaaattt
      901 gaaaatttta caacttgttt catacatttc ctcctcggta agagtatatc atagtcaatg
      961 gtaaaaaaca agcaaaaaag aagcgagtat tattgtttta caaatcaata tatgatataa
     1021 tttaagcata ggagaaataa atggacaaag aatttattta taaacccgtt gatgaatata
     1081 aggaatacgc acgtcttcat cacgaaaata ttagtcaata ttttgatgaa ctaactaaaa
     1141 ctgctaatac ggatgttgga gctaatgaac taacggtaaa gaaaattaga actttagaaa
     1201 aagaaaatga tggtatacac aaacaacaat cacgttatca aatgttaaaa gtttttcttt
     1261 ggattttagt tgcaatagct atttttgtct ttttttatgc aatatatttg actgcaagtg
     1321 atgcagcagt tgaaactgta tcacttgtat taatgttcat tatttcagca attgtaattg
     1381 taggtggact tttattaata tataaagtta taaaacctaa atatttagtg ttggaagcaa
     1441 aaagtcaaga attggaaaga gaaatatcta atcaatataa agttgcctat caacaaatgg
     1501 gtagtttaaa tgctttgttt aaagttgaag taatagcaga actttttgaa aaaactattc
     1561 ctttgctaga ctttgataag atgttttcac aaaaaacact agagttttta aaaacaacat
     1621 ttggatttga tgattcaaca tctgaagaac aatcaacttt atatgttcaa tcagggcatt
     1681 taaatggtaa tccttttgct ttatttaaga atttatacca tgttttaggt acaaaaactt
     1741 atgtaggtac aaaaaccatt tcttggacag aaacgatagg gtcaggaaaa gatcgaagga
     1801 cagttacgag aacacaaaca ttaactgcga ctttagaaaa accttgtcct tactatagtg
     1861 aacaaacaca tttagttttt gcttctgaag ttgcacccga tttaacattt tatcgtaaac
     1921 aaggttatac tcatcgttta actcctaagc aagttgaaag aaaagttaga tctgatatgg
     1981 aagattttag agaagatact gaagataatt tctcaaaatc tattacttta cttgctaatg
     2041 atgaatttga agtattattt agagctttaa atcgtaataa tgaagttcag tttagattat
     2101 tatttacacc tttggcacaa caaaatatga ttgatttatt gcttgataag actgaaggct
     2161 ttggcgatga ttttgcattt gccaagaaaa ataaacttaa ctttatttcg cctgatcata
     2221 tgcaaggatt taaacttgat ataacaccta aacattttgt cgactatgat tataataggt
     2281 gtaaacaaaa ttggattaat tataatatgg aatattttag gaaaatgtat tttacttttg
     2341 caccaattct ttgtattcct ttatatcagc aacatccacc acatgaatat gtttataggg
     2401 atgttgtaga gagttattct tcattttttg aagatgaaat gattgtaaac aatttaggat
     2461 atgctaaatt tagtcatcca gattcaaaaa caaggaatat tttaaaaaca aaattagtca
     2521 atatcaatgg taaagttgag gatattcaag taactgctta tggatataac gtagagaaaa
     2581 gagttactta tatccccaaa actggtggag atggtagagt tcaccaagtt ccagttcatt
     2641 gggatgaata tttaccggtt gtacaaacta ctgatattgt aatggacttt tttgacgatt
     2701 tagataaaat ccaagaaatg atgagaaatc aagattttca aggagtttta gatcgaaata
     2761 tgtgtttagt taaatttaga ggcaaggagg aataaaaatg gctaatgaat taaacgaatt
     2821 aaaagattat gtcaatgaag aaggacgtga cattaatgta attgaaaaac aaataccagt
     2881 aaaagtaggc tggggttcaa ctctttttga agtgttatta tgggtttgtg ggattatccc
     2941 aggtttaatt ttcttgttta tgaaaattaa agctcaaaaa tactttgacc aattgcaaca
     3001 aaaaatccaa cacaatgcat cacaaattga taactactta gaacaaagag ttcaggtatt
     3061 acaaaacgtt gtgggtttag tagaaagatc aattgattta gataaagatg taatgaagtc
     3121 agttgcagct ttcagaggtg gaattcatcc aactgaagaa aatcgtagtc aagttgcatc
     3181 tcaattagaa tcag
//
LOCUS       DAOPNX010000141         3177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_33103_length_3177_cov_5.795324, whole genome shotgun sequence.
ACCESSION   DAOPNX010000141 DAOPNX010000000
VERSION     DAOPNX010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33103_length_3177_cov_5.795324"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..644
                     /locus_tag="PKN53_10605"
     CDS             69..644
                     /locus_tag="PKN53_10605"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOD89441.1"
                     /translation="MHEIFSRKKVVALEEYIVQLNGLDNKAYDFDFVVSDDFFTNFEN
                     SQIKKASLNAHLTLTKKPDSLELEISVLGSVTLTCDRCLDEYKENIDFKDTIFVEYGD
                     KTDFNAHEDFVLLKQGENSINVAQFIYEFAHFGLPLVHYHPNDENGKPTCNPEMLKIL
                     EKYSLVEGKYEENVDPWLSQLSDIKNSMLNN"
     gene            657..842
                     /gene="rpmF"
                     /locus_tag="PKN53_10610"
     CDS             657..842
                     /gene="rpmF"
                     /locus_tag="PKN53_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOD89442.1"
                     /translation="MAHPKRKTSKQRKNKRRTHYKASLPTLAKCSNCGATVEYHHVCP
                     ECGYYRGRLAISKEVIS"
     gene            848..1801
                     /locus_tag="PKN53_10615"
     CDS             848..1801
                     /locus_tag="PKN53_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HOD89443.1"
                     /translation="MKIGIDIMGGDFAPIQTLKGTILAKKELDDQDKLVLFGPKNIIS
                     EKLIEHGENVADYQIVDCSQTIEMSEDPVKAFIAKTDSSITKGFYYLQNDLIDGFASA
                     GSTGAMLVGATKVIGLIDGVLRPCISSHYPNDEGQDNLILDVGINSDSKPEQLVQFAQ
                     LGSLFAKHIMHIDNPKVALLNIGEEENKGNSVVKEAYQLLKNESSINFVGNVEGGDLY
                     KNSRVNVIVCNGFTGNIVLKLAESFYNILADRNIKDPFFEGFNYENFGGTPILGTKKT
                     IIVGHGKSSDIAIKNMILLTGKIIKSKLCEKTIKTFNYDKN"
     gene            1788..2807
                     /locus_tag="PKN53_10620"
     CDS             1788..2807
                     /locus_tag="PKN53_10620"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOD89444.1"
                     /translation="MTKIRAAITGVGAYVPPYVLTNDELAKMVDTTDQWIMERIGIKE
                     RHILLEEGKATSDMATEAILQLLEKTGTHPDEIDMLLCGVVTPDMQFPATANIICDKV
                     GIKNAFGYDINAGCSSFLFTLVTAAKFVESGSHKKVIVVGADKMSSIVDYKDRNTSPI
                     FGDGAGAVLLEPCTEGNGLIDSIMHVDGSGRQHLHQVAGGSLRPATFETILRRQHFIY
                     QEGQPVFKAAVSNMADVSVRMMERYNITPETLKYLVPHQANMRIIDATAKRMGITREK
                     TMLTITKYGNTTSATIPLCLWEYEHELRPGDNIIIATFGAGFAWGSTYVKWAYDGSKF
                     AKPAE"
BASE COUNT         1142 a          475 c          568 g          992 t
ORIGIN      
        1 taatatttaa acaaacttta tagtaataat catttttttc attgttattt gaaatagttt
       61 ttataaattt gcacgaaatt ttttcaagga aaaaagtggt ggctttagaa gaatatatag
      121 tgcaactcaa tgggcttgat aataaggctt atgactttga ttttgttgtt tcagatgatt
      181 ttttcacaaa ctttgaaaac agtcaaatca aaaaagcaag tcttaatgca cacctaacat
      241 taacaaaaaa acctgattct ttagaattag aaataagtgt gttaggtagt gttactctta
      301 cttgcgaccg ttgcttagat gaatacaaag aaaatattga ttttaaagac acaatttttg
      361 ttgaatacgg tgataaaaca gattttaacg ctcacgaaga ttttgtttta ttaaaacaag
      421 gcgaaaattc aataaatgtt gctcaattta tttatgaatt tgcacatttt ggtttgcctt
      481 tagtgcatta tcaccctaat gatgaaaatg gcaagccaac ttgcaatcct gaaatgctaa
      541 aaattttaga gaaatatagc cttgttgaag gaaaatacga ggaaaatgtt gacccatggt
      601 taagtcaact tagtgatata aaaaattcaa tgttaaataa ttaaaatttt aaagagatgg
      661 cacatccaaa gcgtaaaaca tcaaaacaaa gaaaaaacaa acgtcgtact cattataaag
      721 caagtttacc aacacttgct aaatgttcaa attgcggtgc tacagtagaa tatcatcatg
      781 tatgtccaga atgtggatac tacagaggca gacttgcaat cagtaaagaa gttataagct
      841 aattttagtg aagattggta ttgacataat gggcggggac tttgctccca ttcaaacatt
      901 aaaaggaaca atcctagcaa aaaaagaatt agacgaccaa gacaaattag ttctttttgg
      961 tcctaagaat attatttctg aaaagctaat tgaacatggc gagaatgtgg ctgactacca
     1021 aatagttgac tgttctcaaa ccattgagat gagcgaagac ccagttaaag catttattgc
     1081 caaaaccgac tctagtatta ccaaaggatt ttattattta caaaatgatt taatcgatgg
     1141 ctttgcttct gctggaagta caggtgctat gcttgttggg gcaacaaaag ttataggttt
     1201 aattgatgga gttttaagac catgcatttc ttcacattat ccaaatgatg aaggacaaga
     1261 taatttaatt ttagatgttg gcataaactc cgactctaaa cctgaacaat tagttcaatt
     1321 tgcacaactt ggcagtttgt ttgccaagca tattatgcat attgacaatc ctaaagttgc
     1381 actgctaaat attggtgaag aagaaaacaa aggcaatagt gttgttaaag aagcatatca
     1441 attacttaaa aacgaaagtt ctataaattt tgttggtaat gttgaaggcg gcgatttata
     1501 caagaactct agagttaacg taatagtttg caatggcttt acaggaaata tagttttaaa
     1561 acttgcggaa tcgttttaca atattttagc tgatagaaat attaaagatc cgtttttcga
     1621 aggttttaat tacgaaaatt ttggtggcac accaattctt gggacaaaga aaaccattat
     1681 tgtaggacat ggaaaaagta gcgatatagc tataaaaaac atgattttac ttactggaaa
     1741 aatcattaaa tctaaacttt gcgaaaaaac aattaaaacg tttaactatg acaaaaatta
     1801 gagcagcaat cacaggcgta ggtgcgtatg ttccacctta tgtgttaact aatgacgagt
     1861 tagcaaaaat ggttgatacc actgaccagt ggataatgga acgcataggc ataaaagaaa
     1921 gacatatact tttagaagaa ggtaaagcaa cttctgatat ggcaacagaa gcgatacttc
     1981 aacttttaga aaaaacagga acacaccccg acgaaattga catgcttctt tgtggtgtgg
     2041 ttacacccga catgcaattt cctgcaacag caaatattat ttgcgataaa gttggaataa
     2101 aaaatgcttt tggctatgat ataaatgctg gttgttcgag cttcttgttt acactagtta
     2161 cagcagctaa atttgttgaa agtggctctc acaaaaaagt aattgttgtt ggagcagaca
     2221 aaatgtcttc aatagttgac tataaggata gaaacacatc tcctattttt ggtgatggtg
     2281 ctggtgctgt tcttttagaa ccttgtactg agggcaatgg cttaattgat tctatcatgc
     2341 acgttgatgg ttctggtcgt cagcatttac atcaagtagc tggtggctca ctaagacctg
     2401 caacttttga aactatttta agacgtcaac attttattta tcaagaagga caacctgtat
     2461 ttaaagcagc ggtttctaat atggctgatg tttcagttag aatgatggaa agatataata
     2521 ttactcctga aacattaaaa tatttagttc cacaccaagc aaatatgaga ataattgacg
     2581 caacagctaa aagaatgggt attactagag aaaaaaccat gcttactatt acaaaatatg
     2641 gtaatactac ctctgcaact attccattgt gcttgtggga atatgaacac gaactaagac
     2701 caggtgacaa tattattatt gctactttcg gtgctggatt tgcttggggt tctacttatg
     2761 ttaagtgggc gtatgatggt agtaaatttg ctaaaccagc agaataacta ttttttaaaa
     2821 gtcggtgtct tataagcgtc cacctgcttc gttgctttcg atattcaaac tcagtcacgt
     2881 acttagtacg ctccttcgtt ttcaatctca gatgccttgc atttgaacac ttctaagaca
     2941 ccttgaaagt ttttgtacgt aggataacaa aataagtata ggtataaaat ttttacaaga
     3001 cacattttta ataataccca gacacacaaa ataagggtag tatcgatagc tttaataaaa
     3061 attgttgttt tttgcacaga aaacaagaaa aaatcagctt aagtgtgtaa aaaattacta
     3121 aatcttaaaa ctcatactaa tttgtgaaag aacatataga taaaatatag ataaaat
//
LOCUS       DAOPNX010000142         3158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_33388_length_3158_cov_6.677731, whole genome shotgun sequence.
ACCESSION   DAOPNX010000142 DAOPNX010000000
VERSION     DAOPNX010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33388_length_3158_cov_6.677731"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..565)
                     /locus_tag="PKN53_10625"
     CDS             complement(122..565)
                     /locus_tag="PKN53_10625"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOD89445.1"
                     /translation="MNSEHEKYMRIALQEAQKALDKDEVPIGAIVVCAGKIIGRGHNQ
                     TEMLNDVTAHAEMLAITAAENYLGAKYLKDCTLYVSLEPCVMCAGALAWSQISNVVYG
                     ASDSKRGFLKHGKEILHPKTNIIGGILAEECGDIITGFFVKKRKN"
     gene            complement(572..1060)
                     /gene="folK"
                     /locus_tag="PKN53_10630"
     CDS             complement(572..1060)
                     /gene="folK"
                     /locus_tag="PKN53_10630"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOD89446.1"
                     /translation="MNDVYLGIGGNLGNRLENIKKTLALISEKIGKIEKTSSVYLSEP
                     WGFEHKKYFTNLVVKLKTDKSPNKILELISEIELELKRLRTSSQYDARTVDIDILFYN
                     NEIIKTENLEIPHPRIQDRLFVLLPLVEIEKDLYHQSFKKSILELLNECKDKSRIRKL
                     NC"
     gene            complement(1053..1955)
                     /locus_tag="PKN53_10635"
     CDS             complement(1053..1955)
                     /locus_tag="PKN53_10635"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HOD89447.1"
                     /translation="MKKHKNIKLPQPSFLSFEPTNYCNLKCKLCVSGSGKLTRPKGFM
                     DFEMYKNVIDKNKKYLINILLHLQGEPLLHNQIVEMIAYAKANNIFTELSTNANILPS
                     VFENLKENMPDKLIVSLDGLTQETYSKYRVNGELQKVLEALELLSKLSKDKRPYVELQ
                     FLVFSHNENQIPELKSLKSKYKIDKISLKTAQIYDESEIDILPKNKKYSRYKIVNGKI
                     VIKSKLKNACKRIVFGSVITWDGKLLPCCFDKDADFICGDINTSSVNAVRNSNNYLMF
                     VKNVFLHRQNIEMCKNCTEGLKNE"
     gene            complement(2048..2809)
                     /locus_tag="PKN53_10640"
     CDS             complement(2048..2809)
                     /locus_tag="PKN53_10640"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HOD89448.1"
                     /translation="MDFSITVILLLCLAYFVAGFIDSIAGGGGLITLPSLLFAGVPPQ
                     FALGTNKMASTLGTGVAVVNFWRNKKINFKLILSGIGFAMLGAFLGSKIILSLDQSVV
                     GKIIIFLLPVAVVAVLLPRTKKSDAKALTKIDYFVIVPIICLVIGFYDGFFGPGTGSF
                     LTLSLFFFTKLKLIEATANAKVFNVMSNVGSLVAFILGGKVLFLLAIPFALSNMLGNF
                     LGSRLAIKSGDRIIKYFLILSLSILLISLVWKYYF"
     gene            complement(2907..>3158)
                     /locus_tag="PKN53_10645"
     CDS             complement(2907..>3158)
                     /locus_tag="PKN53_10645"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOD89449.1"
                     /translation="VQGAKYTDMQMDSMEKQFGRKIEEKDRDVYATIGGTPHLDTQYT
                     VFGEVVKGLEVIDKIASVKTYPGDRPVEDVKILKVSVIK"
BASE COUNT         1065 a          556 c          384 g         1153 t
ORIGIN      
        1 aaagttgaac cgagtttacg agctcaccga aggtaaagtg gctgacgcgt ctattgcttc
       61 gcagagttcg caagctcact ctgcttgcaa taacaatact tcagttaaaa aattcgcaat
      121 tttaattctt ccttttcttc acaaaaaaac cagttataat atctccgcac tcttctgcta
      181 atattccacc aataatattt gtttttggat gaagaatttc ttttccatgt tttaaaaagc
      241 ctcgttttga gtcggatgct ccataaacaa cattagaaat ttgcgaccac gctaaagctc
      301 ctgcacacat tacacaaggt tcgagactaa catacaaagt acagtctttt aaatattttg
      361 ctcccaaata attttctgct gctgtaatag caagcatttc tgcatgagct gtaacatcat
      421 tcaacatttc tgtttgatta tgtcctcttc ctataatttt tcctgcacaa acaactatag
      481 ctccaatagg cacttcgtct ttatctaagg ctttttgtgc ttcttgcaaa gcaatcctca
      541 tgtatttttc gtgttcgcta ttcatagtgt ttcaacaatt taattttcta attctactct
      601 tatctttaca ctcattcaaa agttccaata tgcttttttt aaaactttga tgatataaat
      661 ctttttctat ttcaaccaaa ggtaacaaaa caaaaagcct gtcttgtatg cgtggatgcg
      721 gaatttctaa attttctgtt tttataattt cattattgta aaacaaaata tcaatatcca
      781 ctgtccttgc atcatattgg gaagaagttc taagcctttt taattccaat tctatttctg
      841 aaattagctc caatattttg tttggtgatt tatctgtttt taatttgact acaagatttg
      901 taaaatattt tttgtgttca aaaccccaag gctcagagag ataaaccgaa gatgtttttt
      961 caatttttcc aattttttcg gaaattaaag ccaaagtttt tttgatgttc tcaagcctat
     1021 ttcctaaatt tcctcctatt ccaagataaa catcattcat ttttcaaacc ttcagtacaa
     1081 tttttacaca tttctatgtt ttgtctatgc aaaaatacat ttttaacaaa cataagataa
     1141 ttatttgagt tgcgaaccgc atttaccgaa gaagtattaa tatctccaca aataaaatca
     1201 gcatctttat cgaaacaaca aggcaacaat ttcccatccc aagtaattac agagccaaaa
     1261 actattcttt tacacgcatt tttaagtttt gattttataa caatcttgcc gttgacaatt
     1321 ttatatcgag aatatttttt attttttggc aaaatatcaa tttcagattc atcataaatt
     1381 tgagcagttt taaggcttat tttatcaatt ttatactttg attttaatga ttttaattct
     1441 ggaatttgat tttcgttatg cgaaaaaact aaaaactgca attccacata aggacgtttg
     1501 tctttactaa gtttggacaa taactccaag gcttctaaaa ctttttgcaa ttctccattc
     1561 actctatatt tagaatatgt ttcttgagtc aaaccatcta acgacacaat tagcttgtcg
     1621 ggcatatttt cttttaagtt ttcaaaaacc gatggtaaaa tatttgcatt tgtagaaagt
     1681 tctgtaaaaa tattatttgc ttttgcatat gctatcattt ccacaatttg attgtgtagc
     1741 aaaggctcac cttgaagatg caataaaata tttattaaat actttttatt tttatcaatt
     1801 acatttttat acatttcaaa atccataaaa ccttttggac gagtaagttt tccgcttccc
     1861 gatacacaaa gtttacattt taaattacaa taatttgtgg gttcaaaaga taaaaatgaa
     1921 ggctgaggaa gttttatgtt tttgtgtttt ttcacacaaa atttcaattt taaaaaaacg
     1981 aaatataatt ttatgaaatt taaaattttt cttgagttta aatattttac taatctcaat
     2041 cctaacatta aaaataatat ttccaaacta aacttataag caatatgcta agcgacaaaa
     2101 ttaaaaaata ttttattatt ctatctcctg attttattgc taatctactt cctaaaaaat
     2161 tgcctaacat attagataat gcgaaaggaa ttgcaagtaa aaacaaaact tttccgccta
     2221 aaataaaagc cactagactt ccaacatttg acataacatt aaaaactttt gcattggcag
     2281 tagcttctat taatttaagt tttgtaaaga aaaacaaact taaagtcaaa aaagagccag
     2341 ttcctggtcc aaaaaaaccg tcataaaatc ctattactaa gcaaattata gggacaatta
     2401 caaaatagtc gatttttgtt aatgcttttg catctgattt ttttgttcgc ggtaaaagta
     2461 ctgccacaac agcaactggc aaaagaaaaa taataatttt tccaacaaca ctttggtcta
     2521 aagataaaat aatttttgag cctaaaaacg cacctagcat tgcaaaacca attcctgaga
     2581 gaataagttt aaaattgatt tttttatttc tccaaaaatt tactacagct actcctgttc
     2641 caagagtgct agccatttta tttgttccaa gagcaaattg tggtggcaca ccagcaaata
     2701 aaagcgaagg taaagtaatt aaaccgccac ctccagcaat actgtcgata aatccagcta
     2761 caaaatatgc taaacagagc aaaagtatta cagtaatgct aaaatccatg acaaattttt
     2821 tgcaaaacta attattttca caatatagtt ttgtgatttt tattttaaaa ttaagaaaga
     2881 aattgttttt ttagtgatta aaaaaattat tttatcacac tcactttcaa tattttaaca
     2941 tcttcaactg gtctatcgcc aggataagtt tttacgcttg caattttatc aattacttct
     3001 aatcctttca caacttctcc aaatactgtg tattgagtat ctaaatgagg agttccgcca
     3061 atagtagcat aaacatctct gtctttttct tcaatttttc taccaaactg tttttccatg
     3121 ctatccattt gcatatcggt atattttgca ccttgaac
//
LOCUS       DAOPNX010000143         3111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_34066_length_3111_cov_3.532068, whole genome shotgun sequence.
ACCESSION   DAOPNX010000143 DAOPNX010000000
VERSION     DAOPNX010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34066_length_3111_cov_3.532068"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(508..2331)
                     /locus_tag="PKN53_10650"
     CDS             complement(508..2331)
                     /locus_tag="PKN53_10650"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD89450.1"
                     /translation="MKIKFIITILYVFFSQLCLAQWQGSGTEEDPFRIYNLNDLHLIK
                     ENEESFNGYAGIHFNLMNDIDDVLSFNIASNFRGYFHGKGHYITLGIDEETQLSDYFI
                     SQVYGTIDSLSFEGVFNYISLFNRIEITGKLSHITCYVNYIWDQGEAYIFCHQNSGTI
                     EYCVNNSDIESYFNAYISIFGYENESTGILNSCINNGNITYNGTIDVLISAAFTSNLN
                     KIINCINNGNIIVTNTPETVNVIAFGLTTGDTTTCSEFINCLNTGDFSVKNATAFGGI
                     THSGHGGSRSKLKALNCMNTGNLSGADKIGGIYYSYIHPDNGSLDINNCVNAGFINGS
                     VSSGGIIALEGSDIICPITVRNNLNLGKTNHYSLFAWSEQPNLEAFENNFYDKQLLTQ
                     MSSPQGDILENNAAKGLLTTDITGFALQDILGDGWSYAEGRYPVPLGLENHPAALLAA
                     TPVYLPYTDQDNYNTVDSVTCHFLLGTENNVEWECSSPTVNLEETEDGLKGVLQSSGY
                     ANLVANLGGFHKNIILNIKSVCEPSYKNEEIQKTQAIAYPNPTKDILYFNQASAYEIY
                     DLQGKLILKSKKPQNSVNTSKLKSGIYLIKIGKEIMKFVVE"
     gene            complement(2334..>3111)
                     /locus_tag="PKN53_10655"
     CDS             complement(2334..>3111)
                     /locus_tag="PKN53_10655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD89451.1"
                     /translation="LPINEVKRCVLQIDISNYNNSILKSMDDYDIVNINPLYPTHPPY
                     YTKLHPMGYMNALIFNNLYYDVIGFGSLYNLYSYLVEIMDLNNSIDGCEHEVDLITYI
                     PDILVENFTPSPTHHTFALQQYAPATITGQYALTWETLCSHPSKSTVYFANNRKEHIQ
                     RIIDENMKKYPQKKSLLEKDNYINYPKIQVFNNKFICIDFEGECKFNIYDFNGRIVQT
                     GITQNYELNNIEIKTNGMYLIRITDDSNNTISNKIIITNN"
BASE COUNT         1014 a          495 c          463 g         1139 t
ORIGIN      
        1 aagtggcgca actttattaa ttaaaagagt ttttagtgtt gagtttttag tttttagtag
       61 tgagttttaa aaacttcaca ctgatagaat ttctttagtg caagatttgt atatacttgc
      121 ttcgcagagt tcgcaggctc actctactcg caagagttag tcttaaacca caaaagacac
      181 aaaaggacac aaaagttaaa agcggcttgc gggttcggca tttttttatg aactttaaac
      241 tttataaact ttttaactga tttattgtgt ttagttttta gtagtgagtt ttaaaaactt
      301 cacaatgata aaaattcttt agtgcaaggt ttgtatatat ttgcttcgca gagttcgcaa
      361 gctcactctg ctcgcaatta caatactcag aacgctcgct cggatttata atccgagtgc
      421 ttggaattgt ttcactcgga ttgcaaatcc gagcgagcga ggtaagtggt aggggtcaac
      481 gaagctaaat ccgcacgagc ggaggtttca ctccactaca aacttcatta tttcttttcc
      541 aatttttatt agataaattc cagattttag ttttgatgta tttacagaat tttgaggttt
      601 tttgcttttc agaattagtt tgccctgcaa atcgtaaatc tcataagctg aagcttgatt
      661 aaaataaagt atatcttttg ttgggtttgg atatgctata gcttgagttt tttgaatttc
      721 ttcgttttta taacttggct cgcaaacaga ttttatattt aagataatgt ttttatgaaa
      781 accacctaaa tttgctacta aatttgcata acctgatgat tgcaaaactc cttttagtcc
      841 gtcttcggtt tcttctaaat taactgtagg agaagaacac tcccattcta cattattttc
      901 tgtaccaagc aaaaaatggc aagttacact atcaactgta ttgtaattat cttggtcggt
      961 gtaaggcaaa tatactggcg tggcagcaag tagtgcagca ggatgatttt ctaatcctaa
     1021 aggaacagga taacgacctt cggcataaga ccaaccatcg ccaagaatat cttgtaaggc
     1081 aaaaccagta atatctgtag ttaacaaacc ttttgcagca ttattttcta aaatatctcc
     1141 ttgaggacta gacatttgag taagtaattg tttgtcgtaa aaattgtttt caaaagcctc
     1201 taaattaggc tgctcactcc aagcaaataa agaataatga tttgttttac caagatttag
     1261 attgtttctt actgttattg gacatattat atctgaacct tccaatgcga taataccacc
     1321 tgaagaaaca ctcccattta taaaacctgc attaacacaa ttgttaatat ccaaagaacc
     1381 attatctgga tgtatatatg aataatatat tcctcctatt ttatcagctc cgcttaaatt
     1441 tcctgtattc atacaattca aagcctttaa tttggatcta cttccaccgt gtccagagtg
     1501 agtaatacct ccaaaagcag tagcattttt tacagaaaaa tcaccagtat ttaaacaatt
     1561 tataaattca ctacatgtag tagtgtctcc agttgttaat ccaaatgcta tcacattcac
     1621 tgtttcggga gtatttgtaa cgattatatt accattattt atacaattta ttattttgtt
     1681 caaattgctt gtaaatgctg cagatataag tacatctata gttccattat atgtgatgtt
     1741 accattgttt atacatgaat ttaaaatgcc tgtactctca ttttcgtacc caaatataga
     1801 aatataagcg ttaaaataac tttcaatatc agaattgttt acacagtatt caattgttcc
     1861 agaattttga tgacaaaata tatacgcctc cccttgatcc caaatgtaat taacataaca
     1921 agttatatgt gaaagttttc ctgttatttc aatacgatta aataaactta tataattaaa
     1981 cactccctca aaacttaagg agtctattgt gccgtaaact tgagatataa aatagtctga
     2041 aagttgtgtt tcttcatcta ttcctaaagt tatataatgt ccttttccat gaaaatagcc
     2101 ccgaaaattc gaggctatat taaaagacaa aacatcgtca atatcgttca ttaaattaaa
     2161 gtgaataccc gcataaccat taaaactttc ttcattttct tttattaggt gtaaatcatt
     2221 taagttgtaa atgcgaaatg ggtcttcttc tgtaccactg ccttgccatt gagctaaaca
     2281 cagttgagaa aaaaatacat ataatatagt aattataaat tttattttca taattagttg
     2341 tttgtaataa taattttatt gctaattgta ttattgctgt catctgtaat tctaatcaaa
     2401 tacataccat ttgttttgat ttcgatattg tttagttcat agttttgggt tattcctgtt
     2461 tgcacaattc ttccattaaa gtcgtaaatg ttaaatttac attctccttc aaaatctatg
     2521 caaataaatt tattattaaa aacttgtatt tttggataat taatataatt atctttttct
     2581 aataaggatt tcttttgtgg atattttttc atgttctcat caataattct ttgaatatgc
     2641 tcttttctat tattagcaaa atatactgtt gattttgaag gatgtgagca aagagtttcc
     2701 caagttaaag catattgccc tgtaattgta gccggtgcat attgttgtaa agcaaatgta
     2761 tgatgagttg gtgaaggtgt aaaattttcc acaagtatgt ctggtatata agtaattaaa
     2821 tctacctcat gctcacaacc atctatacta ttatttaagt ccataatttc tactaaatag
     2881 ctatataaat tataaagaga tccaaaacca atgacatcat aatatagatt gttaaaaatc
     2941 aatgcattca tatagcccat agggtgtaat tttgtataat aaggaggatg tgtgggataa
     3001 agtgggttta tgtttacaat atcatagtca tccattgact ttaaaataga gttattataa
     3061 ttagatatat caatttgtag aacacatctt ttaacttcgt ttattggcaa c
//
LOCUS       DAOPNX010000144         2681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_41409_length_2681_cov_6.268850, whole genome shotgun sequence.
ACCESSION   DAOPNX010000144 DAOPNX010000000
VERSION     DAOPNX010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41409_length_2681_cov_6.268850"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2439
                     /locus_tag="PKN53_10660"
     CDS             <1..2439
                     /locus_tag="PKN53_10660"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD89452.1"
                     /translation="QYVVYKETGTGLTITGLTNDETYYAKIFVRKGTDWSSGVELVLN
                     PKITTALGHGDLAILAVNTQSGFGDTGDEISIVSFKDIAPNTSLDFTDNGYERATAGK
                     WGDTEGTIRLTRIGEEITAGKVITFEIQGKTSDLPFVTNINIYIDGLLDNENWTAEKL
                     GNCNDFDLNKEDQLWIMQNGNWVNPSGTHDATYTGNVIYGWTATGWKTAPGYNNAKGS
                     TIYPGCECATTDVEALGSDFKSKVKYTGSLTATSKYEWIDRINDPANWTGWKTNAPYN
                     DSLPDYNYKGGIQLQITAGDAIESKWMGYKNSAWCNCANWYNLRVPNENTDVEITPHN
                     SLRFDLKLSAHPDSLAVCKDLIVKGDVYNAANASLKVKGNFTLDGGSVNFNTNAINFE
                     IGGDMSIDNVNSFKTSQANTKLNGTSIQNINGNSEVVFGTLTIDKPSAHVVLAKNIKV
                     SNINFIKGKINTGTNRVFIDNTATNAIGSISPLSYINGNLRRAVASTGSYTLPVGNDS
                     NFELATVELNSSSGLTHLDARFDSHVTSTDISSLNLFVDGTILQSLLDAGYWTIAPNS
                     GLSSINYDISLDMRGATNAGAQAGQHTVIKRDNSSSNWYLQGNHTNATQSISGGSVHA
                     VRTGLTSFSEFSIAKHNLFILPVELISFEANCNTDFVELNWITVSETNNDYFIVQRSQ
                     DATNWKNLSKIQGAGFSSSEKEYEYIDNTQVSGNMYYRLVQVDFDGTKTISNIVNVNC
                     INKFEIPNISIYPNPANDILNFSQAQTYEILDIQGRVLMQSKTKQNSVNISELKEGMY
                     FIKFENELLKFIKY"
BASE COUNT          980 a          380 c          472 g          849 t
ORIGIN      
        1 caatatgttg tttataaaga aactggtact ggactaacga taactggttt aacaaacgac
       61 gaaacttatt atgccaaaat atttgtacgt aaaggcacag actggtcaag tggagtagaa
      121 ttggtgctta atcctaaaat tactactgct ttaggacatg gcgatttagc tatattggct
      181 gtaaatacac aatctggctt tggagataca ggagatgaaa tttctatagt aagctttaag
      241 gatatagcac ctaatacatc attagatttt actgacaatg gttatgaaag agcaactgct
      301 ggaaaatggg gagatacaga agggacgatt agacttacaa gaataggcga agaaataact
      361 gctggtaaag taataacttt tgaaattcaa ggtaaaacta gtgatttacc ttttgttact
      421 aacattaata tatatattga tggtttgctt gacaatgaaa attggacagc agagaaactt
      481 ggaaattgta atgattttga tttgaataaa gaagaccagc tatggattat gcaaaatgga
      541 aattgggtaa atccatcagg aactcatgat gcaacatata ctggtaatgt tatttatggt
      601 tggacagcaa caggttggaa aactgcacca ggatataata atgcaaaagg ttctactatt
      661 taccctggtt gcgaatgtgc aacaacagat gttgaagcac ttggctctga ttttaaaagc
      721 aaggtaaaat atactggtag tttaacagca acaagtaaat atgaatggat agatagaatt
      781 aatgaccctg caaactggac tggctggaaa actaacgccc cctataatga tagcttacct
      841 gactacaact acaaaggcgg aatacagtta caaattactg ctggcgatgc aatagaatca
      901 aaatggatgg gctacaaaaa ttctgcttgg tgtaattgtg caaactggta taatcttaga
      961 gttcctaacg aaaatacaga tgtagaaata acaccacaca atagtttaag atttgatcta
     1021 aaactaagcg ctcaccctga ttctttggca gtttgtaaag atttaatagt aaaaggcgat
     1081 gtatataatg ctgcaaatgc ttctttaaaa gtaaagggca actttacttt agacggtgga
     1141 agcgtgaact ttaatactaa tgcaatcaat tttgaaattg gtggagatat gagtattgat
     1201 aatgtgaata gctttaaaac ttcacaagca aatacaaaac taaatggaac ctcaattcaa
     1261 aatatcaacg gaaatagcga agttgttttc ggaacattaa ctattgataa accttcagct
     1321 catgtcgttc ttgctaaaaa tattaaagta agcaatataa atttcatcaa aggaaaaatt
     1381 aacactggaa ctaatcgtgt ttttattgac aatactgcaa caaatgcaat tggaagcata
     1441 tcgcctttat cgtatataaa cggaaattta agacgagctg ttgcttcaac tggttcttac
     1501 acattacctg taggaaacga ttcaaatttt gaattagcaa cagtagaatt aaattcaagt
     1561 tcaggattaa ctcaccttga tgcgagattt gacagtcatg taacaagtac agatatttcg
     1621 tcattaaatc tttttgttga tggaactatt ttacaatctc ttttagatgc tggttattgg
     1681 acaattgccc caaattcagg attaagttca attaattatg atataagttt agatatgcgt
     1741 ggagcaacaa atgctggagc tcaagctgga caacatactg ttattaaacg cgacaactcg
     1801 tcaagcaatt ggtatttgca aggaaatcac acaaatgcaa ctcagtcaat ttctggtggt
     1861 tcagtacatg ctgtaagaac tggattaact tccttctcag aattttcaat tgcaaaacat
     1921 aatttgttta ttttacctgt tgaattaatt agttttgaag ctaattgcaa tacagatttt
     1981 gttgaattaa attggattac tgtaagcgaa accaataatg attattttat tgttcaaaga
     2041 agccaagacg caacaaattg gaaaaatcta agcaaaattc aaggagctgg attttcatca
     2101 tcagaaaaag aatatgagta tattgataat actcaagttt ctggaaacat gtattatcgc
     2161 ttagtacaag ttgattttga cggaacaaaa actatttcaa atattgtaaa tgtaaattgc
     2221 ataaataaat ttgaaattcc taatatttca atttatccaa atccagctaa tgatattcta
     2281 aactttagtc aagcacaaac ttacgaaatt cttgatattc aaggaagagt gctaatgcaa
     2341 agcaaaacaa aacaaaactc tgtgaatata agcgagttaa aagaaggaat gtactttata
     2401 aaatttgaaa acgaactttt aaaatttata aaatattaat gtttatttgc ttacttcatt
     2461 taataaaaaa cctgtaaccc tacaggtttt tttataagtt tactttgtgt ttttggggtg
     2521 tttcactttg tgttttgagg catatttaat caaccacttt ttacattttc agaataaaac
     2581 agatttttat atttttgtat agtttttgct acaaattggt agaagtttaa aaaatatttt
     2641 tatgaaaatt ggtcttcttt cagatactca tggatattta g
//
LOCUS       DAOPNX010000145         2614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_42835_length_2614_cov_6.128957, whole genome shotgun sequence.
ACCESSION   DAOPNX010000145 DAOPNX010000000
VERSION     DAOPNX010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42835_length_2614_cov_6.128957"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1994)
                     /locus_tag="PKN53_10665"
     CDS             complement(<1..1994)
                     /locus_tag="PKN53_10665"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89453.1"
                     /translation="MKNTKSLLLLSVIICFFSLSIKAQNWSETIKLVPSDRSKNSYFG
                     QSVDVYGNFAIVGAYFDKLGVNSGDPSLTNCGSAYILENVDGNWQIIQKICANDRAEQ
                     SKFGASVAIWEDYIAVGAIGKSEAPNTNLGVVYVFKNNAGVWTQINKLLPSDPENDCG
                     FGNSIDIENNTLIVGAENKKNGTAYNVGAVYIYELVNNSWSFSQKLMASDCSAGDFFG
                     CSVAISGEYIIAGAYAQDKNATGGGELTDAGATYIFKYNAGTWTQTQKLVANDRAQEN
                     YFGRAVDIDGDYAVISATYDSRNSLGVADKTRTGSAYVFKNNAGTWTQTQKILANDRS
                     NYDEFGNAVSIFNDKLIIGVSQQDFDSNGENSIFNAGAVYVYENVSSSWNFTQKLTAS
                     NRQSNDYFGNAVAIYNGQLLVGANQNKYDANENNYLAGAGAVYVFNNSPKIEIFQEET
                     LIANGGSFDFGEVGYSGDGLDLIFKIKNSGGTTLNLTGNPIIELVSGDANIFVINQNS
                     VQPSVLPNQETNFTIKFKPTSLGSFSAQISIASDCTSDNPYIISLQGVGGKSSQTITG
                     FADIPTKTYGDASFQVSAVASSGLDVVFTSSNNNVATCSGTNGSTITIVGAGVCQIYA
                     NQAGNANYYPAPQVAKTLNVNKRVINVTADALSKHYGNTDP"
     gene            complement(2153..>2614)
                     /locus_tag="PKN53_10670"
     CDS             complement(2153..>2614)
                     /locus_tag="PKN53_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89454.1"
                     /translation="KMKITRINRKTYQLTKNEQVLGQLTYKNSLSNKAEITLIDSAIY
                     QIKPAGFFKSHVIVTKNEIEIAKFKMNWKGQLVISLQFEREFFLKSKGFFHSKYIIEN
                     KDGEKIIQLEPKFNWSKFCYNYNISYNNQSQDKFFILLAVYCINHYRALVS"
BASE COUNT          798 a          453 c          404 g          959 t
ORIGIN      
        1 gggtcagtat ttccgtagtg tttactaaga gcatcggcag ttacattaat tactcttttg
       61 ttaacattaa gagtttttgc aacttgagga gcaggatagt aattagcgtt tcctgcttga
      121 tttgcataaa tttgacatac tccagctcca actattgtaa tggtgcttcc attagttcca
      181 ctacaagtag caacattatt attagaactt gtaaaaacta catctaaacc agagcttgca
      241 actgccgaaa cttgaaaact agcgtctcca taagttttag ttggaatatc tgcaaaacca
      301 gtgatggttt gagagctttt gcctccaact ccctgaagac taataatata tgggttgtct
      361 gaagtacagt cgctagctat tgaaatttga gctgaaaaac tgcctaaact tgtaggcttg
      421 aatttaattg taaaattagt ttcttgattt ggtagtactg aaggctgaac actattctga
      481 tttattacga aaatatttgc gtctccactt acaagttcta taattgggtt tcctgttagg
      541 tttagagtag tgcctccaga attttttatt ttaaaaatta aatctaagcc atcgccacta
      601 taaccaactt ctccaaaatc gaaactacct ccattggcaa ttaatgtttc ttcttggaaa
      661 atttcaattt ttggtgaatt attaaaaaca taaacagctc cagcacctgc taaataatta
      721 ttctcatttg cgtcatattt attttgattt gctcctacta ataattgtcc attatatata
      781 gctacagcat ttccgaaata atcattagat tgtctgttgc tggctgtaag tttttgtgta
      841 aaattccagc tgcttgaaac gttttcataa acataaacag ctcctgcatt gaaaatagag
      901 ttttctccgt tactatcaaa gtcttgttgt gagacaccaa ttatcagttt atcattaaaa
      961 atcgatacag catttccaaa ttcgtcataa ttgcttctat cattagccag aattttttgt
     1021 gtttgcgtcc aggttcctgc gttattttta aaaacataag cagaacctgt tcttgtttta
     1081 tctgcaacac ctaatgagtt tctagaatca taagtggcac ttataactgc atagtcgcca
     1141 tcaatatcta cagcacgccc aaaataattc tcttgggctc tatcgttagc aactagtttt
     1201 tgtgtttgtg tccacgttcc tgcattatat ttaaaaatgt aagtagctcc agcgtctgtt
     1261 agttctccgc ctccagtagc atttttatct tgagcataag cacctgcaat tatatattca
     1321 cctgaaattg caactgagca gccaaaaaaa tcacctgccg aacagtcgga agccattagt
     1381 ttttgtgaaa aactccatga attgttgaca agttcgtaaa tatatacagc tccgacatta
     1441 taagctgtgc catttttctt attttctgct ccaacaatta atgtgttgtt ttctatatca
     1501 atagagttgc caaagccaca gtcgttttcg gggtctgatg gaagtagttt gtttatttgt
     1561 gtccaaactc ctgcattgtt tttaaaaaca tacacaacac ctaaatttgt gttaggagct
     1621 tcagatttac ctattgctcc aacagcaata taatcttccc aaattgcgac agaagctcca
     1681 aatttgcttt gttcagctct atcgttagca caaatttttt gtattatttg ccagttgcca
     1741 tcaacatttt ctaaaatata agccgaaccg caattagtta agctgggatc tccagagttt
     1801 actcctaatt tatcgaaata tgctcccaca atagcaaaat ttccataaac atcaactgat
     1861 tgaccaaaat aactattttt acttctgtct gaaggaacta gctttatggt ttctgaccag
     1921 ttttgtgctt taattgataa gctaaaaaaa catataatta cgctaagtaa aagtaaagat
     1981 ttagtatttt tcataacaaa ataatttttg taatagacaa atatacgagt tttttttcat
     2041 atagagggta gtttttaggg aaaaaattga ttggaagaga ttttttttgt tttatcgcat
     2101 tacttagtgc ttgtgtgcaa tagtgtggct tatgaatatc tgtgttttgt tatcaactta
     2161 ctaatgccct ataatggttt atacaataaa ctgcaagtaa aataaaaaac ttgtcttgtg
     2221 attgattatt atatgaaata ttataattgt aacagaactt agaccagtta aattttggtt
     2281 caagttgaat tattttttct ccatctttat tttcaattat atatttactg tggaaaaacc
     2341 cctttgattt aaggaaaaat tccctttcaa attgcaaact aataactaat tgccctttcc
     2401 aattcatttt aaatttggca atttctattt cattttttgt aacgataaca tgtgatttaa
     2461 aaaaacctgc tggcttaatt tgataaatgg cagaatctat taatgtgatt tcagctttgt
     2521 tagaaagtga gtttttataa gtaagttgtc ctaatacttg ctcgtttttg gttaactgat
     2581 atgtctttct gttaatgcga gttattttca tttt
//
LOCUS       DAOPNX010000146         2597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_43211_length_2597_cov_6.001574, whole genome shotgun sequence.
ACCESSION   DAOPNX010000146 DAOPNX010000000
VERSION     DAOPNX010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43211_length_2597_cov_6.001574"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PKN53_10675"
     CDS             <1..275
                     /locus_tag="PKN53_10675"
                     /inference="COORDINATES: protein motif:HMM:NF019583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HOD89455.1"
                     /translation="SAWYIFSALGFYPVLPGSSDYAIGSPLVKNATIKLNNGKVFKIE
                     VENQSDENIYVKNVYINGKQITDFTLNHKDILKGGTLKFEMTNKMN"
     gene            521..1291
                     /locus_tag="PKN53_10680"
     CDS             521..1291
                     /locus_tag="PKN53_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018027942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOD89456.1"
                     /translation="MGYHNIEFKIDGTTAQITMNRPEALNALNSQVFVELNSALDEIE
                     KNETLRALIITGSGKAFVAGADIAEMSGMNEKLAFEFAKTGHNTFNRVANLKIPVIAA
                     INGFALGGGCEFAMACDIRIANTFAKFGQPEVNLGIIPGYCGTQRLSRIVGLSNALNL
                     ILTADMIDANEAFRLGLVQKVTEPEELLNEAYKLALKIASKGKLAVEKAKEVTVKGVN
                     MNFEAASELEIKEFSKLFGKHESKEGMTAFLEKRKPNW"
     gene            1337..>2597
                     /locus_tag="PKN53_10685"
     CDS             1337..>2597
                     /locus_tag="PKN53_10685"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOD89457.1"
                     /translation="MNYNERFENVTIVGAAGKMGSGILLLTAMEMYNISMKPENKDKD
                     YILFAFDISQNALSGLKTYIQDQLTKQAERKIEILKEHYKDKKDLANDSDIISQYVKD
                     VMAMIQPVTTIESAYESHIIFEAASENPDLKTKIFAQINANSKCEPWFFTNTSSIPIS
                     YLNEKANLNGRIIGVHFYNPPAVQKLVEVIKCDQTPDIMGEFVSTYIKNLRKIEVPSY
                     DKAGFIGNGHFMRDALYGMMRMENLRKDMSFAEAVYIVNKVTQDFLIRPMGIFQLIDY
                     VGIDVCQFIMKVMKPYVADEDIHSPLLDTMMEIGVKGGQNHDGSQKDGFLKYEKGRIV
                     AIWDLDKKEYVKIEDISEKCDKLIGALPQEHQPWKVVVRDPNKNEFLTKYFSALQKEN
                     GMGAKLAVEYHIKSKQIGEKLVSDKVAF"
BASE COUNT         1007 a          346 c          435 g          809 t
ORIGIN      
        1 tgagtgcttg gtacattttt agtgctttag gattttatcc agttttaccc ggaagcagtg
       61 attatgcaat tggcagtcct ttagttaaaa atgcaactat aaaacttaat aacggcaaag
      121 tttttaaaat agaagttgaa aaccaatctg atgaaaatat ttatgtaaaa aacgtttata
      181 ttaatggaaa acagattact gattttactc taaatcataa agatatacta aaaggaggaa
      241 cattaaaatt tgaaatgacc aataaaatga attaagaatt aagaattgaa gaattaagaa
      301 ttaaaaattt ataaatcagt taaaagtgtt tagttaaact ttatgaactt tgctttcagc
      361 gagctcgtaa actcggttga acttaatgaa ctttacgaac tttacgaact ttataaactt
      421 ttaacttatt tcaatgcctt ttcaaatttt attttttctt gcaaattaaa aaaaatatat
      481 tatctttgaa atctaatgtt tatatttaaa aaagttattt atgggatatc ataatattga
      541 atttaaaatt gacggaacta ctgctcaaat tactatgaac agacctgaag cacttaatgc
      601 tttaaactct caagtttttg ttgagcttaa ttctgctttg gacgaaattg aaaaaaacga
      661 aactttaaga gctttaatca ttactggaag tggtaaagct tttgttgcag gagctgacat
      721 tgctgaaatg tcgggaatga acgaaaaact agcttttgag tttgcaaaaa ctgggcataa
      781 cacttttaat agagttgcta acttaaaaat tcctgtgatt gcagctatta atggtttcgc
      841 tttaggcggt ggttgcgagt ttgcaatggc ttgcgacatt agaattgcaa atacttttgc
      901 aaaatttggt caaccagaag ttaatttagg cataattcct ggatattgtg gaacacaacg
      961 cttatccaga attgtaggtt tatcaaatgc cttaaatcta atattaactg ccgatatgat
     1021 tgatgcaaac gaagctttta gattaggttt agtacaaaaa gttacagaac ctgaagaatt
     1081 gctaaacgaa gcctataaac ttgctttaaa aattgcttcc aaaggtaaat tagcggttga
     1141 aaaagctaaa gaagttactg taaaaggtgt aaatatgaat tttgaagctg cttctgagct
     1201 tgaaataaaa gaattttcta aactttttgg caaacatgaa tctaaagaag gaatgacagc
     1261 atttttagaa aagagaaaac ctaactggta aataataaac taaaataata tttttaaatt
     1321 taataaaaaa accaacatga attacaacga aagatttgaa aatgtaacaa tagttggagc
     1381 tgcaggaaaa atgggtagcg gcatccttct gttaactgct atggaaatgt acaatattag
     1441 catgaagcct gaaaataaag acaaagatta cattttattt gcctttgata tttcacaaaa
     1501 tgctttgtca ggtttaaaga cttacattca agaccaactt acaaaacaag ccgaaagaaa
     1561 gattgaaata ttaaaagaac actataaaga caaaaaagat ttagcaaatg attcagacat
     1621 aatctctcaa tatgtaaaag acgtaatggc tatgattcaa ccagttacaa caatagaatc
     1681 agcttatgaa tcacatataa tttttgaagc cgcaagtgaa aatcctgatt taaaaacaaa
     1741 aatttttgct caaattaatg caaattcaaa atgtgaacct tggtttttca caaacacatc
     1801 atcaattcca atttcttact taaacgagaa agcaaatttg aatggcagaa ttattggagt
     1861 tcatttttac aatccaccag cagtgcaaaa attggttgaa gtaattaaat gcgaccaaac
     1921 cccagatata atgggagaat ttgttagcac atatatcaaa aatttaagaa aaatagaagt
     1981 cccatcatac gacaaagcag gatttattgg caatggacat ttcatgcgtg atgcactata
     2041 cggaatgatg agaatggaaa atttaagaaa agatatgagt tttgctgaag cagtttatat
     2101 tgtaaataaa gttactcaag attttttaat tcgtcctatg ggaattttcc aacttattga
     2161 ttatgtggga attgatgttt gccaatttat tatgaaagtt atgaaaccat acgttgcaga
     2221 cgaagatatc cacagtccgc ttttggatac tatgatggaa attggagtta aaggtggtca
     2281 aaatcatgac ggttctcaaa aagacggttt cttaaaatat gaaaaaggta gaattgttgc
     2341 aatttgggac ttagataaaa aagaatacgt aaaaattgaa gatatttctg aaaaatgtga
     2401 taaacttatt ggagctttac ctcaagaaca ccaaccatgg aaagttgttg taagagaccc
     2461 taataaaaat gaatttttaa ctaaatattt ttctgcttta caaaaagaaa acggaatggg
     2521 tgctaaactt gcagtagaat accatataaa atctaaacaa attggcgaaa aacttgtatc
     2581 agacaaagtt gcattca
//
LOCUS       DAOPNX010000147         2559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_44034_length_2559_cov_5.642572, whole genome shotgun sequence.
ACCESSION   DAOPNX010000147 DAOPNX010000000
VERSION     DAOPNX010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44034_length_2559_cov_5.642572"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..1310)
                     /locus_tag="PKN53_10690"
     CDS             complement(21..1310)
                     /locus_tag="PKN53_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOD89458.1"
                     /translation="MKNNLPLLQNADLRGKIVLVRVDHNVVKSGLIHDPYRIDSTFGT
                     ISYILANGGKPILMTHVGRPKDKATKSIIISEKTSVQPIVNYLKEKLQLRIEVPEFTA
                     SETQGYLSVDTSVNHLLKKLKNNEIDMIYLPNTRWFLGEESKGEEQDKFAYQLAGLAD
                     VYVNDAFGSWQAHASTVGVNKYLPSYTGFLMQKEIKNLERLFEAEKPLLAVVAGSKFD
                     TKINSLSALIKRADKLVIGGVLYNAYLCAKYDISIKGVGEDDVKLAKEFYEFSKQYPN
                     KIVELPYIIESETMDGKFEGKYKTIDVRTLKPKTELGYVLDAAPESFKEKGVQDTFMQ
                     AKTIFVNAVMGFTPHFNDGTIAMDSLIDQNKDALKLYGGGDTLQELKQLLPGIYLSAL
                     DNPKYYMFTGGGAVLTAIEEGTPNGIPPIQELLKNNN"
     gene            complement(1452..>2559)
                     /locus_tag="PKN53_10695"
     CDS             complement(1452..>2559)
                     /locus_tag="PKN53_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89459.1"
                     /translation="FIILIFCFCLQNKFALGQNTWDKVDSLIINGKLNPKVQKYYSTI
                     YKAEDAIVKEKFAKAAKIYEKAFSGSVIPFRYDLKNAIQCELKSSPNKQNMFRYVNLL
                     IRKGDKKEKFYNDSSLLALDFWQELKNVIDTSVCLIDSSTIKFMSFLIENDQDYRRQC
                     NEKYDGNTYNEFTIDSINIIDSLNYERILNFIKNNDIISEEVFGNFQAISLIITHNRH
                     RPQAYELLYNDVINGNLDTRAYYDELQLTGYRFNGVYTLGKDSHGWILGTQGTQVYFY
                     HTESTPEKFNIKKIDKYRKKLYCDKVVSFHKKKAWGFSHGFEFFRCWLTEQIMTESQL
                     AKIIKSDENRKNRNIIFNDESVREYYYNLLEEQE"
BASE COUNT          825 a          437 c          324 g          973 t
ORIGIN      
        1 aaaactttat aaacttttaa ctaattgtta ttttttagta attcttgaat aggcggaatt
       61 ccattaggcg ttccttcttc aatagctgtt aaaactgctc caccaccagt aaacatataa
      121 tattttggat tatctaaagc acttaaataa attcctggta aaagttgttt taattcctgt
      181 aaagtatctc cacctccata aagttttaaa gcatctttat tttggtctat taagctatcc
      241 atagcaattg tgccatcgtt gaagtgaggt gtgaatccca taacagcatt aacaaaaatt
      301 gtttttgcct gcataaaagt atcttgcact cctttttctt taaatgattc tggtgcagca
      361 tctaaaacat aacctaattc tgtttttggt tttagtgtgc gaacatctat agttttgtat
      421 tttccttcaa atttgccgtc catagtttcg ctttcgataa tgtatggaag ctctactatc
      481 ttgttaggat attgttttga aaattcgtaa aattcttttg ctaatttaac atcatcttcg
      541 ccaacgcctt tgattgaaat atcatatttt gcacataaat aagcattata caaaactcca
      601 ccaattacta atttatctgc tcttttgatt aaagcagaaa gagaatttat ttttgtatcg
      661 aatttactac ctgcaacaac tgctaacaaa ggtttttctg cttcaaataa tctttctaag
      721 tttttaattt ctttttgcat taaaaagcct gtgtaagatg gtaaatattt atttactcca
      781 actgtagaag catgagcttg ccaagagcca aaagcatcgt ttacataaac atcagctaat
      841 cctgctaatt gatatgcaaa tttatcttgt tcttcgcctt ttgattcttc gcctaaaaac
      901 caacgcgtat ttggcaaata aatcatatca atttcattat tttttagttt ttttagaaga
      961 tgatttacgc ttgtatcaac acttaaatat ccttgtgttt cggaggcggt aaactcagga
     1021 acttctattc ttaactgtaa tttttctttt aaataattta ctattggttg cactgaagtt
     1081 ttttcgctta taattatact ttttgttgct ttatctttag gacgaccaac atgagtcatt
     1141 aaaattggtt tgccaccatt tgctaaaata taacttattg taccaaaagt tgaatcaatt
     1201 cggtatgggt cgtgtattaa accagatttc acaacattgt ggtcaacacg aactaaaact
     1261 attttgcctc ttaaatcagc attttgtaaa agtggaagat tatttttcat gactttaaat
     1321 tttaattttc cacaaaaata taaaaatata tttggataaa aaatgcttta tgctatttat
     1381 ttatgtgaag gaaataaaag atgatatctg ataattctag catttcgcga tcatgtgcaa
     1441 attatactta atcactcctg ttcctccaac aaattataat aatattcacg aacactttca
     1501 tcgttaaaaa ttatatttcg atttttgcga ttttcatctg attttataat cttagcaagt
     1561 tgagattctg tcattatttg ctcagtcaac caacatctaa aaaactcaaa gccatgagaa
     1621 aagccccaag ccttcttttt atgaaaacta acaactttat cgcaatacaa ctttttacga
     1681 tacttatcaa tttttttaat attaaacttc tctggtgtag attcagtatg gtaaaaataa
     1741 acttgagtgc cttgagttcc taaaatccaa ccatggctat ccttaccaag agtatatact
     1801 ccattaaatc tatagcctgt taattgaagc tcgtcataat acgcccgcgt atcaagattt
     1861 ccatttatta catcattata aagtaactca taagcttgag gtctatgcct gttgtgagta
     1921 ataatcagtg aaattgcttg aaagttccca aatacctcct cagaaataat gtcgttattc
     1981 ttaataaaat taagtattct ttcataattg agcgaatcta taatatttat agaatcaata
     2041 gtgaattcgt tataagtatt tccatcatac ttttcattac attgccgtct atagtcttga
     2101 tcgttctcta ttaaaaacga cataaatttt atagtgcttg aatctataag acatacactt
     2161 gtgtcaatta cattttttaa ctcttgccaa aagtcaagag ctaacaagct actgtcatta
     2221 taaaacttct cttttttatc tccttttcta attaataaat taacatatct aaacatattt
     2281 tgcttattag gcgaactttt tagttcacat tgtatggcgt tttttaaatc atatctgaaa
     2341 ggaattacag agccactaaa tgccttctca tagatttttg cagctttggc aaacttttcc
     2401 ttaacaatag catcttctgc tttatatatt gtagaataat atttttgcac ttttggatta
     2461 agttttccat ttattatcaa gctatctact ttatcccaag tgttttgccc taatgcaaat
     2521 ttattttgta aacagaaaca aaaaatcaaa ataataaaa
//
LOCUS       DAOPNX010000148         2454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1589
            NODE_46551_length_2454_cov_7.117966, whole genome shotgun sequence.
ACCESSION   DAOPNX010000148 DAOPNX010000000
VERSION     DAOPNX010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854794
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 2,091
            CDSs (with protein)               :: 2,091
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 10 of 17
            Pseudo Genes (internal stop)      :: 5 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46551_length_2454_cov_7.117966"
                     /isolate="AMR_MDS_1589"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PKN53_10700"
     CDS             complement(<1..715)
                     /locus_tag="PKN53_10700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD89460.1"
                     /translation="MKITIVHKSKIPAVLYGGTERVIWYLGKELTKMGHEVTYLVLPG
                     SYCDFAKIIFIDENKTINSQIPEDTDVVHFNFPVKEEILKPYITTIHGNSKAGDVFDK
                     NTVFVSKNHAERHNSNSFVYNGLDWDDYKLDKISEEKKYFHFLANAAWKVKNLKGAIS
                     VVKSANENLAVLGGKRINFKMGFRFTTSRKVKFYGMIGDLEKIKLISQSKGLIFPVLW
                     DEPFGLAIIEAFACGLPVIA"
     gene            complement(725..>2454)
                     /locus_tag="PKN53_10705"
     CDS             complement(725..>2454)
                     /locus_tag="PKN53_10705"
                     /inference="COORDINATES: protein motif:HMM:NF036482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD89461.1"
                     /translation="LWWCNGLGGQKLYDVDIQVETKFRVEECRTKIGLRKIEFISDID
                     DIGQSFYFKLNDIPVFVRGANWIPAEYFSGSNSKKTYEELLSLAKEANFNMLRVWGGG
                     IYENDDFYNICDSLGIMVWQDFIFACAMYPLDEDMEENIREEVKYQANRLYNHPSLVF
                     YCGNNEISNGWFDWNWQTQFALSYEDSLKIWNDYDKLFHRIIPNSLAQVDKTRKYIPS
                     SPFYGWGRPESLTHGDSHYWGVWWGMLDFEMFFEKTGRFMSEYGFQAFPNIESLENFM
                     HKDSLYLYSKSLKSHQKHHFGFKAIRNYMDRYFIIPEKFEDYVYMSQFLQAEGVQMAF
                     DAHLSAMPYCMGTLFWQFNDCWPSISWSAVDYYKQTKALYFYAKRSFENLSLAVFEKD
                     DKIKFVAINHFPDEVNAMAILNYKSFDGKLLCSDSLFAKINNNSVKKLNFENFSIDEC
                     NEENGFFELVLTDAIDEELLCSRTFVDDFESLALSQGNFEYKAEKQGDFWKISINSNY
                     FIKALQISTKHKGRFSDNWLDIIPGKTTIIFFYPKDKNIQNLELNFNSVNDILKREGD
                     KVENLELQN"
BASE COUNT          831 a          465 c          288 g          870 t
ORIGIN      
        1 atgcgattac cggtaaacca caagcgaatg cttcaattat tgctaaacca aaaggttcgt
       61 cccacaaaac aggaaaaata agccctttag attgagaaat taattttatt ttttcaagat
      121 cgcctatcat tccataaaac ttcacttttc ttgaagttgt aaacctaaaa cccattttaa
      181 aattaattct ttttccacct aaaactgcta aattttcatt tgcagactta acaacactaa
      241 tagctccttt tagattttta actttccatg ctgcatttgc taaaaagtga aaatactttt
      301 tttcttcact gattttatct agtttatagt cgtcccaatc caaaccatta tacacaaacg
      361 agtttgaatt atgtctttct gcatgatttt tagaaacaaa tacagtattt ttatcaaaga
      421 catctcctgc tttactattt ccatgaatag tagttatgta tggttttaaa atttcctctt
      481 ttactggaaa attaaagtgt acgacatcag tatcttcagg aatttgagaa tttatagttt
      541 tattttcatc tataaaaata atttttgcaa aatcacaata agaaccgggc aagaccaagt
      601 aagttacttc atgtcccatt tttgtaagtt ctttccctaa ataccaaatt accctttctg
      661 taccaccata caaaacagca ggaattttag atttatgaac tattgtgatt ttcattttat
      721 tatatcagtt ttgaagctct aaattttcaa ctttatctcc ttctcgtttt aaaatatcat
      781 ttacggaatt aaagtttaat tctaaatttt gaatattttt atctttagga tagaaaaata
      841 taattgtagt ttttcctgga attatatcaa gccaattatc actaaaacgt cctttatgct
      901 tggttgaaat ttgtagagct ttaataaaat aatttgagtt tattgaaatt ttccaaaaat
      961 ctccttgttt ttcagcttta tactcaaaat tcccttggct taaagctaaa gattcaaaat
     1021 catcaacaaa agttctgcta cacaacaatt cctcatcaat tgcatcagtt aaaacaagct
     1081 caaaaaatcc attttcttca ttacattcgt ctatagaaaa gttttcaaaa ttaagttttt
     1141 tgacactatt attatttatt ttcgcaaaca ggctatcact gcaaagcaat ttgccatcaa
     1201 aacttttata atttaaaatt gccatagcgt taacttcgtc tgggaaatgg ttaattgcaa
     1261 caaatttaat tttgtcatct ttttcaaaaa cagctaggct aagattttcg aaacttcttt
     1321 ttgcataaaa atacagagct ttagtttgct tataatagtc aactgccgac cacgaaattg
     1381 agggccagca atcattaaat tgccaaaata aagttcccat acaataaggc attgccgaca
     1441 agtgagcatc aaaagccatt tgcactcctt ctgcttgcaa gaattggctc atataaacat
     1501 aatcttcaaa tttttctgga attataaaat atctatccat ataattgcgg atagccttaa
     1561 agccaaaatg atgtttttga tgagatttta aagatttgga ataaagataa agagaatctt
     1621 tgtgcataaa attttctaaa gattcaatat ttgggaaagc ttgaaaaccg tattcgctca
     1681 taaatctacc tgttttttcg aagaacattt caaaatctaa cattccccac caaactcccc
     1741 aatagtgcga atcgccgtgt gtcaatgatt ctggtcttcc ccaaccataa aatggcgaac
     1801 ttggaatata ttttcgtgtt ttatcaactt gtgccaaaga attaggaata attctatgaa
     1861 ataatttatc ataatcattc caaattttca aagaatcttc ataagacaaa gcaaattgtg
     1921 tttgccagtt ccaatcgaac cagccattac taatttcatt atttccacaa taaaacacca
     1981 aactaggatg attataaagc ctatttgcct gatatttcac ttcttctcta atattttctt
     2041 ccatatcctc atctaatgga tacattgcac aggcaaaaat aaaatcctgc cataccatta
     2101 ttcctaagga gtcgcaaata ttataaaagt catcgttttc ataaattcca ccaccccaaa
     2161 ctcttagcat attaaaatta gcttcttttg ctaagcttag caattcttca tacgtctttt
     2221 tcgaattact tccgctaaaa tattcagcag gaatccaatt tgctcctctt acaaaaacag
     2281 gaatatcgtt aagtttaaaa taaaaacttt gccctatgtc gtctatgtcg cttataaatt
     2341 caatttttct caagcctatt tttgtacgac attcttccac cctaaacttt gtttcgacct
     2401 gaatatcaac atcatatagt ttttgcccac ctaatccatt acaccaccaa agtt
//
